LOCUS       DAOQQN010000001        27553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_516_length_27553_cov_5.794203, whole genome shotgun sequence.
ACCESSION   DAOQQN010000001 DAOQQN010000000
VERSION     DAOQQN010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 27553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27553
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_516_length_27553_cov_5.794203"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3546
                     /locus_tag="PLG24_00005"
     CDS             <1..3546
                     /locus_tag="PLG24_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ23288.1"
                     /translation="IPCYSDVNFANINVDDCSTPVQSHIISMITQNLNCDGDPLGIIR
                     RVIRTYYATDAAGNVSDTCTDTVLVQRPVLDSVTCPDNDSLRCDANYAKDAFGNPDPS
                     VTGVPTIGLGHHPLFPNTLLGACNLYTSYKDEITDFGCSIRIKRTWTITEGCCNDNHE
                     RQCVQIICLYDDVPPVLEVPDNFTVSTGAYACTANVTIPAATATDNCKTQLNWVVTYP
                     GGYKNQNGGFNVTLPIGTHNITYTVNDGCVQNTVGTMQITVVDNVAPNAVCIENTVAS
                     IPNGAQYVRVYASSFDNGSWDNCSDITLKVARMNPDCNGDLDPQITERDYIDFYCCDV
                     SPTNSITVRLFVYDAAGNKSECMVQIKIQDKTMPILVIPPDMWVPCEFNYNPDDLGST
                     FGYVLTDGSPRREDDIRTGLSGPIVHTYLDGYATDNCGVDLDEDVSEDFDDCGAGTIT
                     RTFTATDPFGNTVVKKQTIHFYKTPLNLSSSLFIAPRDTMIVNGTCTVDGLQPDDLAA
                     EYSPRFTLSPVTTCFNLAYNYRDEVYYGADDACFKIIRYWTIMDWCYASVYGLDAALA
                     TAVHFTQMIKVKNEVAPTFDPLADITAISEDSDCFNEFVQVSNIAHDDCTPDNQLTYK
                     YKIDLNYSVSGPATWDYTGSGNAIARNFPIGVHRICFYATDHCGNVGESCIILTVINR
                     KKPTPVAHMLVTEIMPTAGTITLPAWYFDAGSWANCGGPVWFSFSSNVNDTLHTFTCD
                     DIGNNPPASIQFWVTDQYGNQDYVTVQVIIQDNNGVCDDNLTFVAGNILTENSQGIPK
                     VDVEGPSNTSTSNQGTFRFSSITLGQTYTITPKSERDPLNGVETGDIIKIQNHILNKK
                     ALDGPYKLIAADVNQDNKITVNDIVVMRKLILGKIDQFPSGQSWRFIDKKFQFADQTN
                     PFKAAFPEHISVTPNGTVNNVDFYGVKLGDVNNNVTLNFGDQSVETRNNETVTFSAQE
                     QNLTAGVPSEVTFKASDFAKVGGFQFALAAANGVKVINATSNDMTFNEDNFNVMSDQT
                     TRISWNTENASVTEGSVTLTIVTDRDMKLSEAISLSSTDLTAAAYDQTGAEMNVALKF
                     TGTNADAVTVFQNRPNPFSDETRIGITLPENMDVTLKVYDLSGRTIYQTTKEFAKGSN
                     EILINSDMINASGVLFYEVSTKFGTEMKKMIRLKN"
     gene            4022..6448
                     /locus_tag="PLG24_00010"
     CDS             4022..6448
                     /locus_tag="PLG24_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HMZ23289.1"
                     /translation="MHAQQFMMQGWYWDYPKTAAGKNWADSLNLKVEGLKNAGFTHLW
                     LPPHTASASGQNSNGYDPKDLYIGNTTTGFATRPSLDALLNHMVSIGLNPVADMVMNH
                     RDGGMAEDNPAVKSYISSYDINKANNGDNPFPSDRFRCLLPLGGSSGNGAGDYYFKLS
                     SASGHSRFFNKQYKLYMQTSKVGYQNLPSLNEVEPNGGGDCVEGNNNLPLGVDLFGNI
                     DALGCRTDEFHVHLDAGDFNPAGDYLEIYMSNPNGNYSDHRFYGIWRATPGADVVSQL
                     VYQTYTDFSGMPSAMGEMHWDCFKPNFTNSTGLQGDQDGMFFFYDYDQNTTCARDELF
                     AWAKWNCETLGIKGFRLDAVKHFPASFVGDLLDYLHDEGIDLDLIVGEWYGTNTGELA
                     GWVNDVLSAMDADTKAAMHPKIFDFALRESLRQACDVYGYDARNIFTSSLNAAGLSGY
                     HIVTFVNNHDFRDATGFASLVDNDIMLPYAYILTNNQLGVPCVFYPDYYGYYGNASGR
                     SIPPPPLKPYIDQLVKVHQQYIYGASTAVYLTQIGSSYSTTFNSGGPSTTVIYQLQGG
                     IAGKDVVVAINFAGEDLDVEQVINTTNLTEGTKFTDVTGLSNGPEQITLGSGKLNLKL
                     KARSYAVWVQGENPRIPLPVHVISLDARKNNKVVKINGKLTGLDATEKIILQKAIDPS
                     HFVDFQVLDYSAGDADFAFASEDNTPAFYNYYRLKLMGKDERVNYSEIKSVRFGMVTV
                     KLINTLAEAKLFLSGLDENMHFYEVMNIHGKKIISGRLYDTMIPVDALIPGVYFLRID
                     RNTVYKFVKP"
     gene            complement(6641..7864)
                     /locus_tag="PLG24_00015"
     CDS             complement(6641..7864)
                     /locus_tag="PLG24_00015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HMZ23290.1"
                     /translation="MNRVVITGMGIYSCIGLNKDEVLESLRQGRSGITYDAERKDFGY
                     RSALVGLVPRPDLKGHLDRRARIMLPEEGEFAYIATKEAFTQAGITPEILKDKECGII
                     FGNDSTVKAVYDSIELVKQKKDTTLLGSGAIFQSMNSTVNMNLSTIFALKGVNFTLSA
                     ACASGSHSIGIGYLLIKQGLQKMVLCGGAQEVNIYAFASFDGLGTFSIRESEPEKASR
                     PFDRDRDGLVPAGGAAALVLESLESALARGANIIAEVIGYGFSSNGEHISGSSKEGQV
                     RSLRMALEQAGLKPKDIDYVNAHATSTPAGDTQEAQAIAEVFGDKEVPVSSTKSMTGH
                     ECWMAGASEAIYSTLMLNNNFIAPNINFEHPDEHSAKLDIVTKATEKELNIVLSNSFG
                     FGGTNSTLILKKFIE"
     assembly_gap    8776..8875
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9265..10548)
                     /locus_tag="PLG24_00020"
     CDS             complement(9265..10548)
                     /locus_tag="PLG24_00020"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HMZ23291.1"
                     /translation="MTEIRVWCLLLLGILARTSGYTQPTELVNYDYNYDDKIKTVILR
                     PYGSTVGFPMTYLNSDRPLELKFDDLNGDFVYYKYRIVQCDANWNPSQLNELEYLEGF
                     NDQEIREYAYSINTRVVYTTYSLVFPNDLISVTKSGNYLIHIYRDDDNMPVLTRRFVV
                     SEQLFGVAATLASSLEAGKMREDQRIDLSLTTSQRIQDPLNQVKIAILKNGIWNNRPL
                     LGPRFVRNDRMTYDYIKETSFPGGKEFRYLDIRSVRRPNYKIENLERFNDRTEVFLRT
                     ENLEGRSEHSSYPDLGGSYLIQNLDRPDSEMENDYFLVHFYLKSPGRLSNPVYVLGQF
                     NQWGTSEDAQPMKYEPEKGYYTTTILMKQGYYDYYYAEKTKSGFNSNETEGNSYETAN
                     IYQIIVYYHAFGERWDRVGQFWTLGTPVSGALRED"
     gene            11199..11597
                     /locus_tag="PLG24_00025"
     CDS             11199..11597
                     /locus_tag="PLG24_00025"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HMZ23292.1"
                     /translation="MNPGQYIILYDGDCGFCNQSVMFIIRNDRQKNFLFASLQSEIAG
                     DLLKPFNIRPSLNSMILLKEGQYYEKSGAALEIVQHLSGVWPLLSLLKIIPRPIRDGV
                     YCWIARHRHRFLSDTACTLPIPDVESRFLK"
     gene            complement(11690..13048)
                     /locus_tag="PLG24_00030"
     CDS             complement(11690..13048)
                     /locus_tag="PLG24_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMZ23293.1"
                     /translation="MKIRVKLTLQYFVVVATILILSMFFVYRRFQDITYNEYYNNLKT
                     KAHMTAEMVLHKGDLDKLEDITPHKDSENLPMSDNVIIFNNNYEKVFTFNRKSAFVLP
                     FDIRPISKREFSYHTGDMYYFGLPYTSMSGRKYYVVASSVFNSKELMDLKRIISLAII
                     LGLVVVGVSGFFYTRQALNPLFSIVHQMDQLDMSKLDSRLKRSYTKDEIEHLIQSINN
                     LLSRIQSSVTFQKMFISNVSHELKNPISIVLSQVDVLLNQKDRTVDEYRATLTSLRDD
                     VQDIAYITENLLQMAKIKSEGFHLDIQNFQLDEAILECQSKLLRSHPEYSVSFQIEGQ
                     PDSQEKLTVEGNEALLKLAFINIMDNCCKYSDDKHVDIRINSTDRGLELSFRDKGAGI
                     AKEDMEMIFQPFYRNINTKHVKGYGIGLSLIDAVLQVHKLKLKIESTPKVGSLFAIPF
                     TR"
     gene            complement(13045..13737)
                     /locus_tag="PLG24_00035"
     CDS             complement(13045..13737)
                     /locus_tag="PLG24_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ23294.1"
                     /translation="MKILIVEDEKKLNSSLKKGLEEQGFAVDVAYNGIEGKKLAFANQ
                     YKVIVSDIVMPQSTGLQMLKALRQNNIHTPVLMLTALNMVEEKLEVYAAGADDYLTKP
                     FDFRELVARIRALIKRLPKVAAPSQSEITYADITINPATKKVRRQDINIFLTPKEFEL
                     LHYFMTHPNTTITKQELLSNIWDFDFETSTNVLEVYINFLRNKIDKDFSEKLIHTLPR
                     IGYIFKIQHEEQ"
     gene            14128..15117
                     /locus_tag="PLG24_00040"
     CDS             14128..15117
                     /locus_tag="PLG24_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23295.1"
                     /translation="MSVRLSVSKLIALWAFSESALGGLLHATNMPFSGLLLGGIAVTV
                     ICCIGFTAVRPGAAILYGTLVAVSVKFALSPHSPVTSYIAVGFQGIVGALMFSFQRRS
                     LPVYIFYGGIALAESALQKVITLTVIYGNALWDSLNVAAQQIAKSLHMDSSLSYAVIL
                     LCIYSMVYFVWGCLIGYWAHKIPHEIKNIDKSEIISIDTSGKLFNKKKRKIRYLVWMP
                     MVLILSVSVILMRQSGLSVLQLILRPFIVVMVWWFVINPFLKAMLARFLNGRSNNMAR
                     IIDSIRQDIPRLRKLSFQTADYVTRHYRGVSRVRRFITILIILASEEKSNLTG"
     gene            15089..15556
                     /locus_tag="PLG24_00045"
     CDS             15089..15556
                     /locus_tag="PLG24_00045"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HMZ23296.1"
                     /translation="MKKKVILLDNLWVITGEVNSGKTTSLLQWTRGRKVAGFLTPDID
                     GQRRLMDLSTSEILPFEANDDDEDVQKVGRFSFFQSAFDYGNSLIIHSLQADHPYFII
                     DEFGKLEINGQGFAYGIDTLMGGIRTSQSPTIYIIVIRESLLDGFLERYGLSD"
     gene            complement(15990..16487)
                     /locus_tag="PLG24_00050"
     CDS             complement(15990..16487)
                     /locus_tag="PLG24_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23297.1"
                     /translation="MKLLIKQGLTFSALLISFLLTLSFIGCNRSQKEGPSDPVSPQDT
                     GQVIQFSEVDRATARTAIHTYRGPTQQNAGFSFVDVTRDEFQALQQMFNDTTSKYDKV
                     RIYFGIDTFRLDASPTKSFFTGFVGKAEKGARGDSTSFIIVQRIIKGSAPCPKTCDFT
                     KSIFR"
     gene            16626..18419
                     /locus_tag="PLG24_00055"
     CDS             16626..18419
                     /locus_tag="PLG24_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23298.1"
                     /translation="MSTDPFLAICRTFLFLTIIMLGMLGCKRGSEERHNDSKIVTNDF
                     GQGIPGILYRAKVQLDSFGFVNKADSLLELAINEAVKAGKNDQIAMAYTFALKYSSSK
                     PVNEAQALEFYNTGKYHAGLSGSISLLNELNFAMAHYYTKRGNTGQSEKYLNQISFFG
                     GSNFELYLKKEMAEAANFGVRDKPFEQLKSLLDAMYKAYELGHDSIYQACLLNISDFY
                     YYEQQYNKALDYIDQLQNTIAAKGIQTDSNRIYFLMANKLPIFNKQFDQNATIRLAES
                     ISHYARKHGYQLLEEIAQGQVRTALISSGDFKRVEDYYRAYPVYLSRLEHEKPSIYYR
                     MLSYIEMAKGKKDSAVAYFDKAGEFLDGENEYYVANYHFRKGELMEKLGQRSESISSY
                     SKALKMLNNSHDLTLQNTLIDKLIPYYKEINDPRSIVGLLEKKDSLNMKLIQINKEQN
                     IRQLELSNILSVYELKHQKAAEELNRKQVIQLQIVALFILLVFMSLLVMANLKLPGWW
                     LRSMGYISIIILFEFIILMVDRRLHDMLHGSVIKLLVIKVILISWILPLHHWAEKKIV
                     AFLLQKDRFRGLKDFLSRLFQSFGKKEVEES"
     gene            complement(18480..19196)
                     /locus_tag="PLG24_00060"
     CDS             complement(18480..19196)
                     /locus_tag="PLG24_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23299.1"
                     /translation="MDFTSIYDPSVFDRFLTIGSGSYREVIHFYEDHRNKIENLDARH
                     RFEIYVCYIEALFEMGKFKEVLFLVDDAIETAIELGFSRLFGVDIYFNLLMKKAVALN
                     EAGRVEDSLSIIRQLLSMNPDEPNVRYFFKEIMMNRSRKLVLPTRVFFIAGVITTTIL
                     MAIEALVIMPFYSDLIDPVSGVRNVLFLFSVLIFISGEVSRFLLIEWHCLLLIRSIKK
                     HRPTNTIKGSNTRRVIRHKK"
     gene            complement(19198..19971)
                     /locus_tag="PLG24_00065"
     CDS             complement(19198..19971)
                     /locus_tag="PLG24_00065"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HMZ23300.1"
                     /translation="MNVETYNIEVSVTLNGQKYTADLHHPIDISMILHSGYPQVNCFR
                     APYFYASPHREGDLVKSIAQGGHANFYNVTIHPHGNGTHTQCVGHIVRDQYYINEYYK
                     PDFHVAQLVSIFPQLSDNGDKVIHAEQLSQLWEAVGEEALIIRTLPNGPEKRKTEYTD
                     TNPPYFSRDAIDLIVSRGIKHLLTDLPSLDREENQGKLEAYRAFWNYPDIDLRAGATV
                     AELIFAENFVEDGLYLLNLHLLNIQLDVSPSRPFLYRLR"
     gene            20204..20827
                     /gene="tmk"
                     /locus_tag="PLG24_00070"
     CDS             20204..20827
                     /gene="tmk"
                     /locus_tag="PLG24_00070"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HMZ23301.1"
                     /translation="MAKPVFIVIEGIDGSGKSTQIELLQHRMSEEGKQVAITGEPTDG
                     FIGKIIRDIMRGKEETDPAVVAALYVADRMDHITHPDVGMLALLEKGYNIIASRYYFS
                     SYAFQGEYVSLEWLIHANSICKSRLKADLTVYINIDPAICFERIKSSRPSLDIYENLE
                     KMKRTHREYMKAFEVAGMDENIVMVDGTLPIDLLHEAIWSKVKEVAF"
     gene            complement(20938..21822)
                     /locus_tag="PLG24_00075"
     CDS             complement(20938..21822)
                     /locus_tag="PLG24_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HMZ23302.1"
                     /translation="MSLFRILPDRIDVVQSQNRPDDKNSWQYKLYVIIFQANTKAGKM
                     FDIILSLMILINIFIILAESIESLSAKYSFYFRQADRFFLFVFTLEYLLRLSCVKVKK
                     RFVKSTFGMLDLIAILPSYFEYFLPQSHMLMIIRSFRLLRVFRIFGMVKFLDESRELV
                     FALIRSFRKIAIFLFFILILAFLLGSLMYLAEYQHNPNFHSIPQSIYWAIVTLTTVGY
                     GDITPITPIGKILASFIMILGYSIIAVPTGIIGGNIIKEMKKTNINLVCENCGTKEHS
                     NDAVYCRQCGERLHQPVI"
     gene            22567..25404
                     /locus_tag="PLG24_00080"
     CDS             22567..25404
                     /locus_tag="PLG24_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23303.1"
                     /translation="MRIFILSIICCISFVFALPAQQGVYNMVDFYRELSVGHPVLPFP
                     ENVKFESGLNKYIGEPMALLGQRAPGSRSGQQNEMSAVTMTEIEHLFSGRIQRGVVHS
                     ANQPPVADAGPDTTIVLPVDSVLLNGSGTDPDGTIVSFEWTPDPNNPSAATFDDASLP
                     NPMVSNLIEGIYIFTLTVTDDQGEIGSDQVTITVQAANQPPVVNAGDDLTIHLPESSV
                     QLTGTAIDPDGSVVSFSWSQDPSNPAPAAFSNFSIPNPVVSGLIEGVYSFTLTAVDNE
                     GATGQDNVLVNVLPPINLPPDAHVGFDLRVILPTDSTILHGSGTDPEGAPITFLWKQI
                     GDLPSIATISDSASSDPVVSDLILGIYTFRLIVTDDKGLSDSVEMNVIVDIVGNIKPI
                     ANAGEDATILVPQTSYQLDGSGSDSDGFIIGYNWEQIGTLPNTAIISNPGVPNPIVSG
                     LIKGAYTFRLTVTDNEGGKGMNDMVLIVFDTFNIAPIANAGPDATIALPQDTYQLSGS
                     GTDADGIIISYHWQQVGNTPSQAVFSNTDIPNPVVSGLIYGHYTFRLTVKDDYGLTAS
                     DDIVLFVRDSFNHIPVANAGENIRLTLPDNSVQVHGTGYDDDGIIVGYEWQQVGNKPS
                     KAVISNPDIADPIIGGFLYGTYTFVFTVRDNEFATGSDTLLIVVDTVPLIAPIADAGA
                     DISVNLPQDSVQLHGSGSDEDGTIVGYTWYQLGTQPSPAAFSDTMIADPIVKNLVEGT
                     YHFVLKVTDNDNLMDTDTVRVVVSKAKNIPPVANAGEDITIKLPIDATTLHGSGSDDD
                     GTVTDFLWEQVGTTPDIATIIGNNSPEPTVSNLFKGTYTFRLIVTDNEGAKDTDEVKV
                     FVLDKLSTHTGDVSSAIYPNPAKGGIFHINPGAKLKPGTSARIFDIDGREMMRFVVGR
                     EDSSFQCNQCVSGVFVIRFDNGMSAALVVMP"
     gene            25998..27131
                     /locus_tag="PLG24_00085"
     CDS             25998..27131
                     /locus_tag="PLG24_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23304.1"
                     /translation="MLTIQDIQGSDNVKKEITTLISSGQFPHTTLFIGPEGSEKLNLA
                     YATARLLMCENNKNGVICGQCNSCRKSANFTHPDIFFSFPFVKEGSGDFQSDDFLQAW
                     KEAFRENPYMNVYEWSLKQDAGNKQPNITAFECIQIQRKLQKKPYESDRKVLLLWMPE
                     YLGKESNRLLKILEEPPGDTFIILVAEDQERLLATILSRCQLFKVEPFDLQGVQRILE
                     NHFGKSKEEATMAAYLSRGSMVEALSLFSEGNLEKNGEFVTWLRLCYSFNHVEIIRWI
                     EHFATYTREVQKYIFKFGIHFVNEMLKIKYNYGVDNISLPKEMADVAARLCPLLSFES
                     IEELVNTLDREIFLTERNCNTKMQLIYTSGYIHRLFNGKRFAA"
     gene            27155..>27553
                     /locus_tag="PLG24_00090"
     CDS             27155..>27553
                     /locus_tag="PLG24_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23305.1"
                     /translation="MGCNSCSTEDGKPKGCRSNGVCGTSGCNKLNTFDWLTTMELEDP
                     TEVNLVEVSFRKGAHKSFFRLQDAYQAEIGDDVVVDADGGYDIGRISLKGALVPIQMK
                     KKSVDPNSALRFVIRTASQNDMAKLEQAISM"
BASE COUNT         7794 a         5501 c         5978 g         8180 t
ORIGIN      
        1 ataccatgct attctgatgt aaactttgca aacatcaatg tcgatgattg ctcaactcct
       61 gttcagtctc atattatatc catgattaca cagaatctga actgtgacgg tgatccactg
      121 ggtataatcc gcagagtaat cagaacctat tatgcgactg atgctgccgg taatgtcagt
      181 gatacttgta cggacacagt actggttcaa aggcctgttc tggattcggt aacatgtcct
      241 gacaatgact cattgaggtg tgatgccaat tatgccaagg atgcctttgg taatccagac
      301 ccatctgtta ccggagttcc aactatcgga ctaggccatc atcctctctt ccccaatact
      361 cttttaggtg catgtaatct ttacacaagt tacaaggacg agattactga ctttggttgc
      421 tcaataagaa tcaagcgaac atggactatt acagagggtt gctgtaacga caaccatgaa
      481 aggcagtgtg ttcagattat ttgtttgtat gatgatgtgc ctccagtcct tgaagtgcca
      541 gataacttca cggtaagtac tggtgcttat gcatgtacag ctaatgtaac gattcctgct
      601 gcaaccgcta ccgataattg caagactcag cttaattggg tggtaactta tccaggcggt
      661 tataagaacc agaatggtgg attcaatgta accctgccaa ttggtacaca taatatcaca
      721 tatacggtca acgatggttg tgtccaaaat accgttggta caatgcagat aaccgtagtt
      781 gacaatgtgg ctccaaatgc tgtatgcatt gagaacacag ttgcttcaat tcctaacggt
      841 gctcaatatg ttagagtata tgcatcttcc tttgacaatg gttcttggga caactgtagt
      901 gatatcactc tcaaagttgc aaggatgaat cctgactgca acggtgatct tgatcctcag
      961 attactgaaa gagactacat cgatttctat tgctgtgatg tgagtccgac caatagtatt
     1021 actgtgagat tgtttgttta tgatgctgcc ggtaataaga gtgaatgtat ggttcagata
     1081 aagattcagg ataagaccat gcctatcctt gtgatacctc ctgatatgtg ggtgccttgt
     1141 gaattcaatt ataatcctga tgatcttgga tctacttttg gttatgtgtt gactgatggt
     1201 tccccaagaa gagaggatga tatccgcaca ggactgtctg gaccgattgt acatacctat
     1261 ttggatggat atgcaactga caattgtggc gtagatttag atgaagatgt ttctgaagat
     1321 tttgatgatt gcggagcggg tacaattact cgtacgttta cggctactga tccatttggc
     1381 aatactgtag tcaagaaaca aacaatacat ttctataaga ctccattaaa tctgtccagc
     1441 agcttattca tagctcctag ggataccatg attgtaaatg gaacttgtac tgttgatggc
     1501 cttcagcctg atgatttggc ggcagaatat tctccaagat ttacattaag tccggttaca
     1561 acatgtttta acctggcgta caactatagg gatgaggtat attatggtgc tgatgatgct
     1621 tgcttcaaga taattcgtta ctggactatc atggactggt gctatgcttc agtgtatggc
     1681 ttggatgccg cattggcaac agctgtgcac tttacccaaa tgattaaggt gaagaatgaa
     1741 gttgctccta cctttgaccc attggctgat attacagcaa tctccgaaga ttctgactgc
     1801 tttaatgagt ttgttcaggt ctccaatatt gcccacgacg attgtactcc tgacaatcag
     1861 ttgacttata agtataaaat cgacttgaac tactctgtct ccggacctgc tacatgggat
     1921 tataccggtt caggaaatgc tattgcacgt aacttcccaa ttggtgttca cagaatatgt
     1981 ttctatgcca ctgaccattg tggaaatgtg ggcgaatctt gcatcatact tactgtaatc
     2041 aacaggaaaa agcctacacc tgtagcgcat atgttggtaa ccgaaataat gcctactgca
     2101 ggtacaataa cattgccggc atggtatttc gatgcagggt cttgggctaa ctgtggtgga
     2161 ccggtctggt tctcattcag ctcaaatgtc aatgatacac tgcacacatt cacttgtgat
     2221 gatattggaa acaatcctcc tgcatcaatc caattctggg tgactgatca atatggtaac
     2281 caggattatg taacagttca ggtgattatc caggataaca atggcgtatg tgatgacaac
     2341 ctgacattcg tagccggaaa tatcctgact gaaaactctc agggtattcc taaggtggat
     2401 gttgagggtc caagcaatac aagcacatct aaccaaggta cattcaggtt tagttccatc
     2461 accttgggac agacatacac aatcactcct aagagtgaaa gagatccact gaatggtgtt
     2521 gaaacaggcg atattatcaa gatccagaac catattctga ataagaaagc tctggatggt
     2581 ccatataagt tgattgctgc tgacgtaaac caggataata aaattactgt aaatgatatc
     2641 gttgtaatgc gtaagctgat tttgggtaag atcgatcagt tcccaagcgg acaaagctgg
     2701 agatttattg ataagaaatt ccagtttgct gaccagacca atccattcaa ggcagcattc
     2761 cctgagcata tctctgtaac tcctaacggt actgtcaata acgttgactt ctatggtgtg
     2821 aagctcggtg atgtcaataa caacgtaaca ttgaactttg gtgaccagtc cgttgagacc
     2881 agaaacaatg agactgtgac tttctctgca caagaacaga atttgacagc aggtgtacct
     2941 tctgaggtaa cgtttaaagc aagcgatttt gctaaggttg gcggattcca gtttgctctt
     3001 gctgctgcaa atggtgtgaa agtaatcaat gcaaccagca acgatatgac tttcaacgaa
     3061 gataacttca acgtaatgag cgaccagact acaagaatat cttggaacac tgagaacgca
     3121 agcgtaactg aaggttctgt tacattgact atcgttactg acagagacat gaagttgagt
     3181 gaagctatca gcttgagctc tactgacctg actgctgcag cttacgatca gacaggtgct
     3241 gagatgaatg tagctctgaa gtttacaggc acaaatgctg atgcggttac tgtattccag
     3301 aatagaccga acccattctc tgatgaaaca agaataggta tcacattacc tgagaatatg
     3361 gatgttacct tgaaagttta cgacttgagt ggacgtacta tctatcagac aactaaggaa
     3421 tttgccaagg gatcaaatga aatcctgatc aactccgata tgatcaatgc aagtggtgta
     3481 ctcttctacg aagtaagtac caagtttggc actgagatga agaagatgat aaggcttaag
     3541 aattaagcaa tatactgttt ttgggatgag ctccgttccg gtaaaagtac cggacggggc
     3601 ttttttattt tacataatta ataatattga gtctactgtc aagttaccat actgccacct
     3661 atagcagaaa agatttgccc tccaattctt tgaacttacc acttaggaaa ttcccgggat
     3721 ttttttcaga ccaagatcat atacaattga ttcatattca ttatcctgga ctgtcctgct
     3781 ttgcaataac cgtctaaatg tgttgaaagg gaaacttctt taagttttaa ttacattcct
     3841 accggaatat catttagaag gtatcctcca agaaaaattt tattgtactg aagtttattt
     3901 gagagaaaat attagggtaa aaatcttagt tttgttctga tattacaatg attattggaa
     3961 gatgaggctg aatattttcc gtttatgctt gttggggtta ctggtattaa caggatcaga
     4021 tttgcatgca caacaattca tgatgcaagg ctggtattgg gattatccta agacagctgc
     4081 aggcaagaac tgggcagata gcctcaattt gaaagttgag gggttgaaga atgccggatt
     4141 tacacacctc tggctacctc cccatactgc ctctgcctcc gggcaaaata gtaatggcta
     4201 tgatccgaaa gatttataca tcgggaatac tacgaccgga tttgccacta gaccatcgct
     4261 ggatgccctt ctaaatcata tggtctcaat cggcttaaat ccggtagcgg atatggtgat
     4321 gaatcaccgc gatggtggaa tggctgaaga taatcctgct gtgaagtcat atatctcaag
     4381 ttatgacata aataaggcaa ataatgggga caatccattt ccatcggata gatttcgttg
     4441 ccttcttcct ttgggtggat caagtggtaa tggagcggga gattattact ttaaattgag
     4501 ttccgcttca gggcacagtc gtttttttaa taagcagtac aagttgtata tgcagacctc
     4561 aaaagtaggc tatcaaaacc ttccttcttt gaatgaagtg gagcccaatg gtggcggtga
     4621 ttgcgttgag ggcaacaata acctgccatt aggagtggat ttatttggca atatagatgc
     4681 gcttggatgc agaacggatg aattccatgt acatctggat gcaggagatt ttaatccggc
     4741 aggagattat ctggaaatat atatgtctaa ccccaatgga aactattctg atcatcggtt
     4801 ttatggaata tggagagcca ctccgggcgc tgatgtggtt tcacagttgg tgtatcaaac
     4861 ctatactgat ttctcaggta tgcctagtgc aatgggtgag atgcactggg attgtttcaa
     4921 acctaacttt accaactcta ccggccttca aggtgaccag gatggcatgt tcttttttta
     4981 tgactatgat cagaatacaa catgtgcccg ggatgagtta tttgcctggg ctaaatggaa
     5041 ttgtgagacc ttgggtataa agggattcag gctggatgcg gttaaacact tccctgcttc
     5101 ttttgtgggt gatttgctgg actatctgca cgatgaaggc atcgaccttg atttgatcgt
     5161 cggggaatgg tatggaacaa atacaggtga acttgccgga tgggtcaatg acgtccttag
     5221 tgctatggac gccgatacca aagccgccat gcacccaaaa atattcgact ttgctttgcg
     5281 ggaatcacta cggcaggcat gtgatgtata tggctacgat gcccgaaata tctttacgtc
     5341 atcgctcaat gcagcaggct tatccggcta tcatattgtg acctttgtca acaatcatga
     5401 ttttagagat gcgactggat ttgcgtcatt ggtagacaat gatataatgc tgccttatgc
     5461 ctacatcctc accaacaatc agcttggtgt tccttgtgtt ttctatccag attattatgg
     5521 atattatgga aatgcaagtg ggcgatcgat tccgccacct cccctcaagc catatattga
     5581 tcagttggtc aaggtacacc agcagtatat ttatggtgca tcaaccgcag tatatttgac
     5641 tcagatagga tcttcttata gcacaacatt taattcaggg ggaccatcga cgacagtaat
     5701 ctaccagtta caaggaggaa tagcaggaaa ggatgtcgtt gttgcaatca attttgccgg
     5761 tgaggatttg gatgtggaac aggtaatcaa tacaacaaac ttaaccgaag gtaccaaatt
     5821 tactgatgtg acaggcttga gtaatgggcc ggagcagatc acactcggct caggcaagtt
     5881 gaaccttaaa ttaaaagcaa ggtcatatgc cgtgtgggta caaggtgaga atcccaggat
     5941 accattacct gtacatgtta tttcactgga tgcgcggaag aataataagg tagttaaaat
     6001 caatggtaag ctgaccggtc tggacgcaac agaaaagatc attcttcaga aagcaataga
     6061 tccaagtcat tttgttgatt ttcaggtact cgattatagc gcaggtgatg cagattttgc
     6121 ctttgcatca gaagataata cacctgcttt ttataattac taccgactta aattgatggg
     6181 taaagatgaa cgtgttaatt acagtgagat taaaagtgta cgcttcggaa tggtgactgt
     6241 caagctgatt aatactcttg cggaagcaaa attattcctg agtggcctcg atgagaatat
     6301 gcatttttat gaagtgatga atatccatgg aaagaaaatc atttcaggac gactctacga
     6361 tacaatgatt cctgttgatg ctcttatacc gggcgtttat tttctacgta ttgacagaaa
     6421 tacggtgtac aagtttgtca agccttaatt ttcatttata ccaaaaatag atattatacc
     6481 aggatgattt tataataatc tataaatttt tggtataatg ccgagattac agtcgtatag
     6541 acccatgaaa ttgggctata acgaatgtac cttcggtatt ggcactgcaa ctaaaagaag
     6601 cagtatcaac atcggaatat ccatctcaaa tagagaaaat ctactcaata aactttttta
     6661 aaataagcgt tgaattagtt cctccaaagc cgaaactgtt actgagtacg atattaagtt
     6721 ccttctcggt tgccttggtt acaatgtcca attttgctga atgttcatcg ggatgctcaa
     6781 agttgatatt cggtgcaata aagttattgt tgagcatcaa agttgaataa atagcctcac
     6841 tggcacctgc catccagcat tcatgtcctg tcatggactt ggtagagctg acaggtactt
     6901 ctttatctcc gaaaacttct gcaatagcct gtgcttcctg tgtatcacct gccggagtgc
     6961 tggtcgcatg tgcattgaca tagtcgatgt ccttaggttt cagacctgcc tgttccaaag
     7021 ccattcttag tgaacgcacc tgcccttcct tactggaacc ggaaatatgt tcaccgttgg
     7081 aagaaaagcc gtatccaatc acttcagcaa taatattggc gcctcgggcc aaggcacttt
     7141 ccagtgattc aagtactaag gccgcagctc cccccgctgg taccaagccg tctctgtccc
     7201 tgtcaaaagg ccttgatgcc ttttctggtt cggactcgcg tatggagaat gtgcccaggc
     7261 catcgaagct ggcaaatgca taaatgttga cttcctgagc acctccgcat agaaccatct
     7321 tttgaagtcc ttgcttaatt aaaaggtagc cgataccgat ggaatgtgaa ccgctggcac
     7381 aagctgcact caaagtgaag ttgactcctt tcagcgcaaa gatggtagag aggttcatat
     7441 tgacggttga attcattgat tggaagattg ctcccgaacc caggagggtg gtgtcttttt
     7501 tctgtttcac cagttcaatg gaatcgtata ctgccttgac tgtgctgtca ttgccaaaga
     7561 taatgccgca ctccttgtcc ttgaggatct cgggtgtgat tcctgcctga gtgaaggctt
     7621 cctttgtggc gatataggca aattcacctt cttcgggcaa catgattcga gcccgacggt
     7681 caaggtggcc tttcaggtcg ggtcttggta ccagacccac caaggcagaa cgatagccga
     7741 aatccttccg ttcggcatca taagtaatgc cagaccttcc ctgtctgaga gattccaata
     7801 cctcatcctt gttcaatccg atgcaggaat aaattcccat gccggtaatt acaactctgt
     7861 tcatacttca aaaataaact tattaactaa ttgttgcaat aattgaagaa atagatacaa
     7921 ccttatacaa ggaaaatata aaaagttgtc aaggaatcta tatttggcgg gttcaatgga
     7981 tgcttgtagg agtagtaagt tgtgggagag aggaataaga atgagtaggt tgaatttttc
     8041 aagtaggtag atgattaata atctcattgt catagttggt gcttattgcg aagattttga
     8101 tttttaattt aggtgtaaat tatgggagaa attcaattgc aaggtgcagc atacgcacgg
     8161 ggactggagt ttcagttatt aatttcctac ctactacatt ttttccgccc tgggaagttg
     8221 tggtctcttc gaagtaggta gatgattaat aatctcatag tcatagttgg tgcatatcgc
     8281 gaagattttg atttttaatt tgggtgtaaa ttatgggaga aattcaattg caaggtgggg
     8341 tatgcccttc aggaccggag cttctgttat taatttccta cctactacat tttttccgcc
     8401 ctgggatgtt gtgttctctc ggaagtaggt aaatgaataa taatctcatt gccatagctg
     8461 gtgcttaccg tgaagatttt gatttttaat ttaggtgtaa attatgtgag aaattccctt
     8521 gcaaagtgtg gtatgccctt caggaccgga gcttcagtta ttaatttcct acctacttca
     8581 ttttttccgc cctgggaagt tgtgttctct ctgaagtagg tagatgatta ataatctcat
     8641 tgtcatagtt ggtgcatatc gcgaagattt tgatttataa tttaggtata aaatatgaga
     8701 gaaattccct tgtaaagtgt ggtatgccct tcaggaccgg agcttctgtt attaatttcc
     8761 tacctactac attttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagatg
     8881 attaataatc tcattgtcat agttggtgca tatcgcgaag attttgattt ttaatttaag
     8941 tgtaaatttt gagagaaatt caattgcaag gtgcagcata agcacgggga cttgagcttc
     9001 agttattaat ttcctaccta cttcattttt tccgccctgg gaagttgtgt tctcttcgaa
     9061 gtaggtagat gattaataat ctcattgtca tagttggtgc atattgcgaa gattttgatt
     9121 tttaatttag gtgtaaattt tgagagaaat tcaattgcaa ggtggggtat gcccttcatg
     9181 accggagctt cagttattaa tttcctacct acttcgtctt accccttcga ttctattcaa
     9241 atgtattccc ttcttctttt ttcctcaatc ctccctcaat gctccgctca ctggcgtgcc
     9301 caatgtccag aattgaccta cacggtccca tcgctctcca aaggcatggt agtacacgat
     9361 gatctgatag atatttgctg tctcataact atttccttct gtttcatttg aattgaatcc
     9421 actctttgtt ttctccgcat aatagtaatc ataataccct tgcttcatca gaatggttgt
     9481 tgtataatac cctttttcag gttcgtattt catcggttga gcatcttcgc ttgtccccca
     9541 ttggttaaac tgacccaaca cataaacagg attggacaat cgacccggtg atttgagata
     9601 gaaatgaacc agaaaataat cgttttccat ttctgaatcc ggacggtcaa gattctggat
     9661 aagatatgag ccccccaaat ccggatagga tgaatgttcg gaccgacctt cgagattttc
     9721 tgtccgtaaa aaaacctctg tacggtcatt gaatctttcc agattttcta ttttataatt
     9781 cggtcgcctt acactacgga tatcgagata gcggaattct ttgcctcccg gaaatgatgt
     9841 ttccttgata tagtcatatg tcatgcgatc gttgcggaca aatcgggggc ccaataaagg
     9901 cctgttgttc cagatgccat tcttgagtat ggctatctta acctggttaa gaggatcctg
     9961 aattctttgt gacgtagtta gacttaggtc gatgcgctgg tcttcacgca tcttaccggc
    10021 ttcgagagaa ctggctaatg tcgctgccac cccaaagagt tgctcactta ctacaaatct
    10081 ccgtgtaaga acaggcatat tatcatcatc gcgatatatg tgtatcagat aatttcccga
    10141 cttggtgaca cttatcaagt catttggaaa tactaaacta taggttgtgt acactacccg
    10201 ggtattgatg gaataggcat attctcggat ttcctgatca ttgaaccctt caagatattc
    10261 caattcgttg agttgactcg gattccagtt agcatcacat tgtacgatac ggtatttgta
    10321 atagacaaag tctccgttga ggtcatcaaa cttaagttcc aacggccggt cactgttgag
    10381 gtaggtcatt ggaaagccta cggtggatcc ataaggcctt aatataaccg tcttgatttt
    10441 atcatcgtaa ttgtaatcat aattgaccag ttctgttggt tgtgtatagc ctgatgtccg
    10501 ggccagaatc cccaaaagta aaaggcacca aactctgatc tctgtcatac tcgttgtatt
    10561 tgattattca aaagtaatac gaatgcgata tttattatag ttaattcaca tgaaacaact
    10621 ccggttgccg ataaataaac gtaaaattca ggaaaaacca gtcttttgcc aatttattgg
    10681 cgaattgata acaaattatc tgcctgttga gaatctaatc ttttatattt cggtacctgc
    10741 ccaccagttt tgtcatttcc cggaattaga tcttttgtca gaatcccgac ttaggccgct
    10801 tgcagatctg tttttataat attttcaaaa gacttattgt tcttgcacat gggcaaagca
    10861 ggtattgatt ggaggtcaag gaaggcttga atgccggtcg cctatagaca acacctccac
    10921 cggatatttt gaaagatcct gtaatcaatc aggaatttca gtttctccaa gttcatcctg
    10981 atctttctca agcctaccat atcaaactcg tgccggcaac ttccgccaat tgcctgagag
    11041 caagcaaact caattttgta cgaaaattgt taaaagcctt cctcttgtgc ataaattatt
    11101 agaaatcagg gacccgatag gctatatcca attttatata aagcgaacca actcttttta
    11161 ttaaaaaccg tttatcaaaa gtcaatgata ctaaggaatt gaatcccgga caatatatta
    11221 tcctgtatga tggcgattgt ggcttttgca atcagtcggt catgtttatc atccgaaacg
    11281 ataggcaaaa gaattttttg ttcgcctccc tgcagtctga aattgccggt gacctactca
    11341 aaccattcaa catcagacca tcgctcaatt ctatgatatt attgaaagag ggtcaatatt
    11401 atgaaaaaag tggcgcagca ctggaaatag tacaacatct gtcgggtgta tggccgttgc
    11461 tctcattact gaagatcatt ccgcgtccaa tcagagatgg cgtttattgc tggattgcaa
    11521 ggcatagaca ccgattttta tccgacactg catgcaccct ccctatacct gatgtcgagt
    11581 cgagattcct gaagtagcgt tacatttaca ctaggaggga gtcacatgga aacagagtcg
    11641 ccggtaggga tgttgttctc cgggactaaa tcggcagact gatagacatt taacgggtaa
    11701 aagggatcgc aaaaagagat cctactttcg gtgtgctttc aatttttagt tttaacttat
    11761 gtacctgtaa cactgcatcg atcagcgaaa gtccgatgcc ataacccttg acatgcttag
    11821 tattgatgtt tctgtagaaa ggctggaaga tcatctccat atcctctttg gcaatgccgg
    11881 cacccttatc gcggaaactg agctccaaac cccggtccgt agaatttatc ctgatatcga
    11941 cgtgtttgtc atcgctgtat ttacagcaat tatccataat attgatgaag gccagtttca
    12001 acagcgcctc atttccctct acggtaagtt tttcttgaga gtccggttgg ccctctattt
    12061 ggaagctcac tgaatattcc ggatgagaac gaagtaactt ggactggcat tccaggatgg
    12121 cctcatccaa ctgaaaattt tgaatatcga gatgaaaacc ttctgatttg attttggcca
    12181 tctgcaagag attctccgta atgtaggcaa tatcctgaac atcgtcacgt aaagatgtca
    12241 gggtagcacg atactcatcc accgtgcgat ccttctgatt gagcaataca tcgacttggg
    12301 agaggacgat ggaaattggg ttttttaact catgagaaac attactgata aacatttttt
    12361 ggaaggtcac cgaactttgt attcttgaaa gcaggttatt gatggactgt atgagatgtt
    12421 ctatctcgtc cttggtgtaa gagcgcttca gacggctgtc gagcttggac atgtcaagtt
    12481 ggtccatttg gtggacgatg gaaaaaagtg gattcagtgc ctggcgtgtg tagaagaatc
    12541 cggatacacc gacaacaacc aaacctaata taatagccag tgagatgatt cgtttcaggt
    12601 ccatcaactc cttagagttg aatactgatg aggcgactac ataatatttt ctaccgctca
    12661 tagatgtgta aggcagccca aaatagtaca tgtcaccggt atggtaggag aattcccgct
    12721 tcgatatcgg ccggatgtca aatggaagga caaaagcaga cttcctgttg aaagtaaata
    12781 ccttttcata attgttgttg aaaatgatga cattgtcact catgggtagg ttttcgctat
    12841 ccttgtgggg agtgatatct tccagcttat cgaggtctcc tttgtgcagc accatctcgg
    12901 ccgtcatgtg ggctttggtc tttaggttgt tatagtattc attgtaggtg atgtcctgaa
    12961 atcgtcggta cacaaaaaac atggacaaaa tcaggatggt tgccaccacc acgaagtact
    13021 gaagcgtgag cttgactctg attttcattg ctcttcatgt tggattttga aaatataacc
    13081 aatacgtgga agggtgtgaa tgagtttttc gctgaaatcc ttatcgattt tgttccgcag
    13141 gaaattgata tatacttcca gaacgttggt actggtctca aaatcaaagt cccagatgtt
    13201 ggaaagcaat tcctgcttgg tgatcgtcgt gttcgggtgt gtcatgaagt agtgtaacaa
    13261 ttcaaattct tttggtgtca ggaatatatt gatgtcctgg cgacgtactt tcttggtggc
    13321 cggattgatg gtgatatcgg cataggtgat ttctgattgc gaaggggcgg ccactttagg
    13381 cagacgcttt attagggcgc gtatccttgc caccaattcc ctaaagtcga aaggctttgt
    13441 caaataatca tcagcacctg ccgcataaac ctctaatttt tcttcgacca tattgagagc
    13501 tgtcagcatg agcacaggcg tatgaatatt gttctgcctc aatgctttta acatttgaag
    13561 acccgtgctt tgaggcatta caatgtctga cactatgacc ttgtattgat tggcaaaggc
    13621 gagtttcttt ccttctattc cattgtaggc cacatctacg gcaaaaccct gctcttctag
    13681 tcctttttta agggaagagt tcagcttttt ctcgtcctct acgataagaa tcttcatgca
    13741 atgctttgcc cgggctaatt gttgaaaata tatcggcaaa gataataaaa aaatgatttg
    13801 ctactcatta tgttaaaaat atttaatgtg taaatgaata tgtttcagtc aaaacaataa
    13861 tttacccggg gttatatatc ttcatttatt ttaacaaaga caaaagcttt tccattagta
    13921 tttaaggacg agttgttact ttcaaattat atgggaattt tttggaattt attcaaaatg
    13981 attttcctgc ccttgtggtg aatttatttt ggttttttta aaaccaagaa ctccgggtat
    14041 caataaattt ttttcatctt tcgaagatta gttactatct tcatatctct atttataaat
    14101 ttactacctt aaatgatagc tgactctatg tctgtacggc ttagtgtttc gaagttaatt
    14161 gcgttgtggg ccttttcaga aagcgcgttg ggtggtctct tacacgccac caacatgccg
    14221 ttttccggtt tgctgttggg cggaattgca gtcacagtga tatgctgcat cggatttacc
    14281 gcggtgcgtc ccggtgccgc catactttac ggcaccttgg ttgcagttag cgtaaaattt
    14341 gcccttagtc cacacagtcc tgttacttcc tatatcgcag tcggttttca gggaattgtt
    14401 ggggcattga tgttttcatt ccagcgcaga tcacttccgg tatatatatt ttacggaggt
    14461 attgccttgg ccgaatcagc cctgcaaaaa gtcatcaccc ttacggttat ctatggtaat
    14521 gccttgtggg attctttgaa tgtagcggca caacagattg ccaaatcttt gcatatggac
    14581 agtagcctga gctatgccgt cattttactt tgcatatatt caatggttta ttttgtttgg
    14641 ggttgcctga ttggttattg ggcacataaa attccgcatg aaattaaaaa cattgacaaa
    14701 tccgagatca ttagcatcga cacttcaggc aagttgttta ataagaagaa aaggaagata
    14761 cgttacctgg tgtggatgcc tatggtattg attttgtctg tttcggtaat tcttatgcgg
    14821 caatcaggct taagtgtttt gcagcttatc ttacgtccct ttattgtagt catggtatgg
    14881 tggtttgtca tcaatccctt cctcaaggcg atgttggcgc gctttttaaa tggacgcagt
    14941 aacaatatgg cgcgcatcat cgacagcatc aggcaggata ttcccaggtt gagaaaactt
    15001 tcgttccaga ctgccgatta tgtgacccgc cattaccgag gggtcagcag agtgcgccgt
    15061 ttcataacca tcctaatcat cctggctagt gaagaaaaaa gtaatcttac tggataatct
    15121 ctgggtcatt accggtgagg tgaatagtgg gaagacgact tccctgctcc aatggaccag
    15181 gggcagaaaa gtagccggct tcctgacccc tgatatcgat ggacaacgta ggctgatgga
    15241 tctttctaca tcagagattc ttccatttga ggctaatgat gatgacgaag acgtccaaaa
    15301 agttggcagg ttttcgtttt tccaatcggc atttgactat ggcaatagcc tgatcatcca
    15361 ttcactacag gcagatcatc catatttcat catcgatgaa ttcggcaaac ttgaaatcaa
    15421 cggccagggc tttgcatacg gcatcgacac gttgatgggg ggcatccgga cttcacaatc
    15481 tccaactatc tacatcatcg tcatccggga gagcctgttg gatgggtttt tggagcgata
    15541 tggcttatcg gattgagtga tgggatatga ctcctttggc ccaatgacta tattgggaat
    15601 atcggtgtat acaatgttcg gtttgtgtta tgcacgttga tagtatgacc ttcttttatg
    15661 ccatcttatc cgaatgacca taaagtcagg atataaccgt gtcttccccc cggaataata
    15721 tttgaataaa gaatattgta caccctacaa ttcaaaaaga tatgccgggt attgacttcc
    15781 tgccacctgt cgccaataat ggcttcgggt atgttttgat gcatacggaa aggttaatca
    15841 acggatgctg cagtccgttc atttagtaat ttccggctgg tctgatatac tatcttgaga
    15901 ttctggtcgg tatccagttt cttattgagc tgaagaagtc gtaatcagac caatcccaaa
    15961 ctatcctatt cttggaatca cccttgatct tatctaaaaa tgcttttggt aaaatcacag
    16021 gtctttgggc atggtgcact accttttatt atgcgttgta caattataaa agaagtgctg
    16081 tctccccttg ctcctttttc tgccttaccg acaaatcctg taaagaatga tttagttggg
    16141 ctcgcatcaa gtctaaatgt atcaatgccg aaatatattc tgactttatc atatttgctg
    16201 gttgtatcgt tgaacatttg ttgcaatgcc tgaaactcat cccgggtcac atctacgaaa
    16261 ctaaaaccgg cattttgttg cgtaggtcct cgataagtat gaatagctgt tcttgcagtt
    16321 gctctatcca cttccgagaa ttgaattact tgtcctgtat cctgaggaga gaccggatcc
    16381 gatggtcctt ctttttgaga tctgttacac ccaatgaatg aaagggttaa aagaaatgaa
    16441 attaaaaggg cagaaaaggt taatccttgt ttaattaata atttcatggc ttgcgtattt
    16501 aattttatgt aagtataagc aggcaaattt aacaaatgat ttatatttgt ttataaaaat
    16561 atttatacag taaagcggct gtgtcacaaa aggaaattat tttgaagttt aattgttgtt
    16621 atagaatgtc tacagacccg tttcttgcta tttgccggac atttcttttc cttacaatca
    16681 ttatgttggg catgttgggg tgcaagcgtg gtagcgaaga aaggcataat gacagtaaga
    16741 tagtgaccaa tgacttcggt caaggcattc cggggattct ttacagggca aaggtacagt
    16801 tggattcctt tggttttgtc aataaagcgg attctttgct ggaactcgcc atcaatgaag
    16861 cggtcaaggc aggaaaaaat gatcaaatag ccatggcata cacttttgcc ctgaaatatt
    16921 cctcctccaa accggtaaat gaagcccagg cgctggaatt ctacaacacg ggaaagtacc
    16981 acgccggcct gtcgggtagc atttctctcc tcaacgagct gaactttgcc atggcacatt
    17041 actacaccaa gagaggcaat acgggtcaaa gtgaaaaata tctcaaccag atttccttct
    17101 ttggagggag caattttgaa ctttatttga aaaaggaaat ggctgaagcg gccaattttg
    17161 gagtcagaga taagcctttc gaacagctga agtctttact tgacgctatg tataaggctt
    17221 acgaattggg tcatgattcc atttatcagg catgcctgct taacatttcc gacttttatt
    17281 attatgaaca acaatacaac aaagcactgg actatatcga tcagttgcag aacaccatag
    17341 ccgcaaaggg aatacaaact gactccaacc gaatatactt cctcatggcg aataaattgc
    17401 cgatattcaa caagcaattt gaccagaatg caacaatcag gctggcagaa tccatttccc
    17461 actatgccag gaaacatggc tatcaattgc tggaagagat agctcagggc caggtacgga
    17521 ctgccttgat tagcagtggt gactttaagc gcgtggaaga ctattacaga gcttaccctg
    17581 tctacttgtc caggcttgag cacgaaaagc cttctatcta ttacaggatg ctgtcctata
    17641 ttgagatggc aaagggtaaa aaagacagcg cggtggccta ttttgacaag gcgggtgagt
    17701 ttttggatgg ggaaaatgaa tattatgtag ctaattacca tttcagaaaa ggggagttga
    17761 tggaaaagct cggtcagcga tcagaaagta tttcaagcta tagcaaggca ctcaaaatgc
    17821 tcaacaatag tcacgacctg acactccaga atactttgat cgacaaattg attccgtatt
    17881 acaaggagat taatgacccg agatccattg tcggtttgtt ggaaaagaaa gattcgctga
    17941 acatgaaact gattcaaatc aataaagagc aaaatatccg ccagctggaa ttgagtaata
    18001 tcctctccgt atacgaattg aagcatcaaa aggcggctga agagctcaac agaaagcagg
    18061 tgatccagtt gcagattgta gccttgttca ttttactggt attcatgagc cttttggtga
    18121 tggccaatct gaaacttccc ggctggtggc ttagatcaat gggctatatt tccattatta
    18181 tcttatttga atttattatt ctgatggtag acaggagact gcacgatatg ttgcacggaa
    18241 gtgtaatcaa attgttggtc attaaagtca tactgatatc ctggatctta cctttgcacc
    18301 attgggctga aaagaaaatt gtcgcttttc ttttgcaaaa ggacaggttc agaggtctta
    18361 aggatttcct gtccaggtta tttcaatcct ttggaaaaaa agaagttgaa gaatcgtgac
    18421 ttccgattgt cgttgtacat catgaatgga ttgcccctgc aacttgcatg ttgacaggat
    18481 tacttcttat gcctgatgac tcgtcttgtg ttggaacctt tgattgtatt tgttggtctg
    18541 tgtttcttga tcgatctgat taacaacaag caatgccact caatgagcag gaaccgggat
    18601 acctctcccg agatgaaaat caacaccgaa aacaagaaaa gaacattcct gactccagat
    18661 acgggatcga tgaggtcact gtaaaaaggc atgataacca aggcttcaat ggccatcagg
    18721 atggtggtag taatcactcc ggcaatgaaa aatacccggg tgggaagaac cagtttacga
    18781 ctgcggttca tcatgatttc cttgaagaaa tacctgacat ttggctcatc cggattcatg
    18841 gacaaaagct gccggatgat gctgagtgaa tcctcaaccc tgcctgcttc gttcagtgcg
    18901 acagcttttt tcattaaaag gttgaaataa atatcgacac caaacaacct tgaaaagccc
    18961 aactctattg cggtttcgat agcatcatcg accaggaaaa gtacttcttt gaatttaccc
    19021 atctcaaaca gtgcctcgat gtaacacaca taaatctcaa aacggtgtct tgcatccaaa
    19081 ttttcgattt tgttgcggtg gtcttcatag aaatgaatca cctctcgata tgatccggag
    19141 ccaatcgtca aaaaacggtc aaagaccgaa gggtcatata tcgaagtaaa gtccatttta
    19201 cctcaacctg tataaaaacg gtctgctggg agatacatcc aactgaatat ttaggagatg
    19261 taaatttaac aaataaagtc catcttcaac aaaattttct gcaaaaatca attcagcaac
    19321 cgttgcgccc gcccgtaaat caatatccgg ataattccaa aatgccctgt aagcttctaa
    19381 tttaccctga ttttcctccc gatccaacga tggcaggtcg gttaagagat gctttatacc
    19441 acggctgacg atcaggtcga tagcatcacg actgaaataa ggcggattgg tatctgtgta
    19501 ttcagtcttt ctcttttccg gcccattggg caaagtcctt atgatcaagg cttcctcacc
    19561 aacagcctcc cacaattgcg ataattgttc tgcatgaatg accttgtcgc cattatctga
    19621 taattgagga aagatactca ccagctgtgc aacatggaag tccggcttgt agtattcatt
    19681 gatataatat tggtccctga cgatatggcc gacacattgg gtgtgcgtgc cattgccgtg
    19741 aggatggatg gtgacattat aaaaattggc gtgccctccc tgagctatac ttttgaccag
    19801 atcgccttct cgatgtgggg acgcatagaa gtatggagcc cggaaacaat tgacctgcgg
    19861 ataaccacta tgcaggatca ttgagatatc aatggggtgg tggagatcag ctgtatattt
    19921 ctgaccattt aaggttacgg acacttcgat attataagtt tcaacattca cgacattcgg
    19981 attcttgtat tgtcggatta cttgatcggt ctatcattta ggttacaaaa aggttatatc
    20041 ccggatagcc gcaatattaa ctgtacatat agtgatataa caacgcatgg aagaataaat
    20101 agttaacgtt tatttttaaa cataattcac ctaactttgg cgtttatttc gggatagata
    20161 gaaatacgga acagtatttt tctttctact ttaaaagcaa agaatggcaa agccggtatt
    20221 tattgtaatc gagggtattg atgggagcgg taaatctacc caaattgagc tgctccaaca
    20281 tagaatgtca gaagaaggga agcaggttgc gatcaccggt gaacctacgg atggctttat
    20341 cgggaagatc atccgggaca tcatgcgcgg caaggaagag accgacccgg cggtcgttgc
    20401 tgcattgtat gtagccgaca ggatggatca catcacccat ccggatgtgg gcatgcttgc
    20461 actgttggag aaaggataca atattattgc cagccggtat tatttttcaa gctatgcctt
    20521 tcaaggtgaa tatgtttccc tggagtggct catccatgcc aacagtatct gcaaaagccg
    20581 ccttaaagct gacctgaccg tttatatcaa catcgaccct gcaatatgtt ttgaacggat
    20641 taaaagtagt cggccaagcc ttgatatcta tgaaaatctt gaaaaaatga agcgtaccca
    20701 ccgcgaatat atgaaggctt ttgaggtggc aggaatggat gaaaatattg tgatggttga
    20761 cggcaccttg ccgatcgact tactgcatga agccatttgg tcaaaggtga aagaagtggc
    20821 tttctgatcg aaaacgtaga gaaaaaaatg tttgtcctgt aataacagca gttctcatca
    20881 atattattcc agctaatttc agcttaagaa agtaaccgaa tccacgaaca tccgcgttca
    20941 aatgaccggc tggtgcagcc tttccccgca ttgacggcag tacactgcat cgttgctatg
    21001 ctccttggtg ccacaatttt cacagaccag gttaatattg gtcttcttca tctccttgat
    21061 gatgttaccg cctatgattc cggtcggtac cgcaatgatg gaataaccga ggatcatgat
    21121 gaaggatgca agtatcttgc ctatcggagt gattggagtg atatcaccgt aacctacagt
    21181 cgtcagcgtg acgatggccc aataaatgct ctgtggtatg gaatgaaagt tcggattatg
    21241 ctggtactct gcgaggtaca ttaaagaacc aagcaggaaa gccagaatca atatgaagaa
    21301 gagaaaaatg gctatcttcc tgaaacttct gattaaagca aagaccagtt ctcgactttc
    21361 atccagaaac ttaaccatgc caaatatcct gaacacacgt aataatcgga aagaacggat
    21421 gatcatcaac atatgacttt ggggaagaaa atactcaaag tatgatggga gaattgctat
    21481 caggtcaagc atgccaaaag tactcttgac aaatcgcttt ttgaccttga cgcacgacaa
    21541 tcgcaacaaa tattccagcg tgaaaacaaa caggaaaaag cggtcagctt gtctgaaata
    21601 aaaagaatat tttgcactca gggactcaat actttctgcc agaatgataa agatattaat
    21661 caggatcatc aatgacagaa tgatgtcaaa cattttcccg gccttagtat ttgcctgaaa
    21721 aattataacg taaagtttat actgccagga gttcttgtca tcaggtctgt tttgactctg
    21781 gacaacatca attctatccg gtaatattct aaataagctc aaatcaaggt tgtttcaaaa
    21841 tcaaagtaaa ggaatattac aatgtcttca aatttataca aagatagaat ctttataatt
    21901 gcctgatata tgaatttttt gtaactattc accttaacat aacttaatca ggacatcttg
    21961 ataccaactt aaaaggaatc accatgatta atcaagggtc atgatcagag gtttttttat
    22021 tcatcattag caaacttgtt tgcctgcaac tgcttaatgc aaatgatgat tggatagatc
    22081 tttttgtgtt ttttatgctc tccgagaata aaaattgatc cgataaatct tttttttgat
    22141 ccggtgattt agagaagcta tttagcatct ttgcatagac ttttccagcc tgtaaaccct
    22201 ttttcccaaa tcccattttt tgcaaaggac caaacctttt gggtcaagat agtttaataa
    22261 tggataaata tctatctgat gtgaataatt gcgaatccgt actattaaat attacttgct
    22321 gcattggcat gaaatgaagg caggaagtag cttttaggga aatatgattt tttataaaaa
    22381 attatatatt attttaattt attatgtgta tttttgtgag attaaataaa taagcctgac
    22441 tgtctataac gtttttaaga ttgtaatgca aagaataaac ggatcgatta aagttagaaa
    22501 cactgatatt cagggttatt tgcctattcg atattctccc ctccttccat ttccaatatt
    22561 caatttatga ggatttttat tctttcaata atttgttgca tatcatttgt ctttgcttta
    22621 ccggctcaac aaggagtcta taatatggtt gatttctata gggagctttc ggttggacat
    22681 cctgttttgc cctttccgga aaatgttaag tttgaatcag ggctgaataa atacatcggg
    22741 gaaccaatgg ctctattggg tcaaagggcc cccggttcaa gatctggaca acaaaatgag
    22801 atgtccgccg tgacaatgac ggagattgag catttattca gtggcaggat tcaacgagga
    22861 gttgttcatt ctgccaatca gcctccggta gctgatgccg gtccggacac caccattgtt
    22921 ctgccggtcg attccgtcct tttgaatggc tcaggtaccg atccggatgg aaccattgta
    22981 agtttcgaat ggactccaga tccaaacaat ccgtcagccg ctactttcga tgatgcttct
    23041 ctgcccaatc cgatggtaag caatctcatt gaaggaattt atattttcac cctgacagta
    23101 accgatgatc agggagaaat cggttcggac caggtcacaa taaccgtaca ggctgctaat
    23161 caacctcctg ttgtcaatgc aggggatgat ttaacaatac acttgccgga aagttccgtg
    23221 caattgactg gaacagcgat tgatcctgat ggatctgtcg ttagtttttc ctggagtcag
    23281 gatccttcaa acccggcccc ggcagccttt tcgaattttt cgattcctaa ccctgtggtc
    23341 agcggtttga tcgaaggtgt atatagtttt acactcactg ctgtcgacaa cgaaggtgcg
    23401 accggccagg acaatgtcct ggtgaacgtc cttccgccca tcaatctgcc tcctgatgcc
    23461 catgtcggtt ttgacctgcg agtcattctc cccactgatt ccactattct tcatggcagt
    23521 ggcaccgatc cggaaggtgc tccgatcact tttttgtgga aacaaatcgg cgatttgcct
    23581 tcgattgcaa cgatttccga ttcggcgagc tcagatccgg tagtgagtga tctcatcctt
    23641 gggatataca ccttccgatt gattgtgacg gatgacaaag gactgtccga ctcggtagaa
    23701 atgaatgtca tagtggatat cgtgggcaat atcaaaccta tagctaatgc tggcgaggac
    23761 gctaccatat tggtaccgca gaccagctac cagctcgatg gcagtggctc tgattctgat
    23821 ggattcatca tcggttacaa ctgggaacag attggtacct tgcccaacac agcaatcatt
    23881 tccaatccgg gtgttcccaa tccgattgtt tcaggactta tcaagggagc ctatactttc
    23941 cgattgacgg ttacggacaa tgaaggggga aagggcatga atgatatggt tctcatcgtg
    24001 tttgatacct tcaatattgc tcccatcgcc aatgcaggcc ccgatgccac cattgccctt
    24061 cctcaggaca cttatcaact aagcggaagc ggtaccgacg ctgatggcat aataatttca
    24121 taccattggc aacaggtcgg caacacccct tcccaggcgg ttttttccaa tacagatatt
    24181 cctaatcccg ttgtgtcagg attgatttac ggacactaca cctttaggct gactgttaag
    24241 gacgattatg gtcttacggc ttcggacgac attgtgcttt ttgtaagaga tagcttcaac
    24301 catattccgg ttgccaatgc aggtgaaaac attcgactga ccttacccga caatagtgtc
    24361 caggtccatg gtaccgggta tgatgatgat ggaatcatcg ttggttatga atggcaacaa
    24421 gtaggtaaca aaccaagtaa agccgtaatt tccaatccgg atatagccga cccgattatc
    24481 ggaggttttc tgtacggcac ttataccttt gtatttacag ttcgggacaa tgaattcgct
    24541 accggatcgg acacgctgct gattgtggtg gacacggttc cattaatagc gcccatcgct
    24601 gatgcaggtg ctgatatttc cgtcaatctg cctcaggaca gtgttcagtt gcatggcagc
    24661 ggttcggatg aggacggcac cattgtaggc tatacctggt atcaattagg gacacaaccc
    24721 tctccggcgg ctttttcaga tacaatgatc gctgatccta ttgtaaaaaa tctggtcgaa
    24781 ggcacctatc attttgtatt gaaggttacc gacaatgaca acttaatgga taccgatacg
    24841 gtaagggttg tcgtcagcaa ggctaagaat attcccccgg tggccaatgc cggcgaggat
    24901 ataacgatca agctgcccat tgatgcaacg accctgcatg gtagcggaag tgatgacgat
    24961 ggtacagtga cagactttct ttgggagcag gtaggtacta ctcctgatat tgcaaccatc
    25021 atcgggaata attctcctga accaactgta tccaatctct tcaagggaac gtataccttt
    25081 agactgatag ttacagataa cgagggtgcc aaggataccg atgaggtcaa ggtctttgtt
    25141 ttggacaaac tgtctaccca taccggggat gtttcttcgg ccatctatcc gaaccctgcc
    25201 aaaggcggca tcttccatat taacccgggc gccaaattaa agcctggtac atcggctcgc
    25261 atatttgaca tagatgggcg ggagatgatg cgctttgtcg taggccggga agatagctcc
    25321 tttcaatgta atcaatgcgt atccggtgtt tttgttattc ggtttgataa cgggatgagt
    25381 gccgctttgg tcgttatgcc ttaagaagtg tattgtaaag ttgacggcgt gtaacctaac
    25441 cacagagcac caaaagaatc cgggtatttg ttctttgtgc cggcctgttt caatgttata
    25501 ccaagatgat ttatataata gtccataaat ttttggtatt agaggctaaa tggctataat
    25561 attttcaaat acctgaaaat caacgtctgt gtaattaagt cccggaatag ttttcagtca
    25621 atgttcatga tgcatctcta attgatcaag ctccatataa ggggcacatc tttcaaagaa
    25681 acatgatcag agaaattatt caggtaaata ttgatagaaa attgaggtag tagatgtgac
    25741 tggcagcaat ttataggagg ctgtgacgat gcttccattt cttgttgcat tgaaattccg
    25801 gtgcgatgga gggttcacag tctactttgg ttttttgatt ttcaattcat gatatcaatt
    25861 aatcgaagta atgaaatata ccggtagaga atgagctttt cccggatgtg agaataatgc
    25921 agtggtcgga cgggtcaata aaatgattga tggccttccc caatttttct tacttttgca
    25981 gcgttgattt gtaagtgatg ttaaccatac aggatattca gggttcagac aatgtgaaga
    26041 aagagattac aacactgatt tcaagtggtc agtttccgca tacaaccttg tttataggcc
    26101 ccgaaggcag tgaaaaatta aatctggcat acgcaactgc aagactgctg atgtgtgaaa
    26161 acaacaagaa tggcgtcata tgtggacaat gcaattcgtg ccgtaaatct gccaatttta
    26221 cccatccgga tattttcttt tcattcccat ttgtgaagga gggaagcggg gatttccaat
    26281 ccgatgactt cctgcaggca tggaaagagg cattccggga aaatccttat atgaatgtat
    26341 atgaatggtc actgaaacag gatgccggca ataaacagcc caatatcacc gcattcgaat
    26401 gtatacaaat acagcgtaaa ctacagaaga agccatatga atccgaccgg aaagtcttac
    26461 tcctgtggat gcccgaatat ctgggtaagg agagcaatcg cctgttgaag atactcgaag
    26521 aaccacccgg tgataccttt atcattttgg tagctgaaga ccaggaacgc ttgttggcta
    26581 caatactttc gcgatgtcaa ctcttcaagg ttgagccatt cgacctgcaa ggtgttcaga
    26641 gaatattgga aaatcatttt ggcaaatcga aggaggaagc cactatggca gcatatctgt
    26701 cccgaggaag catggtagaa gcactgtcgc tcttcagcga aggtaacctg gagaaaaacg
    26761 gtgaattcgt gacctggctc cgcctttgct attccttcaa tcatgtggaa atcatacgat
    26821 ggatagaaca cttcgctaca tatacaaggg aagttcaaaa atatattttt aaatttggca
    26881 ttcatttcgt caatgaaatg ctgaaaatca aatacaatta tggggtggac aacatcagcc
    26941 tgccaaagga aatggccgat gtagcagccc gtctttgtcc actgttgagt tttgaaagca
    27001 ttgaagagct ggtcaacaca ctggacagag aaattttttt aacggagaga aattgcaaca
    27061 cgaagatgca gttgatatat acatcgggtt atatacaccg attgttcaac ggaaagcggt
    27121 tcgcagcgtg atttctcatt aaaatatatt aatcatgggt tgcaatagtt gcagtaccga
    27181 agatggcaag cccaaaggct gtaggtcaaa tggggtttgt ggcacttcgg gatgtaataa
    27241 actaaataca tttgactggc ttactacaat ggaactggaa gaccccacag aggtcaatct
    27301 ggtcgaagta agtttcagaa aaggtgcgca taagtcattt ttcagacttc aggatgcgta
    27361 tcaggctgag attggcgatg atgtcgtagt agatgcggat ggaggttatg atataggcag
    27421 gatttcattg aaaggagccc tggttccgat tcagatgaag aagaaatcag tagatcctaa
    27481 ttctgccctt agatttgtca tcagaacggc cagtcagaac gacatggcaa aacttgagca
    27541 agcgataagt atg
//
LOCUS       DAOQQN010000002        25577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_578_length_25577_cov_5.594938, whole genome shotgun sequence.
ACCESSION   DAOQQN010000002 DAOQQN010000000
VERSION     DAOQQN010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 25577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25577
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_578_length_25577_cov_5.594938"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1218
                     /locus_tag="PLG24_00095"
     CDS             124..1218
                     /locus_tag="PLG24_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate delta-isomerase"
                     /protein_id="HMZ23306.1"
                     /translation="MVQTQKQPMDPNPEVLENDDLNADIRKQDHIDLAFKSATAAAMN
                     DSRFLYEPLLGVHSEWQEEMVIFGKTFRLPFWVSSMTGGTEKAARINRVLATVAGKYG
                     FGMGLGSCRSLLYNDEYLSDFAVRKYLGDDAVLFANLGIAQVQQLIDEGKTSMIKTLL
                     DKLEADGLIIHVNPLQEWLQPEGDRYTKRPLDTIKALLENSDYKIIVKEVGQGMGPQS
                     LEALLALPIEGIEFAAFGGTNFAKLELMRGSEEKRQEIMPLAKIGHTAEEMVQLVNHL
                     GKRLGDNMRCRQFIISGGIQNFLDIHYLRSSLNYPSLAGQASKMLRYAQIGEGALDEY
                     IDTQLKGLAFAQNFLRIREDANTYHLNKKL"
     gene            1231..2568
                     /locus_tag="PLG24_00100"
     CDS             1231..2568
                     /locus_tag="PLG24_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HMZ23307.1"
                     /translation="MNSEGTGKTVSGFSKLSKIGKLKWIVNNFFKDPEAVTKEMKSYW
                     FQDEQDQKIIDSLSENTISNFVLPFSVAPNFIINGREYAIPMVIEESSVVAAASHAAK
                     YWSQRGGIHARVVSTIKEGSVYFFWDGDISLLMTGLPELDKLIREDCRELTAKMESRG
                     GGIVDIGFVDMGHKEPGYKKLNIRFETCDSMGANFINTILEEASSVMKGWFEAKGYDS
                     PDERRLEVIMSILSNYTPQCLVEASVECPVEELTNIPEMTPQAFARRFYKAVRIATVD
                     PSRAVTHNKGIFNGIDAVVLATGNDFRAVEACGHAFAARSGHYQSLSTCTINDGIFSF
                     TLLIPIALGTVGGLTRLHPIARRSLELLGNPGAEELMQIVAATGLAQNFAAVRSLVTT
                     GIQKGHMKMHLQNILWQMKATPQEQELLIEYFEDKIISYSSVRAQLEKIRSTL"
     gene            2569..3522
                     /locus_tag="PLG24_00105"
     CDS             2569..3522
                     /locus_tag="PLG24_00105"
                     /inference="COORDINATES: protein motif:HMM:NF040656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYDIA family GHMP kinase"
                     /protein_id="HMZ23308.1"
                     /translation="MNISKDEIPGIHYNARGKLMITGEYLALKGARCLAWPTHALHHL
                     EVHTLAGQPGTLIWNASDKDGKPWFDALFSTSERVETISSSDFSAADYLGRMLNAAIA
                     LKGKNILEHASFHVETKIEWPMEWGMGSSSSLLVNIASWFGVNPFTLQQKTIGGSGYD
                     IACGLSDEVIVFQNAGKLLANKVHRPEILVQYAGFAYLGKKADTASAITDFSSRTEGM
                     DLTAKIHLIDRLTYHILDAKTVHHAIKAIEIHEEVIGSLLDVEPIKLRLYKDFDGAVK
                     SLGAWGGDFIMFVGSKPLEEFRVSTEEKYGIKLYSAAELLV"
     gene            3541..4263
                     /locus_tag="PLG24_00110"
     CDS             3541..4263
                     /locus_tag="PLG24_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ23309.1"
                     /translation="MPKWIEATVTKIVTENDRVRRLYIDPRTEETFTAIPGQFITFDL
                     PVGEKRLQRWKSYSIANQITTGEIELCIAKQEGGLGSTYLCEEVKVGDVLKFKGPDGM
                     FYLPDPLPPTVVMICTGTGIAPFRAMLQAALNHDKYKNTQFHLIFGTRYETDILYKED
                     LEAFSKYPNFKLDIALSRHIGDGNYHKGYVHSIYLNHYNSEKTDALFMLCGWQEMVDE
                     ATDILQNAMLMEKTRIMVELYG"
     gene            complement(4291..4758)
                     /gene="rfaE2"
                     /locus_tag="PLG24_00115"
     CDS             complement(4291..4758)
                     /gene="rfaE2"
                     /locus_tag="PLG24_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HMZ23310.1"
                     /translation="MKNKVLNREEVILQARTWQQQGLEVIFTNGCFDILHPGHVRYLA
                     QSRTLGDRLIVALNDDDSVRRLKGESRPINTLADRAEVVAALCCVDAVTSFSEDTPYD
                     LISALIPDVITKGGDYMPDNVVGADIVEEAGGKVVIIDFQEGYSTTSILKKSK"
     gene            complement(4748..5839)
                     /locus_tag="PLG24_00120"
     CDS             complement(4748..5839)
                     /locus_tag="PLG24_00120"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HMZ23311.1"
                     /translation="MQRKKFIVDFLKFLLFLAIGAGILFWVYISQSRAFQEQCAIDGI
                     PEEQCSLIRKLIADFRSVNILWLVAVIVAFQLSNVVRALRWQMICKPLGYKIKLGNAF
                     WTIMLSYFANLGLSRAGEVIRAGSLARYEKIKLDNVVGTVALDRFLDLVTMSIAILIA
                     FFFQYEEIQSYLASNMRDFDFAAFSRGWIFQTFVTGIVLFFLLVILFRNRIRQSLIYL
                     KIKNFTKGVIAGMKTIRKIERPFLFAFYSILIWVFYFLMTYFAFFSFPPTASLGIQAA
                     LLVFVFGSFGILIPSPGGMGTYHALVVAALAIYGIRGDDAFSYANIVFFNIQILTTIL
                     FGVLSLIFLPINNKHYIPHHILISDGHEK"
     gene            complement(5842..6189)
                     /locus_tag="PLG24_00125"
     CDS             complement(5842..6189)
                     /locus_tag="PLG24_00125"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HMZ23312.1"
                     /translation="MLIQVFKSKIHRATVTEANLNYVGSITIDEDLMEAANLYEGERV
                     QVVNNNNGERLETYVIRGERGSGDICLNGAAARKVEVGDTVIIIAYGFMTPEEARSFK
                     PAAVFPDSNNKLV"
     gene            complement(6303..7169)
                     /locus_tag="PLG24_00130"
     CDS             complement(6303..7169)
                     /locus_tag="PLG24_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ23313.1"
                     /translation="MRLFHFLLLASLIITGSSCKTGKKLKAAQEQYKTLSDQYVALER
                     KLIDCETAVNEADKRKRVSDKEIEELNKQIVFLRENNTAVLGQLKDLSVITNSQAESI
                     KKSLENIGAKDNYIQTIQQAAARKDSLNLSLVMNLKGVLGNLNDQDVEVKVDKGIVMI
                     SLSDKMLFKSGSYDISENAGAVLAKVAQVLNNQPDLEILIEGHTDNVPISKPGIKDNW
                     DLSALRATSIARALQTRFGIDPKRITAGGKSEFSPLTTNDTAEGRSVNRRTRIIIMPQ
                     LDQFFKLLERKI"
     gene            complement(7777..9186)
                     /locus_tag="PLG24_00135"
     CDS             complement(7777..9186)
                     /locus_tag="PLG24_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ23314.1"
                     /translation="MDGNLNTNNNSVPNIVSEYEAMSRNGGLSLLHEKDFIELILYFE
                     KELMLDKALEITDLAIERFNFLPDLYLKKAQILLALNNTSDAFEAVDQALLLAPSENE
                     AKLLKANCLIINKEYDQAFNLLNDIMMVGSQKDRINTMLLRARIHEELKDYTQMFNQI
                     RNVLDLEPSNELAMEKLWFYMESQRSYHQGITYLNQLLDNDPYAYMAWYNLGQAYSCI
                     GEYENSLAAYEYSFLIHPSFELGYKDCADLAFQMCRYRFALKVYLEALDNIGPDSELF
                     ACIGECYYKLKEYRLARLNYRKSNKLDPYNDEVHYYLGLCYAAEHKYDIALQHYYKAL
                     KIEDSREEYFAATAEAFYKQGDADKADYYFKKATDLGGDIPEIWLSYVKFLYEIEEFD
                     RAFDVLEEADEYTYSSELLYYKVLVLIAQDDKEEACEVLKEALSEDFDNHVILYRLNP
                     ELKKDQEITSIINYFFSED"
     gene            complement(10033..11175)
                     /locus_tag="PLG24_00140"
     CDS             complement(10033..11175)
                     /locus_tag="PLG24_00140"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMZ23315.1"
                     /translation="MSELIADFSEDRKADLLKKWSLILSANPAKDEDFEGVEWSDEEE
                     TIDKALIWVYGDGGAGSSNRNFSVNDWLREVDFYFPNSVSTVLQKDAIKRYGLELLLK
                     DDAFLEHIVPDIHLAVSILKMKGLLPDVAKHKARLIVKELARKLTGLFAFQTTQAIGK
                     ALRSPIPNRRPKYNHINWVKTIYKNLETYQPAKRTIIPERLIGRQNRQKTIDTLFILV
                     DQSGSMHESLVYAAIYASIFSMIPALKTHLVLFDTEVVDLTGMEDDPVEVLFASQLGG
                     GTNIGKALAYAHKTCSNPDRTLMVLISDLDETSDEVTFMREAARVASTFNRFLTILAL
                     TKEGKGHWNVENANKFTELGIVCAASTPEQFPELCAREIVLSRSGS"
     gene            complement(11172..11417)
                     /locus_tag="PLG24_00145"
     CDS             complement(11172..11417)
                     /locus_tag="PLG24_00145"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HMZ23316.1"
                     /translation="MGDNWIWTIIVSGAAGYLGSLLFKGKGSGIIFNVILGVLGGAFG
                     SWLFNKLGITGIGNFLTSVVGAFILIWLFNGMSRNKG"
     gene            11553..11984
                     /locus_tag="PLG24_00150"
     CDS             11553..11984
                     /locus_tag="PLG24_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23317.1"
                     /translation="MNKNFRLAILFLSIIMMGGYGCRHSAPDWSSPVRIYDTLCSCKN
                     SSGHTVDAGLKKMLDEKNIQAENADQINTWISANATAFVAFVDSDFKNDPVFMSELNA
                     ARDTLEAHGARLETEGAFTELFSIKVKYPGCVLAMPYLITK"
     gene            12077..12262
                     /locus_tag="PLG24_00155"
     CDS             12077..12262
                     /locus_tag="PLG24_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23318.1"
                     /translation="MSNEVNIYEEIVKLNMRISCLESLLQHIIEINPYLEIPPKEEID
                     RIYSKAIQQVIDEVEAG"
     gene            complement(12566..13855)
                     /locus_tag="PLG24_00160"
     CDS             complement(12566..13855)
                     /locus_tag="PLG24_00160"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HMZ23319.1"
                     /translation="MVIFTFAIVAIITWSLLLIPYVLWLRQSTFFFLQRGYLLGSLVI
                     GLLNGVINILPQGKLNFGLVEMIKGDNFISDNLNSIISGITIGHGAVPETVWTTILAV
                     YYIVSCILLAILAYKVLTLYMMARGNSSTYLNGMRIVESPQRHTPFSFFHLIFLSKEN
                     RPDAESLVPIVRHESTHIKQWHSMDIILTEILFVFTWFFPVVFLFKKYLTLIHEYLAD
                     AEVLKTMDTRIYGELLLSQQMGASVSVAHHFAKSELKSRFRKMVQTPTSGRHRLIYLS
                     VLPFLLLSFIWAERIDLNAITAKVNNFNQQDDNPAISREIYITPEGQMRVVKKATGPD
                     KYIPQYPGGQEALGKFIASNLKVDGYVKTKTKDVRVRLNFDSNGKIELITYLVPVLPE
                     MELALNKVFNTMPAWIAPEIGGKPVRSEVVLPLQFNW"
     gene            complement(13861..14229)
                     /locus_tag="PLG24_00165"
     CDS             complement(13861..14229)
                     /locus_tag="PLG24_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HMZ23320.1"
                     /translation="MSDEERMLTKAEEEIMHHFWEKGQSSVSDIIAGMDDPKPPHSSV
                     STIVRILERKGFLGHEAIGRAYEYFPLVEKNEYSSRSLNKFVNDYFDGSFSGLVSFIA
                     KEQDLNMQEFNDLMDILNKK"
     gene            complement(14378..14899)
                     /locus_tag="PLG24_00170"
     CDS             complement(14378..14899)
                     /locus_tag="PLG24_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23321.1"
                     /translation="MNIRLKVLTVFCTLLITVSGIAQNSDESHYGHQHDHEHQEGHEH
                     HKNEIGVVVAPAYFTHTREFNFGLHLHYLRSLGESKFGVGAGYERIFGDHAHHFLGVV
                     AAYRPTGAFSLILSPGLAYEDAKHAGPQFGLHIEAVYEFDIKGIHIGPVFEFSYDADD
                     IHISPGIHIGIGF"
     gene            complement(15098..16153)
                     /locus_tag="PLG24_00175"
     CDS             complement(15098..16153)
                     /locus_tag="PLG24_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HMZ23322.1"
                     /translation="MIKKKILVCPLNWGLGHATRCVPIIQLLIQRGHEVHLASDGIAL
                     ELLRKEFPGLPAIQLPGYDIHYKGKNLLRTVAAQLPRILKTVILEHRFVQAYVKQNSM
                     DVILSDNRFGCYSGDCVNIFITHQLRILTPLRLMSAVAGMANRFLIRHFDRIWVPDFP
                     PPQNLSGYMSSSGSLHPVYIGPLSRLQKLSKPIIRDFIVILSGPEPARSDFEQIIISQ
                     ASLTPYRYMLVRGVEDQTPPPDYSGSGISWVNNMTTEELSYAIAESRRVISRTGYTTV
                     MDLMALGASGILVPTPGQPEQEYLAQRLKQQDRFILSSQEKFDLRFLTGQSLDDDRTF
                     EPISHNLTEKAVIESGL"
     gene            complement(16259..17170)
                     /locus_tag="PLG24_00180"
     CDS             complement(16259..17170)
                     /locus_tag="PLG24_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23323.1"
                     /translation="MRSMILVITILSFAVAASGQDIMKIGERTNKKIQRKVESGINRG
                     IDRTINDAEKDIRNSMKSKPQKDSGKKTGDSKTKEPGQTGVTEKYDFKPMSKSVLSFN
                     ANNMKDDNQTTESFNQVIELVQLDGFKGKWIKLLPNSAYCPIMSQAINGDWAIEMNLI
                     PQVGESNKSDINFSIALVGENDYNRLFSDSGLDAHEMDSLYNYVMVQINKPASKDELN
                     ILTKTRMSGTKPLVRKKKYIPVNSEVNRNTFAISNTGSITKVYYCNRKITSFQNKSQD
                     AMKIILFVIDDSGESALYLQDINITSK"
     gene            complement(17219..18586)
                     /locus_tag="PLG24_00185"
     CDS             complement(17219..18586)
                     /locus_tag="PLG24_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ23324.1"
                     /translation="MRSFELVFDPNNDNRSPNAVTLSKEFNWLANFITSQFAKYFGQD
                     EGVKDVVACDLQKDNSTYAEFTRYYNLNMEERVVLLMAMAPHVCPQLLDAFFVKNSTY
                     ARPFSEFGGLKGLHHVGFLPTGETAVFLLSLNDIADRFRIMRYFAPEHPFIKHRILNL
                     EKDGNSEPMLSGKLSISKEFLSILTEDKRFDPRFSSDFPANKLETKLAWDDLILEEYT
                     LHQVEEIIKWVRLENLILENTDLNKYINPGYRALFYGPPGTGKTLTASLIGKQVGLTV
                     YRIDLSKVVSKYIGETEKNLAAIFDQGENKNWILFFDEADALFGKRTNTTDAKDRYAN
                     QEIAYLLQRIEVYSGVTILATNLKTNLDEAFSRRFQSMIYFPLPKPDQRLRLWKTTFK
                     GLRFADNVNLRKIATSYELAGGAITNVLRFCALESFSRDEQLVYHDDIISGIKKELYK
                     DGKTM"
     gene            complement(18583..19500)
                     /locus_tag="PLG24_00190"
     CDS             complement(18583..19500)
                     /locus_tag="PLG24_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23325.1"
                     /translation="MSLNEIIAIASIYQRGFLIIIIGLFLVVLLIFIRIILVRLRTRK
                     KIDASKVKKFQNQDKRLIENAEVKPELNINTEPKDPMTTGIKKISFTRSQRAESFKGV
                     STEEFEQLIGSTGFVMFEMDELWTDYTIKTIYMSLDCCNELNNYLNTENFVQIKNESS
                     VIPEIGGILLGRFNPVDNSLYRISLEEFIPIESVNPDLFSLEFCTESLVKELGDVSEK
                     YPHLTVMGWFHTHPGHGLFLSVPDMTIQMGFFREPYQIAMEIDSLSKNLDTGFFCQKS
                     NGQVNNSSFEKKTWFSWHSIVELLNGQNI"
     gene            complement(19487..21247)
                     /locus_tag="PLG24_00195"
     CDS             complement(19487..21247)
                     /locus_tag="PLG24_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23326.1"
                     /translation="MKLWYFILVVSSLMCQKGVAQGLSDEERAWINIETFSGSNKKKI
                     ALLNDYIKKYPTGDNIERAKIWRHELQNEFQQEVIDDAISSREPQKLNYILKNFPNNK
                     RSEEVRKLIQRLETERWKSLNIRDSTAMRQYIADFPNSEHSDEIFTRLLDLEKGSSTE
                     IASVIVTQEELLAMNVRELQSFLRRYPQSPLVPECLRILDKRENHYYHVILAQVDNFP
                     EFSKLYNKFKELFPESKRLSSLTTTFDTRSREYNINKDKPGFAAWNDLDKSNLEEVLN
                     YGLKYPDSPFKKRITELSVQLEDKLYKKAVMRKSMAALLQYKKMLPNGKHIIEINRLI
                     NELKNNPKYEEYSSLASSKNIESLNKFINANKNNPLVDYALGVVNNVSDILYNLTNDE
                     AGILVQLDNFDNPYLKVSDPDNVILIDSSELKSSGYFVVNFDDVVLNSEITIFDTNGR
                     QRKIQFNNQLKVDFTQAGKILRIRIEGGVPPYVFDYKNSETKFIDQTYSNVMPGKDNF
                     FLVDLDTIENLQGGNYDLRVKSNIGGQMVMLAGIVVDVHDFRLLYFILFTLLLIGLIV
                     WVVRLYIQKSRPKTIFDEFE"
     gene            complement(21255..21986)
                     /locus_tag="PLG24_00200"
     CDS             complement(21255..21986)
                     /locus_tag="PLG24_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-conjugating enzyme E2"
                     /protein_id="HMZ23327.1"
                     /translation="MEKKEYDFDDPLFQRNFLYRRLAKEHLELQKIQSDKIGIEVENV
                     RGPLDIPDTYTIHYYIRSIVGINEDQSPKYSKHHIAEIYLPVKFPMEPPRIYMKSDLW
                     HPNIKWDGKFKGRICGNTKEYGKGYDLVQLVYRIGEILQYKNYHAENTPPFPEDSMVA
                     KWVKEYAEPNKIVDKWSETYTDYSDLTAQSDEFFEDEYQEENNPTQIFTGEIPEKKVS
                     LKISMNTEPSSDEEDSTAKKISFKK"
     gene            complement(21990..22283)
                     /locus_tag="PLG24_00205"
     CDS             complement(21990..22283)
                     /locus_tag="PLG24_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23328.1"
                     /translation="MASKANEISIMLRILPKGEEVGADLHVTTTGKEIISELISNGII
                     PKNDPEGNPYVYELISKNNNAKISEDKTLLDSGIQDGAIIYVTPKLVAGYLSK"
     gene            complement(22291..23490)
                     /locus_tag="PLG24_00210"
     CDS             complement(22291..23490)
                     /locus_tag="PLG24_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HMZ23329.1"
                     /translation="MAKPKTFTIQHEKPWDSTFQLMSWWDSEKVRKACVMVVGAGSLG
                     NEVLKNLALMNVGKIIIIDFDRIESSNLNRSVLYRAEDAEAQSFKSEIAARKIMEINP
                     NVRAIAINADITCQVGLGLFRRCDVIIGCLDNRLARLFINRYASRFNVAWIDGAIENL
                     AGSMAVYRLGKNCYECGLSATEWQNIRYRLGCPDVASRNIKYGKIPTTPISASIIAAM
                     QVQEAIKVIHGNEGFIEYAKKFYYEGMNNIAMWVESNDRYDECESHILWQDVIEDEAL
                     SHEMTIGEFFDYLEGRFENGLKINLDTELVLEITSQESEKSYDVAVLKTELTDDFLAK
                     YRSELVENFFITRSLTCISRESGLNDYTLKSIGIPALAILSIETKDDVIYIELTKDIL
                     KYTILNN"
     gene            complement(23495..23794)
                     /locus_tag="PLG24_00215"
     CDS             complement(23495..23794)
                     /locus_tag="PLG24_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HMZ23330.1"
                     /translation="MLPVARISDFTICPLFTPVPFVGLVPHIGGPIIGPGMPASLIGG
                     LPAACVGDFCYCNLSVNMIASGSMKVMIKGRQLARIGDMTAHGGFILTGTVKILA"
     gene            23981..>25577
                     /locus_tag="PLG24_00220"
     CDS             23981..>25577
                     /locus_tag="PLG24_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23331.1"
                     /translation="MTFLNKKNKEAEMLLFKIREAGFSVILTESKDDYTEFVEHFYVK
                     LLQSKALHINDNNKWKVIVIGYENDIFSETVEKMFERNNFTDRKINLAHESSLISELK
                     QDKTTLLKIYQTFHFAYKFNYLFCLDLYSYEPQVMLDQISKINHLFLDLVESKKVPIY
                     FLLGLDQEQMEIMEDHLPVLFKRNDIEFVFKLPKLKPEEIRDILDEYPIYNQSKKEIQ
                     NEIVDNIYQSDNTLLQLKNAISKFKAGIVPKKDLVIGITSSDERQAQPKKVKATRTVE
                     STQLKNIYEAELDSLYNKLGTRFQRETLCKILLKMMVVEDDGKAYANDCLYQSITENL
                     HILEKDISNALRPFVEKKIISFDPDRDDSIRILKPNAFATWPMFRQMMDKSRSYDLIF
                     EIINQMYDPSDIDLINSLLNQEQLMLINSDDTEFFVNLKASQFKELDDKVSIIRSLAI
                     EVPDGKEVASEPPKANTSPRIKINTDRESTPVPNKNLLITIKKSISESEDISANPEEQ
                     PILETEDAAVDQYIEIISDEGFSE"
BASE COUNT         7495 a         5590 c         4964 g         7528 t
ORIGIN      
        1 gttctttgac cgttttttaa cataatttgt tgaataatgc gcaacttggg ttaaatacca
       61 tagatcgttt gcttaaatgc acctcgttgt ttcacttgtt tctgataaac tctgtctata
      121 tttgtggttc aaacacaaaa gcagccaatg gatcccaatc ctgaagttct ggaaaatgac
      181 gacctgaatg ccgacatacg caagcaggat cacatagacc tggcattcaa gtcggcgacg
      241 gcggctgcaa tgaacgactc cagattcctc tacgagccat tactgggcgt acacagcgaa
      301 tggcaggaag agatggtcat cttcggcaag acattccgac tgccattctg ggtatcgtcc
      361 atgacaggcg gaaccgagaa ggcagcccgg atcaaccggg tattggcaac agtagcaggc
      421 aagtatggtt tcggaatggg gcttggctcc tgccgttctt tgttatacaa tgatgaatat
      481 ctaagtgatt ttgctgtcag gaaatatttg ggagacgatg ccgttctttt tgccaatctt
      541 ggcatcgcac aggtgcaaca actcattgac gaagggaaga catccatgat taagaccttg
      601 ctggataaac tggaggcgga cggtctgatt atccatgtca atccactgca ggaatggctc
      661 caacctgaag gagacagata cacaaagcga cccctggaca caataaaggc gctccttgag
      721 aattctgatt acaaaatcat cgtaaaggaa gtcggacagg gcatggggcc gcagagcctt
      781 gaagccttgc ttgctttgcc cattgaaggt attgaatttg ccgccttcgg gggaaccaat
      841 tttgccaagc tcgaattgat gaggggcagt gaagaaaaaa ggcaggaaat tatgccattg
      901 gcaaaaatcg gccatacagc agaggaaatg gtacagctgg tcaaccacct cggcaaacgc
      961 ctgggcgata atatgcgatg caggcaattc atcatctccg gcggaatcca aaactttctg
     1021 gatattcatt acctaagaag cagcctcaac tacccatcgt tggcaggcca ggccagcaaa
     1081 atgctgcgct atgctcagat cggtgaggga gcattggatg agtatatcga cacacagctt
     1141 aaaggtcttg cctttgctca aaattttctg agaatacggg aagatgccaa tacataccat
     1201 ttgaacaaga aattatagca tttgtagaac gtgaatagcg aaggcacagg aaagacagtc
     1261 agcggctttt caaaattgtc caaaatcgga aagctaaaat ggatcgtgaa taatttcttc
     1321 aaggatcccg aagctgtcac gaaggaaatg aaaagttact ggtttcagga cgaacaggat
     1381 cagaagatca tcgacagcct aagcgaaaat accatttcca acttcgtgct gccatttagt
     1441 gtagcgccta attttatcat caatggccgg gagtatgcca tccccatggt gatcgaggaa
     1501 agcagtgttg tggcagcagc ctcacacgca gcgaaatact ggtcgcagcg aggtggcata
     1561 catgcccgtg ttgtttcgac catcaaggaa ggcagtgtgt actttttctg ggatggcgat
     1621 atctcgttgc tgatgacagg attgccggaa ctggacaagt tgatccggga agattgccgg
     1681 gagttgacgg ccaaaatgga atcaaggggt ggcggaattg tcgatatcgg atttgtcgat
     1741 atgggtcata aggagcctgg ttataaaaaa ctgaatatac ggttcgaaac ctgtgatagc
     1801 atgggagcca acttcatcaa caccatcctg gaggaggcat cttccgtgat gaaaggctgg
     1861 tttgaagcaa aaggttatga ctcacccgac gaaaggcggc tggaagtcat tatgtcgatc
     1921 ctgtccaact atactccgca atgcctcgtt gaagcctccg tggaatgtcc tgttgaggaa
     1981 ttgaccaata ttcccgaaat gacgccacag gcctttgcca ggagatttta taaggcagtg
     2041 cggattgcca cggtcgatcc atcacgtgcc gtaactcaca ataagggcat cttcaatggt
     2101 atcgatgcag tcgtcctcgc taccggcaat gactttaggg cagttgaggc gtgtggtcat
     2161 gcctttgcag caaggtcagg tcattatcaa agcctgagta cctgtacaat caatgacggg
     2221 attttcagct tcactctttt gattccgata gctctgggca ctgtgggagg cctgacaagg
     2281 ctacacccta tagccagacg ctcattggaa ttactcggca atcccggggc tgaggagttg
     2341 atgcaaatcg tggcagccac cggattggca caaaattttg ctgctgtccg gtcactggta
     2401 acaaccggca ttcagaaagg ccatatgaaa atgcacttgc agaatattct ctggcagatg
     2461 aaagcaacgc ctcaggagca ggaattactg atcgaatatt tcgaggacaa aattatttcc
     2521 tactcctctg tcagggcaca actcgaaaaa ataaggagta cgttgtagat gaatatttca
     2581 aaagacgaaa tacccggtat ccattacaat gcaaggggta agctgatgat taccggagaa
     2641 tacctggccc ttaagggagc ccgatgcctg gcatggccca cccatgcgct gcatcatctg
     2701 gaagtacaca cccttgcagg gcagccggga actttgatat ggaatgcttc agacaaggat
     2761 ggtaagccgt ggtttgatgc tttgttttcc acctcagaaa gagtggaaac aatttcttca
     2821 tccgactttt ctgccgcgga ctatcttggt agaatgctga acgcagcgat cgccttgaaa
     2881 gggaaaaaca tcctcgaaca tgcctcattt catgtcgaga caaaaattga atggcccatg
     2941 gaatggggca tgggcagcag ttccagcctc ctggtcaata tagcttcctg gtttggcgtc
     3001 aaccccttta ccctacagca aaaaaccatt ggtggttcag gatatgacat agcttgtggc
     3061 ttgagtgatg aagtcattgt attccaaaat gccggcaaat tattagccaa taaggtccac
     3121 agacctgaga tcctggtaca atatgccgga tttgcctatt tgggaaaaaa ggcagatact
     3181 gcttctgcta ttacagattt ttcttcgcga acagagggca tggatctcac tgccaaaatc
     3241 catctgattg atcgtctgac ctaccacatt ttagacgcaa agacagtaca tcatgccatc
     3301 aaggcaatcg agatacacga agaagtaatc ggctccctgc tggacgtgga acccatcaaa
     3361 ctgagattgt acaaggattt tgacggtgcg gtgaagtctt taggtgcctg gggcggtgat
     3421 tttataatgt ttgtcgggag taaacctttg gaagaattca gggtttctac tgaagaaaaa
     3481 tatggtatta aactatattc agccgctgaa ttgctggttt gaatttaaaa tattacaagt
     3541 atgccgaaat ggatagaagc tacagtcact aagatagtta ccgaaaatga ccgtgtcagg
     3601 cggttgtaca ttgatccccg gaccgaagaa acatttactg ccatcccagg ccagttcatc
     3661 acattcgacc tcccggtagg cgaaaaacgg cttcaaaggt ggaaaagcta ttcaatcgcc
     3721 aaccagatca ctaccggcga aatagagctc tgcatcgcca agcaggaggg tggcctgggc
     3781 tccacctact tatgcgaaga agtgaaggtc ggggatgtat tgaaattcaa aggtccggat
     3841 ggtatgtttt atctgcctga tccactacca ccgactgtag tcatgatttg tacgggcacc
     3901 ggtattgccc cgtttcgcgc catgctccag gctgcactca atcatgataa atacaagaac
     3961 acacaattcc acttgatttt cggtacccga tatgagactg atatcctgta taaggaggat
     4021 ctggaggcat tcagcaaata tcctaatttc aagctggata tagcattaag cagacatatc
     4081 ggagacggaa attaccacaa aggatatgtc cactccattt atctcaatca ttataattct
     4141 gaaaagaccg atgcattgtt tatgctgtgc ggctggcagg aaatggtcga tgaggctaca
     4201 gacatcctcc agaatgccat gttgatggaa aagaccagaa tcatggtgga gttatatggg
     4261 tgatgatcat tgatccatag gggacaggat ctatttgctt tttttcagaa tagacgttgt
     4321 ggaatacccc tcctgaaaat cgatgataac gacctttcca cctgcttcct ctacgatatc
     4381 agcacccacc acattgtccg gcatgtaatc gcctcctttg gttatcacat cagggatcaa
     4441 ggcactgatc agatcatacg gagtatcctc cgaaaaactt gtcactgcat ccacacaaca
     4501 caaggcagcc acgacttctg ccctatcggc cagtgtattg atcggcctgc tttccccttt
     4561 cagccttctc actgaatcat catcattgag tgccacgatt aaccgatctc ccaaagttct
     4621 ggattgggcc aaataacgga catgacccgg atgcagtatg tcgaagcagc cattggtaaa
     4681 tataacctcc aggccctgtt gttgccaggt cctggcctgc aggatcactt cttcccgatt
     4741 gagaacctta tttttcatga ccatcagaaa tcaatatgtg atgtgggata taatgcttgt
     4801 tgttgattgg cagaaatatc agggaaagta caccaaacag gatagtagtg agtatctgaa
     4861 tattgaaaaa tacaatattc gcatagctga aggcatcatc cccgcggata ccataaatgg
     4921 caagcgctgc caccaccagg gcatgataag tccccatacc tccgggcgat gggatcagga
     4981 tgccgaaact accgaatacg aagaccagca acgccgcctg aatccccagg ctcgcagtag
     5041 gtggaaaaga gaaaaaggcg aaataggtca taagaaaata gaagacccat atcagaatgc
     5101 tgtaaaaagc aaacagaaat ggtctttcga tctttctgat cgtcttcatt cctgcaatca
     5161 cccctttagt aaaattctta atcttaagat aaataagaga ttgtctgatt cgatttctga
     5221 aaagtataac cagtagaaaa aacaggacta ttcccgtgac gaaggtctga aatatccaac
     5281 cacgactgaa tgcagcgaag tcgaaatccc tcatattgga tgccagatag ctttgaattt
     5341 cttcatactg gaagaagaat gcaatcaata tggcaatact catagtcacc agatcaagga
     5401 aacgatcgag cgccaccgta ccgaccacat tatccagttt gattttttca tatctggcaa
     5461 gacttccggc tctgatcacc tctcctgctc tggaaagacc tagattggca aagtaactga
     5521 gcataatcgt ccagaaggca tttcctaatt tgattttata gcccaggggc ttacatatca
     5581 tttgccatcg taaagcccgt accacattac taagctggaa tgcgacaatg actgccacca
     5641 accacagaat gttgacggat ctaaagtcag caatcaattt ccggatcaga ctgcattgtt
     5701 cttccggaat gccatcaata gcgcattgct cctgaaatgc cctgctttga gatatataca
     5761 cccaaaacaa aataccggca ccgatcgcca agaacaacaa aaacttcaga aaatcaacaa
     5821 tgaacttttt ccgctgcaac gtcacaccag tttgttgttg ctatcaggga aaacagcggc
     5881 aggcttgaat gaccgggcct cttcaggtgt cataaatcca taggcgataa taatcacagt
     5941 atcacctacc tccactttgc gtgcagcagc gccattcaga caaatgtctc ccgagcctct
     6001 ctccccccgg attacataag tttcaagcct ctcaccgttg ttattattaa ctacctggac
     6061 gcgctcaccc tcatagagat tagcagcttc catgagatct tcatcaatgg tgatgctacc
     6121 aacataattc agattggcct ctgtgacagt cgcacggtga atttttgatt tgaatacctg
     6181 gattagcata gtgggcacaa atttacggtt tttaattgga ataaaaaaaa cagtgacaac
     6241 agatacctat tgtcaccgaa tatattatta aacagtgtat tgcttgtcaa agtttctccg
     6301 gattatattt tcctttccaa cagcttgaag aactgatcca attgtggcat gataatgatt
     6361 cttgtccttc tattgacaga ccttccttct gcagtgtcgt tggtagtaag tggggaaaat
     6421 tcactcttgc cacctgctgt tatcctcttc ggatcgatgc cgaaccgggt ctgaagggca
     6481 cgtgctatgg atgtggctcg caaggcactc aagtcccagt tatccttgat tcccggcttg
     6541 ctgattggaa cattgtcggt atgtccctct atcaatattt ccagatcggg ctgattgttg
     6601 agcacctgag ccaccttggc caatacagct cctgcatttt ctgaaatatc atagcttcca
     6661 ctcttaaaca acattttgtc cgaaagtgaa atcataacaa tgcctttgtc caccttaacc
     6721 tcgacatcct gatcattgag attacccagg acacctttaa ggttcatgac aagtgacaga
     6781 ttgagcgaat ccttacgggc agcagcttgt tggatagtct ggatataatt atccttcgca
     6841 ccaatgtttt ccaatgattt cttgatggac tcagcctgac tgttggtaat gacggacaaa
     6901 tccttcaatt gacccagcac agctgtattg ttctctctca ggaagacgat ctgtttattg
     6961 agttcttcga tttctttgtc agaaacacgc tttctcttgt ctgcttcatt gaccgctgtc
     7021 tcacaatcaa tcaatttcct ctccagtgca acatattgat cactcaatgt cttatattgt
     7081 tcttgtgcag cttttagctt tttacctgtc ttacaagaag atccggtaat gatcagactt
     7141 gctaaaagta aaaaatggaa tagtctcatt aatggaattt atttaagaat acgtaaacta
     7201 attgaagtaa aaggaaatca actacttttt gttcaatttg accgcaaaat taacaataaa
     7261 tgactgaatg ataggcagtg aaacagaaaa aattggatat atctcagttt ttataccctg
     7321 aatagctcaa aaaaatgaat gggatgcaaa tattgttaac gacaattgtg gaccaaatcg
     7381 aagtaggtag gaaaataata atctaaagct gtgtatttat ccgcctttta aatgttttca
     7441 cagaatatgg actaacattt gacatcaatt tacaatttga ctattctaca ttgagcacat
     7501 actcccgctt ttgggttatt aatcatctac ctaattcagg acgacccggc caaatcagat
     7561 ttcacggcac tcgctttccg tttactaaag atcatttcaa attatgtcca atgatgatca
     7621 aaaattatta ctcaatcaaa aactacaatt ttaccctaat tgaaaacatc ctgtatcacc
     7681 aacaggtaga aagtcctgaa agcgtaaatt acatataatg gctatatcaa acaattctcc
     7741 atatagttat aacacacact atccctcagg atacaatcaa tcttcagaga aaaaataatt
     7801 gatgatggag gtgatttcct gatccttctt caattcagga ttgagtcggt aaagaatgac
     7861 atgattgtca aaatcctcac tcaacgcttc tttcaatact tcacaggctt cctctttatc
     7921 atcctgagca attaatacca ggaccttgta ataaagcaac tcagaactgt aagtatattc
     7981 atcagcctct tccaaaacat cgaacgcacg atcaaattct tctatttcat agagaaattt
     8041 gacataggaa agccagattt cgggaatatc gccgccaaga tcagtggctt tcttgaaata
     8101 ataatcggcc ttatctgcgt ccccttgctt atagaaggct tctgcggttg ccgcaaaata
     8161 ttcctccctg ctgtcttcta tcttcaatgc cttgtaatag tgctggagcg ctatgtcata
     8221 cttatgttcg gcagcatagc aaagacccag gtagtaatgc acttcgtcat tgtatgggtc
     8281 cagtttgttg gatttgcggt agttcagtcg ggcaagccga tattctttca gcttataata
     8341 acattcacca atgcaagcga aaagttcgct atccgggcca atattatcca atgcctcaag
     8401 atatactttc agcgcaaacc gatagcggca catttggaag gctaaatctg cacaatcttt
     8461 ataccccagc tcaaaggaag ggtggatcag aaagctgtat tcgtaagctg ccagagaatt
     8521 ttcatattcg ccgatacagg aatacgcctg tccaagattg taccaagcca tataagcata
     8581 cggatcattg tccagcaact gattgagata ggttattccc tggtggtaac tgcgctggct
     8641 ctccatataa aaccacaatt tttccatggc caattcattg gagggctcaa gatcaagtac
     8701 attcctaatc tgattaaaca tctgcgtata atctttcaac tcctcatgga tccgagctct
     8761 gagcaacatg gtattgatcc ggtccttttg agaccctacc atcatgatat cgtttaaaag
     8821 attaaatgcc tgatcatatt ctttattaat aataagacag ttggctttga gcaacttggc
     8881 ctcattctct gaaggtgcca gcaacaatgc ctgatccaca gcttcgaatg cgtctgatgt
     8941 attgttgagc gctagtaaga tttgagcctt cttcaggtag agatcgggaa ggaaattgaa
     9001 tctttctatt gccaaatccg ttatctccaa agctttatcg agcatcaact ccttctcgaa
     9061 atataatata agttctataa agtccttttc atgtagtaag ctcaggccgc cattccttga
     9121 catcgcttca tactcactga cgatattggg aactgaatta ttgttagtat taaggtttcc
     9181 gtccatataa tctgttcact taaatgggat ccattcaatt cggcggtaaa tatatagtta
     9241 atctcattga accgagcact tttttttcac atattttttt ctgtaaacct tgattttaca
     9301 aggcattaca aagattttgc atgtatggaa tcaactttaa aacagtcatt taagtatcac
     9361 tattttttaa ataccttttc acgagctatt caatcaatta aaagacaaat gataaaatat
     9421 tacacggaaa aataatgtta aagaatggta ttattatttg attatcagat actaacaata
     9481 aagctgttat atgttaatgg gataaattgt ggaaaacttt ttatgaatgt gaaaaaactc
     9541 aatccagttt aagctcatta tccgaaactt ttcatgttcc attggcaatt atgcttctat
     9601 gatttcaaat aaaaggggct gcattacctt acaaaaatgc gggctcttgc aattattatc
     9661 cttactgcca aaaggcctca tctaaaggct cttcattggt ctgcaaaatc caattagtca
     9721 ctttacagac atgatgaatg tattttattc aaagagatgc cactatccaa tacttgttta
     9781 ctttcaaatc atatattgta cttcgaggtt ttttatacct gctcttataa gttaatacaa
     9841 tgaatcctat gttccggctc ttataaatac atgaaatttc tctttattta taaaaataca
     9901 ccgggttatc atttttatag aaataaccac gccatgcctg tctgaacagg acgaaaatgt
     9961 catcttttgt gatgagtaat caacaaaggt ttgaatcgag aagcaattct ggatactaaa
    10021 ccgcttttgt tgctatgatc cggatctgga taatacaatt tcccgggcgc atagttcggg
    10081 gaattgctca ggggtagatg cggcacagac tatccccaat tcagtaaatt tattcgcatt
    10141 ttcgacattc caatgacctt ttccttcttt ggtaagcgcc aatatggtca aaaatcggtt
    10201 gaatgtactt gcaacacgcg cagcttcccg cataaaggtg acttcatctg atgtttcatc
    10261 cagatcggag ataaggacca tcaaagtacg gtccggatta gagcaggtct tgtgcgcata
    10321 ggccaatgct ttccctatat tggttccccc tcccagttgt gaagcaaaaa gcacttccac
    10381 aggatcatcc tccattccgg tgaggtcaac tacttcggta tcaaatagca ccagatgtgt
    10441 cttcaatgcg ggaatcatag aaaatatgct tgcgtagatt gcagcataga ccagggattc
    10501 atgcatggac cccgactgat ctaccagaat gaatagtgtg tcgatggttt tctgccggtt
    10561 ttgacgacca ataagccttt caggaataat ggttcgctta gcgggttgat aagtttccag
    10621 attcttgtat atggtcttga cccagttgat atgattgtat ttcggccggc gattgggtat
    10681 gggcgacctg agagccttcc caatggcctg tgttgtttgg aatgcgaaaa ggccggttag
    10741 cttcctggcc aattctttta caataagtcg ggctttgtgc tttgcgacat cgggcaacag
    10801 gcctttcatc ttgaggatgg aaactgccag atgaatgtcc gggacaatat gttccaaaaa
    10861 ggcgtcgtcc ttgagcagca attcaaggcc atatcgcttt attgcatcct tctgcaatac
    10921 tgtactcacg ctgtttggaa aataaaagtc tacctcccgc aaccaatcat tgacgctaaa
    10981 gttcctgttg gacgagcccg caccaccatc cccataaacc catatcagtg ccttgtcgat
    11041 ggtttcctcc tcatcggacc attccacacc ctcaaaatct tcgtccttgg caggattggc
    11101 actgaggatt agtgaccatt tcttcagcag gtccgccttt cggtcttccg aaaaatctgc
    11161 aattaactca ctcaaccctt attccgtgac atgccattaa acaaccagat caaaataaag
    11221 gctcctacaa cagatgtcag aaaattaccg atccctgtta ttcccagttt attgaagagc
    11281 cagctcccga aagcgccccc aagtactccg agaattacat tgaagattat accactgcct
    11341 ttgcctttaa acaataagct ccccagataa cctgctgctc cactgacgat gatcgtccaa
    11401 atccaattat ctcccatgat aatatatgag ttaatgtttc aaaaaaatgt atcttggctt
    11461 tatattttga gttaaagctg agattgatgg cttttcccca acaaatattc cggggaaaat
    11521 gccgctactc aacaaatata cgattaattt atatgaataa aaactttcgt ctcgccatat
    11581 tatttttgtc gattattatg atgggtgggt atggttgcag acattcagca cccgattgga
    11641 gcagtcctgt caggatttac gacactctgt gttcgtgcaa gaatagttca ggacatactg
    11701 ttgatgcagg actgaaaaaa atgctggacg aaaaaaatat tcaggcagag aatgccgatc
    11761 agatcaatac atggatatcg gccaatgcaa cagcgtttgt tgcatttgtt gactcggatt
    11821 tcaaaaatga ccctgtgttc atgagcgagt tgaatgcagc gagagatacg ctggaggctc
    11881 atggtgccag gcttgagacg gaaggtgctt tcactgaatt gttctccatc aaggtcaaat
    11941 accccggatg tgttttggcc atgccctacc tgattacaaa ataaaaagag gtactcagga
    12001 aatgtatagg tgataccaaa ctcccttgcg ttcaattaaa ataaattgtc tacatattca
    12061 taacaaggat aaagccatgt caaacgaagt aaatatctac gaagaaatcg tcaaactcaa
    12121 tatgcggata tcctgcctcg aatctttgtt gcagcacatc attgaaatta acccctatct
    12181 cgaaattccg cccaaggaag agatcgacag gatatacagt aaagcaatcc agcaagtgat
    12241 tgatgaagtt gaagcgggct gatatcgact cttaatgccg gaatttggta attggtaata
    12301 aactaagggc agaaacgcac tattctttcc cggattgacc aaagggaaaa ttagactcct
    12361 ttgattccgt ttcccctatt ccaacaatga tgattattct ccaatagcaa tgtacgccta
    12421 ttgtttacta aaacttcgaa tatgtcataa aacaatcggt ttccactgct taacctataa
    12481 caggctctac attgggtcaa agcttcccct accggacaat taccatcctc ttaactcatt
    12541 ttgcctgctt ttaatacctg cccgtctacc aattgaactg caaaggcagc acaacctccg
    12601 aacgaaccgg ctttccacct atttccgggg ctatccatgc aggcatggta ttgaacactt
    12661 tgttgagggc taattccatt tcaggcagga caggcactaa ataagtaata agttcaatct
    12721 ttccattaga atcaaaattc aaccgaaccc ggacatcttt ggttttagtt ttcacatagc
    12781 catctacttt cagattgctt gctatgaatt taccgagggc ttcctggccg cccggatatt
    12841 ggggtatgta tttatctggc ccggttgctt ttttcaccac gcgcatctgc ccttcaggag
    12901 ttatgtatat ttcccgagat atcgctggat tgtcatcctg ttgattaaaa ttatttactt
    12961 tggccgtaat tgcattcagg tcaatccttt ccgcccatat aaaactcaaa agcagaaaag
    13021 ggaggacgga gaggtaaatc aaccgatgcc ggcccgacgt cggtgtttga accatcttcc
    13081 tgaaccggct tttcaactcc gacttagcaa agtgatgcgc taccgagacc gatgcaccca
    13141 tttgctgaga cagcagcagc tcaccataaa ttctggtatc cattgtcttg aggacttctg
    13201 catctgccag atattcatgt attaatgtca aatatttctt aaacaaaaac accaccggaa
    13261 aaaaccatgt aaacacaaac agaatttctg tgagaattat atccatcgag tgccattgtt
    13321 taatgtgtgt ggattcatgc cggacgatcg gcacaagtga ttcggcatca ggtctgttct
    13381 ctttactgag aaaaataaga tggaaaaatg aaaacggggt atgtcgctga ggtgactcca
    13441 cgattcgcat gccattgagg taggtggagg aatttcctcg agccatcata taaagtgtca
    13501 gtaccttata agccagaata gccaacagga tgcaacttac aatgtaataa acagccaaaa
    13561 tggtcgtcca tactgtctca gggacggcgc catgacctat ggttattccg cttatgatgg
    13621 aattcaggtt atcagaaatg aagttatcac ccttaatcat ctcaactaat ccaaaattca
    13681 atttgccttg cggaagaata ttaataacgc cattcaacaa ccctatgacc aacgagccta
    13741 aaagatatcc gcgttgcaag aagaaaaaag tagattgtct cagccacagc acatagggaa
    13801 tcaacaaaag actccatgtg atgatggcta cgatggcgaa tgtgaagatt accatgaccg
    13861 ttacttttta ttaaggatat ccatcaaatc attgaattcc tgcatattca gatcttgctc
    13921 ttttgctatg aatgacacca atcccgaaaa agagccatca aaataatcat tgacaaactt
    13981 gttgagtgaa cggcttgagt actcattctt ctccaccaat ggaaaatatt catacgcacg
    14041 cccaatagct tcgtgtccca gaaaaccttt ccgttccaaa atcctgacaa tggtagacac
    14101 cgaagaatga ggcggtttgg gatcgtccat acctgcaatg atatctgata cagaactctg
    14161 ccccttctcc caaaagtgat gcataatctc ttcctcagcc ttggtcagca ttctttcctc
    14221 atcgctcata atattagtcc ttttttaact tatgacattc tatccaacaa ccatcttcat
    14281 gtttcaaccc atgaaaacaa tcatcttaac ttgacaacgt aaatttacgg tcaataattt
    14341 catcttacaa cttaattttt agttaaagag gataaaatta gaatccaatc ccgatatgga
    14401 ttcccggact gatatggata tcatcggcat cataggaaaa ttcaaacacc ggcccgatat
    14461 ggattccctt gatgtcgaat tcatatactg cctctatgtg cagtccgaat tgtggcccgg
    14521 catgcttggc atcttcatac gcaagtcccg gtgataatat aaggctgaat gcaccggtag
    14581 gccggtaagc agccactaca cccagaaagt ggtgcgcatg atccccgaaa attctctcat
    14641 agccggcacc aactccaaat tttgattctc ctaaactcct tagataatgg aggtgtaagc
    14701 cgaaattgaa ctcacgtgta tgtgtaaaat aggccggtgc cactactacc ccaatttcat
    14761 tcttatgatg ttcatgacct tcttggtgct catgatcatg ctgatggcca tagtgcgatt
    14821 catcagaatt ttgtgcaata ccggacacag taatcaataa cgtacaaaat acagttaata
    14881 ctttcagtct tatattcact tttgatgccc tgctaacatt aatatttggg ataatgcgat
    14941 gtcctgaatt tttattgttc atttaagtcg agtctgattc aaatattgct gcaatataag
    15001 agagctgttc cttattataa aataattaat agaaatttaa agtgtgttta aatatcaata
    15061 cttgccttaa tgtaggatca caatgatctt ccttccttta taatcctgat tcaatcacgg
    15121 ccttctctgt tagattatga ctgattggct caaacgtcct atcatcatcc aaagattgtc
    15181 cggtgagaaa cctaagatca aacttttctt gagaacttaa aatgaatctg tcttgttgct
    15241 ttaatctttg agccagatac tcctgttcag gctgaccggg agtcggcacg agaattcctg
    15301 aagcccccaa tgccatcaga tccatgaccg tagtataacc ggtccgagaa atgaccctcc
    15361 tgctttctgc aatggcatag gatagctctt cggtagtcat gttattaacc caggaaatac
    15421 cggaacctga gtaatccggc ggaggcgtct ggtcttccac ccccctgaca agcatatacc
    15481 ggtatggtgt taaagatgcc tgacttataa taatctgctc aaaatcactc ctcgccggtt
    15541 caggcccgga aaggatcact ataaagtccc tgataattgg ttttgaaagt ttctgcagtc
    15601 gggataaagg accgatgtaa acaggatgta agctgccaga tgatgacata tatccactta
    15661 ggttttgggg aggtggaaag tcgggtaccc atatccgatc aaaatgacgg atcaggaacc
    15721 tgttggccat cccggcaaca gcactcatga gtcgaagcgg tgtcagtatc ctgagttgat
    15781 gtgttatgaa gatattgaca caatcaccgg aataacagcc aaatctgtta tcagagagga
    15841 tgacatccat gctgttttgc tttacataag cctgaacaaa cctgtgttcc aaaatcacag
    15901 tcttcaatat gcggggcaac tgcgcggcta cggttcttag caggttcttc cccttataat
    15961 ggatgtcgta tccaggtaat tggatggcag gcaagccggg gaattccttt cttaatagtt
    16021 ccaaggcaat gccatcactt gccaaatgaa cttcatgccc acgctgaatc aatagctgga
    16081 taatgggaac acaacgggtt gcatgcccca aaccccaatt aagcggacaa acaagtatct
    16141 tcttctttat cattagaata cgggagtcat aaaaattact cctattgaat aactttcagc
    16201 acaaaggtaa gcattgaatg ggtataacac ggtgactatg caccattgat atccttcatt
    16261 actttgaagt tatattgata tcctggagat ataaagcaga ttcaccactg tcatcaatga
    16321 caaaaaggat tattttcatg gcatcctgcg atttattctg gaatgatgta atctttctgt
    16381 tgcaataata cacttttgtg atggaccctg tattactaat ggcaaaggtg tttctgttta
    16441 cctctgagtt gacaggtata tatttctttt tcctgacaag aggtttagta ccactcatcc
    16501 ttgtcttggt cagaatattc agttcatcct ttgaggcggg tttgttgatc tgaaccatca
    16561 cataattgta aaggctatcc atctcatggg catccaatcc cgaatcggaa aagaggcgat
    16621 tataatcatt ttcaccgacc agagcaatgc tgaaattgat atcggactta ttactttcac
    16681 caacctgggg aatgaggttc atttcaatag cccaatcccc attaattgcc tgggacatga
    16741 tcggacaata agcagaatta ggtaacagtt taatccattt gcctttgaat ccatccaact
    16801 gtaccaattc aattacctga ttgaaggatt ctgttgtctg gttatcatcc ttcatgttat
    16861 tggcattgaa agacagcact gacttggaca ttggcttgaa atcgtacttc tcggttactc
    16921 cggtttgtcc aggctccttc gtcttgctat ctccggtttt cttacctgaa tctttctggg
    16981 gtttgctttt catgctattg cgaatatcct tttcggcatc attgatggtg cggtctatcc
    17041 cacgattaat gcctgactcc acctttcttt gaattttttt attggtgcgc tcaccgattt
    17101 tcatgatatc ctgaccggag gccgccactg caaaagataa gatggttatt accaagatca
    17161 tgcttctcat aatccgaata ttttattagt tattaatcca gtttctctac taaaacatct
    17221 acattgtctt tccgtctttg tatagttctt tcttgatacc acttatgata tcatcgtgat
    17281 aaaccaattg ttcatctcta gaaaagcttt ccagggcaca aaatctcaga acattggtaa
    17341 tcgcaccgcc tgctaattca taactggtcg cgatcttcct aagattgaca ttgtcggcaa
    17401 atctgagtcc cttgaaggtt gtcttccaaa gcctcagacg ctgatcgggt ttgggtagtg
    17461 gaaaataaat catggattgg aacctgcggc tgaatgcttc gtccagattg gtttttagat
    17521 tggttgccag gatggtcaca ccagagtata cctcgattct ctgcagtaag taggcgatct
    17581 cctgattggc atacctgtcc tttgcatccg tggtatttgt ccttttgcca aaaagagcat
    17641 ccgcctcgtc aaaaaacagg atccaatttt tattctcacc ctgatcaaag atcgctgcaa
    17701 gattcttttc tgtttcacca atatacttcg atactacttt tgacagatcg attcgataca
    17761 cagtcaggcc aacctgcttt cctatcaagc ttgctgtcag agttttcccg gtcccgggtg
    17821 gtccgtagaa aagtgccctg taacccggat tgatatattt gtttaaatcc gtattctcaa
    17881 ggattaaatt ttcaagacga acccatttaa taatttcttc aacctgatgt agtgtgtatt
    17941 cttcaagaat caaatcatcc caggcaagtt ttgtttccag cttatttgca ggaaaatcac
    18001 tggaaaaccg tgggtcgaat cgtttgtctt ctgttagaat gctaagaaac tccttgctta
    18061 tcgagagttt gccactcagc atgggttcgg agtttccatc tttttccaaa ttcaagatcc
    18121 gatgtttgat aaatggatgt tccggcgcga aatatctcat gatcctgaat ctatccgcga
    18181 tatcattaag cgataaaagg aaaactgccg tttctccggt aggcaaaaag ccaacatgat
    18241 ggagtccttt taaaccacca aattcagaaa aaggccttgc ataggttgaa ttttttacaa
    18301 agaacgcatc caaaagctgg gggcacacgt ggggagccat tgccatcagg aggacgactc
    18361 tttcttccat attcaggtta taatacctgg taaattcggc ataagtggaa ttatcctttt
    18421 gcaaatcaca ggcaactaca tcctttactc cttcatcctg accaaaatat ttagcaaatt
    18481 gtgatgtaat gaaatttgcg agccaattga attccttact cagagtcact gcattgggcg
    18541 acctgttatc gttgttcgga tcaaaaacca gttcaaaact cctcatatat tttgtccgtt
    18601 aagtaattca acaatcgaat gccatgagaa ccatgttttc ttttcaaatg aactattgtt
    18661 gacttgtccg ttacttttct ggcagaaaaa tccagtatct agattttttg aaaggctgtc
    18721 tatttccatc gctatttgat aaggctccct gaaaaagccc atctggattg tcatgtcggg
    18781 gacagaaaga aaaagtccat gaccgggatg tgtatgaaac caacccatga cggtcaggtg
    18841 tgggtatttt tctgaaacat ctcccaattc cttgacaagg ctctcagtgc agaactccaa
    18901 actaaaaagg tcaggattga cactttcaat ggggataaat tcttccagac taattctata
    18961 caatgaatta tctaccgggt tgaagcgccc cagcaatatc cctcctattt cagggatgac
    19021 tgatgattca tttttgattt gaacaaaatt ctcagtattc aaataattgt tgagctcatt
    19081 gcaacaatcg aggctcatat aaattgtctt aattgtgtag tcggtccaca gctcatccat
    19141 ttcaaacatc acaaaaccgg tagatccaat taattgctca aactcttctg tgctcacccc
    19201 tttgaaactc tctgcccttt ggctacgggt aaaacttatt tttttgattc cggttgtcat
    19261 tggatcctta ggctctgtgt taatattcaa ttccggcttg acttcagcat tttcaattaa
    19321 acgtttatct tgattttgaa attttttcac ctttgaggca tctatttttt ttctggtacg
    19381 taacctcacg agaataattc tgatgaatat caggagcacg actaaaaaca atccgatgat
    19441 aatgattaaa aaaccacgct gatatatgga tgcaatcgct ataatttcat tcaaactcat
    19501 cgaatattgt ttttggtctg cttttctgta tatacaacct aacaacccac acaatcaggc
    19561 cgataagcaa taaggtaaac aatataaaat aaaggaggcg aaaatcatgg acatccacaa
    19621 cgattccggc taacatcacc atctggccac cgatattact tttcacacgc aaatcgtaat
    19681 tcccaccctg tagattttct attgtatcca gatccaccag aaaaaagtta tcttttccag
    19741 gcataacatt tgaataggtc tggtctatga atttagtttc actgttttta taatcaaaaa
    19801 cgtatggagg aactcctcct tcaatcctga ttctcaagat tttcccggcc tgagtgaaat
    19861 caacctttaa ttggttattg aactgtattt ttctttgtct tccgttggta tcgaaaatag
    19921 taatttctga gtttaaaacg acatcgtcaa aattgactac aaaatatcct gagctcttta
    19981 attcgctact gtcaatcagg atgacattat caggatccga tacttttaaa tacggattgt
    20041 caaaattgtc cagttgaacc aatatgcctg cttcatcatt ggtcaagttg tagagaatat
    20101 cagatacatt attgactaca ccgagtgcat agtctaccaa cgggttgttt ttattggcat
    20161 tgataaattt attcaggctt tcgatgtttt tggaagaagc cagtgatgaa tattcctcat
    20221 atttagggtt atttttaagc tcatttatca acctatttat ttctataata tgttttccat
    20281 tgggtaacat ttttttgtat tgcaataagg ctgccattga ctttcgcatc acagcttttt
    20341 tatacaattt atcctccaat tgaacagaca actcagtaat tcgcttctta aagggggaat
    20401 caggatattt taagccataa ttcaatactt cttccaggtt acttttgtca agatcgttcc
    20461 atgctgcaaa tcccggcttg tctttattga tgttgtattc ccgggatcgg gtgtcaaatg
    20521 tggtagtcaa ggagcttagt ctcttacttt caggaaaaag ttctttgaat ttattataca
    20581 gcttgctaaa ctcaggaaaa ttgtccacct gggcgaggat cacatgataa taatgatttt
    20641 cccttttatc caaaatccta aggcattcag ggacaagggg tgattgagga tatcgcctca
    20701 ggaaactttg caattctcgt acattcattg ccagaagctc ttcctgagtt actattacgg
    20761 aagcgatctc agtggaggaa cccttctcca aatcaagtag tctggtgaaa atttcgtctg
    20821 agtgctcact gttgggaaag tctgctatgt attgcctcat tgctgtacta tccctaatat
    20881 ttaaggattt ccatcgttcc gtttcgagcc tttgaataag ttttcttact tcttcagatc
    20941 ttttattgtt tggaaagttt ttgagtatat aattcaactt ttgaggttcc cggctactga
    21001 tcgcatcatc gataacctcc tgttggaatt cattctggag ttcatgtcgc catatttttg
    21061 ccctttcaat attatctccg gttggatatt tcttgatata atcattcaac agggcaattt
    21121 tctttttatt gcttccggaa aaggtctcaa tgttaatcca tgctctttcc tcatcggata
    21181 gaccctgggc tactcccttt tggcacataa gggagctcac tactaatatg aaataccata
    21241 atttcacatc ttaattattt tttaaaagag atcttcttcg ctgtgctgtc ctcctcatcg
    21301 cttgagggtt ctgtattcat tgatatttta agagaaactt tcttttccgg tatctctccg
    21361 gtgaagattt gagtaggatt attttcttcc tgatactcat cctcaaagaa ttcatccgat
    21421 tgagcagtaa gatccgaata gtctgtgtag gtctctgacc acttatcaac aattttattg
    21481 ggctcagcat attctttcac ccatttggct accatgctat cttccgggaa gggcggtgtg
    21541 ttttccgcat gatagttttt atattgcaga atctcgccga ttcgatatac aagttgtaca
    21601 agatcataac cttttccgta ttccttcgta ttaccacaaa ttctgccttt aaacttacca
    21661 tcccatttaa tattgggatg ccacaaatca gacttcatat agatcctggg aggctccata
    21721 gggaacttga ccggcagata aatctcagct atgtgatgtt tactatattt tggcgactga
    21781 tcttcattga ttcctacaat acttctgata taataatgta tcgtatatgt gtcaggaatg
    21841 tccaaaggtc cacgcacatt ttccacttca atccctattt tgtcagactg gattttctgg
    21901 agttcaagat gttcctttgc gaggcgccta tataaaaagt tcctctgaaa aagaggatca
    21961 tcaaagtcat attctttttt ttccattatt tatttgctta aatatcctgc aactaatttg
    22021 ggagtgacat atatgatagc cccatcctgt atgcccgaat ccaacaatgt tttatcttca
    22081 cttattttag cattgttatt cttcgaaatc aactcatata cataaggatt tccttccgga
    22141 tcatttttag gaataatgcc atttgatatt agctcgctga taatttcctt gcctgtggtt
    22201 gtaacatgaa ggtcagcgcc tacttcctcg cctttcggga gaattctcaa catgatgctt
    22261 atttcattag ccttgcttgc cataatttat ttagttgttt aatattgtat attttaaaat
    22321 atcttttgtc aattctatgt atatcacatc gtccttggtt tcaatactca aaatagcgag
    22381 tgccggtatt ccgatggatt tcaatgtgta atcattgagg ccggattctc ttgatataca
    22441 tgtcaagctc cgggtaataa aaaaattctc aacgagttca gacctgtatt tagccagaaa
    22501 atcatctgtc aattccgtct tcaaaacagc tacgtcgtag cttttttcgc tttcctgtga
    22561 cgttatttcc aaaaccagtt cagtatccag attgattttc aaaccatttt cgaaccgtcc
    22621 ttcgagataa tcgaaaaatt cgccgatagt catttcgtgg ctcaaggctt catcctctat
    22681 aacatcttgc catagtatgt ggctctcaca ctcatcataa cgatcattgc tttccaccca
    22741 catagctatg ttattcatgc cttcataata aaatttcttt gcatattcga tgaagccctc
    22801 attgccatgt atcactttaa tcgcctcttg tacctgcatt gctgcaatga tggacgctga
    22861 aatcggagta gtaggaattt taccatactt aatattgcgg ctggccacat ccggacatcc
    22921 gagacggtac cgaatattct gccattctgt cgcactcaga ccacattcgt aacagttttt
    22981 ccccagtcga tatactgcca tggagcccgc caggttttct atagctccat caatccatgc
    23041 aacattgaat ctggaagcat atctattaat aaataatctg gcaagtcgat tgtccagaca
    23101 tccaatgatt acatcacacc ttctgaataa gcccaatccg acttggcaag tgatatcggc
    23161 attgatggct atggctctca cattgggatt aatctccata attttacgtg cagcaatctc
    23221 ggatttgaaa ctttgtgcct ctgcatcctc agcccgatac aatacagatc ggttcaaatt
    23281 ggaagactca attctgtcaa aatcaatgat gattatcttc cctacattca tcaaagcaag
    23341 gttcttcaat acttcattac ccaaagaccc ggctccgaca accataacac aggccttcct
    23401 taccttctca ctatcccacc atgacatgag ttggaaagta gaatcccatg gcttttcatg
    23461 ttgaattgta aatgtcttag gttttgccat atcactacgc taaaatctta acagttccgg
    23521 ttaatataaa cccaccatgt gctgtcatgt ctccaattct tgccaattgc cttcctttta
    23581 tcatcacctt catggacccg ctcgcgatca tattgactga aagattgcaa taacaaaaat
    23641 cacccacaca ggcagcaggc agtccaccaa tcaaacttgc aggcataccg ggaccaataa
    23701 tcggaccgcc aatatgaggt actaatccta cgaatggaac aggggtaaat aacgggcaaa
    23761 tggtaaaatc actaattcgg gctacaggca acatagagca gattcatttt ggtgcggagt
    23821 aaagatagtc aattaatttt atttatcgaa attacacaat cgtttattat aattatttaa
    23881 aaaaaagcta tatattgcct tgttttaaat taagaatccc attggttatt ttaaacttca
    23941 tgcttaattc caaatatttt atttattcaa agttatactg atgacttttt taaataagaa
    24001 aaataaggaa gcagaaatgc ttctctttaa aatcagggaa gcgggcttct cggttatact
    24061 taccgaatca aaggatgatt atacagagtt tgtcgagcat ttttatgtca aattacttca
    24121 atcaaaggct ctgcatatca atgataacaa taagtggaaa gttatagtaa taggatatga
    24181 aaatgatatc ttcagtgaaa ctgtggaaaa aatgttcgaa cgtaacaatt ttactgacag
    24241 aaaaattaat ctcgcccacg aaagcagtct tatttctgag ctaaaacaag ataaaaccac
    24301 cttactgaag atctaccaaa cctttcattt tgcttataag ttcaattatc tgttttgtct
    24361 tgatctgtat tcatacgaac cccaggtgat gcttgatcaa atcagcaaaa taaatcacct
    24421 gttcctagac ctggttgaat ccaagaaagt gcctatatat ttccttttag gcctggatca
    24481 ggagcaaatg gaaatcatgg aagatcattt gccggtttta ttcaaaagga atgatatcga
    24541 attcgttttt aaactaccta agctcaaacc cgaggaaatc cgggacattc tggatgaata
    24601 tcctatctat aaccagagta aaaaagaaat tcaaaacgaa atcgttgaca atatttatca
    24661 atccgacaat actttactcc agcttaaaaa tgcaataagc aaattcaaag caggaatcgt
    24721 tcccaaaaaa gaccttgtta tcggaataac ttcttctgat gaacgacaag cgcaaccaaa
    24781 aaaagtaaaa gctaccagaa ccgttgagtc gacacaacta aaaaatatat atgaagccga
    24841 actggattcc ctctataaca aactgggtac ccggtttcag cgagagacac tctgcaagat
    24901 tcttctcaaa atgatggttg tcgaagatga tggaaaggcc tacgccaacg actgtctgta
    24961 ccagtcaatt actgaaaacc ttcacattct tgaaaaagac atctcgaatg ccctaagacc
    25021 ttttgttgaa aagaagatca tttcgttcga tccggacagg gatgacagta taagaatact
    25081 gaaaccgaat gcttttgcga cctggccaat gtttagacaa atgatggata aatccagatc
    25141 ttatgatttg atttttgaaa taataaatca aatgtatgac ccatctgaca ttgatttaat
    25201 caatagcttg ctcaatcagg aacaattgat gctgattaat tcggatgata ctgagttttt
    25261 cgtaaatctc aaggcttcac aatttaaaga attagacgac aaggttagca tcattcggag
    25321 tcttgccatc gaagtccccg acgggaagga agttgcttct gaacctccaa aggcaaatac
    25381 ttcgcccaga attaaaataa atactgaccg tgaaagcact cccgtaccga ataaaaatct
    25441 gttaatcacc ataaagaaat caatatctga gtcggaagac atttctgcaa atccggagga
    25501 acagcctata ttggaaactg aagatgcagc cgttgaccaa tatatcgaga tcataagtga
    25561 cgagggattt tcagaat
//
LOCUS       DAOQQN010000003        24424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_614_length_24424_cov_5.562969, whole genome shotgun sequence.
ACCESSION   DAOQQN010000003 DAOQQN010000000
VERSION     DAOQQN010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 24424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24424
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_614_length_24424_cov_5.562969"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PLG24_00225"
     CDS             complement(<1..717)
                     /locus_tag="PLG24_00225"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HMZ23332.1"
                     /translation="MKFKTIIEPFRIKSVEPIRMSTEAERIENIHKAHFNPFLLHSSQ
                     VVIDLLTDSGTSAMSSDQWAGIMRGDESYAGALSWEHLEAEVNDLTGYPHILPTHQGR
                     AAEKILYGYLGGRGKVFISNTHFDTTRANIEFSGAEAIDIPCAESYDHNSDYPFKGNM
                     DTEKLDKLIVQHGAPNIGAIILTVTNNTGGGQPVSMANAKAVADICRKHGVLYIIDCC
                     RIAENSYFIRHKEAGFESTPY"
     gene            835..2217
                     /locus_tag="PLG24_00230"
     CDS             835..2217
                     /locus_tag="PLG24_00230"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HMZ23333.1"
                     /translation="MILKKRSWAEPFKIKMVELLKMTTPEQRRKAIREAGYNTFLLKS
                     EDVYIDLLTDSGTSAMSDRQWAGLMLGDEAYAGSQNFYNLEKSVHKYYGFKYLIPTHQ
                     GRGAENLLSQVLIKKGDIVPGNMYFTTTRLHQELAGGTFVDVIIDEAHDSESLHPFKG
                     DIDLNKLEDLIRKHGAGKIPYVCVAVTVNMAGGQPVSMNNLREVRKLTEKHGIKVMLD
                     MTRIAENAYFIQQREQGYADRSIASLVREICSYTDGATFSGKKDALVNIGGFLAVNDK
                     EIFEEASNLVVVYEGLHTYGGLAGRDMEAMAVGIKESVHDEHMKARIGQVIYLGEKLQ
                     EAGIPIVLPIGGHGIFIDAKRFLPHLTQDQFPAQALAAELYVDSGVRTMERGIVSAGR
                     NNKTGDHHYPKLELVRLTIPRRVYTQAHMDVIAESVEHVYQRRKKIKGLEMVYEPRYL
                     RFFQARFKKL"
     gene            2947..3141
                     /locus_tag="PLG24_00235"
     CDS             2947..3141
                     /locus_tag="PLG24_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23334.1"
                     /translation="MKPDKPGQVVKFPTKPPYNLKVVKVSKQKIIPDLTRGVKGVETN
                     VWLTIEEENGKEYTGTLFVN"
     gene            3152..3391
                     /locus_tag="PLG24_00240"
     CDS             3152..3391
                     /locus_tag="PLG24_00240"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ23335.1"
                     /translation="MPIDYTASESLIKDYRLKPGDKIRQVREERGYSQEQLADIMNIN
                     RSTISKIENGKFSVTVDYLVRFSILLDYEFKVIER"
     gene            3579..5192
                     /locus_tag="PLG24_00245"
     CDS             3579..5192
                     /locus_tag="PLG24_00245"
                     /inference="COORDINATES: protein motif:HMM:NF023524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3578 domain-containing protein"
                     /protein_id="HMZ23336.1"
                     /translation="MYRQNNKNKMNYYQELKKFLSQAKTAELSTIAYAKIFQGLSVKV
                     SFGKGNPARVPWIAFLNDIDNVQKGIYPVYLFYKDKNLLILAYGVSETYTPNRKWNIT
                     NVKTIEQYFTDHNLGEPERYGSSFVFNSYNVNEDIQEEKINTDLNELISIYKANSDES
                     SGVTELKVEFKHTAFLNLAKNAGYFIEEKFCIRFCASLLTKPFVILTGLSGSGKTKLA
                     QAFVQWICRNKSQYRIIPVGADWTNREPLLGYPNALKPEEYVKPDSGVLDLIIQANQQ
                     PDLPHFLILDEMNLSHVERYFADFLSVMESKEEIPLYAESTIENGVPAKLKVPSNLFI
                     IGTVNIDETTNMFSPKVLDRANTIEFRVTQDEMKKFLGNIRDINMDALTSKGAGMAKS
                     FLEMAASKEFATADITEINEALVQFFGELKKTGAEFGYRSATEILRLIHQLTVLDNTL
                     TTNQKIDIAIIQKLLPKLHGSRRKLCPVLETLGSFCITGNVSIIKDVFDNADFEYNGD
                     IVLYPLSLEKISRMYRGAIDNGFASFAEA"
     gene            5195..7483
                     /locus_tag="PLG24_00250"
     CDS             5195..7483
                     /locus_tag="PLG24_00250"
                     /inference="COORDINATES: protein motif:HMM:NF021352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2357 domain-containing protein"
                     /protein_id="HMZ23337.1"
                     /translation="MKKVANIEINLDSVQDGLRLWIDARRPDTLFDAEENASDHNEAR
                     YQLVEGCLYDFQISDPQYVLGDVGENIVQPHKRTPNLGTIAPNIFVGTLSIPLLEKET
                     LQECTKIDLEVQSVKSGYRDDYRDMLELITEKCTDLLLRANSPVSQHFEIDYTRDGQS
                     LYQKFAFIKSVIGTEEFSEAVHRIVTAPVTKWTETTEEKDIRNARRFSNANIKEILKG
                     SKRTRLPDSHYLKSYGIETLPERISTIRKSDSVDTPENRFIKHALEKFLKFCTDMNNK
                     AREYGHKKMENESEIMIRELESQLHHTVFKDISRPTTLKLNSPVLQRKEGYREVLRVW
                     LMFDLAAKLIWKGGDDIYSGGKKDIATLYEYWLFFKLLDLFQSIFEIEPKDISDLIEA
                     TPDGLNLQIKQGKFTALCGVYDSGSRKLNVRFNYNRSFSGKQTYPESGSWTTTLRPDY
                     TLSFWPHGISETEAEKQELIVHVHFDAKYKIANLADFLEQNNENDLDEEKAENRKGIY
                     KNADLLKMHAYKDAIRRTGGAYVLYPGDKAINQKGFHEIIPGLGAFPVKPSRTDSGIG
                     ELKAFILEIIEHFINRASQREKIAYRTFDVYKNPPKSDNVVKEPLPEPYDNNRDLIPD
                     DTFVLVGFYKSEAHLNWIKEKKLYNFRMDNARGALVLTKESLSSKYLLLHTSEDKSSG
                     ELWKIIGEGFRVISQEALKKLNYPSPSQRNYMIVKIEKVTDPEFKDVTWDFKNLSNFS
                     TGRASAFPFTTSLTELMKNKIR"
     gene            7870..8958
                     /locus_tag="PLG24_00255"
     CDS             7870..8958
                     /locus_tag="PLG24_00255"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HMZ23338.1"
                     /translation="MTRIYNTIGSLTTLQSLLEENRIYDFKSLEQIIDFRNSYFTSCK
                     QILSHHTDLIEEERIVLGAELKNLEAEIEKNQLEVELSLTGENEEIEKQIDSSVSQTS
                     VNLFQNITGKLKLWNFTRKFKNKENQFDFRIRIKMFHLLETFQKKKKHLNFINSHLDE
                     AVKQSASNALYELDRKKTVIDNLSSLIYGALGEQKVVRELENLPDEYILINDFSISIS
                     PPIYFRQEDDYIMSVQIDHVLVSPAGVFLIETKNWSKKSLENLSLRSPVDQIRRTSFV
                     LFLLLNNEMDDYHLQLDIHHWGDKKVPIKNLIVMINTKPQEEFKYVKVLSLSQLPGYI
                     RYFKPIFSNEDTQRIADFLLRINDSKAI"
     gene            complement(9671..10105)
                     /locus_tag="PLG24_00260"
     CDS             complement(9671..10105)
                     /locus_tag="PLG24_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23339.1"
                     /translation="MKNQNLTSGIPYIPKTWAALGFFIIFLLSLALFRGRKSEWIRYD
                     ALSALFTDFYSHISNFSISLDLCLIFGYLSVLKTGKLRLVYYFSALMILSNIVYEYFI
                     PILNTRDPLDALYGIIGVLFALIFSFFLQKSGLQTNPAYSPP"
     gene            complement(10182..10361)
                     /locus_tag="PLG24_00265"
     CDS             complement(10182..10361)
                     /locus_tag="PLG24_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HMZ23340.1"
                     /translation="MKAPPAFIERAKKSPKTYAFLNTLNRRNLFSIAFRLHTVKKPET
                     REKPDDKDPGYDGRR"
     gene            10670..11023
                     /gene="ssb"
                     /locus_tag="PLG24_00270"
     CDS             10670..11023
                     /gene="ssb"
                     /locus_tag="PLG24_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HMZ23341.1"
                     /translation="MTTLHNKVRLIGNVGQQPEIRTFDSGVKLARLSLATVDRRRDFT
                     GEYVSETTWHNLVAWGRHAEVIGKYVGKGREIMVGGTLINRSYMDKNGDKRYLSEIRI
                     SEIKLLGSPGDRPNQ"
     gene            11283..13796
                     /locus_tag="PLG24_00275"
     CDS             11283..13796
                     /locus_tag="PLG24_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HMZ23342.1"
                     /translation="MNTAIANNNFTMQDLEINDAFRLASQYVNETDEHVFITGNAGTG
                     KTSFLRHISQNCRKNLVIAAPTGIAAINAGGTTLHSLFQLPFAPFVPTDTNKAELAAS
                     IRISRAKLDIIRNMDILVIDEVSMVRADVLDAVDTILRSSRRQHNASFGGLQVLFIGD
                     LNQLPPVTKQEEWSLLSPYYDSPFFFDSQAVRFRPPVVIEFTKIYRQQNQDFISLLNK
                     VRNNNLDLAGYDTLNSRFIPDFKPPEGSNYITLTSHNLRAEQANQLKLDELPGKVHTF
                     YARIENDFPEYLFPNEQELRLKVGAQVMFIKNDVINRAYFNGKIGFVKEVNDDDVVVD
                     CEGIEIKVSADTWENNKYTVDPDSSTVRQECTGTFSQLPLRLAWAITIHKSQGLTFDR
                     VMIDAAASFSSGQVYVALSRCKTLEGIVLLSRIESRAILFDNRIDTGISRLNYEKRAQ
                     AYLQQARNNYILTIVGEMLGFHNEIRYCNFILSELRKFGDKFPTDSGEYWIGVKSTLA
                     EYQPIVDKFILSLEKLSKEDKQFEEKATLKGRIHDAIKYFEPRVAGLVRQVKANPTNT
                     ESREAASRVNELLNELFDALARKQHILSRCNTAFKLELYQEAKKTFNPRHEKLSVYNQ
                     SSDADDEGNHPLLYKRLRRWRDSIVQSNNIPVYMVGNSKMLQEIATLLPVEKKQLLNV
                     KGMGSAKVAKYGEEILDIVRDYCDEFDIEPAMPALTFEKKTKVDEPKKKVSEGKSIKQ
                     KTTEITYGLWRANPDINYLARLRNLTTGTIEHHLSLLIQEGKIKIEEFVPPDMIRLLT
                     PILARHEGKTLSEIKPFCPESVTFEQLRALREHLNQGKS"
     gene            13816..14379
                     /locus_tag="PLG24_00280"
     CDS             13816..14379
                     /locus_tag="PLG24_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HMZ23343.1"
                     /translation="MQNQSDNFLTSVIRLSEYYKSLGEKAMVQLEEEHLNLRLNEESN
                     SISTIVKHLWGNMLSRWTDFLNADGEKEWRERDAEFENDICGRHDIMAKWEEGWKCYL
                     DALRALSPDDLTKIVYIRNQGHTVQDAIHRQLAHYSYHVGQLVFLAKVLVNHPWESLS
                     IPRHGSIAYNAEKFSQEKRREHFTEDK"
     gene            complement(14754..15272)
                     /locus_tag="PLG24_00285"
     CDS             complement(14754..15272)
                     /locus_tag="PLG24_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HMZ23344.1"
                     /translation="MKIKNFEFKAKVSCIDDYESKLLAKGAVFIGTDHQIDTYFNTSR
                     GRLKLREGNIENALIHYEREDTASIKESRIILYKSKPDPALRDILILHMGVRVVVDKI
                     RKIYFIDNVKFHFDEVRGLGSFIEVEAIDEEGKFTRDELQRQCDAFFHFFALSESDLV
                     NQSYSDLLLNTA"
     gene            complement(15277..16581)
                     /locus_tag="PLG24_00290"
     CDS             complement(15277..16581)
                     /locus_tag="PLG24_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HMZ23345.1"
                     /translation="MRLLKYFPVFLLLLSCNPVGKQENPQSDFIRTEGIHFMQGDSTY
                     YFFGTNYWYGPALAMLEDTTRGIDRLRKELDFLKSHGVVNLRICAAVEGSGIIQGVPR
                     IFPVLQTAPGVFETANLRGLDRLLDEMKKREMRAVLFFSNNWEWSGGFLQYLYWNKMV
                     EEKDFVERMPWEQLRDVVSRFYSCEPCMDQYSAQVRTVLARTNSINGVKYTEDPTIFA
                     WQLANEPRPMRPAAIDAYKAWITRTAAMIKSLDSNHLVTIGVEGEIGTENMEVFKAIH
                     SDKNIDYGTIHIWPRNWAWYKDLNDEEQQAGSMQLTDDYIRKHIDAMREIGKPLVIEE
                     YGYPRDNNSFEPTSATTRRDGFYEKILLHWQESFAQKGVLAGCNFWAFGGTARPIDGQ
                     KFWKPGDNFMGDPPMEEQGLYSVFDSDTSTWKVIEKYIGRKQ"
     gene            17415..17930
                     /locus_tag="PLG24_00295"
     CDS             17415..17930
                     /locus_tag="PLG24_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23346.1"
                     /translation="MRHLLPLRSKSYNLKEFFKNTGLYSLVFLLYLSNTGLILTVHTC
                     IHTGISVVELPSFQTHDDVCSEVIGERSCAQDDNADDCCSREIEKKEDCHTHKTEYKK
                     ADYTSIKPEPNKYGTFTGVAEVSFIYYQILPLIPKEREEHHIIYHANKARDNTTESAL
                     EYRIGLASFLC"
     gene            18010..20232
                     /locus_tag="PLG24_00300"
     CDS             18010..20232
                     /locus_tag="PLG24_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ23347.1"
                     /translation="MNVKYLWIAVILTLSNLMYAQNEDGIRSKLIDQHGTPIIAAWVS
                     INHSATKVQTDENGYFLLKTSSWPAHLDIDMGDNNVVHVDIANEEEAKVITAKKVVEL
                     HEVVVKEKVTRELNTIQTRNVETITRKEFQKAACCRLSESFDNTGSVSVSETDAVTGA
                     KEMEILGLRGIYSLMTLDNIPDFCGINFPFALDMIPGTWLDEVSISKGISNAINGSYG
                     FSGQVNVGLKNPFEDKPLFVNLYGNTMGRYEANVHMNKVLPNPAFATGLYLHASKNFS
                     KIDHNHDNYLDMPISTQLNALYKLKINGLGPWESNLDVQFVRDDRKSGQKTVSAEHPY
                     TATSDAQRFTINGNTGFVGFDKEGRSIGFKYQYTNNILDAAYGGLNYAGTQNRGYFQA
                     LYEDRFDDARHIIYAGASILAEGLKQKIAGTDFNRNELVPGVYAEYAYNPEITDVNKK
                     FTERFGLVAAVRADFHNIYGTQIAPALTAKYNITPDMVLRANVGKGYRTPHFFTDNIS
                     SFVNGRPIQITENLDPEEALNYGLSYTLKSKIARRNFSINADLYQTRFQNQIIVDQET
                     DLSAVRVSNLNGESVTTSALLSTSYEILNDFTAKIAYKWNDIRYDQAGQQVTKILIPR
                     HRALLALHYTTPDKGWEFSNTTTWIGPQEYLERIQVEGGVAQIRHEAKSFVTINAQIT
                     KKWNDFDLYFGGENLTNYTQKNPIQGGDAPQSDLFDVSQVYAPLAGIRGYVGIRWSIL
                     "
     gene            complement(20487..21122)
                     /locus_tag="PLG24_00305"
     CDS             complement(20487..21122)
                     /locus_tag="PLG24_00305"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HMZ23348.1"
                     /translation="MRRNIALVLLGLLSFFSSNAQFIDDWEARPSVSIAYQVNKKLEV
                     EATYYLYLIDNLSRFEKSLFSGELEYEFAPWAVAGLDYRYGTGSGVDYHDLRYSIEFA
                     HPWKSGKWILVYRPTFQQKIKIGVDTDNYLRNKLELNFLLNPKVNLFIFTENYQLIER
                     GLSFNTQKNALGGIYSINRRHKLELQFTLKNKRDHKNYARLETAYVFKFGQ"
     gene            complement(21115..22062)
                     /locus_tag="PLG24_00310"
     CDS             complement(21115..22062)
                     /locus_tag="PLG24_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HMZ23349.1"
                     /translation="MLWIIFITFIIIALALDLGVFNKTPHIIKTKEATAWTLVWISLA
                     LLFSGIIYLAFEHQWVENPTSLTPYNAVLKYISGYLIEISLSVDNIFIIAVIFSSFSI
                     PQKYQHEVLFYGVLGAIVFRAIMIFFGVALITHFSWIIYVFGAFLIITAYRMLTHKSE
                     DFNPKKSKLFSLIKKIYPVTYMMDGDKFFIKKRGIKAATPLFVALVIIELTDLFFAID
                     SIPAILAISADPFIVFSSNILAVMGLRSMYFLISGMLNKFRYINYSLVVILAFVGVKM
                     ILSHHVEFPEWVSLGVIVASLTGGIMASLLIPEKKELSA"
     gene            complement(22074..22754)
                     /locus_tag="PLG24_00315"
     CDS             complement(22074..22754)
                     /locus_tag="PLG24_00315"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HMZ23350.1"
                     /translation="METLFELFANLIDPDWIMSQGGLYLVLVILFIETGLFFGFFLPG
                     DVLLFISGIILASADQTVHIFDHKVIDLVFWQSLFIGSTILGNFVGYWFGSKFGHALF
                     YQGRKFLFLRQKHIDAAQQFYEKRGGFAVAIARFLPIARTFVPIVAGMVKMNFKQFTF
                     YNVLGAFLWVGGLTTLGFVLGKREWVADNLEWAVLAVLVLASLPMIIKMIFPGKKQGA
                     KKEKIRIL"
     gene            23222..>24424
                     /locus_tag="PLG24_00320"
     CDS             23222..>24424
                     /locus_tag="PLG24_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HMZ23351.1"
                     /translation="MRNLPVFLSLICLFSINNLSGQSQIFKELQQREKAGTIVQKKIV
                     KNLLSEEGAEQVGLSKDGNAIYFNIDQPVIAEIIVHRPALLRVKIPSVSSGEELTLLL
                     FNKKPFAEGAVVTTSAGDRHPMSGGAYYRGTVEGDEHSVVALSFVDGEMVGVVSVNNR
                     QFDIGKVEHSDVHVIYDATKVNRKAPTGCEVKYVEGYKPDMIGEAQIETRAADKCVNI
                     YFEVSYSIYQNKGSVSAVENYVNGFFNVVSTLYGNENIQVVISEIFVWTSSDPFPTND
                     AGDALDYFTGYRTSFNGDIAQLLAYTDNGNGGIAWIDVLCNFGGAYSYSYSDIDMTYN
                     MLPNYSWTTNVVTHELGHNLGSEHTHWCGWQGGAIDNCYTTEGGCPPGPPPSNGGTLM
                     SYCHLTGYG"
BASE COUNT         7541 a         5054 c         5277 g         6552 t
ORIGIN      
        1 atacggagtg gattcgaatc cggcctcctt atgtcggata aaatacgaat tttcggcgat
       61 gcggcagcaa tcgatgatgt agagaacacc gtgcttcctg cagatatcgg caacggcctt
      121 agcattggcc atgctgaccg gttggccgcc acccgtattg ttggtcacag tcaggatgat
      181 ggcgccgata ttcggagcac cgtgttgtac gatcaattta tccagttttt ccgtgtccat
      241 attgcccttg aatgggtagt cggaattgtg atcatatgac tctgcacacg gaatgtctat
      301 cgcctctgct ccgctaaact cgatattggc acgggttgta tcgaaatgcg tgttgctgat
      361 gaacactttc cctctaccgc ccagatagcc gtacaaaatc ttttctgcag cacgtccctg
      421 atgcgtaggc aggatatggg gataccccgt cagatcattt acttcagctt caaggtgttc
      481 ccagctcagt gctcctgcgt atgattcgtc cccgcgcatg atgcctgccc actgatcact
      541 actcatcgcc gaagtgccgc tgtcggtcag caggtcgatg actacctgag aggaatgaag
      601 taaaaaaggg ttgaaatgag ccttgtggat attttcaatc ctttctgcct cggtagacat
      661 ccgtatgggc tctacagact tgattctgaa gggctctata atggtcttaa atttcatttt
      721 ttaggtttta agaaaaaatg acgcaaaggt cttaaaatta aactttacaa aattatgatt
      781 ataatcatga ttttagtgag tttgactcct ttcttttgcc aaaaatttaa agcaatgatc
      841 ttaaagaaaa gatcctgggc agagcccttc aagataaaaa tggtcgaact gttgaagatg
      901 accaccccgg aacagcgcag gaaagccatt cgcgaggccg gatataatac ctttttactc
      961 aaatcggaag atgtctatat tgacttgctt acggatagtg gaacatcagc catgagcgac
     1021 aggcagtggg cgggattgat gttgggtgat gaagcttatg cgggcagcca aaacttctac
     1081 aacctggaaa aatccgttca taaatactac ggtttcaaat acctgatccc aacacaccag
     1141 ggtcggggag ccgaaaacct tctgtcacag gtattgatca aaaagggtga cattgtgccg
     1201 ggcaatatgt atttcaccac aacccgacta catcaggagt tggcgggagg aacctttgtc
     1261 gatgtcatca ttgatgaggc acatgactca gaaagtctgc acccatttaa aggagatatt
     1321 gacctcaaca aactggagga tcttatccgg aagcatggag ccgggaagat cccttatgta
     1381 tgtgtcgcag taacggtcaa tatggctggc ggacaacctg tctccatgaa taacctcagg
     1441 gaggtgcgaa agctgactga aaaacacggc atcaaggtca tgctcgatat gacgaggatc
     1501 gcggagaatg cctactttat ccaacaaagg gagcaagggt atgccgacag gagcattgcc
     1561 tccctcgtca gggagatttg cagttacacg gacggcgcga ctttcagcgg aaagaaggac
     1621 gccctggtca atattggtgg cttcctggca gtcaatgaca aggagatatt cgaagaggca
     1681 agcaatctgg ttgtggtgta tgaaggtctg catacctacg gcggccttgc cgggagggat
     1741 atggaagcca tggctgtcgg aatcaaagag tcagtccacg atgaacatat gaaggcccga
     1801 atcgggcagg tgatatatct cggcgaaaaa ttacaggaag caggaatccc gatcgtattg
     1861 ccgattggag gacacggcat cttcatcgat gccaaacgct ttcttccgca cctgacgcag
     1921 gatcagtttc ctgcacaggc tctggctgcg gaattgtacg ttgattcagg tgtgcgcact
     1981 atggagagag gcatcgtttc agcaggaagg aataacaaga caggagacca tcactatccg
     2041 aaactcgaac tggtgcggct tacaatccca cggagagtct atacccaggc ccacatggat
     2101 gtaattgccg aatcggtgga gcatgtctat cagaggcgca agaagattaa aggccttgaa
     2161 atggtatacg aaccaaggta tctgcgcttc ttccaggcga gattcaaaaa gttatgagga
     2221 gactgaaggg caagtctctt cccttcagtt ggctgcatca gaggattatt gaaggtcgaa
     2281 atcaggcgac gttatcacgc tgctgtattg actaaataat ggattgaata tccacctccc
     2341 ggaatgaaat agcctgttgg tccggaaacg accaatgtcc ggatatgaca cctgttcagg
     2401 aaggcttcat aacaatccgg tgacaggcct tcaattcccg gtaattattc acccgaattt
     2461 gctacaaggt aatatggtct gaaaggattt cgtttttttt cggttcttcc caatattgac
     2521 caatcatggg ttagattccc gggtaagggg ttattatgga ccttgagtcg acccatcgtg
     2581 acggttgaaa ttcgggtttg accggcattg tcgcaagagt caggaattcg gtatccagtt
     2641 tacccgtgct aacgatatta gttttgtaaa cgatattggc ggggcaatta tggcgctgtg
     2701 tgccactgta aaatgccagc cgcacaaaca gccacagtag gatttaccac catacaagcc
     2761 agggtacaat aaaccggaag tgcctgattt ttattgatgt aacgaacgac agcaagttaa
     2821 cagacattga caggtatgtt gaaaaaaggt gttcaatata ttacactttt ttaaaataag
     2881 aaaattatct ttgaaccatc aataattaac agcgggggca acgctataaa ttctgcacac
     2941 tggcaaatga agcccgacaa accgggacaa gtggtaaagt ttcccacaaa gccaccgtat
     3001 aaccttaaag ttgtaaaagt tagcaagcag aaaattattc ccgaccttac ccggggagtt
     3061 aaaggagttg aaacaaatgt ttggctgacg atagaggaag agaacggcaa agaatacacc
     3121 ggaaccttat ttgtaaatta attacaatct catgcctata gattatacag catcagaatc
     3181 actgataaaa gattaccgtc tgaagccggg tgataagatc aggcaggtgc gtgaagagcg
     3241 tggttacagc caggagcagc ttgcagatat aatgaacatc aaccgttcca ctatttctaa
     3301 aattgaaaac ggtaagttca gtgttacggt agattacctg gtgcgttttt ctattctcct
     3361 ggattatgaa tttaaagtaa ttgaaaggta gcagatggca gatgtacacg acaaagctac
     3421 acgtagctac aacatgcgta gaattaaagg caggaataca aagccggaaa tgcttgtacg
     3481 gaaatacctt catgcaaaca gactacgata ctggttacac actaataatc tgccaggaaa
     3541 aattgaatgt ttaaggcata ataaggtacc atctatccat ctatagacaa aataataaaa
     3601 acaaaatgaa ttattatcag gagttaaaaa aatttctttc gcaagctaaa accgcagaac
     3661 taagcacaat cgcttatgca aaaatttttc aaggcttatc agtaaaagta agctttggga
     3721 aagggaatcc agcaagagtt ccttggatag cttttctaaa tgatattgat aatgttcaga
     3781 agggaattta tcctgtttat cttttttaca aggataaaaa cttattaata cttgcttatg
     3841 gtgtaagtga gacatataca ccaaatagaa aatggaatat aactaatgtt aaaacaatcg
     3901 aacaatactt tactgatcac aacttagggg aaccagagag gtacgggagc tcattcgtct
     3961 ttaatagtta taacgttaac gaagacattc aagaagagaa aataaatacc gatttgaatg
     4021 aattaatatc aatttacaag gcaaattctg atgaatcgag tggagtaact gaactaaagg
     4081 tagaatttaa gcatacagcc tttcttaact tagctaaaaa tgcaggttat tttatagaag
     4141 aaaaattttg tatccgtttt tgtgcctccc tcctcactaa acctttcgta atacttaccg
     4201 gcctttcagg atcaggcaaa accaaactgg cacaggcctt tgtgcagtgg atctgtcgga
     4261 acaaaagtca gtaccgtatt attccggtag gtgccgattg gaccaaccgc gagcctttgc
     4321 tgggttatcc caatgcactg aaaccggagg aatatgtaaa gcccgacagc ggagtactgg
     4381 atctgattat tcaggccaac caacagcccg acctaccgca ttttctgatt ctggatgaaa
     4441 tgaatctgag tcatgtagaa cgatattttg ctgactttct cagcgtaatg gagtcgaagg
     4501 aagaaatacc actgtatgca gaaagtacta ttgaaaacgg agttccggca aaactaaagg
     4561 ttccttcaaa tcttttcatc attggcacgg taaatataga tgaaaccacc aatatgttca
     4621 gtccaaaagt attggacaga gccaatacca ttgaattccg tgttacgcag gatgaaatga
     4681 aaaaatttct gggcaatatc agagacatta acatggatgc ccttaccagc aaaggtgcag
     4741 gcatggccaa aagctttttg gaaatggccg ccagtaaaga atttgctact gctgatatta
     4801 ccgaaatcaa tgaggctctg gttcaatttt ttggcgagct gaagaaaacc ggagcagaat
     4861 ttggctatcg cagtgcaaca gaaatccttc gcctgattca tcagcttact gttttggaca
     4921 atacgcttac aactaatcaa aagattgata tcgccatcat tcaaaagctg ttgcctaagc
     4981 tccatggttc acgcagaaag ttgtgtccgg tactggaaac gttgggttcg ttctgcatta
     5041 cgggtaatgt aagcataatt aaggacgtgt ttgacaatgc agactttgag tacaatggtg
     5101 acattgtact ttacccgctt tcactcgaga aaatatcccg gatgtaccgc ggtgcaatag
     5161 acaatggatt tgcaagtttt gccgaggcat aaaaatgaag aaagtagcaa acatagaaat
     5221 caacctggat tcagtgcagg atggtttgcg actttggatt gacgcccgca gaccggacac
     5281 attgtttgat gcagaggaaa atgcttctga tcataatgaa gctcgttatc agctggtgga
     5341 aggttgtttg tatgatttcc agatcagcga ccctcagtat gtgttaggcg atgtaggtga
     5401 aaacattgta caaccacata aacgaactcc aaaccttgga accattgctc caaatatttt
     5461 tgtaggaacg ctttctatcc ccttacttga aaaagaaacg ctacaggaat gtacaaaaat
     5521 tgatctggaa gttcagtccg taaaatcagg ctatcgggat gactaccgcg atatgctgga
     5581 actgataaca gaaaaatgca ccgacctgtt gttgcgggcc aattcacccg tttcgcaaca
     5641 ttttgaaatt gattatacca gagacggtca gtctctttat cagaaattcg cctttatcaa
     5701 atcggtaatc ggcactgaag agttttcaga ggctgttcac cgtatagtaa ccgctccggt
     5761 aacaaaatgg acggaaacta cagaggaaaa ggacattcgc aatgccagac gattttcaaa
     5821 cgcaaacatt aaggaaatat taaaaggcag taaacgaacc cgattacctg attcgcacta
     5881 tctgaaaagt tatggtattg aaacattgcc cgaacgaatt tccacaataa gaaaatcaga
     5941 ttctgttgat acacccgaaa accgctttat aaaacatgcc cttgaaaaat ttctgaagtt
     6001 ttgtacagat atgaataata aggccagaga atacggccat aagaaaatgg aaaacgaatc
     6061 tgagatcatg attcgtgaac ttgaaagtca gttgcaccat accgttttca aagacatttc
     6121 aagaccaaca accttgaaac ttaatagtcc ggttttgcag cgtaaagaag gataccggga
     6181 agtacttcgg gtatggctca tgtttgacct tgcagcgaaa ctcatctgga aaggcggaga
     6241 cgatatatac agcggaggga aaaaagatat tgccaccttg tatgaatact ggcttttctt
     6301 caaactgctc gacctgtttc aatccatatt tgaaattgaa cccaaagaca tttcagactt
     6361 aattgaggcc actcccgacg gattaaatct tcagataaag cagggaaagt ttactgcctt
     6421 gtgcggtgta tatgattcag gaagcagaaa actgaatgtc agatttaatt ataaccgttc
     6481 tttcagcgga aaacaaactt atcctgaatc aggaagctgg acaacaacct taaggcctga
     6541 ttatacactt tcattctggc ctcatgggat ttcagaaacc gaagcagaaa agcaggaatt
     6601 gattgtgcat gttcattttg atgcaaaata taaaattgcc aacctggctg actttctgga
     6661 acaaaacaat gaaaacgatc ttgacgaaga aaaagcagaa aacagaaaag gtatatacaa
     6721 aaatgccgac ctgctaaaga tgcatgcata caaggatgcc atcagaagga caggcggcgc
     6781 ttatgtttta taccccggag acaaagcaat caatcaaaag ggctttcatg aaattattcc
     6841 gggtttgggg gcgtttccgg tgaaaccatc caggacagac agcggaatcg gagaactaaa
     6901 ggcattcatt cttgagataa tcgaacactt cataaaccgt gcttcccagc gtgaaaaaat
     6961 tgcatacaga actttcgatg tttataaaaa tccgccaaaa tctgacaatg tggttaagga
     7021 gccattacct gaaccatatg ataacaacag agacttaatt ccggacgaca cttttgtttt
     7081 ggtgggtttt tataaatcag aagcacattt aaactggata aaagagaaaa agctttacaa
     7141 ctttagaatg gataatgcac gtggagccct ggttttgact aaagagtccc tttcatctaa
     7201 atatttatta ttgcatacat cggaagataa aagtagtggt gagctgtgga agattattgg
     7261 agagggattt agagttataa gtcaggaagc tttgaaaaaa ttgaactatc cctcaccgag
     7321 ccaaagaaat tatatgatag tgaaaattga aaaagttact gatccggaat ttaaagatgt
     7381 cacctgggat ttcaaaaacc tttccaattt ttcgacagga agagcatctg catttccatt
     7441 tacaacaagt cttacagaat taatgaagaa taaaatcaga taacatgcct acccacctta
     7501 gtcaaacaaa ttaccaggcc ccgcaagcca aagtttcaac caggacctgg caagagtgtg
     7561 tctgtttcgc aaaaaaaatt tcccgactat tttgaaaata caaatacgca atcacacaga
     7621 ccgacgacag tcaataatca gttgctatgc agacaccaaa accgttgaca ggaaagcttt
     7681 ttaactgccc ggcagccagc cggataatcc gggttatact gacacttcat aggagattaa
     7741 ggaaagaaag ccaatttgca ggttattatc ataagggata ttccggaaaa aaatgagcca
     7801 ccttccacaa cgtcctgaaa aaaacaaaca atacatttag aaatcatgta ataaaacacc
     7861 gcaaacaata tgactaggat atataacaca attggttcac tgacaacttt acaatctcta
     7921 ttggaggaaa atagaattta tgatttcaaa tcattagaac aaattattga ctttcgaaac
     7981 tcttatttta cttcttgcaa gcaaatactt tcccatcata cggatcttat cgaggaagaa
     8041 agaatagtgc tcggggctga attaaaaaat ttagaagcag aaattgagaa aaatcaactt
     8101 gaggtagaac tatcactgac aggcgaaaat gaagaaattg aaaaacaaat tgattcttca
     8161 gtaagccaga cttctgttaa tttatttcag aatataaccg ggaaattaaa attgtggaac
     8221 tttacacgga aattcaaaaa caaagaaaat caatttgatt tccgaatacg aataaaaatg
     8281 tttcaccttc ttgaaacttt ccaaaaaaag aagaaacatc taaattttat aaattcacat
     8341 ttagatgaag ccgtcaaaca gagtgcttct aatgctctat acgaacttga tagaaaaaag
     8401 acggttatcg acaacctaag cagtcttatt tacggagctt taggagaaca aaaagtagtc
     8461 agggaactgg aaaatcttcc ggatgaatat attttgatca atgacttttc gatttcaatt
     8521 tctccgccaa tttatttcag gcaggaagat gattacataa tgtccgttca aatagatcat
     8581 gtacttgtat ctcctgccgg ggtcttcctg atagagacta aaaactggag taaaaaatca
     8641 ttggaaaatt taagcttacg ttcacctgtt gaccagatac gcagaacaag ttttgtttta
     8701 tttctcctgc tcaacaatga aatggatgac tatcatctgc aattggacat ccatcattgg
     8761 ggtgacaaaa aagtgccaat aaaaaatctc attgttatga taaatacaaa acctcaagaa
     8821 gaatttaaat atgtaaaagt cttatcctta agccagcttc ccggatatat caggtatttt
     8881 aaacccatat tttcaaatga agatactcaa agaattgctg acttcctctt aagaataaat
     8941 gatagtaaag caatctaaat caaatgatga caattaatct tatagcctga cagtttaaaa
     9001 tgagcaaaca ccataaggag aattgaacat ataatttagt aggtcagttt tgtttgatac
     9061 aaatctgcgc actattcaga tctctggttt aaaacaaaaa attgacagaa tttcatttgg
     9121 atttcagacc aacccggttg ctgcctactt tatccggatt gatggatata ccaagcttac
     9181 atggattttt ttcgtatgag atatttacat gacgaaccag gactgcgaga tcggtgtcat
     9241 ttcaatcagg gatcacatac tatcaggatc agacagctat ataaataaaa tccgggcatg
     9301 gaaactaaca gaccttcccg agcctgaaat gattgaattg gatggtggtt ttaacataac
     9361 actgttcaaa gcttggatat tccggcataa gttgcccccc ccaatttagc agaaaagaaa
     9421 tctcagaaac gagttataat ctttcaacat tctgacattc cggaataagc tgacccccct
     9481 gaacgggttt caataagcat gaggcaaatt ccggagcatg ttgaccccct ttcttgtgaa
     9541 atttcaaaga aaatgtggat tgatggtaaa caaccgccta agtacatctt ttttcaagtc
     9601 atcggtggtt ataccatatg gaaaaataat aatcttgact aagtacgaaa ttgcgaaaca
     9661 atatccgaag tcagggcggg gaatatgcgg gattggtttg cagtccggat ttttgtaaaa
     9721 agaatgaaaa aatcagggca aacagaacgc cgattattcc atataaggcg tctaagggat
     9781 cacgggtatt cagtataggg ataaagtatt cgtaaacgat attggaaaga atcatgaggg
     9841 ctgaaaagta atagacaagt cttaatttgc ccgtttttaa aacagataga tagccgaaga
     9901 tcagacacag atccaatgag atagaaaagt tggatatatg gctataaaaa tcagtaaata
     9961 aggctgataa agcgtcatac cttatccatt cggattttct cccccggaaa agcgctaggg
    10021 aaagtaaaaa aatgataaaa aatcccaatg ctgcccatgt ttttgggata taagggatac
    10081 cactggtcaa attttgattt ttcacggata attacaaatt ccagatcgta aaaataagat
    10141 aataaaacta agacgggtga acatactctt attgtagatt ttcaccttct tccatcatat
    10201 ccgggatctt tatcatccgg tttttcacgg gtctcaggtt ttttgacagt gtgtagtctg
    10261 aaggcaatgg agaagaggtt tcttctgttg agcgtattca aaaatgcata ggtctttgga
    10321 gattttttgg ctctttctat aaaagcaggt ggtgctttca tgacacgaca ccgttccaaa
    10381 atccgccatt acacaaggcg ccagaccgtt aaaggtattc acctcgtaaa tgtcttttgg
    10441 gtaaggcaac tattctgtaa aatgattgta aagttcaaat tatccgtttg aaaacgctta
    10501 caatcactta caaacgacta caaatgttta ataaccgaat atttatcggc cggtccttca
    10561 tttttgcctt gtcaatcgga atgggcaact tcaggaagat cctggcgggt ttgcttcatc
    10621 gaaaacaaat gtttgcgcga tatcattaac ttttttaata atacacatta tgacaacatt
    10681 acacaacaag gtccggctga tcggaaacgt aggtcagcaa cccgaaatca gaacttttga
    10741 ctccggcgta aaactggccc ggctaagcct cgccaccgtt gatcggcgca gagacttcac
    10801 cggtgaatac gtgtcggaga cgacctggca taaccttgtc gcctgggggc gtcatgccga
    10861 agtaatcgga aaatatgtag gcaagggcag ggaaatcatg gtcggcggca ccttgatcaa
    10921 ccggtcttat atggacaaaa atggtgacaa acgctatctg tcggaaatcc ggatatccga
    10981 aatcaaatta ttgggcagtc ccggtgacag acctaatcag taaagggccc taaggtggga
    11041 cttcgatgcc gcattcccgg attgcctttg tcgaacaata atcgttattt tcatcaaccg
    11101 aaatctttta tcatggtgct ttaggatgga ttcaatccga tattttgatc gcatggatct
    11161 gtacttcttt gcttgtgccc gaattggtaa attgtgagac gaaacaggtt gatgagcaat
    11221 aaaaacccca ctgtcttgaa aactttcaat atttttgaat ctaccaaaat cagtttccaa
    11281 ctatgaacac tgccatcgcc aataataatt ttactatgca ggatcttgag atcaacgatg
    11341 ccttccggct tgcaagccaa tatgtcaatg agacggatga acacgtcttc atcaccggta
    11401 atgcaggcac cggcaagact tcatttttaa ggcatatcag tcaaaactgc cgaaaaaatc
    11461 tggtcatcgc ggcaccgacg ggcatcgctg ctatcaatgc cggtggaaca acactccaca
    11521 gcctgtttca gcttccattt gccccttttg tgccgaccga taccaacaaa gccgagctgg
    11581 ctgcttccat ccgaatcagc cgtgccaagc ttgatatcat acgcaatatg gacatcctcg
    11641 tcattgacga ggtgtccatg gtgcgggcag atgtcctgga tgccgtggac accatcctgc
    11701 gaagcagcag gagacagcac aatgcctcct ttggaggact gcaggttctg ttcataggtg
    11761 atttaaacca gctacctccg gtaaccaagc aggaagagtg gagtttgctg tccccctatt
    11821 acgattctcc atttttcttc gattctcagg ctgtcaggtt taggccacct gtagtcattg
    11881 aatttacgaa gatttaccga cagcaaaatc aagacttcat cagcctgctc aataaggttc
    11941 gaaacaataa cctggatctc gccggctatg acacgctcaa tagtcgtttt attccggatt
    12001 tcaaaccgcc ggaaggcagt aactacataa ccttaacctc ccataacctg cgtgcagagc
    12061 aggccaatca attgaaactg gatgagctcc cgggcaaagt tcacaccttc tatgcccgaa
    12121 tagaaaatga ctttccggag tacttgtttc ccaatgagca ggaactaaga ctcaaggttg
    12181 gtgctcaggt tatgtttatc aaaaatgatg tcatcaatcg tgcctatttc aacggaaaaa
    12241 tcggctttgt gaaagaagtc aatgatgatg atgtcgtagt ggattgtgaa ggtatagaaa
    12301 taaaggttag tgctgatacc tgggaaaaca ataaatacac cgtggatccc gactcttcta
    12361 ctgtacggca ggaatgcacc gggaccttca gccagctacc tctcaggctt gcctgggcca
    12421 tcacgatcca taaaagtcag ggcctgacct tcgaccgggt aatgatcgat gcagcagcat
    12481 cattttccag tggtcaggtc tatgtggctc tcagtcgatg caaaacactg gaaggcattg
    12541 tcctgctttc cagaattgag tcgcgcgcca tcctcttcga caaccgcatc gataccggta
    12601 tcagtagact caactatgaa aaacgggctc aggcatacct acaacaagct cgtaataatt
    12661 atattcttac catagtggga gaaatgctcg gcttccataa tgaaatcaga tattgtaatt
    12721 tcatcctctc cgaattgcgt aagtttggtg acaagtttcc aacagattcc ggcgaatatt
    12781 ggattggggt aaaaagtaca ctggcagaat atcagccgat tgtagataag tttattctta
    12841 gcctggagaa gctatcaaag gaagacaagc agtttgaaga aaaggcaaca ctgaaaggac
    12901 gtatccatga tgccataaaa tattttgagc caagggttgc agggctggtt cggcaggtaa
    12961 aggccaatcc aacgaatacc gaatccaggg aagcagcttc gagagtcaat gaattactga
    13021 atgaattgtt tgacgcactt gctcgtaagc agcacatcct gagtcgctgt aacactgcat
    13081 tcaagctcga attatatcag gaggctaaaa aaacattcaa tccccgacat gaaaaacttt
    13141 cggtgtataa ccagtcatcc gatgcggatg atgaaggcaa tcaccctttg ttatacaaac
    13201 gactacgacg ctggcgggat agtattgtgc aaagcaacaa cattcctgta tacatggtgg
    13261 gcaacagtaa aatgctgcag gagatagcca ctcttctgcc ggtcgaaaaa aaacagctac
    13321 ttaatgtgaa gggcatggga agtgccaaag tggcaaaata cggagaagaa atattggaca
    13381 tcgtccgcga ttattgtgat gaatttgata tagaaccagc gatgcccgct ttgactttcg
    13441 aaaagaaaac caaagttgac gaacctaaaa agaaggtctc cgaaggtaaa tccatcaaac
    13501 aaaaaacgac ggaaatcacc tatggattat ggcgtgccaa tccggatatt aactaccttg
    13561 ccaggttgag gaacctgact accgggacca ttgagcatca tctgtccctt ttgatacagg
    13621 aaggaaaaat caagatagaa gaattcgtcc ccccggatat gattcgattg cttaccccta
    13681 tccttgccag gcacgaaggg aaaacgctct ccgagatcaa accattctgt ccggaaagtg
    13741 ttacttttga gcaattgcgt gcattgagag aacaccttaa tcaagggaaa tcatgaacct
    13801 aaaacgtttt atcagatgca aaatcaaagt gataactttc ttacttcggt catcagactt
    13861 tctgaatact ataaatctct gggggagaag gccatggttc aactggaaga agaacatctc
    13921 aacctcaggc tcaatgagga gagcaacagc atatcaacta tcgtcaagca tctttggggc
    13981 aatatgcttt cacgctggac ggattttctg aatgctgacg gagaaaagga atggcgcgaa
    14041 cgtgacgcag agtttgaaaa tgacatttgc ggcaggcacg atatcatggc caaatgggag
    14101 gagggctgga aatgttatct tgatgcactc cgggcacttt ctccggatga tctgaccaaa
    14161 attgtatata tccgcaatca gggccatacg gttcaggatg ctatccatcg tcagcttgca
    14221 cactattcct atcatgtggg tcagcttgta tttctggcca aagtcctggt caatcacccg
    14281 tgggaatctc tcagcatacc acggcacgga tccatagcct acaacgctga aaaattctct
    14341 caggaaaaga gaagagaaca ttttacggag gataaatagg gagttgcttc aaggacggca
    14401 cataacgaaa gatttttaca ggctgtaaat agcgacacaa accaagatac cgtttttctt
    14461 attttagttt acaaggctaa aactaataac gactgtaacc tcggcatccc gaatcccgga
    14521 ttgtttcact cccgggacag agcagagctc tggtagcata gttatccgga caagcatacc
    14581 tcaaatttat ggactattat ataaatcatc ctggtataaa atcgcactaa tccggctaaa
    14641 agcaggaacg gagattaggt catcggaaga tttgtctgat acaggttttg atcgtaaagg
    14701 aagcatcccc tttcatggat agacgatgaa gattactgat tattaggttc acgtcatgct
    14761 gtattcaaca acaggtcact gtatgattga ttgaccaggt cactttcact aagggcgaaa
    14821 aaatgaaaaa aggcgtcaca ttgtctctgc aactcatcac gggtgaattt gccctcttcg
    14881 tcaatcgcct caacctcgat gaaggagccc agtcctctga cctcatcaaa atgaaatttg
    14941 acattatcaa taaagtagat ttttcggatt ttatccacga ctacccggac gcccatatga
    15001 aggatcagga tgtccctgag tgccggatcc ggcttgcttt tgtacaggat gatccgggat
    15061 tccttgatcg aagcggtatc ttccctttcg tagtggatca gggcattttc tatgttacct
    15121 tctcttagct tgagccttcc gcgagacgta ttgaaatagg tatcgatctg gtgatctgta
    15181 cctataaaca ctgccccctt tgccagcaat ttggattcat aatcgtcaat gcacgaaact
    15241 tttgccttga actcaaagtt cttgatcttc ataaggttat tgtttcctgc cgatatattt
    15301 ttcgattacc ttccaggttg atgtatcgct gtcaaatacc gaatagaggc cttgctcctc
    15361 catcggtggg tcgcccataa aattatcgcc cggcttccag aatttctgac catcgatagg
    15421 cctggcggtg cctccgaaag cccagaaatt acatcctgcc agaacgcctt tttgcgcaaa
    15481 ggattcttgc caatgcagca atattttctc atagaatccg tctcgtcttg ttgtggcgga
    15541 agtgggttca aaactgttgt tatcccttgg gtagccatat tcttctatta caaggggctt
    15601 ccctatctcc ctcatggcat ctatgtgctt tcggatataa tcatcggtaa gctgcatgga
    15661 tccggcttgt tgctcttcgt cgttcagatc cttgtaccag gcccagttgc gaggccagat
    15721 atggatcgtg ccataatcga tgttcttgtc ggaatggatg gccttgaaaa cttccatgtt
    15781 ttcggttccg atctcaccct ccacacctat agtaaccagg tggttgctat ccagtgattt
    15841 gatcatggca gcagtacggg tgatccatgc cttgtaggcg tcaatggctg cagggcgcat
    15901 cggacgtggt tcgttggcaa gttgccaggc aaagatcgtt ggatcttccg tatattttac
    15961 gccattgata ctgttggtac gagccaggac ggttcgtact tgtgccgaat attggtccat
    16021 acacggctca caagaataaa atcgactgac aacatcgcgc agttgttccc agggcatcct
    16081 ttccacaaag tccttttcct caaccatttt attccaataa agatactgta gaaagccacc
    16141 actccactcc cagttgttgc tgaaaaacag tactgcccgc atctcccttt tcttcatttc
    16201 gtcgagcagc ctatcgaggc cccggagatt ggcggtttca aacacacccg gagcggtctg
    16261 cagtaccgga aatatccgcg gtacgccctg gatgatgcca ctaccctcca cagcagcaca
    16321 aatacggaga ttgactactc catgtgactt gagaaagtcg agctcttttc gtagcctgtc
    16381 gattcctctt gttgtatctt caagcatggc taaagcaggc ccataccaat aattcgttcc
    16441 gaaaaaataa taggtggaat ccccttgcat gaagtgaatg ccctctgttc ttatgaagtc
    16501 ggattgaggg ttttcctgct ttccgaccgg attgcaggac aataacaaaa ggaaaacggg
    16561 gaagtattta aggagtctca tgtgctgaca gattaaattt caaatgccaa agtttgtcaa
    16621 aatatttggc gtgggcaata ggctgccggt atttatcgga ctgtattttt gattattgaa
    16681 aatatttccc ggattcaatg catgacatta ccctccaaat aattctttac ctcactttaa
    16741 cctggcaaga tctttgttat caggtattga tttatctcaa tgcatagctt gaaaattttc
    16801 gaggcagctc aaattttcag tgataagaca aacattgagc gatggtcagc aaatgttttt
    16861 tggaacttca acggagggta aaagaagtgt aaatattcat tttaaattgg cttttcccgg
    16921 attaatatcg tcaacataag actaatttgt ctatacaatc cgggtggttt tcctaagcct
    16981 ttctataggc ggtatcagca tcagaaaagg accttatgcg gcttcaatga agttttgagg
    17041 ccccgcaaaa ggagttttgg atgtaatatg cctcgaaagg gtacgaaaaa aacaaaacgg
    17101 ccatgtgctc cggcagtgta tttcctgtta aaaaaaataa attaccattt ttggatcaaa
    17161 gacacatccg ttatgcaaag acaattggtt tattaaagag aaattcattg tttgatttga
    17221 aatgaagtga atatcttcct gttccgtcgg aacgtcatgc aggatgtgga agcgttgtac
    17281 atgcagtgag aaagtcttta tgatttcagg caaaggtggt ggatacgccg gtaattattt
    17341 gctttcgctt atccaatgac atagcgctta accggtaaac gctcaaacat taataaattt
    17401 ttaacaataa gagaatgagg catttactac ctttgcggtc gaaaagctat aacttgaagg
    17461 aatttttcaa aaataccggc ttatattctc ttgtgttttt gctgtatctg agcaacacgg
    17521 gattgatact tacagtgcat acatgcattc ataccggtat ttccgttgtc gaattacctt
    17581 catttcagac tcatgatgat gtgtgttcag aagtcatcgg tgagaggtct tgtgctcagg
    17641 atgacaatgc ggatgactgc tgttccaggg aaatagagaa aaaggaagat tgtcatacac
    17701 ataagacgga gtataaaaaa gcagactaca cctccatcaa accggaaccc aataaatatg
    17761 ggacttttac cggtgtagcc gaagtttctt ttatttatta ccagattctc cctttgattc
    17821 caaaagaaag agaggaacac catatcatct atcacgcaaa taaagccaga gataatacta
    17881 ctgagtcggc cctcgaatac cggatagggc tcgcctcctt cctttgttag catattgttg
    17941 gttcttcacc cagttagtga ttgcacgggc ttcggctctt gccatatact caaccaataa
    18001 tatcaaaaaa tgaatgtaaa atatttatgg atagctgtta tcctgacact ttccaatctt
    18061 atgtatgccc agaatgaaga cggaattcgc tccaagctga tcgaccaaca cggaactccc
    18121 attattgccg cttgggtaag catcaatcat agtgccacta aggttcagac tgacgaaaac
    18181 ggttacttcc tgttgaaaac atcttcctgg ccggcacatc tggatatcga catgggggat
    18241 aacaatgtag tgcatgtcga tatcgccaat gaggaagaag caaaggtcat tactgctaag
    18301 aaagtggtcg aattgcacga agtggtggtt aaggaaaaag ttacccgcga attgaatacc
    18361 atccaaacca gaaatgtcga aaccatcacc agaaaagaat ttcaaaaggc agcatgttgt
    18421 cgactcagtg agagctttga caatacgggt tcagtcagcg taagtgaaac cgatgccgtg
    18481 accggtgcca aggagatgga gatactcgga ttgcgtggca tatacagcct gatgactttg
    18541 gacaacattc ctgatttctg cgggatcaac tttccatttg ccctggacat gatacccggt
    18601 acctggttgg atgaggtgtc catctccaag ggaatctcca acgcaatcaa cggcagttat
    18661 ggattttccg gacaggtcaa cgttggtttg aagaatcctt ttgaagacaa gcctctcttt
    18721 gtcaacttgt atggcaatac aatgggtcgc tatgaagcca atgtccatat gaacaaagtg
    18781 cttcccaatc cggcattcgc cacaggactg tacctgcatg ccagcaagaa tttttcaaag
    18841 attgatcaca accacgacaa ctacctggac atgcccatat cgactcagct gaatgctttg
    18901 tacaaactaa aaataaacgg actgggtccc tgggaaagca acttggatgt acagtttgtc
    18961 agggacgaca gaaagtccgg acagaaaact gtcagtgctg agcatcctta tacggccaca
    19021 agtgacgccc agcgatttac catcaatgga aacacgggtt ttgttggatt cgacaaggag
    19081 ggtaggagca taggattcaa gtatcaatat accaacaata tcctggacgc agcctatgga
    19141 ggtctaaact atgccggcac tcaaaaccgc ggctacttcc aggcattgta cgaagaccgg
    19201 tttgatgatg caaggcatat catatatgcg ggtgcaagca ttcttgcaga aggtcttaaa
    19261 cagaaaatag ccggtaccga tttcaacaga aacgaattgg tgccaggagt gtatgccgaa
    19321 tatgcataca acccggaaat taccgatgtc aataaaaaat ttacggaacg atttggactt
    19381 gtagcagcag tcagagcgga ttttcataat atctacggaa cacagattgc tcccgcactt
    19441 accgccaaat acaacattac accggatatg gtattgcgtg ccaatgtagg caaaggctat
    19501 cgcactccgc acttctttac agacaacatt tcctcctttg tcaatggaag gcctatacag
    19561 attactgaaa acctcgatcc cgaagaagca cttaactatg gtctgtccta tacactaaag
    19621 tcgaagattg cgcgcaggaa tttctccatc aatgccgatc tttatcagac acgattccag
    19681 aatcaaatca tcgtggatca ggaaactgat ctttctgcgg tacgagtctc taatctcaac
    19741 ggagagtcgg tcacgacttc tgctctgttg agtacgagtt acgagatttt gaatgatttt
    19801 accgccaaga tcgcgtacaa atggaatgac ataagatatg accaggctgg acaacaagtg
    19861 acaaaaatcc ttattcccag gcaccgcgcc ttactggcat tgcactatac tacaccggat
    19921 aagggttggg agtttagcaa caccacgaca tggatcggcc cacaggaata tctggaaaga
    19981 atacaagtgg aaggaggcgt ggcacagatc cgacatgagg cgaaatcttt cgtgaccatt
    20041 aatgctcaga taacaaagaa gtggaatgat tttgacttgt attttggtgg agaaaacctg
    20101 accaattaca cccaaaagaa tccgatacag ggaggagacg ctccgcagtc agacttgttt
    20161 gacgtaagcc aggtatatgc accacttgcc ggtatcagag gttatgtagg cattcgctgg
    20221 agtattctgt aatgtagtgg gtaggtaagt tgttcctgct gctaaaaggc acaaattgca
    20281 aacaggtgat ttgtgccttt tttgcttttt gactggatca tgaagtgctt ggatcgacag
    20341 gtattctggc taaatattac atcatccgtg acgtgacttt cttgtgtagg atgggtaaag
    20401 atgccttctc gttaatgctc ttcatcccca tcagatcatt attaactagc agaaggtaat
    20461 tttgtgaggg attagaatct attattttat tgcccgaact taaagacata cgcagtctca
    20521 agccttgcat aattcttatg gtctcttttg ttttttaagg taaattgcag ttctaatttg
    20581 tgcctccggt tgattgaata aataccgccg agtgcatttt tctgggtatt gaaagataag
    20641 ccacgttcaa tcaactgata gttttcagta aaaataaaca gattgacctt tggattaagc
    20701 agaaagttta gttcgagctt attcctcagg taattgtctg tatcgacgcc gatttttatt
    20761 ttttgttgaa aggtaggccg atacaccaaa atccattttc ctgatttcca tggatgtgca
    20821 aattctatgg aatatcgcag gtcgtgataa tcgaccccag aacctgtacc ataacgatag
    20881 tccaaccctg ctacagccca tggagcaaac tcgtattcca actctccact aaataaggat
    20941 ttttcgaatc gtgaaagatt gtctatcaaa tacaaataat aagtagcttc cacttccagc
    21001 ttcttattga cctgataggc tatcgagaca gaaggccgtg cttcccagtc atcaataaac
    21061 tgggcattgg atgaaaaaaa ggaaagtaag cccaacagga caagagcgat atttctacgc
    21121 actcaattcc tttttttcgg gaatcaagag cgatgccata atacctccgg tcagggaagc
    21181 tacaataact cccagggaga cccattccgg gaattccaca tgatgtgaca ggatcatctt
    21241 tacacctacg aaggcaagaa taacaaccag actatagttg atatatctga atttattcaa
    21301 catgccggat atcaggaaat acatggatcg aaggcccata acggccaaaa tattcgagct
    21361 gaaaacgatg aaaggatctg cggaaatggc tagaattgca ggaatgctgt cgatagcaaa
    21421 aaacaaatcg gtgagttcga tgatgaccaa tgctacgaaa agcggtgtcg cggcttttat
    21481 ccccctcttt ttaataaaaa atttgtcccc gtccatcatg taggttaccg ggtatatttt
    21541 tttaatcaac gagaataatt ttgacttttt aggattgaaa tcctcgcttt tatgtgtaag
    21601 catcctgtat gccgtgatga tcaggaaggc tccaaacaca taaataatcc agctgaaatg
    21661 ggtaatcagg gcaaccccga aaaagatcat gatggcccgg aaaactattg cgcccaaaac
    21721 accgtagaac aaaacttcgt gctggtattt ttgtggtatt gaaaaggatg agaatatgac
    21781 ggcaatgatg aaaatattgt ctacgctaag cgatatttcg atcaggtagc ctgagatata
    21841 cttcaaaact gcattatatg gagttaggct ggtgggattt tcaacccatt gatgttcaaa
    21901 agcgagatat atgattccgg aaaacagcag tgccaaactt atccatacaa gagtccatgc
    21961 agtcgcctcc ttagttttga tgatgtgggg cgtcttgttg aatacaccca ggtccagcgc
    22021 caatgctatt atgataaaag taataaaaat tatccacaac ataacctagc ttattataga
    22081 attcgtattt tttctttttt ggcgccttgt tttttaccgg gaaaaatcat cttgatgatc
    22141 atcggtaaag aggccaggac taaaacggcc aagaccgccc actccaaatt gtcagccacc
    22201 cattcccttt tgccgagtac aaatccaagc gtggttaggc ctccgaccca aaggaatgcg
    22261 ccaagcacat tatagaatgt aaactgtttg aaattcattt ttaccatacc tgcaacgatg
    22321 gggacaaaag tcctggcaat gggcaaaaaa cgcgcaattg caactgcaaa accccctcgt
    22381 ttctcataga attgctgagc ggcatcaata tgtttttgcc tgaggaaaag gaatttcctg
    22441 ccttgataga acagagcatg gccaaatttg cttccaaacc aatatccaac aaagttacca
    22501 agaattgtac ttccgataaa taatgattgc caaaagacga ggtcgatcac tttatggtca
    22561 aaaatatgaa ctgtctggtc ggcacttgcc aatataattc cggaaataaa cagcaggaca
    22621 tcgccgggaa gaaaaaatcc gaagaaaagg cctgtttcta taaaaagaat cacaagaaca
    22681 aggtacagtc cgccctggct catgatccaa tccggatcaa tcaaattggc aaataattca
    22741 aacagtgttt ccacaattta taattttgac aaagtaacag attatttatc aaccggttca
    22801 ctttaattgg ttaatgtttt taaaaacatc aaaacttgat tgatttgttt ttattctatt
    22861 tattaatatc ggttcctttc attcaaaatt tataaagtgt ggttagttga ctgaaataaa
    22921 atgctgatga ctttgaaaat gtaatgacac cagagtgctg tggcatagat aaatagtctc
    22981 ctcggttttt agtggttatt gggatttctt gaaggatgaa gatggctcag gttgcccggg
    23041 ctggttgatc cgactcccgg gaccctgtcc cgtcacatcc ggaaatttag ctgcgaagag
    23101 acgctataaa aactaaatat atctacttgc acggaattga ttgaattact ttaagtgcac
    23161 atctttttac tacctttgtt ctttcaatat atgtgaatta tttaatactt ctattgatta
    23221 tatgagaaac ttacctgtct ttctttcact catttgcctt ttttctatta acaatctttc
    23281 cggtcagagc cagatattta aagaactgca acaaagagag aaggcaggga ctattgtcca
    23341 aaagaagatt gtcaaaaacc tgctttctga ggaaggtgca gaacaagtag gattaagtaa
    23401 ggacggtaac gccatttact tcaatataga tcagcctgta atcgctgaaa taatcgtaca
    23461 tcggcctgcc ctcctaaggg taaagatacc atcggtttca tcgggggagg aactcaccct
    23521 gctcttgttc aacaaaaagc ctttcgctga aggtgctgtc gttactacga gtgccggaga
    23581 cagacatccc atgtccggag gagcttatta ccgtggaaca gttgaaggcg atgagcacag
    23641 tgtggtggca ttaagttttg tggatgggga gatggtgggc gtcgtttcag tcaataatcg
    23701 ccagtttgat atcggcaagg ttgaacattc agatgtacat gtcatctatg atgccactaa
    23761 ggtcaacagg aaggcgccca ccggttgtga ggtaaaatat gtcgagggat acaagcccga
    23821 catgattgga gaggctcaga ttgaaaccag ggctgccgac aagtgtgtca atatttactt
    23881 tgaggtatcg tattctattt atcagaataa aggcagtgtc agtgctgttg agaactatgt
    23941 caatggcttc ttcaacgtcg tctcgacctt atatggtaat gaaaatatcc aggttgtgat
    24001 atccgaaatt ttcgtctgga catcatcgga tccatttccc actaatgatg ctggtgatgc
    24061 cctggattat tttaccggtt acagaacatc cttcaatggt gacattgctc agttactggc
    24121 atataccgat aatggcaatg gagggattgc ctggatagat gtattatgca attttggtgg
    24181 agcgtattcg tacagttatt cggatattga catgacctat aatatgttgc cgaattactc
    24241 ctggactacc aatgtggtga ctcatgagct agggcacaac ctgggttccg aacatacaca
    24301 ttggtgtggt tggcaaggtg gggcaatcga caactgttat actactgaag gtggttgtcc
    24361 tcccggtcca cctccgtcca atggtggcac attgatgagc tattgtcatt tgacaggata
    24421 tggg
//
LOCUS       DAOQQN010000004        22326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_693_length_22326_cov_5.831799, whole genome shotgun sequence.
ACCESSION   DAOQQN010000004 DAOQQN010000000
VERSION     DAOQQN010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 22326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22326
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_693_length_22326_cov_5.831799"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PLG24_00325"
     CDS             <1..612
                     /locus_tag="PLG24_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ23352.1"
                     /translation="ADVIHEFCQKMGIDKVVLTGHSMGGQIAISAALKYPDLVEKLVL
                     VAPAGFETFNKGQRQWFRDVMTVDGVRLTTIEQIRTNYAYNFYNMPDDAQFMVDDRIA
                     IRKASDFNEYCYHVTQGVKAMVDFPVFDNLKNIRQPVLVFFGENDNLIPNRFLNGGKT
                     EKYAKMGNSQLKNSRLVMVPHAGHFVQFEKPDVINEEMKSFLK"
     gene            complement(858..3263)
                     /gene="lon"
                     /locus_tag="PLG24_00330"
     CDS             complement(858..3263)
                     /gene="lon"
                     /locus_tag="PLG24_00330"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HMZ23353.1"
                     /translation="MFDLLHYASGIIEEADNPFIQIDLKDLDKKIDYPELLPVLPIKN
                     TVLFPGVVIPITVNRFKSIRAVEDAYTSDKMIMVLTQIKQDVENPGPDDMYTVGVIAE
                     IHKLIRMPDGSITIVIQGRSRFAVDSFEQSEPYLKGRFTILEYIQPSDTREFEAQISS
                     IKDMARNIIEISPNIPNEANNMLRNIESNTFLLNFIASNLSVDTSGKQKLLETAYLPD
                     FANMLLSNMHDEFNLLQLKGQIESKVRTDLEKQQRDYFLSQQLKTIQEELGQNTFEEE
                     HRNIEEKAAKKKWSAEVKAIFDKEFNKLKRMNPQMAEYGVQINYIETLLDLPWGEYSK
                     DNFDLKKVKKVLDDDHYGLEKVKERILEHLSVLKLKGDMKAPILCLVGPPGVGKTSLG
                     KSIAHALKRKFVRMSLGGLHDESEIRGHRKTYIGAMPGRIIQGIRKAGSSNPVFILDE
                     IDKVGKDFRGDPSSALLEVLDPEQNNSFADNFLEVEYDLSKVLFIATANSLSTIQPAL
                     LDRMEIIDLSGYSLEEKTRIASRHLIPELMANHGLKPRSIEFAEDAVVTIISEYTRES
                     GVRALAQKLSSVMRHYALKIAMGDKFLKKIRVQDIHTILGAQRYSNDIYTEKQPAGVA
                     IGLAWTSVGGDILFIESSLSKGKGKLILTGNLGDVMKESASTALSFVRAHADQIGINP
                     EDFEQYDLHIHVPEGAIPKDGPSAGVTMLSAICSAFAKKPIRPYLAMTGEITLRGKVL
                     PVGGIKEKVLAAKRAGLKEILMCRENEKNVAEIPAEYIEGITFKYVDRMSEVLEYALS
                     ISM"
     gene            complement(3949..4668)
                     /locus_tag="PLG24_00335"
     CDS             complement(3949..4668)
                     /locus_tag="PLG24_00335"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMZ23354.1"
                     /translation="MMKFSGAVIWGLIFAVLVVVGCKKTDNQEVATGLKDTIIVEPTI
                     PPIDSNTDREVDMATVSGNFIIEAPSTINWTASDPVSTHVGTVDVTSGNLEIKDGKIQ
                     SGSFEIDLHTINSTDLKPEDGKEKLETQLKSADFFNVEKFPSAKFVIKKSVVIKGNDK
                     TRHHITGDLTIRDVTKSIGFDAYIIVSNQGGLLTATTPSFEINRVDYGMKYKSGVINT
                     AKDKIISDKIGMLINLRAKKQ"
     gene            complement(4772..5659)
                     /locus_tag="PLG24_00340"
     CDS             complement(4772..5659)
                     /locus_tag="PLG24_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HMZ23355.1"
                     /translation="MGLYTIKDIAQLTSIKPHTIRMWEKRYGILQPKRSENNNRYYCQ
                     SEVSLLSKIAYLTQNGHKIGKLAKLSREELARMAEDTIYNKPDEEKEDLLIKAILRFD
                     SDTIGDTIYRSMEAAGAYETFINMLLPIAGRLNLLMMAGRIRQSHIGHFFNVVKKIFI
                     SEAAKYPPNKSCNTIHLVLYPFRSSTQYISMAAIECGLRQHGIRCTELGQNCPVEDLS
                     EVFISLKPDAFVTVLYEDVATNEFYNIINRMNGFIGNTPLYIVSDEDMKEFPRLPLNV
                     RVFPDVSTLVEYARSLKRD"
     gene            5778..6923
                     /gene="bshA"
                     /locus_tag="PLG24_00345"
     CDS             5778..6923
                     /gene="bshA"
                     /locus_tag="PLG24_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HMZ23356.1"
                     /translation="MKIGIVCYPTYGGSGVLATELGIGLADHGYEVHFITYQKPVRLS
                     SFHQNVYFHEVEQVDYPLFEYAPYESALASKMVDVVRYEKLDILHVHYAIPHATVAYL
                     SKQILARHGIRIPIITTLHGTDITLVGINKTFTPIIEFSIDASDGVTAVSNDLKQQTI
                     ENFDIKHEIRVIHNFINFNRFRKSNKDHFRKAIAPNGEKIITHTSNFRPVKRVGDVVD
                     IFARITKVLPAKLLLIGDGPMRSELEERTRNCPQYENIRFLGKQDAVEEILAISDLFL
                     MPSEKESFGLAALEAMACEVPVISSNAGGIPEVNIDGVTGFLSDIGDVNAMAHNALKL
                     LQNDELLHQFRQNALFQARQFDIDRILPQYIRYYEEIIEKVNAEQVV"
     gene            complement(7010..7249)
                     /locus_tag="PLG24_00350"
     CDS             complement(7010..7249)
                     /locus_tag="PLG24_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23357.1"
                     /translation="MKHIFALIRCVPAGGQAPHLLICTLCVLSGTISILLKTGTAYSA
                     LTSIPTTDELPKHLRVVSVMRKAACHRFYYGNCIL"
     gene            7316..8296
                     /locus_tag="PLG24_00355"
     CDS             7316..8296
                     /locus_tag="PLG24_00355"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HMZ23358.1"
                     /translation="MLCIYHESTDPYFNIATDEYILKYMEEDCFMLWRNDNAIIVGKY
                     QNTLSEINYDYVRENNISVVRRMSGGGAVYHDLGNLNFSFTKSGADSNLTDFEKFTKP
                     IVDVIRSLGVDARFEGKNDLMIDGRKFSGNAAHIYKNKILHHGTILFASEMRNVAEAL
                     RINPVKYQDKAQKSVPKRVTNISEHLREPMTVKEFTEKVMDYVLSIYPDSRLYQFTEE
                     DLAIIRKLRDEKYATHEWNFGKSPDYSFKKAIRTLGGLLEMNIEAKKGVIEDVKIFGD
                     FFSERGIDEIEQALVGVHHEEKAIRGVLSRFDIGLYFSNITVDNLIEAMF"
     gene            complement(8298..8774)
                     /locus_tag="PLG24_00360"
     CDS             complement(8298..8774)
                     /locus_tag="PLG24_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23359.1"
                     /translation="MVFTEKMIERVFDREVDSELEPMVVLNAIAGDQPAIIAYLNQEN
                     LQLLTEEEFDYCTFLTTIIYKVFRENGVEVAEVTPAQIDAAEEANWDILSRATGKKFS
                     QRIDGFFENYVQEDLLAFVEDALEPDELPIVTPEGREPLFITLKTIIDVLNANIKV"
     gene            complement(8860..9669)
                     /locus_tag="PLG24_00365"
     CDS             complement(8860..9669)
                     /locus_tag="PLG24_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23360.1"
                     /translation="MRQYPSKIILFGEHTLLWGSEGLAIPGNQYFGHWAQGNPRHQGT
                     DLYSFADYLSGQKFHNRVDHAGLLEDATNGWFFDSNIPVGYGVGSSGAYSAAIYDRYA
                     CVTDKDASHIRQDLAAIESFFHGSSSGIDPLISYYDRSFHLKDGYPEEVVIADRIRDS
                     FTLTDTGIKRDGSDYIRLFAQKMEDPNFAQKIKELYIPLVTNAIDACLKDDIGRLHEH
                     FFEISYFQLVWMTEFIPFHLIKKWEQQLSDYTGYYKICGAGGGGFLLKIRK"
     gene            10287..11363
                     /locus_tag="PLG24_00370"
     CDS             10287..11363
                     /locus_tag="PLG24_00370"
                     /inference="COORDINATES: protein motif:HMM:NF037527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23361.1"
                     /translation="MKETLERLSEITAPVCVTLILNTHRTTPENQKDIIALKNLISET
                     YERLQREHGTDIAKRYTAKLQGLAEDIDHYHNDHGLMLFVNDEIAEYLRLPIHVTDRV
                     IIDKTFATRSIVRAMKRVSDYYVLVLSKGKARLIEASGDVVVKEVRGEGFPFNDSQFM
                     KLSSAESSNAARASNLAHEFFNRVDKALNKIRNENPLPVVVCAEESNYHLYLAAADHP
                     NIILGHVLLKNIDASPNSIVKDYVAPQVEEMTLLKHRERISELSAAVNSGRALTDLNE
                     IWAAVRVGRGKTIFVEQGYIQAVRKEDGLLKPIALDEVDSKEDINDVVDEMIEHTMKM
                     GGDVVFVRKGDLEQFNKLALATRY"
     gene            complement(11450..12673)
                     /locus_tag="PLG24_00375"
     CDS             complement(11450..12673)
                     /locus_tag="PLG24_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HMZ23362.1"
                     /translation="MKYLIGSLFFVLSFYSCISTKDDINDNKPSVSINENTTKGLQSG
                     AVDIFRQFVRSSYEDDNVIISPLSIQYAFGMAANGASGSTLDEIMRLMGTPSSGLQEV
                     NTGLKDIMQNLKADDKDFTISIANGVFYDPNMFRFNATYEKSLLNLYNAELLQSNFDD
                     KEATVNAVNKWASDHTRERIQKVLDDVQQGEFMFLLNALYMKASWDKGFPPELTVKQN
                     FTTSKNIQNQVDMMYRHEPAILHRNNDEIAVVVPMGNGRLEALFILPNQDGITTYIDK
                     LNRDKIEYFFGKGQVTDMFLKLPKTELLLHYDLKKILLDMGIVLPFSSTADFTKMGTA
                     SGRINLSRAMHDVFMKMDEKGMEGAAVTTIGVENTSVPIAEEVSFDRPFVMVVRDKNS
                     GNYLFMGKIENPAKK"
     gene            12957..13292
                     /locus_tag="PLG24_00380"
     CDS             12957..13292
                     /locus_tag="PLG24_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23363.1"
                     /translation="MKKLLFLFIISLGMISCHKEEIKGDLFTDSCVQSFISKNNLQIV
                     SVDKADCIFYSIYQYEGRFYYEFNCCVCDLIPQIVNCDGSLYLEYDWDKIQAFKKKCT
                     VLPYILVVK"
     gene            13966..15435
                     /locus_tag="PLG24_00385"
     CDS             13966..15435
                     /locus_tag="PLG24_00385"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HMZ23364.1"
                     /translation="MKLLFLFLFNVFCLMLQAKVWHVGPNRDYTRPSQVSSLVDHGDT
                     VYIDPGVYDNDACKWNKNKLKLIGIVGDNGRPVLRYEGYVPNDKGIWVFDNPGSSDDI
                     YIANLIFDGARVPDNKGKNGAGIRFQANNLTVENCLFRNCQTGILEGHGAVKTSNVII
                     RGCEFFDNGYSGYEHHVYINASTDTLVIENCYLHRPRGEGNSIKTRAQNAYILFNFID
                     EEDGNGSYEINIAQGGNNIIMGNVIVQGTSGGNHAIVGYDEALNEQEDFYFINNTVIN
                     RFPGHCRYFNIAPATGIATFRVYNNVFASVPTASHTFISGNVPAVLDSAANYFATDYT
                     QVGFVDPDQGDYSIHGAASGLINQGVAAGTDKHGYPLVPDSMYQGIGKQLLPRIIHGQ
                     AIDLGAYEYATTEKAATIHPLVSVFPNPNTGTFEIRVNPVSKSLPKIYNSAGMKVDAG
                     ISASQEGMIRVCLSGAFPGIYYVVFVSENGSTSVPVMVY"
     gene            16066..16671
                     /locus_tag="PLG24_00390"
     CDS             16066..16671
                     /locus_tag="PLG24_00390"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HMZ23365.1"
                     /translation="MKKCLLTTLFIGAFLYVADAQTQRVDTVFFGGKKMLRVITEKGD
                     TAYLDQLSEVSISSKQQFSSKEEYDRYMRYRRYAAIVYPYALEAMRIYHESREATRSM
                     TKRERKKYMKTLEKELEDQFEEPLKKLTRTQGLILTKMIEKELDEPFYDVIKEIKGGW
                     SAFYYNTTGKFYGYHLRDGYERGKDYILDMVLDDYRLSVKK"
     gene            complement(17080..17811)
                     /locus_tag="PLG24_00395"
     CDS             complement(17080..17811)
                     /locus_tag="PLG24_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HMZ23366.1"
                     /translation="MENIIENIRAIRTRIAEACTRVGRHPDEVRLLLATKTVIPQNIK
                     LAFAAGETLIGENKVQEIKEKYDDLKSITHEMHFIGHLQTNKIKGIIKYNVDCVQSID
                     RFDLADKLNHRLLQTGRAMDVLIQVNTSSEESKFGVAPEEAVDLVYSVSQLSNLRIKG
                     LMTIGLFTNNPEQARPCFRLLSDLRSELNRLNFPGVEMNELSMGMSGDLETAIEEGAT
                     IVRVGTAIFGLRIYPDSYYWNEGKT"
     gene            17999..19048
                     /locus_tag="PLG24_00400"
     CDS             17999..19048
                     /locus_tag="PLG24_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HMZ23367.1"
                     /translation="MSELVLTIDGVEPLELLGENNSKLQLVKARYPDLDISSRGNNIK
                     ISGDKKLTQELKSKIEILVRYLKENKGLPVTIVDTILSGSNPYNDLAIEANSNGGNVI
                     VHNKDGFPIRARTVNQRKLVDATERADIVFAVGPAGTGKTYTAVALAVKALKNRLVKK
                     IILTRPAVEAGESLGFLPGDLKEKIDPYLRPLYDALDDMIPADKLAKFMSDRIIEVAP
                     LAYMRGRTLDNAYIILDEAQNTTSPQLKMFLTRLGPNAKIIVTGDLTQIDLPYSQQSG
                     LKRALSILSDVEGIAQIYLNEEDVVRHRLVKDIIRAYNVSDAEIRAKKEKERTEREAA
                     KEATHSAIDKINNDL"
     gene            19068..19553
                     /locus_tag="PLG24_00405"
     CDS             19068..19553
                     /locus_tag="PLG24_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMZ23368.1"
                     /translation="MSLIILMVSTAILSKAQETKLYNERADATQEIAMAVTIAHKNNQ
                     HVLIQGGGNWCSWCIKFNKFTREDAQIDSMIRADYQVVHLNYSKENKNEAIFAQYGFP
                     QRFGFPVFIILDGAGNRIHTQNSEYLEDGKSGYDRKRVIEFLKAWSPAAIDPKTYQSK
                     K"
     gene            19560..19961
                     /locus_tag="PLG24_00410"
     CDS             19560..19961
                     /locus_tag="PLG24_00410"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HMZ23369.1"
                     /translation="MTSKVIYKGHLETECIHHKSSESIRTDAPTDNNGKGSAFSPTDL
                     CATSLATCMLTIMGIWARDNEVDMDGAYAEVTKHMGTDPRRIVRIDVNLHFPDMNYSS
                     RVQSVFKNIAKTCPVAYSLHPDIEQVVTFNW"
     gene            19977..20645
                     /locus_tag="PLG24_00415"
     CDS             19977..20645
                     /locus_tag="PLG24_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HMZ23370.1"
                     /translation="MATSIQPSTLHFLIDLSINNYRVWFEDNREAYKLARANFLDFIQ
                     EIIDDLAQDDDSVHGLQAKDCAFRIYRDVRFNKDKSPYKVHFGASIKPGGRKSGKAGY
                     YFHLEPGKSFIGGGIYNPESDQLSHIRKKISGDYSGFTKILAEEKFRSLYTDIDRTDK
                     YLLKRVPRGFKATDPAAGYLLLTSYLSTINLDDDLLVSPTLHKYTVGAFQALHPVIRF
                     LNEV"
     gene            complement(20765..21661)
                     /locus_tag="PLG24_00420"
     CDS             complement(20765..21661)
                     /locus_tag="PLG24_00420"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HMZ23371.1"
                     /translation="MNDFHLNQLYKVLTHIDNNLNSDLSLSTIATVGNYSVFHFHRLF
                     KAYTGETLNEYITRKRLENIAAILIRDKTISITQLAYLNGFSSNASLTKTFKKMYNIN
                     PSQFKKKSSSQYDKIIKSKNGQTFEKFDQYLWRINNLINWLHMNATVNVMDLTPIKLV
                     FVSHIGIDGLDDAFSRIFEWASLKELTNGDNVEFVRIYNDSFKITSPNKVRMEIGIPV
                     HQKVTPDSGIQFKEFEPRRCIVGNFEINLPDFEKAWSSLFIWMNENGYKPGKQRPFEI
                     IKNNFNKHPQKKCIVDLYIPVE"
BASE COUNT         6391 a         4621 c         5002 g         6312 t
ORIGIN      
        1 gccgatgtga tccacgagtt ttgtcaaaag atgggtatcg acaaggtagt actgaccgga
       61 cattcgatgg gagggcagat tgctatctct gcggcactga agtatccgga tttggtagaa
      121 aagctggtgc tcgtagcccc tgccggtttt gagactttca acaagggtca gcggcaatgg
      181 ttccgagatg tcatgaccgt tgatggtgtc aggcttacaa cgattgaaca aatcagaacc
      241 aattatgcct acaattttta caatatgccg gatgatgcgc agtttatggt cgatgacagg
      301 atagccatcc gaaaggcttc cgactttaat gaatactgtt atcacgtcac acagggcgtc
      361 aaggcgatgg tggactttcc ggtctttgac aacctaaaga acatacgaca gccggttctg
      421 gtattttttg gagagaatga caatcttatc ccaaaccgat ttctcaatgg ggggaagacg
      481 gaaaaatatg caaaaatggg caatagccag ctaaagaaca gccgtctggt tatggtgccg
      541 catgccggtc acttcgtgca gtttgaaaaa ccggatgtga taaatgagga aatgaagagt
      601 tttctaaaat aaaggcagaa gttgagtata gattaaagga ggaggagcac ttaatgttcc
      661 tcctttgctt tttaaccacc ctccttgatc gggaagaata ttcgaaacga tttgagtttt
      721 ataaaaacgt ccaatcgcta taccggtttc gtcgcgaaca aggattggtt ttatatccgg
      781 gcaatgctat cagatctcat tgtgcccagt ggaaatgaaa agtggccgga taagaataat
      841 ggaataaaaa cacattatta catagaaata ctcagtgcat attccagtac ttcagacatt
      901 cgatcaacgt atttaaatgt aatgccctcg atatattctg ccggaatttc tgccacgttc
      961 ttttcattct cccgacacat gaggatttcc ttcaggcctg cccttttggc agcaagcact
     1021 ttttccttga ttccccccac aggcaatacc ttgccgcgca aagtaatctc tccggtcata
     1081 gccagatagg gtctgatggg tttcttggca aaggcactgc agatagctga taacatagtc
     1141 actcctgcgg aggggccgtc ttttgggatc gcaccttccg gaacatggat atgtaggtca
     1201 tattgttcaa aatcctcagg atttataccg atttggtcgg cgtgtgcacg gacaaagctg
     1261 agtgcggtgc ttgccgattc cttcatgaca tcaccgaggt ttccggtcag gatgagtttg
     1321 cccttgccct tgctcagact ggattcaatg aagagaatat ctccccccac agaggtccag
     1381 gcaagcccga tcgcaactcc tgccggttgt ttttcggtgt atatatcatt gctgtagcgt
     1441 tgggcaccca ggattgtatg aatgtcctgt accctgattt tctttaggaa tttatcgccc
     1501 atggcgatct tcagggcata atgacgcatc acgctactga gcttctgggc aagagcccga
     1561 accccggact cacgggtata ttcactgatg atggtcacga cagcatcttc ggcaaattca
     1621 attgacctgg gctttaggcc atgattggcc atcagttcag gaatcaaatg tcgtgaggca
     1681 atccgagtct tctcttccag gctataaccg ctcaggtcga taatttccat cctgtccagc
     1741 aaggcaggct gaatggttga caagctgttt gctgtcgcga tgaataatac tttggacaaa
     1801 tcgtattcta cttcgaggaa gttatcggca aaggaattgt tttgttccgg atcgagcact
     1861 tcaagcaagg cagaagaggg gtctcccctg aaatcttttc ccaccttgtc tatttcgtcc
     1921 aggataaata ccggattgga cgagcccgcc ttgcgtattc cctgaatgat ccttcccggc
     1981 atagctccga tgtaggtctt tctgtgacct ctgatttcgg attcgtcatg aagaccacca
     2041 agagacatcc tgacaaattt gcgcttcaat gcatgggcga tggattttcc gagtgatgtc
     2101 tttccgactc ccggagggcc taccagacaa agaatgggtg ctttcatgtc gccctttaac
     2161 ttcaggacag aaagatgttc gaggatacgc tccttgactt tttccaaacc gtaatgatcg
     2221 tcatccaaca ccttcttaac cttttttaga tcgaagttgt ccttagaata ttcaccccaa
     2281 ggcaaatcca gcagggtttc tatatagttg atctggacgc cgtattccgc catttgtgga
     2341 ttcatccgct tgagcttatt gaattcctta tcaaagatgg ctttgacttc tgcagaccat
     2401 ttcttttttg ccgctttttc ttcgatgttt ctgtgctctt cctcaaaagt attctgcccc
     2461 agttcttcct gaatggtctt caactgctga gacaggaaat agtcacgttg ttgcttttca
     2521 aggtcggtac gcacttttga ttcaatctgg cctttgagtt gaagtagatt gaactcgtca
     2581 tgcatgttag acaatagcat gttggcaaag tccggcaaat aggcagtttc aagcaacttt
     2641 tgttttccgg aagtgtcgac tgacagatta gaggcaatga aattgagcag gaaggtattg
     2701 ctctcgatgt tgcgaagcat gttattggct tcattcggga tattgggtga gatttcgatg
     2761 atgttcctcg ccatatcctt gatcgatgat atctgcgctt caaactcacg cgtatctgac
     2821 ggctggatgt attccaatat cgtaaaccga cctttcaggt agggctcaga ctgctcgaaa
     2881 gagtccaccg caaatcggga ccttccctga atgacgattg tgattgaacc gtcaggcata
     2941 cggatcaatt tgtgtatttc tgctatcaca cccaccgtat acatatcatc cgggccggga
     3001 ttttcgacat cctgcttgat ctgcgtcaat accataatca tcttatcaga ggtgtaagca
     3061 tcctctacag cgcggataga tttaaacctg ttgaccgtga tgggaattac cactcccgga
     3121 aacagaaccg tatttttaat tggcagtaca ggcagcagtt ccggatagtc gatctttttg
     3181 tccagatcct tcaaatctat ttgtatgaaa ggattgtccg cttcttcaat aattccggag
     3241 gcataatgca ataaatcgaa catgtaatta tttttaaggc tgaatcagcc gattattcaa
     3301 aactatttgg gctttttaag tattgcaaaa gccattccat taaggattgt gcgacaaaaa
     3361 ggcagcccgc cccctcaagg tattgcctgg tgcctgattt caaggagccg gatcttttgt
     3421 aagggccgga tctttcagga atattgtcgg tcgagcgccg agataaatgt gtgttgaccg
     3481 aagaaaccag cgaatacgac gcgattttct tctaaatgat tgtgcttatc aaccgggagc
     3541 gtcccaaaag gacaaaatgg cagttgtatt aattgtctaa gcacaacgag ggtcaggagt
     3601 tatgaagatg tctcatggac aacttcacat gctttcgccg ggtggataac agccgggtgt
     3661 gtatccatca gataagtcat tgtgcaacaa ggggatgatc gcccgacagt ccaattattc
     3721 tcgtgtttgc cttcttcgcg aatagaaaaa aaataaatat tgtggaaggg tatttgcccg
     3781 ttatgtcgag ccagattgta gaggaggcat caatattgca aaacagcttt gctcaaaaaa
     3841 tagaattatc cctaattatc gctgctttag tcaagttgaa gttgattcaa caatctcata
     3901 tatcaagaat ttacttcgcc tataagggaa accagagccg gttgctattt actgtttctt
     3961 tgcccggagg ttgatcagca tgcctatctt gtctgaaata atcttgtctt ttgcagtatt
     4021 gatgacaccg cttttgtatt tcatgccgta atccacccgg ttaatttcga atgaaggggt
     4081 agtggcggtc aacaaaccac cttggtttga cacgatgata taggcatcaa aaccaatcga
     4141 cttggtaaca tcacgtattg taaggtcacc ggtgatatga tgtcttgtct tatcattacc
     4201 cttgatgacg actgattttt ttatcacgaa cttagctgaa gggaattttt ccacattgaa
     4261 aaaatcggcg gacttcaact gtgtttcaag tttttccttg ccgtcttccg gtttcaaatc
     4321 agtagaatta atagtatgaa gatctatttc aaaggagccg gattgaatct tgccatcttt
     4381 tatttccagg tttccgcttg tcacatcgac ggtacctaca tgtgtagaga ccgggtcgct
     4441 ggcagtccag ttgatggtgc tgggagcttc aatgataaaa tttccggata cggtcgccat
     4501 atccacttct cggtcggtgt tgctatcgat ggggggaatg gttggctcga ctataattgt
     4561 atcttttagt ccggttgcaa cctcctgatt gtctgttttt ttgcatccta ctactacaag
     4621 tacagcgaaa atcaatcccc aaattacagc gccactaaat ttcatcattc attctattta
     4681 gaaaattcaa taaaaggtcg atttgacaaa gtaaagttat attaatttgc caatttggga
     4741 gaaaactaaa tgtcaaaata ttttaccccg gtcaatccct ctttagtgat ctggcgtatt
     4801 ccaccagcgt gcttacatcg gggaagactc tcacatttag cgggagtcgc gggaattcct
     4861 tcatatcctc atcggaaact atgtacaggg gtgtattgcc aatgaatcca ttcatcctgt
     4921 tgatgatgtt gtaaaattca ttggttgcga catcttcgta caacactgta acaaaggcat
     4981 ccggcttgag ggaaatgaaa acttccgaca gatcctctac cggacaattc tgacccagct
     5041 ccgtgcacct gattccatgc tggcgcagac cacactcgat ggctgccata ctgatgtatt
     5101 gtgtactgct cctgaacggg taaagaacca ggtgaattgt attacaactc ttgttgggcg
     5161 gatattttgc ggcttcgctg atgaaaatct tttttaccac attgaagaaa tggccgatat
     5221 gtgactgcct tatcctgcct gccatcatca gaaggttgag ccttccggca attggcaaca
     5281 gcatgttgat gaaggtttcg taagctcctg ctgcttccat cgaacgatat atcgtgtcgc
     5341 caatggtatc tgaatcaaac cggagtattg ccttgatgag caggtcttcc ttttcctcat
     5401 caggcttgtt gtagattgtg tcctcagcca tcctagccaa ttcttcccgg gacagttttg
     5461 ccaacttacc tatcttatgt ccgttttgag tcaggtaagc aattttagaa agcaatgata
     5521 cttcggattg gcaataatat ctgttgttat tttccgacct tttgggctgc aggataccat
     5581 acctcttctc ccacatcctg atagtgtgtg gcttgatgga tgtgagttgt gcaatatcct
     5641 tgattgtata tagtcccaaa atttatctcc tctttttgca ttaaaacaat ccattaaatt
     5701 aaattgttta tagccgtaaa gtaggactca tctgtaaaaa gcggtgtgct tccacttcaa
     5761 cttttcaaat acagaatatg aagataggta tcgtatgtta tcccacttat ggcgggagtg
     5821 gcgttttggc gactgaattg ggcattggac tggccgatca tggctatgag gttcatttca
     5881 ttacctatca aaaacctgtc aggctcagtt cttttcatca gaatgtctat tttcatgagg
     5941 tagaacaggt ggattatccg ttgtttgagt atgcacccta tgagtctgcc cttgcttcca
     6001 agatggtcga tgtggtgcgt tatgaaaagc tggatattct tcatgttcat tatgctatac
     6061 cccatgctac agttgcatat ctttcgaagc agatactggc acgccatggt attcgcattc
     6121 ccatcatcac cacgctgcat ggtactgata tcaccctggt cggtatcaat aagactttta
     6181 cccctatcat agaattcagc attgatgcaa gtgacggcgt caccgcggta tccaacgacc
     6241 ttaagcagca aaccattgag aacttcgaca ttaagcatga gattcgcgtt atccataatt
     6301 ttatcaactt caatcgcttt cgcaaaagca acaaggatca tttcagaaaa gcaattgcac
     6361 caaacggaga gaagattatc acccatactt ccaattttag accggtaaaa cgcgttggcg
     6421 atgtagtgga tatttttgcc cgcattacga aggtattgcc cgctaagctg ttgcttatcg
     6481 gggatggccc aatgcgtagt gaactggaag aacgtacacg aaattgcccg caatatgaaa
     6541 atatccgctt tctgggtaag caggatgccg tggaggaaat tttagcaatc agtgatcttt
     6601 tcctgatgcc atccgaaaag gaaagtttcg gcctcgctgc cctggaagct atggcctgcg
     6661 aagtaccggt catcagttca aatgccgggg gtatccccga agtgaatatt gatggagtga
     6721 ccggctttct cagcgatatt ggagatgtga atgccatggc ccataatgcg ttgaagttgc
     6781 ttcaaaatga tgaactgctc caccaattca ggcagaacgc cctttttcaa gcccgacaat
     6841 ttgatatcga ccgcatatta cctcaatata tccggtatta tgaagaaatc atagaaaaag
     6901 tcaacgccga gcaggttgtc tgatcaaata ttttgtgagg aagctgcccg ttgttcaaat
     6961 ttttttcttt aatgatgtaa agtatagtcc attccaggta tcccgctttc tataatatac
     7021 agttgccgta gtagaaccga tgacatgcag cttttctcat cacagacact accctaaggt
     7081 gttttggcaa ttcatccgta gtcggtatgc tcgttaatgc cgaatatgct gtgcctgtct
     7141 tcaaaagtat tgatattgtc cccgataata cgcacagggt acagatcaga agatgtggtg
     7201 cctggcctcc ggcagggaca caccgaatca aggcaaatat gtgtttcacg gaatcaatta
     7261 gcatatttat cgctcttttt ttacttaatt ttgcgttgca aaaaattaat tcgacatgct
     7321 gtgtatatat catgaatcaa ccgacccgta cttcaacatc gccaccgatg aatacatcct
     7381 aaaatatatg gaggaggatt gcttcatgct gtggcgtaat gacaatgcaa tcatagtggg
     7441 aaaataccaa aacacgctgt cggagatcaa ttatgactat gtcagggaaa acaatatctc
     7501 tgtggtaaga aggatgtctg gtggcggagc agtgtatcac gacctgggca acctgaattt
     7561 ttcttttacc aaaagcggag cggattctaa tcttaccgat tttgagaaat tcaccaaacc
     7621 tatcgtggat gtcatccgtt cattgggcgt ggatgctcgt tttgagggaa agaatgacct
     7681 gatgatcgat ggcaggaagt tttccggtaa tgctgcacat atatacaaga acaaaatcct
     7741 tcaccatggc accatcctgt tcgcttcaga aatgcgtaac gttgcagaag ccctgagaat
     7801 caacccggtg aaatatcagg acaaggccca gaaatcagtg cccaaacggg tgaccaatat
     7861 ctctgagcac ctcagggagc cgatgacggt caaggaattt accgaaaaag tgatggacta
     7921 tgtactttct atatatccgg atagccgatt gtatcagttt accgaggagg acctggcgat
     7981 catccgcaag ctcagagacg aaaaatatgc gacacatgag tggaattttg gcaagtctcc
     8041 ggattacagt tttaaaaaag cgatcagaac attaggtggc ttgctggaga tgaatatcga
     8101 agcaaaaaaa ggtgtcatag aagatgtaaa gatctttggg gactttttct ctgagcgcgg
     8161 tatcgatgag atcgagcagg cccttgtcgg agtccatcat gaggaaaagg ccattcgcgg
     8221 ggtgctcagc cgctttgaca tcggcctata tttttccaac atcacggttg acaatctgat
     8281 agaggccatg ttttgaccta tacttttata ttggcattga ggacgtcgat gatggttttg
     8341 agcgtaataa ataatggttc acgaccttcg ggggtcacga tcggcagctc gtccggctct
     8401 aaagcatctt cgacaaaggc gagcaggtct tcctgcacat aattttcaaa aaatccgtcg
     8461 atacgctggc tgaatttctt gccggtggca cgggataaga tatcccagtt ggcctcttcg
     8521 gcggcatcaa tttgtgcagg agttacttcg gccacctcca ctccgttctc cctgaacacc
     8581 ttgtaaatga tggtggtgag gaaggtacag taatcaaatt cttcttcggt cagtagctgt
     8641 agattttcct gattgagata ggcgatgatg gccggctgat ccccggcaat ggcgttgagg
     8701 accaccatcg gttccaattc agaatccact tcccggtcaa aaaccctttc aatcattttt
     8761 tcagtaaaga ccatatcgtt aagtttaatt tattcttgct ttgaatgaaa ttgataccca
     8821 tcttgcaagc gtccggcatt cacagaatga gttcttgtac tattttctta ttttaagcag
     8881 gaatccacca cctccggcac cgcatatttt gtaatatccg gtatagtcgg acaattgctg
     8941 ttcccatttt ttaatcaaat ggaagggaat gaattctgtc atccacacca actgaaaata
     9001 acttatctca aagaaatgtt catgaagtct ccctatatcg tccttcaggc aggcatcgat
     9061 ggcattggtc accaagggaa tgtacaattc tttgatcttt tgggcaaaat taggatcttc
     9121 cattttttgg gcaaacaatc taatatagtc ggagccatcc cttttgatgc ctgtatccgt
     9181 cagggtgaag gagtccctaa tccgatccgc aataacaacc tcttccgggt agccgtcttt
     9241 cagatggaaa gatcggtcat aatatgaaat cagggggtca atacccgaac tggaaccatg
     9301 aaaaaaggat tcgatggcag caaggtcttg ccgaatgtgt gaagcgtcct tgtcggtcac
     9361 acaggcatac ctgtcgtaaa tggcggcact atatgccccg gaagagccga caccatagcc
     9421 caccggaata ttggagtcaa aaaaccaccc attagtggca tcttcgagca atccggcatg
     9481 atcaacccgg ttatgaaact tttgtccgga taaataatct gcaaagctgt acaagtcggt
     9541 accctgatgt cttggattgc cttgtgccca gtgcccgaaa tactgattgc ccggaatggc
     9601 caagccctca ctgccccata acagggtatg ttcaccgaaa agtatgatct tggagggata
     9661 ttgccgcatc acgtcctctg tttaccggaa gctaaggtaa atgtagcgca ggaaataatg
     9721 aaataaaatt acgtttggaa gaaacaccct tgaaatcagg gcggaatcca tcagacagga
     9781 tcgtattaat caagggtcgt cattagctca aaaaatctat ctgtgccatt tcgggtaagg
     9841 aaaatggtaa aaaccaaata agattaaagt ctgacaaaca cgcgtatttc cgaattgaaa
     9901 attgctaaaa tttaattgtc ataattaagt tttaaaacgg tctgtttttt tgcaataaaa
     9961 tatttttcga aattgcataa acaactgata tataggatgt tttgtattaa atacggaact
    10021 tttgacctgt taatttcgtt gaaattatag cgtatatttg atatgaaacc gaaagcaaat
    10081 atttcaatca tcaggtctgt gaaggaattg tcctgatgag caaataaggg tagtaataaa
    10141 ttccagcaaa gcataattac atagggcaat aaccagattg ggtgagtccg gaaagagagg
    10201 taggtccctt ctacctaatg atattggtat gtgtaattgg caaaagaagg aaaaaatgaa
    10261 ttgtaaatag tcaaaaattg aaaagcatga aagaaacttt ggaaagattg agcgaaatta
    10321 ccgctccggt atgtgtgacc ttgattttga atactcatcg aaccactcct gaaaatcaaa
    10381 aagacatcat cgccctcaaa aatctcatct ccgagaccta tgaacgattg cagcgtgagc
    10441 atggaacaga tattgccaag cggtacacgg ccaaactgca gggactggct gaagatattg
    10501 accactatca caatgatcac gggttgatgc tgtttgtcaa tgatgagatt gctgaatatc
    10561 tgagattgcc gattcatgtg acagacaggg tgattatcga caaaacattt gctacaaggt
    10621 cgattgtgcg ggcaatgaag cgagtatccg actactatgt actggtactt agcaagggta
    10681 aagccaggtt gattgaagcg agtggcgacg tagtagtaaa agaggtcaga ggagaaggat
    10741 tcccgttcaa tgacagccag ttcatgaaat taagcagtgc agaaagctcc aatgctgcca
    10801 gggcttctaa cctggctcac gaattcttca atcgtgtgga caaggccctc aacaagataa
    10861 gaaacgaaaa tccactgccg gtggttgtat gtgctgagga atccaattat cacctttatt
    10921 tggcggcggc ggatcatcca aacatcatac tgggacatgt attgctcaag aatattgatg
    10981 caagtccgaa cagtattgta aaggactatg tggctccgca ggtggaagaa atgacactct
    11041 taaagcatag agagcgcatc agtgaattgt cggcagccgt caacagcggt cgggcattga
    11101 ctgatctcaa cgaaatctgg gcagcggtaa gggtcggacg cggaaaaacc atttttgtgg
    11161 aacagggata cattcaggca gtgcgtaagg aggatggtct gttgaagcca atcgcattgg
    11221 atgaggtgga cagtaaggaa gacattaatg atgtcgtgga tgaaatgata gaacatacga
    11281 tgaagatggg aggcgatgtg gttttcgtaa gaaagggtga cctcgaacaa ttcaataaac
    11341 tcgctttggc aaccagatac tgatctgcaa tttcagagga tagtttggat aaaaaaagag
    11401 aggcagaagc ctctcttttt gtttttgtat tataaacaga ttcaacaaat catttcttgg
    11461 ccggattttc aatcttgccc ataaacaaat aatttccgga attcttatcc cggacaacca
    11521 tcacaaacgg cctgtcaaaa ctcacctcct ctgcaatggg cacggaggtg ttctctacac
    11581 caatggtggt gaccgctgct ccttccattc ctttttcatc cattttcatg aaaacatcgt
    11641 gcatcgccct ggaaagattg atcctgccgg aggctgttcc catctttgta aaatcggctg
    11701 tggaagagaa tggaagcacg atgcccatgt ctaacaaaat cttcttcaga tcataatgta
    11761 acagtaattc tgtcttaggg agcttgagaa acatatccgt cacttggcct ttcccaaaaa
    11821 agtactcaat tttatcccgg ttcaacttat cgatataggt tgttatcccg tcctgattgg
    11881 ggaggataaa caaagcctca agccgaccat tgcccatggg aaccaccact gctatctcat
    11941 cgttgtttcg atgcaggatg gcaggctcat ggcgatacat catatctacc tggttttgaa
    12001 tattttttga tgtcgtgaaa ttttgtttta ccgttaattc aggcgggaat cccttgtccc
    12061 agcttgcctt catatacagt gcattgagga gaaacatgaa ttcaccctgt tggacatcat
    12121 caagcacttt ctgaatcctt tcccgggtgt ggtcggaagc ccatttgttg acagcgttga
    12181 ccgtggcctc tttatcatcg aagttggact gaagcaattc ggcattatac agattgagca
    12241 aggatttttc atatgtagcg ttaaaccgga acatattcgg atcataaaac acgccattag
    12301 caatcgatat ggtaaaatcc ttgtcatcag ctttcaggtt ttgcatgatg tctttcaagc
    12361 cagtattcac ttcctgtagg ccggaggaag gagtgcccat cagtctcatt atttcgtcta
    12421 gcgttgagcc ggatgcgcca tttgcagcca taccgaaagc atactggata cttagtggag
    12481 aaatgatgac attatcatct tcataagacg agcggacaaa ttgtctgaaa atgtcaacag
    12541 cgccggattg aaggcccttt gtcgtatttt cgttgatgga gacagaaggc ttgttgtcgt
    12601 tgatatcatc cttggtggat atgcaggaat agaaggacaa cacaaaaaaa agtgaaccga
    12661 ttagatattt cataacaaat tgattttcta ttattaacgc aaagaaaagg gaagacgttt
    12721 gccctaggac gattttcatg aaataaatat gatcattcgg gaattttacc gattgattcc
    12781 attgaatgaa gtttctgtta gaaacatttt agttaattca agctgatgac tcattacata
    12841 atacaataag ggatgtcggg caaacaggcc taaagaaaat taaatagaaa caaatatatt
    12901 atcaaccagc taacctaaga gcttttccca tcgttatata tccaaatgga cttcatatga
    12961 aaaagctgct tttccttttc attatttccc tggggatgat atcctgtcac aaggaagaaa
    13021 ttaaaggcga tttatttacg gattcctgtg tacagagttt tatttcgaaa aataatcttc
    13081 agatagtttc agtagataaa gcagactgta tattttattc catttatcaa tatgagggac
    13141 gattctatta tgaattcaat tgttgtgttt gcgacctgat accacagatt gtcaattgtg
    13201 atggaagcct gtatttggag tatgattggg acaagattca agccttcaag aaaaaatgta
    13261 cggtacttcc ctatatcctt gtagtgaaat aaggatttga caaattcacc tgcaacagga
    13321 tgcttttttt aaatatttaa aaagtcgttt ctcaaagcat aaatacccga ctcatacaag
    13381 ggtcattgga taatccctta ttgaggagaa ttctattcgc actgttttca gccatgtaat
    13441 caatcagaag catgggagag aaaaaatatt ttcttaggaa tcacggagat gtgaaagacg
    13501 tcctcagatc ggcttattgt tccgattaca caggattctc taatattttg tgacacagca
    13561 ttttatcatg caatttcaaa ggaagcatat ttttgaatct acttatctaa gtacgctttt
    13621 aaaggaatat ttcaatatgg aatgaagttt caatagtgat aaattccgaa ggataataat
    13681 aaaacaggct tacgatcacg ataattctcc cggttaatag aaatcaagcc cctaaataaa
    13741 aattgttgat tgctgcagat tggtaagata gcaggggttt tctttattaa aataccaaaa
    13801 aggaaaaaga aagattgagt aaaaagttgt ttcgtaacgc agcgattcaa cagacaatag
    13861 tatatttaca ttttgagttg acagtttgtc ataaattgtg tataaataaa ttttaagtcc
    13921 accctaagca attccctgct ttttaatact taaacctcta tcaaaatgaa attattattc
    13981 ttgtttttgt tcaatgtgtt ctgtttaatg cttcaggcca aggtgtggca tgtcggtcct
    14041 aaccgggact acacccgacc aagccaggtg tcatcactgg tggatcatgg cgatacggta
    14101 tatatcgatc ccggagtata tgataatgac gcttgcaaat ggaacaaaaa caagctgaaa
    14161 ctaatcggaa ttgtgggtga taatggcagg cctgtactgc ggtatgaggg ttatgtcccc
    14221 aatgacaagg gtatttgggt gtttgacaat cccggaagtt ctgatgatat ctatatcgcc
    14281 aatctgatat ttgacggagc cagggtgcct gataacaaag gtaaaaacgg tgccggtatc
    14341 cgttttcaag caaataatct gactgttgaa aattgtctgt ttcgcaattg ccagacgggc
    14401 atcctggaag ggcatggagc cgtgaaaacc agcaacgtca tcatacgagg atgcgaattt
    14461 tttgacaacg gctattccgg atatgaacac catgtatata tcaatgcatc gacagacacc
    14521 ctggtcatcg aaaactgcta tcttcaccga cctcgcgggg aaggtaattc tatcaagacc
    14581 agggcacaga atgcgtatat cctgtttaat ttcatagatg aggaagacgg taatggcagc
    14641 tatgaaatca atatcgccca gggcggcaac aacatcatca tgggcaatgt gattgtccag
    14701 ggaacttcag gtggcaacca tgcgatcgtt ggatatgacg aagcactcaa tgagcaggag
    14761 gacttttatt tcatcaacaa cacagtcatc aaccgattcc ccggtcactg caggtatttc
    14821 aatattgcac cggcaacagg aatagcgaca ttcagggtgt acaataatgt atttgcttca
    14881 gtacctacag cgtctcatac gtttatctcc ggtaatgtgc ctgcagtgct ggacagtgct
    14941 gccaactact ttgccacgga ttatactcag gtcggctttg tggatcccga tcagggggat
    15001 tattccatcc acggtgccgc ttccggtctg atcaatcagg gtgtagcagc aggtacagat
    15061 aaacacggct atccgcttgt gccggacagc atgtatcagg gaataggaaa gcagctgtta
    15121 ccgagaatca tacatggtca ggcgatcgat ctgggtgcgt atgaatatgc aactacggaa
    15181 aaagcagcaa cgatccatcc attggtttca gtatttccca atccaaatac aggaacattt
    15241 gagataaggg tgaatcctgt tagtaagagc ctaccaaaga tttacaattc ggcagggatg
    15301 aaagtagatg ccggaatttc tgcctctcag gaaggaatga tcagggtctg cctttccgga
    15361 gcctttccgg gcatttacta tgttgttttc gtatcggaaa atggcagtac gagtgtaccg
    15421 gtcatggtgt attgaaacca agggagataa agtgtatttc ggctaaggca tgggtggtcc
    15481 atatttgttt gccaagtccg ggccttgcgg tttgacgatc ctgcataaag tggtaaggac
    15541 tgccagcaaa aggagtagtt tagaatggca gcggggttga ttagtcctgc ccgcgactga
    15601 attttccatt cggagttaaa tatttatttg tcagggacat atagaatgtc aggaggggga
    15661 caatctaatc agcatcttcc cataggaaaa tttcttccag acatccacaa agattatccc
    15721 gagtgtttca aagaattcgt aagatcctga tttatctgcc attccttacc gtgaatttca
    15781 aatggcaaat tgaggcacaa ccataataat ttaaaaaaca tactaattac ggttgatatt
    15841 ttctaatcat gaaggaggcg ttatttgtaa taaaataaat acatttattg atgcaatgaa
    15901 ttcaccatat taaaatcaag ccggatgaaa attcattaaa aatttacatt ttacacagcc
    15961 aaaataaaaa aaccagttgc gatattgtta ttttttttca ataatttgcc ataaaatcaa
    16021 atcaaatcac gttcttttgc gttcttgtga ataatgtatc aaaccatgaa aaagtgcctt
    16081 ttaacgacgc tatttattgg ggcattccta tatgtcgccg atgcacagac ccaaagagtg
    16141 gacactgtct tttttggagg caagaaaatg cttcgagtca tcaccgaaaa aggagatact
    16201 gcttaccttg accaattgag cgaagtctcc atttcctcta aacaacagtt ttccagcaag
    16261 gaagaatacg accgctatat gcgatatcgt cggtatgctg ccattgtata cccctatgct
    16321 ctggaggcta tgcgtatcta ccatgaatca cgagaggcca cccgtagtat gacgaagcgc
    16381 gagcgcaaga agtatatgaa aacgctggaa aaggaattgg aagatcagtt tgaggagccc
    16441 ctaaagaaac tgacaaggac acaaggcttg atcttgacca agatgataga gaaggagctg
    16501 gatgagcctt tttacgatgt tatcaaagaa ataaaaggtg gctggtcggc attctattac
    16561 aacaccaccg gaaaatttta cggatatcat ctgcgggatg gatatgagcg cggcaaggat
    16621 tatatcttag atatggtgct ggacgactat cgactaagtg taaaaaaata gtgttaagtg
    16681 aaaggtgaaa aagcataagt gctggatgga agagaatgtc ttgtgtgata aaggggcaca
    16741 cagtaaggtg catgaaattc aacatcaaat gcagatttaa cctaagacac aacaatttca
    16801 gatttacttg ggataatagg tattcacaac ttcaaatata ctcgtgaata gaagggtttc
    16861 agtaataatg ctcccgaagc agtaaaaccc agatggacgg tgagcactct ggttagcctt
    16921 tcagattccg gccaggttgt atctgcaagg tttcacagag agtcacactg tgtttctcaa
    16981 agaatttgta ataattcaga ttgaaatatc cgcagagatt caaaatgctg ccaaaaccct
    17041 caacattggc acatgagtag gctcagggag aaagcaccat taggttttcc cttcattcca
    17101 ataataacta tccggataga ttcggagtcc gaagatagcc gtgcccacgc gcacgatggt
    17161 ggcaccttcc tcgatggccg tttccagatc accgctcatt cccattgaca attcattcat
    17221 ttccacgccg ggaaagttaa gcctgttgag ttcacttcgc agatcgctca aaaggcggaa
    17281 gcagggtctg gcctgttctg gattgttggt gaaaagaccg atcgtcatca gcccctttat
    17341 tcgaagatta gagagttgag atacgctgta tacaagatca acggcctctt caggagcaac
    17401 gccgaattta ctttcttctg atgaagtgtt gacctgaatc aggacatcca tagccctccc
    17461 tgtctgtagc aagcgatggt ttagcttatc ggcaaggtcg aagcgatcga tggactgcac
    17521 acaatccacg ttgtatttga tgatgccttt gattttattg gtctgcaggt ggccgatgaa
    17581 gtgcatttca tgagttatgg actttaagtc gtcatatttt tctttgattt cctgaacctt
    17641 attttcgccg attagtgtct cgccggcagc aaaagccaac tttatatttt gtggaatgac
    17701 ggtctttgtg gcaagaagaa gccgcacctc atccgggtgc ctgcccactc gagtacacgc
    17761 ttcagctatt ctggtgcgga tggcccggat attttctatg atattttcca tgatattagc
    17821 ttttttactt cgtaagggct tttaatcacc taaaattttt cttgatatca aaacccggga
    17881 ttttatgctc aggtttattg acaatctcac gaaggctgtt tatttttgtt ctaattaagg
    17941 aactacaatc ccctttattt gttctaaaga aaataaaatc agaaaggtta taaaatcatt
    18001 gagcgaatta gtactaacca ttgatggtgt cgaaccactc gaattacttg gagaaaacaa
    18061 ctccaaacta cagttggtca aggccagata cccggatctg gacatcagtt cacggggtaa
    18121 caatatcaag atttccggag acaagaagtt gacacaggaa cttaagtcaa agattgaaat
    18181 tcttgtccgg tatcttaagg aaaacaaagg attgcccgtc acaattgttg atacgatcct
    18241 ttcgggttcc aatccataca atgatcttgc catagaggca aacagcaatg gcggcaatgt
    18301 catagtccac aataaggacg gatttccgat tcgtgcacgg acagtcaacc aaaggaagct
    18361 ggtagatgcc acagagcggg cggacatcgt ctttgctgtt ggtcctgccg gtaccggaaa
    18421 aacctatact gccgtagctc tggctgtaaa ggctcttaag aacagactgg tcaagaagat
    18481 cattctgaca aggcccgcgg tggaagccgg cgagagttta ggttttctac ctggagacct
    18541 taaggaaaag atagatccgt atcttcgacc attgtatgat gcattagatg atatgatacc
    18601 agcggataaa ttagcgaaat ttatgtcgga caggatcatc gaagtggcgc cactagccta
    18661 catgcgaggc cgaacgctgg ataatgccta catcattctg gatgaggcgc agaacacgac
    18721 ttcgccacaa ctaaagatgt ttctaacacg actcgggcct aatgccaaga tcatcgtcac
    18781 cggtgacctg acgcagatcg atctgccata cagccagcaa tcagggctta agcgggcact
    18841 gtccatactt tcagatgtgg aaggtatcgc ccagatctat ctcaacgaag aggatgtggt
    18901 gcgtcaccga ttggtcaagg atatcatccg tgcctataac gtcagtgatg cagagattcg
    18961 ggcaaaaaag gaaaaggagc gcacggaaag ggaggccgcc aaagaagcta cgcacagcgc
    19021 aattgataaa atcaacaacg acctgtaaaa tgaagggaat acactcaatg tcactcatta
    19081 tccttatggt gtctacagcc atactatcca aggcacagga gaccaagctt tataatgaaa
    19141 gggcggacgc cacccaggaa atcgccatgg ctgttacaat agcacataag aacaaccaac
    19201 atgtccttat ccagggtggt ggcaactggt gttcctggtg tatcaagttc aataaattta
    19261 ccagggagga tgctcagatc gattctatga taagggctga ttaccaggtt gtccatctga
    19321 attacagcaa ggaaaacaag aatgaggcga tatttgctca atacggattt ccgcagcgct
    19381 ttggtttccc ggtatttatc atcctggatg gtgccggcaa tcgcatacac actcagaaca
    19441 gtgaatatct tgaagatggc aagagcggtt atgacagaaa gcgtgtcatt gaatttctca
    19501 aagcatggtc gccagctgct atcgacccta aaacctatca atccaaaaaa taacgcgata
    19561 tgacatcaaa agttatctac aagggacatt tggaaaccga atgcatccac cataagtcct
    19621 cagagagcat ccgcaccgat gcgcccacgg acaacaatgg aaaaggttcg gccttcagcc
    19681 caacggacct gtgcgccaca tctctggcca cgtgcatgct taccatcatg ggtatttggg
    19741 cacgcgacaa tgaggtagat atggacggcg cctatgcgga agtgactaag cacatgggga
    19801 cggatccaag aagaatcgtg cgtatcgacg tcaatttaca cttccctgac atgaattatt
    19861 cttccagggt tcaatctgtg ttcaaaaata tagctaagac ctgtcctgtt gcctatagtc
    19921 tgcatcctga catcgagcag gtcgttacat tcaactggta ggaagatcaa tctgcgatgg
    19981 caacatccat acagccttct acgctccatt tcctgatcga cctaagcatc aacaactacc
    20041 gtgtatggtt tgaggacaac cgtgaggcat ataaactggc gcgggctaat tttctagact
    20101 tcatacagga aataatagac gatctggctc aagatgatga ttcggtccat ggattgcaag
    20161 ccaaggattg cgccttcaga atatatcgtg atgttcgctt caacaaggac aaaagtccgt
    20221 ataaagtaca ttttggggcg agtatcaagc cggggggaag gaaatcgggc aaagcgggtt
    20281 attactttca tcttgaaccg ggcaagagtt ttatcggagg aggtatctat aaccctgaaa
    20341 gtgatcagct ttcccacatc cgaaagaaga tcagcgggga ctattccggg ttcactaaga
    20401 ttctggcaga agaaaaattc cggtcgttgt acactgatat tgatcgcacc gataaatacc
    20461 ttctcaagcg tgttccaagg gggtttaaag ccactgatcc tgccgccgga tacctcctgc
    20521 tgacaagtta cttgagtaca attaatctgg atgacgatct cctcgtatct ccaacgctac
    20581 acaaatatac agttggggcc tttcaggcgc tacatccggt tatccgattt ctgaatgagg
    20641 tgtaagaatg accattagcc acctgtagag accttcgtct tgttgaagtc aaaagggttg
    20701 cattcatggc caccatatac agtatatggg ccgggcacaa caggacatcg aaaacgaagg
    20761 ttgtttattc taccggaata tacaggtcca cgatacattt tttttgtgga tgtttattga
    20821 aattattttt tataatttcg aaaggtcttt gtttcccggg cttataacca ttttcattca
    20881 tccatataaa tagggacgac caagcttttt caaaatccgg cagattgatt tcaaagtttc
    20941 ccacgatgca tcgtcgcggt tcaaattctt tgaattgaat accggaatcc ggagttactt
    21001 tttgatggac agggatacca atttccatac gtactttatt cggagaagta attttaaagc
    21061 tatcattata gatacgaacg aattcgacat tgtcgccatt agtaagttct tttagcgaag
    21121 cccattcaaa aatgcgtgag aaggcatcat ccaaaccgtc tattccgatg tggcttacaa
    21181 aaaccaattt gatgggggtg agatccatca cgtttacagt tgcattcata tgtaaccaat
    21241 ttattaagtt attgatgcgc caaaggtatt gatcgaattt ttcgaaagtt tgtccattct
    21301 tgcttttaat aattttgtca tattgagacg agctcttttt tttgaattgg gaagggttga
    21361 tattgtacat tttcttgaag gtcttagtca aggacgcatt gcttgaaaac ccatttaaat
    21421 aggcgagttg ggtgatggag atggttttgt ctcggatcag gatagctgca atattttcga
    21481 gtcgttttct ggtaatatat tcgtttagtg tttctccggt ataggccttg aaaagcctat
    21541 ggaagtgaaa aacagaataa ttgccaacag ttgcaatggt agaaagggac aggtccgagt
    21601 taaggttatt gtcgatatgg gtcaacactt tatacagttg attcaaatgg aaatcgttca
    21661 tttagaatag attgcgtcga tagttcaagg atgctgaaaa atgctttctt agtttgaaac
    21721 agtattttaa acaaaaataa aaactgtcgt gctgatcaat gggataaaat tggatattga
    21781 acagaagagg agatgaaagg gggctctgaa gtaggtagat gattaatagc caccatgctt
    21841 gacggtattg cctttcgggg cggctttgcc aaaattcaga tgatttacta gggaattaaa
    21901 tttttcattt gcctccaaat ccttgtgcat ggcggttttt gaattattac tttcctacct
    21961 acttcgattt tttgggaggg agagagtgat gtcgcctctg aagtaggtag atgattaata
    22021 gtcacaatgc ttgaaggtac tgcctttcgg ggtagctttg ccaaaattca gatgatttat
    22081 tcaggaatta aattttccat ttgcctccaa atccttgtgt atggcggttt ttgagttatt
    22141 actttcctac ctacttcgat ttttggggag gatgagggtg atgtcgcctc tgaagtaggt
    22201 agatgattaa tagccaccat gcttgacggt attgcctttc ggggcggcct tgccaaaatt
    22261 cagatgattt actcgggaat taaatttttc aattgtcgcc aaatccttgt atatagcggt
    22321 ttttga
//
LOCUS       DAOQQN010000005        21522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_729_length_21522_cov_5.566218, whole genome shotgun sequence.
ACCESSION   DAOQQN010000005 DAOQQN010000000
VERSION     DAOQQN010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 21522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21522
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_729_length_21522_cov_5.566218"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..4972
                     /locus_tag="PLG24_00425"
     CDS             44..4972
                     /locus_tag="PLG24_00425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HMZ23372.1"
                     /translation="MFFVPGELKIEPRLLDKSLKGTVRARVSCYSGFTLPSILQHRLV
                     TLKLRDHFTAERSYFDVHVDHNALILLSEEQSVEYISFAPETFMPINDKKRSFEGIQG
                     VNASAGAGGLGLNGAGVTIGHGDNGSFNHIDLNDRVINFSIYTGGNHSAHTAGTEAGA
                     GLLDPRRRGLASDATVLNDDFFDIITEASSYYLGNNMHVTNNSYATLDAVPNCTQVGD
                     YNIYSQESDNSTITYPDLLHVFAAGNYGTTSCPPNGDYNNLPDGPQCAKNTIAIGALI
                     SGEGNAPANYTSKGPTDDNRLKPEISTIGTATSCINGNAYSGSSGTSMAAPSVTAAVA
                     LMQEAYTGIHGGTFPPAALIKAILCNTSDELGAEGPDFTTGYGKLNINNAVRHTLNGN
                     HVLNAISQGSTQDVAITIPSGTAKLAVTLAWTDPAGNPLILSQLVNDLDLEVIGPSGT
                     TLPWFIAGIGQPVQRAVDNLNNLEQVTILNPVSGNYTLRVKGTSVVGSQQYALTYRTI
                     TPDINIQAPFGEEKYLAGESVYIQWDCPVDPSGSFLLEISTDGGATWSTITNKAATAR
                     NHNFSMPNVNTRQAQVRISATGLTTDVSSDFTVSGVPAGLAATIPCQGYANLTWNAVS
                     NATGYKIYKKVGSVMVFVDNASTNSYQLSGLTPNVQELIGVTATFPGDIESRRSGGIF
                     VNAVGGACPWSNDMEMVSLLTPVSGRKYTSGELTSSQVITIRLKNLGSSSASGFQVYY
                     KVNGGSEIIENYTGSIATNTTADYTFTTTANLAAIGNYSIEAGIWGAPDAPLIANNIL
                     STLVKHVANDPVTLTQDLNTTYKVDFESATSATYSQSYFAMDGIPMFDFVAHSNDSRA
                     RTYITSGLARSGTKALSLDSKTISTMAVNDAIMTVNLSNYSSSGTVLFDFSYLNHDAS
                     LSVNDTVWIRGNDSSPWLPLYALALNQAAPGEYKNVYGLNLTSFLQNAGQALSSSTQI
                     RFGQSGNGQIVSRNALQGYTFDDIVLHLVSTDAALTQIVSPSRKACESGSNALITIRI
                     DNNSQISLSGVKAWYRINNNAAQGPFSIGTIPANGNLQFSFPPVDFSAYTDYTLSVWI
                     NEPSDTYLLNDTIKNYKFHNSPLINTFPHLQTFETDDGGWYAYGQNSSWAWGTPGKTK
                     INKAANGTKAWTTGLTGPYNNLETSYLESPCYDLSGLASDPMISFSMAHEIETNYDKA
                     WLEVTGDGVTWIKLGSLNSGVNWYNKQSYWDNALSYYHVASHTIPRSLLGSATSVSFR
                     FVLVTDQGLVLEGLAIDDIHIFEPKSIYTGNNQTVTVSPLVSGWTDVYSGQNMIASLR
                     PNNSQSLGNTDVAVYFNPSNDQRHDLNQYYLDRNFTIVPTNPLSSGTVTVRLYFTDEE
                     VNKSRNAPSGYDKPTDAYELGVTQISSNSDDGIYGNESNPSVKYFSCLNTQTTPFNTG
                     YYIEFDVTGFSEFYLNPGGINNNQPLPLKILSFEASALTEQHAARLSYRLVDGVNVRS
                     VDIERSTDGRFSNARIIASSVYTEDLKIFIDNVPPTVENVYYRLKITDRDGAVSYSFV
                     RTVHLPDELSVQVSPNPTRNTPVKVLVSGSRFGQSASVELSDINGQTCRAFRFVPNGD
                     PLFMNTEGLTSGVYILTVKVGEGLKQVKVVVL"
     gene            complement(5102..5770)
                     /locus_tag="PLG24_00430"
     CDS             complement(5102..5770)
                     /locus_tag="PLG24_00430"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ23373.1"
                     /translation="MSGERINYDRVAGIYDITSRIVFLGKQQRIQRQVLRFIRSWDRV
                     LIAGGGSGWILEDDYIQSLKGVDIDFVDNSARMLRLASGRRLGPFNVQFRQENIFDFM
                     SSGQYDVIFTSFLFDNFEAEECRALFDRLDGLLMHGGRWLNCDFSINNRYGAWWKKTI
                     LSLMHHFFNCMAGVSTRSLTEDINLFEGGGYVLEYEEYLYLHFLSGKVFSKVKEAKGI
                     QGEP"
     gene            complement(5767..6681)
                     /locus_tag="PLG24_00435"
     CDS             complement(5767..6681)
                     /locus_tag="PLG24_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23374.1"
                     /translation="MDMKIGRLWESVLFGNYLVGTLAVGLCIETAFQVSLPFAAWSDY
                     LIIFFLPIAYYTYAYLKVGKEAPDENPRSRYYVRNAKVLWQWEVGMIGIVMLATFIPL
                     ISGKFYLSNISWPWYLLSGTTFCMALFYYGLISRKYFGFDIRQSHWLKSFIIGWVWGY
                     FSTALPVIFLNARGISIDLDIYLWLWFFIKNWMFCTVNSIMFDIKDYPTDLNARLRTF
                     VVSFGIRRTIYRILFPLLLAGMISFSLFAREMNFSLARYLFNLLPFLATMAIAFSMLR
                     PRSIFFYLVIIDGVLIFKALCGIIGALL"
     gene            complement(6749..8407)
                     /locus_tag="PLG24_00440"
     CDS             complement(6749..8407)
                     /locus_tag="PLG24_00440"
                     /inference="COORDINATES: protein motif:HMM:NF025650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusF/SusE family outer membrane protein"
                     /protein_id="HMZ23375.1"
                     /translation="MKKIYNLFVLTACLSFFFTACKKFDDLETYSNGKAVTITASSLQ
                     VAPPASDSDNVVLTLNWSDPQYSTEKANYKFLIEIAEKGQNFANASQSVVIGEFKKNF
                     TGRELNNILAGFGFLYDEPHDMEVRVASSYRNNNERYYSNILTINMTAYKGPSTSIDA
                     TTMNIIGEAAKGWDVDVPMNLIGTKKFAVVTELQWGKEFKIRRDAGSWDINWGLADDE
                     TFEFGKGMGIKLNGPNFKLPDGSGSDLFQIIVDIDNGTLTVNKLNGTMNIIGEAGIDW
                     NTDVPLTHLGNGVFSIVTDLFAKEMKFREVAGSWDVNWGIADGQSLELGKPFSLQRDG
                     PNIKIPADGRYKVVVDLGNNSATISASNFPDNLYLVGGGVPAGWDPGSSAPFVKLEDG
                     KFRIYTPITQNGEFKFLQGKSWDGDWGDNKNAPGVLEQNDEQNCKIIDEGFYRIDCDF
                     IAGTWTTLRQEWGIIGSATSQGWNDETPMVKSGNYSWKYTGNLSAGEIKFRANHDWGF
                     NFGDNGADGSLEGDGANIAIPEEGNYEVEMILDPEGYTYTVKKL"
     gene            complement(8452..9285)
                     /locus_tag="PLG24_00445"
     CDS             complement(8452..9285)
                     /locus_tag="PLG24_00445"
                     /inference="COORDINATES: protein motif:HMM:NF025650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusE domain-containing protein"
                     /protein_id="HMZ23376.1"
                     /translation="MKNLFKIIFGALLVTTLFSSCEKDEVKAEFLGGKAPILTASTEV
                     VDMSFANADKLGVDLSWTNPEYQFNTGVSSHDVTYLLEFDLKGADFKTPNRKQLSLEG
                     DLSKKLLVKELNDYMLNQMELSTGTEYDLEVRVTASIDNKYSVLASNVVPLKATPYAI
                     PPKVEPPASGRLWIVGDATPNGWDNPLKPDFKDSQEFTRVSETLYELEIELPGGGGYK
                     LIQEDGVWGSQYHMLAGGTWEGGDIEKKDSDPQFPGPPGPGKYKITVDFQRGKFFAVK
                     I"
     gene            complement(9303..10904)
                     /locus_tag="PLG24_00450"
     CDS             complement(9303..10904)
                     /locus_tag="PLG24_00450"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HMZ23377.1"
                     /translation="MNLKNIIAGLILVLMMGSCANKLDLYPVNDLTPDKAYATAAGYK
                     QVLAKLYGTLTVTGNAGPAGNPDIKGNLDEGSQVGFIRAFFNAQELPTDEAIVAWNDQ
                     TIHDFHHLKYTSSDPFILGMYARPIYNITLINEYLRESTTEKVSSRGITGTDAADIAN
                     SRAEARFLRAFNYWVMMDLFGKSTFITEADQVGTALPKEIGRTELYNYIVGELKAIDT
                     ELKPARSAEYGRVDQAAAWALLARIYLNAGVYTGTTHYAEAIDYASRVINAGYTLQPD
                     YARLFMADNDSQKDEFIFTINCDGLHGKSYANTTFLVHAACSDDFAEYGTNNGWGGYR
                     ATKGLADKFSDLSGNTDKRALFTTSVFGTSSGQIAIPSVGDFKNGLHVKKFVNVRSDG
                     GAVSDPTREFVDTDFPVFRLAEMYLIYIEGVLRGGGGDAGTALEYFNTIRTRAWGGST
                     AGNITAGDLTLQMVLDERARELYWEGHRRTDLIRYDQLTTGEYLWPWKGGVANGTSVD
                     AKYNLYPIPNANRASNPNLTQNPGF"
     gene            complement(10932..13889)
                     /locus_tag="PLG24_00455"
     CDS             complement(10932..13889)
                     /locus_tag="PLG24_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HMZ23378.1"
                     /translation="MKQCLITLFALLSFSFLQAQSVISGSVKDAKGVGIVGATVLVTG
                     SNNGAATDIDGNFRIDNVSGTAVSLTVSSVGYTTQVVKVDFAGRNSASVQVVLVEDYK
                     LLDEVVVVAYGTRKREDLTGSVTSVGEKDFQRGNIATPDQLLTGKVAGLQVTSGGGSA
                     GGGSRLRIRGGASLNASNDPLIVIDGIPVESNGIAGSGNFLNTINPDDIESISVLKDA
                     SATALYGSRASNGVVIVTTKKGSTGKLQWNFNTQLSVSQVAKKVDVYTGDQIRDIIKA
                     TGNQAYINLLGTANTDWQDEIYRSAFGNDNNLSVGGSVANIPFRASVGFNNQDGVLKT
                     NNFKRMSASLNINPSLFDDDLKLNIALKGTQTRNTWADEGAIGAAVSFDPTQPVHAAN
                     KFGDYYEWLQNENTPHPLSTRNPLGLLELRDNTSKVNRFVGNLQADYRLPFLPDLHVL
                     ANVGMDRAFGQGDDIIKALSATNYQTGGRRVHYEQGKTNSLLDLSLFYEKSFGKNSSV
                     DILAGHSYQDFYTDVYNFPSYPEVGDVAIPGTIPTFDSDRPEFRLESYLGRVNVNLAN
                     KYLLTASIRRDASSKFSKDNRVGYFPALAAAWKLKDEFFSGSNTFSELKLRLGWGVTG
                     QQDIGSYYSYLPVYNVSSSTAQYQFGNTFYPFLRPNAYDANIRWETTTTSNVGLDFGF
                     ADSRITGSIDVYKKKTKDLLSVVPVAPGSNFDITLLTNVGNMENKGVEVTVNTIPLKS
                     EKMIWSLGFNVTYNETKITNLLRNQDPSFKGINVSGIGGGTGNTIGKFEVGYAPYTFF
                     VYKQVYDAKTGAPIEGLYEDLNRDGKVDDNDRYLYTKPAPDFMLGLNTQVIIDKLTIG
                     IAGHGAFNNYLYNNFASQGAALRRIEDPLNYISNGSRIYDDTRFVNNQYLSDYFIENA
                     SFFRLDNINIGYNIGRIFRDKASLRLSANIQNVFVISKYRGLDPEIASDSGVDNNIYP
                     RPRIFSIGASLDF"
     gene            complement(14584..15408)
                     /locus_tag="PLG24_00460"
     CDS             complement(14584..15408)
                     /locus_tag="PLG24_00460"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HMZ23379.1"
                     /translation="MWNYIRQSIARKRARRITRKYDTVIDHFEIEGIGRIDFANWTNP
                     LVEKKVISRSQVDFFAKFLKPGDLAVDIGGNVGHMTIAMSLAAGREGRVVSFDPNPFV
                     YDILVKNAGLNPGLTRIDTFPYAIADHEGEFYYNSSEASFNNGGISEDTNSRHGRFAL
                     DHKIKGIKLESLLEEKYPEELGRLRLIKIDTEGYDKEIIRSIRPILQKHKPTVITEVF
                     KKNDASARAEQFELLSSCGYTMYYFSDFVADAEVIRIENKDQMMQWKHFDMYAICN"
     gene            15980..17227
                     /locus_tag="PLG24_00465"
     CDS             15980..17227
                     /locus_tag="PLG24_00465"
                     /inference="COORDINATES: protein motif:HMM:NF025430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HmuY family protein"
                     /protein_id="HMZ23380.1"
                     /translation="MRRFLTIFLTISFFSVAYTQSYDEVSTGAQYSQQAYYNLSTGSV
                     QIVNNNAWDIAFSNIGQTDAGIFINEAASLAENPVKLFVALDNDWSTPITDASIFIDD
                     VQLFNSENNWTIGAFNETRNPDDPTDFGWGQYNPQNHQIIGNEVYVVKKRNGDFIKFK
                     VDAYRNGYYHFRYANLDNSNEVQDSVARNTNGPKSLVHYSFDSKGIVKIDNGYDLVFT
                     RYITSLEDTPGHFINYSVTGFLLAPGTLAVVADSVDVATVKESDYSLAYSSAPTTIGH
                     DWKSFNLTSGWQIDEDRANFVKTKNGETYKLVFVDFSGSSSGTVTVERTKLATSKVNP
                     DLQFPGMTLYPNPAQNVLYIDSDKPEPVRVSVINSLGRVVLEEATDTRSSITLPSDIT
                     PGMYHIVVQGKERRSAFSIVISK"
     gene            17293..19341
                     /locus_tag="PLG24_00470"
     CDS             17293..19341
                     /locus_tag="PLG24_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ23381.1"
                     /translation="MRSLFPIFIIIAFPLVVTAQSHTTEAFLDSIERSVVLEEYVHTA
                     EYKPTHVKKALHSIEVLKTESLNRRGVTNLEEALSIIPSVRYDYDPILGTQLRLRGIS
                     AANVAILVDGVPVIGRLDGAINISQIPLINVKQIEIIEGPLSTIYGNNAAGGVINIIT
                     TDSQSKKWSLGGSSQIESIGTQNHQLDLGLQTKKWYVDMHGRFFNYDKYPIDSLRLLE
                     KYTLPDGSISTRSKYPWNPKLQMSAGTTVKYKVSADNSLVFKYDLSKESLLDYGPVKR
                     PAFKPYAFDDRFLTSREDISFMYDGLLFHKLYVELKSSYNRFGRHVDNLLFQFQENQY
                     DETQTKSDTTRFRSFFNKLNLSMRITPQLEVLGGVNHTFDAGKGGRIKVENPNEHEAI
                     MSEWAYFSEVRYSLFKRFTFSYSGRFLTHSEFGNNYTSGIKAKADLGKNIVLRASYAQ
                     GYRTPDLKERYIEFIDVNHNIIGNPNLQPEKSNDIQLTIQYNPYQWMNAKLNAFRTEI
                     NDKISLFQYAPLKYNYSNVDIYTVSGLKWDMSGSWKQLNWALGASLGFWNTIIESEEK
                     PYKGKVLDYNATLNYTIPKVKIAAMLNYRYNGPQPLFSVDFGDVNISEISAASFLDFS
                     LNRYFWKERLQLVLGCKNILNTTYASVSGADNSGSHRVSGFQFINPGRNYFVKLGINL
                     "
     gene            complement(19462..20034)
                     /locus_tag="PLG24_00475"
     CDS             complement(19462..20034)
                     /locus_tag="PLG24_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HMZ23382.1"
                     /translation="MNTTEVFYPKSLADWRGWLTENHLSKQSVWLVFYSRTSDKKSIT
                     WSGAVDVALCFGWIDSKKIKIDHETSHQFFSKRKEKSTWSRINKEKVQQLIDQGLMTV
                     AGYESIEIAKQNGSWTILDEVEKLIIPADLNEAFGKHKGSKAYFQGLSKSSQKMMLSW
                     IVLAKRPETRLKRIEEIAILAGQKQKPKQF"
     gene            complement(20037..20189)
                     /locus_tag="PLG24_00480"
     CDS             complement(20037..20189)
                     /locus_tag="PLG24_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23383.1"
                     /translation="MDIASGNHWESMDTDVPCVRKHGIKLATTLIDLKELFSTWTVGV
                     EPTEAK"
     gene            complement(20419..20997)
                     /locus_tag="PLG24_00485"
     CDS             complement(20419..20997)
                     /locus_tag="PLG24_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23384.1"
                     /translation="MINRLTELIWLRWPAVIVSLLLLSCKAEVKQSGAASNPFFQVSD
                     YILDDYRRERSEKALFKTITINGQTDTLLLTDSLNAGETIIMDKLNLNNPDWIPKYKA
                     DTVFGVYGDADRYQYRALDEDLPVQSIEVNMEKGTVSNIRVHNRRRSLINRHDQFLTY
                     IPHTGYRLLSLQRMWNGDTLRMEVSAEFRMMR"
     gene            complement(20994..>21522)
                     /locus_tag="PLG24_00490"
     CDS             complement(20994..>21522)
                     /locus_tag="PLG24_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HMZ23385.1"
                     /translation="PHVTFFFSGGREEPFSGEFRTLIPSPKVATYDLQPEMSAQGICQ
                     AVLDAMDDKKPDFICVNFANTDMVGHTGVFEAAVKAAETVDGCLGRITEKALALDYRM
                     IIIADHGNSDYMINEDGSPNTAHTKNPVPCIFVGNDTQGLTLGEGRLADVAPTILFSM
                     GIKQPEIMTGQNLVQ"
BASE COUNT         5913 a         5166 c         4474 g         5969 t
ORIGIN      
        1 gaattgcctg gaattgttgg ctgccgataa aaatgtcaaa gaattgtttt ttgttcccgg
       61 agagctgaaa atagaacccc gacttctgga caagtccctg aaaggaactg tcagggcccg
      121 ggtgtcgtgc tattccggct ttaccttacc atctatcctt caacaccgtt tggttacatt
      181 aaaacttaga gatcatttca cggcagaaag aagttatttc gatgtccatg tcgatcataa
      241 tgccctcatc ctgctgtcgg aagaacagtc ggtagaatat atttcctttg ccccggagac
      301 ctttatgccg atcaatgaca aaaaacgatc ctttgaagga atacagggcg tgaatgcatc
      361 ggccggtgca ggtggtttag gcctcaatgg tgcaggtgtc accataggtc atggtgataa
      421 tggcagcttc aaccatatcg atctgaatga tcgcgtaatt aatttcagca tttacaccgg
      481 aggcaaccac agtgcacata cagcaggcac tgaggccggt gcgggactgc tagatcccag
      541 gcgaagaggg ctggcatcag atgctaccgt ccttaatgat gactttttcg atatcatcac
      601 cgaagcttca tcgtactacc tgggaaataa tatgcatgtc accaacaact catatgccac
      661 ccttgatgct gtccctaatt gtacgcaggt aggcgattac aatatttaca gtcaggaaag
      721 tgacaatagt acgattacct atcccgactt gctccacgtc tttgccgccg gcaattacgg
      781 tactacatca tgcccgccca atggagatta taacaacctt cccgacggac cacaatgtgc
      841 aaaaaatacc attgccattg gcgctctgat ttccggagaa ggaaatgctc cggcaaatta
      901 tacctctaaa gggcctacgg atgataatag actcaagccg gagatatcta ccataggaac
      961 tgccacaagc tgtatcaatg gtaacgctta tagcggctcc tccggcacca gtatggcagc
     1021 cccatccgtt actgcggctg tcgcgctgat gcaggaagcc tataccggaa tacacggagg
     1081 gacattcccg cctgctgccc ttatcaaagc catcctttgc aatacgtccg atgaattagg
     1141 tgccgaaggc cctgatttca ccaccggata tggcaagcta aatatcaaca atgccgtcag
     1201 gcacaccctc aatggcaacc atgttttgaa tgccatctct cagggtagca cccaggatgt
     1261 tgctatcacc atcccatccg gcactgcaaa acttgctgtc accctggcct ggactgaccc
     1321 tgccggcaat cccctgatct taagccaact ggtcaatgac cttgacctgg aagtcatcgg
     1381 accttccggc acaactttgc cctggtttat tgccggcatc ggacaacctg tccaaagagc
     1441 tgttgacaac ctcaacaacc tggagcaggt aactatactc aacccggtct ccggcaacta
     1501 cacgctccgg gtcaagggta cttcggtagt gggtagccag cagtatgccc taacctatcg
     1561 cacaatcacc ccggatatta atatacaagc accctttggc gaagaaaaat atctggcagg
     1621 tgagtctgtc tacattcaat gggattgccc ggttgatcct tcaggcagct ttctgttgga
     1681 gatatccacc gatggggggg ccacctggtc cactatcacc aacaaggcag ccacagccag
     1741 aaatcacaac ttttccatgc ccaatgtcaa taccaggcag gcacaggtta ggatttcggc
     1801 taccggcctg acgaccgacg tgtcatccga ctttactgtt tctggtgtgc ctgccggcct
     1861 ggcggcaacc ataccctgcc agggctatgc caacctgacc tggaatgcag tatccaatgc
     1921 tacaggatat aaaatttata aaaaagtcgg ttcggtaatg gtttttgtgg acaatgcctc
     1981 cactaattca taccaactat ccgggctgac acccaatgtg caggaattga tcggggtaac
     2041 tgccactttt cccggagata tcgagagtcg tcgctccggc ggcatctttg tcaatgcagt
     2101 tggcggagct tgtccatggt ccaatgacat ggaaatggta agcctgttga cccctgtatc
     2161 cggacgtaaa tatacatcgg gcgaattgac ttcttcacaa gtaatcacaa ttcggttgaa
     2221 aaacttaggt tcatcgtcgg catccggctt tcaggtatat tacaaggtca atggtggcag
     2281 tgagattatt gagaactata ccggttccat cgcgaccaac acgaccgctg attatacatt
     2341 tacaacaact gccaatcttg cagccatcgg aaattattcc atcgaagccg gtatttgggg
     2401 agcgcctgat gcgccattaa ttgctaacaa tatactttct accctggtaa agcatgttgc
     2461 caacgatccg gtcaccttga cccaggattt aaacacaacc tataaggtcg actttgaaag
     2521 tgcgacatca gctacctatt cacaatctta ctttgccatg gatggaatac cgatgtttga
     2581 ctttgtggca catagtaacg acagccgtgc ccggacatac attacttcag gcttagcacg
     2641 atcaggcacc aaagcgcttt cactcgattc aaagactatc agcaccatgg ctgtcaatga
     2701 tgccatcatg accgtcaatc tgtccaacta ttcaagctcc ggcaccgtgt tgtttgactt
     2761 ttcctacctc aatcacgatg catcactcag cgtcaatgat acggtatgga tcaggggtaa
     2821 tgacagttct ccctggctgc cattatatgc actggccctc aatcaggctg caccgggaga
     2881 gtacaagaat gtgtacgggc ttaatctaac atctttccta cagaatgccg gtcaggcgct
     2941 atcttcatca acccagatcc ggttcggcca gtccggcaat ggccagattg tgtccaggaa
     3001 tgccctacaa ggatatacct tcgatgatat tgttcttcac ctggtaagca ctgatgccgc
     3061 tttgacccaa attgtctctc cgtcacgcaa ggcttgcgaa tccggctcaa atgcccttat
     3121 caccatccga attgacaaca actcccagat ttccttatct ggtgtcaagg catggtacag
     3181 aatcaacaac aatgctgctc aggggccatt ctccattgga acaattcctg ccaatggcaa
     3241 tttgcagttt tcttttccac ccgtggactt cagtgcctat accgattata cgctcagtgt
     3301 ctggatcaat gaaccgtcag acacttatct gctcaatgac actatcaaga attataaatt
     3361 ccacaattct cctttgatca atacttttcc acatctgcaa acctttgaaa cagatgatgg
     3421 cggatggtat gcatacgggc agaacagttc ctgggcatgg ggcactcccg gcaaaaccaa
     3481 gatcaataag gctgccaatg gcaccaaagc ctggactacg ggcttgaccg gaccctataa
     3541 caacctggaa acctcttatc tggagtcgcc ctgttacgat ctgtcgggct tggcttcaga
     3601 cccgatgatt tcgttctcga tggcccatga gatagaaacc aactacgaca aagcctggct
     3661 ggaagtgact ggcgatggcg tcacctggat caagctgggc agtctcaatt caggggtcaa
     3721 ctggtataat aagcaatcct actgggacaa tgcattgtca tattaccatg ttgcttctca
     3781 tacaattccc cgaagtctgt tgggcagtgc cacatcggta agcttccgct ttgtcttggt
     3841 gaccgaccag ggactggtcc tggaaggact tgcaatcgat gacatacata tcttcgagcc
     3901 aaagtcgatc tataccggta ataaccagac tgtcactgtc tctccacttg tatccggctg
     3961 gaccgatgtg tattccgggc aaaacatgat tgcttctctt cgaccgaata atagtcagtc
     4021 gttgggaaat actgatgtcg ctgtgtactt caatccctca aacgaccaac ggcatgacct
     4081 caaccagtat tatctcgatc gtaactttac cattgtaccg accaatcccc tttcttcagg
     4141 aacagttacc gtccggctgt actttaccga tgaagaggtc aacaaaagcc gcaatgcccc
     4201 ctccggatac gataaaccga ccgacgctta cgagttggga gtaacacaga tttcatccaa
     4261 tagcgacgat ggcatatacg gtaatgaaag taatccgtca gtcaaatatt tctcctgtct
     4321 caatactcag actaccccat ttaataccgg gtattatatt gaatttgatg tgaccggatt
     4381 ttctgaattc tatctgaatc cgggaggcat caacaacaat cagccccttc ccctcaagat
     4441 actttccttc gaggcatcag ctctcacaga acagcatgcc gcaaggcttt cttatcgact
     4501 tgtcgatggt gtcaatgtcc gttccgtaga tattgagcgc agcacggatg gaaggttctc
     4561 taacgcacgt attatagcaa gttccgttta cacagaggac ttaaagatat ttattgacaa
     4621 tgtcccgccg acagtggaaa atgtctatta tagattaaag ataactgacc gtgacggggc
     4681 cgtcagctat agctttgtaa gaaccgtaca ccttccggat gaattatcgg tacaggtgtc
     4741 acccaatccg accaggaata cacctgttaa ggtcctggtt tcaggcagtc gattcggcca
     4801 atctgcatcc gtcgagcttt ctgatatcaa cgggcagact tgtcgggcgt tcaggtttgt
     4861 gccgaatggt gatcccttat tcatgaacac ggaaggattg acgtccgggg tatatatcct
     4921 gacagtaaaa gttggagaag gcttgaaaca ggtgaaggtt gtagtattgt aaaaaccgga
     4981 acataatctc ctgttcctgg cgctaatcag gagatgcaat tggcaacctg ccaaactctt
     5041 ttgtcccggc aaatttactg tttgccaact ctcaggactg accttttata gctttaggta
     5101 ttcacggttc gccctgtata cccttagctt cttttacctt actgaacact ttgcccgaaa
     5161 gaaaatgcag atacaaatac tcctcatatt ccagcacata cccacctcct tcaaagagat
     5221 tgatatcttc agtaagtgat cgggtgctga ctcccgccat acaattgaaa aaatggtgca
     5281 tcaggctaag aatggttttc ttccaccagg caccataccg attattgatg gaaaaatcgc
     5341 aattcaacca ccggcctcca tgcattagaa gaccgtccaa ccgatcaaaa agcgccctgc
     5401 actcttctgc ctcaaaatta tcgaacagaa atgatgtaaa gatgacatcg tattgtccgg
     5461 atgacatgaa atcaaatatg ttttcctgcc ggaattgtac attgaatggc cctaaccggc
     5521 gtccggaagc caatcgcaac atacgtgccg agttgtcaac aaagtcaatg tccacaccct
     5581 tcaaagattg tatatagtca tcttccaata tccagccgga tcctccaccg gctatgagca
     5641 ccctgtccca actccggata aatcttaata cttgccgttg tatcctttgt tgcttcccca
     5701 gaaagacaat tctactcgtt atgtcataga tacctgctac cctgtcataa ttgatacgtt
     5761 ctccactcat aacaatgccc caatgatacc gcacaatgcc ttgaatatca atacaccgtc
     5821 gatgatgacc aaatagaaga aaatagacct gggcctcaac attgaaaatg ctatagccat
     5881 tgtagccagg aaaggcagta gattgaaaag ataccgggcg agggagaaat tcatctcccg
     5941 ggcaaacaaa gaaaaggaaa tcatgcccgc aagcagcaaa ggaaacagaa ttcggtatat
     6001 tgtcctgcgt atgccgaacg aaaccacaaa tgttctaagc cgggcattga ggtcggtagg
     6061 ataatccttg atatcgaaca tgattgaatt gacggtacaa aacatccagt tctttataaa
     6121 aaaccaaagc cacagataga tatccagatc gatggaaatg cctcgggcat tcaaaaaaat
     6181 gaccggaagt gctgtggaaa agtaccccca cacccaacca atgataaatg atttcaacca
     6241 gtggctctgt cgtatatcga acccgaagta tttcctggat ataaggccat agtagaacaa
     6301 agccatacaa aaggttgtac cggataatag ataccatggc cagcttatat tgctaaggta
     6361 gaacttacct gaaatcaaag gaataaaagt ggccaacatt acaataccta tcatgcccac
     6421 ttcccattgc cacagcacct tggcatttct gacatagtac ctgcttcgtg gattttcgtc
     6481 cggtgcctcc ttgcctacct tgagataggc ataggtataa tatgctattg gcaggaaaaa
     6541 gataatcaga taatcgctcc aggcagcaaa aggcaagctg acctgaaagg cggtttcaat
     6601 acacagtcct acggcaagcg ttccgacaag ataattgcca aataaaacgg attcccacaa
     6661 ccgaccgatc ttcatatcca aatgccaaaa taaaaaaagc aggctatctt tcaataacct
     6721 gcttgtaaaa ttaactaaca atcaaacatt aaagtttctt cactgtatat gtatatcctt
     6781 cgggatccaa aatcatttct acctcgtagt tgccttcttc cggtatggca atattggcac
     6841 catctccttc gagcgaacca tccgctccat tatctccaaa gttgaatccc caatcgtggt
     6901 tggccctaaa tttaatctca ccggcagaca aattcccggt atacttccag gagtagttgc
     6961 cgcttttgac cattggcgtt tcgtcattcc agccctgaga ggtggcactt cctatgatgc
     7021 cccactcctg acgcagggtc gtccaggtgc ctgcaataaa atcgcaatca atccggtaaa
     7081 acccttcgtc tattatctta caattttgtt cgtcattttg ttcaagaaca ccgggagcat
     7141 ttttattatc accccaatcg ccatcccagc tcttgccttg taagaatttg aattcgccat
     7201 tctgcgttat tggggtataa atcctgaact ttccatcttc caactttaca aatggagccg
     7261 agctgccggg atcccagcct gccggcacac caccacctac aagatagaga ttatccggga
     7321 agttggaggc actgatggtt gccgaattat ttcccaaatc gaccacaacc ttatatcgtc
     7381 catctgccgg aatcttgata ttggggccat cgcgctgcaa gctaaacggc ttgcccagtt
     7441 ccagactctg cccatccgca ataccccagt tgacatccca gctgccggcc acttcacgga
     7501 atttcatctc tttggcaaaa aggtcggtta caatggaaaa cacaccattt ccaaggtgag
     7561 tcaagggcac atcagtattc cagtcaatgc ctgcttcccc gatgatattc atggtaccat
     7621 tcaacttgtt gaccgtcaag gttccgttgt ctatatcgac tataatctgg aacaggtcgc
     7681 tcccactgcc atccggcaac ttgaagttag gaccgttgag cttgatcccc atacctttcc
     7741 caaattcaaa tgtttcatca tctgccaggc cccagttgat gtcccagctg ccggcatcgc
     7801 gcctgatctt aaactctttt ccccactgaa gttcagtcac cacggcaaac ttcttggttc
     7861 caatcagatt cataggcaca tcaacatccc agccttttgc cgcttcaccg atgatattca
     7921 tggttgttgc gtcgatggaa gtcgatggtc ccttatatgc tgtcatgttg atggtcagga
     7981 tattggaata gtagcgctcg ttattattgc gataagatga cgctactctc acctccatat
     8041 catgcggctc atcatacaga aaaccaaatc ccgcaagaat attgttcaat tcacgtccgg
     8101 tgaaattctt cttgaactcg cctatgacaa ccgactggct agcattggca aagttttggc
     8161 ccttttctgc aatttctatc aaaaacttat aattggcctt ttctgtagaa tactgaggat
     8221 cagaccagtt cagtgtaagt accacattgt cggagtccga tgccggaggg gctacctgca
     8281 gggacgaagc tgtaattgta acagcctttc cgttgctata ggtctcgagg tcatcaaatt
     8341 ttttacaagc cgtaaaaaag aagctcagac aagcagttaa aacaaaaaga ttatatattt
     8401 ttttcatctt caattatttg atcaattaac attatatctc cgaacaggct tctatatttt
     8461 cactgcgaag aatttacccc gttgaaaatc aacagtaatc ttgtatttgc ccggtcccgg
     8521 aggccccggg aattgagggt cggagtcttt tttctcaatg tcgccgcctt cccaggtacc
     8581 acctgcaagc atatgatact gtgagcccca cacgccatct tcctgaatca gtttataccc
     8641 accgccaccc ggtagctcga tttctaattc gtaaagagtc tctgaaaccc ttgtaaactc
     8701 ttgagagtcc ttaaaatcag gcttaagagg gttgtcccag ccattcggcg tggcatctcc
     8761 cacaatccaa agccgtccgg aagcaggtgg ttccacctta ggcggaattg catatggggt
     8821 ggctttcaat ggaacaacat tggatgccaa aaccgaatat ttgttatcga tcgatgctgt
     8881 cacccgtact tccaggtcat actctgtacc tgtggacaat tccatctgat tgagcatata
     8941 atcattgagt tcctttacca acaatttttt actcagatct ccttccagcg acaattgttt
     9001 tctgttagga gtcttgaaat cagcaccttt aaggtcaaac tcaagcaaat aggtaacatc
     9061 atgagaacta acacccgtgt taaactggta ttccggatta gtccagctga gatcgacgcc
     9121 caatttgtcc gcattggcaa agctcatatc aaccacctcc gtactcgctg taaggatagg
     9181 cgcttttcct cccaaaaact ctgcctttac ctcatccttt tcacaggacg aaaaaagtgt
     9241 tgtcaccagg agtgcgccaa atattatttt aaataaattt ttcattttct gcattttaga
     9301 aattaaaaac ctgggttttg agtcagatta ggatttgaag cacggttggc gttcggtatc
     9361 ggatagaggt tgtatttggc atccactgat gtcccgttgg cgacaccgcc tttccatggc
     9421 cacagatatt cccctgtagt aagctggtca tatctgatca ggtccgtcct tcgatgaccc
     9481 tcccaatata actcacgtgc tctttcatcc agaaccattt gcagggtaag atcgccggca
     9541 gtaatatttc cggcagtgct gccaccccat gccctggtgc gtattgtgtt gaaatattcc
     9601 agagcagtac cggcatcgcc acctccacct cgcaatacac cttctatata gatcaggtac
     9661 atctcagcta atctgaacac tggaaaatcg gtatctacaa attcacgcgt cggatcggac
     9721 acggccccac cgtcagaacg aacattgacg aattttttaa catgcaggcc atttttgaag
     9781 tcgcccactg aaggaatagc aatctgccct gagctggttc cgaatacaga tgttgtaaac
     9841 aatgcccttt tgtcggtatt gccggacagg tcactgaact tgtcagcaag ccccttagtg
     9901 gctcgatagc cgccccagcc attgttggtg ccatattccg caaaatcatc actacaagcg
     9961 gcatgtacaa ggaaggttgt attggcatag gatttgccgt gcaagccatc acaattaatg
    10021 gtaaatatga actcatcctt ttgactgtca ttatcagcca taaacaatct tgcatagtcc
    10081 ggctgcagag tatatccggc attgattacc ctggaagcgt agtctattgc ttcggcatag
    10141 tgcgttgtac ctgtatacac accggcattc aggtaaatac gcgccagcaa tgcccatgct
    10201 gctgcctgat caacccggcc atattcagca gacctggctg gcttcagttc agtatcgatc
    10261 gctttcaact caccaacgat gtagttgtac agttctgttc ttccaatctc ctttggaaga
    10321 gctgttccaa cctgatctgc ttcagtaatg aaagttgact tgccaaacaa gtccatcatc
    10381 acccaatagt taaatgctct aaggaaccgc gcttccgcac gactgttggc gatatctgcc
    10441 gcatcagtac ccgtgatgcc tcttgatgag accttttcag ttgtcgattc gcggaggtat
    10501 tcattgatga gcgtaatgtt atatatcggc ctcgcataca tacccaggat aaaggggtct
    10561 gaagaagtgt acttcaagtg gtggaagtcg tggatagtct ggtcattcca ggcgacaata
    10621 gcttcatcgg taggcaattc ctgcgcattg aagaaggcac ggataaaacc tacctgtgat
    10681 ccttcatcca gattgccttt gatgtccggg ttccctgccg gaccagcatt gcctgtcaca
    10741 gtcagcgtgc cataaagctt ggccagtacc tgtttgtacc ccgcggcggt ggcatatgcc
    10801 ttgtccggtg tcagatcgtt gaccggatac agatccagtt tgttggcaca agagcccatc
    10861 atcaacacca gtatcaatcc ggctattata tttttcaaat tcattttaat catttcttgt
    10921 tcatttaaaa tttagaaatc taagcttgcc ccaattgaga aaattctcgg acgcggataa
    10981 atattgttat caacacctga atcacttgca atctcgggat caaggccccg gtacttggaa
    11041 atgacgaata cattctgaat attggcggac aatctcaagg atgccttgtc tctgaatatg
    11101 cggcctatgt tatatccgat attgatattg tctaatctga agaaagaggc attttcaata
    11161 aaataatcgg acaaatactg attgttgaca aatctggtat catcatagat acgtgatccg
    11221 ttggaaatgt agttcagcgg gtcctcaatc cttctcaatg ctgctccctg agaagcaaag
    11281 ttgttgtaca gatagttgtt gaatgcacca tgtcctgcaa taccaatggt caatttgtca
    11341 atgatgacct gcgtgttcag accaagcata aagtccggcg ccggcttagt gtacaggtaa
    11401 cgatcgttgt catcaacctt cccgtctcga ttcagatcct cgtacaagcc ttcgatcgga
    11461 gctccggtct tggcatcata tacctgctta tagacgaaga aagtatatgg agcgtacccc
    11521 acttcaaact ttccaattgt atttcctgta ccgccaccga ttccggaaac attgataccc
    11581 ttgaaagagg ggtcttgatt tctaagcagg ttggtaatct ttgtttcgtt atatgtaaca
    11641 ttgaaaccga ggctccatat cattttttca ctcttcagtg gaatggtgtt cactgtaact
    11701 tcaacgcctt tgttttccat gttgcccaca ttggtgagga gcgtaatatc aaaattagag
    11761 cctggagcta ccggtaccac actcagcaga tccttggtct ttttcttgta gacatcaatg
    11821 ctacctgtga ttctgctatc cgcaaaacca aagtccaatc caacattgct tgttgtcgtg
    11881 gtctcccatc ggatattggc atcgtatgca ttagggcgaa ggaaaggata gaacgtattg
    11941 ccaaactgat attgagcagt tgaactactc acattataca ccggcagata ggaataatac
    12001 gatcctatgt cttgctgccc tgtcacaccc catccgagtc tcagtttaag ttcggaaaaa
    12061 gtattggagc cgctgaaaaa ctcatctttg agcttccagg ctgctgccaa tgccgggaaa
    12121 tagccaacgc gattgtcttt tgagaatttg gaagacgcgt cacgacggat agaagctgtc
    12181 aacagatact tgttggcgag attcacgttg acacgaccca aataagattc cagcctgaat
    12241 tcagggcgat cactatcaaa agtgggtatc gtgccgggaa tggccacgtc tccaacttca
    12301 ggataggatg ggaagttgta tacatcggta tagaagtcct gataactgtg tccggccaag
    12361 atatccacgg aagaattctt tccaaaggat ttttcataga ataatgacag gtccagcaag
    12421 gaattggtct tgccctgctc atagtgaact cttcgacccc ctgtctgata gttggttgcc
    12481 gacaaagctt tgataatatc gtctccctga ccgaatgccc tatccatgcc cacattggca
    12541 agaacatgaa gatccggcaa gaatggcaat ctatagtccg cctgcaaatt tccgacaaat
    12601 cggttgacct tagaggtgtt atccctgagc tccagaaggc ccaacggatt acgtgtggaa
    12661 agggggtgag gcgtattctc gttctgtagc cactcataat agtcgccaaa tttattggcg
    12721 gcatgtaccg gttgagtcgg gtcaaacgaa acagcagcac caattgcccc ctcatcagcc
    12781 catgtgttgc gtgtctgagt ccctttcagt gcaatattca acttaaggtc atcatcaaat
    12841 aatgaaggat tgatattcag actggcagac atccttttga agttgttggt ctttaaaaca
    12901 ccatcctgat tgttgaagcc gaccgaggcg cggaaaggaa tatttgctac tgatccgccc
    12961 acactgagat tattgtcatt gccgaatgct gaacgataga tttcatcctg ccagtctgta
    13021 tttgcggtac caagcaggtt gatgtaggct tgattaccgg tagccttgat gatatcgcgt
    13081 atctgatcac cggtatagac atccactttc ttggcgactt gtgagacaga aagttgcgtg
    13141 ttgaagttcc attgtaactt tcctgtacta ccctttttgg tagttactat cactacgcca
    13201 ttggaagcac gtgaaccgta taatgcagtc gcggaggcat ctttcagcac agagatggat
    13261 tcgatatcat ccggattaat ggtgttgaga aagttgcccg aacccgcaat accattgctt
    13321 tcaaccggaa taccatcgat aacgataaga ggatcgttgc tggcattgag tgatgctcca
    13381 ccacggatac gcaacctgga gccacccccg gcagaacctc caccactggt aacctgaagt
    13441 cccgcaacct ttccggtcag tagctgatcc ggagtcgcta tgtttccacg ctgaaaatcc
    13501 ttctctccca cagaagtaac tgatccggtg agatcttctc tttttctggt accataggcg
    13561 acgacaacca cttcatcgag aagcttatag tcttcaacca gaaccacttg aacagatgct
    13621 gaatttcggc cggcaaaatc aaccttcacc acttgtgtgg tatagccaac agaagaaaca
    13681 gtgagggaaa cagcggtgcc ggatacgttg tcaatcctga aattgccatc gatatcagtt
    13741 gctgcgccgt tgttggaacc cgtcaccaaa acggttgccc cgacaatgcc aacccccttt
    13801 gcgtctttta cgctgccgga aataacggac tgcgcttgga ggaaggagaa ggacagcaga
    13861 gcaaacagtg taattaaaca ttgcttcata aaataatttt ttgcttgaat aatttttgtc
    13921 ccaaataatg attttataat gtgtactttt tacacattat attcagcgaa gataagccgc
    13981 attcactgaa aactaaaata ttttgacctc ctttttaaaa caaaaaacaa gttaattccc
    14041 aaaatgataa attataatat caaaacatcg aaaaatacag caataaatta ttgacaatta
    14101 tttgtttgtc gaattaatcg aatacttttt agaataaaat ttgcttatgg gcgcgtttac
    14161 actaaccagg gctcggtcgt ggtgaaaaat tttcaacatc aatcccggga attcaaaata
    14221 ggttgaccgg gagtcaatct aaacatgttt ctttcttatc cctcgtataa tcacccttaa
    14281 taagccccct ccggaggcaa caaaggaagt ttcgtccact ttcaaaattc cccgcctccg
    14341 ttcaatattt tgtcaatgtc catagacgaa atcaggaggc tctgcggcat catgccttca
    14401 attgaagttt gacaaaatct tgtaaaaaat aattgtaccg ggagcaaaag gcctgcccaa
    14461 catgccaaaa aagttttccg acatcaaaaa cagaatgcct taaggaccgg taatggctaa
    14521 acgctcctat tattatgagc ccggacgtct gtttgccttt ttgtggcacc tcttgtccgg
    14581 cggtcaatta catatggcat acatatcgaa atgcttccat tgcatcatct gatccttatt
    14641 ttctatcctg atcacctccg catccgctac gaaatcggag aagtaataca tggtataacc
    14701 gcatgaggac aacaattcga attgttcggc tcttgctgat gcgtcgtttt ttttgaatac
    14761 ctctgtgatt actgttggtt tgtgtttttg caaaattgga cggatagagc ggatgatttc
    14821 cttatcataa ccttcggtgt caatcttgat cagtcttagc ctgccaagtt cttccgggta
    14881 tttttcctcc aacagcgatt ccagcttgat acccttaatc ttatgatcca gggcaaaacg
    14941 tccgtgacgg ctgttcgtgt cttcggatat cccgccatta ttgaaggatg cttcggaaga
    15001 attgtaataa aattcacctt catggtcagc gatggcataa ggaaaggtgt cgatccgggt
    15061 caatccggga tttaatccgg cattcttcac cagaatgtca taaacaaaag gattggggtc
    15121 aaaactgacc acccggcctt ccctgccggc agctagcgac atcgctatcg tcatatgacc
    15181 aacattgcct ccaatatcca ccgcaagatc tccgggcttt aaaaatttag caaagaaatc
    15241 aacctgactc cgggatatga cttttttctc caccagagga ttggtccagt tggcaaagtc
    15301 gatcctccct attccttcaa tttcaaaatg atcaattacc gtatcgtatt tccgggtgat
    15361 acgacgggcg cgctttctcg cgatggattg tctaatataa ttccacatag gccggttttt
    15421 taatgaaaat cggacaatag agtacatcgt ccaattcaag tgccaagata ctgacaaatt
    15481 gaatcgtgcg gaatttttct taaccggaga ttgaaaataa aatgatgata tgcaaaggag
    15541 ttccggcaga atttaccttc cccgaattaa aactatgttg tgaaaagatc ggtttggttc
    15601 gttttgtttt tcaataaaaa cggacctaac catttatttg tcaattggtt agtaggcccc
    15661 ggaagagaaa aagcaagtag gtagatgttt aatagcgcag cattctcttg ttccatgtat
    15721 ttcacaaaat tccttcgttg atttgttgga aaaaaaaata aattttacaa tgatattcca
    15781 caatcgtgag actgctattg tctttgtcgt tattattttc ctacctactt agatttgacc
    15841 ttccgattca agttccttaa cttttacaga tttaaatttt cggaacttat ttatatttaa
    15901 tctaaataaa attattagct ttgcgccatg caaaatcatt gaatgattag ttaatttata
    15961 atttaaaaga gtaatattta tgagaaggtt tttaacgata tttttgacta tttctttctt
    16021 ttctgttgcc tatacgcaat cttacgatga ggtgtcaaca ggcgcacaat acagccagca
    16081 ggcatattac aacttatcta cgggtagtgt ccagattgtc aacaacaatg cctgggatat
    16141 tgccttttca aatataggac agacggatgc cggaatcttc atcaacgaag ctgcatcttt
    16201 agcagaaaac cctgttaagc tgtttgtggc cctggataat gactggagta cgccaataac
    16261 cgatgcgtcc atcttcattg atgatgttca attgttcaat tcagagaata actggaccat
    16321 tggcgctttc aatgagacca gaaacccgga cgacccgacc gattttggct ggggccaata
    16381 taatccgcaa aaccatcaaa taatcggcaa tgaagtttat gtcgttaaaa aacgcaatgg
    16441 agattttatc aagtttaagg tcgatgctta cagaaacgga tattatcatt tcaggtatgc
    16501 caatcttgac aattccaatg aggtgcaaga ttctgtggca agaaacacca acgggccaaa
    16561 atctctggta cattactcct ttgactccaa aggaatagtc aagatagaca atggttatga
    16621 tttggtgttt accagatata tcacttctct tgaagacact cccggacatt tcatcaatta
    16681 ttcggttacc ggattcttac ttgctcccgg gactttagct gttgttgcag atagtgtaga
    16741 tgtcgcaact gtcaaggagt ctgattattc cttagcatat tcttccgctc cgaccacgat
    16801 tggtcatgat tggaaatctt tcaatctgac ctcaggatgg caaattgatg aagaccgggc
    16861 taattttgtc aaaacaaaaa atggagaaac ctataagctg gtttttgttg attttagcgg
    16921 atcatcatct ggtaccgtta ccgtagaacg gacaaagctg gccaccagca aagtcaaccc
    16981 ggacttacaa ttcccaggca tgacacttta ccccaaccct gcacaaaatg tcctatatat
    17041 tgactcggac aagcctgaac ccgtccgtgt aagtgtaatc aacagccttg gcagagttgt
    17101 tctggaagaa gcaactgata cacgtagctc cataacccta ccttctgata tcacgcccgg
    17161 catgtatcac attgtggtgc aaggtaaaga acgcaggtcc gctttttcaa ttgtaatttc
    17221 aaaataacaa tatagctttc aataaagaga gtctggtttt tagctctctt tattgaaaga
    17281 ctttttccta tcatgaggag tttgtttcca atattcatca tcattgcttt cccgttagtg
    17341 gttacggcac aatcccacac gacggaagcc tttctggata gtattgaaag gagtgttgtg
    17401 ctcgaagaat atgttcatac tgctgaatac aaaccaaccc atgtcaaaaa ggccttgcac
    17461 tccatagaag ttttgaagac agagtccctc aacagaaggg gtgtgaccaa tctcgaagag
    17521 gctctatcga tcattccctc agtaagatat gactatgatc cgattttggg aactcaactc
    17581 cggcttcggg gtatttcagc ggccaatgta gccatcttgg tggatggcgt acctgtgata
    17641 ggacgattgg atggggcgat caacatttcg caaatcccct tgattaacgt caagcaaatt
    17701 gaaatcatcg aagggcctct ttcgactata tatggaaaca atgctgccgg aggtgtcatc
    17761 aacataatca ctactgacag tcagtccaaa aagtggagtt tgggcggaag cagccaaatt
    17821 gaatccattg ggacgcagaa tcatcaattg gatcttgggt tgcagacaaa gaaatggtat
    17881 gtggatatgc acggaaggtt tttcaactat gacaaatacc ccatagacag tcttcgtctc
    17941 ttggaaaaat atacccttcc ggatggctcg ataagtacac gctccaaata tccctggaat
    18001 cccaaactcc aaatgagtgc cggaactact gtcaagtata aggtcagtgc agataattca
    18061 ctggtattta aatatgattt gagcaaggag tctctgctgg actatggacc agtcaaaaga
    18121 cctgccttca aaccatatgc gtttgacgat cgttttttga ccagtcggga agatatctcc
    18181 tttatgtacg atggactcct gtttcataag ttgtatgttg aattgaaatc atcctataac
    18241 aggtttggcc gccatgtgga caatctgttg tttcagtttc aggaaaatca atatgacgag
    18301 acacaaacca aatctgacac gacaagattc agatcttttt tcaacaagct aaacctctcc
    18361 atgaggataa ccccgcaact ggaagtatta ggcggtgtga atcatacttt tgatgccgga
    18421 aaaggagggc ggatcaaggt cgaaaaccct aatgaacatg aagcaattat gtcagaatgg
    18481 gcctattttt cagaagtccg gtattcattg tttaaacgct tcactttttc ttattcgggc
    18541 aggttcctga cccatagtga atttggaaac aattatactt ctggcatcaa ggccaaggca
    18601 gatctgggaa aaaatatcgt cctcagggca tcttatgctc agggatatcg gactccggac
    18661 ctaaaagaac gttatataga gtttattgat gtcaaccata atatcatagg caatccaaac
    18721 cttcaaccgg aaaaatcaaa cgatatccag ctgaccattc aatataatcc ttatcagtgg
    18781 atgaatgcca aactaaatgc atttcgaact gaaatcaatg ataaaatttc actttttcaa
    18841 tatgctcccc taaaatataa ttacagcaac gttgatatct atacggtttc cggtttgaag
    18901 tgggatatga gcggctcatg gaagcagctg aactgggcct taggagcaag tctcggattc
    18961 tggaacacca ttattgagtc ggaggaaaag ccgtataaag gaaaggtact ggactacaat
    19021 gctactttaa actataccat acccaaagta aaaattgccg ccatgctcaa ttacaggtac
    19081 aatggtcctc aaccattatt cagtgtcgat tttggtgatg tcaatatatc agaaataagt
    19141 gctgcgtcat ttttggattt ttctctcaac cgttattttt ggaaagaaag gttgcaatta
    19201 gtccttgggt gtaaaaacat cctgaataca acttatgcct cagtatccgg cgctgacaac
    19261 tcgggtagtc acagagtgtc cggcttccag tttatcaatc cggggcgtaa ctattttgta
    19321 aagcttggca tcaacttgtg aaaccgcaat aagcatcttc ttactaacca cccggataaa
    19381 gatttggcga aagaaactgc ttagcgtgta tccgcagtta caaatttgtt ttcacactaa
    19441 tagtacttgc gtgatttgtt ttcaaaactg tttgggtttt tgtttttgtc cggcaagaat
    19501 agctatttct tcaattcgtt taagtcgggt ttcgggtcgt ttcgccaata caatccaact
    19561 taacatcatc ttttgggacg atttgcttaa accctgaaag tacgcctttg aacctttgtg
    19621 tttcccaaat gcttcgttca aatcggccgg aatgatgagt ttttcaactt catccagaat
    19681 tgtccacgaa ccgttttgct ttgctatttc aatactttca taccccgcga ctgtcattaa
    19741 tccctggtca ataagctgtt gtactttctc tttatttatt cttgaccagg tgcttttctc
    19801 ttttctttta ctaaaaaatt gatgtgatgt ctcgtgatct attttaattt ttttgctgtc
    19861 aatccacccg aagcagagtg caacatcaac agctccgctc caggtaattg attttttatc
    19921 tgatgtccgg gaataaaaaa ctaaccatac agattgttta gacagatggt tctcggtcaa
    19981 ccatcccctc caatctgcaa ggcttttagg gtaaaatacc tcggttgtat tcatgattat
    20041 ttagcttctg ttggctccac cccgaccgtc catgtcgaaa aaagttcttt gaggtcaatc
    20101 aatgttgtgg ctagtttgat accatgcttt cgtacacatg gcacatccgt gtccatagat
    20161 tcccaatggt tgccggaggc tatatccatt ttaccttatc tttttattat ctgcaaatgt
    20221 attcaggatt cttctctggt tgtccatatg tttgagattt taacaacatt cccaaatgta
    20281 aaaggccggt attgggtaga tctctccata cttgatttgt tcttcaatct tcagatacgg
    20341 accaaacata ccagaaagat tttatgtgca ccaatccggc gaataaatat ctgggggata
    20401 atgaataaat accgaatttc atctcatcat ccgaaactcc gcactgactt ccattcgcag
    20461 tgtatcgcca ttccacattc gttgtaagga caataacctg tatcctgtat gtggaatata
    20521 tgtgaggaac tggtcgtggc ggttgatcaa actacgtcgg cgattgtgca ctcggatgtt
    20581 ggacaccgtc cctttttcca tgttgacttc gatgctttgc accggcaaat cttcatcaag
    20641 cgcccggtat tggtatctgt cagcatcgcc ataaacgccg aaaacggtat ctgctttata
    20701 tttgggtatc cagtccggat tgttcaggtt gagtttgtcc ataattatgg tttcaccggc
    20761 atttaagctg tcggtaagca agagcgtatc tgtctgcccg ttgatggtaa tggtcttgaa
    20821 taaagccttt tcgcttcgtt cccttctgta atcatccaag atataatcag acacctgaaa
    20881 gaacgggttg gatgccgcac ctgactgttt tacttccgcc ttgcagctta gaaggagcaa
    20941 cgatacaatc acggcaggcc atcgcaacca gatcagttcg gttagccggt taatcattgt
    21001 accagatttt ggccggtcat aatctccggt tgcttgattc ccatagaaaa caggatggta
    21061 ggagctacat cggccagtct cccttcacct agtgtaaggc cctgtgtatc atttccgaca
    21121 aagatgcatg ggaccggatt cttagtatgg gctgtattgg gacttccatc ctcattgatc
    21181 atgtaatcac tgttaccgtg atcggctatg atgatcatcc tgtaatccag tgcaagggct
    21241 ttttctgtga ttcttcccag acatccgtcc accgtttccg cagccttgac cgcggcctca
    21301 aagacaccgg tgtgccccac catatcggta ttggcaaagt tgacacagat gaagtccggc
    21361 tttttatcat ccatggcgtc caggacagct tgacatatac cctgtgcgct catctccggc
    21421 tgcaggtcgt aggtggcaac cttgggagat ggaatcaatg tgcggaattc tccggaaaac
    21481 ggctcttccc ttccgcctga aaagaaaaag gtgacgtgtg gg
//
LOCUS       DAOQQN010000006        21076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_748_length_21076_cov_5.169783, whole genome shotgun sequence.
ACCESSION   DAOQQN010000006 DAOQQN010000000
VERSION     DAOQQN010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 21076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21076
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_748_length_21076_cov_5.169783"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLG24_00495"
     CDS             <1..262
                     /locus_tag="PLG24_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ23386.1"
                     /translation="IVQEFLQNSLKHSRCKSINIELKAEDSAFILLMEDDGIGFDLHA
                     DNIKGVGLEGIRRRVEFLHGTCDMESNTGEGFRCLVTVPQEK"
     gene            275..940
                     /locus_tag="PLG24_00500"
     CDS             275..940
                     /locus_tag="PLG24_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ23387.1"
                     /translation="MSYKVAIVDDHTLIAKAIAGIVNDFEEFEVLYEVENGKKLIERL
                     ERPGAAPDIVLLDISMPVMDGYQTAAWLSANKPGIKIMALTVNSDEESLIKMVKAGAK
                     GYLQKNIHPMELHEGLRRMINTGFYFPDWATSHLMTSIASKDEANHRNILNDRETEFL
                     RYVCSDLTYKEIAQKMFCSPRTIDGYRDALFEKLHVKSRVALALFAVREGIFDIDHPE
                     SFG"
     gene            complement(929..1270)
                     /gene="gldC"
                     /locus_tag="PLG24_00505"
     CDS             complement(929..1270)
                     /gene="gldC"
                     /locus_tag="PLG24_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HMZ23388.1"
                     /translation="MATEQYTITLKVGLDENKVPEILSWDASDSDNIKNKEIKAFLLS
                     MFDSETRDTLKIDLWTKDFQVMEMDRFFYQTFRAMCDTYQKSTQNNELANAMRDFTNY
                     FGEKTGIILSK"
     gene            complement(1315..2532)
                     /locus_tag="PLG24_00510"
     CDS             complement(1315..2532)
                     /locus_tag="PLG24_00510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMZ23389.1"
                     /translation="MKILQLCKKFPFPLIDGESLAVNYLSRSLAELGCDITLLTMNTS
                     KHYYQLEKTVPAELKHYRQIYHVEVNNDITIKGAFLNLFSSESYHISRYQSKLFVEKL
                     IEILKKEQFDVIHLETLYLTQFIDVIRKYSNALVVLRSHNVEFEIWERLTENQMSQVK
                     KFYLQYLTRKLKRFEIRKLQEIDLLLAITQRDLKTFRSYGYLKAAKVVPIGLDTSDYL
                     AEEKDVFSNPSMSFIGSLDWIPNMEGLDWFLNDIWPRISSKYPELTFHVAGRNMSTNI
                     SKRKIPNVFFHGEVSDARRFINAHPITVVPLLSGSGMRVKILEGMFLGRIVVTTSLGL
                     EGIDAQHKNQVLIAETADEFMKAIDFCFEHPVQTQNISRRAQVFAARNYDNLEIGKEL
                     LSFYRMKKESVPT"
     gene            complement(2633..3256)
                     /locus_tag="PLG24_00515"
     CDS             complement(2633..3256)
                     /locus_tag="PLG24_00515"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HMZ23390.1"
                     /translation="MLIEINPLNPDSRAINSVVEKMKNGAVIIYPTDSVYALGCDPES
                     KKGVEKLYQLKNSDPSKSPLTFICDTISMASAYSSQISNSNFRFIKDHTPGPFTFILP
                     ASNNLPKILHVKNKTYGFRIPDHRIPQELIKALGRPILSASLKSIEREDEDQFYMYPE
                     DIYSDYSLLVDLVIDGGPGSLEQSTVVDLTDGTPVVIREGKGNVETE"
     gene            complement(3341..4819)
                     /locus_tag="PLG24_00520"
     CDS             complement(3341..4819)
                     /locus_tag="PLG24_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HMZ23391.1"
                     /translation="MKEINTLGALKASGYTYKTVKDELRDNLILKLQNKEKVFEGIFG
                     FDDTVLPDLERAILSRHNILLLGLRGQAKTRIARLSVNLLDEYIPIIKGSELNEDPLH
                     PITAASKELVASMGDETPIEWLHKSNRFLEKLATPDVSIADLIGDIDPIKAANNRWSY
                     ADERSIHYGLVPRSNRSIFVLNELPDLQARIQVSLLNILQEGDIQIRGYKMRLPLDIQ
                     FIFTANPEDYTNRGNIITPLKDRIDSQILTHYPDQLSVAKQITLQEARIGKEVVEKVF
                     LSSPAHNILERIAFVARDSEFVDEHSGVSARLSISGLENLYSAAYRRMLRGDYDSTLI
                     RVSDFIGVIPSITGKVELVYEGEQEGPYNVALNLLGGAIRDEFLKVFPDPEKNIKKAE
                     NDPYIAIKAWFAKGNTVEFWYEDSDDEMFDKLMEVDGLHNIVADKANNRAEVIILMEV
                     VLHGLAELNMITREMLDDKISFNDSLADMFDDLLNTDNEDDY"
     gene            complement(4928..5542)
                     /locus_tag="PLG24_00525"
     CDS             complement(4928..5542)
                     /locus_tag="PLG24_00525"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HMZ23392.1"
                     /translation="MNKTVWVIRHGETELNASGIVQGQGMDAPLNIKGFEQAESFWKV
                     YSEEPFDLVLYSNLIRSQQSVQRFLTSGVQGVELADLREISWGVNEGKQPTPDVLQRY
                     HAVIDAWQSGDFAAAVEGGESAIQLAKRAQTCITYFNEINRKNILVCTHGRTIRVLAC
                     LMLNRPLTAMEDFHHANLGLYRFDMDGDGWQCTLYNDIRHLQGL"
     gene            complement(5890..6531)
                     /locus_tag="PLG24_00530"
     CDS             complement(5890..6531)
                     /locus_tag="PLG24_00530"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HMZ23393.1"
                     /translation="MLIFLTSGFSQYVRMGVSSVKASSPEAQQQIEATMKSMKIESFA
                     TPDKVRSDVNMMGGMVVMSTFTNPNDDSFVMYMDMMGQKMKVVPTPEELKSIKEEGDN
                     NSKDMKITEVPGDSKTILGFKCKKINVSNGDMNVVMYVTPDLKMKANRIQGMEGLKID
                     GYPLEYTVEAAGTKMTFTADKYEPTFDDGKMKEPSGKYKEMTFTEFKKQMPGM"
     gene            complement(6688..7233)
                     /locus_tag="PLG24_00535"
     CDS             complement(6688..7233)
                     /locus_tag="PLG24_00535"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HMZ23394.1"
                     /translation="MHYILFDLEATCWLGRPPIGNNEIIEIGAYKVNGYGEIVDHFNQ
                     YIKPISNPTLSGFCKKLTGIQQHNVDTAFKFDKVIGDFMEWFGYEDNDEYHLISWGEN
                     DRQLLFQDCTLHDVEDDWIRFWVNLKPAYQRLTGMLKQVGLKKALERETIEFEGKQHR
                     AITDAYNLTKLFIKHFDDWEL"
     gene            complement(7234..8973)
                     /locus_tag="PLG24_00540"
     CDS             complement(7234..8973)
                     /locus_tag="PLG24_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HMZ23395.1"
                     /translation="MNRLSESRPLEDPISREKDQIYPPLIDDINQWPINKLTENRSAF
                     VKKLEAYSFNTISRKSTDFIKDLIQKTIFQEKTRIKEEPWRIDPPNDRVFWNRIRKKM
                     ADSDIDSSHQKEENVNESLKLIIHRYAEEIVGTFNTKTYRLIRKILTILFKRLLNKGI
                     KVAPSNKVDKVLLDKLKIYGPIEKIRMLSSKGTVVTLPTHSSNLDSVLIGYTMDYKLG
                     LTGSAYGAGLNLYNFGPAAYFMNRLGAYRVDRRKKSVIYLTTLAGMSKLAIQAGTPSL
                     FFPGGTRSRSGGLEDKLKLGLLNSVIEAQRSIYEEGRNDKVYVVPLVLSYHFVLEAKN
                     LIEDFLKKTGKERYVKSRNEFTSFRSWTKFLYQFISKSTTISLSFGEPMDCFGNFLND
                     EGESVDPRGKVIDTRDYFMYEGKLTSNHQRESEYTKNLGNIVAERYKAENIILSSHLV
                     SYVAFQLLKAANPNLDLYSLLSLPEDEFFFSREQFLEQSGIVVEMLKGKALERLLKLP
                     EIFDRDILTLVLDGIDQIGTFHSSRPITIHKNGDFYSQDFRLLYFYNNRLESYGYESE
                     IAWNNLPVVYQID"
     gene            complement(8951..9559)
                     /gene="yihA"
                     /locus_tag="PLG24_00545"
     CDS             complement(8951..9559)
                     /gene="yihA"
                     /locus_tag="PLG24_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HMZ23396.1"
                     /translation="MAGQLNIHFKGSFSDVKACPVTTLPEYAFIGRSNVGKSSLVNML
                     SGKTHIAKVSNTPGKTQLINLFEINEQWNIADLPGYGYAKTSRVNRTAWGKMVTNYLL
                     QRENLVLLFVLIDSRLPAQKIDLDFMEFCGKKQIPFSIIYTKSDKSSTNQLKKQIAEI
                     QKELLRHWNALPDQFISSSQNKSGRDEILDYIYGINEQIVRK"
     gene            10004..14638
                     /locus_tag="PLG24_00550"
     CDS             10004..14638
                     /locus_tag="PLG24_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ23397.1"
                     /translation="MKNTTTLGLNRGPSHRLLNTRMSRGATFLAIFIMVLYASGIGYS
                     QYYNVNQNCTTSFDDISGTGTQLTVNGDGHTFNSGLPFNFQFFGVNYTTPSIKISMDG
                     YIMWNTTTGDLVNDNLPLPVNDPDINTYGAIYPFWDFSFTDISTPPAGVYVETKGIAP
                     NRVFMVQWEGVQRVIDETTPTYSYGKVTYQVLFYETTNEIKFVYEDTFYGSTIADGSE
                     NNNGASSTIGLQKGGSPIRIYQTSFDDPTLLQGSSCISYLPAATTSGCTMACNTTNIG
                     IPANCRPTLIARDFLTTSYDCATSFIIQLLQTPTGPILEQGVDSLVADGISIGGNPYP
                     LVNQNYVINIIENITGGSPNSCWNWHTFQDNVAPEIVCRPADTVFCYEPLVLYSLTGI
                     TDCSGPLTAHVIDSTYTPYSCSIDPYLLGRITRRYFLTDASGNRSDTCEDVLFLAPPS
                     KDSIQFPPHFGTLQCDDNFAADAYGRPLPSVTGVPTIRNTSINLYPSTLGLLCNMTTS
                     YSDHELPMGCGIKIMRRWVSYFWTCAGEDSIAHIQEIIISDTVPPVVTAPADVTITAG
                     QNCRASYTVPPATITDNCNHLGNVSVIHPNGVTNQNGGFTITNLSIGTYNIIYSVNDG
                     CGHIVRDTTKLTVQDLTAPVAICKEAIVSLDNTGTGRMFASSINNGSHDNGCGPVTLK
                     VRRMNPDCNGAPDQTTWADYIDFYCCDLPNNPIQVILQVTDAGGLTNTCMTTVTVQNK
                     NTPIIAYPLPNIIVPCGTSFDVNNLALTFGEYVTNPADRQKIIIDGVDKGWDGLITGV
                     CTATVTETAPTINIPQCGFGTITRHFLITDGGSFQQTVSQTITINYDGVELTTADFNA
                     PLDYVVTSGICTPPDLANADLGSPYVPTLKSPLNGCHNIMINKEDIVFTNASNACFKI
                     VRTWTIIDWCLAAAKGNQYALDHAVKFTQALVVMNTTAPTFVPHADQIVETTNCTASN
                     VTVSTSATDDCPSNFLTYSWQLDLNFNNNTNTWDHQGTGSSFTINMPIGKHRVHFAVT
                     DGCGNVEHQYFTVEVKNVKQPTPVYTNLTVDLMANLQVTIPARLFNRASFGACPNSYP
                     LRFAYSSDPADSLRTFDCSFGVDTAHPVTIYVIDANGLFDFAITNLTLNDNIVQCPNT
                     ITVISGQIVTEGNAGIPQVEVNAHSSMSRLTDGNGQFRFNNVHPGETYYIEPKDDNNP
                     LNGVTTGDIIKIQNHILGKTSLPTPYKIIAADVNEDKSVSISDIVMIRKMILGKIDQF
                     SSGKSWKFIGKNYTFSNPAAPLKESYPQSIPAATNSYLDGIDFIGVKMGDVNGDFTLN
                     FDDPKVDSRSYAKLSIEDVRMKAGEITTVHVVNASQINVQGLQANLSFAKGVKLVGIE
                     SQMMEAGDDNFYQFSNTLKLSLTSKDVLNDNLIITLYLVADNDMDVSDAFQLNHTGFA
                     SELYDNEGNGNPIELTYTNSQSNLQVEQNIPNPFSDQTQISFTLGSADHVTMKIYDTN
                     GRVLKYINAFYAKGRNTITIDNGSINASGVMFYEITSNRKTVTKKMVKLK"
     gene            14892..15818
                     /locus_tag="PLG24_00555"
     CDS             14892..15818
                     /locus_tag="PLG24_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HMZ23398.1"
                     /translation="MEKQYTNNIMMIRPANFMYNEQTAVTNAFQTNDEGGRASNVNVL
                     ALQEFDNFVLKLKEAGVNVIVMHDSLSPIKPDAIFPNNWVSFHGNGTVITYPMATVNR
                     RIERREDIIDALGLVFKVNSRYSFESSEEDNQFLEGTGSMILDRENKIVYACLSPRTD
                     IFLLDRFCLLMGYGLVSFYSTDREGKEIYHTNVMMAIADQYAVVCLDSIKDEDKRMEV
                     RDSLEKTGKEVIELTFDQVLHFAGNMLQVEGHPGKRILVMSQQAYDSLTREQIDKINS
                     YNPILAVPIPTIEKYGGGSVRCMMAEVFLPKR"
     gene            15857..17191
                     /locus_tag="PLG24_00560"
     CDS             15857..17191
                     /locus_tag="PLG24_00560"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HMZ23399.1"
                     /translation="MSISAMKKATLSGESYKIMIEENEIPTHWYNILADMKVQPNPTL
                     QPENRQPVTRDYYADLYPQGCIEQEFSTERWIEIPEEVRNIYKIWRPTPLVRAHRLEQ
                     ALQTDCRIYFKNEGVSPAGSHKTNSAIPQIYYAARDGVKKVVTETGAGQWGSAVAFAA
                     QMYNLDCEVFMVRGPYNQKPSRRQMMQLWGAHVTASPSELTAVGRNQLAENPNSKGSL
                     GTAISESIEYASQDKDIQYVIGSVQNFVLLHQTVIGQECLKQFEKIDEYPDVVVAPLG
                     GGANFAGICFPIMRDLILGKTKTRFVASEPLTCPKLSEGEFRYDYGDVGKKTPLFAMY
                     TLGHDFMPVPNHLVGLRYHGAGPIISQLAKEGHIDAMSHDQKECLQAGILFAKTEGLI
                     PAPESTHAIASVIREVNRAKQEGKSKTILFNLCGHGLLDLFAYDDYLNGKVS"
     gene            17611..18156
                     /locus_tag="PLG24_00565"
     CDS             17611..18156
                     /locus_tag="PLG24_00565"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HMZ23400.1"
                     /translation="MRIYTKKGDKGSTSLFGGKRLAKDAVRIEAYGTVDELNAQVGVL
                     LDNLENADIQDDLLRIQHWLFDYGSMLATPPGAKLYVTPPGEKALTFLEEKIDAMELE
                     LPPLKHFILPSGHTSTSFAHMARCVCRRAERRIVSLSHEEEIGEEPLAFFNRLSDYFF
                     VLARYLTHLAGKKDVEWNPRD"
     gene            18210..20300
                     /locus_tag="PLG24_00570"
     CDS             18210..20300
                     /locus_tag="PLG24_00570"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HMZ23401.1"
                     /translation="MEQKINFSILRNLTASYRILIILTSALIISLLLPRVKTVNYEFE
                     EGSKWKYSDLRAPYDFAIRKTENEIAAEKEAIVKNFIPYYKKNESTTENLNDRILEEY
                     NTYQSHAGGDTALFLSNYKDINIKQIRSVFQKTYRTGILEANDIYKDREKEFLIHIVE
                     GNIISLKPRAAIPTVVQAKNELHRDIRQAGEHGPLLYPVIDALIEPNIFLDQTLSNEF
                     LNQAFKNIATNEGIVRKGDIIINRNSTISKDTYQKLISLRELDDAGNTGKLSYFKSFL
                     AYFVFISVILMFYVLYVRTYVREVFLIFKKMLFMHLWLILYVFMTYFVIKSSSLDLLM
                     IPFCIAPIVIKNFYNQRLAFFTLVAILTIVALFGPVTKDFIILQITAGIMVVIVTKET
                     RYWSKFFLLLLFLLGTYIFTYLFLTYIKNNNLADFNLNSIWWICLNVFLTLLAYPLIP
                     LLERLFGFTSGITLAELSDLNNNLLKELSIKAPGTFQHVLHVATLAEAAAEKIGANSS
                     MARVGALYHDIGKMKAPYNFTENQTNINPHDSIDHVTSAKLIIEHVPEGVKIAKKNGL
                     PNVIIDFIKTHHGTTKVEYFYRKFVKEHADHAVDEKVFTYPGPRPRTKEQVVVMMADT
                     LEAAVKSLINPTEEEINDMVDKLIRAKIEDGQFDRSDITFEDVQTCIRIFKNMLVNMS
                     HIRIAYPDPRPETN"
     gene            complement(20270..>21076)
                     /locus_tag="PLG24_00575"
     CDS             complement(20270..>21076)
                     /locus_tag="PLG24_00575"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HMZ23402.1"
                     /translation="KPIPTPIPHIRYGMFLSFTFLGGLFAFLSKTYPVRWQVFVLLTT
                     LYLGFSILFLSVRTAWLVSFLGLLIILAYFIMQKRRITLISIVIIAAIGGSILAYLFI
                     PSVKIKAGYTLYDWRKFREDKGSEYSDSERLYSLQNGWSLWKENKWLGVGSGDLHRQL
                     EQNAIKNHYPGSQIPHNQWLMTLVCGGLLSLAFTLLFWYQLWRNPIHRKLFLFHLLVI
                     LYLVTFFFEPTFETSIGVLSFVFILIMILLQSYQGEQKSRLQLVSGRGSG"
BASE COUNT         6302 a         4531 c         4179 g         6064 t
ORIGIN      
        1 aattgtacag gaatttctcc aaaacagcct gaaacattcc cggtgtaaaa gtatcaacat
       61 tgaactaaaa gctgaggatt cggcatttat attattaatg gaagatgatg gaatcggatt
      121 cgacttacac gcagataata taaagggagt aggtctggaa ggaatccgca gaagagtaga
      181 attcctgcat ggtacatgtg acatggaaag caacacggga gaaggtttca gatgtcttgt
      241 gactgtgccg caagaaaaat aaaaatttgg ggtcatgtct tataaagtag ccatagtaga
      301 tgatcataca ttgattgcga aagccatagc aggtatcgtc aacgattttg aagaatttga
      361 agtcctttat gaggttgaaa acggcaaaaa attgatcgaa cggttggaaa ggccgggagc
      421 agcaccggat atcgttttac ttgacatatc gatgcctgtg atggatggct atcaaactgc
      481 tgcctggctc agtgcaaaca aacccggaat aaaaatcatg gcattaacgg tcaacagcga
      541 tgaagagtcg ttgattaaaa tggtcaaagc aggggctaaa ggatatcttc aaaaaaacat
      601 ccatccaatg gagttgcatg aaggcctaag gcgaatgatt aatacaggat tctactttcc
      661 ggattgggcg acttcccatc taatgacgag catcgcatcc aaagacgagg ccaatcatcg
      721 caatattctg aatgatagag agacagaatt cctgcgttat gtatgttccg atctaaccta
      781 caaggagatt gcgcaaaaga tgttttgcag cccaaggacg atagacggct atcgggatgc
      841 attattcgaa aaactgcatg tcaaatccag ggtcgctctg gctctatttg ccgtaagaga
      901 aggaatcttt gacatagacc atcctgaatc atttggatag tatgatacca gtcttttctc
      961 cgaaatagtt cgtaaaatca cgcatcgcat ttgccaattc attattctgc gtggatttct
     1021 gataggtatc gcacatggcc ctgaaagttt gatagaaaaa tctgtccatc tccatcacct
     1081 gaaaatcctt ggtccaaaga tctattttca aggtatccct tgtttcagaa tcaaacatgg
     1141 acagtaaaaa tgccttgatc tccttatttt ttatattatc tgaatccgat gcgtcccagg
     1201 aaaggatttc aggcactttg ttttcatcaa gtcccacctt cagggtgata gtgtattgct
     1261 ctgtcgccat taatcatttg tttgaatggc cacaaaagta attaaattat aaaactatgt
     1321 cggaactgat tccttcttca ttctgtaaaa ggataatagc tcctttccaa tctccagatt
     1381 atcataattt cgggcggcaa atacctgagc cctgcggctg atattctgag tctgaaccgg
     1441 atgttcaaaa cagaaatcta tcgctttcat aaactcatcg gcagtttcag caatgaggac
     1501 ctggtttttg tgttgtgcat cgataccttc caatcccaac gaagtggtaa cgacaatccg
     1561 tcccaaaaac attccttcca gaatctttac tctcatgccg ctgcctgaaa gtagtggtac
     1621 gacggtaatt gggtgagcat tgataaatct cctggcatca ctgacctcgc catgaaaaaa
     1681 aacattggga atctttcttt ttgatatatt ggttgacata ttcctgcctg caacatgaaa
     1741 ggtcagttcc ggatattttg aagatatgcg tggccaaatg tcattaagga accagtcaag
     1801 cccttccata ttgggtatcc aatcaaggga tccaataaag ctcatcgatg gattactaaa
     1861 tacgtctttt tcttcagcga gataatctga ggtatcgaga ccgattggca cgaccttagc
     1921 tgcttttaaa tatccgtatg accggaaggt cttcaaatca cgttgtgtta tggcaagcaa
     1981 aaggtcgatt tcctgtaatt ttcggatctc aaagcgtttt aattttctgg tcagatactg
     2041 aagatagaac ttcttgacct gactcatctg attttctgtg agacgctccc agatctcaaa
     2101 ttctacattg tgggatctta gcacgacaag cgcattacta tactttctga ttacatcaat
     2161 aaactgcgtc aaatacaatg tctcaagatg tatgacatca aactgctctt tctttaaaat
     2221 ttcaatcaac ttttcgacaa acagtttact ttgatatcgg ctgatgtgat aagactctga
     2281 cgagaaaagg tttaaaaatg cccctttgat tgtaatatca ttattgacct ccacatggta
     2341 aatctgccta taatgcttca actcggccgg taccgttttt tccagttgat agtagtgctt
     2401 ggaggtattc atggtcagta aggtgatatc acaacccagc tcagcaagac tcctactcag
     2461 gtagttcaca gccagagatt ctccgtcgat caatggaaaa ggaaattttt tacagagttg
     2521 taatatcttc ataccaaatt ttcgggatgc aaattaagaa taatctaatt atccttgatg
     2581 taacgcaaca ggaaatatct aaattgtcga aagttaaatt tttatataca acttattctg
     2641 tttcgacatt tccttttcct tcccgtatta caaccggagt accatcggta agatccacca
     2701 ccgtactttg ctccagagaa cccggacctc cgtcgataac aaggtccacc aacagtgaat
     2761 aatcggaata aatatcttcc ggatacatat agaactgatc ctcgtcttcc ctttctatgg
     2821 acttcaaaga tgctgacaat ataggccggc caagggcttt aatcagttcc tgcggtatcc
     2881 tgtgatcggg tatcctgaat ccgtaggtct tgttcttcac atgaagtatt ttaggcagat
     2941 tgttagaagc aggcagaatg aatgtaaaag gtccgggagt atgatccttg ataaaccgga
     3001 aattggaatt ggatatctgg ctagaatagg cagaggccat ggaaatggta tcacaaataa
     3061 atgtcagagg gcttttggat ggatcggaat ttttcaactg ataaagtttt tccacacctt
     3121 tcttgctttc cgggtcacag cccagcgcat aaactgaatc tgtcgggtaa atgatgacag
     3181 ctccgttctt cattttttca acaacgctat ttattgccct tgaatcaggg ttcagtggat
     3241 ttatttcaat caacatgtca tttatttttt atatatacat taatattttt ctaaaataaa
     3301 accggctaag aagtcaattc aattcttcat ttgtcagcta ttaataatca tcctcattat
     3361 ccgtattaag gaggtcgtca aacatatctg ccaatgagtc gttgaaactg atcttgtcat
     3421 ccagcatttc ccgggttatc atgttcaatt cggccagtcc atgcaagacg acttccatga
     3481 gtatgattac ctccgcacga ttatttgcct tatctgctac gatattatgt aagccatcca
     3541 cttccatcag cttatcaaac atctcatcgt cactgtcttc ataccagaat tcaactgtat
     3601 tccctttggc aaaccaggct ttgatggcaa tatatggatc gttctctgct tttttaatat
     3661 ttttttcagg atcggggaag accttgagaa actcatcccg aatggctcct cccaacaaat
     3721 tcaaagcgac attatacgga ccctcctgtt caccctcata caccagttca acctttccgg
     3781 taatgctcgg aatgacgccg atgaaatcag acacccgaat cagagtcgag tcataatctc
     3841 ctctcaacat tcgccggtat gcagcactgt aaagattctc cagtcctgaa atactcaatc
     3901 tggcagacac tccactatgt tcatcaacaa actcactatc acgggcgaca aaagctattc
     3961 tctccaaaat attatgtgcc ggtgaggata aaaacacctt ctcaacaacc tccttcccta
     4021 tcctggcctc ctgtaaggta atctgtttag ctacactcag ctgatccgga tagtgtgtta
     4081 atatctggct gtctatcctg tctttgagtg gcgtgataat attgccccga ttggtataat
     4141 cttccggatt agcggtgaaa ataaattgaa tgtcaagcgg caaacgcatc ttgtaccctc
     4201 tgatttgaat atcaccttcc tgtaggatgt tcaacaggga cacttgtatt ctagcctgca
     4261 gatcaggcag ctcattgagt acaaatatgc tacggttgga ccgtggcaca agtccataat
     4321 ggatagacct ttcatccgca tagctccatc tgttgttggc agctttgatg ggatctatat
     4381 cgcctatcag atcggcaatg cttacatccg gagtagccaa tttttccaaa aaccgattgc
     4441 ttttgtgcag ccattcaatg ggtgtctcat ctcccataga agcaaccaat tcctttgagg
     4501 ccgcagtaat gggatgaagt ggatcttcat tcagttcact tcctttaatg ataggaatgt
     4561 attcgtccag aagattgaca gagagtcttg caatccttgt cttggcttgt cccctcagtc
     4621 ccaataagag gatattgtgc cgggaaagaa tggcacgctc aagatcaggg agcaccgtat
     4681 catcgaaacc gaaaatacct tcaaaaacct tctctttatt ttgcaatttc aagatcaggt
     4741 tgtccctcaa ttcatccttt actgttttat aagtataacc ggaagccttt aatgctccca
     4801 gtgtgtttat ttctttcata atcaaaagat tgtgttttga cgcattgctc tttttccgat
     4861 ctttatccat gatcctaatc ttgctgagac catgaaacca atcccttccg acgattcccc
     4921 gggcttacta cagaccttgt aaatgcctga tatcattgta caatgtacat tgccaaccat
     4981 caccatccat atcgaatcta taaaggccca ggtttgcatg atgaaaatcc tccatagcag
     5041 tcaaaggtct attcagcatt aaacaggcta atactctgat tgttcttcca tgagtacaaa
     5101 ccaggatatt ttttctgttg atttcgttga aataggtgat acatgtttgt gctcttttgg
     5161 ccagttgaat agccgattcg ccaccttcca cggcagcagc gaaatcacct gactgccagg
     5221 catctatgac agcatgataa cgttgtaaga cgtcaggtgt cggttgctta ccttcattca
     5281 ccccccagct tatctccctc aaatcagcca gttccacacc ctgcacacca gaggtcagaa
     5341 atcgctgtac ggactgttga gaccgaataa gattggaata gagtaccaga tcaaatggct
     5401 cttccgaata tactttccaa aagctttcgg cctgttcaaa accttttata ttcaaaggtg
     5461 catccattcc ttgcccctga actattccgc tggcgttcag ctctgtttct ccatgtcgga
     5521 ttacccaaac tgttttattc aagataattt ccctgttttt gctcctaaat taagtatttt
     5581 taacagaact ctgaaaaaaa taagtgattg gtgatctaaa aatgagcaag tttaaaataa
     5641 ttatgactct caggcattat gactctattg agcgcaattc taagaaatcc accgtggtta
     5701 atagccccgt ttgatcttat cccatcacaa ataaaccatc gcacttataa ccggtcatcc
     5761 cagaaaatgc tctttaaggc actgagctgt ccgacaaata aaattagcaa atgttatagc
     5821 tatcacgact ctgaaaccct accggtcata ataaaaaaag cggccttcaa gccgctttcg
     5881 tgttatgttt tacatgcccg gcatttgttt tttgaactct gtaaaggtca tttccttata
     5941 ttttccggaa ggctccttca ttttgccatc atcaaaagtc ggttcatatt tatctgctgt
     6001 aaaagtcatt tttgtccctg cagcttcaac cgtatattcc aatgggtaac catcgatctt
     6061 cagaccttcc attccctgga ttctattggc cttcatcttt aagtcaggtg tcacatacat
     6121 gacaacattc atatcaccgt tgcttacgtt gatcttctta catttgaatc caaggatggt
     6181 ctttgaatct ccgggaactt ccgtgatttt catatcctta ctgttattat ctccttcctc
     6241 cttgatactc ttgagttctt caggcgtagg cacaaccttc atcttctggc ccatcatatc
     6301 catatacatt acaaaactgt cgtcattggg attggtaaaa gtgctcatga caaccatgcc
     6361 tcccatcata ttgacatcag atcttacttt atcaggagtt gcaaaacttt ctattttcat
     6421 tgatttcatt gtcgcttcta tctgttgctg agcttcgggg ctggaagcct taacagagct
     6481 gacacccatt ctgacatatt gactgaatcc acttgtcagg aatatcagca taaagcaaac
     6541 aattaaatta atttttttca tatttattac cttttgattt ttaaataagt ggttattaat
     6601 tttgttgatt tttagaaaaa tattcatcga atcccgatgc ttaatccaaa taagaaacgg
     6661 attttagtca aaagcaaata tatagcatta tagttcccaa tcatcaaaat gcttgataaa
     6721 aagttttgtt aaattataag catcggtaat ggctctatgt tgtttccctt caaattcaat
     6781 ggtctcgcgt tcgagtgctt tttttagtcc aacttgtttg agcattcccg tcaggcgttg
     6841 atatgcaggt ttgagattga cccaaaacct tatccaatcg tcctcgacat catgcagggt
     6901 gcaatcctga aataaaagtt gcctgtcatt ctcaccccag gatatcaaat gatattcatc
     6961 attgtcctca taaccaaacc attccataaa atctccgata actttgtcaa acttaaatgc
     7021 ggtatccaca ttatgctgct ggatacctgt tagtttcttg caaaatccgg ataaagtagg
     7081 atttgagatt ggtttgatat actgattgaa gtgatcgaca atctcaccat agccattgac
     7141 tttataagct ccgatttcaa tgatttcatt gttgccgatt ggtggccttc ccagccagca
     7201 tgtcgcttca agatcgaaca gtatgtaatg catttagtca atttggtaca ccaccggcag
     7261 attgttccag gcaatctccg actcatatcc gtatgattca aggcggttat tataaaaata
     7321 aagcaatcgg aaatcctgac tgtagaaatc tccatttttg tgtatcgtga tagggcgact
     7381 cgaatgaaag gtgccaatct ggtctatacc atcaagcacc aaagtcagta tatcccggtc
     7441 aaaaatttcc ggcaacttca acagtctttc gagtgcctta cccttgagca tttcgactac
     7501 gatacccgat tgttccagga attgttctct ggagaagaaa aactcatctt ccggcaaaga
     7561 gagcagactg tacaagtcaa gatttggatt ggcagccttc agcaattgga aggcgacgta
     7621 ggatacaagg tgactggaca gaataatatt ttctgcctta tacctttctg ccacaatatt
     7681 tcccaggttt ttagtatatt ccgattccct ttgatggttg gacgtcaact taccctcgta
     7741 cataaaataa tcccttgtat ctatgacctt acctcgtggg tccacacttt ctccttcatc
     7801 attgaggaaa ttaccgaagc aatccattgg ctcaccaaat gacagggaga ttgtggtact
     7861 ttttgagata aactgataca ggaacttagt ccagctgcga aaggaagtga attcattacg
     7921 cgacttaaca taacgctctt tccctgtctt cttaagaaaa tcctcgatga ggtttttggc
     7981 ttctaaaaca aagtgataag aaagtaccaa gggcacaaca tacactttat cattcctgcc
     8041 ctcttcgtag atggagcgct gggcttcaat aaccgagttg agcaaaccca gtttcaactt
     8101 atcttcaagt ccgcctgagc gggatcgggt gccacctgga aagaataaag atggcgtacc
     8161 tgcctggata gccaatttag acatcccagc taaggtggtt agatagatga cacttttctt
     8221 tcttcgatcg acacgatagg caccgagtct gttcataaag taggcagccg ggccaaaatt
     8281 ataaaggttg agtccggcac catatgcgga acctgtcaaa ccgagcttgt agtccattgt
     8341 ataaccaatg agaacagaat ccaggttgct cgaatgtgta ggaagggtca caactgtgcc
     8401 cttcgaggaa agcattctga tcttttcaat gggtccgtat attttgagtt tatcgagcag
     8461 aaccttatct accttattgg aaggggcgac cttgattcct ttgttgagta atcttttgaa
     8521 aaggatggtc agaatcttcc tgatcagtct gtaagtcttg gtattgaatg tgcctacgat
     8581 ttcttctgca taacgatgta tgataagttt caggctctca ttgacatttt cttctttctg
     8641 atgactggaa tcgatatccg aatcggccat ttttttgcgg attcgattcc agaatacccg
     8701 gtcattggga ggatcaattc gccagggttc ttccttgatt ctggttttct cctggaaaat
     8761 tgttttttgg atgaggtctt tgatgaagtc tgtcgatttt cgacttatag tattaaagga
     8821 gtatgcctcc agctttttta caaaagcact acggttttca gtcagtttat tgatcggcca
     8881 ttgattgata tcgtctatca acggaggata aatctggtcc ttctctctgg agatgggatc
     8941 ttcaagcggt ctactttctg acaatctgtt cattgatacc ataaatatag tcaagaatct
     9001 catcgcggcc ggatttattt tggctggagg agataaactg atcgggcaat gcattccaat
     9061 gacgcaataa ttctttctga atttcagcaa tttgtttttt caactgattg gtggaagact
     9121 tatcactttt ggtatagata atcgaaaacg gaatctgttt ttttccgcaa aattccataa
     9181 agtcaagatc aattttttgc gcaggcaatc gactgtcgat cagaacaaat agcagaacga
     9241 ggttttctct ttgcagcaaa tagttagtca ccatcttgcc ccatgcagtc ctgttgaccc
     9301 gggatgtctt tgcataaccg tatccgggca agtctgcaat attccactgc tcgttgattt
     9361 caaacagatt aatgagttgg gtttttccag gagtatttga cacttttgct atatgggtct
     9421 ttcctgacaa catattgacc agagaagatt ttccgacgtt ggaccttcct atgaatgcat
     9481 actccggcaa agttgttaca gggcatgctt taacatcaga aaaactccct ttaaaatgaa
     9541 tattaagttg tccggccaag atatttggat tttcagggca caaagataat aaaatcaaac
     9601 tatcattgct aaaggagtgg ttgatggtgt tccaatctaa gaattgagga ttgttactat
     9661 gaaatatttt attagttaaa aactaaagac caccaatctt taaaaaataa aaattgtcag
     9721 ccgcagtaaa ggaagataga tcaatcaaat ataaagaagt tgattttcaa gctattaaat
     9781 tacacatatc tgatatttat atatatctat aaataaatta tttatatttt acaaaaatat
     9841 ttaatataaa aacacattag aaaatatctt aatgtgtatc tttgtcattc aaatggcatt
     9901 tcaagcagat tggaatgcca cttctcccca ataactgtat aaagaaatgg acgcgctgtg
     9961 catggcggtc cgggtttata ttttctaacc atattttagt cttatgaaga acactacaac
    10021 tttaggcctc aaccgaggac cttcccaccg actactcaat acaaggatga gtcgcggtgc
    10081 aacatttctt gccatattca ttatggtgct atatgcgtcc ggcatcggat acagtcaata
    10141 ctacaatgtc aatcagaact gtacgacatc atttgacgat atttccggaa caggcacgca
    10201 actcactgtc aatggagatg gtcatacttt caacagcggc ttaccattca atttccagtt
    10261 ctttggtgtg aattacacga cgccttccat caaaataagt atggacgggt atatcatgtg
    10321 gaacacgacc acaggagatc tggtcaatga caaccttccc ctacctgtca atgatccgga
    10381 tatcaacact tatggcgcta tttatccatt ttgggacttt agctttaccg acatatcgac
    10441 acctccggca ggagtctatg tagagacaaa agggatagcg cccaatcgtg tatttatggt
    10501 acaatgggaa ggtgtacaaa gagtgataga tgaaaccact cccacttaca gttacgggaa
    10561 agtaacctat caggtgttgt tctatgagac caccaatgag attaaatttg tctatgaaga
    10621 cactttctat ggaagtacga tagcagatgg tagtgaaaac aacaacggtg caagttctac
    10681 catcggacta caaaaaggtg gttcaccaat aagaatatac cagacatcat ttgatgatcc
    10741 tacattgcta cagggctcat cctgtatttc ctacctgcct gctgcaacaa cttcagggtg
    10801 tacaatggca tgtaatacta ctaacatagg catcccggcc aattgcagac caacgctgat
    10861 agcccgggat ttcttaacaa cctcatacga ttgtgcaacg tcattcatta tacaattatt
    10921 acaaacgccc accggaccaa tccttgaaca aggtgtagac tcactggttg ctgacggaat
    10981 atccataggg ggaaacccat atccgttggt caatcaaaat tacgtcataa atattataga
    11041 aaatattacc ggaggatctc caaattcttg ttggaactgg catacattcc aagacaacgt
    11101 agctccggaa attgtttgca gaccggcaga taccgtattc tgttatgagc cacttgtatt
    11161 gtactcactt accggaatta cggattgttc cgggccgctg acagcacatg tcattgattc
    11221 gacctatact ccctactcct gcagtattga tccttatctg ctcgggagga taaccagaag
    11281 atatttcctg acagatgcat caggtaacag gtctgataca tgtgaggatg tcttgtttct
    11341 ggcacctcca agtaaagaca gtattcagtt tcctcctcat tttggaactt tacaatgtga
    11401 tgacaatttc gcagcggatg cttatggacg tcctttacca agtgttacgg gagttccgac
    11461 aataagaaat acaagtatca atctctatcc cagtactctg gggctattgt gcaacatgac
    11521 gacatcatat tcggatcatg aactacccat gggttgtggt ataaagatca tgagaaggtg
    11581 ggtttcatac ttctggacat gtgcaggaga agattctatc gctcatattc aggaaatcat
    11641 tatcagtgat acggttccac ccgtggtcac agcacctgcc gatgttacca tcactgcagg
    11701 tcagaactgc agggcatcct atactgtacc tcctgctacg attaccgaca attgcaacca
    11761 tcttggaaat gtatctgtga tccaccccaa tggagtaact aaccaaaacg gaggatttac
    11821 gattacaaat ctttcaatag gcacatacaa catcatttac tcggtcaatg acggctgtgg
    11881 acatattgtt cgggatacca caaaattgac cgttcaggac cttacagcac cggtagcaat
    11941 ctgcaaggag gctatcgtct ctctggacaa cacaggaaca ggaaggatgt ttgcctcttc
    12001 catcaacaat ggaagtcatg ataatggctg cggcccggtg actctcaagg tcaggaggat
    12061 gaatccggat tgcaatggtg cgcctgatca aactacatgg gcggattaca ttgactttta
    12121 ctgctgtgac ttaccgaaca atccgatcca ggtcattctt caggttacgg atgcaggtgg
    12181 cttgaccaat acttgtatga caacagtaac ggttcagaat aagaatacgc caatcattgc
    12241 ctatcccctt ccaaacatta ttgttccttg tggcacttca ttcgatgtca ataatctggc
    12301 actgactttt ggtgagtatg taaccaatcc ggctgatcgc caaaaaatca ttattgatgg
    12361 cgtagataaa ggctgggatg ggcttatcac gggcgtttgc acagcaacag taacagaaac
    12421 agcacctacc atcaatatcc cgcaatgtgg attcggaacc atcacccgac acttccttat
    12481 aacagacgga ggctcattcc aacaaacagt atcacaaacc attaccatca actatgacgg
    12541 tgtagaactt actacagctg atttcaatgc accattggac tatgttgtca caagtggcat
    12601 ttgcacacca cctgaccttg ccaatgcaga tctcggcagt ccgtatgtac ctaccttgaa
    12661 atcaccgttg aatggatgtc acaacataat gataaacaaa gaagacatcg tatttactaa
    12721 tgcttccaat gcatgtttca agatagtacg tacctggacc atcatcgact ggtgtcttgc
    12781 agcagccaaa ggtaatcaat atgctctgga tcatgctgtc aagtttacac aggccctggt
    12841 tgtaatgaat accaccgcgc ctacttttgt accacatgca gaccagattg ttgaaacaac
    12901 caactgtact gcaagcaatg ttacagtatc cacttcagcc accgatgact gtccgtcaaa
    12961 cttcctgacg tacagctggc aattggatct gaatttcaat aataatacca acacatggga
    13021 tcatcaggga acgggtagtt catttaccat caatatgcct attggcaagc accgagtgca
    13081 ttttgccgtt accgatgggt gtggaaatgt tgagcatcaa tattttaccg tagaggttaa
    13141 gaatgtcaaa caaccaacac cggtatatac caatttaacc gtagatttga tggcaaatct
    13201 tcaggtcacc ataccggcaa ggcttttcaa tcgtgcatca tttggcgcat gtcccaacag
    13261 ctatccgctg cgatttgctt acagttcgga tcctgctgac agcttacgga cctttgactg
    13321 ctcatttggt gtagacactg ctcatccggt caccatatat gtaatagatg ctaacggatt
    13381 gtttgatttt gctattacca atttaaccct caatgacaac attgttcagt gtccaaacac
    13441 catcactgtc atcagcggtc agatcgtaac tgaaggcaat gccggaattc cacaagtgga
    13501 agtaaatgca cattcctcca tgagcagatt gaccgatggg aatggtcaat tcagattcaa
    13561 caatgttcat ccgggagaaa catactatat cgagcctaag gatgataata atccgctgaa
    13621 tggtgtgacg acaggtgaca tcatcaagat ccagaatcat atccttggta agacatccct
    13681 gccaacgccc tataagatta tcgcagccga tgtcaatgag gacaaatcag tgtccatctc
    13741 cgatattgta atgataagaa agatgatcct tggaaaaatc gaccaattca gttccggaaa
    13801 aagctggaaa tttattggca aaaactacac attctctaat ccggctgcac cgctcaagga
    13861 aagttacccg caatccatac cggctgcaac aaacagttat ctggacggaa ttgacttcat
    13921 cggtgtcaag atgggtgatg tgaatggtga tttcacactc aactttgacg atccaaaggt
    13981 tgattcccgc tcatatgcta aattgtcaat tgaagatgta cgaatgaaag ccggcgaaat
    14041 tacaactgta catgttgtga atgccagcca gataaatgtg caaggtttac aggccaacct
    14101 ctcctttgca aagggagtaa aactggtagg cattgaaagt cagatgatgg aagccggtga
    14161 tgataatttc tatcaattca gtaatacatt gaaattgagc ctgacctcga aagacgtgct
    14221 caatgacaac ctgataataa ctttatacct ggttgcagac aatgatatgg atgtgtctga
    14281 tgctttccaa ctgaaccata ccggatttgc atccgaatta tacgacaacg aaggcaatgg
    14341 caatccaatt gaactgacat atacgaatag ccaatcgaat ctacaagtcg agcaaaatat
    14401 cccgaatcca ttcagcgacc agactcaaat ctcgtttacc ctgggcagcg ctgatcatgt
    14461 tactatgaag atttatgata ccaatggccg ggtgcttaag tatataaatg cattttacgc
    14521 caaaggcagg aatacgataa ccatcgataa tggatccata aatgcttccg gagtcatgtt
    14581 ctacgaaata acttccaatc gtaagacggt gaccaagaag atggtcaaat tgaaataata
    14641 gattagttta ggttttagaa cattttaatg ttcagagaag cccggtggtt gaaccgggct
    14701 tctttttttt caaaaaaatt ccttacattt tccaatcaac aagggaggcc gaatgcctat
    14761 cattaaggac aatattgtct ttaaggcttt gtaataacca atgtatcaca caacaggcat
    14821 catcagcaat aaatacgttt ttaccacttt agcttttttt tctacatttg caattcataa
    14881 atgcaagaaa tttggaaaaa caatacacca acaacatcat gatgatccgg cctgccaatt
    14941 ttatgtataa tgagcagact gctgtcacca atgcatttca gaccaatgat gaaggtgggc
    15001 gggcctccaa tgtcaacgta cttgccttac aggagtttga caattttgta ctgaagctta
    15061 aggaagccgg agtcaatgtt attgtaatgc acgactcttt gtcccctata aaaccggatg
    15121 caatttttcc caacaattgg gtcagtttcc atggcaatgg tacggtaatc acttacccta
    15181 tggcaactgt caacagaaga atagagaggc gggaagacat cattgatgcc cttggcctgg
    15241 tgttcaaggt caacagcagg tattcttttg aatcgtcaga ggaggataat cagtttcttg
    15301 aggggactgg cagtatgata ctggaccgtg agaacaaaat tgtatatgcc tgtttaagtc
    15361 cccgtactga tatatttcta cttgaccgct tttgcctgtt gatgggttac gggctagttt
    15421 cgttctattc cacggatcgt gaaggaaaag aaatatatca taccaatgtt atgatggcaa
    15481 ttgccgatca atacgcagtt gtttgcctcg atagcatcaa ggacgaggat aagagaatgg
    15541 aagtacggga tagtcttgag aagaccggga aggaagtcat agaacttact tttgaccagg
    15601 tgttacattt cgccggtaac atgttgcaag tggaaggtca tccgggaaaa agaatccttg
    15661 tcatgtcaca acaagcctac gatagtctga ccagagaaca aatagataaa ataaattcct
    15721 acaatccaat cctcgcagtc cccatcccaa cgattgaaaa atatggtgga ggtagcgtac
    15781 gatgcatgat ggctgaggta tttctaccca aaagataagt acttcccatc accccgaatg
    15841 aaaaaacgga tatactatga gtattagtgc aatgaaaaaa gcaacattat caggtgaatc
    15901 ttataagata atgatcgaag agaatgaaat accgactcat tggtacaata ttcttgccga
    15961 tatgaaggtt caaccgaatc cgacccttca accggaaaac agacagcctg taaccaggga
    16021 ttactacgct gatctgtatc cccagggctg tattgagcag gaattcagca cggaaaggtg
    16081 gattgaaatt cctgaagaag taagaaatat ttataaaatc tggcgaccca caccgcttgt
    16141 cagggctcac agactggaac aggcgttgca aacggactgt aggatatatt tcaagaatga
    16201 aggggtaagt ccggcaggca gtcacaagac caattcggcc attcctcaga tatattatgc
    16261 ggccagggat ggtgtgaaaa aagttgtaac tgagacaggc gccggacaat ggggaagtgc
    16321 agttgccttt gcagctcaaa tgtacaatct tgattgtgag gtgtttatgg taagagggcc
    16381 ttacaatcag aagccgagcc gccgtcaaat gatgcaactg tggggtgcac atgtaacagc
    16441 ctctcctagt gaacttactg cagtggggag gaatcaactt gcagaaaacc caaactcaaa
    16501 aggatctttg ggtactgcca tctccgaatc cattgaatat gcatcacagg ataaggatat
    16561 ccaatatgta ataggaagtg tgcagaattt cgttcttctg catcaaacag tcataggaca
    16621 ggaatgcctg aagcagtttg aaaaaatcga cgaatatccg gatgttgtag ttgctccatt
    16681 ggggggaggt gcaaattttg cgggaatttg ctttcccatc atgagagatt tgattcttgg
    16741 aaaaacaaaa acccgattcg tggcaagtga accattgact tgtccaaagc tctctgaagg
    16801 cgaattcagg tatgattatg gcgatgtcgg aaagaagaca cctctctttg ctatgtacac
    16861 tttgggacac gactttatgc ctgttccgaa tcaccttgtg ggcctaagat atcatggtgc
    16921 aggccccatt atatcacaat tggcaaagga aggacatatc gatgcgatgt cccacgacca
    16981 aaaggaatgt ctgcaagccg gcatattatt cgctaagacg gaaggcctga ttcctgcacc
    17041 ggaaagcact catgccattg cgtcagttat ccgggaagtc aacagagcta agcaggaagg
    17101 aaagagcaaa accattctgt tcaatctgtg tggtcatgga cttcttgacc tgtttgccta
    17161 tgatgactat cttaatggta aggtttcata gcattctaac tttaaaaact gaataggtcg
    17221 ggtatatttc cagcattcat aatttttagg gttaggccaa cgaagattca taacattcag
    17281 ataaaagatt gcccctaagg ggccatcgaa gaagttataa tcggcataaa accgggggac
    17341 agcgcatgaa tgccccattc aacttcgagc aaggtttaaa ttttgacaca gcctttattt
    17401 ttagcacctt caaatcaatc aacaataatt attaatgtat aatatgctta attactttta
    17461 tttataagca agttattctg tctacataca tttttcctgt tcagcttttc gtttggattg
    17521 aataaatgtg aaaattccgg aacacacgtt tattcctaat ttttttcaaa atgtatctca
    17581 acctattatc tttgaggtac aatcaaagat atgcgtattt atactaaaaa gggtgataaa
    17641 ggatccacat ccttgttcgg aggaaaaaga cttgccaagg atgcggtacg gatcgaagca
    17701 tatggcacag tggatgaatt gaacgctcag gtgggagttt tgcttgacaa cttggaaaat
    17761 gctgatattc aggatgattt acttcggatc caacactggt tattcgatta cggttccatg
    17821 ttggcaaccc caccaggagc caaactctat gtaaccccac ccggagaaaa ggcgctcaca
    17881 tttctggagg aaaagattga tgctatggag ctcgaattac caccattaaa gcatttcatc
    17941 ctaccgtccg ggcatacatc cacatcattt gcccatatgg ccagatgtgt gtgcaggagg
    18001 gctgaacggc ggattgtttc tctcagtcat gaagaggaaa ttggtgaaga gcctctggca
    18061 tttttcaaca ggttatccga ttatttcttc gtcctagcaa ggtatttaac tcatttggcc
    18121 ggtaaaaagg atgtagaatg gaatcccagg gattgaaata ggggcagaac aaaccattta
    18181 tcaataaaaa ctcaggctaa atcctttcta tggagcaaaa aataaatttc agtatattac
    18241 gaaacttaac tgcgtcatac aggatactca tcatcctgac ctctgctttg ataatcagcc
    18301 tcttgttacc ccgggtgaag actgttaact atgaatttga agagggaagt aaatggaaat
    18361 acagtgactt aagggcaccc tatgactttg cgatccgaaa gactgagaat gaaattgccg
    18421 ctgaaaagga agccatagtc aaaaacttta taccttatta taagaaaaat gaatccacaa
    18481 cggaaaacct gaatgataga atccttgaag aatacaatac ctatcaaagt catgccggcg
    18541 gggatacagc tctgtttctc agtaattaca aggatatcaa tatcaaacag atccgttctg
    18601 tttttcagaa aacttaccgg accggaatcc tggaagccaa tgatatttat aaggatcggg
    18661 aaaaggaatt tttgattcat attgtagagg gaaatatcat ttccctaaaa cccagggcag
    18721 ccattccaac agttgttcag gcaaagaatg agcttcatcg ggacatcagg caagccggtg
    18781 aacatggccc cctactctac cctgttatcg atgctctcat cgagcccaac atctttctgg
    18841 atcaaacctt gtccaatgaa tttctcaacc aggcattcaa gaacatagcc accaatgaag
    18901 gaattgttcg taaaggtgac atcataatca accgcaattc aaccatctca aaggatacct
    18961 atcagaagtt aatttctcta agagaattgg atgatgcagg taacacaggt aaattatcgt
    19021 atttcaagtc attccttgcg tattttgtct ttatttcagt tattctcatg ttctatgtgc
    19081 tttatgtacg gacctatgta cgcgaggttt ttcttatctt caagaagatg ctttttatgc
    19141 atctttggct cattctatat gtctttatga cctatttcgt catcaagtca tccagtcttg
    19201 acctactgat gattcctttc tgcatagcac ccatagtcat taagaatttt tataatcagc
    19261 gacttgcatt ttttacattg gtagccatcc tgaccatcgt tgccttgttc ggtccggtaa
    19321 caaaggattt catcatcttg cagataacag ccggaataat ggttgtgatt gtaacaaaag
    19381 aaaccagata ttggtccaaa tttttccttt tgctgctatt tcttttaggt acgtatatct
    19441 ttacttatct ctttcttacc tatatcaaaa acaacaacct cgcagacttt aatttgaaca
    19501 gtatttggtg gatctgcctg aacgttttcc tcacgttgct tgcctatccg ctgatcccgt
    19561 tactcgagcg attattcgga ttcacatccg gaattacgct tgcagagcta tccgacctga
    19621 acaacaattt gctgaaagag ctttccataa aagcaccggg gactttccag catgtattgc
    19681 atgtagctac acttgcagag gcagcagcag aaaagatcgg agccaatagt tctatggcac
    19741 gggtgggagc attgtatcat gacattggca aaatgaaagc accctataat tttaccgaaa
    19801 atcaaaccaa catcaatcct cacgactcca tagatcatgt caccagtgcc aagcttatta
    19861 tcgaacatgt tccggaaggg gttaaaatcg caaaaaagaa tggtctgccc aacgtaatca
    19921 ttgatttcat caagacccat catggtacga caaaggtgga gtatttctac aggaaatttg
    19981 tcaaggaaca tgccgatcat gctgttgacg agaaggtatt tacctatccc ggacccagac
    20041 cacgtacgaa agaacaggta gttgtcatga tggccgatac gctggaagcc gccgtcaaga
    20101 gtctgatcaa ccctacagaa gaagaaatca atgatatggt ggacaaatta attcgtgcaa
    20161 agattgaaga tgggcaattc gacaggtctg atataacctt cgaagatgta cagacctgta
    20221 tccgcatttt taaaaacatg ctggtcaata tgagtcatat cagaatcgct tatcccgatc
    20281 cacgacctga aaccaattga agtcgcgact tttgctcacc ctgataagat tgtaacaata
    20341 tcatgattaa tataaataca aatgaaagta ctccaataga agtttcaaag gttggttcaa
    20401 agaaaaaagt gactaaataa agaatcacta agagatgaaa taggaataac tttcggtgta
    20461 ttggattcct ccatagttgg taccaaaaca acaaggtgaa agcaagggag agcaatcccc
    20521 cgcacactaa ggtcatcagc cattgattat ggggaatctg acttcccgga taatgattct
    20581 tgatggcatt ctgctccaat tgtcgatgca aatcacccga gcccactccc agccatttat
    20641 tttccttcca cagcgaccac ccgttttgca aagaatatag ccgctcacta tcagaatact
    20701 cacttccctt atcttccctg aacttccgcc aatcatacag ggtataacct gcttttatct
    20761 ttaccgaagg gataaataaa tatgcaagaa tacttcctcc gattgctgca atgatgacaa
    20821 tactgattag cgttattctt ctcttctgca tgataaaata agccaaaatg atcagcaagc
    20881 ctaaaaaact cacaagccaa gcagtacgga ccgaaagaaa cagaatggaa aagcccagat
    20941 acaaggtagt caataaaaca aatacctgcc atcttacagg ataagtttta ctcagaaaag
    21001 caaagaggcc tcccaaaaaa gtaaaactca gaaacatacc atatcggatg tgcggaatag
    21061 gtgtcggtat tggttt
//
LOCUS       DAOQQN010000007        20614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_775_length_20614_cov_5.586604, whole genome shotgun sequence.
ACCESSION   DAOQQN010000007 DAOQQN010000000
VERSION     DAOQQN010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 20614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20614
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_775_length_20614_cov_5.586604"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /gene="gcvT"
                     /locus_tag="PLG24_00580"
     CDS             complement(<1..1067)
                     /gene="gcvT"
                     /locus_tag="PLG24_00580"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HMZ23403.1"
                     /translation="MKETPLTSKHISLGAKMAEFAGYNMPISYKGIIDEHKAVRNNVG
                     VFDVSHMGEFTVKGKDAEAFLQNLTSNDVSALRPGAIQYSCLPNKTGGIVDDLLVYRM
                     DDEADGSNSYLLVVNAGNMSKDWEWINAHKSGDVRLDDISEQTALLAIQGPKATAVLQ
                     KLTSINLSEIPYYHFRIGTVAGVENVIVSATGYTGSGGFEVYMPNEHIEKLWDQLFDI
                     GDEYGIMPAGLGARDTLRLEMGFCLYGNDIDETTSPMEAGLGWITKLKKGVFNSSEIF
                     KDQKEVGVSKKLIGFKLKERRVPRHDYRILNEEGGLIGRVTSGTMSPSLEYPIGMGYV
                     ETKYAMPGTVIQIEINNKLFAA"
     gene            complement(1074..3077)
                     /locus_tag="PLG24_00585"
     CDS             complement(1074..3077)
                     /locus_tag="PLG24_00585"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMZ23404.1"
                     /translation="MKAKFLFWIIILIVGITSCIPNKPNNNLNFSLDLKEKDIQLAYE
                     AGYNHSRDSLLRYFHHKNPTVRYIAVNAFASWKDTTVIDSIAPLLKDEDENVRCGAAY
                     SLGQLRSLKAEPYLISAFVTFDSLNKYQLFNATILEALGKCGSQNTLKQIASVKSYLT
                     TDSLLLIGQSKSIYRFALRDIILPQGTQVCINMVCNPSYPTESRLYAANYLSRAKGID
                     MKTNLDSIIYAYHQSTNRDFKLALATAITKTKNEKSFSFMKSEMQTAQDQGIRFNLLS
                     NLKTFSYEEADTVAFEYLKDKNFRIARLAAKYLIDHGKETDAKKYLEYYFNPETNSKA
                     HIEILGAANKHLPFTMTMSRTTVNDILKDSLLKVSDVYRKADIINALAYDVTNFLFIR
                     NLTVKAPDKILQIVGLECFGSILSNPNFAKTYRSNYVPYKKQIFSYLVDGILSKDVGL
                     VSTAAVIMRDPVLNLRNFLPSDSLFRIALNKQKLPENVEAYNELAKTISYWSGQPYKP
                     APDKGFRMLDWAVLNNISDSTLCEIITTKGTIKMLLQPSLAPMTTANWIELVKRNYFN
                     GKNFHRVVPNFVIQSGCNRGDGFGAMNYIIRSEYSRAFYNRAGMVGMASAGPDTESSQ
                     FFITCAATPHLDGRYTIFAYVKEGMNIVETIQRGDKILKVNIK"
     gene            complement(3200..5263)
                     /gene="feoB"
                     /locus_tag="PLG24_00590"
     CDS             complement(3200..5263)
                     /gene="feoB"
                     /locus_tag="PLG24_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HMZ23405.1"
                     /translation="MGNNINICLAGNPNSGKSSLFNALTGLNQKTANFPGVTVDKKAG
                     KLFLPDGKPANLIDFPGAYSLHAITMDEQIFTKIMLNPESPNYPDIICFVVNSELVDR
                     QLLLGSQIRDLGIPMIVVLTMSDLVQLDEKKIHKVSSFFGCPVVQCSSRTGEGLDTLK
                     QAILDQRNKSTAGKTINSNAEKYVNGLLSSGLSVDKNSITDYQSFLKVLESNKSENGK
                     NVLKAEVWDTLGRYDELSDLISILKPAVTKIPPVQNKIDKILIHPLWGMLNFAAIMFL
                     VFQSIFSWAEKPIEWLEYGFALSAELIETHLPQNWFSELLSNGIIPGLSGIIVFVPQI
                     FFLFLFISIMEESGYMSRVVYLFDYWMRKIGLNGRSTIALISGGACAIPAIMSTRTIS
                     DWKERLITILITPMISCSARLPVYSLLIVFIIPANLQYFGFINARGVAFLGIYIFSTL
                     AALFAAFVLNKLIRNKGKSQLMLELPNYRMPIAKNVAFNVWEKVKSFIKEAGKVIFII
                     SIVLWFMTSHGPSERRENALEAYRKEMKSNPPTEQSHVNESSILLENSYAGILGQYLE
                     PVIKPLGFDWKIGIALITSFAAREVFVGTLATIYSAQDDGDNPEKLSQMLNSQINPNT
                     GEKVFNVATSASLIMFYMFALQCLSTVAVVKKETNGWKWPTIQLISYTILAYLISLLT
                     YQLLA"
     gene            complement(5253..5492)
                     /locus_tag="PLG24_00595"
     CDS             complement(5253..5492)
                     /locus_tag="PLG24_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HMZ23406.1"
                     /translation="MTTLDKLNAGDYCRIISYSDDSIASKLISMGVFPNAEGFLLDTN
                     HKKHTFFLIVGSLKLGLLYNEAASIIVHKIENNGE"
     gene            complement(5657..8092)
                     /locus_tag="PLG24_00600"
     CDS             complement(5657..8092)
                     /locus_tag="PLG24_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HMZ23407.1"
                     /translation="MPKTLQYLYAVLLLIAVANPAQSQSNEYVEHFNIFASDSVIVSI
                     PRYDNSFFYTKIAKYGSTDQVLSTGEEFVIRYKPYAGHFGLDTFQVVYFYPNPSNGEL
                     AKKSKKVIIKTSQFILNNDQFTVYTNDLNFELDVLANDLIPGGGAKITYLPLNSQSQL
                     SISNDSSKIIYNGCNGAVSENLSYQVTKNGYKELGRFKLNVLDTTINILPQEFKKFLV
                     KNSSINFTHPYAVANISKSVSHGTLAQNGLSWTFSPTENYIGLDTLEFEVTNGISTAL
                     HRFIFKVFDAATPNILVKDDYFYSLKGQYVVCDLLKNDFDMVLNVSLSVQPQNGSLVK
                     MANGVYKYTPNIGFEGVDVFVYNACDLASNVCEYGTVTVTVSNFTPEPVYNLSTTKGS
                     PIKIFYPFPTSGYKVSVIPSAYPRNGIVYVVDNQKNLDYSPAVNYVGLDSMKIKYTLL
                     SNSSIFHIVKIYIQIFDLPPSCYANCVWPGDHNNDGRVDMKDLTFIAPFIGESGAPRN
                     NNTQNEYWVGQDAADWDISSNNTNMKYGDGDGDGLILEQDTAMMNKYYRNLHGLFVNK
                     GVNSPFLPFNLRSDKDFYEPGDSITMTFSIGSENTPVLNLSAFTVSLNFSEAFNDQSL
                     TTDFKPNHWLSDGDKMLQMAKKPIVRNIDLGAGRVSGTGTAGYGDIAVIKGIVDDELD
                     GFKSDEGIFYARIQLNDATLVIDGCDELRTAQQIFEFPIKIQKSTSNSTRKLINLYPN
                     PTNSHLTLKAYNKDNIINSYHVYTITGQSIMSKGNVNQNQVDINLEGFHPGIYIVKTF
                     TSVGVEINKIEKL"
     gene            8401..9660
                     /locus_tag="PLG24_00605"
     CDS             8401..9660
                     /locus_tag="PLG24_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23408.1"
                     /translation="MDVWNDIKPNEEYNDVKFRKYLSDLLKLLLEFFAVEELRGRSIL
                     SSQLLLEYANKKKIPKLINTSVQVSQHTSNKFYNRSSDYYLYHFQIEKNLFYLQEGNI
                     ELYAKKNVENIAENLDKFYIAEKLKYYCEILGREALSNIQYNFNFISELLSFVENNDF
                     DNDLIIRIYYLIYKTLSTPDESEYYYQLKAILFENYRLFDGFELQELFGSILNYCIAQ
                     INIGKQDFQDEMISIYDFIFEKNILLNENYLTPAKFKNVVTTALRVGNYDFAESFIKE
                     YDNLLPVTQKESIVTFSLAQIYFYKKEFGQVIKQLQKVEYPDLITNLRAKTIYLATLL
                     ELKEYDAISMWSESFKAFLNRKKKEIPAARIKSYQNFITIVRLIAGAEFGDKKFYHRV
                     IKELDKFSGNIVNEQWLREKLSERFRM"
     gene            9672..10661
                     /locus_tag="PLG24_00610"
     CDS             9672..10661
                     /locus_tag="PLG24_00610"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HMZ23409.1"
                     /translation="MTKLAQRIDWWKVLKWLVLPVFIAVLYYQLTSLQQKPDLLNRLR
                     VFLAEENIIIIVFVLPLLTFFNYALEAAKWQVLVKPAQRRLYSEALNDVLIGILFSVL
                     TPARLGELAGRVVNLRMGNKLVGAASSLAGSIGQNIVIFFLGSVGFVFFALIIGHLGT
                     WPAVGSLFLVFCLTGLFLFLFFNLYLLIPIVNRVSLPKRWKQLTEKLYALKDYSNTDL
                     LKVLLFSLLRVAIWSCQYLIILWMLIPDIDWQVALLVSWIVFLIQTGIPLPPISSLVA
                     RSSIAIYLWSYIGISEWNALTASFVLYFNNLIIPSVVGLIVIYLNKKSFNANI"
     gene            10648..11487
                     /locus_tag="PLG24_00615"
     CDS             10648..11487
                     /locus_tag="PLG24_00615"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HMZ23410.1"
                     /translation="MRIFSFFATALLFILPLKSGSNQVDGYSNIAISTCSGDNVTFRD
                     SEELTYKIYYNWGLVWLSAGEATFRVQEQKNGYLISAVGKTYPAYEWLYSVNDRYEVF
                     VDKSTLLPIWSVRDIRENNYTQYERMDFNQSTGKVTSRRGKNKSETTTAVISVNDCTH
                     DILSILYFVRNYPFNTFRNGQVFPVNVFLDRENNNLTAKYKGIVKKHNVRGLGNFNSF
                     VITPATIEGKVFEKGNTMKMYISEDENQIPLMVETSLKVGNVKVILKSYKNLRYSLKS
                     KVN"
     gene            11575..12168
                     /locus_tag="PLG24_00620"
     CDS             11575..12168
                     /locus_tag="PLG24_00620"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HMZ23411.1"
                     /translation="MVKYLLGILLILFLFFGGYFFSEFLQNKREEKTKVSNEVILNQI
                     KEVAKLVSVEGYFTEIYKYKNYYNLDWSIFTKRALVRVKAKVSMGIDLNKMKFDFDET
                     SKTLTIGPMPQPELFSIDHDIDYYDISEGTFNSFSSDDFNKINNDVKQYIRNVALKSE
                     LPQRAKLQSQKTLETIRILAETAGWKVIEEGIAPEKG"
     gene            complement(12174..14561)
                     /locus_tag="PLG24_00625"
     CDS             complement(12174..14561)
                     /locus_tag="PLG24_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HMZ23412.1"
                     /translation="MFYPNDIFQVLEFDRLLILAESYCKGEAGKRLLKNVRFHTNPDE
                     IERLLSETMEFKLSLDAGDSFPTGNYTDIDTFLKYLKITDYFLEVEHINELRSVLEIM
                     KNLDRFASSSGNTKNYPVLTQKILDQGYIPTYLQQINQILDDQGKVKNNASEDLVKIR
                     RAISAKKSELERAFDQIAAQYKSKGLLSESSESYRNGRRVLSVPAEHKRKIPGIIHDE
                     SETGKTVYIEPEPVILLNNDLFELQHEELREIVRIIKKLCNNLATGYDDFVRYLDCVA
                     YLDFVGAKAQLAKLYGGDKPQIVSHPNLNFIKAFHPLLYIKNHKTGKKTIPFDLNMSD
                     NNRILLISGPNAGGKSILMKAVGLIQVMAQCGFLLPVYKESKLGIFKNIFADIGDKQS
                     LEEDLSTYSSHLKSMKFFTDQTNSETLILIDEFGTGTDPDVGGAIAESVLAFLNRKKC
                     MGVITTHYSTLKLFAHNNSGVTNGSMLFDSNGLKPTYVFKMGSPGSSFAFELATSNGL
                     PAVVLHHAEKKLGSKKYKVDRLLNDLQSDKNKLNKQITDLEKKQQQLDKLIANYERQL
                     AEYEFKRKKFRQEVKEFQLKSINAQNEFIEDQIRELSKEKDLDQIRELALKKRAERER
                     LVAEVNSLHNETLRISKTAMHNKPIETGDFVRLKSGDTTGTVEEILKDKAKVSVGGLT
                     VLIPISQLIAERKPQDLRRVKGVFTDILSDVSKVKDKIDIRGLRVDEAMQQLQEFFDQ
                     ALIAGKINLEILHGKGDGTLKRIVKQKLKEYNLPMQISHPAPDAGGDGITLVHIH"
     gene            complement(14633..16957)
                     /locus_tag="PLG24_00630"
     CDS             complement(14633..16957)
                     /locus_tag="PLG24_00630"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HMZ23413.1"
                     /translation="MRHKLANQRVNTVEENSLWAASVERSDTFDILDYDLMIDLRQMG
                     QNILTGKAIVTFTPKMVGLDYIPLDLLKLDVDSVFFEGVPATYDYNRILLNIYAGYQL
                     EAGKSYNVTVHYHGTPKTSTSGFGGFYFEDGIAYNLGIGITDDPHNFGRSWFPCFDNF
                     VERSTYTYRIITNNNITSYSVGRLIDEQPFNDNSKIRVFRMDQQIPTYLSAIAASTYV
                     SYEYNHDGIYGEVPVQLVSKPSDQARVINAFSTLGTAIDALESWYGPYPFERVGYVMT
                     TRGAMEHPNCIAYPVSSTSGNAADRRLFSHELCHLWWGDLLTLRTQADMWIKEGNAEY
                     GAHLFDEYLNGRQSFERLVSENKYDVITTAHISDGRYLPLSPMPKDITYGATTYKKGA
                     MVMHSLRGYLGDDLFRTAQQKVLKDYAYSNLTAYSYRDALSAASGVDLTDFFNRWVFD
                     IGFYDFVISDFQEIDFSSYTHKIKIKQKLLKSNGFCKDAPVVLTFVDQDNNLVNQRVT
                     TSGEESEILLNLAFQPKAVFLNYPMALNLAQFSSLQNVKKTGSVTFTYSNLSISAAEV
                     KDSTYAFLENHYTAPDAGTNPAIRVNNKHFWKFTVADPDHVSFTGKIEYNLNWDSDLL
                     QNGEDSIQLFYRPSGVEEWQIYPYYSKKGGSQSDKKGSFLISNFLAGEYTTGNIDPSL
                     LSKNEIPVSSGRIYPNPARDEVSFSFIGINPSTNNEYHFSLTDMLGMTYYDRQVSGNE
                     NTIPLSAGLTPGLYIATVRDKYNRILISDKLFIQ"
     gene            complement(17056..18450)
                     /locus_tag="PLG24_00635"
     CDS             complement(17056..18450)
                     /locus_tag="PLG24_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HMZ23414.1"
                     /translation="MIKIKFGTDGWRAIIAREYTVDNVQRVAAGTAAWMKQSGMKTVV
                     VGHDCRFGGEMFLKAITEVFCFHGIKVLYGKGFVSTPMVSLGVVKLKADLGIVITASH
                     NPPSYNGYKLKSHFGGPSSPADITSVENLIPDEWNTTTATFEDYVQQNMAQEVPLEDI
                     YVNHAESCFDLDLIRNSGLKIAYDAMYGAGQRAMLRLLPDVMALHCDDNPSFRGQAPE
                     PIAKNLKELSELISSKDDISIGLANDGDADRIGMFDSKGKFVDAHHILLLLLLYQYKY
                     KKLTGEVVITFSVTDKMRELANKYGLPCEITKIGFKYIAEIMTTRDVLVGGEESGGLA
                     VKGHIPERDGIWIGLMILEFMASTGKSLEALIQELYEEVGAFWFDRDDLHISEQQKQN
                     VIKACKEGQYTSFGPNKVISTEDLDGFKYIFGDERWVMIRPSGTEPVLRVYAQGKDVA
                     DTRNMLDAVKTTIL"
     gene            complement(18707..19237)
                     /locus_tag="PLG24_00640"
     CDS             complement(18707..19237)
                     /locus_tag="PLG24_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HMZ23415.1"
                     /translation="MKSILLKSIVALFLLATIPSVSNARNVTNEASIPPFLLNTLNMM
                     FNVSAKVKIRANVVDFASKFLGTPYRSGGTSPKGFDCSGFVGYVFNEYGYDLAHSSRT
                     LSTIGVSVKKEEARPGDLVFFAAGGRVHHVAIVYSNYNNDLKIIHSSNSKGVTIDAIN
                     SSAYWSSRFYSIKRII"
     gene            complement(19748..>20614)
                     /gene="gldN"
                     /locus_tag="PLG24_00645"
     CDS             complement(19748..>20614)
                     /gene="gldN"
                     /locus_tag="PLG24_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HMZ23416.1"
                     /translation="SATNSSTKSTTPKTTGPKTSSPVIVAAQDTVRKPDPDAPMDDII
                     KRDMIKQKLVLAYEPLNERDIFWERRIWRVIDCREKMNLPFKYPDHEFYTILKKGIEN
                     GDIRCYTDESFQFRASTEDINKTVSGIDTSEVYNPETGQYESVITKNDFDPQLINQFR
                     VKEVWYFDSESSTMKVRILGIAPLYVKIDQTTGVALPAMPLFWIYYPDCRKYLSQHQV
                     FNEANIASPVSWDDMFENRHFSSFITKKSNVNDFRLVDYDNLRENGVDMLLESNKIKE
                     EIFNFEHDLWSY"
BASE COUNT         6093 a         3897 c         3888 g         6736 t
ORIGIN      
        1 gctgcaaaaa gtttattgtt gatttcaatt tgaatgactg ttcccggcat agcatatttt
       61 gtctcaacat aacccattcc tatcggatat tccaatgaag gagacatcgt tcctgaggta
      121 acccttccta ttagccctcc ttcttcattc aggattctgt aatcatgtcg ggggactctt
      181 cgctccttta atttaaagcc tatcaatttt ttagatacgc ctacctcttt ttgatcctta
      241 aaaatttccg atgagttgaa aactcctttt ttcaattttg tgatccatcc aagtcctgcc
      301 tccatcggag atgttgtttc atcgatgtcg ttgccataca ggcaaaagcc catttctaat
      361 cgcaaagtat cccttgctcc aagccccgca ggcataatac cgtattcatc acctatgtca
      421 aacaactgat cccatagttt ttcaatatgt tcattgggca tatatacttc aaaacctcct
      481 gaaccggtat agcccgtcgc tgaaacaatg acattttcaa cacctgcaac cgttccaatt
      541 ctaaaatggt aatacggtat ttcggataga ttgattgatg tcagtttttg taatacggct
      601 gtggcctttg ggccctgaat ggccaaaagt gcagtctgct cagatatatc atccaatctt
      661 acatcacctg atttatgcgc attgatccac tcccagtctt tggacatatt tccggcattc
      721 actaccaata aataggaatt tgaaccatcg gcttcatcgt ccattcgata caccaggaga
      781 tcatcgacta ttccacctgt cttgttggga aggcaggaat attgaatcgc tcccggcctt
      841 agggcactta catcatttga tgtcaaattt tgaaggaacg cctctgcatc cttccccttg
      901 acggtaaatt ctcccatatg gcttacatca aataccccaa cattattcct tacagctttg
      961 tgctcgtcaa tgataccctt gtaagatata ggcatattat aacctgcaaa ttcagccatt
     1021 ttggctccta gtgaaatgtg tttcgatgtc aatggcgttt ccttcatgat catttatttt
     1081 atattaactt ttaatatctt atctcctctt tgtatggttt cgacaatatt cattccctct
     1141 ttaacatagg caaagatagt atatctccca tcaagatggg gtgttgccgc acaagtaata
     1201 aaaaattggg aagattcggt atcaggtcct gcagaagcca ttcccaccat tcctgctctg
     1261 ttataaaacg cccgactgta ttccgaacgg ataatgtaat tcatggcacc gaatccatct
     1321 cctctgttac atccactttg aatgacaaag ttgggtacga ctctgtggaa attctttcca
     1381 ttgaaatagt ttcttttgac taattctatc cagtttgcgg tagtcatagg ggccagtgat
     1441 ggctgaagca acatctttat tgttcctttg gtagttatga tttcacataa agtactgtct
     1501 gagatattgt tcaatacagc ccagtcaagc attctgaatc ccttgtcagg tgccggctta
     1561 taaggctgac cactccagta tgaaatggtt tttgccagtt cattgtatgc ttcgacattt
     1621 tcgggaagtt tctgtttgtt caatgcgatg cggaatagag aatcacttgg cagaaaattt
     1681 cttagattta atacaggatc cctcattata acagctgccg ttgataccaa accgacatct
     1741 ttggagagta taccatcaac caaatatgaa aaaatctgct ttttataggg tacatagtta
     1801 gatcgatatg tcttggcaaa gttgggattg cttaaaatac tgccaaagca ctccaggcct
     1861 acaatctgca ggattttgtc aggggctttg accgttaaat ttctgataaa taaaaagttg
     1921 gttacatcat atgcaagagc attgatgata tctgctttcc tatagacatc ggatactttc
     1981 agcaagctat ctttcaggat gtcattgacg gtcgtccttg acattgtcat ggtgaaggga
     2041 aggtgtttgt ttgctgctcc taaaatttct atatgggcct tagaatttgt ttctggatta
     2101 aaataatact ccaaatattt tttcgcatct gtttcttttc catggtcaat taaatatttt
     2161 gctgctagcc ttgctatacg aaaattcttg tcctttaaat attcaaaagc aactgtgtca
     2221 gcttcttcat aagaaaaagt ctttaaattg ctcaataggt taaatcttat accttgatct
     2281 tgagcagtct gcatttcaga tttcatgaaa gagaatgatt tttcattctt tgtcttggta
     2341 atcgctgtag ctaaggctaa tttgaaatca cggttggtgc tttggtgata agcgtatatg
     2401 atagaatcca gatttgtctt catatctatg cctttagccc tggaaagata gttggcggca
     2461 taaagtctgg attctgtggg atatgaagga ttgcatacca tattaataca gacttgggtt
     2521 ccttgtggta aaatgatgtc tctcaaagca aatcgatata ttgattttga ctgtccaatc
     2581 aataaaagac tatctgttgt aagataggac ttgacagacg cgatttgctt taaagtgttt
     2641 tggctgccgc acttacccaa tgcttccaga attgtggcat tgaacaactg atacttattg
     2701 agactatcaa atgtaacaaa tgcagatata aggtatggtt cagccttcag gcttcttaat
     2761 tgtccaaggc tataggctgc gccgcaccgt acattctcat cttcatcttt caatagaggg
     2821 gctattgaat cgatgaccgt cgtatccttc caggatgcaa aggcattgac cgcaatgtat
     2881 cgtacagttg ggtttttgtg atgaaaatat cggaggaggc tatctctgga atgattgtat
     2941 ccggcctcat atgccagttg tatatctttc tccttgaggt caagagaaaa attgaggtta
     3001 ttgttgggtt tgtttggaat acatgatgtt atacccacta tcaggataat gatccaaaaa
     3061 agaaattttg ctttcatcaa tttaatttgt accaggaatt aggcaattga gccttgttag
     3121 atatcaacac ttaccttaaa gtgttgctta cctgatgcaa atataatact atgaattgat
     3181 tagcacataa attgagatat cacgcaagca actgataggt cagtaaactg atgagatacg
     3241 ccaaaatagt atatgatatt aattgtatgg taggccattt ccaaccattt gtttcttttt
     3301 ttaccactgc tacggtactc aagcattgca atgcaaacat ataaaacatg atcagtgaag
     3361 cagaagtggc cacattaaag actttttcac ctgtgtttgg atttatttga ctgttgagca
     3421 tctgagatag tttttccggg ttgtcaccat catcttgtgc actataaata gttgccaatg
     3481 tacctacgaa tacttctctt gctgcaaagg aagtaatcag cgctattccg attttccagt
     3541 caaagcctaa tggcttgata acaggttcca ggtattgacc caggattccg gcataggagt
     3601 tctccagtaa tatggaagat tcgttgacat ggctttgttc agttggagga tttgacttca
     3661 tttccttacg ataggcttcc agcgcgttct ctcgtctctc cgatggtcca tgactagtca
     3721 taaaccatag gactatactg atgatgaaga tgactttacc ggcttctttg ataaaggatt
     3781 ttactttttc ccagacattg aatgcaacat tcttggctat cggcattcta taattgggta
     3841 attcaagcat caactggctt ttacccttgt tgcggataag tttattcagc acgaatgccg
     3901 caaataatgc cgctaacgtg ctgaagatgt aaatccccag aaatgcaacc ccacgtgcat
     3961 tgatgaatcc aaagtattgc agattggcgg gtatgataaa tacaatcaga agtgagtaga
     4021 ccggcaatct ggcagaacaa cttatcattg gtgtgatgag tattgtgatc agtctttcct
     4081 tccagtcact tatggttctg gtactcatga tggctgggat ggcgcacgcc ccaccggaaa
     4141 tgagggcaat ggtactcctg ccattcagtc ctatttttcg catccaataa tcgaatagat
     4201 acacaactcg gctcatatac ccagactctt ccatgataga aataaataaa aacaggaaga
     4261 atatctgagg cacgaataca ataataccac ttaggccggg gatgatccca ttagacaaaa
     4321 gttcactgaa ccaattttgg ggcaaatgag tttcaattaa ttcagcagaa agagcaaatc
     4381 catactccaa ccattctatc ggcttttctg cccatgaaaa tattgattga aaaacaagaa
     4441 acatgatggc ggcaaaattc agcatccccc ataaaggatg tatcaatatc ttatctatct
     4501 tattctgaac aggtggaatt ttggtaactg caggctttaa aattgaaatt agatcactta
     4561 actcatcata acgacccaat gtatcccaaa cttcagcctt tagaacattc tttccgtttt
     4621 ctgatttgtt tgattcaaga accttgagaa aggattggta atctgtaata gaattcttgt
     4681 caacacttaa acctgatgac aataatccat ttacatattt ctctgcgttg gaattgattg
     4741 tctttcctgc ggttgacttg tttctttggt caagaatagc ttgtttgagt gtgtctaatc
     4801 cttcaccggt cctgctactg cattgaacaa ccggacatcc gaagaatgaa gagaccttgt
     4861 gtattttttt ttcatccagt tggacaaggt cactcatggt gagcacaact atcatcggta
     4921 tgcctaagtc ccgaatctga cttcccagta ataattgacg atcaacaagc tctgaattga
     4981 ctacgaagca aatgatatcc ggataatttg ggctttccgg attaagcatg atttttgtaa
     5041 atatctgctc gtccattgta atggcatgaa gactatatgc ccctggaaag tcgattaaat
     5101 tggccggttt gccgtcaggt agaaataatt ttccggcttt tttgtcgaca gttacgcccg
     5161 gaaaattagc agttttttgg tttaatcctg tcaatgcatt gaaaaggctt gattttccac
     5221 tattgggatt tcctgccaga caaatattta tattattccc cattattttc tattttatgg
     5281 acgatgattg aagctgcctc attatacaaa agaccaagtt ttaatgagcc gactatcaga
     5341 aaaaaagtgt gtttcttatg attggtatcc aacagaaatc cttctgcatt tggaaataca
     5401 cccatactga tcaatttact ggctattgag tcatcagaat aggaaatgat tctacaatag
     5461 tcccctgcat ttaatttatc taaagtcgtc atgtaatctc ctccctttca tttatttaga
     5521 ttaattctaa gttgtttcga agcgccaaaa aaaagtgagt cgccgcaaca actcactacg
     5581 aataatgaac aaatccaaaa ttactcttaa aatggaagtt ccaaattaat tgtttttttt
     5641 attatataat aattatttat aacttttcta tcttatttat ttccacgcct acagaagtaa
     5701 atgtcttcac aatataaatt ccgggatgga atccttccaa attaatatca acctggttct
     5761 gattaacatt acccttagac attatactct gaccggtaat agtatataca tgataactat
     5821 ttattatgtt atctttgtta taggctttta atgtcaaatg actattggtt ggattcgggt
     5881 agagattaat cagttttctt gttgaattag atgttgattt ctgaattttt atagggaatt
     5941 caaaaatctg ttgtgcggtt ctcaactcat cacaaccatc aatgaccaat gttgcatcat
     6001 tcagttggat gcgtgcatag aagattcctt catcgctttt aaatccatcc agttcatcat
     6061 cgacgatgcc tttaattaca gctatatctc catagcccgc cgtgccggta ccgcttactc
     6121 ttcctgctcc caggtcaata tttcttacga ttggtttttt ggccatttga agcattttgt
     6181 caccatcact caaccaatga tttggcttaa agtctgttgt aaggctttga tcattgaagg
     6241 cttcactaaa attgaggctt acagtaaagg ctgaaagatt taacaccggc gtgttttctg
     6301 accctattga aaaagtcatt gttatcgaat cacccggttc gtaaaaatcc ttatcgcttc
     6361 tcaggttaaa tggaagaaaa ggtgaattaa cccctttatt gacaaatagt ccatgcaggt
     6421 tccggtaata tttattcatc atggccgtat cttgttccag gattaggccg tcaccatcac
     6481 catctccata cttcatgttg gtattattac tggaaatatc ccagtcagca gcatcttgcc
     6541 ccacccaata ttcattctga gtattgttgt tgcgtggtgc accactctca ccgataaaag
     6601 gtgcgatgaa ggttaaatcc ttcatatcca cccgaccgtc attattgtga tcgcccggcc
     6661 atacacaatt ggcataacag cttggtggta agtcaaatat ttgaatataa atttttacta
     6721 tatggaagat ggaagaattg gataataagg tatattttat tttcatactg tccaggccga
     6781 cataattgac tgctggacta taatcaagat ttttctggtt atcgactaca tacactattc
     6841 catttctcgg gtaggcggaa ggtatcacag atactttata accagaagtg gggaaagggt
     6901 agaatatctt aattggagat cctttggtcg tagaaagatt atagaccggt tcaggtgtaa
     6961 agttgctgac tgtaacggtc acagtaccat attcacaaac attagacgca agatcacatg
     7021 cattatatac aaagacatca accccttcga aaccaatatt tggagtatat ttatatacac
     7081 cattggccat ttttaccaga ctaccgtttt gtggttgaac gcttaaactg acattcaata
     7141 ccatatcgaa gtcatttttc aataggtcgc atactacata ttgacctttg agtgagtaga
     7201 aataatcatc tttgaccagt atattcggtg tagctgcatc aaagaccttg aatataaatc
     7261 gatgcaatgc agtacttatt ccgttggtaa cttcaaattc cagtgtgtct aaacctatat
     7321 agttttcagt aggtgaaaaa gtccagctta atccattttg tgccaatgtt ccgtgagaaa
     7381 ctgatttact gatattcgcc actgcatatg gatgagtgaa attgatactt gaatttttaa
     7441 caagaaactt tttgaattcc tgaggaagga tattaatagt tgtatccaga acatttagct
     7501 tgaaccggcc caattcttta taaccatttt tggtaacctg ataacttaga ttttcagaca
     7561 cagcgccgtt gcaaccgttg taaattatct ttgaactatc attgctaatg ctgagttggg
     7621 attggctatt cagaggtaaa taggtgatct ttgcacctcc tccggggatg aggtcattgg
     7681 ccaatacgtc taattcaaag ttcaggtcat tggtatagac tgtaaattgg tcattgttaa
     7741 ggataaattg agaagtcttt attataactt ttttggattt cttagctaat tccccgttgc
     7801 ttggattggg ataaaaatag actacctgaa atgtatccaa tccgaaatga cctgcatagg
     7861 gtttatatct gatgacaaat tcctctccgg tggacaaaac ctgatccgtt gagccatatt
     7921 tggctatttt agtataaaaa aaggaattat catatcgtgg aatggaaact atgaccgagt
     7981 ctgatgcgaa tatgttgaaa tgctcaacgt attcattcga ttgcgattgt gcaggattgg
     8041 caactgcaat cagcaaaaga actgcataca gatactgtag agttttgggc atgtttagta
     8101 attaggttct tgttcaaaaa ttcgatgcaa gataatcttt ttttttatat tattgtatct
     8161 ttcgacttca atttaacgtt gccaatgatt aagcaaccga agacttgttt tgtaaatcga
     8221 agctaaaggc tgatgagatc cacaaaatta tatcagataa tcagcgagtt atctttaagt
     8281 gaagtgaaca gattcgaaaa atttgttaac tctccttatt ttaatattaa cattttaaat
     8341 atagaaatat taaaaatatg tattgaatta tataaagatg cggaattgca agtctcaaaa
     8401 atggatgtgt ggaatgatat caagccgaat gaagaatata acgatgtaaa gtttcggaaa
     8461 tacctgtcag acctgctcaa acttttattg gaattttttg ctgtggaaga acttcgaggg
     8521 cgatctatac tttcttctca actattgttg gagtatgcca ataaaaagaa gattcctaaa
     8581 ttaatcaata cttccgtcca ggttagtcag catacttcca acaaatttta caaccgaagt
     8641 agtgattatt atttgtatca ctttcagatt gagaaaaact tattttatct tcaggaaggg
     8701 aatattgagc tatatgcaaa aaagaatgta gagaatatcg cggagaatct ggataaattt
     8761 tacatcgctg agaaattgaa atattattgt gagattttgg ggcgggaagc attgtctaac
     8821 attcagtaca actttaattt catatctgaa cttctttctt ttgttgaaaa caatgatttt
     8881 gataatgatt tgattatcag aatatattat ttgatctata aaactctttc cacgcctgat
     8941 gagtcagagt attattacca gttaaaggct attttatttg aaaattatcg tttatttgat
     9001 ggttttgaac tacaggaatt atttggatcc attttgaatt actgtattgc tcaaatcaat
     9061 attggcaagc aagatttcca ggatgagatg attagcattt atgattttat ttttgaaaaa
     9121 aatattttgt tgaatgaaaa ctacctgacg cctgccaagt tcaagaatgt tgttaccact
     9181 gccttgcgag tcggaaatta tgactttgcg gaaagtttta tcaaggagta cgacaatctg
     9241 cttcctgtga cacaaaaaga aagtatagtc actttttcac ttgctcaaat atatttttat
     9301 aagaaggaat tcggtcaggt tattaaacaa ttgcaaaagg ttgaatatcc ggatcttatt
     9361 accaatctac gtgccaaaac aatctacctt gctaccttgc tggagttgaa ggaatatgat
     9421 gccatcagta tgtggtctga gagctttaag gcctttctaa atagaaagaa aaaagaaatt
     9481 cctgcagcac gcatcaagtc atatcagaat tttattacaa ttgttcggct aatcgccggg
     9541 gctgaatttg gtgacaagaa attttatcat agagtgataa aggaacttga taagttttca
     9601 ggaaatatag tcaacgaaca atggctcagg gaaaagttgt cagaaaggtt tagaatgtag
     9661 aaataggcca aatgactaag ctcgcacaga gaattgactg gtggaaagtg ttgaaatggc
     9721 ttgtattgcc ggtttttata gcagtactct actaccagtt gaccagtcta caacaaaaac
     9781 cggatctact aaatcgattg cgtgttttcc tggctgaaga aaacatcata attattgtgt
     9841 ttgtcttacc cctgcttaca ttttttaatt atgcattgga agctgctaaa tggcaggtac
     9901 tggtcaagcc tgcacaaaga cgactttatt cagaggcttt gaatgatgtt ttgataggta
     9961 ttttgttttc agttttaact ccggcaagac ttggcgagct tgccggtcgc gttgttaatc
    10021 tcagaatggg gaataagctt gtcggagcag cgtccagcct tgcgggaagc attggccaga
    10081 atatcgtgat ctttttttta ggatctgttg gctttgtgtt ttttgcgctg ataataggcc
    10141 acctgggaac atggccggca gttggatccc ttttcctcgt tttttgtttg accggattat
    10201 tccttttttt attctttaat ctttatctgc tcataccgat tgtaaataga gtttcattac
    10261 caaaaagatg gaagcaactt acagagaaac tgtatgcttt gaaagattat tcaaacacag
    10321 acctgctcaa agtcttatta ttttctttgt tgagagtggc aatatggtca tgtcagtatc
    10381 tgattatctt atggatgctg attcccgaca ttgactggca agtcgcactt ttagtttcat
    10441 ggatagtttt tttgattcag accggaatcc ctcttccgcc gatatcgagt cttgtagctc
    10501 gaagtagtat cgccatctat ttgtggagct atatcggaat ttcggaatgg aacgctttga
    10561 cagcaagctt tgttttgtat tttaataatc tgattatacc atcggtagta ggtttgatcg
    10621 ttatatattt aaacaaaaaa tcatttaatg cgaatattta gtttttttgc tacagcgctc
    10681 ttgtttattt taccattgaa aagtggatcc aaccaggttg atggctattc aaatattgca
    10741 atttcgacct gctcaggtga caatgtcaca ttcagggata gtgaagagtt gacctataaa
    10801 atatattata actggggact cgtgtggtta agcgcaggag aagcaacctt ccgtgtccag
    10861 gagcaaaaaa acgggtattt gatttcggcc gttggaaaga cctatcctgc ctatgaatgg
    10921 ttatactcgg tgaatgaccg atatgaggtt tttgtggata agtccacctt gcttccgatt
    10981 tggtctgttc gtgatatcag ggaaaacaat tatacccaat acgaaaggat ggacttcaat
    11041 caaagtaccg gtaaagtgac atccaggagg ggcaagaaca aatctgagac tacaacagct
    11101 gttatttcgg ttaatgattg tacccatgat attttgagta tcctttattt tgttcggaat
    11161 tatccattca ataccttcag gaacgggcaa gtattccctg taaatgtgtt tttggaccgt
    11221 gaaaacaata atctgactgc caaatataaa ggtattgtca aaaagcataa tgtacggggg
    11281 cttggcaact tcaattcttt tgttataact cccgccacta ttgaaggaaa agtatttgaa
    11341 aaaggcaaca caatgaaaat gtacatctca gaagatgaaa accaaatacc tttaatggtt
    11401 gagacaagtc tgaaggtagg aaatgtaaaa gtaattctca aatcctataa aaatctcaga
    11461 tattctctga aaagtaaagt taattgattc tgcctgacac acaactttat acgtttgctg
    11521 aaggaagttt tctgataata tttttgtgaa tactgacgta ataagataat tcagatggtg
    11581 aaatatttat tgggcattct gctgattcta ttcctttttt ttggtggata ttttttttct
    11641 gaattcctcc aaaataaaag ggaggagaaa acaaaagtgt ccaacgaagt aattttaaac
    11701 cagataaagg aagtggctaa attggtttca gttgaaggat actttactga aatctataag
    11761 tacaagaatt attataacct cgactggagt atttttacca aacgcgcatt ggttagggtt
    11821 aaagcaaaag tttcaatggg tattgatcta aacaaaatga agtttgattt cgatgagaca
    11881 agcaagacac taacaatagg ccctatgcct cagccggaat tgtttagtat tgatcatgat
    11941 atagattatt atgatatttc ggaaggtacc ttcaattcat tttcttcgga tgatttcaac
    12001 aagatcaata atgatgtaaa gcaatatatc cggaatgtgg ccttaaaaag cgaattacct
    12061 cagagggcca aactgcaaag tcaaaagaca ttggaaacaa ttaggattct ggctgagacc
    12121 gccggctgga aagtcataga ggaaggtatt gcgccggaaa agggttgaaa caatcaatgg
    12181 atatggacaa gtgtaatgcc gtcaccgcca gcatcaggag caggatggct gatttgcatc
    12241 ggtaggttgt attctttcaa tttttgtttg acaatcctct tcaatgtacc atcaccttta
    12301 ccgtgcagaa tttcaaggtt gatcttaccg gcaatcaatg cctggtcaaa gaattcctga
    12361 agctgctgca ttgcctcatc tacacgtaat cctctgatat ctatcttgtc ttttacctta
    12421 ctgacatcac taagtatatc ggtgaataca ccctttactc ttcttaaatc ctgtggcttc
    12481 ctttctgcta taagctgaga gataggaatc aggacagtta gtccaccaac acttacctta
    12541 gctttgtcct tgagaatttc ttcaacggtt ccggtagtgt caccagattt taacctgaca
    12601 aaatcaccgg tttcaatcgg cttattatgc attgctgttt tgcttatacg aagtgtttca
    12661 ttgtgcaaac tattcacttc cgccacaagc ctttctcttt cagcacgttt ttttagtgct
    12721 aattctctga tctgatccaa atctttttcc ttcgataact ctctgatctg gtcttcaatg
    12781 aattcatttt gggcattaat actcttaagt tggaattcct ttacctcttg tctgaatttt
    12841 tttcgtttaa attcatattc ggccagctga cgctcgtaat tggcaatcaa tttatccaac
    12901 tgttgttgtt ttttttcaag gtcagtgatt tgcttattca atttgttttt atctgactgc
    12961 aggtcattca ataaacggtc aaccttatat tttttggatc caagcttttt ttccgcatga
    13021 tgaagaacta cagcgggaag accattactg gttgccaatt caaatgcaaa tgaacttccg
    13081 ggacttccca ttttaaagac ataggtgggt ttaaggccat ttgaatcaaa cagcatggag
    13141 ccattggtga cacccgaatt gttatgtgcg aataatttga gggtagagta gtgggttgtt
    13201 attactccca tgcatttttt tcgattgagg aaggctagta cactttctgc aatagcacca
    13261 cctacgtccg gatcagtacc tgttccaaat tcatcaatga gaatcaaggt ttctgaattg
    13321 gtctgatcag tgaagaattt catgcttttc aaatgactgc tataggtact cagatcttct
    13381 tcaagtgatt gcttgtcacc gatgtcagca aaaatatttt taaaaatgcc aagcttactt
    13441 tctttatata caggtaacaa aaatccacat tgtgccatga cctgaatcag accaacagct
    13501 ttcatcagga ttgattttcc acctgcattt ggcccggaaa tcaacaggat tctgttatta
    13561 tctgacatat ttaagtcaaa agggatcgtt tttttacctg ttttatgatt cttaatatat
    13621 aaaagagggt ggaaggcctt tataaaattt aaatttggat gtgagacgat ttggggtttg
    13681 tcaccaccat acaatttggc taattgagcc ttggcaccta caaaatccaa atatgctaca
    13741 caatcgaggt atcgtacgaa gtcgtcatat cctgttgcaa gattattaca taatttttta
    13801 atgatcctaa caatctccct gagttcttca tgctgaagtt caaataaatc attattcaat
    13861 aaaatgacgg gttcaggttc aatatatacc gttttcccgg tttctgattc gtcatgtatg
    13921 attcccggaa tcttccgctt atgttcggcg gggacactca acacccttct gccgttgcgg
    13981 taactttcag acgattcgga aagaagccct tttgacttgt attgtgctgc tatctgatcg
    14041 aaagccctct ctagttcgga ttttttagct gaaatggctc tccttatctt tacaagatct
    14101 tcagatgcat tattcttgac tttaccttga tcatcgagta tttgattgat ttgctggaga
    14161 taggtaggaa tatatccttg atcaaggatt ttttgagtaa gtacgggata gtttttggta
    14221 ttgccggaag aggaagcgaa tctgtcgagg tttttcatta tttccaaaac agatcttagt
    14281 tcattgatat gttcaacttc caggaaataa tcggtaattt taaggtattt gaggaaggta
    14341 tcaatgtcgg tgtaattacc cgttggaaag ctatcgcctg catccaatga taacttaaat
    14401 tccattgttt cactcaacaa tcgttctatt tcatcgggat tagtatgaaa tcggacattc
    14461 ttcaacaacc gtttaccggc ttctccttta cagtaagatt ctgccaaaat gagaaggcgg
    14521 tcaaattcca atacctgaaa aatatcgttc ggataaaaca caatgtaaca gatttgagca
    14581 aacaacaaaa tgccccgcga gttggttcac gaggcatttt atttttattc ttttattgaa
    14641 taaataactt gtcgctaatc agaattctgt tgtatttgtc cctgacggtt gcaatgtaga
    14701 ggcctggtgt cagccctgca gataagggga tggtattttc attccctgaa acttgcctat
    14761 cataatatgt cattcctaac atatctgtca aagaaaaatg atattcattg ttagttgaag
    14821 gattgatacc gatgaaactg aatgaaactt catccctggc aggattagga taaattctgc
    14881 cagatgagac ggggatttca ttcttggaca gcaaagaagg gtctatattt ccggtggtgt
    14941 actctcctgc taagaaattg cttataagga aagatccttt tttgtcactt tgcgaacctc
    15001 ccttcttcga ataatatgga tagatttgcc attcttctac tccggaaggg cgatagaata
    15061 gttgaatact atcctcacca ttttgtaaaa gatcactatc ccagtttaga ttatattcta
    15121 ttttaccggt aaatgaaaca tgatcagggt cagctaccgt aaacttccag aaatgtttgt
    15181 tattgaccct gatagcagga tttgttccgg catcaggtgc tgtatagtgg ttttctaaga
    15241 aggcataggt tgaatccttg acttctgccg cgcttatgct caggttggaa tatgtaaagg
    15301 taacactccc ggtctttttg acattttgga gggaactgaa ttgagcaaga ttcagagcca
    15361 taggataatt aagaaatact gccttaggct ggaaagccag attcaatagt atttcgcttt
    15421 cttcacctga tgtagttact ctttgattaa ccaaattatt atcctgatca acaaaagtca
    15481 aaacaacagg tgcatcttta caaaacccat ttgatttaag cagtttttgt ttaattttta
    15541 ttttatgcgt atagctggaa aaatctattt cttgaaagtc agaaataacg aaatcgtaaa
    15601 atcctatatc gaagacccat ctattaaaga agtcagtcaa atctacacct gaagcagcgc
    15661 ttaaagcatc gcgatagcta tatgccgtca gattggaata tgcataatct ttcagaactt
    15721 tttgttgtgc tgttctgaaa aggtcgtctc ccagataacc tctcagtgaa tgcataacca
    15781 ttgcaccttt cttataagtt gtggctccat aagtaatatc cttgggcatc ggtgaaagag
    15841 gaaggtagcg tccatcactg atatgggcgg tagtgataac atcatatttg ttttcactga
    15901 caaggcgttc gaaggattgc ctgccattta aatattcgtc aaaaagatgg gcgccatatt
    15961 ctgcattacc ttctttgatc cacatatcgg cttgtgttct tagtgtaagt aaatctcccc
    16021 accacagatg acacaattcg tggctaaaca ggcgtctgtc agcggcattc cctgatgtac
    16081 tggatacagg ataagcgata cagttgggat gttccattgc acctcttgtt gtcataacat
    16141 agcctacacg ttcgaaaggg tagggaccat accaggactc cagtgcatcg attgctgtac
    16201 ctaaagtact gaaagcattg ataactctgg cttggtcaga aggtttactt accagttgga
    16261 caggtacttc accataaata ccgtcgtgat tgtattcata ggatacgtaa gtagaggctg
    16321 caatcgctga aagataggtc ggtatttgct gatccattct aaataccctt attttggagt
    16381 tgtcattgaa tggttgttca tcgatcaatc ttcccacgct atatgaggtg atattgttgt
    16441 ttgtaatgat tctgtatgta taggtactcc tttcgacaaa attatcgaag cagggaaacc
    16501 agcttctacc gaagttgtga ggatcatccg ttatgccaat tccgaggtta tatgcgattc
    16561 cgtcctcaaa atagaatcca ccgaaaccag aagtggacgt cttaggcgtt ccgtgataat
    16621 gtacagttac attgtaggat ttgcctgctt ccaattggta acctgcataa atgttcaata
    16681 aaattctgtt gtaatcatag gtggcaggaa caccttcaaa aaatactgaa tcaacatcaa
    16741 gttttagtaa gtcgagtggg atatagtcca atcctaccat cttaggagta aatgtcacaa
    16801 tggctttgcc tgtcaaaata ttctgaccca tctgacgcag gtctatcatc aggtcgtaat
    16861 caaggatatc aaatgtatcg gatcgttcta ccgatgcggc ccacagacta ttttcttcta
    16921 ccgtgtttac cctttggttg gccagtttat gcctcatatg acagccatac atttcaagag
    16981 gctgggatat gccggaaaaa cttattatat taaatacaat aataaatagt ataattctca
    17041 tagctatata attgatcaaa ggatagtagt cttcactgca tcaagcatat ttctggtgtc
    17101 cgcaacatct ttaccttgtg catacacacg aagtaccggc tcggttccgc ttgggcgaat
    17161 catcacccat ctttcatcgc caaaaatata tttaaatcca tccaggtctt cggttgaaat
    17221 taccttgttg ggtccgaaag aagtgtattg gccttccttg caagctttga tcacgttttg
    17281 tttctgttgt tccgaaatat ggagatcatc cctatcaaac cagaaagcac caacctcttc
    17341 atagagttct tggattaatg cttccaatga cttaccggta gaagccatga attccaggat
    17401 catcagacca atccatatac cgtctctttc gggaatatgt ccttttacgg ctaatccacc
    17461 actttcttca ccacctacaa gtacatcacg tgtggtcatg atctcagcga tatatttgaa
    17521 cccgattttt gtaatttcac atggcaatcc gtatttattg gccagttcac gcattttatc
    17581 ggttaccgag aaagtaataa ccacttcccc tgtaagtttt ttgtatttgt attgataaag
    17641 aagaaggaga agaagaatgt gatgcgcatc aacaaatttt cctttactat caaacatacc
    17701 tattcggtct gcatcgccat cattcgcaag accgattgag atatcatctt tggagctgat
    17761 gagctcagaa agttctttca gatttttggc aattggctca ggagcttgac ctctgaagga
    17821 agggttatcg tcacagtgta gtgccataac gtccggtaga agtctaagca tagctctttg
    17881 tcctgcaccg tacatcgcat cgtatgcaat tttaagacca ctatttctaa ttagatcaag
    17941 gtcaaaacaa gattctgcat gattgacata gatatcttct aaaggcactt cttgtgccat
    18001 attttgttga acataatctt caaatgtggc agttgttgtg ttccattcat ccggaatgag
    18061 attttcaacg gaagtgatat cagcaggaga ggatggccct ccgaaatgtg attttaattt
    18121 gtatccattg tatgatggtg gattatgact agcggtgatt acaatgccaa gatcagcctt
    18181 aagtttcacc acacccaacg agaccatagg ggttgagaca aatcctttgc catataaaac
    18241 cttgatacca tgaaaacaaa atacttccgt aattgcttta aggaacatct cgcctccaaa
    18301 tctgcagtcg tgtcctacca caactgtttt catgcctgat tgcttcatcc aggcagctgt
    18361 tccggcggcc acacgctgca cattatcgac tgtatattcc ctggcaataa tagcacgcca
    18421 tccgtcagtt ccaaacttga tctttatcat aacttctaat tttttgcctc taatataatt
    18481 aaaattgtat gtagtggagt ttgaaatagt aactactttt tgatttttat taatctagtt
    18541 gcctcataca ttacttattg tgtatcggcc ggattgtcca gatccgactc ttcgtatgaa
    18601 atcaatttga gcattgtgaa tgcggaaaaa ttgaatgatg gcatagaata aaaaaagcct
    18661 gtaaggcaat acaggctttt ttatattcaa taaatagttt tattccttaa ataattcttt
    18721 taattgaata aaatcggctt gaccaatatg cagatgagtt aatagcgtct attgttacac
    18781 ctttactatt gctgctatgg attattttta aatcattgtt gtaatttgaa taaacgattg
    18841 cgacatgatg gactctacct cctgctgcaa aaaagaccag atcgccgggt cttgcttcct
    18901 ctttctttac acttacccct attgtagata aggttctgga gctatgtgcc aagtcatatc
    18961 catattcgtt gaatacatag ccaacaaatc cggaacaatc aaatcctttt ggagatgtac
    19021 ctcctgaccg atagggagtg ccgaggaatt tactggcaaa gtcaactaca tttgctctga
    19081 tcttaacttt agctgagaca ttgaacatca tattaagcgt gttcaacaga aatggtggga
    19141 tagaagcttc atttgtaacg tttcttgcat tagaaactga agggatggtc gccaataaaa
    19201 acaatgccac tattgactta agcaggatag acttcatcta ttcgcagtct ttaggtttag
    19261 taataagttt ctttttcgag cttactaaaa aagcccggat aacatgaaga ttctaatgta
    19321 tatgttcttg tttgtaaata ataaaattag atacaaacgg gactaattga tttcacaaag
    19381 atagtgcaac atatctgtaa aacaaatata agagagaact ttcagtatat actattgata
    19441 tattaatatt tatgcataaa gtaacatcgt gtaaccattg ccagacatat cacattattc
    19501 aatattataa tatataatca aaaaaacaaa taaaatgtta agataatctt aagatggaat
    19561 aacaaaattg gaaggatgta gaaacaaaga attacatatt tggaatgtcc tagagtctat
    19621 tttactttgg cactaatatg taagcataga ttaaatgtgt tagggtaata ttttagttta
    19681 atttggatca aaaaaaaagg ctctcgtaga tgagagcctt ttgctatgaa agaaattata
    19741 ttcaatatca atatgaccaa aggtcgtgtt caaaattaaa tatttcttct ttgatcttat
    19801 tagattcaag aagcatatca actccatttt cccttaaatt atcgtaatca accaaacgga
    19861 aatcatttac attggatttc tttgtgataa aactggaaaa atgacgattt tcaaacatat
    19921 catcccaaga aacagggctg gcaatgttag cttcattaaa cacttggtgt tgggatagat
    19981 attttctaca atcgggataa taaatccaga ataaaggcat agcaggaagt gcaacccctg
    20041 tagtctggtc tattttgaca tacaatgggg ctattcccaa tatccttact ttcatggtgg
    20101 aggactcaga atcaaaatac caaacttctt ttacccgaaa ttggttaata agctgaggat
    20161 cgaaatcatt ttttgtgata accgactcat attggccggt ttcagggttg tatacttcag
    20221 aagtatcaat tccggaaaca gtcttattta tgtcttcggt agaagctctg aattggaatg
    20281 actcatcagt ataacaacga atatccccat tttcaattcc ttttttcagt atggtataga
    20341 actcatggtc aggatatttg aaaggcagat tcattttctc tctacaatca attactctcc
    20401 atattcttct ttcccaaaaa atatccctct catttaaagg ttcataagcc aatacaagtt
    20461 tttgcttaat catatccctt ttaatgatat cgtccattgg agcatcaggg tccggttttc
    20521 tgacagtatc ctgtgctgca actatcaccg gagaactagt tttgggtcct gttgtcttcg
    20581 gagtagttga cttagtggaa ctgtttgttg cgct
//
LOCUS       DAOQQN010000008        20116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_798_length_20116_cov_7.388465, whole genome shotgun sequence.
ACCESSION   DAOQQN010000008 DAOQQN010000000
VERSION     DAOQQN010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 20116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20116
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_798_length_20116_cov_7.388465"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(596..1588)
                     /locus_tag="PLG24_00650"
     CDS             complement(596..1588)
                     /locus_tag="PLG24_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23417.1"
                     /translation="MASRSQTEIMHLLSSGLPKHELVTALQSLLGISKPTAYKYLSQD
                     VSIAYDHMVKILEYPPFQRVLYQGGETGQSNIVWNYPVFDRPVTDALAYITSIHNFIT
                     LFSGTEVEIKYTTYEIPLFYYLYYPRLFAFKLFCWSKTIWKTPGFENVKFNYEAIMTP
                     EVRTVAEAALQRFSELRSTEYWTDQLYDNTIRQLQYFRDMEYFDNMKDVRNIARDIHS
                     LIGLMKVMASSGKKYNASKSGFAADITIYRNDIYFTNNIVSVHAPKQIITFCAFDNPN
                     FIITLDPKLGMRTAEWLENMQSGCTRLTQNNDLVRLNFFRTMEEKLSVLGFNFE"
     gene            complement(2346..3020)
                     /gene="ung"
                     /locus_tag="PLG24_00655"
     CDS             complement(2346..3020)
                     /gene="ung"
                     /locus_tag="PLG24_00655"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HMZ23418.1"
                     /translation="MHVKIQQDWYNLLEPEFQKPYFSRLTTSIKEKIAAGVTVYPPGK
                     LIFNAFELTPPQQVKAVILGQDPYHNPGEAMGLCFSVPRGVSVPPSLRNIFKELKTDL
                     GVDPPNHGDLTTWARNGVLLLNSILTVEKNSAGSHQKLGWQEFTDAVIRNISDYSKCV
                     VFILWGAYARTKVPLIDADKHLIITAAHPSPLARGAFFGHRPFSHTNNYLIENNITPI
                     DWAISD"
     gene            3067..3393
                     /locus_tag="PLG24_00660"
     CDS             3067..3393
                     /locus_tag="PLG24_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23419.1"
                     /translation="MKYIFLTLTLVISCIPAEAQFRPVNSGMDSIAISDLYFVILTDS
                     GKDAVRIGVGTWKEQYTTTPFFCTLENKVNKRSVRPVFFRLGNKDYTDMLERKDINKA
                     ITGPLH"
     gene            complement(3875..4768)
                     /gene="atpG"
                     /locus_tag="PLG24_00665"
     CDS             complement(3875..4768)
                     /gene="atpG"
                     /locus_tag="PLG24_00665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HMZ23420.1"
                     /translation="MGANLKEVRERIKSVISTQQITKAMKMVSAAKLRRAQQAIVQLR
                     PYSEKLNEMLSNILSNLDGDASSVYNAEREIKHVAIVIITSNRGLAGALNTNIGKEAT
                     ALARTKYAEQLDANKVTFITIGKKGYDYVKRVHKGAPVITDYINAFSDLSFSNIAPIA
                     STLMNDFATGKYDAIEISYSKFKNAGTQLFSAEQFLPVLKKPLSAMRGNMKADYIFEP
                     DQKSLLEYLVPTILQTQFHKTLLDTNASEHGARMTAMDKATDNADTLLKELRINYNKA
                     RQEAITNELSEIVGGAAALNG"
     gene            complement(4847..6433)
                     /gene="atpA"
                     /locus_tag="PLG24_00670"
     CDS             complement(4847..6433)
                     /gene="atpA"
                     /locus_tag="PLG24_00670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HMZ23421.1"
                     /translation="MVNVKPDEISAILKQQLSGFHTEAELEEVGVVLSVGDGIARVYG
                     LTNAKAGELVEFETGVQAIVLNLEEDNVGVVLLGPSDDLKEGSKVKRTNTIASIQVGE
                     GYLGRVVNPLGQPIDGKGEITGDKYEMPLERKAPGVIFRQPVTEPLQTGIKAIDAMIP
                     IGRGQRELIIGDRQIGKTAIAIDTIINQKEFYQQGEPVYCIYVACGQKSSTVAQVARV
                     LEENGAMAYTTIVSASAADPAPLQFYAPFAGAAIGEFFRDTGRPALIIYDDLSKQAVA
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKVISNDAIAQNMNDLPESLKKSGLV
                     RGGGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLESNLFNSGVRPAINVGISVSR
                     VGGNAQIKSMKKVSGTLKLDQAQYRELEAFAKFGSDLDAATKSVLDKGVRNVEVLKQA
                     QYSPVSVEKQVAIIYLGTNNLLKDIKPSDVREFEENLFAALDKNHPEMLANFKAGKLE
                     DADLETLRSVATTVSKNFAR"
     gene            complement(6463..7005)
                     /gene="atpH"
                     /locus_tag="PLG24_00675"
     CDS             complement(6463..7005)
                     /gene="atpH"
                     /locus_tag="PLG24_00675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HMZ23422.1"
                     /translation="MSVSQVASRYAKSLLDLAIDQKKTEAVRGDMAVFKEALKSRDLV
                     LLLKSPIIFPDKKIAALRAVFEGKLDTLTMSFFDLTIKKGRESLLVDIADQFTEQYNT
                     LFNIALVKVTSAAPLDEATTNIILDKIKGLVGSDKQIQLETALNPDLIGGFIIQYADR
                     LYDSSVAYQLNKLKQTLTHN"
     gene            complement(7030..7542)
                     /gene="atpF"
                     /locus_tag="PLG24_00680"
     CDS             complement(7030..7542)
                     /gene="atpF"
                     /locus_tag="PLG24_00680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HMZ23423.1"
                     /translation="MVLLAEFNVIKPEFGLFFWTVLIFLSLWFFLGKKAFTPIANALK
                     EREDSIKDALAQADIARNEMNNLKAENEQLIAQAREEKAAMLKEAKENAAQIVSDAKS
                     KAKEEAGRILANANLEIENQKKAAISELKGQVGLLAVQVAESVIKKQLSNDPSQIELA
                     KSLANEIKMN"
     gene            complement(7625..7825)
                     /gene="atpE"
                     /locus_tag="PLG24_00685"
     CDS             complement(7625..7825)
                     /gene="atpE"
                     /locus_tag="PLG24_00685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF005363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HMZ23424.1"
                     /translation="MYAGIGAGLAAIGAGIGIGFIGGKGMEAIARQPEAVGDIRNNML
                     IIAAFVEAVALFAVVVAFLTAG"
     gene            complement(7906..9144)
                     /gene="atpB"
                     /locus_tag="PLG24_00690"
     CDS             complement(7906..9144)
                     /gene="atpB"
                     /locus_tag="PLG24_00690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HMZ23425.1"
                     /translation="MTKLYSSLNIRFSVFPRLYFLVLFTILVTPVVNFAQDHSHDAPA
                     GEVHDVVEGVLKEEEEFKMNDLIFNHINNSNDFHIFGHIAVPLPCILYSKEDGWTMFM
                     SSKLEHGHKAYNRYVLDEGIVKRLPADFPDMGHVEIGHIKHTKEGSVEVATLEHQGKE
                     YTLEKASAFTGMTSFYDFSISKVVFSMLLAAILLVILFLSISKAYRNRSGQAPRGLQN
                     LIEIIVLFIIDEVAKPMLGDRYQKHLPFILTIFFFILVCNLMGLIPFFPFSANITGNI
                     AVTMALAVMTFLVTNILGNGNYWKHIFWMPGVPPAMKLFLAPIEFLGVFIKPVSLMIR
                     LFANITAGHIIILSLIGLIFVFGKVGASMGGAIAGTALAIPFTLFLNVIEFIVAFIQA
                     FIFAILSASYIGAATEEAHH"
     gene            complement(9968..10903)
                     /locus_tag="PLG24_00695"
     CDS             complement(9968..10903)
                     /locus_tag="PLG24_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23426.1"
                     /translation="MRRNLYPPISHRPIRTDSDLSEVSSGRPFSASIKNMQVPPEFHL
                     TSSGTQKIVQMKKAGSGDTKAEAPDDKPDIYSEKYPNLDSLPVFVDREKGEKFTYSLV
                     AGKFSYAQSVLRSWLEEMAGLGADKSLLEELSLGVEDLDAYLFDFKNKKGALDEDDLI
                     YAEELSKLIIELGSTLSPKIEAERSKKKKPAPKKVKPQSSGKQILFGKASMETMKSIK
                     KVEEIGSLLEKTLVIGTTINTATSLADPAEGLYMILTLDDPFTPVAQALSDIDQIKRY
                     RIIEEISYLLALHSKELNVEERQYWEKLLASFRKK"
     gene            11594..11932
                     /gene="ssb"
                     /locus_tag="PLG24_00700"
     CDS             11594..11932
                     /gene="ssb"
                     /locus_tag="PLG24_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HMZ23427.1"
                     /translation="MTTLSNQVQLIGNVGQQPEIKTFDNGGKIARVSLATNEKRKNAT
                     GEYITETTWHNLVFRGKHAEIVEKHVEKGKEILVEGKIINRSYEDKNGDKRYITEIQV
                     LNFKFLGGRN"
     gene            12149..12442
                     /locus_tag="PLG24_00705"
     CDS             12149..12442
                     /locus_tag="PLG24_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23428.1"
                     /translation="MSSGPIVIWLSNFLCYGFFALVCRCLVIGLQTERAHYELNLFQN
                     PGKYGKRPAPSDESINPAILLIQTTRSMEAWRIKTTGVRYGLALFYPGGIGCH"
     gene            complement(12886..12959)
                     /locus_tag="PLG24_00710"
     tRNA            complement(12886..12959)
                     /locus_tag="PLG24_00710"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12923..12925),aa:Met,seq:cat)
     gene            complement(13019..13267)
                     /gene="atpC"
                     /locus_tag="PLG24_00715"
     CDS             complement(13019..13267)
                     /gene="atpC"
                     /locus_tag="PLG24_00715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HMZ23429.1"
                     /translation="MQLSVLTPDVEYFNGEITSVKVPGVSGEFQILNNHAPIVSALAE
                     GKVEVKTAEGKLMSFKIQGGFIEVLNNNISLLVQSITE"
     gene            complement(13301..14818)
                     /gene="atpD"
                     /locus_tag="PLG24_00720"
     CDS             complement(13301..14818)
                     /gene="atpD"
                     /locus_tag="PLG24_00720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HMZ23430.1"
                     /translation="MANIGRVKQIIGPVVDVSFSGEGSKLPDIFNALVIKREGTNDLI
                     LEVQQHLGEDSVRTVAMDATDGLQRGTEVIDTGGTISMPVGDHIKGRLFNVVGDAIDG
                     IGEVEKGSNAYSIHQKPPTFEQLSTETEILYTGIKVIDLVEPYQKGGKIGLFGGAGVG
                     KTVLIQELINNIAKGYDGMSVFAGVGERTREGNDLLREMIESGIVKYGDEFKHSMEEG
                     GWDLSKVDKELLKNSQATFVFGQMNEPPGARARVALSGLTFAEYFRDGDLNDPNGGRD
                     ILFFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGLMQERITSTKRGSITSVQ
                     AVYVPADDLTDPAPATTFAHLDATTVLDRKIASLGIYPAVDPLNSTSRILDPNVIGHE
                     HYNCAQRVKGILQRYNELQDIIAILGMEELSEDDKLVVHRARRVQRFLSQPFHVAEQF
                     TGLKGVLVPIEETIRGFNMIMDGEVDEYPENAFNLVGTIDDAIAKGKKLLAEANA"
     gene            complement(14997..15686)
                     /locus_tag="PLG24_00725"
     CDS             complement(14997..15686)
                     /locus_tag="PLG24_00725"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HMZ23431.1"
                     /translation="MTPEIWISELQSQLAGHRNQANAVHIIRYLKGQFGSFGIKQGER
                     RLIVKPFIKNARIFDTQALTILLELLWQNEEREFQYTGMELLDTYRKKFGKDIKKPLE
                     QALTEKSWWDTVDFLATHTAGRSYQDGMLTTDDLYAWNSSGNIWLIRTSILTQLKYKS
                     STDWEMLQQFILPHLLHKDFFIRKGIGWALREYAKTEPARVRSFVEHHEMSGLSRREA
                     LKNLYPGGTKR"
     gene            complement(15676..16113)
                     /locus_tag="PLG24_00730"
     CDS             complement(15676..16113)
                     /locus_tag="PLG24_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HMZ23432.1"
                     /translation="MLAINLERARKYCAYQERCHSEVRSKLIEWKIFGEELEQIIATL
                     ISENFLNEERYARAFVSGKFNINHWGRQKITYALKQRQVSDYSIRKGLEVIDEEEYQQ
                     LIRRLLERRMKEKKTQSPYQLTQYLMSRGFEYNEIEKVLSHDA"
     gene            16277..16708
                     /gene="dut"
                     /locus_tag="PLG24_00735"
     CDS             16277..16708
                     /gene="dut"
                     /locus_tag="PLG24_00735"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HMZ23433.1"
                     /translation="MQVKVINKSRYSLPQYETAGSAGMDLRANTDMTLELKPLERALI
                     PTGLYIELQPGTEAQIRPRSGLSIKKGLTVINAPGTIDSDYRGEIKIPMINLSDELQT
                     IDPGERVAQMVVAKYETISWMEVEDISPTERGSGGFGSTGG"
     gene            complement(16842..17810)
                     /locus_tag="PLG24_00740"
     CDS             complement(16842..17810)
                     /locus_tag="PLG24_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23434.1"
                     /translation="MGLLSWIFKACMSPPTEEKRFANDAKIDTFEVPIYHEKEVDYTY
                     YKKYIDSTKILPFNYRDDAVFFMTMDDENLYVSMKPSVGLLKSAPEEAELRKPYLGLT
                     AFDIKTRKMKWHLHYDDPAFTPFKVNDATIQLKVPTLIDGKLIQLKSSFGDPQLGCYS
                     IIEPKTGKIIKTEEITNGGYIIYDLKYLLQVNHTNKVSRLSTEDGSVLWTYNYPLPQP
                     ADPNGLNLIGQKLFANRYLKDGLVNDDIIDAETGKVLYTTKIKETDLGKTKLNLVACT
                     DDKAYFSAVVHWGMDRKMASKQVRYNIEYDIANQKVLRRSEYDVEK"
     gene            complement(17898..18580)
                     /locus_tag="PLG24_00745"
     CDS             complement(join(17898..18253,18253..18580))
                     /locus_tag="PLG24_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611661.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HMZ23435.1"
                     /translation="MNCPKCKSAQHCKDGIVKGRQRYLCKECGMHYTVEKKSDVKTAE
                     VRRMALEMYLEGLGFRAIGRILKISYGTVYRWIKEWGSKVSLPQNSQPIAVVEIDELH
                     SYVGSKKNYCWVWIAVDRFARRFISFVCGSRGTETGTKLYNNLKEIKVGFYCMDHWKA
                     YEEILASEFHIRSKAETYSVEGYNSRIRHYLARFKRKGKCYSKAQHMIEKSLNLLFLK
                     LNNELPILI"
     gene            complement(18638..>20116)
                     /locus_tag="PLG24_00750"
     CDS             complement(18638..>20116)
                     /locus_tag="PLG24_00750"
                     /inference="COORDINATES: protein motif:HMM:NF023433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23436.1"
                     /translation="SVDAAKCYYEELNNLQKDASTSSATNYKPLKIATIFSFAPNEEQ
                     SAIGDIVDETFEPTAMDVSAKEFLTKAISDYNAMFKMNFNVEGKFQNYYRDLAKRVKN
                     KEIDLIIVVGMFLTGFDAPSLNTLFVDKNLRYHGLIQAFSRTNRIYPNKAFGNIVTFR
                     DLEKATIDAITLFGDSNTKNVVLEKSYKEYLEGFTDIVTGDARRGYNEIVNELKTKFP
                     NPDEIVKEGDKKEFVKLFGEYLRVENILQNYDEFTHLKAFQAIDKNDPEAIEEFKKAH
                     FITDEDIAAMRNIELLKDRNVQDYRSTYNDIRDWLRRERSGREAEKSTIDWDDVVFEV
                     DLLKSQEINLDYILELIFQHNKKTKDKDELIAEIRRIIRASVGNRAKESLIVDFIHET
                     DLDTLQDKANVIDSFLQFAQARQKAEALELITEENLNIEEAKRYITTSLKRKYASENG
                     TELNALLPKMSPLNPQYLTKKQSVFQKLVSFVEKFKEVGGQL"
BASE COUNT         5646 a         4349 c         4105 g         6016 t
ORIGIN      
        1 atcaaaaatc aaattctaag tattcaaaag taaggacagg aagacacggg acaaaataac
       61 aatgttccca accgaagtag gtagataaat aataaccgaa gataaagccc catggtcaat
      121 ccggaagaca tgcgattaaa aacgagtcaa aaaaagaaat aaatcgggaa aggaatgccg
      181 gaaacgctta cccctgttgg cagggaggct attaaccaag tacatagttc gatttttcga
      241 tatgcaggtt gtacccctcc aacacaaagt atcaaaaatc aaattctaag tattcaaaag
      301 taaggacagg aagacacggg acaaaataac aatgttccca accgaagtag gtagataaat
      361 aataaccgaa gataaagccc catggtcaat ccggaagaca tgcgattaaa aacgagtcga
      421 aaaaagaaat aaatcgggaa aggaatgccg gaaacgcttg tccctgttgg cagggaagct
      481 attaaccaag tacatagttc gatttataac atcttatttc ccactccgct tttcatgaaa
      541 atgtcaccca aaatcaaaat ctttacgtgc cactaaaacc cttctaatgt ctcagctatt
      601 caaaattaaa cccaagtaca ctcaattttt cctccatggt cctgaagaaa tttaaccgca
      661 ccaaatcgtt gttttgggtc aatcgtgtac atccggactg catattctcc agccattcgg
      721 ccgtcctcat accaagcttg ggatcgagtg tgatgatgaa gttgggatta tcaaatgcgc
      781 agaatgtgat gatctgcttg ggtgcatgga cggaaacaat gttgttggta aaatagatat
      841 cattgcggta aatagtaatg tcagcagcaa aaccggattt tgaagcatta tatttcttac
      901 cggaggaggc cattaccttc atcaggccga tcaggctgtg aatatcccga gcgatattcc
      961 tgacgtcctt catattgtca aaatattcca tatccctgaa atattgtaac tgccgaatcg
     1021 tgttgtcata aagctggtcc gtccagtatt ccgtagaccg cagctctgaa aaccgctgta
     1081 aagcagcctc agcgactgtc cggacttccg gggtcatgat ggcttcatag ttgaacttaa
     1141 cattttcaaa gccgggtgtc ttccagatgg tcttagacca gcaaaataat ttaaaggcga
     1201 atagtcgggg ataatacaga taataaaaca ggggtatttc atacgtggtg tatttgattt
     1261 ctacttcggt gcctgagaag agcgtgatga aattatgaat cgaagtgata taggccaagg
     1321 catccgttac cggccggtcg aacacaggat aattccagac aatgttactt tgaccggttt
     1381 ctcctccttg atagagcaca cgctgaaaag gcggatattc cagaattttg accatatggt
     1441 cataggcgat ggaaacatcc tgcgaaagat atttataagc agttggcttt gaaataccaa
     1501 gcaatgattg aagtgctgta acaagctcgt gcttcggtaa gccggaactt aataagtgca
     1561 tgatttcagt ctgagagcga gaggccatga ctatgttgct ttattgaggt attccaacaa
     1621 atatacagaa cgaatgactt tggatttcat ttattttaga ttaaaaatga acgcaatgcg
     1681 atttattgat acgaatttaa cataaggcta acaaaatcaa aagaatatat attttattta
     1741 ttttttaagc cgtggggttt tatatgatta tttattacta aaataaatat atgtataaaa
     1801 ttttatgaaa aaagttcagg tagaccgcca tagttaaaaa tcgtagaatg gtggatttgt
     1861 gcaggtgagg tgccgatacg aactttgtag catcagaaag ttacagaaaa gggccctatg
     1921 aaagtccaac ttcaaaacca atgaatcgcc ggaacccaac tgaaaggttt aacgcccaac
     1981 tcaaaagaaa ataattcgga tacacccgat tttaagtgaa acgtctctat ggttcagtaa
     2041 ttttgagtga gccaagttct acacaagggg atttagccga gaggatgaat ttttgattag
     2101 agaataggtt acgatgacag cctattctct ttttttttgg gcggatccgg aaaagaagta
     2161 tagcaatcag gcacttttta ccaaaaagat acctatacag gagagaacct acaggcatag
     2221 aatgaggaaa gcaatacctt tatatagaac aaattttcta tttagatcaa caattgaaaa
     2281 cctcctgatt cgtttttaga tccggagaat ggttgtacat cgatcaaata gaagtataga
     2341 gcatcttagt cactgatggc ccaatctatc ggcgtgatgt tattttctat gagataattg
     2401 ttggtatgtg agaagggccg atggccgaaa aaagcacccc tggccagagg agaaggatgt
     2461 gcggctgtta ttataagatg cttgtcagca tcgattaatg gcaccttagt tctggcgtag
     2521 gctccccata gtataaagac aacacacttc gagtagtcag atatgtttcg gataacagca
     2581 tcggtaaatt cctgccagcc gagtttttga tgtgatccgg cactgttttt ctccactgtc
     2641 aggatactgt tgagcaacag cactccattc ctggcccagg tggtaagatc accgtgattg
     2701 ggaggatcga caccgaggtc tgtttttaac tccttgaata tgttgcgcaa ggagggcggg
     2761 acagagactc cgcgcggaac ggagaaacac aatcccattg cctcacccgg attatgataa
     2821 ggatcctggc cgaggattac tgctttgacc tgttgtggtg gcgtcaattc aaatgcgttg
     2881 aatatcagtt tgccgggtgg atatacggtt accccggctg ctattttttc cttaatggat
     2941 gttgtcagac gggagaaata cggcttttga aattccggtt ctagcagatt ataccagtcc
     3001 tgttgtatct ttacatgcat aatatgaaaa tatccaaagg ctaaattaat tcaaaccgga
     3061 aatccaatga aatatatttt tctgactctt actctggtta tatcatgtat tccggcagaa
     3121 gctcagtttc ggccggtcaa tagcggcatg gactctattg ccatttcaga tctctatttc
     3181 gttatcctca cagactccgg gaaagatgct gtcaggattg gtgtcggtac ctggaaggaa
     3241 caatacacta caactccatt cttttgtacc ttggaaaaca aggtaaataa gcgttccgtc
     3301 aggcctgttt tctttcgcct gggcaataaa gactatacgg acatgctgga acgaaaggac
     3361 atcaacaagg ccataaccgg tccattacac tagaggagat ttcttcaaat aacggtaaga
     3421 ttaaagagcc gtatagagcc cgacaatttt attgtatctg tattcccgat atttttaggg
     3481 aaaagtaaaa agggaatcaa gtacttttac ctgattccct ttggaaaatt tattaagcat
     3541 caccctttcc aaaaactata acattttgaa cgagacgttt ttctttttgt caaaatatct
     3601 ccttcattgt tgatgtaaag atgagaccat aattttttcc ggccgtcaca ggaagttgcc
     3661 aaaaatattt ttaatggttt ttctgtatag gattgatctt ggtcttggac ctgatgtgga
     3721 ggctcttcct ggaaggatca tcggttgaat aattcgagaa tggtggtttt tgtgactaaa
     3781 tgaacggaag gtgtttttgc aaattgattt tacaaaaaaa gccgaccact ttcgcgatcg
     3841 gctgctattt tattattttg agttgattgg ttccttagcc gttaagggca gctgcaccac
     3901 ctacgatttc agacaattcg ttggtgatag cttcctgacg cgccttgttg tagttgattc
     3961 tcaactcttt cagtaaggta tctgcgttat cggttgcttt atccattgct gtcattctgg
     4021 caccatgttc tgaggcattg gtgtccagca aagttttgtg aaactgtgtc tgcaagatgg
     4081 taggaaccaa atattccaaa aggctctttt ggtctggttc gaaaatataa tcggccttca
     4141 tatttcccct catagcgctc aatggcttct tgaggaccgg caggaactgt tcagcactaa
     4201 acagctgggt accggcattt ttaaacttac tataggagat ttcgatcgca tcgtattttc
     4261 ctgtagcgaa atcattcatc agtgtcgatg cgattggagc gatattgctg aatgaaagat
     4321 cagagaaagc attgatgtaa tcagtaatca ccggagcacc cttatgtact ctttttacat
     4381 aatcatatcc cttcttgccg atggtgataa aggtaacctt attggcgtcc agttgttcgg
     4441 catactttgt tcgggccaat gctgttgcct ctttgccgat gttggtattc aaggcgccgg
     4501 caagccctct gttggaggtg atgattacaa tggcgacatg tttgatttcc ctttcagcat
     4561 tgtatacaga tgaggcatct ccatccaggt tggaaagaat attggacaac atctcattga
     4621 gcttttcact ataaggacgc aactgcacaa tggcttgttg ggcacgacgc aacttggcag
     4681 ccgaaaccat tttcatggcc ttagtgattt gttgggtcga tataaccgac ttgatacgct
     4741 ctctgacttc ctttagatta gcacccatga ataagtgtta taatttataa tggtggagag
     4801 aagaaacgaa agtaaatttt atttcttccc tccgacttga ttttatttat ctggcaaaat
     4861 ttttggaaac agttgtggcg acggatctca atgtttcaag atcagcatcc tccagctttc
     4921 cggctttgaa atttgccagc atttcggggt gattcttatc taatgcagcg aagagattct
     4981 cttcgaattc acgcacatcg gaaggtttga tatccttcag caggttgttg gttcccagat
     5041 agatgattgc cacctgtttt tcaacactta ccggagaata ttgtgcctgc ttcaacacct
     5101 caacgttacg tacacccttg tcaagaacag atttggtggc ggcatcaaga tccgaaccaa
     5161 acttggcaaa tgcctccaac tccctgtact gcgcctggtc gagcttcaat gttccggaaa
     5221 cctttttcat agacttgatc tgagcgttac ctcctacacg gctcacggag attcccacgt
     5281 tgatcgccgg acgtacaccc gagttgaata ggttggactc caggaagatc tgaccgtcgg
     5341 taatggagat aacgttggtc gggatatatg ccgaaacgtc accggcctga gtttcgatga
     5401 tcggaagagc ggtaagagaa ccaccgcccc tgaccaggcc ggatttcttt agagattccg
     5461 gcaaatcatt catattttgt gcgatggcgt cgttgctgat tactttggca gcacgctcca
     5521 aaaggcggct gtgcagatag aatacatcac ccggataagc ctcacgtccc ggtggccttc
     5581 tcaacaaaag agacacctca cggtatgcaa ccgcctgctt cgacaaatca tcatagatga
     5641 tcagtgcagg acgtccggta tcccggaaga actcaccgat ggcagcacca gcgaaaggag
     5701 cgtagaactg tagcggagcg ggatcggcag ctgaagcaga aacaatggta gtatatgcca
     5761 tggcgccatt ttcttccagg actcttgcga cctgtgctac ggtagaggat ttctgaccac
     5821 aggcaacata gatacaataa acaggctctc cttgctggta gaattctttt tgattgatga
     5881 ttgtgtcaat tgcaatagcc gtctttccaa tctgacggtc accgatgatc aactcacgtt
     5941 ggccacgacc aatcggaatc attgcatcaa tcgcctttat acctgtctgg agcggctcag
     6001 tcaccggctg acggaaaata actccgggag ctttacgctc caaaggcatt tcatatttat
     6061 cacctgtgat ttcacccttg ccatcaattg gctggccaag cggattgacc acacgaccca
     6121 gataaccttc tcccacctgg atggaagcaa tggtattggt tctcttgact tttgaacctt
     6181 ctttcaggtc gtcagatgga cccagcaata caacaccgac gttgtcttct tcaagattca
     6241 atacgatagc ctgaaccccg gtctcaaatt caacgagctc tccggccttg gcattagtaa
     6301 gtccatagac acgggcgata ccgtcaccta ctgacagtac tacaccaacc tcttccagtt
     6361 ctgcttcggt atgaaaaccg gaaagttgct gcttcaggat tgctgagatt tcatcaggtt
     6421 taacattaac catggttatt ataagtttta ttttttaaat aattaattgt gggtgagtgt
     6481 ttgcttcaat ttattcagtt ggtaagccac actactgtca tatagtctat ccgcatactg
     6541 gatgataaat ccgcctatca ggtccgggtt taaggctgtc tcgagttgga tttgcttatc
     6601 actaccgacc agtcctttta ttttgtccag gatgatgttg gtagtggctt catcgagcgg
     6661 ggccgccgag gtaaccttta ctaaggcgat attaaacaga gtattatact gttcggtaaa
     6721 ttggtctgca atatctacca gcaatgattc ccggcccttc ttgatggtca gatcgaagaa
     6781 tgacatcgtc aaggtatcta gttttccctc gaagacagct ctcaacgctg caatcttctt
     6841 gtcggggaaa atgatagggc ttttgagcag gaggacaagg tctctgcttt tcaaggcttc
     6901 tttgaatact gccatgtcac cgcgcacagc ttcggtcttt ttctggtcta tcgccagatc
     6961 aagcaacgac tttgcgtaac gcgatgcaac ttgtgataca gacatttata atgattttcc
     7021 ttggatgtat taattcattt taatctcatt cgctaatgac ttcgccagtt caatctgaga
     7081 tgggtcattg ctcaattgct tcttgatcac actttctgct acctgtacag ccagcaagcc
     7141 aacctggcct ttcagttcag agatggcggc ttttttctga ttctcgattt cgagattggc
     7201 attggctaag atacgaccgg cctcttcctt ggcttttgat ttggcatcag agacgatctg
     7261 tgcagcattt tctttagcct ctttaagcat ggccgctttt tcttcacgag cctgggctat
     7321 taactgctca ttttcagcct tcagattgtt catttcgttc ctggcgatat ctgcctgcgc
     7381 cagcgcgtcc ttgatggagt cttcacgctc cttcaaagcg ttggcaatag gagtaaatgc
     7441 ctttttaccc aggaagaacc aaagggataa gaaaatcaaa acagtccaga aaaacaatcc
     7501 aaactcgggc ttgatcacat tgaattcggc taataaaacc atattatttt gattgaaatt
     7561 tttttaaaat aagcccgtca ttaaaccaat cgtcttcacg acgggcagat gttttaggat
     7621 ttaattatcc tgctgtaagg aatgcaacaa ccactgcgaa aagcgcaacc gcctcaacga
     7681 aggctgcaat gatcaacatg ttgttacgga tatcaccaac agcttcaggc tggcgggcaa
     7741 tcgcctccat acctttacca ccgataaatc caattccgat accagcaccg atagccgcca
     7801 atccggctcc aattcctgcg tacataatgt aattatttaa attgtttgtt attcaattaa
     7861 aatttcaaat tcgggcaaga aaactttttt cctcgctttt tataatcagt gatgtgcctc
     7921 ttctgtcgct gctccgatgt aggatgcaga aagtatcgcg aaaataaacg cttgaataaa
     7981 ggcaacaata aattctatta cgtttagaaa gagcgtaaat ggtattgcga gtgccgttcc
     8041 ggcaatggct ccacccatac ttgctccgac ttttccaaac acaaaaatca atcctattaa
     8101 actcagaatt atgatgtgtc ctgccgtgat gttggcaaaa agtcgtatca ttaaagaaac
     8161 cggcttgatg aatacaccaa gaaactcaat cggtgccagg aaaagcttca tcgccggagg
     8221 cacgcccggc atccagaaga tatgcttcca gtagttaccg ttacccagga tgttggtgac
     8281 cagaaaggtc atcacggcta aagccatggt cacggctatg tttccggtga tattggcact
     8341 gaatgggaag aatggaatca gtcccatcag gttacataca aggatgaaga agaaaatagt
     8401 caggataaag ggaaggtgtt tctggtatct gtctccgagc attggcttgg cgacctcatc
     8461 gatgataaag agaacgataa tctcgatcag gttctgcaaa cccctggggg cttgtccgga
     8521 tctgtttcta tatgccttgg agatggataa aaacaggatg acaagcaaga ttgccgccaa
     8581 aagcattgaa aagacaactt ttgatataga gaaatcatag aatgaagtca tgccggtaaa
     8641 ggcagaggct ttttctagcg tgtattcctt tccctgatgt tccagtgttg ctacctctac
     8701 ggatccctcc ttggtatgtt tgatgtggcc gatctctaca tgacccatgt caggaaagtc
     8761 ggctggaagc ctttttacaa ttccttcatc cagcacataa cggttatagg ccttatgtcc
     8821 gtgctccaac ttagacgaca taaacattgt ccagccatct tctttggaat aaaggataca
     8881 tggcagcggc actgctatgt gaccaaagat gtggaagtcg ttggaattgt tgatgtggtt
     8941 gaagatcagg tcgttcatct tgaattcttc ctcttctttg aggacacctt ccactacatc
     9001 atgtacttcc cctgccggag catcatggct atgatcttgg gcaaaattca ccaccggtgt
     9061 aaccaggatt gtgaaaagca ctaagaaata cagccgggga aagactgaaa agcgaatatt
     9121 gagagatgaa tacaactttg tcatatgcgt taaattgctt agcctgcaag ggttccatgc
     9181 aggtttttca aaattcggca caaaggtaag ctgtgagtca aatataacca aggattttac
     9241 cccctctttt ttagaaaatc aatagtttat aaacataagt tgattcacgg tttgttgtcc
     9301 tgctctataa aaaaatatcc ggttgttttt attccgggga caccattttc ccgtctaagg
     9361 aaactccaca gaccatcgaa aaatcaggag ctatctacac caaattgatt ctgatcgttc
     9421 tgtcagccat ccggattata gccatcccga tgtactttta cttttaaaca aagtgtaatc
     9481 gcttttccca cgtcaacccc gcttccttaa aaactattgc ctgcgtcgga cttgcaacag
     9541 aatacagtaa tttttcctgg aatgatacat ctaccccaaa ggctttaaga caaaagcagc
     9601 cgggaatttg attttcaaaa gatacaggaa gcgattggat ttgaaatatc gacgtatcaa
     9661 aaaaatctgt agtacacaaa ataaacattt atcagcggaa tgcccctgtt tacgcaggat
     9721 cagatctgca ttaacgcaat gtgggtcatt caggtggctt taaaatggaa tgttctatag
     9781 cccatactat tttacgtaag tttaaaagca gagtaggata tcattgattt ttttcaggaa
     9841 ttcaggtgac caaagatatt tctgcaatct catcatattt tcctgttatc ggtggtaata
     9901 agagatgctt tcttgaattc aggtcggatc aacttcatgg aatcatatat cacaccaggc
     9961 tataacccta tttttttcta aaacttgcta aaagtttctc ccaatattgc ctttcttcaa
    10021 catttaattc tttagaatgt aatgcaagta aataactaat ttcctcgatg atgcgatacc
    10081 tctttatttg atcaatatct gataatgctt gagcaacagg agtgaaggga tcatcaagtg
    10141 tgagtatcat gtagagacct tcagccgggt ccgctaatga agttgccgta ttgattgttg
    10201 taccaataac caatgttttt tccagaagac taccaatttc ttctactttt ttaattgatt
    10261 tcattgtttc catagaagcc ttgccaaata aaatctgttt gccggaggat tgaggcttaa
    10321 ctttttttgg cgccggtttc ttttttttgc ttctctctgc ttctattttt ggggaaaggg
    10381 ttgaacctaa ttcaataatt aatttagaaa gctcttcagc atagatcaaa tcatcctcat
    10441 cgagggcccc ctttttattc ttaaaatcaa agagataagc atctaagtcc tcaacaccga
    10501 gagacaactc ttccaatagt gatttgtctg cacccagacc cgccatttct tctaaccaag
    10561 aacgcagtac tgactgtgca taactaaatt taccggccac aagactatat gtaaattttt
    10621 cacctttttc ccggtcaaca aaaacgggta atgagtctaa atttgggtac ttctctgagt
    10681 aaatatcggg tttatcatct ggcgcttctg ctttggtgtc acccgatccg gcttttttca
    10741 tttggacaat tttctgcgtg cctgaagaag ttaaatgaaa ttccggtggc acctgcatat
    10801 ttttgataga tgccgagaag ggccgcccac tcgaaacttc tgacaaatca gaatccgttc
    10861 gaatcggacg atgacttatc ggtggataca gatttcggcg cataataatt gaaaattttt
    10921 attcagaaaa tctacaaagc cggataataa aaacaaaaga gattctctca gtgtggcatt
    10981 taacgagcct tcaaaaatac aaaataatgc tggtattaat ttatttgttt aattcctatt
    11041 taatttagga aagtgctgcc caacagtcgc ttgaaccttc tttctcctta ccgatgtctc
    11101 ccaacttaga ggtaaggcac ttgcacaaaa gctcaaagcg aatcacgatt gccctataag
    11161 catgtatagt cccccaaaag tcaggtgctt ttacgaagac acaattttta ccttaggatg
    11221 cgaagccgga aaattaaaga aaggagtacg gcaggctcgt tgatgtctat atttttcata
    11281 atgacacaat cgaaatccga tttgtaagag gtttattatt tcgaaattag ttataaaata
    11341 caacttcgat ttccggtttg tcattcgcct caaacgccta taaacagggg ctttctacca
    11401 ttctttaccc gtttgtaagc gcttacagtc gtttacaaac gactacaaac gtttatttac
    11461 cggataaata ttgacatgtg tttcatcttt gccttgtcaa tcggaatgaa tgacttttgg
    11521 gaataaatcc gggcatcaat tcattgcgaa taaactttta attcatatca taaacttttt
    11581 aaatcattac attatgacaa cgctaagcaa ccaagtccaa ctgatcggaa acgtaggtca
    11641 gcaaccggaa atcaaaactt ttgacaatgg tggaaaaatc gcacgggtaa gtctggcaac
    11701 caatgagaag cgcaagaatg cgacgggtga gtatatcacg gagaccacct ggcacaatct
    11761 cgtattccgg ggaaaacacg cagaaattgt ggaaaagcac gtcgaaaaag gtaaggaaat
    11821 cctggtagaa ggaaagatca ttaatcgttc gtatgaagac aaaaatggtg ataagcggta
    11881 tataaccgaa atccaggtcc ttaacttcaa gtttttggga gggcgtaatt agatcggggc
    11941 cggtgtcggc ttggttaatc tgactgagtc acacctttcc cgtcttggca tcaatgcctg
    12001 aaatcatcta caacctgact gtttccggtg ttgatgcctt tcgatatcaa tgacaatgct
    12061 ttggattgga tggccgggcg gatgaggcac tattgtttgg taaaacacag aaccgcttgt
    12121 ctgctgatac cattgccttt tgataagaat gtcatccggg ccaattgtaa tttggttatc
    12181 caactttctc tgttatggct tctttgctct ggtgtgccgg tgcctggtca tcggcctcca
    12241 aacagaaagg gcacactacg aattgaattt gtttcaaaat cccggtaaat atgggaaacg
    12301 acctgcccca agtgatgaaa gcattaaccc ggctatactt ttgattcaga caacgaggtc
    12361 tatggaagca tggaggataa agacaaccgg tgtacggtat ggtttggcct tgttttaccc
    12421 gggagggatt ggttgtcatt gacggtcaag agcacaacac agcttcaaat cagcaatctt
    12481 caggtagtgt ggtaatgttc gaaaaaggta atgttcaaaa aaggtaaggg aatgttcaaa
    12541 aaagcaactg tataccagga tgattcatat aatagtccat aaaatctagg aatactagtc
    12601 ccgggatgaa acaatccggg gttcagatat acttttccgg attgctatgc tactagagcg
    12661 ctgctctgtc ccgggagtga aacaatccgg ggttccggga taccgaggtt acagtcgtat
    12721 agaccaattt cattgattta taacgaatgt gccttcggtt ttatcttcaa tttgcgttga
    12781 aacgtgttga gtttgttaaa tttgggttaa aatcgtgttg aaatcgtgtt gaaatcacac
    12841 aaaataaaaa agggcttcat tgctgaaacc cttatcatca ttgagtagcg aggggggggc
    12901 ttgaaccccc gaccttatga ttatgaatca tacgctctaa ccagctgagc tacctcgcca
    12961 tggtttgggg cggtgaagat aaattcattt tttcaaacca aaagcttttt ctggtttttt
    13021 attctgtaat actctgtacc aggagagaaa tattgttgtt aagaacttca ataaagcctc
    13081 cttgtatttt aaatgacatc agcttgcctt ctgcggtctt tacttctacc ttgccttcgg
    13141 caagtgctga cacaattggc gcgtgattat tcagtatctg aaattcaccg gacacaccgg
    13201 gcaccttgac tgaagtaatc tcaccgttga aatattcaac gtcaggggtc aatactgata
    13261 gttgcatctt ccgattttta ttatttcttg ttcctctctg ttatgcatta gcttctgcaa
    13321 gcaacttttt acccttggca atggcatcat cgatggttcc taccaggttg aaggcattct
    13381 caggatattc atccacctct ccgtccatga tcatattgaa tccgcggata gtctcctcaa
    13441 ttggaaccaa aacgccctta agtccggtaa actgttcggc cacatggaat ggctgagaca
    13501 agaatcgctg taccctacga gcacggtgta ccaccagttt gtcatcttct gaaagctctt
    13561 ccataccaag gatggcgatg atatcctgca attcattata acgttgtaga atacccttta
    13621 ccctttgtgc acaattataa tgctcatgac cgataacatt cgggtcaagg atgcgggatg
    13681 tcgagttcag tggatctaca gccgggtaga taccaagtga agcaatcttc ctgtccaata
    13741 ccgttgtcgc atcaaggtga gcaaaggttg ttgccggagc cgggtcggtc aagtcgtcag
    13801 caggcacata gaccgcctgt acggatgtaa tggacccccg cttggtagaa gtaattcttt
    13861 cctgcataag acccatttca gtagccagtg ttggctgata acctaccgct gaaggcatac
    13921 gtccaagtag tgcggatacc tctgaacccg cctgcgtaaa tcggaatata ttgtcgatga
    13981 agaagaggat gtcccttcct ccgtttggat cattaaggtc tccgtcgcgg aaatactctg
    14041 caaaggtcaa tcctgacaat gcaacacgcg cacgtgctcc cggcggctcg ttcatctgac
    14101 caaacacgaa tgtcgcctga ctgtttttaa gcaactcttt atccaccttg ctgaggtccc
    14161 atcctccttc ttccatcgaa tgcttaaatt cgtcgccata ttttacgatg cctgactcta
    14221 tcatttcacg caacaggtca tttccttccc ttgtacgctc tccaaccccc gcaaatacag
    14281 acataccgtc atatcctttc gcaatattgt taatcagctc ctgaatcaat accgtctttc
    14341 ctacaccggc acctccgaac aaaccgatct taccaccctt ctggtatggt tcgacaaggt
    14401 cgataacctt gataccggta taaagaatct ccgtctctgt actcaattgc tcaaaagtcg
    14461 gtggtttctg atggatggag taagcattgc tgcctttctc tacttcaccg ataccatcga
    14521 tcgcgtcgcc cacgacatta aataagcggc ctttgatatg gtcgcccacc ggcatggaga
    14581 tggtgccacc ggtatcgatg acttcagtac cccgctgcaa gccgtcggta gcgtccatcg
    14641 ctacagtacg cacactgtct tctcccagat gttgctgaac ttcaaggata aggtcattgg
    14701 tgccttctct cttgataacc aatgcattaa agatatccgg aagcttagag ccttctccgg
    14761 aaaagctaac gtcaactaca ggaccaatga tttgtttaac gcgacctatg tttgccatgt
    14821 ttttttcttg ataaatttag taaaatcaat ggtttaagtg gccttgcccc aaaaacggtg
    14881 caaaaatata acaaccaacg cacctaacca aaaagttttc actattttta taaattactg
    14941 taagatagaa caatgggtct tgacaaattt ctgtcattac agcttcctgc tatgcttcaa
    15001 cgttttgttc cgcccggata caaatttttc aatgcttccc gtcgactcaa tcccgacatt
    15061 tcatgatgct ccacaaatga ccgaacacgt gcaggttccg tcttggcata ttcccgcaag
    15121 gcccagccaa tgccttttcg gataaagaaa tccttgtgca agaggtgagg cagaataaat
    15181 tgctgaagca tttcccagtc agtggaagac ttgtatttca gttgcgtgag gattgaagtc
    15241 ctgatcagcc agatatttcc ggaggaattc cacgcataca gatcgtccgt agtgagcatc
    15301 ccgtcttgat aggatctgcc tgcagtatgt gtggcaagaa aatctacggt gtcccaccag
    15361 gacttttcgg tgagtgcttg ttctaatggt tttttgatgt cttttccgaa tttttttcgg
    15421 tacgtatcca acagttccat ccccgtatat tgaaactccc tttcttcgtt ttgccaaagt
    15481 aattcaagaa ggatggttaa tgcttgtgtg tcaaaaatac gggcattctt aataaagggt
    15541 ttcacgataa gacgccgttc tccctgcttg ataccaaatg acccgaattg tcccttcagg
    15601 tatctgatga tatgcacggc attggcttgg ttgcgatggc cggccagctg tgattgcagt
    15661 tcggaaatcc agatttcagg cgtcatggct tagtactttt tcgatttcat tgtattcaaa
    15721 tccacgggac ataagatatt gtgtcagctg gtagggagac tgggttttct tttccttcat
    15781 tctacgctcc aggagtcgtc tgatgagctg ctgatattct tcttcatcta taacttccaa
    15841 tcccttgcgg atactgtaat cggaaacctg cctttgcttc aaggcatagg tgatcttctg
    15901 acgcccccaa tgattgatgt taaacttgcc cgaaacaaag gcgcgggcat accgttcttc
    15961 attcaggaag ttctcactaa tcagggtggc aatgatttgt tccagttctt ctccaaaaat
    16021 cttccactca atcagcttgc tgcgcacctc ggagtggcat ctctcctgat aagcacaata
    16081 ttttctggcc ctttccaaat tgatcgctaa cattatgcaa aggtaatcgg gaaaaggtaa
    16141 gaaaggattg attgatgtgt atatttaatt ggattaaaca cgacaaccat cctcccgaca
    16201 caactatctt ccaattatta ggtattaatg acggaaatgt tatcttcgca tactttacag
    16261 caaaaatttt tataccatgc aggtcaaagt aatcaacaag tcgcgatatt ctcttcctca
    16321 gtacgagact gccggtagcg ccggcatgga tttacgggcc aatacggata tgactcttga
    16381 actaaaaccc ctggaacgcg cccttatccc caccggatta tacatcgaac ttcaacccgg
    16441 tacagaagct cagataaggc cccgaagcgg actttccatc aaaaaaggac tgaccgtaat
    16501 caatgcgccg gggacaatcg acagtgatta tcgcggcgaa atcaaaattc ctatgatcaa
    16561 tctgtccgat gaacttcaga ccatcgatcc gggagagcgg gttgcacaaa tggttgtggc
    16621 aaaatacgaa accatctcgt ggatggaagt cgaagatatc agccctacgg aacgcggctc
    16681 cggcggattc ggttccaccg gggggtaatc catggacaaa agctaaagga aggagagcat
    16741 ccggatgatt gaaaagtccg gcgcccaaga cagagatcaa atccctgcga taatatgtgg
    16801 cggcgcaatc ttgtcaataa aatccctcca acaggagttc tttatttctc aacatcatat
    16861 tcgctgcgtc ttaaaacttt ttgattagct atatcgtatt ctatattata acgtacttgt
    16921 ttactggcca tttttctatc cataccccaa tgaacaaccg cagaaaagta tgctttatcg
    16981 tctgtacaag ctactaaatt gagctttgtt tttcccaaat ctgtttcttt aatttttgta
    17041 gtataaagca ctttacctgt ttcagcatca ataatatcgt cattgaccaa accgtccttc
    17101 aagtatctgt ttgcgaaaag cttttgacct attagattaa gtccattagg gtcggcgggc
    17161 tgcggtaagg gataattgta agtccacaat acactgccat cttcagtact caacctgctc
    17221 actttgttcg tatggtttac ctgaagcaaa tatttgaggt cataaataat atagccgcca
    17281 ttggtgattt cttctgtttt gataatcttt cctgtttttg gttcaatgat tgaatagcag
    17341 cccagttgcg gatcgccaaa gctcgatttt aattgtatca attttccatc aataagtgta
    17401 ggtactttca gctgtatggt agcatcattg accttaaacg gcgtaaaagc agggtcgtca
    17461 taatgcaaat gccatttcat ctttcgggtt ttaatatcga aggctgttaa tccgagataa
    17521 ggttttcgga gttcggcttc ttctggagca gatttcaaca gacccaccga cggtttcatg
    17581 ctaacataaa ggttttcatc atccattgtc ataaaaaata ccgcatcatc gcgataattg
    17641 aacggaagta tttttgtaga gtcaatatac tttttgtagt atgtataatc tacctccttt
    17701 tcgtgataga taggcacttc aaatgtatca atctttgcat catttgcaaa tcgcttttct
    17761 tcagtaggag gcgacataca agccttaaaa atccatgata gcaatcccat aaaaacaaaa
    17821 atttgatatc gcacaatcat ataattagtt gaaaatttag catataaagt tataaaaaag
    17881 aaaagaattt ggtattgtta aattagtata ggcaattcat tattcagttt caaaaacaga
    17941 agattcaaag atttttcaat catgtgttga gccttgctgt aacattttcc ttttctctta
    18001 aatcgtgcaa ggtagtgtcg aattctgcta ttgtagcctt ctactgagta ggtttctgct
    18061 ttacttctga tatgaaattc tgatgctaag atttcttcgt atgccttcca atggtccata
    18121 cagtaaaacc ctacttttat ctctttaaga ttattgtaga gctttgttcc tgtttctgtg
    18181 cctctgctcc cacacacaaa cgagatgaac cgtcttgcaa atcgatcaac agcaatccat
    18241 acccaacaat agtttttttt gaaccgacat agctatgcag ttcgtctatt tctacgactg
    18301 caatgggttg gctgttttga ggaaggctca ctttgctacc ccattccttt atccaccgat
    18361 atactgttcc gtagcttatt ttcaatatgc gtccaatagc ccgaaaaccc aatccttcta
    18421 agtacatttc taatgccatt cgtctaactt ctgccgtctt gacatcagat ttcttttcaa
    18481 cagtatagtg cattccacat tctttacata aataacgctg ccttcctttt acaattccgt
    18541 ctttgcagtg ttgtgcgctt ttgcatttgg gacaattcat agtgataatt ttttaccaca
    18601 aaatcaataa ttctatatta aattaacaat acccttttta taattgtcct ccaacttcct
    18661 taaacttctc tacaaaggat accagttttt gaaatacgct ttgcttcttt gtcaaatact
    18721 gaggatttaa aggactcatc ttcggcagaa gtgcattcaa ttctgttccg ttttcacttg
    18781 catattttcg ttttaatgat gtcgttatat atcgcttcgc ttcctcaata ttcagatttt
    18841 cttctgtgat taattccaat gcttccgctt tctgcctggc ttgagcaaat tgcaaaaatg
    18901 aatcaataac attcgctttg tcctgaaggg tgtcaaggtc agtttcatgg atgaaatcaa
    18961 ctattaaact ttctttagcc ctattcccga cacttgcgcg gataatcctg cgtatttcag
    19021 ctattaattc gtctttgtcc ttcgttttct tgttatgttg aaaaatcaat tcaaggatgt
    19081 agtcaagatt tatctcctgt gattttagta agtcaacctc aaataccaca tcatcccaat
    19141 caatggtaga tttttctgcc tcccttccgc ttctttcacg tcttaaccag tctcgtatgt
    19201 cattgtaggt ggaacgataa tcctgaacgt tcctatcttt aagcaattca atgttgcgca
    19261 ttgcagcaat gtcctcatcg gttataaaat gagccttttt aaattcttca attgcttccg
    19321 ggtcattttt gtctattgcc tgaaatgctt ttaaatgggt aaattcatcg tagttttgga
    19381 ggatattttc tactcttaaa tattctccaa acagcttaac aaattccttt ttgtcgcctt
    19441 cctttacaat ctcgtccggg ttgggaaatt ttgtcttcag ctcatttact atttcattat
    19501 aacctctgcg tgcgtcaccg gtaacaatat ccgtaaagcc ttccaaatat tctttgtagc
    19561 tcttttcgag aaccacgttt ttagtgttgc tgtcgccaaa aagtgtaatg gcgtcaatgg
    19621 tcgctttttc caagtcacgg aaggtaacaa tattgccaaa tgccttattg gggtaaatac
    19681 gattggttct tgaaaaggcc tgaattaagc cgtgataacg caaattcttg tcaacaaaca
    19741 atgtgttgag tgagggtgca tcaaaaccgg tcaggaacat tccgacgaca atgattaaat
    19801 caatctcctt gttctttaca cgtttggcaa ggtcacggta ataattctga aatttacctt
    19861 ctacattgaa gttcatttta aacattgcat tgtagtcgct tatagctttg gttaaaaact
    19921 ctttggcact aacatccatt gccgtaggct caaaagtctc atccactata tcgccgatgg
    19981 cactttgttc ttcgtttggt gcaaaagaga agatggttgc aattttcaaa ggcttgtaat
    20041 tagttgctga gcttgtcgaa gcatcttttt gcagattgtt taattcctca taataacatt
    20101 tggctgcatc cacact
//
LOCUS       DAOQQN010000009        17519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1005_length_17519_cov_5.299817, whole genome shotgun sequence.
ACCESSION   DAOQQN010000009 DAOQQN010000000
VERSION     DAOQQN010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 17519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17519
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1005_length_17519_cov_5.299817"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PLG24_00755"
     CDS             <1..354
                     /locus_tag="PLG24_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HMZ23437.1"
                     /translation="RIVPDGKEVTVTRASWDMVRYKLDENDNGKIKPEVVGSFEQFPL
                     KLAWAMSIHKSQGQTFDRVILDMEGGAFEFGQTYVALSRCRTLEGIVLKRPLKPRDIM
                     VDQRVVDFYFDHFRG"
     gene            508..1032
                     /locus_tag="PLG24_00760"
     CDS             508..1032
                     /locus_tag="PLG24_00760"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMZ23438.1"
                     /translation="MKLPELGTHNPFYQFYFNLVPQGDLMEIFMQQMDEFSEFIRSIP
                     VDKLDFAYAPGKWTIREAINHVSDTERVYAYRSLVAARMDSQTNLTSFDENAYALHAD
                     VSNRSVDELIADFRAVRQSSVYLFKFLNDTQASFQAKNGNHVFTANGGLCFMIGHVIH
                     HENVIKSRYLNIGD"
     gene            1548..4070
                     /locus_tag="PLG24_00765"
     CDS             1548..4070
                     /locus_tag="PLG24_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HMZ23439.1"
                     /translation="MLSQQVAIDNVKPVARAVQHAKSIKAFPKVDLFVEDLSLDVRDK
                     YPDMLSQFSYLKLRKEALNSFLNTRPDAINIQVPYLSQNIQLELVRVDVLSDDFKTYT
                     SDSPEKAVNYSPGLYYRGIVEGDVSSTVAISFFEDDVIGMISTEELGNITFNKMEDAD
                     RYLIYSDRDLLVKNPNFCNTEEPENYAEQVRQYMNNSIEVRDGKCVKVYIECDYALYQ
                     NKGSVTNVNNWISAVYNNVATLYANEQISTQISSTFVWTSSDPYSKTNSYTALTQFRN
                     NRPSFNGDLAHLAALGGQNIGGVAWVDALCTSYKYAYSNIQATYQTVPTYSWTVEVMT
                     HEMGHNLGSPHTQSCSWPGGALDNCYTTEGGCPPGPPPTNGGTIMSYCHLTSYGINFN
                     NGFGTLPGNLVRSRVNAASCLGTCSGGGTCNAPSGLVVTNITNTSAVVSWNTVSGATA
                     YQLQYRATGGSWTVLSQQTTTSVTLTGLAGSTTYETQVRSICSGGNSAYSAIVTFTTS
                     GGSGCSVPTGLAATNITNTSATISWNSVSGAVNYTLRYKLTSSGTWNTYTTSSTIVNM
                     TNMTPGTSYDVGVRTNCSNGSSNYSATVTFTTTGGGASYCTSKGNTSSYEWIDLVKLG
                     SINNVTGSNGGYGDFTALNTTLSAGSSYTMTLSAGMNGSYTEYWTVWIDYNRDGDFLD
                     PGEKVTSFSSSSKGNINRSFSVPTTASNGPTRMRVQMKYGSYGTSCETFSYGEVEDYT
                     VTISGGSNLPSAITNTITDYMVYPNPSSDVFTAQFEAGSSMEATISIMDANGRVVSDQ
                     LVNTIAGKNIVPLETSGYLVPGMYLIRIVTKFGHQAIRVVKM"
     gene            complement(4230..5375)
                     /locus_tag="PLG24_00770"
     CDS             complement(4230..5375)
                     /locus_tag="PLG24_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMZ23440.1"
                     /translation="MFRKMRVAMIGGGRNAFIGSIHRIAIQMDGLAEIVAGALSANPE
                     NALASGKDLFLPEDRIYTDFRTMIKKESASPSEIRPDIICIVTPNHMHFEPAMLALQN
                     GFHVAIDKPITFSTEEAIRLKEVLESSGKQLLLTHVYTGYPMIKQAREMIQSGVLGRI
                     RKVFVEYPQGWLSAPLEQENNKQASWRTDPTKSGKAGAMGDIGTHAFNLAEYVSGLSV
                     YKICADVNTIVPGRLLDDDGAVLLRFEGGASGVLMATQVATGEENNLRIRVYGEKGGL
                     EWQQADCNSLRVMWPDKPAEIFRTGAGYSGAMAVHNSRTPAGHPEGYLEAFANLYRNF
                     FHTIAASDKQESEKYLDFPGIEEGIRGMKFVDKVIESGVSEQKWLTF"
     gene            5538..6767
                     /locus_tag="PLG24_00775"
     CDS             5538..6767
                     /locus_tag="PLG24_00775"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HMZ23441.1"
                     /translation="MKPTVRIQLNSMMFLNFFVWGIWFVTLGTYLLKGDIKASDAQVG
                     LAFGTQCLGATIAPFIIGLIADKYFAAQKILGILHLAGAVLMYLISGSKEFTGFYTLL
                     LIYMVIYMPTLALCNSIALNQVSDAEKEFGGIRLWGTIGWIVAGQVVGFLALEKNPGD
                     LVMTFKIAGAISLVFGIYSFFLPDTPPPKAGKDVSFREIVGLDALKVLKIKNYAVFFI
                     ASILICIPLAFYYGQANIFLNEIGMQGAAQKMSLGQMSEAVFLFLMPWFFRRLGVKYM
                     LLVGMLAWVVRYLCFAYGDLDSGLWMLYLGIILHGICYDFFFVTGNIYTDQVAGPQVR
                     SSAQGLITLATYGLGMYIGFWAAGAIAGANVILDAGGEVTNHHWDKVWLFPAMFAAGV
                     TVLFLLFFKKSEKQDIA"
     gene            6764..7660
                     /locus_tag="PLG24_00780"
     CDS             6764..7660
                     /locus_tag="PLG24_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HMZ23442.1"
                     /translation="MKKNIHGRREVLKKLSLGGLALTGVGKLSAFNNDSEESLAWKGN
                     IRHSVCQWCVSFLTIEEMALWIKSIGVGAIDLISPKDWNILKKYNLECSMCYIDGKVS
                     LTEGWAQRENHARLVEDYIKSIPMVKDAGYTNLICFSGNRKGMDDETGLKNCEEGLKK
                     IMSLAEKNNVIIQMELLNSKVDHHDYMADKTAWGVELCKRIGSPNFKLLYDIYHMQVD
                     EGDVIHTIRDNHQYIGHYHTAGVPGRNEIDDTQELYYPAIMKAIVETGFKGYVAQEFI
                     SKGKEKDEKLQAFKRGIEICDV"
     gene            complement(7896..9095)
                     /locus_tag="PLG24_00785"
     CDS             complement(7896..9095)
                     /locus_tag="PLG24_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMZ23443.1"
                     /translation="MPKLSEKATLMPPSPIRKLVPYAETAKRQGKHVIHLNIGQPDIE
                     TPEIAREAVKHADIKVLEYSHSAGIESYRKGLAAYYSANGISVTSDEIIVTTGGSEAL
                     LFAFMTCLNPGDEIIIPEPFYANYNGFAVSAGIHVVPVTASIDNGFALPPISEFEKKI
                     TPKTKAILICNPSNPTGYLYSREELEHLREIIQSHDLFLFADEVYREFCYDGATPFSV
                     MNLKGIENNVVLIDSVSKRYSMCGARIGALVSKNKEIMQAALKFGQARLSPPTYEQIA
                     AEAALDTPKSYFDDVIKEYVGRRDLLVDGLNKIKGVRCPKPNGAFYCIAEFPVEDTEH
                     FCQWLLESFEFEGNTVMMAPLSGFYATKGLGKKEARLAYVLKKEEIEAAVRCLEKALE
                     VYPYTSY"
     gene            complement(9098..9640)
                     /locus_tag="PLG24_00790"
     CDS             complement(9098..9640)
                     /locus_tag="PLG24_00790"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HMZ23444.1"
                     /translation="MEKDSEQLLSKEEIHLDKLRRIVQKSIEEENLIMENLLHPPSEI
                     LTIGQKISDKVARFGGSWAFIITFFIILTFWIILNYTLPKSDQFDPYPFILMNLLLSC
                     IAAFQAPIIMMSQNRQEEKDRKQNINDYMVNMKAEMEIKALHQKIDLLLEEQIKVLFE
                     SHAKQLAILESLQEKIEKQS"
     gene            complement(9956..10255)
                     /locus_tag="PLG24_00795"
     CDS             complement(9956..10255)
                     /locus_tag="PLG24_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HMZ23445.1"
                     /translation="MADKYILIRTICTHYGLEQDFLDRLNETSLISYHIKDQDIYINK
                     DQIGNLEKIIRLHYDLGINFEGIDVIFNLMERITEMEAELKLLKSHFSSFNDNLL"
     gene            complement(10255..11181)
                     /locus_tag="PLG24_00800"
     CDS             complement(10255..11181)
                     /locus_tag="PLG24_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HMZ23446.1"
                     /translation="MEYIDYYKILGLDKTATSQEIKSAYRKLARKYHPDLNAGDKEAE
                     KKFKEINEANEVLSNEENRRKYDKYGKDWKHAEEIERMRRQQGSRASSGNQGYGKGYE
                     GFSGFSDSGFEDDDFSDFFHSMFGRTSGGRSRTSTRFKGQDYNAVLHLNLTDVYHSKQ
                     QVIEVNGKKLRMTIPAGVEDGQTIKIAGKGGPGANGGPDGDLYIRFEIHNDTDFRREG
                     ANLYNQVEIDLYTALLGGEVTVKTFNGQVKLKIAAEMQSGTKVRLKGKGFPVYKEKEK
                     FGDLIITYLIKNPTNLSEQEKDLFRQLKKLRS"
     gene            complement(11288..12052)
                     /gene="map"
                     /locus_tag="PLG24_00805"
     CDS             complement(11288..12052)
                     /gene="map"
                     /locus_tag="PLG24_00805"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HMZ23447.1"
                     /translation="MLIKTKEELDGIRKAGQTAGEILKKMRQYCKPGLSTKEIDLYGG
                     RLMKEYGVRSAPILAYNFPGFTCISVNDVVAHGVPSTQTILREDDLINIDVSIEQDGF
                     WADNGGSFTLGQQNSPYDHLIDASRDILKQAIFRIKHGMRVSELGGYIHDSAKKVGYT
                     VIKNLTGHGVGRSLHEEPRSIANYREAFNFRRFRENEVVAIETFISTKSEFTKTDNDG
                     WTLRGDKGGFVAQHEHTILVTRQEPEILTLSNEIWS"
     gene            12135..12539
                     /locus_tag="PLG24_00810"
     CDS             12135..12539
                     /locus_tag="PLG24_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HMZ23448.1"
                     /translation="MLNNIVYSLRDKDMVTMIMYGIPNCNSVKKARVWLEHHNVEYTF
                     HDYKKLGISSEKIEAWLGQIPLERLLNKAGMTYRNLNSIEKESASEKNGAIAIMIEKP
                     SVIKRPLIEDESGKVMCLGFSDELYEDLFNGK"
     gene            complement(12608..12961)
                     /locus_tag="PLG24_00815"
     CDS             complement(12608..12961)
                     /locus_tag="PLG24_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HMZ23449.1"
                     /translation="MSKNKKNRDGVVYSTDPDYNYNYFSDLQPVEQGFDPAMMKLRVA
                     IDRKQRGGKEVTLITGFRGNESELNDLAKKLKTKCRVGGSAKDGEIIIQGNHKEKVIN
                     MLHEWGYKETKGSGG"
     gene            complement(12963..14015)
                     /locus_tag="PLG24_00820"
     CDS             complement(12963..14015)
                     /locus_tag="PLG24_00820"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HMZ23450.1"
                     /translation="MASLPGRAQRLHLENEIRKIVRLDDDIDLNQGSCTVIGIIDGDS
                     TYTYQWGTLEHQSQSPVTDTSLFVAGGVSHAQLILLTYHMMQLGLINIDDSLGRFQFR
                     EFSGSRLNTFTIGSLLSHNTGLPAILPELTHLKADPDDDYKDYTETAIDSALVVRTKE
                     SGPEGNRPFSHYNQYLLSKVLWAVKDKRVHDNGRMIPEMSHTWFLPLDALKAGSIPAH
                     YTAQQRMLSMPHFNALTPAMGMVTCMDDLVIMTKVLLRLSVQSDFQKTFLDARTTRTK
                     KGIPLSKGGFRMIDGHKSGNIYFMTGLLKGESAFLCFAPKTHTAVIILHNAGVSQHIL
                     GHQIMRMINYQYTRTY"
     gene            complement(14061..15908)
                     /gene="hscA"
                     /locus_tag="PLG24_00825"
     CDS             complement(14061..15908)
                     /gene="hscA"
                     /locus_tag="PLG24_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HMZ23451.1"
                     /translation="MSRVAIDLQSGDIKKEERLILGIDLGTTNSLITYIKDGKPLVLH
                     HKNSTSLVPSVIYLDENHNAIVGSLAKEKLVSDPASTIFSVKRLMGRSFKDVEAHRNF
                     FSYEIVDTDTESLVKIKSGNKFYTPIELSALILKELKRIAEEELGRKVSECVITVPAY
                     FNDSQRQATKDAGKLAGWDVLRIINEPTAAALAYGFDKTSEEGRNIAVYDMGGGTFDL
                     SILTLEGGVFEVLSTKGDTWLGGDDVDKAILDFVIKKGINNVDLDKGQMQQLRLVCET
                     AKKYLADHDIFEGSFDNQQIILTREEYKNLTMPFIKKSLELTQAALKDAGLDAKDIAE
                     VILVGGSTRIPFVKESLSGIFSAPINDKINPDEVVAQGAAIQADILAGNQSHLLLLDV
                     TPLSLGIETVGGLMDVIIPRNSKIPSGAGRHYTTSVDGQVNLRISVFQGERDLVKDNR
                     KLGEFTLRGIPPMPAGLPKIEVVFKVDADGILLVKAREERSNTETQVEIKAQYGISEE
                     EMALMLLDSIKNAKEDVKIKALIEARNEANNIILSTEKFLNQHAELFTDEEFAPLILI
                     ADKLKEVVKGEDKDAIHDIIHQLDKIARPMAEKAMDYSIRQSLAGKSIV"
     gene            16064..16648
                     /locus_tag="PLG24_00830"
     CDS             16064..16648
                     /locus_tag="PLG24_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23452.1"
                     /translation="MACKSDHSGHEEELMEISDDTAGHNVGDLVTTDEILDVPEIHRG
                     RIEQIDGKFVIRATALISATLKEDYNNIDGVMDSYPETFTVTVKKTGDTPSSQKDSVV
                     TMKYKTSYIEFRKRKGMQDRLLIASLQNENFMLQNGLHTGIDRAEALAKFGIREQIPQ
                     DLIEVVSSENNDKILLFLNKDTLVKIEIIPSLKE"
     gene            complement(16660..17289)
                     /locus_tag="PLG24_00835"
     CDS             complement(16660..17289)
                     /locus_tag="PLG24_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ23453.1"
                     /translation="METLAAISRFKANYGFKKGDIIIKEGQISDGVYFIKRGVVKIEK
                     NGPNGRNFIINICGAGNIIGHRGMQGNTIQKSTVVALSEVTCCFIPVNFFNEILKNAS
                     DLEKQITEDYLCQLEQLEKKSISLAYKSVKEKVAEVILVLATIYNYEPKKQSFSIDLS
                     RQDLADLTGATKEQVSSVLKEFERNSIIRYSGKHFYSLDIGTLKTIAEA"
BASE COUNT         4787 a         3480 c         3731 g         5521 t
ORIGIN      
        1 agaatcgttc cggatggcaa ggaggtgacc gtaacacgcg ccagttggga tatggtccgg
       61 tataaacttg acgaaaacga caatggtaaa atcaagcctg aagttgtagg cagctttgaa
      121 cagtttcccc tcaagctggc atgggcaatg tctattcaca aaagccaggg ccagactttc
      181 gacagggtta tcctggatat ggaaggggga gcctttgagt ttggtcaaac ctatgtggct
      241 ttaagtcgat gccgaacact cgaaggcatt gtgctaaaac gcccgcttaa accgcgtgat
      301 ataatggtgg accaacgagt ggtggatttc tatttcgatc atttcagagg ataaaaatag
      361 tcttgtgaat gccgggtcca tgattagggt aaatccatct tatcttcttt agagctttta
      421 atgaatagaa gataggaaac tgcgaaaatg ttccatatga tatttccact tgtattaact
      481 ttatacctat aaaaatttat atttattatg aaattaccgg aactaggcac acataatcct
      541 ttttatcaat tttatttcaa tcttgtccct cagggagatt tgatggagat tttcatgcag
      601 caaatggatg aattttctga gtttatcaga agcattccgg ttgataagct tgattttgct
      661 tatgcgcccg gcaaatggac aattcgtgag gcaatcaatc atgtttcaga cacagagaga
      721 gtatatgcct atcggtctct ggtcgcagcc agaatggata gccagactaa ccttacttca
      781 tttgacgaaa atgcatacgc cttacatgca gatgtatcaa acagatcagt ggatgaacta
      841 attgctgatt ttagggcagt ccggcagagt tctgtttacc tttttaaatt tttaaatgat
      901 acacaggcaa gttttcaggc caaaaatggc aatcacgttt ttacagccaa tggaggcctt
      961 tgttttatga taggtcatgt catacaccat gagaatgtaa tcaaaagcag atatttgaac
     1021 attggggatt aaaacaattt tgttttttgc aggatttggg taaatgtcaa atgatttatt
     1081 ttgagtccag attgagttga tttgattcac gccatctgag tatgatgtga cccgatattt
     1141 ttgacatggt aattgtaaga tttttgaatt taccgtaatg atcaccgggt tatctgaccc
     1201 ggctgatgac ctttcatttg ccctgtttta gtaggttgat cctgccatac cgcatccctg
     1261 aaaagttgta ttttgtattg tttttatcaa ttggtattac aataatggct ggttgatcca
     1321 ttttgtttgt atctttggtc aggatttttt aacaaacttt gataatcaac ccaatctttg
     1381 atacctttta ggagaatatc atctaccata gattattaag cattccacac ttaaagattt
     1441 tgatttttta atattaaact ttaatggcta tgctatcaaa caattcctca tttaaggtct
     1501 cctcgttggt aataggtttc cttctttgta ttttatttcc ttcagatatg ctttcccagc
     1561 aagttgcaat tgacaatgtc aaacctgttg cacgagctgt acaacatgcc aaatccataa
     1621 aagcttttcc aaaagttgat ttgtttgttg aagatctaag tcttgatgta agggataagt
     1681 atccggacat gctttcgcaa ttttcatatt tgaagctcag gaaggaggca ttgaattcct
     1741 ttttgaatac ccggccggat gcaattaata ttcaggtacc ttatttgtca cagaatattc
     1801 agctggagct ggtacgtgta gatgtgcttt ccgatgactt taaaacctat accagcgatt
     1861 ctcctgaaaa ggctgtaaat tattctccgg ggttgtatta taggggaatt gtcgaaggtg
     1921 atgtgtcttc aacagtggca atttcttttt ttgaggatga tgtcatagga atgatatcca
     1981 cagaagagtt gggaaatata acttttaata agatggagga tgcggacaga tatcttattt
     2041 attcagatcg tgacctattg gtcaaaaacc cgaatttttg taacactgaa gagcctgaaa
     2101 attatgcgga acaagtaagg caatatatga ataattcaat agaagtacgg gatggtaaat
     2161 gcgtcaaggt atatattgaa tgtgattatg cattatacca gaacaagggc tcagttacga
     2221 atgttaacaa ctggatttct gctgtatata ataatgttgc aacattatat gctaatgagc
     2281 agatatctac ccaaatttct tctacctttg tttggacttc atcggacccg tatagtaaaa
     2341 caaatagtta taccgccctt acccagttca ggaataatcg gccgagtttt aatggagatc
     2401 ttgctcatct tgcagccctt ggtggacaaa atattggtgg tgtagcatgg gttgatgctc
     2461 tttgtacgtc atacaaatac gcttacagca atatccaggc tacttatcag acagtaccaa
     2521 cctatagctg gacagttgaa gtcatgacac atgagatggg ccataatctt ggctctccac
     2581 atacacagag ttgttcatgg cctggtggag ccttggataa ttgttataca actgaaggtg
     2641 gttgccctcc cggacctcct ccaaccaatg gcggaacaat aatgagttat tgtcatctga
     2701 caagttatgg gatcaatttt aacaatggtt ttggaacgct accgggcaat ttggtcagat
     2761 cccgggttaa tgccgcttca tgccttggaa cttgctcagg tggtggtact tgcaatgcac
     2821 cttccgggct ggttgtcact aatattacaa atacaagcgc agtagtgtca tggaacactg
     2881 tttccggagc aacagcctat caattacagt acagagcaac cggtggttcg tggacagtat
     2941 taagtcaaca gacaaccaca tcagttacac tgaccggatt ggcaggatca actacgtatg
     3001 aaacccaggt gagaagcatt tgttcgggtg gaaattcagc ctacagtgcg attgttacat
     3061 tcaccacgtc agggggtagc ggatgtagtg ttccaacagg ccttgctgcc accaatataa
     3121 caaatacttc agctaccatt agttggaata gtgtcagtgg agcggtcaat tatacactca
     3181 ggtataagct aacctcttcc ggtacctgga atacctatac cacatcctca acaattgtca
     3241 atatgacgaa tatgacaccc ggtacttcct atgatgtcgg agtaaggaca aattgcagta
     3301 acggttcaag taattattcg gctactgtta cttttaccac aaccggaggg ggagcttcct
     3361 attgtacgtc aaaaggcaat acgtcatcct atgaatggat agatttggtt aagttaggtt
     3421 ctatcaataa tgtaacaggt agcaatgggg gatatggaga ttttacagcc cttaatacta
     3481 cattatctgc cggaagttca tacacaatga ccctgagtgc cggaatgaac ggcagctata
     3541 ctgaatattg gactgtttgg atagattata atagggatgg tgattttctg gatccgggtg
     3601 agaaggttac cagcttttct tctagcagta aaggtaacat caacagaagt ttctcagtgc
     3661 caactacagc cagtaatggg cctacaagga tgagggttca aatgaagtac ggatcatatg
     3721 gaacttcctg cgaaactttt agctatggag aggtagaaga ttatacagtt acgatttctg
     3781 gtggcagtaa tcttccatcc gctattacca ataccataac ggattacatg gtgtatccta
     3841 acccttcctc tgatgtattt accgctcagt ttgaggcagg ttcatccatg gaagctacca
     3901 tttcaatcat ggatgccaac ggcagagttg tttctgatca acttgtaaat accattgcag
     3961 gtaaaaatat agtccccttg gagacatcgg gttatcttgt tcccggtatg tatttgatac
     4021 gcatcgtgac aaaatttgga caccaggcta tccgagtcgt taagatgtaa acctgttctt
     4081 tttcggttta atttgaaaga agggaagtcg caagacttcc cttcttttta atctcaggta
     4141 ttctatagag ccggattacc tcaaacactg cttataacag gcagattcag gtggttagcg
     4201 gcatttcttt attgattcct attccagcat cagaatgtta gccatttctg ttctgacact
     4261 cccgattcga taactttatc tacgaatttc attccacgaa tgccttcttc tatgcccgga
     4321 aaatccaggt atttttcaga ttcctgtttg tccgatgcag cgatagtatg aaagaaattc
     4381 ctgtaaagat tggcaaacgc ctcgaggtag ccctcgggat gtccggcagg tgtgcgacta
     4441 ttatgtacag ccattgcccc tgaataccct gctcctgtcc ggaatatttc agccggtttg
     4501 tctggccaca ttaccctgag actgttacaa tctgcctgct gccattccaa accacccttt
     4561 tctccataaa ccctgatacg tagattgttt tcctcaccgg tagcgacctg agttgccatc
     4621 aggactccac tcgccccccc ttcaaaacgc aataatacag ctccgtcatc atccagtaat
     4681 ctgccgggta ctattgtatt tacatctgcg caaatcttat aaacagacaa gccggatacg
     4741 tattccgcca ggttgaaggc atgggtgcct atatccccca tggcaccggc ttttccactt
     4801 tttgtcgggt cagttctcca gcttgcttgt ttattgtttt cctgttcaag cggagcactt
     4861 aaccatcctt ggggatattc gacaaatacc ttacggatac gccccaaaac accagattgt
     4921 atcatttccc tggcttgttt gatcatcgga tatccggtat atacatgggt gagtaaaagc
     4981 tgttttccgg agctctccaa tacctcttta agtcggattg cctcttcggt actgaatgta
     5041 attggtttgt ctattgctac atgaaatcca ttttgcagtg cgagcatggc cggctcaaag
     5101 tgcatatgat tgggggtcac gatacagatg atatcaggcc ggatttctga aggcgatgcc
     5161 gattctttct tgatcatggt tctgaaatcc gtatagattc tgtcctccgg taagaaaaga
     5221 tccttgccgg acgccagggc attttcagga ttggcactca atgcacctgc aacgatttcc
     5281 gccaatccat ccatttgaat ggcaatacga tgtatgctgc cgataaatgc attgcgacct
     5341 ccgcctatca ttgccactct catttttcta aacatattga taataatttt atatactgaa
     5401 atgatatgat aaagaaaatt aaaattttgg ataattgtat tacttttagg ttttactttt
     5461 acaaaaatga aattcaggcc acaattattt atttgaaaca caaattttaa tttaactaaa
     5521 cggaccaatc tctattaatg aagccaactg taagaattca acttaactca atgatgttcc
     5581 tcaacttttt cgtttgggga atttggtttg taacgttggg aacatattta ctcaagggtg
     5641 atataaaagc ttctgacgct caggtggggc ttgcatttgg tacgcagtgt ctgggggcaa
     5701 ccatagctcc ttttatcatt ggtttgatcg ctgataaata ttttgcggca cagaaaattt
     5761 taggcatact ccatcttgca ggtgcagttt tgatgtatct tataagcggg tctaaggaat
     5821 tcactggttt ttatacattg ttattgattt atatggtgat atatatgcct actctggcat
     5881 tatgcaactc aatcgcattg aatcaggtga gtgatgcaga gaaagaattc ggtggtatcc
     5941 ggctatgggg aaccatcggg tggattgtag ccggtcaggt tgtcggcttt ttggcattgg
     6001 agaaaaatcc tggtgacctg gtgatgacat tcaagattgc cggtgctatc tctcttgttt
     6061 tcgggatata tagttttttt ctacctgaca caccaccacc caaagccgga aaagatgttt
     6121 ctttcaggga aattgtcggt ctggacgcgt tgaaagtatt gaagattaag aattatgcag
     6181 ttttcttcat tgcatccatt cttatttgta ttcccctggc attttattat ggtcaggcta
     6241 atatcttcct caatgaaata gggatgcaag gtgctgctca aaaaatgagt cttggccaga
     6301 tgagcgaagc tgtgttttta ttcctgatgc cttggttttt cagacgactt ggagtcaagt
     6361 atatgctgtt ggttggcatg ttggcctggg tggtaaggta tttatgcttt gcatacggtg
     6421 atttggatag tggtctgtgg atgttgtatc tcggaatcat tttgcatgga atctgctatg
     6481 attttttctt tgttaccgga aatatataca cagaccaggt agccggtcct caagtcagat
     6541 catccgctca aggtctcatc acacttgcta cctacggttt ggggatgtat atcggattct
     6601 gggcagccgg ggccatagcc ggtgctaatg tcatcctgga tgctggcggg gaggttacca
     6661 accatcattg ggataaggtc tggttgttcc cggcaatgtt tgcagccggt gtgactgttt
     6721 tatttttatt attttttaaa aaatccgaaa aacaagatat agcgtgaaaa agaatattca
     6781 tggaaggaga gaggtattaa aaaagctttc gcttggagga ctggctttga ccggcgtagg
     6841 gaagttgtct gcttttaata atgactctga agaatctctg gcctggaagg gcaatatacg
     6901 acacagtgtc tgtcaatggt gtgtctcttt tctaaccata gaggagatgg cattatggat
     6961 taaatctatt ggcgtgggag ctatagacct gatcagcccc aaggattgga atattttaaa
     7021 aaaatataac ctcgaatgct ccatgtgcta tatcgatgga aaagtaagcc tgacagaagg
     7081 ctgggctcag cgagagaatc acgcacggct agtggaggat tatattaagt caatacccat
     7141 ggtcaaagat gccggttata ccaacctgat ttgtttcagt gggaatagaa agggtatgga
     7201 tgacgagaca ggattaaaaa actgtgagga aggactgaaa aaaattatga gtttggccga
     7261 aaagaataat gtcatcatcc agatggaact actcaatagc aaagtagatc atcatgacta
     7321 tatggctgat aagacggcat ggggtgtaga gctgtgcaag cgtatcggtt cgccgaattt
     7381 caaactgtta tacgacattt atcatatgca ggtcgatgaa ggagatgtga tccataccat
     7441 tcgtgacaat catcagtata tcggacatta tcatacagca ggtgtaccgg ggagaaatga
     7501 gatagacgat acacaggaat tgtattaccc ggctatcatg aaggctattg ttgaaaccgg
     7561 atttaaaggc tatgttgccc aggaatttat atcaaaggga aaggagaaag acgaaaaatt
     7621 acaggcattc aaacgaggaa ttgaaatatg cgatgtgtga tttgataatg aatcaccttg
     7681 tagtggcctt accatagtag acaaacacac catttatctt ggatataggt attcgtacga
     7741 attgaaaaat ttaaagaact gaattttagt aattctatcg tgaagtccgg aatttgagat
     7801 agacattatt gattagagga cgtcatcacc ggaatcaagt taggtgtccg gaacaagcct
     7861 tctgtttaag aaagcttgtt ccaaaactga tttgtttaat aactcgtgta aggatatact
     7921 tccagagctt tttccagaca tcttaccgca gcttctattt cctctttctt tagtacatag
     7981 gctaacctgg cttctttttt tcccaggcct tttgtggcat agaatccgct caaaggtgcc
     8041 atcattactg tatttccttc aaattcaaag gattccaaca accattgaca gaaatgttcc
     8101 gtatcttcaa ccgggaattc tgcaatacag tagaaagcac cattcggctt cggacaacgc
     8161 actcctttga tcttattaag gccgtccacc aaaagatctc ttcggcctac atattccttg
     8221 attacatcat cgaaataact tttaggtgta tccaaagcag cttcggctgc gatttgttca
     8281 taggttggag gtgaaagtct agcctgcccg aatttcaaag cggcttgcat aatttccttg
     8341 ttctttgata ccagggcacc aattctggca ccacacatgg aatatctttt tgaaaccgaa
     8401 tcgatgagga caacattatt ttcaatcccc tttaggttca tcacagaaaa tggggtggcc
     8461 ccatcgtaac aaaattcacg atatacctca tcagcaaaaa ggaataagtc atgtgattga
     8521 atgatttcac gaagatgttc gagctcttca cgggaatata agtagcctgt gggattgctt
     8581 ggattgcaaa taagaattgc tttggtctta ggtgtgattt tcttttcaaa ttctgatatt
     8641 ggtggcaagg caaaaccatt atcgatactt gcagtaaccg gaacgacatg aataccggcc
     8701 gacacggcaa atccgttgta attggcatag aatggctccg gtattatgat ttcgtcgccc
     8761 ggattaagac aggtcatgaa ggcaaacaag agtgcctctg agcctcctgt ggtcacaatg
     8821 atttcgtcgg aagtgacaga gattccattg gctgaataat aagcagcaag gccctttctg
     8881 taagactcga tacctgcgga atgcgaatac tccaacactt tgatatcagc gtgtttgacc
     8941 gcttccctcg ctatttctgg agtttcgata tcaggttgcc cgatattcag gtggattaca
     9001 tgcttgcctt ggcgtttggc tgtttcggca taaggcacaa gtttcctgat gggtgatggt
     9061 ggcattaagg tagctttttc tgataattta ggcatattta actttgtttt tctatttttt
     9121 cctggagtga ttcgaggatg gctaattgtt tggcgtgact ttcgaataat acctttattt
     9181 gctcctcgag caataaatcg attttctgat gtaatgcctt gatttccatt tcagccttca
     9241 tgttgaccat gtagtcgttg atattttgtt tcctgtcctt ttcttcctgc cggttttggc
     9301 tcatcatgat gatgggagcc tggaaagcag caatacaaga caataataaa ttcatcagga
     9361 taaaagggta ggggtcaaac tgatctgatt tgggcagtgt ataattgagt ataatccaga
     9421 aagtcaatat gatgaaaaaa gtgatgatga aggcccagct tccaccaaat cgggctacct
     9481 tatctgatat tttttgtccg atagtcagta tctcagatgg gggatggagt aagttttcca
     9541 tgatgaggtt ttcttcttca atggatttct gaactatcct gcgtagtttg tcaaggtgta
     9601 tttcctcttt ggaaagtaac tgttcagaat ctttttccat atttatttaa accagaaatt
     9661 aaatttataa aaacaataat caaaaatatg cctattaagg atattatgaa gaatatatcg
     9721 gcaatatttt ctggtttttg ttctttttgt cgtattcact cttgatcgaa aagaaggaaa
     9781 atatggacga aatggttaaa gtcaagccta caacagaggt gaactcatct atttgactat
     9841 ttcccgggaa attggaaaag tgcaacaatg gaaatataaa caggcagaat cccggtaaat
     9901 tggctgctat cgccaatata tgtcgggagg tcttattggt caagtaacag gcaggtcaaa
     9961 gaagattgtc attaaaggaa gaaaaatgac tcttgaggag ttttaactct gcttccattt
    10021 cagtaattcg ttccatgagg ttgaagatga catcgatgcc ttcgaaattg attcccaggt
    10081 catagtgaag ccgtatgatt ttttccaggt tgccgatttg gtctttgttg atataaatat
    10141 cctgatcctt gatgtgatag ctgatgaggc tggtttcatt gagtcgatcc agaaaatctt
    10201 gttccaaacc gtaatgggta cagatggtgc gaattagaat atacttgtca gccattatga
    10261 tctaagtttt ttaagttggc ggaataaatc tttttcctgc tcagagaggt tagtcggatt
    10321 cttgattaga taggttataa taaggtcgcc gaatttttct ttttccttat atacaggaaa
    10381 tcctttgccc ttgagtctta cctttgttcc ggattgcatt tctgcggcta tcttcagttt
    10441 tacctggcca ttaaatgttt ttacggtgac ctcaccccca agtaaagcag tatatagatc
    10501 aatttcgacc tggttgtaga gattggcgcc ttcgcgtctg aaatctgtat cgttatggat
    10561 ttcaaatctt atatacaagt caccatccgg tccgccattg gcgcccggcc cacctttgcc
    10621 agcaatcttt atggtctgtc cgtcttccac acccgccggg atggtcattc tgagcttctt
    10681 gccattgact tctattactt gttgctttga atgatacacg tcagtaaggt tgagatgtaa
    10741 tacggcatta taatcctgcc ctttaaatcg tgtcgaagtc cttgaccttc cacctgaagt
    10801 cctgccaaac atagaatgga aaaaatcaga aaagtcatca tcctcaaagc ctgagtcact
    10861 gaatccactg aacccttcat atcccttacc atagccctga tttccggagg atgctctact
    10921 gccttgttgt ctgcgcatac gttctatttc ttccgcatgc ttccagtcct taccatattt
    10981 atcgtatttc cttctgtttt cttcattgct caggacctca ttggcttcgt tgatttcttt
    11041 aaattttttt tcagcttctt tatcaccagc attcaagtcg ggatgatatt ttcttgccag
    11101 tttgcgataa gccgatttta tttcttgaga tgtcgcggtt ttatctaatc cgagtatttt
    11161 gtaataatcg atatactcca tgaagtaaag tgattttggg ttaaatgatg aaattgttga
    11221 aaataatatc tgtcttatga cttatatcag tgatataaac cacaaaattt acacttttgt
    11281 tgcacattca gctccatatt tcgttgctta gggtgagaat ttctggctcc tgtcgtgtga
    11341 ccaaaatcgt gtgttcatgt tgggcaacaa atccaccctt gtcgcctctg agtgtccatc
    11401 cgtcgttatc cgtttttgta aattcacttt ttgtggaaat aaatgtttcg atcgcgacta
    11461 cttcattttc cctgaatcgc ctgaaattaa atgcttccct gtaattggcg atagagcgtg
    11521 gttcttcgtg aagactgcgt ccgacaccat ggcctgtaag attcttaatg acggtgtatc
    11581 cgaccttctt agctgaatcg tggatataac caccgagttc tgacaccctc ataccgtgtt
    11641 taatacggaa aatagcctgc ttgagtatgt ctctggatgc gtcaatcaga tgatcatacg
    11701 gggagttctg ttgtccaagg gtaaaagagc cgccattgtc tgcccagaat ccatcttgct
    11761 cgatggatac atcgatgttg atgagatcgt cttctcttag gattgtttgg gttgaaggga
    11821 cgccgtgggc tacaacatcg ttgacgctga tacaggtaaa acccggaaag ttgtatgcta
    11881 agataggagc tgatcgtacg ccatattctt tcatcaacct gccaccatac aagtcaatct
    11941 cttttgtcga tagtcccggc ttacaatatt ggcgcatttt cttcaggatt tctccggctg
    12001 tttggccagc cttcctgatt ccatcaagtt cctctttggt tttaataagc attttttagg
    12061 ttacatgttt tgggagatgg tttaacaaaa tatccccggt taaagttatt gggttagtaa
    12121 gattgtatgg tggaatgtta aataatattg tatattcact tcgagacaaa gatatggtta
    12181 ctatgataat gtacggaatt cccaactgca attcagtcaa aaaagccaga gtctggcttg
    12241 agcatcacaa tgttgaatat actttccatg attacaagaa gcttggcatt tcaagcgaaa
    12301 aaatagaggc gtggctcggt caaatacctt tggaaagatt gctgaataag gccggtatga
    12361 catatcggaa ccttaattcc attgagaagg aatcagcatc ggaaaagaat ggtgccattg
    12421 cgatcatgat cgagaagcca tctgtcatca aacgcccatt gatagaggat gaaagcggaa
    12481 aagtgatgtg tctgggcttc agtgatgagt tgtatgagga cttgttcaat ggcaaatgat
    12541 tgcctggcaa ttttaaggaa taatgaccat ttaggttttt tgggaccttt aaatgggaag
    12601 ccatttctta tcctccgctg cccttggttt ccttgtagcc ccattcatgt agcatattga
    12661 taactttttc cttgtgattt ccttgaatga ttatctcgcc atccttggca cttccgccta
    12721 ctctgcactt tgtcttcagt ttctttgcca aatcattgag ctctgactca tttcccctga
    12781 atcctgtaat cagggtgacc tcctttccac ccctctgctt tcggtcgatt gccacccgta
    12841 acttcatcat ggcgggatcg aatccctgtt ccactggttg taagtcggaa aaataattgt
    12901 agttgtaatc aggatctgtg gaatagacga ctccgtctct gttttttttg tttttagaca
    12961 tatcagtaag ttctggtata ttgataattg atcatgcgca tgatttgatg tccgaggatg
    13021 tgctgtgaca cgccggcatt atgaaggatg attactgctg tatgcgtttt gggagcaaag
    13081 caaagaaatg cactttcgcc tttgagtaat ccggtcatga aataaatgtt gccggattta
    13141 tgtccatcaa tcatcctgaa tccgccctta ctcaatggta tccccttctt tgtccttgta
    13201 gtgcgggcat cgagaaaggt tttttgaaag tcagattgga cggataacct cagcaacact
    13261 tttgtcataa tcaccaggtc atccatacac gtcaccatac ccatagcggg tgtcaatgca
    13321 ttgaaatgcg gcattgaaag cattctttgc tgagctgtat aatgtgccgg aatcgacccg
    13381 gccttcaatg catccagggg aaggaaccag gtatggctca tttccgggat catccggcca
    13441 ttgtcatgga ctctcttgtc cttgacggcc caaagtactt tgctgagtaa atattggttg
    13501 taatggctaa atggccgatt cccttccggt ccactttctt tagttctaac tacaagtgca
    13561 gagtctattg cagtctccgt ataatccttg taatcatcgt ccggatcagc tttcaagtgt
    13621 gttaattccg gcaatatggc tggtaagccg gtattatgac tgagtaatga gcctattgta
    13681 aatgtattaa gccggctgcc agaaaattcg cggaattgga atcttcccag cgaatcatca
    13741 atattgatca gtcctagttg catcatgtga tatgtaagta gtatgagttg agcatgactt
    13801 acacctcccg cgacaaataa ggaagtatcc gtaacaggcg attggctttg atgttctaag
    13861 gtgccccact ggtaagtata ggtagaatca ccatcaatga tacctatgac ggtgcaggaa
    13921 ccctgattga ggtcgatatc gtcatcaaga cggactattt tccggatttc gttttctaag
    13981 tgcaaccgtt gtgctcttcc cggaagagat gccataattg tcaaggtgag tagggggact
    14041 aaggtcctga aacacatggg tcaaacgatg gatttgccgg ccaatgattg ccggatggaa
    14101 taatccatag ctttttctgc cataggtctg gctatcttgt ccagctgatg tatgatgtca
    14161 tgaatggcat ccttatcttc gcctttgacc acttccttca gcttgtccgc gataagtatc
    14221 aaaggggcga attcttcatc agtaaataat tctgcgtgct gattcagaaa tttttctgtg
    14281 gataggatga tgttgttggc ttcatttctg gcttctatca atgcttttat tttgacatct
    14341 tcctttgcat ttttgatgct atccagaagc atcaatgcca tttcttcttc acttatgccg
    14401 tattgtgcct tgatttctac ctgtgtttct gtgttggagc gctcttcacg ggccttaacc
    14461 aaaagaattc catcagcatc cactttaaag acgacttcta tttttggcag gccggccggc
    14521 atgggtggaa taccacgaag ggtaaattct ccgagtttgc ggttgtcctt tactaaatct
    14581 ctttcgccct ggaacaccga aatgcggaga ttgacctggc catcgacact ggtggtataa
    14641 tgccgtcctg cacctgaagg tattttggaa ttacggggta ttatgacatc catcaatcca
    14701 ccaacagtct caataccaag ggacagcggc gtcacatcca gtagcaacag atgactttgg
    14761 tttcctgcaa ggatatcagc ctgaatggca gcaccctgag cgactacttc atcgggattg
    14821 atcttgtcat tgatgggggc agaaaatatc ccggacaggc tctctttaac gaaaggtatt
    14881 ctggtagatc ctccgacaag aatgacctct gcaatgtctt ttgcatcaag gcctgcatct
    14941 ttcagtgcgg cttgtgtcag ctccagagat tttttgatga atggcattgt aaggttcttg
    15001 tattcttctc gtgtaagtat aatttgttgg ttatcaaagg acccttcaaa tatgtcatgg
    15061 tcagcaagat actttttagc cgtctcacac accagacgca attgctgcat ttgacccttg
    15121 tccagatcga cattatttat tccttttttt ataacaaagt ccaatattgc cttatccacg
    15181 tcatcaccac ctaaccaggt atcgcctttg gtagatagta cttcgaacac gccaccctca
    15241 agggtgagta tactcaggtc aaaggttcca cctcccatat catacactgc aatatttctt
    15301 ccttcttctg aagttttgtc aaatccataa gcaagagctg cggcagtagg ctcattgata
    15361 atccgaagca catcccagcc tgccagtttt ccggcgtcct tggtggcctg tcgctggctg
    15421 tcattgaaat atgcgggaac cgtaatgacg cattcgctga ccttgcggcc gagctcctct
    15481 tctgcaattc ttttcaactc cttgaggatc aaggcggata attctattgg cgtataaaat
    15541 ttattgccgg atttaatctt gaccaacgat tccgtatcgg tatcaacgat ttcgtaactg
    15601 aaaaaatttc tgtgtgcttc gacatcttta aaagatcgac ccatcaatct cttcacggaa
    15661 aagattgtgc ttgccggatc cgaaacaagt ttttcctttg ccaaactacc aacgattgca
    15721 ttgtggtttt catccaggta aatgacagag ggaaccagtg aagttgaatt cttgtggtgt
    15781 aataccaatg gcttcccatc tttgatatat gtgataaggc tgtttgttgt acccaggtcg
    15841 atacccaaaa tcaaccgttc ttccttttta atatctcctg attgaagatc tatcgctact
    15901 ctactcatgc aacttacaac aataaaaatg gctagatgtt tgtacttaaa tagtaggcgg
    15961 tgaaaaatat cagatttatt caaggggaaa attgtatttt tgcatcacgt aatctttaag
    16021 attaatgtat aaatatttca ttttttgtgg acttatgcta attatggcat gtaagtcgga
    16081 tcattccggt catgaagagg aattgatgga gatcagtgat gacactgcag ggcataatgt
    16141 aggtgacctg gtaacaacag atgaaatact tgatgtgccc gagattcacc ggggaaggat
    16201 agagcaaatt gacggaaagt ttgttatccg tgctacagca ttgatttctg cgactttgaa
    16261 ggaagattat aataacatcg atggtgtaat ggattcttat ccggaaactt tcactgtaac
    16321 agtaaagaaa acaggcgata ctccttccag ccagaaagac tctgtggtca cgatgaaata
    16381 caagacttcc tatattgaat tcagaaagag gaaaggtatg caggataggt tgttgatagc
    16441 aagtcttcag aatgaaaatt tcatgttgca gaatgggctt catacaggta ttgatcgtgc
    16501 tgaggctctt gccaaatttg gaatcagaga gcagattccc caggatttaa tagaagtggt
    16561 aagttctgaa aataatgata agatactttt gttcctcaac aaagatactt tggtcaagat
    16621 agagataatc ccttctctaa aggaataaaa agctataaat tatgcttctg caatcgtctt
    16681 cagggttccg atatctaagg aatagaaatg cttgcctgaa tatctgatga ttgagtttcg
    16741 ttcaaattcc ttgagtacgg atgaaacctg ttcttttgtt gctcctgtca ggtctgccag
    16801 atcctgtctg gacaagtcga tgctgaaact ttgttttttg ggttcataat tatagatagt
    16861 tgccagaaca agtattactt cagccacctt ttccttcaca gacttataag caaggctaat
    16921 tgatttcttt tccagttgtt ccaattggca gagataatct tcggtaatct gtttttcaag
    16981 atcagatgcg tttttaagga tttcattaaa aaaattgacc gggatgaaac aacatgtaac
    17041 ttcacttagg gcgacaactg tgcttttttg aatggtatta ccttgcatac cgcgatgacc
    17101 tatgatatta ccggctccgc atatattaat tatgaaatta cgcccatttg gtccgttttt
    17161 ttcaattttc acaactcctc tcttaataaa atatacccca tcggagattt gaccttcttt
    17221 aatgattata tctcctttct taaaaccgta attggctttg aacctgctaa tggctgccaa
    17281 agtctccaac gaacaaggtt tgagaatcga gcaatgtttt actctacaag tcagacaagt
    17341 attacctgag ggggtcatat tttgacaggt ttatatttta aaaaatgaca ggtcatccat
    17401 acaaagtatg atacaacatt aatgtcatcg gcttaaattt acgaaaatat tcagattgac
    17461 ttattttttt tatacattat ttttgacgta taatttcaaa atgacatatt tggatatgc
//
LOCUS       DAOQQN010000010        17294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1018_length_17294_cov_5.628169, whole genome shotgun sequence.
ACCESSION   DAOQQN010000010 DAOQQN010000000
VERSION     DAOQQN010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 17294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17294
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1018_length_17294_cov_5.628169"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PLG24_00840"
     CDS             <1..840
                     /locus_tag="PLG24_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HMZ23454.1"
                     /translation="TGGERKVTYEDGILLPAYRLPTEAEWEYAALALQGNMLSAKDEI
                     VTDRRVLPWNGDSPRYQKRNKYMGKLMANFKLAGGDYMGVSGNLNDNSSIPSTVRSYF
                     PNDFGLYNMAGNVSEWVADVYRPTNSITLRDVDNQDLNPFRGNEFKQMVVGPDGIPVE
                     KDSLGRLRYELMADSTIADRENFKKADVRSYLDGDNAIDGAYEYGVTSLISDKSRVIK
                     GGSWADRLFWLSPGTRRFKDEDKSDRTIGFRCAMTRMGSSEGNGQNSGNIFGKQKTVK
                     RKY"
     gene            906..2177
                     /gene="murF"
                     /locus_tag="PLG24_00845"
     CDS             906..2177
                     /gene="murF"
                     /locus_tag="PLG24_00845"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HMZ23455.1"
                     /translation="MASLLKYFVEHPSVTIDSRNIKPGQIFFAIKGEHFDGNDFVMQA
                     LQEGAAYCIASNPVFHGHDRIIVVDDTLEALQDLAREYRGTFKFPVLAITGSNGKTTS
                     KELLSAVLSTKFKVHCTYGNLNNHLGVPLTLLATPPDTDFAVVEMGANHQGEIRELCA
                     IAKPDYGCITNIGIAHLEGFGGLDGVRKGKSELFEYLMEHHGTFFINVQENSLDFLND
                     PNNEQLVRISTETEEITILKPTTDFLSFGWYDLNVDTHLVGQYNFNNILIALGVGRYF
                     GCDGRKMCEAISSYVPRNNRSQMIEYRGAQVIMDAYNANPSSVISALDNLIQNPGFNK
                     GLVLGDMLELGEDSAEYHRKILEYIRKADVFSPVMLIGPRFRQFEQAFPEFQFFGDTD
                     EAVSMIDWKSMAGYTVLVKGSRGVRLEKLVN"
     gene            complement(2255..2992)
                     /locus_tag="PLG24_00850"
     CDS             complement(2255..2992)
                     /locus_tag="PLG24_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23456.1"
                     /translation="MEALDIACISPNVQLFHNSSTVCHEVFSEINPRCLENIVNIGIS
                     ATQTHLSSKSDLTPQYFDSYRLGQMIHQMEVINDAIAFADIVVADISAIKQSDIPGRR
                     NISSSGLSSEAFNHIARNAGTGKSRVTVLTGLESLDPGDDIGIDTLAQFVFYFLDGAH
                     SQSSGTDEEILSSYTIDECLPYESVNFLKSEFTGRWFAEFPTRLPDHLKKFQLIPCSY
                     QDYQYSARGELSPRILDIFKRLDELVN"
     gene            3411..5144
                     /locus_tag="PLG24_00855"
     CDS             3411..5144
                     /locus_tag="PLG24_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ23457.1"
                     /translation="MTKKFNLVVLLLAFFGVYTHVDAQSESFKSPVITDEMTVNPDQN
                     REWRAGQSKYSAKPKNMWEVGVHVGRFSISGDIPTTLFPGWGAGIHLRKAINYALSVR
                     VNAMYGTTKGFDDRFSSASIISLEQLPNVGSITNGMYRNYKNTTITGSIEGVLNIGNI
                     LFHKERNKWNLYTAVGVGFASIDTRVNNVKNGAIINWESLNLDPNASYADNVKKLKDA
                     MDSDYETKVGNNRDVSGLFDNGKNALTIPFTVGVSRKLTKRVNLSLEHTYYWADYDAW
                     DGFVYRSAFDQSNDSDNGQYTSLRLGINLGSFDKRTEPLYWLNPLDGALNDVAELKQR
                     PKFDLTDSDGDGVIDMLDQEKNSPSGAAVDTRGVTLDSDKDGIPDYKDDEPYSPPGYE
                     VDSRGVAVIPDKPVTESKLNEILKNTKTDWWLPMIHFDLDKYFVKPEFYPQLQSVASV
                     MKSHPDIKIAVTGYTDVRMGDDYNRVLSYNRAKAAIDYLVGTYNLPRERFVLQYDGKA
                     DPIIPDLPASHNITKKQEMQQYINRRVEFRVATAEDAEMAAPTGPKAGADTPGSSRKG
                     SKYSGNSNSGY"
     gene            5265..6473
                     /locus_tag="PLG24_00860"
     CDS             5265..6473
                     /locus_tag="PLG24_00860"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HMZ23458.1"
                     /translation="MFDNADLSNKKVVVRVDFNVPLDSNLNVTDDTRIRAAIPTIREL
                     HHRGARVILMSHLGRPLKDLNEDGSIKTKYSLKNIVSSLSDSLNLDVIFIATSVQDNA
                     ILTDIENTGDNRIILLENTRFYKGEEKGDPELARKMAALGDYYVNDAFGTAHREHAST
                     ATIARYFDKDHKSFGFLMDKEVSNISRVLDDIQKPFCAILGGAKVSDKIKLIENLLDK
                     ADKIIIGGGMAFTFIKAQGGTIGKSLCEDDKLDLANQLLAKAGEKGVELLLPVDAKVS
                     ETFSNDGLFVTKPIDDIPDGWMGLDIGEASISLFEEAILSSKMILWNGPMGVFEFSNF
                     AHGTFEIASALAKVTKNGAFTLVGGGDSVAAISKAGLDDSVSFVSTGGGAMLEMIEGQ
                     VLPGVAAMQA"
     gene            6495..7646
                     /locus_tag="PLG24_00865"
     CDS             6495..7646
                     /locus_tag="PLG24_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HMZ23459.1"
                     /translation="MVTFSNRLIEIRRHLHKHPELSFQEKETAAYIEQRLIELGVEYT
                     IGWAGYGIVATIKAPVATDHFIALRADIDALPIQETGTMEYASQHPGVMHACGHDVHT
                     TCLLGAIELLIANRERLASDVVCVFQPGEELLPGGASLMMKEGAFDKGLCKAIFALHV
                     FPSMEVGKVGFRAGPYMASSDEIYIRVIGQGGHGAMPHHTADPILAAAGLITSLQSIV
                     SRNCDPVLPCVLTIGKINSEGGATNVIPDSVSLEGTFRTMDENWRSRAHELIGKHCRS
                     IEESFGVRIELNIIKGYPVLVNDEIATQKARSVAVNILGEENVEVLPVRMTSEDFAWY
                     TQEIPGCFFRLGTGNKSRGITSNVHTSTFDVDEDCLALGARLMAEICLQ"
     gene            complement(7653..8834)
                     /locus_tag="PLG24_00870"
     CDS             complement(7653..8834)
                     /locus_tag="PLG24_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HMZ23460.1"
                     /translation="MRTKFPDFTNTEIAFATKSDAELKKMHWLFDKMNRPWLVDLGTR
                     FGLFAIRVNFPFAQTILKNTLFEQFCGGRTLLECVSNVEKLASKNVMTILDYGAEGKD
                     TEDDFNLTMNQIIRAIEFAATQPHINVVSTKLTGLGRFGLLEKMHNKEELTDEENEEF
                     NNLLKRIDSICYNANNHGISVFIDAEETWIQDPVDEIADKMMARYNKTRPVVYNTFQM
                     YRKASLGFLKQSYEKAQKEGYILGAKLVRGAYMEKERKRAADMGYPSPIHDSKADTDT
                     DFDDGITYCIENISGIASCCASHNQKSSEIYARLLIDNNIEGNHPHVMTSQLYGMSDH
                     LTFNLADAGIHSTKFMPYGPVKEVMPYLMRRAQENTSVTGDMSREFELIDREMKRRKR
                     K"
     gene            9110..10243
                     /gene="chrA"
                     /locus_tag="PLG24_00875"
     CDS             9110..10243
                     /gene="chrA"
                     /locus_tag="PLG24_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HMZ23461.1"
                     /translation="MATLFDIFWFFLKAGAFSFGGPNSHIKLMHNELVTKKHLIGEEE
                     FIKIKRLCNTLPGPPSMMLAMKIGYIRKGVLGQLAALIGFILPSSLLMLVIAIYYKEF
                     VNDNFKIIDPFLYGMRTSVIAILLYTTLVYCIRYIRDFKLGAIFLVVAGAALAGLNIV
                     LLLFLSGAAYVLLRQFSSVKKSSLMLILQAQPQFVLPVLDNQKIFWVFVKLGSLILGS
                     GYFVFALIKMDLVDNGLFSINALLDTVAFSLFVPGSIMVAVTFVGYIISSWQGAALAL
                     GGALVPIFVYSLLSSTLLSRLISTASYKLFFRGVLVASLAVLLSIIFQLGYETIGDIR
                     QWVLFILCMLIMFKWPKLNLLWIIIIGANLGYIILHFYPLAID"
     gene            10580..11269
                     /locus_tag="PLG24_00880"
     CDS             10580..11269
                     /locus_tag="PLG24_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HMZ23462.1"
                     /translation="MEEVKPYFKFGLSIDCVIFGYDKGALKILLIKRKEAPFFNTWAL
                     PGNLVNPDEELESAAVRILKEITGLESIYLEQVRTFGEVNRHPIGRVITTAYYSLINI
                     NNFNLVPGSLAERAEWVDIKHVKQLAFDHNNIFNACFNHLKNSIRTKPIGFELLPQKF
                     TLSQLQGLYEAILQQPLDKRNFRKKILSMNILIDNKEQQRHVAHRPAKLFEFDEDKYN
                     NFLREGFTFDL"
     gene            11338..12573
                     /locus_tag="PLG24_00885"
     CDS             11338..12573
                     /locus_tag="PLG24_00885"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMZ23463.1"
                     /translation="MKFDTLDIDERLLEGIDAIGFTDMTPVQEQVIPVILEGRDVIAS
                     AQTGTGKTAAFLLPVIHKLLTNDHDESKINCIIIVPTRELALQIVQTLDGLSYFTDIS
                     SIAVYGGGDGPSFANEKNALSSGVDMVVCTPGRMIAHLNMGHVDLSDVRYLVLDEADR
                     MLDMGFSDDIKKIISKLPQKRQSLMFSATMPHKIRELARTLLSDPFELNIAISKPPEK
                     ITQEAFIVYEAQKMPLIRMVLKSKDFGSIIVFCSKKQHVKVLTHELKRAGFSVDEVHS
                     DLEQTKREQTLLDFKNKKLKILVATDVLSRGIDIEDIDLVVNYDVPNDGEDYVHRIGR
                     TARAASDGTAYTFVSEKEQRKFHEIERLLGKEVTKAVIPEKLGPTPSYNPILAKRSNK
                     KRNFRPRNRRKPGPGQKSS"
     gene            complement(12568..12909)
                     /locus_tag="PLG24_00890"
     CDS             complement(12568..12909)
                     /locus_tag="PLG24_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23464.1"
                     /translation="MEDWELDFHWLKARHQIKDFMNQEKLPDLNMVLLLIGIRELGKV
                     KKKYSKEEKQDLMHLAVCTLLEPEGYYSYKGLDDDGWPHWDIVKPFTLTGVDAQERYL
                     KELIVKYMDSL"
     gene            complement(12911..14170)
                     /locus_tag="PLG24_00895"
     CDS             complement(12911..14170)
                     /locus_tag="PLG24_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ23465.1"
                     /translation="MKTVQSFYLWSKYRVLNISAKLAVRYIFGKKSSNVINIISAIAV
                     FGIAVGTSALILVLSVFNGFEDLISGLFQKFNPDIKITPAEGKTFEPDSSSVYKLQAL
                     NGINRVSATLEELALFDYHNSQTFGIVKGVDTNYAFVTAIRQSIVEGSYNLNGQDPPS
                     VVIGAGVAQRLGVDIEDKLELLRVYTPKRHNRSPMESPFTTRFINPAGIFAIQQEFDQ
                     EYMLSSLDFARELFQYEKEVSALEIRLDSTKNVKNIKTAISKIMGDNFVVKDRFQQDE
                     AFMRLMNIEKWMSYAIVCLTLLLVSFNMIGALWMIVLDKKRDIAILKSIGLGDRGVKA
                     IFLRSGMLLSILGLLIGFVIAVVLYTLQKTVGLVTIPEGFVVTTYPVSLRFIDFMVVG
                     ATVLLIGWLSSIPAARKASRIKTTFSD"
     gene            complement(14270..15574)
                     /locus_tag="PLG24_00900"
     CDS             complement(14270..15574)
                     /locus_tag="PLG24_00900"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HMZ23466.1"
                     /translation="MFTEVTTTIRSEQLMHYLWRTKQLERTNLRLTDGQRLEILDFGN
                     MNTNSGPDFLNGKIRIGNTILAGHIEMHINSSDWYAHGHQNDQAYKNVILHVVCRNDL
                     TVKTPNEVPIPVLLLMDYIDPAIVEKYARLHSDLIGIPCNRLHPEKVSPQVWNIWKER
                     LNIERLQNRVAWVEQEIEISAQDWEKIFYKQLFISMGTNVNKEAFALLADRLDFKIFG
                     RLSDSPDLLHAYVFGMSGMLDQDMDDPYFNCLKSEFKFLRTKYNLHSIPRVVWKYSRM
                     HPQNFPDIRIAQVAELIRANPHLFSTIMECSTTKQLKKLLSVKDLPVYWNFHYRLGGK
                     EGGRKKVMGDSMKNLIIINCIIPVMFIYGKYQNRSELRETALGLLDQLPPEDNYITRM
                     WQELGVPSHSALDSQALIQLKNHYCDRLQCLSCSIGHELLKK"
     gene            complement(15692..16378)
                     /gene="radC"
                     /locus_tag="PLG24_00905"
     CDS             complement(15692..16378)
                     /gene="radC"
                     /locus_tag="PLG24_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HMZ23467.1"
                     /translation="MKPLIIKEMNGEDQPRQKLQTLGSSSLSDAELLAIILRTGNRKL
                     SAIGLAQHVLHSCGGSISNLARKSIADLTAIEGIGPAKAIEIKAALELGKRKIAENNQ
                     LSEVISSSKDAYWAIAPQLMDLNHEEFWVILCNRAHKITDKVCISRGGMHGTVVDMKN
                     LMKIVVQSNAAAIVIAHNHPSGNRSPSREDRNLTTRIKEACHLMDVQLLDHIIVAGQQ
                     YSSFIDEGWL"
     gene            complement(16561..>17294)
                     /locus_tag="PLG24_00910"
     CDS             complement(16561..>17294)
                     /locus_tag="PLG24_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23468.1"
                     /translation="EAINLLDRAIRLNDLDMESYNLLAKVLYNLSLFDFAGQVANTGL
                     QKNPRNPDLIFINMRIAYALKNYTEAAELGTRGMSINSDSSRWNLFTGYAYYYLEDYK
                     SCIPWLLSAVNDNSIGEQGLTLLANSYLNTGNQEKALEYIDIAIELAKDNNLESNLQN
                     KAIILTREGKIREATKLMEQAYALNWNPKILFRHASMQINNKHYASARSLLKKYLASG
                     DAEFKKEATTVLKEIEQTMRTKQKK"
BASE COUNT         4896 a         3364 c         3919 g         5115 t
ORIGIN      
        1 accggaggtg agcgtaaggt tacctatgaa gacggtatcc tgcttcctgc ttaccgactt
       61 ccgacagaag cggaatggga gtacgcagca ctggcattac agggtaatat gctttccgcc
      121 aaggacgaaa tcgttactga tagacgagta ttgccttgga acggtgactc accacgttac
      181 cagaagagaa ataagtacat gggtaagctg atggccaact ttaagctggc aggtggtgat
      241 tacatgggag tttcaggcaa tctgaacgat aactcgtcca ttccatctac tgtaaggtca
      301 tacttcccca atgacttcgg cctttacaat atggctggta atgtttccga atgggtagca
      361 gatgtataca gaccaaccaa ctcaattaca ctaagagatg tggacaatca ggatttgaac
      421 ccattcaggg gtaatgagtt taagcaaatg gttgttggtc ctgacggaat tccggtagag
      481 aaagacagtc tggggcgttt gagatatgag cttatggctg attccacaat cgctgacaga
      541 gaaaacttca aaaaagcgga tgtgcgtagc tatctggatg gtgataatgc tatcgatggt
      601 gcttatgaat atggtgtaac ctctttgata tccgataaat caagagttat caagggtgga
      661 tcttgggcgg atcgtttatt ctggctttct ccgggaacaa gaaggtttaa agatgaagac
      721 aagtcagata ggaccattgg tttccgatgc gctatgacca gaatgggtag ctcagaaggt
      781 aatggtcaaa atagtggcaa catctttgga aagcaaaaga cagtcaagag aaaatattaa
      841 tttttgatta acgttatacg aatcctcaca attaactttg tggggatttt ttttatctta
      901 acataatggc atcattgctg aaatattttg ttgaacatcc atcggtcaca attgacagca
      961 ggaacatcaa accgggtcag attttctttg cgataaaggg cgagcatttc gatggcaatg
     1021 attttgtcat gcaagccttg caagagggag cggcatattg tatagcatct aatccggttt
     1081 ttcatggcca cgaccgaatc atagttgttg atgatacgct tgaggcactt caggatttgg
     1141 cacgagaata tagaggaact ttcaaatttc cggtactggc catcaccggt tccaatggca
     1201 agacgacatc aaaggaacta ttgtcagccg tattgagcac caaattcaaa gtgcattgta
     1261 cgtacggcaa tctgaacaac catctcggtg tgccattgac cctgcttgct actcctccgg
     1321 acacagactt tgcagtcgta gagatggggg ccaatcacca gggggagatc agggaattat
     1381 gcgctattgc aaaacctgat tatggttgta ttaccaatat tgggatagca catctcgaag
     1441 gattcggagg gttggatggc gtcaggaaag gcaaatccga gcttttcgaa tatctgatgg
     1501 agcatcacgg cactttcttt atcaatgtgc aagagaatag cctggatttt ttaaatgacc
     1561 caaacaatga acaacttgtg aggatcagta ctgagacgga agagattacg atacttaagc
     1621 cgacaacgga ctttttgagt ttcggttggt atgatttaaa cgtggatact caccttgtcg
     1681 gccaatataa cttcaataat atcctgatag ccttaggggt aggtcgatat tttggctgtg
     1741 atggcagaaa gatgtgtgaa gctatttcat cctatgttcc cagaaacaat cgttcgcaaa
     1801 tgatagagta tcgaggggcg caggttatca tggatgccta taatgccaat ccgtcatcgg
     1861 taatatccgc ccttgacaat ctgattcaaa acccgggctt taataaagga cttgtattgg
     1921 gcgatatgct tgagctcgga gaagattcgg cagaatatca ccgtaaaata ctcgaatata
     1981 ttcgtaaggc agatgttttt tctccggtca tgctgatagg tccgcgattc aggcaatttg
     2041 aacaagcatt tccggagttt caatttttcg gagacacaga tgaagctgta tccatgattg
     2101 actggaaatc aatggcgggg tatacggtac tggtaaaagg atccaggggg gtacggttgg
     2161 aaaaattggt aaactgagaa agcaatgctt ctctcatggc aaatccttgc tatcctaaat
     2221 catttcatcg gcaatgaaat cctgctgaca acaatcagtt gaccaactca tccagtcgtt
     2281 tgaaaatgtc cagaattctt ggtgagagct ctccgcgtgc tgaatactga taatcctgat
     2341 aactgcaagg aatcagttga aactttttca aatggtcagg aagacgtgtg gggaattcgg
     2401 caaaccaacg tcctgtgaat tcactcttta gaaagttgac actttcatat ggcaggcatt
     2461 cgtcgattgt gtatgaggag agtatttctt catccgtacc agaagattgg gaatgggcac
     2521 catcgagaaa ataaaaaaca aactgggcga gggtatcgat accaatgtca tctcccgggt
     2581 caagactttc cagtccggtc aaaacagtga cacgcgattt gccggtgccg gcatttcggg
     2641 ctatatgatt gaatgcttct gaactaagac cgcttgagga gatattcctt ctgccgggta
     2701 tatcgctttg ttttattgct gaaatgtccg caactactat gtcggcaaat gcaatggcat
     2761 catttatgac ttccatttga tggatcatct ggcctaatcg ataggaatca aagtattgcg
     2821 gtgtaagatc tgacttactg cttaagtgag tttgagtagc agaaatgcct atatttacga
     2881 tattttccag gcacctggga tttatttcgg aaaagacctc atggcagacg gtcgaagagt
     2941 tgtgaaaaag ttggacattt gggcttatgc aggcgatatc gagagcttcc aataaaaatt
     3001 ttaattcgga tagtttgttt tgtaaaataa aaattttaca tttgctattg tttaaaattt
     3061 cactaaacaa agaaaattgc cggtccgttt cctttaatat tcccgggaaa aaacaatggg
     3121 aatatttttg attgttgcgg taggatttaa gcataaccag aattttttca gcaactgaat
     3181 tgtcggttga tagaagtata atgttctgag gggaggaaaa gaattcggaa gtagggcgag
     3241 tatcacacac cttgaaatcg attgaattta aaaagctata gttatcaatc atattggata
     3301 gttgatttat aatttacgcg ttaaagatga atattttttc gcaaatagta aaaaaattat
     3361 acctttgtgg taatatatat aattgatttt taatgttaaa gtaaattcct atgaccaaga
     3421 aatttaacct agtggtatta ctgttggctt tctttggcgt ttatacacat gtagatgctc
     3481 agagtgaatc attcaagtct ccggtgataa ctgatgagat gacagtcaat ccagaccaga
     3541 atagggaatg gagagccggg cagagtaaat attcagctaa gcctaagaat atgtgggaag
     3601 ttggagtcca cgtaggtcgt ttttcgatta gcggagatat tcctactact ctctttccag
     3661 gctggggcgc cggtattcat ctcagaaaag cgattaatta cgcgctttct gtccgagtaa
     3721 acgcaatgta tggaactact aaaggattcg atgacaggtt ctcttctgcc tccatcatta
     3781 gtttggaaca acttccaaat gtaggatcca tcaccaatgg tatgtatcgc aactataaga
     3841 atacaaccat cacaggatcg atagagggag tcctcaatat cggcaacatt cttttccata
     3901 aggagagaaa caagtggaat ctctatactg cggtgggagt gggatttgct tctatagata
     3961 ccagagtgaa taatgtgaag aatggtgcca tcatcaattg ggaatcactc aacctggatc
     4021 ccaatgccag ctatgctgac aacgtaaaga aattaaaaga cgccatggat tccgactatg
     4081 aaacaaaagt tggaaacaat cgtgacgtaa gcgggctatt cgataatggc aagaacgccc
     4141 tcacaatacc tttcacagtg ggtgtttcac gcaaattgac caagagggtt aacttgtcac
     4201 ttgagcatac ttactactgg gctgattatg atgcatggga tggttttgta tatcgttctg
     4261 ccttcgatca gagtaacgat agcgataatg gtcaatacac ttcactacga ttgggaatca
     4321 accttggaag tttcgataag agaacagaac ctctgtactg gttgaatccg cttgatggtg
     4381 cattgaatga tgtggctgaa ttgaagcagc gacctaagtt cgatcttact gatagcgatg
     4441 gagacggtgt aatagatatg ttggatcagg agaagaattc tccttcaggg gcagctgtcg
     4501 atacacgtgg ggttacctta gatagtgata aagatggtat tcctgactat aaggatgatg
     4561 agccatactc acctccgggt tatgaagttg attcaagagg tgttgctgtc attcctgaca
     4621 agccggttac agagtctaag ttgaatgaaa tacttaagaa taccaagacc gactggtggt
     4681 tgcctatgat tcactttgac ctggacaaat attttgtcaa gccggaattc tatcctcaat
     4741 tgcagtcagt ggcaagtgtg atgaaatcac atcctgatat aaaaatagca gtgaccggct
     4801 atacagatgt aaggatgggt gatgattata acagggtatt gtcttacaac agggctaaag
     4861 ccgccattga ctatctggtt ggtacttaca atcttccaag agagcgtttc gtactccagt
     4921 atgatggtaa ggctgatcct atcattccgg atcttccggc ttctcacaat attaccaaga
     4981 agcaggagat gcaacaatac atcaaccgtc gtgtagaatt tagggtagcc acagctgagg
     5041 atgctgaaat ggctgctcca accggaccta aagcaggagc cgacacaccg ggatcttcca
     5101 ggaagggatc aaaatactca ggcaattcaa attccgggta ttaattataa aaatatatat
     5161 tgatgaaaag ggagtcattt ggctcccttt tttattttca aagatttttt caggtaattt
     5221 tttttctgag aataagccat tatctttgga aaaaagttag aactatgttc gataatgcag
     5281 atctatcaaa taagaaagtt gtcgtacggg ttgattttaa tgtccctctg gacagtaatc
     5341 ttaatgtgac agatgatacc agaatcagag ctgccatacc tactatccgg gagctgcacc
     5401 atcggggtgc aagggttatc ttgatgagcc atcttggcag accattgaaa gacctgaatg
     5461 aagatggaag tatcaagaca aagtattcgc ttaaaaacat tgtaagctct ttgtctgata
     5521 gtctgaatct tgatgtgata ttcattgcta catctgtaca ggataacgct atcctgaccg
     5581 atattgaaaa taccggtgac aacaggataa ttttgttaga gaatacgagg ttttataaag
     5641 gggaggaaaa gggcgatcct gaattggccc ggaagatggc cgctttgggg gactactatg
     5701 ttaatgatgc ctttggaaca gctcacagag agcacgccag cactgctacc atagcccgat
     5761 acttcgataa agaccataaa tctttcggat ttttgatgga taaggaagtt agtaatattt
     5821 ccagggtgtt ggatgatatt caaaaaccat tctgtgcaat cctggggggt gcgaaagtat
     5881 cagataaaat taagctgata gaaaacttgc tggataaagc agataagata ataattggtg
     5941 gaggcatggc ttttaccttt attaaggcac agggcggtac aattggaaaa tcattgtgcg
     6001 aagacgacaa attggatctc gcgaaccaac tacttgccaa agcaggtgag aaaggagttg
     6061 aacttctact tccagtcgat gctaaagtat ctgaaacatt tagcaatgat ggtctgttcg
     6121 tcactaaacc tatcgatgat attccggatg gctggatggg cctggatatc ggcgaagcaa
     6181 gtatcagcct ctttgaggag gcaatcctga gcagtaagat gattctctgg aatggcccga
     6241 tgggagtctt tgagttttct aattttgctc atggcacctt cgagattgct tctgcacttg
     6301 ccaaggtaac caaaaatggt gcatttaccc tcgttggggg tggagacagt gtagcagcca
     6361 tttccaaagc aggtttggat gattctgtca gttttgtctc tacaggcggc ggagcgatgt
     6421 tggagatgat tgaaggacag gtattgcccg gtgtggcagc catgcaagca taaacagtta
     6481 tttcgaaaat ggacatggta acgttttcta ataggctgat tgagatcagg cggcacctac
     6541 ataagcatcc tgagttgtct tttcaggaaa aggaaacggc agcttatatt gagcaaagac
     6601 ttatcgaact aggtgttgag tatactatcg gatgggccgg atatggtatt gtagcaacaa
     6661 tcaaagcccc ggttgctact gatcatttta tcgcactcag agcagatatt gacgccttgc
     6721 ctatccagga aacaggaact atggagtatg caagtcagca tccgggtgtg atgcatgcct
     6781 gtgggcatga tgtacatacc acatgccttt tgggggccat tgaacttcta attgcaaata
     6841 gagaaaggct tgcatcggat gtcgtatgtg tatttcaacc cggggaagaa ctattgcctg
     6901 gtggtgcatc attgatgatg aaagaaggtg cctttgataa gggtttgtgt aaggcgattt
     6961 tcgcactcca cgttttcccc agtatggaag ttggcaaggt tggattcagg gccggaccat
     7021 atatggcttc ttcagatgaa atctatatca gggtgatcgg ccagggaggg cacggagcca
     7081 tgccgcatca tacagcagat ccaattttag ctgctgcagg tcttataact tcccttcaat
     7141 ctatcgtaag ccgcaactgc gatccggttt taccctgtgt tctaactatt ggtaagataa
     7201 atagtgaggg tggggcgacc aatgtcattc ccgattcggt atctctcgaa ggtacattca
     7261 ggacgatgga tgaaaattgg cgttcacggg cgcatgaact tataggaaaa cattgcaggt
     7321 ctattgaaga aagctttggg gtaaggatcg agttgaatat catcaaaggt tatcctgtac
     7381 tggtcaatga cgagatcgca actcagaaag cgaggtcggt tgcagttaat atactgggag
     7441 aagagaatgt agaagtgttg cctgtcagga tgacaagtga agattttgcc tggtacacac
     7501 aggaaatacc cggctgtttt tttcgattgg ggactggtaa taaatcacgt ggaataacaa
     7561 gcaatgtcca tacttccact tttgatgtcg atgaagattg tcttgctctc ggtgccagac
     7621 ttatggcaga aatatgcttg caataaaaga aattattttc gtttccttcg tttcatttct
     7681 ctgtcgatca attcgaactc acggctcata tcaccggtca cggaagtatt ttcctgagct
     7741 cgccgcatca ggtaaggcat aacttccttg acaggaccgt aaggcatgaa tttggtcgaa
     7801 tgaatacctg catccgccag attgaaggta agatgatcgc tcatgccgta gagttgactg
     7861 gtcatgacat gtggatggtt gccttcaatg ttgttatcaa tcaacaatcg ggcataaatc
     7921 tcagatgatt tttggttgtg tgatgcgcaa caagatgcaa tgccacttat attttctatg
     7981 caataggtta tgccatcatc gaagtcagtg tcagtatctg cttttgaatc gtgaataggg
     8041 ctgggatagc ccatgtcggc agctcttttt ctttcctttt ccatataagc acctctgacc
     8101 agcttcgctc ccagaatata accttctttt tgagccttct cataggattg ctttaagaaa
     8161 cccagacttg ccttacgata catctggaag gtattgtaga ccacaggccg tgtcttgttg
     8221 tatcgtgcca tcatcttgtc agctatttca tcgacaggat cctgaatcca agtttcttct
     8281 gcatcgataa ataccgaaat tccgtggtta tttgcgttgt aacagataga gtctatcctt
     8341 ttgaggagat tattgaattc ttcgttttcc tcatctgtca attcttcctt gttgtgcatt
     8401 ttctccaaca agccaaatct gcccaatccg gttaatttag tgctaactac attgatgtgt
     8461 ggctgggtgg ctgcaaattc tatggcgcga atgatttgat tcatggtgag gttgaaatca
     8521 tcttcggtat cctttccttc agcgccataa tccagtatgg tcatgacatt ctttgaggcc
     8581 agtttttcta cattagacac acactcaaga agagtcctgc caccacaaaa ctgttcaaac
     8641 aaagtattct taagaatggt ctgagcaaaa gggaaattta ccctgattgc aaacaagccg
     8701 aatctggtac caaggtcaac caaccagggt ctattcattt tgtcaaaaag ccaatgcatt
     8761 tttttcaact cggcatcact cttggtggcg aaagctattt ctgtattggt aaaatcagga
     8821 aatttagtac gcattattcg atttcgatat ttggaatcaa atataatgga ttcgggcaaa
     8881 ttcaatgatt aatttgggat aaatcatcaa ccaatcatga aactcaattt ttttatctat
     8941 aaataaagct ctgttaatga cggaggatag aagaaagaag ccatattatc ttgaaatctg
     9001 ataaatgatc agccaaaaat ctattaaatg cacactcagg tgacttgaaa atggctggga
     9061 ccctatcata ttaaaaatat ttttatatat ttgcaacaag aagcgataga tggcaacact
     9121 ttttgatata ttttggtttt ttctaaaagc cggtgcattc agctttggcg ggccgaattc
     9181 tcacattaag ctgatgcaca atgaattagt tacaaagaag catctcatag gtgaggaaga
     9241 atttatcaaa atcaaaagac tgtgcaacac attaccaggg cccccaagta tgatgcttgc
     9301 catgaagatt gggtacatcc gcaaaggtgt actcgggcaa ctggcagcct tgataggttt
     9361 tatattacca tcttctttac tgatgctggt gattgccatt tactataagg aattcgtcaa
     9421 tgataacttt aagattattg atcctttcct gtatgggatg aggacttctg taatagccat
     9481 attgctctat acaacactgg tatattgtat ccgttatatt cgtgatttta agttgggtgc
     9541 aatatttctt gtagtggccg gtgcggcttt ggctggtctg aacatcgtgc ttttattgtt
     9601 tctcagtgga gctgcctatg ttttgttaag gcaattttca agtgtgaaaa aatcatccct
     9661 gatgctaatt ttgcaggctc aaccacagtt cgtgcttcct gtacttgata atcaaaagat
     9721 attctgggta tttgtcaagt taggttcatt gatcctcgga agtggttatt ttgtatttgc
     9781 attgatcaaa atggatctgg tggacaatgg cctcttctct atcaatgcct tgttggacac
     9841 tgtggctttc agcttgtttg tgccgggatc cattatggtc gcagttacct ttgtgggtta
     9901 tattatcagt agttggcagg gagctgctct ggcattgggc ggtgcattgg ttccgatttt
     9961 tgtttattcc ctgcttagtt cgaccctttt gtcgcgtttg atatcaacgg cttcctataa
    10021 attatttttc agaggtgttc tggtggcctc tctggcagta ttgttatcca ttatatttca
    10081 gctggggtat gaaacgattg gagacatacg tcagtgggtt ttgttcattt tatgcatgct
    10141 tatcatgttt aaatggccga aattgaattt gctgtggata attatcatag gagccaatct
    10201 gggttacatc atactgcatt tctacccact tgccattgac tgatgtggcg gtgattacga
    10261 ccatccggcg gctgattcct actactttag cagtattaat actccggtca atgcttaata
    10321 tatgctatct ttggacggtt ttaacataat attttataaa actattttat tctttgttaa
    10381 ataatttata cttattgtta tttttacacc aagtataaag aataattgag attctttgtg
    10441 cgatttatca ttccgaagtt atcatttttg agagctaaag ggtactgcct tttcatattt
    10501 aggtggcatt gtatagtctt cgatgatggg aggatatttc gtgtatgata tgtaaattaa
    10561 aagatgaaat atttattaaa tggaagaagt gaaaccatat tttaagtttg gcttatcaat
    10621 agactgcgta atattcggat atgacaaggg agcattgaag atattactga tcaagcgaaa
    10681 ggaagcgccg tttttcaata catgggcatt accgggcaac ctggtcaacc ctgacgaaga
    10741 actggagtcg gctgcggtga ggattttgaa ggagattacc ggactggaat cgatctacct
    10801 tgaacaggtg aggacatttg gcgaggtcaa ccgtcatcca attgggaggg ttatcacaac
    10861 agcctattac tccctcatca atatcaataa tttcaatcta gtgcctggat ctctggcgga
    10921 gcgtgcagaa tgggtcgata tcaaacacgt aaagcaactg gcattcgatc ataataatat
    10981 tttcaatgca tgcttcaatc atctaaagaa cagtatcagg accaagccta ttggatttga
    11041 gttgttgcct caaaaattta ccctttctca actgcaagga ctctatgagg caatattgca
    11101 gcagccattg gacaaacgta atttcagaaa aaagatactt tcgatgaata tccttatcga
    11161 taataaggaa caacaaaggc atgtagcgca tcgtccggcc aaactatttg aatttgatga
    11221 ggataaatac aataattttc tcagagaagg gtttaccttt gatctttgat acacaagctc
    11281 tgctctacct gataaggcaa tgctttaatt ccgctaacgg atttaaaatg actggatttg
    11341 aaatttgata ctctggatat tgatgagcgg ttgctcgaag gaattgatgc catcggattc
    11401 acggatatga cgcctgttca ggaacaagtg attcctgtga ttctcgaagg acgtgatgtg
    11461 atcgcttctg cccagaccgg tacggggaag acagcggcct tcctgctgcc cgtcatccat
    11521 aaattactta caaatgatca cgatgaaagc aagatcaatt gcatcatcat tgtaccaacc
    11581 cgtgaattgg cactacaaat cgtccagaca ctggatgggc tgtcttactt tacggatatc
    11641 agcagtattg cagtatatgg tggcggtgat ggaccttcat ttgccaatga aaaaaatgca
    11701 ttgagttcgg gcgtggatat ggttgtatgt acgcccggta ggatgattgc tcacctaaac
    11761 atgggtcatg tcgatctgag tgatgtccgg tatcttgtgc tggatgaggc tgatcgcatg
    11821 cttgatatgg gctttagtga tgatatcaaa aagattatct ccaagttacc ccaaaagagg
    11881 caaagcctta tgttttccgc taccatgcca cataagatca gggaacttgc ccgaacctta
    11941 ttgtccgatc cttttgaact gaatattgcc atatccaagc cgccggaaaa aattacgcag
    12001 gaagcattta ttgtgtatga ggcacagaaa atgccattga tcaggatggt tcttaagtca
    12061 aaagattttg gcagtatcat tgttttttgc agtaaaaagc aacatgtaaa ggtgctgaca
    12121 catgaactga agcgggcggg attttctgtc gatgaagtcc acagtgatct ggaacaaaca
    12181 aaacgagaac aaaccctcct tgatttcaag aataagaagc tcaagatcct tgtcgccacc
    12241 gatgtgttga gcagggggat cgatattgag gatatcgatt tggtggtcaa ttatgatgtg
    12301 cctaatgatg gtgaagacta tgtgcaccgt attggacgaa ctgcacgtgc agcatcagat
    12361 ggcacggcct atacctttgt atcggaaaag gaacaacgga agttccacga aatagagaga
    12421 ctacttggca aggaggttac aaaggcagtc atacctgaaa aattaggtcc gaccccatcc
    12481 tacaatccaa ttcttgcgaa aaggagcaat aaaaagcgaa atttcaggcc tagaaatcga
    12541 agaaaaccag gtccgggaca aaagtcatca tagggaatcc atgtatttga cgatcaattc
    12601 tttcaggtag cgctcctgtg catccactcc ggtcagggta aatggtttta caatatccca
    12661 gtgaggccag ccatcatcat ccaggccttt gtaggaataa tatccttccg gttccagaag
    12721 tgtacatacg gcaaggtgca tcaaatcctg tttttcctcc ttactgtatt ttttcttgac
    12781 tttacccagt tcgcggatac ctatcaaaag taataccata ttgaggtcgg gcaatttttc
    12841 ctgattcata aaatccttaa tctgatgtcg ggctttcaac cagtgaaagt ccagttccca
    12901 atcttccatt ttaatcactg aatgttgttt tgatgcggga ggcttttctt gccgccggta
    12961 tgcttgaaag ccagcctatt aagagcacag tcgcccccac aaccatgaaa tcaataaaac
    13021 gcagggaaac gggataagtt gtgaccacaa aaccttccgg tatggtgacc agacccacag
    13081 tcttttgaag agtataaagc acaactgcga ttacaaaacc aatcaacaag ccgagtatag
    13141 aaagaagcat tccgctcctc aggaatattg cctttactcc tctgtcgcct aatccgattg
    13201 acttaagtat ggcgatgtcc cgttttttgt ccagtacaat catccacagc gcaccaatca
    13261 tgttgaatga taccagcagg agcgttaggc agactatggc atatgacatc catttttcga
    13321 tattcattag tcgcatgaag gcttcatcct gctgaaaccg atctttgaca acaaaattat
    13381 cgcccattat cttgcttata gcggtcttta tattttttac attttttgtt gaatccaatc
    13441 tgatttccag cgctgaaacc tctttttcat actggaataa ttcacgggca aaatccagag
    13501 acgaaagcat atattcctgg tcaaactcct gttgtatggc gaaaatcccg gccggattaa
    13561 taaatcgtgt tgtaaatggt gattccatgg gtgagcggtt gtgcctcttc ggggtataca
    13621 ccctgagcaa ttcgagcttg tcctcgatat ccacgccaag tcgttgtgct acgccggctc
    13681 caataaccac ggatggggga tcctgaccgt tcaggttgta agatccttca acgatgcttt
    13741 ggcgaatagc agtgacaaaa gcataatttg tatctacccc ttttactata ccgaaggtct
    13801 ggctgttgtg atagtcaaac aaggctaact cctcaagtgt cgcacttacc ctgttgatac
    13861 cattcagagc ttgtagcttg tagacgctgc tgctgtccgg ttcgaaggtt ttgccctcag
    13921 caggagttat cttgatatcc ggattgaatt tttggaacaa tccagaaatc agatcctcaa
    13981 atccattgaa tacggacaag acaaggataa gggcagaagt acccacagcg atgccgaata
    14041 cagcaattgc tgaaatgata ttgatgacat tggaggattt ttttccaaag atgtatctga
    14101 cggccagttt tgcagaaatg ttcaataccc ggtatttgct ccaaagataa aatgattgga
    14161 cggttttcaa agggagaaat tatttcaaag tgatagggat ctctctgaga gggccttatc
    14221 aaaagccatc ttatggtaca atcatctatg atgagtgagg gctctgatac tattttttta
    14281 atagttcgtg accgatgctg caactcagac actgcaatcg gtcacaataa tgatttttca
    14341 gctgtatcaa agcctgtgag tcaagagccg agtgacttgg cacgccaagt tcctgccaca
    14401 ttcgggtgat atagttatcc tcggggggca attggtccag cagtcctaaa gccgtttccc
    14461 ggagctcact tctattctga tatttcccat aaataaacat tacggggatg atacaattga
    14521 taataatcag atttttcatc gagtcgccca tgactttttt ccggcctcct tccttgccgc
    14581 ccaacctgta atggaaattc caatacaccg gtaaatcttt gacggaaagc aattttttca
    14641 attgttttgt agtcgaacat tccattatgg ttgaaaagag atgtggatta gccctgatta
    14701 attcagcgac ttgtgctatt ctgatatccg ggaaattctg tgggtgcatt cgggaatatt
    14761 tccatacaac tctggggata gaatgtagat tgtatttggt tcgcagaaat ttgaattccg
    14821 atttcaggca attaaaataa ggatcatcca tatcttgatc caacatgccg gacatgccaa
    14881 atacataggc atgaagcagg tccggagaat cggacaatct tccaaatatt ttgaaatcca
    14941 atcggtctgc cagcaaagca aaggcttcct tattgacatt ggtgcccata cttataaaca
    15001 attgcttata aaaaatcttt tcccaatcct gtgctgatat ttcaatttcc tgctctaccc
    15061 aggcaacgcg gttttggagc ctttctatat tgagtcgttc cttccagatg ttccatacct
    15121 gtggactgac tttttccgga tgaagtcggt tgcacggaat tccaataaga tccgagtgca
    15181 ggcgagcgta tttttcaaca atagccgggt caatatagtc catgagcagc agtaccggaa
    15241 tcggtacttc attaggagtc tttacggtca ggtcattgcg gcagaccaca tgaaggatta
    15301 cgtttttgta agcctgatcg ttctgatggc catgggcata ccagtccgat gagttgatgt
    15361 gcatttcgat atgaccggcc aggatagtat tgcctattcg tatctttcca ttgagaaagt
    15421 cagggccact gttggtattc atattcccaa agtccagaat ttctagcctt tggccatcgg
    15481 taagcctcag attggttcgc tccaattgtt tggttcgcca caaataatgc atcaattgct
    15541 cggagcgaat tgtggtggta acttcagtga acataatgag aaatttataa aattaaaaaa
    15601 tgataattga ttggtgatat atgcatggat cgaacagaaa agatgcttca aacccaatat
    15661 tacaaaccgg caatagactg gaatctcctc ttcaaagcca cccctcatcg atgaaactag
    15721 agtactgctg tccggcaacg atgatatggt ctagcagctg aacatccata agatggcatg
    15781 cctccttaat cctggtggtc aggttcctgt cttcccgact ggggcttcta ttgccactcg
    15841 ggtggttgtg ggcgatgacg atagcggcag cattggattg gactacaatc ttcatgagat
    15901 ttttcatgtc aacaacggtt ccatgcattc ctccccttga tatacacacc ttatcagtta
    15961 ttttatgtgc cctgttgcac agtattaccc aaaattcctc gtgattgaga tccattagtt
    16021 gcggagctat ggcccagtag gcatcttttg aagatgaaat gacttcagaa agttggttgt
    16081 tttctgctat ttttcgttta ccgagctcca gggcggcttt gatttctatg gcttttgctg
    16141 gcccgatacc ttcgatggcg gttaggtcgg caatgctttt gcgggccaga ttgctgatgc
    16201 ttccaccaca actatggagg acgtgttgtg ccaggccgat ggcagaaagt ttccgattgc
    16261 ctgtcctgag aatgatggca agtagttcag cgtcacttaa tgaagatgag ccgagtgttt
    16321 gaagtttttg gcggggttga tcttccccat tcatttcttt gatgatgagt ggtttcatat
    16381 ggatgatttt gtgcacgcta agttaatact ctctctcgga atggaaaata tatttgtgga
    16441 ttaatttttc agaaaggata atatcagccg gagatatcgt taaattaaaa gtactaaaag
    16501 agcaatgtta aagacctgtt gtaattcgga taccgtaact tcagattaat cgggcactct
    16561 ttattttttt tgttttgtac gcatagtttg ttctatttct ttcaaaacag ttgtggcttc
    16621 ttttttgaat tccgcatcac cacttgctaa atattttttt aacaaagagc gtgcagaggc
    16681 ataatgcttg ttgttgattt gcatggaagc atgacgaaac aatatcttag gattccaatt
    16741 cagggcgtat gcttgttcca tgagctttgt tgcttcacga atttttcctt ccctggttaa
    16801 gatgattgcc ttgttctgga gattgctttc cagattgttg tcctttgcca gctcgattgc
    16861 gatgtcgatg tattccaggg ctttttcctg atttccggta tttaggtagg agttggccaa
    16921 taatgtcagg ccttgctcgc caatggaatt gtcattgact gcacttaaca accagggaat
    16981 acatgactta tagtcttcga gataataata ggcatatcct gtaaatagat tccaccggct
    17041 tgaatcactg ttgatactca tacctcgggt acctagttct gccgcttctg tgtagttctt
    17101 cagagcataa gcaatacgca tattgataaa gattaagtca ggattgcgtg gatttttctg
    17161 tagcccggta ttagccacct gtccggcaaa atcaaacaag gataagttat acaatacttt
    17221 agcaagaaga ttataacttt ccatgtcaag gtcattgagc cggattgccc gatcaagtaa
    17281 attgatagct tccg
//
LOCUS       DAOQQN010000011        16535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1089_length_16535_cov_6.535680, whole genome shotgun sequence.
ACCESSION   DAOQQN010000011 DAOQQN010000000
VERSION     DAOQQN010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 16535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16535
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1089_length_16535_cov_6.535680"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2130)
                     /locus_tag="PLG24_00915"
     CDS             complement(<1..2130)
                     /locus_tag="PLG24_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HMZ23469.1"
                     /translation="MWTFSTSNNAQTFVPVNVTGFNHDLIANGSGGANRAEATTTITF
                     DDPANGTTSDNVLYSKNFRGNSNPSTIPPFGLPDDGIITSLNLPGAVYHLASYDQNNA
                     LVLRTQGSTGTLTLETPGVFSKIAFLGASSNGNSSFDFVLNFSDGTSFSTTFTVPDWY
                     SGSNYAIKSIGRVYRTTIGSHLADEFNGDAENPRLYDNELIIDPPYNSKILSSITITK
                     TTSTGSTGIFAINGITAINAPDAPIANVATDITINSFTANWALTSNTTEYYLDVSSSP
                     TFSTLLPGYNNLNVGNVQSLSVTGLSNGETYYYRVRAGNVAGISASSNTIEAALIQCP
                     SGDLFFTTQAQVDQFIIDYPKCTEIPGAVLVQAPDATDLASLTNITKIGGNLDIWNCP
                     ALNKLTGLTKLTQLGGRLVVFGCGSLTNISPLANLTSINGIISLDGNVQLNDITGILN
                     ISLGSITELYIQNNLSLSVCDYSLVCDFLKTAKPRNIANNAGECITEQSVKDACNNTS
                     LIDGEYCSNAIDINTLFGHPIGEPHASGTYSTEGMNNLNDPAFGHECFNNDLNTIWFK
                     FVGDGNRYAVRSNDCSAAVYKNPNGAVYAGTCDTLSAIKCHRDIWTGDIDTDINFKIE
                     LNTEVGKTYYIMVEITTSNDLIQFLNYGEFCMEVTRLDEECLVTIPDANLKAYLIANA
                     DINKNGDTEIQCEEAKQFVGKINCSDLN"
     gene            complement(2208..4364)
                     /locus_tag="PLG24_00920"
     CDS             complement(2208..4364)
                     /locus_tag="PLG24_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23470.1"
                     /translation="MKNISLILIIVFFQGLVLNQAIAFGHISDLEPHLKSHKIKQNQS
                     SFDTLPVHNNDDKIIGMAKAYEDSIVLRMSPPYYLFWESVKQNGFTIKRGTSKDNLKE
                     ISKVFPYPMESSEPKLREMDSISQVAYFLLYGNIDRGDLQGYKLYQADQNILGMAMLV
                     SEFSKDAANILGFRYVDRNVSKGQKYVYEISTAGFKDSKYNEVIELVNTLQAIKEPVA
                     FQVLDGDNTLTLQWSKDNVENFTYYKIERSTDQKNFEPITSRPLFFNSTNDTLTNNFI
                     YKDSLDIINGTTYYYRLYGGNSFAEYSPAAFASGTPRDLTPPVPPKITKVTFDDKLIQ
                     FDVEWDIEIATVPADFAYYQVMASRDERVSYTAVSPKLGLADFNYTYDLGTEWKEANE
                     GRYYFRLECYDKNGNKSSSDFETSFVPDYTNPAAPDTLSGYVDSLSFVHLKWDKSTSK
                     DVRGYWLYWGNSTQEELALVSQDILTDTSYQYYIPEKSLKKNMYYVVRAEDHAYNRSD
                     ISNIAIVKLLDKVPPIRPSIKSIQNDSLRLQLNLELSPSDDVYFHELYRRETMSKDTS
                     WQLLDTLWNIDSDTYIDQIAELNKSYQYKIRAVDSVANKSIFSPLKSGVIKVTAKNTG
                     ISQLSIKQKEKSKAVEVSWQVEMPKKLSTTAFTVEIYRSSGQDGIKHYKKMDKDLDVF
                     TDNDLQSGVLYNYAVRVRYENGWTSELSEIKSILIK"
     gene            complement(4490..14269)
                     /locus_tag="PLG24_00925"
     CDS             complement(4490..14269)
                     /locus_tag="PLG24_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23471.1"
                     /translation="MNIRQYLFSIIFSTVSIFMLQAQTEVWPVQVSGSMLPPYSLDLK
                     VYSLERAEDLSFTAILKDPVEANLLVKPVITVEQNGTVIYQTDPNYAGKTILLSQFNP
                     IILNGSALGEYLSNVALTGAQPGSKGSVEMPEGFNQVCLQLYGVERNVPVSNKFCISG
                     NFRLCQPPQISKPTFNEKIKMPEVQNYIFSWQPMHIGSPNNPGPVEYTFELVELPVGV
                     MNANDVFASALKVYTTTTMATSLIYTQGEPTLEPNKYYAWRVTATSVMYPTSKLFQND
                     GKGEVSIFILYEGEVPAGDVNPFDKASPRGCSVYETSYGAVSKADNVPVIASPNQNVK
                     VGYFDMKITEAYSSQTQSYSGKGLVEYPMLRSVLEVEFKNIKVNKEGRVYEAESISAL
                     IDPALQFNLYNLSKESIEKAITSKYIDKLNTLLQKPKSDVAELAEGSTVFNSLPVSLH
                     FEKQQPPVAVIGMVLTPTNAFINLLSQTDDGGIYVATLIPTTPFGVKNNAYLIPIHAD
                     GIERSSVKLLESIESGNVVSDKSKMRCNCKGFESLNDQVQLSISPDIMTRTDNGAPVT
                     FEPTKETGNYDNKITEVENIPRFKLNNLPGFTFQAKSGKLDLSSSENGFNKDVNDYTA
                     KQSINWKGLRLKDIQLTIPEKYNIVDPNSPLTLDNGEIYIDENKLAYGHFSKTNVLGL
                     DKGKMGPWNYSIDTMKVTMDAHQIGKFRLSGNIKTPFFDDHFPYRAVVAETRQKQIQL
                     DAIIPSSKLGMSIWHGSFTTQKPSSVDARLVQLGEERQLTPKCDFTGDLNVNFTDKEF
                     RAAILNTNKGETLDALKNALKIDNFAFELSGLWLDGLSNDPMNEREKRFKIEGFDLKN
                     VSLQIGGTSHKLSSASLKYVTEEKRERLGLKTIVVKGSSKVELTIWAAADKGTFRFEG
                     IEVNNIDLKCNCGVASVIPSKDEWDRIIDDFYEKHYILSAAGFTQSGTQSVFPEKLFY
                     IGKELAIEKIKMNTIAWFPTIDNNSAINIPFLDRNLSIENIGGAYTGKYRNGVAVNSK
                     TEWTKEVFNSLANESTGDLHLPLIITEELWSKFGFKGDYTLPENYKLFITEFKTAGSD
                     NLSNATVKIGLVGALMVEEKEVFVYFESNEDIAIGPDKISLKDILLHLTKDAKLDENV
                     TYLSSGNGDKTAETGSYVGLDCTAGIHNFNLQGHYTMPSETLIGYDETGAVTGPAVFG
                     FRLIENSIDGNGALLTEFKAPLKTTYKSAKGWQEWSFSTDGDRHIVFNGGKSLEAYLD
                     YSSTTAVDRPQGEIDDLTRERLYNSYFRGIIFNQIGFSIPILEQNRVDAKSNPEFFKD
                     TVSYSFYEIDSKAFFSKYQGFHKVKKENLARLGGWQYHLDTIRFNIEYNQLDADKLTL
                     KGATLIPLFRSAPADNKAKWEETFTDAWVPYSLSIEYDAKEQIPAISGFVDGVNESLF
                     ELNYIHGMGMNLDKESFVDFGYDKDRKITTAKASLNGRAIYFIEKLDAKIPFLKFQNF
                     NVNHNIIKKNNGSDMCSSNDIAGIESIDFGTWSIMYFTEVEKEAISKAANSSTGQNLS
                     GKIKESKFAKGLGADISSFSKMVGFDISIHEPSFFCTGSEWKMVIGLDLSIMRDKSNL
                     TASQQEVSDKFNPKEAAERKKAEEENSLKPLEKDYKEAQNNIKTIADERSKLLAEKQR
                     LETELKTFKKDKNIKINGNNVSYNSASNKSEIDSRNSTIASLNTKITAANTKYKTALT
                     SVKEKYKPFKEQSAKLKTASNELDKQTKAVVERTEKYKNAGAGERLEMDKEKFKKDLA
                     EAKATKTGAFSASGDIEVSFDGKGYKDVALTCLGLGGEFGPVKFKGGINLFRDETTKG
                     NYDPNAVQSAWGNGFLGMIDVSILDYNFATKFQTGVKLDGATAQAAQDFRYWFADLSF
                     SANPGLPLGQSTFSLTGIGGGFYYNMAKEIPGLDQIKKQSPPPVANNDLCKAEGMEAG
                     KSLSGLKYTVSRGSMGGYLSAEISHPARVSLEGILSLEIAIINDEFKFKNFGLGLNGY
                     ALYEDFATRKTESPLIVKGDINLNFEENIKVIGGIDFRFGKKAGPLEIAAPSDKNSNS
                     NSWNSIRFCFSNEFNYVHAGSWGIPGRLGSGPSSGLNFLSAGFEAPLFGKVSAGLYAQ
                     LGNKTDGQPKISYLLPNYKGTDNSLESSSILSDRFAANAGFRLSADMGSEFLIFKWYA
                     TAEIGANLAIQNWGKGVTCNGKKEKVGFENGYYLTGNAYASLKATASIDTWVKEFEIF
                     DGSVDLVLDFGFPNPSYLLGDFAVKYNVLGGSYAGTQNMEIDLGEKPCDDYQADPLVG
                     IKAHHDFEFFENKENGDNVFVHVKVKNNVVLEKNFNVRKKGNDGKTLKCSLDVTLKEK
                     SSGKIIPLVDRLYMKDKLYDVCMLPYSLMKPNKSYIIEAKYTWTSAPFTMQNGVYQAT
                     GYAAKIAEEVVTKEFKTSPLWGIKVHDGIFFNDDPVDLEGGDILYQKLDSLTVLHQFD
                     FSKEQLLETEDNSKEYLGCDIDVSLINKYSGDEIARLDKINEKVYNGDISKSVSIFKL
                     NQQLKAGGMRYTAKVTYHFRTYNKTATGEEINIKKMDDQIFTRTFEPSPYYEIKAHND
                     FVFPENGGFINKINTFKIRHNMAPYQGHLGLSNLIIRNKATGKVVRIVQNFISNNLNN
                     DILYFEPDFLLQPNTVYSMEARYIERAGNPLIDHSKETVFGEFATSPAYNIDIHNSLV
                     LGSQNDRFEGGFKNLSSYKEDKGIITVWLQQDSLKIASNINMDEVFKIKLANKVKYIK
                     CDLEVKLEDSISGKTISLLKGSSTKSYLGFMNNYYLTQKLEANKAYLIKAKYTLYSYE
                     TTEAGQMFNKQSIKEELANATFITSQFYGSPAFKGLEVNLNKTGKYGPDFAVLKVSHP
                     IDRNIELGDGSSMEITPKLTVINKLTNETISFVSPYSSDTENYPLEFSNLNLNTTYIL
                     KGNFLWNYVDKKGGHMHPIVEPVYVEFVPKDLDKTTNDVALKTKFDDADIIGNGGIDD
                     PINNNLVTTNFNQIKIKHKTDIEKERLVLSENNTPKFMKCDLIVSIKEKLSGVAVPVN
                     KIRYEDGTGYNNGLFFSIYKISQELKLNTTYLLEVKYNWMQYDKSETGVVSNEKIVRV
                     ESESSEFTTVDKPKETIHNEFIFPKEGETISSKSTEIKFRHNAAMNKEIRLDEFNSVR
                     YSLSSIEINQRGTANRIKLGKPTTGENGLVTVCPINAPGLKPNTTYNLLVKYDKLLFS
                     RDSGGISSIPGKPEIHNIIFKTGDE"
     gene            complement(14320..16164)
                     /locus_tag="PLG24_00930"
     CDS             complement(14320..16164)
                     /locus_tag="PLG24_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23472.1"
                     /translation="MSIRNLFLVFITLFCLTHNKMYGQLNSFENLEQYNEIGLFKSSL
                     KGFFKGFADFGEPFQLGGGIGIGLRSYNAVGGPLRQDPFFYTINGHLNVRIYQIELPL
                     SVVMTAKNTTSSYPNFSDLKESLKQNLRDKANSFARIGMSPYYKWAKIHLGHRAMNFS
                     KYTMSNLNFFGAGLELNPGKIRIGGMYGRLAKAEPIDLSLTTPNLPVYQRIGWAAKLG
                     YGDDKASADLTLFTARDDENSISIPANYPEQVTPEANLALGVTLQKLFFDLVRIKVDY
                     GNSTISPNALDADSPDTGITNFLLKKKNTTYVAHAIEGSIVLETKTFNAGVIVNRVDS
                     NYKTLGTYFFNRDIMDISGFTQFGLLDGKVNASLKAGIQSNNLDKSLPTTNKRLIYDA
                     QLTYAIPKVSASFNYSNNTARVNYLLNQNLDSLNAVIVTRDMGLNIQYNLPTTNEISH
                     AISATGNIQDVSDDIEKPSRTLTSKVLLGSLSYMLRLPSDWNFSLRGNYTQNEISGLQ
                     TDRVGYGLGVRKAFFENKLTIGVDGNFYNNKNALGQKSKNAIGQFMVAYQLFKGMGIN
                     LQWGLLNTTTGTGPAFTESIGNLTMQYQFSYVPKKKSKQEKEKNNKAE"
BASE COUNT         4613 a         3405 c         2987 g         5530 t
ORIGIN      
        1 gttgaggtca gaacaattga ttttacctac aaattgttta gcttcctcgc attgtatttc
       61 agtatctcca tttttattga tatcagcatt tgcaatcaga taggctttca gattagcatc
      121 tggtatagtc actaagcatt cttcatctaa tctggtaacc tccatacaaa actcaccata
      181 gtttaaaaac tgaataagat catttgatgt agtgatttca accataatgt agtaagtttt
      241 gcctacctcg gtattcagtt caatcttgaa attaatatcc gtgtcaatat ctcctgtcca
      301 gatatcacga tggcatttta tagctgacaa tgtatcacat gtgccagcat acacggcgcc
      361 attcggattc ttgtacactg ccgcactgca atcatttgac cgcacagcat atcggttacc
      421 atctcctaca aatttgaacc atatcgtatt gagatcattg ttgaagcatt cgtgtccgaa
      481 ggccggatca ttcaggttgt tcattccttc tgtgctatag gtacctgaag catgaggttc
      541 gccaatcgga tgaccaaaaa gagtattaat gtctatggca ttagaacagt attcaccgtc
      601 aatcaaactc gtgttgttgc aagcatcttt tacagattgt tcggttatac attcgccagc
      661 attattcgca atattcctag gcttggctgt ttttaaaaaa tcacacacaa ggctataatc
      721 acatacagac aaagacaaat tattctgaat gtatagttct gtaatacttc ctagagatat
      781 gttaagaatg cctgtaatgt cattcaattg tacattccca tctaagctga taataccatt
      841 gatggaggtc aggttggcca agggacttat atttgtcagt gatccacaac caaaaacgac
      901 caatcttcca cccaactggg taagcttggt caatcctgtc agcttgttaa gcgcagggca
      961 attccaaata tctaaattgc cacctatctt ggttatattg gtcagagagg ctaagtctgt
     1021 tgcatcaggt gcctggacaa ggacagctcc cggtatttca gtacatttag gataatcaat
     1081 gatgaattgg tcaacctgtg cttgtgtcgt aaagaatagg tcacctgatg ggcattgtat
     1141 caaggcagct tcgatggtat tgctgctggc actgatacct gctacattcc cagccctcac
     1201 cctgtagtag taggtctcac cattacttaa ccctgtgaca cttaaactct gtacattacc
     1261 tacattcagg ttattataac ccggcaacaa cgtgctgaaa gtagggctgg aagagacatc
     1321 caaataatat tctgtcgtat tggatgttag tgcccagttg gcggtaaatg agtttatggt
     1381 gatatctgtg gcaacattgg caataggggc atccggcgca ttgatggcag tgatgccgtt
     1441 gatggcgaag attccggtac tgccggtaga ggttgttttg gtaatggtga tgctcgatag
     1501 tatttttgaa ttgtaaggag ggtcaatgat taactcatta tcgtaaaggc gaggattctc
     1561 agcatctcca ttgaattcat ctgccaaatg gctgccaatt gtagttctat aaaccctacc
     1621 tatgctttta atggcataat tagaaccaga ataccaatcc ggtacagtga aggtagtaga
     1681 aaacgaggtg ccatcgctaa aattcaatac aaaatcaaaa gatgagttcc catttgagct
     1741 ggcgcctaaa aaggctattt tggagaatac cccgggagtt tccaaagtta atgttccagt
     1801 actgccttgt gtacgaagca ccagagcatt attttgatca taagatgcca gatgatatac
     1861 cgctccagga agatttaggc tcgttattat accgtcatcc gggaggccaa atggtggaat
     1921 agtggacgga ttggaattgc cccggaaatt tttggaatac aacacattgt cgcttgtcgt
     1981 tccatttgca ggatcatcaa aggtaatggt ggtcgtggct tccgccctgt tagcccctcc
     2041 ggatccattg gctatgaggt catgattaaa tccggtgaca ttgaccggca cgaaggtctg
     2101 ggcattattg ctggttgaaa atgtccacat aagtatgcac aatataagta ttcggaacgt
     2161 atatattctg ttcataacta ctattctttg tgttttattg aataatatta tttaatgagg
     2221 atggatttga tttcacttag ttcgctcgtc cagccgtttt cgtagcgaac tcggacggcg
     2281 tagttgtaga ggacgccgga ctggaggtca ttgtcggtga atacatcaag atctttatcc
     2341 atttttttat agtgtttgat accatcctga ccggatgacc gatagatttc aactgtaaat
     2401 gcagttgtag aaagtttttt cggcatttcg acttgccagg atacttccac ggctttggat
     2461 ttttcttttt gctttattga aagttggctt atgcctgtat tttttgctgt tactttaatg
     2521 actccgctct taagtggaga aaagatactt ttattcgcta ctgaatcaac agcgcggatt
     2581 ttgtactgat atgacttatt taattcagca atttgatcaa tataagtatc cgaatcaata
     2641 ttccatagtg tgtccagcaa ttgccagctg gtatcttttg acatagtttc ccggcggtac
     2701 agctcatgaa aatatacatc atcactaggg ctcaattcca gatttaattg cagacgtagc
     2761 gaatcattct ggatgctttt gatggagggt ctgatgggcg gtaccttatc caataatttt
     2821 actatggcaa tattggagat atcacttcta ttgtaggcat gatcttctgc cctgaccaca
     2881 tagtacatgt ttttcttcaa tgatttttcg ggtatatagt attggtagct tgtatccgta
     2941 aggatgtcct gactcacaag tgcaagctct tcttgggtgc tattgcccca atatagccaa
     3001 tacccacgta catccttgga ggtactttta tcccatttta gatgcacaaa actcaatgag
     3061 tcaacatacc cggacaatgt atcaggtgct gcaggattgg tatagtccgg tacaaaagaa
     3121 gtttcaaaat cactggagct tttgtttccg ttcttgtcat agcactccag ccggaaataa
     3181 tacctacctt cgttcgcttc tttccattct gtccctaggt cataagtgta attaaaatcc
     3241 gccaagccca atttagggct tacggcagta taagatactc tttcatcgcg agatgccatc
     3301 acttgataat acgcaaaatc cgcaggtaca gtcgctatct ctatgtccca ctctacatca
     3361 aactgaataa gcttatcatc aaatgtgact ttcgtaatct taggtggcac aggcggtgtc
     3421 aaatctcggg gtgtgccgga tgcaaaagct gcggggctgt attcggcaaa actgttacca
     3481 ccgtaaaggc ggtaataata tgtagtgccg ttaataatgt cgagcgaatc tttataaata
     3541 aaattattag ttaaagtgtc gttagtagaa ttaaaaaata agggtcttga tgtgataggc
     3601 tcaaagtttt tctgatctgt agatcgttct attttgtaat aggtgaaatt ttctacattg
     3661 tccttactcc actgaagagt caatgtattg tctccatcta aaacctgaaa tgctacaggc
     3721 tcttttatgg cctgtaaagt atttactaat tcaattactt cattgtattt actatcttta
     3781 aaccctgctg tactaatttc gtacacatac ttctgacctt tagaaacatt acgatctaca
     3841 tatcgaaatc ccagaatatt ggcagcatcc ttagaaaatt ctgagactaa catagccata
     3901 ccgagaatat tctggtctgc ttgatacagt ttatatccct gtaaatcacc cctgtctata
     3961 tttccatata gtaaaaaata tgcgacctga gaaattgaat ccatttcgcg taatttcggc
     4021 tcagaggatt ccataggata aggaaatacc ttagatatct ccttcaagtt gtccttagag
     4081 gtgcctcttt ttatggtaaa tccattttgc ttgacacttt cccaaaaaag ataatatggt
     4141 ggggacattc gcaagacaat agagtcctcg taagcttttg ccataccgat aattttatca
     4201 tcgttgttat gtacagggag tgtgtcaaaa ctcgattgat tctgttttat cttgtgtgat
     4261 ttcaagtgtg gttccaaatc gcttatgtga ccaaaagcaa ttgcctgatt taaaaccaat
     4321 ccctgaaaaa atactatgat taaaatcaaa cttatattct tcatctatct ttcaatttta
     4381 tacgattcat catcgccttg ttaaaatatg catacataaa aatggcttct gcctcattat
     4441 ttcaaaacct gattagaaat aagattctcg ttttgactta atttagttgt tactcatcgc
     4501 ctgttttgaa tattatatta tgaatctcag gttttccggg tatcgaactt atacctccac
     4561 tatccctact aaatagtagc ttatcatatt taactaaaag gttgtaagtt gtattgggtt
     4621 ttaagcccgg agcatttata gggcaaacag tcactaaccc attctctcca gttgtcggtt
     4681 tacccaattt tatcctatta gctgtacctc tctgatttat ttcgatacta cttaatgaat
     4741 aacgtacaga attaaactcg tcaagcctga tttccttatt catcgcagca ttatgtctga
     4801 attttatttc cgttgatttc gaactaatcg tttctccctc cttaggaaaa ataaattcat
     4861 tgtggattgt ttctttcggc ttatcgactg tagtaaactc tgatgattcg gattctactc
     4921 ttactatttt ttcattgcta accactcctg tttccgactt atcatattgc atccaattgt
     4981 atttaacttc gagaagataa gtcgtattta gcttcaattc ctgtgatatt ttataaatgc
     5041 tgaagaaaag accgttatta taacccgtcc cgtcttcata cctaatttta ttgaccggta
     5101 ctgcgactcc ggataacttc tcttttatag aaacgatcaa atcgcacttc atgaattttg
     5161 gcgtgttatt ttcagatagc actagccttt ctttctcgat atctgtttta tgcttaatct
     5221 ttatttggtt aaagttggta gtgactaaat tattattaat ggggtcgtca ataccaccgt
     5281 tcccaataat gtctgcatcg tcaaatttag tttttaatgc tacatcatta gttgttttat
     5341 ctaaatcttt aggtacaaat tcaacataaa ccggttccac aatagggtgc atatggcctc
     5401 cttttttatc cacataattc cataaaaaat ttcctttaag tatgtacgtt gtattaagat
     5461 ttaaattaga aaactccaat ggataatttt ctgtatcgga cgaataaggg gatacaaagc
     5521 ttatcgtctc attagtcaat ttatttataa cagtcaactt aggtgtgatt tccatggatg
     5581 acccatcacc caattctata tttctatcta ttggatggct aactttcaaa actgcaaaat
     5641 caggtccata ttttcctgtt ttatttaaat tgacctccaa tcctttaaaa gcggggcttc
     5701 cataaaactg tgaagttata aaagttgcat tcgctagttc ttctttaata gattgcttat
     5761 taaacatctg tcctgcctct gtcgtttcat aagaatacag ggtgtactta gctttgatta
     5821 gatacgcttt atttgcctcc aacttttgtg tcaaataata attattcata aaacctaaat
     5881 aggatttagt tgaactacct ttaagtaaag aaatcgtttt tccggaaata gaatcttcca
     5941 attttacctc aagatcacat tttatatatt tgaccttatt cgctagtttt attttaaaaa
     6001 cttcatccat atttatattg ctcgcaattt ttagactgtc ctgttgtaac caaacagtaa
     6061 ttattccttt atcttcttta tacgacgaaa gatttttaaa cccaccttca aaccgatcgt
     6121 tttgacttcc aaggactaat gaattgtgga tatctatatt ataagcgggg cttgtcgcaa
     6181 attcaccgaa tactgtttct ttactatgat ctattaatgg gtttcctgca cgttctatgt
     6241 atcgcgcctc catggaatag actgtattag gttgcaataa aaaatcaggt tcaaaataga
     6301 gaatgtcatt atttaaatta ttacttataa aattttgaac tattcttaca accttacccg
     6361 tcgccttgtt ccttatgatt aagtttgaaa gaccaagatg accttggtac ggagccatat
     6421 tgtgtctgat tttgaaagta ttaattttgt tgataaatcc gccattttca gggaaaacaa
     6481 aatcattatg agcttttatt tcgtaataag gactgggttc aaaggtcctg gtgaatatct
     6541 gatcatccat cttctttata ttgatttctt ctccagttgc cgtcttatta tacgttctaa
     6601 agtgatacgt cactttggca gtatagcgca tacctcccgc tttaagttgt tggttgagtt
     6661 taaatatact tacagattta ctgatgtctc cgttatatac cttctcattg attttatcca
     6721 atcgggctat ttcatctcct gaatatttat taattaatga tacgtcaata tcacatccta
     6781 aatactcttt tgaattatct tcggtttcca ataattgttc ttttgaaaaa tcgaattgat
     6841 gtaaaactgt tagactgtcc aatttttggt aaagaatatc accaccttct aaatcaactg
     6901 ggtcatcatt aaagaagata ccatcatgaa cctttattcc ccacaatggg ctggtcttaa
     6961 attcttttgt gaccacttct tctgctattt tagctgcata tccagttgcc tgataaaccc
     7021 cattttgcat agtaaagggt gctgatgtcc aagtgtattt agcttcaata atataggatt
     7081 tattgggctt catcaaacta tagggcaaca tacagacatc atatagttta tctttcatat
     7141 agagtcgatc cactaaagga ataatttttc cggaagactt ctctttcagt gtaacatcaa
     7201 ggctacattt taaggttttt ccatcattgc ctttttttct tacattaaag tttttttcta
     7261 ataccacatt gttttttact ttaacatgta cgaatacatt atccccattt tctttatttt
     7321 cgaaaaattc aaaatcgtgg tgggctttta tcccaaccaa tgggtctgct tgatagtcat
     7381 cacaaggttt ttctcctaaa tcaatttcca tattttgcgt tcccgcatac gatcctccca
     7441 acacattata tttgacggca aaatctccta gcaaataaga aggattagga aagccaaaat
     7501 ctaaaaccaa atccaccgat ccatcaaaaa tttcaaactc tttaacccaa gtgtcaatgg
     7561 atgctgtcgc ttttaatgac gcataagcat tcccggttaa ataataaccg ttttcaaatc
     7621 ctactttctc ctttttacca ttacatgtta cgcctttacc ccagttttga attgccagat
     7681 ttgctccaat ttcagctgta gcgtaccact tgaaaataag aaattcactg cccatgtcag
     7741 cacttaatct aaagccagca ttcgctgcaa accgatcgga tagtatagaa gaactctcaa
     7801 ggctattatc cgtaccttta taattaggca ataaataact aatcttgggc tgtccgtctg
     7861 ttttatttcc taattgtgca tataatcctg ccgaaacctt tccaaaaagt ggcgcttcaa
     7921 accctgcaga caaaaagtta agacctgaag aaggcccaga gcctaatctg cccgggatac
     7981 cccaactacc ggcatgtaca taattgaatt cgttggaaaa acagaatctg atactgttcc
     8041 aggagttaga attactgttt ttatccgatg gtgcagctat ctcgagaggt cctgcttttt
     8101 ttccaaatct gaaatctatg ccacctatta ctttaatgtt ttcttcaaaa ttcagattta
     8161 tatctccttt tactatcagg ggactttcag tctttctggt ggcaaaatct tcatacaagg
     8221 catatccatt gagacctaat ccgaaattct tgaatttaaa ttcatcatta atgatggcta
     8281 tttccagaga caaaataccc tccagcgaca ccctcgccgg atgagagatt tcagcagata
     8341 aatagccgcc catgcttcca cgacttacag tatattttag tccggatagt gacttaccgg
     8401 cttccattcc ttcagcctta cacaggtcat tgtttgctac aggtggtggg ctttgttttt
     8461 tgatctgatc caaacccgga atctccttgg ccatattata atagaatcca ccaccaatac
     8521 cggtcaaaga aaaggtggac tgccccaatg gcaacccagg attagcacta aatgacaggt
     8581 ctgcaaacca atatctaaaa tcctgagcag cctgagcggt agctccgtca agcttaacac
     8641 ctgtctggaa ttttgtggca aagttataat caagtatgct cacatcaatc atgcccaaaa
     8701 atccattgcc ccacgcactt tgaacggcat ttgggtcata gttccccttc gtagtttcat
     8761 cccggaataa attaataccg cctttaaatt tcaccggccc aaactctcct cctaaaccaa
     8821 ggcaggtaag ggctacatct ttatatcctt tgccatcgaa ggacacttct atatcaccac
     8881 tcgcagagaa ggcacctgtt tttgtagctt tagcttcagc caggtctttt ttaaattttt
     8941 ctttgtccat ttccagcctt tcacctgctc ccgcattttt atatttttca gttctttcga
     9001 caacggcttt tgtttgcttg tccagttcat tagatgcggt cttcagtttg gcactttgtt
     9061 ctttgaacgg cttatatttt tcctttactg atgtaagagc tgttttatat tttgtatttg
     9121 ccgccgtaat ttttgtatta agggaagcaa tggtactgtt tcttgaatcg atctctgact
     9181 tatttgaagc actgttatag ctcacattat taccattaat tttaatgttt ttatcctttt
     9241 taaaagtttt gagctctgtt tctaatcttt gtttttctgc aagcagctta cttctttcat
     9301 ctgcgatggt tttgatgtta ttctgtgcct ctttgtagtc tttctccagg ggttttaaac
     9361 tgttttcttc ttctgccttt tttctttctg ccgcttcttt gggattgaat ttgtcagaga
     9421 cctcttgttg tgaagcggtc agattacttt tatccctcat gatgctcaga tccagtccga
     9481 tgaccatctt ccattcagag cctgtgcaga aaaaggatgg ttcatgaata ctgatatcaa
     9541 atcctaccat tttactaaag ctactaatgt cggcacccag tcctttggca aatttgctct
     9601 ctttgatttt tccggataaa ttttgacctg tgctcgaatt agcggccttt gatatggctt
     9661 ctttttcaac ctcagtaaaa tacatgatac tccatgttcc gaaatcaatg gattcaatac
     9721 ctgcaatatc attggacgag cacatatcag atccgttatt tttctttatt atgttatgat
     9781 tcacattaaa attctgaaat tttaaaaacg gaatcttggc atccaatttt tcgataaaat
     9841 aaatggctct gccattcaga cttgctttgg cagtcgttat ttttcgatcc ttatcatagc
     9901 caaagtcaac aaaggattcc ttatcgaggt tcatacccat accgtgtata taatttagct
     9961 caaataagct ctcattcacg ccatcaacaa atcctgaaat tgccggtatt tgttcttttg
    10021 cgtcgtattc aatactcagt gagtaaggaa cccatgcatc cgtaaatgtt tcctcccatt
    10081 ttgctttatt gtcagcaggt gctgatctga agaggggaat caaggtagct cccttcagag
    10141 ttaatttgtc agcatccaat tgattatatt ctatattaaa ccggatggta tccaggtggt
    10201 actgccatcc ccctaagcgc gccagatttt ctttttttac tttatggaag ccctgatatt
    10261 ttgaaaagaa agctttgcta tcaatctcat aaaatgaata ggatactgta tctttaaaaa
    10321 attcaggatt gcttttggcg tcaactctat tttgttcaag aataggaatt gaaaatccta
    10381 tttgattgaa gataattccc ctgaagtagc tattatataa ccgttctctc gtaagatcat
    10441 caatttctcc ctgtggccga tccaccgcag tagtactgct ataatccaaa tatgcctcca
    10501 atgattttcc gccattgaat acaatatgac gatcaccatc cgtcgaaaag ctccattctt
    10561 gccatccctt ggcactttta taagtagtct ttaaaggcgc tttaaattca gtcaaaagcg
    10621 caccgtttcc atcaatacta ttttctatca acctaaaacc aaatacagca ggtcccgtaa
    10681 ctgcaccggt ttcatcatac cctattaatg tttcgcttgg catggtataa tgaccctgaa
    10741 ggttgaaatt atggatgcct gcggtacagt ccaatcccac atagctaccg gtttcagcag
    10801 tcttgtctcc attgcctgag ctaaggtaag tcacgttttc atccaattta gcatctttag
    10861 tcagatgaag caaaatatcc tttaatgata ttttgtcagg ccctatggct atatcttcat
    10921 tgctttcaaa atacacgaag acctcttttt cctcgaccat aagtgcacca actaagccta
    10981 tcttaacggt tgcattgctt aaattgtcac taccagcggt tttaaattcg gtgataaaaa
    11041 gcttgtagtt ttcaggtaag gtataatctc ccttgaaccc aaattttgac cacaactctt
    11101 cggtaatgat caaagggaga tgaagatctc cggtggattc attggccagg ctgttgaaca
    11161 cctctttcgt ccactccgtt ttactattta cagcgactcc atttctatat ttacctgtgt
    11221 atgctccgcc tatattttcg atgctcaggt tcctatccaa gaatggtata ttgatcgcac
    11281 tattattatc tatggtaggg aaccaagcga tggtgttcat ttttattttc tcaatggcca
    11341 gctcttttcc aatgtaaaat agcttttccg gaaatacgga ttgtgtacca ctttgggtga
    11401 atccggctgc acttagtatg tagtgttttt cataaaaatc atcaatgatt ctatcccatt
    11461 catccttgct tggaattaca cttgctacac cacagttgca tttcaggtcg atattattta
    11521 cctcaatccc ttcaaacctg aaagtgcctt tatctgctgc tgcccaaatg gtcagctcta
    11581 ctttgctgga tcccttcacc acaatggttt tcaggcccaa gcgttctctt ttttcttccg
    11641 tgacatattt caatgaggca ctactgagtt tatgcgatgt tccgccaatc tgaagactta
    11701 catttttaag atcaaatcct tcaattttaa accttttttc tcgttcattc atcgggtcat
    11761 tggacagtcc atcgagccat aatccggata actcaaatgc gaagttatcg atcttaagtg
    11821 cgtttttcaa tgcatcgagt gtttcacctt tattagtatt caagatggca gctctgaatt
    11881 ctttatcagt aaaattgaca tttaagtcac ctgtaaaatc gcactttgga gtaagttgcc
    11941 tttcttcacc caactgaacc aatcgagcat ctaccgaaga aggtttttga gtagtaaagg
    12001 agccatgcca gatactcatg cctagttttg atgaaggaat aattgcatcc aactgtatct
    12061 gtttttgcct tgtttctgct actactgccc tataggggaa gtgatcatca aagaaaggtg
    12121 tcttaatatt gccactcaac ctgaattttc cgatctgatg tgcatccatc gttaccttca
    12181 tggtatcgat actatagttc catggaccca tctttccttt gtccaatcct aatacattgg
    12241 tttttgaaaa atgaccgtag gccaatttgt tttcatctat ataaatttca ccgttatcca
    12301 atgtcagagg cgaattggga tcaacgatat tgtatttttc agggatagta agctgaatat
    12361 ctttgagtcg caaccctttc cagttgattg attgctttgc agtataatca ttgacatctt
    12421 tattaaatcc attctcggat gaacttagat ccagttttcc actttttgcc tgaaatgtaa
    12481 agccgggcag attatttaat ttaaacctcg gtatattttc tacttcagtt atcttattgt
    12541 catagttgcc tgtttctttc gtaggttcga aagtgacagg agcaccatta tctgtacgtg
    12601 tcataatatc gggcgagata ctcagttgaa cctgatcatt cagcgattca aatcccttac
    12661 agttacacct cattttactc ttgtcagata ccacatttcc tgactcgatg gactccagta
    12721 attttactga agatcgttct attccatccg catgaatggg tatgagatat gcattatttt
    12781 tcactccaaa aggagttgta ggaatcaatg tcgctacata aataccacca tcgtctgtct
    12841 gagaaagcaa attaatgaaa gcattggtag gagttaatac cattccaatt actgcaacag
    12901 gaggttgttg cttttcaaaa tgcagggata ctgggagtga attaaagaca gtagaaccct
    12961 ccgccaattc tgcaacatcg ctcttaggct tttgcaacaa ggtattaagc ttgtcaatat
    13021 atttggaagt gatagctttt tctatagatt ccttgctgag attgtacaga ttaaattgca
    13081 gggcaggatc tattagagca gagatacttt cagcttcata gacccggcct tctttattta
    13141 ctttaatatt tttaaactct acttccaata ccgaacgcaa catcggatat tcgaccaatc
    13201 ccttgccgga ataactttgt gtctgactgc tataagcttc agtgatcttc atgtcgaaat
    13261 aaccaacctt tacattctgg ttgggcgagg ctatcactgg cacattgtct gcctttgaaa
    13321 ccgcgccata ggatgtctca taaacagagc acccacgagg tgaagctttg tcaaatgggt
    13381 ttacatcgcc tgccggtacc tctccttcat ataagataaa aatcgacact tcgcctttgc
    13441 catcattttg aaataatttg ctggtcggat acatgacaga agtggccgtc actcgccatg
    13501 catagtattt attgggttcc agtgtgggtt caccttgggt atatatcaat gaagtggcca
    13561 ttgtagtagt tgtatatact ttcagtgcac tagcaaagac atcattggca ttcatcacgc
    13621 ctacaggcag ctctacgagt tcgaaagtat attcgaccgg gccaggattg tttgggctgc
    13681 cgatatgcat cggttgccat gaaaatatgt aattctgcac ttccggcatc ttgatttttt
    13741 cattgaaggt cggctttgag atttgaggtg gctgacacaa tctaaaattg cctgaaatac
    13801 aaaatttatt ggacacaggt acattgcgtt caacgccata caattgaagg cagacttggt
    13861 taaatccttc aggcatctca acggatcctt tactaccggg ctgggcacct gtcagtgcta
    13921 cattagacaa atattcacct agggcagaac cattgagaat gattggatta aactggctta
    13981 gcaaaatcgt cttgcccgca taattagggt ctgtctgata gataacagta ccattctgtt
    14041 caactgtaat aaccggcttg accaataaat tcgcttccac ggggtctttt aatattgctg
    14101 taaatgataa atcttcggcg cgttcaaggc tgtaaacctt gaggtcaagt gaataaggtg
    14161 ggagcatact gccagatacc tgtaccggcc aaacttctgt ctgagcctgc aacatgaaga
    14221 ttgatacagt tgaaaatatt attgaaaaga ggtattgtct gatattcatt atacaatatt
    14281 ttatttggtc aaatgcctat ttttttattt tatcccgttt tactctgctt tattattttt
    14341 ttccttttct tgcttacttt tctttttggg aacatagcta aactggtatt gcatggtcaa
    14401 atttcctata ctttcagtaa aggctggtcc tgtgccagta gtagtattga gaagccccca
    14461 ctgtagatta atacccatac ctttaaacaa ttgataagct accataaatt gtcctatggc
    14521 gttttttgat ttttgtccaa gtgcgttttt attgttgtag aagttgccat ccactccaat
    14581 agttagctta ttttcaaaaa aggccttcct cacacccaag ccgtaaccga ccctatctgt
    14641 ctgcagacca gagatttcat tttgggtata gttgcccctg agtgaaaaat tccagtcgga
    14701 tggcaagcgc agcatatagg aaaggctacc tagcaagacc ttcgaagtta gggtgcgact
    14761 tggcttctct atgtcgtcgc ttacatcctg aatattaccg gtagcactga tagcatgtga
    14821 tatctcattt gtggttggta gattgtattg gatattgagc cccatgtcgc gggtgacgat
    14881 gactgcattg agcgaatcca gattctgatt gagcagataa ttgactcttg ctgtattatt
    14941 ggaataatta aagctggcag atacctttgg aatggcatag gtcaactggg catcgtatat
    15001 caatctttta ttggttgtag ggagactttt atctaaattg tttgactgaa tccctgcctt
    15061 gagtgatgca ttgacctttc cgtccaatag tccaaattgg gtaaaccccg aaatatccat
    15121 tatatctcta ttgaagaagt aagtgcccaa tgtcttgtag tttgagtcca cccgattgac
    15181 tataacaccg gcattgaagg tcttagtttc gagtacgata gaaccttcga tcgcatgggc
    15241 aacatacgtt gtatttttct tctttaacaa gaaattggta atgcctgtgt caggagagtc
    15301 tgcatctagc gcatttggag atattgtact gttgccataa tccaccttga tacggactag
    15361 atcgaagaat agtttttgaa gggtaacgcc gagggctagg ttagcctccg gtgtcacttg
    15421 ttcaggataa ttagcaggga tggatataga attttcatca tctcgggcag taaatagtgt
    15481 cagatcagcc gacgccttgt catcaccata tcctaactta gcggcccaac ctattcgttg
    15541 gtacaccggc agattaggtg tagttaacga caagtcaatg ggttcagcct tagcaagcct
    15601 gccatacatg cctccaatgc gtattttacc tggatttagt tctagaccgg caccaaaaaa
    15661 gttcaggttg ctcatcgtgt atttagaaaa attcatagca cggtgcccca ggtgaatttt
    15721 ggcccatttg taatatggac tcataccgat gcgggcaaat gaattggctt tgtccctcag
    15781 attttgcttg agagactctt tgagatccga aaaattgggg taggagcttg ttgtattctt
    15841 tgcagtcatg actactgata atggaagctc tatttgatat atgcgtacat tgaggtgccc
    15901 attgatggta tagaaaaatg gatcttgcct caatggacct cctacagcat tatacgaacg
    15961 caatccaata ccaataccac cacccagctg aaacggctcg ccgaagtcag caaaaccttt
    16021 aaagaagcct ttcagactgc ttttgaacaa accgatttca ttgtattgct ccaaattttc
    16081 gaaagaattg agctgtccat acatcttatt atgtgttaag caaaacaacg taataaaaac
    16141 cagaaaaaga ttgcggatac tcataactac tgaatattta gtgatgtaga attatttggc
    16201 aattattgat taccaaatat aatgtatgct attattgcat ttgtaagtat gggatagaaa
    16261 ggcgtattta gggatagaaa tataacccaa aatgcgtaat agaaatgcaa cgaaaatgaa
    16321 tagtgcaaaa tatctataaa ataagtgata aaatcattgt gaacaatggt ttttgagctt
    16381 ttgcaatgca tttgaagtaa taattgttgg tagtaaatag tctaaagcag attctggaat
    16441 aattagacaa caccagatca atcaggcaat acagaggcct tcaaacatca cgttgtgtct
    16501 acacacatct gttggtttca tcagggcatg cccct
//
LOCUS       DAOQQN010000012        16472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1095_length_16472_cov_5.053664, whole genome shotgun sequence.
ACCESSION   DAOQQN010000012 DAOQQN010000000
VERSION     DAOQQN010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 16472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16472
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1095_length_16472_cov_5.053664"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..1162)
                     /locus_tag="PLG24_00935"
     CDS             complement(230..1162)
                     /locus_tag="PLG24_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMZ23473.1"
                     /translation="MSIPMIILLVFAALFVFSGLVTVRQGTVAVITMFGKYQRILTPG
                     LNFKIPFLEQVYKTISIQNRSIELEFHATTSDQANVHFTSMLLYAVLNNSEETVKAVA
                     FKFIDERSFMQTLSRVVEGNIRAFVATKKQSEVLSLRREIVEVVKGNVDAMLEEWGYH
                     LIDLQINDISFDEEVMRSMAKVVASNNLKAAAENEGQALLITKTKSAEAEGNAIKIAA
                     EAEREASRMRGQAVASFREEVARGMGQASREMHEANLDTSVILFTMWTESIKQIAENG
                     KGNVIFLDGSVEGMEKSIKNLMAINQLDLMEKRK"
     gene            complement(1254..2138)
                     /locus_tag="PLG24_00940"
     CDS             complement(1254..2138)
                     /locus_tag="PLG24_00940"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HMZ23474.1"
                     /translation="MHFEPSEMATKDLHQLLLGIVAPRPIAFVSTLDEHGIPNLAPFS
                     FFNCFSSNPPIVIFSANRRVSDNTTKDTLANVKASGECVINAVSYHVVRQVTLCSVDY
                     PSEVNEFEKAGLTPIPSDLVKPPRVKECLAHLECKVKDILPLGEGGGAGNLVICEVVR
                     IHADSSIMDENHRIDPNKIDLMARMGRAFYSRASGDAVHTIVQPYEASPIGFERLPEF
                     FKSSHILTANDLASLASIEDVPGDEDLAAFTNREEIRSLLAQQEASALLQQYAHRVLA
                     EDRKKEGFMAMHLAANRE"
     gene            complement(2151..2519)
                     /locus_tag="PLG24_00945"
     CDS             complement(2151..2519)
                     /locus_tag="PLG24_00945"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:NF014233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HMZ23475.1"
                     /translation="MILISGFEKLSLHAPVGLYPEEKVLGNDFLMDIEVAMDVPDGFL
                     IEKLENTVDYSQLEKIARENFTKGSDLMETGAQMVIEAIQSEFFGILGIRIKVRKVHP
                     ITLPSVDNTFVELKTGYFAK"
     gene            2613..3386
                     /locus_tag="PLG24_00950"
     CDS             2613..3386
                     /locus_tag="PLG24_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HMZ23476.1"
                     /translation="MPVLRSIYNTLLSLLAYFRIFCCVLFLVIAVPFLHLETMIFPNK
                     EKIGFRYRKWFTSLYLFILNIKVKKSGEIAAHNALIVCNHRMMIDPVIVLDYCPGYIV
                     SKAEVASYPILGHGARNAGVIFVRRDQKESRNAIKEKIGELLSEGKNVVLFPEGTVNT
                     DRLTARFSVGSFEEAAKYGRPVIPVALDYKDTSVYWGANISLIQHFLQVFRKPRLETR
                     LVIGSPIKGNDGFELMESAKNFIDNQLEVIRRDWETSQI"
     gene            3425..4897
                     /locus_tag="PLG24_00955"
     CDS             3425..4897
                     /locus_tag="PLG24_00955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMZ23477.1"
                     /translation="MMHDRKYTALIVVALVGILLFLHRGHFNLELRGTHVWRQSITAL
                     HIRNFVRYDNNILNPRVASFNGGRDNISRYEFPLMQWTIAQVERVTGESVAVVKFYQF
                     LIAISFLIGFMFLLKRAGFDRRFCWMGIWAVAFSPVIYYYSVSPIPDMLALSFATWAL
                     YHAIGFFRSEQNKYWPIFNIGLLISLATLVKLPYILFMGPAVYLIIAKYRMRWKSYFV
                     PFFILAACMIPAFLWYAWVIPQWTVGITKGMFDNQISMDHALQIISYHLKVMFPYRLL
                     NVVFVPFFLLSVIPMFRSIRRDAVLQSMLLGFLAYLAYFLFELNMIDVVHDYYMMPFV
                     PFLFWGVMTGIRFCWQLRYMAAKVIVGIALIVAPFTCLENTQKDWEITHFHDIYEKQA
                     EFIGAAPPNEKVIMLKDFSGFILPYLIDHHGHIFNFDTLPRSWVKNMISEYDVHYLYS
                     NSRTSDSINRDFIRDTVFKHGEFKVFRLKDKTAVDTIPAE"
     gene            5192..8134
                     /gene="dnaE"
                     /locus_tag="PLG24_00960"
     CDS             5192..8134
                     /gene="dnaE"
                     /locus_tag="PLG24_00960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HMZ23478.1"
                     /translation="MFLNTHSWFSLRYGTLSPERLADTARSLGIRAMALTDINNTSCA
                     WQYIEACRRNDVRPILGIEFRRDDGRMLYVGIARNNTGWANLCRMLSEVSLDGKSLPA
                     CAPELDSVYFIYRNQPKDIRTFRDDEFYGIRPDEARALYSSPLRKHMDRLVAFTPITF
                     ADEDGYKIHKLLRCIDRNILLGQLRQQDCAGSDEVFMTEAALRDAFALFPDIIRNTEM
                     LTDSCSIDMTASKINNRQCFTDSKDDDYRLLAKLAVNGCIRRYGRHNEQANKRVDSEL
                     RVIRELDYSAYFLITWDIVRYGRSVGYFHVGRGSGANSIVAFCLGITDVDPLELDLYF
                     ERFINPHRTSPPDFDIDFSWDERDDVTDYIFKRYGREHTALLATYNTFKGKSIIRELG
                     KVVGLPKSDIDVIVDHPLDTDRHHPFGKHLFRYGKMMEGMPNYLSIHAGGILISERPL
                     YEHTALKLMPKGFPVTHFDMYGAEDLHFHKYDILSQRGLGHIKDAIDIIARNRGRIVD
                     IHDIYRIKHDEAVRAQLRSGHCTGCFYIESPAMRGLISKLKCDNYVHLVAASSIIRPG
                     VAKSGMMREYIQRAHHPDKVQYLHAVFEQHLAETFGVMVYQEDVMKIVHHFAGLGLDE
                     SDVLRRIMTGKKKNSDTFRDLQARYFDNCRARGYSDALTNEVWRQIESFSGYSFCKAH
                     SASFAVESFQSLYLKTYYPMEFMVAVINNFGGFYSTELYVHEARVSGATIHPPCVNHS
                     DRLTNIHGTDIHIGLGLIRDLEYTTIRNILKARAEDGPFRGLEDFVSRVAIDATQLTL
                     LIRIGAFRFTGLNKYELMWEKNTVYNARHRETVGMPVLFEPEHEQFSLPVMTEVDYEQ
                     AFDEIELLGFPLTSPFELVEDELPARIPAQDMMRHAGRRVTMVGYYVTRKPVRTVSKT
                     LMYFYTWVDEAGDFFDTVHFPPAVNRSPFKGSGCYIIRGKVVPDFGFPSLEVDSMEKM
                     PWIKDGRY"
     gene            8344..8817
                     /locus_tag="PLG24_00965"
     CDS             8344..8817
                     /locus_tag="PLG24_00965"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HMZ23479.1"
                     /translation="MIRTLLFFFITCISVAVFAQPPAGDANVGDYYGKKIKAKGAMDV
                     AVLPAKLRPDAPIKVKVKGTVLDACAEKGCWMVMKIDDTNTVRVRMQDYAFFVPIAII
                     GKTVVAEGEAEMSVLSVKEQKHFAEDAKKSQEEIDAITMPKKEISLMASGILVVE"
     gene            9018..9425
                     /locus_tag="PLG24_00970"
     CDS             9018..9425
                     /locus_tag="PLG24_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23480.1"
                     /translation="MKHAFKAFLLLMIFFIVGSVNAQNRSGDDGQASMRAAKIKSAKK
                     KDQSTQQKSNPAHASLFDNLDTNKDGKVSREEANKSNLVIIKEKFDNLDTNKDGFLSK
                     AEVQNARYNVNKGQQRTTSKVKSSTTNTAAKKQ"
     gene            9646..11304
                     /gene="recN"
                     /locus_tag="PLG24_00975"
     CDS             9646..11304
                     /gene="recN"
                     /locus_tag="PLG24_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HMZ23481.1"
                     /translation="MLQRIRVRNYAIIDEVSIEFDPHFNIITGETGAGKSILLGALGL
                     ILGNRADSKTFFNTEEKCIVEAEFSIGSYSLHPWFEANDLDYEDLTLIRREIQPAGKN
                     RTFINDTPVTLQQLQEFTQQLIDIHQQFSLYDIQKPAYQLMLFDAYSGNKVLMGQYSE
                     LYRKYIALGRTLKELQEERQKSLREADYLAFQLEELNSVDFVNMDQDALEEELNMIDH
                     AGTIKSTAGAAYVALYDDDNNIISAIEDILNSFGPITAVSPRIKEVVDRLQSSRIELR
                     ELAEDINRLAESTEIDEERAAEIRESLDQLYKLQSKHQVGDIAGLVALKEEIDGKLNN
                     YSKVDDDIVRIEQEMSHHEEVLNRMAESLSESRKSHIPDFIDTLKGLLSDLGMPHATF
                     VIEMEPSTALTPSGKDAIRFLFAANKGGQPAAIKDVASGGEISRLTLSIKSMVAAAIP
                     LPSLIFDEIDTGLGGEIALKMAEIMREMAREHQLIAITHSPQLASKADKHFFVYKEIE
                     DNNTFTRIKELSLDERIENIAIMLSTKPPTKAAVENARELLERK"
     gene            11334..12140
                     /locus_tag="PLG24_00980"
     CDS             11334..12140
                     /locus_tag="PLG24_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ23482.1"
                     /translation="MANGLLKGKKGIIFGALDEKSLAWKVAERAYEEGASLVLTNAPV
                     ALRFGQINALAEKTGAPVIPADATSSEELAKLIDDSINHFGGKVDFVLHSIGMSLNMR
                     KEKHYTDLNYDFMHKTLDISAISFHRILHELYAKNAVADWGSIVALTYIAAQRIFPDY
                     NEMADAKALLESISRNFGYHFGTRNKVRVNTISQSPTMTTAGGGVKGFTDFFSYADTM
                     SPLGNAGGDAFADYCITLFSDLTRYVTMQNLFHDGGFSSMGINPKLLGVE"
     gene            12307..15282
                     /locus_tag="PLG24_00985"
     CDS             12307..15282
                     /locus_tag="PLG24_00985"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HMZ23483.1"
                     /translation="MSKFLIAFIFFLFTGASLTAQDFFSITTESKADFIDKNKEYIKP
                     ERKVFFNYDYNSLRSILRTAPQESYFDRGLPGLKLHLPNPEGGLTEFDIYESPILSEP
                     EYSQFPNIRTYIAVNQANKIEHGRIDITEHGFHGLIFTDGDVYLIEPASLGNRQSCIT
                     YYKKDNKIRPDFECMTEGSGFNDVDLETRTPLSCNSSMTLVSYRTAVACTWEYSNFHG
                     GTVALTLSAITTTVNRVSAVYEKDMGARLILIPQNSSILYTTEGGYTASTDPYTNDDG
                     GLMLGENQTVLRSVIGAPNYDIGHVVSTGGGGVAWLAVVCFNTPGTNNSNNANLKALG
                     VTGASTPVGDYFDIDYVAHEIGHQFGANHAFNGNTGSCSGNRNGSTAYEPGSGSTIMS
                     YAGICGAQNIQPHSDPYFHWVSMGEALTHINTTTSCRTAVSGTNNTTPQITSFTTGKT
                     IPANTPFYLDASATDADGDAITYCWEENDLGTAGNINASSTTAPIFRSFNPVSSGRRY
                     FPRLHDVLNNTTTYGEVLPSVARTLKFTITARDNHATGGGICRQSSTITVVASSGFKV
                     TAPNVATTWSSPGTGTITWDVAGTASSPISCANVDVQVSFDNGLSWTSIGVGVPNNGS
                     FIWTIPSGINSSDVRFRVRCSDNIFYAVNTVPITIGNPVNTCYTYTNNSTLTIPLTPS
                     TVTSSIIISNRAADEIVKDINITNLNVTHSNLRDLRFKLKSSNGASNILMDSKCSGYA
                     NINIGFDDEAVSGTLPCPPTNGGAYKPQEFLNVHDGMPANGQWDLEIDDWYSGNGGSL
                     NSWSVQICVEKKTPLPLHLLSFAAEAVKIGVYIGWTTSNEREVEVFTVERMAHDGSFR
                     SIGHIPAKNIDAISNYGLVDEYPDKGINYYRLKMIGKNGKVSYSEIKSVRFEGRSSVT
                     IFPNPTNGILSIKRIGNEGEIHSINILDLSGREVMSLPMRLHLYELKDIDVSSLIPGM
                     YLVQVNMNTGVETIPFIKS"
     gene            15901..>16472
                     /locus_tag="PLG24_00990"
     CDS             15901..>16472
                     /locus_tag="PLG24_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HMZ23484.1"
                     /translation="MIISVLRESKDGERRVALTPDVIKLLIKKGFQVKVESGAGSNSF
                     FSDDEYVRAGAVIASTRQEACSQDIILKVNAPSTEEVSMMKTGACCISLLYGYTQPVL
                     LDAFNARSITSFGMDAIPRISRAQKMDALSSQANLAGYKSVILGCDALSKIFPLLMTA
                     AGTITPSKVLIFGAGVAGLQAVATARRLGA"
BASE COUNT         4463 a         3546 c         3791 g         4672 t
ORIGIN      
        1 gtcaaagcag atgatgctgt atatgacaag ttgcctaatt gttgccacta cgatagattg
       61 aagtaaacta tttgcttcag aaatcaaaag gaggaccgac ttgcttcgat cctccttttt
      121 tttgttccga catcctcatt gcatgaccgg aatttaacac aatttatttt ttcacaggaa
      181 tggagggaat cagagttccg agtctccatg gtctcacgat tcctgtttgt tattttctct
      241 tttccatcaa atcaagctga ttgatagcca tcaggttttt aatggacttt tccatacctt
      301 ccacactgcc atctaagaag atgacattgc ccttgccatt ctcggcgatc tgtttgatgg
      361 attctgtcca catggtaaag agtattaccg atgtatccag attggcttca tgcatctcgc
      421 ggctggcttg acccatacct cgggccactt cctcccggaa tgaagccact gcctgaccac
      481 gcatcctgga agcttccctt tcggcttcag cagcaatttt gatggcatta ccttcagctt
      541 ctgctgactt ggtcttggta atcagtaacg cttgcccttc attttctgcg gcagccttga
      601 ggttgttgga ggcaacgact ttagccatgg aacgcatcac ctcctcatca aaggaaatgt
      661 cattgatctg gaggtcgatc aggtgataac cccattcttc cagcatggca tcgacattgc
      721 ccttcaccac ttctacaatt tcacgacgca gggaaagcac ctcactttgc tttttcgtcg
      781 ccacaaaagc cctgatattc ccttctacta ctcttgataa agtctgcata aaactcctct
      841 catcgataaa tttaaaagcc accgccttta cagtttcttc actgttgttg agtactgcat
      901 acaatagcat ggaagtaaag tgcacattgg cctgatcaga agtcgtagcg tggaattcca
      961 gctctatgga tctgttctgg atagaaatgg tcttatacac ctgttccaaa aagggtatct
     1021 taaaattcaa tccgggcgta agaatacgct gatatttacc aaacatagtg ataacagcta
     1081 ctgtgccctg acgaactgtc accaaaccag agaacacaaa gagtgcagcg aaaactaaaa
     1141 ggataatcat tggaatactc atatctaaat ttttttatta attattataa attgctaaca
     1201 aagaatagta atatctaagt tcatcatatc agccaatttc gataaatctt atattattcc
     1261 cgatttgcag ccagatgcat ggccataaag ccttcttttt tacgatcttc tgccaggact
     1321 ctgtgggcat attgctgtaa taaagctgat gcttcttgct gggccaaaag ggatcgtatt
     1381 tcttcccggt tagtgaaggc tgccaaatcc tcatcgcccg ggacatcttc tatacttgca
     1441 agggatgcaa gatcattggc agtcaggata tgactggatt tgaaaaattc cggcaatctt
     1501 tcgaatccaa tcggagaagc ttcataaggc tggacaatag tgtggactgc atctccggat
     1561 gctctggagt aaaaggcacg gcccatcctc gccatcaggt ctatcttgtt cggatctatt
     1621 ctatgatttt cgtccataat cgatgagtcg gcgtgaatcc tgaccacttc acaaatcacc
     1681 agattgcctg caccacctcc ttctcccaga ggtagaatgt ccttgacctt acattccaaa
     1741 tgtgccaagc attccttgac tctcggaggt ttgaccagat cagatggaat gggtgtcaat
     1801 cctgcctttt caaattcatt gacttctgag ggatagtcaa cactacagag tgttacctgc
     1861 ctcactacat gataggaaac tgcattgatt acacattcac cactcgcctt tacattggcc
     1921 agtgtatctt tggttgtatt gtccgatacc cggcgattgg cactgaaaat gacaatcggt
     1981 ggattgctgc tgaaacaatt gaaaaaactg aaaggggcca gattggggat tccatgctcg
     2041 tctaatgtgg atacaaaggc aattggcctg ggggctacaa tgcccagcaa aagctggtgc
     2101 aaatctttgg ttgccatttc tgacggttcg aaatgcatat ctatggtttt ttattttgcg
     2161 aaataaccgg tttttaactc aacaaaggtg ttgtccacac tcgggagggt tatgggatgc
     2221 acttttctta ccttgatccg gatgcccaat attccaaaga attcagactg aatggcctca
     2281 atgaccatct gagccccggt ctccatcagg tcgcttcctt tcgtgaaatt ctcccgtgct
     2341 atcttttcta attgactgta atcgaccgtg ttttctagtt tttcaatcag aaatccatcc
     2401 ggaacgtcca tagccacctc aatatccatc aaaaaatcat tgcctaatac cttctcctca
     2461 ggatataggc cgacaggagc gtgaagggat aatttttcaa agccggaaat aaggatcatc
     2521 ggttgatttt agcggaaaat tattaattta tctgaaaatt gattcattca ttgataaaat
     2581 aaattataat tgcaggctaa aattcgttgc atttgcccgt actcagatca atatataata
     2641 cattgttgtc gctattggcg tattttcgga ttttttgctg tgttttgttt ttggtcattg
     2701 cagttccctt tctgcatctc gaaacaatga tctttcccaa caaggaaaaa atcggtttca
     2761 ggtacagaaa atggtttacc tccctatatc tttttattct gaatatcaag gtcaaaaagt
     2821 ccggtgaaat tgcagcgcac aacgcgctca ttgtatgtaa ccacaggatg atgatcgatc
     2881 cggtgatcgt cctggattat tgccccggtt acatcgtaag caaggcggaa gttgcttcct
     2941 atcccatcct gggacatggt gccaggaatg ccggtgtcat ctttgtccgg cgtgaccaaa
     3001 aggaatccag aaatgccatc aaggagaaaa ttggtgaatt gctttcagaa ggtaagaatg
     3061 tcgtcctttt tccggaaggc acagtaaata cggatagact gacagcaaga ttctctgtag
     3121 ggtcgtttga agaagctgca aaatatggca ggccggttat tccggtcgct ttggattata
     3181 aagatacctc cgtgtattgg ggagccaaca tctctctgat tcaacacttt ttgcaggtct
     3241 tcagaaaacc tcgtcttgag acccgtcttg tcatcggatc tccaattaag ggtaatgatg
     3301 gctttgaatt gatggaaagt gcaaagaatt ttatcgacaa tcagcttgaa gttattcgcc
     3361 gggactggga gacaagccag atttaactta ggtataatcc gatgggaaag attattttta
     3421 tagtatgatg catgacagga agtatacggc actcatagtt gtggctttgg taggaatcct
     3481 gcttttcctg cacagaggtc attttaacct tgaactaaga ggtacacatg tctggcgtca
     3541 atccattacg gcccttcata tccgtaattt cgtcagatat gacaacaata tcctgaatcc
     3601 cagagttgcc tcgttcaacg gtgggcgcga caacatcagc cgttatgaat ttccattgat
     3661 gcaatggacc atcgctcagg tcgaaagagt gacaggtgag tctgttgctg ttgtcaaatt
     3721 ctaccagttt ctgatagcga tatccttcct gatcggattc atgtttttgt tgaaaagggc
     3781 aggatttgac cgtaggttct gttggatggg aatctgggca gtggctttct ctccggtcat
     3841 ttattattat tccgtatcac ctatcccgga tatgctggcc ctgtcttttg caacatgggc
     3901 attataccat gcaattggtt ttttcaggtc agaacaaaat aagtattggc ccatattcaa
     3961 tatcggactg cttatttccc ttgccacgct ggttaagctg ccttacattc tctttatggg
     4021 gcccgctgta tacctgatta ttgccaaata ccggatgcgt tggaaaagct attttgtccc
     4081 cttctttatt ctggcagcat gcatgattcc ggcgtttttg tggtatgcct gggtcatccc
     4141 tcaatggaca gtagggatca ccaaaggtat gtttgataat cagatatcga tggatcatgc
     4201 cttgcaaatc atttcttatc atctgaaggt gatgtttcca tacagattgc tcaatgttgt
     4261 ctttgttccc tttttcctgc tatccgtaat tccgatgttc cggtctattc ggagggatgc
     4321 ggtgctccaa agcatgttac tgggcttcct ggcctatctg gcttattttt tgtttgagct
     4381 caacatgatc gatgttgtcc atgattatta tatgatgcca tttgttccat tccttttttg
     4441 gggtgtcatg acaggtatta gattttgctg gcagctaaga tatatggcag caaaggttat
     4501 tgttgggata gcacttattg ttgccccttt tacctgtctt gagaatacgc aaaaagactg
     4561 ggagatcacc cattttcatg atatttatga gaaacaggcc gaattcatag gtgcagcccc
     4621 gccgaatgag aaagtcatta tgctgaagga tttttcaggc tttatcctcc cttacctgat
     4681 cgatcaccac ggtcatatct tcaattttga tacccttccc cgaagttggg tcaaaaatat
     4741 gatcagtgaa tacgatgtgc attatttgta ctccaactcc cgaacgtccg attcaatcaa
     4801 cagggatttc atccgggata cggtatttaa acatggtgag tttaaagtgt tcaggctgaa
     4861 agataagacc gcagtggaca cgatacctgc tgaatagctt tttatggctt atatggcctt
     4921 tggcaggagt aaataatggt tgcagatgcg ttttcggcga tagccccaat ctatatccga
     4981 tcattcccta cctattgatg actttccgta ttttatgatt agggcaatcc tgacagcact
     5041 gatacctcct tcattctatc caaagcggtt tttgttttcg cagtgattca tccggctgat
     5101 aagctgatta tagcatattt tttccaaatt ataattttat gacgaataat tcgtctttaa
     5161 ttacgatatt tacgtcataa atttatcaat agtgtttctc aatacccatt cttggtttag
     5221 cctcagatat ggcacactgt caccggagcg gctggctgat actgcccggt cgctgggcat
     5281 ccgggctatg gcattgaccg atatcaacaa tacatcctgt gcctggcaat atatcgaggc
     5341 ctgtcgtcgc aacgatgtcc ggccgattct tggtattgaa tttcgccggg acgacggtcg
     5401 tatgctatat gttggcatag cccgtaacaa taccggttgg gccaatcttt gccgcatgct
     5461 ttcggaagtg tcgctcgatg gaaagagcct acctgcatgt gcgccggagt tggattccgt
     5521 gtatttcatt taccgcaatc agccaaagga tatacgtacg ttccgtgatg acgagtttta
     5581 cggcattcgt cctgatgagg cacgggcact gtactcttcg cccctgcgta agcatatgga
     5641 caggttggtt gcatttacgc ccattacctt tgccgatgag gatggctaca agatacacaa
     5701 gctgctccgc tgtatcgacc gcaatattct tcttgggcag ttacgccaac aggattgtgc
     5761 gggttctgat gaggtattta tgactgaggc ggcattgcgt gatgcctttg ccttgtttcc
     5821 ggacatcatc cgcaataccg aaatgctgac cgactcctgt tccatcgaca tgacggcctc
     5881 aaaaatcaat aaccggcaat gttttaccga cagcaaggac gatgattaca ggctgcttgc
     5941 caagcttgca gtcaatggtt gtatccgtcg ctacggacgt cacaatgagc aggccaacaa
     6001 gcgcgtcgat tctgagctca gggtcatccg ggagcttgat tacagtgcct acttcctcat
     6061 tacctgggat atcgtgcggt atggtcggag tgtcggttac tttcatgtgg gccggggcag
     6121 tggagccaat tccattgtgg ctttttgcct tggcatcacc gatgtggatc cgctggagct
     6181 ggatctgtat ttcgagcgct tcatcaaccc gcatagaacc tccccgccgg actttgatat
     6241 cgatttttcg tgggacgaaa gggatgatgt gacggactat atcttcaaac gctacggcag
     6301 agaacatacc gcactgcttg ccacctacaa taccttcaag ggcaaatcca tcatccgtga
     6361 gctgggcaag gtagtcggtc tgccgaagag cgatattgac gtcatcgtgg atcatccgct
     6421 cgataccgac cgccatcatc cttttggcaa gcatctcttt cgctatggta agatgatgga
     6481 gggcatgccc aactatttgt cgatccatgc cggcggtatc ctcatcagcg aacggccgct
     6541 ttacgagcac acggcgctga aactcatgcc caagggcttt ccggtcacac atttcgatat
     6601 gtacggagcg gaagacctgc actttcataa gtatgatatc ctcagccaac gcggattggg
     6661 gcacatcaag gatgccattg acatcatcgc ccgcaatagg ggccgcatcg tggacatcca
     6721 cgacatatac cgcatcaagc acgatgaggc tgtccgggca caactccgaa gtggccactg
     6781 caccggttgt ttttacatcg aatcgcctgc catgcgtggc ctgatcagta agctgaagtg
     6841 cgataattat gtgcatcttg tggctgccag ctccatcata cggccgggtg tggccaagtc
     6901 gggcatgatg agggagtata tccagcgggc acatcacccc gataaggtgc aatatcttca
     6961 tgctgtcttc gagcagcatc tggcagagac atttggtgtg atggtgtatc aggaggatgt
     7021 gatgaagatc gtccatcact ttgccggcct tggcctggac gaatccgatg tcctgagacg
     7081 catcatgaca ggtaagaaaa agaattcgga caccttccgc gacctgcagg ccaggtattt
     7141 tgacaattgc agggcacgcg gctattctga tgcgttgacc aatgaagtat ggcgtcagat
     7201 cgagagcttc agcggctatt cgttctgcaa ggctcattcg gcaagttttg ctgtcgaatc
     7261 tttccaaagc ctctatctca agacttatta tccgatggag tttatggtcg cggttatcaa
     7321 caatttcggt gggttctaca gcactgagtt gtacgtacac gaagccagag tgagcggcgc
     7381 cactatccat ccaccctgtg tcaatcatag cgaccgactg accaatatcc atggaacaga
     7441 catccatatc ggcctgggcc tgatccgtga tctggaatat accaccattc gcaatattct
     7501 gaaagcgcgg gcagaggatg gtccgtttcg tgggctggaa gactttgtca gccgtgtggc
     7561 catcgatgcc acgcaactca ccctgctcat ccgcatcggc gccttccgct ttacagggtt
     7621 gaacaagtat gagctgatgt gggagaaaaa cactgtgtat aacgccagac atcgcgagac
     7681 ggtgggcatg ccggtgttgt ttgagccgga gcatgaacaa ttttccttgc ctgtgatgac
     7741 ggaggtggat tatgagcagg cttttgacga gattgaactg ctcggatttc cgttgacatc
     7801 gccttttgaa ctggtcgagg atgagcttcc cgcacgtatt cctgcacagg acatgatgcg
     7861 tcatgccggt cgcagggtta ctatggttgg gtattatgtt acccgtaagc cggtgcgtac
     7921 cgtaagcaag acactgatgt atttctatac ctgggtggac gaggctggcg atttttttga
     7981 taccgtacac ttcccgccag ccgtcaaccg ctcgcctttc aaaggctccg gctgttatat
     8041 catccgggga aaggtggtgc ccgacttcgg attcccgagc ctggaggtcg acagcatgga
     8101 aaagatgccg tggatcaagg atgggcggta ttgagcggag atggcaataa tcgattgaag
     8161 ttggataccg aggtccggtc cggatgggat ttttatatag aaaatagtca atgaaagaat
     8221 aaatgattgg gtatgaccat ggtgcccaaa aaataaaaaa atgtaatttt gaggaatatt
     8281 tgcatcggac aatttatgga tacataacat cctctatatc tttggccaca aaaattaaaa
     8341 atcatgatac gtacactttt attctttttt attacatgta tttcggtagc tgtatttgcc
     8401 caaccaccgg caggagacgc caatgtaggt gactattatg gcaagaagat caaggcgaaa
     8461 ggcgcgatgg atgttgccgt gctgcccgcc aaacttaggc ctgatgcccc aatcaaggtc
     8521 aaagtaaaag gaacggtact ggatgcatgt gccgagaaag gctgctggat ggtgatgaag
     8581 atcgatgata ctaatacggt aagagtgagg atgcaggatt atgccttctt cgtaccaata
     8641 gccatcatcg gcaagaccgt ggtagccgag ggagaggctg aaatgtccgt tctgtccgtc
     8701 aaggaacaga agcactttgc cgaagatgcc aagaaatccc aggaagaaat tgatgcgatt
     8761 actatgccga aaaaggaaat cagtctgatg gcttcgggca ttcttgttgt ggagtaattc
     8821 gggaaatgcc ggatagtact ctcccgaagt tgattctccg ggaagttatt tcattttttt
     8881 acgtttaaaa cttttacctg cttaatgggt tgaataacta ttcattaaga atttttaggg
     8941 taattgagat tatctttttg ccgttattgc atgcggaaac ctgaaaatta gattttctta
     9001 tcgaaaaatt atataaaatg aaacatgcgt tcaaagcttt tttacttttg atgatattct
     9061 ttattgtcgg ttcagtcaat gcacaaaaca gaagtggtga tgatggacag gcttccatgc
     9121 gagcggcaaa aatcaagagc gccaaaaaaa aggatcaatc aacccaacaa aaatccaatc
     9181 ccgcacatgc gtctttgttc gataacctgg atacgaataa agatggcaaa gtgagccggg
     9241 aagaagccaa taaaagtaat ctggtaatca taaaggagaa atttgataac ctggatacca
     9301 ataaggatgg ctttctttca aaagccgagg tccaaaatgc cagatacaat gtcaataagg
     9361 gacagcagcg tacgacatca aaagtaaaaa gcagcactac caataccgca gccaaaaagc
     9421 aatgatgata accggttggt ggtgattgtc tgtaaccaat aaggatctgt cgcaccggaa
     9481 aaggtgccgt tttgtagtga atgcgacggc accttttttt cttgctcact ttttaggagt
     9541 gttgaatctg tctgttggtg ttcaattcaa aattgaaaat tgccggaatc gatatacgaa
     9601 gacgtctctt ttccttaact tcgcccgtca atcagactat ttattatgtt gcaacgaatc
     9661 cgggtcagaa attatgccat catcgatgag gtcagtattg aattcgaccc gcatttcaat
     9721 attattaccg gtgagacagg tgccggaaag tccatcttac tgggtgcgct gggattgatt
     9781 ttgggcaatc gtgccgacag caagaccttc ttcaatactg aagagaaatg cattgtcgag
     9841 gcagaattca gcataggaag ctacagcctc catccctggt ttgaggccaa tgatctggat
     9901 tatgaggatc tgacgttgat ccgcagagag attcaaccgg caggtaaaaa ccgcaccttt
     9961 atcaacgata cacctgtgac cttgcaacaa ttgcaggaat ttacccagca actgatcgat
    10021 atacaccaac aattcagcct ctatgacata cagaaaccgg cttatcagtt gatgctcttt
    10081 gatgcataca gcggcaacaa ggtgttgatg gggcaatatt ctgaacttta ccggaaatat
    10141 attgccctcg gacgtacatt gaaagaattg caggaagaac ggcaaaaatc gttacgagag
    10201 gcagattatc ttgcttttca gctggaggaa ttgaatagtg tggattttgt caatatggat
    10261 caagacgcac tggaagaaga actcaacatg atcgaccatg cgggtaccat caaatcaacc
    10321 gccggcgcag cctatgttgc actttatgat gacgacaaca atatcatttc agcgatagag
    10381 gacattctca acagttttgg tccaatcacg gcagtctctc ccagaatcaa ggaggtggtt
    10441 gacagacttc agtcctccag gatagagttg agagagttgg cagaggatat caaccgactg
    10501 gctgaaagta cggagattga tgaagagaga gctgcagaga tcagggagtc gctggatcag
    10561 ttgtataaat tgcaaagcaa gcaccaggta ggggatatag ctggtcttgt tgccttgaag
    10621 gaggaaatag atggcaagct gaataattac tccaaggtgg atgatgatat tgtacggata
    10681 gaacaagaga tgagtcatca tgaagaagta ttgaacagga tggcagaaag tctttcagaa
    10741 tcaaggaaaa gccatattcc cgacttcatc gacacactta agggactgtt gtcggatctg
    10801 ggtatgcctc atgccacatt tgtaattgaa atggaaccat ccacggctct tacaccatca
    10861 ggaaaagatg ccattcgatt tctttttgca gccaataagg gtggtcagcc tgcggccatc
    10921 aaggatgttg cctcaggagg ggagatatcc agacttaccc tttcgatcaa gtctatggtg
    10981 gccgcagcaa ttccgttgcc ttccctgata tttgatgaga tcgataccgg actcggtggt
    11041 gaaattgcac tgaaaatggc tgaaatcatg cgcgaaatgg cgcgtgagca tcaactgata
    11101 gccatcacac attcaccgca gttggcttca aaagcagata aacatttttt cgtttataaa
    11161 gaaatagaag acaataatac atttactcgc attaaggaat tatctttgga tgagagaatt
    11221 gagaatatag ctatcatgct ttcgaccaaa ccacctacca aggcagcggt cgagaatgca
    11281 agagagcttc tggaaagaaa gtgattaatc aaaatattaa aagagaaaga attatggcaa
    11341 atggactgtt gaaaggtaag aaaggcatca tcttcggagc gctggacgaa aaatcactcg
    11401 cctggaaagt tgcagaaagg gcttatgaag agggcgcttc cctcgtattg acgaatgcgc
    11461 cggttgcatt gagattcgga caaatcaatg ccctggcaga gaagaccgga gctcccgtta
    11521 tccctgccga tgccacatcc agtgaagagc tggctaagtt gatcgatgac agtataaacc
    11581 atttcggtgg caaagttgat tttgtattac attccattgg tatgtccctc aatatgagga
    11641 aggaaaagca ttatacggat ctcaattacg attttatgca taagactctg gatatatccg
    11701 ccatttcctt tcaccggatt ctccatgaac tgtatgctaa gaacgctgta gccgattggg
    11761 gttccatcgt tgcactgacc tatatcgctg cacaacgtat ttttccggat tacaacgaaa
    11821 tggccgatgc aaaggctctt ctggagtcta tttcccgcaa tttcggctat catttcggca
    11881 ccagaaacaa agtacgggtc aataccattt cccagtcgcc aaccatgacg acagcaggcg
    11941 gtggagtaaa gggatttact gactttttct cttatgccga taccatgtct ccccttggaa
    12001 atgcgggtgg tgatgctttt gccgattatt gcattaccct gttcagcgac ctgaccaggt
    12061 atgtaaccat gcagaatctt tttcacgatg gtgggttctc cagtatgggt atcaacccta
    12121 agttgttggg agttgagtaa tccgccgatt tttttgaaaa aaaggttatc aaaatatctg
    12181 tttttagagt ttaatcatta aatgcagatg ttgatccatt tattgttgag agactgtatt
    12241 ctgtaatttt aaatcaatca tcacctccta atttgtttaa ccaaaaaatg gtttcaaatg
    12301 aaatcgatga gtaaattttt aattgctttt attttttttc ttttcaccgg cgcttcgtta
    12361 actgcacagg acttttttag cattacgact gaaagcaagg ctgactttat tgacaagaac
    12421 aaggagtata tcaagccgga gcggaaagta ttcttcaatt acgattacaa ttcgcttcgt
    12481 agtatactta ggacggcacc gcaggaaagt tattttgata gaggattacc cggcttaaag
    12541 ctccatctgc ccaatcctga aggcggcctt acagagttcg atatttacga atccccaatc
    12601 ctcagcgaac cggaatactc ccaatttcca aatatccgga cctacatagc agtgaatcaa
    12661 gcgaataaaa tagagcatgg caggatagat ataacggagc atggctttca tggcttgata
    12721 tttacagatg gtgatgtgta tcttattgaa ccggcctcgt taggcaaccg gcaatcttgt
    12781 attacctatt ataagaagga caataagatt cgacccgact ttgagtgtat gactgaaggt
    12841 tccggtttta atgacgtaga tctggagacc cggacaccac ttagttgcaa cagtagcatg
    12901 acattggtgt catacaggac tgctgtagcc tgtacatggg aatatagtaa ttttcatggt
    12961 ggcactgtag ctcttactct ttccgccata acgactactg taaatcgtgt gagtgctgta
    13021 tatgaaaaag atatgggtgc aaggctgatt cttattcccc aaaactcctc tatcctatat
    13081 accacagagg gtggatatac cgcttcaaca gatccttaca ccaatgatga tgggggctta
    13141 atgcttggtg agaatcagac tgttctcaga agcgtaatcg gcgcacccaa ttatgacatt
    13201 ggccacgtgg ttagtaccgg agggggtgga gtagcttggt tagctgttgt ttgttttaat
    13261 acaccgggaa ccaataacag taacaatgca aatcttaagg ctctaggtgt tacaggtgcc
    13321 tctacgccgg taggggatta ttttgatata gattatgtcg cccacgaaat aggccatcaa
    13381 ttcggagcca atcatgcctt caatggtaat accggtagtt gtagtggtaa tcgtaatggt
    13441 tcaacggctt atgaacccgg tagtggttct acgattatga gttatgcagg tatttgtgga
    13501 gctcagaata ttcagcctca ttcagatcct tactttcact gggtatctat gggcgaggct
    13561 ttgacgcata tcaatacaac aacttcatgc cgaactgctg tttcgggtac caataacaca
    13621 accccgcaga ttacaagttt tacaacgggg aaaaccatac cggccaatac tcctttttat
    13681 cttgacgcct cggccacaga tgccgatggg gatgcgataa cctactgttg ggaagaaaat
    13741 gatttgggca ctgccggtaa tattaatgct tcatctacta ctgcacccat cttccgttca
    13801 ttcaatcccg tcagctcagg gagaaggtat tttccgcgac tgcatgatgt attgaacaat
    13861 accacgacat atggcgaggt gttgccttcc gtggcccgga cacttaagtt tactatcaca
    13921 gcaagggata atcatgctac aggcggaggt atctgtcgcc aaagctcaac cattacagta
    13981 gttgccagtt caggatttaa ggtgactgct cccaatgtgg ctacaacctg gagcagtcca
    14041 ggtacgggca ccatcacatg ggatgtagcg ggtacggcat cctcgccaat tagctgtgcc
    14101 aatgtcgatg ttcaggtttc ttttgataat ggtctctcct ggacgtctat cggtgtagga
    14161 gtgcccaata atggcagttt tatctggacc atcccatcgg gtatcaattc tagtgatgtc
    14221 cgttttcggg taaggtgttc agataatatt ttctatgcag tcaacacggt acccatcacc
    14281 attggaaatc ctgtcaatac ctgttatacc tacactaata attctacctt gaccataccc
    14341 cttacaccga gtacagtcac atcctcaatc attataagta accgcgccgc tgacgaaatc
    14401 gtgaaggata tcaatatcac caatctgaat gtaactcact ccaatcttcg ggatcttcgc
    14461 ttcaagctca aatcgtccaa tggtgcgtcc aatattctta tggacagtaa atgttcaggg
    14521 tacgctaata tcaatatagg gtttgacgat gaggctgtat ccggtacttt gccttgtcct
    14581 ccaaccaatg gaggtgccta caagcctcag gaattcctga atgttcatga cgggatgccg
    14641 gccaatggtc aatgggatct ggagatagat gactggtatt cgggcaatgg gggatcactc
    14701 aatagttggt ctgtccaaat atgtgttgaa aaaaagaccc cactgccgtt gcatttgttg
    14761 tcatttgctg cagaagctgt taaaatcggc gtttacattg gatggactac gtccaatgaa
    14821 agggaggtgg aagtatttac tgtggaaagg atggctcacg atggaagctt tcggtctatc
    14881 ggtcatattc ccgcaaaaaa tattgacgcc atctccaatt atggcttagt ggatgagtat
    14941 ccggacaaag gaatcaatta ttaccgattg aagatgattg gaaagaatgg aaaggtaagt
    15001 tattcagaga ttaagagcgt aagatttgag ggaaggtcaa gcgtgacaat atttcccaat
    15061 ccgacaaatg ggatactgtc aataaaaaga attggaaacg aaggagagat tcattccata
    15121 aacatacttg acctgtccgg tcgtgaagta atgtcacttc ctatgcgcct tcatctatat
    15181 gaattaaaag atatcgatgt gtcctccctg attcccggca tgtatctggt gcaggttaat
    15241 atgaatacgg gagtggagac cataccattc attaaatctt agatcttaac cttgttaagc
    15301 tgagcatacc atatggatgg atgatgatgg ctcagataga ggaaaggtgc agatctcatt
    15361 ggcagagggg ctttggacat taaccttcct gattgaaggt ggcgtccatt ttgatgaatg
    15421 ctgcaagaag caattgcccg gtcgaatgta ttatccgttt atcaaggggt tgcagaagag
    15481 atgttacagt tgatgtgttt atatgcaata gctgagataa tattcatcaa ggatcgatag
    15541 tagcatccgg ttatgttatg aacaatttgt ctttgggcca aggtaaatgg ttgttctcat
    15601 tcattttggc atgatgactt attgacctgg aatttcaata aggatatcat tgaaaagcaa
    15661 tggaattccg gtaaaacaat ttaccgaatt aatagaaatc caatggtaca gtgatttacc
    15721 aatacaaaag tgcaggctct cgttttggag tttataataa atcatttagt cggaactccc
    15781 actggatggg gtatattata tcttcaatga gataatttcc aaaaatatct tttgattttt
    15841 gattctttta acagatattt atatatttat tgcttacaag gtaaaataat cggcaattat
    15901 atgataatat ctgtactcag agaatcaaag gacggagaaa gaagggtcgc attgacacct
    15961 gatgtgatca aattactgat aaaaaagggt tttcaagtca aagtagaatc cggtgccggg
    16021 tcgaattcgt ttttttccga tgatgaatat gtccgcgcag gagccgtaat cgcatctacc
    16081 aggcaagaag cttgtagtca ggacatcatt ctaaaggtca atgctccctc cactgaagaa
    16141 gtttctatga tgaaaaccgg cgcatgttgt atttccctcc tttatggata tactcagcct
    16201 gtgttactgg atgctttcaa tgcgagatcc attacatcct ttggtatgga tgcaatcccg
    16261 cggatttcaa gagctcaaaa aatggatgcg ctcagctctc aggcaaattt agcaggttat
    16321 aagtcggtaa tccttggatg tgatgccttg agtaagattt ttcctttatt gatgactgcg
    16381 gcggggacta ttaccccatc taaggtcttg atcttcgggg ccggtgtggc ggggctccag
    16441 gcagtagcca ctgctcgaag acttggagcc at
//
LOCUS       DAOQQN010000013        16407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1103_length_16407_cov_5.770854, whole genome shotgun sequence.
ACCESSION   DAOQQN010000013 DAOQQN010000000
VERSION     DAOQQN010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 16407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16407
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1103_length_16407_cov_5.770854"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PLG24_00995"
     CDS             complement(<1..522)
                     /locus_tag="PLG24_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23485.1"
                     /translation="MSEEITKGSDDSENLQNDMPDQVNESINAEASEISEATDVTLDA
                     ENETLEEGSESGGEQIGKAEDNFTEGEGQLQEKLGDAGAQISGFAKTAQHKAGEVLSE
                     VGETLGEWKDKVTEKAAAVKEDASEFFSGAGSKASAFAQEAKEEISEFAQEAKETLSE
                     WKEKVAEKTADMRE"
     gene            complement(894..1301)
                     /locus_tag="PLG24_01000"
     CDS             complement(894..1301)
                     /locus_tag="PLG24_01000"
                     /inference="COORDINATES: protein motif:HMM:NF016153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HMZ23486.1"
                     /translation="MRFLAPFHSSVNAITFLVLLFAFYQIKQKNIKGHQKAINLALVL
                     SCIFLVSYVLYHITTPERKFCHEGVIRIIYFFLLISHIVLAAVILPFILFTYTRAFTG
                     QYERHKKLARYVMPLWMYVCASGPLCYLMLHDC"
     gene            complement(1432..1965)
                     /locus_tag="PLG24_01005"
     CDS             complement(1432..1965)
                     /locus_tag="PLG24_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23487.1"
                     /translation="MKQGIEFRQTALKELAQNIQVDSCSPMVFQSSILPLDSMKGKVW
                     IFIDKDEIQPGLLAKILKTLFAQNSGKGSLGIVLCSIDSTMVPDVPEYIYQSGFALMG
                     KHGNFDCLKSSFTRRDTQASNHLNYNTLLMDVYGKVRNGYELAQDEDVRELIKHVTVL
                     LPEYKREKPVLIRQQGY"
     gene            complement(2085..2546)
                     /locus_tag="PLG24_01010"
     CDS             complement(2085..2546)
                     /locus_tag="PLG24_01010"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HMZ23488.1"
                     /translation="MSLSPEEGKRVVYKGMILLAVITLIEVFIALIGNGHVIDGFHLP
                     KWVMYPAMIMLSLYKAYYIISEFMHMKYENKGLVISASWPIILIVWAMIAFLNEGGAW
                     HDRKAYVELVNSKGYIKPSEASLETFHNEVGPKDDVTPTEGSAKPTETNQH"
     gene            complement(2568..3446)
                     /locus_tag="PLG24_01015"
     CDS             complement(2568..3446)
                     /locus_tag="PLG24_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HMZ23489.1"
                     /translation="MTTDTHEHAQVGHHDNASGPELWEGGREPFKASYGKLMMWYFLV
                     SDAFTFSGFLIAYGALRFSVPTWPLPDFVFSTFPGGFHHAPLIFVTVMTFILILSSVT
                     MVRAVQEGKRENKKGVVKWMGLTIIGGLMFLSCQAWEWTNLIAKEHMTITTNPFGTHT
                     TDGVYLNPDGTEAKKENGQPDSFKAGTTYLIHKKDGEWHKRVFKITSGEYKGQIAQEA
                     FGPKAFGGLFFFITGFHGFHVFSGVVFLLVIMLNVMSGLYEKRKNGYEMVEKIGLYWH
                     FVDLVWVFVFLVFYLL"
     gene            complement(3479..4048)
                     /locus_tag="PLG24_01020"
     CDS             complement(3479..4048)
                     /locus_tag="PLG24_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HMZ23490.1"
                     /translation="MEVTLSQKKGIQPLKFALWSGFASIIMMFMALTSAYIVRQAAGN
                     WLEFKLPTMFLYSSLTILLSSVTMHMSYTGFKKGNEMNYKLFLLATLALGLMFIMFQI
                     QGWQAMHIIGINLDGNPSGSFVYLISGLHAAHVIGGIAILIVALLQAFSLKYSVTPKR
                     LLRFQMTAQYWHFVDILWIYLFIFFLLIR"
     gene            complement(4050..4946)
                     /gene="cyoE"
                     /locus_tag="PLG24_01025"
     CDS             complement(4050..4946)
                     /gene="cyoE"
                     /locus_tag="PLG24_01025"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HMZ23491.1"
                     /translation="MKENYLNSTGVAQSSLLKDYALLFKYKLTSFVVFSAMMSYLVSI
                     DGAINWFSFMVLAVGGFCITAAAHSLNEVLEKDYDKLMKRTSNRPVAAGRMTSSHAVF
                     VAGVMWLFGVICLFYFNILAALLGSLAVVSYAFVYTPLKRYSTIAIPIGSLPGALPLL
                     IGNVAAIGYIDQMGIYLFAIQFIWQFPHFWAIGWLGYEEYKKAGFKLVASKDGGCDPN
                     IGLNSAFYALLFIPLAFFGFYSSLIGIPALVVTILASIGFAYYGYRLYRDRTKKAALN
                     LMFASLFYLAIALGAVYIDKLF"
     gene            complement(4986..6845)
                     /locus_tag="PLG24_01030"
     CDS             complement(4986..6845)
                     /locus_tag="PLG24_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HMZ23492.1"
                     /translation="MSDHNITVKDEVDDIIEKNGYDDHFHEHHGEDKYKSGFIGKYIF
                     SLDHKIISRQFLITGIIWAIIGAAFSVFFRLQLGFPESDMSWLKPILGKWIIINDAGV
                     GALSQEFYYALVTMHGTILVFFVLTAGLSGTFSNFLIPLQLGARDMASPLLNMMSYWF
                     FFLAGVVMLSSFFLSTGPFSGGWTAYPPLSALPQASSGSKGGMNLWIISMTFFIVSQL
                     LGGINYVTTILNLRTKGMSMWRMPLTIWSFLITAILGILTFPVLFSGVLLLLFDRSMG
                     TSFYLSEIFIQGAALENVGGNPILYQHLFWFLGHPEVYIVILPAMGMVSEILSVNSRK
                     PIFGYKAMVFSMMAIAFLSCIVWAHHMFTSGMNPFITNFFVLFTLIIAMPSAIKTFNW
                     LTTIFRGNIRLNSPMLYCIGFVSLFISGGLTGIWLGSSTLDIQLHDTYFVIAHFHLVM
                     GVAAFFGMFAGLAYWFPKMFGRFMNESILKIHFWVTLIGAYCIFWPQHYLGLAGVPRR
                     YYRFDSFDTFAGWEGVNAFISISAIVVFFVQLLWVFNFFYSIWKGKKVTEKNPWHANT
                     IEWTTPIDAPHGNWPGKLPTVERWSYDYSKDEDAEQDFITQITPYAPGEKVEH"
     gene            complement(6880..8043)
                     /locus_tag="PLG24_01035"
     CDS             complement(6880..8043)
                     /locus_tag="PLG24_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HMZ23493.1"
                     /translation="MTALAIIGCILLVGLVIYQIARINEIIKRIKGEEKAFLQSNNNT
                     AVAILVFGFLLLFLTIYTSYHYKNMMFGYGPMLSASEHGTLIDKSMHIMLILTGIVYI
                     ITHILLFYFAYRYRYRKGKKAWYYPDNTKLEMVWTLTPFFVLAFLAIDGLMVWNKMMA
                     DIKPDEKHIEVEVTGMQFAWIVRYPGIDNILGTRDFKLTSGTNDLGQDWNDPKNWDDF
                     KPDEIVLPKGKKVRIRILSRDVLHSFFLPHFRVKMDAVPGMPTYFVFTPSMTTEEYKN
                     NLKKYPEWNVPADPSDPESKLKWEAFTFELACAELCGDSHFAMRRPVRVVTPEQYDAW
                     VKSQQSYYLANVRGKDDDPNKGKPITINGVVNNGPAPSAEGGSETKADSTKAE"
     gene            complement(8077..9351)
                     /locus_tag="PLG24_01040"
     CDS             complement(8077..9351)
                     /locus_tag="PLG24_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23494.1"
                     /translation="MDQFIFKSKYKNTLIAFMVIGLISMVITWFQDDALHTRFWSNYL
                     HNTMYFTGIAFAALMLMTMGILTYGSWWVAFKRLWEAYSMFLITGLILMLVVVGGVWG
                     HYHHLYHWTDAEDVANDLILKGKSGFLNPYWYTLGTVIVIGAWAYFAIKLRQLSKEQD
                     NNNGFDDIKEYKRMKVLSSIFLPIAGFSSAAMIWQWIMSLDAHWYSTLFAWYATASWV
                     VACFAFTALLIIFLKGQGFINFVTVEHLHDVGKFVFAITVFWTYLWFSQFMLIWYGNN
                     GEETTYFNRQMQQYPVLFWGNLVINFVLPFLVLLRNDTKRKIGSMVFVCILVIFGHWW
                     DFFLMIKPGTLHTAHEALAKMEGGHGAVAEGAEHASNFVSGFTLPGLLEIGTMLGFFG
                     LFVYVTFVQLGKLSLKPKHDPLLPESLHHHVQ"
     gene            complement(9406..10206)
                     /locus_tag="PLG24_01045"
     CDS             complement(9406..10206)
                     /locus_tag="PLG24_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMZ23495.1"
                     /translation="MKYNRYLIFSGLAFIAASLSLESCAPAGGNKPGHEYMQDMGHSI
                     AYEANMVDLFHYNRWDMNGYIKLAQPRNPVMGTIPRGFVGLAKYSNSEIPASVEAEFA
                     NETVNGSVPFYYGDTEEDRKRAENDIKLNPYPITKENLAHGKELYDIYCGICHGEKGD
                     GSGYLVRDDGKYPAQPAILTSDEFIAASNGRFYFAIMHGKNVMGSYSDKLSYKERWQV
                     IQYIRSLQAKTKGLKYDETENTLNNTDFIASAKKAMPAVADTMPTVAN"
     gene            complement(10230..10760)
                     /locus_tag="PLG24_01050"
     CDS             complement(10230..10760)
                     /locus_tag="PLG24_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HMZ23496.1"
                     /translation="MASKNKEVLFGLFDDETQLLSAVADARKSHMEIDDVYTPFPVHG
                     LDPLLGLEESRLHIAGFFYGLTGTATAFFGISWIFTRDWPTIFGGKPYFSFPAFVPIM
                     FELTVLFASIGMVVTFYTICGMGPGVKAKTLHDRITDDKFCISFDTSDASDEQKSGLR
                     AFFSKHGASLVDTKFI"
     gene            complement(10785..12215)
                     /gene="nrfD"
                     /locus_tag="PLG24_01055"
     CDS             complement(10785..12215)
                     /gene="nrfD"
                     /locus_tag="PLG24_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HMZ23497.1"
                     /translation="MSIVVSPTREPLIFGGKTYHQITEDICTPSEKAPTRQWLIGFII
                     AVFFLAVFVFCVGWQIYYGVGAWNLNRTIGWGYDITNFVWWIGIGHAGTLISAILLLF
                     RQKWRTGVNRAAEAMTIFAVICAFMFPVIHVGRIWLLFYFIPLPNTRGPLWVNFNSPL
                     LWDVFAISTYFTVSLLFWYTGLVPDFATLRDRASGFRRKLYNFLSFGWTGAAKHWQRF
                     ESLSLVLAGLATPLVLSVHTIVSFDFATSVIPGWHTTIFPPYFVAGAIFSGFAMVQTL
                     MLVTRKALKLEDYITINHIESMNKVILLTGTVVGIAYLTELFISWYSQYVYEQFAFAN
                     RAAGPFWWSYFGMMACNVLSPQVFWSKKIRRSLWWTFFMSIFVNIGMWFERFVIIVTT
                     LARDYLPSSWSYYYPSWNEIGIYMGTFGLFFTLYLIFVRIAPVVAIAEVKSILKSSGD
                     QYRGKNRGYNEKNEVVVAHSDHEEHH"
     gene            complement(12278..15469)
                     /locus_tag="PLG24_01060"
     CDS             complement(12278..15469)
                     /locus_tag="PLG24_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HMZ23498.1"
                     /translation="MSEFDQGYWGSSEEFTNDEAFVKGLHDEFYELPILNNMSKEEDE
                     SAKGHNRRDFLKYLGFSIGAATIAASCETPVRKALPYVKMPDSIVPGVANYYASTFIK
                     GNDVLPVVVKTREGRPIKIEGNTLSSFSGGGTSARAQASVLDLYDTGRYKFAGKSDGK
                     GGIKKMKWSELDKAVMDKLASGGNIRIVSNSITSPSMNAVINQFITAYPTAKHVTYDP
                     YSSSATLVANERSFGKRVFPGYDFGNAQIVVNFGADFLGTWVSPVEYTNAWTKNRKIK
                     NFDKPEISRLIQYESNMSYTGSNADNRLLIKPSEQGLAIAKLYNAVASLTGGSQLSVS
                     GTLKNQKAEKSFAAVAKNLVDNKNAGKASLVLSDSNNIAEQIIVNKINDLLGNYGRTI
                     DLNNYSNQRKGIDSDIVGLIKEMNAGSVSAVFILGDANPVFDLPEGKDFAAGLKKTGL
                     SVSLSTVPNETSAACGYVAPSNHFLESWGDAQPRKNNYYIMQPTIAPLFATRQAEQSL
                     LAWAKTPVDDFSTYMQGVWRSSLFGLQSEYSDFQRFWDNTLHNGLIELASQSGAGSVA
                     VDVSGLGSAVTKPGSGLEVKYYEPIGVGYGAYANNPWLQEMPDPVTRTVWTSIIHIPL
                     SWDGDSKFNYYQNLKKDGLFVDLSSGKTAVKDVPVVRQFGQMAETFAMPVGYGRTHGN
                     ASSMGVGNYIINTLTRDADGNLRYWNDNVKVEPNGGEDKHFASVQLHHTLGVRGKDKS
                     NKEINVDEKAIMTIGSGFQGGLTDRSILKSADLSNFAEKVEKLKEFRATAAKLNNETL
                     YPGFDHLYKQGHHWGMSIDLNACTGCGACQVACISENNVPIVGKKEVHRQHEMTWLRI
                     DRYYYGDVESPNVFYQPMMCQHCDNAPCENVCPVNATNHSAEGLNQMAYNRCVGTRYC
                     ANNCPFKVRRFNWADYTTADLFGRNELNVMPDGESLFYEDNLTRMVLNPDVTVRSRGV
                     IEKCSLCVQRIQEGKLRAKAEMRPLKETDIKPACATACPAGAITFGDQNMKDGILNKE
                     WDEPTTYFVLEEINVKGKIGYKMRVTNKNADLS"
     gene            complement(15514..>16407)
                     /locus_tag="PLG24_01065"
     CDS             complement(15514..>16407)
                     /locus_tag="PLG24_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HMZ23499.1"
                     /translation="NLKNVEREKEGQPVELKTIVQLLTSKSVIGVVLFALIVLGSFTT
                     VNNAINLGRQQNYAPEQPIKFSHVTHAGINKIDCQFCHDGARRSKHSVIPSTNTCMNC
                     HKAIKKGSTYGTQEITKIFASIGFDPNTDQYIENYEKMSQKDIAKIYKKWIGNQYLTE
                     EGHSMDAEGKEFVKNQWDHIVSSLTNDHKKKIQGPIEWVRVHNLPDHVYFNHSQHVKV
                     GKVDCEACHGKVAEMETLRQYSPLSMGWCINCHRDTKVQFNENPYYDSYVRYHQELKD
                     GKRDKVTVADVGGLECQKCHY"
BASE COUNT         5110 a         3713 c         2840 g         4744 t
ORIGIN      
        1 ttcacgcatg tctgctgttt tctctgcaac tttttccttc cattcagata aagtctcctt
       61 ggcttcctga gcgaattctg aaatttcttc tttagcctct tgtgcaaaag cacttgcttt
      121 acttcctgca cctgagaaaa actcacttgc atcctctttc accgctgcag ctttctcagt
      181 caccttatcc ttccattcac ccaaggtttc tccaacttct gacaagactt caccagcttt
      241 atgttgtgct gtttttgcaa atccgcttat ctgtgcacct gcatcaccta atttctcctg
      301 caactgtccc tctccttctg taaagttgtc ctctgccttg ccaatttgtt ctccaccact
      361 ttcactacct tcctccaacg tttcgttttc tgcatcgaga gtaacgtcgg tcgcttcgga
      421 aatttcggaa gcttcagcat ttatactttc attgacctgg tcaggcatat cattttgtaa
      481 attttcagaa tcatcagatc cttttgttat ttcttcagac atttgttaat aatttaattt
      541 agaaattttg gtatactttt ccaattgttg aatatgtatc atacgatata ttcacaatca
      601 ttaaactgta atattgtgat tttcgttcca cattacccca aaattcacga ataaaccaat
      661 aaattatcag gcattatcga atttcatttc tttagcattt gatttatagt aaattcattg
      721 attctacaaa ccataaaatc cataatggaa ggatatcatt caagactata atctcattcc
      781 ccaataaagg ctatcgatta tcatttctcg ttacgatgga aaactacttc actttttttg
      841 cctcaagata ataattttct tcgtatgaat ataaaacaga aacatcttat aattcaacaa
      901 tcatgtaaca tcaggtaaca taatggcccg gaagcacaga cgtacatcca caagggcatg
      961 acataccttg ctagtttttt gtgcctttca tattgcccgg taaatgccct tgtataagta
     1021 aataaaataa aaggtaatat aacagctgct aatacaatat gtgaaatcaa aagaaagaaa
     1081 tagatgatcc tgatgacccc ttcatgacaa aattttctct ccggagttgt aatatgatac
     1141 aatacatagg acaccaggaa tatgcaggat aataccaatg ctaaattaat tgctttttga
     1201 tgccctttaa tgtttttttg cttgatctga taaaatgcga ataaaagaac taggaatgta
     1261 atcgcattta cacttgaatg aaagggtgcc aagaatctca tatccattcc tacatcaaaa
     1321 tgaattctcc tcatgaaaat aattgttagg aagacaatca ggctgatcac ccaggctaat
     1381 ctatttaatt ttctttcctt cttttttgct tgggtatatt ccatctattt tttaataacc
     1441 ttgctgacga attaaaacag gtttctctcg cttatattcg ggcaaaagta ccgtcacatg
     1501 cttaattaat tcccttacat cctcatcttg ggccaattca tatccatttc taaccttacc
     1561 atagacatcc atcagaagtg tgttataatt caaatggttt gacgcttgcg tatccctacg
     1621 cgtaaagcta cttttcagac agtcgaaatt tccgtgctta cccatcaatg caaaaccaga
     1681 ctgatatata tactccggca catccggtac cattgttgag tcaatgctac ataggactat
     1741 gcctaaggaa ccctttccgg aattctgggc aaaaagggtc tttagaatct tggctaacaa
     1801 gccgggttga atttcatcct tatcaataaa aatccagact ttccccttca tggaatctaa
     1861 tggcaagata gatgattgaa ataccatcgg gctacatgaa tcaacctgaa tattttgcgc
     1921 taattcctta agggcagttt gcctgaattc tattccctgc ttcacataaa ccaaacttat
     1981 aaatggcaat aatatgaagc agcataaaat aacccaaatt actggtttct taagaatcct
     2041 gccaatactg ctcacacttc taatttataa taggagacag tttattaatg ctgatttgtc
     2101 tcggttggtt ttgccgaacc ttccgttggt gttacatcat cttttggacc gacttcattg
     2161 tggaatgttt ccaaagatgc ttctgatggc ttgatatatc ccttgctgtt taccaattca
     2221 acatatgcct ttctatcatg ccaggcgccc ccttcattca ggaatgcgat cattgcccat
     2281 acaatcagga tgataggcca cgatgctgaa ataacgagac ctttgttctc atacttcata
     2341 tgcatgaatt cagaaataat atagtatgct ttgtatagac tcaacattat catagccgga
     2401 tacatcaccc atttaggaag atgaaatcca tctattacat ggccattacc gatcaaagca
     2461 ataaatacct caatcaaagt aatgactgcc aaaaggatca tacccttata tacaactctt
     2521 ttaccttctt ctggagataa gctcattttt attaattttg atatttatta caataggtag
     2581 aatacaagaa aaacaaatac ccacactaaa tctacaaagt gccagtataa accaattttc
     2641 tctaccattt catagccatt cttacgcttt tcatataacc cactcattac attgagcata
     2701 ataaccaaca ggaatacaac acctgaaaat acgtggaatc cgtggaatcc agtgataaag
     2761 aaaaacaggc cgccaaatgc tttgggtccg aacgcctcct gagcaatttg acctttatac
     2821 tcaccagacg taattttaaa tactctttta tgccactcac catctttctt gtggataaga
     2881 tatgttgttc ctgctttgaa tgaatcaggt tgaccatttt ccttttttgc ctctgttcca
     2941 tcaggattaa gatacacacc atccgttgta tgtgttccga aaggattggt agttatcgtc
     3001 atatgctctt tggcaatcag gttagtccat tcccatgcct gacaacttaa gaacataaga
     3061 ccaccgatga tggtcagtcc catccatttt accacacctt ttttattttc ccttttccct
     3121 tcctgaactg cacgaaccat agtaactgaa gacaaaatca aaataaaagt catcacggtc
     3181 acgaagatca aaggggcatg atgaaaccct cccggaaaag tagaaaaaac aaaatcaggc
     3241 agaggccagg taggaacact gaatctcaag gctccgtaag caatgaggaa acctgaaaat
     3301 gtaaaagcat ctgataccag gaagtaccac atcatcaatt tgccgtaact ggctttgaaa
     3361 ggttctctcc ccccttccca aagctcaggt ccactagcat tatcatgatg acctacttgc
     3421 gcatgttcat gtgtatctgt agtcatttat tcttgttttt attgaattct aaaagatatt
     3481 acctgattaa taggaaaaat ataaataaat atatccataa gatatcgaca aaatgccagt
     3541 attgagccgt catttgaaat cgtagcaatc tctttggtgt tactgaatat ttcaaagaaa
     3601 aggcttgaag caaagcaacg atcaaaattg caattccccc aatgacatgg gcagcatgca
     3661 gtccgctaat aagatagaca aacgaccctg aaggattgcc atccagattg attccgataa
     3721 tatgcattgc ctgccatccc tgaatctgaa acatgataaa catcaacccc aacgccagcg
     3781 tagccaatag gaaaagctta taattcatct catttccctt cttaaatcca gtataactca
     3841 tatgcattgt aacactggat aataatatag ttaatgagct gtacaagaac atggtaggca
     3901 acttaaactc aagccagtta cctgcagcct gccgaacaat ataagccgaa gtgagagcca
     3961 taaacatcat tatgatactc gcaaacccgg accacaaagc aaactttaaa ggttgaattc
     4021 ccttcttttg tgataatgta acttccatgt taaaataatt tatcaatata aacagctccc
     4081 aacgctatcg ccagatagaa aagggaagca aacattaaat tcaaagcagc tttcttagtt
     4141 cggtctcggt acaatctata accatagtat gcaaatccaa tagatgccag tatcgtaact
     4201 accaacgccg gaatacctat cagggatgaa taaaatccga agaaagccaa cggaatgaac
     4261 aacaaggcat aaaaagcgga attcaaacct atattcggat cacaaccgcc atccttacta
     4321 gcaactaatt taaatccagc cttcttatat tcctcataac ccagccatcc aatcgcccaa
     4381 aaatggggaa attgccagat aaactgaatc gcaaaaaggt aaatacccat ctgatcaata
     4441 tagccaatag cagcaacatt tccgattaac aatggcaagg caccgggtaa ggaaccaatt
     4501 ggtattgcta tagtagaata tcgttttaat ggtgtataca caaatgcgta tgaaacaaca
     4561 gctaaagacc ctaataatgc agctaatata ttaaaataga aaaggcaaat aaccccgaaa
     4621 agccacatga ccccagccac aaatactgca tgagaagatg tcatccttcc ggcagcaacg
     4681 ggtctgttgg aagtccgctt catcaactta tcatagtcct tttcaagaac ttcattcaat
     4741 gaatgtgcag cagcagtaat gcaaaatcca cctacagcca aaaccatgaa agaaaaccag
     4801 ttgattgccc catcaataga aaccaaatag gacatcatgg ccgaaaagac cacaaagctg
     4861 gtaagcttat acttaaataa cagggcatag tccttcaata gactagattg agcaactcct
     4921 gtactattta aataattttc cttcacaatt attaggttat aatcagctca aaaacaatca
     4981 gcctatcaat gctcaacttt ctcgcccggc gcgtatggtg taatttgagt tataaaatcc
     5041 tgttcagcat cttcatcctt actataatca tatgaccagc gttcaacggt aggcagttta
     5101 ccaggccagt ttccatgtgg cgcatctata ggcgttgtcc actctattgt attggcatgc
     5161 catggatttt tttctgtcac cttcttaccc ttccagatac tgtagaagaa gttgaatacc
     5221 cacagcaact gtacaaagaa aactacaatg gcagagattg aaataaatgc attgactcct
     5281 tcccatccgg caaatgtatc aaaactgtca aaacgataat atctccttgg tacaccggcc
     5341 aaaccaagat aatgctgtgg ccaaaatata caatatgctc caattagggt cacccagaaa
     5401 tgtatcttta ggattgactc attcataaac cttccgaaca tcttcgggaa ccagtaagcc
     5461 aagccggcaa acataccaaa gaaggcagca acacccatca ccaagtgaaa atgcgcaatg
     5521 acaaaataag tatcatgcaa ctggatatcc agggtggaac tacccagcca gatacccgtc
     5581 aaacctcctg aaataaacaa actgacgaat ccaatacaat ataacatagg agaattgagc
     5641 cgaatattcc ctctgaatat tgtggtcagc cagttgaatg ttttaatcgc agaaggcatt
     5701 gctataatga gggtaaacaa tacaaagaaa ttggtaataa aaggattcat acccgaggta
     5761 aacatatgat gtgcccatac gatgcaagat aagaatgcta tagccatcat ggagaaaacc
     5821 attgctttat atccaaatat aggctttcgg gaattgacag acaagatctc cgaaaccata
     5881 cccattgcag gaagtatcac gatatacact tcagggtgac caaggaacca gaataaatgt
     5941 tggtataaaa ttggatttcc tcccacgttt tcaagagcgg caccttgaat aaatatttca
     6001 ctcaaataga aactggtacc catgcttcta tcgaatagca ataaaagtac gcctgaaaac
     6061 aaaaccggga atgtcaaaat acctagtatt gccgtaatca ggaatgacca gattgtcagg
     6121 ggcatgcgcc acatggacat gcctttagtc ctaaggttga gaatcgtagt cacataattg
     6181 ataccgccaa gcaactgaga aacaatgaag aaagtcatac tgatgatcca caggttcata
     6241 cctcccttcg agcccgacga tgcctggggc aacgcactca aaggaggata ggctgtccac
     6301 cctcccgaga aaggacctgt gcttaggaaa aaagaagata acataactac ccctgcaagg
     6361 aaaaagaacc aataagacat catattaagt agtggcgatg ccatatccct agcgcctaat
     6421 tgcaatggaa tcaggaagtt actgaaggta ccactcaaac ctgccgtcaa aacgaaaaag
     6481 actagaatgg taccgtgcat agtcactaag gcataataaa attcctgaga caatgcaccc
     6541 actcctgcat cattgataat gatccattta ccaaggatcg gtttcaacca ggacatatca
     6601 gactccggaa agcccaattg caatcggaag aaaacagaaa atgcagcccc gatgattgcc
     6661 cagatgatac cggtaattaa aaattgcctg gagataatct tatgatccaa gctaaaaatg
     6721 tacttgccaa tgaagccaga tttgtattta tcctctccat ggtgctcatg aaaatgatca
     6781 tcgtatccat ttttctcgat gatatcatcg acttcatcct tgacagttat attatgatca
     6841 gacatgtttt aaattttttc tatttctaaa attaaaaaat tattcagcct ttgtcgaatc
     6901 ggcttttgtt tcagatcctc cttccgctga tggagctggt ccattattta caacaccatt
     6961 gatagtaatt ggcttgcctt tattggggtc atcatccttg ccccttacgt ttgccaaata
     7021 ataggattgt tgcgacttta cccatgcatc gtactgttca ggagtcacca ccctaacagg
     7081 gcgtcgcatg gcaaagtgac tgtctccaca caattcggca caagccagtt cgaatgtaaa
     7141 tgcttcccat ttcaatttgc tctcaggatc acttggatca gcaggcacat tccattcagg
     7201 atattttttc agattattct tatactcttc agtggtcatg gaaggtgtga acacaaagta
     7261 ggtaggcatg cccggaacag catccatctt taccctgaag tgaggaagaa agaaactgtg
     7321 caatacatca cgggacaaaa ttctgattct tacttttttc cctttgggta aaactatttc
     7381 atcaggcttg aaatcatccc aatttttggg atcattccaa tcctgaccaa gatcattggt
     7441 tccggaagtt aatttaaaat cccttgtccc taatatatta tcaatacccg gatacctaac
     7501 aatccatgca aattgcattc ctgtaacctc gacctctata tgcttttcat ccggcttaat
     7561 atctgccatc atcttattcc acaccattaa accatcaatg gctaaaaatg cgagtacaaa
     7621 gaagggggtc aaagtccaca ccatttcgag cttagtgtta tcgggataat accaagcctt
     7681 tttacctttc ctatatcgat atctgtaagc aaagtaaaat aacaggatat gggtaatgat
     7741 atatacaatc ccggttagga tcaacatgat atgcatggat ttatcaatga gtgttccatg
     7801 ctcagaagca gaaagcattg gtccataacc aaacatcatg ttcttataat gatacgatgt
     7861 gtagatcgta aggaatagca acaagaaacc gaataccaat attgcaactg ctgtattgtt
     7921 attggattgg aggaaagctt tctcctcacc tttaattctc ttaataatct cattgattct
     7981 cgctatctga tagataacaa gcccaacaag caaaatacat ccaattattg ctaacgctgt
     8041 cattgattat ttaatgttaa attattgtca aaaagtttat tgaacatgat gatgtaaact
     8101 ctcaggcagc agaggatcat gcttgggttt aagcgacaat ttacccaact gtacaaaagt
     8161 tacataaaca aatagtccaa agaatcctaa catagttccg atctccaaca aacccggaag
     8221 agtaaagccg gagacaaaat tgcttgcatg ttcggcaccc tcagcaacag ctccatggcc
     8281 gccctccatt ttggcaagag cttcatgagc agtatgcaga gttcccggct taatcatcag
     8341 gaagaaatcc caccaatgac caaatataac cagtatacaa acaaacacca tcgatcctat
     8401 tttccgcttt gtatcatttc ttaataacac caggaaagga agtacaaagt tgatgacaag
     8461 gttaccccag aaaagtacgg gatattgttg catctgcctg ttgaaataag tcgtttcctc
     8521 tccattgtta ccgtaccaga tcaacatgaa ttggctgaac cacaaataag tccagaaaac
     8581 agtaattgca aaaacaaact ttcccacatc gtgcagatgt tctaccgtaa caaaattgat
     8641 aaaaccttgt ccctttagga aaatgatcaa caaggctgta aacgcaaagc atgctactac
     8701 ccatgaagct gtagcgtacc atgcaaataa ggttgaatac caatgtgcgt ccaatgacat
     8761 aatccattgc caaatcattg cagcacttga aaatcctgcg atcggcagaa atatactgga
     8821 tagcactttc atgcgcttat attccttgat atcatcaaag ccattattgt tatcctgctc
     8881 tttggacagt tgtctgagct tgattgcaaa ataagcccat gcaccaataa caataacagt
     8941 ccccaaagta taccaatatg gattaagaaa tccggattta cctttgagga tcaaatcatt
     9001 ggcaacatct tctgcatcgg tccaatgata aagatggtga taatgacccc atacgcctcc
     9061 taccacaaca agcatcaata tcaagccggt aatcaggaac atcgaataag cttcccataa
     9121 ccgcttgaaa gctacccacc aactaccata tgtaagaata cccatggtca taagcatcag
     9181 agcagcaaag gcaattccgg taaaatacat tgtattatgc aaatagttag accagaatct
     9241 ggtatgcaga gcatcatcct gaaaccatgt aataaccatg cttatcagtc caatgaccat
     9301 aaaggcaatc agtgtatttt tgtattttga tttgaatatg aattgatcca tcctcaatta
     9361 attttttgtt atcctcgaat ataacttatt actgttttct tttcactaat tggcaacagt
     9421 gggcatagta tcagctaccg caggcatcgc tttctttgct gaagctataa aatccgtatt
     9481 gttcaacgtg ttttctgtct catcatattt caggcctttt gtctttgcct gcaaagatct
     9541 gatgtattga atcacctgcc atctttcctt gtaagacaat ttatcggaat aagaacccat
     9601 cacattttta ccatgcatga tagcaaaata aaatcttcca ttcgatgcag caatgaattc
     9661 atcactggtt aggattgcag gttgcgcagg atacttacca tcatctctta caagatatcc
     9721 ggagccatcc cctttttcac catgacaaat tccgcagtag atatcataca actctttacc
     9781 atgagcaaga ttttctttgg tgatgggata aggattcaat ttgatatcat tttcggcccg
     9841 cttcctatct tcctctgtat caccataata gaaaggaact gaaccattga cggtttcatt
     9901 ggcaaattca gcttcaacag atgcagggat ctcactatta ctgtatttgg caagcccaac
     9961 aaatcctctt ggaattgttc ccatcacagg atttcgaggc tgagccaatt tgatatagcc
    10021 attcatgtcc caccgattat agtggaacaa atcaaccata ttagcctcat aggcgatgga
    10081 atggcccata tcctgcatat attcatggcc tggtttgtta cctcctgcag gagcacaact
    10141 ttcaagcgac aagctagctg caatgaatgc cagtccggaa aaaatcaaat atctattata
    10201 tttcatcttc aaaagaatga ttctttttat taaataaact tcgtgtcaac caaagaggca
    10261 ccatgctttg agaagaaagc tcgtaggccg gatttctgtt catcggatgc atcacttgta
    10321 tcaaatgaga tacagaattt atcatctgta attctgtcat gtaatgtctt tgccttgact
    10381 ccagggccca ttccgcagat tgtataaaaa gtcactacca ttcctataga ggcaaacaac
    10441 actgtcagct caaacattat cggtacaaat gcagggaatg agaagtaagg cttacctccg
    10501 aagatggtcg gccaatctct tgtaaatatc catgaaattc caaaaaaagc tgtcgcagtc
    10561 cctgtcaatc catagaaaaa tccggcaata tgcagtcttg actcttccaa gcctaacaac
    10621 ggatccaatc catgcaccgg aaaaggagta tagacatcgt caatttccat atgagacttc
    10681 cgtgcatcag caacggctga caacagttgg gtttcatcgt cgaacaaccc gaacaatact
    10741 tctttatttt ttgatgccat ttatctatat tctttaacta ctaattaatg atgttcttcg
    10801 tgatcagaat gagctaccac aacctcattc ttttcattat atccgcgatt ctttcctctg
    10861 tattgatctc cagaagattt aaggattgat tttacttcag caatagccac aacaggagca
    10921 atacggacaa atatgagata taatgtaaag aacaaaccaa aagtccccat atatattcct
    10981 atttcgttcc aggatggata ataataactc caactggatg gtaaataatc tcttgccagt
    11041 gttgttacta taattacaaa tcgctcaaac cacatcccga tattcacaaa aattgacatg
    11101 aaaaacgtcc accaaagact ccttctgatt ttctttgacc aaaatacttg aggggaaaga
    11161 acattacaag ccatcatccc aaaatatgac caccaaaaag gcccggcggc gcgattggca
    11221 aaggcaaatt gctcatatac atactgggaa taccaagata taaataactc tgtaaggtag
    11281 gcaattccca ctacagtacc agtaagtagg attaccttat tcatagattc aatatgattg
    11341 attgtaatgt aatcttccag tttcaaagct tttcgggtca ctaacattaa tgtttgaacc
    11401 attgcaaagc ctgagaaaat tgcaccggca acaaaatagg gagggaaaat ggtagtgtgc
    11461 catcccggga taactgaagt ggcaaagtca aaggatacga tagtatgtac ggaaagtacc
    11521 aacggtgtag caagccctgc caatactaag gataaagact caaatctctg ccaatgcttt
    11581 gctgcacctg tccaaccaaa agacaagaaa ttgtacaatt tccttctaaa tccacttgct
    11641 ctgtctctga gcgttgcaaa gtcaggaacc agaccggtgt accaaaacaa aagcgaaact
    11701 gtaaaatatg tagagattgc aaaaacgtcc caaagaagtg gtgagttaaa attgacccat
    11761 aaaggacctc ttgtatttgg taagggaata aaataaaaca ataaccaaat acgccccacg
    11821 tgaataacag ggaacataaa cgcacaaatt accgcaaaga ttgtcattgc ctcagccgcc
    11881 ctatttacac cggtacgcca tttttgacgg aacagcaaaa gaattgctga aatcaaagtc
    11941 cctgcgtgac ctataccgat ccaccaaaca aagttagtaa tgtcgtaacc ccatccgatc
    12001 gttctgttta agttccaggc cccaacaccg tagtaaattt gccatcccac acagaaaaca
    12061 aatactgcca aaaaaaatac cgcaataata aatcctatca accattgcct ggttggtgct
    12121 ttttcggagg gagtgcatat atcttccgtt atttgatgat acgtcttgcc cccgaaaatt
    12181 aatggttctc ttgttggtga aacaactata gacatcttcg ttttattatt ctgttattta
    12241 taatattaaa atatatctct atatattatt ctaattatca acttaaatcc gcatttttat
    12301 tagtcacgcg cattttatac ccaatctttc cctttacgtt aatttcttcc aaaacaaagt
    12361 aagttgtagg ctcatcccat tctttattga gaataccatc cttcatattt tgatcgccaa
    12421 aggttatcgc tccggcagga caagcggtag cacatgccgg cttgatatca gtttccttca
    12481 aaggcctcat ctctgcttta gccctcaact tgccttcctg aatcctctgt acacaaagag
    12541 agcatttttc tataacacct cttgaccgca ctgtcacatc cggattcaag accattctgg
    12601 taaggttgtc ctcatagaac agggactcac catcaggcat tacatttaat tcattcctgc
    12661 caaataaatc cgctgtggta taatctgccc aattaaacct tctaaccttg aatggacagt
    12721 tgttagcaca ataacgcgta cccacacatc tgttataggc catctggttg agaccttcag
    12781 cactgtggtt ggtagcattg accggacaaa cgttttcaca tggtgcatta tcacaatgct
    12841 ggcacatcat tggctggtaa aatacattgg gagattcgac atcaccataa taatatcggt
    12901 cgattcgtaa ccaggtcatt tcatgctggc gatgaacttc cttcttaccc actatcggta
    12961 cattgttttc tgagatacag gcaacctgac aagcaccgca acctgtgcat gcattcaggt
    13021 cgatactcat cccccaatgg tggccttgct tatacaagtg gtcaaatcca ggatagaggg
    13081 tttcattatt cagtttggca gctgttgccc tgaactcctt caatttttcg accttttctg
    13141 caaaattgct aagatctgct gactttaaga tactccggtc ggttaatcct ccctgaaatc
    13201 cggaaccaat agtcatgata gctttttcat cgacattgat ttccttatta gacttatcct
    13261 tacctcttac tcccaaagtg tgatgcaact gaacagaagc gaaatgtttg tcctcgccac
    13321 catttggttc aaccttcaca ttatcattcc aatatctgag gttaccatca gcatctcgag
    13381 tcagcgtgtt gatgatataa ttaccaacac ccatggaact tgcattacca tgtgtacgtc
    13441 cgtacccaac aggcatggcg aatgtttcgg ccatctgacc aaattggcgt accacaggta
    13501 catcctttac cgcagtcttt cccgatgaga gatcaacgaa aagaccatct tttttcaggt
    13561 tttgatagta gttaaatttg ctatccccat cccatgacaa tggaatatgg attatacttg
    13621 tccataccgt cctcgtaaca gggtccggca tttcctgaag ccagggatta tttgcataag
    13681 caccatagcc tacgccaatc ggctcataat atttcacttc gaggcctgaa cccggcttgg
    13741 tgactgcgct acctagtcct gaaacatcaa cagcaacgga acctgcccca ctttgagaag
    13801 caagttcaat caatccatta tgcaaggtat tatcccaaaa tctctgaaaa tcagaatatt
    13861 cactctgtaa tccaaacagg gaggatctcc atactccctg catatatgta gagaaatcat
    13921 ctaccggggt cttggcccat gccagaagcg attgctctgc ttgccgtgta gcaaacaagg
    13981 gtgcaatcgt gggctgcata atataataat tattctttct tggctgagca tcgccccatg
    14041 actccaggaa gtggttggaa ggtgcaacat agccacaagc tgcactggtc tcatttggaa
    14101 ctgttgatag tgaaacggac aatccggttt tctttaaccc tgcagcaaaa tctttgccct
    14161 caggcagatc aaacacagga tttgcatcac caaggatgaa tactgcgctg acgcttccgg
    14221 cattcatttc ttttatcagc ccaacaatat cactatctat accctttctt tgattagagt
    14281 aattgttgag gtcaatggta cgaccgtagt tacccaacaa atcattgatc ttatttacaa
    14341 tgatttgctc cgcgatatta ttagaatcag agagtaccaa agaggccttt ccggcatttt
    14401 tattatccac caaattcttt gctactgcag caaaagattt ttcagccttt tgatttttta
    14461 aagtgccact gactgacagt tgactacctc ctgtcaaaga cgcaactgca ttgtatagct
    14521 tagcaatagc caatccctgt tcagatggtt tgatcaacag tctgttgtcg gcattggagc
    14581 cggtgtaaga catattactc tcatattgaa tcaaacggct gatttcaggt ttgtcgaagt
    14641 ttttgatttt tcgatttttg gtccaggcat ttgtgtattc tacaggagac acccaggttc
    14701 ccagaaagtc agctccaaag ttgaccacta tttgggcgtt accaaaatca tagcccggga
    14761 aaacacgttt accgaatgat cgttcatttg caacaagggt cgcggaggaa gaatatggat
    14821 cataggtgac atgcttggca gtaggatagg cagtaatgaa ctgatttatg actgcattca
    14881 tggaggggct tgtaatggaa ttggagacaa tcctgatgtt accaccggaa gccagtttgt
    14941 ccatgactgc tttgtcaagt tcggaccact tcattttctt aatacctccc ttgccatcgg
    15001 actttcctgc gaacttatat cttccggtat catagagatc aagtaccgat gcctgggccc
    15061 tggcacttgt accgccacct gagaaggaag acaatgtatt tccttcaatc ttgataggtc
    15121 tgccctctcg ggttttaaca actaccggca atacatcatt gcccttaata aaagtggatg
    15181 cataatagtt ggccacaccg ggtacgatac tatccggcat tttaacataa ggaagagcct
    15241 ttctaaccgg tgtttcacaa ctggcagcaa tggttgctgc gcctatgctg aatccgagat
    15301 atttcaggaa gtctcttctg ttatgacctt tggcagattc atcctcttcc tttgacatat
    15361 tattgagtat gggcaactca tagaactcat cgtgaaggcc tttgacgaaa gcctcatcat
    15421 tagtgaactc ttcgctgctt ccccaatatc cttgatcaaa ttcgctcata ttattatata
    15481 ctagaaagtt ctgattatta taatgaaatt aaattaatag tgacattttt gacattcaag
    15541 tcctccgacg tctgcaacgg tcaccttatc tcgcttgcca tccttcaatt cctgatgata
    15601 acggacatag ctgtcataat atggattctc attaaactgt actttagtat ccctgtgaca
    15661 gttgatacac catcccatgg aaagaggaga gtactgacgc aaggtctcca tttcggcaac
    15721 cttaccgtgg catgcttcac aatccacttt accaaccttt acgtgttgtg agtgattaaa
    15781 ataaacatga tcaggaaggt tgtgtaccct tacccattcg attggtccct gaatcttctt
    15841 cttatggtcg ttggtaaggg agcttacaat gtgatcccat tggttcttca caaattcctt
    15901 accttccgca tccatggaat gtccctcctc tgtcaggtac tggttgccaa tccatttttt
    15961 gtatattttg gcgatatcct tttgagacat cttttcataa ttttcgatat actgatcggt
    16021 attcggatcg aatccaatag aggcaaatat ctttgtaatt tcctgtgttc catatgtcga
    16081 acctttcttg atagccttgt gacagttcat acaggtgttg gtggaaggaa tcactgaatg
    16141 tttacttctt cgcgctccat catggcaaaa ctgacaatca atcttgttga tgcctgcatg
    16201 tgttacatgt gaaaatttaa tgggttgttc aggagcataa ttttgctggc gacccaggtt
    16261 aatcgcatta ttaacggtag taaagctacc cagaacgatc agggcaaaaa ggaccacacc
    16321 aattactgat ttactggtaa gaagctgaac aattgtttta agttccaccg gttgaccttc
    16381 cttttcccgt tctacgtttt taaggtt
//
LOCUS       DAOQQN010000014        15973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1151_length_15973_cov_5.601395, whole genome shotgun sequence.
ACCESSION   DAOQQN010000014 DAOQQN010000000
VERSION     DAOQQN010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 15973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15973
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1151_length_15973_cov_5.601395"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PLG24_01070"
     CDS             complement(<1..1010)
                     /locus_tag="PLG24_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HMZ23500.1"
                     /translation="MKRIVYFLVMIMTSLQPSMSFTQETVNDKMQWFRDAKLGIFIHW
                     GIYSVDGVDESWSFYNHKLPYEKYMEQLKGFTARNYKPADWVKLIKETGARYTVLTTK
                     HHDGVALWDTKANDLSVVKLTPSGRDVLTPFTKEVRKAGLKLGLYFSILDWSHPDYPG
                     FLKDSNRYAIKDDSVRWNRYLRFYQKQIKELSNDYNPDLFWFDGDWEHSADEWQADKV
                     RANILEHNPRTIINSRLTGRGDYDTPEQNFPVTRPERVWELCMTCNNNWGYHPDDTNY
                     KTPYEVITIFADAISNGGNLLLDIGPREDGWIPDEQVHILKELGKWNKKHAEAIFGTV
                     AG"
     gene            1206..1706
                     /locus_tag="PLG24_01075"
     CDS             1206..1706
                     /locus_tag="PLG24_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23501.1"
                     /translation="MDEKILFKSFLSFFAEADFPLILSYDIDEPESFSDDPIPEGLAR
                     IFIAEEEGEDAFNEYVPVVQFRLNKNYLACIVWKASLQGYEFILIIYDNEGNIQESDS
                     VAGTYHIHEPMIYKIAEFASPNRAVVVEGNIDTRNKDANLPPARKFILNISDIGEINY
                     SLVGNL"
     gene            1708..3870
                     /gene="rnr"
                     /locus_tag="PLG24_01080"
     CDS             1708..3870
                     /gene="rnr"
                     /locus_tag="PLG24_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HMZ23502.1"
                     /translation="MTKKYPKSFKGRKLNTLELKIIVNRELKNLNGKSYSAAGLIKKL
                     KISNNRDEVQLILEKLVKEGRVRQTTGGVYQSNNKSSIPEKLFIGKVDLIRSGAAYII
                     VEGKSDDIYIAPGDLMGALDGDTVEVSVVRRKGRQRSDGRVIRIIRRSREIFMGRIFV
                     NKKYSVVTPFKTPDGFEILVFNEGLNGAEDGDIVTVRVISWPDGKNKMIKGEITEVLS
                     ADNASDMEMKSILIGQGFDLHFSKETLKEAHAIPDSIPEQEIMQRRDMRDIATFTIDP
                     ATAKDFDDALSYFLLEDGNMEIGVHIADVSHYIKPGGPMDKEAYERSTSVYLVDRVLP
                     MLPEKLSNELCSLRPNEDKLTFSAVFVFDKDTKVVSRWFGKTIIHSDRRFTYEEAQEI
                     IESGVGEYVEELMQLNTIAKKLRDIRYAKGAISFESDEVQFRLDENGVPVEIFIKERK
                     DAHLMVEEFMLLANRSVAEYIHQKGASGEIPFIYRVHDRPDNQKLSEFATFAAELGFK
                     MDLSKPAAVIHSFNLLAKKAEEDETLRLITPLAIRTMAKAEYSTNNIGHFGLGFDDYT
                     HFTSPIRRYSDVLAHRILFRNLNSAIYREDKSLLDVFCKHISAQERKAMEAERESVKY
                     KQVEFMSRFVGTEFNGIISGMHDRGVFVELDDNHCEGMIRFDRFDESFILDDSKMKAF
                     GRISGRTIKIGDRIRIKVLNADLDNRQLDFAPVMNQEA"
     gene            5132..5563
                     /locus_tag="PLG24_01085"
     CDS             5132..5563
                     /locus_tag="PLG24_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ23503.1"
                     /translation="MSACAGGSDSSNATEAATEPKTFDTESYDPKRGEGKFNAENVKV
                     EGLDAAMAAKGKSISETKCFSCHKTTDERLVGPGWKGVTAKHSPFWIMNFITNPDPMI
                     DKDPELQAQLEICLVRMPNQNLNDQEAREILEFMRENDGAK"
     gene            5767..7740
                     /gene="nosZ"
                     /locus_tag="PLG24_01090"
     CDS             5767..7740
                     /gene="nosZ"
                     /locus_tag="PLG24_01090"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HMZ23504.1"
                     /translation="MSSIKLSIVSTLFLALMVVSCKPKGTTNAISGNAAQKTYVAPGK
                     YDEFYNFVSGGFSGQLSVYGLPSGRLLRVIPVFSVDPEKGWGYSEETKPMLNTSHGFV
                     PWDDLHHVEMSMTDGVADGKWVFVNGNNTPRLARIDLKTFRTAEIIELPNSAGNHSSP
                     FGTENSEYIVAGTRFSVPGDYEPGDVPIESYKQNFKGHISFVSVDKNSGEMNIAFQLR
                     TPGVNFDLARCGKGKSHGWFFFSTYNTEQANTLLEVNASKNDKDFIMAVNWKKAEEYI
                     KAGKGKKQSVKYAHNKFNEETHSATSVIKNEVTILDVAEFPDIVYFIPCPKSPHGCDV
                     DPTGEYIVGSGKLAALLPVFSFDKIQKAIADKAFEEQKYNGIPVIKYEAALYGEVQKP
                     GLGPLHTEFDANGNAYTSFFVSSEVVKWNIKDLKVLDRQPTFYSVGHLMVPGGNTKKP
                     FGKYLVAYNKITKDRYLPTGPELSQSAQLYDISGDKMELILDYPTIGEPHYAQAIPGE
                     LIKDKQVKIFDIKANKHKYAAHGEAETKVVREGNQVHVYITAIRSHFAPDCIEGVKVG
                     DEVYFHVTNLEQDWDVPHGFGVKGANNAELLIMPGETATLKWTADRVGIFPIYCTDFC
                     SALHQEMQGYVRVSPKGSNTPLKFWLNNKADVQ"
     gene            8100..8678
                     /locus_tag="PLG24_01095"
     CDS             8100..8678
                     /locus_tag="PLG24_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23505.1"
                     /translation="MKNRNSNFSRVILFVSSLLLVCVLFVPLWRIDLDAPQYPEGLRL
                     LLYPHKLGGDVDIINGLNHYIGMKTLHTEDFIEFKVLPYLIGFFALLFLVSGIINTRK
                     WLWISVISFVIFGIVSMIDFYRWEYDYGHNLDPNAAIKVPGMAYQPPLIGFKQLLNFG
                     AYSIPSTGGWLFVGAGVLLVLALVKEKYMKKS"
     gene            8675..9118
                     /locus_tag="PLG24_01100"
     CDS             8675..9118
                     /locus_tag="PLG24_01100"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HMZ23506.1"
                     /translation="MKNILFVIICLAFVTGFNSCSTDGPEPILLNKDMCDNCRMNISD
                     ARFGAEVQTQKGRYYKFDDLKCLAHYAVEQPNGPIKSFYIHDFSQNNVLIEATKAYYV
                     WSKEFSSPMRGNTAAFANRADAEKVAQQYGSAVDDWETAKKRILE"
     gene            9140..10399
                     /locus_tag="PLG24_01105"
     CDS             9140..10399
                     /locus_tag="PLG24_01105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HMZ23507.1"
                     /translation="MSGFYNKAAINILLFVLLVLVSDSVSGSVLEVGKGKRYATINDA
                     LKQARDHDTVVIFSGLYKEGNIRITRPLSIIGRDWPVLDGLKKTEIITVRASNVNISG
                     LVLKDCAQGSMTDPAAIRVAEASNVNIIGNRLQNNFFGIYLQYAKHCLIKDNQIFASQ
                     KHEYQSGNGIHCWKSDSLQVIGNKIAGHRDGIYFEFVTNSIIWRNIAENNLRYGLHFM
                     FSNDDAYITNFFRNNGAGVAVMFTKRVTMINNTFTENWGDAAYGLLLKEITDCYMSGN
                     KFFKNTTGIFLDGSNRITIEKNSFNGNGWGMRMQANCMDNYIARNNFMGNTFDVATNG
                     TLTLNKFEENYWDNYDGYDLNKDKIGDVPYRPLSLYSVLVEKNPPAMLLYRSFMITLL
                     DRSERIIPSLTPENFIDNAPKTVPYQI"
     gene            10396..11109
                     /locus_tag="PLG24_01110"
     CDS             10396..11109
                     /locus_tag="PLG24_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ23508.1"
                     /translation="MIEIKNVSKKFGKLQALDKLNLTLQQGECVALIGPNGCGKTTLI
                     KSILGMVLPDEGEIIFNGKSIKHDINYRNEIGYMPQIGRYPENMTIGQVLELIKGIRD
                     GHQETDKELYEQFLIATMLEKKMRTLSGGTTQKVSAVLAFLFRPKVLILDEPTAGLDP
                     IAALILKDKILKAKEQGCLILITSHLLNELEDIVSEILFMQEGKLILHDSVDRLKELT
                     GEDSIARSIVHLLKENGHV"
     gene            11102..11869
                     /locus_tag="PLG24_01115"
     CDS             11102..11869
                     /locus_tag="PLG24_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMZ23509.1"
                     /translation="MFKVLKYVALDIFRNKIIIAYTLMLALFSWSAFALEDNVAKGLL
                     TELNIILLTVPLFSVLFSTIYVYNSSEFIELLVSHPVKRNVIWTALFVGLSLSMVGAF
                     IISTGIPIIIFADFATGLMMIVAGSVLSVIFVAIAFLSSIMARDKAKGIGISIILWLY
                     FALLFDGLVLFLFLQFSDYPIEKFVVMLSALSPIDLCRILVLLRLDVSAMMGMTGAVF
                     KDFFGTGGGLAVSFLLLLMWIAVPYGISLRKFKHRDL"
     gene            11912..12277
                     /locus_tag="PLG24_01120"
     CDS             11912..12277
                     /locus_tag="PLG24_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HMZ23510.1"
                     /translation="MKTDITNKQDILKIILIFYDRVKTDDVIGIFFTEVVQINWDKHI
                     PHMVSFWESVLFYTGDYSGSPINTHRGIHQKYKTQPKHFARWIKLFNQVIDENYSGPN
                     AEKMKNHTISIAKVMQQKI"
     gene            13007..13642
                     /locus_tag="PLG24_01125"
     CDS             13007..13642
                     /locus_tag="PLG24_01125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HMZ23511.1"
                     /translation="MDIVSKAIEDYCESFGDNPPGLLLDLDTYTHEHHHAPNMLSGSF
                     QGRVLAMISRVVRPKTVLEVGTYTGYSALCLAEGLADGGVVHTIDIDERLRQTHELFI
                     GQSRYADRIKVYFGDAKILIPGLGLRPDLVFIDGAKKDYSAIFDMVLPMMNSGGVILA
                     DNVLWKGKVLDEEMDDRTAAIHEFNRKVSQCELVYKVLLPVRDGLFWITKK"
     gene            13644..14522
                     /locus_tag="PLG24_01130"
     CDS             13644..14522
                     /locus_tag="PLG24_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23512.1"
                     /translation="MEFLRRKDIDTDRWDAVIDSSATGLPYAYSAVLDECCNGKWDAI
                     VGDAYNYVLPLPFNRKLFGFKQYYQPYLLQQLGVFGNMPASVEMDAIMHMIKSHSIRS
                     MIAFQEENTQLLAGAGGNLKTNFVLDLLPGYDYLSSQYKKNLKQRIGRSFDDEFTGSE
                     GLSMDDAIDMYFQWTYAKYNKSEKLSKRYIHNWVRIFNHLGYAHVVALRAQDATIHSG
                     IIYLKTHSRIILSLQFSHPDYKQYSGPSRLIDYLIRQHAGSRMRLDFEGSQIPSIAEF
                     YGSFTPQNMPYTVIKS"
     gene            complement(14519..>15973)
                     /locus_tag="PLG24_01135"
     CDS             complement(14519..>15973)
                     /locus_tag="PLG24_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HMZ23513.1"
                     /translation="EANVKESTGLFTIFLLGFIGGLIALLTPCVFPMIPLTVSFFTKS
                     NKDKKKGITNAFLYGFFIFLIYILLSVPFHLLDSINPNILNDISTNAWLNVAFFLIFM
                     VFAFSFFGYYEITLPSSLSNKVSSAEGVGGVIGIFFMALTLALVSFSCTGPILGSLLA
                     GTLSSNGGAWNLTAGMGGFGIALALPFALFAAFPSWMNTIPKSGGWLNTVKVVLGFIE
                     VALAFKFLSNADLVKHWGLLKIEPFLLIWILVALGLFAYLMGWIRFPHDSKLKKLSPT
                     RVVLALISLVFAIYLATGFIYNKETKTFTSLSLLSGLAPPVGYSILYPNDCPNNLNCF
                     HDLEEGLAYAKSRNLPVFLDFTGYACVNCRKMEEHVWPTNKVKNLLTNEFVVISLYVD
                     DKTPLPEDQQVTLTYKNGGTKRIKTVGDKWNFFQTDNFNNNSQPLYALITNTGKLLNT
                     PVGYTPDPEQYATFLQCGIDAFKASEGKETIGSR"
BASE COUNT         4726 a         3123 c         3419 g         4705 t
ORIGIN      
        1 aaccctgcca cagtcccaaa gatcgcctcc gcgtgctttt tattccactt gcccagttcc
       61 ttcaggatat gcacttgctc atcaggtatc cagccatctt cccgtggacc aatgtccaac
      121 aaaagattac cgccattgga tatggcatcg gcaaatatag tgatgacttc atagggtgtt
      181 ttgtagttgg tatcatccgg atgatatccc caattgttat tgcaggtcat gcataactcc
      241 cagacccgct caggtctggt gacagggaag ttttgctcag gagtatcgta atcaccacgg
      301 cctgttagcc ttgagttgat gatggtccgg ggattatgct ccaaaatatt tgcccttacc
      361 ttatctgctt gccattcgtc agcactatgc tcccaatcac catcaaacca aaacaaatcc
      421 ggattgtaat cattactcaa ctccttaatt tgcttctgat agaaccttag ataccgattc
      481 catcgcacac tgtcatcctt gatcgcatat ctattactat ctttcagaaa acccggatag
      541 tcagggtgac tccagtctaa gattgaaaaa tataacccca gtttcagccc ggcttttctc
      601 acttcctttg taaatggagt caggacatcc ctgcctgatg gagtgagctt gactacacta
      661 agatcgttgg cttttgtatc ccacaaggca actccatcat gatgcttggt tgtgaggaca
      721 gtataacgcg caccggtttc cttgatcagc tttacccaat cagccggttt atagttccgt
      781 gcagtaaacc ccttgagctg ctccatatat ttttcataag gcagtttgtg attataaaac
      841 gaccaactct catccacacc atcgacagaa taaattcccc aatgaatgaa gatacctaat
      901 ttagcatcgc gaaaccattg cattttatca tttacagttt cttgagtaaa ggacatggaa
      961 ggctgaaggc tggtcataat catgactaaa aaataaacta ttcttttcat gaataagtca
     1021 aacattactt gggagttaat aattcggcta aaataactat ttttccttac aggaaataat
     1081 ttagtcaatt attacctata tatggtcagg aatgtcaaat aatcaatttt ttcgcaactt
     1141 ttgccaatgg aaatttgttc ctcctcaatg atttaggtgt aaaacttaaa attctacttt
     1201 ctaccatgga cgaaaaaata cttttcaaat catttctttc tttttttgcc gaagccgatt
     1261 ttcctttgat tttatcttac gatatagatg aaccggaatc attttctgat gatccgatac
     1321 ctgaaggact agcgagaatc ttcatcgcgg aggaggaagg cgaggacgca ttcaatgaat
     1381 atgttcctgt tgtgcagttt cgactgaata agaattatct ggcgtgtatt gtatggaaag
     1441 caagcttaca aggctatgag tttattctaa ttatatatga taatgaaggc aatattcagg
     1501 aatccgattc tgtggctggc acctatcaca tccatgaacc tatgatatac aaaattgctg
     1561 aatttgcttc tcccaaccgc gctgtagtag tcgaaggcaa tattgatacc agaaataagg
     1621 atgccaattt gcctccggca agaaaattta tcttaaatat tagtgacatc ggagaaatca
     1681 attactccct tgttggaaat ctgtaatatg acaaaaaaat atcccaagtc cttcaaagga
     1741 aggaagttga atacactgga actaaagatc atcgttaatc gtgaattgaa aaatctcaat
     1801 ggcaaatcat actctgctgc gggcttgata aaaaaactta aaatcagtaa caaccgcgat
     1861 gaagtccaac taattttgga aaaactggta aaggaaggca gagttagaca gacaaccgga
     1921 ggtgtttatc aaagtaataa caaatcatcc attcctgaaa aactttttat tggaaaggtg
     1981 gatttgattc gttctggagc cgcctatata atagtagaag gaaaatccga tgatatctac
     2041 atagctccgg gagacctgat gggcgcactt gatggcgata cagttgaggt atcagtggta
     2101 agaaggaaag gccgacaaag atccgacggc agagttatac gtatcatacg ccgctcaagg
     2161 gaaatattca tgggccgaat tttcgtcaat aaaaaatata gtgttgtcac ccctttcaag
     2221 acacctgatg gttttgagat attagtcttc aatgaaggat taaatggggc ggaagatggt
     2281 gatattgtga ctgttcgtgt cattagctgg cctgatggta aaaacaaaat gattaagggg
     2341 gaaatcacgg aagtgttaag tgctgacaac gcctccgata tggagatgaa atctattttg
     2401 ataggccaag gctttgatct gcatttttca aaagaaacgc tgaaagaggc tcacgccata
     2461 ccagacagca tcccggaaca agaaataatg cagcgcagag atatgcgcga tattgcgaca
     2521 tttactatag accctgctac agcgaaagat tttgacgatg ctctttcata ttttttactg
     2581 gaggatggta acatggagat aggagtacac atcgctgatg tttctcatta tatcaagccg
     2641 ggtggcccga tggataagga agcatacgaa cgcagcacat ccgtatatct tgtcgatcgt
     2701 gttttgccga tgctgccaga aaagttatcc aatgaattat gttccctgcg acccaatgaa
     2761 gacaaactga cattttctgc tgtatttgtt tttgacaagg atacaaaggt tgtttcccga
     2821 tggtttggta agacaattat acatagtgac aggcggttta cttatgaaga ggctcaggaa
     2881 atcatagaat ccggtgttgg ggaatatgta gaggagttga tgcaacttaa cacaatcgcc
     2941 aaaaagctac gtgatatacg atatgccaaa ggagccatat cgttcgagtc ggatgaagtt
     3001 caattcagat tggatgaaaa tggagttccg gtggagatct ttattaagga aagaaaggat
     3061 gcccatctga tggttgaaga gtttatgttg cttgccaatc gatctgtagc tgagtacatc
     3121 catcaaaagg gagcaagtgg cgaaattcca tttatttacc gcgtacatga ccgcccggat
     3181 aatcagaaat tgagtgaatt tgccacattt gctgcggaat tgggattcaa aatggacctt
     3241 tccaaacctg cagccgtcat ccattcattt aatctcctgg ctaaaaaagc tgaggaggat
     3301 gaaacgctga gactgattac acctcttgcc atacgcacaa tggcgaaagc cgaatattca
     3361 accaacaata ttggccattt cggtcttggc tttgatgatt acacccactt tacttctcca
     3421 attcgtagat atagtgatgt cctggcacac cgaatcctgt ttcggaatct gaatagcgcc
     3481 atttatagag aggacaaatc gctgctggat gtattttgta agcatatttc agctcaggaa
     3541 cgaaaggcga tggaagcgga acgtgaatcc gttaaataca agcaggttga gtttatgagt
     3601 cgatttgtcg gtactgaatt caatggaatc atttccggaa tgcacgaccg tggtgtgttc
     3661 gtggaactag atgacaatca ttgtgagggg atgatacgat tcgaccggtt tgatgaatct
     3721 tttattctgg acgacagtaa aatgaaggca tttggtagga tttcaggccg aaccatcaag
     3781 attggtgatc ggatcagaat taaggtgctt aatgctgatc ttgacaacag acagttggat
     3841 ttcgcgcctg taatgaacca ggaggcttaa aaatagacat caaccaggga gggtgcatcg
     3901 ccgtaggtta tactttatca aagatttgaa tggtccaaga gtaaattttt aaagattaat
     3961 tcagatggtc aacggatagc aacagagatt gacaggttac cattcctcga tatcggagga
     4021 ttggaggaaa gacatcaggg taaaggtatt ttgtggtttc ccaaagtagg tagatgatta
     4081 ataattacct tgtcaataga attgttcttc tccaggtacc tttatgtaaa ttctattcaa
     4141 gattgagcat attttttaat cgtgccgggt gtatggtgca ttatacctca gtcgacggtt
     4201 attaaacatg tacatacttc attttttttc caaaaaataa ccaaacaccc actactgcaa
     4261 cactcacctg aagtaggtag atgattaata gtcaccgtgt caataaaatt gctcttttcc
     4321 aggtgccttt aagtaaattc tattcaagat tgagcatatt ttttaatcgt gccgggtgta
     4381 tggcgcatga tacctcagtc gacggttatt aaacatgtac atacttcatt tttttcccca
     4441 aatgtaacca atcacctact gcaacactca cctgaagtag gtagatgatt aatagtcacc
     4501 gtgtcaataa aattgctctt ttccaggtgc ctttaagtaa attctattca agattgagca
     4561 tattttttaa tcgtgccggg tgtatggcgc atgatacctc agtcgacggt tattaaacat
     4621 gtacatactt catttttgct tttctgccta atctcatttt ctatttttct ttttgaagct
     4681 atccggccaa tccccgttta attatcttta attcgtaaat tttttggaca aagccgtcag
     4741 ttattccctg tttccaacac aaggactaat ggactcagga agaaataatt cctgaaaaat
     4801 attgcgcctt gtaaaaaaaa tattgacgga gtttgaaaaa accccaaaaa aatcaaaccc
     4861 aaaatatgtt ggacggtatg gcctttaggg ttatattatc ttgtttttca caaattagca
     4921 caatataatt gcatcttttg cctaaatgat aaaatattta caagtaagtt ttttttgact
     4981 gtaaatctct gcagttacta atttttcatt tatgactgtg gtcataaaaa ttgacccaaa
     5041 agaagagtca attttgacaa aaatttaaaa cttgttaaag atgtcaaaac tttttattgc
     5101 gatttccaac ctgctcatta ttgtgatgtt tatgagcgct tgcgcaggtg gatccgacag
     5161 cagcaatgca acagaagctg ctactgaacc caagactttc gataccgaga gttatgaccc
     5221 aaaaagaggg gaaggtaagt tcaacgcaga gaatgtgaaa gttgaaggtc ttgatgcagc
     5281 aatggccgcc aagggaaaat ccatttccga aactaaatgc ttttcttgtc ataagactac
     5341 tgatgagcgt ttggtgggac caggatggaa gggagttaca gccaagcatt cgccgttctg
     5401 gatcatgaac tttatcacaa atcctgatcc gatgattgac aaagaccctg aacttcaggc
     5461 acaattagag atctgtctgg ttaggatgcc gaaccaaaat ctcaatgacc aggaggcaag
     5521 ggagattctt gagtttatgc gtgaaaacga cggcgcaaaa taagttaagg agccggtatt
     5581 gataccggtg aattcatata gatttattcc atatttcggc ttttaatttg ccgtatccgg
     5641 gtggtagcac aactcgaaat gggaatgcat tgtgcccaaa gtatgggttt tatgaatctt
     5701 ttcaattatg tttagaacgg tatcaatttc ccgataatat ttcaaaactc attaatattt
     5761 tttaatatgt ctagtatcaa attgtcaatt gtatcaacac ttttccttgc gttgatggtt
     5821 gtttcttgta aaccaaaagg aactaccaat gctattagtg gaaatgctgc acaaaaaact
     5881 tatgtggcgc ccggtaagta cgacgaattt tataacttcg tatccggtgg attcagcggc
     5941 cagctgagtg tttacggcct tcctagtggt aggcttttaa gagttatccc cgttttttca
     6001 gttgaccctg aaaaaggttg gggttacagt gaagagacca agccaatgct taacacttct
     6061 catggcttcg tgccttggga tgacctgcac cacgttgaaa tgtccatgac agacggagtt
     6121 gcagacggaa agtgggtttt tgtcaacgga aataatacac cgcgacttgc aagaattgac
     6181 cttaaaacgt tccgtactgc cgaaatcatc gagctgccaa acagtgccgg taaccacagt
     6241 tctccatttg ggaccgagaa ctctgagtat atcgttgctg ggacacgatt cagtgtacca
     6301 ggtgactatg aaccaggtga tgttcctatt gagtcttaca agcaaaattt caagggacac
     6361 atttcctttg taagcgtaga taagaattcc ggtgagatga atattgcatt ccagttgagg
     6421 actccgggtg ttaactttga cctggcccgc tgtggaaaag gtaaatctca tggatggttc
     6481 ttcttcagca cctataacac agagcaggcc aatacactgt tagaagtgaa tgcctctaaa
     6541 aatgataagg actttatcat ggccgttaac tggaagaagg cagaagaata catcaaggcc
     6601 ggaaaaggta aaaaacagtc tgttaagtat gctcataaca agtttaacga agagactcac
     6661 tcagctactt cagtaattaa gaatgaagtt actattctgg acgtagccga attccctgat
     6721 atcgtttatt tcattccttg tcctaagtct ccgcacggtt gtgatgttga cccgacaggt
     6781 gaatatatcg taggttccgg aaaattggct gcattgttac ctgtattcag cttcgataag
     6841 attcagaaag ctatagctga taaagctttc gaagagcaga agtataatgg tattcctgtt
     6901 atcaaatacg aagcagcact gtacggcgag gtacaaaagc cgggattggg cccacttcat
     6961 acggagtttg atgccaatgg aaatgcttat acttccttct tcgtctcttc tgaagttgta
     7021 aaatggaata tcaaagatct taaggttctt gaccgccagc ctacattcta ctcagtagga
     7081 cacttgatgg tacccggtgg taatactaag aaaccatttg gtaaatattt ggtagcctat
     7141 aacaagatta caaaagaccg ttatcttcct acaggccctg agttgtcaca aagtgcccag
     7201 ttgtacgata tcagtggcga caaaatggag ctgattcttg actacccaac catcggagag
     7261 cctcactacg cacaggccat acccggggaa cttatcaagg acaaacaagt taaaatcttt
     7321 gacataaaag ctaataagca caaatatgct gctcatggag aagctgaaac aaaggtggtt
     7381 cgtgaaggta atcaggtaca tgtatacatt acagctatta ggtcacactt cgcacctgac
     7441 tgtattgaag gcgtgaaagt tggcgatgaa gtgtacttcc atgtaaccaa ccttgaacaa
     7501 gactgggatg taccgcatgg tttcggagtg aagggagcta acaacgcaga actcttgatt
     7561 atgccgggcg aaacagctac cttgaagtgg acagctgaca gagtgggtat attcccaatc
     7621 tattgtacag acttttgttc agctttgcac caagagatgc aagggtacgt tagggtttct
     7681 cctaaaggat ccaatacacc tttgaagttc tggttgaaca acaaagcgga cgtccaataa
     7741 attttcattt ctaaataaat ttcataaggg gcatttcgtc cgcaaatgat gatttgcccc
     7801 tttacctttg aagcttgagt ctcctctgat tttgaatatt tctatcttcc tggcgaaaat
     7861 acttctactt tgtaggtagg tcggtatttt cacacttaag ccaataaaga ttgactgagc
     7921 gatgattata tttttccctg agaattcggg cttcatgatt ttaattaggt tctgaaacct
     7981 gtttttatgg aggacatgtg ggtggtgata tatccttatg gaatcctgaa cggggccgcc
     8041 tcaaggaagt agaatagccc ttcgccatgt atttatcttt taaccaactt atactcacta
     8101 tgaaaaatag aaattccaat ttctccagag taattctttt tgtttcttcc ttactactcg
     8161 tttgcgtttt gttcgttcct ctatggagaa ttgatctgga tgctccgcag tatccggaag
     8221 gcttgagatt gcttttatat ccacacaaac tcggaggtga tgtagatatc atcaatggtc
     8281 ttaatcacta tataggcatg aaaacccttc ataccgagga ctttattgaa ttcaaggttt
     8341 tgccttattt gattggcttt tttgccttgt tgtttctggt ctctggaatc attaatacaa
     8401 ggaagtggtt gtggatttcg gtgataagct tcgtaatctt cggtatagtt tcgatgattg
     8461 atttttatag atgggaatat gattacggcc acaatctgga tccaaatgcg gctatcaagg
     8521 tgccgggaat ggcctaccaa ccacctttga tagggtttaa gcaattgctc aacttcggag
     8581 catattccat tccttccacc ggaggttggt tgtttgttgg agcaggagtt ttactcgtac
     8641 ttgctcttgt taaggaaaag tatatgaaaa aatcatgaaa aatatattat tcgtaatcat
     8701 ttgcctggct tttgttactg ggttcaattc atgcagtacc gatggccctg agcctattct
     8761 tcttaataag gatatgtgcg acaattgtcg tatgaatatt tccgacgcaa gattcggagc
     8821 ggaagttcag actcagaaag gaagatatta caaatttgat gacctgaaat gcttggcaca
     8881 ttatgcagtc gagcaaccta acggtcctat aaaatcgttc tatattcacg atttttcaca
     8941 aaataatgtt ttaattgaag ccaccaaggc gtattacgtt tggagcaaag agttttcaag
     9001 ccccatgaga ggcaacacag cagcttttgc caatagagcc gatgctgaaa aggtagctca
     9061 gcaatatgga agtgctgtgg atgattggga aaccgctaag aagcggatcc tggagtaatt
     9121 gttattaact attttttgaa tgtccggctt ttataataag gcagcaataa atattttgct
     9181 ttttgtccta ttggtgttgg tttcagattc ggtgtccggc tctgttctgg aagtaggcaa
     9241 aggtaaaagg tatgccacaa tcaacgatgc gctgaagcag gctcgggatc atgatactgt
     9301 ggtaattttt tctggattgt acaaggaggg aaatattaga attacgcgcc ctctttccat
     9361 catcggtcgt gactggcctg ttctcgatgg cctcaaaaag accgaaatta ttactgtcag
     9421 agcttccaat gtcaatattt ccggattggt tctgaaagat tgcgctcagg gctctatgac
     9481 cgatcccgct gcaatcaggg ttgctgaagc aagcaacgtt aatatcattg gcaatagact
     9541 tcaaaataat ttttttggca tttatcttca atacgccaaa cattgcttaa tcaaggataa
     9601 tcaaatattt gcctctcaaa aacacgagta ccagtcaggc aatggcatcc attgctggaa
     9661 aagtgatagc ctgcaagtaa ttggtaataa gattgccgga catcgcgatg gtatctactt
     9721 tgaattcgtt accaattcaa ttatctggag aaatattgct gaaaacaatc tcagatatgg
     9781 gcttcatttt atgttttcaa atgatgatgc ttatattacg aatttcttca ggaataatgg
     9841 agcaggagtg gccgtcatgt ttaccaaaag ggtaaccatg ataaataata ctttcacgga
     9901 aaattggggt gacgccgctt atggcttgct cctcaaagag attaccgact gttatatgtc
     9961 cggaaacaag ttttttaaaa acacaacggg catcttcctg gatgggtcga atcgcataac
    10021 gattgaaaaa aacagtttta atggcaacgg ttggggtatg aggatgcagg ccaattgtat
    10081 ggacaattat atagcccgca ataattttat gggcaatact tttgatgttg ccaccaacgg
    10141 taccttgact cttaacaaat ttgaggaaaa ttactgggat aattatgatg gctatgacct
    10201 gaacaaagat aaaataggcg atgtgcctta cagaccatta agcctgtatt ccgttctcgt
    10261 agaaaagaat ccacccgcta tgttgttgta ccggagcttt atgattacgt tgctcgatcg
    10321 gtctgagcgg ataattccaa gcttgacccc cgagaacttc atcgataacg caccaaaaac
    10381 ggttccttac cagatatgat agaaattaag aatgtcagta aaaagttcgg caaactccag
    10441 gccttggata agcttaatct gaccttgcag cagggagaat gcgttgctct gattggcccg
    10501 aatggctgtg gtaagaccac tctgatcaaa tcaattctgg gtatggtttt gccggacgaa
    10561 ggtgagatta ttttcaacgg aaaatccatt aagcatgaca tcaattaccg gaatgaaatc
    10621 ggttatatgc ctcagattgg ccgctaccct gagaatatga ccatcggcca ggtactggag
    10681 ttgattaaag gcatccgcga tggccatcag gaaaccgata aagagttgta tgagcaattt
    10741 ttgattgcca ccatgctcga gaagaagatg aggacactca gtggaggaac aacccaaaaa
    10801 gtaagtgcag ttctggcatt ccttttccgt cccaaggtgc tcatacttga tgaacctaca
    10861 gcaggccttg atcccatcgc agcactcatc ctcaaggaca agatactcaa agcaaaggaa
    10921 caagggtgcc tgatcctgat cacttcccat ttgctcaacg aattggagga tattgtttcc
    10981 gaaattttgt ttatgcagga aggtaagctg attctgcatg acagcgttga ccggttgaag
    11041 gaacttaccg gtgaagattc catcgcaaga tccatcgttc acctgctaaa agagaatggc
    11101 catgtttaaa gtattgaaat atgttgcatt ggatattttt cgcaacaaga taatcattgc
    11161 ttataccttg atgcttgcat tattctcctg gagtgcattc gctcttgaag acaatgtagc
    11221 aaaaggatta cttactgaat tgaacatcat tctgctgaca gtgccgttgt tttcggtcct
    11281 tttttccacc atttacgtgt ataacagcag cgaatttata gaattgcttg ttagtcatcc
    11341 cgttaaaagg aatgttatct ggacggccct ttttgtgggc ctttctctca gtatggtcgg
    11401 agcgtttatc atcagcaccg gcataccgat aataattttt gctgactttg caaccggttt
    11461 gatgatgata gttgccggtt ccgtattgtc agtgattttc gtcgcaatag cttttctcag
    11521 tagcatcatg gcaagggata aagccaaggg gataggcatc tctatcatat tgtggctcta
    11581 tttcgctttg ctattcgatg gcttggtttt gttcctgttc ctccaatttt cagattatcc
    11641 aattgaaaaa tttgtggtaa tgctgagtgc gctcagcccg attgaccttt gcaggatatt
    11701 ggttttattg agattggacg tatcggccat gatgggtatg acaggtgctg ttttcaagga
    11761 ctttttcgga acaggagggg gcttggctgt gtcttttctg ttactgttga tgtggattgc
    11821 tgtaccgtat ggtatttcat tgcgaaaatt taagcataga gatctttgat atggatatta
    11881 atgtttgata ttttatttct aattagaaaa aatgaaaacg gatattacaa ataaacagga
    11941 tatcttaaag attattctga ttttctatga ccgtgtcaaa accgatgatg tcattggcat
    12001 ttttttcact gaggttgttc agatcaattg ggataagcac attccccata tggtttcctt
    12061 ctgggagagt gtcctctttt atacaggaga ttattcggga agccctatca atacacatcg
    12121 gggtatacat caaaagtata aaacccaacc taagcacttc gcccgatgga ttaaattatt
    12181 caatcaggtc attgatgaaa attattccgg gccgaatgca gagaaaatga aaaaccatac
    12241 catttcgatt gctaaagtta tgcaacaaaa gatctgagtt ttagataaaa tagctatttt
    12301 cagcctgcgc aattcgattt gtgactttca cactgttttc agccaccatc tgaggtaatg
    12361 catgggttga cttgactttg gatgtcatct ttccaactga tgactgtgat tgatttcctt
    12421 gtattcttcc ggctttttcc cacaaaacaa caggctagat ttttattttt ctttgatagg
    12481 tggaaagtga ccccgttttt ataaaaaagt ggggtggggt tataacgatt gacctactgt
    12541 atggaagaaa tagcacagct gaaaaatcat cttggtataa tatatcacaa acttaacata
    12601 cgcctttgtc aacaatcggc aaagtcgata aaaacaaagc tggcatttcg aatattaagt
    12661 tatttaagct atacattaca ctagaatcgt catgattttt tcacggcatg gatttaaggg
    12721 taatcttgtg gaaagaaaaa gaatttgtaa tatataatca atttatttat aacaagttaa
    12781 tatccttttg tgctaagtct atggtttata tttccctgct tttttcttaa taaaagtaaa
    12841 ggacgatttg taatgaacac aatacctgga tgtacttaga cctcatcccc ttattttgat
    12901 tatttttgcc tgggattgaa tgcatgtctg gtttattgat ataccctgat cgggtcagca
    12961 tttataagcc agactacact gttttttttc ttttaggaaa atatttatgg acatcgtttc
    13021 taaagcaatc gaagattatt gtgagtcatt tggagataac ccacccgggc tcttgttaga
    13081 tctggacact tacacccacg agcaccatca tgcacccaat atgttgtcag gtagctttca
    13141 gggtcgggtg ctggcaatga taagccgtgt tgtgaggcct aagaccgtgc tcgaagtagg
    13201 tacctatact ggctattctg cattatgcct tgctgaaggt cttgcggatg gaggtgtagt
    13261 acataccatc gatattgatg aacgtctcag gcagacacat gaactcttca taggccaaag
    13321 ccggtatgct gacaggataa aagtgtattt tggcgatgct aagatactta ttcccgggct
    13381 tggtctaagg ccggatctgg tctttattga tggggcaaaa aaagactatt cagctatttt
    13441 cgatatggta cttcctatga tgaattccgg aggagtgatt ctggccgaca atgtcctttg
    13501 gaaaggaaaa gtactggatg aagaaatgga tgacaggaca gctgcgatcc acgaatttaa
    13561 caggaaggta agtcaatgcg aacttgtata taaagtcctg ctaccggtgc gtgatggcct
    13621 tttctggatt actaagaaat aagatggaat ttctgcgaag aaaggatatt gatacagacc
    13681 gatgggatgc ggtgatcgat agctcggcaa ccggattgcc atatgcatac tcagcagttt
    13741 tggacgaatg ttgcaatggg aaatgggatg ccatagtcgg tgatgcatac aactatgtac
    13801 ttcccttgcc cttcaacagg aaattattcg gcttcaaaca atactatcag ccgtacttgc
    13861 tgcagcaact gggtgtcttt ggcaatatgc cggcgagcgt cgagatggat gccattatgc
    13921 atatgatcaa gagtcacagc atccgaagta tgattgcctt tcaggaagag aatacacaac
    13981 ttcttgcagg tgccggcggg aatttaaaga ccaactttgt tctcgatctt cttccgggct
    14041 atgattacct ttcaagccaa tacaaaaaga atcttaaaca aaggatcgga aggtcgttcg
    14101 atgatgaatt cacaggaagt gaagggcttt ccatggatga tgctatcgat atgtatttcc
    14161 aatggacata tgccaaatac aataaatccg aaaaactatc aaagcgatac atccataatt
    14221 gggtaaggat cttcaaccat cttggttatg cacatgtagt agctctgaga gcgcaggatg
    14281 ccacaattca ttcgggtatc atctacctga agacacactc cagaatcatt ctatcccttc
    14341 agttcagtca tccggattac aaacaatatt caggcccctc cagattgata gattatctga
    14401 tccggcaaca tgcaggcagt aggatgagat tggattttga aggctctcaa atcccatcca
    14461 ttgccgaatt ctatggttct ttcacacctc agaatatgcc ctatacagtg attaagtctt
    14521 agcgactacc aattgtttct tttccttcgg aagctttgaa tgcatcaatt ccacactgaa
    14581 ggaaagttgc gtactgttca ggatcaggtg tatagcctac cggtgtgttt aaaagcttac
    14641 ctgtgtttgt aatcagcgcg tacagcggtt ggctgttgtt gttgaaatta tcggtctgga
    14701 aaaaattcca cttatcgcca accgtcttaa ttcttttcgt acctccattc ttgtatgtca
    14761 aagtgacctg ttggtcttcg ggaagcggtg ttttatcgtc cacataaagc gaaatcacca
    14821 caaattcgtt ggtcagaaga tttttaacct tgttggttgg ccagacatgc tcttccatct
    14881 ttcgacaatt gacacatgca tatcctgtaa aatccagaaa taccggtaag ttgcgggatt
    14941 tggcataggc cagtccttcc tccagatcat ggaagcaatt caggttgttt ggacaatcat
    15001 tagggtacag gatgctgtaa cctaccggag gcgctaagcc gctcagtaat gaaagtgaag
    15061 tgaaggtttt ggtctcttta ttgtagatga aaccggtagc taaataaata gcaaatacca
    15121 gtgatatcaa agccagtaca actcgtgtgg ggcttaactt tttaagtttg ctgtcgtggg
    15181 ggaaacgaat ccatcccatc aggtaggcaa ataaacctaa tgctaccaga atccagatga
    15241 ggaggaacgg ctcgatcttc aataagcccc agtgcttcac cagatccgca ttggaaagga
    15301 acttaaaggc aagcgccacc tcaataaagc cgagtacaac ctttacggta ttcaaccaac
    15361 caccggattt cgggatggta ttcatccaac tggggaaggc tgcaaataaa gcaaaaggca
    15421 atgccagtgc gatgccaaat cctcccatcc cggcagtcag attccacgcc ccaccattag
    15481 aacttaatgt ccctgccagt aaagatccaa ggattggacc ggtacaggaa aacgaaacca
    15541 aggcaagggt cagagccatg aagaaaattc cgattacgcc cccaacacct tcggcagatg
    15601 aaactttatt cgaaagactg ctgggaagcg tgatttcata atatccaaaa aatgaaaatg
    15661 cgaacaccat gaatatcaaa aagaatgcca cattcaacca tgcattggtc gagatgtcat
    15721 tcagaatatt cggattgatg ctgtccagca aatggaacgg aacactcaac aatatataaa
    15781 tcagaaaaat aaaaaatcca tatagaaaag cattggtgat tcctttcttc ttatccttgt
    15841 tactcttcgt gaagaagctt acagtaaggg gaatcatcgg gaaaacacaa ggggtaagca
    15901 acgctatcag acctcctata aagccgagca gaaagatggt aaataatccg gtgctttcct
    15961 ttacattggc ttc
//
LOCUS       DAOQQN010000015        15819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1171_length_15819_cov_5.800051, whole genome shotgun sequence.
ACCESSION   DAOQQN010000015 DAOQQN010000000
VERSION     DAOQQN010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 15819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15819
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1171_length_15819_cov_5.800051"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2723
                     /locus_tag="PLG24_01140"
     CDS             <1..2723
                     /locus_tag="PLG24_01140"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HMZ23514.1"
                     /translation="DASLDVFSRNYFNTIGFPDYHVYYSYSDHHNGLWSLSRHYSMDM
                     QPEIGEQWRNYLDNLLIQWHHTRVYRKMMMSPTMAGGRNFERNAQPEAEMMADAVAGE
                     PTDSNKGAGKEEEPSDQTNHEVVPPSVRSALEETVFFYPDLHTDKNGDVVLRFRMKEA
                     LTRWRLQLFAHTKDLMQATLEEEVTTSKELMVFPNLPRYFRENDKLEVTAKVVNMNGL
                     SGIAHAKLELLDAVTLQPLAGGLIISSAEQTFALSSGQSSVVNWEVQSPALPVEAVTI
                     RITAWTDSHTDGEENTLPVLSNRMMVTETMPIMVRKGQEKTFTFDAMTKANLSGTVTP
                     YRYTLEYSSNPAWYAVQALPYLMEYPYECTEQILSRYYANTLAAYIANSNPKIKAVFE
                     RWKNTDALLSNLQKNQELKSALLEETPWVLEAQSEEQQKKNIGLLFDFNRMANEQQGA
                     LRKLMERQYQSGGLPWFPGCRENEYVTSYVVENLGHLLRLGVIKDDDASIYSFLERAL
                     NYCDQALVRYYEDIKRNNKNDAKKMDENHLSYWPIQYLYARSMMDLGKNDFQQTEAYR
                     YFFNQSKKYWNKKNQYAQGMIALYLHRAGDKTVPGLIVEGMRQTAFRNPNLGMYWKSD
                     YGYFWYQLPIETQALMIEVFSELTQDEDSVDEMKMWLLLNKQTNNWKTTKATSSAIYA
                     LLLQGGAMNLETVYPDITLGGKKLDIPSLKPEPGTGYFKTTYSGKEISKEMQEVKVRN
                     NSRVVNWGGAYYQYFEQLDKIGTFKGTPLTIDKSYNIVRKGDRGETLIPLSSGQVVKV
                     GDLIRVRTIIRVDRPMEYVHLKDMRPAGTEPISNISGYRFKNGLGYYESPKDIANNFF
                     IDYLPKGTYVFEYDIRASLRGNFSTGISTIQCMYAPEFSSHSKGERMEIR"
     gene            complement(2969..3499)
                     /locus_tag="PLG24_01145"
     CDS             complement(2969..3499)
                     /locus_tag="PLG24_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMZ23515.1"
                     /translation="MTKTKWVLDPAHSEISFKVRHMMIASVTGNFENFELSALSDGDD
                     FSNASVSLIADVNSVHTKNEQRDIHLKSADFFDSVNYPHIRFEAENVSLEPGENELNG
                     NLTIRNTTLPVTVHIEYTGTGIDPYGNTKAGFSIEAKVKRKDFGLTWNVPLEGGGVLV
                     SEEVKIIGEFQLVKRP"
     gene            complement(3555..4439)
                     /locus_tag="PLG24_01150"
     CDS             complement(3555..4439)
                     /locus_tag="PLG24_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HMZ23516.1"
                     /translation="MDRKAFLKKSMAGAGLLSVSLAGVNTRISAGNDRAYPDYEALGF
                     NHLPHTNSNIMANIILHKAESRGNADHGWLNSKHTFSFANYYNPDRMHFGALRVLNDD
                     TVAPGMGFGTHPHDNMEIISIPLEGDLEHKDSMGNVAVIRSGDIQVMSAGTGIRHSEY
                     NKNKDAMVKFLQIWVYPNKKNVKPRYDQMTLNRKDRRNKLQQILSPNQDDAGVWIHQD
                     AWFHLGDFDPGQKVTYQIHKNGNGVYLFNLSGTLVVNETMVNKRDGIGIRDTNNIGIE
                     AQSQAEFLIMEVPMEFSL"
     gene            4834..5271
                     /locus_tag="PLG24_01155"
     CDS             4834..5271
                     /locus_tag="PLG24_01155"
                     /inference="COORDINATES: protein motif:HMM:NF019095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HMZ23517.1"
                     /translation="MFKNKFLILFTLFSIAIVSCTPDAEEVLEANNLPMKGTQVVPSN
                     ESNGAGTITGKYSKYTKVFTYTVTWTGLSSPLNGMRFYKGARGVNGPAVKNIQSSGSA
                     AGSITESWTVPDSLRTTLEQGGLYINIQTDNYPNGEIRGQVEF"
     gene            5805..7898
                     /gene="ppk1"
                     /locus_tag="PLG24_01160"
     CDS             5805..7898
                     /gene="ppk1"
                     /locus_tag="PLG24_01160"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HMZ23518.1"
                     /translation="MNKLDSTKFPYIERDISWLEFNYRVLQEARDPSNPLMERLKFIA
                     IFSSNRDEFYRVRVAHHRNIIRLGKKEQKALDFDSNQIMKEILKVVNKQQIELNHTFL
                     NEIIPELKRYGINILRRLDLNEVQIEFIEDYFRDNMLPYVQPVLLVEDRVRPFLNNSA
                     LYLAIHMQLEGVANSGDQYAIVKIPSDHLPRFIEIPPGVQGQRDIIFIDDIVRHSISW
                     LFPGYNIHDTYSIKLTRDAELYIDDEYSGDLATKIRASLNKRSVGPAARLVYDSNMPL
                     KFRNYLKGVFELENIDLFPEGRYHNKKDFFSFPDLGIKDIVAQKMEAVRYKKLEEDST
                     YWDEIKSRDHLLYFPYNSYDPVIKMFELAARDPDVTHIKLIQYRVAEDSKIMEYLIEA
                     AENGTQVSVFIEIKARFDEEANLRWGEKLARAGIKVHYSLPGIKVHSKLALIRRIEDD
                     KPNFYSYLGTGNFNEDTANLYTDFGLFTADVRYTKDVMKVFNYIETGKKPASAFNHLL
                     VGQFNLRSGLEELIRYEINEAQHNRPAKIFIKMNSLQDKAMIDLLYHASQQGVKIKMI
                     IRGICSLVPGVSGVSENIKGISIVDRYLEHARVFIFHNGGNEKVFLSSADWMERNLSY
                     RVETAFPIFNPELKSEIHRLMMIQWEDNMKARTLKHKHVNEYVKNDGDFPNRSQVESY
                     YFIKRREELSMPIED"
     gene            7947..9293
                     /gene="purB"
                     /locus_tag="PLG24_01165"
     CDS             7947..9293
                     /gene="purB"
                     /locus_tag="PLG24_01165"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HMZ23519.1"
                     /translation="MSNLKAISPIDGRYSRQTVELSDYFSEYALIRYRVWVEIRYLLA
                     LQTSPVGNFGLSEEQVKIIRSIGDEFSEDDSLRIKELESITNHDVKSVEYFIKEQLDT
                     EGLSFIREWVHFGLTSQDINNTSFPLMIADAWKNVLGPQLEDLIKIVRDTGMSLVHIP
                     MLARTHGQPASPTTLGKEILVFAERLDNQMKLLKHIPWQGKFGGAVGNMNAHYIAYPG
                     FDWQEWADKFLTSIGLERQHTTTQIEHYDMLAAHFQGITRINNILTDLCRDIWTYISY
                     EYFTQKLKKDEVGSSAMPHKVNPIDFENAEGNLGMANAFFGFLAGKLPVSRLQRDLTD
                     STVLRNVGVPYAHTLIAIKSIVKGWNKLVVNENKIREELQQNSIVLAEAIQTILRREH
                     FDGAYEALKSLTRGRTSISREYLLSFVDSLDVNDEVKAELRKLEVEKYIGGWTPNK"
     gene            9968..11017
                     /locus_tag="PLG24_01170"
     CDS             9968..11017
                     /locus_tag="PLG24_01170"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HMZ23520.1"
                     /translation="MIETKAYAAMSSDSPLAPWSLERRLPGPYDVQIEILYCGVCHSD
                     LHTARNEWHDTKYPVVPGHEIIGRVVAVGIDVVDFQTDDIVGVGCMVDSCSQCESCRE
                     GLEQYCEHGSTGTYNSKERDGSGVTYGGYSGMITVREDFVLRIPGNLPLTGVAPLLCA
                     GITTYSPLRHWKVGKGMKVGILGLGGLGHMGVKLAVAMGAEVTMLSHTATKAEDAERL
                     GAHHFILASDEGQMRSVRSSFDLILDTVSAVHDYNSYLKLLKRDGTMVCLGGPPVGPG
                     IESFNLIGKRRSIAGSLIGGIRETQEMLYFCAEHNIVSDVEVIRMDYINEAYERMLKG
                     DVRYRFVIDMASLKE"
     gene            complement(11699..12049)
                     /gene="ssb"
                     /locus_tag="PLG24_01175"
     CDS             complement(11699..12049)
                     /gene="ssb"
                     /locus_tag="PLG24_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HMZ23521.1"
                     /translation="MNTLRNHVGLTGNLGKAPEIRTFEGGRKMARISLVTNERYTNND
                     GKVVETATWHNLIAWGRPAEIAEKYLTKGREITVSGKLVNRSYIDKNGNKKYVTEIEV
                     NDFHIFGRTSNSVG"
     gene            complement(12403..13278)
                     /locus_tag="PLG24_01180"
     CDS             complement(12403..13278)
                     /locus_tag="PLG24_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ23522.1"
                     /translation="MKLTLQTLSAIILLCLLGTSCQKDTSEPSPYLTSQTYNLKDVAY
                     GTDPQQKMDMYLPANRSSSKTKVFVMIHGGGWSAGDKADYSSTMDALKDYYPNHAIIN
                     INYRLGTQDYPGYPKQIQDIQTALNEISKGKYNLSQQYLLFGGSAGAHLSMLYAYAFD
                     HDHHVKGVINTVGPADLTDPSLTNNLILMGVVGGFLGKAIYDYQGNKALFEETSPARR
                     VTPNAPPTLSFYGDKDPLIPVTQMPKLRTALENNHVWHYDTLYPGGGHGLWASDAQTQ
                     DYITRIIAFIDQFFK"
     gene            complement(13797..15239)
                     /locus_tag="PLG24_01185"
     CDS             complement(13797..15239)
                     /locus_tag="PLG24_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ23523.1"
                     /translation="MNKSFLPQILIIFLIFSEARSQPVFDTVALIAVEEVNTQFCLNN
                     TKLMAGKSMSDRYLVYYNPDTIFLNILQNGTWIRKIAHTGSNIRSATLTTSYDRIWIC
                     WKEGPFIKASYTTDKGNTWSDDFSVSPPGNVSAPSIHASSNGKVHFVWHTESPSDTSI
                     FYRAFNHDMFTTSPQRLSNQEGHGLWPSVISIGDTVLCTWKEAPLPTKVWFRNSFDGG
                     NTWNPLPPEPTTTALTLTKDPNLAYAFDSTTKTHYTYLAYDGHNKIYLQRSTDFGASW
                     SPPDFISNPGKLSQFAHIDCDNKGFIGISYEQRPIGTSLFNDKNKDVGFTFSSNWGSP
                     GSFSTDTLAYTYNNNGSVYAAFNKIDDNSFFLTWLSKDTIENKTFVFERRIHFNHTTD
                     ILSTDSQLPTIDIYPNPFMSRAFIKSDVPLSNASIYVFNASGQLSLQLKNINGKSIEI
                     DRSNLASGVYLVATVQNNRIIATKKIIVAN"
     gene            complement(15508..>15819)
                     /locus_tag="PLG24_01190"
     CDS             complement(15508..>15819)
                     /locus_tag="PLG24_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HMZ23524.1"
                     /translation="VGYYTKQDLTKFGQIMFFGLIGIIIASIVNFFMQNEMMYWIISY
                     VGVAVFVGLIAYDTQKIKAYALMESEEMRRKGAILGALALYLDFINLFIYLLRLFGSR
                     R"
BASE COUNT         4630 a         3044 c         3542 g         4603 t
ORIGIN      
        1 atgatgcatc tcttgatgtg ttttccagaa attattttaa cacgattggc tttcctgatt
       61 accatgtcta ctattcttat tctgatcatc acaacggatt gtggagccta agccgccact
      121 actcaatgga tatgcagcct gagatcggtg agcagtggcg taactatctc gacaatttgc
      181 tgatccaatg gcatcatacg cgtgtgtaca gaaaaatgat gatgagcccg acaatggcag
      241 ggggaaggaa ttttgaaagg aatgctcaac cggaagctga aatgatggcc gatgccgtgg
      301 ctggtgagcc gacagattcc aataaaggtg ccgggaaaga ggaagagcct tccgatcaga
      361 caaatcacga ggtcgttccg ccttcagtac ggtctgcttt ggaagaaacc gtatttttct
      421 acccggatct gcacacagat aagaacggag atgttgtcct tcgattcagg atgaaggaag
      481 cgctaacacg atggagatta cagctatttg ctcataccaa agatttgatg caggccactt
      541 tggaggagga agtcactact tctaaagaat taatggtatt tcccaaccta cctcgatatt
      601 tcagggagaa tgacaagctc gaggtaaccg ccaaggtggt caacatgaat ggccttagtg
      661 gtatcgcaca cgcgaaactc gaacttctgg atgcagttac cttgcagcca ttggccgggg
      721 gattgatcat tagttctgct gaacagactt ttgccttaag ttcaggccag tcaagtgtgg
      781 tcaactggga agtccaatca cctgctttgc ctgttgaagc agtaaccata cgcattacag
      841 cctggactga tagtcatacc gatggggaag agaatacact tccggtactt tccaacagaa
      901 tgatggtgac tgagactatg ccaatcatgg tacgcaaggg acaggaaaaa acttttactt
      961 tcgatgccat gactaaggct aatctatccg gtacagtaac accgtataga tatacactgg
     1021 aatattcgtc caatccggcc tggtatgccg tacaggcgtt gccttacctc atggagtatc
     1081 cctacgaatg tacggaacaa atactcagcc gttactacgc caatacactt gccgcatata
     1141 tagccaatag caatcctaaa atcaaggctg tattcgaaag atggaaaaat acagacgctc
     1201 tgctgagcaa cctgcagaaa aatcaagaac tgaagtctgc cttactggaa gagactccat
     1261 gggtgctgga ggcacaaagc gaggaacaac aaaagaagaa tatcggctta ctcttcgatt
     1321 tcaacagaat ggctaatgaa caacagggcg cgcttcgcaa attgatggaa aggcaatatc
     1381 aaagtggcgg cttgccatgg tttcctggat gccgtgaaaa cgaatatgta acttcctatg
     1441 tagtcgagaa tctgggtcac ttactccgtt tgggtgtcat taaggacgat gatgcttcca
     1501 tatattcctt ccttgagcgg gctttaaact attgtgatca ggcattggta cgatattatg
     1561 aagacatcaa gcggaataat aagaatgatg ccaaaaaaat ggatgagaat catctttcgt
     1621 attggccaat ccaataccta tatgcccgct ccatgatgga tctggggaaa aatgatttcc
     1681 agcaaacgga agcctatcgt tatttcttca atcagtctaa aaaatactgg aacaagaaga
     1741 atcaatatgc acaaggcatg attgcactat acttgcatcg ggccggcgat aaaaccgtac
     1801 caggccttat cgttgaaggc atgaggcaga ctgccttccg caatcccaac ctgggtatgt
     1861 actggaagtc cgactacggc tatttctggt accaattgcc aattgagacc caggctttga
     1921 tgatagaggt gttttcagaa ttgactcagg atgaggacag tgtcgatgag atgaagatgt
     1981 ggctcctgct caacaaacag accaacaact ggaagacaac caaggcaaca tcttccgcca
     2041 tatacgccct gttgcttcag ggaggtgcca tgaatctgga aacagtctat ccggacatta
     2101 ccttaggagg caagaaactg gatataccct cccttaaacc ggagccgggc acaggctatt
     2161 tcaagacaac ttattctggt aaagaaatca gcaaggaaat gcaggaggtc aaagtcagaa
     2221 acaattccag ggtggtcaat tggggaggag cttactacca gtacttcgaa cagctggaca
     2281 aaataggcac tttcaaaggt actcccctca ctatagataa gtcttacaat attgtcagga
     2341 aaggagaccg gggagagacc ttgatacctt tatcctccgg gcaggtagta aaggtaggtg
     2401 atttgatccg tgtgcgaacc attatacgag tggacaggcc catggaatac gtacacctga
     2461 aggatatgcg ccctgcagga acagagccga taagcaatat cagcggctac cgcttcaaga
     2521 acggccttgg atattatgag tcgccgaaag atatcgcaaa taatttcttt atcgattatc
     2581 tgccaaaggg aacttatgtg tttgaatatg atattcgggc atcacttagg ggtaatttct
     2641 ccaccggaat ttctaccata caatgcatgt acgctccgga attttcaagc catagcaagg
     2701 gagaacgaat ggagatccgg taaaggtatt gatgtggtct tgtgttagcc gttgagattc
     2761 aacattgatc tttggtgtaa acaggaaaat ctaactggac atgaaaggtt tgtagggcga
     2821 taaaataaaa gataggatat taagcaaata taaatcgatt tgccaagtaa tatacctgat
     2881 tagtgctcag gccttatgaa aaatcggtca aatgaaaggg gcaaggcata gcggtcttgc
     2941 tcctttcatt tcattcgtct tcagtgaatc atggtcgttt gaccagttgg aactcaccta
     3001 tgatttttac ctcctcactt accaggactc ctccaccttc cagtggaacg ttccatgtga
     3061 gaccgaaatc tttccgcttc actttggcct caatactaaa acccgcctta gtattgccat
     3121 acggatcaat tcccgtaccg gtatattcta tgtggacagt taccggaaga gtagtattac
     3181 ggatggtgag attgccattc aattcatttt cgccgggttc taaggagaca ttttcagctt
     3241 caaatcgaat atgagggtaa ttcacagagt caaagaagtc tgcactcttg agatgaatat
     3301 ccctttgttc gtttttggta tgtacactgt tgacatcggc aatcagggaa acgctggcat
     3361 tgctgaaatc atccccatcc gatagggcac tcagttcaaa attttcgaaa ttacctgtca
     3421 cactggctat catcatgtgc ctaaccttaa atgaaatctc gctgtgcgcc gggtccagaa
     3481 cccatttagt ttttgtcata gttgttatat gtatttagtt attaaaaatg tatcgattgc
     3541 aatggaaatt aatcttatag cgaaaactcc atcggaactt ccattattaa gaattccgcc
     3601 tgactttgtg cttctatacc tatgttgttg gtatcccgga tgcctatgcc atctcgcttg
     3661 ttgaccatag tttcattgac aaccaaggtg cctgatagat tgaaaagata tacaccgtta
     3721 ccatttttat ggatctgata agtaaccttc tggcccgggt caaagtcgcc gaggtgaaac
     3781 catgcatcct gatgaatcca aactcctgca tcgtcttgat tgggcgaaag tatctgttgt
     3841 agcttattgc ggcgatcttt cctattaagt gtcatttgat catatcttgg tttgacattc
     3901 ttcttattag ggtacaccca aatctgaaga aatttcacca tggcatcctt gtttttgttg
     3961 tattcactgt gtcggatacc ggttccggca ctcatgacct gaatgtctcc acttcggatg
     4021 acggcgacat tgcccatgct gtccttatgt tccaggtcac cttccaaggg aatgctgata
     4081 atctccatat tgtcatgtgg atgtgtacca aaacccatac cgggagcgac cgtgtcatca
     4141 ttaagtaccc gcagggcacc aaaatgcatc cggtcaggat tgtaataatt tgcaaaactg
     4201 aaggtatgct tgctgttgag ccagccgtga tccgcattgc cccggctttc cgccttgtga
     4261 agaataatat tagccatgat attggaattt gtatgtggta aatgattaaa acccagagct
     4321 tcataatcgg ggtatgccct atcgttaccg gctgatatcc tggtattgac gcctgccaaa
     4381 gaaacagaca gtaaacctgc cccggccatg ctctttttca aaaaagcctt tctgtccatg
     4441 attcaaaaat tatcaatgca aaggtagggc tataatatgc tgaaaaacaa tatggtatat
     4501 taagaaaaac acttaatcca gattaagaag tgttggcctt tggtatgacc ataatttatc
     4561 attttaaatc agaagtcatc ttcaggcgat aaatgagatt atttgcccac gtatatcaga
     4621 taagtttctt gactattccg gataaattcc ttcacacaac taataatttt tcaaaaatat
     4681 attacaaata attataatat tatatctttg tgtactgaat cacatcatct atacgtttta
     4741 ttattcatca aagaaaaatc atttcattca ccctatccgg catcagcaac cttttgtgaa
     4801 ttttgtttat aaaatcattt aaaccaaaac gttatgttta agaacaaatt cttaatttta
     4861 tttaccttat tttctattgc tattgtttcc tgtactccgg atgcggagga agtgttggaa
     4921 gcgaacaatc ttcccatgaa aggtactcag gtcgttcctt ccaatgaatc caacggtgca
     4981 ggcaccatca ccggtaaata cagtaagtat actaaagtat ttacctatac agtgacatgg
     5041 acgggtctga gtagtccatt gaatggaatg aggttctata agggagctcg tggcgtcaat
     5101 ggcccggctg tcaagaacat ccaatcatcc ggatctgctg ccgggtctat tacagaatcg
     5161 tggacagttc ctgactcgct cagaacaacc ctggagcaag gtgggctgta tatcaatatt
     5221 cagacagata attatccaaa tggagaaatc cgcggacagg tagaattcta acctcttttc
     5281 gggcaatgca aattcgccag gtactcgtac ctgtgaattt gcattgttca caacctgaaa
     5341 tatgtaactg cattttttaa ccgagtcaaa aagatgcacc agattcaatt ttaaccgatt
     5401 cgggatatct gcttttatca ttcaaatcac attattacag gtatatttat tagtaggaag
     5461 agtgcctttg cttttttatt ctactacaaa tacacggaat acacaaggga tgtttgttgg
     5521 atttttagta ccgttacgcg gttcaaaata aatttgtaat acccaagtct gtaatcaaca
     5581 gatgcgtata gagggaagca gaatcatatg ccgttgcaaa ccctgctgta ttaacctcct
     5641 aacgaaatca acgagattgc aagtcttttt aaggtctata atttgtgaaa gtatcttgat
     5701 ggagtagaat ccttgtgaat tattacaatc tgattaaagg tgatatcgga aaaacaaaat
     5761 tttgttttat ttggattaca tttgtgtact tttcggattt taatatgaac aagcttgatt
     5821 ctacgaaatt tccttatata gagcgggata tttcctggct cgaatttaac tatcgtgtat
     5881 tgcaggaggc tcgcgatcct tccaatccat tgatggagcg tttgaaattc attgcgatat
     5941 tttcatccaa tcgggacgaa ttttatcgtg tacgggtggc tcatcatagg aatatcatcc
     6001 gtctgggaaa gaaggaacag aaggctttgg attttgactc caaccagatc atgaaagaaa
     6061 tcctcaaagt tgtcaacaag caacagattg aattgaatca tacattcctg aatgagatca
     6121 ttcctgagtt gaagagatac ggtatcaaca tattgaggcg tcttgatctt aatgaggtac
     6181 agatagaatt cattgaggac tacttcaggg ataacatgtt gccttacgtt cagccggtgc
     6241 ttttggtaga agatcgggtg aggccttttc tgaacaattc ggccttgtat cttgcaatcc
     6301 acatgcagtt ggagggtgtc gccaatagtg gtgaccagta tgccattgtt aagataccat
     6361 cagatcactt acctcgtttt attgagattc cacctggtgt tcaagggcaa cgcgatatta
     6421 tctttattga tgatatcgta cgacatagta tttcctggct ttttcccgga tacaatatcc
     6481 atgatactta cagtataaag ctcacacgtg atgctgaatt gtatatcgat gatgagtatt
     6541 caggtgattt ggcgacaaag atcagagcaa gtcttaataa gaggagtgtc ggtcctgctg
     6601 cgcgattggt gtatgattcc aacatgccct tgaaatttcg caattatttg aaaggtgttt
     6661 ttgagcttga gaatatagat ctgtttccgg aaggcaggta tcacaacaag aaagattttt
     6721 ttagttttcc cgatttggga atcaaggata ttgtagcaca gaaaatggaa gctgtgagat
     6781 acaagaagct ggaggaggac tccacttatt gggatgaaat caaatccagg gatcatcttc
     6841 tttactttcc ctacaattcc tatgatcctg tcataaagat gtttgaattg gccgccaggg
     6901 atcctgatgt gacacatatc aagcttattc aatacagggt ggcggaggat tccaagatta
     6961 tggagtattt gatcgaagct gcggaaaatg gtactcaggt ctcggtattc atcgagatca
     7021 aagctcgttt tgatgaagag gctaatctgc gctggggcga gaagctggca cgggcaggga
     7081 tcaaggtaca ctacagcctt ccaggcatca aggtgcattc caagcttgcg cttatacggc
     7141 ggattgagga tgataaacca aacttttaca gctatctcgg taccggaaac ttcaatgagg
     7201 atacagctaa tctatacacc gattttggac tttttactgc tgatgtacga tacaccaagg
     7261 atgtaatgaa ggtgttcaat tatattgaaa caggcaaaaa gccggcatcg gcctttaacc
     7321 acctgctggt cgggcagttt aatctcagga gtgggctgga agaattgatc cgatatgaga
     7381 tcaatgaggc acagcacaac aggcctgcca agatattcat caagatgaac agcttgcagg
     7441 ataaggctat gattgacctg ctttatcatg catcacagca gggagtcaag ataaagatga
     7501 ttatcagggg catttgttct cttgttccgg gtgtatccgg tgtaagcgaa aatatcaagg
     7561 ggatcagcat tgtggatcga tatctggagc atgcccgtgt ctttatcttc cataacggag
     7621 gcaatgaaaa agtgtttctt tcctctgctg actggatgga acgaaatctt agttacaggg
     7681 tggagacggc tttcccaata tttaatcctg aattgaagag tgagatccat cgtttgatga
     7741 tgatacaatg ggaggacaat atgaaggcaa ggacattgaa gcacaaacat gtcaatgagt
     7801 acgtcaagaa tgatggtgat tttcccaatc gttcccaggt tgaatcctac tacttcatca
     7861 aaagaaggga ggaattgtca atgccaatag aggactgacg attggaatat aaatgtcatg
     7921 aataataaca gagaaaaaaa gaaaccatgt caaacctgaa agctatttcg ccgattgatg
     7981 gtcgatacag taggcagact gtcgaacttt ccgattattt ttcagagtat gccctcatca
     8041 ggtatcgggt ttgggtggaa atccggtatt tgttggcgtt gcaaacttca ccggtgggta
     8101 attttggctt gagcgaagag caggttaaaa ttatcagatc gataggggat gagttttctg
     8161 aagatgatag tcttcgtata aaggagttgg aatcaatcac caatcacgat gtcaagtcgg
     8221 tagaatattt cataaaggag caactggata cagaaggatt gtcctttatt cgtgaatggg
     8281 tacattttgg ccttacttca caggatatca acaatacttc attcccattg atgatagccg
     8341 atgcgtggaa aaatgttttg ggacctcaac tggaagattt gatcaaaatc gtcagggata
     8401 cgggtatgtc gttagtgcac atacccatgt tggccagaac acatgggcaa ccggcatcgc
     8461 cgacgacctt aggcaaagag atactggtct ttgcagagcg attggacaat caaatgaagt
     8521 tgttgaaaca tataccatgg caaggaaaat ttggaggtgc ggtaggtaat atgaatgcgc
     8581 attacatcgc ctatcctgga tttgattggc aagaatgggc agataaattt cttacttcga
     8641 ttgggcttga aaggcagcac accacaaccc agatcgagca ttatgacatg cttgccgccc
     8701 actttcaggg gattacacgg atcaataaca tactaaccga cctctgtcgg gatatttgga
     8761 cttatatcag ttatgagtat tttactcaga agctgaaaaa agatgaagtc ggttcttcgg
     8821 caatgccaca caaggtcaat cccatagact ttgagaatgc tgagggaaat ctgggcatgg
     8881 ccaatgcttt cttcggcttt ctggcgggca aacttccggt gagccgattg cagcgtgacc
     8941 tcaccgacag cactgttctg agaaatgtcg gtgttcctta tgctcacacg ttgattgcta
     9001 ttaagagcat cgtcaagggt tggaataagt tggtagtcaa cgaaaacaaa atccgtgaag
     9061 aacttcagca aaatagtatc gtgttggcgg aggctattca gactatactc cggagggagc
     9121 atttcgatgg cgcttacgaa gccttgaaat cacttaccag gggacgaaca tctatttcaa
     9181 gagaatattt gctgagcttt gttgatagtc ttgatgtaaa tgatgaagta aaagctgaat
     9241 tgagaaagct ggaagtagaa aaatacattg gaggttggac accaaacaaa tagtgacttc
     9301 tcgggcagct actgtgtatg ttagtagttt caggtattta tgcagtaatc atgtgttggt
     9361 attcgtcggt tacgctttgt tccggcttga gaaaaaaatc aacgtcaagg ttgttcctgt
     9421 gggtttatgg aagtggcctg aagttgacat tcgattggag tactgaaatg tcttcttgag
     9481 gagttatctg aaaaaaataa aaggattagg gttgaatcta atccttttaa aaaaagatct
     9541 tatcgatctt gacacacact taattcacaa aagtaatatt aataatcaaa tattgtatga
     9601 aaaattgaaa ataattttat tccgaacgtt gtttttaaat aaggatattt atgtctttta
     9661 atacctaatt aatcgatttg ttgataatac aataataatt tcagagtgta tttatactat
     9721 aattcacgaa aatggagtaa ttagtgaata aaaaatattt ttaaattatt taaataattg
     9781 gataaaaaat atactagata gactgcttag gtcttcggaa aataagtact ttatctcttt
     9841 cagatcagct aacgatttat aaaaagcttt tcgatcttta actttcaggt agattaacag
     9901 ttttataaat aaatcattgg cgtaatagcc tcaaataatt aattatttca ataatcagta
     9961 taatcaaatg atagaaacca aagcttatgc agctatgtct tcagattctc ctctggcacc
    10021 atggagtctg gaaaggcggc ttcccggacc ttatgatgtc cagattgaaa tcctgtattg
    10081 tggagtatgc cacagtgact tacacacagc cagaaatgaa tggcacgata cgaagtaccc
    10141 ggtggttccg ggccatgaaa tcatcggtag agtagtggca gttggtattg acgtggtcga
    10201 ctttcagacg gatgatatag tcggtgtcgg atgtatggtg gactcctgta gtcaatgtga
    10261 aagctgtagg gagggattgg agcaatattg tgaacatggt tcgaccggta cctacaacag
    10321 caaggagaga gatggttcag gcgttacata tggcgggtac agtggcatga ttacggtccg
    10381 ggaggatttt gtcttgcgga ttccgggcaa cctgccttta acaggggtag ctcccttgtt
    10441 atgtgccgga atcaccactt actctccctt gagacactgg aaggtaggaa agggaatgaa
    10501 ggtgggcatt cttggccttg gcggtcttgg ccatatggga gtaaagcttg ctgtagccat
    10561 gggcgctgag gtaaccatgc ttagccatac tgcaactaag gctgaagatg cagagcgctt
    10621 aggggcacat cattttatcc tggcttctga cgaaggtcaa atgaggtctg tacgtagcag
    10681 tttcgattta attcttgaca ctgtttctgc tgtacatgac tacaactcgt acctgaaact
    10741 actgaagaga gatggcacaa tggtttgcct gggcggacca cctgtcgggc ccggaataga
    10801 atccttcaat ctgataggca agcgacgatc cattgccggg tctttgattg gaggtattcg
    10861 tgagacacag gagatgttgt atttttgtgc agaacataat attgtcagtg atgttgaagt
    10921 cattcgcatg gattatatca atgaggctta cgaaagaatg cttaagggtg atgttcgata
    10981 tcgttttgtg attgatatgg caagtctgaa ggaataacct cgacacctca gtttcttgga
    11041 ttgaccaaga ataggtatga agtttatctt ttagagatta tacctagatg aattataaaa
    11101 taatccgata gttgtaggta cacttgtccc gagagctaag cgaccggagc tctgttctgt
    11161 cccggtgtga aacaaaccgg agtttgggga taccgtggtt acagtcgtat agttcaatga
    11221 aattggttta ttacgaatga acattcgata ttacttcaat cgttccaagt ctttcataaa
    11281 atatacaggc ttagggtcag atacaagctt agacgtagat gtcttggata cccgtacaaa
    11341 ataataaaag ccgaaaaaat tgatcttata ccaagatgat ttatataata gtccataaaa
    11401 tctaggaata ctcgtcccgg gagtgaaaca atccggggtt caggtatact tgtcccgaga
    11461 attaagcgac cggagctctg ctcgatcccg gtgtgaaaca aaccggagtt agggaatgcc
    11521 gaggttacag tcgtttagtc aaattatatt ggtttattac taatgcatct tcgttattaa
    11581 ggcacatcct aggtgcattc aaagcctgtc cttgtcattg attcactgaa atcgaagcaa
    11641 gatagattta actaaaaaac cgcaggaagt gctggcttcc tgcggaactt caaaactatc
    11701 acccgactga attggaagta cgaccgaaaa tatgaaaatc attgacttct atttccgtga
    11761 catatttttt attcccgttt ttatcgatat agcttctgtt gaccagcttg ccgctgacag
    11821 ttatttctct acctttggta agatatttct cggcaatttc agccggtctg ccccaggcaa
    11881 tcagattgtg ccaggtggct gtctcaacga cctttccatc gttgttggtg taacgttcat
    11941 tggtgactaa acttattctt gccatttttc ttccaccttc aaatgtcctg atttcaggtg
    12001 ccttccccag attccctgtc aatcctacgt ggtttcttaa agtgttcatg ataataaaaa
    12061 tttaaaggtt acaaaattta agatttacaa tttttattcc gtcattttct ggtgacgttt
    12121 ggtatgacaa agatggaggg agccgaaaga attatccggt atttaaacgt ttgttgtcat
    12181 ttgtaaacga ttataaatgt ttgtaagcaa ttacaaacac ttatactatg ttttgaagac
    12241 ttacaactgc ttatggaaga cgaattgatt acccttaact agctgcagtc gtgaattcaa
    12301 aatactccga attttacgat atatttattg ccagggttga atttcatgga taaattttcc
    12361 gacaatcaat aaattgagag cggccgtttg tcaagctatg gtttacttga aaaactggtc
    12421 gataaaagca ataattcggg tgatgtaatc ctgggtctgt gcatcggatg cccagaggcc
    12481 gtgccctcca cctggataga gggtgtcata gtgccagaca tgattatttt ctaaagcggt
    12541 ccgcagttta ggcatttggg ttacgggaat gagtgggtcc ttgtctccat aaaatgatag
    12601 cgtaggcggt gcatttgggg tcacccgcct ggccggactt gtctcttcaa acaaggcctt
    12661 attgccctga taatcataaa ttgccttgcc tagaaaacca cctactacgc ccatcagtat
    12721 taaattattg gtcagggaag ggtctgtcag gtcagcggga ccaacggtgt tgatcacgcc
    12781 cttcacatgg tgatcgtggt cgaaggcata ggcatagagc atggacaagt gggcaccggc
    12841 tgaccctcca aagaggagat attgttggga taagttgtat ttccccttac tgatttcatt
    12901 gagggcggtc tgaatgtctt gtatttgctt tgggtatccc ggataatcct gagtgccaag
    12961 ccggtaattg atgttgatga tggcgtgatt gggataatag tcctttaagg cgtccatcgt
    13021 cgaggagtaa tcggctttat cgcccgcact ccagccaccg ccatggatca tcacgaacac
    13081 tttggtcttg ctgctactac ggttggcagg taagtacata tccatctttt gttgaggatc
    13141 ggtgccatag gctacatcct tcaggttgta agtttgggaa gtaagataag gagatggttc
    13201 acttgtgtcc ttttggcagg aagtgccgag caggcataag agtataatgg ccgaaagtgt
    13261 ctgcaatgta agtttcatag aataatattt cttgttgacg caggagtgat aaatgacaaa
    13321 cgaagcatta ccctttttcc gtattcaaag ttaatagttt atctttcaat ttctgtaata
    13381 tttatcaagt aaaccagaac ttagatccat ctatcaaatt caacttttag ggcaatagga
    13441 atatgaatca attttttcaa ttgagggtta ggcactccca tctgaaatgg ataattgtgt
    13501 attatgttcc tcgaccgaaa gttgaaaaat ttgagtcatt gaaccgagaa acaaagatat
    13561 gcaatagggt aatttttctc aagagggatt agcaaaatag attgatatac ctaagaatag
    13621 cggatttcat aagcatttaa atcaaatgta agataatggt acatttcctt aagatatact
    13681 gactacaata ttttatcaat aatttgaact cagggttatt gttgcaattt tttcaaaggt
    13741 ttgaatcatt ggattcgtct ttatgatcat gtcaaatttt tgtacctcat tattcttcaa
    13801 tttgcaacaa ttattttttt tgttgcaata atacgattgt tctgtacagt agcgaccagg
    13861 tataccccac tggcgagatt actacgatca atttcaatgg attttccatt gatattcttt
    13921 aactgcaagg aaagttgacc ggatgcattg aaaacgtaaa tggatgcatt gcttaaagga
    13981 acgtccgatt taataaaagc acgtgacatg aaggggttgg gatatatgtc aatggtcggc
    14041 aattgggaat ctgtagataa aatatcggtt gtgtggttga aatggatccg tctttcaaag
    14101 acaaacgttt tattttcgat ggtatctttc gagagccatg tcaggaagaa actattgtca
    14161 tcgatcttat tgaatgcggc atagacagag ccgttattat tgtaggtata tgccaacgta
    14221 tctgtgctga agctgcccgg acttccccag ttggatgaga aggtaaatcc gacatccttg
    14281 tttttatcgt taaagagcga agtgccaatc ggcctttgtt cgtacgaaat gcctataaag
    14341 cctttgttat cgcaatcaat atgtgcaaat tgagataatt tgccggggtt gctgataaag
    14401 tcaggaggag accatgaagc accgaaatcg gtcgaacgtt gtaagtatat cttattatga
    14461 ccgtcatagg caaggtaggt gtagtgagtt ttggttgtcg agtcgaatgc ataggcaaga
    14521 ttgggatcct ttgtcaatgt aagtgctgtt gtagttggtt cgggtggaag cggattccag
    14581 gtgttgccac catcgaatga atttctgaac cagactttag taggcaatgg agcttccttc
    14641 caggtacata aaacagtgtc accaatcgag atgactgaag gccatagacc atgcccctcc
    14701 tgattactca ggcgttgcgg agatgttgta aacatatcat ggttgaaggc tctgtaaaat
    14761 atgcttgtat cagatgggga ttcggtatgc caaacaaaat gtacctttcc attggatgat
    14821 gcatgaatgg agggggcgga tacattgccc ggaggagaca ccgaaaaatc atcactccag
    14881 gtattgcctt tatccgttgt gtagcttgcc tttatgaaag gaccttcctt ccagcatatc
    14941 cagatacggt cataggatgt ggtcagcgtt gctgatctga tattgcttcc ggtatgtgct
    15001 atcttcctga tccaggttcc gttctgtaga atattcagaa aaatggtatc cggattataa
    15061 tataccagat atcggtcgga catggatttt ccggccatca atttggtgtt gttgagacaa
    15121 aattgtgtat ttacttcttc tacggcgatc aaggcgacgg tgtcaaaaac aggctgactt
    15181 cttgcctcag agaatattaa aaaaataatc aatatttggg gtaaaaatga tttattcatg
    15241 atgtgtttag tttaatctaa gcatttgacg ctaccaagcg cagttggttt aaatatctga
    15301 tttgcacgat ccgatcgttt gatgcataac atatcaaaat gccggataga cctggaacag
    15361 acagacccgt cctgtttttt tattacggat cataaaatta ttcggtaaac cggccctgtg
    15421 tgttctgtct tttgtatagc aacctttcta aacaaaaaag gccatccgac ttaacggatg
    15481 gccttgataa tttttaaaat caacttatca acgtctacta ccaaataaac gtaacagata
    15541 aataaataag ttgataaaat cgaggtagag ggcaagtgcg cctaaaatcg cacctttcct
    15601 gcgcatttct tcactttcca tcagagcata tgccttgatc ttctgtgtgt cgtaagctat
    15661 caatccgacg aatactgcca ctcccacata cgatataatc caatacatca tctcattctg
    15721 catgaagaag ttgacgatgg aggcaataat gatgccaata agaccgaaga acattatttg
    15781 gccaaacttg gtcaaatcct gcttggtata atatcccac
//
LOCUS       DAOQQN010000016        15716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1185_length_15716_cov_5.119660, whole genome shotgun sequence.
ACCESSION   DAOQQN010000016 DAOQQN010000000
VERSION     DAOQQN010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 15716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15716
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1185_length_15716_cov_5.119660"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1654)
                     /locus_tag="PLG24_01195"
     CDS             complement(<1..1654)
                     /locus_tag="PLG24_01195"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMZ23525.1"
                     /translation="MSRYVCFFFTLFITVSAFSQRTILRGNVYDKADGNSVIAASVSV
                     IGTNLIDLTDNNGYFNINDVPAGKQKLMVTYIGYDTLLVDVNVVQSGITYKKIFIEQS
                     THVLKEVKITAGSIEKRTEVKAGVITISPKQMKQLPSIGGDADIAQYLPVLPGIVSTG
                     DQGGQIYIRGGAPVQNKIMIDGMTIFNPFHSIGIFSVFETEMIKTIDVNSAGFNVEHG
                     GRVSAVVDIQTKDGNKKRFGGVVGASPFNAKAVLEGPIVKLDENKAFSSSYLLSYKRS
                     LLDKTSKALYSYVNENGLPFAFEDIYGKLNFSFDNGSKIDVFGFNFKDNVNYTATTKF
                     NWKNSGGGINFVLNPVGSSFNINVNANLTNYSIELNEKDQNPRTSSLSNYFVSAAFTN
                     YYPKSEQQFGVEVGGNATNLDYTNYLKNKISQLDNTTEIAFFFKDKFLLNDLVLEPGI
                     RLHYYASLGKFSPEPRIGLKYNANDRLRFKGAAGMYSQNILSTVDDRDVVNLFNGFLS
                     GPDETIYKPNSTTATEHRLQKSIHGVTGFEIDVTNNLDINIEG"
     gene            complement(2026..3273)
                     /locus_tag="PLG24_01200"
     CDS             complement(2026..3273)
                     /locus_tag="PLG24_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ23526.1"
                     /translation="MRLINIFYESFLQAYTELRSNILRTVLSLLGIIIGIFCIIAVQA
                     AVDSLENNIRSSFEKLGNDVIYIDKRPWSDDDRDYWKYMRRPRPSFDDFKAIQARSKL
                     SEISAYTCFIGLKTIKFQKNYVEGAFLMGVSEDYGDLFKIELEQGRFISFNDIVKGTP
                     SIVLGNTVAKNIFGNADPIGRTVNISGNKLQVIGVIAKAGKSLINPLNFDEACIISYK
                     TAGMFINLRGKNFGVSINAKALPGVNIEDLKDELTMIMRSERRLKPIEDDNFALNELS
                     MLDKLFDNVFGILKLAGLIIGGFAILVGMFSVANIMFVSVRERYHIIGIKKALGAARS
                     VILLEFLIESVVLCIIGGIGGLIMVFLVLKISSALTDFEMVLSVTNILRGMLLAVIVG
                     ILAGIIPALKASKLDPVEAIRLQ"
     gene            complement(3370..4140)
                     /locus_tag="PLG24_01205"
     CDS             complement(3370..4140)
                     /locus_tag="PLG24_01205"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HMZ23527.1"
                     /translation="MKTKEEIINNWLPRYTGRSLEEFGKFILLVNFKDYVVRFANTYG
                     VPLYGEDRAMQSASFDDVTIINFGMGSPNAATIMDLLEAITPKAVLFLGKCGGLKKVN
                     KVGDLILPIAAIRGDGTSDDYFPPEVPALPAFALQKAISTAIRENERDYWTGVVYTTN
                     RRVWEWDDNFKDYLFSIRAMGIDMETATIFSVGFHNRIPTGALLLVSDVPMFPEGVKT
                     DKSDMHVNANFVEDHLKIGIRSLLMLKENHQTVRHLKF"
     gene            4255..4941
                     /locus_tag="PLG24_01210"
     CDS             4255..4941
                     /locus_tag="PLG24_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HMZ23528.1"
                     /translation="MHLTESEEDHIRIIFNLYERTGKPVSTNAIAAGMKTTAASVTDM
                     LKRLQVKQLIDYQKYYGVTLNTRGNALAIHLIRRNRLWNVMLRDKLHVKWDQIESITD
                     QLEHINSDMLIEELDRYLGYPQNDVFGDPIPDSNGKFVLRARYPLNEVSFGQQLTVVG
                     ILNHSAEFLKLIDVANIQIGSEIIILEKFDFDQSYLILHKGIEKQLSISIARNLLVRY
                     NNRINHPGKK"
     gene            5009..8779
                     /locus_tag="PLG24_01215"
     CDS             5009..8779
                     /locus_tag="PLG24_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HMZ23529.1"
                     /translation="MRLFFISLVIVLAGTLYSQELWNPVEFNTREETRIKVSKFDFYK
                     LDLTLLKESLTKASNEFQRDNQGVIIEIPMAGKSPQRFRVKQTNTIHESLRSKYPGID
                     NFVIQSVDNPRIHGRIDYTYQGFHAIIKDGSSTTFIDPAFEHNGDYYMVYTRREAINT
                     KSFECYFSKEDDFKPYERMGVSFSDNMLRTYRLALSCTGEYAQFHGGTTPLVLSAMNT
                     TINRVNFVYELEYSVRFEIIPNTDVLIYLNPITDPYDNDDIGKQLDQNQIECDTKIGA
                     ANYDIGHIFSTGGGGLAGYAVVCKNNKAWGGTGSSKPINDPFDIDYVAHEIGHQLSGS
                     HTQNNSCNRDNSACYEPGSASTIMGYAGICSPNVQNNSDAYFHGYSIQEMSQYIVSDF
                     GNQCPTKIQLDGVIPTITTSTGNHTIPKLTPFELKAEADGDSSLIYQWDQLDKGIGET
                     QPPKSTNKISPLFRSYWGNETGVRTFPAINYIVNNTNNIWEVLPGVKRDLNFILTVKE
                     NKAGGANHVQAKNKLAVDNNSGPFLVTYPNQKYITWFVGEQKTITWDVSNTDKSPVNC
                     KNVDILISEDGGYTYPHLLGTFPNIGHADITVPDIEGQKIRVKIAASDNVFFDISNED
                     MVIRRGLPPFGISMDVNPEILCKGDSLEFYVRAISPDTLTDTVSVSINNPNTGLKVSF
                     DSEKFLDNDSIRVTIYNEGAATKANVIEFFFTANGHTIFRQVQIQTYSAPSAPTLYNP
                     VNFSENVTPGLKFVWNAVTGQPVTYDLEVATDIDFFNIVAKRVDVELPQAVLAENLAD
                     NTVYFWRVKANGVCESSPYSGTYVIRTGQCNAFYSTPGKVIELSGSIVSDTITVDSLP
                     FEKLQSLEVIGLKGHTSNIGSIKASLVNADQKSSILWQSSCSGEKEMRLRFSDNAYSE
                     SIPCDTLDQVFGLGDLSVKPVESLSRFNSSTAENTYALVLSMEDSTTTGQMQQWGLNL
                     CGSAPTCKPLRIPTTSRLYTADLACTDDEGWTHYSITAANNSGSNVDLMVMSIKYNGA
                     DPLPADKISVRIPTSARYIRINNAQYVANPANWVVLNRHWLFDQDIQVGNPVDIRFYL
                     TDLEINSMLDAAGLSGQTDSLKVFSVFSNDSINASPAEKHLKVKPNDVKEHTYVLGTY
                     NLNKYLQFSLDYLTDGCVGAGSAILNTNATRSTGVQNIRIFPNPGIDKINIWGINEKT
                     RIEIYDINGRLQKSDRVSGDTVLDLSGLTAGTYLVRLVNNIGQYFEKVNIIK"
     gene            complement(8803..10629)
                     /gene="argS"
                     /locus_tag="PLG24_01220"
     CDS             complement(8803..10629)
                     /gene="argS"
                     /locus_tag="PLG24_01220"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HMZ23530.1"
                     /translation="MDILSVIQNMVSSAFKDNFQSDIAPANIPISLTKPEIKGDYTVT
                     LFPFAKELRTNPNEMGNRMKDALSGKYPEISSIDVVGGFLNFSLSDTYWVDVLHTFNP
                     SPKNEDIKQRFLIEFSSPNTNKPLHLGHVRNMLLGESITNILRELGHDVIKVQVINDR
                     GIAICKSMLAFQLFGEGQTPTQAGIKGDHYVGNLYVLFENKFKEEYKQWQEGPVAGDL
                     LEASKNPDDPGKFFADFKNQYFNTYSKLGLAARDLLNKWEAGDTETLNLWKMMNDWVY
                     EGMNVTYENMGISFDKNYYESNAYLLGKEAVKIGLDRGIFTRDADGSVWIDLTEKGLD
                     RKILLRSDGTSVYITQDIGLAIQRHEEHHFDKMVYVVADEQNYHFQVLFAILKEMGMD
                     WADNLYHLAYGMVELPSGKMKSREGTVVDADDLMAEVIGEAAASGKERGGLASLSVDA
                     QEQIYRKIGIGALKYQMIKVAARKKMIFDPKESVDMQGQTGPYIQNAYVRIQSILRRE
                     RFKGGQILDCNLEDQEVELIKGLTQYSVAINDSAATYDPSQLANYMYILAKNLHRYYH
                     DVPILTAPDETIKQFRLYLIEKVAEVLEAGMRLLGIEMPERM"
     gene            10807..11793
                     /locus_tag="PLG24_01225"
     CDS             10807..11793
                     /locus_tag="PLG24_01225"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HMZ23531.1"
                     /translation="MSRVITLRDALREAMVEEMRLDPTVFLMGEEVAAYNGAYKVSKG
                     LLDEFGPERVIDTPIAELGFAGIGVGAAMNGLKPIVEFMTWNFAVLAFDQIVNNAAKT
                     LSQSAGQFGCPIVFRGPSGAAGQLAQQHSQTFESWMGNVPGVKVISCTDPADAKGLLK
                     SAIRDPNPVCMMESEIFYAEKGEVPEGEYLVPIGKAAVRREGSDVTLVSFNKQVKTCM
                     LAAEELAREGVSAEVIDLRTIRPLDIHTVIESVRKTNRIIVVDESWPMFGVSAEIAYE
                     VQKYAFDYLDAPVTRVNAADTSLGYSHVYVDEYMPNPAKVIKAVKEVMYIKA"
     gene            complement(11975..13600)
                     /locus_tag="PLG24_01230"
     CDS             complement(11975..13600)
                     /locus_tag="PLG24_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ23532.1"
                     /translation="MRSLFLLPIIVFISACSNNSEHNNECVFRYNQPNTVTSLDPAFA
                     RNQSNIWAVDHIYNTLIQLDDSLHVQPCIASDWNIDSSGTIYHLTIRKDITFHDDDCF
                     PNKKGRLLTVDDVIYSLNRIIDTTVASPGSWIFKGRVAENNPFSVVNDSVVEIRLIKP
                     FMPFLQMLSMQYCCIVPKEAVQHYGKTFRQHPVGTGPFQMVRWIEGQTMILKKNPNYF
                     EFINEKRLPYVDIVRISFIGDKKTAFLQLLEGNLDFLSGFDPSMASEIFTTNGTLRSR
                     YSDKIQCYKAPYLNTEYLGINLDWPTDNFLHIKKFRQALNFGFDRQGLLETIRQGIGT
                     PAISSFPPSGLPSYNPGEVQGYRYNPSRARQLLKESGYFNLPVEKRPILSLYTAKEYS
                     DYCLYIAKQWEQLGVRCKVELAESATVREMMRNGKILFFRGSWLADYPDAENYLTVFY
                     SKNPAPPNYTRFKNAEYDRLYEASLICKDEKERFQLYQRMEKILVEESPVIFLYYDAI
                     TNFTSPMIKDYMPNPLNLLKVKYIKKDCRNPIK"
     gene            complement(13703..14173)
                     /locus_tag="PLG24_01235"
     CDS             complement(13703..14173)
                     /locus_tag="PLG24_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HMZ23533.1"
                     /translation="MQFIFWTIGKTNSAEFAKWENDFTKRIKSFTPFDSDVLDAGRGL
                     KNPDQIRAEEGKKVLSRLKTEDYLILLDEKGKTFTSREFANYLEKKMSTSSGKRIVFL
                     VGGAYGFHESIYSRANDKISLSAMTFSHQLIRQIFLEQLYRAFTIINNHPYHNE"
     gene            complement(14179..>15716)
                     /locus_tag="PLG24_01240"
     CDS             complement(14179..>15716)
                     /locus_tag="PLG24_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23534.1"
                     /translation="ISEEKGSINATYTDHGLPQIHARINKTPLLILNFQCDCLSFNEQ
                     KGCKHITGILYYLRNKSVRLTPVGSRRRVGVDELLSVLQKQEMESFIRFYASNNPSFS
                     KIFKQFFSYKFNSYGKGFNDYITEISHSYINVAGKLDSKGVRALYQIYEMHILRTDTM
                     LAAKDWTGAVEVIEPILLQYFKVDFEHSVRSATQLLSKAFQNLSIIINQPIAPGLKRQ
                     IQKLMLTLLEKEHYNYFLSPNAIDIGLSDENQDIQGFRTTIINRIKKKNGEFDGNWLW
                     QLFQIDIRTDSFNLIPFYAEYLDEPSGLESFLKILIEKDHTSPLIDRIIEIVIKKGNL
                     LFGRNSNLLAGYLIRNKKIDLYRLFFERIISLGIDIEKITGYVELNFAIQQVDFAQAL
                     SESNSFRQIKASHPDLYMEIIFKGKNYNEALDLLSNGVPVAEMVPYVGVLTCHDPERM
                     SLLLKDKLKHFLDSHLGIASKGLVEEILSELEQFKQSNVADEIREFVRKNYPERKTLI
                     KTL"
BASE COUNT         4659 a         3091 c         3165 g         4801 t
ORIGIN      
        1 agccttcgat attgatatcc aggttattgg tgacatcgat ttcaaaaccg gttacaccgt
       61 gaatggactt ttgcagccgg tgttcggtgg ccgttgtgga attgggcttg taaatggttt
      121 catcaggacc ggaaaggaag ccgttgaaaa ggttgacaac gtccctatca tccactgtac
      181 tgagaatatt ttgagaatac ataccggcag cgcccttgaa cctaagtcta tcgttggcat
      241 tgtatttcaa tccaatcctt ggttcagggc taaatttgcc taaagaagcg taataatgaa
      301 gcctgatgcc gggttccaga actaaatcat ttaaaagaaa cttgtcctta aagaaaaagg
      361 caatctcggt tgtattgtcc agttggctaa tcttgttttt caaataattg gtgtaatcca
      421 gattggtagc attccccccg acttcaacac caaattgttg ttcagatttg ggataatagt
      481 tggtaaatgc tgcagatacg aaataatttg aaagtgagga agttctggga ttctggtctt
      541 tctcattcaa ttcaatgctg taattggtta gattagcatt gacattgata ttgaaacttg
      601 agcctaccgg attgagtaca aaattgattc cgcctcctga atttttccaa ttaaatttgg
      661 tagtcgccgt gtaattgaca ttgtctttga aattgaaccc gaagacatcg attttacttc
      721 cattgtcaaa agaaaaattt aacttcccat agatgtcttc aaaggcaaat gggagtccat
      781 tttcattgac atagctatag agcgccttgg aggttttatc caataatgat cttttgtaag
      841 aaagcagata tgaggaggaa aatgctttat tttcgtccag ttttacgatt ggaccttcaa
      901 gaactgcttt tgcattgaat ggacttgctc caaccacacc gccaaacctc tttttgttac
      961 catctttagt ctgtatatcg acgacggcgg atacccgtcc gccatgttca acgttgaagc
     1021 ctgctgaatt gacgtcaatc gttttgatca tttcggtttc gaaaactgaa aagataccga
     1081 tactatgaaa agggttaaaa atggtcatgc catcaatcat gatcttattc tggaccggag
     1141 caccacctcg gatgtagatc tgaccaccct gatcacccgt acttacgata ccgggtagaa
     1201 ccggcaaata ctgtgcaata tcagcatctc ctccgataga aggaagctgc ttcatttgtt
     1261 ttggagaaat ggtaattact ccggctttca cttcagtcct tttttcaatg gaacctgcag
     1321 taatcttaac ttccttcaat acatgcgtag actgttcgat gaatattttc ttataagtga
     1381 ttccggattg tacaacattg acatcaacta gcaaggtatc ataaccaata taagtcacca
     1441 tcaacttctg tttgccggcc ggtacgtcat taatattaaa ataaccattg ttgtcagtta
     1501 agtctatcaa attggttcca atgacagaga ctgatgctgc aatgacggag ttcccatctg
     1561 ctttatcata tacattacct cgaagaatag ttcgttggga gaatgccgaa acagtaataa
     1621 aaagggtaaa aaagaaacaa acgtaccttg acactttata aaatatttta gaagacgtaa
     1681 aagtagtttt ttttatgatt ttcaaaatgc ttatttgtaa taattaatct tttaacatat
     1741 catctaattt gatgtagcac tcgatagttg attgattagg ctattttaat tttttcttta
     1801 taaatcttta taataatgag gtaaccaata tcatatatat tgttgaaaat ttgcataaaa
     1861 ttatgaagtg cttgacccac aagttgctat tatccacttc ctcatataca atttcgggac
     1921 aataattgaa aattccatta ttcccttaga aactgacctt tgcctatgga taatttgtag
     1981 attctaaaac tatgaaaatt taacattcct gtctaataac catcgctatt gtaatctgat
     2041 tgcttccacc ggatccagtt tagaagcctt tagtgctggt atgattcctg ctaagatacc
     2101 cactattact gcaagcagca tgccacgtaa tatattggta acagaaagta ccatttcaaa
     2161 atctgtcaga gcggatgaaa tctttaaaac cagaaaaacc ataatcaggc cgccaatccc
     2221 cccgattata catagaacaa cggattcaat gagaaattcc aataatatga cacttctggc
     2281 agctccaagt gcttttttaa tcccaataat gtggtaacgc tcccggactg aaacaaacat
     2341 aatattcgcc acactgaaca tgccaactaa tatagcaaac ccaccgatga taagtcctgc
     2401 cagtttcaga atgccaaaga cattgtcaaa cagtttatcc aacatagata gctcattgag
     2461 agcgaaattg tcatcttcaa taggtttcaa ccgtctttcc gatcgcatga tcatagtcag
     2521 ttcatccttc aggtcttcaa tattcactcc cggcagtgct ttggcattga tggatacgcc
     2581 aaaattcttc cctctaagat tgataaacat acctgctgtt ttataggaga tgatgcatgc
     2641 ttcatcaaaa tttaaagggt ttattaaact ctttcctgct tttgcaatta ccccgataac
     2701 ctgtagttta tttccactga tgtttacggt acgtcctatc ggatccgcgt taccgaaaat
     2761 gttttttgca actgtgttac cgagcacgat ggagggcgta cctttgacga tatcattgaa
     2821 ggaaataaat ctgccttgtt ccagttctat cttgaaaagg tcgccatagt cttcacttac
     2881 acccattaaa aatgctcctt ctacatagtt tttctggaat ttgatggttt tcagtccaat
     2941 gaagcaagta taggcactaa tttctgacaa tttacttctg gcctgaatcg ccttaaagtc
     3001 gtcaaaggaa ggcctgggcc ttctcatgta cttccaatag tcccgatcat catcagacca
     3061 tggtctttta tcgatataaa ttacatcatt gccaagtttt tcaaaactac tcctgatgtt
     3121 attttcgagt gaatcaacag ctgcctggac tgctataatg caaaaaatgc caataattat
     3181 gcccaacagt gaaagtactg tgcgaagtat attgcttctc agttcggtat atgcctgaag
     3241 gaagctttca tagaatatgt tgatcaacct caaaatatta aatttttact cggtttgtca
     3301 aaaatactct attctatatg tggaaaagca atttttcgac aatcatcctt gaattctata
     3361 tcaatggcat taaaatttga ggtgcctgac ggtctggtga ttttctttca gcatcaaaag
     3421 cgaccttatc ccaatcttca ggtggtcttc tacaaaatta gcattgacgt gcatatcact
     3481 tttgtccgtc tttacgcctt cagggaacat ggggacatca ctcaccagta gcagtgcccc
     3541 ggtcggtatt ctgttgtgaa atcctacgga aaaaatagtc gcggtctcca tgtcgattcc
     3601 catcgctctg attgaaaaga gataatcctt gaagttgtca tcccattccc aaacacgtct
     3661 gttggttgta tatacgacac ctgtccaata atcacgttca ttttcacgaa tagctgtgga
     3721 aatcgctttt tgcaatgcga atgccggaag agcaggcact tcaggtggaa agtaatcatc
     3781 tgaagtaccg tcacctctga tggcagctat aggaaggata aggtcgccaa ctttattcac
     3841 cttcttcagg ccgccacatt tacccaaaaa caaaacagcc tttggggtga tggcctccag
     3901 caggtccatg atcgttgctg catttggact tcccatgccg aaattgatga tggttacatc
     3961 atcaaaactt gcactttgca tagcacggtc ttcaccatac aaaggtaccc cataggtatt
     4021 ggcaaatcgt acaacgtaat ccttgaaatt gaccaataaa ataaatttac caaactcttc
     4081 taaagatcgt ccggtgtatc tgggtaacca gttattgata atttcctctt ttgtcttcat
     4141 cagtatttgc tttttaaaag tggcaaatta taaaaatatt caaagacttg gaaataaacc
     4201 tggaatgttg gctctttttt ttctaacttg caaccttcca aattcagtgt cagtatgcat
     4261 ttgacggagt cagaagaaga tcatatcagg ataattttca atctttatga aaggaccgga
     4321 aagcccgtgt cgaccaatgc aattgctgca ggtatgaaga caacagcagc atctgtgact
     4381 gatatgctca aacgattgca ggttaaacaa ttgattgatt atcaaaaata ttatggagtc
     4441 actctcaata ccagggggaa tgctttggca atacatctta tacgcagaaa ccgattgtgg
     4501 aatgttatgc tcagggataa acttcatgtc aaatgggacc aaattgaatc tataaccgat
     4561 caattagagc acatcaacag tgacatgtta atcgaagaac tggacaggta tttggggtat
     4621 cctcaaaatg atgtatttgg cgatccaatt ccggattcta acggcaagtt tgtcttgcgg
     4681 gcaagatatc cattgaatga agttagtttt ggacaacaac tcaccgtagt aggtatttta
     4741 aatcatagtg ctgaattcct gaaattgatc gatgttgcaa atattcaaat cggcagtgaa
     4801 ataataattt tggaaaaatt tgactttgac cagtcttatc tcattcttca taaaggaatc
     4861 gaaaaacaat tgtcgatttc aattgcccgc aatcttttag tcagatacaa taacagaata
     4921 aatcatcccg ggaagaaata aaatgttcat gagcataaat tcttcatata tttaccttta
     4981 attaacagaa acctataata caacaagaat gcgtttattt ttcatttctt tggtaattgt
     5041 acttgccgga accctttaca gtcaggaatt gtggaatcca gtcgaattta atacgcgaga
     5101 agagacaaga atcaaagtca gtaaatttga tttttacaag cttgacctca cgttgttgaa
     5161 ggaaagtttg acgaaagcct ccaacgaatt tcaacgggac aatcaggggg tcattattga
     5221 aatacctatg gcagggaaga gtccacaaag gttcagagtc aagcagacaa atacaattca
     5281 tgaaagtctg cggagtaaat atccgggtat tgataatttt gttattcagt cggtagacaa
     5341 tccccggatt catgggagga ttgattatac ttaccaggga ttccatgcca tcataaagga
     5401 tggaagtagt accaccttca ttgatcctgc attcgaacat aatggtgatt actatatggt
     5461 atatactaga cgggaggcca tcaatacaaa gtctttcgaa tgttattttt ccaaagaaga
     5521 tgattttaaa ccatatgaaa ggatgggtgt cagcttctct gacaatatgc ttagaaccta
     5581 tcggttggct ctttcctgta ccggtgaata tgcgcagttt catggaggta ctacgccatt
     5641 ggtattatct gcaatgaata ccacgatcaa cagagttaat tttgtatatg aactggaata
     5701 tagcgtaagg tttgagatta ttcctaatac cgatgtgctt atatatttaa atcctattac
     5761 tgacccatat gataatgatg atatcgggaa gcaattggat cagaatcaaa tagaatgtga
     5821 tacgaagata ggggccgcca actatgacat tggtcatatt ttcagcactg gtggaggtgg
     5881 attggcaggt tatgcggtag tatgcaagaa taataaggcc tggggaggca caggttcttc
     5941 caaacccatt aatgatccat tcgatattga ctatgttgcg cacgaaattg gtcatcaact
     6001 aagtgggtca catacccaga acaatagttg caacagggat aattctgcct gttatgagcc
     6061 gggaagcgca tctacaatca tgggttatgc cgggatctgc tcaccaaatg ttcagaacaa
     6121 cagcgatgct tatttccatg gatacagcat ccaggagatg tcgcaatata tagtaagtga
     6181 ttttggtaac caatgcccta cgaaaattca acttgatgga gtcataccaa ccattacaac
     6241 atcaacaggt aaccatacca tacccaaatt aactccgttt gaattaaagg ctgaggctga
     6301 tggtgattca tctttgatct atcaatggga ccaattggat aaaggaattg gagaaaccca
     6361 gccacctaaa tctactaata agatcagtcc tctgttcaga tcttactggg gtaatgagac
     6421 gggtgtaagg actttcccgg caatcaatta cattgtcaat aatacaaata atatatggga
     6481 ggtgcttccc ggtgtcaaac gggatttgaa ttttattctt actgtcaagg agaataaggc
     6541 aggtggggct aatcacgtgc aagccaagaa caaacttgca gttgataata acagtggtcc
     6601 ttttctagta acctatccca atcaaaaata tattacttgg tttgtcggag agcaaaaaac
     6661 cataacctgg gatgtttcaa atacagataa aagtcctgtc aactgtaaaa atgtggacat
     6721 attgatctca gaagatggtg gctatacata tcctcacctg cttggcactt ttccaaacat
     6781 cggacacgct gatataacgg ttcctgatat tgaaggtcag aaaatcaggg ttaagattgc
     6841 cgcatctgat aatgtgtttt ttgatatatc caatgaagat atggtgataa ggagaggatt
     6901 accgcctttc ggaataagta tggatgtcaa tccggagatt ttatgtaagg gtgacagtct
     6961 ggagttttac gtgagggcta tttcacctga cacattaacg gatactgtga gtgttagcat
     7021 caataatccc aatacaggat tgaaagtgag ttttgattcc gaaaaatttt tggacaatga
     7081 ttccatccgg gttacaattt ataatgaagg agcggccaca aaggccaatg tgatcgaatt
     7141 tttctttaca gcgaatggtc ataccatttt ccggcaagtt caaattcaga cttattcagc
     7201 gccgtcagct cctaccctat acaatcctgt caatttcagt gagaatgtca cacccggcct
     7261 taaattcgtc tggaatgcag ttaccgggca acctgtaact tatgacctgg aggttgccac
     7321 agatatagat ttcttcaata ttgtcgcaaa aagagttgat gttgaattgc ctcaagccgt
     7381 attagctgaa aatcttgcag acaatactgt ttatttctgg agagtaaaag caaatggtgt
     7441 atgtgagagc tcaccttatt ccggaacata tgtgattcgt acaggccagt gcaatgcatt
     7501 ctattcgaca ccagggaagg tgatcgaact tagtgggtca attgtgtcag atacgattac
     7561 ggttgacagt cttccgtttg agaagcttca aagccttgaa gtcattggat tgaaagggca
     7621 tacttcgaat ataggttcaa tcaaagccag tctcgtgaat gctgatcaaa aatcttccat
     7681 tttatggcaa agcagctgtt caggcgaaaa agagatgagg ttgaggtttt cggataatgc
     7741 ttattcagag tccattccat gtgatacgct tgatcaagtg tttggattag gtgatctttc
     7801 agtcaaacct gtcgagtcct tatcaagatt taattcatca acagcggaaa acacctatgc
     7861 gctcgtcctt agtatggaag attcaaccac tacaggacaa atgcaacaat ggggactaaa
     7921 tctgtgtgga agtgcaccaa cttgtaaacc attgaggatc ccaactacat ctcgtcttta
     7981 tacagctgat cttgcctgta cagatgatga gggttggaca cactatagta ttaccgcagc
     8041 caacaattca ggcagtaatg tagaccttat ggttatgtcc atcaaatata acggagcaga
     8101 tccattgccg gctgataaaa ttagtgtgag aatacccact tctgccagat atattagaat
     8161 aaacaatgca caatatgttg caaaccctgc caattgggtt gttctaaaca gacactggct
     8221 gtttgatcag gacattcagg ttggtaatcc ggtggatatc agattctacc ttaccgatct
     8281 tgagatcaat tctatgcttg atgcagccgg tttgtccgga cagaccgata gtcttaaggt
     8341 gttttccgtt tttagcaacg acagtattaa tgcgagtccg gcggaaaaac atctcaaggt
     8401 caagccaaat gatgtaaagg aacatactta tgttttgggt acatataatc taaacaaata
     8461 tctgcaattc tctcttgatt atcttactga tggttgtgtc ggagctggta gcgctatatt
     8521 gaatacaaac gcaaccagga gcaccggagt acaaaatatt cgaattttcc ctaatcctgg
     8581 catcgataaa atcaacattt ggggtatcaa tgagaaaacg cgcattgaaa tctatgatat
     8641 aaatggcaga ttgcagaaat cagacagagt atccggagat accgtactcg atttatcagg
     8701 attgacggca gggacttatc tggtaagatt ggttaacaat atcggacagt atttcgaaaa
     8761 agtgaatata attaagtaga gcctatcgtc tgaagccgaa tgttacattc gttcaggcat
     8821 ctctataccc aataagcgca ttcctgcttc cagtacttct gcgacttttt ctatcagata
     8881 gagtctgaat tgcttgattg tctcatccgg tgcagtaaga atcgggacat cgtgatagta
     8941 tcggtgcagg tttttggcca aaatatacat ataattggcg agctgggaag gatcataagt
     9001 cgcagcggag tcgttgatgg caactgagta ctgtgtgagc ccctttatta actctacctc
     9061 ctgatcttct aagttacagt ccagtatttg tccgcctttg aagcgctccc ttcttaggat
     9121 gctttgaatt ctaacgtatg cattttggat atagggacct gtctggcctt gcatatcgac
     9181 agattccttt gggtcgaaga tcattttttt gcgtgcagcg accttgatca tttggtactt
     9241 caaagcgcct atgccaattt tacgatatat ttgttcctga gcatcaacgg atagagaagc
     9301 cagcccgcct ctctccttac ctgaggcagc agcctcaccg attacttctg ccattaaatc
     9361 gtctgcatct acaacagtgc cttctctgga tttcatttta ccgcttggca actcaaccat
     9421 gccatacgca agatggtata aattgtctgc ccaatccatg cccatttctt taagaatagc
     9481 aaataatacc tggaaatgat aattttgctc atcagcaacg acgtatacca ttttgtcaaa
     9541 atgatgttct tcatgacgtt ggatagccaa accgatatcc tgagttatat atactgaagt
     9601 tccatctgac cgaagcagga ttttcctgtc aagacctttt tcggtcaaat caatccatac
     9661 cgaaccgtca gcatcacgcg taaaaattcc cctatccagg cctattttga cagcctcttt
     9721 acccaatagg taagcattgc tttcataata gtttttatca aaggagatgc ccatattttc
     9781 atatgtcaca ttcatacctt catacaccca atcgttcatc attttccaca gattgagcgt
     9841 ttccgtgtca ccggcttccc atttgttaag caaatcgcgg gcagcaaggc ctagtttgct
     9901 gtatgtattg aaatattggt ttttaaaatc cgcaaagaac ttaccgggat catcaggatt
     9961 ctttgaagcc tcgagtaggt ccccggctac agggccttcc tgccattgtt tgtattcttc
    10021 cttgaattta ttctcaaaaa gaacgtacaa gttaccaaca taatgatcgc ctttaatgcc
    10081 ggcctgggtg ggtgtctgac cctccccaaa taattgaaat gccagcatgc tcttacagat
    10141 cgctatgcct ctatcattga ttacctgtac ttttatgaca tcatgtccaa gctcgcgaag
    10201 gatgttggtt atcgattctc ccagaagcat attccgtaca tgccccagat gtaagggttt
    10261 gttagtattg ggcgaggaaa attcgatcag aaatctttgt ttgatatcct catttttggg
    10321 gcttggatta aatgtatgca ggacatcaac ccaataagta tcagaaaggc tgaaattcag
    10381 aaagccaccg acgacatcaa tggatgatat ttcagggtat ttgccggaca aagcatcttt
    10441 catccgattt cccatttcat ttggatttgt tcttaactct ttggcaaatg gaaacagtgt
    10501 aaccgtatag tcacctttta tctcgggctt tgtcaggctt attggaatgt tagccggtgc
    10561 aatatcggat tggaaattat ccttgaatgc tgagctgacc atattttgta tgacagaaag
    10621 aatatccatt cgtacttatt aattttaatt agggtgcaaa cttacatatt ttttttacag
    10681 caaaggataa ggctggccta acttaggcag gtccaattac aggaagaaaa aggggaatat
    10741 tggaaaaatc tgccttggct tgtattgaaa atatgttacc tttgcggcaa aattattaaa
    10801 aatagaatgt caagagtaat aacattacgg gatgcattga gagaagcaat ggtagaagaa
    10861 atgcgtcttg atccgacggt atttctcatg ggggaagaag ttgctgctta taatggtgca
    10921 tataaggtga gtaagggttt gttggatgaa tttggtcctg aaagggttat tgatacgcct
    10981 attgctgaac ttgggtttgc cggaatcggc gtcggagcag ccatgaatgg ccttaaaccc
    11041 attgttgaat ttatgacatg gaatttcgct gtgcttgctt tcgatcagat cgtcaataat
    11101 gcagcgaaga cacttagtca atccgcaggc cagtttggtt gtccgattgt gttcagggga
    11161 ccatccggtg ccgccggcca attggcgcaa cagcacagtc agacttttga gtcctggatg
    11221 ggtaatgttc ccggcgtaaa agtgatttct tgtacagatc cggctgatgc caagggactt
    11281 ctaaaatctg caatcagaga tcccaacccg gtatgtatga tggagtctga gattttctat
    11341 gctgaaaagg gtgaagtccc ggaaggcgaa tatcttgttc caatcggtaa ggcagcagtc
    11401 aggcgagaag gaagtgatgt tactcttgtt tctttcaaca aacaagttaa gacctgtatg
    11461 ttggctgcag aagaacttgc cagggaaggt gtttctgcag aggttattga cttgaggacc
    11521 atcagacctc ttgatatcca tactgtcatc gagtcggtca ggaaaaccaa tcgcatcatt
    11581 gttgtcgatg aaagttggcc gatgtttggt gtctctgctg aaattgcata cgaagtacag
    11641 aagtacgcct ttgattatct ggatgcacct gtgaccaggg tcaatgccgc cgatacttca
    11701 cttggttatt cccatgtata cgtggatgaa tatatgccta atcctgctaa agtgataaag
    11761 gcagtaaaag aggtgatgta cattaaggct taaaagtttt tgagttaata aatataaaca
    11821 ggttcttttg agcctgtttt ttttataccc caaattatca tagcttttgg ggtaaatttt
    11881 ctgaatcagg ttagcttacc aaaaaacttc ctattgtacc ggtatgttaa ccaaagtaat
    11941 aggtaattat agaagaaatc tttctacatg gatatcactt gattgggttc cggcaatctt
    12001 ttttaatata tttaactttt aacaaattaa ggggattagg catataatcc ttaatcatag
    12061 gtgatgtgaa attagtgatg gcgtcataat ataaaaagat aaccggactt tcttctacta
    12121 aaatcttttc cattcgttga tataattgga atctttcctt ttcatcctta caaattaggg
    12181 acgcctcata cagcctgtca tattctgcat ttttgaaacg ggtataattg ggtggtgcag
    12241 gattcttgct ataaaatacg gtaaggtaat tttcggcatc cggatagtca gcaagccagc
    12301 ttcctctgaa aaacaagatc ttgccattgc gcatcatctc cctgactgtt gctgattctg
    12361 ccagttccac cttgcaccgg acccccaatt gctcccattg cttggcaata tagagacaat
    12421 aatctgaata ttccttggca gtgtatagag aaagtatagg tcgtttttca acagggagat
    12481 tgaagtatcc gctttctttc aacaattgtc tcgccctgga aggattatat ctgtaacctt
    12541 gcacttctcc gggattatac gaaggaaggc cggaaggggg aaacgaggat atcgcaggcg
    12601 tacctatacc ttgccgtata gtttcaagta atccctgtct gtcaaaacca aaattgaggg
    12661 cttgtctgaa ttttttaata tgcaggaaat tgtctgtcgg ccagtcgaga ttaatcccca
    12721 gatactctgt atttaaatag ggtgctttat aacattggat cttatcgcta tatctggaac
    12781 gtaaagtacc attggtagta aatatttcac ttgccattga tggatcaaaa ccgctcaaaa
    12841 agtcaagatt gccttccagt aattgtaaaa atgccgtctt cttatcgccg ataaaagaaa
    12901 ttctcacaat atcaacataa ggtagtcgct tttcatttat gaattcaaaa tagtttggat
    12961 tttttttcag gatcatcgtt tgaccttcaa tccatctgac catttgaaaa gggccggtgc
    13021 caacaggatg ttgcctgaat gtttttccat agtgttggac ggcttctttt ggtacaatac
    13081 agcaatattg catacttaac atttgcaaaa aaggcataaa tggtttgatg agtctgattt
    13141 ctactacaga atcattgacc acactaaagg ggttattttc agcaaccctg cctttaaaga
    13201 tccagcttcc cgggcttgca actgttgtgt cgatgatccg attaaggcta tatataacat
    13261 catccacagt tagcaatctg cctttcttat tgggaaaaca atcatcgtca tgaaaagtaa
    13321 tatccttacg tatggtaagg tggtagatag ttccgctgga atcaatattc cagtcagaag
    13381 ctatacaggg ttgaacatgc aggctatcat ccagctgtat caacgtattg taaatatggt
    13441 ctactgccca gatattggat tgatttcggg caaaagccgg gtccagggaa gtcaccgtat
    13501 tgggctgatt atatcggaat acgcattcat tattatgctc cgaattgttg ctacatgcac
    13561 taataaacac aataatcggt aacaggaaca ggcttctcat cttcgattta atctacaaag
    13621 aaagtaaaaa ttcaaggata aaatgcattg atcagctact tgatcaggta ctaaggtatg
    13681 tattaataac attagaagtt atttattcat tatggtatgg gtggttgttg atgatggtaa
    13741 aggctctgta taactgttcc agaaaaattt gacgaatcaa ttgatgggaa aatgtcatgg
    13801 ctgaaaggga aatcttatca ttggctcggc tgtagattga ttcatgaaaa ccataggcac
    13861 ctcccaccag gaagacgatc cgctttccgg atgatgtcga catttttttt tccagatagt
    13921 tggcaaactc ccggcttgtg aatgtttttc ctttttcatc caacaaaatc aaatagtctt
    13981 ccgtttttaa tcgcgacaat accttttttc cttcttcagc cctgatttga tcgggatttt
    14041 ttaagccacg accggcatca aggacgtccg agtcaaaagg agtaaatgac ttaatccttt
    14101 tagtaaaatc attttcccac tttgcaaatt cagcagaatt ggtcttgcct atggtccaga
    14161 aaatgaattg catggttgtc aaagggtttt aatgagtgtt ttcctttccg gataattctt
    14221 ccgcacaaat tctcttattt catccgccac attagattgc ttgaattgtt ccagttctga
    14281 gagtatttcc tcgaccaggc cttttgaagc gatgcccaaa tggctatcca gaaagtgctt
    14341 aagcttatcc ttaagtagaa gggacattct ttccggatca tgacaagtca atacaccaac
    14401 gtaaggcacc atttcagcta caggcacgcc gttggagaga agatccaatg cttcattata
    14461 atttttccct ttgaaaatta tttccatgta gaggtccgga tgtgatgctt taatctgcct
    14521 gaaactattg ctttccgata aagcttgcgc aaagtcgact tgttgtatgg caaagtttaa
    14581 ttcaacataa ccggtgattt tttcgatatc tatacccaga gaaatgattc tttcaaagaa
    14641 aagtcgatat agatcgattt ttttatttct gatcagatat cctgccagca ggttactatt
    14701 tcgtccaaac aataaattgc cttttttgat tacgatttcg ataatcctgt caatcaatgg
    14761 tgaggtgtga tccttttcga tgagaatttt aagaaaggat tcaaggccgg atggctcgtc
    14821 gagatattca gcataaaaag ggatgaggtt gaacgaatcg gttcgaatgt cgatctgaaa
    14881 taattgccac agccaattgc catcaaattc tccattcttt tttttgatcc ggttaatgat
    14941 tgtagtccgg aaaccctgaa tatcctgatt ttcatcactt aagccgatat cgatggcatt
    15001 gggagaaagg aaataattat aatgttcctt ctccaggagt gtgagcatga gtttctgaat
    15061 ctgccgctta agacctggcg caattggttg attgataata atggacagat tttgaaaagc
    15121 cttgctcaat agctgggttg cagaacgaac agagtgttca aaatccacct tgaaatactg
    15181 taataaaata ggctcaatga cctctacggc acccgtccag tccttggctg caagcatggt
    15241 gtcggtgcgg agaatatgca tttcgtatat ctgataaagc gcccggacgc ccttactgtc
    15301 caattttccg gctacattaa tgtaagaatg gctaatttcg gtgatataat cattgaatcc
    15361 tttaccgtat gaattgaact tataactaaa gaattgtttg aaaatcttac tgaatgaggg
    15421 attattggat gcataaaatc ttataaaact ctccatttcc tgcttctgta gaacagatag
    15481 caattcatca actcctaccc ttctacgtga gccgaccggg gttaatcgga cagacttatt
    15541 tctcaggtaa taaagtattc cggtaatatg tttacaacct ttttgctcat tgaatgaaag
    15601 gcagtcgcac tggaagttaa gaatcagtaa tggtgtttta ttgatacgag catgaatctg
    15661 aggtaaaccg tgatctgtat aagtagcgtt gatgcttcct ttctcttcag aaatgt
//
LOCUS       DAOQQN010000017        14958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1292_length_14958_cov_5.198752, whole genome shotgun sequence.
ACCESSION   DAOQQN010000017 DAOQQN010000000
VERSION     DAOQQN010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14958
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1292_length_14958_cov_5.198752"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /locus_tag="PLG24_01245"
     CDS             <1..1583
                     /locus_tag="PLG24_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ23535.1"
                     /translation="LKTFIAMAEGINTLPPQTSIPTAQYDLGYISLGESNYKEALGYF
                     EKAIAGFKTSRMQSDSYISGRLLPDATLRAGDCYLKNNNYTKAKEYYNIAINNRYPHF
                     VYALYQSAVISGLQGDRNGKIAGLRDISDKYRDSEYADDALLELANSYYTMNMMPEGI
                     AVLKRLVKDYKSKSDLINKALLKMGLMYYNSGDYTNALSNYKEVVRNNPTKEEADAAL
                     SAIKEIYVNDLKKPDEYFAYAETIPGYKATTDEKEKANFQVAEDYYEDGKYAEAIQAY
                     DEYLRKYPKGARIMDAVYHRGESYAAQKKYSEALTDYKAVIAMGSSVYYKDALEKSAI
                     ISYNYSNNFEDAYSYYTTWANIETKEDKRFEAQLGALRSAYRTGKKDEVIRLADLVTS
                     SSRASVDQKTSAHFYRAKAAQDLKDYTRAISSYNEVIRNTENEQAAESRYRIAEIYYL
                     NNDKESAEVMAQDAMEANGDYPYWVAKSLILTADILTDKGDYINARAALEAVIENFPD
                     DKEISAEAKRKLEQVKKK"
     gene            1655..3322
                     /locus_tag="PLG24_01250"
     CDS             1655..3322
                     /locus_tag="PLG24_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23536.1"
                     /translation="MKSHNRSRYFRLNNGLVALLFFATTGTVMSQNDLPSGKVEVIKS
                     YDAKLLPAEKLPLQGTIPEGSGDRKQYDYVINQDIKDKKPPVKTEAPDIRPMAVAAEK
                     LKDSYKGYFKAGIGYPLAGLVEGAYSVTNTKNYFIDIFGHHYGVYASPRENQVFAETN
                     VGVSGTNYLQSGLAMNGHVNFDLNNYRYYGGYNPGDTLFPSDPAKRRFTNIGAGLKIF
                     NSKKNTLDLDYWAGADMYGYKDNFVSTERGFSLDIGATKWIVDRHNFTLQITDHQLTY
                     GDTEKGHVNNVNVKPSFTFHGDKVKVKLGGNFVGSNGFAFFPDVEASANITGPAINVF
                     VGAYGDLYQNSFKRLSTINPFIYTRFAETGENGPFNTKYYDFYGGVGGGFNSVNYRLK
                     IGYKTNKDYALFVQQKEDLRQFDVIKDDVNIFYIGGELSASFFEKLNTSLHFLQQSAS
                     PRVQAKAYGIVPFEIGLNASYMMLEDKFKLKGDLNFAAGSPYRNRFDSVGNLNPMFDV
                     SIQGDYFFTDNFGAFLMVNNLASVKYQRWYRYPTYGINILGGISLRF"
     gene            complement(3385..3918)
                     /locus_tag="PLG24_01255"
     CDS             complement(3385..3918)
                     /locus_tag="PLG24_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23537.1"
                     /translation="MAKTEQPDSMPAKTVQINMDDDGSAKQEKTPVSRKDRLQQFVSR
                     AKTEIEKTANSYQPFTVEQARKLLATYASTASSTIRIILNERIADSKEENKLCFTVAS
                     NYEKENLLNEPGLLDYLRKNLNNQLITLEFEIDSSLMGDANDMKPISTEEKIKILVER
                     NALLQDFMEKLNLKIDE"
     gene            complement(3996..5147)
                     /gene="dnaX"
                     /locus_tag="PLG24_01260"
     CDS             complement(3996..5147)
                     /gene="dnaX"
                     /locus_tag="PLG24_01260"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HMZ23538.1"
                     /translation="MAQFIVSARKYRPQRFKDVLGQEHISHTLKMAFSQDKLAHAFLF
                     TGPRGVGKTTCARILAKALNCTGRTGDYEPCGKCESCISFDDNASFSIFELDAASNNS
                     VEHIRSLVEQVRIPPLAGKYKIFIIDEVHMLTTAAFNAFLKTLEEPPPYAVFILATTE
                     KHKILPTILSRCQIFDFRRIQVFDIVKQLSLVATEENIIAEEEAMHLIATKADGAMRD
                     ALSIFDRIAGATNNHITYQAVTNNLNLLDYTYFFKVTDQLITEDLPSVMLTIDEVLKK
                     GFDPEVFLSGLAEHLRQLLVSRDRATVKLIENTESVRERYAQQAALLPLDYILSALQL
                     CSECDINFRTAKNKRLHLEMTMIKMVYLPALIENGGINTPEKKKSLNPV"
     gene            5403..7037
                     /locus_tag="PLG24_01265"
     CDS             5403..7037
                     /locus_tag="PLG24_01265"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HMZ23539.1"
                     /translation="MKIALAQLNYIVGDFSGNHEKIIDNISMARQQGADLIVFAELAV
                     CGYPPEDLLEYRSFLQASEDAIRSLKLHSGDIGIIIGAPSLNHQRGKAYFNSAFFISE
                     GEVKEIVHKALLPEYDVLEEPRYFAPGRDFKVIEFRGKRIALTVCEDMWNIGNENPLY
                     DVCPMDMLIEQRPDFAVNISAVNFYIGKASLRKKVITSNAVKYKIPFFTVNHIGAQTG
                     LIFDGGSSVASPDGLIFREMPCFEEALEVFDLDEVIKGNHEDEVVMGEIELIHKALIV
                     GIQDYFKKQNLSSAILGLSGGLDSAVVAALATEALGKENVRGLLMPSQYSSSHSLEDA
                     ISLAQNLGIRFDIVPIKDIFDQYIFTLQPLFAGMPENVTEENLQARIRAMLLMAVSNK
                     YGNILLNTSNKSEVAVGYGTLYGDVCGAISVLGDVYKTEVYRLAHFINRDRIIVPLNT
                     INKAPSAELRPGQKDSDSLPPYEVLDQILYEHIENGKGSTEIVELGFDAQMVENILRK
                     VKFNEFKRRQAPPVLRVSRKAFGLGRKMPIVNGFSM"
     gene            complement(7090..7452)
                     /locus_tag="PLG24_01270"
     CDS             complement(7090..7452)
                     /locus_tag="PLG24_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23540.1"
                     /translation="MRFLALFFVVLFAFASCKKAEKTEAAPAMEAATEAPAVPQTANV
                     DVNAYLQEFNTLLTTYKEALAKKDTAMLSNLSMKYTELAQAGMEAAEKATGEDKEKML
                     SSIQMMSDQFKAISEGKK"
     gene            complement(7636..8040)
                     /locus_tag="PLG24_01275"
     CDS             complement(7636..8040)
                     /locus_tag="PLG24_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23541.1"
                     /translation="MKKIIICLFAAIALTSCKSDSKNETSATDTAATADAAAGKDMKD
                     SLILTQVPTFTDQATINFSAEYDSFLSEYRKAVRENNTEATQKLLDKYLDLAKKAAEI
                     SEKLNDEERSKFQQFITSRQAEYAEITSGDKF"
     gene            complement(8184..9167)
                     /locus_tag="PLG24_01280"
     CDS             complement(8184..9167)
                     /locus_tag="PLG24_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ23542.1"
                     /translation="MAKEAKGQFTEKLVDRLTVHFENDSYTLDEEYKAEINEFIRNLD
                     VVIPYVFRIQGHTDSIGSVQYNQRLSNRRSNTIKDYLLDLGIPEKLISLKGIGEMKPV
                     RPNSEEEGRAYNRRANIFVFEIQKYRLFRSKVILSGNKDNKAAVYIQTDEGMDSTFTD
                     DKGNFAFVIPENKNVTYGIFAKGYMFSTRTINTSRDLPIGDIVLNRIKPGEKVSLNNL
                     YFVGDQAILLPESQPELVNLTRFARNNPGLKLEIGGHVNGPKSYFGNPKWYYDLALNR
                     TKTIKEYLDAAGINTENYVCKSYSNTQMVNAEPKNEAEAKMNRRVEIKILE"
     gene            complement(9452..13084)
                     /locus_tag="PLG24_01285"
     CDS             complement(9452..13084)
                     /locus_tag="PLG24_01285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMZ23543.1"
                     /translation="MYRSMSGKGRQATILLSTAFLLLASIGIWTYVSQKNIEKRQIAD
                     IALRFQKLERKAQESIDNKEIIDFLSQWSEGTYLTDKASEKLITKLATSPVVLGMEAG
                     GRLTAWTGNIHSQETGKSLLNGYYLIDDSIFQKKYPIATGQDIKVVVEFGLPGKFYKE
                     KKDGLTELKSGDGNLTAYFLPSPKQKSLSGDWKILPFYLAAYTLLLIGLSKRLKRKTK
                     VPFIWSIGSTLLIAIAYRLLDWFLGAGMSIDIGLINSGLTPKPILSEWFANWILILLI
                     YFLVIENVIDNLPPIKASKRKRNPLLLYTTGLYALDYLGFVGILVFCRELVLRSGINF
                     DFSNVLNLGVGPLISLVLLALLLFRYFVFAYKINLVLYKNRIKLNNKLIGMIGGFLLM
                     LPWLLLLRLDLPLFILILVVLLFALLFDIFISTPSPSLGWLVIWIFFFSSFATIALFK
                     FNRDKDHDDRLALINILKNKEDSLLLRQFGEFVNNLKNDRQNNAAMTEALLQGSSISS
                     SNVIQNVLDEFEYIRQFYNVEFFVAQNDSLLQESIPGLKNNFVSQGVKFSEAGPYLIA
                     GRRKTFHGYWLKIYWQGYPQRPSPTITFSVTKKLYVPNTRDLDPRTVFLGSNLMRKYD
                     YAIYHGDNLVESQGYIYPDNLNTQKVFTRGGNMVETTSNNRSEIASKAGDGYVIIAGK
                     NLMGILKPVSLCSFLIVILLSIVISVAFLNNRFRILPAELPIFISGQFNLRQRIEYSI
                     ILVIVASFAIIAFVTGSYFNNLSLRMEESLLSEKAFGIVSDIESSLGNGGLDSIPEKS
                     LHMISANQQAGFSLYAIDGRERFNTIYAKIRSKKQNLMPPLPYLNLYFDNQSVYFDFA
                     GEKNTIRAVYVPVRKANNQKIGWLAVPYPEAGVTSIFAASDFLGTLLNVYVFLLLVAG
                     GLAFFVANSVTKPLVRLMDNLRKIKLGKKNEQLTWDQQDEIGSLIHEYNKMIDQLEES
                     TALLVKSEREGAWRDMAQQVAHEIKNPLTPMKLSIQYLERKIGSVPQEEMEQVVKDTA
                     RTIIEQIDNLASIATEFSNFAKMPAPIFEYINFNELVSSIHELFRKKDDIQFNLYVPI
                     DDVMVIADRAHLMRLLNNLVQNAIQSIPPGRQGRIGLELKIVDKNAVLSVKDNGQGIP
                     KDMHEKVFYPRFTTKSSGTGLGLAMCKSIVDSFNGTISFKTQPDVGTEFIVSIPLAQD
                     SHPEIP"
     gene            13547..14593
                     /locus_tag="PLG24_01290"
     CDS             13547..14593
                     /locus_tag="PLG24_01290"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HMZ23544.1"
                     /translation="MSESIQRAIEHFFPETGSFTLLPIKSGLINHTWKLTFDSSSFVL
                     QQVNKDVFTDPELIHGNLDLLRTYLARTVKDVHLVLPLSDQDDRSLFFDGESYYRIFP
                     WVEDSVTVSVVNSPEQAYEAARQFGRFTAAFHGFDHGLLKPVLPDFHNLRLRFDQFHH
                     ALKSGNPERIESSKEIIDQLLQKNRLVDIHDRMVASEQFRVRVMHHDAKISNVLFDRN
                     DKGICVIDLDTVMAGRIFSDWGDMVRTYVCPVSEEETELDRITIRKEYLEAIENAYLE
                     ELGDVMTEPEKKSLRISGMILIYMQALRFMTDHLHNDVYYGAAYPGHNFNRAVNQFML
                     LTALENSGDSTEWL"
     gene            14609..>14958
                     /locus_tag="PLG24_01295"
     CDS             14609..>14958
                     /locus_tag="PLG24_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HMZ23545.1"
                     /translation="MKKEAFREISPSIPREPGVYRFMDKDDNIIYVGKAKNLKNRLTS
                     YFNSTNKAGKTRIMVDHADHLEYTVVETEHDALLLEATFIKKFQPRYNVMLKDGKSYA
                     YICIRKEPFPRVFFT"
BASE COUNT         4176 a         2918 c         3394 g         4470 t
ORIGIN      
        1 agttgaagac ctttatagca atggctgagg ggattaatac cttgccgccc caaaccagca
       61 ttcctacagc ccagtatgat cttggctata tttcattggg tgaatccaat tataaggaag
      121 cactggggta ttttgaaaag gcaattgccg ggttcaagac atcaaggatg caatcagatt
      181 cttatatcag tggcaggttg ctgcctgatg ccacattgag agcaggtgac tgctatctga
      241 agaataacaa ctacaccaaa gccaaagagt attataacat tgcaatcaat aatcggtatc
      301 ctcactttgt atatgcacta taccagagtg ctgtgatctc aggtctgcag ggtgacagaa
      361 atggaaagat agcaggcttg cgggacattt ctgacaagta cagagattct gagtatgccg
      421 atgatgcctt gcttgaattg gccaattcct attatacgat gaatatgatg cctgaaggaa
      481 tagctgtatt gaagcgactt gtcaaagatt ataagagtaa gtcggatctg atcaataagg
      541 ctttgttgaa gatggggctt atgtattata attccggtga ttataccaat gcgctcagta
      601 actataagga ggtagtcaga aacaatccga ccaaagaaga ggcagatgct gcactttctg
      661 ccatcaaaga gatttatgtc aatgatttga agaaaccgga tgaatacttt gcctatgcag
      721 agacaatacc cggatataag gctactacgg atgaaaagga aaaagccaat ttccaggttg
      781 ctgaggatta ttatgaggat gggaaatacg ctgaagctat tcaggcttat gatgagtatt
      841 tgcggaaata tcccaaaggc gccagaatca tggatgccgt ctatcatcgg ggagaaagtt
      901 atgcagcgca aaagaaatat agtgaagcct tgacggatta taaggctgta atcgccatgg
      961 gaagtagtgt ctattataag gatgccctcg aaaaatcagc cataatcagc tacaattaca
     1021 gcaataattt tgaggatgca tatagctatt atactacatg ggcaaatatc gagacaaaag
     1081 aggacaagag attcgaggct caattgggtg cactacgaag tgcctacagg acaggaaaaa
     1141 aagatgaagt aatcaggttg gctgacctgg ttactagtag tagccgtgca tcagtggatc
     1201 agaaaacatc tgcacatttc tatcgcgcca aggcagctca ggatctgaag gattacaccc
     1261 gcgctatctc cagttacaat gaagtcatcc gcaatactga aaatgagcag gcagcagaat
     1321 caaggtatcg aattgccgaa atttattatc tgaacaatga caaggaatcg gctgaagtga
     1381 tggctcagga tgcaatggag gccaatggag attatccgta ttgggtggcc aaatccttaa
     1441 tccttactgc cgatatcctg actgacaagg gtgattatat caatgcaaga gctgcccttg
     1501 aagcagtgat tgaaaatttc ccggatgaca aggaaataag cgcggaggca aagagaaaat
     1561 tggaacaggt taagaaaaag taaagaagga tttatgatta tccttcatcg taatcatcgt
     1621 aatatttttt aaagaatcct agactgaaat taaaatgaaa tcccataata gatcaagata
     1681 cttcagatta aataatggtc ttgttgccct cctgtttttt gcgactacag gaacggtgat
     1741 gtctcaaaat gatttgccat ccggcaaggt ggaagtcatc aaaagttatg atgctaaatt
     1801 attgccagcc gaaaaactgc cattgcaagg tactataccg gaaggtagtg gtgatagaaa
     1861 gcaatatgat tatgttatta atcaggatat caaggacaaa aaacctccgg taaagacaga
     1921 ggcaccggat attcggccaa tggccgtggc agctgaaaag ctgaaggatt cctataaagg
     1981 atattttaag gcagggatag gatatccgtt ggcaggattg gtggagggtg catattccgt
     2041 gaccaatacc aagaattatt tcatcgatat ttttggtcat cattacgggg tttatgcatc
     2101 gccaagagaa aaccaggttt ttgccgaaac aaatgtcggt gtaagcggga ctaattatct
     2161 tcaatccggg cttgcaatga atggccatgt caactttgac ctcaataatt accgttatta
     2221 cgggggctat aatccgggcg ataccctatt cccgagtgat ccggcaaaaa ggaggtttac
     2281 caatattggt gctggactga aaatcttcaa tagcaagaaa aatacacttg atcttgacta
     2341 ttgggccgga gcggacatgt atggctataa ggataacttt gtatcgacgg agcgagggtt
     2401 ttcacttgat atcggtgcaa caaagtggat tgtggacagg cacaacttca cccttcagat
     2461 tacagatcat caattgacat acggagatac tgaaaaaggc catgtgaata atgtgaatgt
     2521 caaaccttcc tttacctttc atggggataa ggtcaaggtg aagctcggtg gaaactttgt
     2581 agggagcaat ggatttgcat tcttcccgga tgttgaagcc agtgccaaca taaccggacc
     2641 ggccatcaat gtttttgtcg gtgcttatgg cgatctttat caaaacagct tcaaacgatt
     2701 aagtaccatc aatccattta tttatacacg atttgcagag accggtgaga atggaccttt
     2761 caataccaag tattatgatt tttacggtgg tgtaggtggc ggcttcaact cggtgaatta
     2821 tcgactgaag attggctaca aaaccaataa ggactatgcg ctttttgttc agcaaaagga
     2881 agatttgagg cagtttgatg tcatcaaaga tgatgtaaat attttctaca tcggtggcga
     2941 gttgtctgca tccttctttg aaaaattaaa tacttccctc catttcctgc aacagtccgc
     3001 atctcctcgt gtccaggcaa aggcctatgg aattgttcca tttgagatcg gcctcaatgc
     3061 ttcttatatg atgctggagg acaagtttaa acttaaaggt gatttgaatt ttgcggccgg
     3121 aagtccatac aggaatagat ttgattcggt aggcaacctt aatcctatgt ttgatgtcag
     3181 tattcaaggc gattatttct ttacggacaa tttcggagcc ttcctgatgg tcaataatct
     3241 ggcatctgtc aagtatcagc gctggtaccg atatccaacc tatggtatca acatattggg
     3301 tggcattagt ctgagatttt gatggttaat attacttcag ccctgcacaa cggatgtgtc
     3361 ggtgggtcag gtagacaagg ggaattactc gtcgattttg aggttgagtt tttccatgaa
     3421 atcttgcagt agggcattcc tttccactag aatttttatt ttttcctcgg tggagatagg
     3481 cttcatatcg ttggcatctc ccatcaaaga ggaatcaatc tcgaattcga gtgttataag
     3541 ttgattatta agatttttcc tcaaataatc caaaagcccc ggttcattga gtaggttttc
     3601 cttttcgtag ttggaagcta ctgtaaagca gagcttgttt tcttccttac tatccgcaat
     3661 tctttcgttt aggatgattc ggatggtgga cgatgcggtt gaggcatagg ttgccaatag
     3721 ctttcgtgcc tgctcaacgg taaacggctg ataagagttg gctgttttct ctatttctgt
     3781 tttggcccgg gaaacgaact gctgcaatct gtcttttctg gaaacaggcg ttttctcctg
     3841 tttggctgat ccatcatcat ccatattgat ttgaacggtt ttagccggca tagaatccgg
     3901 ttgttccgtt tttgccatac cataattttc ttcaatggta cttgatgata ttggtgggac
     3961 gattgaggta ttttgttccg gtagggtagt ttttatcaga ccgggttcag ggactttttt
     4021 ttttcagggg tgtttattcc tccattttca atcaaggcag gcaaatagac catttttatc
     4081 atggtcattt ccagatgcag acgtttgttt tttgctgttc tgaaattaat atcgcactca
     4141 ctgcatagct gaagtgccga caggatataa tcaagaggta ataaagctgc ttgttgtgcg
     4201 tacctttccc gtaccgattc ggtgttttca ataagcttga cagttgcccg gtcccgactg
     4261 acaagcaatt gccttagatg ttctgccaga ccggataaga atacttccgg atcgaaccct
     4321 tttttcaaga cctcatcgat agttagcatg accgagggca gatcttcagt aatcaactga
     4381 tctgtcactt tgaaaaagta tgtataatca aggaggttca gattgttggt gactgcctgg
     4441 taagtgatat gattgttggt tgctccggct attctgtcga agattgaaag cgcatccctc
     4501 atggctccat cggcctttgt tgctatgaga tgcatggcct cttcttcagc tataatgttt
     4561 tcctctgtgg caacaaggct caactgtttt acgatatcga acacctggat gcgccgaaag
     4621 tcaaaaatct ggcatcttga taatatggta ggcaggattt tgtgcttctc cgtggttgcc
     4681 aggataaata ctgcataggg aggtggctct tccagcgtct tgaggaaggc attgaaggct
     4741 gcagtggtca gcatatgtac ctcatcgatg atgaagatct tgtatttacc tgccaacggt
     4801 ggaattctga cttgttctac caggcttctg atgtgttcca cactgttgtt ggacgctgca
     4861 tctaactcaa agatcgagaa ggaggcgttg tcgtcaaatg aaatgcatga ttcgcatttg
     4921 ccacatggct cataatcacc tgtcctcccg gtacaattta atgctttggc aagaatacgg
     4981 gcacaggtcg tcttgccgac cccccggggc ccggtgaata gaaaggcgtg agcaagctta
     5041 tcctgcgaaa acgccatctt cagggtatgg gagatgtgtt cctgtcccag gacatccttg
     5101 aagcgctgtg gcctatactt tcttgctgaa acgataaatt gtgccataaa tggagggtga
     5161 aagtatatag tttatttgaa aattatgcca aatctgactg attatttttc ggaatattat
     5221 agaatgtcgg atagcaaata gattgttcat ttgggttatc cgactatttc actgcaacag
     5281 gcagttaaaa aaaacacaga tgcatcaaat gtttttcttt taaataaacg aggtagactt
     5341 aactttgttt accgattctg ctattcctta gcaaatcggt ctctactgaa taattattaa
     5401 gaatgaaaat agcattagcg caattaaatt acatagtcgg agatttttcc ggcaaccatg
     5461 aaaagattat tgacaatatc tcaatggcca ggcaacaggg cgcagatctt atcgtatttg
     5521 ccgaattggc agtatgcggt tatccgccag aggatcttct ggaataccgg tcgtttcttc
     5581 aagccagtga agacgccatc cggtccttga aactgcattc cggagatatc ggaatcataa
     5641 taggcgcacc ctctctcaac catcaaagag ggaaagccta cttcaatagt gcatttttta
     5701 tcagtgaagg tgaagttaag gaaatagtcc ataaggcgtt gttgcctgaa tacgatgttt
     5761 tggaggagcc gagatatttt gccccgggaa gggattttaa agtaattgaa ttccgcggca
     5821 aaagaattgc cctgaccgta tgtgaagaca tgtggaacat aggtaatgag aatcctttgt
     5881 acgatgtctg cccgatggac atgctcatcg aacagcgtcc tgactttgct gtcaatataa
     5941 gcgcggtcaa tttttacatt gggaaggcaa gtcttcgcaa gaaggtgatt acctcaaatg
     6001 ctgtaaaata taaaattcca ttttttaccg taaaccacat tggtgctcag accgggctga
     6061 tatttgacgg aggctcatct gtggcctcac cggatggttt gattttccgg gaaatgccct
     6121 gttttgaaga ggcgcttgaa gttttcgatt tggatgaagt aataaagggt aatcatgagg
     6181 atgaggtggt aatgggtgaa attgagttga tacataaggc tttgatagtt ggaattcagg
     6241 attatttcaa aaaacagaat ttatcttcag ctattttagg tctgtcggga ggattggaca
     6301 gtgcagttgt tgcagcgttg gcgactgagg cgttggggaa ggagaacgtc agagggctgc
     6361 taatgccttc acagtatagc agcagccaca gtttagaaga tgctatttca ttagctcaaa
     6421 atctcggtat caggtttgat attgtcccga taaaggatat tttcgatcaa tatattttta
     6481 cgttacaacc tttgtttgca ggcatgccgg aaaatgtgac cgaggaaaac cttcaagcgc
     6541 gcatcagagc tatgttgctc atggcagtca gtaataagta tggaaatatc ttattgaata
     6601 caagcaataa aagtgaggtg gcagttggct atggaacact ttacggcgat gtgtgtgggg
     6661 cgatttcagt gcttggtgat gtttataaaa cagaagtgta tcgcctggcg cattttatta
     6721 acagggatag gatcatagtg ccattgaaca ctatcaacaa ggctccttca gcggaattgc
     6781 gacccggaca aaaggatagt gatagtctgc ctccttatga agttctggat caaatccttt
     6841 atgagcatat tgaaaatggc aaggggagta ctgagattgt ggaattgggt tttgatgctc
     6901 agatggttga aaatattcta agaaaagtca aatttaatga gttcaagcgg cgtcaggctc
     6961 cacctgtatt gagggtatca agaaaggcat tcggtctggg caggaagatg cccattgtca
     7021 atgggttttc gatgtaacaa aacagcccga agaaatctcc gggctgaatt ttatttgctt
     7081 taacgaaaat tacttttttc cttctgaaat agccttaaat tgatctgaca tcatttgaat
     7141 ggaggaaagc attttctctt tatcctctcc tgttgccttt tcagcagcct ccatgccggc
     7201 ctgggctagt tcagtatatt tcatggaaag attgctgagc atggcagtgt ctttctttgc
     7261 caaagcttcc ttgtatgttg tcagcaacgt gttaaattcc tgcaagtatg cattgacgtc
     7321 cacattagct gtttgaggta cagcaggggc ttctgttgca gcttccattg ccggtgctgc
     7381 ttcggttttt tctgcttttt tacaacttgc aaaagcaaaa agtactacaa aaaataaagc
     7441 taagaatctc attggtaatt aattataaac tattaataaa aaaatatctt ccctaacatg
     7501 acatcgtccc gaagatgatt gaacatatta ttgagcatta tgccggtgtg tttcaatcat
     7561 ggagacggtt gtgtattcat ggaaaaagtt aaacatatag ggcaatttgc tttccgttgg
     7621 tttatctttt gaatatcaga atttgtctcc tgaagtgatt tcagcatatt cggcctgtcg
     7681 tgatgttatg aattgctgga atttacttct ctcctcatca ttcagttttt cagatatttc
     7741 agcagctttc ttagccaggt caaggtattt gtccaataac ttttgagtag cttccgtatt
     7801 gttttctctt acagctttac gatattcact tagaaacgaa tcatattcgg ctgaaaagtt
     7861 gatggttgcc tgatcagtaa aggtcggcac ctgagtaagg atgagggagt ccttcatgtc
     7921 ctttcctgca gctgcatcag cagtagctgc tgtgtcagtg gctgaagttt catttttaga
     7981 gtcactcttg cagctggtta aggcgatggc agcaaacaaa caaattatta tttttttcat
     8041 attatacttt acattttggg gccaaagata acattttgct ataaatagca gtacagcgag
     8101 ccaaccgaat tttgacggta ctttttgaaa acggtcacat caatcgagca ttttttatgt
     8161 cttgattccg gattttttat ctcctattcc agaattttta tctccacacg tctgttcatc
     8221 ttagcctccg cttcgttttt gggttctgca tttaccattt gcgtgttgga ataggatttg
     8281 catacatagt tctctgtatt gatgcctgct gcatccagat attccttgat ggttttcgta
     8341 cgatttaacg ccaggtcata ataccatttc ggattgccaa aatatgattt aggcccgttg
     8401 acgtgacctc cgatttccag tttgagaccc ggattatttc tggcaaatcg tgtcagattg
     8461 accagttccg gttgtgattc gggaagaaga atggcctgat cgcccacaaa ataaagattg
     8521 ttgagtgata ctttttctcc gggtttgatc ctgtttaaga caatgtctcc tatcggcaag
     8581 tccctggaag tattgatcgt tctggttgaa aacatataac ctttggcaaa gataccatag
     8641 gttacatttt tattttcagg tatgacaaag gcaaagttcc ccttgtcgtc agtaaaagtg
     8701 gagtccatac cctcatcggt ttgaatatag actgctgctt tgttgtcttt attcccgcta
     8761 agaatgactt tactacggaa tagtcgatat ttttgaattt cgaagacaaa aatatttgct
     8821 cttctgttgt aggccctgcc ttcctcctcg ctgttgggtc ttacgggctt catttctccg
     8881 attcccttca gggaaatcaa cttctccgga ataccaagat caagaagata atccttgatg
     8941 gtgttggatc ttcggttaga aagcctttga ttgtattgta cgctgccgat tgaatcggta
     9001 tggccttgaa tcctgaagac ataagggatg accacatcca gattgcggat aaattcattg
     9061 atttccgctt tgtattcttc atcaagcgta taggaatcat tttcaaaatg cacggtcagg
     9121 cggtcaacaa gtttttccgt aaactgaccc tttgcttctt ttgccaatat tgttagtaaa
     9181 atgacgggaa tgatcctgaa taaatgctta ttaaaaaaag ataacattgc aacaaacaat
     9241 aaggtgttca gaataaaaat tactaaggat agtttgaaaa tcaaccatct tctattttat
     9301 taattggaca aaaatttcgt gcgcaaacag gtagattgac ctacttaatc ctaaagatat
     9361 aaaaaatatt gggatccggt attttgccgg gagatatttt gttgttacgg gtttgaatgg
     9421 tgtaacgata aaggttttag gtgattctgt tttagggaat ttccgggtgg ctgtcctgag
     9481 ccaatggaat gctgacaata aattctgtac cgacatcagg ttgagttttg aagctaatgg
     9541 tgccattgaa tgagtctacg attgatttac acatagccag acccaggccc gttccactgc
     9601 tcttcgtggt aaagcgggga taaaatacct tttcgtgcat atctttagga attccctgac
     9661 cattatcttt tacggagaga actgcatttt tgtctacaat cttcaactcc agtccaatcc
     9721 ttccctgcct gccgggggga atgctttgga tggcattttg gacgagattg ttgagaagtc
     9781 tcatcagatg ggctctgtca gcaattacca tgacgtcatc tataggcacg tagaggttaa
     9841 actggatatc atccttcttt cggaataatt catggatgga agacaccagt tcattgaagt
     9901 tgatgtattc aaagattggg gcaggcatct tggcaaaatt ggaaaattct gttgcgatac
     9961 ttgcaagatt gtcgatctgc tcgatgatgg ttcttgctgt atccttgacg acttgctcca
    10021 tttcttcctg cggtacgctg cctatctttc gctcaagata ttggatgctc agcttcatcg
    10081 gggtaagcgg attttttatt tcatgtgcga cctgttgggc catgtcgcgc catgcacctt
    10141 cgcgttcgct tttgaccaac agtgccgtgc tttcttccag ttgatcgatc attttgttgt
    10201 attcatgaat cagggatcct atttcgtcct gctgatccca ggtcagttgc tcattctttt
    10261 tacctaattt tattttcctg agattgtcca ttagtctgac gaggggcttg gtcaccgaat
    10321 tggcgacaaa aaaggcaagg ccgcctgcta caagaagtaa gaagacatac acattgagta
    10381 aggtgcccag gaagtcagaa gcggcaaata tagaagtaac gcctgcttcc ggatacggaa
    10441 ccgcaagcca gccgattttt tgattatttg ccttacggac aggtacatat acggcacgga
    10501 tagtattttt ttctccggca aagtcaaagt acacggattg attatcgaaa tatagattaa
    10561 gatagggtaa gggaggcatc aggttttgtt ttttactcct aatctttgca tatatggtgt
    10621 taaaacgctc ccttccatcg atggcgtaaa ggctgaagcc ggcttgttga ttggctgaga
    10681 tcatgtggag cgacttctcc ggaatcgaat caaggccgcc attccctaag gatgattcta
    10741 tgtctgatac aatgccaaat gccttttcgc tcaacaggct ttcttccatc ctcaatgaaa
    10801 gattgttgaa ataacttccg gtcacaaatg cgatgattgc aaaggaagct acaatgacca
    10861 ggatgatgct gtattctatt cgctggcgca gattgaattg accggagatg aatattggta
    10921 gttctgccgg aagtattctg aatctgttgt tgaggaatgc tacactgatg acgattgaca
    10981 acaggatgac aattaaaaaa gaacaaagtg agacaggttt taggattccc atcaggttct
    11041 tgcctgcgat gatgacataa ccgtctcccg ccttcgatgc aatttctgat cggttgttgg
    11101 aggtggtttc gaccatattg ccacccctgg taaaaacttt ctgtgtattc aggttgtcag
    11161 ggtaaatgta gccttgactc tcaaccagat tgtccccatg atagatggca tagtcatatt
    11221 ttctcatcag gttcgagccc aggaatacgg tgcggggatc caaatccctg gtattgggaa
    11281 catacagctt cttagtaacg gaaaatgtta tagtaggtga tggtcgctgg ggataccctt
    11341 gccaatagat cttcagccag tatccatgaa aggtctttct ccggccggct ataaggtaag
    11401 gtccggcttc actgaatttg actccctgac ttacaaaatt gtttttcaag cccggtatgg
    11461 actcctgtaa tagagaatca ttttgtgcaa caaaaaactc aacattgtaa aattggcgga
    11521 tatattcaaa ttcatccaac acattctgga taacattgct ggatgaaatg gagctgcctt
    11581 gcagcaacgc ttcagtcata gcagcattgt tctgcctgtc atttttcaag ttgttgacaa
    11641 attctccgaa ctgacgtaat aataagctat cctccttatt ttttaaaatg ttgatcagcg
    11701 cgagcctgtc atcatgatcc ttgtcccgat tgaatttgaa gagggcaatg gtggcaaagg
    11761 acgaaaagaa gaatatccag atgaccagcc agcccaatga tggcgagggt gtgctgatga
    11821 agatatcgaa tagtaaggca aaaagcaata ctaccaatat gaggataaaa agaggcaaat
    11881 ccagacggag taaaagcagc cagggaagca tgagcagaaa gccgcctatc atgccaatga
    11941 gcttgttgtt gagtttgatc ctgtttttat acaggacaag gttgatctta taggcgaaaa
    12001 cgaaatacct gaatagcaac aaagccagca atacaagact tatcaaaggc ccaacaccca
    12061 gatttagcac attactgaag tcaaaattga ttccgctgcg caaaaccaat tcccggcaaa
    12121 agaccaatat gcctacaaag cctagatagt cgagtgcgta aagtccggta gtgtacagaa
    12181 ggaggggatt tcttttcctt ttgcttgctt tgatgggtgg caaattgtca atgacgtttt
    12241 caatgaccag gaagtagatc aatagaatca gtatccaatt ggcaaaccat tcgctcagta
    12301 tgggtttggg tgtgagaccc gagttgatca gtcctatatc tatggacatg cctgctccta
    12361 aaaaccaatc cagcaacctg taagcaatgg ctatcaaaag agtagatccg attgaccaga
    12421 taaatggtac tttcgttttc cttttcagtc tcttggatag accgatcagc aacagggtat
    12481 aggctgcaag ataaaaaggc agtatcttcc agtcaccact gagacttttt tgtttgggag
    12541 atggcaaaaa ataggcggtc aggttgccat cacccgattt taattctgta agcccatcct
    12601 tcttctcttt gtaaaacttt cccggtaacc caaactcaac aactaccttg atatcctgtc
    12661 ctgtggcgat cgggtatttc ttttggaata tggaatcatc aatcaggtaa tacccattga
    12721 gtagggactt tccggtttct tgactatgaa tgttgcctgt ccatgcggtg agtctgccac
    12781 cggcctccat gccgagtaca acaggcgaag tggccagctt ggtaatcagt ttttcagatg
    12841 ccttatccgt taggtaggtg ccctctgacc attggctcag gaagtcgatt atctccttgt
    12901 tatcaatgct ttcttgtgcc tttctttcta gtttttggaa ccggagagca atatcagcaa
    12961 tttgccgctt ttcgatattc ttttgagaaa catatgtcca aatgccgatt gatgccagca
    13021 ataaaaacgc tgtacttaat aagattgtag cttgtcttcc tttgccagac atggatctgt
    13081 acatcaaatt gttagtaaaa gcccattgac ctgcttatgc atggttcttt tttctgaaac
    13141 ctcaaatgcg gtggtattga atcatcgtca tgccccagct tttcgtttag tgaagtgttg
    13201 gatcatgatt caaccctttt gttgcaatac ataactgcct cgcaatgcgc ttgcaaggta
    13261 caaaatatat cgatatatat tataatttta tttaatcttt ggtagtggtg tcatcggagt
    13321 gattacaaaa ggacgggcct gacacattgt ttttcttact ttgatttccc ggttacaaaa
    13381 tcgaccctgt taagtgactt ctggggaacc gaattataaa tttaataaag ccggcgtatc
    13441 cgggtagcgg tgtcaaagac aagagtaatt gaaaagggct ttttattttt taatccggaa
    13501 ctttcgataa ttgtattatt tttattttga caacagactg taatttatgt cagaatccat
    13561 ccagcgtgca atcgaacatt tctttccgga aacaggcagt ttcactttat tacctatcaa
    13621 gtcaggcttg attaatcata catggaaact tacctttgac tcttcttcct tcgtccttca
    13681 acaggtcaac aaggatgttt ttactgatcc tgaattgatt catggtaatt tagacctact
    13741 gagaacttat ttggcaagga ctgttaagga cgtccacctg gtcctgcctt tgtctgatca
    13801 ggatgaccgt tccctttttt ttgacggaga aagttattac agaatctttc cttgggtgga
    13861 ggacagtgtg actgtgtcag tcgtcaattc tccggaacag gcatacgaag cagctaggca
    13921 gtttgggagg tttacggcag cattccatgg atttgatcat ggcttattga agcccgtcct
    13981 tccggatttt cacaatctaa ggttgcgttt tgatcaattc catcatgcac ttaagtcagg
    14041 caatccggaa agaatcgaat cttcgaagga aattatcgat caattgcttc agaagaatcg
    14101 attggtagat atccacgatc ggatggttgc atccgaacag ttcagagtgc gcgttatgca
    14161 ccacgatgcc aagatcagca atgtgctttt cgacagaaat gacaagggta tatgcgtcat
    14221 cgatctggac accgtgatgg ccggacgcat tttcagcgac tggggagata tggtgcgtac
    14281 ctatgtatgt ccggtgtcag aagaggaaac cgagttggac aggatcacga taagaaagga
    14341 atatcttgag gctattgaaa atgcctatct cgaggaactc ggcgatgtca tgactgagcc
    14401 cgaaaaaaaa tccctgagga tttcaggaat gatattgata tacatgcagg cattgcgatt
    14461 catgacggat cacctgcaca atgatgtcta ttacggtgcg gcttatccgg gtcacaattt
    14521 taaccgcgca gtcaatcaat tcatgctcct gactgcattg gaaaattcag gcgattcaac
    14581 tgagtggctc taaatttgtt tgacaaggat gaagaaagaa gcattcaggg aaatatctcc
    14641 ttctattccg agagaacccg gagtctacag gtttatggac aaggatgaca acatcattta
    14701 tgtcggcaag gctaaaaatc tgaaaaacag acttacctcc tatttcaatt ccaccaataa
    14761 agccggaaag acgcggatca tggtagatca tgcggaccat ctggaatata cggttgtcga
    14821 gacggagcac gatgcattgc tgctggaggc cacgtttatc aagaagtttc aaccacggta
    14881 caatgtcatg ctcaaggatg gcaagagcta cgcctatata tgtatcagga aagagccttt
    14941 tccgagggtt ttctttac
//
LOCUS       DAOQQN010000018        14810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1318_length_14810_cov_5.303287, whole genome shotgun sequence.
ACCESSION   DAOQQN010000018 DAOQQN010000000
VERSION     DAOQQN010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14810
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1318_length_14810_cov_5.303287"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..2273
                     /locus_tag="PLG24_01300"
     CDS             246..2273
                     /locus_tag="PLG24_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HMZ23546.1"
                     /translation="MKKHVHIYDAAGNQLCCTLEQKINQKSDVTLVAAEPACCNHDHA
                     DNHVEEHDHIASVDGGWWHLFRPAIISFVLLISGIILDHYEPSFYDQWVRIGLYLLAY
                     IPVGWPVLKEAWESIAKGEVFSEFMLMGIATLGAMGIEQYPEAVAVMLFYSIGETFQT
                     LAVQRAKANIKMMLDQRPDEVTIVNGDKFETVKAATVPPGTIVRLNPGEKAALDGILL
                     SASGSFNTAALTGESVPATKFKGEEILAGMISVNESCRMEVSRPYEDSKLSGILRMVQ
                     DASSRKAPTELFIRKFAKVYTPIVVFLAVAICLLPFFFVSDYIFSQWLYRALIFLVIS
                     CPCALVISIPLGYFGGIGAASRNGILVKGGNYLDTLAGIRHVVVDKTGTMTRGNFEVR
                     EVEISEGFNRIEVLTDLKALQGFSTHPIAGAIQRYVQEEVVGIRVEDVLEIAGKGMSG
                     TVNGKHLLAGNLALMQVNKIPYDVSLEEIEYTLVAIAVDGKFAGYVTIADEIKPNAAA
                     VVRELADMGVELTMLSGDSNKVVAAVAREIGIRKAYGGLLPEDKVAKANEIKEASPPI
                     AFVGDGMNDAPVLALSDVGIAMGGLGSDMAIESADVVIQDDDIKKIPLSIRIGKKTKT
                     IVWQNILFAFGVKAAVLILGASGEATMWEAVFADVGVALIAVANAVRIQRM"
     gene            complement(2457..5024)
                     /locus_tag="PLG24_01305"
     CDS             complement(2457..5024)
                     /locus_tag="PLG24_01305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HMZ23547.1"
                     /translation="MLYIFFFVLSFYKNTLFILIMEENMERIKSIVRKMWIGLGVLVL
                     FGFAYIIFLSLSGLPTFEELENPKYDFASDIIASDNSVLGRLYIENRVPITYSQISPN
                     IVKALIATEDVRFEKHSGVDPEAVARVIAKTVLLARKSSGGGSTITQQLAKLLYSDRD
                     FSNMGRIRKSFALVNIKLKEWITAIKLERRYTKEEIIAMYLNKFNFIYGAYGIEAAAE
                     TYFSKNNKDLTISEAAVLVGMLKNPSLYNPVRRREQATLRRNTVLKRMYSNGILSESD
                     YEKLSAEPIQLDFKPKTHIDGDATYFRMEVGKEVSQIIRKMDLQKSDGSLYDIYRDGL
                     KIYTSIDADMQRIAEQVMLKHMKTVQTNFWREWKGKDPWKYNADAKQLAIREASMKNL
                     VRSSDRYINMRQNIFEQELDEINAKYPEISFSDIEFDLWTAAEKEGLDKTAKRYRITD
                     EQKRVYEQIMADPEYKITVAKWQQFRSTVRKAFNEKYKMKVFAYNAEGQKDTVMTPYD
                     SIRYHRMFLQTGIVAIEPTTGQVKVWVGGINHKYFKFDHIRTKRQVGSTFKPFVYASA
                     IAFRGISPCMRVVDQQYTIEPGEASFGLIKPWSPGNAEGKFSGKSMTLYEALRESRNS
                     VSVYLMKQLQSTDQVLELVNNMGIDKSKIPAQPSICLGSADLTVLEMTGAYASFANNG
                     TYVVPSFISRIEDKNGKVIYKNASDEKQALAPDYNYVMVDLLRYATRGSAGFQGIKSE
                     VGGKTGTTNDYVDGWFMGITPSLVVGTWVGGDDRWIHFNSLTYGQGSKMARPFFSEFI
                     RQLELQKVSDYDPNARFIVPAGSENIVTDCSQYSNPNVIDQPIDTVKRKPGEDDFFQ"
     gene            complement(5076..6848)
                     /locus_tag="PLG24_01310"
     CDS             complement(5076..6848)
                     /locus_tag="PLG24_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMZ23548.1"
                     /translation="MGQLTLQRPEYLLLLLVVPILGISMSLWRKRRRIIAADFAGKEV
                     YQKIAEPPKDNYRTRITLMLTALILAILALAGPSLPGPPEKTIKRASDIFLAIDVSRS
                     MQAQDLAPDRLTKTKLWAAELVQILKGQQIGLVVFAGAAYMYMPLTDDYNAAIDFING
                     ITTEMASYQGTAIGDAIETAQKAFGDETNKSRGLVIISDGEDHDSNAIQAAEKAAKKG
                     IVITTIGAGTEKGALIPEQDDLGFKYKLDEEGQPVRSKLNKEELIKIAAAAHGRYYSL
                     DNPRSALEGVKNMVDNLDKGTYDVHETSNNISLFPWLLWPAFFLFLIEMIMSMNISIK
                     TKSNNRTKVTTVTALMVFLLHNASYGQSAHTIMKEGNIAYQAKDYKKAAEHYDAAAKK
                     KPDFNSLYNEANALYQLGKYPDAAEKNMEAYQYAKTPQQKNMVTYNLGNSFFQTKDYE
                     KAIDAYKQALRNDPGDLDAKANLTRALVEKNKQDEQKKNNQKNQDKNQDQRQDQDQKD
                     NQNQNNQNQNNQNQQKNEPGNQAQNANQSNRQDKQNNQGKPQHLSPQEAESLLRIMKE
                     EEKNTRGKVMKRQQPEYNRSGKDW"
     gene            complement(6849..7859)
                     /locus_tag="PLG24_01315"
     CDS             complement(6849..7859)
                     /locus_tag="PLG24_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMZ23549.1"
                     /translation="MLELGNIAFRFAWVLLFIPLLWWMFIILRRQRRTSGVYLPTAAA
                     VKDFVTLRTKLFRYLPWIKFAGLSFILISLAGPQRLEKNETINDSGIDISLVIDLSSS
                     MLARDFKPNRLEVTKTLAEQFVKERPNDRISVSVFAGEALAQCPLTYDHRMVVNILRN
                     LNAGALDDGTAIGMGLVSAVNTLKDSKAKSKVVILLTDGVNTAGYIDPLTAAKLAADY
                     HLRVYVIGVGSEGEAETPISRTPSGQVIYGYVPVEIDEPLMTEIANLTNGQYFRAKDE
                     NSLQQIYNRINLLEKTKIEKTVLKKRYDEYAWFLLIGIGLILTETILKNTWVRTPLFI
                     KY"
     gene            complement(7852..8721)
                     /locus_tag="PLG24_01320"
     CDS             complement(7852..8721)
                     /locus_tag="PLG24_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23550.1"
                     /translation="MEVTADTNSILLGDRIKLTFTITCDPSISIKKLVLDSITTIPGF
                     ELSDEKEWVERKTQMSKILTKEMMFTAFEPGDYEFPAVPFFYDYQNTEKKGFSRPWRL
                     KVLPMLNKSEDIAPNKDIVVVITFWDRFGVYIIISLAVILLGVLSALLVSYLKRRSKY
                     TPQAPVMQISPAQKARKELALLRKNELWKAEDHKMFQTKLSLILRIYLQEAFDIPALK
                     STSHEIVSQLKKTGLPYNVIDTTDKALNIADLVKFAKAQSREEINFSFIGKVETLIDE
                     AESFKGKIPDRYA"
     gene            complement(8799..9674)
                     /locus_tag="PLG24_01325"
     CDS             complement(8799..9674)
                     /locus_tag="PLG24_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HMZ23551.1"
                     /translation="MEVNELLRKVRKVELKSRDMSRLLFSGDTLSFFKGRGMSFAEVR
                     EYQYSDDVRFIDWNVTARTGVPFVKVYEEERELNVIIMVDVSKSSFFGTGTRLKSELI
                     TEMSAVLAFSAVQNNDRVGLILFSDKIELYIPPSKGRNQILRIIREMVNFRPENKNTD
                     IRKALRFLNTVIKKNSICFMLSDFQDDLFGAQMRIAGRKHDLIAIHIEDKAEHQLPDV
                     GMLIAVDPETNEEFVLDTSDPAFRTAYQKQFEARKKKLQKIFGESKVDQIEITMGEDE
                     NFVRKLLYFFKSRAR"
     gene            complement(9674..10966)
                     /locus_tag="PLG24_01330"
     CDS             complement(9674..10966)
                     /locus_tag="PLG24_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23552.1"
                     /translation="MRFLVILFCAFICLPGYAQLTSCKDLWEGEASDRQYVISGWVDG
                     THTFRMFLCRKGDEVRGYYEMISAKKNTDVEGFFKGDSIFLAEYSKNQKVLGMIRGIF
                     AGTVFTADLSSPSGVYSGKMEGLTVGSWVEAKTPEPIDNPAIVVFTGVANPEETIVLS
                     FPNDYSCKGILHNTDLKKTYHMSGKRPDNTERKYNLKSYSLPQDGANMTCILNDEILI
                     QSTLPGKTSAMSAGIKIPLALIEKSDRLVSFEIHYPVIGTKGKENKLNIMVNDIRYEF
                     DSLFDIHLKNIHGEERPYSRAGTMVAWFEPSYVSGQWLCGHFIIRNNERNTSHIMPVN
                     YNYKKNIMVNIEDLISSPNDIGKQWNSIIPKQPEASDFSAKDASYGAPVLAFRGIVLS
                     TEYDPIFDRQLFQTNKKIKGVRWKWWKPEYWMIKFQQF"
     gene            complement(10994..11992)
                     /locus_tag="PLG24_01335"
     CDS             complement(10994..11992)
                     /locus_tag="PLG24_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HMZ23553.1"
                     /translation="MTQYSEIEALNERVYKSSEFVSHLSDATANIIVGQEHMIERLLI
                     GLITKGHILLEGLPGLAKTLAIKTLATLVNGKFSRIQFTPDLLPADIVGTMIYNPTLN
                     KFSVKKGPIFANFILADEINRAPAKVQSALLEAMQERQVTIGEETFALEEPFLVMATQ
                     NPIEQEGTYPLPEAQLDRFMLKVTIDYPTKEEEQHIIRRHMDDSIGQLQQPVATTQEI
                     LDARAIIKSIYLDPSLERYILDIVFATRDVKSAGLPDLAPMIQYGASPRASINLALAS
                     KALAFIRRRAYVIPDDVRAIAMDVLRHRIGITYEAEAEGLTGESIITSILNIVNVP"
     gene            complement(12367..12489)
                     /locus_tag="PLG24_01340"
     CDS             complement(12367..12489)
                     /locus_tag="PLG24_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23554.1"
                     /translation="MKPVAKEVKVVLHEMFKDVRIWAIMLIVLFLLIFITRFPV"
     gene            12756..>14810
                     /locus_tag="PLG24_01345"
     CDS             12756..>14810
                     /locus_tag="PLG24_01345"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ23555.1"
                     /translation="MTPKFLFLLILFFIIASVGTESMHAQHRKVTFHGTITDDATGET
                     LIGAALTIFPIKVSTVSNEYGYYSITFSESDSVTLVATYVGMEPLLLRLPVDKDLTVD
                     IRMKSGSVALDEVVISTDPLQEKMQGTQMGIEQISAKTSKVLPALFGEVDIIKTLQLK
                     VGVSSGSEGSAGLYVRGGAGDQNLILLDEATVYNANHLFGFFSTFNSDAIKDIRMYKG
                     GFPARYGGRLSSVIDVRMKDGNNRKLSGSGGIGLIASRLTLEGPIGSEHTSFIVSGRR
                     TYVDLITNLINKSKEDDPNWNKIPAYSFYDLNAKVNTRLGKNDRLYLSGYFGKDHFSL
                     KDSILDLGFSWGNATVTARWNHAFGNKLFANTSFIFSDYNYNISNQISGFNFSLGSKI
                     RNYAGKIDIDYNPNHRHAIRMGLSSTYYGFTVGRLKAGSDDGSISFSSGIERDGVEYG
                     VYVSDEFEVTNHLKANAGLRLSMWSSAGFFDAGLEPRLALAYEVDKRWSIKAGYARMK
                     QYIHLVSNSGLSLPTDVWYPSTSVVHPQLSDQVSAGFSYLIVKGLLLTNEYYYKKMSN
                     LVDFRDGANLFLNNEIEQEFEFGKGYSYGTEISLEKESGKVTGWIAYTLAFVKRGAFA
                     GIMDGRYFAPRYDRRHDVKVVVSYKFNRRWTTSATWVYGSGDLAWLPGGRAVFQDTKG
                     GTF"
BASE COUNT         3776 a         3186 c         3413 g         4435 t
ORIGIN      
        1 cggaaacttc caatctgcgc agaatttcat ccgcataagc gcaacttggg ttaattttgt
       61 ctattaagcc tcatttgcat tttgtctgaa tcaaacaacg ctattaatat caagaataaa
      121 ttgttgtatg caaaagaata ccaaaatcag ggtgagaagg aatgtatttt tttaatcgtt
      181 gaaaatggaa gacgtatctt tgtctcattc aatgtaatga tattcatccg ataaataatc
      241 cggatatgaa gaaacatgtg catatttatg atgcagcggg aaatcagttg tgttgcacgc
      301 tggaacaaaa gatcaatcag aagtcagatg tcacactggt tgctgctgag cctgcctgtt
      361 gcaatcacga tcatgcagat aatcatgtgg aagaacatga tcatattgcc tctgtagatg
      421 gcggatggtg gcacctgttc agacctgcga taatcagttt cgtgctattg atatccggta
      481 tcatcctgga tcattacgag ccctcttttt acgatcaatg ggttagaatt ggcctttatc
      541 ttcttgccta tatcccggta ggttggcctg tattgaagga ggcatgggag agtatagcca
      601 agggtgaggt gttttcggaa tttatgctca tgggtatcgc gacacttgga gcaatgggga
      661 tagagcaata ccctgaagct gttgcggtca tgctttttta ctcgattggg gagactttcc
      721 agacacttgc tgtccagcgt gctaaggcaa atatcaagat gatgctggat caaagacccg
      781 acgaagtcac catcgtcaat ggtgataaat ttgagacagt caaggcagct actgtgccac
      841 caggcaccat tgtgaggctc aatccgggcg agaaggctgc attggacggg atactgcttt
      901 cagcaagcgg gagttttaat actgctgcac tgacaggaga gagcgtacct gccacgaaat
      961 ttaagggcga agaaatcctg gcagggatga ttagcgtaaa cgaatcttgc aggatggagg
     1021 tgagcagacc ttatgaggac agtaaattgt ccggcattct ccggatggta caggatgcct
     1081 cttcacgcaa ggcccctacg gagttgttta taagaaaatt cgccaaggta tataccccaa
     1141 tagtggtgtt tctggcagtg gctatttgtc tgttgccttt tttcttcgtc tctgattata
     1201 tcttcagtca atggttgtac cgcgcattga tatttttagt tatttcctgt ccgtgtgcac
     1261 ttgtgatcag tattccactt ggttattttg gtggaatagg agcggccagc cgaaacggaa
     1321 tcttagtcaa gggaggtaat tatctggaca cactggccgg catacgtcat gtggtggtgg
     1381 acaagacggg taccatgacc cgtggtaatt ttgaggttcg tgaggttgaa atctcagaag
     1441 gttttaacag gatcgaagtc ctgaccgacc taaaggctct gcaggggttc agtacacatc
     1501 ccatagccgg agcgatccaa aggtatgttc aggaggaagt cgtaggcatc agggtagagg
     1561 atgttctgga aatcgcgggg aagggtatgt ccggcacagt caatggtaag catttactcg
     1621 cgggcaatct cgccttgatg caggtcaata aaattcctta tgatgtaagc cttgaagaga
     1681 tcgagtatac gctggtggca attgctgtgg acggcaaatt cgccggatat gtcacaatcg
     1741 ctgatgagat caaaccaaat gcagcagcag tcgtccgcga attggctgat atgggtgtag
     1801 aactgacgat gttgagtggc gacagtaaca aggttgtggc agccgtggcg cgggaaattg
     1861 gtatccgcaa agcctatggt ggtctcctgc ctgaagataa ggttgccaaa gccaatgaga
     1921 tcaaagaggc atcaccacca atagcctttg taggtgatgg catgaacgat gctcctgtcc
     1981 tggcactcag tgatgtaggc atcgccatgg gaggtttggg cagcgatatg gccatagaaa
     2041 gcgcagatgt cgtcattcag gatgatgaca tcaagaaaat cccgctctcc atccgtattg
     2101 gaaagaaaac caaaactatc gtatggcaga atatcctgtt tgcctttgga gtcaaggccg
     2161 cagtattgat tttaggggca tccggtgaag ccaccatgtg ggaagcagta tttgcggatg
     2221 tcggagttgc cctgattgcc gtggccaatg ctgtcaggat tcaacgaatg tagtgcagac
     2281 ttggaaaaat tgtccctttg tcgcaccaaa aatatataag tggcagacta tcaatattat
     2341 agacccaata tttcaccgca ctaaggttgg gaaaacgggg gtttgtgaat ccggtgaaaa
     2401 tctccaatgg acagaacctg ggttatttca gccaggagca attatttttt tatcgatcac
     2461 tgaaagaaat catcctcacc gggtttcctt ttcacggtgt ctatgggttg gtctataaca
     2521 ttgggattgc tgtactggct gcaatccgtc acaatatttt cggatccggc aggcacaatg
     2581 aatcttgcat tggggtcata atccgaaact ttttgtaact ccagctggcg gataaattca
     2641 gaaaagaatg gcctggccat cttacttccc tgtccgtatg taaggctgtt gaaatgaatc
     2701 cacctatcgt caccgcccac ccaagtaccg acaactaggc tcggggtaat gcccataaac
     2761 cagccatcga cataatcatt ggttgtaccg gttttgccgc ccacttcgct ctttattccc
     2821 tgaaatcctg ctgatccacg agtggcatat cgaagtaggt cgaccatgac gtagttgtag
     2881 tctggtgcca aagcctgttt ttcatcagaa gcattcttat agattacttt tccgtttttg
     2941 tcttcaattc tgctgataaa ggacggaacg acataggtgc cgttattggc aaatgatgca
     3001 taggctccgg tcatttccag gacggtgaga tcggcagaac caagacaaat tgacggctgg
     3061 gcgggtattt tagacttgtc aatacccata ttgttgacga gttccaaaac ctgatcggtt
     3121 gactggagtt gtttcataag atagacggat acggagttgc gggattctct gagcgcctca
     3181 taaagtgtca tacttttacc gctgaatttt ccttcggcat tgccgggtga ccatggcttg
     3241 atgaggccaa aactcgcttc tcccggttcg atggtatatt gctggtctac aacacgcatg
     3301 caggggctaa tgccacgaaa tgcaatagca gaggcataga cgaagggttt gaatgtggag
     3361 cctacctgtc ttttagtacg gatatggtca aacttaaaat atttgtgatt gatgccgccc
     3421 acccaaacct tgacctggcc tgttgtcggt tcgatggcta caatgcctgt ctgcaggaac
     3481 atccgatgat accggatgct gtcgtacggt gtcatcacag tatctttctg gccttcagca
     3541 ttgtaagcaa atactttcat tttatatttt tcattgaatg ctttgcgtac cgtggatcgg
     3601 aattgctgcc acttggctac ggtgatctta tattccggat cagccattat ttgctcatac
     3661 acgcgttttt gctcatctgt gatcctatat cttttggctg ttttatccag gccctctttt
     3721 tcggcagcag tccaaaggtc gaattcgata tctgaaaaac tgatctccgg gtattttgca
     3781 tttatttcat cgagttcttg ttcgaatatg ttctgtcgca tattgatata tcgatcggat
     3841 gagcgaacca ggttcttcat cgaagcttca cgaatggcca actgctttgc atcagcattg
     3901 tatttccagg ggtctttacc tttccattcc cgccaaaaat ttgtctggac ggtcttcatg
     3961 tgtttgagca tgacctgttc agcgatccgt tgcatatcgg catcgatgga agtatagatc
     4021 ttcaatccat ccctgtagat gtcataaagt gagccatccg acttttgcaa atccatcttt
     4081 cttatgattt gtgatacttc cttgcccact tccatccgga agtaggtagc atcaccatcg
     4141 atatgggtct ttggtttaaa atcaagttga atgggctctg cggaaagttt ttcatagtct
     4201 gactcagaca ggatgccatt gctgtacata cgtttgagga cggtattgcg cctgagggtt
     4261 gcttgttcac gccttcgcac cggattatac agggatggat ttttaagcat gcctaccagg
     4321 actgctgctt cggatatggt cagatccttg ttgttttttg aaaaatatgt ttcagcggca
     4381 gcttcgattc catatgcccc atagatgaag ttaaatttat tgagatacat agcgatgatc
     4441 tcttccttgg tataacggcg ttcgagcttg atggcagtga tccactcctt gagcttgata
     4501 ttgaccagag cgaacgactt tctaatccgg cccatattgc tgaaatcccg atcggaatac
     4561 aacaatttgg ccagctgttg agtgatggta ctaccgcctc ccgagctttt acgggccaac
     4621 aaaactgtct tggcgataac ccttgccaca gcttcggggt ctacaccact gtgtttttca
     4681 aaccggacat cttcagttgc aatcaacgcc ttgactatat tgggagagat ctgactgtag
     4741 gttattggta ccctgttctc aatgtacagt cgtcccagaa ccgagttgtc agaagcgatt
     4801 atgtcacttg caaaatcata cttcgggttt tccaattctt cgaaagtagg taggccggaa
     4861 agagatagga atatgatata agcaaatcca aaaaggacca atactcccag gcctatccac
     4921 atcttcctga cgatcgattt tattcgttcc atgttttctt ccataatcag aataaaaagt
     4981 gtgtttttat aaaacgaaag tacgaagaaa aaaatatata gcatggaaat gtaatatggc
     5041 catatggcaa ggcatccgtg agatagtgca tatgctcacc aatccttacc cgagcggtta
     5101 tattccggtt gttggcgctt catcaccttg ccgcgggtat ttttttcttc ttccttcatg
     5161 atgcgaagca aagactccgc ctcctgtggg ctgaggtgtt gaggtttgcc ctgattgttt
     5221 tgcttgtctt gtcgattgga ctgatttgca ttttgggctt gattacccgg ctcatttttt
     5281 tgttgattct gattattttg attctgatta ttttgattct ggttgtcttt ttgatcctga
     5341 tcttgtcttt ggtcctgatt cttatcctga ttcttttggt tattcttctt ctgttcgtct
     5401 tgcttgtttt tttctaccag ggcacgggtc aggttggcct tggcgtccag atctcccggg
     5461 tcgttgcgca aggcttgctt gtaggcatct atggcctttt cgtaatcctt ggtttggaaa
     5521 aatgagttgc cgagattgta agtgaccata tttttttgct gaggagtctt tgcatattgg
     5581 taggcttcca tatttttctc agcagcatcc ggatatttac ccagttgata cagggcattc
     5641 gcttcgttgt aaagactgtt gaaatccggc tttttcttgg cagcggcatc ataatgttct
     5701 gctgcttttt tataatcctt agcctgataa gcgatatttc cctccttcat aatggtgtga
     5761 gcactttggc cgtaagaggc gttatgaagc aggaagacca tcagggcggt tacagttgtt
     5821 acctttgtcc ggttgttact ttttgtctta atagaaatat tcatgctcat gatcatttca
     5881 atcaggaaaa ggaaaaatgc cggccagaga agccatggaa aaagactgat attattcgat
     5941 gtttcgtgga catcgtatgt tcccttgtca aggttatcta ccatattttt gacaccttcc
     6001 agggcggatc ggggattgtc aaggctatag tatcggccgt gagctgcagc ggcaattttt
     6061 attagttcct ctttattcag tttagaacga acgggctgac cttcttcgtc caacttatat
     6121 ttgaagccca gatcgtcctg ttcagggatg agagcgcctt tttcggtgcc tgctccgatg
     6181 gtggtaatta caatcccttt tttcgcggct ttctcggcag cctgaatggc attggagtcg
     6241 tggtcctccc catcggaaat gatgacaagg cctcggcttt tatttgtttc gtcgccaaat
     6301 gccttctgtg cggtctctat cgcatcacct attgctgtgc cctgataact tgccatttct
     6361 gtcgtgatcc cgttgatgaa atctatggcg gcattataat cgtcggtcaa tggcatatac
     6421 atataagcag ctcctgcaaa taccacaagg ccaatctgtt gccctttgag gatttggacc
     6481 agttctgccg cccaaagttt ggtcttggtg agtcggtcgg gtgctaaatc ctgtgcctgc
     6541 atacttcggc tgacatcaat ggccaggaaa atatctgatg ccctcttgat cgtcttttcc
     6601 ggtggaccgg gcaaacttgg tcctgccaat gccaggatgg ccaagatcaa tgcagtcaac
     6661 atcagggtta ttcgtgtccg ataattgtct tttggtggtt ctgctatttt ttgatatacc
     6721 tcctttccgg caaagtcagc tgcgattatt cgccgtcttt ttctccataa ggacatactg
     6781 atccccaata taggtaccac caagaggagg aggagatatt caggtcgttg cagtgtcaat
     6841 tgtcccattt agtatttaat aaataaaggt gtgcgcaccc aggtattttt caatatagtc
     6901 tctgtcaaaa tcagtccaat gcctatcaat aggaaccagg cgtattcatc atatcttttt
     6961 ttcaacacgg tcttttcgat cttggttttt tccaatagat tgattctgtt ataaatctgt
     7021 tgtaaggaat tttcatcctt agcccgaaaa tactggccat tagtcaggtt tgcgatttct
     7081 gtcatcaggg gctcatcgat ttcgacaggt acataaccgt atatgacctg tccgcttggt
     7141 gtcctgctga taggtgtttc tgcttcacct tcactgccga cacctatcac atatacccgt
     7201 aggtgataat cagcagccag cttagctgca gtaagtggat cgatataacc ggcagtattg
     7261 acaccatcag tcaacaggat gacgacctta ctttttgctt tagaatcttt cagggtgttc
     7321 actgccgaca ctagtcccat cccaatggcg gttccatcat ctaatgcacc ggcattgagg
     7381 ttgcgcagga tattgactac catacgatga tcataagtaa gagggcattg tgcaagtgct
     7441 tctcctgcaa agacggaaac gctgattctg tcgtttggcc gttctttgac aaattgctct
     7501 gcaagcgtct tggtgacctc aaggcgattg ggcttgaaat ctctagccag catactactc
     7561 gacaagtcta tcaccaaaga aatatctatt ccgctgtcgt tgatggtttc attcttttcg
     7621 agtctttgag gcccggccaa cgatatcagg ataaaggaaa gacctgcgaa tttgatccat
     7681 ggcaaatacc tgaaaagttt ggtgcgcaag gtgacgaagt ccttgactgc tgctgcagtt
     7741 ggcaaataaa cacctgaagt ccttcgttgt cgacgcaaaa tgatgaacat ccaccatagc
     7801 agtggaatga acaacagcac ccaggcaaaa cgaaaggcta tattaccaag ttcaagcata
     7861 tcggtccgga attttgcctt tgaatgattc tgcctcatcg attagagtct ccactttgcc
     7921 aataaaggag aaattgattt cctccctgct ttgcgctttg gcaaatttga ccaggtctgc
     7981 tatatttagt gccttgtccg ttgtatctat tacattgtaa ggtagccctg tttttttcaa
     8041 ttgggatact atctcatgtg atgtggattt taaggcaggt atatcgaatg cctcttgtaa
     8101 ataaatccgt aggatgaggg aaagttttgt ctgaaacatt ttgtggtctt cggccttcca
     8161 aagttcgttt tttctcaaca gcgccagttc ctttctggcc ttctgtgccg gactgatttg
     8221 catcacaggc gcttgtggcg tatatttact tcttcgtttc agatagctga ccaacaaggc
     8281 gcttagaacc cccagcaaga ttactgccaa ggatatgatg atgtacactc cgaacctgtc
     8341 ccaaaaggta atcacaacga caatatcctt attgggagca atgtcttcac ttttattgag
     8401 cataggcagt actttcaggc gccagggtct ggaaaagcct tttttttcgg tgttctgata
     8461 gtcataaaag aatggtacag ccggaaattc gtaatccccc ggttcgaagg ctgtgaacat
     8521 catttctttt gtaagtatct ttgacatttg cgtcttcctc tccacccatt ctttttcatc
     8581 acttagttca aagcctggaa ttgtagtgat ggagtcgaga accagcttct tgattgaaat
     8641 ggaggggtcg caggtaatgg taaatgtcag cttaattctg tcgcccagca agatgctgtt
     8701 ggtgtcggcc gttacctcca tttggatttg ggctgatacc gaaagtgccg aaaacgacat
     8761 caggcagaaa accaagacat gttttatcat ctgttcaatc atcgcgccct tgacttaaag
     8821 aagtacaaca atttacgcac aaagttctca tcctcaccca tggtgatttc aatctgatcg
     8881 accttactct cgccaaaaat tttttgtagt tttttctttc ttgcctcaaa ttgcttttga
     8941 taggctgttc taaaggccgg atccgatgta tcgagaacaa attcttcatt ggtctccgga
     9001 tccacggcaa tcagcatacc gacatccgga agttgatgct ctgccttgtc ttcgatatgg
     9061 atggcgatga ggtcatgctt gcgtcctgct atgcgcatct gggcgccaaa aagatcatcc
     9121 tgaaagtctg acagcataaa gcagatgctg tttttcttta ttacagtatt caggaagcgg
     9181 agtgcctttc tgatgtcggt attcttgttt tcaggtcgaa aatttaccat ttcccggatg
     9241 atgcgcagaa tttggttgcg tcctttggag gggggaatat acaattcgat tttatcggaa
     9301 aacaaaatca ggccgactct gtcgttgttt tgtaccgctg aaaaagctaa tacagcactc
     9361 atctcggtaa tcagttcgct tttaaggcgt gtaccggtcc cgaaaaagga cgacttgcta
     9421 acatcgacca taatgatcac attcaattca cgttcctcct catagacctt aacaaaaggt
     9481 acaccggtac gtgcagtaac gttccagtca ataaaccgga catcgtcact gtattggtat
     9541 tcgcgcactt ccgcaaatga catgccgcgt cctttgaaaa agctgagggt atcacccgag
     9601 aaaagaagcc gcgacatatc cctcgacttc agctctactt tcctgacttt acgcaacaac
     9661 tcattgactt ccatcagaac tgttgaaatt tgatcatcca atattcaggt ttccaccatt
     9721 tccatctaac gcctttgatt tttttgttgg tctgaaacaa ttgcctgtcg aaaataggat
     9781 catattccgt actgagcact attccgcgaa aggccaatac cggggcgccg tatgatgcat
     9841 ctttagctga aaaatcactt gcctcaggtt gcttggggat gattgaattc cattgtttgc
     9901 cgatgtcatt gggggaagat atcaggtctt caatattgac catgatattt tttttgtagt
     9961 tataattgac cggcatgata tgagaagtat tcctttcatt attcctgatg atgaagtgac
    10021 cacatagcca ctgaccagac acgtacgatg gttcaaacca agctaccatg gtgcctgccc
    10081 gggaataagg cctttcttct ccatgaatat ttttcaggtg tatatcgaac aaggagtcaa
    10141 actcatacct gatatcgttg accattatat tcaatttgtt ttccttgcct tttgtgccga
    10201 tgaccgggta atgtatctca aatgaaacca atctgtctga tttttctata agggctaatg
    10261 gaatctttat ccctgcagac attgcggaag ttttgccggg caaagtactt tgaataagga
    10321 tttcgtcatt gagtatacag gtcatgtttg ctccatcttg cggcagggag tagcttttca
    10381 ggttgtattt tctttctgtg ttatcagggc gtttccctga catgtgataa gtttttttta
    10441 agtcggtatt atggaggatt cccttacagg aatagtcatt gggaaaggaa agaacgatgg
    10501 tttcttccgg attggcaaca ccggtaaaaa ctacaattgc agggttatct atgggctcgg
    10561 gggtctttgc ttccacccag gatcccactg tcaatccttc cattttgccg gaatagactc
    10621 cggatgggga agaaagatct gctgtaaata cggtgccggc aaatattcct cgaatcatac
    10681 ctaatacctt ttgattcttg ctatattctg caagaaatat ggaatcaccc ttgaaaaagc
    10741 cctccacatc agtgtttttc ttggcagaga tcatctcata gtatccccgc acctcgtcac
    10801 ctttcctaca cagaaacatg cggaaggtgt gggttccatc cacccatccg ctgataacgt
    10861 attgccgatc gcttgcttcc ccttcccaaa gatctttaca gctggtcaat tgagcatagc
    10921 ccggcaagca aatgaaagca caaaacaaaa taacaaggaa ccgcatcact actatttaat
    10981 gtatgaaagt gcatcaaggc acattgacga tattaaggat ggatgtgatg atggattcac
    11041 cggtgaggcc ctctgcttca gcctcgtaag taatgcctat cctgtgtctt aagacatcca
    11101 tggctatcgc ccgcacatca tccggaatca cataggcccg acgtctgatg aaggccagag
    11161 ccttggatgc caatgccaga ttaatactcg cccggggaga agcgccatat tggatcatgg
    11221 gcgccagatc gggcagtccg gcagacttta catcccttgt agcgaatacg atgtcgagta
    11281 tatatctttc caaagacggg tcgagataga tggatttgat tatagctctg gcatctaaaa
    11341 tttcctgtgt tgtagccact ggttgttgga gttgtccgat tgaatcatcc atgtgtcgac
    11401 ggatgatatg ttgctcttcc tccttggtgg gatagtcgat tgtaaccttt agcatgaatc
    11461 ggtctagctg ggcttcgggc aatggataag tcccttcttg ttcgatggga ttctgggtag
    11521 ccataaccag aaaaggttct tcgagggcaa aggtctcctc gccgatagtg acctgcctct
    11581 cctgcatcgc ctccaatagt gcactttgca cctttgccgg tgcacggttg atttcatcag
    11641 ccagaataaa attggcaaat atcgggcctt tctttacgga gaatttgttg agagtgggat
    11701 tgtaaatcat ggtaccgaca atgtctgccg gcagtaggtc tggagtgaat tgaatccttg
    11761 aaaatttgcc gttaaccaag gtggcgaggg tcttaatggc gagggttttg gccagtccag
    11821 gcaagccctc caacaggatg tgacctttgg tgatcaggcc gattaaaagc ctttcgatca
    11881 tgtgttcctg accgacgatg atattggcag tggcgtcaga caggtgggat acaaattcac
    11941 tacttttgta cacccgctca ttcagcgctt ctatctcaga atattgcgtc ataaatatcg
    12001 ttttaatttc aataaaagtt tcgaatccat gcttttattg gcaacctctg ttgtcaggtt
    12061 ccaagcatgt caaccgtata aactatttaa gaacaaaaat agtatttcaa aggtcggaaa
    12121 attcccgcaa ttggccgaat ttgacaattt gttaaccgcg tttttattgt aaaactcctg
    12181 taagagtata atttggtgaa ttgatcaaaa ggtagaatag cattgatccc aggtaaataa
    12241 acaggaattt agaaaacacc ctcccaggac gccgggaatt accggcatcg catggtaggc
    12301 tgtaaaataa taaggtttat tgtgagtgat tggaaaaata ttggacgatt tgctctaaag
    12361 tatgttttaa acaggaaatc gggtaatgaa tatcagcaga aacaaaacga tcaacatgat
    12421 agcccagatt ctgacatcct taaacatttc atgtaacacc actttaactt cttttgcaac
    12481 aggtttcata ttctctcttt ttcatcaaaa accacaacat aaatcttaca aaaacgatac
    12541 cataacaata atctataata tttacttcgg aagtcgcttg gtaattatct tattataaat
    12601 gtttcgatgt ttgccgaagg ccatattaaa ataatttaaa ttcgtttgtc ttatgtattt
    12661 ttgtccttcg gattcgttat tttgaaaaac caaggctttt aacttttcat ccggcaaatg
    12721 agcatctcca tcctaagtat cttttaatcg ataagatgac cccaaaattc ttgtttttac
    12781 tcattctgtt ttttattatt gcttcagtgg gcaccgaatc aatgcatgca cagcatagaa
    12841 aggttacctt ccatggtacc attaccgatg atgcgacagg agagacgcta atcggtgccg
    12901 ctttaacaat cttccccatt aaagtatcga cggtgtccaa tgaatatggt tactattcga
    12961 ttactttttc tgaaagtgat tcagtgacac tggttgccac ctatgtaggg atggagcctt
    13021 tgttgctaag gcttcctgtc gataaggact taacagtcga tatacgcatg aaatcaggtt
    13081 ctgtcgcact tgatgaagtc gtcatctcga ctgatcccct acaggagaag atgcagggca
    13141 cacagatggg tattgagcag atatcagcta agaccagcaa ggtattgccc gcactatttg
    13201 gagaggtgga catcatcaag accctccaac tgaaagtcgg tgtctcatcc ggttcagagg
    13261 ggagtgcagg gctgtatgtc agaggaggtg ccggggacca gaatctgatc ttattggatg
    13321 aggccactgt atacaatgcc aaccatcttt tcggtttttt ctccaccttc aattctgatg
    13381 ccatcaaaga tatccggatg tacaagggtg gttttccggc gcggtatggt ggcaggctat
    13441 catctgtgat tgatgttcgc atgaaagatg gtaacaacag gaaattaagt ggttccggcg
    13501 gcatcggcct gatcgcatcg agattgaccc ttgagggccc aatcggcagt gagcatacaa
    13561 gcttcatagt gtcaggcagg cgtacttatg tggatctgat cacgaatctc atcaataaaa
    13621 gtaaggagga tgaccccaat tggaataaga ttccggcata tagcttttac gatcttaatg
    13681 ccaaggtcaa tacaaggctt ggtaagaatg accggctcta tctaagtgga tatttcggca
    13741 aggatcattt ttccctgaag gatagtattc ttgatcttgg gttcagctgg ggcaatgcta
    13801 ctgtaacggc tcggtggaac cacgcatttg gaaataaact atttgccaat acttccttta
    13861 tattttccga ttacaattat aatatatcca atcagatcag tggtttcaat ttttctttgg
    13921 gcagtaagat ccgcaattat gccggaaaaa ttgacataga ttacaatccc aaccatcggc
    13981 atgctatcag gatggggctt tcttctactt attacggatt taccgttggc aggcttaagg
    14041 ctggttctga tgacggctct atttcattca gcagtggaat cgaacgagat ggcgtcgaat
    14101 acggtgtgta tgtgagcgat gagtttgagg ttaccaatca cttgaaggcc aatgcaggat
    14161 tgcgattatc catgtggtca tccgccggtt tttttgatgc agggcttgag ccgcgccttg
    14221 cattggccta tgaggtagac aaaagatggt ccatcaaggc cgggtatgcc cgaatgaagc
    14281 aatacatcca ccttgtatca aattccggct tgtcgttacc gaccgatgta tggtaccctt
    14341 ctacctcggt tgttcatcct caactttctg atcaggtgtc ggcgggcttt agctatctga
    14401 ttgtcaaagg tcttctgctc accaatgaat attattataa aaagatgagc aatctggtag
    14461 attttaggga tggtgcaaac ttgtttctca acaatgaaat agaacaggag tttgaatttg
    14521 gcaaagggta ttcctatggc actgaaattt cccttgaaaa ggagtcggga aaggtgaccg
    14581 gctggatagc ttatacactg gcctttgtca agagaggtgc atttgcagga atcatggatg
    14641 ggagatattt cgcaccgaga tatgacaggc gtcatgatgt caaggtcgtg gtctcatata
    14701 agtttaaccg acgatggact acatcagcga cctgggtcta tgggtcaggt gacctggcgt
    14761 ggttgccggg aggaagagct gtgtttcagg atactaaagg cggcaccttc
//
LOCUS       DAOQQN010000019        14556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1343_length_14556_cov_7.466864, whole genome shotgun sequence.
ACCESSION   DAOQQN010000019 DAOQQN010000000
VERSION     DAOQQN010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14556
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1343_length_14556_cov_7.466864"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PLG24_01350"
     CDS             complement(<1..745)
                     /locus_tag="PLG24_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ23556.1"
                     /translation="MVKTAVRLDGIGEYYFSGKLREIDELNKAGKNIVNLGIGSPDLP
                     PHPEVIRTLLEESAKANTHAYQSYKGSPVLRKAISDWYLHWYGVNLNPDREVLPLIGS
                     KEGIMHICMTYLEKGSVALVPNPGYPTYSSAVKLSGAEPMFYDLKEENDFEPDFNQLE
                     SLITSASKGPLKGARMMFVNYPHMPTGKMASEQLFRRLVELGNRHGILIIHDNPYSFI
                     LNDRPQSLLSVEGAMDCVLELNSLSKSHNM"
     gene            complement(825..1733)
                     /locus_tag="PLG24_01355"
     CDS             complement(825..1733)
                     /locus_tag="PLG24_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HMZ23557.1"
                     /translation="MTEKKNPAAVQTSGDKSPISGTGRAASQLKVFIQGIEGSFHQIA
                     ARKFFGEEVSVICCNTFREVIAGASITDTDTYAVMAIENSIAGSILPNYNLLHQSRMV
                     ITGEIYLQIRHNLLAYPGNNIEDIREVHSHTMALQQCYDFLDHHPWKLVETEDTALSA
                     RNIQHDKSRHTAAIASTLAAELYGLEILASDIHTLKNNYTRFLVLQHEEMDRPSIQAD
                     KASVTFHTDHSKGSLARVLDRIAVGGINLSKLQSFPIPGTDFKYAFHADMEFDSLHQF
                     ERVIESLRSITESLRIYGEYRKGGFY"
     gene            complement(2149..2496)
                     /locus_tag="PLG24_01360"
     CDS             complement(2149..2496)
                     /locus_tag="PLG24_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HMZ23558.1"
                     /translation="MTIKEYQTRVDKWIKNHGVRYFDEMTNSLLLVEEVGEWARLIAR
                     TYGEQSFKSSESQTDTTTMLADEWADIFFVLTCLANQTGIDVEAAIHRNFEKKTNRDA
                     QRHKGNKKLGVEK"
     gene            complement(2493..2948)
                     /gene="dtd"
                     /locus_tag="PLG24_01365"
     CDS             complement(2493..2948)
                     /gene="dtd"
                     /locus_tag="PLG24_01365"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HMZ23559.1"
                     /translation="MRAVIQRVSSASVVINGTEHDAIGPGLLILLGITEEDTADDIDY
                     LVQKICMMRIFSDAEGKMNLSVRDKDGRIMVISQFTLFAQTRKGNRPSFIQAARPETA
                     IPLYEEFLLRMDMAMEQRTARGLFGADMKVSLINDGPVTIVIDSKNKDL"
     gene            3481..4572
                     /locus_tag="PLG24_01370"
     CDS             3481..4572
                     /locus_tag="PLG24_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HMZ23560.1"
                     /translation="MEAELIIKDRQNIEQHRHARVVRNWLWIGVIMISIQVMIGGITR
                     LTGSGLSITKWEIAIGTIPPLNEHQWVEAFDLYKDTPQYHKINKGMSMSEFKFIYFWE
                     YFHRLWARSLGFVFIIPFCWFLYKKMIHRSLMRDLIIIVLLGGIVGIFGWIMVASGLV
                     NRPWVNAYKLTLHLNLAFLVYAYLFWTTLKVTFPERSAAIIGKRIGIVKVINILLIVQ
                     LILGGVVSGMKAGLWYPTWPLMNGVYVPHDLLDFSNWNGNNFVEYDSYSFAASFFQFF
                     HRNVAYIIFILGFMIFFKWTGLLKDKRLFFNAMMIFIITLCFQVFIGIATVMSCKGSI
                     PVFLGVVHQLGAIGIISALLYMNFRFVKT"
     gene            4703..5470
                     /locus_tag="PLG24_01375"
     CDS             4703..5470
                     /locus_tag="PLG24_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ23561.1"
                     /translation="MDYPVITSGKFKYKQSREDGNVLLLLHGLFGGLSNFNELFDYFK
                     DSHRVVIPVLPIYELPLLKVSVSGLVDYIDEFVDYMGFGKVHVLGNSLGGHIAILYAL
                     KRSDKISSVILTGSSGLFESAMGSSYPRRGDYNYIKKKTEATFYDPAVATKELVDEVY
                     GIVNDRGKGIRIIATAKSAVRHNLENQLHNITAPTCLIWGNNDTITPPFVAEKFNELI
                     KNSELHFLDKCGHSPMMEIPEKFNVILADFLKRVEGA"
     gene            complement(5531..6847)
                     /gene="thrC"
                     /locus_tag="PLG24_01380"
     CDS             complement(5531..6847)
                     /gene="thrC"
                     /locus_tag="PLG24_01380"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HMZ23562.1"
                     /translation="MKYISTRGHNTRYSFDEIIRQGIPDDGGLFVPETIPTLPEEYYE
                     NITQKTFHEIALDIAGLFIDEDIVPRDVLKKIISDAFNFPLPLKNISSSLGIFELFHG
                     PTGAFKDFGARFMAQILSYLQSKKSEKVTIVTATSGDTGAAVASAFHNVNHVEVFILY
                     PKGRVSNFQEKQITGLGNNIHALEIKGTFDDCQELVKNCFNDHHFKNELNLTSSNSVN
                     FSRLIPQIIYYFEAYRQARVLGHENISFIVPSGNFGNLTGGVYAKKMGMPVKKLIAAT
                     NANDVFPNYVESGIYEPGNVSKHTISNAMDVANPSNMERIFFIYRSTWNNIVHDINSN
                     SIDDEITRLTISHFYKKNKYLADPHTAVGISNYNLENVYHGNTYFVVLSTAHPYKFKE
                     VIHEILPDVEIEKNNFIREYERTEIKKIPFNKDLDAIKEYIRKTVE"
     gene            complement(6863..7795)
                     /locus_tag="PLG24_01385"
     CDS             complement(6863..7795)
                     /locus_tag="PLG24_01385"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HMZ23563.1"
                     /translation="MSSGIKVFAPASVANLACGYDVLGLALEKPGDEIILRKSETKGL
                     KITSITGADRPLPKDIDKNTAGVAAISLLENIGMQDIGIEMEIHKKMPSGSGLGSSAA
                     SAAAAVWGINNLLHLGMTKRELLPFAVLGEQSADGAFHADNVAPSLMGGIIFIRDNVT
                     LDLFKLPYIQGLYLAMVHPALTILTKDARSIIAPDVTLQQHIQQSGNLAAFILATYTS
                     DINLLYRCLNDVIIEPQRASLIPHFYEVKDAAKQKGAIGCSISGAGPSIFALCDNSLI
                     AEEAAKAMADVFIKNKIEATYFTSKINHEGAIVL"
     gene            complement(7795..10260)
                     /gene="thrA"
                     /locus_tag="PLG24_01390"
     CDS             complement(7795..10260)
                     /gene="thrA"
                     /locus_tag="PLG24_01390"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HMZ23564.1"
                     /translation="MKVLKFGGSSLATGDRIKNVANIIRQSTTKKKNPIVVVSAFGGI
                     TDTLISCGNKASSGDEGYLTIQDEIINRTLSIAKELFPKKEGYNTFINKLNPIIDEFK
                     GLLKGIFLLKEMSDRSLALLLSFGERISALTLASYLTNSGQTSSFVDARGLIKTDHHH
                     LKAQVDFMATNHLITEFFKKHKETTIITGFIASTFDGTTTVLGRGGSDYSASIFGAAI
                     RADVIEIWTDVNGVLSADPRKVFDSFTIPQLTYDEASEISHFGAKVIYPPTVIPARKN
                     NIPLLIKNSFQPLHPGTIINNDKSKENTSIKGVTSISKVSMITLAGGGLVGVPGSAAK
                     LFQALSDQAINIILISQGSSENSISYVIDASESDKAVKAQEKAFEKEIAAGLIEPAKV
                     QNDLSIISIVGENMYSTPGIAGKMFRALGKNGINVYAIAQGSSELNISSVVHRDNEVK
                     SLKVLHEMFFLSKTIRVNLFMTGTGLIATTLLKQIEAQREHLKKVNNIEICLIGLSNS
                     RKMTFNKDGINLQSWQQELDNKGEKTDAKAFVQMCIDANLPNSIFVDCTASGEIPAYY
                     SQLLNHSISISTPNKISVSGEFGMYTELKEAARKNNVFFLHETTVGAGLPILNTLKNL
                     KESGDNILSIEGVFSGSVSYIFNNFSASNSFSKVVSQAAEKGYTEPDPRIDLNGIDVR
                     RKLVILSREAGFVINEKDFKIKTFLPKEVQNARAMEEFWEQIDKADAYFEKLLHKAKK
                     NKSKLRFIGTITSKGASLALKEVGKKSPFYNLDGSDNMIVFNTERYKERPLVIQGPGA
                     GAEVTASGIFAEILTIVTMLKDY"
     gene            complement(10690..10764)
                     /locus_tag="PLG24_01395"
     tRNA            complement(10690..10764)
                     /locus_tag="PLG24_01395"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10727..10729),aa:Val,seq:tac)
     gene            complement(10844..12265)
                     /locus_tag="PLG24_01400"
     CDS             complement(10844..12265)
                     /locus_tag="PLG24_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23565.1"
                     /translation="MKLKMIVFISWTIAFSLVTSFGGAQSLVLSGEIPMPNGHTFDLL
                     GYFNGKITLLEEDGRNVTLHQFDDDLQKINAPSIINNSKRSYLFGYVPQDSLLTLYYK
                     YFEDGEYIFSAVNYDPQLRLASSTALQKYIKNQTKAYPVGVSQDKNCHLITLDEAPSH
                     NSRFIMYQANSNIVLWELADPKILNDLYDDDKRGRIITNDGSAYMLYVDGRHRYRNNY
                     TLVALNKTGHSRLSIPLSPVPLTDIRLDYDNNQKQIIISGIYMDDGLTGQEGLLIIRL
                     DKNMKYEGEIKKIPFTRQTITDFYGQDQRPRNVLYDLTLNGCQERQDGGILIFCEQKK
                     EVSRLSNVGRSAFMPVSTSTDFYIEDIILTSLGPNNQLEWQKILQKKQYSYDDNAIFS
                     SYFIHANPSRLHLLYNDEIKNENTISEYIVNPFGNVERRVMFNTAGSNIQLQLRNSLQ
                     VSANSTIMPSSRKGKLRLLKITY"
     gene            12438..13559
                     /locus_tag="PLG24_01405"
     CDS             12438..13559
                     /locus_tag="PLG24_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23566.1"
                     /translation="MSDKLNLANSSKILSSSRFEKQYLMSALSTRIFIVGLGETKSLN
                     AAIRYFKGLDKKYNGFTSPFYFDKVLKHNGKFFWNPFLPTFFSEAFERYIINEFENLK
                     EDQGLLLPGVSYIDFTSCGSQSRLNTTIDLFVTKKFKLYDLTHKLYIKWNQPRSMNYM
                     LARFLNVLDLQTEPWLVIDDNAYEYDYESLSAKGIKGVIINLGNEYSEQFATKNIGYN
                     NNPIATLIRNIRNAGLIAGINLGIRYAMEDNLQLVLKNSKDLGTHFMIWQYDCDDLDA
                     EQDQRKINQAAIERAFLKAGSSKEYEQFPLIFYPEFFEKINKNKSSIRKYAAYSFENG
                     NMIQHSGPYTSLFREILTIRKAKPYNTQYSGIYAPATKR"
     gene            complement(13705..>14556)
                     /locus_tag="PLG24_01410"
     CDS             complement(13705..>14556)
                     /locus_tag="PLG24_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ23567.1"
                     /translation="AAELTAMMTDQKPFYASLLPSELAALINAPDPLTLERDVVKETK
                     EEEQDLNLGKFLPWLLLILGLCLVWYFMRNDFRKNMAGPVMSEHTTLDTAIQTNSDTI
                     ASRLSTIDDGMVNGNVDDMGNWVAELGGEYTLQLPNGKTLKVYENSIERQLVDFILTG
                     QKDESVLKQKWFTFDRLYFRQGKSELTEDSKLQIDNIAEILRAFTAVKIKMGGYTDSD
                     GNEALNLKLSEERSNIARKELLKRGIASARVEAEGYGESHPLCPANDTPACKARNRRI
                     DIRITGM"
BASE COUNT         4103 a         2827 c         2890 g         4736 t
ORIGIN      
        1 ccatattgtg acttttactg agtgagttca actcaagcac acagtccatg gcaccctcca
       61 cactcaggag actctgtggc ctgtcattga ggatgaaact atacggattg tcatgaatga
      121 taaggattcc gtgcctgttg cctaattcga ccagtctcct gaacaattgc tctgacgcca
      181 tcttacccgt cggcatgtgg ggataattga caaacatcat ccgggccccc ttcaatggcc
      241 ccttagatgc agaagtaata aggctttcca gttgattgaa atccggctca aaatcatttt
      301 cctccttcaa atcataaaac atcggctcag caccggataa ctttactgca ctcgaataag
      361 tcggatagcc cggattggga accaatgcca cacttccttt ttccaaatag gtcatacata
      421 tatgcatgat gccctcctta ctgcctatca atgggagaac ttcccgatcg ggattcaggt
      481 ttacaccata ccaatggagg taccagtcgc tgatcgcctt cctaagcacc ggagagccct
      541 tgtaggattg ataggcatgg gtgttggcct tggccgattc ctctaacaac gtcctgatca
      601 cttccgggtg tggcggcaag tccggactcc ctatacccag attgacaatg tttttgccgg
      661 ccttattcag ttcatctatc tccctgagct tgccggagaa atagtattcg ccgataccgt
      721 ctaatctaac agctgtcttt accatattct tatcatctgt atattttgtt ttacaatgga
      781 tatggatgga ttcattattg tcctaccata ccaaattctt cttatcaata aaagccgcct
      841 tttctatatt caccgtatat acgcagcgat tctgtaatcg atcggaggct ttctatcacc
      901 ctctcaaatt ggtgcagcga atcaaattcc atatcggcat ggaatgcgta tttgaagtca
      961 gtccccggaa tagggaagct ctggagcttg ctgaggttga tgccgccaac tgctattctg
     1021 tcgagtactc tggcaagact gcccttggaa tggtctgtat ggaaggtcac cgaagcttta
     1081 tctgcctgta tggaaggccg atccatttct tcgtgctgca acaccaggaa tcgcgtataa
     1141 ttgtttttaa gggtgtgaat atcggaggca agaatctcta gtccgtataa ttctgcggct
     1201 aaggtacttg cgatggccgc tgtgtgacgg cttttgtcgt gttgaatatt gcgggcactc
     1261 aaggccgtgt cttctgtttc gaccaatttc cacggatgat ggtccagaaa atcataacac
     1321 tgttgcaatg ccatagtatg cgagtgtact tcccggatat cctctatgtt attacccgga
     1381 taagctaata ggttgtgcct tatctgcaga taaatctccc cggttatgac catcctgctt
     1441 tggtgcagga ggttataatt gggaaggatg cttccggcga tggagttttc gatggccatc
     1501 acagcatagg tatcggtatc cgttatagat gctccggcaa tcacttcccg aaatgtattg
     1561 caacagatga cgctgacctc ttctccgaaa aactttcgcg cggctatctg atgaaagctg
     1621 ccttcaatgc cctggataaa caccttcaac tgtgatgccg ctcttccggt gccgcttatt
     1681 gggctcttgt ccccggatgt ttgtacagcg gctggatttt ttttctctgt catagttatc
     1741 acttctcata agtgcaaaaa aaaaatcccc cggcttttgg ccggaggatt ctctttatcg
     1801 ttgtaaaata attttattat tctatttcaa ccatagcatc tccggcctcc ttgcgtaaaa
     1861 gaagcaataa aaataaaagc cgaaatatga ttgtattgaa atcatgggga caaatgtaaa
     1921 cggtccggaa aatatttcca atattttttc aaaaaaagtg aaaaaaattt gtggattttc
     1981 attgaaatcg gtttgtgttt agagggatag gggattaatg aaaaataatt aataaaaaaa
     2041 ttatgatgta tactacactg cctggttccc aggtgttttc aacaccgatt caacccaata
     2101 atgtgtaaat attggccaac ccaaaaagtt taacgtatct tcctttggtt atttttctac
     2161 tcccaacttc ttatttcctt tatgtcgctg tgcgtcccga ttggttttct tttcaaagtt
     2221 gcggtgaatg gcagcttcta catcgatgcc ggtttgattt gcaaggcagg tgaggacaaa
     2281 gaagatgtca gcccactcat cggcaagcat ggtagtggtg tctgtttggg actccgaaga
     2341 tttaaacgat tgttcgccat aggtacgtgc gatgaggcgg gcccattccc cgacctcttc
     2401 cacaagcaga aggctgttag tcatctcatc aaaatagcgg acgccgtggt ttttaatcca
     2461 tttgtccacc ctggtttggt attccttgat tgtcatagat ctttattttt tgaatcaata
     2521 acgatggtta cgggaccatc attgattagg ctcaccttca tgtctgcacc aaaaaggcca
     2581 cgggcagtac gttgttccat agccatatcc atcctgagta aaaattcctc gtacagtggg
     2641 atggctgtct ccggtcttgc tgcctgtata aaggaaggcc gattgccttt gcgtgtctga
     2701 gcaaataagg taaactggct gatcaccata attctgccat ctttgtccct gacacttagg
     2761 ttcatctttc cttctgcatc ggaaaatatc cgcatcatac aaatcttctg taccagataa
     2821 tcgatgtcat ccgccgtgtc ttcctccgtg atgcccaaca atatcaataa tccgggaccg
     2881 atggcatcat gctccgttcc attaatgaca acagaggcac tactgactct ttgtataact
     2941 gctctcaagg ttaaaatgac ggtttattaa cattaaagat aaagttactt tatttgacgc
     3001 tccaaattaa ttttatacaa atcaacaaaa tagaccatat catacgacaa attgtgagta
     3061 actttttttg ttttgtggat aaataatatt ttaaaactta tattcattat ttttaaatat
     3121 gttagagtta tttcccaaat tcctcctctg aaaattccaa tgattacaca ttttatcacc
     3181 cagaattttc gtacatgaat aaaataaata tgaattgtgt atatgtttaa atatattata
     3241 tttatctgta tatcaatata ttataatgtt agttttctgt ggattattac ataatattgt
     3301 tagtaatgat atttcaaaat tatttcattt ttattatcca catattcaca gccttcaaca
     3361 acaactattt ttttctttat ataattttat tattttattt attaatatga gtgtgaggaa
     3421 aaatgtaggg tcgattaagt atattatcct aattttggtt ttcttaattg cgaataggaa
     3481 atggaagcag aacttattat taaggaccgg cagaatatcg aacaacacag acatgcccga
     3541 gttgtgcgga attggctttg gataggtgtc atcatgatca gcatccaggt gatgattggt
     3601 ggaattacga ggttgaccgg ttcaggcctt tccataacca agtgggagat tgccattgga
     3661 actattccac cgctgaatga acatcagtgg gtagaagctt ttgatcttta taaagacaca
     3721 ccacaatatc acaagatcaa taagggtatg tcgatgtcgg aattcaaatt catctatttt
     3781 tgggaatact ttcaccggct ttgggcacgc agtcttggat ttgttttcat cattcctttt
     3841 tgttggtttt tatataagaa gatgattcat agatccttga tgcgtgactt aatcattata
     3901 gttctgttgg gtggaatagt gggtatattt ggttggatca tggttgccag tggcttagtc
     3961 aaccgcccgt gggtcaatgc ctataagctg acgttgcact taaatctggc ctttctggtc
     4021 tatgcctatc tattctggac aaccctaaag gttacttttc cggaaaggtc tgcggcaatc
     4081 atcgggaagc ggataggaat cgtcaaggtt attaatattt tgcttattgt gcagctaata
     4141 ttgggtgggg ttgtatcagg tatgaaagca ggactctggt atcctacctg gccattgatg
     4201 aatggagtgt atgtgccgca tgacctgctg gatttcagta attggaatgg aaataatttt
     4261 gtggagtatg atagttattc ttttgctgca tcattctttc agtttttcca caggaatgtc
     4321 gcatatatta ttttcattct tggttttatg atatttttca aatggaccgg gttactgaaa
     4381 gataaaaggc tattcttcaa tgctatgatg atatttatta tcaccttgtg ttttcaggtt
     4441 tttataggta ttgcaactgt gatgagttgt aagggcagta taccggtttt tttgggtgta
     4501 gtacatcagt tgggcgccat tggtatcatc agtgccttat tgtatatgaa tttcaggttt
     4561 gtcaaaacct gatgattggt ttatgatcga tgcttttgtt ctatttagaa aattagaaag
     4621 catatgattg aaagttggct ttatattagt gtttgaataa aaaaaaataa ttaatctgaa
     4681 tttttttttc aatctttgtt aaatggatta tccggtaata acatcaggga agtttaaata
     4741 caaacaatcc agggaagatg gaaatgtatt gttgttgctg cacggtttgt ttggaggcct
     4801 cagtaatttc aatgaattgt ttgattattt taaggattca cacagggttg taattcctgt
     4861 acttccaatc tacgaattgc ctttattgaa ggtttctgta tcaggtctgg ttgattatat
     4921 tgacgagttt gtcgattata tgggtttcgg taaagtacat gtactgggaa attcattagg
     4981 gggacatata gctattctgt atgcactaaa gcgatccgat aagatatctt cagttattct
     5041 taccgggagc agcggattat ttgaaagtgc catgggtagt tcttatccac ggcgtggtga
     5101 ttacaattat attaaaaaga aaacagaagc taccttttat gaccctgctg ttgccactaa
     5161 ggagctggtg gacgaagtgt atggtatagt taatgacaga ggaaagggta ttaggataat
     5221 tgctactgcc aagtcagcgg tgcgtcataa ccttgaaaat cagctacaca atattactgc
     5281 acccacttgt ttgatttggg gaaataatga caccattaca ccaccttttg ttgctgaaaa
     5341 attcaatgaa ttaatcaaga attccgaact tcattttttg gataaatgtg ggcatagccc
     5401 gatgatggag attcctgaaa aattcaatgt tatacttgct gactttttga aaagagtgga
     5461 aggagcttag agttaagctt tattgttgga tattgggata tgataaaaag acaatgatat
     5521 agttgaggga tcattcaact gtttttctta tatattcctt gatagcatcc agatctttat
     5581 taaaggggat ttttttaatt tcagttcttt catattcacg aataaaatta tttttttcta
     5641 tttcaacatc cggtaatatt tcatggatta cttccttgaa tttgtatgga tgtgcagtag
     5701 ataataccac aaaataagta ttgccgtgat atacattttc caggttgtaa tttgaaattc
     5761 ctacggctgt atgcggatca gccaaatatt tgtttttctt gtaaaaatga gatatggtta
     5821 gcctggtgat ttcatcatca atggagttgc tatttatatc atgtacgata ttgttccacg
     5881 tggaacgata gataaaaaag attctttcca tattggatgg attggcaaca tccatagcat
     5941 ttgatatagt atgtttactc acattacctg gttcatatat accactttct acataatttg
     6001 ggaatacatc attggcattg gtagctgcga taagtttttt aacaggcata cccattttct
     6061 tggcatatac tcctcctgtt aaattgccaa aattgccact tggtacgata aacgaaatat
     6121 tttcatgacc aagtaccctt gcctgacggt atgcttcaaa ataataaata atctggggaa
     6181 tcaatcttga aaaattaacg ctgttggaag atgtcagatt caattcattt ttgaaatgat
     6241 gatcattaaa gcagtttttg accagttcct gacaatcatc aaaggttccc tttatttcca
     6301 gggcatggat attattaccg agaccggtta tttgtttctc ctgaaaatta gatacccttc
     6361 cttttggata tagaataaat acctcgacat ggttgacatt atggaaggct gaggcgacag
     6421 cggcaccagt gtcccctgaa gttgcagtga cgatggttac tttttctgat tttttagatt
     6481 gtaaataact aagaatttgt gccataaaac gcgctccaaa atccttaaat gctccggttg
     6541 gtccatggaa tagttcaaaa attcccaggc tggaagatat attttttaaa ggtaaaggaa
     6601 aattgaatgc atcgctgatg atttttttta gtacatcgcg cggtacgatg tcttcatcaa
     6661 tgaataaacc ggcaatatcc aatgcaatct cgtgaaatgt tttttgggtt atattttcat
     6721 aatattcttc gggtaatgta ggaatagttt ccggaacgaa aagtcctcca tcatcaggga
     6781 taccctgtct tataatttca tcaaaggaat atctggtatt atgtcctctt gtgcttatat
     6841 atttcatggt tactataaat tatcagagca caattgctcc ttcgtgattg attttagaag
     6901 taaaataggt ggcttcaatt ttatttttaa taaatacatc agccattgcc tttgctgctt
     6961 cttcagctat cagactattg tcgcacagtg caaatatcga tgggcctgct ccactgatgg
     7021 aacaccctat tgcacccttc tgttttgctg catcctttac ttcatagaaa tgaggaatga
     7081 gagatgccct ttgtggttct atgataacat cattcaaaca tcgatacagt aaattaatat
     7141 cggatgtata ggtagcgagt ataaatgctg ccagattccc cgattgttga atatgttgct
     7201 gcagggtcac atctggagca attatactcc tggcatcctt tgtaagaata gttaacgcag
     7261 gatgaaccat tgcgagatat aacccttgta tatatggcag tttgaataaa tctaaggtaa
     7321 cattatctct gataaatatt ataccgccca tcaatgatgg tgcaacatta tcagcgtgaa
     7381 aggcaccgtc agcactttgt tctcccaata cagcaaatgg caatagttca cgttttgtca
     7441 tacctaagtg taacagatta tttattcccc acactgctgc tgctgcactg gcggcacttg
     7501 aaccaaggcc actgcctgac ggcattttct tgtgtatttc catttctatt cctatatctt
     7561 gcatgccaat attctcaagt agcgatatag ctgcaactcc tgccgtattt ttgtcgatat
     7621 cttttggtaa agggcggtca gctcccgtta tggaagttat tttcagtcct tttgtttctg
     7681 attttcttaa aatgatttca tcccctggtt tttccagagc aagtccaagt acatcataac
     7741 cgcaggcaag attggcgaca ctggccggtg caaatacttt tattcccgag ctcatcaata
     7801 atcttttaac atagtaacaa tggtgagaat ttcagcaaag attccggatg cagttacctc
     7861 tgctcctgcg cccggcccct ggataaccaa gggtctttcc ttatatcttt ccgtattaaa
     7921 tacaatcata ttatcacttc catctaaatt atagaatgga ctcttttttc cgacttcttt
     7981 taatgcaaga ctggctcctt ttgatgttat ggtgcctatg aatcgaagtt tggacttgtt
     8041 tttctttgct ttatgcaata atttttcgaa ataagcatcc gctttgtcta tttgctccca
     8101 aaattcttcc attgcgcggg cgttctgtac ttctttaggc aggaaggttt tgatcttaaa
     8161 atccttttca tttattacaa atcccgcttc acgactcaga ataacaagtt ttctccgtac
     8221 atctattcca tttagatcga tgcggggatc tggttcggta tatccttttt cagcagcctg
     8281 acttactact ttggaaaatg agttacttgc cgaaaaatta ttaaaaatat atgagactga
     8341 tccgctaaat actccttcaa tagataatat attatccccg ctttctttaa gatttttcaa
     8401 agtattgaga attggaagac ctgctcctac ggttgtttca tgcaggaaaa agacattatt
     8461 ttttcgcgct gcttctttca gttcagtata cataccaaac tctcctgaaa ccgatatctt
     8521 attgggcgta cttattgaaa tgctgtgatt gagcaattga ctgtaatatg ccggtatctc
     8581 tcctgacgca gtgcaatcaa caaagatact attgggtagg ttggcatcaa tgcacatttg
     8641 aacaaatgct ttggcatccg ttttttcacc tttgttgtct agttcttgtt gccatgactg
     8701 caggttgatt ccatctttgt tgaaggtcat tttacgtgaa tttgataatc caataagaca
     8761 gatctctata ttgttgactt tcttgagatg ttcacgttgc gcttctattt gctttaaaag
     8821 ggtagtagca atcaaaccgg ttcctgtcat aaagagatta acgcggattg tttttgaaag
     8881 aaaaaacatt tcgtgcagga ctttgagact tttgacttca ttgtcccgat gaacgacgga
     8941 agaaatattt aattcgcttg aaccttgtgc aatggcatat acattgatac cgtttttacc
     9001 taaagcacga aacattttac cggctattcc gggggtgctg tacatatttt cgccgacaat
     9061 ggaaatgata ctcagatcat tttgcacttt ggccggttcg ataagccccg ccgctatttc
     9121 tttttcgaat gctttttcct gtgctttgac agccttatcg ctttctgaag catcaatgac
     9181 ataagagatt gaattttctg aagatccttg tgagattagg atgatattta ttgcctgatc
     9241 ggataaggcc tgaaataact tggcagcact gccgggtaca cccacaagac ctccacccgc
     9301 caaggtaatc atggagactt ttgaaatgga ggttactcct ttgatgcttg tgttttcctt
     9361 cgatttatcg ttattgatga tggtacccgg atggagtggt tggaaagaat tcttgatcaa
     9421 cagtggaatg ttgttttttc ttgccggaat gacagtaggt ggatatatta cctttgcgcc
     9481 gaaatgactg atttctgagg cttcatcata ggtaagttgg ggaattgtaa aggaatcaaa
     9541 taccttgcgc ggatcggcac ttaatacccc atttacatct gtccatatct caataacatc
     9601 agcacgtatg gcggcaccaa atatcgatgc tgagtagtca gaacctcctc gtcccaggac
     9661 tgttgtagtt ccgtcaaaag ttgaagcgat gaaaccggtg atgatggtag tctctttatg
     9721 ttttttgaaa aattctgtga tcaggtgatt ggtcgccatg aagtcaacct gtgctttgag
     9781 atgatgatgg tcagttttga ttaatcctct ggcatcaaca aacgatgagg tttggcctga
     9841 attggtcagg taggaagcaa gtgtgagtgc agaaatacgt tctccgaaag atagtagcaa
     9901 agccagagaa cggtcactca tttctttcaa aagaaatatg cctttgagta agcctttgaa
     9961 ctcgtcgatg atcgggttaa gcttatttat aaaagtatta tatccttctt tctttggaaa
    10021 taattccttt gcaatagaca gtgtcctgtt aataatttcg tcctggatgg tcagatagcc
    10081 ctcatcaccg gaacttgctt tgtttccaca cgaaataagg gtgtctgtga tgccaccaaa
    10141 agccgatact acgactatcg ggtttttctt tttagtggtg gattgccgga tgatgttggc
    10201 gacatttttt attctatcgc ctgttgcaag ggatgatcca ccgaatttca atactttcat
    10261 ggtgtattat tattaagaag gcaaaaatga taatatttca tcatatattg agaaaaaggc
    10321 tactatagaa ttgaataaag caacacattt aataaagata tatataaaaa attgtttcac
    10381 gtggaacaat cgggtggtgg cagggtgaaa cttttgcaac agctcaaaga ttagaataat
    10441 tactctaaaa aaggtagcac aacaattgat tccgggcttt atattttccg accaacatga
    10501 attttatttt ctgaatttat tttcctaatt gtataagata agattggatg tgattagcct
    10561 gtatatttca gcataaagta atcaaccttt cacagcatat agagattaat atatagtcag
    10621 ataataggtg acaaggatag cagggtaaga acaaaaaaag ggtccccaac aggaaccctt
    10681 ttcattttgt gggagcaaag ggattcgaac ccctgaccct ctgcttgtaa ggcagatgct
    10741 ctgaaccagc tgagctatgc tcccttcttt ttaattacct ttctttaaaa gcgttgcaaa
    10801 gatatgggtt tttccaattt tgcaaagttt ttcacacatt tttttaatag gttattttaa
    10861 ggagccttaa ttttcccttg cgactgctcg gcatgatggt actattagca ctgacctgca
    10921 gggaattcct caattgtaac tggatattgc tacccgccgt attgaacatg acccggcgct
    10981 ctacgttgcc aaacggattg actatgtatt cactgatggt attttcattc ttgatttcat
    11041 cgttgtacaa tagatgtagt cgggaaggat tggcgtgtat gaaataggat gagaagatag
    11101 cattatcatc ataggaatac tgtttctttt gaagtatttt ttgccattcc aactggttat
    11161 tgggtcctag actggtcaga ataatgtcct caatatagaa atctgtactg gtactcaccg
    11221 gcataaaggc acttcttcct acattactca acctgcttac ttcttttttt tgttcacaaa
    11281 atatcagtat ccctccatcc tgcctttcct gacaaccatt taaagttaga tcataaagca
    11341 catttctcgg acgttggtcc tgaccataaa aatccgttat cgtttgacgg gtaaacggaa
    11401 tcttttttat ttcaccctca tacttcatat ttttatccaa acggatgatc agtaaaccct
    11461 cttgtccggt cagtccatca tccatatata ttcccgatat aatgatttgt ttttgattat
    11521 tgtcataatc caacctgata tcagtcagtg gaacaggaga taatggaatg gataatctgg
    11581 aatgtcctgt tttatttaaa gcgacaagag tatagttatt gcggtatctg tgccttccat
    11641 ccacgtacag catgtatgcc gatccatcat tggtaatgat tcggcctcgc ttatcgtcat
    11701 catagagatc attcagaatt ttcggatcgg ccaactccca aagtacgata ttggagtttg
    11761 cctgatacat gataaatcga ctgttatggg acggtgcttc gtccaaggtg atcagatggc
    11821 agtttttgtc ttgtgataca cccaccggat atgccttggt ttggtttttt atatattttt
    11881 gcagagcagt gctgctcgct aaccgcaact gaggatcata gttaaccgca gaaaaaatat
    11941 actcaccgtc ttcaaaatat ttgtaataaa gagtcagcaa actgtcttga ggtacatagc
    12001 caaacaggta actgcgtttg ctattattga tgatcgaagg agcattgatc ttctgaagat
    12061 catcatcaaa ctggtgcaaa gtaacatttc ttccatcctc ttccaacagg gttatctttc
    12121 cgttaaaata gcccaacaag tcaaaggtgt gaccattggg cataggtatt tcgccggaaa
    12181 gcaccagtga ctgggcacca ccaaatgagg taaccaaact aaaggctatt gtccatgaaa
    12241 taaagactat cattttgagt ttcatacccc aattttaaca aaattttaag acggactttc
    12301 gacggaatgg gattaaaaag tagttacttt attaacccaa atgaatagaa aatcagtcaa
    12361 tattttctca cttaatcgca aattaatcaa attgcttgaa ataatagaat tgatttttaa
    12421 atatggaaaa gaaaactatg tccgataaat taaacctggc gaacagttca aagattctca
    12481 gttcgagccg gtttgaaaag caatatctaa tgtcggcgct ttctaccaga atattcattg
    12541 ttggtcttgg ggaaacaaaa agtctcaatg cggccatcag gtatttcaaa ggacttgata
    12601 agaaatacaa tggatttacc agcccatttt actttgacaa agtgctgaaa cataatggta
    12661 aatttttttg gaatccattt ttgccgactt ttttcagtga agcattcgaa cgatacatca
    12721 tcaatgaatt tgaaaacctg aaggaagacc agggactgtt gttacccggg gtttcttata
    12781 tcgattttac aagttgtggc agtcagagtc gacttaatac tacgatagat ctttttgtaa
    12841 caaaaaaatt caaattgtat gacctgaccc ataaattgta tatcaagtgg aatcaacccc
    12901 gatccatgaa ctatatgctt gcccgattcc tgaatgtatt ggatcttcag acggagcctt
    12961 ggcttgtcat tgatgataat gcctatgaat acgattatga atcattatcg gccaaaggaa
    13021 taaaaggtgt gatcatcaac cttggaaatg aatattcaga acaatttgca acaaaaaata
    13081 ttgggtataa taataatccc atagctaccc tgatcagaaa catcagaaat gccggattga
    13141 ttgcagggat caacttaggt atccgttatg ccatggaaga taaccttcaa ttggtgctta
    13201 aaaattccaa ggacctgggc actcatttta tgatttggca gtatgactgt gatgatttgg
    13261 atgcagagca ggatcagagg aaaataaacc aggccgctat agagagagcc tttttgaagg
    13321 caggaagtag taaggagtat gaacagtttc ctttgatatt ttaccctgaa ttttttgaaa
    13381 aaataaataa gaataaatca tcaatccgga agtatgctgc atactcattt gaaaatggca
    13441 atatgattca acattccggt ccatatacct ctcttttccg tgaaattttg accatcagaa
    13501 aagccaagcc atacaatacc caatattccg ggatatatgc acctgccaca aaaagatgat
    13561 ggacagatcg agattggcag actcctagct tggtgtcgga agtgcgagct taaaaaaagg
    13621 aatgacaaaa gaatgtatat cgccaggcga tgagcaatca cattttgcct catttatatc
    13681 attgaagaca ggaatatact tctgttacat tccggtgatt ctaatatcta tccgtctgtt
    13741 ccttgccttg catgccggcg tatcattggc cggacatagt ggatgtgact ctccataacc
    13801 ttccgcttcg accctggcag atgctatgcc gcgctttaac aattcctttc gggcaatgtt
    13861 ggacctttct tcagaaagct taagattcaa tgcctcattg ccatcggagt cggtatatcc
    13921 ccccattttt attttcactg cagtgaatgc tcgtagtatt tctgcgatat tgtctatttg
    13981 aagtttggag tcctctgtta gttcagattt cccttgccgg aaatacaaac gatcaaaggt
    14041 aaaccatttt tgcttcagga cggattcgtc cttttgtccc gtcaggatga aatcaactaa
    14101 ctggcgctct atggaatttt catacacttt cagtgtcttc ccattaggga gttgaagggt
    14161 atattctcca cccaactcag ccacccagtt gcccatatca tccacattgc cattgaccat
    14221 accgtcatca atagtggaga gtcgggatgc aatagtgtcg gagttggttt gaatcgcagt
    14281 gtccaacgtt gtatgttccg acataacagg ccccgccata tttttcctaa agtcatttcg
    14341 cataaaatac caaacaaggc aaagaccaag aatcagcaaa agccaaggca ggaactttcc
    14401 aagattcaag tcttgttcct cctccttcgt ttcttttacc acatctctct caagcgtcaa
    14461 tggatccgga gcattgatca atgcagccaa ttctgaaggc aacaaggatg cataaaatgg
    14521 tttctggtct gtcatcatgg ctgtgagctc ggcagc
//
LOCUS       DAOQQN010000020        14452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1364_length_14452_cov_5.189762, whole genome shotgun sequence.
ACCESSION   DAOQQN010000020 DAOQQN010000000
VERSION     DAOQQN010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14452
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1364_length_14452_cov_5.189762"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1645)
                     /locus_tag="PLG24_01415"
     CDS             complement(<1..1645)
                     /locus_tag="PLG24_01415"
                     /inference="COORDINATES: protein motif:HMM:NF020677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HMZ23568.1"
                     /translation="MNNHIRFLLLFAVICLSSSVTAQFKFSDKEVTKVVYSTSYNDRT
                     DPNQNPIWVFADSGNALITNRDQMNNTAAFPFEMTLIHSNISSYAQYAFLKEGSCIAM
                     LDSQSINKQNWELLSDTKDILGYRCKKARTSINSNTIEIYYTDEMKIKGGPTLLGTGL
                     GFVLEMVRNGNYRISAIGTSKERFEWPAFLKNVTPEYLEKIDYNDRIWQSRFITIPVF
                     NHQQICFTDGPFDNSNEVMRFANGTIVVKKVRFPKIGQHDQIFLDMTQRSNGDAYDRT
                     GSVFLIPVDKRVSFLDALQKGIGELPIFDNGNGKKYQGVVLTPQYTPLLELMRFFTPF
                     GIGHFRDRVTLKGKTWLDSITYRQEVSDYAGELNDKEVYIGAFIGNYDKGGHIISMNF
                     TIHKEEDMDMPTRKVLPLFNTLNVMEMAGQDYGTMFDSEKGLKVEFELKETMKNCKLN
                     FITTGHGGWGRGDEFVRKKNTITLNDKIIFELIPWREDCGTYRLYNPVSGNFSNGLSS
                     SDYSRSNWCPGTVTNPYIIDLGDLPVGKHVLTVKIPQGKP"
     gene            complement(1723..3798)
                     /locus_tag="PLG24_01420"
     CDS             complement(1723..3798)
                     /locus_tag="PLG24_01420"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HMZ23569.1"
                     /translation="MRYKHFLLTLIAGTIAFSCSEPNNSKKNEHSQNTVSGYDPNFKV
                     SAQEFADLKVLRYQVPGFDQLPLQQKILAYYLSEAALSGRDIIYDQQSKNGLTIRKTL
                     EAIYGTYSGDKSTENWKNFEIYCGRFWFSNGNHHHYGNEKFFPEVSFEYFKEIAKASD
                     PNLFPMDSGEDLDTFLERIKPIIYDPNVEPKLVDLRPNIDNVKASSVNFYENVSLRDV
                     ELFYEKFDSKGNAPMWGLNSKLVKENGKVFEKVWKSGGMYGPAIDKIIYWLEKASGVA
                     ENDQQRKAINLLIDYYKTGDLHTFDNYCIAWVNDTASRTDAVNGFIEVYQDPLGRKAT
                     YESVVSIKDLEATKRIQAIAKQAQWFEDNSPLMPEHKKKNVKGIIAKAITVLMEAGAT
                     APAGPIGINLPNNEWIRKEHGSKSVSLSNIVHSYNVDGATSGMLDEFVVNDEVKARIK
                     EFGDITSDLHTDMHEVIGHASGQINPGVESTDKTLKNYASTLEEARADLVALYYMIDP
                     KLVEIGVMPTVDAGKAEYDSYMMNGLMTQLTRIQPGNNLEEAHMRNRQLVAKWAYENG
                     KASNVVEFVKKDGKTYVRINDYNKLRQLFGQLLREIQRIKSEGDYKAGHDLVENYGVK
                     VDPELHREVLERFKKLNIKPYKGFIQPRYVPVMKGDKIIDVKVEYPDNFFTQMMEYGK
                     KYSFLPVKN"
     gene            complement(3975..5177)
                     /locus_tag="PLG24_01425"
     CDS             complement(3975..5177)
                     /locus_tag="PLG24_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HMZ23570.1"
                     /translation="MDYFKEALFLHKQLKGKISVQTKFTIKNRDDLSLVYSPGVAAPC
                     LEIAEKPETVYDYTLKGNTVAIVSDGSAVLGLGNIGAAAAIPVMEGKAMLFKRFANIN
                     AFPICLDTQNTQEIIDTVKRIAPVFGGINLEDISAPRCLEIENALQDLGIPVFHDDQH
                     GTAVVTLAGLINASKLLDKPLSEMRVVINGAGAAGIAIARLLRCVEHTDNSVCQPVKE
                     IIICDTKGIIHRSRTDLTDVKKSLLAFSNSENMQGTVKDAIRNADVFIGVSSASLLDR
                     HDIATMNKDSIIFALANPIPEIMPDEAYAGGAAIVGTGRSDFPNQVNNVLGFPGIFRG
                     ALDVRAPRISPSMKMAAAYAIADCIDQPTKENIIPATLNDQVAYKVAEAVRKAAMKEW
                     NLKQIYGF"
     gene            complement(5189..5911)
                     /locus_tag="PLG24_01430"
     CDS             complement(5189..5911)
                     /locus_tag="PLG24_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HMZ23571.1"
                     /translation="MSKETSKGQPRNMRQWNIVKGENSWTLFKVISEFVDGFETLNRI
                     GPCVSIFGSARTKPGSKYYESAVQIARRLTEEGYGIITGGGPGIMEAGNKGAYENNGM
                     SVGLNIQLPFEQNHNPFIDPDKNLDHRFFFVRKVMFVKYAQAFVVMPGGFGTLDEMFE
                     VLTLMQTNKIKKVPVVLYGIEYWSGLKKWIKEVVGEMEHNINPIDLDLMPITDDIEEV
                     VRIINEYYEGDKQVRLRPNYEL"
     gene            complement(6140..7510)
                     /gene="radA"
                     /locus_tag="PLG24_01435"
     CDS             complement(6140..7510)
                     /gene="radA"
                     /locus_tag="PLG24_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HMZ23572.1"
                     /translation="MAKAKTVYICSSCGANSPKWIGKCNSCGEWNTYQEEIIQSSNTN
                     RNYSSRTGESGPKVSDKPKLLKEVKSNSHERIPTGDDELDRVLGGGIVPGSVILIGGH
                     PGIGKSTLILQSAMSMKTTVLYVSGEESEEQIKMRADRIGNISGQCYIFGETNIDTIL
                     RQADALQPSAIVLDSIQTMFLEHLESSAGSISQIRETAAELQRYAKERNIAVIIIGHI
                     TKDGDLAGPKLLEHIVDTVLYFEGDRHYAYRLLRSRKNRFGSTDEIGIYEMRESGLTP
                     VINPSDVLSGHGGQSYSGNALGIIGEGSRTFMLEVQALVTRAVYGTPQRSATGYDTRR
                     MNMLLAVLEKKCQLPLGFNDVFLNVAGGIKVDDPSIDLALAAAIISSLQNVAVPDKFC
                     FVGEVGLSGEVRPVQKIDQKLLEAERMGIDTIFISKYSKYNVPANFRLNIQPIVKVEE
                     LLDLFS"
     gene            7739..8551
                     /locus_tag="PLG24_01440"
     CDS             7739..8551
                     /locus_tag="PLG24_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HMZ23573.1"
                     /translation="MANVVSERFVLAIKKLIKDGNIKSMRRISLELDYLPQSLSEVTK
                     GKRDVPVEVLQRFITRFNVNPIFLFMGEGEIFLDQDQQYSFKTLTIVTDSDNEERILH
                     VPVPAQAGYAEELNAPEMIRELPTYTLPDITYNSGTYRSFDVRGDSMEPTIDENDILV
                     CSYLEPSLWETGIRDHHVYVIVTRGDVVVKRVINNLRKHKHLELYSDNEEYKMYRVYG
                     NDIREVWYVNTKISKFRHNQSTPAQAYTQGEFSALRDTIKAQAELIKTLTKH"
     gene            complement(8580..9224)
                     /locus_tag="PLG24_01445"
     CDS             complement(8580..9224)
                     /locus_tag="PLG24_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMZ23574.1"
                     /translation="MNERRKAKFEEVASKSQLSLTVILENVHDVLNIGAILRSCDSVG
                     IRNIFVINNSGLKQHKSLTLGKRSSMGTRKWVDVRYFHDLDTCIEEVRKDYDVIAGAA
                     ICEKTINYLDFDYTGKVAILMGNEKEGLSKAALDHCDVLIRIPQVGMAESLNVSTATA
                     VILYEAFRQRLVAGKYDHLPDISEGQANELLNRLHEKVFDKTKSTKKYKIKAEE"
     gene            complement(9217..10602)
                     /locus_tag="PLG24_01450"
     CDS             complement(9217..10602)
                     /locus_tag="PLG24_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMZ23575.1"
                     /translation="MLRSDKYISINPYTGNLIREFPYWDGKQIDQSIRSSKQAYHFWS
                     HTTIEERKSFLAALSEFLKLKADDWAVIITEEMGKPLSQSKAEILKSATLCDYYYQQL
                     EVLSQPRMIPLDNKRYGMVKPQAQGAVLGMMPWNFPIWQAIRFAVPVISGGNVVLLKH
                     AQNVGITANLIEEAITHAAGLEGVLINTFITHDDIARYLQDEHIVGASLTGSERAGVA
                     VGRIAGINIKKTVMELGGSDVFIVLEDADLELSAEIAVKARLNNNGQTCIAAKRFYIH
                     KNIYPKWKESFVEIIKSKKIGDPKDHSNELSGLARNDLAETLQAQVDKTVSMGARKLL
                     SGGVDKDNPSIYHPEIIEHIDSKSPLSCEETFGPVAALFPFEDEQEMLSRVNASRYGL
                     GASVWSTDTDHAIAIADQVNAGSVAVNQMMSSDPRLPFGGIKKSGFGREMSMEGFLEF
                     LNLKTIIVSNE"
     gene            complement(10586..11896)
                     /locus_tag="PLG24_01455"
     CDS             complement(10586..11896)
                     /locus_tag="PLG24_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ23576.1"
                     /translation="MRLRSFIVLAITLILVSCKSTTRVVYPEKKDKTSIPEIKNPERP
                     DSTHHSKIVKQNKKDSVGNEVIKPDANPVVIPTEKTKPNKHLKNNIKVAVALPMKGGY
                     DVSRFVEFVNGMSLAGSLSGGTSTDRLDIHFMDIGNDNSAEKLASIKAILDADIILGG
                     YQTSQVKALAEIALSKAIPYISLWNSSDDIVNHNPYFFQMKPSLEAYCSNMASYITKQ
                     LRPDLTIILLEGKNTKDASTIDYFSNIYQKERINFKVVYGLGSVDWQTLFMNYNRIVV
                     NIPNWDERQFVKKALKQLIEAKKNKNLTVTGMPQWASWDQADFTLFENCGLLVPIATS
                     VDLNAPEVIIFQKNYFEKYGTWPTSESFYGNDILYLIKTISSNLMKGDSFEPVNGYSA
                     KYFSSYEFRPVVTRPGGEVPQLSYYRNQSITIEKFEGGRFVTVR"
     gene            complement(11935..13479)
                     /gene="guaA"
                     /locus_tag="PLG24_01460"
     CDS             complement(11935..13479)
                     /gene="guaA"
                     /locus_tag="PLG24_01460"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HMZ23577.1"
                     /translation="MTHQKVIILDCGSQYTQLIARRVRELNIFCEIFPYNKVPQIETD
                     VKALIISGSPFSVRDNNAPEIDYKNILGKLPMLGVCYGAQYLAQKEGGRVERSEQREY
                     GKAILNIETPDDPIFSGIPASTQVWMSHGDSVLELPPGYTNIATTEHNKFAMFKKNPI
                     NDEAPVYAVQFHPEVTHSSDGLKFLGNFLLNIANIKADWTPDAFIEESIDQLKTKIGD
                     EKVLMALSGGVDSTVCATLLQKAIGDNLICFFIDNGLLRKNEFNNVLNAYKDLKLNVV
                     GIDASDYFYEQLSGKTDPEAKRKIIGKGFIDIFEREAGKLGDISWLGQGTIYPDVIES
                     ISVHGPSVTIKSHHNVGGLPEHLKLKLIEPLRMLFKDEVRRVGIALGLQPQFVHRHPF
                     PGPGLGVRILGDITAEKVRILQDADDIFISGLRKSGLYDQVWQAGVVLLPVRSVGVMG
                     DERTYENVVALRAVSSTDGMTAEWSRLPHDFLADISSEIINKVKGVNRVVYDVSTKPP
                     ATIEWE"
     gene            complement(13520..>14452)
                     /locus_tag="PLG24_01465"
     CDS             complement(13520..>14452)
                     /locus_tag="PLG24_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HMZ23578.1"
                     /translation="FEENQKGSLEAGLGITYLFGQGATGKGKMSMNKLKKLDSDGDGI
                     PDIQDECPHTAGVAAFNGCPDKDNDGIPDYQDKCPDEAGSRTTMGCPDKDGDGVPDKD
                     DKCPDIAGDAKYSGCPFIDSDGDGVPDGEDLCPDQKGLVRYQGCPDTDGDGVPDNLDQ
                     CKDKAGPAETNGCPDTDGDGIPDIEDKCPTVAGIPELNGCPAANQADLNILTNVSRNI
                     KWQDNAFALSNDARNQLDKIVSLLKKNKKYKLTVIGYSDSDTPKDQITSLSNARADEV
                     KGYLVSKGIEEQRIIAKGGNLPKISTRKILFEVK"
BASE COUNT         3977 a         3151 c         2745 g         4579 t
ORIGIN      
        1 ccggtttacc ctgaggtatt ttgactgtta atacatgctt tccgacaggt agatcaccca
       61 ggtcgatgat atagggattg gtaacagttc ccggacacca gttagatcga ctatagtcgg
      121 aggaggataa cccattggaa aaattacccg ataccggatt atagagtctg taggtgccgc
      181 agtcttcccg ccagggaatc aattcgaata taattttatc attgagcgta attgtatttt
      241 ttttacgtac gaattcatct cccctgcccc atccaccatg tccggtagta ataaaattta
      301 atttgcaatt cttcatggtc tccttgagtt caaactccac tttcagtcct ttttctgaat
      361 caaacatagt tccataatcc tgtccggcca tctccattac attcaatgta ttaaataatg
      421 gtagtacttt gcgtgtgggc atatccatat cttcttcctt atgaatggta aagttcattg
      481 aaatgatatg tccacccttg tcataatttc ctataaatgc gccgatgtac acctccttat
      541 cgtttaattc accggcataa tccgaaactt cctggcgata ggttattgaa tcaagccatg
      601 ttttaccttt cagtgttaca cggtctctga agtgacctat tccaaaaggg gtaaaaaatc
      661 tcatcagttc aagtaagggc gtatattggg gtgttagaac aaccccctga tattttttcc
      721 catttccatt atcgaatatg ggcagctccc cgataccttt ttgaagtgca tccaagaaac
      781 tgaccctttt atctacagga ataaggaata cggatccggt tctgtcatat gcatcaccat
      841 tggatcgctg agtcatatcc agaaatatct ggtcgtgttg tccgattttg gggaatctga
      901 ctttttttac cacgatggtt ccatttgcaa atcgcataac ttcgttactg ttatcaaaag
      961 ggccatctgt aaagcatatc tgttggtggt tgaatacagg tatcgtaata aaacggcttt
     1021 gccatatcct gtcattataa tcgattttct ccaggtattc cggtgttaca tttttcagaa
     1081 aagccggcca ttcgaatctt tcttttgaag ttccgatcgc agaaatcctg tagttgccgt
     1141 ttcgtaccat ttcgagaacg aatccaaggc ctgtgcctaa aagcgtaggg cctcccttaa
     1201 ttttcatttc atctgtgtaa tagatttcaa tggtgttgga attgatgctg gtccgggcct
     1261 ttttgcaccg gtagccgaga atatccttag tgtcagacaa taactcccag ttctgtttat
     1321 ttattgactg gctatccaac atggcaatac agctaccttc tttaaggaat gcatattgag
     1381 cgtaactact gatgttgcta tgtattaatg tcatttcaaa agggaatgca gccgtattgt
     1441 tcatctgatc tctgttggta atcagggcat ttccggaatc ggcaaacacc caaattggat
     1501 tttgattcgg atcagtcctg tcattataag aagtactata tacaaccttc gtcacttctt
     1561 tatcggaaaa tttgaattgt gctgtcactg aagaggacag gcaaatgaca gcaaataaaa
     1621 gtaagaaacg gatgtggtta ttcatcaagt tcaatattat cgtcaaacaa tgtccattat
     1681 ctttttcagg atatatgaaa aagcatgatt aattaatgaa aatcaattct ttacaggtaa
     1741 aaaggaatac tttttaccat attccatcat ttgagtgaag aaattatccg gatactctac
     1801 ttttacatca ataatcttat cacctttcat gacaggtaca tatctgggtt gaataaaacc
     1861 tttgtaaggc ttgatattca attttttaaa acgctccaac acttctctgt gaagttcagg
     1921 atccaccttg actccataat tttcgaccag gtcgtgaccg gctttgtaat caccctcaga
     1981 tttgattcgt tgtatttctc ttagtaactg tccgaataac tgtcgaagtt tgttgtaatc
     2041 gttgattctg acatatgttt tgccatcttt ttttacaaat tcaactacat tggaagcttt
     2101 cccattttca taggcccatt tagcaacgag ctggcggttt ctcatatggg cttcttccag
     2161 gttgttaccg ggttggattc ttgtcaattg ggtcatcaaa ccattcatca tgtatgaatc
     2221 gtactctgcc ttaccggcat caacagttgg cattacacct atttcgacca atttggggtc
     2281 tatcatataa tatagagcaa ccaaatcagc tctggcttcc tccagtgttg aagcatagtt
     2341 cttaagggtt ttgtcagtag attcaacacc tggattgatc tgtccggatg catgtccgat
     2401 aacctcgtgc atatcagtat gcaggtcact ggtgatatct ccaaactctt ttattctggc
     2461 tttcacttca tcattgacaa cgaattcgtc gagcataccg cttgtagcgc catcgacatt
     2521 ataagagtgg acaatgttgc tcagagatac agattttgat ccatgttcct ttctgatcca
     2581 ttcattattg ggaaggttga tgccgatagg gccggcagga gctgttgcgc cggcttccat
     2641 aagcaccgtg atggccttgg ctatgatacc tttgacattc ttctttttat gctcaggcat
     2701 caagggcgaa ttgtcttcaa accattgtgc ctgctttgct attgcctgga ttctttttgt
     2761 ggcttcaaga tccttgatac tgacaacact ctcataagtt gcctttcgtc caagaggatc
     2821 ctggtagact tcaatgaaac cgttgacggc gtccgttctg cttgctgtat cattaaccca
     2881 tgcaatgcaa tagttgtcga aagtgtggag gtctccggtt ttatagtaat caatcagtag
     2941 gttaatagct tttctctgtt gatcgttctc ggcgactccg gatgctttct ctaaccaata
     3001 gattatttta tcgatagcgg gaccatacat accgcctgac ttccatactt tttcaaaaac
     3061 cttgccattt tcctttacca gtttactatt caagccccac atgggtgcat tacccttgct
     3121 gtcaaacttt tcataaaata attcaacgtc cctcaaactg acattttcgt aaaaattaac
     3181 agaagaggcc tttacattat cgatattagg tcgcaaatcg accaatttgg gttctacatt
     3241 tggatcataa atgattggtt tgattctttc aaggaaggta tcaaggtctt caccactatc
     3301 cattgggaac aaattggggt cgctagcttt tgcaatttcc ttaaagtatt caaaggagac
     3361 ttcgggaaag aatttttcat tgccataatg atgatgattg ccattgctaa accaaaaacg
     3421 cccgcaatag atttcgaaat tcttccagtt ttcggtagac ttatctcctg aataagttcc
     3481 gtaaatagcc tcaagagtct tcctaatggt aagtccattc ttactctgtt ggtcatagat
     3541 gatgtctcgt cctgaaagtg cagcctctga taggtaataa gcaagtattt tttgttgtaa
     3601 tggcaactgg tcaaatccag gtacctgata tctaagaacc ttcagatcag caaattcctg
     3661 cgctgatact ttgaaattag gatcgtagcc cgaaacagta ttttgtgaat gttcgttctt
     3721 tttagaatta ttgggttctg agcaagaaaa agcgattgtt cctgcaatta aagtcaatag
     3781 aaaatgtttg tatctcacct ttaatgaatt tagaaaaagg ctaaaataca attttatatc
     3841 aatcacttaa aatacaatga aagaaaagta aggtaattac ttcctttgat gttggaaaac
     3901 aacacaggac tatgtgtctc cactttaaaa atgattcatc ccacgcttaa tctttccgaa
     3961 aaaccaaatc aagatcagaa accgtatatc tgcttgagat tccattcttt cattgctgcc
     4021 tttcttactg cttcggctac cttgtaggcc acttgatcat ttagcgttgc aggaataata
     4081 ttctcttttg taggctgatc aatacaatca gcaatggcat aggctgcggc cattttcatg
     4141 gagggagata tccttggggc acgtacatcc aaagcacccc tgaagattcc ggggaatccg
     4201 agtacattgt tcacctgatt ggggaaatca cttctgcctg ttcctacgat tgctgcccca
     4261 ccggcatatg cctcatccgg cataatctcc gggatcgggt tggccaaggc aaagatgatg
     4321 gaatccttgt tcattgtggc aatatcatgt ctgtcaagca aactggcact gctgacacct
     4381 ataaatacat cggcattcct tattgcatcc ttgacagtcc cctgcatatt ttcgctatta
     4441 gagaatgcca acagagattt ttttacatca gtcagatctg ttctgcttct atgaatgatg
     4501 cctttggtat cacaaatgat gatttccttg acaggctggc atactgaatt atcagtatgt
     4561 tccacacaac gcaggagtct ggctatggca atacctgcag caccggcacc atttataact
     4621 accctcatct cactcaatgg cttatcaagt agtttagaag cgttgatcaa tcctgcaagg
     4681 gtaacgacag ctgtaccatg ttggtcatca tgaaataccg gaatacctaa atcctggaga
     4741 gcgttttcaa tttcaaggca acggggcgca ctgatatctt caagattgat tccaccgaat
     4801 acaggtgcaa ttctttttac tgtatctatg atctcctggg tattctgagt atccagacaa
     4861 atcgggaatg cattgatatt ggcaaaacgc ttaaaaagca ttgccttgcc ttccataact
     4921 ggtattgccg ccgcagctcc gatattaccc aaacccaaaa cagccgaacc atcagaaact
     4981 atggctacag tattaccttt tagagtgtaa tcgtacactg tctcaggttt ttcggctatt
     5041 tcgaggcaag gagcagctac tccgggagaa tacaccaggg aaagatcgtc cctgtttttg
     5101 atggtaaatt tagtctgaac ggaaatcttt cctttcaact gtttgtgcag gaatagagct
     5161 tccttaaaat aatccattgt gtttattatt atagttcgta attggggcga agcctcactt
     5221 gtttatcccc ctcatagtat tcattgatta tacgcaccac ctcttcaata tcatctgtga
     5281 taggcatcag gtccagatcg attgggttga tgttatgttc catctcacca actacttcct
     5341 tgatccattt cttcagacct gaccagtatt caattccgta taaaacaacc ggtaccttct
     5401 tgattttgtt tgtctgcatc aatgttaaaa cttcaaacat ttcatccagg gtgccaaatc
     5461 ctcccggcat gacgacaaaa gcttgtgcgt atttgacaaa catcaccttc ctgacaaaga
     5521 aaaacctgtg atctaaattt ttatcaggat cgataaaggg gttgtgattt tgttcaaatg
     5581 gtaattgaat gtttaagcct acagacatac cattattctc ataggcgccc ttgttacctg
     5641 cttccatgat gccgggtccg ccccctgtaa taataccgta accttcttca gtaagcctgc
     5701 gcgcaatctg gacggcactt tcatagtatt tacttcccgg ttttgtacgg gcagaaccaa
     5761 aaatggacac acacgggcct atcctgttca aggtttcaaa accatcgaca aattcggaaa
     5821 ttactttgaa cagggtccat gaattctctc cttttacaat attccactgc cgcatatttc
     5881 gcggttgtcc cttacttgtt tctttactca tattaacata acatattcac gttcaataaa
     5941 atctattcat tcaaataaaa ttgaaagcct attgaattca tttcaaattt gacctttgat
     6001 tagacaaaga cagtcctttt agggcaaaaa accgataaaa atacaaaaat tcgaattatt
     6061 atatcaattt ttaaccgact tcaataaaag caattatgcc ataaatgagc atttgcctca
     6121 acagcataca aagcttctat tagctgaaaa ggtctagcaa ttcttcgact tttacaatag
     6181 gttgaatatt aagccgaaag ttggcaggaa cattatactt tgaatatttc gaaataaaaa
     6241 tggtatcaat acccatcctt tcagcttcca gcaatttctg atcaattttt tgaacaggtc
     6301 ttacttcacc actcagcccc acctcgccca cgaagcagaa tttatccggt acagcaacat
     6361 tttgcaggga agatatgatt gctgctgcca aagcaagatc aattgaagga tcgtccactt
     6421 ttattccgcc ggcaacattg agaaagacat cattaaagcc tagtggtaac tggcattttt
     6481 tttcaagtac tgccaacagc atattcatcc tcctggtgtc gtatccggtt gcggagcgct
     6541 ggggcgtacc gtaaactgcc cgagtaacaa gtgcctgaac ttcaagcatg aaagtgcgtg
     6601 acccctcacc tattattccc agggcatttc cactatacga ttggccccca tgacctgaca
     6661 acacatcgct tggattaatc acaggtgtaa gaccggattc tcgcatctca tagataccga
     6721 tttcatctgt agatccaaat cggtttttcc ttgaacgcag taatctgtag gcataatgtc
     6781 gatctccctc aaaatatagt acggtatcca caatgtgctc aagtaatttg ggtccggcta
     6841 ggtctccatc cttagttata tgccctatga tgatgacggc gatatttctt tccttggcat
     6901 atcgttgaag ttcagcagca gtttctctga tttgactaat gctaccggca gagctctcca
     6961 agtgttccaa aaacatggtt tgtatgctgt ctagaactat tgcagaaggt tgtagtgcat
     7021 ctgcttgtct taaaattgta tctatattgg tctctccaaa gatgtaacat tgtcctgaga
     7081 tatttccgat tctatctgct ctcatcttga tttgctcttc gctttcctcg ccggagacat
     7141 aaaggacggt agtcttcatg gacattgctg attgaaggat gagcgtagat ttgccgatgc
     7201 ccggatgacc acctatgagg ataacgctac cgggtactat tccgcctccc aagacacgat
     7261 ctaattcgtc atcgcccgta ggtatccttt cgtgtgaatt tgattttact tctttcagta
     7321 attttggttt atcggaaact tttggtccgg attctccggt acgtgatgaa taattccgat
     7381 tagtatttga tgactgtatg atttcttcct gataggtatt ccattctcca catgaattgc
     7441 attttcctat ccatttaggg gaattagcac cacagctgga acatatatat acagtttttg
     7501 ctttagccaa ggtaaattaa ataattttgc ctgcaaaggt atgattaaaa ggcgataaag
     7561 ccaagtatcc attgatcaac aaagccaata taaacataat cgggacaaga aagggaggat
     7621 aatatcattc actattttgc gtataatttt atgatgtgta aatattatga taatatatgt
     7681 gtgaaaaata tataatattt taataccttt gaacctgatt aatacgaatt aataaaatat
     7741 ggcaaatgtt gtgtcggagc gttttgtgct agcaattaag aaactgatta aagatggtaa
     7801 tatcaaaagc atgcggagga ttagccttga gctggattac cttccacaaa gtctcagcga
     7861 agtaaccaaa ggcaagaggg atgttcctgt tgaagtgttg caacgattta taacccggtt
     7921 caatgtcaat cctatatttc tctttatggg tgagggagaa atctttctgg atcaggatca
     7981 gcaatattca ttcaagactc tcacaattgt tacggattcg gacaatgagg aaagaatcct
     8041 ccatgttccg gtacccgcac aagcaggtta tgccgaagaa ttgaatgctc cggagatgat
     8101 ccgggaacta cctacctata cattgcctga tatcacctat aattcaggca cctaccgtag
     8161 ttttgatgtt aggggggatt cgatggagcc caccatcgat gagaacgata tcctggtctg
     8221 tagttatctg gaaccctctc tttgggaaac cggcatccgg gaccatcatg tctacgtaat
     8281 cgtcaccaga ggggatgttg tagtaaagcg agtaataaat aatcttagaa agcacaaaca
     8341 ccttgagttg tactcagaca atgaggaata caaaatgtac agggtctatg gaaatgatat
     8401 ccgggaggtc tggtatgtca acacaaagat ttcaaagttc agacacaatc agtctacacc
     8461 ggctcaagca tatactcagg gtgaattcag tgctttgagg gatactatta aagctcaggc
     8521 tgaattaatc aagactttaa cgaagcattg atgactgatt cttcggatgc atgatttcac
     8581 tattcttcag ccttgatttt gtattttttc gtactcttgg ttttgtcaaa aactttttca
     8641 tggagtctgt taagcaattc gttagcttga ccttctgaaa tatccggtag atggtcatac
     8701 ttacccgcta ccaatcgctg tctgaatgct tcatataata tgacagccgt tgctgtggat
     8761 acatttaaac tttcagccat ccctacctga gggattcgga tcaggacatc acaatggtct
     8821 agagcagctt tagacagacc ttccttctca tttcccatta aaattgcgac cttgcccgta
     8881 taatcaaaat caagatagtt tattgttttc tcgcagattg ccgcaccggc aatgacatcg
     8941 taatctttcc tgacttcttc aatgcaggta tctaaatcat ggaagtatct gacatcgacc
     9001 cattttctgg tacccatgga acttcttttt cctaaagtca gtgatttatg ctgttttaag
     9061 cctgaattgt tgattacaaa aatattacgg atacctactg aatcacagga tctaagaatg
     9121 gcgcctatat taagtacatc gtggacattt tccagaataa cggtgagtga aagctgagat
     9181 tttgatgcga cttcctcaaa ttttgccttt cgtctttcat tcattactga caatgattgt
     9241 tttcaggttg agaaattcga gaaaaccctc catactcatt tctcttccga atccggattt
     9301 tttaatacct ccaaaaggca atctggggtc actgctcatc atttggttca cagcaacaga
     9361 acccgcattc acctgatcag caatggctat ggcgtgatct gtatcggttg accatacact
     9421 tgctcccagg ccataccggc ttgcattaac ccgactaagc atttcctgct catcctcaaa
     9481 gggaaataaa gcggcaacag gaccaaaggt ttcttcacaa gatagagggc ttttgctgtc
     9541 gatatgctca attatttccg gatggtagat agatggattg tctttatcga caccgccgga
     9601 gagtaatttt cttgcaccca ttgaaacagt cttatcgacc tgggcctgca aggtctccgc
     9661 aagatcattt ctggccaggc cgctcaattc attggaatga tccttcgggt cgccaatctt
     9721 cttgctttta ataatctcga caaaagattc tttccacttt ggatagatat ttttgtggat
     9781 atagaatctc ttagcagcta tgcaggtttg accattgttg tttaagcgtg ctttgacagc
     9841 aatttcagct gaaagttcca gatcggcatc ctccaaaaca atgaaaacat cgctgccccc
     9901 cagttccatc accgtttttt taatgttgat accggcgatg cgtcctacag ctactcctgc
     9961 cctttcgctt cccgtcaggg atgcccccac gatatgttca tcctgtaagt accgggcaat
    10021 atcatcgtga gtaataaagg tattgataag tacaccttcc aggcctgctg catgggttat
    10081 cgcttcttca atcaaattag ctgtgattcc aacattctgt gcatgtttca gcaggacaac
    10141 atttccaccg cttatgaccg gtactgcaaa gcgtatagct tgccaaatcg ggaaattcca
    10201 cggcatcatg ccgagtactg ccccctgagc ctgtggtttg accataccat agcgtttatt
    10261 gtcaagcgga atcatgcgag gctgtgacag tacttccaac tgttggtaat aataatcaca
    10321 cagtgtggcg gatttaagga tttcagcttt gctctgggac aatggtttac ccatttcttc
    10381 ggtaatgatt actgcccaat catctgcttt caatttcaaa aattcagaca aagctgcaag
    10441 gaaggacttt cgctcttcta tggtagtatg gctccagaaa tggtatgcct gtttggagga
    10501 tcttatagat tgatctatct gcttgccgtc ccaatatgga aattctctta ttaggtttcc
    10561 tgtatagggg ttgattgaaa tatatttatc tgaccgtaac aaaacgccct ccttcaaatt
    10621 tttctatcgt tatggactga tttcggtaat aggacaattg tggtacttct cctccaggtc
    10681 tggttactac aggtctgaat tcataggatg aaaaatattt agcagaataa ccatttaccg
    10741 gttcaaagga atctcctttc atcaagtttg aggaaatagt ttttatcaaa taaagtatat
    10801 catttccata aaaggattct gaagtaggcc atgttccata tttttcgaaa taatttttct
    10861 gaaatataat gacttcagga gcattaagat caacactagt tgctatgggc actaacagtc
    10921 cgcaattttc aaatagagta aaatcagcct gatcccagct tgcccattga ggcataccgg
    10981 taacagtcag gtttttattt tttttagcct caattaattg cttcaatgct tttttgacaa
    11041 attgtctttc atcccaatta ggaatattaa caactattct gttataattc ataaaaagtg
    11101 tttgccaatc gacacttcca aggccataga ctaccttaaa atttatcctt tctttttgat
    11161 atatgttcga aaagtaatct atcgttgatg catccttagt attctttccc tcaagcaaaa
    11221 taatggtcaa atcaggcctc aattgctttg taatgtagct ggccatgttt gagcaatagg
    11281 cttccaatga aggtttcatt tgaaaaaaat acggattgtg attaactata tcatctgaac
    11341 tattccagag tgaaatgtag ggtattgctt tcgaaagggc tatttcagct aaagctttta
    11401 cctgactggt ttgatatcca cccaggatga tatctgcatc caggattgcc ttaatagatg
    11461 ccagtttctc tgcactgttg tcatttccaa tatccataaa atggatgtct agtctatccg
    11521 ttgatgttcc accggataaa gacccggcga ggctcatacc attgacaaac tcaacaaaac
    11581 gacttacatc gtaccctcct ttcatgggaa gggcgacagc aaccttgata ttatttttta
    11641 aatgtttatt cggtttggtt ttttcggtag gtattaccac aggattagca tccggcttaa
    11701 taacctcatt gccaacagaa tcttttttat tttgtttgac tattttggaa tggtgtgtac
    11761 tatcgggacg ctcaggattc ttgatttccg gtattgatgt tttgtccttt ttttcaggat
    11821 aaactaccct ggtcgttgat ttacaactga ctaaaattaa ggtaatagca agtacaataa
    11881 atgaccgtaa ccgcatatcg ttgtctgaat tttcgtggga ctatagcgaa acagttattc
    11941 ccactcaatt gttgcgggtg gctttgtact gacatcgtaa actactctgt taactccttt
    12001 tactttattg atgatctcag aagatatatc ggcgaggaag tcatgcggta atctactcca
    12061 ttcagcagtc atgccgtcgg tacttgaaac agcccgtaat gccaccacgt tttcgtaagt
    12121 acgttcatca cccataaccc cgacagatct gacaggcaat aatacaacgc ctgcctgcca
    12181 gacctgatca tagagtcctg atttgcgcaa gccgcttata aagatatcgt cagcgtcctg
    12241 aagtattcta accttttccg cagtaatatc tcctaatatt ctaactccga gaccaggtcc
    12301 cgggaaggga tgcctatgta caaattgtgg ttgtaagcct aatgcaatgc ccacacggcg
    12361 aacctcatcc ttaaaaagca tacgaagggg ctcaataagc ttcaatttca aatgctcagg
    12421 taatcctccc acattatgat gggatttaat agtgacactt ggcccatgca ctgagatgga
    12481 ttctattaca tccggataaa tggtaccttg accgagccaa cttatatctc caagtttacc
    12541 tgcttccctt tcaaagatat ctatgaagcc ttttccaata atcttgcgct tagcttcggg
    12601 atctgtcttt ccggaaagct gttcgtaaaa atagtcggat gcatcgatgc ctacaacatt
    12661 cagcttcaga tccttatagg catttaatac attattaaat tcatttttac gcaacaatcc
    12721 attgtcaata aagaagcaga tcaggttgtc gcctattgcc ttctgaagta aggtagcaca
    12781 aactgtacta tcaacaccac cggagagggc cataagtact ttttcatcac cgatttttgt
    12841 cttcaactga tcaattgatt cctcaatgaa ggcatccggc gtccaatcag cctttatatt
    12901 ggcgatattc aataaaaagt tacccagaaa ttttaagcca tcactgctgt gggtaacctc
    12961 gggatggaac tgtactgcat atacgggagc ctcgtcatta atcgggtttt tcttgaacat
    13021 tgcaaatttg ttatgttctg tggttgcaat gtttgtataa cccggaggaa gttccaatac
    13081 tgaatctcca tgggacatcc atacctgggt tgatgccgga attccggaga atatcgggtc
    13141 atcaggggtc tcaatattca agatggcttt cccatattct ctctgctcac tacgttcaac
    13201 ccggccacct tccttttgag ccaaatattg cgcaccataa caaacaccca gcattggtaa
    13261 cttacccaaa atgtttttgt aatcaatttc cggagcgtta ttatcacgaa cactaaaagg
    13321 acttcctgaa ataattaatg ctttgacatc ggtttctatt tgaggaactt tgttgtatgg
    13381 aaatatttca cagaaaatat tcaattccct tactctcctg gctattagtt gagtatattg
    13441 ggagccgcaa tcaagtatga taaccttttg gtgtgtcata gttcagatag gatttgagta
    13501 gcaaaggtat aacccctgat tatttaactt caaataaaat ttttcttgtt gatattttag
    13561 gtaagttacc acctttggca attatccttt gttcttcaat tcctttactt acaagatacc
    13621 ctttgacctc atctgccctt gcgttgctca gggaggtgat ttgatctttt ggtgtatctg
    13681 aatccgaata gccaatcaca gtcagcttgt atttcttgtt ctttttaagt aaggaaacaa
    13741 ttttatccag ttgattacga gcatcattgg acagggcaaa tgcattatcc tgccatttga
    13801 tatttctgga tacatttgtc aaaatattca aatcagcctg gttggcagcc ggacaaccat
    13861 ttaattccgg aattcctgcg acagttggac atttatcctc tatgtcaggg attccatctc
    13921 cgtcagtgtc cggacaaccg ttagtttctg ccggccctgc tttatcttta cattgatcta
    13981 aattatcagg tacaccatcg ccatccgtgt ccgggcaacc ctgataacgg acaagtcctt
    14041 tttggtccgg gcatagatct tcaccatccg ggacgccatc accgtctgag tcaataaatg
    14101 ggcaaccgct gtattttgca tcgccggcaa tatccggaca tttatcatct ttatcgggca
    14161 caccgtcacc atctttatcc gggcaaccca tggtggttct tgaaccggct tcatcagggc
    14221 atttatcctg ataatccggt atgccgtcat tgtctttgtc agggcatcca ttgaaagcag
    14281 caactccggc ggtatgcgga cattcatcct gaatatcggg aatgccatca ccgtcactgt
    14341 ccagtttttt cagtttattc atactcattt tgcccttacc ggtagcacct tgtccaaata
    14401 ggtaggtaat acctaagcca gcctccagtg aacccttctg attttcttca aa
//
LOCUS       DAOQQN010000021        14322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1380_length_14322_cov_6.220789, whole genome shotgun sequence.
ACCESSION   DAOQQN010000021 DAOQQN010000000
VERSION     DAOQQN010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14322
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1380_length_14322_cov_6.220789"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PLG24_01470"
     CDS             <1..389
                     /locus_tag="PLG24_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ23579.1"
                     /translation="IHLPPDIFNRNIRLLSGGQRQRISFLRALLANKPVLFGDEPTGN
                     LDPITAKTLMTMVREDISRSGSSAIIVSHDLNLAHLFADRIVTLKVDENRQCGIIGGI
                     FERKDNGWLDQLSHKNVEHAEITSLL"
     gene            386..1867
                     /locus_tag="PLG24_01475"
     CDS             386..1867
                     /locus_tag="PLG24_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ23580.1"
                     /translation="MKEIRSIFIKNEIKKLFQHGRSHFILLGIVFSLLIIALNQSIFG
                     LKALNEKYNNPFANYIQVATPKNTDLDRETLAKTLIQKFSAEPLKGKLGISRINELSY
                     EFMPFCNPAIDKVKTYKGRTYSKDDPLYQELVKASGDNIITTNKIEEDDITGQYGVII
                     TENMVLDLGYDPRSIKTVPLKLGGKEFVLVPVIVVVKYLPQFSDFMITEDFSYINNQS
                     IEQTNFVDLGSSNFYSIASFNDTSKVLEDARKVLKKDFKDVQCKLIDIDQLNKVSVCE
                     FQLNEYLSAEDKEDMTASLNTGNNKDKLYNFYTLHYVNTENAEKFAPDYLEFQFASLD
                     SIKAFQEVCMQNSLDPDMQIIKSRHLFAEVGSISRIVSGGFILFSILMLFIFTNSFIS
                     RHIEEIKPNLGTLLAYGMSGKMIVNNYLIVIFIIYFAAFVIGLVLALFFGLILYFTFS
                     VPIIYNYRVVLLVFIGILITLIYSRSIIFRIFNNTPGDLIYKR"
     gene            1871..2314
                     /locus_tag="PLG24_01480"
     CDS             1871..2314
                     /locus_tag="PLG24_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23581.1"
                     /translation="MKYLHVMLLLMILGLLACQKNKPSQEDIIAAEAEMDTIRKIITE
                     DSTISKAIIKDKKVEAKHKEDAERINKEIEKSPLKDLPCEKIIAEFRISVDKYCTGKI
                     TQEEMIASIPDLQDTKVKVCINNLFQEELDKINAKLEKCLESKGD"
     gene            2374..3066
                     /locus_tag="PLG24_01485"
     CDS             2374..3066
                     /locus_tag="PLG24_01485"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HMZ23582.1"
                     /translation="MDTIIFFNTKPEIKVAVEELVFSSQRPYTLYFPATYEQLGHLLV
                     KNTDALVFYSITAFTPDENMRLRYLLMNFNTVRFCLIGTVDLANLAWKLNVFHFEESP
                     VRYERLIFVYKKYVHQKTPLIDELIVKHNEEMLRIDYKRITHLSASGNYTLIHLNDGK
                     AILQTRQLGMFDFLTEKDLNFVRIHRSLMVNLRNISKIGNGKITFFGKENEIDISASL
                     ESKLKRTLLGRV"
     gene            3063..3737
                     /locus_tag="PLG24_01490"
     CDS             3063..3737
                     /locus_tag="PLG24_01490"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HMZ23583.1"
                     /translation="MKERIEISVGRDVSNDIVLNDPSVSLFHCTVSNETGGSVTISDL
                     NSANGTWVNNKRVVRKNITLDDKIGIGNFEPDPADFFKKIIELTDKRRKDFSEEFQEI
                     LRVFTIYQRKKDKITDTPKTGLILRLVFGVGLILILVFFKDLIPDFQTRYIIIMSIGL
                     LSFVSALFSAPQSKKNKMLDELRLEYEDKLVCPKCKSRLINHSLTYWQGKQQCPQPGC
                     DAVYHK"
     gene            3859..4083
                     /locus_tag="PLG24_01495"
     CDS             3859..4083
                     /locus_tag="PLG24_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23584.1"
                     /translation="MEDKNMKFKTNINCSGCIEKVTPFLDLSAGAGNWEVDTTNKDKI
                     LTVQGLDKSEAEIMDSVKRAGYRIEVLEAP"
     gene            complement(4262..4939)
                     /locus_tag="PLG24_01500"
     CDS             complement(4262..4939)
                     /locus_tag="PLG24_01500"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HMZ23585.1"
                     /translation="MKSEKFNLIFFLLTIVHLFAIEAEFGWLEVSTKPLLVAALMYWF
                     YINTALKNRFAQKVFAALIWSWMGDVLLMMVPINANFFLFGLGAFLIAQLAYASAFYE
                     DARLKGDRPVWLMPFAIAFFTTYCMLLFGYMAPKLGGLKIPVLLYAIVISIMAIMAWT
                     RYGTAKGRSYWLVALGAGLFVISDSVLAINKFSNPIPNAGIIIMLTYILAQYGITMGA
                     IARIRDR"
     gene            complement(4929..6146)
                     /locus_tag="PLG24_01505"
     CDS             complement(4929..6146)
                     /locus_tag="PLG24_01505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMZ23586.1"
                     /translation="MKVDYIALSIPIFFILIAIELAYTYSKKLRYFRLNDSLANLSQG
                     IGQQIIGVFMKTALFFCYLYIYEHWRFFTLPNTIWIWILLFFIVDFFYYWFHRKSHEI
                     NALWAAHVVHHQSEEYNLTVALRQSWFQGWFSWIFYLPVAFIGFNPIMFLTMTAFNTL
                     YQFWIHTRTIGNMGWLEYIFNTPSHHRVHHGSNPKYIDKNHGGTLIIWDRLFGTFQKE
                     EETVYYGITKPVNSWNPVWANFQNWADIVKLAGMMRTWKDKILVFINPPGWQPDYLGG
                     PLYPEEIDIENYSKYDKKSNVHLTAYSFFLFLIALAVGTALLFRFPQLNMLQCYATAA
                     FVMATLVSCGALFEQNFWLKAFEFSRILLSFLLVPLYLESSFSIIRWIFVAVQFISLA
                     WFISISKSIKSEV"
     gene            6273..6345
                     /locus_tag="PLG24_01510"
     tRNA            6273..6345
                     /locus_tag="PLG24_01510"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6306..6308,aa:Gly,seq:ccc)
     gene            7326..9041
                     /locus_tag="PLG24_01515"
     CDS             7326..9041
                     /locus_tag="PLG24_01515"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ23587.1"
                     /translation="MKFYSGFSTLIFLFTNLLAFGQSIPAFPGAEGFGASASGGRGGR
                     VIYVTNLNASGPGSFNDALSQTGPRYILFKVSGVIDAAAELREGDVTIAGQTSPGGII
                     TRGFLVDQVYEPGGSGDNIILRHLRSRPHDPEQVPSSHFILDDALRLDGAGNIIVDHC
                     SFANAIDECVQLSQSHDVTIQNCLLAETIGGHYGLGGMLINYSTAEHPQDRLSIHHNN
                     WNRIVGRLPEIICELPYCATAPLKLELSNNLVWDPHETSWYGANINPAGEDYRFFLEM
                     NWVGNLFYTRQAFPFAMMDIRLLQYAQNKVFASGNKMNLYPDYSDYQLCYCCNDFNQA
                     GSNPNTDFGIAQKLTTRHGFPSITYHSAADLPEYMVHNCGAFPRDQMDRRLMAPVSSG
                     TIAPEPVDGTDYFHDAFIVPASAPEPPADRDNDGMPDYWEELHGLDPDSQDHNGGQLS
                     LSVTGVAGYTNLECYLNCLSDMLVGGQSSSACGISLAEHSLPKKTQLMIYPNPAKGRF
                     TVHYGNVTNLEVEIVDFMGRKVYSGKCQGNACVIDAALPAGHYSVIARSEGKLVGLQK
                     VSIMH"
     gene            9242..9568
                     /locus_tag="PLG24_01520"
     CDS             9242..9568
                     /locus_tag="PLG24_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23588.1"
                     /translation="MLILIKTLHTIIWLFFNVVIFYIVYAVLTNRIDSMLWVCYGLII
                     LEGLTLAVFRLYCPLTVWARRFSTSDKPNFDIYLPEWLAKYNKLIYTSIVVGATVILL
                     FKMCSS"
     gene            11087..>14322
                     /locus_tag="PLG24_01525"
     CDS             11087..>14322
                     /locus_tag="PLG24_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HMZ23589.1"
                     /translation="MRKYLFVVAMLITSCLYSQVSDFQWQKPLAGINYDDTRSIEQTT
                     LVGFNVAGYNESIDGDAGGNHGYDDDRIGKLSLDCPDLSSAPDDVDIVNSTCGAGCTV
                     SGGSISAPVSGCPAGSTLQYSTDNGLTWSATIPTYNQTGPAQTIITRCNCDSDNGQSS
                     PSSAGVTTVPGTCTTPATPAITIVDNVCPSTTGTISATGCGAGTTLEWATNPSGPWST
                     ITPAYTETSFTVYARCIDDVTGCVGSVASATTAPTDCSGGDCSAIFISEYIEGSGNSK
                     ALEIYNPTASAVTLTGNASVKLYFNGSNTAGQTISLTGTIAAYGTFVLAHTSAAQDIL
                     DVANQTSGNLTFNGDDAVELIFDGNTVDVIGQIGFDPGTEWGSGNTSTADNTIRRKSS
                     VKKGDSDGSNAFDPSVEWDGFANNTFSGLGSHDSECNQAPSCPDLSSAPDDVDIVNST
                     CGAGCTVSGGSISAPVSGCPAGSTLQYSTDNGLTWSATIPTYNQTGPAQTIITRCNCD
                     SDNGQSSPSSAGVTTVPGTCTTPATPAITIVDNVCPSTTGTISATGCGAGTTLEWATN
                     PSGPWSTITPAYTETSFTVYARCIDDVTGCVGSVASATTAPTDCSGGDCSAIFISEYI
                     EGSGNSKALEIYNPTASAVTLTGNASVKLYFNGSNTAGQTISLTGTIAAYGTFVLAHT
                     SAAQDILDVANQTSGNLTFNGDDAVELIFDGNTVDVIGQIGFDPGTEWGSGNTSTADN
                     TIRRKSSVKKGDSDGSNAFDPSVEWDGFANNTFSGLGSHYSQCDPASPCPDLSAAPED
                     VTIVNSTCGTGCMSSGGSISPPASECPVGSTMQYSTDNGATWSTTLPVYNQNGPAQTI
                     ITRCNCDNDNTQSSPASSGVTTVPGLCPGPPAPTITIVDNVCPSTTGTISATGCDTGT
                     TLEWATNPLGPWSVTTPTYTNQAFTVYARCKDDISGCVSTVASATTAPTICPISDCST
                     IFFSEYVEGTSNNKALEIYNPSASTVTLSGNAIINIYANGGVTPSNTINLTGTIAPYG
                     TFVITHSSASASLLALADMTSNAMGYNGNDALELVYNGSAVDVIGQIG"
BASE COUNT         4308 a         2835 c         3156 g         4023 t
ORIGIN      
        1 gaattcatct ccccccggac atcttcaacc gaaatatcag gttgctttcc ggcggacagc
       61 gtcaaaggat atccttctta agggcactct tagccaacaa acctgttctc tttggcgatg
      121 aacctacggg taaccttgat ccaatcaccg ccaaaaccct tatgactatg gttcgcgaag
      181 atataagtcg ttccggaagt tctgcaatca ttgtttctca cgacctcaat cttgcccact
      241 tatttgctga caggattgtg acactaaagg tggatgaaaa cagacaatgt ggaattattg
      301 gcggcatctt cgaaagaaaa gacaatggct ggttggacca gttgtctcat aaaaatgtgg
      361 aacatgcaga aattactagc ttgctatgaa ggagatcaga agtattttta tcaaaaatga
      421 aataaagaag ctctttcaac atggcagaag ccattttatc ctgttgggta ttgtgttttc
      481 tctcctgatc atagcattga atcagtcaat atttggcctg aaagcattga atgaaaaata
      541 caacaaccca tttgccaatt atatacaggt agccactccc aagaataccg atttggacag
      601 agaaaccctc gccaaaacat tgattcaaaa attctcagcc gagcctttaa aggggaagct
      661 gggcatttca cgcatcaacg aacttagtta tgagttcatg cctttttgta atccggctat
      721 cgacaaagta aagacctata agggcagaac ttattccaag gatgatccct tatatcagga
      781 attggtgaag gcctcaggtg acaatatcat tacgactaac aaaatagaag aggatgatat
      841 caccgggcaa tatggagtaa tcattactga gaatatggtt cttgatctcg gttatgaccc
      901 ccggtcgatc aaaacggtac ccttaaagct cggcggaaag gaatttgttc ttgtccctgt
      961 catcgttgtg gtcaagtatc tgcctcagtt ttctgatttc atgattaccg aagacttctc
     1021 ctatatcaat aaccaaagta ttgaacagac caattttgta gatctgggta gtagcaattt
     1081 ttattcaata gcctccttca atgatacttc caaagtcctg gaggatgccc gaaaggtact
     1141 gaaaaaagat ttcaaggatg tgcaatgtaa gttgattgac atagatcaac tcaacaaggt
     1201 atcagtatgt gaattccagc tcaatgaata tttgtcggca gaggataagg aagatatgac
     1261 tgcctcactg aatacaggaa ataataagga caaactctac aacttttata cgctccacta
     1321 cgtaaatacg gaaaatgccg aaaaatttgc accggattat cttgaatttc agtttgcaag
     1381 cctggatagt atcaaggcat ttcaggaagt gtgcatgcaa aacagccttg atccggatat
     1441 gcaaattata aaatcgaggc atttattcgc tgaggtgggc tctatttcaa gaattgtttc
     1501 cggtggtttc atcctctttt cgattttgat gctctttatc tttaccaata gttttatttc
     1561 caggcatatt gaagagataa agcccaatct tggtaccttg ctggcttatg ggatgtcagg
     1621 caaaatgata gtaaataatt atttgattgt aatatttatc atatattttg cggcatttgt
     1681 catcggactg gtcctggcat tattttttgg cttgattctt tattttacat ttagtgttcc
     1741 aataatatat aattaccggg ttgtattgct tgtctttatc gggatcttga ttacccttat
     1801 ttattcaaga agcatcattt ttcgaatatt caacaataca ccgggcgact taatttataa
     1861 aagatagaaa atgaaatacc tgcatgtaat gctccttttg atgattttgg gccttttagc
     1921 ctgtcaaaaa aataagcctt cgcaagaaga tattattgct gccgaagcag aaatggatac
     1981 catccggaag attattactg aagatagtac catttccaaa gctatcatta aggacaaaaa
     2041 agtagaggca aaacacaaag aggatgcaga acgcatcaat aaagaaattg agaaatctcc
     2101 ccttaaagat ttgccttgcg aaaagataat cgcagaattt cgtatcagtg tagataaata
     2161 ttgcaccgga aagatcacac aggaagagat gatagcgagt atacctgact tacaggacac
     2221 caaagtaaag gtctgtatca ataacctctt tcaggaggaa ttggataaaa ttaatgccaa
     2281 actagagaaa tgccttgagt ccaaaggtga ttaagtaaga ttgtggatat taaacatgac
     2341 accggccagt aattgctgat atagtatttg tcaatggata ccattatatt ttttaacacg
     2401 aaaccggaaa taaaagttgc tgtggaggaa cttgtatttt cctcgcagcg cccttatacc
     2461 ttgtattttc ctgctactta tgagcagttg ggccatctgc ttgtaaaaaa tacggatgca
     2521 ttggtttttt attcaatcac agcgtttact ccagatgaga atatgcgcct gcgatacctg
     2581 ctcatgaatt tcaatacagt taggttttgt ttgattggta ccgtggacct ggccaatctt
     2641 gcctggaaat tgaatgtatt tcactttgag gaaagccctg taaggtatga aaggctgatc
     2701 ttcgtctata agaaatatgt tcatcaaaaa acacccctta tagatgaatt gattgtgaaa
     2761 cacaatgaag aaatgttgcg tatagactat aaacggataa cccatcttag tgcgtccgga
     2821 aactatacgc ttattcattt gaatgatgga aaagctatcc tccagacgcg ccagttgggt
     2881 atgtttgatt ttctgacgga aaaggatttg aacttcgtac gcattcaccg ctcgctcatg
     2941 gtcaatctca ggaatatttc aaagatagga aatggaaaga ttactttttt tgggaaggaa
     3001 aatgagattg atatttcggc gagtctggag agtaagttga aaagaacgct tttgggaaga
     3061 gtatgaagga acgaatagaa atatcggttg gtagagatgt atccaatgat attgtgctga
     3121 atgatccaag tgtaagcctc tttcactgta ctgtaagcaa tgaaaccgga ggatcggtca
     3181 ccatctctga cctcaattct gccaatggta catgggtcaa caacaaaagg gtcgtgagaa
     3241 aaaatataac cttggatgat aagattggca taggcaactt tgaaccggat ccggcagatt
     3301 ttttcaaaaa aattattgag ctgacagata aaagaagaaa agactttagc gaagaatttc
     3361 aggaaatttt gcgagtattc accatctatc agcgaaaaaa agataaaatc actgatactc
     3421 ccaagaccgg tctcatactg cgccttgtat ttggggtcgg tttgattttg attctggtct
     3481 tctttaagga tttgattcct gactttcaga cacggtatat tatcattatg tcgatcggat
     3541 tactgtcctt cgtctctgca ttattcagtg ccccccaaag caaaaagaat aaaatgctgg
     3601 acgaattaag actggaatat gaagataaat tggtgtgtcc taaatgcaaa tccaggctga
     3661 tcaatcatag cctgacttat tggcagggta agcaacaatg cccacaaccc ggatgtgatg
     3721 ccgtctatca caaataaaca actattttaa ccacatcgtt taccggctgc aatcactccc
     3781 tttcaaatat tttggggcaa aaacaaaccc ggaattttag tgtttatcat acatgcatga
     3841 ccttaatgag aaataactat ggaagataaa aatatgaagt ttaagaccaa catcaattgt
     3901 agcggatgta tagagaaagt gacacctttt cttgatctat ctgccggagc aggcaattgg
     3961 gaagtcgata caacgaataa ggacaagatc cttactgtgc aaggtctcga caagtctgaa
     4021 gctgaaataa tggatagcgt gaaaagagcc ggctacagga tcgaagtgct ggaagcgcca
     4081 taagacaggc gattttattc caagataatt tatataatag tccataaatt tttggtataa
     4141 tgcctaaagt acacaatcgg gtaccttcag tttgatttat aatataaatt ccgggaagcg
     4201 tacgacccgg accagtttcc cggaggatta catcgaaatc caccgttgaa cactattcgc
     4261 attacctgtc cctgatccgg gcaattgccc ccatcgttat gccatattgt gccaggatat
     4321 aggtgagcat gatgatgatg cctgcattgg gtatcgggtt ggaaaacttg ttgatggcaa
     4381 gaacggaatc ggatataacg aaaagcccgg ctcccaaagc tacaagccag tacgaacgtc
     4441 cctttgcggt gccataacgc gtccatgcca tgatagccat gatggaaata acgatggcgt
     4501 aaagcagaac aggaattttc agccctccaa gctttggggc catataaccg aacagtaaca
     4561 tgcaataggt ggtaaaaaat gcgatggcaa atggcatcaa ccagaccggt cggtcaccct
     4621 ttaaccttgc atcctcataa aaggcggatg cataagcaag ttgggcgatc aggaaagcac
     4681 ccagaccgaa cagaaagaag ttagcattga tcggtaccat catcagcaag acatcaccca
     4741 tccatgacca gatgagagcg gcgaatactt tttgtgcaaa tcggtttttt aatgcggtat
     4801 tgatatagaa ccaatacatc aacgcagcta ccaaaagggg cttcgtcgaa acttccagcc
     4861 acccgaactc tgcctctata gcaaaaaggt ggacgattgt cagtaagaaa aaaatcaggt
     4921 tgaatttttc agacttcact tttaatcgat tttgagattg agataaacca ggctaaagat
     4981 atgaattgca ccgcaacaaa gatccatctg atgatagaaa acgatgattc cagatacaat
     5041 ggcaccaata gaaaggaaag taggatccga ctgaattcaa acgccttcag ccagaaattt
     5101 tgctcaaata aagcaccaca gctgaccaat gtcgccatca caaaggctgc ggttgcatag
     5161 cattgtagca tgttgagctg tggaaaacga aacagcaggg ctgtaccgac ggccagggca
     5221 atcaggaaca gaaaaaagct atatgcagtg agatgtacat tagatttctt atcgtatttg
     5281 ctataatttt caatatctat ttcttccgga tatagaggac cgcccaggta atcaggttgc
     5341 cagccgggag gattgatgaa gacaagtatt ttatctttcc aggtgcgcat cattccggca
     5401 agcttgacaa tgtcagccca gttctgaaaa ttggcccata caggattcca gctgttgaca
     5461 ggcttggtaa ttccgtaata tactgtctct tcttcctttt ggaaggtccc aaacagtcgg
     5521 tcccagatga ttagtgtgcc tccgtgattc ttatcaatat actttgggtt ggagccgtgg
     5581 tgtacccggt gatgtgaagg cgtgttgaat atatattcca gccaacccat attgccgatg
     5641 gtgcgggtgt gaatccagaa ttgatacagg gtgttgaagg cagtcatcgt aagaaacatg
     5701 atgggattga accctatgaa tgcaacagga aggtagaaga tccagctaaa ccagccctga
     5761 aaccaggatt gtctcagcgc cacggtaaga ttgtattcct cagattgatg atggacgaca
     5821 tgtgctgccc acaaagcatt gatctcatgg ctttttctgt ggaaccaata gtagaaaaag
     5881 tcaacaatga aaaacaacaa tatccatatc cagatggtat tgggcaaggt aaaaaagcgc
     5941 caatgttcat agatatacag ataacagaaa aacaaagcag tcttcatgaa aacaccgatg
     6001 atttgctggc caataccctg actgagatta gcgagggaat cgttgagcct gaaatatcga
     6061 agttttttgg aataggtata ggcaagctca atagcaatca ggataaagaa gatgggtatt
     6121 gaaagtgcta tatagtcaac cttcatgata tttaactttg ttcaaaaata ggtaaaaaca
     6181 ttgaaaattt tcgttgggag aaaatgaatg agaatgatta aatttgacct gtcaatcatg
     6241 ccggcatcca tcggcgtttg attgagccct ctgcggaagt agctcacccg gtagagcgac
     6301 agcttcccaa gctgtaggta gcgggttcga gtcccgtctt ccgctcatct tttcaatgtt
     6361 gtgacaggta agtattcgtg cagcccatga agctttattc attgtttttg actaaatcaa
     6421 tatgcttatt atcaatttac cattgagatt attgtaaacc ctaattgaag tcttcaaggg
     6481 gtgaaaggca aaataaaaac atatgtattt aaatccagtt ctgtaggcac gacaatatac
     6541 gccggggagt cttatagggc attgtcctga aaaatcagct gtagggaaaa tgaaggggta
     6601 tagagaagtc attgatcctt ttacattatt tgctattagt caccagatag atggatcttt
     6661 ataaaaaaag gcagttgcga aaagcaactg ccctcaaagt gtgtgtactt tacccctttt
     6721 ggggtcgtgc gttttgtgtg tgaaccgcac catagtgcca caggattata atagttaaat
     6781 atggccgaaa tcgcaataag ctattcggaa attaaaaaaa agatgaatgc tttgacagac
     6841 ctattttagc cggaacaaaa attcgcaata agccaaattc tgactaaacc cgaattaaca
     6901 ttccttttct tcaggtatta cattaaataa cccgaattac tcaataaaga cgtaagtaat
     6961 gttgcaaggt cacccggaaa gtcagaaaat tttataaatt ttttgatctg aacaaatgta
     7021 taatatcggg aataggatat caggaatttt aactatctga ttttgtttgc gttatgccaa
     7081 gatgatttat ataatagtcc ataagttttt ggtatattgc cgaggttaca gtcgtataga
     7141 tcaatgaaat tggtctataa cgaatgtacc ttctgtatta cgtatgtgcg gtaggatgga
     7201 gacatcagca attgattatt tttatcgata tttccatgaa attggaaaga cccgaataaa
     7261 ttccgggtat gaataatata aaatcatgta gatattttaa atttgatgga ttaaatactg
     7321 acacgatgaa attctattcc ggtttttcta cattgatctt tctctttact aatttacttg
     7381 cattcggcca gagcataccc gcattccccg gagcggaggg ttttggggct tcagcatcag
     7441 gtgggcgagg tggacgtgtt atctatgtta ccaacctcaa tgcaagcgga ccgggtagtt
     7501 tcaatgatgc cctttcacaa accgggccta ggtatatttt atttaaggtc agcggtgtga
     7561 ttgatgctgc cgccgaactc cgcgagggag atgtcaccat cgcaggacaa acctcgccgg
     7621 gtggtatcat caccagaggg tttctggtcg atcaggtata tgaaccgggt ggctcaggtg
     7681 acaacatcat tctcaggcat ctgcgctcac gtcctcatga cccggaacag gttccttcct
     7741 cgcattttat cctggatgat gccctaaggc ttgatggtgc cggcaacatc atcgttgatc
     7801 attgctcctt tgccaatgcc atcgatgaat gtgtccaact atcccaatct catgatgtca
     7861 ccattcaaaa ctgccttttg gccgaaacca ttggagggca ctatggcttg ggtggtatgc
     7921 tgatcaatta ttctaccgct gagcaccctc aggatcgctt gagtatccat cataataact
     7981 ggaatcgtat tgtgggcagg ttgcccgaaa tcatctgtga gttgccttat tgtgccactg
     8041 ccccgttaaa gcttgagctt tccaataatc tcgtatggga cccgcatgag acatcctggt
     8101 atggagcaaa tatcaaccct gccggtgaag actatcgctt tttcctggaa atgaactggg
     8161 taggcaacct cttctatacc aggcaggctt ttccattcgc catgatggat atccgcctac
     8221 tccagtatgc tcagaacaag gtttttgcca gtggcaataa aatgaattta tatccggatt
     8281 attcagatta tcaactttgc tattgttgta atgatttcaa tcaggccgga tcgaatccta
     8341 atactgactt tgggatagcg caaaagttga caacaagaca tggttttcct tctattacct
     8401 atcacagtgc cgctgatctc ccggaatata tggtacacaa ttgcggtgca ttcccaagag
     8461 atcaaatgga cagaagactg atggcccctg tatcctccgg tacaattgct cccgagcccg
     8521 tggatggaac cgattacttt cacgatgcgt ttattgttcc tgcttcagct cccgaaccgc
     8581 ctgccgatcg ggataatgac ggcatgcccg attactggga agagttacat ggtctggacc
     8641 ctgactcgca ggatcacaat ggagggcaac tttccctttc cgtcaccgga gtagcaggat
     8701 ataccaatct ggaatgttac ctcaattgcc tgtcagatat gttagtcgga ggacaaagca
     8761 gttccgcctg tggcatttct ttggcagagc attcccttcc aaagaaaaca cagctcatga
     8821 tctaccccaa ccctgcaaaa ggcagattta cggttcacta cggtaatgta accaaccttg
     8881 aggtggagat agttgatttc atgggccgca aagtatattc gggcaaatgc cagggtaatg
     8941 cttgcgtgat cgatgcagcc ttgcctgccg gtcattatag cgtgattgca aggtcagagg
     9001 gcaagttggt cggtcttcaa aaagtgtcca taatgcacta attgaagata ggataaaggt
     9061 aaagaaaaca tttgcctcaa ttgattttga gctacaggat agggtgctaa ttattgagat
     9121 gaaagcacta cgataattgg gttatatttg tttaaatcag cagtaataaa gataaccaac
     9181 caggtaattg aggtcaggta aaagccggtt gacatcaata tgtatggaaa agggtagaat
     9241 aatgttgatc ctgataaaaa cactgcacac catcatctgg ttgtttttca atgttgtgat
     9301 tttctatatt gtgtatgctg tgcttaccaa tagaatagac agtatgcttt gggtatgtta
     9361 tgggttgatc atccttgagg gcttgacact tgcggtattc cggctatatt gtcccttgac
     9421 tgtatgggcg aggcgatttt ctacttctga caagcccaat tttgatattt atcttcccga
     9481 atggctggct aaatacaaca aactaatcta tacttcaata gtggtgggtg cgaccgtgat
     9541 tcttttattc aaaatgtgct caagttaagg tgttttgaca cctatgaatt ttcataaata
     9601 ttttgccagg caaagtatat tcatttcaac ctattcagaa gaatcagacc ggactgatgc
     9661 accaccactt tcaggagcaa agccggttaa gtataagaat tattttatca atgtaataaa
     9721 ctttacaaat tttatcatga ttttttccaa cctattaatc catcattacg tctttaatta
     9781 ttgaataagc agaaagattg cctgcagggc acatgatcat aacttttgaa gccccatttg
     9841 gaaggaaaga taaggtagga acgaatagca tagaatttaa tacatgaaag tcgtcgggtt
     9901 tttatccggc gacttttttt agatgggcta agcccgacaa tatgcgcaca aatatttttg
     9961 ctaagcccgt gaagtgttgc catacaagag gaggatcgta aagtatcagc ctacaaagat
    10021 atttttgcac cacaacaaat gtgttcaatc cactggtcag attcctgcca gattgagttc
    10081 attttttagc agagcctttg agtcctactg atgcggaaaa agaaagaaat ttgaaagatc
    10141 aatatggtat tgatgatttg gagagtccat aaaattttaa ctgtcaccat gacttgttga
    10201 tttgttttgc cggaccggag gtagtgactg atacactgag tcagatacgt agctgtgtaa
    10261 atatgggaat gcagcaagaa gatattgtgg gattaggata aatcaagttg ggccgttatt
    10321 tgatttgatg tttctgaata gtgcatgatt attcatttgt tttggaacag ccgacctttc
    10381 tcaggaactt caaaaaaccg ctacatatgc agatcgctgg acagataggt gcttcagaaa
    10441 atatcctgat cagaaaatgc cattaggatt gaggtgattt ttcagttgta attgtttgaa
    10501 attgttgaaa gtattcgact ttttcctgat gtcgaaaaac ttaacattgt ctgtataaat
    10561 gattagttgt ctgaaaacga aatgatcaaa gtaagatgta attttacata tgaaaaattc
    10621 cgggatatta attacaactg tgtaggataa tccaaatcat actgctattt ttgctttttt
    10681 cggataataa aaatattgaa attgataagt tgtaaaaaaa aacctggcaa tatgactttg
    10741 ctttgcaaaa aaacaattta atttaaccct aattttcaaa cactttgcaa tttaattgga
    10801 taattgtttg aattgagaga tcatatttat cgtgcaaaaa cctaacttat atcggacatt
    10861 tcaacaaatt acatatgttt ttgatcttga tgttcgcctt ttctaccggg tcacggacac
    10921 aatattcttt cggtttcggt ggatgcgaag gattattttg cattgggtgg ataatgtctg
    10981 gcggaatgaa ccaaatgaag cattagaaag ggattgtctg aagtcttgta actattgcat
    11041 taaatatttg tagaacattg atgtatcaac taataaaccc taatttatga gaaaatattt
    11101 atttgtcgtg gcaatgctca tcacctcctg cctttattct caggtttcag atttccaatg
    11161 gcaaaaacct ttggctggaa ttaattatga tgataccagg tctattgaac aaacgacctt
    11221 agtaggcttt aatgtcgcag gatacaatga atccattgac ggagatgccg gcggaaatca
    11281 tggttatgat gatgaccgga ttggaaaact tagccttgat tgtcctgatc tttcatcagc
    11341 tcctgatgat gtagacatag tcaacagtac ctgtggtgca ggatgtacgg tttcaggagg
    11401 tagcatatcc gcacctgtga gtggatgtcc ggcaggaagc acactgcaat attctacaga
    11461 caatggattg acatggagtg caaccatacc gacctacaat cagacgggac cggcgcagac
    11521 catcatcacc aggtgtaact gtgacagtga taatgggcaa tcaagcccgt catcggccgg
    11581 agtaactact gttccgggta catgtacgac accagccaca cctgccataa cgattgtgga
    11641 caatgtatgt ccatcaacaa caggtaccat aagtgctaca ggttgtggtg cagggactac
    11701 actggagtgg gcgactaacc catccgggcc gtggagcaca atcacgcctg cttatacaga
    11761 gacatcattt actgtatatg caaggtgtat tgatgatgtt accggttgtg taggatctgt
    11821 tgcaagtgcc actacggcac cgactgattg ttccggaggt gattgttcgg caattttcat
    11881 ttcagagtat attgaaggtt ctggcaatag taaggcatta gagatatata acccgactgc
    11941 atccgcagtt accttgacgg gcaatgcatc ggtcaagctg tacttcaacg gcagtaatac
    12001 agccggtcag accatcagtc tgacaggcac catcgcggct tatggaacat tcgtacttgc
    12061 ccatacaagt gcggcacagg atattctgga tgttgccaat cagacatcag gcaatcttac
    12121 tttcaatgga gacgatgccg ttgagttgat atttgacgga aacacagtgg atgtcatcgg
    12181 ccagatagga tttgatccgg ggaccgaatg gggaagcggc aatacttcga cagcggacaa
    12241 taccatacgc aggaagtcat cggtaaaaaa gggagattct gatggtagta atgcatttga
    12301 tccttcggtt gagtgggacg gttttgccaa taatactttt agtggattgg gaagccatga
    12361 ctcagaatgt aatcaggcac cttcttgtcc ggatctttca tcagctcctg atgatgttga
    12421 catagtcaac agtacctgtg gtgcaggatg tacggtttcg ggaggtagca tatccgcacc
    12481 tgtgagtgga tgtccggcag gaagcacact tcaatattct acagacaatg gattgacatg
    12541 gagtgcaacc attccgacct acaatcagac aggaccggcg cagaccatca tcaccaggtg
    12601 taactgtgac agtgataatg ggcaatcaag cccgtcatcg gccggagtaa ctactgttcc
    12661 gggtacatgt acgacaccag ccacacctgc cataacgatt gtggacaatg tatgtccatc
    12721 aacaacaggt accataagtg ctacaggttg tggtgcaggg actacactgg agtgggcgac
    12781 taacccatcc gggccgtgga gcacaatcac gcctgcttat acagagacat catttactgt
    12841 atatgcaagg tgtattgatg atgttaccgg ttgtgtagga tctgttgcaa gtgccactac
    12901 ggcaccgact gattgttccg gaggtgattg ttcggcaatt ttcatttcag agtatattga
    12961 aggttctggc aatagtaagg cattagagat atataacccg actgcatccg cagttacctt
    13021 gacgggcaat gcatcggtca agctgtactt caacggcagt aatacagccg gtcagaccat
    13081 cagtctgaca ggcaccatcg cggcttatgg aacattcgta cttgcccata caagtgcggc
    13141 acaggatatt ctggatgttg ccaatcagac atcaggcaat cttactttca atggagacga
    13201 tgccgttgag ttgatatttg acggaaacac agtggatgtc atcggccaga taggatttga
    13261 tccggggacc gaatggggaa gcggcaatac ttcgacagcg gacaatacca tacgcaggaa
    13321 gtcatcggta aaaaagggag attctgatgg tagtaatgct tttgatcctt cagttgagtg
    13381 ggacggtttt gccaataata cttttagtgg attgggaagt cactattcgc aatgtgatcc
    13441 ggcttcgcca tgcccggatc tttcagctgc accggaagat gtaacaatag tgaatagtac
    13501 ttgtgggaca ggttgtatgt catctggagg aagcatctcc cctcctgcct ctgaatgtcc
    13561 tgtaggaagc acaatgcaat attctacaga taatggagcg acatggagta ctacacttcc
    13621 agtctacaat caaaatggac cggctcaaac cattattacg cgatgtaatt gcgacaatga
    13681 taatacacaa tcaagccctg catcatcagg agtgacaact gtgccgggtc tttgtcccgg
    13741 tccaccggct ccgacaatta caatcgtgga caatgtgtgt ccatcaacca cagggactat
    13801 cagtgctaca ggttgtgaca ccgggacgac cttagaatgg gccactaacc cacttggccc
    13861 atggagtgtg accacaccca cctacacaaa tcaagcattt acggtgtatg caagatgtaa
    13921 ggatgatatt tcaggatgtg tcagtacggt tgcaagtgct acaacagcgc ctaccatatg
    13981 tccgataagt gattgctcga ctattttctt ttctgaatat gttgaaggaa catcgaataa
    14041 taaggcgctt gaaatatata acccgtctgc ttcaaccgtc accctgtcag ggaatgctat
    14101 catcaatatc tatgctaatg gtggcgttac gccaagcaac accatcaatc taacaggaac
    14161 gattgctcct tatgggacat ttgtcataac ccatagcagt gcatctgctt cgttgctggc
    14221 gcttgctgac atgacttcaa atgccatggg atataatgga aatgatgcat tagaactggt
    14281 gtacaatggc agtgcagtag atgtcatcgg tcagattggc tt
//
LOCUS       DAOQQN010000022        14024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1444_length_14024_cov_5.257857, whole genome shotgun sequence.
ACCESSION   DAOQQN010000022 DAOQQN010000000
VERSION     DAOQQN010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14024
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1444_length_14024_cov_5.257857"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="PLG24_01530"
     CDS             complement(<1..780)
                     /locus_tag="PLG24_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HMZ23590.1"
                     /translation="MEGIRSTLYNLPDGVTRYEGKVRDVFTRDNIFVSVASDRISAFD
                     HILPRSIPYKGQVLNQIAAYFLDSVRDIIPVWLDSTPDPNVSVGRKCTPIRIEMVVRG
                     YLSGHSWREYRSGKRFLCGVPLPEGLMENDPFPEPIITPSTKAMEGHDEDISKEEILE
                     RNLVDRQIYEEMEIKSRQLFERGTQMALKRGLILVDTKYEFGIYNGALTLMDELHTPD
                     SSRYFYLDTYFENQILHRPQNQLSKEFVREWLIANNFMGKEG"
     gene            complement(859..1299)
                     /locus_tag="PLG24_01535"
     CDS             complement(859..1299)
                     /locus_tag="PLG24_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23591.1"
                     /translation="MSIFDKAVLVIYRMAEKGLEVFLVKSEDEEIQSQLVEISEKFLT
                     DLPLRFSDNSKMIELDPIEDENMEKVRAVAIEGDWHDIPSLRAMLREDLQFMKVKMKK
                     YIPTIDSGNFILFKDAVKKAVRSEYRLLKELKDVLTDRNTINNI"
     gene            1780..6393
                     /locus_tag="PLG24_01540"
     CDS             1780..6393
                     /locus_tag="PLG24_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ23592.1"
                     /translation="MNLRLLHILLFISISFVTNLDGQKLKSVGNQSEQNIEIRDCSTR
                     SPGTVSFGPIVGQSIDGRPDTIYLCYKDQIFIKNNGDGNFSTGDPNPGTPSAIGYLLY
                     GAPPTINGPDFNAIQGDAVLTNPPPPPGELWYFMDAPNGDITLYNDGSVNETFNSGKP
                     FPLYLAPVTFDAYYAAGGEYYGQWENGGSCVKVNTAAAFPVVYLNEIKINNFATNSPN
                     SLSGSFNVTGGFPEFRNGSTYSISMVKKGAPNVVANLFGGPFKHGDLVQFAVPSGGTY
                     ILTISDGKSCSVTKEIIMPQGNVSVLVQSGTVDLNDTICLEFTVKDFKDLLGGEFAIT
                     FNPLELKYVGLNFPQTNPLNLSPGGNFGLTEASNGYIVFAWTDANLNKKTLADGTVIF
                     SICFQAIGRPGTKTPVKVSPKRDGVIEFTDYDGTLYTVRTVDGIVTINNPTKLQVFFN
                     VCSTTGNTGSVTFTAYGPDAQYNYEFNNSGAPDFTNAGTPVTFSNLTPGPYKIDVYSI
                     SGVHVVHTVFIQNAPPINITGNLTNPTCFDTSDGNVQISLQGGVPPYAIEWSNFTFNT
                     NTVKDVGNGLYTVTVTDDIGCIFSQSFTLYTSPISINPPAVTDPACSGQKNGSITISA
                     SGGNPKTGNTYDFHWSYQNTSQIGVSIGSLTNIPEGTYYVTVTDNNGCNATSSFVVDP
                     GVVIDATVLKQLPVCFGDGKGRLTVTGTTNGTTSGPYTFIWSPNTGNVSSTSTTSIAS
                     GLNNGNYIVTVEDANGCFVKKELEINGPGEIKFLIANLKDDVCGTQPAGSALIYDGSG
                     NVTPFPDPNDPDNGFYRFTWSNGSNPTTVDAVSLNLTNLAGSASGTRYYVTITGADGC
                     TADTSFLIFKKDAPMITFDTLHSVSCAGGSDGSIRANITANGSDISTVTWSNNAGVPV
                     NSGDPKNVYTSTVNNLNGGQYIVTVVTLSGCIVTDTINLESAGSLVLDNNFIVPATCP
                     DSDDGSITLVMSGGNPPYNYQWSNGQTGDVASNLKPGNYTVTITDQSACPPVVQTFTV
                     GNAPTVLSAIDVNSVVASDCFESTAGTGGATVQASGGQANSYTFTWSSGETNTGVSST
                     ASQLKGGWQYVTITDGFCQIIDSVFIPSPDPIISNKSGATIKDVSCYGLTDGSLSFNP
                     TGGNGGYNYSWNPTATNSSSLSGLTAGTYHVTITDSKNCIGIDSFQIGQPVELIASLD
                     TILTKDIACHGEQSGSIKVNVQGGNGGNTYTWNPAVTGNSDLANNLAAGNYLITVIDI
                     KGCQSSIAAVIAEPTPLSVDFNPVPDPACAGYETNFELNNITGGVGPDYSYTIDHGKS
                     YSTFEIAKVTGGVHILTIIDENGCRKDTTFTVNEPAPILVSLPEKLQMNLGDTLRLSP
                     EVQSAFPINNITWSPAGSVSCVTCDYTFASVITSQYITAVATDVNGCTGQDSVFLMVD
                     RSRKVFIPNAFSPNKDGINDIFQVFTGPGVEGINYIRVYDRYGALVYEINNLPPNENG
                     SSQGWDGTHKGKYCDPGVFTYLSEVKFIDGRTQKYFGSVTLVR"
     gene            complement(6516..6986)
                     /locus_tag="PLG24_01545"
     CDS             complement(6516..6986)
                     /locus_tag="PLG24_01545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HMZ23593.1"
                     /translation="MKILKKFVLIAPILLLIACEKDPSSVDDSAQLEIDKQKIRAYLN
                     QYNIKADSTSEGLYYVIDTKSDTSTRKPTSTSKVKVDYKGYLLNGKVFDNGTGVSFFL
                     TSLIPAWQIGLPKFNEGDKGRLFVPSKLGYGKSGSGSIPANSPLIFDITLIKVD"
     gene            complement(7049..8482)
                     /locus_tag="PLG24_01550"
     CDS             complement(7049..8482)
                     /locus_tag="PLG24_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HMZ23594.1"
                     /translation="MFLLLNRSENEEFLYKILARAASDLDLQTYLVGGYVRDLILGRN
                     STDIDVVCVGDGIQLAEQVNKLLGNKQPIVVYKNFGTAALRYNDIQIEFVGARKESYQ
                     KNSRKPAVIAGTLYDDQLRRDFTINALAISLNTDTYGELLDPFDGMEHLEEKLIKTPL
                     DPEDTFSDDPLRMMRAIRFSNQLNFSIDPLTYAAINHNKDRLKIVSIERIVTEFQKIM
                     ACDKPSIGIKLLFDTGLLEVFLPELVELQGVEYQDGKGHKDNFYHTLEVLDNVCRNSD
                     NIWLRWAALLHDIAKPATKRFDEKQGWTFHGHDALGAVMVYKIFKKLRMPLDQHVKYV
                     QKLVRYHLRPISLTKEDITDSAIRRLLFDAGDEIDDLMILCEADITSKNEKKVKRLLE
                     NYEMVRLKLKEIEDKDRIRNWQPPVSGELIMETFDLKSGPIVGELKNEIREAILEGDL
                     ENEYNAAYHFLLELGAKLGLERKSSAQ"
     gene            complement(8494..9489)
                     /gene="dusB"
                     /locus_tag="PLG24_01555"
     CDS             complement(8494..9489)
                     /gene="dusB"
                     /locus_tag="PLG24_01555"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HMZ23595.1"
                     /translation="MVKIGGISLGEFPLLLAPMEDVSDPPFRALCKAQGCDMLYTEFV
                     SVEGLIRNADKSVQKLDISDEERPVGIQIFGSEKESMIQAAEIIERTQPEVLDINYGC
                     PVKKVVCKMAGAGILQDIDRMVSLTEAVVKATSLPVTVKTRLGWDEDSKNIVEVAERL
                     QDIGIKALSIHGRTRKQMYTGEADWTLIAKVKENPRIHIPVFGNGDIDSPEKAKLYKE
                     RYGVDGIMIGRASIGNPWIFREIKQYLKDGTIPDPPSLDERIEAVLQHLHRSIMWKGD
                     KVGINEMKRHYGNYFRGYREVKQFRTLLVHSSSFDEVVEITEKMREYYAGEELTV"
     gene            complement(9670..12345)
                     /locus_tag="PLG24_01560"
     CDS             complement(9670..12345)
                     /locus_tag="PLG24_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ23596.1"
                     /translation="MTLDNFTINAQDAIIKAQKIASDRSQQTVDTAHILRGIIDTDES
                     VVSFLLNKMAVDLNPIKDENDKLIDLIPKVQGSDKQFLSNDANNAISKAKEAMKEFGD
                     EFISLELLFLGLFKGSDRTARLLKDRGLTEAGLVSAIKELRKGNKVNSQSGDSSYNSL
                     GKYAINLNERAESGKLDPIIGRDEEIRRVMHILSRRKKNNPILVGEAGVGKTAIVEGI
                     AWRIVKQDVPENLKSKTIFSLDLSSLVAGAKYKGEFEERLKAVIKEVTESDGEIILFI
                     DEIHTLIGAGGGSGAMDAANILKPALARGELHAIGATTLDEYQKYFEKDAALVRRFQN
                     VMVDEPSVEDTISILRGLQEKYEVFHKIEILDEALIAAAEMSHRYIADRQLPDKAIDL
                     IDEAAAKLRLELNSMPDSIDELDRKVRQLEIEREAIKREKDDKKLAAISESIANAKQQ
                     LDSVMAKWKNEKAIVDRINTLREEIEELDRKAQKAERESDFETVAKIRYGELQAKQQE
                     LKESENELNALPEGSRFTAQMVTANDIADVVSKWTGIPVSKLQQSEKDKLLNMEKEIG
                     KMLIGQTEAVEAVSEAVRRNRAGLSDSKRPVGSFIFMGPTGVGKTELTKALADVLFDD
                     KDAIIRIDMSEYMEKHSVSRLVGAPPGYVGYEEGGQLTEAVRHRPYSIVLLDEIEKAN
                     ADVFNILLQVLDDGRLTDGKGREVNFKNTIIIMTSNLGSNIILENFEDLEEIEPKRRS
                     EVIETTKLEVFELLKQTMRPEFLNRIDETIMFLPLTKEEIGKILDIQMKGINKLLKSQ
                     ELRIELAPSASKLLIDLGYDPQFGARPLKRVLQKELVNELSKQLLSGDYLPGDVIVAE
                     GNNTGLTFHKKKEVMTEPVTENHTDHTDSGRSEEK"
     gene            complement(12879..13253)
                     /locus_tag="PLG24_01565"
     CDS             complement(12879..13253)
                     /locus_tag="PLG24_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HMZ23597.1"
                     /translation="MSIGIMIAKTLVAIVAIQHLFFMWLEMFAWETVGRKFFKGNRQG
                     DFFRQTKSIAANQGLYNGFLAAGLIWSFLISDSLWQINVMTFFLGCVVVAGIFGGYSV
                     SRKIFLVQAIPAMLALIFLHLL"
     gene            13741..>14024
                     /locus_tag="PLG24_01570"
     CDS             13741..>14024
                     /locus_tag="PLG24_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HMZ23598.1"
                     /translation="MADQNFIDYVKICCRSGKGGAGSHHYFRDKMNSKGGPDGGDGGR
                     GGHIILKGNRNVWTLINLKYRKHVIAEDGVNGSKNNRTGRSGKDVILEVPL"
BASE COUNT         4058 a         3151 c         2657 g         4158 t
ORIGIN      
        1 tccttcttta cccatgaaat tattggcgat gagccattct cttacaaatt ccttggaaag
       61 ctggttctga ggccgatgaa gaatttggtt ttcaaagtat gtatccagat aaaaatacct
      121 ggaactgtca ggtgtgtgca attcatccat cagagttaat gcgccattat atatcccgaa
      181 ttcatatttt gtatccacaa ggatcaaacc acgcttcaaa gccatttggg tccctcgctc
      241 aaacaactgg cgggatttaa tctccatttc ctcatagatt tgacgatcta cgagattcct
      301 ttccagaatt tcttcttttg aaatatcttc atcgtgaccc tccattgcct tggtacttgg
      361 tgtaatgata ggctcaggaa aagggtcatt ttccatcaag ccctccggca ggggaactcc
      421 gcaaagaaat cttttaccac ttctatattc tctccatgaa tgaccactga gatagccacg
      481 aactaccatc tcaatcctaa tcggtgtgca cttccttccg acagaaacat tcggatcagg
      541 ggtggagtcc agccagaccg gaatgatatc tcgaacagag tccaggaaat aagctgcaat
      601 ctgattgagg acctgacctt tgtaaggaat agacctcgga agtatatgat caaatgcaga
      661 aattcggtca gaagcaaccg aaacaaaaat gttatctctg gtaaatacat cccttacttt
      721 accctcatat ctggtgacgc catcgggtaa attatataat gtacttctta tcccttccat
      781 aaaaataaat tatctgatcc ataatcgaca tcagccaaga taggctgatt gtacactgac
      841 atcagatcta cttaatgtct atatattatt gatggtattc ctgtcggtca ggacatcttt
      901 gagttccttc aagagtcggt attcggatct aacggctttt ttaaccgcat ccttgaataa
      961 aataaagtta cctgaatcaa tcgtcggtat atattttttc atcttgacct tcataaattg
     1021 aagatcttca cgaagcattg ctctaagtga tggaatatca tgccaatcac cttcaattgc
     1081 aaccgcccgt actttctcca tattttcatc ttcaatagga tccagctcaa tcatcttgga
     1141 gttgtcagaa aagcgcaaag gcaagtccgt caggaatttc tctgaaatct ctaccaattg
     1201 agattgtatc tcttcatctt ccgattttac caaaaaaact tccaaacctt tctcggccat
     1261 ccggtatatt accaaaacag ccttgtcaaa tatcgacata tcaactattt gaaaaattat
     1321 aatacaaagc taaacattaa taacatataa caacaaatat attttgattt ttcatcacta
     1381 tcctaatcaa atcttttgaa tctataaagt ccaggcttaa ctaaccctta aataaaaaag
     1441 taatatctca aaccgaatta gagctatgag tggcttaaaa aatcaatgaa aatctaataa
     1501 aaattgcatt catggttttt ctgtattttg gtcgaataca ggatttaaat acgatggcac
     1561 ccatataatt cgttaaattt gtaaataatt tttgtttccc aaaaaaagaa aaacaaatat
     1621 taaaaatata tatattaatt ttgcatcaca tatgaaagtt gcattcaaga ttgcagctaa
     1681 ttgataataa cagatatata tttaataatg atcaatttgg gtttttactt taaacatgcc
     1741 cagaaatcag gacgaactca acaacacaga atattttgta tgaacttaag attactacac
     1801 atccttctat ttataagcat tagtttcgtc acgaatttag atgggcaaaa gcttaagtct
     1861 gtcgggaatc aatcggagca gaatatcgag atcagggatt gttcgacaag gagccctggc
     1921 actgtaagct tcggacctat tgtcggtcaa agtattgatg gaaggcctga cacgatatat
     1981 ctttgttaca aggatcagat ttttataaaa aacaatggcg atggaaactt tagcaccggt
     2041 gaccctaacc caggcactcc ttcagcaatc ggatatttgt tgtatggtgc gcctcctact
     2101 ataaatggtc ctgacttcaa tgcaatacag ggagatgcgg tcttaaccaa tccaccaccg
     2161 ccacccgggg aactatggta ttttatggat gcaccgaatg gtgacatcac cttgtacaat
     2221 gatggttcgg tcaatgaaac cttcaactca ggcaagccat ttcctttata tctggcaccg
     2281 gtaacctttg atgcatacta tgcagcgggt ggagaatatt acggccaatg ggaaaatggg
     2341 ggttcatgtg taaaggtaaa tactgcagcc gcctttccgg tggtgtatct caatgaaata
     2401 aaaatcaaca atttcgctac caacagtccg aactccctat ccggctcctt taatgtgacc
     2461 ggtggatttc ctgaattcag gaatggctcg acatattcga tttcaatggt taagaaagga
     2521 gcgcccaatg tagtggcaaa tctttttggt ggtccattta agcatgggga tttggtacaa
     2581 tttgccgttc cgagtggcgg cacttatata ctgactattt cagacggaaa atcctgttca
     2641 gtcacgaaag aaattatcat gccacaaggc aatgtatcag ttttggtaca atccggcaca
     2701 gtagacctga atgacaccat ttgcctggag tttaccgtta aagatttcaa ggatctgtta
     2761 ggcggggaat ttgccatcac tttcaatccc ctggagttaa aatatgttgg tttaaatttc
     2821 ccccagacca atcctctgaa tttaagtcca ggaggaaatt ttggtctcac ggaagccagc
     2881 aatggttata tcgtatttgc atggacagat gccaatctga ataaaaagac cctggctgat
     2941 ggtaccgtta tcttttccat ttgctttcaa gctattggca ggccaggaac gaaaaccccg
     3001 gtcaaggttt cacccaaacg agacggtgta attgagttta cagattatga cggcacgctc
     3061 tatacggtaa ggactgtaga tgggatagtt acgatcaata atcccaccaa actgcaggta
     3121 ttcttcaatg tgtgcagtac aacgggcaac accggttctg ttacgttcac tgcatatgga
     3181 cctgatgcac aatacaatta tgaatttaac aacagtggag caccagactt tacaaacgca
     3241 ggtacacctg taacattcag caaccttaca ccgggaccat ataagataga tgtatattca
     3301 atcagtgggg tacatgttgt acacactgta ttcatacaaa atgctccacc aatcaacatt
     3361 acaggtaacc taacgaaccc tacctgcttc gacacttctg atggcaatgt ccagattagt
     3421 ctacagggtg gagtaccacc ttatgcgatt gagtggtcta attttacctt taataccaac
     3481 accgtgaagg atgtcgggaa tggactttac acagtcactg ttacagatga tatcggttgt
     3541 atattttccc aatcctttac actttatact tctcccatca gtattaatcc tccggcggta
     3601 actgatccgg cctgttcagg tcagaagaat gggtcgatta ccatttctgc ctcgggaggt
     3661 aatccaaaga caggcaatac ctatgatttt cattggtcat accagaatac cagtcagatt
     3721 ggggtatcaa taggctcatt gacaaatatt cctgaaggga cctattacgt tacggtaaca
     3781 gataataacg ggtgtaatgc cacctcgtca tttgtggtag atccgggcgt tgttattgat
     3841 gccacagtac tcaagcaact tcctgtatgc tttggtgacg gcaaaggcag acttactgtc
     3901 acaggtacga ccaatggcac gacctcaggc ccttacactt ttatatggag ccccaatacc
     3961 ggtaatgtct caagtacctc aacaacatct atagcaagtg gactaaacaa tggcaactac
     4021 attgttactg tagaagatgc aaatggctgc tttgtcaaaa aggaacttga aattaatgga
     4081 cccggggaaa taaagtttct gattgccaat cttaaagatg atgtgtgtgg cacacaacct
     4141 gcaggttctg ctttgatata cgacggcagt ggcaatgtaa cgccatttcc tgaccctaat
     4201 gatcctgaca atggatttta cagattcaca tggtctaacg gttctaaccc gacgacagtc
     4261 gatgcagtaa gtctcaatct aactaacctt gcaggaagtg cttccgggac aagatactat
     4321 gtgactataa caggtgcgga tggttgtacg gctgacacca gttttttgat tttcaaaaag
     4381 gatgcaccta tgattacttt tgatacgttg cactccgttt catgtgcggg tggtagtgac
     4441 ggatcgataa gagccaacat taccgccaat ggttcagata tctccaccgt aacctggagt
     4501 aacaatgccg gtgttcctgt caatagcgga gatccaaaga atgtttatac ctcgacagtc
     4561 aataatctta atggaggcca atacatcgtc actgtggtta ctttaagcgg atgtattgta
     4621 accgatacca tcaatctgga gagtgcaggg tctttggtac tggataataa tttcattgtt
     4681 ccggcaacat gcccggactc ggacgatggt tctataactc tcgtaatgtc aggcggtaat
     4741 cctccgtata actatcaatg gagtaatggg caaacaggtg atgtcgcctc aaatttaaaa
     4801 ccaggcaact acactgtgac gattacagat caatcagcct gcccccctgt cgtccaaact
     4861 tttactgtgg gcaatgcacc tacagtactt tcggccatag atgtcaactc tgtagttgca
     4921 tcggattgct ttgaatcaac ggcaggaacc gggggagcta ctgttcaggc atccggaggt
     4981 caggccaatt cttatacttt cacatggtcc agcggagaga caaacactgg tgtatcatcg
     5041 actgcctcac aactaaaagg cggctggcaa tatgtaacga ttactgatgg attttgccaa
     5101 atcattgatt cggttttcat tccgtctccg gatccaatca tttcaaataa atcaggtgca
     5161 accatcaaag atgtttcatg ttatggcctg accgatggtt ccttgtcatt taatccaaca
     5221 ggagggaatg gtggatataa ttattcctgg aacccaactg ccaccaactc atccagcttg
     5281 tccggtctga ctgccggaac ctatcacgta accatcaccg actcaaagaa ttgtatcggt
     5341 attgattcat tccaaatagg tcagcctgtc gaattgattg cgtcccttga taccattttg
     5401 acaaaggata ttgcttgtca cggtgagcaa agcggttcga ttaaggtaaa tgtacagggt
     5461 ggaaatggtg gaaatacata cacttggaat ccggcagtga cgggaaattc ggaccttgct
     5521 aacaatctgg ctgcaggaaa ttacctgatt actgttatag atataaaagg ttgccaatcc
     5581 agtatagcag cagtaatcgc agaaccgact cctttatccg ttgattttaa tccggttcct
     5641 gatccggctt gtgccggata cgaaaccaac tttgaattaa ataacatcac cggtggcgtc
     5701 ggaccggatt actcctatac tatagatcat ggtaagagtt attctacttt tgagattgca
     5761 aaagtaacag gaggtgttca catactgacc atcatcgatg agaacggatg tagaaaggac
     5821 accacattta cagtcaacga gcctgcaccc atccttgtaa gtcttcctga aaaattacag
     5881 atgaacctgg gtgatacgct cagactcagc cctgaagttc aaagtgcatt tccaatcaac
     5941 aacattacat ggtctcctgc aggatctgta tcttgtgtaa cctgtgatta tacctttgcc
     6001 agcgttataa ccagtcaata cataacagca gtcgctaccg atgtaaatgg atgtactggt
     6061 caagattccg tattcctaat ggttgatagg tcaaggaaag tatttatccc caatgctttc
     6121 tctcctaaca aagatggcat caatgatatt ttccaggttt tcaccggccc gggcgtagaa
     6181 ggaatcaact acatcagggt gtatgaccga tacggcgccc tggtatatga gatcaataat
     6241 ctccctccaa atgagaacgg atcaagtcag ggttgggacg gaactcataa aggaaagtat
     6301 tgcgatccgg gcgtatttac ctatttgtcc gaagttaagt ttatcgacgg caggacacaa
     6361 aagtatttcg gcagtgtgac ccttgtccgg tagtgaagca ttatatatca ggaaaaacaa
     6421 taataaagaa aaaccgaaca gcattttgtt gttcggtttt ttgttaagca ttaaaccatg
     6481 cttactatat aaatttatta ctgaaaaaga attactcaat ccaccttaat cagcgtaatg
     6541 tcaaaaatta atggtgaatt tgcaggaata gatcccgagc cactctttcc ataccccaat
     6601 tttgaaggta caaacaagcg acctttatct ccttcattga attttggtaa gccgatctgc
     6661 catgccggaa tcaaactggt aaggaagaaa gagacacctg ttccgttgtc aaaaaccttt
     6721 ccattcaaaa gatagccttt ataatccacc ttgacctttg atgttgaagt tggtttcctg
     6781 gtagaagtat ccgatttggt gtctataacg taataaaggc cttcagaggt agaatcagcc
     6841 ttgatgttat attgattgag gtaagccctg attttttgct tatcaatctc caattgggca
     6901 gaatcatcta ctgatgatgg atctttttcg caagcgatca gcaataggat cggtgcgatt
     6961 aaaacaaatt tcttaagtat tttcattaaa aaaggatttt atgtctaact acaattcaaa
     7021 taaaagacga gaatagaaag gtattcgttt attgggcgga agattttctt tccaagccaa
     7081 gctttgctcc aagctccagt aaaaaatgat aagcagcatt gtattcattt tccaaatccc
     7141 cctcaagaat agcttcacgg atctcatttt tcaactcacc tacgatcggg cccgatttca
     7201 aatcgaaagt ttccatgata agttcaccgg acacaggggg ctgccaattc ctaattctgt
     7261 ctttgtcttc gatttctttt aacttaagcc tgaccatctc atagttttca agcaatcgtt
     7321 tcactttttt ctcatttttg gaggttatat ctgcctcaca aagaatcatg aggtcatcaa
     7381 tctcatcccc cgcatcgaac aatagccttc tgatggcact atccgttata tcctccttgg
     7441 ttaggctaat aggtcttaaa tgatatcgta ccagtttctg cacatatttg acatgctgat
     7501 ccaatggcat gcgaagtttt ttaaaaattt tatataccat cacagcaccc aatgcatcgt
     7561 gaccatggaa ggtccatcct tgcttttcat caaaacgctt ggtagccggc ttggcaatat
     7621 catgaaggag ggcagcccaa cgcaaccaaa tattatccga attgcgacaa acattgtcca
     7681 acacctccag cgtatgataa aagttatcct tatgcccttt gccatcctgg tattccactc
     7741 cttgcaattc aaccagctca ggcaggaaga cttcaagcaa accggtatcg aacaataact
     7801 taatgccgat cgatggctta tcacaggcca tgatcttttg gaattctgta acaattcgtt
     7861 ctatgctgac tattttcaat ctatccttat tatgattgat tgcagcatag gtgagcgggt
     7921 cgatggagaa attcaactga ttggaaaaac gaatcgctct catcattcta agcgggtcat
     7981 cagagaatgt gtcttccgga tccaaaggcg tctttatcaa cttctcttcc aggtgttcca
     8041 taccgtcaaa tggatcgagc aactcaccat aggtatcggt attgagactt atagccaaag
     8101 cattgatggt gaaatcccgc ctcagttggt cgtcgtacaa agtacctgct ataacggcag
     8161 gtttccggct gtttttctgg taagattctt tccgggctcc aacaaattca atttgaatat
     8221 cattatatct cagtgcagct gttccaaaat tcttatagac aactattggc tgcttattac
     8281 caagaagctt attgacctgc tctgccaact gaataccatc tccaacacaa actacatcaa
     8341 tatctgtaga attgcgtccc agaatcagat cccgaacata acctcctaca agataggtct
     8401 gtaggtcgag atctgaagcc gcacgggcta aaattttgta tagaaattct tcgttttcgc
     8461 ttctgtttaa cagtagaaac attgttttaa aatttaaacc gtcaactctt ctcctgcgta
     8521 atattcacgc atcttttccg taatttccac cacttcatcg aaagatgaag aatgtaccaa
     8581 cagtgtccta aactgtttta cctcgcgata tcctctgaaa taattaccat aatgacgctt
     8641 catttcattg atcccaacct tgtctccctt ccacatgatg gatctgtgga gatgctgaag
     8701 tacagcctca attcgctcgt cgagtgatgg tggatcgggt atagttccat ctttcagata
     8761 ctgttttatc tccctgaata tccagggatt acctatgctt gctcttccga tcatgatgcc
     8821 atccacgcca taacgttctt tgtacagttt ggctttctca ggagaatcaa tatctccgtt
     8881 accgaaaaca ggaatatgta tgcgtggatt ttccttcact ttggcaatta gggtccagtc
     8941 cgcctctccc gtatacatct gcttcctagt ccgtccgtga atagacaatg ccttgatgcc
     9001 tatatcctga agcctctcgg ctacttcgac aatattcttt gaatcttcat cccaacccag
     9061 tcttgtttta acggtaacgg gcaaagaggt cgcttttact actgcttcag tgagagagac
     9121 catacgatcg atatcctgaa gtatacctgc accggccatc ttgcaaacga cctttttcac
     9181 cggacatccg taattgatat caagcacctc cggttgagtc cgttcaatga tttcagcagc
     9241 ctgaatcatt gattcttttt cagatccaaa gatttgaata cccaccggtc gttcttcgtc
     9301 agaaatatcc agtttctgca cacttttatc ggcatttcga atcaaacctt ctactgaaac
     9361 gaattcagtg tatagcatat cgcatccctg ggctttgcag agtgctctga aaggaggatc
     9421 actcacatct tccattgggg caagaagcaa gggaaattca cctaatgaaa taccaccgat
     9481 tttaaccatt caatcattaa ttgaaaggac aaaaatacaa gaaataaggt aattgttggg
     9541 atcttgtctt taattcctta atacttaaca agactgaacg taatgaaaga gaaaaaggca
     9601 accacccgaa agtgattgcc ctgaataggt tgtaggcatt tttccaactc aatgcaaata
     9661 agccatagct tatttttctt cagatctacc tgaatcagta tggtcggtat gattttcagt
     9721 aacaggctca gtcataactt cctttttctt atgaaatgtc aatccggtat tgttaccttc
     9781 tgcaacaata acatcgcccg gcaaataatc tccactcaat aattgttttg acaattcatt
     9841 gaccagctct ttttgcagga cccttttcag aggtcttgct ccaaactgag ggtcataacc
     9901 cagatcaatc agcagtttag aggcacttgg ggccaattca atcctaagtt cctgagactt
     9961 aagcaatttg ttgatgcctt tcatctgaat atcgagaatc tttcctatct cttctttggt
    10021 caaaggcaag aacatgatgg tttcgtcaat cctattgaga aattcaggac gcatggtttg
    10081 tttcagcaat tcaaaaactt ccaatttcgt agtttctatt acttcagatc gccttttagg
    10141 ctcaatctcc tccaaatcct caaaattctc aagaataatg ttagaaccaa ggttggatgt
    10201 catgatgata atggtattct tgaaattgac ctcccttccc tttccatctg tcaaccgtcc
    10261 atcgtcaagt acctgcagga gaatgttgaa cacatccgca ttggcttttt ctatttcatc
    10321 cagcaatacg atggagtatg gtctatgacg cactgcctct gtcaactgac caccctcttc
    10381 ataacctaca taaccgggag gtgcgcctac caatcttgat accgagtgtt tttccatgta
    10441 ttcactcata tcaattctga ttatagcatc cttgtcatcg aacaaaacat ccgcaagcgc
    10501 tttggtcaac tcggtcttcc ctacccctgt aggtcccatg aaaataaagg atcccacagg
    10561 cctcttactg tcgctcaatc ctgcacgatt ccgtcttact gcttcactta ctgcctcaac
    10621 ggcttcagtc tgcccaatga gcattttccc tatttccttt tccatattga gcaatttgtc
    10681 tttctcgctt tgctgtaatt tggagacagg tatgccggtc catttagata cgacatccgc
    10741 gatatcgtta gcagtaacca tttgagcggt gaatcgtgaa ccttcaggca gtgcattcag
    10801 ctcgttttct gactccttca attcctgttg tttggcctgt agttctccat aacgaatctt
    10861 ggcaacagtc tcaaagtcac tctctcgttc agccttctgc gcctttcggt ccaactcttc
    10921 gatctcttcc cgcaatgtat tgatacggtc aacaatggct ttttcatttt tccatttggc
    10981 catgacagaa tccagttgtt gtttggcgtt ggcgatggat tcagaaatag ccgccagttt
    11041 cttgtcatct ttttctcgtt tgatggcttc acgttctatc tcaagctggc gtaccttcct
    11101 gtccagttca tcgattgaat cgggcatcga gttgagctcc agtcttagct tggcagctgc
    11161 ttcatcgatc aggtcgatcg ccttatccgg caattgtcga tctgcaatgt agcgatgaga
    11221 catttccgcc gctgcaatca acgcttcatc cagaatttca atcttatgga agacttcata
    11281 tttttcctgc agtcctcgaa gaatggagat ggtatcttcc acactaggct catctaccat
    11341 cacattctga aaccttcgta ccaaagcagc atctttttca aaatacttct gatactcatc
    11401 caaagttgta gcaccaatgg catggagctc acctcttgcc aatgcaggct taagaatatt
    11461 ggcagcatcc attgccccac tgcccccgcc tgcaccaatc aaggtgtgga tctcatcaat
    11521 gaaaagtatg atttctccat cgctttctgt aacctccttg atgacggctt ttagcctttc
    11581 ctcaaattca cccttatact ttgcaccggc aactaatgat gataggtcca gactgaatat
    11641 agtcttactc ttcaggtttt caggcacatc ttgcttaacg atccgccagg ctataccttc
    11701 aacaattgca gtctttccta cccctgcttc acctaccagg atagggttgt tcttctttcg
    11761 acgtgaaagt atgtgcataa cgcgtctaat ctcttcatcc ctcccgatga tagggtcgag
    11821 cttgcccgat tcagcccttt cattcagatt aattgcgtat ttaccaagtg aattatagga
    11881 actgtctccg ctctgagagt taaccttatt gccctttctc aattctttga tggcagagac
    11941 cagtcctgct tctgtcagcc cgcgatcttt taaaagtctt gcagtgcggt cgcttccctt
    12001 gaatagaccc aggaaaagca attccaaaga aatgaattcg tcgccaaact ccttcattgc
    12061 ctccttcgct tttgagatcg cattattggc atcattggaa agaaattgct tgtccgagcc
    12121 ctgcactttc ggaattaaat caatcaattt gtcattttca tctttaatgg gattaagatc
    12181 cactgccatt ttattcaata aaaatgaaac tacactttcg tcagtgtcaa tgatcccacg
    12241 aagaatatga gcggtatcaa ccgtttgctg agaacggtcg gatgcaattt tttgcgcctt
    12301 gatgatagca tcctgcgcat ttattgtgaa attatccagt gtcatagttt tcagttttac
    12361 ttgacttgta tcaatcaaaa aacaagccaa tataagaact attctactta tatgaaaaaa
    12421 tggcaggcgc ccaacgaaga acatgcaatt atgtcactga taaccatatg tttaggacaa
    12481 aatgacaact gtgcaactgc attacggaat gctataccaa gatgatttac ataatagtcc
    12541 ataaattttt ggtatacttg tccggatagc tatgctacta gagctctgct ctgtcccggg
    12601 agtgaaacaa tccggggttc cgggatgccg aggttacagt cgtatagatc aatgaaattg
    12661 gtcgatagcg aatgtacctt cggtattata tttaggattg accaaagaga aacatttcca
    12721 gattctttca atctaaatac tttatgaaac cgggcaataa tttataattc tcctgtgtat
    12781 tatttagatt ggctgattga atttgcacta tgaagagttt ccacgggact tcatgcggca
    12841 ataacaggag aatttttcct tgaataacga aaaaacattc ataacaaatg caaaaatatc
    12901 aaggccagca ttgccggtat tgcctgcacg agaaaaattt tccgtgaaac tgaataaccg
    12961 ccaaatatcc ctgcaacgac tacacatcca agaaaaaacg tcataacatt aatctgccaa
    13021 agggaatccg atatcagaaa actccagatt agaccggctg ctaaaaaccc gttatataac
    13081 ccctgatttg ccgctatgct ttttgtctgt ctgaaaaaat caccctgcct atttcctttg
    13141 aagaatttcc gcccaactgt ttcccacgca aacatttcaa gccacatgaa aaataaatgc
    13201 tggattgcta ctattgcaac aagtgttttc gctatcatga tgccaatgct catacttaaa
    13261 aaatttaggt ttttcacgtt atcctgatgg tgcaactcac ggatttcgag taactatcca
    13321 caagcaatgc caactgttat ctcaaaagta tcaactagga gcagacactg caatccctgg
    13381 gcttctgtcg gttcaactat gctaatatga taccggtcag gtcgccaaag ttgaaacaag
    13441 atacacaatg tttatggtat acaatcgatt aaattaatta tctgcataaa tgtacttttg
    13501 agagcgccca agtagtcttt aacactttac agaattaatt ttcggacact caaattttaa
    13561 taataagata tattaatata atatataata cacttactat ctatatatta taacacaaac
    13621 acatagcttt tatcatttat tcaccccgta atacttttca atagaagcaa agaagaattt
    13681 gtgatactca tctctcaccc cttgatgctg tccaattatt ttttgcatct ttgcagcaca
    13741 atggcagatc agaatttcat cgactatgtt aagatatgtt gccgttccgg taaaggcgga
    13801 gcgggttcgc atcactactt tagggataaa atgaactcaa aaggtggtcc ggatggcgga
    13861 gatggaggtc gaggcggaca tattattctg aaagggaata gaaacgtatg gacattgatc
    13921 aaccttaaat accgaaagca tgtcattgcc gaggacggtg tcaacggatc taaaaacaac
    13981 cgcaccggcc gcagcgggaa ggatgtcatt ttagaagttc ctct
//
LOCUS       DAOQQN010000023        13992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1449_length_13992_cov_5.600918, whole genome shotgun sequence.
ACCESSION   DAOQQN010000023 DAOQQN010000000
VERSION     DAOQQN010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13992
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1449_length_13992_cov_5.600918"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PLG24_01575"
     CDS             <1..268
                     /locus_tag="PLG24_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23599.1"
                     /translation="SWQYHFRRAEVWRCIHGDITVITSDTDEEKYSRILESGDIIRLA
                     QGERHRIIGNDDYGIIAEIWQHTDASNPSNEEDIVRLQDDFGRK"
     gene            462..848
                     /gene="apaG"
                     /locus_tag="PLG24_01580"
     CDS             462..848
                     /gene="apaG"
                     /locus_tag="PLG24_01580"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HMZ23600.1"
                     /translation="MTSLTTKGVNVDIKVDFHTESDTDGRAGFIFIYHISIRNHNRFP
                     VQLLSRHWRIFDSNGEVRYVDGEGVVGLQPIIEPDQIFDYSSFCNLSTELGTMSGTYT
                     LENLHSGNLFEVDIPTFELATPAIMN"
     gene            complement(1100..2758)
                     /locus_tag="PLG24_01585"
     CDS             complement(1100..2758)
                     /locus_tag="PLG24_01585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ23601.1"
                     /translation="MKRTKGNLIRSLMAGLNKAVHEYQLETSSKEGVARYNRRTFIQQ
                     STKGAIGVGVALSLPAMLISCKQETRPPQGVEQDPVLDVAILGGGMAGLNCANHLLGS
                     GLTFKVYEGSKRVGGRILTHYNDSMQLGIFPEFGGDFIDSDHEDMIKLAKEFNLDLVD
                     QIAEQEANQWVKDIYFFDNRKISEKEIIREFKKIAPKIAKDRDALGENYDTPEASRMD
                     KMSLSEYLDSLKCKEWMKELLDAAFVAEFGLDCSEQSAINMLDLIITDTSEGFKVFGT
                     SDERYRIKGGNSQITNKLAEKIGEDNILKNYKVTEISEGDNGMYTIVFENGEKVEAKV
                     IVCTIPFTILREIKLSLKGMTPEKRKCIDELGYGINTKLVLGYEGQPWRSKENNAMGY
                     LFTKDMTNGWDASLNRTENNENGAYVAFFGGKFSQSLCDESSRNPMSPPEHVWRTMLP
                     EKRVNGFVDDLDKIFIRSKKKFKGKHVFVNWIEYPFVKASYACYKTGQWTTIAGHEAK
                     PVGNFYFAGEHCSNNFQGFMNGAAESGRETAIQLIEKYAKVPVN"
     gene            complement(2920..4365)
                     /locus_tag="PLG24_01590"
     CDS             complement(2920..4365)
                     /locus_tag="PLG24_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ23602.1"
                     /translation="MFRIIPIALFCLLAYISNGQTASEDKINTANKALAFLNKIADMN
                     KSGSRAFTLKFDTLSIFFAGPEEPESLVSTMPVTYDAWGRIKDARIIEAGFEDYYQYR
                     YNYTGDDNRFSHLDYYLHSGGEEHYAGVQIHLYDDHGRLISSRELDKDNQLIYGDSLS
                     LEFNGLGQIVFYEYLYFQLGRWNSGGKVYDVQYSGQDIVGYNADEAERGHSGKDTIVS
                     YRYEDVRFLNDDSSILFPDFDEFFEIDSLGVDNFFLSSRDAAKAYVKQPVQYLKYRTD
                     GSGALVQSAVYDPDKFISIISDPNGKEIYRIEYSFDGQGRLKGIHKVETGQVPEVSTF
                     EYNELGRMARYIFTSDSVNFRVNNLYVTDDKDRLSEYYQNLEDTNNSITNILRFGYRG
                     ENKTYTGSILEACVFPNPAGHAIYIKTPQSIYQEMQVSMMGNDGRLVFSGRINFTAGV
                     GSIPVAKVPPGTYLLLIKDGKKAYKATVVII"
     gene            complement(4528..5076)
                     /locus_tag="PLG24_01595"
     CDS             complement(4528..5076)
                     /locus_tag="PLG24_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HMZ23603.1"
                     /translation="MHRISAWILKMLGWKIVGWDMNALKKYVLIAMPHTSNLDFPLGL
                     LVRSSIKLKIHFVGKSGLFRWPFGFIFRRLGGYPVDRSRSHNYVEAVAAMFKDKDLFR
                     LTIAPEGTRSKVDRLKTGFYYIAQTARVPIVMVAFDYKTHEIRVHPPIDSSISYDDLY
                     QKMKEFFRGVEGRHPEKDYEFE"
     gene            complement(5635..6447)
                     /locus_tag="PLG24_01600"
     CDS             complement(5635..6447)
                     /locus_tag="PLG24_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ23604.1"
                     /translation="MHFYEKTVLITGGCSGIGKIMARKSLERGASRVIIIDVNDAVFQ
                     DLKKEWEAFSGEVITFHADLSKVDQIRDVAARVKEAYGVPDVLINNAGIVTGKYFYEH
                     SHENISLSMAVNADAMMHLTLEFLPGMIERNSGSIGNLCSLAGLIGNTKMAVYVASKW
                     AAVGWSESLRLEMQQLGKDIKVTTIMPYYINTGMFDGVRSGLIPILKPEPSSEKFIRA
                     IERERKLFATPVPYWFIRLSQGILPVSAFDFVMKNIFGVYDTMKKFKGRDGN"
     gene            6728..7654
                     /locus_tag="PLG24_01605"
     CDS             6728..7654
                     /locus_tag="PLG24_01605"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HMZ23605.1"
                     /translation="MTYFTHTILIISIFEKSIVNPMKNLFLSLLLIFPALVQAQELKP
                     YQFYNSRGKAVTFGKMINSLKDYDVVLFGEYHNNSVNHWLELKTAEALFEKKGNRLIL
                     GCEMYERDNQEAIDRYLSGDINDEKLGEEARLWNNHKTDYLPILEFARQKKLLMIATN
                     VPRRFAALVAKKGKDSLKLESHEVPWVVKLPYEVSMETPGYDEMLKMMGDHAGSRAMN
                     FVAAQAIKDATMAESILNHLSPGTLFFHYEGDFHSKEHGGIYWYLKKARPELKIAVIS
                     ISESADPALKLPSDFKKTEFNLVMPQDMTKTY"
     gene            7676..8287
                     /locus_tag="PLG24_01610"
     CDS             7676..8287
                     /locus_tag="PLG24_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23606.1"
                     /translation="MNNTIQRMFDKEEIIRQVAQVLKETRTAQDTVRQGPGRDRRFTL
                     LARYMIEKAIRHNALLTDDTGTGIALMFRMNAGRNHFFRDVWEDIKLAFCVIGLTNVP
                     KIIRVRKHIRSRQPKSEPFFYMWFWGVIPGSRGAGKGVAVNMKDMFLRISEDEQLPIY
                     AETRLRTNAMVYQRYKFIHYDTWNMPGGDTMWFVRYLPKSLGR"
     gene            8873..9409
                     /locus_tag="PLG24_01615"
     CDS             8873..9409
                     /locus_tag="PLG24_01615"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HMZ23607.1"
                     /translation="MSKTNNFVFWALYSVAWIIFVGLSIEAGGLLVNFIISLYKPELI
                     QSLYQKLDLTEIYKESRLAFFCYYSFILTIAILKACLFYTVIRLMHTMDMSKPFSDFV
                     AKQIALISYYTLVIGFLSYLAHQVTKTMMHHGLVTDKLRPFWEDSDAYILMGGVVYII
                     GVIFRKGVAIQNENDLTV"
     gene            9413..9628
                     /locus_tag="PLG24_01620"
     CDS             9413..9628
                     /locus_tag="PLG24_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ23608.1"
                     /translation="MAIIVNLDVMMAKRKMSLNELSEKVGLTLSNLSILKTGKAKAIR
                     FSTLEAICKVLECQPGDILEFVEEDRD"
     gene            9795..10880
                     /locus_tag="PLG24_01625"
     CDS             9795..10880
                     /locus_tag="PLG24_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ23609.1"
                     /translation="MKKKKNSILRQIAKYILRGIGIIILIFLTWSMIPIRQTIRPIQK
                     RAETKYWTMRDGSKIAYSLLKGDSANINPPIIYLHGGPGGYVHSAIIKQMDEVAKKGY
                     DVYLYDQIGSGLSDRLSRPKDYSFERHLNDLKEIINSHIMSDKVILLGHSFGGILAVH
                     FAARFPNSIDRLILSSPGDLQPYRVNADGTMADMGRLYPTPANYQFKAPIEVFEQTEK
                     DFLQPRIVMSMFCALAFNIKWASDKEFDDYTNTMASKFTLGMVADPRNVKMEEGGAGG
                     YSHGFSNYYGDLKDAREKLRNLNIPTLVMQGQYDQGEYSSVYEYVDLLKGNYIFIEDA
                     GHIIWWDKPNEFNRSVIEFLMKKSSVQ"
     gene            10954..11310
                     /locus_tag="PLG24_01630"
     CDS             10954..11310
                     /locus_tag="PLG24_01630"
                     /inference="COORDINATES: protein motif:HMM:NF019473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HMZ23610.1"
                     /translation="MSAQKKIVEKYMEGFRRTDHKEILSCLTEDVVWEMPGFYLHRGI
                     EAFDKEIENPNADGHPDIKVIRLIEEGNIVVAEGSVKATLKDGKKLDAVFCDVFHFRG
                     GKISKLTSYLMFYNLP"
     gene            11370..11771
                     /locus_tag="PLG24_01635"
     CDS             11370..11771
                     /locus_tag="PLG24_01635"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HMZ23611.1"
                     /translation="MITIKALIKADVQKVWDYYTLPHHIIRWNFAGPGWHCPKAENDM
                     RPGGTYKARMEAKDGSFGFDFEAIYTDVQPGKEFSYEFGGRKASVSFSKNGEETEIVI
                     CFDPETTHSIELQRSGWQSILNNFKSYTENN"
     gene            complement(12427..12570)
                     /locus_tag="PLG24_01640"
     CDS             complement(12427..12570)
                     /locus_tag="PLG24_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23612.1"
                     /translation="MMAFHGGAHYNDNIKKNYGKNGKIGYSITFITGSLSGCTSISSP
                     YRQ"
     gene            12630..13415
                     /locus_tag="PLG24_01645"
     CDS             12630..13415
                     /locus_tag="PLG24_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23613.1"
                     /translation="MSSKYITLIMIYLATGIIYLTAGLSKFSPGNIGNLIGPVDYRKI
                     SDSTVFLLFMQGIAVLQVVVGALLLSQRYSVFGLIMVLPMSLGILIFTIVAGFGLTPI
                     INLLLLLLLLYAFYLEKEAIRQLFRKDRKGILFKSPSFQQFPERLVPNIALILIGLTV
                     LVSFLDNIVLNILTTTALLLFTINLFQRKDYIFADKILIFLFLMISSITVNGMLINKM
                     IAKGFYVVFLLIPMGILIYLFRLVYWKFSEKNVQDRQNQVKEG"
     gene            13613..>13992
                     /locus_tag="PLG24_01650"
     CDS             13613..>13992
                     /locus_tag="PLG24_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23614.1"
                     /translation="MIRFLIAIILTTSMNVVTPAQIETISTKDTTTYSEKQAMAEDRF
                     VQATSLRLIGKSKEAIPILQELIKQDKSEASYYYELARSLFVEKDYDNALMNANKAVS
                     LDKGNLFYALFEAQIYEKQSNPAIA"
BASE COUNT         4212 a         3006 c         2717 g         4057 t
ORIGIN      
        1 cagctggcag tatcattttc ggagagcaga ggtttggcgg tgcattcatg gggatatcac
       61 cgtaatcaca agcgatacgg atgaagaaaa atattcccga atcctggaaa gcggcgatat
      121 cattcgcctt gcccagggcg aaaggcatcg tatcatcggc aatgatgatt acggaatcat
      181 tgcagaaata tggcagcata ccgacgcatc caatccaagt aatgaagagg atatcgtaag
      241 gttgcaagat gacttcggcc gtaagtgaca gcaaaaaaat tttctgtgag gaaggtccct
      301 gatgatgtac tcttcaatcc ataccatgaa aatatacaag tcttttgtca ggactttttg
      361 ttaaatcatg acttttagga atggtttttg cagtgaaact atagccggaa ctaaacagat
      421 ccggaaatag gtattcaaag aatgtcaaat acagcaacaa catgaccagt cttactacta
      481 aaggagtgaa tgtcgatatc aaagtggatt ttcatacaga gtcggatact gacggacggg
      541 caggattcat ttttatttac catatttcca tccgtaatca taaccgcttt ccggtccagt
      601 tgctcagcag gcattggcgc atctttgata gcaatggaga agtgcgatat gtggatggcg
      661 aaggcgtggt aggactgcaa cccatcatcg aacccgatca gatcttcgac tactcaagct
      721 tctgcaattt aagtaccgaa ctgggtacta tgtctgggac ctacactttg gaaaatctgc
      781 attcaggtaa tctttttgaa gtggatattc cgacttttga acttgcgact cctgccatca
      841 tgaattaaat accacagaat cattccgttc aaaactatat cttttcagac cggtaacctt
      901 atccgtcata gccaatattt ctttcattaa caccggatac tttccgactt cctggcatca
      961 ttaaaggctt aatgaataaa agcatgcgga ctttatctcc tgggtaatat taatttcaat
     1021 ttgtattccg atatcagtag aggtgaataa ttacaatgtc accaacaaac aaccacattg
     1081 cctgatagct gctcatatct tagtttacag gaactttagc atatttttca atgagctgaa
     1141 ttgctgtttc cctgccactc tcagcagctc cattcataaa tccctgaaaa ttgttactgc
     1201 aatgctcacc ggcaaaataa aagttaccga ccggttttgc ctcatgaccg gcaatcgttg
     1261 tccattgccc ggttttataa caggcataac tcgcctttac aaacggatat tcaatccagt
     1321 tgacaaatac atgctttcct ttaaatttct ttttcgagcg aatgaaaatc ttgtcaaggt
     1381 catctacaaa cccattgacc ctcttttctg gtagcattgt acgccaaaca tgttccggtg
     1441 gggacatagg atttcgcgag gattcatcac aaagcgactg cgaaaacttg ccaccgaaaa
     1501 aagccacata tgcaccattc tcgttattct ccgtgcggtt cagactagca tcccatccat
     1561 tggtcatatc tttggtaaac aggtacccca ttgcattatt ttccttactc cgccatggct
     1621 gaccttcata acccagcacc aatttggtat tgataccgta acccagctca tcgatgcatt
     1681 tacgtttttc aggtgtcatt ccctttagtg agagttttat ttcccgaaga attgtaaagg
     1741 ggattgtgca tacaattact tttgcttcga ccttctctcc attttcaaag acgatggtat
     1801 acattccgtt atctccttct gatatctccg ttaccttata gtttttgaga atattgtcct
     1861 caccgatctt ctctgccagc ttgtttgtga tctgcgaatt ccctccttta atccgataac
     1921 gctcatcact ggttccaaac actttaaatc cttcagatgt gtctgttatg atgagatcca
     1981 gcatattgat ggcagattgt tcactacaat ccaatccaaa ttcggctaca aaggctgcat
     2041 ccaataactc tttcatccat tctttgcatt tcagtgaatc gagatattca gacaacgaca
     2101 tcttatccat tcgacttgct tcaggcgtgt cataattttc gccaagcgca tccctatcct
     2161 ttgcaatttt cggtgctatt ttcttaaatt cacggatgat ttccttttca gaaatttttc
     2221 tattgtcaaa aaaataaata tcctttaccc attgattagc ctcctgttca gctatctgat
     2281 ccaccaggtc cagattaaac tccttcgcaa gtttgatcat atcctcatgg tcggaatcaa
     2341 taaagtcacc tccaaattca ggaaatattc ccaattgcat agagtcgtta taatgtgtga
     2401 ggattcgccc gccgaccctc ttacttcctt catacacctt gaacgtaagc cctgatccca
     2461 gtagatgatt ggcacagttt aatcccgcca tccctcctcc aaggattgca acatcgagga
     2521 cagggtcctg ctctactcct tggggaggcc gtgtctcctg tttgcatgaa atcagcattg
     2581 ccggcagact caaagcgaca ccaacgccta ttgctccttt tgtggactgt tggataaaag
     2641 tcctccggtt gtatcttgca acaccttctt ttgatgaggt ctccagttga tattcatgca
     2701 cagctttatt caggccggcc ataagtgacc tgatcagatt ccctttggtt cgtttcataa
     2761 aagtggtgtg aaggctcccc ttccggtttt atttgacaga aaatattgtt ccaatatatc
     2821 caactcctcg caaaccaatg ctacacaaac tatccgaaac ttcccggata atgcctgcaa
     2881 tttatgaaat tataaataaa aatagtgcga gtcccctcgt taaattatta ccaccgtagc
     2941 cttgtaggct ttttttccat cctttatcaa aagtaaatat gtgcccggag gcaccttagc
     3001 cacggggatc gagcctaccc cggctgtgaa atttattctt ccggaaaata ccaatctacc
     3061 atcatttccc atcatactaa cctgcatctc ctgataaatc gattgcggag ttttaatata
     3121 aatggcatgc ccggcaggat tcggaaatac gcacgcctcc aaaatacttc ctgtatatgt
     3181 tttattctca cccctataac caaaccttag tatattggta atggaattgt tggtatcttc
     3241 cagattttga tagtattcac tgagtcgatc tttatcatct gtcacataga gattattaac
     3301 cctgaaatta acagaatccg atgtgaaaat atagcgagcc atacgcccca attcattata
     3361 ttcaaaggtc gatacttccg gtacttgtcc ggtttctacc ttgtgaatcc cctttagcct
     3421 tccctgacca tcaaaagaat attcaatcct gtatatctcc tttccgttcg gatcactgat
     3481 gatggaaata aatttatccg gatcatacac tgcagactga accaaggctc ctgaaccatc
     3541 tgtcctgtat ttaaggtatt gcaccggttg tttgacatat gccttggcgg catccctgct
     3601 gctcaggaag aaattatcaa cgcctaacga gtcaatttca aaaaattcat caaaatcagg
     3661 aaaaagaatg gatgagtcat catttaaaaa cctgacatcc tcgtatcggt acgagacaat
     3721 cgtatccttc ccggaatgcc ccctttccgc ttcatcggca ttatatccta caatatcttg
     3781 tccactatat tggacatcat aaactttccc accagagttc catctaccca gttgaaaata
     3841 aagatattca tagaatacga tttgcccgag tccattgaat tctaagctca acgaatcacc
     3901 atagatcaat tgattgtcct tgtccagttc cctggaagaa atcaatcgtc catgatcatc
     3961 ataaagatga atctgaacac cggcataatg ctcctcacca cccgaatgca gataatagtc
     4021 cagatgtgaa aatctgttgt catcaccggt ataattgtat ctgtattggt aataatcttc
     4081 aaagcccgct tcaataatcc ttgcatcctt gattcttccc catgcatcat aagtcaccgg
     4141 catggtggac actaaggatt ccggctcttc aggccctgca aaaaaaatgg acaacgtatc
     4201 aaacttcaag gtaaaagcgc ggctcccgga cttattcata tcggcaatct tattcaggaa
     4261 tgcaagagcc ttatttgcag tattgatctt gtcttcagaa gctgtttggc cattggaaat
     4321 gtaggccaat aggcaaaata gtgcaatcgg gattattctg aacatcgatt tttggttaat
     4381 atgaaaggtc gaatgtacgg agaataaacc ggctaccaga aaatttaaca catgatgtat
     4441 ccggatcttt ttcctaccaa tataacgctc caagctacca tgccggcctc aaggattgat
     4501 ttcagcaaat catcatcaaa aggatttcta ctcaaactca taatccttct ccggatgtcg
     4561 accttccacc ccccgaaaga attctttcat cttctgatat aaatcatcat aggaaatact
     4621 ggagtcaatt ggtggatgaa cccttatttc atgtgtctta tagtcaaatg caaccatgac
     4681 aatcggtaca cgggcagttt gtgcaatata ataaaaacca gtcttcaacc ggtccacctt
     4741 gcttcgggta ccttcaggcg caatggtgag tctgaacaag tctttgtcct tgaacatggc
     4801 ggctactgct tccacataat tgtgacttct gctgcgatcc accggatatc cccccagacg
     4861 tcggaatatg aaaccaaatg gccagcggaa tagaccggac ttacctacaa aatgtatctt
     4921 gagctttatg gaactgcgca ccagcaaacc aagagggaag tcaagattag aagtatgggg
     4981 catggcaatc aggacatact ttttcaaagc attcatatcc cagcctacaa tcttccaacc
     5041 caacattttc aaaatccaag cgctgatcct gtgcatctct gttttttcaa tcaaatataa
     5101 cgtttatgtc tgtatatcca atgatggatg tttatcaacg gtgcacctca tatatttttc
     5161 taagatttga tgagcagtct caataacttt tcaaaccaat tttcaacatt catacctgtt
     5221 atttccggcc cattcaattc gactgtctaa ggagtgatta ttaggttgac tcctgtacct
     5281 acattgttct tgcctctttc ccctttacta aaaactatag ggcgcaacaa gccctcccgc
     5341 ttctgtactt tgtcttgaat cggatttttt cggctgaata ttccggttat acctgtgccc
     5401 atggacaaac acccggctca aaagttttat aaactcgtaa caatacacag atcgaaattt
     5461 taggttaatc tatgtccctt atcaggtatg gacaaaagtt gtcgcatata agggatgaaa
     5521 gttagcccgg gagcaacaag cataattcca tggtcttacc ttcaaaaagt cggactattc
     5581 gtaaggttat taataatccg acacctttta ttcactggag ccaataaaca aaattcaatt
     5641 tccgtctcta cctttgaatt tcttcatggt gtcataaaca ccgaagatat tcttcatcac
     5701 aaaatcaaat gctgaaaccg gcaatatacc ctgactcagg cgaataaacc agtacggcac
     5761 cggcgtagcg aacaactttc tttccctttc aatggcccgt ataaattttt cggacgatgg
     5821 ttctggtttc agaatgggaa tcaggccgga ccggacaccg tcaaacatgc cggtattgat
     5881 ataataaggc attatggttg tcaccttgat atctttgcca agctgttgca tttcaagtct
     5941 gagactctca ctccagccca cggccgccca ttttgatgca acataaaccg ccattttcgt
     6001 attgccgatc aagccggcca gggaacataa attgcctata gaccctgaat tacgctctat
     6061 catgcccgga agaaattcga gtgtcaggtg catcatagca tcggcattga ctgccatgga
     6121 aagtgaaata ttttcatgac tatgttcgta aaaatattta ccggtgacta tgccggcatt
     6181 gtttatcagt acatcaggca ctccataagc ttctttgact cttgcagcca catctctgat
     6241 ttggtccact ttggaaaggt ccgcatggaa ggtgataacc tcaccggaaa aggcttccca
     6301 ttctttcttc aaatcctgaa ataccgcatc attcacatca atgataatga cacgtgatgc
     6361 gcctctttca agggatttgc gtgccataat cttaccgata ccggagcagc cacccgttat
     6421 taaaaccgtc ttttcataaa aatgcatatc cctccatcaa tttaaaacat aaagttaatc
     6481 gaaatatcgg acttgggatg atgctgtaaa agattacata cctaccaatt tatctgaccg
     6541 gattacagtt gggcttaaat gtatggctga ctctagaggt cacctttttc tttggagcgg
     6601 aatccgaaaa ggcattgtgt cgcaggacaa gccgcaatta tatcctcagc tgtctcaaca
     6661 aatcaaatca ggaattttga aatcaaacag gaaatcaact tcctgttcga cttcaaaacc
     6721 aattagaatg acatatttta cccacactat ccttattatt tctatttttg aaaaaagtat
     6781 cgtcaatcca atgaaaaatc ttttcctgtc tctgctgttg atttttccgg cattggtgca
     6841 agcccaggag ctgaaaccat accaattcta taattcccgt ggaaaggccg tcacttttgg
     6901 taagatgatc aacagcctga aggattacga tgtcgtactc tttggcgagt atcacaacaa
     6961 ttcagtcaat cattggctgg agttgaaaac agcagaagca ctctttgaaa agaaaggaaa
     7021 ccggctcatt ctgggttgtg agatgtacga acgcgacaat caggaggcga tagaccgata
     7081 tctcagtggt gacatcaacg atgaaaagct gggtgaagaa gcccgacttt ggaataatca
     7141 taagacggat tacttgccaa tcctcgaatt tgccaggcaa aagaagcttc tgatgatagc
     7201 caccaatgta ccgcgtcgtt ttgcagccct ggtcgcaaaa aaaggcaagg acagcctgaa
     7261 gttggagtca catgaagtgc cttgggtggt caagctgccc tatgaagtca gtatggaaac
     7321 acccggctac gacgagatgc tgaaaatgat gggtgaccat gccggcagcc gcgccatgaa
     7381 ttttgtcgct gcacaggcca tcaaggatgc gacgatggct gaatctatcc taaaccatct
     7441 ttcaccgggg accctcttct tccactatga aggtgacttt catagcaagg aacatggcgg
     7501 tatatactgg tacctcaaga aggcaagacc cgaactgaag attgccgtaa tctccatatc
     7561 ggagtcggca gatcctgctc tcaagctgcc ctcagacttc aaaaaaacag aattcaatct
     7621 ggtaatgcca caggatatga cgaagactta ttagactgaa aattcttgtg tatttttgaa
     7681 taatacgatc caacgaatgt ttgacaagga agaaatcatc agacaggtag cccaagtgct
     7741 gaaggaaaca cgcaccgcgc aggatacggt ccggcaggga cctggcaggg acaggcgatt
     7801 taccttactt gcgcggtata tgatcgaaaa ggcaatccgc cacaatgcct tacttacaga
     7861 tgatacagga accggtatcg cattgatgtt tagaatgaat gccggtcgga atcacttctt
     7921 ccgggatgta tgggaagata tcaagctggc tttctgtgtc attgggctta ccaatgtccc
     7981 gaaaatcatc cgggtacgga aacacatccg cagccgacaa cccaaaagtg aaccattctt
     8041 ctatatgtgg ttttggggtg tgatccccgg cagcagggga gccggcaagg gagtcgcagt
     8101 caacatgaaa gatatgtttc tacgaataag tgaggatgag caactcccca tctacgctga
     8161 aacccgactc cgtaccaatg ccatggttta tcagcgctat aaattcatcc attatgacac
     8221 ctggaatatg ccgggtggag atacaatgtg gtttgttcgg tatttgccaa aaagtttggg
     8281 taggtaaatg aaaaaaatat tgactaaatt acaacatgag aatcaaaaca ccgaaaacag
     8341 ggacattgtc cggaacactt acgtcctgaa attttcagga gatggatttg aacagaatcg
     8401 tataaggctg attaaagaag gacggtagaa atgcttcaat tttaccgcta ttttcccata
     8461 tacttgaatc ttccctccaa agcaatcagt agcattaacc catcagaaat gcttaagtgt
     8521 acacgacatg ccgaacctgg aaggcatgga cacctacaca atgctgttgt ccggcttttt
     8581 accatttaaa aatgtcgtag aataagtaaa atcttatctt ataccaagat gatttatata
     8641 atagtccata aattttaggt attaaaagga ttatatggat cgaccctcaa tccataatac
     8701 tatccatctt gtgttttctt ttggttcact tgatggcaac cgtgcaaata gctcccgtcg
     8761 atcgacaccc catctggaat aaagataaaa ttttattgtt ttttatttta tttttattgt
     8821 ttatcaataa ttatttatta tctttgcatc atcaatcaaa ataaaattac caatgtcaaa
     8881 aacaaacaac ttcgtattct gggcccttta ttcagtagcc tggatcatat ttgtaggcct
     8941 gtccatcgaa gcaggaggct tattagtcaa ttttatcatc agtctttata agccagaatt
     9001 gattcaaagc ctataccaaa agctggactt aaccgaaata tacaaagaga gcagattggc
     9061 cttcttctgt tactatagct ttatcctgac cattgcaata ttaaaagcgt gtttatttta
     9121 tactgtgatc agattgatgc atactatgga tatgtccaag ccattcagcg actttgttgc
     9181 aaagcaaatt gccttaatca gttattacac ccttgttata ggatttttaa gttatttagc
     9241 tcaccaggtg acaaaaacca tgatgcatca tgggcttgtg actgacaaat tacgcccgtt
     9301 ttgggaggat agtgatgcat atatattaat ggggggagtg gtttatatca ttggagtgat
     9361 tttcagaaaa ggggtggcta ttcaaaacga aaatgattta acagtataag atatggcgat
     9421 tattgtaaac ctggatgtaa tgatggctaa gcggaaaatg tcgctgaatg aactgtcgga
     9481 aaaagttgga ttgacacttt cgaatctttc catattgaaa accggaaaag ccaaagccat
     9541 acgtttcagc acactggaag ccatttgtaa agtattggaa tgtcaacccg gggatatttt
     9601 ggaatttgtg gaagaagacc gagattgaat caataacaca aggctgtttg tcctaaactt
     9661 gtataatgtc ggtatggata acagcaaata cacggttaag tataaggttg taccatagaa
     9721 agtggattgt ccggataaaa gagaaagttt gaaaccaaaa atattagctg gccaaataag
     9781 tattcaaaca gttgatgaaa aagaagaaaa attctattct cagacaaatt gcgaaatata
     9841 ttttacgagg tatcggaata atcattctga tatttctcac ctggagtatg attccaatcc
     9901 gccaaaccat tcgtcctatt caaaagcggg ctgaaacgaa atattggaca atgcgggatg
     9961 gctccaaaat tgcttattcc ttgttaaagg gtgactctgc caatatcaac cctccaataa
    10021 tatatttaca tggcggaccc ggtggttatg ttcattcagc aatcattaaa cagatggatg
    10081 aagtcgccaa aaaaggctat gatgtctatc tgtatgacca aattggctcc ggattatcag
    10141 accgactctc ccgaccaaag gattattcct ttgaaaggca tttgaatgac ctgaaagaaa
    10201 tcatcaattc acacatcatg tctgacaaag ttatattgtt aggacattct tttggaggta
    10261 ttctggctgt tcactttgct gcccgttttc cgaatagtat tgatagactg atactttcgt
    10321 caccgggaga cttgcagcca tatagggtaa acgctgatgg cacaatggca gatatgggta
    10381 gactttaccc aactcctgcc aattatcaat ttaaagcacc tattgaagta tttgaacaga
    10441 ctgaaaagga ttttcttcag ccgcgaatcg tgatgtcgat gttttgtgcc ttggctttta
    10501 atatcaaatg ggcaagtgat aaagagtttg atgattatac taatacgatg gcatctaaat
    10561 ttaccctggg aatggtagcc gacccaagga atgtgaaaat ggaagaaggc ggggcgggtg
    10621 gatattcgca cggattcagc aattattacg gagatctgaa agatgcaaga gaaaaacttc
    10681 gaaatctgaa catcccgacc ctcgttatgc aagggcaata tgaccagggg gaatattcct
    10741 ccgtatatga atatgtggac ttgctcaaag gcaactatat atttatcgaa gatgcgggac
    10801 acatcatatg gtgggataag cccaatgaat tcaaccggtc tgtcattgag tttctgatga
    10861 aaaaaagtag cgttcaataa acaataattt gatccgaatc attccctatt gctaacttta
    10921 actaatttaa aatcttaagg tagccaagta aatatgagcg ctcagaaaaa aatagttgaa
    10981 aagtatatgg aaggattccg aaggaccgac cataaagaaa tattgtcatg cctgacagaa
    11041 gatgtcgttt gggaaatgcc gggtttttat cttcatagag ggattgaggc ttttgacaag
    11101 gaaatcgaaa atcctaatgc tgacggacac ccggatataa aggtaatccg gttgattgaa
    11161 gaaggaaata tagtcgtcgc agaaggaagt gtaaaagcaa ctttgaaaga cggaaagaaa
    11221 ttagatgccg tgttttgtga tgtttttcat tttagaggag gaaaaataag caagctaacg
    11281 tcttacctaa tgttttacaa tctaccgtaa cttacatttt aagttaatac caatgggtta
    11341 aaattgtgaa ataaaaaata gagaacatca tgattaccat aaaggctctt ataaaggcag
    11401 atgtacaaaa agtgtgggat tactatacac ttccacatca cattattcga tggaattttg
    11461 caggcccagg ttggcattgc cccaaggctg agaatgatat gagaccgggc ggaacataca
    11521 aggcaagaat ggaagcaaaa gacggaagct tcggttttga ttttgaagca atatatacgg
    11581 atgttcaacc tggcaaagaa tttagttatg aatttggtgg gcgtaaggct tcagtctcat
    11641 tcagcaaaaa cggagaagaa acggagattg taatttgttt tgatccggaa acaacccact
    11701 ccatagaatt gcaaaggagt gggtggcaat ccattctgaa taattttaag tcatacacgg
    11761 aaaataatta actgtatttt catagttagt gctcactttt ttggccggct tgcataaaat
    11821 gaacctatag aatataccgc attttgaaat tgtaaaaaac ggatagaatc tatgttcata
    11881 ttcgaggctc ggctaaaaaa atcaaaaacc ggcaattgga acgccgcaag tttgagacac
    11941 acacgcattt gaatacaatt tcatcatcta aatgtaaaaa aggaacaccc gcaaacaata
    12001 cacttcgaag tctgagcaac ttaatttctc ttaatactga aaaaagtaaa agcttacgcg
    12061 gcatgcgctt cacctggcag gctgatcaag ttgagagttg taagtacctc aagcctgcat
    12121 tgttattaaa tgccggttta ttacagccaa tctctactct cggataactt tatgctatac
    12181 ccttagttga ttatcaaacg tacagcaggg atgttttgca attcctctaa tagtcaatat
    12241 tcttatttcc gaatccagac actatgtttt ttattaaaag atgaaagacg actatgaagt
    12301 cttggcgata aatcgttatt aaaaccctgt taagtaaata tgtaattggt ggaggtcaat
    12361 gtggatacag tttccattgt cagcgattac gactttttat tccccaatct aaaaccaatt
    12421 ttgtatctat tgtctgtaag gcgaggagat ggaagtacaa ccggacaatg aaccggtgat
    12481 gaaggttatt gaatagccaa ttttgccatt tttaccataa tttttcttaa tattgtcatt
    12541 gtaatgagcc ccaccatgaa aggccatcat caggagaggc ttccaattta ctcaaatcac
    12601 atgcatttca acccttcgac atcgacgcta tgagttcaaa atacattacg cttatcatga
    12661 tttacctggc gacaggaatc atttatctga ctgccggatt gagtaagttt tcacccggca
    12721 atataggcaa tctgatcgga ccggtcgatt atcgcaagat ctccgattcc accgtctttc
    12781 tcctcttcat gcaagggatt gccgtgcttc aagttgttgt cggagcactt ttattgtccc
    12841 agcgatattc ggtatttggg ctgatcatgg tattacctat gtccctgggc attctgattt
    12901 ttaccatcgt tgccggcttc gggcttacac ccatcatcaa tcttttgctt ttgttgcttc
    12961 tcctgtacgc attctacctg gagaaggagg caatcaggca gttattccga aaagaccgga
    13021 agggcatcct tttcaaatca ccttcatttc agcaatttcc tgaacgattg gtaccaaata
    13081 ttgccttgat tctgatcggc cttactgttc ttgtttcctt ccttgacaac attgttctca
    13141 acatacttac gaccactgcc ctgctacttt ttaccataaa cctgttccaa agaaaagact
    13201 atatctttgc cgataagatc ctcatctttc tattcctgat gatcagttct attacagtca
    13261 atggtatgtt gatcaataaa atgattgcaa aaggttttta tgttgtcttc ctgttgattc
    13321 caatgggaat tttaatttat ttgttcaggt tggtatattg gaaattttcc gaaaagaatg
    13381 tccaagaccg tcaaaatcaa gttaaggaag gatgaggtgt tgtaatcggg actatgacaa
    13441 aggccctatg aaaaggcggt atttgaccac acctcatttt ccgctgaaat ccggtccggc
    13501 atcaataaac acagcgaatg gattgcaggc aagcaattat ttattcaaat ttgaatccat
    13561 cgttacaact actcccatta aaatacgtta atattctgaa aaaaattaca gtgtgatacg
    13621 atttctgatt gcaattattt tgacaacaag tatgaatgtt gtgacgcctg cccagattga
    13681 aaccatttcg acaaaagata caacaaccta tagtgaaaaa caagccatgg cggaggatcg
    13741 ttttgtgcaa gcgacatccc tcaggctgat tggcaagtca aaagaggcca ttccgattct
    13801 tcaggaactg atcaaacaag acaaatcaga ggcgtcttat tattatgaac tcgcccgatc
    13861 gctttttgtg gaaaaagatt acgacaatgc cctgatgaat gccaacaaag ctgtctccct
    13921 tgacaaaggt aatctctttt atgccctgtt tgaggcgcag atatatgaga aacaaagcaa
    13981 tccggcaata gc
//
LOCUS       DAOQQN010000024        13636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1508_length_13636_cov_6.417421, whole genome shotgun sequence.
ACCESSION   DAOQQN010000024 DAOQQN010000000
VERSION     DAOQQN010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13636
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1508_length_13636_cov_6.417421"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1665)
                     /locus_tag="PLG24_01655"
     CDS             complement(<1..1665)
                     /locus_tag="PLG24_01655"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HMZ23615.1"
                     /translation="MYKTIAESNNFIVLDKYTKQSVMNESPGWYQSEAALEQEFIRDL
                     INQGYEYASNINSLPAMLANVREQLQSLNNMEFSDGEWSRFVEEYLDKPGENQVEKAR
                     KIHDNYIYDFVFDDGHIQNIYLVDKKYITRNKVQVISQFEQKGTHANRYDVTILVNGL
                     PLVHVELKKRDGAIREAFNQVHRYSKESFNSENSLFKYIQIFVISNGTYSRYFANTTK
                     RDKNSFDFTMTWARADNTTINDLKDFTATFFEKRVLLNVLLTYSVFDTSDTLLIMRPY
                     QIAATERMLWKIESSYNAKNWSKPESGGYIWHTTGSGKTLTSFKAARLATQLEFIDKV
                     FFVVDRKDLDNQTIIEYQRFSPDSVNGSDSTAGLKRNIEKDDNKIIVTTIQKLNNLMK
                     TEGNLPIYQKQVVFIFDECHRSQFGEAQKNLKKKFKIFYQFGFTGTPIFPQNALGAET
                     TASVFGRELHSYVITDAISDDKVLKFKVDYNDVRPQFKSIETEQDEQKLSAAENKKAL
                     LHPERIKEISQYILQNFRIKTHRNQGSNNGFNAMFAVSSVDAAKCYY"
     gene            complement(1658..2374)
                     /locus_tag="PLG24_01660"
     CDS             complement(1658..2374)
                     /locus_tag="PLG24_01660"
                     /inference="COORDINATES: protein motif:HMM:NF023178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HMZ23616.1"
                     /translation="MIVLIKLILAHFIGDFLLQPIAWVKDKEVKKAKSPYLYTHLFVH
                     GLLVLLLLWDFNYWLLALLLMISHGIIDALKLYAQNENNKTHWFLKDQILHIISILGL
                     WMLFFKPETDISALLSAPKIWIYTSALLFLTVVSGIVMKELMSSWSKTLGDDNDESLS
                     NAGKYIGMLERIFVFIFVVTGNWEGIGFLLAAKSVFRFGDLKESKDRKLTEYILIGTL
                     LSFGIAVGIGMLVLKLIENV"
     gene            complement(2371..3015)
                     /locus_tag="PLG24_01665"
     CDS             complement(2371..3015)
                     /locus_tag="PLG24_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23617.1"
                     /translation="MVAIIKGDIIASRKLVNQAKWLLPLKSLLATWGENPKNWLIHSG
                     DFFQIEIKNPGEALRKAFEIKALIKKVEPLNENKKISTIDVRMAIGVGEKNYAAESIA
                     ESNGSAFIYSSEKFELLKKENSTMGIKSPWKSFDDEMNLYLKLAGIFMDKWTVSSAEL
                     VQIIFNNPGITQEEIGKKLGIKQNSVSGRWNRANVNEVLELEAIYRNKIRSLLQ"
     gene            complement(3106..4290)
                     /locus_tag="PLG24_01670"
     CDS             complement(3106..4290)
                     /locus_tag="PLG24_01670"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HMZ23618.1"
                     /translation="MNNRTKLLKDEKVQWKTLGEVAELKRGTTITAKSKTEGNIPVIS
                     GGQKPAYYNGEFNRDGETITVAGSGAYAGFVMYWNEPIFVSDAFSIKANQEIVLPRYI
                     YHFLLSIQSQIHDLKSGGGVPHVYAKDVARFKIPIPPLHIQQEIVAILDKFTELTAEL
                     TAELTARKKQYDYYREQLLSVDDKGLINGVKAEWKTLGEVGELVRGNGLPKSDFTETG
                     IPAIHYGQIYTYYKTHTTETISFVSPETAEKLQKVNYGDVVITNTSENIEDVGKSLVY
                     LGQEQAVTGGHATIFKPSNEILGKYFAYFTQTEIFASQKRKLAKGTKVIDVSAKDLAK
                     IKIPIPPLSEQELIVSILDKFDTLTTSISEGLPKEIELRQKQYAYYREQLLDFPKNNI
                     ES"
     assembly_gap    5134..5233
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5609..6004)
                     /locus_tag="PLG24_01675"
     CDS             complement(5609..6004)
                     /locus_tag="PLG24_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMZ23619.1"
                     /translation="MHSSWEEELYKYISGIVSNKEQKMLAINGMPDHIHFLIGMKPGC
                     CLSDLVREIKKSTNDFIKGKKFTPFKFQWQEGYGAFSYSHSALDNVIAYIKNQKEHHK
                     KQTFKDEYKSFLTKFQIDYKDEYLFEWLE"
     gene            complement(6115..7662)
                     /locus_tag="PLG24_01680"
     CDS             complement(6115..7662)
                     /locus_tag="PLG24_01680"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HMZ23620.1"
                     /translation="MTSLAQRQELQNKIWKIANDVRGSVDGWDFKQFVLGTLFYRFIS
                     ENFTNYIEGGDESIDYSKLSDTVITPEIKDDAIKTKGYFIYPSQLFVNVAKTANTNPN
                     LNTDLKAIFDAIESSANGYPSESDIKGLFADFDTTSTRLGNTVEAKNSRLAAVLKGVE
                     ELSFGNFEDNQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHKQDKV
                     NKIYDPACGSGSLLLQAKKHFDNHIIEEGFYGQEINHTTYNLARMNMFLHNINYDKFN
                     ITLGNTLLNPHFGDEKPFDAIVSNPPYSIKWIGDDDPTLINDDRFAPAGVLAPKSKAD
                     FAFVLHALSYLSGRGRAAIVCFPGIFYRGGAEQKIRKYLVDNNFVETVISLAPNLFYG
                     TSIAVNILVLSKHKTDNKTRFIDAGGEDFFKKATNNNVLEDAHIDKIMQLFDSKADHD
                     HVAVSVDNARIAENDYNLSVSSYVEAKDNREVIDITELNKEIAETVEKINKLRVDIDE
                     IVKEING"
     gene            complement(8314..9213)
                     /locus_tag="PLG24_01685"
     CDS             complement(8314..9213)
                     /locus_tag="PLG24_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23621.1"
                     /translation="MGDYSDLIRLDAIALGDLAFSIGDYATAVDNYKEALEYLGYYHG
                     DNQDFLMLEAGLVVRIEELEKSLDSRDRLLYPESWTLSKTSFVKGRQCVKYLYLDRYK
                     KSDRTPLSKETLRLFDKGHAFEERFRAEFFPEGTNVKEDVRRFSYFKSYTDYLLQLSG
                     RQVLFEAAIIEEEVLILCDVLVKEDNGDVEVYEVKMTTSLNEAILNDVALQYFICRKK
                     FGDNLKKFNLVLPDESDASGFQIVDLAEILNSKIFPVAEQIAQLKDVLKRSEPDVIMG
                     SQCHSPYRCEFVEYCTSRAQKQQ"
     gene            complement(9665..11209)
                     /locus_tag="PLG24_01690"
     CDS             complement(9665..11209)
                     /locus_tag="PLG24_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23622.1"
                     /translation="MRHVFFTIFTMLFTQFIFAQSVDLDKEQIKVSYVKLPEKPVLDP
                     ALRTYYIKASGSSLDINQLKKGVSIMGWTKNTSNGFYIVDLGEPKVRLFTANTIEETD
                     LKDGKKVVTGYYPKIEYTLEFEAKVTDSTGKLIKKIPYTSRVTTFNGAKVKTRKDSDA
                     KLSLKDPDFLKEVGGFLVRVVNAQLNNDFGYEIINKNEVLWILNSKKHPDYEGSKNAY
                     ATVKSAFDKMAYDKPTDQLRAELEPAIKYFKEMPDRYPGTDKKERKMKYSAYFNLAYI
                     YYFLDDPEQAIMWADKLIANDYDPKDGAKIKEDAQKLGPVMKANMTNSRHMPVSTIDN
                     LKIEIEEPSVSIKNMERYNPVVIRIKKDSQIVHGFLPKDFDEVIASANAYISLVVVKE
                     GKEEDELFLLAAIDYVKVAEGNTYRAVFARENDKRRIVLAKELYNKGKYKVFELGESG
                     ILFQHLDEDANLFALGTWVLSYKKFYAFMAKDCPAVRKMVENKEFGVSEEKAIKFCMA
                     MDEHCK"
     gene            complement(11565..>13636)
                     /locus_tag="PLG24_01695"
     CDS             complement(11565..>13636)
                     /locus_tag="PLG24_01695"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HMZ23623.1"
                     /translation="NEEVIYGEENARLYKLRYVRTDDPGKALVISTQRPETIMADTAI
                     AVHPDDERYRDWVGKKVLIPLINKEIPVIADDYVDIEFGTGALKVTPAHDINDYELGK
                     KHNLEIIDILNDDGTLNEKATILIGQDRFEARKNIITLLEEACLLEGTIDYRTNIGRS
                     ERTNAVVEPKLSLQWFVDMKALAGPALKAVNDGDINFYPKNQVNTYNHWLENIRDWCI
                     SRQLWWGHRIPAYYYADDVFVAARREEAESLAKAKIGADFDAAKLRQDEDALDTWFSS
                     WLWPITVFDGFSDGKDYGYYVPTSVLVTGWDIIFLWVARMIMAGYEWKEERPFKDVYF
                     TGMVRDKQRRKMSKSLGNSPDALGLIDMYGADGVRFGMMSSSPAGGDLLFDEKLCEQG
                     RNFCNKIWNALRLITSWEVSDTSDASVRNKNKLACDFMRQRISEVQTQTEANFDAYRL
                     SENLILIYSLIWDDFCAYYLEMIKPTYGQPVDRETLVEANALFADLMIILHPFMPFIS
                     EEVYQQVKMKDMPDCIVARYPTPAFKNDVMVEGFAFFKDFVSLIRDIRNRNGLKQRDI
                     LPVYPEGDISSYLSLSGYRELMESIAYVELRSEDNLDAERSVTSLLRTVKIFVELPHV
                     AGNKAEAQAEILEKIKYFEGFVASINKKLENQRFVENAPAEVIEKERKKLADSLASIE
                     ALKKELK"
BASE COUNT         3552 a         2894 c         2644 g         4446 t
ORIGIN      
        1 ataataacat ttggctgcat ccacactact tacggcaaac atggcattaa aaccgttgtt
       61 gcttccctga tttctgtgtg tttttattct gaaattctgt aagatgtatt gagatatttc
      121 tttgatacgt tccggatgaa gcaaggcttt tttgttttct gctgcgctca gtttctgctc
      181 gtcttgttcg gtttcgatgc tcttaaactg aggtctgaca tcgttataat ccactttgaa
      241 tttcagtact ttgtcatcac tgatggcatc cgttattaca taagagtgta attcacgacc
      301 aaagacactt gccgtagttt cagcgcctaa agcattttgc gggaaaatag gcgttcctgt
      361 aaaaccaaac tgatagaata tcttaaactt ctttttcagg ttcttttggg cttccccaaa
      421 ttgtgaacga tggcattcat caaaaatgaa aactacctgc ttttgataaa ttggcaggtt
      481 accctccgtc ttcatcaggt tgtttagctt ttgtatggtg gtaacgatga ttttgttgtc
      541 gtccttttca atatttcgct ttaaacctgc tgtactgtcc gaaccattca cgctgtcggg
      601 agaaaaccgc tgatattcta ttatggtctg attgtccagg tctttacggt caaccacaaa
      661 aaatacttta tcgataaatt ccaattgtgt ggctaaacga gccgccttaa aactggtcaa
      721 tgttttaccc gaacctgttg tgtgccatat atagccgcca ctttcgggct tcgaccaatt
      781 tttggcgtta taggaacttt caatcttcca caacattctt tcggtggctg cgatctgata
      841 cggtcgcata atcaaaaggg tatcgcttgt atcaaaaacc gaataggtga gcaacacatt
      901 taacaatact cttttttcaa aaaatgttgc tgtaaaatct ttcaggtcat taatcgttgt
      961 attgtcagct cttgcccaag tcatggtaaa atcgaaactg tttttatcac gcttggtggt
     1021 gttggcaaaa taacgactat aggttccgtt tgaaatcaca aatatctgga tgtatttaaa
     1081 gagtgaattt tcactgttga aactttcttt gctgtagcga tgcacctggt tgaacgcttc
     1141 acgaatagca ccgtctcgtt tttttagttc tacatgcacc agaggcaggc cattgacaag
     1201 gatggttaca tcatagcgat tggcatgtgt gcctttttgt tcaaactgag aaattacctg
     1261 tactttattt cgggtgatat actttttgtc caccaggtag atgttttgaa tatggccatc
     1321 atcaaagacg aaatcgtaga tatagttgtc atgtattttt cttgctttct caacctggtt
     1381 ctcgccgggc ttgtccaaat attcttccac aaaacgcgac cattctccat ccgaaaattc
     1441 catattgtta agcgattgca attgctcccg aacattggcg agcatagcag gaagtgaatt
     1501 gatattactc gcatattcat aaccttggtt tatcagatcc cggataaatt cttgctccaa
     1561 agctgcttct gattgatacc aaccaggaga ttcattcatc acagattgtt tggtgtattt
     1621 atccagtacg atgaagttgt ttgattctgc tatggtttta tacattttca attaatttaa
     1681 gtaccaacat tcctatacct acagcaattc caaagctcaa taatgttcct attaaaatat
     1741 attcggtcag ctttctgtct tttgattctt ttaaatctcc aaagcgaaat actgattttg
     1801 cggccaataa aaacccgata ccttcccagt ttcctgtcac cacaaaaatg aaaacaaaaa
     1861 ttcgttctaa catgccaatg tatttaccgg cattgctcaa tgattcatca ttatcatcac
     1921 ccaatgtttt agaccagctt gacattaatt ctttcattac aatgccggat actaccgtta
     1981 agaaaagcag ggcacttgta taaatccaga tttttggagc ggataacagt gcgctaatat
     2041 ctgtttcagg cttgaaaaat aacatccaca agcctaagat gcttataata tgcaatatct
     2101 ggtcttttaa aaaccaatgg gttttattgt tttcgttttg ggcatataat ttaagggcat
     2161 caatgatacc gtgtgagatc atgagtaata aggctaatag ccagtaatta aaatcccata
     2221 ataataacaa cacgaggaga ccgtgtacga ataggtgtgt atacaggtat ggggatttag
     2281 ctttctttac ctctttatct tttacccatg ctataggttg tagtagaaaa tctcctataa
     2341 aatgtgccaa tatcaatttt atcagtacaa tcattgtagt aaagatctaa ttttgtttct
     2401 gtaaatagct tccagctcca gtacttcatt tacattggct ctgttccatc ttccgctaac
     2461 ggaattttgt ttgatgccca gctttttccc gatttcttcc tgtgtaatgc ccggattgtt
     2521 aaaaatgatt tgcaccagct cagccgaaga aacggtccat ttatccataa aaattccggc
     2581 cagttttaag tacaaattca tttcatcgtc aaagcttttc catgggcttt ttatacccat
     2641 tgtgctgttt tcttttttga gtaattcaaa cttttcgctt gagtaaataa aggccgaacc
     2701 attactttct gctatgcttt ctgctgcgta gttcttctct cctacaccta tagccattct
     2761 tacatctata gtgcttatct ttttgttttc gttaagcggc tctacttttt tgattaaggc
     2821 ttttatttcg aaggcttttc gaagagcttc cccgggattt ttaatttcta tttgaaaaaa
     2881 atctccggaa tggataagcc agtttttggg gttttcgccc caggttgcca gcaaactttt
     2941 gagtggcaac aaccattttg cctggtttac caactttctt gatgcgataa tatctccttt
     3001 aataattgct accatagtgc aaatatcgtt taaatgaacg atatttcaaa atatcgttta
     3061 aataagcgat aatttaaaat atcgtggatt gttgtgatgt cctttttatg attctatatt
     3121 attctttgga aagtcaagca actgctcccg ataatacgca tactgctttt gtctcaactc
     3181 aatttctttg ggcaagcctt cgctgatgct ggtggttaag gtatcgaatt tatctaaaat
     3241 agaaacaatg agttcttgct cggagagggg tggaatgggg attttgattt ttgctaaatc
     3301 ttttgcagaa acatcaataa ctttagttcc tttcgctaat tttctttttt gacttgcaaa
     3361 aatttccgtt tgcgtaaaat aggcgaaata ttttcccaga atttcattac ttggtttaaa
     3421 tatcgttgca tgcccgccgg taacagcttg ctcttgccct aggtacacta atgactttcc
     3481 gacatcttca atattttcac tagtgtttgt aattacaaca tcaccataat ttaccttctg
     3541 taatttttca gctgtttcag gagaaacaaa tgaaatagtt tctgttgtgt gtgttttata
     3601 gtatgtgtaa atttgaccat aatgtattgc agggatacct gtttcggtaa agtctgattt
     3661 tggcaatccg tttcctctta ccaattcccc cacttccccc agcgttttcc attctgcttt
     3721 tacgccattt attaagcctt tatcatctac gcttaacaat tgctcccggt agtaatcgta
     3781 ttgtttttta cgagctgtaa gctctgctgt aagctctgct gtaagctcgg tgaacttatc
     3841 caaaatagca actatttctt gttgaatgtg gaggggcggg atggggattt tgaaacgagc
     3901 cacatctttt gcatatacat gaggaacgcc acctccagat ttcaaatcgt gaatttggga
     3961 ttgaatactc aataagaaat gatagatata tcgaggtaaa acaatttctt gatttgcttt
     4021 aattgaaaac gcatcactta caaaaatcgg ttcattccaa tacattacaa aacctgcata
     4081 agcacccgaa cctgcaacag taatggtttc tccatctcta ttaaattctc cattataata
     4141 tgccggtttt tgacctccgc taattacagg aatattgcct tctgttttac tttttgccgt
     4201 aatagttgtg cctcgtttca gctccgccac ttccccgagc gttttccact gcactttttc
     4261 gtcttttaat aattttgttc tgttgttcat tattaaattg tttgttattg cttaaaatcc
     4321 ccgaagggat taaatgttta tgaaatcaat gatttggttt tattcgaccc cgttggggtc
     4381 gcaccatcta taaacatttg gttctataaa catttgacct cttcgaggtc aggcccacat
     4441 cccatttttt ctataaacat ttgaccctat tggggtctgt tgttttccaa tctgcggttg
     4501 taatttgtaa taatgttgtt atgttttgca ttgtatgaat attgaattaa tcaaatcccg
     4561 gatgggttaa atgtatatga aatcaatgat tcggatttat tcgacccatt cggggtcgcg
     4621 catctataaa cattcggttc tataaacatg tgacctcttc gaggtcagac ccatatgaca
     4681 ttttttctat aaacatgtga cctcttcgag gtctgttgtt ttctattttc cggttgtgtt
     4741 ttccatggtg ttaattttgt aataatcaaa tcctgaaggg attaaatgtt tatagaaatt
     4801 tatgatttga ttttatacga ccctgaaggg gtcgcatctt ctcccgttat cgattccata
     4861 aatattcgac cccctttggg gttgccattc tacggttgta ttttttaatt atgtaattat
     4921 attttccatt atatgaattt tgtaataatc aaatcccggc gggggttaaa tgtttatgaa
     4981 atcaatgatt cggatttatt cgaccccgtt ggggtcgcac catctataaa cattctgttc
     5041 tataaacatt tgacctcttc gaggtcaggc ccatatcaca ttttttctat aaacatttga
     5101 ccctattggg gtctgttgtt ttccaatctg cggnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnatagaaa atatgatttg attttatacg accctgaagg ggtcgcatct
     5281 tctcccgtca tcgattccat aaatattcga ccccctttgg ggttgccatt ctatggttgt
     5341 attttttaat tgtgtaatta tattttccat ggtattaatt atgtattaat caaatcctga
     5401 agggattaaa tgtttataga atttatgatt cggttttata cgaccctgaa ggggtcgcat
     5461 attgattgtc tttgtttttc tataaacatt tgacctcttc aaggtcaggc ccatatccca
     5521 ttatcttttc tataaatata cgacctcttc gaggtcacat ctcccaatat tttgtttttt
     5581 aaaaatcatt tgacctcgaa gaggtctgct attccaacca ttcaaacaaa tattcgtctt
     5641 tgtaatcgat ttgaaatttg gttaaaaagg atttgtattc atctttaaat gtttgcttct
     5701 tatgatgctc tttttgattt ttaatatatg caattacatt atccaatgct gaatggctat
     5761 atgaaaatgc accatatccc tcttgccatt gaaatttgaa aggggtaaac ttcttcccct
     5821 taataaaatc gtttgttgat ttttttatct cccttaccaa atcggataaa caacaaccgg
     5881 gtttcattcc tattaaaaaa tgaatatgat cgggcattcc attaatggcc aacatttttt
     5941 gttctttgtt gctaacaata cctgaaatat atttatatag ttcttcctcc caggaagaat
     6001 gaattaggct ttctctacct taaacggcaa agactacctg aatataaatt tgtgaaaatg
     6061 ttcctgccat aaaacttatt tttaaactgt gaatacttgt tttttatccc atcattatcc
     6121 atttatttct ttaactatct catcaatatc tactctcaac ttgttgatct tctctaccgt
     6181 ttcggcaatt tccttattca attccgtaat gtctatcact tcccgattgt cttttgcttc
     6241 tacataagaa cttaccgaga ggttgtaatc gttctccgca atccgggcat tgtcaacaga
     6301 aacggcaaca tggtcatggt ctgccttgct gtcgaagagt tgcatgattt tatcgatatg
     6361 cgcatcttcc aacacgttgt tattggtggc ttttttgaag aaatcttcgc caccggcatc
     6421 gataaaccgg gttttgttgt cggttttatg ttttgatagc acaaggatat ttaccgcaat
     6481 ggaagtacca taaaacaagt taggagccag ggaaattacg gtttcaacaa agttgttgtc
     6541 caccaggtat tttctgattt tctgctctgc accaccccgg taaaaaatgc cggggaaaca
     6601 aacaatggcg gctctgcccc taccggataa atagcttagg gcatgaagca caaaggcgaa
     6661 atctgccttt gattttgggg ccaacacccc ggcaggagca aagcggtcgt cattaatcag
     6721 tgtcgggtca tcgtcaccaa tccatttgat agagtaaggc ggattggaaa caatggcatc
     6781 aaagggtttt tcatcgccaa agtgtgggtt gaggagcgtg tttccaaggg tgatattaaa
     6841 cttgtcgtag ttgatgttgt gtaaaaacat gttcatacga gccaggttgt acgtagtatg
     6901 gttgatttcc tgtccataga agccttcttc aataatatga ttgtcgaaat gttttttggc
     6961 ttgcagcaat aaagaacctg aaccgcaagc cgggtcgtaa atcttgttta ccttatcctg
     7021 cttgtgcatg gccaattgtg cgatgagttt ggacacatgc tgaggggtga aaaactctcc
     7081 gcccgactta ccggcattgg cggcatagtt ggaaatcaaa aactcataag catcaccaaa
     7141 aaggtcaatt tgattgtctt caaaattacc gaaactcagt tcttcaacac ctttcagcac
     7201 cgcggccaag cggctgtttt tggcttcaac ggtatttccc aggcgtgtgc ttgttgtgtc
     7261 aaagtcggca aataaacctt taatatccga ttctgatgga tagccatttg ccgaactttc
     7321 aatggcatcg aaaatggctt ttaggtcggt gttgaggttg gggttggtat ttgccgtttt
     7381 agccacattc acaaataact gacttggata gatgaagtaa cctttggttt ttatggcatc
     7441 gtcttttatt tcgggagtga ttacggtatc agacaatttt gaatagtcaa tgctttcatc
     7501 acctccttca atgtaattgg taaagttttc actgatgaag cgatagaaca gtgttcctaa
     7561 aacaaactgt ttaaagtccc acccatccac agagccacga acatcgttgg ctattttcca
     7621 aattttattt tgtaattcct gtcgttgtgc taagcttgtc attttttctc tttaaaataa
     7681 gtcttttggt tttatgcacg atattttcgc aatttcaaaa agttcttcaa gttccggctg
     7741 ttgccggttt taaaatattg ccgtttatca cggtcgtaac ttttatacgg tttttacgcc
     7801 aaccgggttt tgttctattc ttttatcttc cggcagttct ttgattctgt tcatgtcatt
     7861 ttgaaatgtg cccgtaaaga tagttttaat aaccgggcca tctcattttt caatataaaa
     7921 accgtttgaa ttattgttgg tctgtctttc ggtgcggagg caaaaatggc actttgggca
     7981 ttgtgaaggg taggcttgtg cgtcgggcaa aaaaatgtga catttgagtg gtggcaaaaa
     8041 aaatggaatg gtacctcccg gagaaaaggc ccatataata gacccttggg cttatcgcac
     8101 ctgctatcag aacttgtcaa ccgttacgga catgaatttt ccggacaacg cctctgagca
     8161 ttgtcgttat cagggtgttg agtcgcgttc ttacagcggg tcaatcatgc cttttagaca
     8221 ggatcgaccg tgcttaggta attctgcgac cgcgaactcc ctgtatgata gcgtacaatc
     8281 ggacgatgag taccggatcg gcacatggca ggcctattgt tgcttttgtg cccttgatgt
     8341 gcagtattct acaaattcac atcgataggg agaatgacat tgagagccca ttataacatc
     8401 cggctcgctt ctctttagga cgtcttttaa ttgtgcgatt tgttcggcga cagggaatat
     8461 ttttgaattg agtatttcag caaggtccac gatctgaaag ccgcttgcat ctgattcatc
     8521 cggcaatacg aggttaaatt ttttcaaatt gtcgccaaac tttttcctgc aaataaagta
     8581 ttgtaaggca acgtcattga gaatggcttc attgagcgag gtcgtcattt ttacttcata
     8641 tacttctaca tcaccgttat cctcctttac caggacatcg caaaggatca acacctcttc
     8701 ttcaatgata gccgcctcga aaagcacctg cctgccggac aattgcagca aataatcggt
     8761 ataggattta aaataggaaa atcgccgtac gtcttccttg acattggttc cctcgggaaa
     8821 gaattctgct ctgaaccgct cttcaaaggc atgacccttg tcaaaaagcc taagggtttc
     8881 ttttgagagt ggcgtgcggt cggatttttt atatcggtca agatagagat acttcacaca
     8941 ttgtcttcct ttgacaaaag atgtttttga cagggtccat gattccgggt acaacagtcg
     9001 atctctcgaa tccaaagatt tctccaactc ttcaatccgg acgaccagcc ctgcttccag
     9061 catcagaaaa tcctgattat ctccgtgata gtagcctaaa tattccaggg cctctttata
     9121 gttgtccacg gcagtggcgt agtctcctat actaaaggcc agatcgccca aagcaatagc
     9181 atccaaccga atcaagtcac tgtaatctcc cattttcttt tcttcaatta tctcatggaa
     9241 aaggcattat ccgcggattg ccggatcgat tccctccggg tctttatccc ggacttcagt
     9301 ttgaaagacg ttgattttcg ggaaaagttc aacaggtaaa tattttttca ttcagaaggc
     9361 aaacatcggt tgtgacaaaa acatcaggga aggggcatct ttatgccata taaatgcagc
     9421 gaatcaaacc actcagagga atccctcccg ccgcgaaaaa aattgcagcg gcaatctcaa
     9481 tcggctgagt tatcaaggat attcgattgt ccttgacggc cgggtaattt catcctcccg
     9541 acgtataaac ccataataca aatgattgaa tacgctcaag aaccaataac cgcagttttc
     9601 atcaagaaaa gcagaggatg cacgcatggt gtttcgtgta tcccctgctc tttgtatacc
     9661 gatcttattt acaatgctca tccattgcca tacagaactt aattgctttt tcttcagaaa
     9721 cgccgaattc cttgttctca accatcttcc ggacagctgg acagtctttt gccatgaaag
     9781 catagaactt tttgtagctt aatacccatg ttcctagagc aaacagattg gcgtcttcgt
     9841 ccagatgctg gaagagtatt ccgctctcgc ccagctcaaa gactttatac ttacctttat
     9901 tgtacaactc ctttgccagc acaatccttc ttttatcatt ttccctggcg aatactgccc
     9961 gataggtatt accttccgcc actttgacat agtcgatggc tgcaaggaga aaaagctcgt
    10021 cttcttcctt cccctccttt actacgacaa gacttatata ggcatttgca ctggcaatga
    10081 cttcgtcaaa atccttgggc aaaaagccat ggactatttg gctgtctttt ttaattctaa
    10141 tgaccacggg attgtatcgc tccatatttt ttattgacac ggagggctct tctatttcta
    10201 tctttaggtt gtcgatggta ctgacaggca tgtgtcttga attggtcatg ttggccttca
    10261 tgacaggccc cagtttttgg gcatcttcct ttattttagc cccgtctttt ggatcatagt
    10321 cattggcaat cagcttgtct gcccacatta tagcctgttc cgggtcgtct aaaaaatagt
    10381 aaatataggc caggttgaag tatgccgaat acttcatttt tctttccttc ttatcggtcc
    10441 cgggatagcg gtccggcatc tctttaaaat acttgatggc aggttcgagt tctgcgcgaa
    10501 gttggtcagt cggcttgtca taagccatct tgtcaaatgc tgactttaca gttgcatagg
    10561 cgttcttgct accctcataa tcagggtgct tcttactgtt caaaatccaa agtacttcgt
    10621 ttttatttat tatttcatag ccgaagtcat tattgagttg agcattaaca acccgtacca
    10681 ggaacccgcc gacttctttt agaaaatccg gatccttcag tgataatttg gcatcggagt
    10741 ccttcctggt tttgaccttt gctccattga aggtggttac tctggaagta taggggattt
    10801 ttttaatcag cttaccggtg ctatccgtga cttttgcttc aaattcaagg gtgtattcta
    10861 ttttgggata atatccggtc actacttttt tcccatcctt gagatctgtc tcttcgatgg
    10921 tgttggcggt gaataacctg accttgggct ctcccagatc cacaatataa aatccgttgg
    10981 aggtgttctt ggtccacccc atgatggaaa cgcccttttt taattgattg atatcaagag
    11041 aagagcctga agccttgata tagtatgtcc tgagcgccgg gtcgagaacg ggcttttccg
    11101 gcaactttac ataggatacc ttgatctgct ctttgtcgag atcaacagat tgggcaaaga
    11161 tgaattgggt gaaaagcata gtgaaaattg taaaaaaaac gtgtctcata aggctttgtt
    11221 ttttagtgtt taaaaaagtt aatgaggagg catgacagaa acatgcccgg acaatgcgga
    11281 ttatgcacca ttcaggggca acggactata caatgccttg gaaatattta tgtcttaaaa
    11341 atacaattct atttttgaat tgtatgtatg gacaacaaaa acattaaaaa aaaaacatgc
    11401 aatcagggtc aattcctctc ttgcaaaagg aagtacgtgt cagataacgc ttaaaaatat
    11461 ttattaagca tgaacgggca aaagccattc tttccaacaa ttacggtttt ttgtgcctgt
    11521 atgggcaaat taacctatcg aatcaccatg aaatgcaaag tgcactactt cagctccttc
    11581 ttcaatgctt ctatgctggc cagactatct gcaagcttct tcctttcctt ctcgatgacc
    11641 tctgcaggtg cattttcaac aaaccgttga ttttcgagct ttttgttgat ggaagctaca
    11701 aagccttcga aatatttgat cttttccagg atctcggctt gagcttccgc cttattcccc
    11761 gctacatgcg gcaactcgac gaaaattttg acggtccgta aaagcgatgt tacggagcgc
    11821 tcagcatcca gattgtcttc agagcgtaat tccacgtatg caatggactc catcagttca
    11881 cggtaacctg acaaagacag gtagctgctg atgtcccctt cgggatagac cggaaggatg
    11941 tcacgttgct tcaggccgtt tcggtttcgg atatcgcgga tcaatgaaac aaaatctttg
    12001 aaaaaggcaa atccttccac catcacatca tttttgaatg caggtgtcgg atacctggct
    12061 acaatacaat ccggcatgtc tttcatcttg acctgttgat acacttcttc ggagatgaat
    12121 ggcataaacg gatgcaggat gatcatcagg tcggcaaaca aggcattggc ctcgaccagg
    12181 gtctcacgat ccacgggctg accataggtt ggcttaatca tctccagata ataagcacaa
    12241 aaatcatccc agattaaaga gtagatcagt ataaggttct ctgaaagcct gtatgcatca
    12301 aagttggcct ctgtctgtgt ctgaacctcg ctgatacgct gtcgcatgaa atcacaggcg
    12361 agcttgtttt tatttctgac tgaagcatcc gaggtatcag aaacctccca ggaggtgatg
    12421 agccgcaggg cgttccatat cttattgcag aaattacgtc cctgctcgca taatttctca
    12481 tcgaacagga ggtctcctcc ggccggagag gacgacatca taccgaaacg cacaccatcg
    12541 gctccataca tgtcgataag gcccagggca tcgggcgaat taccgagcga cttgctcatc
    12601 ttacgacgct gtttgtcccg taccattccc gtaaaataca cgtccttaaa gggcctttct
    12661 tccttccatt cgtaaccggc cataatcatt cgggccaccc agaggaagat gatatcccaa
    12721 ccggtgacca atacacttgt cggcacataa tagccgtaat ctttgccgtc agagaagccg
    12781 tcaaatacgg tgattggcca cagccaacta gagaaccatg tgtccagggc atcttcatcc
    12841 tgccgcagct tagcggcatc aaaatccgca ccaattttgg cctttgccag agactcagcc
    12901 tcttcgcggc gagcagccac aaacacgtca tcggcataat aataggccgg tatgcgatgt
    12961 ccccaccaca actgacggct aatacaccag tcccgtatgt tttccagcca gtggttgtag
    13021 gtgttgacct gatttttggg ataaaagttt atgtcgccgt catttactgc tttcagggct
    13081 ggtcccgcca gcgccttcat atcgacaaac cattgcaggc taagcttggg ctccactacg
    13141 gcattggtgc gttctgatcg tcctatattg gttcggtagt caatggtgcc ttccaataaa
    13201 caggcctctt ccagaagcgt aatgatgttt tttctcgcct caaagcggtc ctggccaatc
    13261 aggatggttg ccttttcgtt gagggtgccg tcatcattga ggatgtcaat gatctcaagg
    13321 ttgtgtttct tgccgagttc atagtcgttg atatcatggg cgggtgtgac ttttaatgct
    13381 cccgttccga attcgatatc tacataatca tcggcgatga cagggatttc cttattgatg
    13441 agggggatca ggactttctt acctacccaa tcccggtatc gttcatcgtc agggtgtaca
    13501 gcgatggcgg tgtcagccat gatggtttcc ggacgctgag tggagatgac gagggccttg
    13561 cccggatcat cagtccggac atagcgcagt ttgtacagac gcgcattctc ttcaccatag
    13621 ataacttctt cgttgg
//
LOCUS       DAOQQN010000025        13574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1517_length_13574_cov_5.125823, whole genome shotgun sequence.
ACCESSION   DAOQQN010000025 DAOQQN010000000
VERSION     DAOQQN010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13574
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1517_length_13574_cov_5.125823"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1074)
                     /gene="rlmN"
                     /locus_tag="PLG24_01700"
     CDS             complement(40..1074)
                     /gene="rlmN"
                     /locus_tag="PLG24_01700"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HMZ23624.1"
                     /translation="MTNDIRLISPSQLEEWLKQQGEPRFRAGQISEWIWQKQALSFDE
                     MTNLPLALREKGKAEFVFRPIEEDFVQTSRDGTIKSRFKLHDGHMIESVLIPVPDEKR
                     FTACVSSQVGCSLACKFCATGMMGRIRNLDFPEIYDQVVRVNRQALEHFGHPLTNIVY
                     MGMGEPLLNYSNTLRSIDIITSPKGLGMSPSRITISTAGIAKMIRRLADDGVKTNLAL
                     SLHAANDGKRNEIMPINDQNGIDILMDALRYFYRKTRNRITYEYLALQNFNDNEEDAV
                     QLAALTRDFPVRVNIIEYNTVEGLAFHKTTEDKLDKFVRTLNQHHVNVTVRRSRGKDI
                     DAACGQLANK"
     gene            complement(1543..2496)
                     /locus_tag="PLG24_01705"
     CDS             complement(1543..2496)
                     /locus_tag="PLG24_01705"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ23625.1"
                     /translation="MVDTQHEFLQRNDHAGNDYALFYTIYQRKDIVPGATLLVLHGMQ
                     EHSGRYDEFARYMAERGFVVLTYDHRGHGKTARNMSEMGFFRKNKPAQLLLEDVRAMK
                     SLLEGQFPGLPHFVLGHSMGSFILRTFLRQEKSDFQGIILVGTGSRTPGAILIKAGLH
                     LFNVLSPKSRPSFLNNIFGTINNARFRHEVNAGATSWLSVSMDNRNAFEQDPQCGVPF
                     TVNGFLGLLTVHVRATSPEWSGNLPKDQPILFVGGSDDPIGDFGKGVLKVARHLEKAG
                     FSDTTIKLYSGMRHEILNEDIRLEVFEDILQWMGNRVRIKI"
     gene            complement(2904..3707)
                     /locus_tag="PLG24_01710"
     CDS             complement(2904..3707)
                     /locus_tag="PLG24_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23626.1"
                     /translation="MLILLGLCLVFATSCQKTDKRDYTGTYVGHIRGKSLSMSLSQPD
                     KTTIKGTIFDRRSTYLIHGEIIGDGIKATATDTVAKTEFSIDGSWKGDTLTLMMAMTK
                     PMQTTRSFPVNFLKVVISTNQKGIDELADLGLLQSSGGRDTANGINIEANKSGLNAGK
                     VKSDSEITEHNFPGLWQIVSIKGKASTAFRQDAYYLFNSDKSILKLDRTAVPLDGYYW
                     NIGENKLFLFYKINGRMDEVRPDIIEYSNDLLRVKDGESEIELKKYTKG"
     gene            complement(3739..5295)
                     /locus_tag="PLG24_01715"
     CDS             complement(3739..5295)
                     /locus_tag="PLG24_01715"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HMZ23627.1"
                     /translation="MIRIIGGLLLAGCSVLYGQNTPPVTVPLHVSHDAANHRITITYD
                     LMDVESDTATVVLQQKTGSGNFLTASGVTGEVGSGILPGEAKSIIYSYPSGTDLRNVS
                     LRLLAYDRHTPSIQEMVDKVDISNLQKMLGDIVGVRHYKSNPNHLIYVGNYINQYMID
                     HGLSIHHQDFTFSGLSFSNLIGRLEGLNNNKLCLINGHFDSAEFAPGADDNGTGVVGV
                     LEAIRVMSDYLFENNVQFTSFNLEELGLIGSINYVSKGIEPDQQIIGLINYEMLGYYS
                     DEPNSQAIPPGFELLFPNQVVEITNDQKRANFIVNTGAETSFGLMAAFTKAARTYVPG
                     LKVIELPLPGNGTIAPILRRSDHAPFWDKGYPALMITDGAETRNPNYHQLSDKIETIN
                     FDFMAKVVKTGIATLATIAKPLNGSTSGIDLSSILSTNTIGALEAITIMPNPVKDVLS
                     FNYISGTNNGKFTAEVISDTGHKILKRVMDKDASGRYSLNIGSRTAGQYHLHIIDGHN
                     RTVARFMIIE"
     gene            complement(5385..5903)
                     /locus_tag="PLG24_01720"
     CDS             complement(5385..5903)
                     /locus_tag="PLG24_01720"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HMZ23628.1"
                     /translation="MADFIIRPWVLADLTSLVKYANNFNISRNLTNHFPFPYTEDAGK
                     IFIEFAMAGRPNHIFAIEIDGEAAGGIGIHPQSDIFLKNAELGYWLGEPFWGRGIISN
                     AVRQMVDFGFRTFEIDRIFARPFGTNIASQKVLEKNHFKLEGRFDKVIYKNGAYQDEL
                     VYAIRRENWLKI"
     gene            complement(6049..7806)
                     /gene="aspS"
                     /locus_tag="PLG24_01725"
     CDS             complement(6049..7806)
                     /gene="aspS"
                     /locus_tag="PLG24_01725"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HMZ23629.1"
                     /translation="MYRTHNCGELRLSDAGNNVTLSGWVSVSRDFGGLTFLDLRDRYG
                     ITQLVFDADSNPEIREKAQKLGREYVIRVAGKVRERSNKNPNRATGEIEIDVDTLHIL
                     NASRIPPFTIEENTDGGDDLRMKYRYLDIRRDPVKNNLIFRSNLAIETRKYLADEGFI
                     EVETPFLIKSTPEGARDFVVPSRMHPGSFYALPQSPQTFKQLLMVSGLDKYFQIVKCF
                     RDEDLRADRQPEFTQVDCEMSFVTQEEILNTFEGLTRHLFQSVLQIDLGTFPRMTYDE
                     AMHRFGSDKPDMRFGMEFNYLKEAVSGRDFGVFDSAETVIGICAKGCESAFSNKDLKE
                     LTEWVQRPQIGAKGLVYVKYGADGIKSSVGKFYSEEDLLRIGKECGAETGDLLLILSG
                     ELEKTRKQLNELRLEMGRRLGLMREGDFKPLWVVDFPLLEWDEETQRFYAMHHPFTSP
                     KMADLEKMQSDDKEVLNSLRADAYDLVINGSEIGGGSIRIFDRELQAKNFRLLGFSPE
                     EAERQFGFLMNAFEYGAPPHGGIAFGFDRLCAVMSGSSSIRDFIAFPKNNQGRDMMID
                     APATIDGGQLKELGLKLDS"
     gene            8104..9522
                     /locus_tag="PLG24_01730"
     CDS             8104..9522
                     /locus_tag="PLG24_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23630.1"
                     /translation="MNDFRRSLRISDQKFGWVAVVVMAVLAVYAIVFVLERMLFLDAP
                     FMLSNIINSGSPRIMEHRYGSVITQIWPWMGMKAGLPLIWLMHLYNGAFNVFYLVVVC
                     LLVFRWRAYGAAVLMALYYTAMSDQAFFWTNNEIHQAVAWFFVWYGFYLYQRKRAGLR
                     RKRFIWFGMTATIAAVTHPLMVPVLAFTWIFLLMTEGRQTGDGKVRVGLTMMIVMACC
                     IRIWFSLRAGWYDRDKVETVKTNLLSGITEVFDKQLAAQLLHQYSHGLIVGAVLGLAG
                     LFLLMVRRKYLLSLMYLLVIVSYHLLFLSAFDTWVDFYSQSEMMPLTIIYGLPVVRFL
                     PEYIGSRLLFSITVMVFLIRLIMIPVASVPFTKRVSNTMSLIREMKEKNITKALIPDN
                     KDLESIYIMTWGLPIETVLASAISGDRQRTVKPLPADKLEEAVQTPSDQFLDCFRIIP
                     INTMNPYYFNLDSVGRYEVVRQ"
     gene            complement(9805..10191)
                     /locus_tag="PLG24_01735"
     CDS             complement(9805..10191)
                     /locus_tag="PLG24_01735"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HMZ23631.1"
                     /translation="MEISEYIAQRSSGEKAICELLATTIDKCLRQSQSKIWHGHPVWF
                     LDGNPIVGFSSQKAGIRLMFWSGADFEEDGLNILGKKFKDASIFYNDVADVSIKDLER
                     WLTKAEAIQWDYKNLVKRKGRLEKLR"
     gene            complement(10250..10687)
                     /locus_tag="PLG24_01740"
     CDS             complement(10250..10687)
                     /locus_tag="PLG24_01740"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ23632.1"
                     /translation="MASINPYIHFNGNAEEAFTFYKSVFGGEFATLVRFKDLNFEGSI
                     STEKEAEKIMHIALPIGEHDLLMGSDTPEILGRHNEHENRSKISVSAESKQEAERIFY
                     ALSEGGQVEMPIEDSPWGTYFGMFRDRFGIEWMVEYNPQVFVR"
     gene            complement(10712..11140)
                     /locus_tag="PLG24_01745"
     CDS             complement(10712..11140)
                     /locus_tag="PLG24_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23633.1"
                     /translation="MTKIAMMPVLWFHVSDGETDPVTNYCNCLFWISLNIEAGIPLGG
                     TAEGMNEMWDLSLFEHRILSGTVVRKRHKFKDSFACRIFFRDQQEIDPRRSYHTTAGA
                     ESRCGLFFDKFSLPWKAIPQGMGISQAGSMHPVHCQTQQE"
     gene            complement(11157..11567)
                     /locus_tag="PLG24_01750"
     CDS             complement(11157..11567)
                     /locus_tag="PLG24_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23634.1"
                     /translation="MTKQIFINLAVNDLSQSMDFYTNLGFTNNPLFSDDTAKCMVWSD
                     SIFVMIMTHEKFESFATKPIADTKNTLAGLFSLSVESLEEMDRIIEAGLKAGGVEPNE
                     MRDYGFMQQRTIEDFDGHTWEIFYMDMSKFPQGA"
     gene            complement(11595..11993)
                     /locus_tag="PLG24_01755"
     CDS             complement(11595..11993)
                     /locus_tag="PLG24_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23635.1"
                     /translation="MKHKILNLLLIITSLLGYLEWSGDSRSFLFQAEGEILYKLFTDP
                     ASTIHPFILLPLLGQLLLLVTLFRKRPGKVLTYIGISGLGLLLGFMFVVGIISLNIRI
                     IASTLPFLLVALYTIYCLRGTKSLANSMYL"
     gene            complement(11995..12408)
                     /locus_tag="PLG24_01760"
     CDS             complement(11995..12408)
                     /locus_tag="PLG24_01760"
                     /inference="COORDINATES: protein motif:HMM:NF039673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6157 family protein"
                     /protein_id="HMZ23636.1"
                     /translation="MKIYTTNYFNTFILVAADCPVLTGEVPPYRGDTKSAANIQYELI
                     NNNPYRYTSDDVLFQVFALKNNIEEKDRESAREQFFAKGQACFRTSPLTKRYGWGVHF
                     DGEGRMAIYSFSSAAYEMFALDTSLKVIRAMKSSR"
     gene            complement(12436..12894)
                     /locus_tag="PLG24_01765"
     CDS             complement(12436..12894)
                     /locus_tag="PLG24_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HMZ23637.1"
                     /translation="MRPLEKTIQEILDNIPTERKEAFHRLHHVIVDNLPEGFEVGISY
                     GMLGYVVPHSIYPAGYHCKKEEPLPFASLASQKNSINFYHMGIYADPGLLEWFVTEYP
                     KYCKSKLDMGKSCIRFKKPDDIPFGLIGALMRKMTVAQWINTYETAFRKK"
     gene            complement(12905..13408)
                     /locus_tag="PLG24_01770"
     CDS             complement(12905..13408)
                     /locus_tag="PLG24_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ23638.1"
                     /translation="MRKGILIIAGIVTSFVLGYFLNFAYALTSGNKEEQSKKVEVTDN
                     PKLGAFSVSLSVRDLKASKEFYEKMGFSILGGSMEKNYLIMKNERALIGLFQGMFQGN
                     ILTFNPGWDENGQNVKKFDDIRVIQRRLKQSGLTLTSEAEEGSTGPASLTLTDPDGNI
                     ILIDQHR"
BASE COUNT         3510 a         3224 c         2854 g         3986 t
ORIGIN      
        1 ggtatcgcaa tattcatttt acctttatct gtcaggctct cacttattcg ccagctgacc
       61 gcatgcagcg tcaatgtcct taccgcgact gcggcgtacg gtgacattga catgatgctg
      121 gttaagcgtc cggacaaatt tgtccaattt gtcttctgtg gttttatgga aggccagacc
      181 ctccaccgtg ttgtattcaa tgatgttgac acggaccggg aagtccctgg tcaatgccgc
      241 cagctgaaca gcatcctctt cattatcgtt gaaattctga agggccagat attcgtaggt
      301 gatgcggttc ctggtttttc gatagaaata gcgcagtgca tccatcagga tgtctatgcc
      361 gttttggtca ttgataggca tgatttcgtt cctcttgcca tcattggcgg catgaagact
      421 caatgcgaga ttagtcttta caccatcatc tgcaagcctg cggatcatct tggcaatacc
      481 cgccgtactg atggttatcc gggaaggcga catgcccagc ccttttggag atgtgatgat
      541 atcgatgctt ctgagcgtgt tggagtagtt gagcaatggc tctcccatcc ccatatatac
      601 gatattggtc agcggatggc caaagtgttc caatgcctgc cggttgaccc gaaccacctg
      661 atcgtaaatc tcgggaaagt ccagattccg tatgcgcccc atcattcccg tagcacagaa
      721 tttgcatgca aggctacagc ctacctggga ggaaacacag gcagtaaaac gtttttcgtc
      781 cggaaccgga atgagtactg actcaatcat atgaccgtca tgcaacttga atcttgactt
      841 gatggtacca tcccggcttg tctgcacaaa atcctcttca atcggcctga atacgaattc
      901 tgctttcccc ttttcccgca aagcaagggg aagattcgtc atttcatcga aactcaaggc
      961 ctgtttctgc catatccact ccgatatttg gccggcacgg aaacgcggtt caccttgctg
     1021 tttgagccat tcttccagtt gggaaggaga gataagccta atgtcgttgg tcatagattc
     1081 tgtggattaa atgcaaagat aaaacaatga tggtattgcg cctgcaatgt ttaacaagct
     1141 aactattgca accgatattt acttataaac cttaatccca aaaatgagct cccaggttct
     1201 tgtaaccact ccctcaaatc agttgaattg tacgtgagat tcgaatttcg gattttccct
     1261 gaacatagcc tgacaaaccc tgtcaatgtc ttatgccaga tatctttcac gttatttatt
     1321 acccggaaaa aatactttta ttcatttact gctatcagaa aattgcatct gagacagacc
     1381 cttgttgtta ccattgttgc cgaatcatca cgctcaggat acatgcctca aaaccattcc
     1441 tgtatttttc tacacttaat attccattcc ttaatatcta catggtcaat taaagaaggt
     1501 tacttactat ttatcccctt tttactcccg gaaatattgt tatcagattt ttatacgaac
     1561 tctgtttccc atccattgca ggatgtcctc aaaaacctcg agtcggatat cctcattcaa
     1621 tatttcgtga cgcattccag aatacaattt tatggtagtg tctgaaaatc ctgccttctc
     1681 caggtgtctt gccactttta ggacgccttt gccaaagtct cctatgggat catccgaccc
     1741 gcctacaaat aaaatcggct gatctttagg caggttacct gaccactcgg gactcgtggc
     1801 gcggacatgg acagtaagaa ggcccaggaa gccattgacg gtaaatggaa caccacactg
     1861 tggatcctgt tcgaaagcat tccggttgtc catactgaca ctcagccagc ttgtggcacc
     1921 ggcattgacc tcgtgcctga accgggcatt attgatcgtc ccgaagatgt tgttgaggaa
     1981 ggaagggcgg cttttcggtg aaagcacatt gaacaagtgt agccctgcct tgatgagaat
     2041 ggcgccgggt gttctgcttc cggtaccaac taagatgatg ccttgaaaat cggacttttc
     2101 ctgtctgaga aaagtcctca ggataaatga gcccatggaa tgaccaagaa caaagtgcgg
     2161 tagtcccggg aattggcctt ccagcaaaga tttcatggca cgaacatcct cgagcaaaag
     2221 ctgtgcaggt ttgttttttc tgaaaaatcc catctcagac atattccggg cagtctttcc
     2281 atggccgcga tggtcatagg tcaataccac aaaaccccgc tccgccatat atcgcgcgaa
     2341 ttcgtcataa cgtccgctgt gctcctgcat gccgtggagt accagcaagg tggctcccgg
     2401 cacaatgtcc ttcctttgat aaatagtgta aaagagggca taatcattgc ccgcatggtc
     2461 attgcgttgc agaaactcgt gttgggtatc gaccatcttt gctgttttat ttactgatac
     2521 aaaatatttt aaaaattgtt tgccggcgac aatatggttt aactactcca tcttttagtt
     2581 ggcttgcagt ataaatctca ttctacagcc atgtttatca cttccggcag aagggtgtag
     2641 tttgtgctat tgataccagg gcattaagga gcgttgaggg cctccgtaac caccttcatt
     2701 tctcaggatc aggacctgtc gacaggcacg gcggttaaat ggtcccttct gtcgttttgc
     2761 ccattgggcc ggttgaataa aaccgattga tactgccaat gatcaaccgg gcatctacgg
     2821 cgatcaaaga aaacaaaatt caactaccct actgggctat atatagggat gaagggggat
     2881 gaatacttcc ccggcgggcc ctgtcagcct ttggtgtatt ttttcaattc aatctcagat
     2941 tcgccatcct ttactctgag caggtcattg ctatattcga tgatatcagg acgaacttcg
     3001 tccattctgc cattgatttt ataaaacaga aacaatttgt tttccccgat attccaataa
     3061 tatccatcca aaggaacagc tgttcggtcc aatttcaaaa tgcttttatc gctattgaac
     3121 aagtaatagg catcctgtct aaatgccgtg gaagccttcc ccttaatact tacgatctgc
     3181 cataatccgg ggaagttgtg ttctgtaatt tctgagtcac ttttgacttt tcctgcattc
     3241 aatccggatt tgttggcttc aatattgatg ccgttagctg tatcccggcc gccggaagat
     3301 tgtaaaagtc ccaggtcggc caattcatct attccctttt gattggttga aataaccact
     3361 ttcaggaaat tgaccggaaa tgaccttgta gtctgcatgg gctttgtcat agccatcatc
     3421 aatgtcaaag tatctccttt ccagcttcca tcaatactga attcagtttt tgccacggta
     3481 tccgttgcgg tagccttgat gccatcgccg attatttcac catgaatcag ataggtggat
     3541 ctgcggtcaa aaatcgtacc ttttatggta gttttatccg gctgcgaaag tgacatggac
     3601 aggctctttc ctctaatatg gccgacatag gttccggtat aatctctttt gtccgttttt
     3661 tggcacgagg tcgcaaatac caggcataac cccagtaaaa tcagcatttt tctatgtatc
     3721 aatatctatc agtttttgtt attcaatgat cataaaacgt gcgacagtcc ggttatgtcc
     3781 atcgatgata tgaagatggt attgtcctgc tgtccggctc cctatattca ggctatatct
     3841 acctgaggcg tccttgtcca taaccctttt caatatttta tggccggtgt ctgagatgac
     3901 ttccgctgta aattttccgt tatttgtgcc ggagatatag ttaaaactga gcacatcctt
     3961 caccggattg ggcataatgg tgattgcttc cagggctccg atggtatttg ttgaaaggat
     4021 ggaagaaaga tcaatccccg aagtcgatcc gtttaatggt tttgcaatgg tggccagtgt
     4081 tgcaatgcct gtcttaacaa ccttggccat aaaatcaaag ttgatggtct ctattttgtc
     4141 tgacagctga tggtagtttg gattgcgggt ttcggcgcca tcggtaatca tcagggccgg
     4201 atagccttta tcccagaagg gtgcatggtc gcttctgcgc aggataggag ctattgtacc
     4261 attgcccggc aatggcaatt cgatcacctt aagtccggga acatatgtcc tggcagcttt
     4321 ggtaaaggcg gccataaggc caaatgaagt ctccgctccg gtattgacga tgaaattggc
     4381 acgtttctga tcgtttgtga tctccacaac ctgattggga aagagcagtt caaatcccgg
     4441 cggaatagcc tgactgtttg gctcatcgga ataataaccc aacatctcat aattgatcag
     4501 accgatgatt tgctggtccg gctcaatccc cttcgatacg taattgatac ttccaataag
     4561 tcccaattct tccaagttaa aggaggtaaa ctgaacatta ttttcaaaca aataatcact
     4621 catgacgcgg atggcttcga ggacacccac tacgccggta ccgttatcat cagctcccgg
     4681 ggcaaactcg gcagaatcaa agtggccatt gatcaggcac aatttattgt tgttgagccc
     4741 ttccagtctg cctatcagat tggaaaaaga caagccgcta aatgtaaaat cctgatgatg
     4801 gatggacagt ccatggtcga tcatgtactg attgatataa ttgccgacat aaatcaggtg
     4861 attgggattg gacttataat gtctcacacc gactatatcg ccgagcatct tttgcaggtt
     4921 ggagatatct accttatcca ccatttcctg aatagatggt gtatgccggt cataagccag
     4981 caaacgaaga gaaacattcc tgaggtcagt ccctgaagga tacgaatata ttattgactt
     5041 ggcttctcca ggaaggatac ctgatccaac ctcgccggtt actccggatg ccgtgaggaa
     5101 attaccggat cctgtttttt gttgcaaaac tactgtagct gtgtcggatt ccacatccat
     5161 caggtcatag gtaattgtta tgcggtggtt ggccgcatcg tggctcacat gcaatggtac
     5221 tgttacaggg ggtgtgttct gcccatataa aacagaacaa ccagctaaca acaatcctcc
     5281 aatgattctt atcatggttt taaattttta taactaaggt aatacatttt cgaaaaatag
     5341 ccggtttatt tacctttcct ttgtaccact acgcattcct gtttctatat ctttagccaa
     5401 ttttcccgcc ggattgcata taccagttca tcctgatacg ctccattttt ataaataacc
     5461 ttatcaaaac gaccttccag cttaaaatgg ttcttttcca aaaccttctg tgatgcgata
     5521 tttgtaccaa agggcctggc aaatattctg tcaatttcaa aggttctgaa tccaaaatca
     5581 accatttgcc tgacagcatt gcttatgatt cctctgcccc agaaaggctc gccaagccag
     5641 tatccgagtt cggcattctt taaaaagata tcgctttggg gatgaattcc aatgcctcct
     5701 gctgcctcac catcgatttc aatagcaaag atatgatttg gcctgccggc cattgcaaat
     5761 tcgatgaata tcttacctgc atcttccgta tatggaaagg ggaaatgatt ggtcaggttc
     5821 ctggagatgt tgaaattatt ggcatacttc accaaactgg tcaaatcagc aaggacccag
     5881 ggcctaatga taaagtcagc cataatattc tatttttggt atcagattag tgcacaccat
     5941 ccacaatatc tcttcatcgc taccggccga aggactaaat accgatttcc aacttcccaa
     6001 tacggctaat ggctgcctgc tgatcccaaa ttttcctaaa cccacctctt aagagtccag
     6061 cttgagtccc agttctttca actgaccgcc gtcgatggtc gcaggcgcat caatcatcat
     6121 gtcccgcccc tggttgttct tcggaaaggc gataaagtca cggatactgc tgctaccgct
     6181 catgaccgcg caaagcctgt cgaaaccaaa tgcgatgccg ccatgtgggg gtgctccgta
     6241 ttcaaaggca ttcatcagga agccaaattg gcgctccgcc tcttccggac taaagcccag
     6301 caaacggaaa ttcttcgcct gcaactccct gtcgaagatc cgtatagacc cgccaccgat
     6361 ctccgaaccg ttgatgacca ggtcgtaagc atcagcacgc aggctattga gcacctcctt
     6421 atcatcggac tgcatctttt caaggtcagc catcttgggg gatgtgaatg gatggtgcat
     6481 ggcgtagaat cgctgtgtct cttcatccca ctccaataac ggaaaatcca cgacccacaa
     6541 cggcttgaag tcaccttccc gcatcaggcc caggcgacgg cccatctcca aacgcagttc
     6601 attgagttgc tttcgggtct tttccaattc gcccgacagg atcaacaaaa gatcgcctgt
     6661 ctctgcccca cattccttgc ctatgcgcag caggtcttct tcggaataaa acttgcccac
     6721 agaagacttg atgccgtcgg cgccatattt gacatatacc aggcctttgg cgccaatctg
     6781 tggtcgttgt acccattctg tcagctcctt caaatccttg ttggagaagg cggactcaca
     6841 gcccttggcg cagatgccta tcaccgtttc tgcactgtca aacacaccga agtcacgacc
     6901 acttaccgcc tctttcagat agttaaattc cataccgaaa cgcatatcgg gtttgtcgga
     6961 accaaaacgg tgcattgcct catcataagt catccgcggg aatgtgccca ggtctatctg
     7021 gagtacactt tggaagaggt gccgggtaag gccttcaaaa gtgttcagga tttcctcctg
     7081 tgtgacaaag gacatctcac agtccacctg tgtaaattca ggttggcgat ccgccctcag
     7141 atcttcatcg cgaaaacact tgacaatctg aaaatattta tccagtccgc tgaccatcaa
     7201 caattgcttg aaggtctgtg gcgactgtgg cagcgcatag aatgagcccg ggtgcattct
     7261 ggacggaacc acgaaatcgc gggcaccctc aggtgttgat tttatcagga atggtgtctc
     7321 gacttcaata aagccttcat cggccagata ttttctggtt tcaatagcaa gattcgacct
     7381 gaatatcaag ttgttcttga cagggtcgcg tctgatatcg aggtagcggt acttcattct
     7441 aaggtcgtct ccaccatctg tattctcttc gatggtaaat ggtggaatcc tggatgcatt
     7501 gaggatatgc aatgtatcga catctatctc gatctcaccg gttgcacggt ttggattctt
     7561 gttggagcgt tcacgtacct taccggccac ccggatgaca tattcgcggc cgagtttctg
     7621 ggctttctcc ctgatttccg gattcgagtc agcgtcaaat acaagctggg ttatgccata
     7681 cctgtcccga aggtccaaaa aagtcagacc gccgaagtct ctgcttacgc tgacccagcc
     7741 acttagagtc acattatttc cggcatccga aagtcttaat tcaccacaat tgtgcgtcct
     7801 gtacatagtt tttcttttga aggcgcaaaa ttacgtttta tagtgctgta aaaaaattat
     7861 aagaaaagga aatttgagat tgattgggtg atttcgcctt ttcgattctt acacgggaat
     7921 ttattgtgtc tttcttccat gattgtcaga atcccggagg aaaagatttt tgtgccttag
     7981 ggacttcggt caccaattac aatcctcctt caccggcccg catctcagct atcttccggc
     8041 ttatggcaaa gagaatcaac ttcaataaaa taactatctt acacttcaaa tggcattaat
     8101 tgcatgaatg atttccgaag atcgttgcgc atatctgacc agaaattcgg ctgggtggcc
     8161 gttgttgtaa tggctgtctt ggcggtatat gccattgttt ttgtgttgga aaggatgttg
     8221 tttctcgatg cgccatttat gctatccaac atcatcaaca gcgggtctcc ccggattatg
     8281 gagcaccgat acggctcggt catcacacag atatggccat ggatgggcat gaaggccggc
     8341 ctgcctttga tctggctcat gcacctgtac aatggggcgt tcaatgtgtt ctaccttgtc
     8401 gtagtatgcc ttcttgtttt ccgctggcgc gcctatggcg ccgccgtctt gatggcactt
     8461 tactatacag ccatgtccga tcaggctttt ttctggacca acaatgagat tcatcaggcc
     8521 gttgcctggt tttttgtatg gtatggcttt tatttgtatc agcggaagag agcaggactt
     8581 cgccggaaaa gattcatctg gtttgggatg accgcaacaa ttgcggccgt tacgcatcca
     8641 ctaatggtgc ccgtgcttgc ttttacctgg attttcttgt tgatgacgga aggaaggcag
     8701 acaggcgatg gtaaggtccg cgtggggctg acgatgatga tagtcatggc atgttgcatt
     8761 cgtatttggt ttagtctgcg tgcaggatgg tatgaccggg acaaggtaga gaccgtgaag
     8821 accaacctgc tttcgggcat aactgaagtc ttcgacaagc agttggctgc tcaattattg
     8881 catcaatata gccatggcct gattgtagga gccgttctgg gcctcgccgg cctattcctg
     8941 ttgatggttc gaaggaaata cttactttct ttgatgtatt tgttggtcat cgtgtcttac
     9001 cacttgttgt ttttgtctgc ctttgacacc tgggtggact tctattcgca gagtgagatg
     9061 atgcctctga ccattattta cggattgccg gtggttcgct ttttgccgga atacattgga
     9121 tcgaggctat tgttcagtat tacggttatg gtctttctga taagattgat tatgattcct
     9181 gttgcatctg ttccttttac gaagcgtgtg tcgaatacga tgtcgcttat tcgcgaaatg
     9241 aaggaaaaga atatcaccaa agcattgatc ccggacaata aagatcttga gtcaatctat
     9301 attatgacat ggggcctgcc gatcgaaacc gtcctggcat ccgctatctc cggagaccgc
     9361 caaaggaccg ttaagcccct gcctgccgat aagctggagg aagctgttca aactccctca
     9421 gaccaatttc tggattgttt caggattatt cccatcaaca ccatgaatcc ttactacttt
     9481 aatctggatt ctgtagggcg atatgaagtg gtccggcaat aaactgcatt ctgtctgaaa
     9541 aggaaattta tcgaatcaaa gattccgtcc acatacgccc ggtcagcttg tgcccacaat
     9601 gcatatagtc cgataaacca taggatacct catcctatta atagtggtcc aagcttgaat
     9661 acatcgattg gaaattgatt aaaaaggttg gttcgggtgc cgggcattca ggcccgaaat
     9721 gcttacctta caataataca tagattccat ccgacagcat atacccaccc attaaaaagc
     9781 cccaaatgtt tagtaaattt tcacttatct taatttttct aacctaccct ttctctttac
     9841 taaattttta taatcccatt gaatggcttc tgcctttgtc aaccaacgtt ccagatcttt
     9901 gatgctgaca tcggcaacat cattgtaaaa gatggatgca tccttgaatt tcttcccgag
     9961 gatattcagt ccatcttctt caaaatctgc accactccaa aacatcagac gtattccggc
    10021 tttttgagag ctgaagccaa caatcggatt accatccaga aaccaaacgg gatgcccatg
    10081 ccaaatctta ctttggcttt gcctcagaca tttatcaatc gtcgtagcca gcaactcgca
    10141 tatggctttt tctccggacg acctttgagc tatatattca gatatttcca tcgataacac
    10201 acagttagat atgatatact acctgttcta caattttgat gattggttgt taccggacaa
    10261 aaacttgcgg attgtattcc accatccatt caattccaaa cctatcgcga aacattccga
    10321 agtatgttcc ccacggactg tcttcaatag gcatttccac ctgccctccc tcagaaagtg
    10381 cataaaaaat tctttctgct tcctgcttgc tttcagcgct tacggatatc ttgctccgat
    10441 tttcatgctc gttgtgtctg cccaatattt caggagtatc acttcccatc agcagatcgt
    10501 gctcgccaat cggcaaagcg atgtgcatga ttttttcggc ttctttttct gtgcttatgc
    10561 tgccttcaaa gtttaaatct ttaaaacgca ccaaagttgc aaactcgccc ccaaaaacgg
    10621 atttatagaa agtaaacgct tcttctgcat ttccattgaa gtgaatgtac ggattgatgg
    10681 atgccatatt tttattattt agtttgattg attactcttg ttgggtctga cagtgcacag
    10741 ggtgcattga gcctgcttgt gatataccca tgccttgggg tatagccttc cagggcaggc
    10801 tgaatttatc aaagaatagg ccacaccttg attcagcacc tgctgttgta tgataactcc
    10861 ggcgtggatc tatctcctgc tgatccctga aaaagatcct gcaagcaaat gaatccttga
    10921 atttatgacg tttcctgacc acagtccctg ataaaattct atgttcaaac aggctaaggt
    10981 cccacatttc gttcatccct tccgctgttc cacctaatgg aatacccgcc tctatgttca
    11041 aactaatcca gaaaaggcaa ttacaatagt ttgtcacagg atctgtttct ccatcagaga
    11101 catgaaacca taatacaggc atcattgcaa tcttcgtcat atatcaatgc ttttacttag
    11161 gctccctgag ggaacttgct catgtccatg tagaatattt cccacgtatg tccgtcaaag
    11221 tcctcgattg ttcgttgttg catgaagcca tagtccctca tctcattagg ctccacacct
    11281 ccggctttca atccggcttc aataatccgg tccatttcct ccaggctttc aaccgaaaga
    11341 gaaaacaatc ctgctaaagt gttcttggta tcggcaatag gtttggtagc aaaggattca
    11401 aatttttcat gggtcataat catgacgaaa atagaatcgc tccacaccat gcacttagcc
    11461 gtatcgtccg aaaatagtgg gttattggta aatcccagat tcgtgtaaaa gtccattgat
    11521 tgggacaagt cattaactgc cagattgatg aagatttgtt tagtcattat ttttaatttc
    11581 aaggttattg attcttataa gtacatgctg ttcgccaggc tctttgttcc cctgagacaa
    11641 taaatagtat atagcgctac caatagaaac ggtaacgttg aggctattat cctgatattc
    11701 agacttatga ttccaacaac aaacataaag ccaagcaaca gccccagtcc tgatatgccg
    11761 atataagtaa gcaccttgcc cggccgcttc cggaataagg tgacgagcaa caaaagttga
    11821 cccagcagtg gcagtagaat gaatgggtgg atggtggaag caggatctgt aaataattta
    11881 tagaggatct ctccttctgc ttgaaaaagg aaagaacggc tgtctccact ccattccaga
    11941 tagcccagta aggaagtaat aatcagtagt aaattaagaa tcttatgttt catattatct
    12001 gcttgatttc atggccctga tgacctttag agacgtatcc agagcgaaca tttcataagc
    12061 tgcactgctg aaactatata tcgccatcct cccctcacca tcaaagtgga caccccaacc
    12121 atagcgcttg gtcaggggag atgtccggaa acatgcctgt ccctttgcaa aaaattgctc
    12181 cctcgccgac tcacgatctt tttcttctat attattcttc aaagcaaata cctgaaacag
    12241 gacatcatct gatgtatacc tgtaggggtt gttgttgatt aattcatact ggatattggc
    12301 tgcgctttta gtgtcacccc tgtatggtgg aacctcgccc gtcaggacag gacaatccgc
    12361 tgcaacgagg atgaaggtgt tgaagtaatt cgtcgtgtat atcttcataa aattcaagat
    12421 ttcgtcaaga gggaatcact ttttcctgaa tgctgtttca taagtattaa tccattgtgc
    12481 tacggtcatc ttacgcatga gggcaccgat taatccgaaa gggatatcgt caggtttctt
    12541 aaagcgaata cagcttttgc ccatatccaa tttactcttg caatatttcg gatattctgt
    12601 cacaaaccac tctagcagtc cgggatcggc atagatgccc atgtgataaa aattgatcga
    12661 atttttctgt gaggccaatg aggcaaaagg gagcggttct tcttttttgc agtgatatcc
    12721 ggcaggatat atgctgtggg gcaccacata gccaagcatt ccgtagctta tccctacttc
    12781 aaatccttcg ggcaggttat caacgatgac atggtgcagt ctatgaaatg cctctttcct
    12841 ttctgtggga atattatcca gaatttcctg tattgtcttt tcaagtggcc tcatgagtat
    12901 attattatcg gtgttgatcg atcagaatta tatttccatc aggatcggtc aatgtaaggc
    12961 ttgccggacc cgtggatccc tcttcggctt cgcttgtcaa tgtcaaaccg gattgtttta
    13021 accgcctttg aataaccctg atatcatcaa acttctttac attctgtcca ttttcatccc
    13081 acccggggtt gaatgtaagg atgttgccct ggaacatacc ctgaaaaagg cctatcaacg
    13141 cccgttcatt tttcattatc aggtaattct tctccatgct tccacctaga atgctgaaac
    13201 ccattttttc gtaaaactct ttggaagctt tcaagtccct gacactaagg ctcactgaga
    13261 aagctccgag ttttggattg tctgtcactt caaccttttt cgactgctcc tccttattac
    13321 ctgaagtcag agcatacgcg aaattcagaa aataacccag aacgaaggag gtaacaattc
    13381 cggcaatgat taaaattcct ttccgcatga tatttatcaa ttttttgaat gaaaaaatta
    13441 aagaaaaaag taaaatctcc tggcgagact aatcataaac tgctcagggc attgttttaa
    13501 ataaccggca taaatttacc agaatagtat taaaaactat aaataatttc gacgaaggaa
    13561 agcttacaaa tcat
//
LOCUS       DAOQQN010000026        13506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1535_length_13506_cov_5.589845, whole genome shotgun sequence.
ACCESSION   DAOQQN010000026 DAOQQN010000000
VERSION     DAOQQN010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13506
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1535_length_13506_cov_5.589845"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2575
                     /locus_tag="PLG24_01775"
     CDS             <1..2575
                     /locus_tag="PLG24_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HMZ23639.1"
                     /translation="NSAERRAAYLCDITYGTNNEFGFDYLRDNMVTSPDELVQRKHHY
                     AMVDEVDSVLIDDARTPLIISGPIPQGSDEQEYIDLKPRVERLVDAQRKLATGFLVEA
                     KKLISENISGNAEGEGGLALLRAYRALPKTRALIKYLSEEGVKVVLQKTENYYMQEQS
                     KNMHIADEPLLFTIDEKNRDVELTEKGAEYLAQGMEDKDFFVLPDIGTEIHSLEHDSS
                     LTAAQKAEEKEKLIQDYTAKSKRLHSINQLLKAYALFEKDVDYIIVEGEVKIVDEQTG
                     RVMEGRRYSDGLHQALEAKENVKVGEQTQTYATVTLQNYFRMYHKLAGMTGTAETEAS
                     EFWQIYKLDVSVIPTNKPIVRKDMEDLVFKTAREKYNAVIEEISTLTKAGRPVLVGTT
                     SVEISEMLSKMLRLRGIEHNVLNAKQHAREADIVAEAGHPGRVTIATNMAGRGTDIKI
                     SDQVKAAGGLAIIGTERHDSRRVDRQLRGRAGRQGDPGSSQFYVSLEDNLMRLFQSER
                     IAKAMDRMGHKEGDVIQHSMITKSIERAQKRVEENNFGIRKRLLEYDDVMNIQREAIY
                     KKRNNALFGDRLSVDLSAMFSSATEDVVLRGRQLHSFEEFRELSIRELGFDPADLGEG
                     FTAMNEDQAIESFQVAFMKYYNTKNQRISEVILPIIKNIKEREGDRFKRIALPFSDGS
                     AHPLPVSAGMNEAIESDGRSIASDLEKTITLSIIDDKWKEHLRNMDDLKDAVQAASFE
                     QKDPLVIYKMEAFTLFENLVGKINHEVTAYLAKGNMAIAMEPEQVREARVQKTDFSKA
                     RMHKDNSEGYDDDPMREGPPKSTVEKPVTYIRSEKKVGRNDPCPCGSGKKYKACHGSH
                     M"
     gene            2633..3334
                     /locus_tag="PLG24_01780"
     CDS             2633..3334
                     /locus_tag="PLG24_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23640.1"
                     /translation="MTKLFVLISKQRHTFRRQRFFLAAIWMLEAFLCFSQQSDSMRIY
                     TQVDHMPQLVSCASGYGNMYKLDRCTVASLKAWMLANMQYPATALESGREERYSFRAL
                     VDTTGQIKEIKPAQDLSSECGNEANRLTKLMLQSGEKWTSGRQGTRKVPVWIEFCIEF
                     NAKDWQNELTRRANPQAFVSRDSLALKPVKPEMPKAKSKVKPKSNSKAGNKAKKNSSS
                     KSKYSKKKTSTKKKR"
     gene            complement(3362..3943)
                     /locus_tag="PLG24_01785"
     CDS             complement(3362..3943)
                     /locus_tag="PLG24_01785"
                     /inference="COORDINATES: protein motif:HMM:NF025167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HMZ23641.1"
                     /translation="MNDINYFEEGWFHIMSLDALDHQLFIISLAALFLLKDWRRVLVL
                     VTAFTIGHSLTLALSAYDIIRFSTAWVEFLIPITIVITALYDLTYTNLSKIEKRNINF
                     NYIIALSFGLIHGMGFANSIRMMLASDQRIGMPLLSFNLGLEAGQIVLILLILLAAQL
                     IVVNMKIPRKYWIILLSVIPLVLSFKMALERIP"
     gene            complement(4074..4943)
                     /locus_tag="PLG24_01790"
     CDS             complement(4074..4943)
                     /locus_tag="PLG24_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMZ23642.1"
                     /translation="MKRAYLILHIAVFLAGFTGIFGKLISLSQFPLVWFRVLFSVLIL
                     FLGLRLLKIRIGGGKAQIFKIATVGQLLIIHWVFFYGSIKYANVSVAAICYSLTSFFT
                     AIFAPIINRKKLQFGQLALSSITLSGIGLIFHFDTTFHTGIILGVISSAFAALYTITN
                     EKLVRIHDSRLINLYQLSGGVITLGLFLPFYYHFYPEQAFLPGGMDVVYLILLAAFCT
                     VALYLLFAEALKHLSAFSVNMAFNLEPIYAIGLAFLIFREGHEVNESFYWGLALVVLS
                     VFLQTLMNFYGRR"
     gene            complement(4950..5537)
                     /gene="nadD"
                     /locus_tag="PLG24_01795"
     CDS             complement(4950..5537)
                     /gene="nadD"
                     /locus_tag="PLG24_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HMZ23643.1"
                     /translation="MNHRIGLFFGSFNPVHVGHMIIANHLVNETDLDQVWMVVSPQNP
                     FKEQKNLASDRFRYHLVYLAIGDNPKIMASDIEFGLPRPSYTIDTLAYLHEKYPEKEF
                     CLIMGGDNVSTLNKWKNSELLLERYRIYVYMRPGYDLGPLGQHANIIPLEAPLLDISA
                     TYIRQLLVSGKSVRYLLPEAVRMELEKSNPYRDLQ"
     gene            complement(5546..6670)
                     /gene="dnaN"
                     /locus_tag="PLG24_01800"
     CDS             complement(5546..6670)
                     /gene="dnaN"
                     /locus_tag="PLG24_01800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HMZ23644.1"
                     /translation="MILRISSGQLYKMLQVGQNAISSKPPYPILADFLFSMDGDKLSI
                     TSTDLEFTIISETTITPSDTGQFAFPAEILVSTLKEMPEQPIELIFEEADNMLKMISA
                     YGNYRSSSNSIEDYPEIPEIRTESQINIPASRFLKAISTCVFATATDEMKANMLGIAI
                     NIQPDAVIVAATDAHKLVKYTIFHDNGENESELVIPKKAVSVLRHIIEDDENLQINYD
                     RNHIFFEYKNYKVFCKLINQKFPNYNSVIPINNDKDVFINRQEWLQSLKRITLYGNRT
                     TYQTSFHVEENEIKINTIDPDFSNEASEVIRCQYTGEPLDLSYNAKFLIDSLSNLDAE
                     EVKLSISRPNRAAILTPKEDKPGENILMLVMPVMTKRLTE"
     gene            complement(7383..8363)
                     /locus_tag="PLG24_01805"
     CDS             complement(7383..8363)
                     /locus_tag="PLG24_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HMZ23645.1"
                     /translation="MSSTEDLAIGIDIGGTNTKFGIVNRNGKVFHRGEIKTRKHAEVA
                     PFIDELYLSILPMIEYAGGPARIRGIGVGAPNGNFYNGTIEYAPNLSWKGVIPLAKMF
                     REKFDMDVILTNDANAAALGEMSYGAARGMRDFIVITLGTGVGSGIVANGHMIYGHDG
                     FAGELGHTMIIPEGRYHPGTGLYGSLESYASATGVARTAVEFLNSRPTVESLLRQYAN
                     EEITSKVVFDCARKGDSLSKEVYSYTGKILGMALANFAMATSPEAIILFGGLCNAGDM
                     LFGPVTDSFEKHLLPLFRNKIKILPSELPDDDAAILGASSLVWDKERGGN"
     gene            complement(8389..9867)
                     /locus_tag="PLG24_01810"
     CDS             complement(8389..9867)
                     /locus_tag="PLG24_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ23646.1"
                     /translation="MSNNQSGTIWSQFLTLITVFFFWGFVAASNDILIPVFKENLHLS
                     QTQSQMVSFAFYTAYTVGSLIYFFISKMRGGDILNKIGYKNGIAIGLVISALGTLMFI
                     PAANTHSFNLFISGLFIVGLGFSLQQTAANPLAIVMGDPKLGSQRLSMAGGVNNFGTT
                     IGPLLVNFAIFGSIYAATAQADITSIKIPYLILGAAFILVALLIKFSKVPNEIDLDQM
                     AENEAEGDSSKVRHLSGPMKYPQLSLGMLAIFVYVGVEVSTASNLPEFMKQHIAGPDG
                     GVFPTDKIAPYISLYWASLMMGRWTAASEAFDLSMSMKRMLRYFMPYIAFGVFLLVNK
                     IANHDITPFYFYVFIIAAMVAGNIMSKGNPARQLLIYSCLGIIALIAGMLADGWVSTF
                     AFISVGLFCSTLWPCIFTLAIAGLGKHTNEGSSLLIMMIMGGAFVSLFQGYLSDHVTG
                     IQYSYIAGVLCFAYLAFYAIRTKNILKAQGIDYDHGVVSGGH"
     gene            complement(10081..10491)
                     /locus_tag="PLG24_01815"
     CDS             complement(10081..10491)
                     /locus_tag="PLG24_01815"
                     /inference="COORDINATES: protein motif:HMM:NF023125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HMZ23647.1"
                     /translation="MKEELLKIYNSTTYLVPSLDLEIRPGIQNSRLNEVLAHYGVNTF
                     SFITAYNPNGIETLGRLNREAHNRLIVEVSRLGLQYVEGYGRGSNGWKEEISLLILGI
                     SLVESGILSQKFKQKAFVFGQINEAPSIIWSEPD"
     gene            complement(10532..12178)
                     /locus_tag="PLG24_01820"
     CDS             complement(10532..12178)
                     /locus_tag="PLG24_01820"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMZ23648.1"
                     /translation="MIFRSRFAIFLFILALCIITTGCKKTKPDEIHFDTKKNFWEYIE
                     KGNEIYSKKSDLKVFGKAKNYFDSAMLIAEHLKDTMLLAEATYAYGKVFDAWNSNPAL
                     TLAYYKKAEELFAKSDSVEAALYMKFHVAHAYNGVKDSTNCIKQINEILGIIDTLPGS
                     VANEIRFLPEVAMEAANAGDFDLAETILDKYVNKSNLKNDTLTLNHKDHYYYTRAVIQ
                     IQKNISTSSYLDTLEQVFQEARTTGDSMFYASVLSKFYEKTGNFKKSLYYSGLNYDMT
                     FRFINKHEYVNLGQKLAEMELNALEQENQIETKSFIILFVSITALALLSLASIYLNVI
                     IGRSRLKHIRVSKELEESHDKISLLYKELHHRIKNNLHMIFSLLQMQERKATVKATIE
                     NLRAARLRIESIAVLHEEMMRETYLVDFRDFIERMIEIIKDCFSYSHEIKTNLDFNDV
                     EIPLKQSFSLALIINEWITNSIKHANTSGKPLAIYINFTEIDDKIKVEYWDNGTVDIR
                     IQPQTGFGSEIITLLAKQIGKLMITDQSNPYYYAILINKN"
     gene            12746..>13506
                     /locus_tag="PLG24_01825"
     CDS             12746..>13506
                     /locus_tag="PLG24_01825"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HMZ23649.1"
                     /translation="MQPINKYSRTVTQDDTQPAAQAMLYGIGLTEEDLKKAQVGIVST
                     GYEGNTCNMHLNDLAKSVKSGVLQEDLVGLIFNTIGVSDGISNGTAGMRFSLVSRDII
                     ADSIETVVSAQWYDAVVAVAGCDKNMPGTIMAMGRLNRPSLMVFGGTIHSGQWKGQSL
                     NIVSAFEALGKKINHDITVEDFKGIIRHACPGAGACGGMYTANTMASAIEALGMCLPY
                     SSSAPAMSAAKQDECLAVGRAVRNLLEKDIKPSDI"
BASE COUNT         4019 a         2763 c         2873 g         3851 t
ORIGIN      
        1 caattccgca gaaagacgag ctgcctacct gtgtgacatc acttatggta ccaacaacga
       61 atttggtttt gactatctgc gtgataacat ggttacaagt cctgacgaat tggtgcaacg
      121 taagcaccat tacgccatgg ttgacgaagt tgactccgta ttgatcgatg atgcaaggac
      181 gccgctaatc atttccgggc caatcccaca aggttcggac gaacaggaat atatcgacct
      241 gaaaccacgt gtggagcgtt tggtggatgc tcagaggaaa cttgcaacag gcttccttgt
      301 agaggcgaag aaattaatta gtgaaaatat ttctggtaat gctgagggtg aaggtggctt
      361 ggcattatta cgcgcttata gggcacttcc aaagacaaga gcgcttatca agtatttgag
      421 tgaagaaggg gtgaaggttg tcttgcaaaa gacagaaaat tattatatgc aggaacaatc
      481 caagaatatg catattgcag atgagccttt gctttttacg attgacgaaa agaatcgcga
      541 tgtcgaattg actgaaaagg gtgctgaata tcttgctcag ggcatggaag ataaggattt
      601 ctttgtactt cctgatattg gaaccgaaat tcactccctt gagcacgatt catcgttgac
      661 tgctgctcag aaagccgaag aaaaggagaa attgatacag gattataccg ccaaatccaa
      721 acgtcttcac tctattaacc agcttttgaa ggcatatgca ttgttcgaga aagacgtaga
      781 ttatatcata gtggaaggcg aggtaaagat agtcgatgag cagacagggc gtgttatgga
      841 aggacgtcgt tattcagatg ggcttcatca ggcactggaa gccaaggaga acgtcaaggt
      901 gggagaacag acacaaacct atgctacagt aacattacag aattacttca gaatgtatca
      961 taagctggca ggtatgacgg gtactgccga gaccgaagca agtgaatttt ggcagatata
     1021 caaactcgat gtttcagtca ttccgaccaa taagcctatt gtaagaaagg acatggagga
     1081 tctggtgttc aagacagcaa gagaaaagta caatgcggta attgaagaaa tttccacttt
     1141 gaccaaggcg ggacgcccgg ttctggtagg tactacatcg gtggagattt cagagatgtt
     1201 gagtaaaatg ctccgattac ggggtattga acataacgta ctgaacgcca agcagcacgc
     1261 ccgggaagcg gatatcgtgg ctgaagccgg acatcccgga agagttacca tcgccaccaa
     1321 catggcaggt cgtggtacgg acattaagat cagtgatcag gttaaggctg ccggtggttt
     1381 agctattatt ggtaccgaaa gacacgactc acgccgggtt gacagacagt tgagaggtcg
     1441 ggcaggacga cagggagacc ctggttcatc tcagttctat gtctctcttg aagacaacct
     1501 gatgcgtctg ttccaatccg aaagaattgc caaagctatg gacagaatgg gtcataagga
     1561 aggcgatgtg attcaacaca gcatgatcac caaatccatt gaaagagccc agaaaagagt
     1621 agaggaaaac aactttggca tccgtaaacg actcctggaa tatgatgacg tcatgaatat
     1681 ccagcgagag gctatttata agaaaagaaa taatgccctc ttcggagata ggctttctgt
     1741 agacctcagt gccatgtttt cctctgccac tgaggacgta gtactccgtg gccggcagtt
     1801 gcattccttt gaggagttca gggaactatc catccgcgag ctgggctttg accctgcaga
     1861 cctgggtgaa ggttttactg ccatgaatga agaccaggcg attgaaagtt tccaggttgc
     1921 ctttatgaag tattataaca ccaagaacca gaggatcagt gaagtcatcc tgcccatcat
     1981 caagaacatc aaagaacgcg aaggggatcg tttcaagcgg attgccctcc cattctccga
     2041 cggaagtgca catcccctgc cggtttcagc cggaatgaat gaagccattg aatctgatgg
     2101 aagaagtatt gctagtgatt tagaaaaaac cataaccctg tccatcatcg atgataaatg
     2161 gaaggaacat ctgcgtaata tggacgacct gaaggatgcc gtccaggctg cttcgtttga
     2221 gcagaaagac ccactggtga tttataagat ggaagcattt accttgtttg aaaatttggt
     2281 gggtaagatc aatcacgagg tgacagctta tcttgccaaa ggtaatatgg ccatagctat
     2341 ggaaccggaa caggtaaggg aagctcgggt gcaaaaaacg gatttttcaa aagcaaggat
     2401 gcacaaagat aattctgaag gttatgatga cgatcctatg agagaagggc ccccaaaatc
     2461 cactgtcgag aaacccgtga cctatatcag gtcagagaaa aaagtcggac gtaatgatcc
     2521 ttgcccttgc ggaagtggga aaaagtataa agcttgtcac ggatcccata tgtaaattat
     2581 ccgaaaatca gtataattgc aacatctcaa cgataatccg ttgagatgtt ttttgacaaa
     2641 actatttgta ttgatttcaa agcagcgtca tactttccga agacaaaggt tttttctggc
     2701 cgccatttgg atgttggaag cctttctttg tttcagccaa caaagtgatt ccatgaggat
     2761 atacactcaa gtggatcata tgcctcaact ggtttcctgt gcctcaggtt atggcaatat
     2821 gtataagttg gatcgctgta cggtggcatc tcttaaagca tggatgttgg ctaatatgca
     2881 atatccggct actgctttgg aatcaggtag ggaagaaaga tattccttta gggcactggt
     2941 tgacactact gggcagatca aggagattaa gcctgcacag gatttgtcgt cggaatgtgg
     3001 caatgaagct aatcgtctca ccaaattgat gttgcagtcc ggtgaaaaat ggacatcagg
     3061 acgacaaggc acaagaaaag ttccggtgtg gatcgagttt tgtattgaat tcaatgccaa
     3121 agactggcaa aatgaattga cacgcagagc aaatccccag gcttttgtaa gtcgggattc
     3181 cctggcttta aagcctgtaa aacctgagat gcccaaagcc aaaagtaaag tcaagcccaa
     3241 aagcaatagt aaggcgggca ataaagctaa gaaaaattca tcttccaaat caaaatattc
     3301 taagaaaaaa acttccacta aaaagaagcg ttaatccttc cgccgacgat ttacctgcta
     3361 tttagggaat tctttccaag gccattttga aggatagtac cagtggaatt acactcagca
     3421 gaattatcca gtatttccgt ggaattttca tgttgactac aatcaactga gctgctaaaa
     3481 gtataaggag tataagtact atctgcccgg cctcaagccc taaattaaaa cttagtaatg
     3541 gcatcccgat tcgctggtca ctggcaagca tcatacggat actgttggca aaacccatcc
     3601 catggatcag cccaaatgaa agtgcgataa tataattgaa attgatattc cttttttcta
     3661 tcttcgaaag atttgtatag gttaggtcgt aaagagcagt aatgactatg gtaatcggta
     3721 taaggaattc gacccatgca gtactgaagc gaataatatc ataagcactt aaagctaatg
     3781 taagggaatg accaatggta aatgctgtca cgagtaccag aacacgacgc caatccttca
     3841 ataggaaaag tgctgccaac gaaatgatga ataattgatg atcgagtgca tcaagactca
     3901 tgatatggaa ccacccttcc tcaaaataat tgatatcatt catattgtgt ttcttattgg
     3961 atgtaaatta aatcctaatc cattttagtt gaattaatgg tacaagatat acaatttatt
     4021 cgttttgtcg atgtcaactc ttcggaaaaa cgatgaggca ataatcctcc attttatctt
     4081 cttccataaa aattcatcaa ggtctgaaga aacaccgaca gcaccaccag tgcaagaccc
     4141 caatagaatg attcatttac ttcatgcccc tccctgaata tcagaaatgc taatcctatg
     4201 gcataaatag gctcaagatt gaatgccata ttgacggaaa aggccgaaag atgtttcaac
     4261 gcttccgcaa ataaaagata aagagccaca gtacaaaatg cggctaacaa aatcaggtag
     4321 actacatcca tccctcctgg tagaaaggcc tgttccggat aaaaatggta ataaaaaggc
     4381 agaaataagc ctaaggtaat aaccccgcct gaaagctgat acagattgat cagcctactg
     4441 tcgtgaattc tcactagttt ttcattggta atagtataaa gcgcggcgaa tgctgaggat
     4501 atgactccta gaatgattcc tgtatggaag gtggtgtcaa aatgaaaaat caacccgatt
     4561 ccggaaagag tgatgctgct aagtgccaat tggccgaatt gtagtttttt acggttgatg
     4621 atgggcgcaa agattgctgt gaaaaagctt gtcaggctat aacaaattgc agcaacagac
     4681 acattggcgt atttaatgct tccatagaaa aatacccaat ggataatgag caattgaccc
     4741 actgttgcga ttttaaatat ctgggcttta cctcctccta tcctgatctt caataacctc
     4801 aatccaagaa atagaatgag tacggaaaaa aggactcgaa accagaccaa tggaaactga
     4861 ctcagagata tcaacttgcc gaatatacct gtaaatccgg cgagaaatac tgcaatgtgg
     4921 agtataaggt aggccctttt caccttgatc tattgaaggt cgcgataagg attgctcttc
     4981 tccagttcca tcctgactgc ttcggggagc aggtaacgca ctgacttacc gcttacaagc
     5041 agttgacgga tatacgtggc cgaaatgtca agtaatggag cttccagggg tatgatattg
     5101 gcatgttgcc ccaatgggcc caaatcataa ccgggtctca tatagacata tatccggtat
     5161 ctttccagaa gaagctcgct gtttttccat ttgttcaatg tggatacatt gtcgccgccc
     5221 atgataagac agaattcctt ttccggatac ttttcatgca gataggcaag cgtatcgatg
     5281 gtataggaag gcctgggcaa tccgaattct atatcactgg ccataatttt tgggttgtca
     5341 cctatggcaa gatataccag atggtatctg aaccggtcac tggcaaggtt tttttgctct
     5401 ttgaagggat tttgaggcga tacgaccatc caaacctgat ccagatcggt ctcattgact
     5461 aagtgattgg ctataatcat gtgcccgacg tgtactggat tgaaagaacc aaaaaacaat
     5521 ccgatacgat gattcatgtt caggattatt cggttagcct ctttgtcatg accggcatca
     5581 caagcatcaa aatattctcc ccgggcttat cttccttcgg tgtcagaatg gcagcccgat
     5641 taggtcttga tatggagagc ttgacctctt cggcatcaag attgctgagt gaatcaatca
     5701 gaaatttggc gttataggaa aggtccaaag gctctcccgt atattgacat cggatgactt
     5761 cggaagcttc attggaaaaa tcaggatcga tggtgttgat cttgatctcg ttttcttcta
     5821 catggaaact ggtttgatag gttgtccggt taccatacaa cgtaattcgt ttcagggact
     5881 gaagccattc ctgcctgttg atgaatacat ccttatcatt attgatcgga atgacgctgt
     5941 tgtagttggg gaatttttga ttgattaatt tacagaatac cttgtaattt ttgtattcaa
     6001 aaaagatatg gttcctgtca tagttgatct ggagattctc atcatcttcg atgatgtgtc
     6061 ggaggacaga tactgctttt ttgggaatga ctaattcact ttcattctca ccattgtcgt
     6121 ggaaaatagt gtactttacc agcttatgag catctgttgc agctacgata acggcatcag
     6181 gctgaatatt aattgcgata ccgagcatat tggctttcat ttcatccgtt gctgtagcaa
     6241 atacgcaggt acttatcgct ttgaggaaac gactggccgg aatattgatt tgtgattcgg
     6301 tcctgatctc cggaatttcc ggataatctt cgatactgtt actgctcgaa cgatagtttc
     6361 cataggcaga aatcatctta agcatattgt cagcttcctc aaaaatcaat tcaatgggct
     6421 gttcaggcat ttccttcaat gtactgacga gaatttctgc aggaaatgca aattgtccgg
     6481 tgtctgaggg agtgattgtc gtctcagaaa tgatagtaaa ctcaaggtca gtggaagtga
     6541 tactcagctt atcaccatcc attgaaaaca aaaaatctgc aagaatcggg taaggaggct
     6601 tggacgaaat ggcattctgg cctacttgca gcatcttata taattgaccg gacgaaattc
     6661 tcagaatcat atctcgagtt ttattttgtt cagaaaaatt tgaggtatta tacccggggt
     6721 ttctttgaaa gggcgaaaat aacaaaatta accgatattt agatagcctg tgcaatatat
     6781 gaatggcata ccttttctct tcccggattt ttagtcaaag cagataggca gtgtaagcct
     6841 tgttagggta ttggtactat aagtgcgcaa atttctcggc actgactcat tgttctgaag
     6901 atataatgat tcacctgaat taataacgaa atcacccgtt ttcaattgaa ataaaatttc
     6961 tccgctttta gtcggtaatt tgcatgatta cattgattat ttctcgcaat gtaaaatttc
     7021 agggtaccca ctttattggc ctacgtgatt ctatggccag gtattatact ttagattgac
     7081 agaacccgat tgtcataatt tttttccaac ttcctttatc ttcaaacaaa ttaggcataa
     7141 tatggtaata gcgaatagaa ggagtgaagc caatttgaca tccatggtta ttaaaattgg
     7201 caatatgcct tcatttgaat ttagtcgtat tacagactat ccgattgact tttgagatct
     7261 ccggctttag attctttatc ctaaggaaaa ataaattatg gcaaacgtag tgtcctatta
     7321 gacctcagta agcatagcct tccggcagcc ttgttagatt ttgactgaat tggataccta
     7381 cttcaattcc cgcctctttc cttgtcccaa acgagggagc ttgcacccaa tattgcagca
     7441 tcgtcgtccg gcaactcact gggaagaatt ttgatcttat ttctaaacag aggcaaaaga
     7501 tgcttctcga aagagtctgt taccggtccg aaaagcatgt cgcctgcatt gcacaaccca
     7561 ccgaagagta tgattgcttc cggagaggtc gccatagcaa aattagcaag tgccatcccc
     7621 agtatctttc cggtataaga atatacttcc tttgacaagg agtccccttt tcttgcacaa
     7681 tcgaaaacaa ccttgctggt aatttcttca ttggcatact ggcgcaagag agattctact
     7741 gtaggtcttg aattgaggaa ttccacggct gttctggcga ctcctgtagc gctggcataa
     7801 gactcaaggc ttccatataa gccggtgccg gggtgatatc ttccttctgg aataatcata
     7861 gtatgcccca gctctcccgc aaatccatcg tgtccgtata tcatatgtcc attagctacg
     7921 ataccgcttc cgacacctgt gccgagggtt ataacgataa agtcacgcat tcctcttgca
     7981 gcaccatagc tcatctcgcc aagggcagca gcgttggcgt cattggtaag gattacatcc
     8041 atatcaaatt tttccctgaa catttttgcc agggggataa cccctttcca gctcagattt
     8101 ggcgcatatt ctatcgtgcc attgtaaaaa tttccattgg gggctcctac tcctatcccc
     8161 ctgattcttg ccggaccgcc ggcgtactct atcatgggca ggatggaaag gtacaattca
     8221 tcaatgaatg gagctacttc agcatgcttc cttgttttta tttcaccccg atggaatacc
     8281 ttaccattcc ggttgactat accgaatttg gtattggtgc caccgatgtc gatgcctatc
     8341 gcgagatcct ctgtgctact catttatcta cttctccaga ttgattgttt aatgcccccc
     8401 acttactact ccgtgatcat agtcgattcc ctgggctttc aatatatttt ttgtacgaat
     8461 agcataaaaa gctaaatatg caaaacacaa tacacctgct atataagaat attgaattcc
     8521 tgttacatgg tctgataaat atccctgaaa gagacttacg aatgccccgc ccataatcat
     8581 catgatgagt agacttgagc cctcattggt gtgcttgccc aatccggcaa tagcaagggt
     8641 aaatatacaa ggccaaagtg tagaacagaa gagaccaact gatataaagg caaatgtact
     8701 tacccagccg tcagccagca ttcctgcaat caaggcgatt atacccaggc aactgtatat
     8761 aagtagttgt ctggccggat ttcctttact catgatattg cctgcgacca ttgccgctat
     8821 gataaataca tagaaataaa atggcgttat atcgtgattt gcgattttgt tgaccaacaa
     8881 aaagactcca aaagcgatat aaggcataaa ataccgcaac atccttttca tgctcattga
     8941 caggtcgaac gcttcgctgg cagctgtcca tcgtcccatc attaggctgg cccaataaag
     9001 agatatatag ggcgcaatct tatctgtcgg gaaaactcct ccatcaggtc cggctatatg
     9061 ctgcttcatg aattcaggta aattagaggc tgtagatact tccactccca cataaacaaa
     9121 gatagccagc atcccgagtg aaagttgagg atatttcatg gggcctgaga ggtgccgaac
     9181 ttttgaacta tcgccttcag cttcattttc tgccatctgg tccagatcaa tttcattggg
     9241 tacctttgaa aacttgatga gtaatgcaac gagaataaat gctgcaccca ggattagata
     9301 tggaatcttt atgctggtga tatccgcctg agctgttgca gcatagatcg agccaaatat
     9361 ggcaaaattg accaaaagag ggccgattgt tgtcccaaaa ttgtttacgc ctcctgccat
     9421 acttagtcgc tgtgaaccaa gcttgggatc gcccatcacg atagctaatg gattggcagc
     9481 agtttgttgc agggagaaac ctaaccccac aatgaaaaga cctgaaataa acaagttaaa
     9541 agaatgtgta ttggctgccg gtataaacat taatgtgccc aatgcagaga tgacaaggcc
     9601 tatggcgata ccgtttttat aaccgatctt gttcaatatg tcaccccctc tcatcttact
     9661 aataaagaaa taaatcaggg atccgactgt atatgccgta tagaaggcaa aagagaccat
     9721 ctggctctgt gtctgactta gatgaagatt ttccttgaaa accggaatta agatatcatt
     9781 gctggcagca acaaaacccc agaaaaagaa tacggtaatc agagtcagaa attgtgacca
     9841 aatggtcccg gattggttat tgctcatttt tgatggtaaa tatcctgtga gttaatctat
     9901 attaaattcg tgctaagata aaaaaaatga taccaaacac cggattttat ccattttagc
     9961 aaaaaaaatt ttgatatcta ttgattttca aacgattatt attttgattt tttggtactc
    10021 ttcaatttgc ctcagataca cccctgcctt ttggccatgt tagacccatt atcattcaaa
    10081 ctaatcgggt tctgaccaga taatggaggg ggcctcattg atttgcccga aaacaaaggc
    10141 tttttgcttg aatttttggg atagaattcc tgactctacc aggctgatcc cgaggataag
    10201 caatgaaatt tcttccttcc agccattgct gccccgacca tagccttcca catattgtag
    10261 ccctaatcgg ctgacttcga caataagcct gttatgagcc tccctgttca gcctgcctaa
    10321 tgtctcaatg ccattgggat tgtatgcagt gatgaatgag aatgtattga caccataatg
    10381 tgccaatacc tcatttaacc ttgagttttg tattccgggc cggatctcaa gatctaacga
    10441 aggaacaaga taggttgtac tgttgtagat ttttagtagc tcttccttca ttcacttatc
    10501 tgcactacat cattcaaatg taaggatgga tttaattttt attgataaga atagcataat
    10561 aataaggatt tgactgatcg gtaatcataa gctttcctat ttgtttggct agcaacgtaa
    10621 tgatttcaga tccaaatccg gtttgtggct ggatgcggat atcgacagtg ccattgtccc
    10681 aatattcaac cttgatttta tcatctattt cggtgaaatt gatatagatt gccagcggtt
    10741 ttccacttgt atttgcatgc tttatactgt tggtaatcca ctcattaatg atgagggcaa
    10801 gtgaaaaact ttgctttaaa ggtatttcca catcattgaa atcaagattg gttttgatct
    10861 catggctata ggaaaaacaa tccttgatga tttctatcat tcgttcaatg aaatcccgaa
    10921 aatccaccag atatgtctcc cgcatcattt cttcatgaag aacagcaata ctttctatcc
    10981 taagtcgcgc tgcccttaga ttctcgatgg ttgccttgac agttgctttt ctttcctgca
    11041 tttgaagcaa actgaatatc atgtgcaggt tgttcttgat tctatggtgc aactctttat
    11101 acagcaggga gattttatca tgagattctt ccagttcttt ggataccctg atgtgcttga
    11161 gccgacttcg cccgatgatg acattgagat atatcgaggc taatgacagt aaggccaagg
    11221 cagtgatgga aacaaaaagt atgatgaagg atttggtctc tatctgattt tcctgttcca
    11281 aagcattcaa ctccatttct gccagctttt gaccgagatt gacatactca tgcttattga
    11341 tgaatctgaa tgtcatatca tagttcaagc cactataata aagagatttc ttgaagttgc
    11401 ctgttttttc ataaaattta ctaaggacag atgcataaaa catgctgtca ccggtggttc
    11461 gggcctcttg aaaaacctgc tccaaagtat ccagataact ggatgtcgaa atattctttt
    11521 gaatctgaat gactgcccta gtataataat aatgatcctt gtgatttaag gtaagcgtat
    11581 cgttttttaa attggattta ttgacatact tatccaatat tgtctccgcg agatcaaaat
    11641 caccggcatt ggctgcctcc attgctactt ccggtaaaaa ccttatttca ttggctacag
    11701 aaccgggcag tgtatcgatt atcccgagaa tttcattaat ctgcttgata cagttggtgc
    11761 tatcttttac tccattatag gcatgagcaa catggaattt catatataag gcggcctcaa
    11821 cggaatctga tttcgcaaag agctcttccg cttttttata ataagcgagt gtaagagcgg
    11881 gattggaatt ccaggcgtca aagaccttgc cataagcata agtcgcttcg gcaagcaaca
    11941 tggtgtcttt gagatgttcc gctatcaaca tagcagaatc aaaataattt ttggccttgc
    12001 cgaatacctt cagatcggac ttcttactat aaatttcgtt gcctttctca atgtattccc
    12061 agaaattctt cttagtatca aaatgtattt catccggttt cgtcttttta cagcccgtag
    12121 taatgatgca cagagccaaa ataaaaagaa agattgcaaa tctggaacga aatatcatcg
    12181 ggtagtaaaa tagggtacat tgcgtgatta caaatataag agccaatttc gacaaatcaa
    12241 tgtatatggt gaaatatttt catatttgac ttgaaatgga tattttcccg tcagttgtga
    12301 atatatgcag gattcaaatc acagctacaa ctcttcttaa gttttgtttt tttatctgaa
    12361 tagaaaaaaa atttgcccat tcaaaaattt gtttacattt gcgccgtagt ttttacaaaa
    12421 ccatgaaaaa tataactatt atttcttcca ttattattga gattgcggta acacgatctt
    12481 agggagagta ttgtaattaa taaaataaat gtaaaggcct cccgacgatt cggggggctt
    12541 tttttgtaaa taaaacatat gggattaata tacatcatta ttatcagcac tattattatt
    12601 cgctctaccg ggtgagaatg gctgattcta tgtatgacct aagggccttt ctcaccaagt
    12661 gggaaaggct tttttctttt taggcaccca atctatcttt tcattaatca aataaacaag
    12721 gtaaaaaagt aaacaaagga tttccatgca accaatcaat aaatacagca gaaccgtcac
    12781 ccaggatgat acccagcctg ccgctcaggc catgctttac ggcataggat taactgaaga
    12841 agacttaaaa aaagcacagg tcggtattgt tagtaccggg tatgagggta atacgtgcaa
    12901 tatgcacctt aatgacctcg ctaaatcggt aaaatcaggc gttttacaag aagatctggt
    12961 cggtctgatc tttaatacta taggtgtaag tgacggcata tcgaacggta ccgcgggaat
    13021 gcgattttca ttggtgtctc gtgatatcat cgctgactcg atagaaacag ttgtcagtgc
    13081 tcaatggtat gatgccgtag tggcagtagc cggatgtgac aagaatatgc cgggcaccat
    13141 catggctatg ggtaggctaa acaggccatc cctcatggtg tttggcggca ctatacattc
    13201 aggacaatgg aagggacaat cactcaatat tgtctctgca tttgaagctc tgggtaaaaa
    13261 aatcaatcat gacatcacag ttgaagattt taaaggtatc atccgccatg cctgccccgg
    13321 tgcaggtgcc tgtggtggta tgtacactgc caataccatg gcctctgcca tcgaagctct
    13381 gggtatgtgc cttccctaca gttcgtctgc tcctgccatg agtgccgcaa aacaggatga
    13441 atgccttgca gtaggtcgtg ccgttagaaa cctgctcgaa aaagatataa aaccatctga
    13501 tatcat
//
LOCUS       DAOQQN010000027        13369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1561_length_13369_cov_5.116269, whole genome shotgun sequence.
ACCESSION   DAOQQN010000027 DAOQQN010000000
VERSION     DAOQQN010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13369
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1561_length_13369_cov_5.116269"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PLG24_01830"
     CDS             <1..478
                     /locus_tag="PLG24_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HMZ23650.1"
                     /translation="THHNYTRKSLPEIRNFMLKVAQVHGENHPELAEMRKLILRVHDE
                     MMDHLLDEENDLFPTIKELVKAKNNDGNCSVHSGQTFEAMIAIAEEEHDIVGRSMEKV
                     RHLSNDYTIPSDACTSYKLLYKMLEEFENDLFIHIHLENNILFPKAIEIERSIVSH"
     gene            686..1504
                     /locus_tag="PLG24_01835"
     CDS             686..1504
                     /locus_tag="PLG24_01835"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HMZ23651.1"
                     /translation="MLINEKTKIAALLKYHPDALETIVSIAPDFKKLRNPILRKLMAG
                     RTSIAMASKIGGVRPEDFFKALKPLGFEYDDTGGPVEEIFEESFMPDYLKNLDEDRLV
                     SFDVRELIARGDDPLRSIQDKVKSLSPGQVLLIINSFEPVPLIKLLERQGFKAYVKQV
                     DGDTYETYFYKEGESGSRISENDAAISTSGDWEEMLTQFENRIEEIDVRHLEAPMPMI
                     TILEALDSLPEGKALYVHHKRIPVFLLGELNERRFGYRIKEVSENMVYLLIFKE"
     gene            1511..2788
                     /locus_tag="PLG24_01840"
     CDS             1511..2788
                     /locus_tag="PLG24_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23652.1"
                     /translation="MFGPGIVGLQQTTNYRVVLPFYIYASVSFLAGTLLLFLRTDIAN
                     DHYFYPPTLAITHIMALGWGTMIILGASHQLLPVLIEGKLYSNRLAIMTFGFTCLGIP
                     LLVFGFYIFRTGWIMQLGALLVNLGVLLYVSNVFLSAFKSEKRNVHAWFIITASLWLL
                     ATTTFGLLLVINFDHTIFPKESLAYLSVHAHLGLVGWFLLLVIGVGSRLIPMFLISKY
                     TNEKALWTIYGLVNAGLIVFILARMVNRSLEFLAIIPILTGIVVFGHHCFKAYAVRIR
                     KAVDEQMKASLLSIIHMLLPFVVLIMSLWVLPSGGQPNLVLLYGFSILFGWITAIILG
                     MTFKTLPFIVWNKVYHKKAYTGKAPLPKDLFSEKMYHYMLMAYILGFGVFVIGIILLN
                     QWLLKIGAAALLMTAILYVLNTTKILIHKPRKI"
     gene            2785..3114
                     /locus_tag="PLG24_01845"
     CDS             2785..3114
                     /locus_tag="PLG24_01845"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HMZ23653.1"
                     /translation="MSVNIETNNPEKCDYALIGLYEVYDPEVGLNVVDLGLIYELDFD
                     EDNKSINCRMTLTTQFCPMGESITSDIMQSLTDAFGDYQINVNLTFNPPWSFEKISPE
                     GRAFLGR"
     gene            3117..3545
                     /locus_tag="PLG24_01850"
     CDS             3117..3545
                     /locus_tag="PLG24_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HMZ23654.1"
                     /translation="MLSNTSKTAIKAVIYLAGKSECTKASIREVADHINASEHTVGKV
                     LQTLARHKIITSLKGPSGGFFLSDQQKFQPISVIIESIEGKGVFKECGLGLSKCSEIH
                     PCPIHDEYKIARGLMERIFREKMIKDLHDPVTDGLAFLMD"
     gene            3589..4344
                     /locus_tag="PLG24_01855"
     CDS             3589..4344
                     /locus_tag="PLG24_01855"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HMZ23655.1"
                     /translation="MTLEKNENGELDVRVLIPIQRHELLVGLFKDLPTHENFVFINDH
                     DPIPLYYEFKSIYGDVVGWEYLNRGGREWKVRVTRTEDSQARDMSGIATLLDLRNADK
                     KEWKQVVFHRYGMMEEGTTMELIAREDLQEIHGIFKSKFAGMHTWMYKKEVPDEYVIH
                     IRKEAKSGLGDDGYSVVNEFDVRPYPPTERHEMFYKAFGDIKPGEAFEFVNDHDPKPL
                     YYQMEAESKEPFRWEYLEKGPEVWKVRVVKVEK"
     gene            complement(4450..5547)
                     /gene="ychF"
                     /locus_tag="PLG24_01860"
     CDS             complement(4450..5547)
                     /gene="ychF"
                     /locus_tag="PLG24_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HMZ23656.1"
                     /translation="MALKCGIVGLPNVGKSTLFNALTSAKALAANYPFATKEPNLGVI
                     TVPDKRLDQLSDIVRPQKVQPTTVEILDIAGLIKGASKGEGLGNQFLANIREVDAIIH
                     VIRCFQDDNVVHVDGSINPVRDKDVIDTELQLKDIETLEKRLDKIQKAAKTGDKAAKA
                     TFDLGQRMLAHLGDGNNARSLDLTEEEALEIKEFQLLTGKPILYVCNVDEDSAVSGNE
                     YSAAIKELVAAENAQVVIISAGIEADIAELETKEERLEFLEAIGLSEPGVNKIISAAY
                     RLLGLITYFTAGEKEVRAWTITEGTKAPGAAGVIHTDFEKGFIRAEVIAYEDFISYGS
                     EAAVKEAGKMGVEGKEYIVRDGDIMHFRFNV"
     gene            complement(5794..6645)
                     /locus_tag="PLG24_01865"
     CDS             complement(5794..6645)
                     /locus_tag="PLG24_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HMZ23657.1"
                     /translation="MSIAASELILNEDGSIYHLGLHPEQVYPTIITVGDPDRVEKVSK
                     YFDRIDFKKQKREFISHGGRIGNRDITVVSTGIGTDNVDIVINELDALVNIDLDTRTP
                     KSDIRKLDIMRLGTSGTIRAEVEVDSLVLSRGAIGMDGLMHFYKRDKKPEELEMLTWE
                     INWPLPPYYYSGSESLLARYTYLGIEGITVSNTGFYGPQSRRLRLDPASDNLFETLAG
                     QSINGCPITNLEMETSGIYGLCQLLGHNCLSINVIIANRSLGKFSNAPDKAVINMIET
                     ILEEASK"
     gene            complement(6636..7112)
                     /locus_tag="PLG24_01870"
     CDS             complement(6636..7112)
                     /locus_tag="PLG24_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23658.1"
                     /translation="MKTINLIIFLLLIQWSCLKAPEYPIEPIISFISVSNSVIKQSQF
                     NGDSTYITFGFTDGDGDLGDQDSVNIFVTDQRTNIHPTVYKIPFLKQNNGAKGISGEI
                     RIRLFSECCIYPNGQVPCTPSTEFPTDTLIYSIYIKDRAGHQSNIIYTTPIVLLCQ"
     gene            7246..8247
                     /locus_tag="PLG24_01875"
     CDS             7246..8247
                     /locus_tag="PLG24_01875"
                     /inference="COORDINATES: protein motif:HMM:NF016338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HMZ23659.1"
                     /translation="MADNILPEEILKLSRQLVMGEIILVNEVYPVIFDFQSKFNPVYR
                     SYLKQLKFDLKYPELEDIPFLPITFFKNFRITSCDWQEQTVFRSSSTTGKGRSSHYIR
                     DLQIYKTVSLKIFEKYFGPISHLRIMALLPGYLEREDSSLVYMVNYLMGCTAVDQRYF
                     FLDRYHDLMEVVQGNLDEHIILFGVPYAFIRCFRELGYQFWPNVTMIETGGMKGKEAE
                     MTKAELHLFLEESFRPKSIYSEYGMTELLSQCYSKGEGKFEVSDTMYMIVKELNDPFS
                     IQKRGKPGVLNVFDMANVHTCSFIETEDMAILHENGEFEILGRVDNREIRGCNLLAG"
     gene            8295..8786
                     /locus_tag="PLG24_01880"
     CDS             8295..8786
                     /locus_tag="PLG24_01880"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HMZ23660.1"
                     /translation="MIQVFVTGGTFDKEYNFITGELYFKDTHLNTMFVRGRSSLEIDV
                     KTLMMVDSLEMTEEDRNIIVYQCKKSPAEKILITHGTDRMTATARAIAEAEIEGKTIV
                     LTGAMVPYAFGNSSDGFFNLGAALAFVQVLPPGVYIAMNGKYFPWNNVRKNYDTGFFE
                     EIE"
     gene            complement(8850..9401)
                     /gene="hpt"
                     /locus_tag="PLG24_01885"
     CDS             complement(8850..9401)
                     /gene="hpt"
                     /locus_tag="PLG24_01885"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HMZ23661.1"
                     /translation="MESIKIHDKSFVPFILKDDIDNRIAGIASELENKFKNKQPVFLI
                     VLNGASFFGVDLLKKYRQPCSIQFLRLKSYEGTDSTGNVKSIGNMPENLKGRDIVILE
                     DIVDTGLTMNFLKYKLEEEGVGSITIVTLLDKPSRRVQDVKVEVVGFTIPDVFVVGYG
                     LDYDDLGRNTEHIYKLSDNIKSE"
     regulatory      9562..9654
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9700..12150
                     /locus_tag="PLG24_01890"
     CDS             9700..12150
                     /locus_tag="PLG24_01890"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HMZ23662.1"
                     /translation="MFQSLCFKRMAIAWIAMWLMINLNAQKTVVIVRDYAGKGVEDAS
                     VSVNGYGAITDHEGRVEGEFRFPLHVVVTYVGTNGLDTIISEQTSKVELILLGTGILL
                     KEGLVTASWANESSAVTQKTLSKSRIYSDNPADMPSILESLPGSVSTSDAGNGIGYSA
                     LRIRGSDQTRINVTIDGVPVNDAESQDIFWVDLPDIIEDVESVQIQRGVGVSTAGPGA
                     FGANINLRTGMLSKIPLLTANVGYGSFNSQKYGLALSSGVLGKYFRFKIRGSRISSDG
                     YMDRASSQLWAGSFQSQYVRQRFSLAANVYWGKEETYQAWEGTPIQFYKADPGTTFNP
                     AGLKSDGTYYSDQKDFYRQVYSRLIGRWHIKQNSGLQFTLYNTLGKGYYNQYREENIS
                     KYFDDVPSMDVALIRERWLDNVLNGLNLVFDHQNNRWFLQTGGNLQLYRGKHFGIIKN
                     YQGLPDWVPRYYYDNIGEKQEASVFARIEKTLDRIGAFVDLQGRRIAYTYEGLDNEWN
                     PEAITQRFLFFNPKVGATIFLNENKDRNIYIFGGVANKEPNRRDFTDAAPGKLPKPER
                     MYNGEMGYRHSKQNFDLQSNIYFMFYQDQLVLTGKINDVGAYTRFNVDKSYRMGWETD
                     LRWQPFQQLTILGNISLSKSKIIDFTEYSDVSILTDEGVEYPGQVATFKGNTDIAFSP
                     FVVAYGKMSYQPFMQSKQLKGLSFHYTYKFVGSQYIDNTSDIHSKLPSYNLHGMGFSL
                     PLRRGKNGFVLSGNIDNLLNIHYVNNGWIYRFKAIGYDASKDDPYIQSEGNDYYSSVG
                     YFPQAGRRVFVSLRYMLK"
     gene            12380..>13369
                     /locus_tag="PLG24_01895"
     CDS             12380..>13369
                     /locus_tag="PLG24_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HMZ23663.1"
                     /translation="MNFKIFLLIPWMVIYGFFAFAQSPGKLNIIPQPVKVHELKGSLN
                     LRNYPAYLYGPGIEKDPMFDFNGWASKEFGLEMKRVMQPGNAIKFVKVDTFSHPDAYL
                     LTIDDNGVLIQFKDEAGAFYATQTLKQLCPVESSDIGTNSGVPMSVPYCTIYDYPRFS
                     YRGMHLDESRHFFGKDAVKKYIDMLAFQKYNYFHWHLTDDQGWRIEIKRYPKLTEISS
                     CRDETLIGHYSDKPVRYDGNKYCFYYTQEEIKEIVVYAARKYITIVPEIEMPGHASAA
                     LAAYPELGCTEKQIRPATTWGIFDDIFCPKEGTFFFLQNVLDEVMELFPSKYIH"
BASE COUNT         3902 a         2362 c         2978 g         4127 t
ORIGIN      
        1 tacacatcac aattatacca gaaaatctct accggaaatc aggaatttta tgctgaaagt
       61 tgcacaagtt catggggaga accatcccga actggcggaa atgaggaaat tgatcctccg
      121 tgtgcatgat gagatgatgg atcatttgct ggatgaagaa aatgaccttt tccctactat
      181 aaaggaattg gtcaaagcga agaataatga tggtaattgt agtgtacatt cgggacaaac
      241 atttgaagcg atgatcgcca ttgctgaaga ggaacatgac atagtaggta ggtcaatgga
      301 gaaagttaga catttgagca atgattatac tattccgtca gatgcatgta ctagctataa
      361 actgttatac aaaatgctgg aagaatttga gaatgatctg ttcatacata ttcatctgga
      421 gaataacatc ctttttccaa aagctataga aatagagcgt tcaatagtca gtcattaacg
      481 ataaaacagc taattcagga catatcgaat gaaatggaga ctctttgagc tatccggggt
      541 tgatgaattt tatataaaga tttttttaag aatgtaacct tgcagaggta tttatgacga
      601 agggcattat tagaatttat gtatttgtcg acctttaatc atcaatgtca agtccgaaag
      661 ttttaatgtt ttcaaataac ataaaatgtt aatcaacgaa aaaacgaaaa tagcagcact
      721 tttgaaatat catcccgatg ctttggagac catcgtttcc attgctccag atttcaagaa
      781 gttacgtaat ccaattctgc gcaaattgat ggcaggtcgt accagtattg ctatggcctc
      841 taagattggc ggtgtcaggc ctgaggactt tttcaaggcg ttgaagccac ttggctttga
      901 gtatgatgac accggagggc cggttgaaga aatatttgaa gaatcattca tgcctgatta
      961 cctcaagaac cttgatgaag ataggcttgt gagtttcgat gtgagggaac taattgccag
     1021 gggcgatgat ccgttgagat cgattcagga taaggttaaa tccttatcac ccgggcaagt
     1081 actcttgatt atcaatagct ttgaaccggt tccgcttatc aaattgttag aacgacaggg
     1141 ttttaaagct tatgtaaagc aggtcgacgg ggatacttat gaaacctact tctacaagga
     1201 aggtgagtca ggaagcagga tatcagaaaa cgatgcagct atttcaacct caggtgattg
     1261 ggaggagatg ttgacacaat ttgagaatag aattgaagaa atagatgtgc gtcatctgga
     1321 ggcacctatg cctatgatta ctattctgga ggctttggac agtcttccgg agggcaaggc
     1381 actgtatgtc caccacaagc gcataccggt cttcctttta ggcgaattga atgaacggcg
     1441 gtttggatac cggatcaagg aggtgtccga aaatatggtt tatttattga tattcaagga
     1501 gtaatagcag atgttcggac cgggaattgt aggattacaa caaacgacga attacagagt
     1561 ggttctacca ttctatattt atgcatcagt atcattttta gcgggtacat tgttattatt
     1621 tttacgtaca gatattgcca acgatcacta tttctatccg ccgactctgg ctattactca
     1681 tattatggct ttgggatggg gcacaatgat catcttaggt gcttcacatc aactattgcc
     1741 tgttcttatt gaagggaagc tttatagtaa ccgattggct attatgacat ttggattcac
     1801 ctgccttggt attccattac ttgtatttgg tttttatatc ttccggaccg gatggattat
     1861 gcaattaggt gcattattgg tcaatctggg tgtattgttg tatgtatcta atgtcttcct
     1921 gagtgctttc aagagtgaaa agcgcaacgt ccatgcatgg tttatcatta cagcatcttt
     1981 gtggctgttg gcgacaacaa ctttcggcct tttgcttgtc atcaactttg atcatacgat
     2041 atttccaaag gaatcccttg cctatctttc ggtccatgca catctgggac ttgtaggctg
     2101 gttcttgttg ttggtgatag gagtgggctc caggttaatc cctatgtttc tcatttcaaa
     2161 atataccaat gaaaaggcac tttggacgat ttatgggctg gtaaatgcag gtttaattgt
     2221 attcattctg gcacggatgg ttaaccgttc tttagagttt cttgcgatta taccaattct
     2281 cacaggtatt gtcgtattcg ggcaccattg ttttaaagct tatgccgttc ggatcagaaa
     2341 ggcggtcgat gaacagatga aagcgtcctt actttcgatt atacatatgc ttttgccctt
     2401 tgttgtgctt atcatgtcac tttgggtact gccttccggt ggccaaccta atttggtatt
     2461 gttatatgga ttcagcatac tttttggatg gatcacagcc atcattttag gcatgacatt
     2521 taaaaccttg ccattcattg tctggaataa agtataccac aaaaaggcat atacggggaa
     2581 agcgcctctg ccaaaagatt tattttcaga gaagatgtat cattatatgc ttatggccta
     2641 tatattgggc tttggagtgt ttgtgattgg catcatcctt ttgaatcaat ggttactgaa
     2701 gatcggagct gctgcattat tgatgactgc gattctgtat gtgctcaaca caactaaaat
     2761 tttaattcat aaaccccgta agatatgagt gtaaatattg aaaccaacaa tcctgagaaa
     2821 tgcgattacg cattgatcgg cctttatgag gtctatgatc ccgaagtagg cttaaatgtg
     2881 gtggaccttg gtctcatcta tgaattggat tttgatgagg ataacaaaag tatcaactgt
     2941 cggatgacat tgacaacaca gttttgtcct atgggtgaaa gcatcaccag tgatatcatg
     3001 caatcactga cggatgcctt cggcgattat caaatcaatg ttaacctcac cttcaatcca
     3061 ccctggagtt ttgaaaaaat atcgccggaa ggtagagctt ttttaggaag gtaattatgt
     3121 taagtaatac gagtaaaaca gcaatcaaag ccgtcattta tcttgccggt aaaagtgagt
     3181 gtaccaaggc gagtatacgc gaggtggctg atcatatcaa cgcgagtgaa catacagtgg
     3241 gcaaagtttt acagacactt gccaggcata agataattac cagtctgaaa gggccttccg
     3301 ggggtttttt cctgagtgat caacaaaaat tccagcctat ctccgtaatt attgagtcta
     3361 ttgaaggaaa aggtgttttc aaagaatgtg ggcttgggct tagcaaatgt tccgagatac
     3421 atccctgtcc aattcacgac gagtataaaa ttgccagagg cctgatggaa aggattttcc
     3481 gggaaaaaat gattaaggac ctgcatgatc ctgtcacaga tggtcttgca tttttaatgg
     3541 attgatataa ggtattgtaa taaaaggtat tataaatata aatttagtat gactttagag
     3601 aaaaatgaga atggtgaact ggatgtacgt gttttgatcc cgattcagag acatgaatta
     3661 ttggtcggat tgttcaagga tctacctacc catgaaaact ttgtctttat caatgaccat
     3721 gatccaattc cactgtatta tgagttcaag tcgatatatg gagatgttgt tgggtgggaa
     3781 tatctcaatc ggggaggtag ggaatggaag gtccgggtga cccgaaccga agactcgcag
     3841 gcacgtgata tgagtggaat agcaacgtta ctcgatctgc gaaatgcaga taagaaggaa
     3901 tggaagcagg ttgtttttca ccggtacgga atgatggagg aggggactac gatggaattg
     3961 attgccaggg aagatcttca agagattcat ggtatattca aaagcaaatt tgccggaatg
     4021 cacacatgga tgtataagaa ggaggtgccg gatgaatacg tcatccatat ccggaaggaa
     4081 gcgaagagtg gtttaggtga cgacggttac tctgttgtta acgaattcga tgtcaggcca
     4141 tacccaccaa ctgaaaggca tgagatgttc tacaaagctt ttggggatat caagcccggt
     4201 gaagcatttg agttcgtcaa tgatcatgat ccgaagccgc tttattatca gatggaagcg
     4261 gagagtaagg agcctttcag atgggaatat ctggagaagg gccctgaagt atggaaagta
     4321 cgtgtggtta aggtagaaaa ataaagacag tgaatctgga ggttacaagc cgcagatcca
     4381 ctgttcacgt atattgtttt gattaggatg ggtgggattt aatttttatc aaaaaaccgt
     4441 tttaatgaat tatacgttga agcggaaatg cattatatca ccatcccgaa caatgtattc
     4501 tttgccttcc acgcccatct tgccggcttc ctttaccgct gcttcagagc cataggaaat
     4561 gaaatcttcg taagctatta cttcagcgcg aataaagcct ttttcaaaat cggtgtgaat
     4621 tactcctgct gctcccggag ccttggtgcc ttctgttatt gtccaggctc tgacttcttt
     4681 ttcaccggct gtaaaatatg taatcagtcc gaggagtcta taggcagctg atatgatttt
     4741 gttgacaccc ggctcagaca ggcctatagc ctctaagaac tccagtcttt cctctttagt
     4801 ctccaattca gcaatatcag cctctatccc cgctgaaatg ataactacct gtgcattttc
     4861 agctgccacg agttccttga ttgcagcaga atattcattg ccggagactg cagaatcttc
     4921 atctacattg caaacatata aaatgggctt tccggtcagg agctggaatt cctttatttc
     4981 caatgcctct tcttcagtca gatccagtga tcgggcatta ttgccatctc ctaagtgagc
     5041 gagcattctt tggcccaaat caaatgtggc tttggctgcc ttatctcccg ttttagccgc
     5101 tttttgaatt ttatccagtc ttttttccag tgtttcgata tccttaagtt ggagttcagt
     5161 atctatgacg tccttatccc gaactggatt gatggaacca tcgacatgca cgacattgtc
     5221 atcctgaaag catcgtatga catgaatgat ggcatcgact tccctgatat tggccaggaa
     5281 ttgattgccg agtccctcac ctttgctggc tccttttatt agccctgcga tatcgaggat
     5341 ttcgacggtg gtcggttgca ctttttgagg ccggacaata tccgacaatt gatccaatcg
     5401 tttatcagga acggtgatta caccgagatt aggttctttg gtggcaaagg gataatttgc
     5461 tgcaagggct tttgctgagg ttagggcatt gaagagggtc gatttgccta cattaggtag
     5521 tccgacgata ccgcatttga gagccatatt ttattttgtt tttggccgcg aagatacatt
     5581 taatttcagg cataaaactg tctttgtcaa aaatctttta tttagcgata tctatgagcg
     5641 atgcttatat atcttcaaga agcttcttta tcgggaggat tgcatatttc cgggctgaaa
     5701 gagaaaaagg gcgatccgat tgattttaag ggtacttgat acaaaggtaa taaaagtaat
     5761 attgagcatc aatatcatta aataacgtat aatttattta cttgcttctt ccaatattgt
     5821 ctcgatcata ttgatcaccg ccttgtcggg tgcattactg aatttaccta aggatctatt
     5881 cgctatgatt acattgatgg acaggcaatt atggcctaga agttgacaaa gtccgtatat
     5941 gcctgatgtc tccatctcaa gattagttat cgggcatccg ttaatacttt gaccggcaag
     6001 ggtttcaaat aagttgtccg atgcagggtc aagtcgcaac cttcggcttt gaggaccgta
     6061 aaagccggta ttagatacgg ttatcccttc aataccgaga tatgtgtacc ttgccagcaa
     6121 actttcagac ccggaatagt aataaggtgg caaaggccag ttaatttccc aggttaacat
     6181 ttccagttct tcaggctttt tatctctttt gtaaaaatgc atcaggccgt ccatccctat
     6241 tgctccgcga gacaggacca gactgtccac ttcgacctct gctctaatag ttccggatgt
     6301 accaagcctc atgatatcca gtttcctgat atctgatttt ggtgtcctcg tatccaaatc
     6361 gatattgact aaggcatcca attcattgat gacgatatca acattgtctg ttcctattcc
     6421 agttgagact acagtaatgt ccctgttacc tattcggcct ccatgcgaga tgaattctct
     6481 tttttgtttt ttaaaatcaa tccggtcgaa gtatttgctt actttttcta cccgatcagg
     6541 atctccgaca gtaatgatgg tcgggtatac ctgttccggg tgtaatccga gatgataaat
     6601 acttccgtct tcattgagaa tcagttctga agctgctatt gacataataa tacgattggc
     6661 gttgtgtaaa tgatgttgct ttggtggccg gctctatctt taatataaat actatatatc
     6721 agggtatcgg ttggaaattc tgttgaaggt gtgcatggga cttgtccgtt agggtaaata
     6781 cagcattcag agaataacct tattctaatt tctcctgata tacctttggc accattgttc
     6841 tgttttaaaa agggaatctt gtacacggtt ggatgtatat ttgtccgttg gtccgtcacg
     6901 aagatgttga cggaatcctg gtccccaaga tcaccatctc catccgtaaa tccaaaagtg
     6961 atataagtgc tgtctccatt gaactgactt tgtttgatta cggagttgct gactgaaatg
     7021 aaactgatga ttggctcaat gggatattcc ggtgctttca gacaagacca ctgaatcaaa
     7081 agaagaaata ttatgaggtt gatggttttc atcatatata attaaataca tccaacggcg
     7141 taaaatttac aacaaatttg cttcaccaga taatccaata aattttccct gtgattgggg
     7201 ttaaatatac atatttgcat tgtaaaccga aattcttttt gcttcatggc cgataatatt
     7261 ctcccggaag agattctaaa actcagccgc caactggtaa tgggtgaaat aatattggtc
     7321 aatgaagtat atcctgtcat atttgatttt cagtcgaaat ttaatccggt atatcgctct
     7381 tatttaaaac agttgaagtt tgatttgaag tatcccgaat tggaagacat tccttttttg
     7441 cccattacat ttttcaagaa tttccgtata acatcctgtg attggcagga gcaaacagtg
     7501 ttcagatcca gctctacaac cggaaagggg agaagtagtc attatattag agatttgcaa
     7561 atatataaaa cagtcagtct gaaaatattt gaaaagtatt ttgggccaat cagccatctg
     7621 cgtattatgg cacttttacc cgggtatctt gagcgagagg atagtagtct ggtctatatg
     7681 gtaaattatt taatgggatg cacggctgtg gatcaaaggt atttttttct tgaccgatat
     7741 catgatttaa tggaagtggt acaagggaat ttagatgagc atattattct tttcggcgtt
     7801 ccctatgctt ttattcggtg ctttagggaa ttaggctatc aattttggcc gaatgtgacc
     7861 atgatcgaaa ccggggggat gaaaggcaaa gaagctgaga tgacaaaagc cgagttgcat
     7921 ttatttctcg aggaatcctt tcgcccaaaa tcaatttatt cggagtatgg aatgacggaa
     7981 ttactttccc aatgttattc gaaaggcgaa gggaaatttg aagtgagtga tactatgtat
     8041 atgatagtga aggagttgaa tgaccctttt tcaattcaaa aaaggggtaa gccaggtgtt
     8101 ttgaatgtct ttgatatggc taatgtgcat acctgttctt ttatcgaaac ggaggatatg
     8161 gccatccttc atgaaaacgg tgaatttgaa atcctgggtc gggtagataa tcgggagata
     8221 aggggctgta atcttcttgc aggatagggc ttttaccgtt tttttttaat atgtttgcat
     8281 taaatatttc aataatgatt caggtttttg ttacaggagg gacttttgac aaggagtata
     8341 attttattac aggtgagctt tatttcaagg atacccatct taatactatg tttgttaggg
     8401 gccgaagcag tctggaaatc gatgtaaaga cattgatgat ggtggatagt cttgaaatga
     8461 cggaagaaga ccggaatatc atagtatatc agtgtaaaaa atcacccgct gagaaaatac
     8521 ttattacaca tgggacggat aggatgactg caacggcaag agctattgct gaagctgaaa
     8581 ttgaaggtaa gacgatagtc ctgacgggag cgatggtgcc ttatgcattc ggtaattcca
     8641 gtgatggttt ctttaatctc ggagctgcac ttgcctttgt tcaggtctta ccgccgggtg
     8701 tttatattgc gatgaatggt aaatactttc catggaacaa tgtccgtaag aattatgaca
     8761 caggtttttt tgaggaaatt gaataatatt atcggatggg gtgcctattt tccattaatt
     8821 ttaaaatttt ccggaaatta ttaggcggat tattcacttt tgatattatc tgacaatttg
     8881 tagatatgct cagtattcct tccgaggtca tcataatcga gaccataacc cacgacaaat
     8941 acatccggta tggtaaagcc tacgacttca acctttacat cttgcactct cctggacggt
     9001 ttgtccaata gtgtcacgat ggtaatagaa ccaacacctt cctcttcaag tttatattta
     9061 agaaaattca tggtcaagcc tgtatccaca atatcctcta agatgacgat atcacgcccc
     9121 tttaaatttt ccggcatgtt ccctattgat ttgacattac ctgtcgaatc agttccttca
     9181 tagcttttta agcggagaaa ctgaatggag catggttgtc tgtatttttt caataaatca
     9241 actccaaaaa aggaggcgcc attcaataca atcagaaaaa ccggttgctt gttcttaaat
     9301 ttattttcca attctgaagc aatcccggca atcctgttgt cgatatcatc tttgaggata
     9361 aagggaacaa aagatttgtc gtgaatttta atagactcca atgttagtat tttaaattgt
     9421 ttgtgatcaa tgaataaagc tccctgatga gatcaactga atcaatcccg gcaaagatac
     9481 aaatacaaat ttcattcaga tacttagaaa atgttgtttt atcaatttgt aaataagtaa
     9541 aagtacggta aatttgcatc atcttattaa ctggggtgct ggtgtaaccg gctgagataa
     9601 aacccattga acctgccatg gtaatagtgg aagggatggt aatttgtgga tagaagttgg
     9661 gattaacccc atgttccagc attcataaat taaataaaaa tgtttcaatc tttgtgtttt
     9721 aaacgaatgg cgattgcctg gattgccatg tggttaatga ttaatttgaa tgcccagaag
     9781 acggttgtaa ttgttcggga ttatgcaggg aagggtgttg aagatgcatc ggtttctgtc
     9841 aacggctatg gagccattac agaccatgaa ggaagggtgg aaggtgaatt tcgctttcca
     9901 ttacatgttg tagttacata tgttggtact aatggactgg atacaataat atcggaacaa
     9961 acatcaaagg tagaattgat attattgggt acaggaattc ttttaaaaga gggtctggtt
    10021 acagcttcct gggccaatga gtcttcggct gtaacacaaa agacgctttc taaaagccgc
    10081 atttattcag ataatcctgc tgatatgccg tccatattag aatccttacc agggagtgtt
    10141 tcaacttcag atgctggaaa tggaattggg tattcggcat tgagaataag aggaagcgat
    10201 cagacacgaa taaatgttac tattgatggt gtgccggtca atgatgctga gagtcaggat
    10261 atattctggg tggacctgcc tgacataatt gaggatgtcg aaagtgttca gatacagagg
    10321 ggtgtaggtg tgtcaactgc aggtcccggt gcttttggag ccaacattaa tctccggaca
    10381 ggtatgctga gtaagatacc acttttaact gccaatgtgg gttatggttc attcaatagt
    10441 caaaaatatg gattggccct cagctcaggt gttttgggga agtatttcag attcaagata
    10501 cggggtagcc ggatttcatc tgacggatat atggacaggg catcgagtca attgtgggct
    10561 ggttcgtttc agtctcagta tgtgcgtcaa agattcagtc ttgcagctaa tgtgtattgg
    10621 ggcaaggagg aaacatatca ggcatgggag ggaacgccga ttcagtttta taaggcagat
    10681 ccagggacga catttaatcc ggccggtctg aaaagtgatg ggacatatta tagtgaccag
    10741 aaggatttct atcggcaagt gtacagcagg ttgattggtc gatggcatat caaacaaaat
    10801 agcgggctgc aatttacatt atacaatacg ctgggtaaag gttattataa ccagtatcgg
    10861 gaagagaata tttctaagta ttttgatgat gtcccttcca tggacgtggc tttgataagg
    10921 gagagatggc ttgataatgt gctcaatggg ttgaatctgg tgtttgatca tcagaataat
    10981 agatggtttc tacagaccgg tggaaacctt cagttgtatc gcggaaagca ttttggcatc
    11041 ataaaaaatt atcaaggctt gcctgattgg gtccctagat attattatga taacatagga
    11101 gagaagcagg aagccagtgt ctttgccagg attgaaaaaa cacttgaccg cataggagct
    11161 tttgtcgatt tgcaaggtcg gcgaattgct tatacttatg aaggacttga taatgagtgg
    11221 aatccggagg ctataaccca gcgattcctc ttttttaatc caaaggtcgg tgcaaccatt
    11281 ttccttaatg aaaacaaaga caggaatatt tacatttttg gaggcgttgc aaataaagag
    11341 cctaatcgta gggactttac agatgccgca ccaggcaaat tgcccaaacc ggagagaatg
    11401 tataatggtg agatgggata caggcattca aaacaaaact ttgatttgca atccaatatt
    11461 tattttatgt tctaccagga tcagttggta cttaccggca agattaatga tgttggtgca
    11521 tacacacgat tcaatgtaga taaaagttat aggatgggat gggagactga tctgcgttgg
    11581 cagccgtttc agcagcttac aattttgggg aatatctcct tgagtaaaag caagattatc
    11641 gacttcaccg agtatagcga tgtgagtatt ttaacggatg agggtgttga atatcccgga
    11701 caggttgcca catttaaggg gaatacggat atagcgttca gtccctttgt tgttgcttat
    11761 ggaaaaatgt cctatcaacc ttttatgcaa tcaaagcagt tgaaaggatt gtcattccat
    11821 tatacttaca aatttgtggg aagtcagtat atcgataata cttcagacat tcatagtaaa
    11881 ttgccatcat acaaccttca tggaatgggc ttttcattac ctttaagaag aggtaaaaat
    11941 ggatttgtat tgtcaggaaa cattgataat ttgctaaaca ttcattatgt caataacggg
    12001 tggatttacc gatttaaggc aattggttat gatgccagta aagatgatcc ttatattcaa
    12061 tcagaaggaa atgattatta tagttctgta gggtattttc ctcaagccgg cagaagggta
    12121 tttgtgagtt tgagatatat gctgaagtga ttttcccggg aattattatt cctaaaatat
    12181 tgaatatatg taggataaca agatgtattt ttggtgttaa gttatatgcg gttatttaat
    12241 aatattatta tcaaaatgat atggtaaata ttataccaaa tgaagtaact gtgtattttc
    12301 ctcccgaaat aaacatatct ttacgactat taaccttgtg agtaataatt tgattgataa
    12361 ataaaaacag acatttgccg tgaatttcaa aattttctta ctaattccat ggatggtaat
    12421 atacggattc ttcgcttttg cgcaaagtcc tggaaaactt aatattatcc ctcaacctgt
    12481 caaggtgcat gaattaaaag gaagtcttaa tcttcgtaat tacccggcat acctatatgg
    12541 tcctggcatt gagaaggatc cgatgtttga tttcaatgga tgggcaagta aagaatttgg
    12601 tttagagatg aagcgtgtga tgcagcccgg caatgccata aaatttgtca aggtagatac
    12661 gttcagccat cctgatgcat atctgctcac gatagatgat aacggagtcc ttattcagtt
    12721 taaagatgaa gcgggggcgt tttacgctac acaaacattg aagcaactat gtccggtaga
    12781 atcttctgat attggtacaa atagcggtgt ccccatgtcg gtaccatatt gtaccatata
    12841 tgattacccc agattttcct atagaggaat gcaccttgac gagagccgtc attttttcgg
    12901 aaaggatgcc gtcaaaaagt acatagatat gcttgcgttc cagaaatata attattttca
    12961 ctggcattta acggatgacc agggctggag gatagaaata aaaagatatc ccaaattgac
    13021 ggaaatttct tcttgccgtg atgaaacttt aatcgggcat tacagcgata aacccgtccg
    13081 ctatgatggg aataaatatt gtttttatta tacacaggaa gaaataaaag agatcgtagt
    13141 atatgcagcc aggaaatata ttacgattgt acctgagatt gaaatgcccg gccacgcatc
    13201 agctgcactg gctgcatatc ctgaactggg ttgcacagaa aagcaaataa gacctgccac
    13261 cacttggggt attttcgatg atatcttctg cccaaaagaa ggtacatttt tctttttgca
    13321 aaacgtcctg gatgaagtga tggagttatt tccatcaaaa tatatccat
//
LOCUS       DAOQQN010000028        13313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1577_length_13313_cov_5.232916, whole genome shotgun sequence.
ACCESSION   DAOQQN010000028 DAOQQN010000000
VERSION     DAOQQN010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13313
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1577_length_13313_cov_5.232916"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLG24_01900"
     CDS             complement(<1..357)
                     /locus_tag="PLG24_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23664.1"
                     /translation="MMDKFYSIRSNAGGGKAATFRNATTFINHRGALGISLILGLFLT
                     LSSCASLNKSLAVSSMKDVDYGMPVKYAHLSNDIEMAYSESGSGPQTILFIHGLASYI
                     PAWKKNIVSLKDHYRCI"
     gene            complement(368..2002)
                     /locus_tag="PLG24_01905"
     CDS             complement(368..2002)
                     /locus_tag="PLG24_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23665.1"
                     /translation="MIGVRIKPGDFTTWTGIVRFFQIFAGLTILLWFPLNLDAQDMTG
                     AGFKGFDKEVPQKANKELQAFVYFYTQGVSMNMYPQNDFLKGQIVGRLFGSNTTSTSD
                     SLTAFYVEQRAIPFFVYTPRIFDGKATLRASFEIDWTWGDVAYGTGGNQGSAVSADQV
                     NIQTQNLEVELAPVKNWTVNIGLQRMFDTPHDLYRTTVDKFMQTGYRFAFFGTDAVGV
                     SVRRQTDYTKTKGGFYKFYENNVEVNDDVTMFELNHQHTISPKWNVGASIYHVRDRSE
                     GRGGVSILGQGPSSPLTLYNGTYRFPLASTRYHMDATWLGGYFSRNDEMMLDRFLLTG
                     FFNYNMGKISEYAESTKKFDKTVSIGGLSANLRAGYRYGQTTGDAITAEALVTTGDAN
                     GISDGKYSGVVTANTWGSPAAIFVGSGAYILFPHGNVVNRFVAAVTDISNGGYGLVGG
                     TFNVAKDIIPNKFHSKIGFASAISAIEPVNGGKFMGWEVNGKLGYDLGAFLTVEAHAA
                     YMGLGSFYDSPSVNGGSQTRPVNPWLGFVCLKWLMF"
     gene            complement(2009..2554)
                     /locus_tag="PLG24_01910"
     CDS             complement(2009..2554)
                     /locus_tag="PLG24_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23666.1"
                     /translation="MKNFRFLNIFFLCLALMIASCGKEENKGTCSDGIKNQDETGIDC
                     GGVCGACLEGTQGKWFSYPVAPILASFADSISCEFKTDLTYTVNQYKDGAKVVLTGTY
                     VQTKSGVGSIYNIKLNQTSPTALTAEGILEVSGDNKTMKYEVVQTEPSLGIAPPTAAG
                     GFGSTGGGLFGTNVVQNYVRR"
     gene            complement(2583..4070)
                     /locus_tag="PLG24_01915"
     CDS             complement(2583..4070)
                     /locus_tag="PLG24_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HMZ23667.1"
                     /translation="MEKVWQHDWMSKWAIYSPDKVAVGQLDTGEVLTYGELNRNAEKL
                     GLLLYHRFGLRKGDRIAVLDEMTNNLVTLGFACQKYGYILVPINYRLAAEEVSGIFRD
                     ADPAIIFVADLFRHLIQDEFTERVKTIEPDFLTAEYTPVQLPEIREDDVLFLIYTSGT
                     TGKPKGVKYTHKMAFWNSINTSISLIINTETVTINVMPPFHTGGWNVLLMPILHHGGT
                     VYMAKKFEASTTIQALEKHRCTVFMGVPTMLSFMADDKYFDSADLSSLDYIIVGGESM
                     PIPLIERYDRKGIAIRQGYGMTEVGPNLTSLHQSDAIRKKGSIGRPNFYVMTRVINEE
                     GLDSEPNTPGELWLSGPMVTPGYWNNIEETQKAFAEDGRWFRTGDIVIRDDEEYYYIV
                     DRLKNMFISGAENVYPAEIERVLIQLPEVKECVVVGVKDEKWGEVGMALIVRNNGSLT
                     EEKLIEYCRGRLAKFKVPKYFRFMEELPKTESGKLDRKKLKTIFY"
     gene            complement(4073..5059)
                     /locus_tag="PLG24_01920"
     CDS             complement(4073..5059)
                     /locus_tag="PLG24_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HMZ23668.1"
                     /translation="MNNAKIIASGWYAPSRIVDNQYFNEILGEDVDTWLREKVQIYER
                     RWCPDEESVADLAEQAIRQAIDHAGIRPDQVDLLIIATDTPEFISPSTASVIQDRLHL
                     TNAGTFDLNTACAGFVTALDVGSKYIQADNQYNNVVVAGVYAMSKYLDLHDKKTVTLF
                     ADGAGAVVLRRCDEGEGMIGSKLTTQGQFNEWMGIYYGATKHPITREALEHNDQKLKF
                     VKKIPPEINPDTWTHMLSELLAKHGLKPTEVDHYFFTQININTIFETMDRLQVPREKA
                     ATIMHHYGYTGSACLPMAFGQWMDAGKVKKGQIVAFIGSGGGLAFGVSLYKM"
     gene            complement(5056..6537)
                     /locus_tag="PLG24_01925"
     CDS             complement(5056..6537)
                     /locus_tag="PLG24_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HMZ23669.1"
                     /translation="MDLKQATILLLAVYAAILLFLVYRGARKTRTLKDYAIGSGFPAI
                     VVAFSLAASVSSAATFIINPGFIAYYGWSAFLAMALVLPLGLYLSLIYLSKRFQHHGN
                     FTSALTLPQWAGNRFNSKVLERLLAIFSLLMITFLVLICVGLAKIISNSLGADEVSVM
                     IGVVVFVFGYMMFGGANALVYTNFVQAVLMLIVAVILIGSGYGYFEGGVGDYYDRLNT
                     IDPMLTKAFNPTSPLFRDWFEVIFCNFVVGIAIVCQPHIITKSLLLRSEKDINKYLFY
                     SVVVLIVFFSVVLAGFYGRMMFPDLKLDGSTINLDSIMSLYVVRRFSAGVGVLVVFGL
                     LAAGMSTLESLVQSLSITITQDLVRPALGKKASKVNLKVVNRFVIAFLALVTILVSYN
                     QMVNPTLSVGILAQNGVYSFFSAAFIPVLFGVFVKDAPKSSVIAASIVAIVVHFSVYY
                     LGLTPYTSGAVRNPAVASALAIVAAFLTGTTLLLIDRKKRIIE"
     gene            6803..7546
                     /gene="fabG"
                     /locus_tag="PLG24_01930"
     CDS             6803..7546
                     /gene="fabG"
                     /locus_tag="PLG24_01930"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HMZ23670.1"
                     /translation="MKRLENKIAVITGGGSGIGRATALRFADEGAHVIIWDVADKKGE
                     EAITALKDKGSNAAYYHVDTTDKEQIAKATEDVISRYGRIDILINNAGITKDSTLLKM
                     TDEQWERVIDVNLNGVYHCTRAIAPIMAQNNYGRIINASSVVGLFGNFGQTNYAATKA
                     GLIGMTQTLSKELGRKGITVNAVAPGFIATEMIQLMPEDVLAGMEAKVPVKRLGQPEE
                     VAALYAFLASDEAAYINGAVIRIDGGIVL"
     gene            complement(7591..9081)
                     /locus_tag="PLG24_01935"
     CDS             complement(7591..9081)
                     /locus_tag="PLG24_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HMZ23671.1"
                     /translation="MNKMAKNLIVLCILTVTFPLVTSAQGNVAVLSPATEIGKSGRAF
                     IYTTAFNTKLRLTRSGISEFRPKGQPLETEICVFVDPNKKFQEYIGVGGAITDASAET
                     FAALPSERQKELITAYFDREKGIGYNIIRTNINSCDFSSGSYTYVKDKDVTLESFDIG
                     HDLKFKIPMIKEAQRIIGNGMRFFASPWSPPAWMKDNNSMLHGGKLLPEYRDSWARYY
                     VRFIKDYEQQGIPVWGISIQNEPMATQRWESCIYTAEEERDFLKKHLGPVMEQEKLGD
                     KKIIVWDHNRDLIYQRAQTYMSDPEAARFIWGIGFHWYEDWSGGTPMYDNLQRVYEAF
                     PEKKLFFTEGCAESFRQDGYNNWELGEEYGRSMIRDFNNGMVGFTDWNILLDENGGPN
                     HVKNFCFAPVHGDTRTGQLIYTNAYYYIGHFSKFIEPGARRITASSTRSPLLSTAFEN
                     PDGTLVVIVMNQGDKDMDCLVWIDGAAAEANIRGRSINTILVPPAR"
     gene            complement(9100..10533)
                     /locus_tag="PLG24_01940"
     CDS             complement(9100..10533)
                     /locus_tag="PLG24_01940"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HMZ23672.1"
                     /translation="MDMRIVRQPLYVLLMLALFSFKCSKDDQPDTPVDVPVSDVQFYQ
                     TSADGSSLLQKQKQTLKFSNKTNQYPILKVDSTQQFQELDGFGYSLTGGSAIVLNTLS
                     ASDRNKILRELFSRDSSALAISCLRISIGASDLDPAPFSYDDVPEGQTDINLEKFSIE
                     PDRTNLIPVLKQILEINPEIMIMGSPWSAPPWMKTNKNFKGGSLKKEYFPVYARYFVK
                     YIQAMKAEGINIHAITVQNEPLHPGNNPSMLMEAQDQRDFVRDYLGPAFENAGITTKI
                     IIYDHNCDKPDYPISILNDATAKKYIDGSAFHLYAGEISAMSNVHEAHPDRNLYFTEQ
                     WTGKNESFASNFMWHTQHVVIGSLRNWSRMVLQWNLANDPGFGPHTDGGCSECKGALT
                     IGGTITRNPSYYILGQISAWVPPGSVRVASDNLPGLQSVAFLTPTGRKVLLVLNTTQT
                     NQSFNIEFKDRRVTTLLKAQSAGTYIW"
     gene            complement(10678..12117)
                     /locus_tag="PLG24_01945"
     CDS             complement(10678..12117)
                     /locus_tag="PLG24_01945"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HMZ23673.1"
                     /translation="MKKKIYLAIAMFSGLFLTSCDSILDKEPLSELIDKTTDTDKPFT
                     AADAEAAMAAAYAGLKTSQAELMMLDYYVNGDAQSDNSHAGADNPDNFQIDDYKISST
                     NNNVSRDWRYFYGLVALCNTVIEKAPTAADLTPARRDEMIGEASFLRGWLYFGMVQLW
                     GGVPIVTKPVPAISAENFEEVYKILYPDRKSLEEVYAQIIEDLVRAVNFAPTAYGPNK
                     MRGTKYAAHAVLAKVYATINPHDWAKVNEHCDAVIAGGFSLLPVYDHLWDGNHENSAE
                     AIFEMNCTDWNTGGNWGSSMFVGTDWKKFNTPTHNLVDAFDAAGDNVRKASSIKFQKV
                     TSWTDAYWDTNNYPFCFKMRNTAGGQNQIFLRLADIYLLKAEALTELGQLDNVSGAQY
                     YVNLVRSRVSLTPTTATTADALRTAIANERRLELAFEGHRWYDLKRTGKAVETMSALG
                     YNINENKLLWPIPQGERDKNINLTQNPGY"
     gene            complement(12154..>13313)
                     /locus_tag="PLG24_01950"
     CDS             complement(12154..>13313)
                     /locus_tag="PLG24_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ23674.1"
                     /translation="DLKLRGGWGQTGNQEGVSDFAAYGLLGFSRITPGNVFIGPGIRR
                     LTIANPELRWEKTTQTNIGFDITVLENRLTMAADVYWKKTHGLIMSIPLPSSVGVEQP
                     FIRNDGELENKGVEFMISSINFKKEKFTWNTDFNISFNKNKVSKLGLQKIYDYAKIYS
                     NGQNAIRMTEGQPMGSFYGYISEGVDPESGNIKYRDLDGNGIITPDDRTFIGQASPKF
                     IYGMTNNLSFMGFDLSIFIQGVQGNKIFNATKMDLEGMFDSKNQSIRVLDRWKNPGDI
                     TSLPRANEAGDGSLDNIHNSTRFVEDGSFLRLKAVTLAYNIPTGWLKRANIRNLSIYA
                     TAQNLLTLTKYSGFDPEVNAYGVNSRGTEFGVDYGTYPQTRQIIFGLNLGL"
BASE COUNT         3510 a         3335 c         2810 g         3658 t
ORIGIN      
        1 gatgcatcgg taatgatcct tgagcgatac tatattcttc ttccaggcag gtatatagga
       61 ggcaaggcca tggatgaaaa gtattgtttg ggggcctgaa ccgctttcag aatacgccat
      121 ttcgatatca ttgctcaggt gggcatattt gaccggcata ccatagtcca cgtctttcat
      181 cgatgacaca gcaagggatt tattcagtga tgcacaggat gacaaggtca gaaaaaggcc
      241 cagaatcagg ctgatgccaa gcgcacccct gtgattaata aatgtggtgg cgtttctgaa
      301 agtggctgct tttccaccac cagcattgct tctgatacta taaaatttat ccatcattgc
      361 catccattta aaacattaac catttaagac agacaaaacc gagccatgga ttgaccggcc
      421 tggtttggct acctccattg acagaagggg aatcgtaaaa gctacccaac cccatgtaag
      481 ccgcatgtgc ctctacggtc agaaaggcgc caagatcata tcccaacttg ccattcactt
      541 cccatcccat aaatttgcct ccattgacag gttcgatagc tgaaatagct gaagcaaagc
      601 ctatcttgga atggaatttg ttgggtatga tatccttagc cacattgaat gtacctccga
      661 caaggccata cccgccattg gagatatcag tcactgctgc cacaaaacga ttgactacat
      721 tgccatgtgg gaagagtatg tacgcaccac tgcccacaaa tatagctgcc ggacttcccc
      781 aggtattggc agtcaccaca cctgaatatt ttccatcgga tataccattg gcatcacccg
      841 tagttacaag tgcctctgcc gtaatagcat cgcccgtcgt ctgaccataa cgatagcctg
      901 cccggagatt ggctgatagg cctccgatgg agacagtctt gtcaaatttc tttgtcgatt
      961 cagcgtattc gctgatcttg cccatattat aattgaaaaa tcctgttagc aggaatcgat
     1021 ccagcatcat ttcatcattc cgggagaaat atcctcccaa ccacgtagca tccatatgat
     1081 aacgcgttga cgccaaagga aacctataag tcccattata cagagtcagc ggacttgacg
     1141 gtccctggcc aaggatggat acacctcctc ttccttccga ccggtcacgg acatggtaaa
     1201 tcgatgctcc gacattccat tttggtgaaa tcgtgtgctg gtgattgagc tcaaacatcg
     1261 tcacatcatc attgacttct acattgtttt cataaaactt gtagaagcca cctttcgtct
     1321 tggtatagtc tgtctgacga cgcacggaaa ctcccactgc atccgtaccg aaaaaggcaa
     1381 accgatagcc tgtctgcatg aatttgtcaa ccgtagttcg atacaggtca tggggggtgt
     1441 caaacatacg ctgcaatccg atattgactg tccagttctt aaccggtgcc aattctacct
     1501 ccagattctg cgtctgtata ttgacctggt cagcagaaac tgcagatcct tgatttcccc
     1561 ctgtaccata agccacatca ccccaggtcc agtcgatctc aaaagaggca cgcaacgtcg
     1621 cctttccatc aaagatccgg ggtgtatata caaagaacgg tatggccctt tgctccacat
     1681 agaatgctgt cagactgtcg gaggtgcttg tcgtattact tccgaataga cgcccgacga
     1741 tctgcccttt cagaaagtca ttctgtgggt acatattcat cgatacgccc tgggtataga
     1801 aatagacaaa ggcctgcaat tccttattgg ctttttgcgg tacttccttg tcgaatcctt
     1861 taaaacccgc tcctgtcata tcttgagcat caagattcag cggaaaccat aacaaaatag
     1921 tcaatccggc aaatatctga aaaaaccgga caattcctgt ccacgtggtg aaatcgcccg
     1981 gcttgattct tactccaatc ataagagctt atctgcgtac atagttctga actacattgg
     2041 tgccgaataa accaccacca gtactgccga atccacccgc tgctgtcgga ggagcaatcc
     2101 ccagggaagg ctcggtctga actacttcat acttcatcgt cttattgtct cctgatacct
     2161 ctaaaatacc ttcagcagta agtgccgtag gagaggtctg attcaatttg atgttgtaaa
     2221 tactaccgac acctgactta gtctgtacat aggttcctgt caggacaacc tttgcaccat
     2281 ccttgtattg attaactgta taggtaagat cggtcttgaa ttcacatgag atggaatctg
     2341 caaatgaagc aaggattgga gctaccggat aagagaacca tttgccctga gttccttcga
     2401 ggcaggcacc gcacacgcca ccgcagtcaa tacctgtctc gtcctgattc ttaatgccat
     2461 cgctgcatgt acccttgttt tcttcctttc cacaacttgc tatcattaaa gccaggcaaa
     2521 ggaaaaagat gtttagaaat ctaaaatttt tcatgacgta gattaatttt ttaaatgttt
     2581 gtttaataaa atatagtttt taattttttc ctgtccagtt tgccactctc tgtcttcggt
     2641 aattcttcca taaacctgaa atatttcggc actttgaatt ttgccagtcg gccgcgacaa
     2701 tattcaatca gcttctcctc cgtgagactt ccattattac gcacaataag tgccatgccg
     2761 acttcacccc atttttcatc ctttacaccc acaacgacgc actccttaac ctccggtaat
     2821 tgtatcaata ccctttctat ttcggccgga tagacattct ccgcacctga aataaacata
     2881 tttttcaggc gatcgacgat gtaataatac tcctcatcat cgcgaatgac gatgtcgccg
     2941 gtcctgaacc atctgccatc ctcagcaaat gccttttggg tctcctctat gttgttccaa
     3001 taaccgggtg taaccatcgg gccacttagc cacaattctc ccggggtatt tggttctgaa
     3061 tccagtcctt cttcattaat gacgcgtgtc ataacgtaaa agttaggccg tccgatactt
     3121 cctttcttgc gaatagcgtc actctggtgt agggaggtga gattgggtcc cacttctgtc
     3181 attccgtacc cttgacggat tgcaatgccc ttccggtcgt atctttcgat aagcggaatc
     3241 ggcatggact ctccaccgac aatgatatag tccagactac tgagatcggc ggagtcaaaa
     3301 tatttgtcgt ccgccatgaa ggacaacatg gttggcacgc ccatgaaaac cgtgcatcga
     3361 tgcttttcca gtgcttgaat cgttgtggaa gcttcgaact tcttcgccat atataccgtt
     3421 cctccatgat gcagaatagg catcagtaaa acattccatc ctcctgtatg aaagggtggc
     3481 atgacattga tcgttacagt ctccgtattg ataataaggc taatggaagt gttgatgctg
     3541 ttccagaaag ccatcttatg ggtgtatttg acccccttgg gcttgccggt agtgccggaa
     3601 gtgtatatca gaaacaacac atcatcctct ctgatttctg gcaactgaac cggagtatat
     3661 tccgctgtga gaaaatcagg ttcaatcgtt ttcacccttt ccgtaaattc atcctgaatc
     3721 aggtgccgga agagatctgc aacgaaaata attgccggat cggcatccct gaatataccg
     3781 ctgacttctt ctgcagccaa ccgataattg attgggacca gaatatatcc atatttctgg
     3841 catgcaaacc cgagagtaac caggttgttg gtcatttcat cgagtacagc gatccgatcg
     3901 cccttcctca atccgaaacg atgatacaac agtaatccca gtttttccgc atttcggttc
     3961 aattctccat atgtcaacac ttctccggtg tccaactgtc caacagcgac cttatccggg
     4021 ctgtatatcg cccatttact catccaatca tgttgccaca ccttttccat cgtcacattt
     4081 tatacaacga aacaccaaag gctaatccgc ctcctgaacc tataaatgct acaatctgcc
     4141 ccttttttac cttgccggca tccatccact ggccaaaggc cattggcagg caggcagagc
     4201 ctgtatatcc gtaatgatgc atgatggtcg ctgctttttc tctaggcact tgcaagcggt
     4261 ccattgtttc aaagatagtg tttatattga tttgggtaaa gaaataatgg tccacctctg
     4321 ttggtttcag tccgtgtttt gccagcaatt cactcaacat atgcgtccag gtatcaggat
     4381 tgatctccgg tggaatcttc ttgacgaact tcagtttttg gtcattatgc tccaatgctt
     4441 cccgagtgat gggatgcttt gtggcaccat agtaaatacc catccattca ttgaattggc
     4501 cctgagttgt caacttgctc ccaatcattc cttcaccctc atcacatcgt ctcaacacta
     4561 ccgccccggc gccatcggca aagagcgtaa ccgtcttttt atcatgcagg tcgaggtact
     4621 tgctcatggc atagacaccc gccactacta cattattata ttgattatcc gcctgtatgt
     4681 atttgctgcc tacgtccagc gcagtaacaa atcctgcaca tgctgtatta aggtcgaagg
     4741 tgccggcatt ggtcaggtgc aacctgtctt gaatgacgga tgccgtagat ggcgaaatga
     4801 attccggtgt atctgttgca atgataagta aatccacctg atcgggccgg atgcccgcat
     4861 ggtctatagc ctgtcggata gcttgctcgg cgaggtcagc cacactttcc tcatccggac
     4921 accatcgtct ttcatatatt tgcactttct cgcgcaacca tgtgtccaca tcctctccca
     4981 atatctcatt gaaatattga ttatcgacga ttcgggatgg cgcgtaccag ccggaggcga
     5041 ttatttttgc gttattcatt cgattattct cttttttcgg tcaattaaca acagggtcgt
     5101 ccctgttaaa aatgcggcta caatagccag ggcggaagcc actgccgggt tgcgtaccgc
     5161 tcccgatgta tatggtgtca acccaaggta atacacacta aaatgcacaa caatcgccac
     5221 aatagatgcg gcaatcacgg aagactttgg agcatccttg acaaatactc cgaacaaaac
     5281 aggaataaaa gcggctgaaa agaaggaata gactccgttc tgagccagaa tgcccacact
     5341 gagggtggga ttgaccatct gattgtaact taccaatatg gtcaccaatg ccagaaaagc
     5401 aataacaaat ctgttgacca ccttgagatt gacctttgag gctttctttc ccaatgcagg
     5461 ccggaccaga tcttgtgtga tggtgattga tagcgattgt accagcgatt caagtgtaga
     5521 cattccggcc gccaataaac caaacaccac aagtacaccc acacctgcag aaaaccgccg
     5581 cacaacatat aaagacatga tactgtccag attgattgtg ctaccgtcaa gtttgagatc
     5641 cggaaacatc attctgccat agaagccggc tagtacaacg gaaaagaaga cgatcagcac
     5701 gacgacagaa taaaaaagat acttgttgat gtccttttca gaccgcagca acaaagactt
     5761 ggtgatgata tgcggctgac atactatagc aattcctacg acgaaattgc agaaaatcac
     5821 ttcaaaccaa tcacggaaca atgggctggt tggattaaag gccttggtca gcataggatc
     5881 aatcgtattc agcctgtcgt aataatcacc tactccgcct tcaaagtagc catatccgga
     5941 cccgatcagg atgacggcca cgatgagcat gagcactgcc tgaacaaaat tagtatatac
     6001 cagagcgttg gccccgccaa acatcatata tccaaataca aaaacaacga caccgatcat
     6061 tacagagacc tcatccgctc caagggaatt gctgattatc ttggccagac ccacacagat
     6121 aagcaccagg aaagtaatca tcaacagcga aaatatggca agaagtcgct ccaacacctt
     6181 gctgttgaat ctattacccg cccattgagg cagagtcaag gcactggtga agttgccatg
     6241 atgttgaaac ctcttactca gatagatcag agacaaataa agtcccagtg gtaacaccag
     6301 ggccatggcc agaaaggccg accatccgta atacgcaatg aaccccggat tgatgataaa
     6361 tgtcgccgca ctcgaaacac tggctgcaag ggaaaaggca acgacaatag ccggaaaacc
     6421 ggatccaatg gcataatcct tcaatgtacg ggttttcctt gcaccacgat acaccaaaaa
     6481 cagtaggatg gcagcatata ctgccaaaag taagatagtc gcctgcttca gatccattct
     6541 atagatgtat tcatataaac cattgctgtc taacgcctac aaagttgaag gattcattcg
     6601 gataatcata ttgttatttg tttatatttt ggtgcgactt tttataatta tttaacttat
     6661 atttttgata tataggatta tatgattata ttttatggtg cgaccttttg gcgttttagt
     6721 ttaaataata ttcctattcc aatggatacg gccttatttt tgccgggtta atcaatttta
     6781 tttaaaaaca taattttacc taatgaagag actggaaaat aaaattgctg tcataacagg
     6841 cggcggaagt ggtatcggca gggccacggc actgcgtttt gcggatgaag gcgctcatgt
     6901 gatcatctgg gatgtcgcgg ataaaaaagg agaagaagcc atcactgcat tgaaggacaa
     6961 gggaagcaat gctgcctatt atcacgtgga cacaaccgac aaagaacaaa tagcaaaggc
     7021 gacggaagat gtcatcagcc gttatggcag aatagacatt ctaatcaaca atgcaggcat
     7081 taccaaggat tcaacgctgt tgaaaatgac cgacgagcaa tgggagcgcg tgattgacgt
     7141 caatctcaac ggcgtatatc actgcacacg agccatcgcc cccatcatgg cacaaaacaa
     7201 ttacggtcgc atcatcaatg catcgtcggt ggtcggactc tttggcaact tcggccagac
     7261 caattatgct gctaccaagg ccggtctcat cggcatgacc cagactctgt ccaaggaact
     7321 gggccgaaag ggcattacgg tcaatgccgt tgcacccgga tttattgcta ccgaaatgat
     7381 tcagttgatg ccggaagatg tcctggcggg tatggaagcc aaggtgcccg tcaaacgcct
     7441 cgggcaaccg gaggaggtgg ccgccctata cgccttcctt gcatccgatg aagcggctta
     7501 tatcaatggg gcagtgatta gaatcgacgg ggggattgtg ttgtaataaa agcatggtct
     7561 cccggcaaat tcttcaactt ttctcccggc tcatctggca ggaggaacca aaatcgtgtt
     7621 gatacttctc ccccgaatat ttgcttcggc agcagcccca tcgatccata ccaggcaatc
     7681 catatcctta tcgccctgat tcatcacaat gacaaccagg gtgccatcag gattctcaaa
     7741 ggccgtgctc agcaaggggc tgcgtgtcga tgatgcagtg atgcggcggg ctcccggttc
     7801 tatgaattta gaaaaatggc caatgtaata ataggcattg gtatatatga gttgccctgt
     7861 ccttgtatcg ccatggaccg gggcaaaaca aaaattcttt acatggttgg ggccgccatt
     7921 ttcatcaagt aaaatattcc agtctgtaaa tcccaccatg ccattgttga agtcgcgaat
     7981 catacttcgt ccgtattctt caccaagttc ccaattgtta taaccatcct ggcgaaaact
     8041 ttcggcacag ccttctgtaa agaacagttt cttttccggg aatgcctcat atactctttg
     8101 tagattgtca tacatgggtg tgccgccact ccagtcttca taccagtgga agccgatgcc
     8161 ccagataaat cttgcggcct ccggatcaga catataagtc tgcgcacgct ggtatatcag
     8221 atctcggttg tgatcccaaa caatgatttt cttgtctccc agtttttcct gttccattac
     8281 cgggcccaga tgttttttaa gaaaatctcg ttcctcttca gccgtataga tgcagctctc
     8341 ccagcgttgt gtagccatag gttcattctg tatggaaata ccccaaaccg gaattccttg
     8401 ctgctcatag tctttgataa agcgaacata atacctggcc cagctatctc tgtattccgg
     8461 aagcaacttt ccgccatgca acatactgtt gttgtccttc atccaggccg gaggtgacca
     8521 ggggctggca aaaaaacgca ttccgttgcc aattatccgc tgtgcttcct tgatcatagg
     8581 aatcttgaat ttcagatcgt gtcctatatc gaatgactcc agtgtaacat ccttgtcttt
     8641 cacataggta tagctgccac tactgaagtc acagctgttg atattcgtcc tgatgatatt
     8701 atatccgatg cctttttctc tgtcaaaata tgccgtaatc aactcttttt gcctttccga
     8761 aggcaaggcg gcaaaagtct ctgcagatgc atccgtgatg gccccaccca cccctatata
     8821 ttcctgaaat tttttgttcg gatcgacaaa aacacagatt tctgtctcca gcggctgccc
     8881 tttgggcctg aactcactta tcccggacct ggtcagcctt agctttgtgt tgaatgctgt
     8941 ggtataaata aaggcacgac ctgatttgcc gatttccgtt gcaggactta agaccgcaac
     9001 attgccctgt gctgaggtca ccagcgggaa agtcaccgtt aatatgcaca acacgattag
     9061 gttcttggcc atcttattca tatccttatt tttttattac taccaaatat aggttccggc
     9121 agactgcgcc ttcagcaatg tcgtcaccct ccggtcctta aattctatat tgaatgactg
     9181 gttagtctgt gtggtgttca gaacaagcaa cacctttctt ccggtcggtg taaggaatgc
     9241 cacgctttga agtccgggca gattatcact tgcgacacgg acactacccg gtggtaccca
     9301 ggccgaaatc tgtccaagga tataatacga cggattcctg gtgatggtcc ctccgatggt
     9361 cagtgctccc ttgcactcag aacaccctcc gtcagtatgg ggtccgaaac cgggatcgtt
     9421 ggcaaggttc cactggagta ccatccttga ccaattgcgc aacgaaccaa tgacgacatg
     9481 ctgggtatgc cacataaaat tggatgcaaa cgactcattc ttaccggtcc attgctctgt
     9541 aaagtaaagg ttcctgtccg ggtgtgcctc atgtacgtta ctcatggctg atatttcccc
     9601 ggcatacagg tgaaaagccg agccgtcgat atactttttt gctgtggcgt cattcaggat
     9661 agatatcgga tagtccggtt tgtcgcaatt gtgatcgtaa atgatgatct tcgtcgtgat
     9721 acctgcattt tcaaaagccg gaccgaggta atcccgtaca aaatctcttt ggtcttgagc
     9781 ttccatcagc atcgatgggt tgttgcccgg atgaagaggt tcgttttgga cagtaatggc
     9841 atggatattg atgccttcgg ccttcatggc ctgaatgtat ttcacaaaat accgggcgta
     9901 gaccggaaaa tattctttct taagactgcc ccccttgaaa ttcttattag tcttcatcca
     9961 tgggggagcg ctccatggtg agcccataat cattatttca gggttgattt ccaggatttg
    10021 cttcaagaca ggaataagat tggtcctgtc cggttcaata ctgaattttt ctaagttgat
    10081 atctgtctgg ccttccggta catcatcata ggaaaaaggc gccggatcca ggtcagatgc
    10141 accaatgctt atccgaaggc aagaaatggc cagggccgat gaatctctcg aaaaaagctc
    10201 ccttaatatc ttattccgat ctgaagcaga aagcgtattc aaaacaatgg cacttccccc
    10261 ggtcaggcta taaccaaaac catcaagctc ctgaaactgc tgtgtgctgt ccaccttaag
    10321 tattggatat tggttcgttt tgttcgaaaa cttcagtgtc tgcttctgct tttggagtaa
    10381 ggagcttcca tcggctgatg tttgataaaa ttggacatcg gagaccggca catctacagg
    10441 cgtgtctggt tgatcatcct tcgaacattt aaaggaaaac aatgccagca tcaaaagtac
    10501 atacaaaggc tgtcttacga ttctcatatc catcaagtct atttattgat aaaggggcgt
    10561 ggcgatattc attcttccga taaatatcgc ccacccaaaa gcaatgttat cttagcaccg
    10621 aaaatagttg ctcaccataa tctaccactt cacaattccg ggccgggaag gtatctgtca
    10681 ataacccgga ttctgggtca ggttgatgtt cttatcccgt tctccctgtg gaattggcca
    10741 caataattta ttctcattga tattatatcc aagtgcactc atcgtctcta ctgccttacc
    10801 ggtacgtttc aggtcatacc accgatggcc ttcaaaagcc agttcaagcc ttctctcgtt
    10861 ggctatggca gtacgtagtg catcggctgt tgtcgctgtt gttggtgtca gactgaccct
    10921 acttcggacc agattgacat aatattgagc accggaaaca ttatccaatt gtcccaattc
    10981 ggtcaacgcc tctgccttca aaagatatat atccgccagg cgcaggaaga tctgattttg
    11041 tccccccgcc gtattcctca tcttaaaaca gaacgggtag ttgttagtat cccagtaagc
    11101 atcggtccag gaggtgacct tctgaaactt gatgctactc gctttccgaa cgttatctcc
    11161 tgcggcatca aaagcatcca ccaggttatg cgttggggta ttgaattttt tccagtccgt
    11221 accgacaaac atggaagatc cccagttgcc acctgtattc caatcggtac agttcatttc
    11281 aaatatggcc tcggcactgt tttcgtgatt tccatcccac aggtgatcat aaaccggcaa
    11341 aaggctgaat cctccggcaa tgacagcatc gcaatgttca ttgactttag cccaatcatg
    11401 cggattgatg gtggcatata ccttggcaag aacagcatgg gccgcatact tggtcccccg
    11461 catcttgttg gggccatagg cagtcggcgc aaaattgacg gcgcgcacca ggtcttcaat
    11521 aatctgagca tacacctctt ccagagattt tcgatccggg tataatattt tatatacttc
    11581 ctcaaaattt tcggccgaaa tcgcaggaac aggcttggtc actattggca cccctcccca
    11641 caactgcacc ataccaaaat ataaccatcc tctcaagaaa gatgcctcgc caatcatttc
    11701 atcgcgcctt gccggagtaa ggtctgcggc agtcggcgcc ttttcaatga cggtattgca
    11761 cagtgccaca aggccataaa aatatctcca gtctctagaa acattgttgt tggtggaaga
    11821 aatcttatag tcgtcgatct gaaagttatc cggattgtcc gctccggcat gcgaattgtc
    11881 tgactgtgca tctccattga cataataatc aagcatcatc agctccgcct gtgaagtttt
    11941 caaccccgcg taagctgctg ccatggccgc ctcggcatcc gcggctgtaa atggcttatc
    12001 ggtatctgtc gttttgtcaa tcagctcaga aagcggttcc ttatccaata tgctgtcgca
    12061 ggaagtcaga aaaagtcccg agaacatcgc aattgctaaa tatattttct ttttcatcat
    12121 tttaaatttt ttaaggttgt tcattgtcag acatcaaaga ccaagattca atccaaatat
    12181 gatctgtcgt gtctgaggat atgttccata atccacacca aactccgtgc ccctgctatt
    12241 gaccccgtaa gcattgactt ccgggtcaaa acctgaatat ttagtcagtg tgagcaggtt
    12301 ttgagctgtg gcatagatgg aaagattgcg gatatttgcc cgcttgagcc atcctgtcgg
    12361 tatgttgtat gcaagcgtca ccgccttcag tcgaaggaac gaaccgtctt ccacaaatcg
    12421 tgtcgaatta tgtatattat ccagactgcc gtctcccgcc tcattcgctc gcggcaagga
    12481 cgtaatatct cccgggtttt tccatctgtc cagcaccctg atcgactgat tcttgctatc
    12541 aaacatccct tccaggtcca tcttggtggc attgaatatc ttattgccct ggacaccctg
    12601 aataaaaata ctcagatcaa atcccatgaa agacaagttg ttggtcatac cgtaaataaa
    12661 ctttggagaa gcttgtccaa taaaggtcct gtcatcaggt gttatgattc cgttgccatc
    12721 cagatccctg tatttgatat tgccgctttc cggatcgaca ccctctgaga tatatccata
    12781 gaaactgccc attggctgac cttccgtcat acgaatagcg ttctgaccat tgctgtatat
    12841 cttggcataa tcatatatct tctgcaagcc cagcttagag accttgttct tgttgaagga
    12901 aatgttgaag tccgtattcc atgtaaactt ctctttcttg aagtttatac tggaaatcat
    12961 gaattctacc cccttgtttt caagctcgcc gtcattgcgt ataaatggct gttctacccc
    13021 gacacttgac ggcagtggga tggacatgat caggccatgg gttttcttcc agtaaacatc
    13081 agctgccatc gtcaaccggt tttcaagaac tgttatatcg aagccgatat ttgtctgggt
    13141 cgttttttcc cagcgaagct caggattggc gatggtaagc cgccgtattc cgggaccaat
    13201 aaatacattg ccgggagtaa tcctggagaa gccgagtagt ccatatgcgg caaaatcact
    13261 taccccctct tgattgcctg tctgccccca tccgccgcgt agtttgaggt cat
//
LOCUS       DAOQQN010000029        13205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1597_length_13205_cov_5.956578, whole genome shotgun sequence.
ACCESSION   DAOQQN010000029 DAOQQN010000000
VERSION     DAOQQN010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13205
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1597_length_13205_cov_5.956578"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PLG24_01955"
     CDS             complement(<1..589)
                     /locus_tag="PLG24_01955"
                     /inference="COORDINATES: protein motif:HMM:NF020858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HMZ23675.1"
                     /translation="MKSWLEVISDSDFSIYNIPFGIAEIGGEKLIVTRLGDKVIVLDM
                     LQDAGVFAELDLPYGIFVADFLNDMIGLGKPITTSIRSAIQKYFTDEAYAQQFGLDAG
                     QYLIDYQLVKMAMPIRVGDYTDFYSSREHATNVGIMFRGPDNALMPNWLHLPVGYHGR
                     SSSIVVSGTDFKRPAGQMLDKDTGKPFYGKSHELDI"
     gene            complement(595..1551)
                     /gene="metF"
                     /locus_tag="PLG24_01960"
     CDS             complement(595..1551)
                     /gene="metF"
                     /locus_tag="PLG24_01960"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HMZ23676.1"
                     /translation="MKVTDYIHEAGGKTLISFEILPPLKGGSIQTIFKAMDPLMEFKP
                     PFIDVTYHREEFLYQMQPTGYYQKTSIRKRPGTVGICAAIMHRYGVEAVPHLICGGFN
                     REDTENALIDLHFLGIQNVLALRGDARRFEDKFSPEPDGHHYAVDLIHQITGMNNGSY
                     LDTNIEKGSRTDFCIGVAGYPEKHFESPNLELDIKHLKAKIDAGGNYIVTQMFFDNKV
                     YFDFVKLCRENGITVPIIPGLKPLTKLYQLNSIPRNFYVNLPNDLVREAQKAKTDEDL
                     KQIGIEWCIAQSRELVENNVPCLHFYTMSDAQVVKKICSKIV"
     gene            complement(2098..3435)
                     /locus_tag="PLG24_01965"
     CDS             complement(2098..3435)
                     /locus_tag="PLG24_01965"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HMZ23677.1"
                     /translation="MNIEVLNTFSDAEAFLYSSLPMFQRQGPAAYKADIGNISRLMET
                     VGNPHSCGIRMIHIAGTNGKGSVTHLTAAYLQACGYRVGVYTSPHYTDIRERIKINGT
                     LIGEDDFVICLQAFLPFIEEIKPSFFEIICAMAFLYFRDQQVDFAVIETGLGGRLDST
                     NIIFPILSVITNISPDHQSMLGDTLEKIAYEKAGIIKKNTPVVIGQKISETIQVFQDK
                     AASEDASISFAEDNWQCIYKYNDSHYTHLQIRDRISNTSATHKTALMGIYQTQNIITF
                     LEICRQLSLYNNIPFNWEYISIALKDIVKLTYYIGRWQIVSQKPLIILESAHNVAGIE
                     AVRKQLSGLEVGQVTIVFGTVSDKDVVPVLKLLPKKARYIWTKADIPRAMDEVELSEK
                     AAFIGLAGMTSPTVKDALQLALDHTSEDDLILVTGSIFVVGDAMTGLKEKGIL"
     gene            3536..4126
                     /locus_tag="PLG24_01970"
     CDS             3536..4126
                     /locus_tag="PLG24_01970"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HMZ23678.1"
                     /translation="MIIESQYFPPISVLAAFKRRGKVIIDTGDFFRKGSFRNKCYINA
                     SQGPLLLSVPLQKGKNNLQPFSDVRISYDEDWVSQHLSSLQTCYGKSAYFIYFFDEIT
                     ALLKERPVYLLDLNRNILKMIATYLIPEVTIIYSDSYVDEYLGEDKRNQILLKNYTLF
                     ESPRYLQVFEDRTGFVPNLSCLDLLFCLGKYGHDIL"
     gene            4292..4777
                     /locus_tag="PLG24_01975"
     CDS             4292..4777
                     /locus_tag="PLG24_01975"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HMZ23679.1"
                     /translation="MMKLIWILILGLGILTVGCKRDVESDEKKVEQILGDGNDYQELI
                     RNPITADENMDTVNIAVAKFDEVSHDFGDIKAGDIVTHTFHFVNAGKVPLLIKDATSS
                     CGCTVPEIPKQPIAPGEKGEILVRFNSENKSGYQSKVVTVFTNGYPSKYLLTIKTNIL
                     K"
     gene            4799..5095
                     /gene="yajC"
                     /locus_tag="PLG24_01980"
     CDS             4799..5095
                     /gene="yajC"
                     /locus_tag="PLG24_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HMZ23680.1"
                     /translation="MEGLYSPLLLLGMVAVMYFFMLRPQIKKQKEQQKFGEALAKGND
                     VVTSSGIIGRISKIEGNIVTLQVDPKTYIKVTKGSISKELTEAMGSSIQDSINN"
     gene            5102..6031
                     /locus_tag="PLG24_01985"
     CDS             5102..6031
                     /locus_tag="PLG24_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23681.1"
                     /translation="MAEKVNRDKWLFLICVIVAIVFWAMQKLSNNYSFTQVYSVSYII
                     PDGKVFKKPPVTSVEVTLTGIGWDIVNKSAKNIGDKIEVELSADNSQLIPATAISEKI
                     NSLLASDEISVAQIKPSNISISLVDQAKKKVPVVSKLNIEFLPSFYIKSPIHFTPDSV
                     VITGSYQAIKNITKWNTEILNINKLNRNIYDMPIQLEAPKTDLIRLDHDMVRMNLEVE
                     EFTEKSFTLPIKIKNADNKVISIPNTVDVKCVVGISKYDILTEDDIELYINLDESLVS
                     NSINVVPISVSRMPFYVRNISYQPSSAQFFYLK"
     gene            6257..7486
                     /locus_tag="PLG24_01990"
     CDS             6257..7486
                     /locus_tag="PLG24_01990"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006156.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HMZ23682.1"
                     /translation="MEKIKVANPVVELDGDEMTRIIWKFIKDKLILPYLDIDIKYYDL
                     GVEYRDATDDMVTIEAANAIKQYGVGIKCATITPDEARVKEFNLKQMWKSPNGTIRNI
                     LDGTVFREPIVMSNIPRLVPNWTAPICIGRHAFGDQYKATDFVTKGKGKLTVKFEGED
                     GSVQEFEVYNFKGDGVALTMYNTDESIAGFARACFNMSLNKGWPLYLSTKNTILKKYD
                     GRFKDIFQEIFDNEFKARFDELGITYEHRLIDDMVASALKWHGNFVWACKNYDGDVQS
                     DTVAQGFGSLGLMTSVLVTPDGKTMEAEAAHGTVTRHYREHQKGNPTSTNPIASIYAW
                     TRGLAFRGKLDDNQALIHFAETLEAVCVETVESGKMTKDLAVCIHGNKVNHGEHYLYT
                     EEFLDALDQNLKAKLGQ"
     gene            7713..8594
                     /locus_tag="PLG24_01995"
     CDS             7713..8594
                     /locus_tag="PLG24_01995"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HMZ23683.1"
                     /translation="MARKNRIGIPKPKPNYFLSIVSIALVLFLLGLMALLSLNTNKLI
                     NSFKENVNLIAELRDSVPSGKIDTLKEFISSLEAVKASTVDIVTKEEALSIMKKEMGD
                     DFLLDEMTNPLNDAIVFNVKADYLDSLQLAGIKTTILNAYDFVTNVYYQETFVGKVTE
                     TLSKIGIGILVISILFLLIALTVMHGTLKLSMYSNRFLIKNMQLVGASKGFIQRPFLW
                     KGVWSGLLSALVACLMLSGVIWAAYLYIPDVSGIIDLNEVGILFGAILILGILITVIS
                     TFFIINRYIRLRREDMF"
     gene            8641..8988
                     /locus_tag="PLG24_02000"
     CDS             8641..8988
                     /locus_tag="PLG24_02000"
                     /inference="COORDINATES: protein motif:HMM:NF022741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HMZ23684.1"
                     /translation="MSQKKKSQASSGQPLKPTASTINQESTERKEATIKQVDKGNLVF
                     GKDNFKWMFIGMGVMLLGYILMSGGTMPNPETWDPSLIYSFRRITLAPFIILLGLAIE
                     VYAIFYIPKKKTS"
     gene            9035..9859
                     /locus_tag="PLG24_02005"
     CDS             9035..9859
                     /locus_tag="PLG24_02005"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HMZ23685.1"
                     /translation="MGILQAIIIAIVEGLTEYLPISSTAHMGFVAALMGMEESEYLKM
                     FQISIQFGAIQAVVVLYWKKFFDFSNLTFYTKLIIAVIPALVLGFLFDDQIEAVLGNQ
                     IAISSVLILGGVVLIFIDRWFTNPKYTDEKQISTKTAFLIGFWQCLAMMPGTSRSAAS
                     IIGGMVQGLSRKAAAEFSFFLAVPTMAAATTYSVFLKTWGKGTSNAMKGYTMILQDNE
                     HIILFLIGNVVAFLVAIAAIKFFISVLTRYGFKMWGWYRIIIGLLLLIYFYSIPQR"
     gene            9862..10914
                     /locus_tag="PLG24_02010"
     CDS             9862..10914
                     /locus_tag="PLG24_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ23686.1"
                     /translation="MWIYAGKRMMYGLMIIITVVCTIASIIYLSPVDPARLSFGQMSD
                     VGTVEAKRKAMGLDQPLPIQLMYYLRDVSPVNVIKLSNPALENYSYITIFKAGSRKLI
                     LKWPYLRESYQSGRPVSDILKEAIPLTLILAICSILLASFLGIALGILAAIRQGTWID
                     QVAIFVSTIGFSLPSYATAIFFAILFAFILKPLTGLNLTGSLLEIDNHGNWEIVLKNL
                     ILPVFALGLRPVSIITQLTRSAMLDNMSMPYVRTAKAKGLGVQKITYLHVLKNALNPI
                     ITGISGWFASLVTGAFFVENVFNYRGLGLVTVKALLDFDIPVVLGAVIFTCIMFVVIN
                     ILVDLMYVLTDPRIKY"
     gene            complement(10943..11659)
                     /locus_tag="PLG24_02015"
     CDS             complement(10943..11659)
                     /locus_tag="PLG24_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HMZ23687.1"
                     /translation="MRYCLSIIVAMLLFVHVADAQRRNNNDYQSSNYRDFVSKPYFFG
                     LTLGYNQTSYKVFRSGDFLVSDSIVGINSGVGQGFIVSVISNLKVGEYIDFRLMPTFS
                     FTERKLRFDLINSSSPYLDRKAESVFVELPFQVRYKTDPYKDIRAFVLGGVKYSFDVS
                     NKNRALEAQNILKIAPTDFAIEIGAGLQFFLPFFILSPEIKYSHGINNILIYNRQKET
                     SKVLDKIFSRTWTISFHFEG"
     gene            complement(11656..12381)
                     /gene="ubiE"
                     /locus_tag="PLG24_02020"
     CDS             complement(11656..12381)
                     /gene="ubiE"
                     /locus_tag="PLG24_02020"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HMZ23688.1"
                     /translation="MSEVKPYSAEEAKKEQIEQMFDNIAHSYDFLNHFFTLGIDRGWR
                     RKMIRMIAGSNPKKILDVATGTGDVAIMMAKMLKPEKITGLDLSAGMLAIGKEKIKKA
                     ALENTITLQQGDSEKLPFEEGAFDAVTVAFGVRNFEDLRKGLSEMHRVTRVGGEVAIL
                     EFSMPHNRIFNFLYKIYTKYLMPVIGKFTSGDKAAYSYLFESVQQFPHGQTFINILRE
                     VGFRESVARPLFGGVCTIYKSIK"
     gene            complement(12430..12978)
                     /locus_tag="PLG24_02025"
     CDS             complement(12430..12978)
                     /locus_tag="PLG24_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HMZ23689.1"
                     /translation="MKMNKIVAIIILYFFNVITMDAQSIYDFKVESLTGGQLNLADYK
                     EKKMLIVNTASQCGYTPQYAGLEKLYNVMKDHDFVIIGFPSNEFGKQEPGSNDEIASF
                     CQINYGVTFPMAAKMVVKGDGISPLFEFLTKQAIDKGIEDPIKWNFTKFLIDEQGNLV
                     GVFPSKVDPMSEELQNAILSPK"
     gene            complement(12990..>13205)
                     /locus_tag="PLG24_02030"
     CDS             complement(12990..>13205)
                     /locus_tag="PLG24_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23690.1"
                     /translation="KVRNNWVSDTLNLAEFIDIKGFKAVGNKLSSEKLSNFSAAPQPE
                     AKSIDNEQHNIGDTIEFDIETGQTSLF"
BASE COUNT         4010 a         2721 c         2578 g         3896 t
ORIGIN      
        1 cgatatccaa ttcatgactc tttccataaa acggttttcc ggtatccttg tccagcatct
       61 ggccggctgg gcgcttaaaa tcagttcccg aaacaacaat ggatgatgac cgaccatgat
      121 agccgacagg aagatggagc caattgggca tcagggcatt gtcaggcccc ctgaacataa
      181 tgccgacatt ggtagcatgt tcccgactag aataaaagtc ggtatagtca cccactctaa
      241 ttggcatagc catctttacc aattggtaat caatcagata ttgaccagca tcaagtccga
      301 attgctgtgc ataggcctca tccgtaaaat atttttgaat cgctgatctg atagatgttg
      361 tgattggctt gcctaagccg atcatatcat tgagaaaatc agctacaaag atcccatagg
      421 gcagatccag ctcagcaaac acaccggcat cctgaagcat atcaagtaca atgaccttat
      481 cacctagcct ggttacaatc agcttctctc cgccgatctc agcgatgcca aaagggatgt
      541 tataaatact gaaatcagaa tctgatatga cttccaacca acttttcata ttccttatac
      601 tattttagaa caaatctttt tgactacctg agcatcactc attgtgtaaa aatgaagaca
      661 tggtacatta ttctcaacca actccctgga ctgagcaata caccattcga tgccaatttg
      721 cttcaagtcc tcatccgttt tcgctttttg agcttccctg acaagatcgt tgggcagatt
      781 gacataaaag ttccttggga tagaattcag ttgataaagc ttagtcaaag gtttcagacc
      841 cggaatgatg ggaacagtta ttccattttc acgacacaat ttcacaaaat cgaagtacac
      901 cttattgtca aaaaacattt gtgtgacaat ataattacct ccggcatcga tcttagcttt
      961 taaatgtttt atatccaatt cgaggttcgg ggattcaaag tgcttctcag gatatccggc
     1021 tacgcctata caaaaatctg ttctgcttcc tttttcgata ttggtatcaa gataacttcc
     1081 attgttcata ccagtaattt ggtgaatcag atctactgca taatgatgtc catcaggctc
     1141 aggagagaac ttatcttcaa atcgccgggc atcgccacgg agggccaata cattctggat
     1201 acccagaaaa tgcagatcga tcagtgcgtt ctcggtatct tccctgttaa agcctccaca
     1261 gattaagtgg ggtactgcct ccactccata acgatgcatt atcgccgcac aaataccaac
     1321 cgtaccgggc cgtttacgta tggatgtttt ctggtaataa cctgtaggct gcatctggta
     1381 taagaactct tcccgatgat aggtaacatc aataaagggg ggcttaaact ccatcaaagg
     1441 gtccatagcc ttgaaaattg tctgaatact tccccctttc aaaggcggca gaatctcgaa
     1501 ggaaatcagg gtcttacccc cggcttcatg gatataatcg gtaactttca tttacttatt
     1561 ccaaatatga ggcaaaggta agtcatgttt agaaaaatat agaacgtatt tccaacacat
     1621 tataaatatt tataatatta tatcatgcca aatatataat cgataatgac tattttattt
     1681 gcgatggcca ataatagcag cctttataag ccgaaaacat atctattaca aaattgtagt
     1741 aaaatccttc aggctaccac attttcctcc ggacttccca cgttgtgaat atatccctca
     1801 gaaaacatat gttggcactt cctatggtgc gtatcccctt taaatacatg gtaccggcaa
     1861 catgtccaca tcgagattat tttctttgat gggtgcgtat ccccttaaaa tacatggtac
     1921 cggcaacata gattcacaat caggcttaaa caagctatga ttgataatca aaaactttca
     1981 tgattctctt atcatccact attcggaatt gcctcaggtt ttaataaaaa tgaaatggca
     2041 atcaatccat cgattgttga ccggaagata accttttcag ctatttctct gtcagaatca
     2101 caaaattccc ttttctttca accctgtcat ggcatcgccc acaacaaata tggagccggt
     2161 aacaagaatc aaatcatcct ccgatgtatg gtctaaggca agttgtaggg catctttaac
     2221 ggtgggacta gtcatacctg caagaccgat aaatgcagct ttctccgaca attcgacttc
     2281 gtccatagct cgagggatat ccgcttttgt ccagatatac cgggcctttt taggcaacaa
     2341 tttgagtaca ggtactacat ccttatccga aacagttcca aagacgatgg ttacctgacc
     2401 cacctccagt ccactcaatt gtttccggac tgcttcaatt cctgcaacat tatgggcgct
     2461 ttcgaggatg atgagcggct tttggctaac aatctgccac cttccgatat aataagtcag
     2521 cttcacaatg tcctttaaag ctattgatat atactcccaa ttaaagggaa tattattata
     2581 aagactgagt tggcggcata tctcgagaaa tgttataata ttctgagtct ggtaaatgcc
     2641 catcaaggca gttttatgcg tggcggaagt gttggaaatt ctatccctga tttgaaggtg
     2701 ggtataatgg ctatcgttgt atttatatat acactgccaa ttatcctccg caaaagaaat
     2761 cgaagcgtct tctgatgcag ctttgtcttg aaagacttga attgtctccg atattttttg
     2821 tccaataacc accggagtat ttttcttaat gattcctgcc ttttcataag cgatcttctc
     2881 aagcgtgtct ccaagcatgg attgatgatc gggactaata ttcgtaataa cactcaatat
     2941 tggaaaaata atattggtac tatccagtct gccacccaga ccggtttcaa ttacagcaaa
     3001 atccacctgt tgatcccgga aatacaggaa ggccatcgca cagatgattt caaaaaagga
     3061 gggttttatt tcttcaatga atggtaaaaa tgcttgaagg catatgacaa aatcatcttc
     3121 accaatcagc gtaccattta ttttaattct ttcccggata tcggtataat gaggtgaagt
     3181 atagacaccc accctataac cgcacgcttg caggtaggca gcagtcaggt gggtaacact
     3241 acccttgcca ttagtcccgg ctatatggat cattctgatg ccacaggaat gcggattacc
     3301 cacagtctcc atcaatctgg aaatattgcc gatatcggcc ttgtaagcgg cagggccctg
     3361 ccgttggaac atcgggagac tactatataa aaaagcttca gcgtcggaga aagtatttaa
     3421 cacctcaata ttcatttgac caattataag atgtaaaggt atattttatg aaacataggt
     3481 tagcctcaaa aatataattt aaagcgctta ttatctttga gaccaaaaaa ggaagatgat
     3541 catcgagtcc cagtactttc cacccatttc cgtccttgct gccttcaagc gccggggaaa
     3601 ggtaataatt gacacgggtg attttttcag gaaaggaagc ttcagaaaca aatgttacat
     3661 caatgcttca caagggccct tgttactttc agtaccactt cagaaaggga aaaacaacct
     3721 gcaaccattt tcagatgtca ggatcagcta tgacgaagat tgggtaagtc aacatctatc
     3781 ctccttacaa acctgctatg gcaagtccgc ttattttata tatttctttg acgaaatcac
     3841 cgcactttta aaagaaagac cggtgtatct tctcgatctg aatcggaata ttctgaaaat
     3901 gattgcaacc tacctaattc cggaggtaac cattatatat tccgactcgt atgtagatga
     3961 atatttgggg gaagataaaa ggaatcaaat ccttttaaaa aattataccc tcttcgaaag
     4021 tccgcgatac ttgcaggtat ttgaagatag aacaggcttc gtgccaaatt taagctgcct
     4081 tgatctttta ttctgtcttg gaaagtatgg tcatgacatc ctataaccag gactatttta
     4141 ttcaaactct cattcctccc gataatcaac tgattattag ataagtatat ttgtaaaatc
     4201 aaaaaataag ttccaacatt taaattttac tacctttggg tcgttgtaat aaataaaaaa
     4261 atcatatatt ttaaacaaca acattcaagt tatgatgaag ctgatttgga tccttatttt
     4321 gggcttaggc atactaacag taggttgcaa gcgggatgtt gaatccgatg aaaagaaggt
     4381 agagcaaatt ctcggtgatg gtaatgacta tcaggaattg atacgcaatc cgattactgc
     4441 cgatgagaat atggacacgg tgaatatagc agtagccaaa tttgatgagg tcagccatga
     4501 ttttggagat atcaaggcag gagacatcgt gacacataca tttcattttg tcaacgccgg
     4561 aaaagtgcct cttcttataa aagatgcaac ttccagttgt ggatgtaccg ttccggaaat
     4621 ccccaaacaa ccgattgcac cgggtgaaaa gggcgaaatt ctggtgagat ttaacagtga
     4681 aaacaaatcc ggctatcagt caaaggtcgt aaccgtattt accaatggat atcccagcaa
     4741 atatcttctg acgataaaaa ccaatattct taaataaaac aaaaataatc aattacttat
     4801 ggaaggtcta tattctccat tattattact tggaatggtc gcagtaatgt attttttcat
     4861 gttgaggcct caaattaaaa aacaaaaaga gcagcaaaaa ttcggagaag cacttgcgaa
     4921 aggcaatgat gtcgtcacat caagtggaat catcggtcga atcagcaaga tagagggaaa
     4981 catcgtaacc ctacaggttg atccaaaaac atatataaag gtaacgaaag gttccatctc
     5041 aaaagagctt accgaggcta tgggaagttc aattcaagat tcaatcaata attgataaac
     5101 aatggcagaa aaggtcaacc gggataagtg gctttttctc atttgtgtga ttgtagccat
     5161 tgttttctgg gcaatgcaaa aattgtccaa caattatagt tttacacaag tgtattccgt
     5221 cagttacatc attcctgacg gcaaggtatt caagaagcca cccgtcactt cggttgaagt
     5281 tacattaacc ggaataggtt gggatattgt caacaaatca gctaaaaata tcggtgataa
     5341 gattgaagtt gagttgagtg ccgataacag ccagcttatt ccggctactg ccatttcaga
     5401 gaaaatcaat tcattacttg cctccgatga gatatcagta gctcaaatca agccctccaa
     5461 tatttccatc tcactggtag atcaagctaa aaagaaagta cctgttgttt caaaattgaa
     5521 tattgaattt cttcccagtt tttatatcaa aagtccaata catttcactc cggattcagt
     5581 ggttatcaca ggttcttacc aagccataaa gaacatcacc aaatggaata ctgaaatctt
     5641 aaatatcaat aaattaaacc gaaatattta cgatatgccc atccaattgg aagctccaaa
     5701 gaccgacctg atccgactgg accatgacat ggtaaggatg aatcttgaag tggaagaatt
     5761 caccgaaaag agctttaccc taccaatcaa aatcaagaat gcggataata aggtgatctc
     5821 catacctaat acagtggacg taaaatgtgt agtcggaatc agcaaatatg acatcttgac
     5881 ggaagatgac atcgagttgt acatcaatct ggatgaaagc ctggtttcca attcaatcaa
     5941 tgtagtcccc atcagcgtat cgagaatgcc gttctatgtc aggaatattt cctatcaacc
     6001 ttcttcggca caatttttct atctgaaata atttggacct catttcaggt cttatagcat
     6061 ggctaaagat attaaattaa aattaatcat cctttatttg tttattactt tcttacatgt
     6121 cgtgcagctt ttctgaatat aattctcaaa agctattgga atattaaaat tatccttgat
     6181 tctcctaccc tttcgcattg tttttatatt tttgtgctct gaaatcaata tttgttcaac
     6241 ataaaaatca ttagtattgg aaaaaattaa agtggccaat ccggttgtgg aactggatgg
     6301 cgatgagatg acacgcatta tatggaagtt tattaaggac aagcttatcc ttccctacct
     6361 tgacatcgat atcaaatatt atgatctggg agtcgagtac agggatgcca cagacgatat
     6421 ggtgacaatt gaagctgcca atgccatcaa gcaatatggc gtaggtatca aatgtgcgac
     6481 aattactccg gatgaagcac gggtaaagga atttaacctt aaacaaatgt ggaaatcacc
     6541 caatggtact atccgaaata tcctggacgg taccgtattc cgggagccga ttgtcatgag
     6601 taacattccg cgtcttgtcc ctaactggac tgcaccgatt tgtatcggtc gtcatgcctt
     6661 tggtgatcaa tataaggcga cagactttgt aaccaaagga aaaggcaaat tgaccgtaaa
     6721 attcgagggc gaagatggta gtgtacaaga atttgaagtt tataatttca aaggagatgg
     6781 cgttgcacta accatgtata atacagatga aagtattgca ggttttgcgc gtgcctgttt
     6841 caatatgtcg ttgaataaag gttggccgtt gtatttgtct accaaaaata caatcctgaa
     6901 gaaatacgat ggcagattca aggatatttt tcaggaaatc tttgacaatg aattcaaagc
     6961 tcgctttgac gaattgggca tcacctatga gcatcgtttg attgatgaca tggtggcatc
     7021 tgccttaaaa tggcatggca acttcgtatg ggcatgtaag aattacgatg gggacgtaca
     7081 gagtgacaca gtagcgcagg gatttggctc acttggtctt atgacatcag tacttgtaac
     7141 cccggacgga aagaccatgg aagcagaagc agctcatggg actgtaaccc gtcattacag
     7201 agagcatcag aaaggcaacc ctacatcaac caatccaatt gcctccatct acgcctggac
     7261 acgaggcctt gcatttcgtg gcaaactgga tgataatcag gctttaatac atttcgcaga
     7321 aacactggaa gcagtttgtg ttgaaactgt tgaaagcgga aagatgacca aagaccttgc
     7381 agtgtgtata catggcaaca aggtcaatca tggcgaacat tatctgtata ctgaagaatt
     7441 cctggatgca ttggatcaga acctaaaggc taaactgggc caataatcat caaataatta
     7501 gtaaaacaaa agccgttgtg atattttaca acggcttttt tgttgattcc tgtaacaaga
     7561 aaatataatc cattaggata cgaattgaag agattcaata acttacttca ggtaaaaaat
     7621 aaaatttaaa attatatatt ataaagaaat tatatatttg cacattatat atgtaaaatc
     7681 atacagattg aaccataaaa ccgtaaaaac ttatggccag aaagaataga atcggcatcc
     7741 cgaagcccaa acctaactat ttcttgtcca tagtgagtat agcgcttgtt ctttttctgt
     7801 tgggattgat ggcattactg tcgttgaata ccaacaaact aatcaattca ttcaaggaaa
     7861 atgtcaatct gattgcggag cttagggatt cggtaccttc aggaaaaatt gacacactga
     7921 aggaattcat ctcatccctg gaagcagtta aggcatccac cgtagatatt gttactaaag
     7981 aggaagctct ttccatcatg aaaaaggaaa tgggtgatga tttccttctt gacgaaatga
     8041 ccaatcctct taacgatgca atagtattca atgtcaaggc agattatctg gattcactcc
     8101 aacttgcagg gatcaaaacc accatcctta atgcgtatga ctttgtaacc aatgtctatt
     8161 accaggaaac ctttgttggc aaagtcacgg aaacattaag caagatcgga atcggcattc
     8221 tggttatatc gatactgttc cttttaattg cgttgactgt tatgcatggg acattgaagt
     8281 tgtcgatgta ttccaaccga tttttaataa aaaatatgca attggtgggt gcgagtaaag
     8341 gatttatcca gcgaccattt ctgtggaaag gtgtatggag tggcttgttg agtgcccttg
     8401 ttgcctgttt aatgcttagc ggggtgatct gggcagccta tttgtatatc ccggatgtta
     8461 gtggaatcat tgatttgaat gaagttggca tcctgtttgg ggcgatattg atactcggca
     8521 tcttaatcac agttatatcc acatttttca taatcaatcg ttatattcgt ctaagaaggg
     8581 aagacatgtt ttgataattg ggcaatacat tctacctttg aaccattaat ttttgaatat
     8641 atgagccaaa agaaaaaaag tcaggcatct tcaggtcaac cattaaagcc tactgcatcc
     8701 actataaatc aagagtccac agagaggaag gaagccacta ttaaacaagt tgacaaaggc
     8761 aatttggtat tcggaaagga taatttcaaa tggatgttta taggcatggg tgtgatgttg
     8821 ttagggtata ttttgatgtc cgggggaact atgcccaacc ctgaaacatg ggatccttcc
     8881 cttatataca gtttcaggag gattacatta gcgccgttta tcatactgtt aggtcttgcc
     8941 atagaagtct atgccatttt ttacattcct aagaaaaaaa catcctaacg tccttcattg
     9001 tcccaatatt taatctttat acactgacaa ccatatgggc attctacagg caatcatcat
     9061 cgctatagtc gaaggcctga cggagtatct cccaatttct tccactgcgc acatgggttt
     9121 tgtagctgca ttgatgggta tggaagagtc agaatattta aagatgttcc aaatttcgat
     9181 tcaatttgga gcgatacaag ctgtcgttgt tctatattgg aaaaaattct tcgacttcag
     9241 taacctaact ttttacacca aattaataat cgcggttatt cctgctttag tgctgggttt
     9301 tctttttgat gaccaaatag aggcggttct cggtaatcaa atagctattt cctcagtact
     9361 tattctgggc ggtgtcgttt tgatcttcat agatcgttgg tttaccaatc caaaatacac
     9421 agatgagaag cagatttcca ctaaaactgc ctttttaatc ggattttggc aatgccttgc
     9481 catgatgccc ggaaccagca gatctgctgc gtcaatcatt ggaggcatgg tacaaggtct
     9541 ttcaagaaag gcagcagctg aattttcttt tttcctggcg gtgcctacta tggcagcggc
     9601 tacaacctat tcggtatttt taaagacatg gggtaaaggt acctcgaacg caatgaaggg
     9661 ctataccatg atattgcagg acaatgaaca catcatattg ttcctgatag gtaacgtggt
     9721 agcttttctt gtggcaattg cggctattaa gttctttatc tctgtcttga caaggtatgg
     9781 attcaaaatg tggggctggt acagaatcat tattgggttg ctgcttttga tctatttcta
     9841 ctcgatacca caaagataag tatgtggatc tacgcaggaa agaggatgat gtatggcctg
     9901 atgatcataa tcacggtcgt atgcaccatt gcaagcatca tttatctttc accggtggat
     9961 cctgcaaggt taagttttgg ccagatgtct gatgtcggta ccgtagaggc aaaacgcaag
    10021 gccatgggcc tggaccagcc gcttcccatc cagttaatgt attacttgcg ggatgtatcc
    10081 cctgtcaatg tgataaaact atccaatccg gcattagaaa attattctta catcacaatt
    10141 ttcaaggccg gaagccgaaa gctaatcctg aaatggccct atctccgcga atcctatcaa
    10201 agtggccgac ccgtatcgga cattttgaaa gaagcaatac cattaaccct tatcctggca
    10261 atctgttcca ttcttctggc ctccttcctg ggtatagccc ttggaatcct ggcagccatc
    10321 agacaaggga catggataga ccaggttgcc atttttgtat ccactatcgg gttttcctta
    10381 ccatcttatg ccacagctat attctttgcg atcctttttg cttttatcct caaaccattg
    10441 acgggcctta atttgacagg ttcgcttctc gaaatcgaca accatgggaa ttgggaaatt
    10501 gtattaaaaa atttaattct acccgtgttt gctttaggat tgcggcctgt atccatcatt
    10561 acccaactta ccagatcggc catgctcgac aatatgtcca tgccatacgt cagaacagcc
    10621 aaagcaaagg ggctcggtgt tcaaaaaatc acctacctac atgtcctgaa aaacgcctta
    10681 aacccaatca ttacaggcat cagtggttgg tttgcatctt tagtgaccgg tgcatttttt
    10741 gtcgaaaatg tgttcaacta ccggggattg ggattagtga ccgtaaaggc tttgctggat
    10801 ttcgatattc cggtggtgct gggtgccgtg atattcacct gcatcatgtt cgttgtaata
    10861 aatattttgg tggatcttat gtacgtattg acagatccaa ggataaaata ttagaaaatt
    10921 ctgataaaat accggaaagc aattatccct caaaatggaa ggaaattgtc catgtcctgg
    10981 agaatatctt gtcaagcacc ttggatgtct ccttctgcct gttgtagatc aaaatattgt
    11041 tgattccgtg actgtacttg atttccggag ataaaatgaa gaacggaagg aaaaactgga
    11101 gtcctgcccc aatctcaata gcaaaatccg ttggcgctat cttcaatata ttttgtgcct
    11161 ccaatgctct gttcttgttg gatacatcaa aggagtattt gactccaccc agcacaaaag
    11221 cccggatatc cttataagga tcggttttgt atctgacctg aaaaggcaat tcaacaaaca
    11281 cactttcagc ctttctgtcc agataaggtg aagaagaatt gatcaaatca aatcttaatt
    11341 tacgttcagt aaatgaaaaa gtgggcatca ggcggaagtc tatatattca cctactttta
    11401 ggttggaaat aacagacaca ataaaaccct ggccaacacc ggaatttatg cctactatcg
    11461 aatctgaaac caggaaatct ccagacctga agaccttata gctggtttga ttatacccaa
    11521 gtgtaagtcc gaagaaatat ggcttagata cgaagtcacg ataattggaa ctctggtagt
    11581 cattattgtt tctccgctga gcatcagcga catgaacaaa caacaacatt gcaacaatta
    11641 tggataggca atatctcatt taatactttt gtaaattgta catactccac caaacaaagg
    11701 tctggcaaca ctttcccgga agccaacttc ccggaggata tttataaacg tctgtccatg
    11761 gggaaattgt tgtaccgatt caaataaata tgaatatgcc gctttgtctc cggaggtaaa
    11821 tttaccaatg acaggcatca ggtactttgt atagatttta tataagaagt tgaatattct
    11881 gttgtggggc atagaaaatt ccaggatggc aacttctccg cctactctgg tgacacgatg
    11941 catctctgag aggccttttc tcagatcttc aaaatttctg acgccgaaag cgaccgtcac
    12001 cgcgtcgaat gcgccttctt cgaaaggcag tttttcacta tcgccctgct gtaaggtaat
    12061 cgtattttcc aaggctgctt ttttgatctt ctccttccct atcgcaagca ttccggcact
    12121 cagatccagg cctgtaattt tctccggttt aagcatttta gccatcatga tggcaacatc
    12181 accggttcct gtagctacat caaggatttt ctttggatta gaaccggcaa tcattctgat
    12241 cattttcctg cgccaaccac gatcaatacc caatgtaaaa aaatgattca gaaagtcata
    12301 gctgtgagca atgttgtcaa acatttgctc aatctgctcc ttctttgcct cctctgctga
    12361 atatggtttt acttccgaca aagctttgat tttggcgcaa atatacaatt tacaaaggaa
    12421 tatgttttcc tatttcggtg aaagaatggc gttttgcaat tcttcactca ttggatccac
    12481 ttttgatggg aaaacaccca ctaaattgcc ttgttcatca ataagaaact tggtaaagtt
    12541 ccatttgatt ggatcttcta tccccttgtc aatggcctgt ttcgtcagaa actcaaacaa
    12601 agggctgatg ccgtcccctt tgactaccat tttagcagcc ataggaaagg taacaccgta
    12661 attgatttga cagaatgaag cgatctcgtc attgcttccc ggctcctgct ttccaaattc
    12721 attggacggg aaaccgataa tcacaaagtc atgatccttc attacattat aaagtttttc
    12781 caagccggca tactgaggcg tgtaaccaca ttgggacgct gtattgacaa tgagcatttt
    12841 cttctctttg taatccgcaa gattcaattg gccgcctgtc agggattcca ccttaaaatc
    12901 ataaatagat tgagcatcca tagttataac attgaaaaaa tataaaataa taatggcaac
    12961 aattttgttc atcttcatat cgtcaccaat taaaacaggg aagtctggcc ggtttcaata
    13021 tcgaattcga tggtatcccc gatattatgc tgttcattat caatcgattt cgcctccggt
    13081 tgcggtgctg cgctgaaatt gctcagtttt tcggagctaa gtttgttccc gaccgctttg
    13141 aagcctttga tgtcgataaa ctcagccaga ttgagggtat cacttaccca gttgttcctg
    13201 acttt
//
LOCUS       DAOQQN010000030        13009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1639_length_13009_cov_5.963332, whole genome shotgun sequence.
ACCESSION   DAOQQN010000030 DAOQQN010000000
VERSION     DAOQQN010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13009
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1639_length_13009_cov_5.963332"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3892)
                     /locus_tag="PLG24_02035"
     CDS             complement(<1..3892)
                     /locus_tag="PLG24_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23691.1"
                     /translation="MIRDPKYIGTTQSTRLENRTNPLEIAIDDRPIENLLSILYEYAS
                     LLHFKESNSDDSKDWQAFFNYDLTSILALSITKDLAQAQFDFNLKVKEFSRATDGNIQ
                     IEALEAMISVVNSLLSEFNNLRDRLLKIQYPDGEIEKFVYFDIEAYYEQSVLDYIYEL
                     HAIILGAIPHAEKQKSAFNLGYQIIESSPEDVSQEDIPNKLESVFIRLRILFNNLLET
                     SLAIRDLLIPYFVRSLSQKDDHQPHIGLLLGFLEQYKKLQTSINKVPENYLHFYYIDF
                     LKQKKRKHIAPYAYVSFAISPYVENKTIPENTILLASLDKDGLPHRFSTKYPVNVGRY
                     TLSDIKTLYFDRGRRETLTNKYSIITESFMAPYANSQDGKGSPFNSDKLPAWPTFGEG
                     QIEKEPSLRNMIESELGFSVASSVLMLGEGKRSIKIRLNFTEESVTEIVRLFKDIASK
                     EHKKSTMLEVFYKIFSTKSKKRNITILLTSAKGWHKVDPQTILIFQDGEEEWDPRHIC
                     ISFNLSPMAPPIIPFNEHLHSGKYISGSQPILMLLLNNDREPYLYSFLQKLVIETIEI
                     DARVENVRNLSVYNEYGLIDNSQPFYPFGPTPVKNSYFLLGNREIFSKKLDEFELNID
                     WFDLPETAGQFNQKYEVYERRYDEFKVQIGNLNDGQFVMEDNSRPLFMTKEIIGSDKL
                     SDEGLFTKYTLDSGDLKSLGFNSSESMEIADQFSTNANVGFLKFVLDEPEDGFGQQKY
                     QEILAKQMLKNARLINKAFVEDENEDSPELGFPEMPLIPMVSNIHINYKASDVIELTQ
                     DAVNFQEINHIHPFGYEKIYPNIKYRDRLPYLLPQYEEDGYLLLGLKNCKKAEIISLL
                     FQMTPVYYQDRDQYILPIIRWQYLTYNNNWKLLQGENILRDETDSFTKSGVIQIKIPS
                     DISTMSQIMEPNKFWLRVSATGDIQLLNHTLAIYPNATLTRWVDQSDPSGLNTPFPPE
                     SIIALDNKIHGVTSMIQPYESFGGKAEESNIEFYTRISERLRHKNRGWTAWDIERLVL
                     NQFDDIEQVKCISGVTESNAQRYEYLDLHDDGGSFEVSYVNEMEDFISSKVIVVIVPK
                     KLKYLKEHLTIANFRKLEQVKSFLKDKMSPFVTLKVRNPEFEYIRISCDIKFVDTGNE
                     GLYIARLKREITKFICPWYVDESDALEFNKELQVQALKDYIKSLDFVVFITRFSVIQI
                     IENGFQYHIVDSAASQDLTQILKPYRPWSTFIPDQDHLINIVEEEIEVEPTSLQPPVK
                     FKNEYNILSKARILKLKSIGGQKRRTVKENNLPFN"
     gene            complement(3910..4365)
                     /locus_tag="PLG24_02040"
     CDS             complement(3910..4365)
                     /locus_tag="PLG24_02040"
                     /inference="COORDINATES: protein motif:HMM:NF016829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HMZ23692.1"
                     /translation="MDIDQINIKKIDGVRSFLGTGWAFPPDFNINTGETLVVSDFQDI
                     SESLRILFSVTPGERVTHLKYGCDLYAKIFEPIDSTFEFTVKEMITTAINDYEPRVEV
                     LDILTDIKDYADGLVKIEIIYRVRATNTRHNIVFPFYKNEGTSLPEEIS"
     gene            complement(4377..6191)
                     /gene="vgrG"
                     /locus_tag="PLG24_02045"
     CDS             complement(4377..6191)
                     /gene="vgrG"
                     /locus_tag="PLG24_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HMZ23693.1"
                     /translation="MSGSDITGRSPLIRQVDLLSIEVLVDGAPLRDNYIVQSVNIHRK
                     INKISTAEFTFLDGDPTKVNFELEDEEVVVPGKSIVIKAGYHQDNNKIFEGVILKVGI
                     SIKPGGHSEITCFCTHKAQYLTIGRQNKYFIDKKDDEIISEIISDYGLPKSVESTKIK
                     HSQIIQYGCCDWDFILSRLEQNGLILLTDDDKVKVKKPDFNSAPVHLLTHGVEIIKSE
                     LEIDGSFQLKKVKTISWNPDNQELNESVNRDVSINLQGNSGQIEFSVLADKFNKKDFL
                     LQSTGHLPEKDLEEWATAQFQRAKMSRTLGSITCNGTEKFLPDKIVELKGLGKWFDGK
                     AYIREVNHVLQSGSWITELIIGWQKDWYLESRTDIINPATSGQLPAMEGIFIGKVKQL
                     EGDPEGGYRIKLDIPVITGSSDGVWARLAQLHASDSHGAYYVPEIDDEVVVGFLNADS
                     RYPVILGSLYSSKLKSPYEHEDINKIKGFVSRSELKLTYHEEDKSITLETPGGRKFTM
                     DDTAQHIKMEDGFNNSITMDASGITIKTTGRVEIDATMGVQMTGSAMVKMDSSGPISV
                     SGTLVNINGNVSAMVSAPTATLSGQMIATVTGAIVIIG"
     gene            complement(6201..6863)
                     /locus_tag="PLG24_02050"
     CDS             complement(6201..6863)
                     /locus_tag="PLG24_02050"
                     /inference="COORDINATES: protein motif:HMM:NF039279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23694.1"
                     /translation="MPGSSEKLKIYTYTDPNVPASGGSVGYEVELVPENISITYGLKF
                     KVDNPNTNKRVTKFEGYEDSTLKFDFILDATGVTGSQMVIKDEIEKLGKAIYNYVPSI
                     HKPHYLRIHYGSVNFICHLKKCTITYLLFKTDGTALRAKVSLEFDEFVLPANAESRVM
                     SSPDLSHIRFFTDSDNIFVFCDEIYANTNQAVQIARINNIVNFRNIPPGTQVLFPPFE
                     RL"
     gene            complement(6853..7026)
                     /locus_tag="PLG24_02055"
     CDS             complement(6853..7026)
                     /locus_tag="PLG24_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23695.1"
                     /translation="MPVEIKELKVTIEVRPKSLDVREIEKLVLDLFEKERSKVENNIT
                     LKALKKEGIKNAR"
     gene            complement(7033..7527)
                     /locus_tag="PLG24_02060"
     CDS             complement(7033..7527)
                     /locus_tag="PLG24_02060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HMZ23696.1"
                     /translation="MANGGDWDQYIPLSFQFSVTIDGETLSFSEASGLHSEMVVEELR
                     VGGRNDRVYSLPVRVKNTNLVLKRAFNTNIKPSNKVERNKPLYTDWINKVLYEDGNLN
                     VRLELKNIVVKLLDPKGNELMSWEIEGAYPVKWSISNLNATENNVAVETIEFAYQSVR
                     LKVD"
     gene            complement(7796..8248)
                     /locus_tag="PLG24_02065"
     CDS             complement(7796..8248)
                     /locus_tag="PLG24_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HMZ23697.1"
                     /translation="MADFENQWPVAKYHFRVTIDGEQISFQEASGLQAETTPIEYRHG
                     DSEAFHVIKQAGLIKTSNLVLKKGVFESDSRLLDIFNKIYDKSYYTQDDRVDIIVELL
                     DETASTVMTWNITKAFPIKFTGTDLKSTANEVSIETIEFAYEEIKTEL"
     gene            complement(8278..9915)
                     /locus_tag="PLG24_02070"
     CDS             complement(8278..9915)
                     /locus_tag="PLG24_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HMZ23698.1"
                     /translation="MMQYATPGVYIKERNAFTNSVVAVPTAVPAFIGYTEKAVNGTTS
                     LKNVPTRITSLAEYVTVFGGPPRIQFNIKGKSNLDFDLKVEKQTQYLLFNSIRLFFAN
                     GGGTCYIVSIGDYKNGVNAKDFNDTENGGGLHTLLAEQEPTLICAPDAVLLPKADCMT
                     FYQEVLKHCGYTMKSRFGLFDVYDGDQPRSYDKADIITNFREGVGNNFLNYGASYYPW
                     LNTTIVTGSEISFKNISNIPDLVKLLTAEAEQIYLQAVSAEDEVAEAETGGDDKAKGD
                     DKAKKAPAPSVKPAKVYDERSMRLFEAAKFEIEKLKDTAAESLVVDNTLKTISPLYKS
                     ILAAIKDKVNVLPAAAAMAGIYTMVDNSIGVHKAPANVSLIAVTSPTVSITADNQADL
                     NLPINGKAVNAIRTFIGKGILVWGARTLDGNSQDWRYINVRRTMIMLEQSIKNAVENY
                     VFDPNTAQTWVKIRTSIENFLTLAWRTGALVGDTTADAFEVHVGLGVTMTPQDILEGL
                     LRVLVRVAVSRPAEFIEITFEQKLQETGGFVTEPMGA"
     gene            complement(10501..11598)
                     /locus_tag="PLG24_02075"
     CDS             complement(10501..11598)
                     /locus_tag="PLG24_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23699.1"
                     /translation="MLSPGQYTSKWSKWISLKLRHKEKNIKVIDDFELLPSSETELFL
                     TRINAVSRKHNDTYAIFVNDLRSLSLIPTLTNIPEKVNFEGMLNNNNFILHSDTNHLP
                     DRLFVNYINNFKSFRIDEMTLSLHKSDFLKASEMEILELATPVFSVTLPRMESNVPVA
                     FLDARLQKIANVKHEITALSDENSQLACDFTNSSKGACTIQVNGKDVKKIYISGRKRL
                     HFAAEIFLKPDAKSESLMQPERFVPVNYKITFDIKKTYWRYILISKSEERNVYDIKDI
                     KFELNGEEVPTEAIKKTELVNGVRCLHVTFRDEMALRLNTFNHEIMKMKINSVNKWTP
                     STIRAPIPTYKNVQRTERSSGKSYSLLYVYI"
     gene            complement(11604..12116)
                     /locus_tag="PLG24_02080"
     CDS             complement(11604..12116)
                     /locus_tag="PLG24_02080"
                     /inference="COORDINATES: protein motif:HMM:NF025431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HMZ23700.1"
                     /translation="MIHEVIPIIAGELKDYLETKFGSYDDVVIISNLINQDGSIAIRE
                     DNKIVVTLLSIERDGSNLNFGGGMIRTDMPLHINLYMLFSAYFNDYAESLKFLSGVIG
                     FFQGNPQFIIDDNTIKAELFHIDFRELSNLWTCLGAKIVPHVMFKFRTLNMDEERIRE
                     EIPPITGITI"
     gene            complement(12113..12409)
                     /locus_tag="PLG24_02085"
     CDS             complement(12113..12409)
                     /locus_tag="PLG24_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23701.1"
                     /translation="MFRRLYNRIFLPAIRFILPGITPRKLILAIILSVIFSYFFYLFV
                     TTRVAGKEWKIWLWGLFSVFFGVLFTRAVANQIKNYLSRRKEIIELMATNKSKK"
     gene            complement(12416..>13009)
                     /locus_tag="PLG24_02090"
     CDS             complement(12416..>13009)
                     /locus_tag="PLG24_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23702.1"
                     /translation="LNRILSYNQIDKLSASNIAARSGNVGESEFTSLNIIDKKDETYS
                     RVIYEDHQVRLRKYKLIDSLGARGICLHGIGYTGNMRDQQRSALWLAVAEVYGWIPDK
                     LYWYIIGTILGFMSLGFPYSVYKFWQVRNVLAKFRRHLIPVGGIWFLLTFGFFFCFNF
                     IPRNTLGVFLGLGFVLFFVLLILFRIYILRIIRLFKK"
BASE COUNT         3580 a         2657 c         2471 g         4301 t
ORIGIN      
        1 gattaaatgg tagattattc tctttaactg ttcttctctt ctgaccgccg atactcttta
       61 gtttcaggat cctggcctta ctgagaatat tatattcatt tttaaatttt acggggggtt
      121 gaagtgaggt gggttcaact tctatttctt cttccacgat gttgatcaga tggtcctgat
      181 cgggaataaa ggtggaccat ggcctgtatg gttttagtat ctgtgtaaga tcctgagatg
      241 ccgcactatc tacgatatgg tactgaaaac cgttttcaat gatttgaata accgagaatc
      301 tggtgataaa gacaacaaaa tcgagggatt tgatataatc tttcagcgcc tgtacctgca
      361 attccttatt gaattccaaa gcatcggatt cgtcaacata ccatgggcag ataaatttag
      421 ttatttcgcg cttaagtctg gctatatata aaccttcatt gccggtgtcg acaaacttga
      481 tatcgcatga aattcttata tactcaaatt caggattgcg cactttcaaa gtcacaaagg
      541 gagacatttt gtccttaagg aaactcttga cctgttccag tttcctgaaa ttggctatgg
      601 taagatgttc cttaagatat ttcagctttt taggaacaat cacgacaatg accttgcttg
      661 agataaaatc ttccatttca ttgacataac tcacctcaaa tgaccctcca tcatcatgca
      721 aatccaggta ttcataacgt tgtgcattgg attctgttac accggatata cattttacct
      781 gttcaatgtc atcaaactgg ttcaaaacca gtctttcaat atcccaggct gtccagcctc
      841 tgtttttatg ccttaatctt tcacttattc tggtatagaa ttcgatattg ctctcctccg
      901 cttttccgcc gaagctttca tacggttgaa tcatactggt tacaccgtga attttattgt
      961 ccagagcgat gatggattcc ggagggaagg gagtgtttag ccctgaagga tcagactggt
     1021 ccacccacct ggtaagagtt gcgtttggat aaatggccag agtatggtta agtaattgga
     1081 tgtcgcccgt agcactgact cttagccaga atttgtttgg ctccattatc tgagacatag
     1141 tagagatgtc tgacggaatc ttgatttgaa ttactcctga tttggtaaat gagtccgttt
     1201 catccctgag gatattctcc ccctgaagta atttccagtt gttattgtat gtcagatatt
     1261 gccagcggat tataggcagg atatactgat cccgatcctg gtaatagaca ggcgtcatct
     1321 gaaacaacaa agatataatc tctgcctttt tacaattctt caaccccagc aacaggtatc
     1381 cgtcttcctc gtattgcgga agtagataag gcaaccgatc cctgtatttg atatttggat
     1441 agattttttc gtagccgaaa ggatggatat ggttgatttc ctggaagttg acagcatcct
     1501 gggttagttc gattacatca gaagccttgt aattgatatg gatattactc accatcggga
     1561 tgagaggcat ttcggggaat cccaattccg gcgaatcttc attttcatcc tctacaaatg
     1621 ccttgttgat aagtctggca tttttaagca tctgttttgc aagtatctcc tgatattttt
     1681 gctgcccgaa accatcttca ggttcatcta aaacaaattt gaggaaacct acattggcat
     1741 tggtagagaa ttgatctgcg atttccatgg attcagacga attaaagccc aacgatttca
     1801 gatctccgct gtctaatgta tatttggtga ataaaccttc atcgctaagt ttgtcacttc
     1861 cgatgatttc ttttgtcata aacaatggtc tggaattatc ttccatcacg aattggccat
     1921 cattcagatt gcctatctgg actttaaatt catcataccg cctttcgtat acttcatatt
     1981 tttgattaaa ctgtccggca gtttcaggca agtcaaacca atcaatattt aattcaaatt
     2041 cgtccagttt tttactgaaa atttccctgt tgccaagaag gaaataggag tttttgactg
     2101 gtgtaggtcc gaacggatag aatggctgag aattgtcgat aaggccatat tcgttatata
     2161 ccgataagtt gcgtacattt tccacccgag catcaatctc tatggtctcg ataacaagtt
     2221 tttggaggaa ggaatataga tagggttctc tgtcattgtt gagcaatagc atcaaaatcg
     2281 gttgcgagcc tgatatgtat tttccgctgt gcaaatgttc attgaatgga atgatgggag
     2341 gggccatggg agacaagttg aatgagatac aaatatgcct cgggtcccat tcttcttcac
     2401 cgtcctgaaa tatgagaatt gtctgtggat ccaccttatg ccatcccttt gcgctcgtaa
     2461 gaagaatagt aatatttctt ttcttactct ttgtgctgaa tattttataa aatacttcaa
     2521 gcatcgtact tttcttatgc tctttggaag caatatcttt gaaaagtctg acaatttcgg
     2581 ttactgattc ttccgtaaaa ttgagtctta ttttgatgct gcgtttgcct tctccaagca
     2641 tcaaaacgga tgatgcaacg gaaaagccaa gctcagattc aatcatattt ctgagactcg
     2701 gttccttttc tatttgccct tcaccaaatg ttggccatgc cggtagttta tccgaattga
     2761 acggagatcc ctttccatcc tgagaattgg cgtaaggtgc cataaaagac tctgtaatta
     2821 tggaatactt atttgtcagg gtttctcttc ttccccgatc aaaatatagg gtctttatat
     2881 cagaaagtgt ataccgcccg acatttacag gatatttggt gctaaatcta tgcgggaggc
     2941 catccttatc cagggatgcc agcaagatgg tgttttccgg gatggttttg ttttcaacat
     3001 agggtgaaat tgcaaaactt acataggcgt aaggggcgat atgttttctc tttttctgct
     3061 tcagaaaatc gatatagtaa aagtgaaggt aattctcagg aactttattg attgaagttt
     3121 gaagcttttt gtattgttca aggaagccca gcagcagacc tatatgtggt tgatgatcat
     3181 ctttttgtga caatgatctc acaaaataag gaatgagcaa atcccttatt gccaaggatg
     3241 tttcgaggag attattgaag aggatcctca gtctgataaa aacggattct agtttgttcg
     3301 gaatatcttc ctgcgaaaca tcttccgggc tactttcaat tatttggtag cctagattga
     3361 atgctgattt ttgtttttca gcatggggga ttgcaccaag aatgatagcg tgcaattcat
     3421 agatataatc aagtacggat tgttcgtaat acgcttctat gtcgaaataa acgaatttct
     3481 caatttcgcc atcaggatat tgaattttca ggagtctatc ccttaggttg ttaaattcag
     3541 agagaagact attgacgacc gaaatcatag cctctaaagc ttcgatttgg atgttgccat
     3601 ctgttgcccg tgaaaattcc tttaccttca ggttgaagtc aaactgtgcc tgagcgagat
     3661 cttttgttat gctaagagct aaaatggatg ttagatcgta attaaaaaaa gcttgccaat
     3721 ccttactatc atcgctgttt gattctttga aatgcaacag tgatgcgtat tcgtacagga
     3781 tactgagcag attttcaatc ggtcggtcat caatagcgat ttccagcgga ttggtccggt
     3841 tttcaagcct ggtagattgt gtcgtaccta tatatttcgg atctcggatc atgtggattg
     3901 atacattgac taactgattt cttcgggcag actagtacct tcatttttat aaaatgggaa
     3961 cacaatatta tgtcgggtat tggttgctct tactctgtaa ataatttcaa tcttaaccaa
     4021 gccatcagcg taatccttga tgtcggtcag aatgtccaat acttcgacac gtggctcgta
     4081 gtcgttgata gcagtggtta tcatttcttt gactgtaaat tcgaatgttg agtctatggg
     4141 ttcaaaaatc ttagcataca ggtcacatcc atacttcaga tgggtgactc tttcaccggg
     4201 tgtaacggaa aacaaaatgc gcaggctttc ggatatatcc tggaagtcgc ttaccaccaa
     4261 tgtttcacct gtattaatat tgaaatccgg cgggaatgcc catccggtcc ccagaaacga
     4321 ccttactccg tcaatttttt ttatatttat ttgatcaata tccatttaat actatttcaa
     4381 ccgattatca ctattgcccc cgttacggta gctatcattt gtccggatag tgttgcagtt
     4441 ggtgctgaaa ccatagctga tacattacca ttaatattaa ctaatgttcc ggagacggag
     4501 attggtcctg aggagtccat ttttaccatg gcactacccg tcatctgtac acccattgtt
     4561 gcatcgatct ccactcttcc tgtagttttg atggtaattc cggaagcgtc cattgtgatg
     4621 gaattgttga atccatcttc catcttgatg tgttgtgctg tgtcatccat tgtaaatttc
     4681 ctgccacccg gagtttccaa agttattgat ttgtcctctt cgtggtaggt aagtttcagc
     4741 tctgatcttg acacgaaacc ttttatttta ttgatatctt catgttcata gggcgattta
     4801 agtttggagc tgtacaagga accaagaatc accggatatc tggagtctgc attgaggaag
     4861 ccgactacga cctcatcatc aatctccggt acataatagg ctccatggct gtcagaagcg
     4921 tgcagttgtg ccaatctggc ccatacgccg tcagaggagc ctgtaatcac gggtatatcg
     4981 agcttaattc tgtatcctcc ttcaggatcc ccttccaatt gttttacctt gccaatgaat
     5041 attccttcca tagccggtag ttggccggag gtggcgggat tgatgatatc ggttctggac
     5101 tctaggtacc aatctttttg ccaaccaata atcaattctg tgatccagct cccgctttga
     5161 agaacatgat tcacttcccg gatatatgcc tttccatcaa accatttgcc taggcccttc
     5221 aattccacaa ttttatcagg caggaatttt tcagtcccat tgcaggtgat ggaacctaat
     5281 gttctggaca tcttcgccct ctgaaactgg gcggttgccc attcctccaa atctttttca
     5341 ggaagatgac ctgtactttg gagtaagaaa tcctttttat tgaacttgtc tgccaggacc
     5401 gaaaattcaa tttgcccgga attgccctgg aggttgatac taacatcccg attaacggat
     5461 tcattcagct cctgattatc ggggttccag gaaattgttt ttactttttt aagctgaaat
     5521 gagccatcaa tttccaattc agatttaata atttctactc catgagtcaa taaatgtacg
     5581 ggtgcagagt taaaatcagg tttttttacc ttaactttgt cgtcatcagt aagaagtatc
     5641 aatccgtttt gctcaagcct tgataagata aaatcccaat cgcaacatcc atattgtatg
     5701 atctgactat gctttatctt ggtgctttca acagactttg gcaacccata atcactgatg
     5761 atttctgata taatctcatc atcttttttg tctatgaaat atttattttg acggccgatt
     5821 gtcaaatact gagctttatg ggtacagaag caggttatct cagaatgacc acccggtttt
     5881 attgatatac ctacttttag aattacccct tcaaaaatct tgttgttatc ctgatgatag
     5941 ccggccttga ttactatact ttttccgggg acgacaactt cttcgtcttc cagttcgaaa
     6001 ttgactttgg tggggtctcc atccaggaaa gtaaactcag cagttgaaat tttatttatt
     6061 ttccgatgga tgtttaccga ttgcacgata taattatccc gtaaaggtgc tccatcgacc
     6121 agtacctcta tggagagcag gtcaacctgt cttatcaatg gcgaacgtcc tgttatatca
     6181 cttccactca tagcttaagg ttataatctt tcaaagggtg gaaataggac ttgtgttccc
     6241 ggagggatat ttctgaaatt aacgatattg ttgatccttg caatttgcac tgcctggttt
     6301 gtattggcat atatttcatc acaaaaaaca aagatattat cgctgtcggt aaagaatctt
     6361 atatgagaca aatcaggaga agacataacc cgactttccg cattggcagg cagcacgaac
     6421 tcatcaaact ccaggctgac ttttgctcta agggcggtgc catccgtttt gaataaaaga
     6481 taagttatcg tacacttttt aagatgacaa ataaaattga ctgatccgta atgtattctt
     6541 aagtaatgtg gtttgtggat gctcggaaca taattgtata ttgctttgcc tagtttttca
     6601 atttcatctt ttatgaccat ctgacttcct gtaacgcctg tagcatccag gataaagtca
     6661 aatttgagag tactatcctc atatccttca aattttgtaa ctctcttatt tgtatttgga
     6721 ttatctacct tgaattttag tccataggtg attgagatat tttccggcac caactcaact
     6781 tcatacccca cggagccacc actggcgggg acattcgggt cagtataagt atagatcttc
     6841 aatttttcac tactacctgg cattttttat tccttctttt tttaaggctt tcagggtgat
     6901 gttgttttca actttcgacc tttctttctc aaacaaatcc agcacgagct tttcaatctc
     6961 cctgacatcc aaacttttgg gccttacttc aatagtaact ttcaactctt taatttcaac
     7021 aggcatctca gattaatcta ctttcaggcg gacggattga taagcaaatt caatggtttc
     7081 gacagctacg ttattttctg tcgcgtttaa gttggagatt gaccatttca cgggataagc
     7141 tccttctatt tcccaactca tcaattcgtt tccctttgga tccaataatt ttactacaat
     7201 attctttaat tcaagccgca cgtttagatt accatcttca tataatactt tatttatcca
     7261 atcggtataa agtggtttgt tcctttcaac tttgttactg ggtttgatat ttgtattaaa
     7321 agcccgcttt aacacaagat tggtattctt gaccctcacc gggaggctgt acactctgtc
     7381 attcctgccc ccaaccctta actcttcaac gaccatttcg gaatgaagtc cacttgcctc
     7441 tgaaaaactc agggtttcac catcgattgt caccgaaaac tgaaatgaca acggaatata
     7501 ctgatcccaa tctcctccat ttgccatgat tacttattta ctctttctgt aaagatttgg
     7561 ttacagttta tttaacacat actgattgaa gactatctgg atgttggggc agtttcaata
     7621 tttatttcct attcctgact tgataaaagt aaataatcgg gacatgttga tccgcgattg
     7681 atttatatca aagtcataag aaaatggaag gttcctatcc actcccaatt ataacagccc
     7741 caatccaaat acaaatcaac cttgaaagaa acttccaagg ttgatttagt aaatattaca
     7801 actcagtctt gatttcctcg taagcaaatt cgatggtttc aattgaaacc tcatttgctg
     7861 tggacttcaa gtctgttccg gtaaatttga ttgggaatgc tttagtaata ttccaagtca
     7921 taacagtgga agcagtttcg tcgagcaatt ccacaatgat atctaccctg tcatcctgtg
     7981 tatagtaact cttgtcgtaa attttgttga atatatccaa caatcgacta tctgattcaa
     8041 aaacgccttt cttaaggacc agattacttg ttttaatcaa tccggcttgc ttaatgacgt
     8101 gaaatgcctc actatcccca tgcctatatt caattggggt tgtttcagcc tgcaggcctg
     8161 atgcttcctg aaagcttatt tgttcgccat ctatggtaac ccgaaaatga tacttcgcta
     8221 ccggccattg gttttcaaaa tctgccattt tatttatttt ttaggattat caaaaaatta
     8281 tgcacccatt ggttctgtaa caaaacctcc ggtctcctgc agcttctgtt cgaatgtaat
     8341 ttctatgaat tctgccggtc tgctaactgc tactcttacc aataccctga gaagtccttc
     8401 gaggatatct tgaggagtca tggtgacccc taagccaaca tgtacttcaa aagcgtctgc
     8461 cgtagtgtct cctaccaatg cacctgttct ccatgccaag gtcaggaagt tctctatgga
     8521 tgttctgatt ttaacccagg tctgggctgt gtttggatcg aaaacataat tttctactgc
     8581 attcttaatg gattgctcga gcatgatcat agtccttctg acgttgatat atcgccagtc
     8641 ttggctgtta ccgtcaagtg ttctcgctcc ccacaccaag attccttttc ctatgaatgt
     8701 ccttattgca ttgactgcct taccattgat ggggaggtta aggtctgcct gattgtccgc
     8761 tgttatagac actgttggtg atgtaactgc tatcagggaa acattggcag gggccttatg
     8821 tacaccaatt gaattatcta ccatggtata aattcctgcc atggctgctg ctgccggcag
     8881 cacattgacc ttgtccttga tggcagccag aattgatttg tacaacggac tgattgtttt
     8941 cagcgtatta tccaccacca ggctttctgc tgcggtatcc ttaagttttt caatttcgaa
     9001 tttggcagct tcaaataatc tcatggagcg ctcatcataa accttggccg gtttgacgga
     9061 aggtgcaggt gcttttttag ccttatcatc tcctttagcc ttatcatcac cacctgtttc
     9121 agcttctgca acctcatctt cagcactcac cgcctgcaga tatatttgct cagcctctgc
     9181 agttaacagt ttaacaagat cggggatatt gcttatattt ttgaatgaaa tctcagaacc
     9241 ggtgacgatt gttgtattca accagggata ataggatgct ccataattga ggaaattgtt
     9301 gcctactcct tcgcggaagt tggttatgat atctgcctta tcataggaac gaggctggtc
     9361 tccatcatac acatcgaata acccaaatct tgacttcatg gtatatccgc aatgcttcag
     9421 tacttcttga tagaaagtca tacaatcggc ttttggcaga agtacagcat ccggtgcaca
     9481 aatcagtgtg ggttcttgtt ctgcaagtaa cgtatggagg ccgccgccgt tttccgtgtc
     9541 attgaaatct tttgcattga ctccattctt gtaatctccg atggaaacta tataacaagt
     9601 acctccacca ttggcaaaaa agagcctgat gctattgaat aataaatatt gagtctgttt
     9661 ttcaaccttg agatcaaaat ccaaattgct tttacctttg atattaaatt gaatcctcgg
     9721 tggtccgcca aagacggtta catactctgc cagagaagta atccgcgtag gcacgttttt
     9781 gagcgatgta gttccattta cggctttttc agtatagcca ataaatgctg gtacggctgt
     9841 tgggacagcc actaccgaat tggtgaaggc gtttctttcc ttaatgtaaa cacccggcgt
     9901 tgcatattgc atcataatca aaagttttta aatttattat ttacctattt gtataatgag
     9961 cttaattaaa aattattgaa ttgtgggtta aaatattttt aaacaatccc gtttgtcatt
    10021 gtttaaataa tatgatagtt gtggtgaatt tatcttaaga cctttagttt aaatcccgca
    10081 tgaaataact aatgtaacta aatttgcaaa tatttgttct gtactatttg gtataaacaa
    10141 atattaattc agtctgtaaa tttagttatt aaactaaata aaaacaattt tttttcctga
    10201 tattttaaat atttaagaaa aaagattcgt ggggctttga agattaaggg acaaaaatta
    10261 aaagctgaaa atcaatacga tagtaaaaaa atattccgtt ttgccatata agcaaaaaac
    10321 taatttataa taaattgtat atcaacattg ttgttgtttg gttgttattt tgctttgaat
    10381 tcaaagggaa gtggacggat cactaaatat cttgcagttg acgctcagaa atatccacct
    10441 ttacctggag gtgttatggc aaccatttcc tatctcaaac gcgatttttg agttgtgacg
    10501 tcaaatgtac acatacaaca gtgaataaga tttgcccgat gatctttccg tacgctgtac
    10561 atttttatag gtaggtattg gggcacggat agtagagggg gtccatttat tgacgctgtt
    10621 aatcttcatc ttcatgattt catgattgaa tgtattcagc cttaatgcca tttcgtccct
    10681 gaaagtcaca tgtagacacc gcacaccatt gactaattct gtttttttaa tagcttctgt
    10741 tggaacttcc tcaccattca attcaaattt aatatcctta atgtcgtaca cattcctttc
    10801 ttccgatttg gagattaaga tatagcgcca gtatgtcttt ttgatatcaa aggtaatctt
    10861 gtaattgaca ggtacaaatc tttcaggttg catcaatgat tctgacttgg cgtccggctt
    10921 aagaaatatt tctgcggcaa agtgtaatcg ctttctcccc gaaatataga tcttctttac
    10981 atccttccca ttgacctgaa tggtacatgc gcccttcgag ctattggtaa aatcacatgc
    11041 caattgagaa ttttcatcag acaacgccgt tatctcgtgt ttgacattag cgattttctg
    11101 gagtcgtgca tcaagaaatg caaccggcac attactttcc attcggggca aggtcacact
    11161 gaacaccggg gtggccagtt caagtatctc catttcagaa gctttaagaa aatcactttt
    11221 atgaagtgac agggtcattt catctatccg gaatgacttg aaattattga tataattgac
    11281 aaaaagtcgg tccggaagat ggttggtatc actgtgcaga ataaaattat tgttgttgag
    11341 catcccttcg aaattgactt tttcggggat attagtaagc gtagggatta aggacaatga
    11401 ccgtaaatca ttgacgaaaa tggcataggt atcattgtgc tttctggata ctgcattgat
    11461 tctggttaag aacagctctg tttctgaact tggaagtaat tcgaagtcat caatgacttt
    11521 gatgtttttc tccttgtgcc taagtttcaa ggaaatccat ttggaccatt tgctggtata
    11581 ttgacccggt gacaacatga caattagatt gttattcctg taattggtgg tatttcttct
    11641 cttatccttt cttcatccat attgagtgtc ctgaatttaa acataacatg cggaactatt
    11701 tttgctccga ggcatgtcca caggttgctc aactctctga aatctatatg aaacagttcg
    11761 gctttgatgg tattgtcatc gataataaat tgcggattcc cctgaaaaaa accgattact
    11821 ccggacagga atttcagaga ttccgcatag tcgttgaaat acgcactaaa caacatatag
    11881 aggttgatat gtaatggcat atccgtgcgt atcatccctc ccccaaaatt caggttagaa
    11941 ccatctcgtt ctatactcaa cagagtaact acaattttat tatcttccct gattgctatg
    12001 ctaccatcct gattgatcaa attggaaatg attactacat catcgtagga accgaacttt
    12061 gtctcaagat aatctttcag ttctcctgca attattggta ttacttcgtg aatcattttt
    12121 tgcttttatt ggttgccatt agttctatta tttcttttct tctggataga tagttcttga
    12181 tctgatttgc taccgccctg gtaaagagga cgccgaaaaa aaccgaaaaa agaccccaga
    12241 gccatatttt ccattctttg ccggctactc gtgttgtgac aaagagatag aaaaaatagg
    12301 aaaaaatcac actgagaatg attgcaagaa tgagtttccg aggtgttatc ccgggaagta
    12361 taaaacggat tgccggtaaa aaaattctat tgtacagcct tcggaacatc tctcattatt
    12421 ttttaaacaa acgaatgatt ctaagtatat atatcctgaa cagaatcagt aatacaaaaa
    12481 acaggacgaa gcctaatccc aaaaagacac ctaaggtatt cctgggtata aaattaaaac
    12541 aaaaaaagaa gccgaaagta agcaggaacc aaataccgcc aaccggaatc aggtgccttc
    12601 taaacttagc caatacattc cgtacttgcc agaacttata tacagaataa ggaaatccaa
    12661 gtgacataaa tcccaggata gtcccaatta tataccagta taatttgtcg ggtatccagc
    12721 catagacttc ggctactgcc agccacaaag cacttctttg ctgatcccgc atattgcctg
    12781 tatatcctat accatgcagg catatacccc gggcgcccag ggagtcgatg agtttgtatt
    12841 tcctaagcct gacctgatgg tcctcgtaaa ttactctgga ataggtttcg tcttttttgt
    12901 cgatgatatt caatgatgta aattcagact ctccaacatt gcctgatctt gctgcgatat
    12961 tgctggctga aagtttatca atctgattat aggataaaat cctgttcag
//
LOCUS       DAOQQN010000031        12855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1671_length_12855_cov_5.849922, whole genome shotgun sequence.
ACCESSION   DAOQQN010000031 DAOQQN010000000
VERSION     DAOQQN010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12855
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1671_length_12855_cov_5.849922"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PLG24_02095"
     CDS             complement(<1..840)
                     /locus_tag="PLG24_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HMZ23703.1"
                     /translation="MSKRHIYLDNNATTPTDPRVIEAMLPYFYEKPGNPASRNHPFGW
                     IAEEAVDYAREQIADLIHVDPKEIIFTSGATESDNLALKGVFEMYRSKGNHIVTAKTE
                     HKAVLDSCRKIEKMGGEVTYLDVERDGRIDLDKLEAAIKPETILVSIMWANNETGVIQ
                     DMKAIGDVCKKHGVLLMSDATQAVGKIDVDPKAAGVHLMAFSSHKMYGPKGIGALYVC
                     RKSPRVKVTAQIDGGGHERGMRSGTLNVPGIVGFGKAAKIAREEMAADAARLSKLRDK
                     LQNA"
     gene            958..1899
                     /locus_tag="PLG24_02100"
     CDS             958..1899
                     /locus_tag="PLG24_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HMZ23704.1"
                     /translation="MEDRTSIFPQFKHADWRKGFSREVGEAHLNEMNFTIDGLSFEPM
                     GDDEDAESFAGKLPKKEENIAFGEIFDFTGGNQPIRQLMESLQHDYTAPLLLVDKKTN
                     AGIFDDVRFDILNASIHVTDGWMDPWKVYFMILHGGYPKGLRIINRKDDSGKDALKHF
                     HFDAKNEVIPFIRQISREIEMTNHQNIQISMTFTGRTLHDIIKARAVKTSWFNILYHA
                     GLPLTPFHFEVILPVEGQYPDIVIGHTNNLIASTMAQADTVVFACRQDDNERRILRNS
                     AHISVIEAGLDKETDPVAGSYFVEKVAAQLAEKALAG"
     gene            complement(2137..4734)
                     /locus_tag="PLG24_02105"
     CDS             complement(2137..4734)
                     /locus_tag="PLG24_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HMZ23705.1"
                     /translation="MCSFEIKHFQSITVKSFLFTLIVAFSSVQSGVSQNPFPVWPDSI
                     FSAYYHQRAGLFNVMSGTKGGVYFVGNSITDGAEWSEIFGDANIRNRGISADISAGVL
                     NRIQTLIERRPDKVFLMIGINDLARGIAPDSILHNIGTFVRLLREYSPTTQIFIQSLL
                     PVNPDLKMFAQHTSKAAEIKYVNGILEREAGQGYAYIDLHTHFLDEDGKLSRRWTNDG
                     LHLNGDGYMLWKHIVFPLVYDLQAHPAIIPQPLDISWLEGSFPLYKLKNITIPRSGLD
                     NEAVILKDFLGSLGLYPRIIPTGSPEGPVIVLNLDPAYDGPSQEAYSIMVSPQRVEIR
                     AKGAHGILHGIQTLKQLCRDGVMVPCCIIKDAPAFQWRGFMIDVGRNYVSPDILKQII
                     DKMSFYKFNIFHFHPTEDIAWRLESRLYPQLTAPEYMLRDKGLYYTVAEVKSLIEFCR
                     ARHITFVPEIDMPGHSAAFRRAMGVDMQSDSGTVLVKKIIGELIDDYPLQYIHVGGDE
                     VKIENKDFLPDIVAMIQGRGLRTIAWSPGGETNSHTMRQLWMDDDGHNNKAGELMYID
                     SRHLYLNHMDPLEAVTTIYYRKIGERTRGDTAFPGATLCLWNDRAVADGSDILSMNPV
                     YPGMMAFAERVWNGGGRSGWIAGIPLDDVPVFSKFESGLLDHKALYFNNLPFPYVRQS
                     NLRWTLIGPYANGGDFSIRFEPEISGKPGTGRMIPAVGGTIVFRHWWAPQISGALQNP
                     EENTTWYATTTIQSDVDTVRGFWIGFNDLSRSPATDSPPLGGWNHRQGKLWVNGHEVP
                     PPIWRHPGQQGNSEIPLVDEGYSYRPPTMIHLKKGANTVLVKSPIGKFRGKDWQNPEK
                     WMFTFVPVQ"
     gene            complement(5278..6453)
                     /locus_tag="PLG24_02110"
     CDS             complement(5278..6453)
                     /locus_tag="PLG24_02110"
                     /inference="COORDINATES: protein motif:HMM:NF025059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HMZ23706.1"
                     /translation="MEQTDKIDEFLKVLAEATQASSFNSLTLSKPREKHGKIKSLYFR
                     PVMIRNEVKTQMVVRFSDREDTKNFSQTEFLAFASQSTRQHFFYAHLVTSSQEGHLLI
                     SRRGFVTLKWKQLTREMIALRVHDKEKQYIIPPEEPFLQHLGISSSTGTIIKDQYKKY
                     RQISRYIELFAPLTEGLDKTVPLEVTDMGCGKGYLTFAMHRFLKGAGFKQVHTSGIDI
                     KSDVIRNNNIIAREVGYDGLEFLHGRIEENRDINPDILIALHACDTATDDAIFYGVER
                     KAKVIVVAPCCHKQVRKSMARNELTSGLFRHGIIQERWASDLTDMIRANILRYLGYQV
                     KIMEFVSIEHTPKNIMITAQYYGKINETARQEIYTWMEMFGIGEQYLLNKLGLAPDN"
     gene            6841..7548
                     /locus_tag="PLG24_02115"
     CDS             6841..7548
                     /locus_tag="PLG24_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HMZ23707.1"
                     /translation="MNTRESEIRHNLAVIHERIRKACRDCNQAEEDVNLLLATKTVSP
                     DDIRIAVEAGATLIGENKVQEFSSKADALADLCITRHFIGHLQRNKVKEVLRHVQCIQ
                     TIDNLALAEEVNKRAGAMGRSVEVMIQVNTSFEASKFGIAPENLPELAEQVRNMQNLT
                     LIGLMTIGLNAEVIEDARPSYALLKELGEKLNDKYPEKPIREYSMGMSGDLEIAIEEG
                     ATIIRVGSAVFGTRIYP"
     gene            7803..8972
                     /locus_tag="PLG24_02120"
     CDS             7803..8972
                     /locus_tag="PLG24_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HMZ23708.1"
                     /translation="MQFSLTEEHLAVRDTARDFAQKELLPGVIERDSKMIFPTEQVKM
                     MAELGFLGMMVSPEYGGGGMDTISYVLAMEEISKVDNSCSVLMSVNNSLVCWGLEAFG
                     TEEQKQKYLTRLATGEIIGSFCLSEPEAGSDATSQRTTAVDMGDYYLLNGTKNWITNG
                     GSSKIHLVIAQTHPEKGHHGINAFIVETGWDGVVIGAKEDKLGIRSSDTHTIMYNDVK
                     VPKENRIGEDGFGFKFAMKVLSGGRIGIASQALGIASGAFELATAYSKERKSFGKEIY
                     NHQVIAFKLADMATEIEAARLLVHRAAWLKDQKKDYNTASAMAKLFAAETAMKTTVEA
                     VQIHGGYGFVKEYHVERLMRDAKITQIYEGTSEIQKLVIARSVINGELYLPGYVV"
     gene            8995..10107
                     /locus_tag="PLG24_02125"
     CDS             8995..10107
                     /locus_tag="PLG24_02125"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HMZ23709.1"
                     /translation="MAIFEKGSMYAGTYFGIPVKIHWTFGYIIFYIIYSGYQDNLGLQ
                     GMLWFGLFILSLFLCVILHEYGHALTARRYGVHTADIILTPLGGIARLTKMPDKPVQE
                     LIVALAGPLVNVIIAGLLFVGLYFGFEYNPFIIDVDNAYNFTGNPGGFFGMLMLTNIA
                     LVVFNLLPVFPMDGGRVLRSLIAMKASKVRATLIAARTGQLFSLFFVGYGIYSGNLML
                     ALIGLFIFQSAYMEYRYTKADNLMQSSIVENLGYAEYDTFQSDDLIINCKELMKYSDQ
                     PFFPILSNEVVIGSVSRDDIIQSDLLYRPVSELSMMPVPQLPADTNWMTCYQIFSKDQ
                     ADVILLNKDGAKPTLLDKWIFYQQLENKKLIKKGKL"
     gene            complement(10129..10914)
                     /locus_tag="PLG24_02130"
     CDS             complement(10129..10914)
                     /locus_tag="PLG24_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HMZ23710.1"
                     /translation="MIFFLITFSGQAQDAIPPYDSLVIQSELLGESRAINIWTPPGYA
                     ISKASYPVLYMPDGAITEDFPHIANTLSELILAGKIPPTILVGIPNTQRRRDLTGPTT
                     VRRDKKIAPVVGGSTAFRMFIRDELFPEIASRYRTNGQRTIIGESLAGLFIIETFLMA
                     PDMFDNYIAFDPSLWWNKHQLVNGAAESLKHFPKAEKRLWFAGSSVQDIYPHTRQLAE
                     ILRNANLGNLKVNYSDEPGEKHNTIFRATKVKALEWTLGNDSR"
     gene            complement(11291..12028)
                     /locus_tag="PLG24_02135"
     CDS             complement(11291..12028)
                     /locus_tag="PLG24_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23711.1"
                     /translation="MTEQKQFVATEEAKGQAKQLRLFAMLAWIVAIAGEVFAILKLLR
                     NDMLTWLIVTIVGILALSIVGSILWKRANRLDPASEKDKTRFFIQNQLGAIMGVLSFL
                     PLVILIFTNKDLDKKTKGIAGAVGVVALLIAGITGVDFNPPSIEKYTEQMNAQTATLK
                     DLNNGVDMVYWTNQGNKYHIFEDCQHIKNREIHNGTVEDAFKARKIGDNELCKTCMAR
                     AEKMKTGTPAGNEETVPAPVPEEATQN"
     gene            12268..>12855
                     /locus_tag="PLG24_02140"
     CDS             12268..>12855
                     /locus_tag="PLG24_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23712.1"
                     /translation="MKRAIHIILFLFGLTSLVPLQAQTKVELNISHLFGHQPFAPENV
                     GINDLNTPFKIERLEYYISDIILVHDGGVETKVPDYWILADISKRGSYELGSFDIKHL
                     EKVIFHIGVDPEHNNADPSVYPADHPLALKEPAMHWGWAAGYIFIALNGNGGDNLDNP
                     FQVHALGNENYYAVELEMNIDAVNGSLTIPIDANYD"
BASE COUNT         3678 a         2935 c         2669 g         3573 t
ORIGIN      
        1 agcattctgt agcttatctc tcaacttgct gagtctcgct gcatcagcag ccatttcttc
       61 cctggcaatt tttgctgcct taccaaatcc aacaataccg ggaacattga gtgtacctga
      121 acgcatacca cgctcgtgcc cgccaccatc tatctgggcg gtaaccttaa ctctcgggct
      181 tttacggcat acgtacaatg caccgattcc ttttggcccg tacattttgt gtgaggagaa
      241 tgccatcaaa tgcacgcctg cggccttagg gtcaacatca atcttgccga ctgcctgagt
      301 cgcgtccgac atcagcaaca caccatgctt cttgcaaaca tcgccgattg ccttcatatc
      361 ctgaattacc ccggtttcgt tgtttgccca cataatgctg actaagattg tctccggttt
      421 gatagcagcc tcaagcttat caagatctat gcgcccatcc ctttccacat caaggtaggt
      481 cacctcaccg cccattttct caatttttct gcaactgtca agtacagcct tatgctctgt
      541 cttggcggta acgatgtggt tccccttgga acgatacatt tcaaaaacac ctttcagggc
      601 aagattgtcc gactcggtgg cccctgaagt aaagattatt tccttcggat ccacatggat
      661 caaatccgca atctgctccc tcgcatagtc aactgcctct tctgcaatcc agccaaaagg
      721 gtgattacgg ctagccggat ttcccggttt ttcgtaaaaa taaggcaaca tagcctctat
      781 aactctcgga tctgtaggcg ttgttgcatt attatcaaga tatatatgtc ttttgctcat
      841 catttatttt taaataatct aaataaaatc cgtgcaaata taactacaaa aaaagtatta
      901 agttcaacaa ttccagtgta tttgtttcta cctttgctcc tattagaaat ttttgtaatg
      961 gaagacagga catctatttt cccccaattt aagcatgccg actggagaaa aggattctcc
     1021 agggaagtgg gagaagcgca tctgaatgaa atgaatttta ccatcgacgg cctctcattc
     1081 gagccaatgg gtgatgatga agatgcagaa agttttgccg gaaagttgcc aaagaaagag
     1141 gaaaatatcg catttggaga aattttcgat tttaccggag gaaatcaacc gatccggcaa
     1201 ttgatggaat cattgcaaca tgactatacc gctccgttgc ttttggtgga taagaagacc
     1261 aatgccggga tctttgatga tgtccggttt gatatcctga atgcttccat acatgtcaca
     1321 gacggctgga tggacccctg gaaggtttat tttatgatat tacacggagg atatccaaaa
     1381 gggctacgta tcatcaatcg aaaggatgat tcaggaaaag atgccttgaa gcattttcat
     1441 tttgatgcaa aaaatgaagt cattccattc atccggcaaa tttcaagaga aatagagatg
     1501 acaaatcatc aaaacataca gatttccatg acattcaccg ggcgcacctt acatgatatt
     1561 atcaaggcaa gagcagtaaa aacatcctgg ttcaatatcc tttatcatgc cggtttgcct
     1621 ttgacaccct ttcattttga ggtgattctg ccggttgagg gtcaatatcc tgatattgtc
     1681 atcggccata ccaataatct tatagcttca acaatggctc aggccgatac cgttgtattc
     1741 gcttgcaggc aagatgacaa cgagcgacga atcttgagaa attctgccca tatttcagtc
     1801 attgaagcag gcctcgacaa ggaaacggat cccgttgccg gtagttattt cgtggaaaag
     1861 gtagccgcgc agttggccga aaaggctctg gccggataat tcaccaacag gccctacttt
     1921 atttttttta ccatattcac aacttttgcg gaatttacac caaaaagaca gtcggtgaaa
     1981 agtgaaattt ttataccaca acaatcaaac aggataatat taacccgctg taacccaaca
     2041 tacataccaa tacaattgta gctcagaaat ttctgctatt tccccatata ttcattggta
     2101 caatatctcc ggtcttcaag acaaactgca gtcgtattat tgtaccggaa caaaggtaaa
     2161 catccatttt tcaggattct gccaatcctt tcctctgaac ttgcctatcg gtgacttgac
     2221 cagcacggta tttgcacctt tcttaagatg aatcattgtc ggtggcctgt aactgtaacc
     2281 ttcatccacg agcggaattt ccgaattgcc ttgctggccg ggatgacgcc aaataggcgg
     2341 tggcacttcg tgaccattga cccaaagctt accttgtcga tgattccatc ctccgagtgg
     2401 cgggctatcc gtagccggag atctcgataa gtcattgaaa ccaatccaaa atccgcgcac
     2461 cgtgtccaca tcgctttgta tggttgttgt cgcataccag gtcgtgtttt cttccggatt
     2521 ctgaagcgct ccgctgatct ggggcgccca ccaatggcgg aaaacaatag tgcccccaac
     2581 agcaggaatc atccgtccgg tacccggctt acctgaaatt tccggttcaa acctgatcga
     2641 gaagtcacca ccattggcat agggaccaat gagggtccag cggagattcg attgcctgac
     2701 atatggaaag ggaagattgt tgaaatacaa tgctttatgg tccaacagtc ctgattcaaa
     2761 cttgctgaat accggtacat catcgagcgg aatgcctgca atccatccgg accgaccccc
     2821 tccattccaa acccgctctg caaaagccat catgcccgga tatactggat tcatgctcag
     2881 aatatcgcta ccatctgcta cagcccgatc gttccacaaa caaagagtag ctccgggaaa
     2941 ggcggtatca ccccttgtcc gctcgccaat ctttctgtaa taaatcgtag taacagcctc
     3001 caacggatcc atgtgattga ggtaaagatg tcttgaatca atatacatca actctccggc
     3061 cttattgttg tgaccgtcat catccatcca caactgccgc atagtatggg aattggtctc
     3121 cccgcccgga ctccaggcaa tggtacgcaa tccccgcccc tggatcatcg ccacgatatc
     3181 cggaagaaaa tccttatttt caatcttcac ctcatctcca ccaacatgaa tatattgcag
     3241 gggatagtca tctatcaact caccaatgat cttctttacc aacaccgtac cggaatcgct
     3301 ctgcatatcc acacccatcg ctctgcgaaa agcggcactg tgccccggca tatctatctc
     3361 cggcacgaag gtaatgtgtc gtgccctgca aaactcgatc agtgacttta cttcggcgac
     3421 agtataatac aggcccttat cacgcagcat atactccggt gcggtaagtt gtggataaag
     3481 cctgctttcc aaccgccaag cgatgtcttc cgtaggatga aaatgaaata tgttaaactt
     3541 ataaaaactc attttgtcga taatctgttt caaaatgtca ggagaaacat agttccggcc
     3601 aacatctatc ataaagcctc gccattggaa agccggtgca tccttaatga tacaacaggg
     3661 aaccatgaca ccatctctgc agagttgctt taaggtctga ataccatgta agatgccatg
     3721 tgcacctttt gcccgtatct caaccctctg tggactgacc atgatgctat atgcttcctg
     3781 agaagggcca tcataagcag gatcgaggtt taacacaatg accggccctt ccgggctgcc
     3841 tgtcggtatt attcgggggt atagtccaag actgcccaaa aaatccttta gaatgacagc
     3901 ttcgttatca agcccggacc ggggaatagt aatatttttc aatttgtaca atggaaaaga
     3961 accttccagc cacgagatat ccaaaggttg aggaataatg gcggggtgag cctggaggtc
     4021 ataaaccaag ggaaacacaa tatgcttcca taacatatag ccatcaccat tcaaatgcag
     4081 gccatcattg gtccatcgcc tcgacaactt accatcctca tccagaaagt gagtgtgcag
     4141 gtctatgtag gcataaccct gtccggcctc cctctccagt atgccattga catatttaat
     4201 ctctgctgcc ttcgatgtgt gttgtgcaaa catcttcagg tcgggattga cgggcagtaa
     4261 actttggata aatatctgtg tcgtcggact atattcccgc aaaaggcgaa caaaggtgcc
     4321 aatattgtgc agaatactat ccggtgcaat tccgcgtgcg aggtcattga ttccaatcat
     4381 tagaaagacc ttatccggtc ttctttcgat caatgtttga atgcgattga gcactccggc
     4441 ggagatgtct gcactgatgc cgcgatttct gatgtttgca tcaccgaata tttcactcca
     4501 ttcggcacca tccgtgatac tgttaccgac aaagtacacg cctcccttag tgccgctcat
     4561 cacattaaaa agtccggcac gctgatgata ataagcggaa aatatggaat ccggccatac
     4621 cggaaaagga ttttgagaaa ctcctgactg aacggacgaa aatgcaacga ttaaggtaaa
     4681 caaaaaactt tttactgtaa tggattgaaa atgttttatt tcgaaactgc acataatacc
     4741 tattgaattc aataataaat gtataaaata attactattc ttaaccagga agaagtcaat
     4801 aaacaactga ccggctatgc agaaacaatt tagattcttg ctaggaatgc accatcagac
     4861 aaaaaatcgg gctaactata acggcatgta agattaaaaa agaaaatctc ttaatcagaa
     4921 atcatgatga tataaaattt caaccttctc ccggatttga tctcctcatt attccggaat
     4981 acacaatcag cttactcgct aaggatccta cctttattca cttggaacat gccgaaaatc
     5041 gatcatcgcg accagttact aagattctga ttacctgtca aatagctcaa tctcgtaaca
     5101 aatatcttat tgtatggtga catggattcc ttatttcttc aaaatatata caaagcaata
     5161 gagtaaaaca taagaccgtc gagatattac tcttctttca gttggggcag attggattag
     5221 aatcatgttt ttttaaaaaa acaggtaaga atgcctttta caacaccatc tttccattca
     5281 attgtcggga gccaggccga gtttattcaa tagatactgc tcacctatac caaacatctc
     5341 catccaggta tatatctctt gtctggctgt ttcattaatc ttgccataat attgggcggt
     5401 aatcatgata tttttcggag tatgttcgat actcacgaat tccataatct tgacctgata
     5461 ccccagatag cgaagtatat ttgccctgat catgtctgtc aggtcgctgg cccatcgttc
     5521 ctggatgata ccatgacgga atagacccga ggtaagttca ttgcgtgcca ttgatttcct
     5581 gacctgctta tgacaacaag gagctacaac aatgactttg gcttttcgtt cgactccata
     5641 aaaaattgcg tcatcggtag ctgtatcgca ggcatgcagg gcgatcagga tgtcaggatt
     5701 aatatccctg ttctcttcga ttcggccgtg caaaaactcc agaccatcgt agcctacttc
     5761 ccgggcgatg atgttgttat ttcggatgac atccgacttg atatcgatac ctgatgtgtg
     5821 tacctgcttg aatcccgccc ctttcaaaaa gcgatgcatt gcgaaggtaa gatacccctt
     5881 accgcaaccc atatcagtaa cttccagagg cacagtctta tccaaacctt ccgtgagagg
     5941 agcaaaaagc tctatgtaac ggcttatctg gcgatacttc ttatactgat ccttgataat
     6001 tgtacctgtt gaggagctga taccaagatg ttgcaaaaag ggttcttccg gcggaatgat
     6061 gtattgtttt tctttatcat gaacccggag tgcaatcatt tccctggtca gttgcttcca
     6121 cttcaaggtg acaaagccac gtctggagat caaaagatgc ccttcttgac ttgaagtgac
     6181 caggtgtgca taaaaaaaat gttgtctggt actctggctt gcaaaggcca aaaattcagt
     6241 ctgtgagaaa ttcttcgtat cctccctgtc actgaaccga acgaccatct gcgtctttac
     6301 ctcatttctg atcataaccg gcctaaaata aaggctcttg atcttaccgt gcttctccct
     6361 cggtttgctc agcgtaaggg aattaaaact tgaggcttga gttgcttcag ccaatacttt
     6421 caggaattca tcaattttat cagtttgctc catttgactt caaaggtctt gattttttct
     6481 gataaatcag tatttgttgc cactattcag ctacctgtaa atttaatcct gtgcttttaa
     6541 tcagaaaagg caggccggat atacggctca acaccaaagg attacattcg ttttagttct
     6601 tttacctgat taataaataa ctccttttgt tcaccaaaag attctcaggg aatcgcacaa
     6661 agcttttgtt gcagcagacc ggccggcaaa ggaccttagc ggaactttta cgttttgaag
     6721 aaattaaggt ctgataactt atatggtata aggaagaatt agtgctgatt tgaggtggct
     6781 ggttacactt aatcaggcat tcaaaactta tctttgcaat atcaatcaaa tttagccata
     6841 ttgaatacaa gagaaagtga aataaggcac aaccttgctg tcatccatga gcgtatcagg
     6901 aaagcatgca gggactgcaa tcaggcggaa gaagatgtca atctccttct cgccaccaag
     6961 actgtttccc ctgatgatat ccgcatagca gtagaagcag gtgccactct catcggagaa
     7021 aacaaggttc aggaattttc ttccaaggcg gatgctttgg ccgatctctg tattacgaga
     7081 cactttatcg gccatttgca gcgcaacaag gtcaaagaag tccttcgaca cgttcaatgt
     7141 atccagacca tcgacaatct tgcacttgcc gaagaagtaa ataaaagagc aggagcgatg
     7201 ggccgaagtg tggaagtaat gatacaggtc aacacgtctt tcgaagcgag caaatttggc
     7261 atcgcacctg aaaatttgcc ggaattagct gagcaggtaa gaaacatgca aaatcttact
     7321 ctaatcggat tgatgacgat cgggctcaac gccgaggtca ttgaagacgc ccggccttcc
     7381 tatgcattat tgaaggaatt aggcgaaaaa ttaaatgata aatatcccga aaaaccgatt
     7441 cgcgaatact ccatgggcat gagtggcgac cttgaaattg ccatagaaga gggggcaaca
     7501 atcatcaggg tggggagtgc tgtttttggt acaagaattt atccttgaac cggaggatga
     7561 agccgggtgc agttgtcatt gatccccatt gagattccaa ttaccgttcc atttcctcat
     7621 tcaaaacaac aattcacccc tttgagccaa catgctttgt cgaatacatc aaggttattc
     7681 agacaaacga aatgaatgct aaaaattcta tgtgcctcca atttaacttg aataaatact
     7741 atgaaatccc gatttcattt ttaaattaat aatatctttg cacacattca acaaaatacg
     7801 acatgcaatt ttcacttact gaagaacatt tggcggtacg ggatacggcc agggattttg
     7861 cccagaagga attattaccg ggcgtcatag aacgagacag caaaatgatt tttcctacgg
     7921 aacaggttaa aatgatggct gagcttggat ttctcggtat gatggtctct ccggaatacg
     7981 gcggtggtgg tatggacacc attagttatg tattggcgat ggaagaaatc tccaaggtgg
     8041 acaacagctg cagtgtgttg atgtcggtca acaacagcct tgtttgttgg ggacttgagg
     8101 cttttggtac ggaagaacaa aaacaaaaat atctgacccg actggctaca ggtgagatca
     8161 tcggctcctt ctgcctaagc gaaccggaag ccggttcaga tgcaaccagc cagcggacta
     8221 cagcggtcga tatgggtgac tattatctgc tcaatggcac caaaaactgg attaccaatg
     8281 gtggcagcag taagatacat ttagtgatag ctcagacgca tcctgaaaaa ggtcaccacg
     8341 gaatcaatgc cttcatcgtc gagaccgggt gggatggtgt agtcatagga gcaaaagaag
     8401 acaaactcgg catccgctca tcagacaccc acaccattat gtacaacgat gtgaaggtgc
     8461 caaaggaaaa tagaataggc gaagatggat tcggattcaa gtttgcgatg aaggtattgt
     8521 caggaggtcg catcggcatc gcttcacagg ctttgggcat cgcatccggt gcttttgaac
     8581 ttgccactgc ctatagtaag gaaagaaaat cattcggcaa ggaaatatac aaccatcagg
     8641 tcattgcatt taagctggcc gatatggcca cggaaatcga agcagccaga ttgcttgttc
     8701 acagagctgc atggttaaaa gatcagaaaa aggattacaa cacagcctca gccatggcta
     8761 agttgtttgc tgcggaaaca gcgatgaaga caaccgttga ggcagtacaa atccacggtg
     8821 ggtacggttt tgtcaaggaa tatcatgtgg agcgtttgat gcgcgatgcc aagattactc
     8881 agatctacga agggacaagt gaaatacaga aacttgtcat cgcccgcagc gtcattaatg
     8941 gtgaactata tcttccgggc tacgttgtat agtacacaaa tcaaacgctg aatattggct
     9001 atatttgaaa aaggttcgat gtatgccggc acctactttg gtataccggt aaagattcat
     9061 tggactttcg gctacattat cttttacatc atctactcgg gctatcagga caatttgggc
     9121 ttacagggaa tgttatggtt tggcctgttt atcctgagtc tctttctgtg tgtcattctt
     9181 cacgaatacg gccacgcact tactgcccgg agatatgggg tacacaccgc ggatatcatc
     9241 cttacgccct tgggtggcat tgcccgcttg acaaaaatgc ctgacaagcc tgtgcaggaa
     9301 ctcattgtcg ctctcgcagg cccactggtc aatgtaatca tagccggact gctatttgta
     9361 ggattgtact ttggctttga atacaaccca ttcatcattg atgtagacaa tgcctataac
     9421 ttcactggca atccgggcgg ctttttcgga atgctaatgt tgaccaatat agcattggtg
     9481 gttttcaacc tgttgcctgt atttcctatg gatggaggtc gcgtgctgcg tagtctgatt
     9541 gccatgaagg cgagcaaagt ccgcgccacc ctgattgcag ccagaacagg acagctcttc
     9601 tccttatttt ttgtaggtta cggaatctat agcggcaact tgatgcttgc actgatcgga
     9661 ctctttattt ttcagagtgc ctatatggaa tatcgatata ccaaagctga caacctcatg
     9721 caatcctcca tagtcgaaaa cctcggttat gctgaatacg atactttcca gtcagatgat
     9781 ctcatcatca attgtaaaga attaatgaaa tattcagatc agccattttt ccccatcctt
     9841 tccaatgaag ttgtcatagg gagtgtatcc cgggatgaca tcatccaatc cgatttgttg
     9901 tataggcccg tctcagagct gtcgatgatg cctgtacctc agcttcctgc cgataccaac
     9961 tggatgactt gttaccagat attctcaaaa gatcaggccg atgtcattct tttgaataag
    10021 gatggcgcaa aaccaaccct tttggacaaa tggatcttct accagcagtt ggaaaacaaa
    10081 aaactaatca aaaaaggtaa gttataataa attccggtat ggtaatactt atctggaatc
    10141 atttcccaat gtccattcca gcgctttgac ttttgtggct ctgaagatag tattgtgctt
    10201 ctctcctggt tcatcggagt agttcacctt taggttgccc agattggcat tcctgagtat
    10261 ttccgccaat tgcctggtgt gaggatagat gtcctgcacc gaagaacctg caaaccataa
    10321 tcttttttcg gctttcggaa aatgcttcag tgattcggca gcgccattga ccagctggtg
    10381 cttattccac cacagcgatg gatcaaacgc aatataattg tcaaacatat ccggtgccat
    10441 aagaaaagtc tctatgataa acaggcctgc caaagattct ccgatgatcg ttctttgccc
    10501 attggtcctg tatcttgacg ctatttcggg aaacagttcg tcacgaataa acatgcgaaa
    10561 tgccgtggaa ccacctacaa caggcgcaat cttcttatcc cttctgacag ttgtcggacc
    10621 agtcagatcc cgtctccgtt gggtattagg gatgcctacc agaatcgtag ggggtatctt
    10681 tccggcaagt atgagttctg aaagggtgtt tgcaatatga gggaagtcct ctgtgatggc
    10741 gccatccggc atatatagga caggataaga cgctttactg atggcatagc cgggaggagt
    10801 ccagatattg attgcacggg attcgcctaa taattccgat tggatgacga ggctatcgta
    10861 tgggggaata gcatcttgag cctgtccgga aaaggtgatt aaaaaaaata tcattgaaat
    10921 aaaaatacta tgccgcgata ttgtcatcta tcattcattt tatgttaaaa acaaagtaaa
    10981 agtaggtaaa attgaacatt aaatttgttc aaaaaattgc acttcttctg ttcaaaatat
    11041 tttggtttgc tttggtcgat ccgtccggga ctttgagaca caccatcacc gcccaaagaa
    11101 tttgacaaag aactgcccgg taaccagcat ttccggaaac aaccaaacta cttagtattc
    11161 cgaaaagcta ttcttgaaag gaaaagaaga tagtcttaga atataacaac aatccactaa
    11221 attgcttaag ctaaatatca caaaaaaaag cccactttct tgtgggctta ttcttccgat
    11281 tcccggatta ttaattttgt gtggcttctt ccggaacagg agccggcact gtttcttcat
    11341 ttccggcagg agtcccggtc ttcattttct cagccctggc catgcatgtt ttacacaatt
    11401 cattatctcc tatctttctg gctttgaagg catcttctac tgtaccgttg tggatctcgc
    11461 gattcttgat atgctgacaa tcttcaaaga tatggtattt attcccctgg ttggtccagt
    11521 acaccatatc tacaccatta ttaagatctt tcaaagttgc agtttgagcg ttcatttgct
    11581 cggtatactt ctcaattgaa ggcggattaa aatccacacc ggtgatgcct gcaatcaata
    11641 gagctactac gccaaccgcc ccggcaattc ccttggtctt tttatccagg tctttattag
    11701 taaagatcag aataacaagc ggtagaaaag acagaactcc cattatggca cccaattgat
    11761 tctggataaa aaatcttgtc ttatctttct cagaagcggg atccagtcta ttggcccttt
    11821 tccaaagaat cgaacctaca atagatagag ccaggatgcc tacgatcgtc acgattagcc
    11881 aggtgagcat atcatttcgc aaaagcttca aaatggcgaa tacctcacct gctattgcaa
    11941 cgatccaggc aagcatcgca aaaagtcgca attgttttgc ctgacctttt gcttcttcag
    12001 ttgctacgaa ttgtttctgt tctgtcataa tacttttatt tgaattttac ttatatgaaa
    12061 agtaaagata tatacttaaa agaatttatc aaaattttgt tttgaatcaa gcaggaattc
    12121 agattttgta ccgatgttcc ctttgaaaat gtgtctccct gataaagcac ctgtcaacaa
    12181 aatgtaatga agcaatggat caagatgtat ctataaatca aatgcataat ttttaataaa
    12241 tttgtgacaa ttaaatgata atcaatcatg aaaagagcaa tacatatcat actttttttg
    12301 tttggcctta cctctctggt accgcttcag gcccagacca aggtagaatt aaacataagc
    12361 caccttttcg gccaccaacc atttgcccct gaaaatgtcg gtatcaacga ccttaacacg
    12421 cccttcaaaa tagagcgatt ggaatactat atttctgaca ttattctggt ccatgatgga
    12481 ggtgttgaga caaaggtccc ggattattgg attctggcag atatatcaaa aagggggagt
    12541 tacgagttgg gaagctttga catcaaacac ctggaaaaag tcatcttcca tataggcgtg
    12601 gatcccgaac acaacaatgc cgacccttcc gtctatccgg cagatcatcc actggcactg
    12661 aaggagccgg caatgcactg gggatgggca gcaggctata tcttcatagc attgaatggc
    12721 aatgggggag acaaccttga taatcccttc caggttcatg cgctgggaaa tgaaaactat
    12781 tatgctgttg aattggagat gaatattgac gctgtcaatg gcagtcttac aatccccata
    12841 gatgccaatt atgat
//
LOCUS       DAOQQN010000032        12854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1672_length_12854_cov_6.452067, whole genome shotgun sequence.
ACCESSION   DAOQQN010000032 DAOQQN010000000
VERSION     DAOQQN010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12854
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1672_length_12854_cov_6.452067"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PLG24_02145"
     CDS             complement(<1..1038)
                     /locus_tag="PLG24_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ23713.1"
                     /translation="MTRKLIVLLPFFIWTLSLSAQHKTLVDSLYKAFHTQQDPKQKIE
                     LLYLIADEIEESGKPEDIIRYADSLELLSKAANYRKGLSQSIEIRGRYHERKGEHAEA
                     LSFFQKQLNILTSIDDKDGQAKALTNIGNMWHELAANDSAVFYHLKAMKIKEELNKPG
                     DVAISLANIANAYSDLGAYDKAIEMLKRALILRREMGEEKRTMFTLNNLSVAYGRKGD
                     FEHSMAYADTGIHVALKYNNKLVAGVIAGGMGYILNGLQRYKESISWCERSLDYLKEV
                     NRESNMVFPLSNLATAYNGLGRYKEALEQNEKGWAIMQKLSLTEPLEAYQVNFADAYA
                     GLRDYKNALHWY"
     gene            1393..1524
                     /locus_tag="PLG24_02150"
     CDS             1393..1524
                     /locus_tag="PLG24_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23714.1"
                     /translation="MNRWIHMAFGCASPDTSIGEYFNPDAVHFPDEDLDAFETDISD"
     gene            complement(1736..2671)
                     /locus_tag="PLG24_02155"
     CDS             complement(1736..2671)
                     /locus_tag="PLG24_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HMZ23715.1"
                     /translation="MPDKINNPHDKFVRETFSNIDRVTAFFEQFLPEDVRKQLDFTSL
                     KISRESYIQSDLSEYLSDIVFEIKTLREQPLDLVLLLEHKSSPDKYTLIQVGHYLFSH
                     WIKCREKRKSLRPIIPIIYYQGKKKWKAPNIKDLFPGITPSLSEYLPTLNHIFIALNS
                     LSDQEIMTLKISSLAAALFAQKGKFDQSEAFEELKRIFRLLSNSDADRNFILMLSVYI
                     ISVSNIKPPIIIEAMENMSSEIKEKIMTTYEQLIEQGRKEGREQGREEGTKKSKSSVI
                     LNGYDNGLDVAILSNITQLTQEEVIEILRDHGKLA"
     gene            complement(2852..3715)
                     /locus_tag="PLG24_02160"
     CDS             complement(2852..3715)
                     /locus_tag="PLG24_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ23716.1"
                     /translation="MKNVLVFILLILGFSTSGQTFSFPMYFSDALGNKNTLVIGYDIN
                     ASYGIDSAFGEENIITEPMDSVFDIRLTNYMDRNNDHLPVDIHTKKQIIKYHCFTSLY
                     NQIEIDIKAKHWPVTAYWDRTLFSDPCRHWSVFTGVSPGGWWDTGCPSSLDVLNLNSI
                     DSVTFTSNRMNDLNDSYDYVTDGGDTLDVFWITFSHRKIEKGSVKDLRDARNFISVYP
                     NPAGEKINFKLEMPSFNPKRVEIYNSLGQMVLQSDQWVDLDISTLTSGIYFAKMNMWD
                     GMYSVSEFLKL"
     gene            complement(4212..4763)
                     /locus_tag="PLG24_02165"
     CDS             complement(4212..4763)
                     /locus_tag="PLG24_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMZ23717.1"
                     /translation="MKFTLPHTIESGLGETMVFKEIIKDPDSEKVIIEGRCKPTAGPA
                     MHVHLKQDEALTVVKGKMGCQIPGQEPVYYTVGQTATFLRNVPHRFWNAGDDELVISS
                     WVKPVNSIIFFLTVLYAAQKKTGSGRPEMFDASYLMTRYKHEYDMPELPFFVKKIIMP
                     TTYILGRLLGKYKKYEDAPEPLK"
     gene            complement(4891..5361)
                     /locus_tag="PLG24_02170"
     CDS             complement(4891..5361)
                     /locus_tag="PLG24_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23718.1"
                     /translation="MNSKNIFYILACISYCLIIGAAVYEHIAVWPVAFSEPPRSLTMF
                     QGNYAIEPALFWKFIHPVTLTLFIITLGLNWKTERKKFILIPLITYIILLIVTFIYFV
                     PELMSLIKTPYSEAVDEVVRKRGSLWITLSSIRLSLIVITTCILMLGLTKPATK"
     gene            complement(5413..6222)
                     /locus_tag="PLG24_02175"
     CDS             complement(5413..6222)
                     /locus_tag="PLG24_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HMZ23719.1"
                     /translation="METNIYIPTERPLADVVQVFWQEKRENAGFSHEVILPKGIVEIV
                     FNFFPETTFNGKVYDKNFIIPKCFIQGYHNSSIKLDLPRSQFLFGVVLLTAATKHVLH
                     VPAGEFARQCIDLTLVDASFCSLWHQLAEKETFQEKVALFSDWLIKRMTCLSAREQVL
                     NKLFTLNTDKHFSVADISDWLCYSPRHLCRKFYEMTGMNTEQTLLYLKYLKAMNLIHN
                     SDLSLTQIAYSCDFSDQSHFIKVFKSFTNLTPKEYRNKKSVVVGHLFENVR"
     gene            complement(6728..7339)
                     /locus_tag="PLG24_02180"
     CDS             complement(6728..7339)
                     /locus_tag="PLG24_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HMZ23720.1"
                     /translation="MKYSPFNLSEQNYKVESRIVVALERIAEAFRVLLWNESKENSLS
                     PIQIQILIFIYFHSLDKCKIGYLADEFNMTKATISDSVKVLFAKNLVSKHTDPADTRS
                     YSLSLTDKGKKIAQKASFFASSIERPLESLTEEQKTIMLNGLLNLIYSLNKAGVITIQ
                     RMCFNCSNYQVENGVHYCKLLNSRLKESELRVDCPDHELRMHV"
     gene            complement(7462..7860)
                     /locus_tag="PLG24_02185"
     CDS             complement(7462..7860)
                     /locus_tag="PLG24_02185"
                     /inference="COORDINATES: protein motif:HMM:NF021169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HMZ23721.1"
                     /translation="MKQSIILVALVILTSSAIKAQSIIKKETEKKPNTINKNPKNSIN
                     LSKVAVWDTYVTKKDGNVMHFDIIAPQEIKDTNVIYHYGKEYLKTKGQEGQPLTSKEC
                     RFCHIETVRPKWEAEIRQKGYFIIEMENCE"
     gene            complement(7920..8657)
                     /locus_tag="PLG24_02190"
     CDS             complement(7920..8657)
                     /locus_tag="PLG24_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23722.1"
                     /translation="MKHFFLLLGFTLFGLQGFACDKCGNYNNDDFQIVKVNVKHAPDI
                     GATIWEITVKGTAGKTTPTPAGQLNGAPVLGYVFPTTLKPTDVGFGSTEGIVALALTS
                     HPDFDDTPLWDENSDKNYANDGKIWHPHWVVLTEDKRVEGGLAVKQFDPNDNTVILPP
                     TNPGMPMYMDSPGFQVITAGNIIRVVVPDYRMNNKTDFSYDGVAAYMQVNTGGDGGHG
                     GGEKPMLGVYKVYSVASGNLSLPYKVN"
     gene            complement(8737..8970)
                     /locus_tag="PLG24_02195"
     CDS             complement(8737..8970)
                     /locus_tag="PLG24_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMZ23723.1"
                     /translation="MAKSIFYHAGCQVCISAEHEIVNLIGASNVEIVHIGEDRNRIAE
                     AEEAGVKSVPALLTPTGNVLHINFGASMADVKG"
     gene            9781..11619
                     /locus_tag="PLG24_02200"
     CDS             9781..11619
                     /locus_tag="PLG24_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ23724.1"
                     /translation="MKSKIFILILIIANSVFAQNTINIRVDASAETKPMPPIWRDHYE
                     NHLSDGYGGNPAITGNHTLYTTDPSFYTIMKELQPRYIRISIGRIDNPPSVDYSSMNT
                     HTLRNLKYEFYKGGNSTEEANNITNYDFSYIDSAISLIHSFGAEPFITMDYMPFNLSR
                     DTTPEYQGLMGLIYNLAYDNSIRNSPPSDNAVYGRVMYHFIKHCYQKYGVKYFEHWNE
                     PDQQWLNPIMAKFFWRGDEQDLYKAYAAIANEVSSDQSLSPNVKLGGCSFAFYSFLNL
                     MPQNFLTNVQTNGTKFDFLSFHPYSDNQFMGGYDTAKVNLAIQWRNTYVPHAELINAE
                     WGRLEPNSPVWGDLDYGLDKFRHIVDMLDKGISMSHETCLFDGDITTDNFANLGMFRV
                     GPIVPKPSAYVFYNLNRLNDALNRLPVAIDNGGAVLAARSDNGDKILIALPADNPTNG
                     SNAINLTVDNLPWENTGYYLNRYELTEQSYFDGIIFNLTHTRSGAGNTVSDSLSYPSI
                     QNSGRLVLWEISSTPLTVKDLYPEHVILSIYPNPNQGSFSVRSLHENITMKSLTIYTL
                     TGQEVLRKSFPYFQNQININDFIGVGTFIVVIETNRGTYTSRFIAH"
     gene            complement(11994..12296)
                     /locus_tag="PLG24_02205"
     CDS             complement(11994..12296)
                     /locus_tag="PLG24_02205"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HMZ23725.1"
                     /translation="MISYNVSVTIDSEIEQEWVEWMKTIHIPDVMATGCFTEYRFYKL
                     MKPEDEEGLTFIMQYKALTMEDYHRYSTDHAPALQQETHKRYKDRFVAFRTIMVEV"
     gene            complement(12315..>12854)
                     /locus_tag="PLG24_02210"
     CDS             complement(12315..>12854)
                     /locus_tag="PLG24_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23726.1"
                     /translation="GEAVLGRDNEGEVIGEDDRPVVAGETGELVITTLQMEGMPLVRF
                     RTGDLVRRLEEPCTCGRTSARISPVIGRKSQMIKYKGTTVFPSAIQQILDHLPEVETY
                     VIELASDEFGHDRINVMLAIKGEIEGTLNHIRELCKAHIRVTPELMVREASFISHLRN
                     RPEMRKPMVVLDNRLKAHS"
BASE COUNT         3736 a         2614 c         2417 g         4087 t
ORIGIN      
        1 ataccagtgt agtgcatttt tataatctct caatccggca taggcatctg caaagtttac
       61 ctgataagcc tcaagaggtt cggtcaaact gagtttttgc atgattgccc atcctttttc
      121 attttgctcc agcgcctcct tatacctgcc caggccattg taggctgttg ccagattgct
      181 aagcggaaaa accatattgg attcacgatt gacttctttc aggtaatcaa gggagcgttc
      241 gcaccaggag atgctttcct tgtaacgctg aagaccattg agtatataac ccattcctcc
      301 ggcaatgact cctgccacta atttattatt gtacttcaag gcgacatgga ttccggtgtc
      361 ggcataagcc atggaatgct caaagtcacc ctttctgcca taagctactg aaaggttgtt
      421 gagggtaaac atcgtgcgtt tttcctcgcc catttcacgt cgcagtatta aggcgcgctt
      481 caacatttct attgctttgt catatgcacc gaggtcactg tatgcgttgg caatattggc
      541 cagactgatg gctacatcac ccggtttgtt caattcttcc ttgattttca tggccttcag
      601 gtgataaaaa actgccgaat cgttggcagc cagttcatgc cacatattgc caatgttggt
      661 taaagcctta gcctgtccgt ccttgtcgtc tattgaagtc agaatattca gttgtttttg
      721 gaaaaaggac aatgcctcag cgtgctctcc ctttctttca tgatatcggc cccgtatttc
      781 aatggattga gaaaggcctt tacgataatt ggcagccttg gaaagcaact ccagactatc
      841 tgcgtaccgg ataatatctt ccggcttgcc tgattcttca atttcatcag caatcaggta
      901 cagcaactcg attttctgct ttggatcctg ttgggtatgg aatgctttat agaggctgtc
      961 aactaaagtc ttatgctgtg cagataagga aagggtccag ataaagaagg gcagtaagac
     1021 tatcaatttc ctcgtcataa catgttctaa tttgaagcca atcgttttat gaagattgaa
     1081 acaaataaat gacattatct aataacatac cttgaaaata ttcattgaat gtacaaatca
     1141 tttaatcaat agccgtcgca aaaacactat tttatgcctt tacctgtgca aattacggag
     1201 aatttggttt agatgaatat attaatttaa attttttaaa aatcataaat gaaggcctaa
     1261 gcacatcttc acttagatac tcggccgggg tgaccttgca ggaaaataaa accatgataa
     1321 agtgcgatca aataacggat cggggaccta agtgcggtgt tgatatggat gtttttgcaa
     1381 ggcatttata ttatgaaccg atggatacat atggcatttg gctgtgcaag ccctgatact
     1441 tccataggag aatactttaa tccggatgca gttcactttc cggatgaaga tcttgatgct
     1501 tttgaaacag atatttcaga ttaaatagta gctattacaa ataattctac ttcaatcggg
     1561 gataaaaagc attcccatct aacaaatatt tttcaaagga taccggatat tgtagatata
     1621 agatatattc atttatcaga aaatctgtag ggcacaaaag tgaacaccat tctcctgaaa
     1681 cgcccaatct gcatagaatt gcatctacaa aagcgcaact tgaatcaaaa tatgtttatg
     1741 ccaattttcc gtggtctcgc aatatttcaa tcacttcttc ctgtgtcaac tgagttatat
     1801 tgctcaatat ggcaacgtca agaccattgt catagccgtt gagtataaca ctggacttac
     1861 tcttttttgt accttcttct ctaccttgtt ctctaccttc ttttctgcct tgctcaatca
     1921 attgctcgta agttgtcata attttttctt ttatttctga tgacatattt tccatcgctt
     1981 caatgatgat tgggggtttt atgttggaca cacttatgat gtaaacactt agcatcaata
     2041 taaagtttct gtcagcatcg ctatttgata gcaaccggaa gattcttttt aattcctcaa
     2101 atgcctccga ctgatcgaac tttccttttt gtgcaaagag cgcagctgcc agtgaactta
     2161 ttttcagtgt catgatttcc tgatctgata aggaattgag tgcgatgaaa atatggttta
     2221 gtgtagggag atactcagac agagacggcg taattccggg aaacaaatcc tttatgttcg
     2281 gcgccttcca cttcttttta ccctgataat aaatgatggg aatgatgggt cgcaacgact
     2341 tcctcttctc acgacactta atccagtgtg aaaacaagta atgacctact tgaatcaagg
     2401 tgtacttgtc aggcgaggat ttatgttcca gcagcaagac aaggtcgagt ggttgttccc
     2461 ggagagtctt tatttcgaat acaatatcgg aaaggtattc actcaggtcg ctttgaatat
     2521 atgattctct ggagattttc agactggtaa agtccagttg ttttcgaaca tcttcgggta
     2581 agaattgctc aaaaaatgca gtaacacgat ctatattgga aaatgtctct cggacaaact
     2641 tatcatgcgg gttgttgatt ttgtcgggca tatgaatgaa gttttatgag gtaaagatac
     2701 ataaattctt tgataaaagg acaatcctat ccattcatat ttgtagcttt cccatctggt
     2761 taagaaccaa cttcaataaa tgaatatttt ttatatattt ttttctaatg aacctaatcc
     2821 ttcagttcca attatagtga ttaacacatg tctataactt cagaaactca gatacagaat
     2881 acataccatc ccacatattc attttagcaa agtaaattcc gcttgtaagt gttgaaatat
     2941 caaggtctac ccactgatcc gactgaagta ccatttgtcc aagactatta tatatttcaa
     3001 cccttttagg gttaaatgaa ggcatttcta atttaaaatt aattttctct ccggcaggat
     3061 ttggatagac ggaaataaag ttccgtgcat cacgaaggtc ttttacacta cctttttcaa
     3121 tcttcctatg actgaaggtg atccaaaaaa catccaaagt atcccctccg tctgtaacat
     3181 agtcataact atcattcagg tcattcatcc gatttgaagt gaatgtaacg gaatctatac
     3241 tatttaagtt caagacatcc agactactcg gacagcctgt atcccaccag ccacccggtg
     3301 acactccggt aaaaacactc caatgcctac acggatcact aaataacgtg cgatcccagt
     3361 atgccgtaac cggccaatgc tttgctttga tatcaatttc aatttgatta tatagcgatg
     3421 taaagcaatg atattttatt atttgctttt tagtatgaat atcgacaggt aagtggtcat
     3481 tattacgatc catgtaattg gtcagcctga tgtcaaaaac cgagtccatt ggttcggtga
     3541 tgatattctc ttctccaaaa gcagaatcaa taccataaga tgcatttatg tcatatccga
     3601 ttaccaatgt atttttattt cccaaagcat ccgagaaata cattggaaaa gaaaaagtct
     3661 gtccagatgt tgaaaaacca agaatcaata aaataaaaac aaggacattt ttcattggaa
     3721 ctattttaat gatcagtaat gtgcaatgac aattcagata tgaaagctct tatttgtaca
     3781 agtgtaaata aagttttaat aaaaaccaag atttacttaa aatatttacg gtcagaactt
     3841 tatggcgaaa tgtaggataa aaatgttaaa tcaagatgat ttagataata gttctattat
     3901 tttggtataa gggaaagggt aaagttcttt aaagtaataa aatgagtctc tttcaattgt
     3961 acatacggta ctgcctaatg aaaggatctt atacaagcaa aaaatatacg aactacgaag
     4021 cctcaaagtt ttacctcata gtcgtcccta gaaaaaacat attaacttct ttttaaacaa
     4081 taaaagataa aacaaaaagc tattaaaata atgcaattta tgagtaaacg atgtgtcacc
     4141 ttgatccaca aatctgaatg tccacaataa acatgaccga ttgttattac atttaagtac
     4201 ctccgggcgt cctatttcaa tggctccggt gcatcttcat attttttata cttgccaagt
     4261 aaccgaccaa gaatatatgt tgtcggcata atgatctttt taacgaagaa ggggagctcc
     4321 ggcatatcgt attcatgctt atatcttgtc atgagatagg aggcatcaaa catctccggt
     4381 cgcccgcttc ccgtcttttt ttgtgccgca tataggacgg tcaaaaagaa aataatgcta
     4441 ttaaccggct ttacccaact gcttattaca agttcgtcat cgcctgcatt ccagaagcga
     4501 tgcggaacat ttctgagaaa agtggctgtc tgcccgaccg tataataaac aggttcctgc
     4561 cccggaatct ggcaacccat ttttccttta actacggtga gagcttcatc ttgttttaaa
     4621 tgaacatgca tggcgggtcc ggcagtgggt ttgcagcgtc cctctatgat cactttttca
     4681 ctatccgggt ctttaattat ctctttgaag accatggtct ctccgagtcc gctttcaatg
     4741 gtgtgtggca gagtaaattt catttttatt tatttaagtt agatcaaatt ttaattccta
     4801 ccaaataatt caaatgaggg gtcaaaataa gccttagcac atcgttttaa ttcttttgat
     4861 gtatcagtga gacctcttgg gtcaatgata tcattttgtt gcaggtttag ttaaccccaa
     4921 catcaatata caggtcgtga ttacaattaa ggacaatcgt attgagctca gtgtaatcca
     4981 cagactgccc ctcttccgga cgacctcatc aactgcttca gaataaggcg ttttgatcag
     5041 gctcattagt tccgggacaa aatatatgaa cgtaacaatc aacaatatga tataggtgat
     5101 cagcggaatt aagataaatt ttttcctttc ggttttccaa ttcagtccta aggttataat
     5161 aaataaagta agggttacag gatgaatgaa cttccagaat aaagccggtt caatggcata
     5221 attgccctga aacatagtaa gtgatcgggg cggctcggag aaagccacag gccatactgc
     5281 tatatgctca tagacagctg cgccaattat caggcaataa gatatacacg ccagaatgta
     5341 aaatatgttt ttgctattca tgccggacat tttttagtaa aaatgccaat ggtctgttta
     5401 gcaaaattgt atttaacgga cattttcaaa aagatgtccg actactacgc ttttcttatt
     5461 ccgatattcc ttaggggtga ggttggtaaa tgacttaaag actttgatga aatgagattg
     5521 gtctgaaaaa tcacaggaat atgcaatttg cgtaagtgac aaatcagaat tatgtataag
     5581 attcatggct ttgagatact ttaaataaag caatgtttgc tcggtattca ttcccgtcat
     5641 ttcataaaat tttctgcaaa ggtgccgtgg cgaataacac agccaatcag atatatcggc
     5701 aacagaaaaa tgtttatccg tattcaatgt gaataattta ttgagaactt gctcgcgtgc
     5761 agacaggcag gtcatgcgtt ttatcaacca atccgaaaaa agagctacct tctcctgaaa
     5821 ggtttccttc tctgcgagct gatgccacaa tgaacagaat gaagcatcca ccaatgtcaa
     5881 atcaatacat tgtcttgcaa actcaccggc agggacatgc aggacatgtt tggtagccgc
     5941 cgtaagtaaa actacaccaa aaagaaattg gctgcgaggc aggtctaact tgatagaact
     6001 gttatgatat ccctgtataa agcatttggg aatgatgaaa ttcttatcgt aaactttgcc
     6061 gttgaacgta gtttcgggaa agaaattgaa gacgatttca acaattcctt taggaaggat
     6121 gacctcatga ctgaatccgg cattctctct tttttcctgc caaaagactt gcaccacatc
     6181 agccaatggt ctttctgttg gtatataaat attagtttcc atactaccaa atatcttatc
     6241 atctcgttaa gccattatgg ctaacgtatg accaacatgg ctcacaggca gaatttccac
     6301 tgaaaattcc gggtgagcga caaagtgaaa aaatccggca aagtcttcaa acggaaactt
     6361 ttcccgcctg acacaaagcc cgtgtaactg ttgtacaaat caaatttaca atattaaggg
     6421 taattgccgg gtatgaaata acaggaaaat atcatttctt aatggattgg tgcaaggaag
     6481 attaaggcct tatctaattt gcaggcattg tccgggacga tttcgtccaa cgaaacttga
     6541 tatctgcacc ttgcattttt ccttaaccgc tcgaaacagc gctgatcctg gtcaaatttt
     6601 tcttcacgca gcaatatctc taagccttca cataaagata agttttggtg ttgtctggtt
     6661 tctacaagag atttccaaat tcggctctta cctcgcagga atatttcatt ggcaatattt
     6721 cgaaagatta aacgtgcatt cttaattcat ggtcaggaca atcaactctt aattcgcttt
     6781 ctttaagccg gctatttaaa agcttgcagt aatgaactcc attctcaacc tgataattag
     6841 aacaattaaa acacattcgc tggatagtaa taacgcctgc tttgttcagg ctgtatatca
     6901 aattgagcag cccattaagc ataatagttt tttgttcctc cgtgagactt tcaagaggcc
     6961 gctcaatgga tgaagcaaaa aaggacgctt tttgagctat tttttttcct ttatcggtca
     7021 gggaaagaga atagcttctg gtgtctgccg gatcagtatg tttggagact aaattcttgg
     7081 caaacagcac ttttacacta tcgctgatgg ttgccttggt catattaaat tcgtctgcca
     7141 ggtaacctat tttgcattta tctaaggaat gaaaataaat aaagatcaaa atttgaattt
     7201 gtattggact tagtgaattt tctttgcttt cattccagag caatacccga aatgcttctg
     7261 ctattcgctc caatgccact acaatacggc tttcgacctt ataattttgc tcgcttaaat
     7321 taaatggaga gtatttcatg ctttttgata ttatgcaaca taaatcttac aatgtaccct
     7381 ggtttctact tacatcattt gcgtttgtat agcgggtatt tgccattatt tctaaatgat
     7441 tttggccccc ggagcctgtt tttattcaca attttccatt tctatgatga agtatccttt
     7501 ttgcctgatt tccgcttccc attttggcct gacagtttca atgtggcaaa accggcattc
     7561 ctttgatgtc aggggttgtc cttcttgtcc tttggttttt agatattctt tgccataatg
     7621 ataaatcaca ttagtgtctt taatttcctg gggagcaatg atgtcaaagt gcatgacatt
     7681 gccatctttt tttgttacat aggtgtccca gactgctact ttactcaaat ttatagaatt
     7741 tttagggttt ttattaatcg tattgggttt cttttccgtc tcttttttaa taatagattg
     7801 agccttgata gctgagcttg tcagtatgac gagagctact aatataatac tttgtttcat
     7861 tttaaatagt tgtatgccgt gtaaagatag gactcctatc tttacacggc aattttaaat
     7921 taattaactt tatacggtaa ggacaaattt ccgcttgcta cggaatacac tttgtataca
     7981 cccagcattg gtttttcgcc accaccatga cctccatcac cgcccgtatt tacctgcata
     8041 taggccgcaa caccatcata agaaaaatct gtcttgttgt tcattctgta atccggaaca
     8101 actaccctga ttatatttcc ggcagtgatg acttgaaatc ccggagaatc catatacatt
     8161 ggcattcccg gattggtcgg cggtaaaatc accgtattgt cattggggtc aaattgtttg
     8221 actgccaaac ctccttctac ccgcttgtct tctgttaaaa ctacccaatg cggatgccag
     8281 atcttgccat cattggcata gtttttatca gaattttcgt cccaaagtgg ggtgtcgtca
     8341 aaatcaggat gtgaggtaag ggcaagcgcc acaatacctt cagtggagcc aaagccaaca
     8401 tctgtaggtt ttaatgtagt tggaaataca taacctaata ctggggcccc attgagttga
     8461 cctgccggtg ttggcgttgt ttttcctgct gtccctttga cagtaatttc ccaaatggta
     8521 gcaccaatgt caggagcatg ttttacgttt acttttacga tttgaaaatc gtcattgttg
     8581 tagttgccac acttgtcgca ggcaaagccc tgtagcccaa aaagagtaaa tcctaataga
     8641 agaaaaaaat gtttcatttt attgaattta aatggtaaaa gtataagtta gggagttcat
     8701 aaaattagga atcctaacaa atctgattaa atttttttag cctttgacat ctgccataga
     8761 cgctccaaaa ttgatgtgca acacatttcc ggtcggtgtt aagagagcag gaacagattt
     8821 tacgcctgcc tcttcagctt ctgcaattcg gtttctgtct tctccgatat gaacaatctc
     8881 aacattggat gccccaataa gattgacgat ttcatgttct gcactgatac acacttgaca
     8941 accagcatga tagaaaattg atttagccat ttgttttata atttaaagag ttatttggca
     9001 ttattgccac tgcaaatata gtaaggaatc ctaattaaaa aaacttattg aacatttttt
     9061 ttgaaaaaca caaattatcc gataaccgct ctgatttgat tgtaatgttt gatttatcag
     9121 ctttttgaat tcgattttaa agtctttatg cttgttgcag gctattgtat gtattgaaaa
     9181 attttcaatt atacttcctg ttttttcccg gaagtgtttc gaaattttcc tggctttaac
     9241 ttccagtttt tctttgtgag tcataccgga agattttatt gtgatgctat caaggcttcc
     9301 ggtttttgct gaattctcat catccgatgt tcgcatactt ctatacaata atctacgctc
     9361 ttcttgttcc gtaagtttta cccatcctct cattgattaa atccataatt acaaaacgga
     9421 agagcacatg gtttctttcc gtaacagaaa aatatttatt cattttctgc gagattcctt
     9481 ttccggttct tattcccgaa acagttttat tgttgtatcc atgattattt gtatttgtgt
     9541 aaaactattt ttaggaatga cctacttgac ttcatccctt gatggtatta attgtagcgg
     9601 attgaaaaac aaatcattgg atggtccata gtttgctgaa atgtaaatat ttggaagttt
     9661 gaagttgaga acttgtcagg aaaatattcg tatttttgaa ggtcaatgaa atgattgttc
     9721 gctgcaagct ccgacctgtc aggaggcagt tgtcagaaga tattgataat tgttcaacgg
     9781 atgaaaagca agatctttat tttaatactt ataatagcaa attctgtttt tgcacaaaac
     9841 acgatcaata tccgtgttga tgcaagtgca gagacaaaac cgatgccacc tatctggaga
     9901 gatcattatg aaaatcacct ttctgatggt tatggaggga accctgccat caccggcaat
     9961 catacgcttt atacaacgga tccttctttt tatacaatca tgaaagaatt acaaccaagg
    10021 tatatccgga tatcaatcgg aagaattgat aatcccccca gtgttgatta ttcatcgatg
    10081 aacacccata cactgcgtaa tttaaaatat gaattctata agggaggcaa cagtactgaa
    10141 gaggcgaata atatcactaa ttatgatttt tcatatattg attctgccat ctcactgatt
    10201 cattctttcg gagcggagcc gtttatcacc atggattata tgccctttaa tttgagcaga
    10261 gacactacac cggagtatca gggactaatg ggactgattt acaaccttgc ctatgataat
    10321 tccattcgaa actcaccacc ttcggataat gctgtctatg gaagggtgat gtatcatttt
    10381 attaaacatt gttatcaaaa atatggcgta aagtattttg aacattggaa cgaacccgac
    10441 cagcaatggc tcaatccaat catggctaag tttttttgga gaggagatga acaggatctc
    10501 tataaagctt atgctgccat agccaatgag gtgtcctcag atcaatcact ttctccgaat
    10561 gtaaagcttg gcgggtgcag ttttgccttc tattcgtttt tgaatctaat gcctcaaaac
    10621 tttttaacta atgttcaaac caacgggaca aaatttgatt ttctttcatt tcacccatac
    10681 tctgacaatc aatttatggg agggtatgat acagcaaagg taaatttggc aattcaatgg
    10741 agaaacacct atgtgccaca tgccgaactg attaatgctg aatgggggcg actggaacca
    10801 aactcgccgg tgtggggcga cctggattat ggattggata agtttcggca tattgtggat
    10861 atgcttgaca aaggaatttc aatgtcccac gaaacatgtt tgtttgatgg ggatatcaca
    10921 accgacaact tcgcaaatct tggtatgttc agagtgggtc ctattgtacc caaaccgtcc
    10981 gcttatgttt tttataattt aaataggttg aacgacgcac tcaacagact ccctgttgcc
    11041 atcgataatg gaggcgcggt attagcagcc agatcagata atggagacaa aattctgatt
    11101 gccctacctg ccgacaaccc tacaaatggg agtaatgcaa ttaatcttac ggttgataat
    11161 ctgccatggg aaaataccgg atattatctt aaccgatatg aattaacaga gcaatcttat
    11221 tttgatggca ttatattcaa ccttacgcat acccggtcag gtgccggcaa tacagtatct
    11281 gatagcctta gctacccttc tatccagaat agtgggcgcc tggttctttg ggaaatttca
    11341 tcaacaccat taacagtcaa ggatttgtat cctgaacatg taattctttc tatttatcct
    11401 aatcccaatc aagggagttt ttcggtcaga agtctgcatg aaaatataac aatgaaatct
    11461 cttacgattt acactttgac cggtcaagaa gtcctgagaa agagttttcc atattttcaa
    11521 aatcaaataa acatcaatga ttttatcggt gtcggtactt tcattgtagt catagagacc
    11581 aataggggta catacacctc cagatttata gcccattgat tttgtagtaa agtaatatca
    11641 aatgttcatt cgtaacagac cattttcatt ggtcaagatg actgtaaccc cggcatcccg
    11701 gaaccccgga ttgcttcact cccgggacca gtattcatgg attttatgga caattatata
    11761 aattatcttg gtattacacc acaagcatgg caataccaca aagggagtca tcccttttct
    11821 gtgacgttag cacttcccct tgagctaagt gactttcccg ggaaattatg gagccaacat
    11881 ttatgactcc ttgtacaatg agttatatac tcaacctccg gggacattca ttccacagat
    11941 actctatagc tttttcagtc ttcgaattga ttcctctttc gtgtcgcctt ctcttacact
    12001 tccaccataa ttgtcctgaa cgccacaaat cggtctttgt aacgcttgtg cgtctcttgt
    12061 tgcagggcgg gagcatgatc tgtactgtat cggtggtaat cctccatcgt cagagccttg
    12121 tactgcatga taaaggtcaa tccttcctcg tcctccggct tcatcaactt ataaaacctg
    12181 tactctgtaa agcatcctgt cgccattaca tcaggaatgt ggatggtctt catccattct
    12241 acccattcct gttctatctc gctgtcaatt gtgacagaca cattataaga aatcatattg
    12301 ctctttgtta ttttttatga atgagccttc aggcggttgt cgagcaccac catcggcttg
    12361 cgcatctccg gcctgttgcg cagatgggag ataaaggacg cctcgcgcac catcaattcc
    12421 ggtgtaacac ggatatgtgc cttgcaaagt tcccggatgt ggttgagcgt gccctctatc
    12481 tcgcccttga tagcgagcat tacgttgatg cggtcatgac cgaactcgtc cgaagcaagc
    12541 tcgatcacat aagtctccac ctcgggtaaa tggtccagta tctgttggat ggcagaggga
    12601 aagaccgttg tacctttgta tttgatcatt tggctttttc ggcctatgac ggggctgatg
    12661 cgggctgagg tacgtccaca ggtacaaggc tcctccagcc tacgcactaa gtcaccggtc
    12721 cggaaacgca ccagaggcat gccctccatc tgtagtgtgg taatcaccag ttctccggtc
    12781 tcaccggcca cgaccggtcg gtcatcttca ccgatcacct cgccttcgtt gtctctcccg
    12841 agcaccgctt cgcc
//
LOCUS       DAOQQN010000033        12564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1734_length_12564_cov_6.192741, whole genome shotgun sequence.
ACCESSION   DAOQQN010000033 DAOQQN010000000
VERSION     DAOQQN010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12564
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1734_length_12564_cov_6.192741"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PLG24_02215"
     CDS             <1..861
                     /locus_tag="PLG24_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HMZ23727.1"
                     /translation="TINITQGTSPDTVTKFTRIAMASGGPLARTAEDLELAWKVLKST
                     KPDDRFQQPFQWKPKTNKQLDQYKVAWMDEWKHGNKTFKLNAEVKNIIIKLADSLKQN
                     GVQISKTAPDTYGEMKKLFYGSFASMMSEGMPWLLRKFMAMQFKSWDDDKIGYQALKD
                     GFEDNSDQKWQELQKDRERLVTEWEAFFGQYDFLICPVAYGPAIKKCPPGSALPVDNG
                     TMPYFKYTSPAFIINATGHPSITVPMGLNKEGVPIGVQIVSAYYNEDELLHFAKLISK
                     FTPGFIKPSK"
     gene            1361..1852
                     /locus_tag="PLG24_02220"
     CDS             1361..1852
                     /locus_tag="PLG24_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23728.1"
                     /translation="MKNSLILLTIFMCATSTIAQKSKFSVFPNFGVTKPILDGGLGFH
                     AGVNPSYKLSNRFALEGQVSFSYTDISSAFLSGKRGSVSASNFLTGGRLYLNPNSKKS
                     IFYLNLLAGLNVIKEKSEGLDLGTEYGFGFSTGGFVNLSGLTLGITYDTPQNLVLKLG
                     LSL"
     gene            2677..3819
                     /locus_tag="PLG24_02225"
     CDS             2677..3819
                     /locus_tag="PLG24_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23729.1"
                     /translation="MKKNKVILFLTALFVLCNQGHTNAQQGIRGYLLGEYAISASWVN
                     SHLTLLDYTTLQRETTELLTSKVLLNANNMLIAKTWDSTLVNYQIYIINNIINSARLS
                     SGMYGNNGNPDDYLGWMEFADGIEKPLYESSPLGYIVQFLYMLQENGWKDKLPENKIW
                     WERVLNFVEIHEWKKWGERSYYKYAHYIRKRNYMGANWAIVALYLKKLSSNQELITQY
                     NEVLDIYNTLLKRNLKPNPNHPDAYIWNSSWDDLSGIWVQGITPDTVDLQDVAHANSI
                     INYIIAAYETGEADWTIVDIQRMCNTAKNVIYDPVTNNFVRNIDGTDIDLNNGSSAFG
                     NVGDGWVKLSKYDPGVKAVMKEFVKNKDKYPYLYQPILFAAYFYGE"
     gene            3851..5803
                     /locus_tag="PLG24_02230"
     CDS             3851..5803
                     /locus_tag="PLG24_02230"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HMZ23730.1"
                     /translation="MNRIFGFKKWTKLVTIVLFMIFWLKSDGVYSQYNFFSPVHVGGK
                     EFKKLKNSAVAVGDVNNDGLPDVFLCGQNTQGFASSSIYLNEGAAGFAEMNIGNAPLE
                     GVIYGGGAFGDSDGDGFPELLLFGWNLNKGEPRTYLYKNNGGQSFAEMNIALPGIISG
                     FALFGDIDGDGDLDLIIGGISKTYEFITNMYKNDGSGNFSLDNSTSLPQLGRSQAVLA
                     DLNNDGHLDLVITGLDNRYNPAAFVLINDGNGGLVVKPQTELKGVYQGSVSVADVNGD
                     GFSDLFLTGSTKYSGRLSALYINDGTGSFSQSNLSTFNNVWISSTQFADVNKDGHMDL
                     IMTGSDLNYQSVIHLYLNNGLNAFSEVQQTNLEGEIWGDLIMQDFDKDGDPDLIITGE
                     NYRYEGSAVYYSNTSELLPEAPVVNLGADTILCRGQHENFVLEAGSDSSGNKILWNTG
                     DVSDKIVVDTSGTYSVVVTNIYGQSSIDSITVWFKDSPELNGIVISKLDSLTFDFEAE
                     GANIEEFYWNFGDGSISTESNPSHKYELSGEYIVLLIASNPCGRDTVIQNLSIILSTN
                     DISRHSRVRMYPNPSSGYLNLHSAGSPISTINVINIAGTLLINQPVAPSAKYTLDLSV
                     LEAGNYFVRITLSDGSVFNQKLSIIR"
     gene            6317..7408
                     /locus_tag="PLG24_02235"
     CDS             6317..7408
                     /locus_tag="PLG24_02235"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMZ23731.1"
                     /translation="MARGKKLIWLQIILGVAFLLQPILLPVRPDDMYRFVFSKEILKN
                     MLANVLILVFFYWNYYFLIPGFYLAKKYLPYFLIVILSFSTILFISNSVTSQISPAKQ
                     GNRAFPVIEFRNNHPQFGFRQIPTPQGSFLSKLRFFFTENDQIFFLFGSIVLFSLLLQ
                     MSNRYYKTENAKQEAEINYLLAQINPHFLFNTLNSIYTLAIIENATKASTGLLKLSGL
                     MRYIITETHQNSVPLEKELECINDFIDLQKLRIGNHVKLDYDVSGKMSGKQIAPMLLI
                     PFIENAFKYGISPDEDSAIQISIHVIDNVLTMKVSNNKVAINNASTENSGLGIENAKN
                     RLNLLYPNKHDLSISETEKKFNVTLTITC"
     gene            7408..8094
                     /locus_tag="PLG24_02240"
     CDS             7408..8094
                     /locus_tag="PLG24_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HMZ23732.1"
                     /translation="MLRAIAIDDEPLPLELLRAFCSQTDIISLEKTFSKVNEAQLYLQ
                     NFEVDLLFLDIQMPKLSGIELYKRLEQDTMVIFTTAHSQYAIDGFNLNAVDFLLKPFS
                     YERFLVAINKANDYKNYRLNKESSDIQHLYVRSDYNLNKISVADILFIESFADYLDIH
                     IADNKKITTRMSLKSIMEKLPQKDFRRIHRSFIVSIKHIEKMNRSSIKIANRKIPIGT
                     SYKDDYYKLF"
     gene            8230..8718
                     /locus_tag="PLG24_02245"
     CDS             8230..8718
                     /locus_tag="PLG24_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ23733.1"
                     /translation="MKNTVLAGALFFLFGAGFATAQTPVKVTLLDNTEQNYTVESTGK
                     IWFSGSNLVINTDNTLTPTTIPLSNIRKITFDGSGNTNAVNEVTKNKETISIFPNPTN
                     DYFDIEAEGASSLSVYIYNTKGSQVAKGIYKSGERVNVSNLIPGIYVVVINNQSFKLI
                     KI"
     gene            8715..10847
                     /locus_tag="PLG24_02250"
     CDS             8715..10847
                     /locus_tag="PLG24_02250"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HMZ23734.1"
                     /translation="MKKISLFLSLILLVLSIQEITAQNILRVHNSDHLIYQQKVENID
                     SLKLGNNNSDFFPNTGMFALPIAGIDSITFANNTDDEIYIIYNGSSATIINPFSTQGV
                     SIKDSLGHVTATSTYPSSGLKYHILGTTTNGSLKITSAQSVRLIMSHAAVTNPGGAAI
                     SINGDSEMYLSSGTVNTLSDATTASTSGALYVKGNLSISGSGTLNVNGYKKHAILVDA
                     TLVIESGIINIAEAASDGIHVNDYTQNGGTITIIPTGDGIDVSNALILNDGELTVEAA
                     SDDVKGLKATTVTINDGTSNITVSGAQSKAIKSSGTTTINGGTTSVTASGSVVLEASG
                     SGFDPSYCTGIKSDVDVIINNGTLTIQCLASNSGGKGISTDGNITINGGSVNVSTAGN
                     GATYTDENGVIDSYTSTGLKADGNILLLAGNITCSSSGTGGKGVSADGTITIGNPGAV
                     DDLLVLNVTTSGNRFYVSGSGQNADYANPKAIKAEGDLTVNSGLITVNCTQNTEGGEG
                     LESKANLYIKGGQITATTYDDCVNAATHIEVSGGTHSLTARGNDGMDSNGTLTISGGL
                     IVSKGAGGPEEGFDCDNNTFKVLGGIMVGTGGNTSNPTTGVSTQNSLKLSITPNQNIC
                     IKNAANQTILIYALPALTGGGGPGGNNKMVMLFSDPAFVNGTYTIQYGGTISGGTNFN
                     GYYTGATYSGGSSQTFTVSAKYTTVNVP"
     gene            11474..12067
                     /locus_tag="PLG24_02255"
     CDS             11474..12067
                     /locus_tag="PLG24_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HMZ23735.1"
                     /translation="MKRSVVILIVVAVIALIAYSSFKGGYNNMVTKEEAVEAAWSQVE
                     NVYQRRADLIPNLVNVVKGAANFEKETLTGVVEARAKATQITVDPKNLTPEKIQEFQA
                     AQGELSQALGKLLMITENYPELKANQNFRDLQVQLEGTENRITQERRKFNEVVQDFNT
                     YIRQFPRNLIAGMFGFEKKGYFEADKGAEKAPTVDFN"
     gene            12101..12535
                     /locus_tag="PLG24_02260"
     CDS             12101..12535
                     /locus_tag="PLG24_02260"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HMZ23736.1"
                     /translation="MSRLFNPEQEKRIVEGITKLESNSTGELRIFVEDSCNGNLHDRA
                     IEVFVEHGMQHTLNRNAVLIYIAVRSRKIYVWGDEGIHAKAGQGLWDQILADLTADFK
                     DSMFEAGILRAIEAIGSKLAEFFPRDGTEGNNELPDDIIYGF"
BASE COUNT         4028 a         2306 c         2584 g         3646 t
ORIGIN      
        1 actattaaca ttacacaggg aaccagcccc gatacagtga ctaagtttac aagaattgcc
       61 atggcatcgg gtggtccatt agctcgaacg gcagaggatc tggaattagc ctggaaggtt
      121 ttgaaatcta ccaaaccgga tgaccgtttt caacaaccct ttcaatggaa acctaaaaca
      181 aacaagcaac tcgatcaata taaggtagcc tggatggatg aatggaaaca tggaaataaa
      241 acatttaagt tgaacgctga agtaaaaaat ataatcatta agctggctga ttcactaaaa
      301 caaaatggcg ttcagatttc aaaaacagca cccgatacct atggcgaaat gaaaaaactt
      361 ttttatggat ccttcgcctc catgatgagt gaagggatgc cctggttgct acgtaaattt
      421 atggcaatgc aattcaaaag ttgggatgat gacaagattg gctatcaagc tttgaaagat
      481 ggttttgaag ataattcaga tcaaaaatgg caagaattac aaaaggatag agagaggctt
      541 gttacagagt gggaagcctt tttcggtcag tacgattttt tgatttgtcc ggttgcctat
      601 ggtccggcaa taaaaaaatg cccaccaggg tcagcacttc ctgtagataa tggcactatg
      661 ccgtatttca aatatacttc tccagcattc atcattaacg caacagggca tccatccata
      721 acagtgccta tgggtttgaa taaggaaggg gtaccaattg gtgtgcaaat tgtcagtgcc
      781 tattacaacg aagatgaatt gctgcatttt gcaaaactca tttcgaaatt tactcccgga
      841 tttattaaac catctaaata atctttagag tattagacag acgataaaga gaaaaagata
      901 gtatgaatgc gcaacagcta aaggtttaat cacttccggc gcagcgaatt tgttttgact
      961 cgcagttatt aacccggcca ttctcaggat ggtctgtagc accaggctcg accggtttaa
     1021 tggcattgat gcatcctgaa tcttcttatt tgaatgtaat cttagtgaaa gcctttgtga
     1081 aaacacagga tcagtactgt atgtatacta aagaggattg gtccggaaat gtggagcagc
     1141 gatgtggtag attaacaggc gattggcaaa gaggcggatg atgtaaaaat agaactttta
     1201 atattttttt atcacccaaa atgatacatt attcattttt tttgtatgtt cgtattatga
     1261 aaacatatcg gccacatttc gtatagattt aatttcccat ctaatttatc tgctgaatcg
     1321 ccaatgtgtt tactattgta aacaattaaa aaatatgaaa atgaaaaatt cattaatcct
     1381 tctcacgatt tttatgtgtg ctacaagtac tattgcacaa aaatcaaaat tttcagtttt
     1441 tccgaatttt ggagtaacaa aacctattct cgatggtgga ttaggatttc atgcaggggt
     1501 gaatccttcc tataaattga gtaataggtt tgcactggaa gggcaagtgt ccttttcata
     1561 caccgatatc tcatcagcgt ttttatccgg aaagagaggt tcggttagcg ccagcaattt
     1621 cttaacagga ggcagattat acttaaatcc aaattctaag aagtcaattt tttacctcaa
     1681 tttacttgcc ggtctgaatg taataaaaga aaaatcagaa ggtcttgacc tgggaactga
     1741 atatggcttt gggttttcaa caggagggtt tgtaaacctt agtggattaa cattgggaat
     1801 aacttacgat actccacaga atctggtttt gaaattaggg ttatccctct aaaatcaaac
     1861 aaaagaacaa cgggctacaa taaagactat gatgtgccat gctgccccgg agtggtatgg
     1921 catatcattt agtcagcgtt gacatccatt gagaaatgca accaaatatt aacccgggtt
     1981 ccgcattgtt caacaaattg tgttaaaaaa cggtcaaaga acagcaaatt ttgctccaga
     2041 acggccaatt ttaattagat ataaaacata aaagacggat aatcaatcgt ttttgtgttc
     2101 cggcttctca aaaacggcta tgtagtactc aggggggtgt tgttggtatg ggaagcaatg
     2161 ccgccctttg cagtatgtat tttaagcgtg ttgttcgtat caatcctgtt acgggacaac
     2221 cggcaggtta ataccggttg gtatagaagg tgattcgtaa ttgcccggct ggggagaaga
     2281 tcgcattagc caggtgtcaa acactcatgg acatagaccc ttcggatagc aagaacacaa
     2341 aatgtggtta caacaagagg ctctaataac gaatgtacct tcggcatccc cgaaccccgg
     2401 attgtttcac tcccgggaca gagcagagct ccggtagcat agctgtcccg acaagtatac
     2461 caaaaaatta tgaactatta tataaatcat cctgggataa cattgaaaat aaaatcaagc
     2521 acatacaaat acccttgaca ggtatgccta taccaaacaa tcctaaaatc agaaggagat
     2581 cgcattttga aataaacttt tttattttta tatttgaaac tgaacaacag agaaaccaaa
     2641 actgccatta atttttgatt atacattgtt catgatatga agaaaaataa agtcatactt
     2701 tttttaacag cactttttgt attgtgcaat caaggccata caaatgctca gcaaggcatc
     2761 agaggatacc ttctgggaga atatgccatc agcgcaagct gggtcaatag tcacctcacg
     2821 ttgcttgact atactacatt gcaaagagaa accaccgaac tcctgacctc taaggtatta
     2881 ctcaatgcca ataatatgct cattgcaaaa acatgggatt caaccctggt caactaccag
     2941 atatacatca taaacaacat tattaattcg gcccgcctca gtagtggaat gtatggcaac
     3001 aatggtaacc ctgacgatta ccttggctgg atggaatttg cagatggcat cgaaaaacca
     3061 ctttatgaaa gttcgcctct cggatatatt gtacagtttc tgtatatgct gcaggaaaat
     3121 ggatggaaag acaagcttcc tgaaaataaa atctggtggg agcgcgtatt gaattttgtt
     3181 gaaattcatg aatggaaaaa atggggcgag cggtcgtact ataaatatgc gcactatatc
     3241 aggaagagaa actacatggg ggccaattgg gcaatagtgg cattgtatct gaaaaaattg
     3301 agttccaatc aggaactcat tactcaatac aacgaagtac tggatattta caatacgctc
     3361 ctgaaaagga atctgaaacc taacccgaac catccggatg cttatatatg gaacagcagc
     3421 tgggacgacc tgtctggtat atgggtgcag ggcattacac ccgataccgt cgatttgcaa
     3481 gatgtggcac atgccaactc aatcatcaat tatatcatag cagcatacga aacaggagag
     3541 gcggactgga cgatagttga catccagcga atgtgcaaca ccgccaagaa cgtcatttat
     3601 gatcctgtaa caaataattt tgtcagaaac atagatggta ctgatattga tttaaacaat
     3661 ggatcaagtg cctttggtaa cgtgggcgat gggtgggtga aattatcaaa atacgatccc
     3721 ggagtgaaag ctgtaatgaa ggaatttgta aagaataagg acaaatatcc ttatctgtat
     3781 cagccaatat tattcgcagc atatttctat ggagagtaaa ttgtttttta aaaaatttag
     3841 attcggtaaa atgaacagaa tatttggctt taaaaaatgg actaagcttg taacgattgt
     3901 tcttttcatg attttttggt taaaatctga tggagtgtac agccagtata atttttttag
     3961 tcctgtccat gttggcggca aagaatttaa aaaattaaaa aatagtgcag tcgccgtggg
     4021 tgatgtcaat aatgacggat tacctgatgt gttcctgtgt ggtcaaaata ctcagggttt
     4081 tgcttcatca tcgatatatc tgaatgaagg cgctgccgga tttgccgaaa tgaatattgg
     4141 caatgcacct ttagaaggag taatctacgg aggaggcgct tttggtgatt cagacggaga
     4201 cggctttccg gaacttttgc tgtttggatg gaatttaaat aaaggagaac cacgtactta
     4261 cctatataaa aacaatggtg gccaaagttt tgcagagatg aatattgccc tgccggggat
     4321 tatttcaggt tttgcattat tcggagatat tgacggagac ggggatttgg atttgattat
     4381 cggaggcatt agcaagacat atgaatttat tacaaatatg tacaaaaatg acgggtcggg
     4441 aaattttagt cttgataatt ctacctcctt acctcaactg ggcaggtcac aggccgtgct
     4501 ggcagacctt aacaatgacg gacacctgga tctggtcatt acaggactag acaacaggta
     4561 taaccctgca gcatttgtat tgattaatga tggtaatggt ggtttggtcg taaaaccgca
     4621 gaccgaacta aagggcgtat accaggggtc agtatctgtt gctgatgtaa acggtgatgg
     4681 tttttctgat ttgtttctga caggctctac caaatatagc ggaagattat cagccttata
     4741 catcaatgac ggtaccggca gtttttctca atcaaatttg tccacattca acaacgtatg
     4801 gatcagttcg acacaatttg ccgatgtaaa caaagatggc cacatggatt taatcatgac
     4861 aggctctgat ttgaattatc aatctgtcat tcatctctac ctgaacaatg ggctcaatgc
     4921 cttttctgaa gtgcagcaaa ccaatctgga aggtgaaata tggggagatc tgattatgca
     4981 ggatttcgat aaagatggag atcccgatct tattattacc ggcgaaaatt acagatatga
     5041 aggaagtgcg gtgtattatt ccaatacaag cgaacttttg ccggaggcgc ccgtagtaaa
     5101 tcttggtgct gataccatcc tttgcagagg tcagcatgaa aatttcgtgt tggaagccgg
     5161 ttcagattct tcaggaaata agatattgtg gaacacgggc gatgtttctg acaagattgt
     5221 agtagatact tcaggcactt attcagtggt ggtaaccaat atctatggcc aaagtagcat
     5281 tgattcgata accgtgtggt ttaaagattc acccgaactg aatggcattg tgatatccaa
     5341 gctggactca cttacatttg attttgaagc cgaaggggca aatattgaag aattttactg
     5401 gaacttcgga gatgggagta tctcaactga atcaaaccct tcccacaagt atgaattgtc
     5461 gggagaatac atcgttctgc ttattgcttc caacccctgt ggccgtgaca cggtcatcca
     5521 aaatctcagt attattttat ctacgaatga tattagcaga cattcccggg tcaggatgta
     5581 tccaaatcct tcttctgggt acctgaatct tcattcggcg ggttcgccca tttcaacgat
     5641 caatgtaatc aatattgccg gcactttgtt gattaaccag cccgttgccc cttctgcaaa
     5701 atatacgttg gacttgtcag tacttgaggc agggaattat tttgtaagaa ttacattatc
     5761 cgatggttct gtattcaatc aaaaactgag catcatcagg taacagtaat tgttagagga
     5821 atagaatcat ggtattaaca gtgaccaagc tttctgcaat aaagttttaa actgcgctca
     5881 tcgaatcgaa tttacatttt aacccgcttc taatattccc ggcactccac agatagaatg
     5941 gtagcccagg gagcgaccgg taaattagtt ttgatacttc cgaaatctgc gaccggagcc
     6001 atggtctgcc ccactgtcgg ggcaattgga atttttccgg atgttgtcgt aataattact
     6061 ttgatgaaaa ttcaaggatg ataactttat gtatatttac aggaggttgt gtctgacaaa
     6121 tgaaacagta atagtgtcgt tcatcaagct atttgatgtt atggtgagac aaatgcttca
     6181 attgaacaat aatacaaaaa ttgaaagccc ggatctgcaa gtcaagccga catctcgtta
     6241 aacatcgctg attttgaact atacttctca ccttgtacca tcctggatca aaaaataggt
     6301 ttctttgtac tttagaatgg caagaggaaa aaaattgata tggctgcaaa ttattttggg
     6361 tgttgcattt ttgcttcagc ctatcctttt acctgtgaga cctgatgaca tgtatagatt
     6421 tgttttttca aaagaaatct taaaaaacat gttggccaat gtgctcattc ttgtattttt
     6481 ctattggaat tattactttc ttatccccgg attttacctg gcaaaaaaat atctgcccta
     6541 tttccttatt gtaattttat cattttcgac gattttgttt atttcaaact ctgtcacatc
     6601 ccaaatttcg cctgccaaac aaggcaaccg ggcatttcct gtaatagagt ttagaaataa
     6661 tcacccacag tttggattta gacaaattcc tacaccacag ggttcatttc tttcaaaatt
     6721 aaggttcttc tttaccgaaa acgatcaaat cttttttctt ttcggctcca tagttctttt
     6781 ttctttgtta ttacaaatga gcaatcgtta ttacaaaaca gaaaatgcca agcaagaagc
     6841 cgaaattaat tacctacttg ctcaaattaa tccacatttc ttgtttaaca cattaaacag
     6901 catttacacc cttgctatca tagaaaatgc caccaaggca tctaccggtt tgctaaaact
     6961 atccggttta atgcgttata ttattactga aacgcatcaa aatagtgtgc ctctggagaa
     7021 ggaacttgaa tgtatcaacg attttattga tttgcaaaaa ttacgcattg gcaaccatgt
     7081 aaaacttgat tacgacgtgt cagggaagat gtcagggaaa caaatagctc ctatgttgct
     7141 gataccattt attgaaaatg cttttaaata tggaataagc cccgatgaag attcagcaat
     7201 tcaaatcagt attcatgtga tagacaacgt attgacaatg aaggtatcta ataataaagt
     7261 cgcaatcaac aatgcttcta cagaaaactc aggtttaggt atagaaaatg ccaaaaatcg
     7321 attgaatcta ctatacccga acaaacacga tttaagcatt tcagaaaccg aaaaaaaatt
     7381 taatgtgact ttaacaataa cttgctgatg ttgcgtgcta ttgccataga tgatgaacca
     7441 ttaccactgg aattattgcg agcgttttgc tcccagacag atataatttc cttggagaaa
     7501 accttttcga aagttaatga agcacaactg tacctgcaaa atttcgaagt tgatctgtta
     7561 tttctggata ttcaaatgcc caaactatcc ggcattgagc tttacaaacg gctggagcag
     7621 gatactatgg taattttcac caccgcacac agtcaatatg caattgatgg atttaactta
     7681 aatgccgttg attttttatt gaaacccttt tcgtacgaac ggtttttggt tgcaataaat
     7741 aaagcaaatg attataaaaa ttataggcta aacaaggaat cttccgatat tcaacacttg
     7801 tatgtgcgtt cagattataa cttgaataaa atttctgttg cggacatttt attcatagaa
     7861 tcttttgcag attatctgga tatccacatt gcagacaata agaagataac cacacgcatg
     7921 tcactgaaaa gtataatgga aaaattacca caaaaggatt ttagacggat tcatcgttcg
     7981 tttattgttt ccattaaaca tattgaaaaa atgaatagaa gcagtataaa aattgcaaat
     8041 cgaaaaatcc ctattggcac gagctataaa gatgattatt acaagctttt ttagttttaa
     8101 ataaattttc ttcgcaggaa aacggctatt catcgcactt atttcatttc taatcaatga
     8161 aaatctcatc tttacatggc gcaatttaat ttttttaatg taatgaatat gaaagtaaat
     8221 ctaaaatttg tgaaaaacac ggtccttgca ggagctttgt ttttcttgtt tggtgcggga
     8281 tttgcaaccg cacaaacacc agtgaaagta actttactgg ataataccga acagaactat
     8341 actgtggaat ctaccgggaa aatttggttc agtggcagca atttggttat caatactgat
     8401 aatacattga ccccaactac aattcctttg tcaaatatcc gaaaaattac ttttgacggg
     8461 agtggcaata ctaacgcagt gaatgaagtg actaaaaaca aggaaactat cagtattttc
     8521 ccaaacccta ccaatgatta ttttgatata gaagcagagg gggcatcatc cttgagtgtt
     8581 tacatatata ataccaaggg ttcacaagtt gcaaagggta tatataaaag tggagaaagg
     8641 gtaaatgtat cgaatttaat accgggcatt tatgtcgtag ttatcaacaa tcaatccttt
     8701 aaacttatta aaatatgaaa aaaataagtc tgtttctttc tctaatactt ttggtattaa
     8761 gtatacagga aattactgct cagaatattt tgcgtgttca caatagtgat catcttattt
     8821 atcaacaaaa agttgaaaat atagatagtt taaaattagg gaacaacaat tctgattttt
     8881 ttccaaatac aggcatgttt gccttgccta tagccggtat tgacagcatt acatttgcca
     8941 ataatacgga tgatgaaatc tacattatct ataatggctc aagcgccaca atcatcaatc
     9001 cgttttcaac acaaggcgta agcattaagg atagtttagg acatgtaaca gcgacctcaa
     9061 cttatccaag ttccggtctt aaataccata ttttgggaac cacgaccaat ggttctttga
     9121 agataaccag tgcccaatct gttcgtttga ttatgagcca tgctgctgtt accaatcccg
     9181 gaggtgctgc tatttcaatc aatggagaca gtgaaatgta tttatcttcc ggcaccgtta
     9241 atacattgag cgatgcaaca actgcaagta ccagcggggc tttgtacgta aaaggcaact
     9301 tgagtatttc cggatcgggc actttaaacg taaacggata caaaaaacat gcgattttag
     9361 ttgatgcaac attagttatt gaatcgggca tcattaatat tgcagaggct gcatcagacg
     9421 gtattcatgt aaatgattat acacaaaatg gcggaacaat tactattatt ccaaccggtg
     9481 atggtatcga tgtgtctaac gcccttattt tgaatgatgg tgaattaacc gttgaagctg
     9541 cttccgatga tgtaaaagga ttaaaggcaa ccactgttac catcaatgat ggaacaagta
     9601 acattacggt ttccggagca caatcaaaag ctataaaatc gagtgggacc actaccataa
     9661 atggcggcac aacctctgtt acggcttcag gttctgttgt attagaagct tcaggtagtg
     9721 gttttgaccc ttcctattgt acaggaataa aaagtgatgt ggacgtgata attaataatg
     9781 gtactttaac cattcaatgc ctggcttcca attcaggcgg aaaggggatt tcaacagatg
     9841 gcaacataac tataaacgga ggaagtgtaa atgtctcaac agcaggtaat ggtgcgactt
     9901 atactgacga aaatggagtg atcgacagtt atacctctac aggacttaaa gcggatggga
     9961 atattttgct attagccgga aatattactt gttccagttc gggaacagga ggaaaaggtg
    10021 tatcagcaga cggaaccatc accattggaa atccaggtgc cgttgatgac ttactggttt
    10081 tgaatgttac cacttccgga aacaggtttt atgtttcagg ttcaggacag aatgctgatt
    10141 atgccaaccc taaagcaata aaagccgaag gtgatttgac ggttaatagt ggcctcatta
    10201 ctgtaaattg tacacaaaat acggaaggtg gtgaaggctt ggagagtaag gctaatttat
    10261 atatcaaagg aggtcaaata actgcaacaa cctatgacga ctgtgtcaat gctgctaccc
    10321 atattgaagt atcaggaggt actcattctt taactgccag aggcaatgac ggtatggatt
    10381 caaatggaac tcttactatt tcgggcggtt taatcgtctc aaaaggagcc ggtgggccgg
    10441 aagaaggttt tgattgtgat aacaacacat ttaaagtttt aggcggtatt atggtaggaa
    10501 cagggggtaa tacaagcaac cctacgacag gtgtttcgac ccaaaattca ctaaaactat
    10561 ctattacgcc caatcaaaac atctgtatta aaaatgcagc aaatcaaacc atattgattt
    10621 atgccttacc ggctcttaca ggaggtggtg gcccgggtgg aaacaataaa atggtcatgc
    10681 tgtttagcga ccctgctttt gtaaatggca catataccat tcaatatggt ggaacaataa
    10741 gtggtggaac aaactttaat ggttactaca ccggtgcgac ctattcgggc ggttccagtc
    10801 agacatttac agtcagtgca aagtatacaa cagtaaatgt tccttaactt tttaaagaat
    10861 agttaaatat ggaaaaacat cacaaataat tctttcaaat aaaagaatta tcataatcga
    10921 cttcattaat tccggttgat atttgctgta aaaaccgaca ataaaaatga aggatatttt
    10981 taataacagc cttgaaactt agtaaatttt caaggctgtt ttaattttcc acaatcaatt
    11041 actccacaaa taaaattgcc ccgttaatat ttttcagatc tttcgactat agcaccaagg
    11101 caagttgcat attgtgtagc aaactactaa taccgaaggt acattcgtta tagcttcata
    11161 agcatggcga tgctaaggta tacgcttccc gggtggcccc ctttgctccg taatattcac
    11221 tctaatgaac atccgttgtt ccaatactca tatagtataa aaacatgact gcccaacaga
    11281 cgataagatt tgccggattt ttgcagctga tgattgaaca acaatggcac ggtgtgtact
    11341 taccgaaaaa agcattttac tgaataaata ccgattcacg tcgaaagaaa tacgcccttt
    11401 gtccataaag gggaatcttt tacacgcctg aagtgttttt tgaaaaaatc tattgacatt
    11461 taatttaata aaaatgaaac gttctgttgt tattttgatt gtagttgcag tgattgcgct
    11521 gattgcttat tcttctttca agggaggtta taataatatg gtgaccaagg aagaagcggt
    11581 tgaagccgcc tggtcccagg tagaaaatgt gtatcagcgc cgtgccgacc tgatacccaa
    11641 ccttgtcaat gttgtgaagg gggctgccaa ttttgagaaa gagactctga caggagttgt
    11701 agaagcccgc gctaaggcta cacagatcac ggttgatccc aaaaacctga cacctgaaaa
    11761 gattcaggaa ttccaagcgg cccagggaga attatctcag gctttgggca aactattgat
    11821 gatcaccgaa aactatccgg aattgaaagc taatcagaac ttcagggatt tgcaggtcca
    11881 gctggaaggt actgagaaca ggattacgca agagagacgt aaattcaacg aggtggtgca
    11941 ggatttcaat acttatatca gacaattccc gaggaatctc atagccggca tgtttggttt
    12001 tgagaagaaa gggtatttcg aggcggataa aggcgctgaa aaggcaccga ccgtggattt
    12061 caactgatac agactcgtct gactaacacg actcactaac atgtccagac tatttaatcc
    12121 cgaacaggag aaacggattg ttgaaggtat aacaaagttg gagagcaact ccaccggaga
    12181 gctcaggatt ttcgtagagg atagctgtaa tggaaacctt cacgaccgcg ccatagaggt
    12241 ctttgttgag catggtatgc agcatacctt aaacaggaat gcggtgctga tatacatagc
    12301 ggtgcggtcc aggaagattt atgtctgggg cgatgaaggg atccatgcca aagccggaca
    12361 gggtctttgg gatcaaattt tggcggatct gacagctgac tttaaggaca gcatgtttga
    12421 ggcaggcatc ttaagggcca ttgaggcgat cggcagtaaa cttgccgagt tttttccccg
    12481 ggatggcacg gagggtaata atgaattgcc ggacgatatt atctacggat tttgaaccat
    12541 atcagtaatg atgtctagga tgat
//
LOCUS       DAOQQN010000034        12513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1753_length_12513_cov_5.622251, whole genome shotgun sequence.
ACCESSION   DAOQQN010000034 DAOQQN010000000
VERSION     DAOQQN010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12513
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1753_length_12513_cov_5.622251"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(657..2051)
                     /locus_tag="PLG24_02265"
     CDS             complement(657..2051)
                     /locus_tag="PLG24_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HMZ23737.1"
                     /translation="MNHTYHDLVNQTFYFPQEGFGLEDNKLLFHGIKLYELIEKYGTP
                     LKLVYLPKISDQIKKAKNFFDTAIFQLQYTGKYNYTYCTKSCHFRYVLEEVLKNDVHL
                     ETSSAFDIDLIWKLYADGLLHKDKIIVNNGFKNDAYIRRICGMIQSDFTNVLPVMDNW
                     EELDKFSQYLGDREFNVGIRVATEEEPHFEFYTSRLGIRKSEILNFYHEKIAQRPNIS
                     LKMLHFFVDTGIKDNVYYWDELRKGIKAYCELKKACPTLEAINIGGGLPIKNSLGFEY
                     DYGYMILEIVRQIQDACAENGIPEPDIYTEFGKYTVGESGAHFFSVIGQKQQNDNECW
                     YMVDNSLMNTLPDAWGINEHFILLPINHWDHEYKRVNIGGLSCDNSDYYNSEAHSNQV
                     YLPAVGKEEKLYLGFFHSGAYQDALSGYGGIKHCLLPSPKHVLIDRNPEGELVDWVWA
                     NDQDSESMLKILGY"
     gene            complement(2087..3484)
                     /gene="lpdA"
                     /locus_tag="PLG24_02270"
     CDS             complement(2087..3484)
                     /gene="lpdA"
                     /locus_tag="PLG24_02270"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HMZ23738.1"
                     /translation="MKYDILVLGSGPGGYVAAIRASQLGFKTAVIERESLGGICLNWG
                     CIPTKALIKSAKVFEYLNHAGDYGITVDGGKADFGAIVKRSRGVAEGMSKGIQFLLKK
                     NKVDTIYGNGVLKKGKKIEVAAADGSKTTYEANHIIIATGGRAKELPGIAIDHKNIIG
                     YREALALESLPESMVVIGAGAIGVEFAYFYHTLGTKITVVEFMENGLVPREDAEISKE
                     LGKIYKKKGMNILGNTAVEKVDISGSKKKVTVKDRKTGATQVIECDIVLSATGVTPNV
                     ENIGLEEVGIQLDRGHIKVDSFYKTNVDGYYAIGDVIPTQALAHVASAEGITCIENIA
                     GMNPHSIDYNNIPGCTYCSPEVASVGYTEAAAKEAGYELKVGKFPFSASGKAAASGAK
                     EGFIKLIFDAKYGELLGAHFIGENVTEMIAEIVVARKLETTGHEMIKAIHPHPTMSEA
                     IMEAAAAAYGEVIHI"
     gene            complement(4130..4882)
                     /locus_tag="PLG24_02275"
     CDS             complement(4130..4882)
                     /locus_tag="PLG24_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23739.1"
                     /translation="MITYFTSFIYGWIGLAIFIFFILLFITAPYGRHTTKTWGPLIDN
                     RSAWIIMEIFVLIVINYFVFTGRLVQTPVNYIILGLFSLHYLNRSLIFPFRLRTSGKK
                     MPVIVMLMGMTFNLANGFIIGYFLGNIKTYELEWLTTPQFIAGIIIFFTGMIVNWRAD
                     GILIRLRAPGEIGYKIPKGDMFRFVSCPNLLGEIIEWGGFCILTWSLPGLAFFIWTFA
                     NLVPRAISHHKWYKQRFNDYPPERRAVIPFVI"
     gene            complement(4892..5869)
                     /locus_tag="PLG24_02280"
     CDS             complement(4892..5869)
                     /locus_tag="PLG24_02280"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMZ23740.1"
                     /translation="MKVAVTGASGHIGASLCRELLHHGYEVVALIHRDISALKGLPIS
                     MVQGDILNTESIKQLLGQCDMAIHAAAAIELAYKFDKNVYDTNVLGTINVLETAQEMG
                     VRRLVHFSSIHAFSQACYDQPLDETRAFVNDNSIFYDQTKRDSHRLALNACEAGLDVV
                     ILNPTSAIGPPDQKPSLLGKAVIDLYKGSLPFVVQGGFDFCDVRDIAFGAVKALEKGR
                     KGEAYLLSGHYHSIKELADFVMEAKNSKKRLVELPLYIANIAFPFVKFYSWLTKKPPL
                     FDKTYIDILQDGNKNVSSAKAEAELGFTSRDLRESVEDTVKWFKDNGRL"
     gene            6342..9116
                     /gene="acnA"
                     /locus_tag="PLG24_02285"
     CDS             6342..9116
                     /gene="acnA"
                     /locus_tag="PLG24_02285"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HMZ23741.1"
                     /translation="MSYSKAKSVKELSVNGKSYHYSSLKDLDVNIDHLPFSIRILLEN
                     VLRNYDGFSITDEHIDTLLNWKPATEDKDIPFKPGRILMQDFTGVPAVVDIASLRAEF
                     VRHGKDGQKINPAIPVDLVIDHSVQVDYFGTDYAYDKNVEIEFERNKERYELLKWAQK
                     GMSNFTVVPPGMGICHQVNLEYLAKGVTARDGWLFPDTLVGTDSHTPMVNGIGVIAWG
                     VGGIEAEAAMLGQPIFFTLPEVVGLKLTGKIPDHCTATDMVLTITRLLRDAGVVGKFV
                     EVFGDGLNNLTVTDRATISNMSPEFGCTVTYFPVDNRTLEYMHSTNRSEEQIRIVEAY
                     CKENMLWREGEDKIKYSSIVELDLTTLEPTVSGPKRPQDKIFVKDLSGKFCEILKKEF
                     NRNYHPQSERAEYAMLIEGGSGTEFTRGKVPMNGESSYEVVADQDHRSVRIKQPNKEF
                     VLSDGSIVIAAITSCTNTSNPAVMVGAGLLARNAVERGLRTKSWVKTSLAPGSKVVTK
                     YLERSGLNVDLDALRFHTVGYGCTSCIGNSGPLPSAIAEAVDKGELVVASVLSGNRNF
                     EARVHPQVKMNFLMSPMLVVAYALAGRVDIDLINEPIDMDPNNQPVYLRDIWPTRDQI
                     QLTINECMKQGDFEEVYDIIFDGSTDWQSLEVNLTQNFEWNKDSTYIREAPFFENISA
                     TPDEIKDIEGARVLLYLGDSVTTDHISPAGSFKEDSAAGKYLIDNGIERNEFNSYGSR
                     RGNHEVMMRGTFANVRIKNKVADKEGGFSRYMPTGEVMTVFDTAMKYQNDNTPLIILA
                     GKEYGSGSSRDWAAKGTFLLGVKAVIAESYERIHRSNLVGMGVAPLVFVDGQNAQSLG
                     LDGTEVFDIEGLADNLTPHKILNVKAVHSSGKVTEFKVEARLDAPIEIEYYKNQGILQ
                     YVLRDYLKNN"
     gene            9218..10207
                     /locus_tag="PLG24_02290"
     CDS             9218..10207
                     /locus_tag="PLG24_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23742.1"
                     /translation="MVGDIYDPFESLDFSEETCFLTGEKLDGRNNFTVSAFPEWVMDK
                     YKLNEAYLLMLSGTRLQYKELRMPASKHAFQVVKALDENTQAAFQEGFEAVKHIPDLL
                     LFQWMARVFYGVLYGDCKQGIIEAKEKGQEFRMAPQLKRKFKNLHLMLQSLIRPVEFR
                     NFTPWTICRYQVNISKDIFNYKDEIQHLNFSFGMKDFGIAACLQDNGAVASYHRNLLQ
                     KVEGQPLHPAQFEELYGHLVYANYLMKDFEDYQISLEDDRIVLELAENEATGSIKFAK
                     WEDEIFAQVLTNMWQPWGIPLELVYRFPNSPLSYLINEQTNEFIPKERVELDY"
     gene            10227..11336
                     /gene="corA"
                     /locus_tag="PLG24_02295"
     CDS             10227..11336
                     /gene="corA"
                     /locus_tag="PLG24_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HMZ23743.1"
                     /translation="MNRKLFKKSKAVKNIFRQDANRSASIKTGLAPGTLIFTGIQKVE
                     HPFIRVVLFNENNIEQYVISDGKVGNIRSGQLKTWIDCVGLHDVGLIEALGKKFNISR
                     MVLEDILNIGQMPKYINLDKGNFLTIQSFVQNRGTEELSKEQLSVYFEDNLAITFRED
                     ERNDFNPIYSRIVNPESRLVKNDIYYLVYSMLDLTVDNYLETAHYFESEIEKYEILIE
                     TRPDNDLKKEIFNFRRSFLYFFKMIAPLEEAISRFKADDEDLVNSVTKIYISDLLDHC
                     RRLRNLAETYNEMIYMLYDLLHTEINYRSNTIITTLTVISAIFIPLTFIVGVYGMNFE
                     YMPELTWNYGYYLIWAIMILFTISAIVYFRVKKWI"
     gene            complement(11370..12227)
                     /locus_tag="PLG24_02300"
     CDS             complement(11370..12227)
                     /locus_tag="PLG24_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23744.1"
                     /translation="MKYSGLTYLIAFVLAIAIVIYQYSVYGTWLSPYLLIPVILGVTG
                     YTLGPQIDWWFYKRFPPKLDDKVKRIFEINSEFYRNLPPEKKELFDTRVCLYIEDKDW
                     QYVSMDEEMPYDIRAIIAFEPVRLTINLENFLMRPFDRIAVYQNPFLSPFYPDRYHGS
                     ELYSEDGIVLFSLKHIIPAFRQPLEYFNVVLYEYARIMIATYKNLDFPKLDDHFWYDL
                     YKVANYGHREIMGAIGLPDVDALSVAIHHYFVYGQYFRTIYPELCIQLDMIFESDKNF
                     GQLALTNNL"
     gene            complement(12320..>12513)
                     /locus_tag="PLG24_02305"
     CDS             complement(12320..>12513)
                     /locus_tag="PLG24_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23745.1"
                     /translation="QGHKDFQSFALPTELRYQSGCKYRTYFQFDNIFDKKIFFQCLHS
                     ILKVIIVADPFLKEDPLRP"
BASE COUNT         3753 a         2332 c         2616 g         3812 t
ORIGIN      
        1 aggtccgggg gttcgaatcc ctctctctcc gcagggaaag cgttgtaagt tgattcttgc
       61 aacgcttttt tattttatgg cataggccag cagcactgca ataactaata acgctaagcc
      121 aggtccaggg gctggtgtgg agaaaattat ttttgcaggg tcaaattcca agtaagtaga
      181 tgattaatag tctcaaatta ttgctatgat ggagttttta gctgacaaga aaaataaaat
      241 tgcagatatc aactaaatat tgcatacaag gtctctgaaa acaaggctag ctatgattta
      301 gtcgttatta aacatctacc tacttcccga gaatccacct ccttcctacg tatccaattg
      361 ctgccgattg aaaaattcaa agatgcagat gtttaatagt catcataaat ttatgccatc
      421 tgctgacttt cgatcttgat taatttttta cgattgcatt cagatagttt ttatcctcaa
      481 ggtaccatta aggatattct ggagttgcat gggaatttat gaaaatctat aggtgaattc
      541 attgtcccaa gacgcaattc cgtgaatgtt aaccagcttg catacctcaa gtacctggca
      601 gctgaaaaaa aagaagttaa atcaaataac aatcttcggc gtatgctttt atgatcttag
      661 taacctaata tcttcagcat agactctgaa tcctgatcat tggcccatac ccaatctact
      721 aactcgcctt cagggttacg gtctatcaat acgtgctttg gtgaaggcag aaggcaatgc
      781 ttgatacctc cataaccact caaggcatcc tgataggctc cgctgtgaaa gaatcccaga
      841 tatagttttt cctctttacc tactgcagga agatatacct gattactgtg agcttcggaa
      901 ttgtaataat cggaattgtc gcaactaagt ccaccgatgt tgactcgttt gtactcatgg
      961 tcccagtgat tgatgggtaa caggatgaag tgttcattga ttccccaggc atccggtaag
     1021 gtgttcataa gggagttatc gaccatatac cagcattcgt tgtcgttttg ttgtttctgg
     1081 cctatgactg agaagaaatg tgctccgctc tcaccaacgg tgtatttgcc aaattccgta
     1141 tatatatcag gttcagggat gccattttct gcacatgcat cctgaatctg acgcacaatc
     1201 tccaggatca tgtatccgta atcgtattca aaacccaaac tgttcttgat aggcagaccg
     1261 cctccgatat tgatcgcttc aagggtgggg caggccttct tcaattcgca atatgccttt
     1321 atacccttgc gaagctcgtc ccaataatac acgttgtctt tgatgcctgt atctacgaag
     1381 aaatgtagca tcttcaggct gatgtttgga cgttgggcga ttttttcatg gtagaaatta
     1441 agaatctctg attttcggat gccaagcctg gaggtataaa attcaaaatg tggttcttcc
     1501 tccgtcgcaa cacggattcc tacattaaac tcccggtctc cgaggtattg gctgaattta
     1561 tccaattctt cccagttgtc cataacagga aggacgtttg tgaagtcgga ttgaatcatt
     1621 ccgcagattc tacggatgta tgcatcattc ttaaatccgt tatttacgat gatcttgtcc
     1681 ttatgcagca gcccatctgc gtatagcttc catattaagt caatatcaaa tgctgaagag
     1741 gtctcgagat gtacatcatt cttcagtact tcttcaagaa catagcggaa gtggcagctt
     1801 ttagtacaat aggtataatt gtatttgccg gtatactgca attgaaaaat ggcagtatcg
     1861 aaaaagtttt tggctttctt gatctgatca gatattttgg gcaggtacac gagtttaagt
     1921 ggtgtaccat atttttctat cagttcatat agcttaattc catggaaaag gagtttattg
     1981 tcttcaagac cgaatccctc ctgtggaaaa taaaaggtct gattgaccag gtcgtggtaa
     2041 gtgtggttca tccccccagc ccttgtattc tttttttatg aataaattaa atatgtatta
     2101 cttctccgta tgcagcagca gcggcctcca tgatggcttc gctcattgtc ggatgtggat
     2161 ggattgcctt gatcatttca tgtcctgttg tttccaactt acgcgcgacg acgatttcgg
     2221 caatcatctc agtgacattt tcacctatga aatgggcacc aagtaattcg ccgtattttg
     2281 catcgaaaat caacttgatg aatccttctt tcgcaccgga ggcagctgct ttcccggatg
     2341 ctgaaaatgg aaatttgccc acttttaatt cataaccggc ttcttttgcg gcggcttcgg
     2401 tatatccaac agatgccact tcaggtgaac agtatgtaca acccggaata ttgttgtagt
     2461 caattgagtg tggattcata ccggcaatgt tttctataca agtgatgccc tcagcgcttg
     2521 ctacatgtgc cagggcttgt gtcgggatga catcacctat tgcataataa ccgtccacat
     2581 tggtcttgta aaaggaatcc accttgatat gtcctctgtc caattggatg ccgacttctt
     2641 caagacctat gttctctaca ttgggcgtta ctcctgtcgc tgaaagcact atatcgcatt
     2701 cgataacctg tgtggcgccc gtctttcggt ccttgacggt taccttcttt ttagaaccgg
     2761 aaatatcgac tttttcgaca gctgtgttac ccaggatgtt catgcctttt ttcttgtata
     2821 ttttgccgag ttccttcgat atttctgcat cttcccgagg gacaagaccg ttttccataa
     2881 attctacaac agtaatcttc gtgcccagcg tgtgataaaa ataggcaaat tctacaccga
     2941 ttgcacctgc gccgatgaca accatggatt cggggaggga ttcaagagca agtgcttcgc
     3001 ggtatccgat gatattttta tggtctatgg ctatacccgg caattctttt gcacggccac
     3061 cggttgcaat gatgatatga ttggcctcat atgttgtctt tgaaccgtct gcggcagcca
     3121 cttcgatttt tttacctttt ttcaggacgc cattgccgta tattgtatcg actttatttt
     3181 ttttcaaaag gaattgaatg ccttttgaca tgccctctgc aacgccacgg cttcttttga
     3241 cgatagcccc aaaatctgcc tttcctccat caactgtaat accgtagtca ccagcgtggt
     3301 tgagatattc gaataccttg gcacttttta tcaacgcttt ggtagggata catccccaat
     3361 tgaggcagat gccacctaag gattcccttt caatgactgc tgttttaaat ccgagttgag
     3421 aagctctgat tgctgctaca taacctccgg gaccacttcc caagaccaaa atatcgtatt
     3481 tcatgctgaa tttcgtttag tattttagct aattaagtcg tttttcaaaa aatcgtgcaa
     3541 atatatgtaa ttgtgaggac ttcctcaggg acattttcta aattgatact attggaacaa
     3601 tacgttaatc cgtgaagtca ggacttaaat aggttccttt gcctgggtta tacccggata
     3661 catgcaccgt aatgaggcta tagatttctt tttcttgtat ccttaccggc aagttgactg
     3721 gtatattttc aatttatcca taaatagata cccgggaagc aattacattt ccatccaacg
     3781 acttgaatat ttctttgaaa tattattgat atcattggct tcctgttgca ttgatgtggt
     3841 tgtattaact gactgtcggg cataattgag agctattagt ttgaatgaat ggagtttggt
     3901 aattcaattc atgtttttca atgttaacaa aacaggggca ctaataagaa ttttttacga
     3961 gtcgtccgcg tccaatatac taaacaaaaa atgaaaatgg aataatataa atgcaacaag
     4021 tacgaaatca aagatgagtt atcgatcagc tcctacaaat aaggggtatc gtgtcactat
     4081 tattatcaat agcatattat tctaatgtct ataactctgc tctgtacaat taaatgacaa
     4141 atggaatcac agcccttctt tccggtgggt aatcattaaa tcgttgcttg taccatttgt
     4201 gatgggatat ggctctcggc acgagattgg caaaagtcca aatgaaaaaa gcaagccccg
     4261 gaagactcca ggtgagaatg caaaatcctc cccattctat aatttcacct aacaaattag
     4321 ggcaactaac aaatctgaac atgtcacctt taggaatctt atatccaatt tcgcccggag
     4381 ctctcagacg aattaaaatc ccatcggctc tccaattgac gatcattcct gtaaagaata
     4441 tgatgatccc tgcaataaat tgtggtgtag ttaaccattc taattcataa gtcttgatgt
     4501 ttccaaggaa ataacctatt ataaagccat tggcaagatt gaaggtcatg cccatgagca
     4561 tgactataac aggcatcttt tttccggaag ttctgagtct gaatggaaat atgaggcttc
     4621 tattgaggta gtgtaaggaa aacaatccca gtatgatata attgacaggt gtttgaacca
     4681 atctgcctgt aaaaacaaaa tagttgataa ctattaaaac gaaaatctcc atgatgatcc
     4741 atgctgaccg attgtcgatc aaaggacccc aggtttttgt cgtgtgtctt ccgtaagggg
     4801 ctgtaataaa taaaagtata aaaaaaatga agattgccaa ccctatccat ccataaataa
     4861 atgaagtaaa atatgttatc atagaattag attagagtcg accattatct ttaaaccatt
     4921 tgaccgtatc ttcaaccgac tctcttagat cccttgatgt gaagcccaat tccgcttctg
     4981 cttttgctga gctcacattt ttattaccat cctgaaggat gtcgatatag gttttatcaa
     5041 ataaaggtgg tttttttgta agccatgaat aaaacttaac gaaaggaaaa gcaatattgg
     5101 caatatataa aggtaattca accaatcttt tttttgagtt ttttgcctcc atgacaaaat
     5161 ccgccaattc cttgattgag tgataatgtc cgctaagaag atacgcttca cctttacggc
     5221 ccttttccag ggctttcaca gctccgaaag ctatatcccg cacatcacaa aagtcaaatc
     5281 caccttgaac cacgaaaggc agagatcctt tgtacaaatc aataactgct ttgcctaaca
     5341 atgatggttt ttggtcaggt gggccaattg cagaagttgg attcaggatg acaacatcaa
     5401 gtccggcttc acaggcattt aaagccagcc tatggctatc tcgtttggtt tgatcgtaaa
     5461 aaatcgaatt gtcattaaca aatgcccttg tttcatcgag tggctgatca tagcaagctt
     5521 gagaaaaggc gtgaatagaa ctgaaatgga ccaaccgtct aactcccatc tcttgggctg
     5581 tttctagaac attgattgta cccagtacat ttgtgtcgta tacatttttg tcaaacttgt
     5641 aggctaattc gatggcagca gcggcatgaa ttgccatatc acactgtcct agaagttgtt
     5701 ttattgattc agtattgaga atatcgccct gaaccattga aattggaagt ccctttaaag
     5761 cagaaatatc tctatgtatc aatgcaacta cttcataacc atgatgtaat aattcccgac
     5821 agagggaagc gccgatatgg ccggaagctc ctgttacagc tactttcatc agccgaagtt
     5881 ataatttttt gaaaattttt tgaaattaat gatgaagaat tattaaatta tatatatgta
     5941 ctatatttat ttttataaac ataacaaaac caatacaaaa tactttaatt atatatatta
     6001 tgtattaaat atgaactata ttcattttga ataaaatgga gaaagggtaa agcgatgtag
     6061 ctaatgaata attttcatgt attagttatt ctgatcttgc attattttgt tttttgtcat
     6121 tcattaacag tataatacct tgaagttatt atcattaggt attgatttat ggttttgggg
     6181 ctggtaaatt cttgttatta acaacctttc ttctgatttt tcattatgat ataacattat
     6241 atccattaag tgtaattttg aaattttcgg cttaccaact ccgggagttt ttatagattt
     6301 acgttttttt agcaattcat taaatttcaa taaatagaga tatgagttat tcgaaagcaa
     6361 aatcagttaa agagcttagt gtcaacggaa aatcatacca ttattcctct ttgaaggatt
     6421 tagatgtcaa tatcgaccat cttcctttca gtataaggat tcttttggaa aatgtactta
     6481 gaaactatga cggtttcagt ataaccgacg agcacataga tactctgctc aactggaagc
     6541 cggcgactga ggacaaagat attccattca aaccgggacg tattttgatg caagacttta
     6601 caggtgtacc tgcagttgtg gacatcgctt cacttagggc tgagtttgtc cggcatggta
     6661 aagatggaca gaagatcaat cctgcgatcc cggttgacct ggttatcgat cattcagttc
     6721 aggtcgatta tttcgggact gattatgcct atgacaaaaa cgttgaaata gagtttgaaa
     6781 gaaataagga gcgttatgag ttgttgaagt gggctcaaaa gggtatgagc aattttaccg
     6841 ttgttcctcc cggtatgggc atctgccacc aggtcaatct ggaatatctt gccaaagggg
     6901 tcactgcacg tgacggatgg ttgtttccgg atacattggt tggcactgac tcacatacac
     6961 cgatggtcaa cggtatcggt gtcatagcct ggggtgttgg aggaattgaa gcggaggcag
     7021 ccatgttggg acaaccaatc ttttttactt tgcctgaagt agttggcctg aaactgacag
     7081 ggaaaattcc tgatcattgt acagctactg acatggtgct cacgattacg agattgcttc
     7141 gtgacgccgg agtagtaggg aaatttgtag aagtattcgg cgatggactt aataacctga
     7201 ctgtgactga cagggcaaca atttccaata tgtctccgga attcggatgt accgtaacct
     7261 attttcctgt cgacaacagg acactggaat atatgcattc caccaatcgg tctgaagagc
     7321 aaatcaggat agttgaagcc tattgcaagg aaaatatgtt gtggcgtgaa ggagaagata
     7381 agattaagta ttcttcgatt gtagagcttg atcttacaac actcgagcct actgtttcgg
     7441 gtccaaaaag gcctcaggat aaaatatttg ttaaggatct ctcaggaaag ttttgtgaaa
     7501 ttctaaaaaa ggaattcaat agaaattatc atccacagag cgaaagagct gaatatgcaa
     7561 tgctgattga aggggggtca ggaactgaat ttacgagagg aaaggtaccg atgaatggcg
     7621 agtccagcta tgaggtggtt gctgatcagg atcatcgttc agttaggatc aaacagccca
     7681 acaaggaatt tgtgttaagt gacggaagca ttgtcattgc agctattacc agttgcacaa
     7741 acacatcaaa tcctgcagtg atggtcggag ctggtctcct tgccagaaat gcagttgaaa
     7801 gaggccttcg gaccaaatca tgggtaaaaa cgtcacttgc tcccggttca aaggtcgtaa
     7861 ccaaatattt agaaagatca gggcttaatg tagacctgga tgcgcttcgt tttcatacgg
     7921 ttggttatgg atgtacatct tgtatcggga attccggccc acttccttcc gctattgcag
     7981 aggctgtaga taaaggggaa cttgttgtgg catctgttct ttccggaaat agaaattttg
     8041 aagctagggt acatccacaa gtcaagatga atttcttgat gtcacctatg cttgtagtag
     8101 cttatgccct tgcagggcgg gtcgatatag atttgataaa tgagccgatt gacatggacc
     8161 ctaacaatca gcctgtatat ctcagagata tttggcccac acgagaccaa atccagctta
     8221 ccatcaatga atgtatgaag cagggtgatt ttgaagaggt atatgatatt attttcgatg
     8281 gttctactga ctggcaaagt cttgaggtga atcttactca aaactttgag tggaataaag
     8341 actctaccta tatcagagaa gctcctttct ttgaaaatat tagtgcgacg ccggatgaga
     8401 tcaaagatat cgaaggggcc agggttttgt tatatttggg agactctgtc acaactgatc
     8461 atatttctcc ggcaggttca tttaaggaag actcggctgc cggtaagtat ctgatcgata
     8521 atggcattga gcgaaatgaa ttcaactctt acggttccag aagaggaaat catgaggtga
     8581 tgatgagagg cacatttgcc aatgtacgga tcaagaataa ggttgctgac aaggaaggtg
     8641 gatttagtag gtatatgcca acaggtgaag taatgacagt atttgatact gctatgaaat
     8701 accagaatga caatacacca ttgatcatcc tggctggaaa ggagtatgga tccggttctt
     8761 ctcgtgactg ggctgccaag ggaacctttc ttttaggcgt taaggcagtt attgcagaaa
     8821 gctacgagcg tatacacaga agtaatctag ttggtatggg cgtcgctcct ttggtatttg
     8881 tggatgggca aaatgcgcaa tcactaggtt tagatggtac ggaagtattt gatattgaag
     8941 gcttagccga taatctgaca ccacataaga tacttaatgt aaaggcggta cattcttccg
     9001 gtaaagtaac tgaatttaag gttgaagcca ggctcgatgc tcctattgaa attgaatact
     9061 ataagaatca agggattctt caatatgtat tgagagatta tttaaaaaat aattagaata
     9121 gaacctattg cgacaaaagc ctctgtactc agaggctttt gtattaatag agatcgcttt
     9181 caaaaaaatg aatttactaa attgtctgat aggctaaatg gtaggggata tatacgatcc
     9241 atttgaatcg cttgatttct cagaagaaac ttgcttcctg accggtgaga aacttgatgg
     9301 aaggaataac ttcacggtgt cagcattccc ggagtgggta atggacaagt ataaattgaa
     9361 tgaagcttat cttctgatgc tgagcggaac aagattgcag tataaggaac tgaggatgcc
     9421 agcctcaaaa catgcctttc aggtggtgaa ggcactcgat gaaaacacac aagcggcgtt
     9481 ccaggaagga tttgaagcag taaagcatat accggacttg ctgttatttc aatggatggc
     9541 acgtgttttc tatggcgtat tgtatgggga ttgtaaacag ggaatcatcg aggcaaagga
     9601 gaaagggcag gaatttcgaa tggcaccaca attgaaacgg aaattcaaga atctgcattt
     9661 gatgctgcaa tccctaatcc ggccggtaga attccgtaat tttactccat ggacaatctg
     9721 tcgatatcag gtaaatattt cgaaggatat cttcaactat aaggacgaga ttcagcatct
     9781 taacttcagt tttgggatga aagattttgg catcgccgcc tgccttcagg acaacggggc
     9841 tgtggcaagc tatcatcgaa atctattgca aaaggtggag gggcaacctc ttcatccagc
     9901 ccagtttgaa gaattatatg gtcatctggt ttatgccaat tatctgatga aggattttga
     9961 agattaccaa atcagtttag aggacgatag gattgttttg gaattagctg aaaacgaagc
    10021 aaccggaagc attaaatttg ccaaatggga ggatgaaata ttcgctcagg tattgaccaa
    10081 tatgtggcaa ccttggggca ttcctttgga attagtatat cgatttccaa attctccttt
    10141 gagttatctg atcaatgagc aaaccaatga atttattccc aaagagaggg tggaattgga
    10201 ttattgagaa ttgataaatt tgtctcatga atagaaaact ttttaagaaa tcgaaagcag
    10261 taaaaaatat ctttcggcag gatgccaacc gatccgcgtc aatcaaaacc ggtttggccc
    10321 ccggtacttt aatttttacc ggaatccaga aggttgagca cccattcatt cgggttgtct
    10381 tgttcaatga aaataatatc gagcagtatg ttatctctga tggcaaggtt ggaaatatac
    10441 gtagcggtca actaaagacc tggatcgatt gtgttgggtt gcatgatgtt gggcttatag
    10501 aggcattggg gaaaaagttc aatattagca ggatggtttt agaagatatc ctcaatatag
    10561 ggcaaatgcc aaaatatatc aatcttgata aagggaattt cctaaccata caatcatttg
    10621 ttcaaaatag aggtacagag gaactatcaa aggagcaatt atcggtttat tttgaagaca
    10681 acttagcgat cactttcagg gaagatgaaa gaaatgattt taatccgata tattcaagaa
    10741 ttgttaaccc ggaaagcagg cttgtgaaga atgatattta ttatttggta tatagcatgt
    10801 tagatcttac ggtggataat tatcttgaaa cagctcatta ttttgagagt gaaattgaaa
    10861 agtatgaaat acttattgaa acaagacccg acaatgattt gaaaaaagaa atattcaatt
    10921 tcaggaggtc atttttatac ttttttaaga tgattgcacc tttggaggag gcaataagcc
    10981 gatttaaggc ggatgacgag gatttggtca attctgtgac aaaaatatac atatcggact
    11041 tgcttgatca ttgcaggcgg ttgagaaacc ttgctgaaac ctacaatgag atgatataca
    11101 tgctatatga tttgttgcat acggaaatca attatcggag caataccata ataacaacat
    11161 tgacagttat atcggcgatt ttcatccctt tgacctttat tgttggggta tatgggatga
    11221 attttgaata tatgcctgag ctgacatgga attatggata ttatttgata tgggcaataa
    11281 tgatactttt tacaattagc gccattgttt atttccgtgt aaaaaaatgg atctaacagg
    11341 tagacatttt atgcgcaatt aattgagggt tacaaattgt tagttaaagc taattgtccg
    11401 aaatttttgt cggattcaaa tatcatatcc aattgaatac ataattccgg atatatcgtc
    11461 ctgaaatatt gaccatatac aaaatagtga tgaatcgcca ccgagagggc gtccacatct
    11521 gggagtccga ttgctcccat aatctcccta tgtccataat tggctacttt atatagatca
    11581 taccaaaaat ggtcgtccag ctttggaaaa tcaagattct tatacgtagc aatcatgatt
    11641 cgtgcatatt cataaagcac gacattgaaa tattccagag gttgtctgaa ggccggaatg
    11701 atatgcttaa gagaaaacaa tacaatgccg tcttcggaat atagttcaga gccatgatat
    11761 ctatcagggt agaatgggct gagaaatgga ttttgataca ctgcaatcct atcaaatggc
    11821 cgcatcagga aattctcaag attgatggtc aggcgaactg gttcgaaagc gatgatagct
    11881 ctgatatcgt agggcatttc ttcatccatt gacacatatt gccaatcttt atcttcaata
    11941 taaagacata ccctggtatc aaataattcc tttttttcag gaggaagatt tctgtagaat
    12001 tcactgttta tttcgaagat acgttttacc ttatcatcca gtttgggagg aaaacgttta
    12061 taaaaccacc agtctatctg agggccgaga gtgtaccccg taacacctaa aatgacaggg
    12121 atcagcaaat aaggtgaaag ccatgttcca tatacactgt actgatagat gacaattgca
    12181 attgcaagga caaaggcgat caaataagtc aggccagaat atttcatatc aaacattaaa
    12241 tgaaacacaa aactaatcaa acaaatgaaa accatatacg ccgaaaaaag ttggagaggt
    12301 gaaagagttt taaaatgtgt taaggccgga gtggatcttc tttcaggaat ggatctgcaa
    12361 ctatgattac tttgagaatg ctatgaagac attgaaaaaa tattttttta tcgaatatat
    12421 tgtcaaattg aaagtaagtt ctatatttgc atccgctttg gtaccttagc tcagttggta
    12481 gagcaaagga ctgaaaatcc ttgtgtccct ggt
//
LOCUS       DAOQQN010000035        12504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1756_length_12504_cov_5.750261, whole genome shotgun sequence.
ACCESSION   DAOQQN010000035 DAOQQN010000000
VERSION     DAOQQN010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12504
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1756_length_12504_cov_5.750261"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..1505)
                     /locus_tag="PLG24_02310"
     CDS             complement(279..1505)
                     /locus_tag="PLG24_02310"
                     /inference="COORDINATES: protein motif:HMM:NF016693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HMZ23746.1"
                     /translation="MKIEETDVLIIGAGPAGSVAAGILHNHNIRVIMVEKEKFPRFVI
                     GESLLPRCMEAFEEAGFMDDLKRQGYQTKNGALFIRGDDKCDFDFNTQFTRGWNWTWQ
                     VQRAKFDSCMTENLVNRGVDLRFEQTVKAIEFEGSDSTTTVEGKEGNQYKIRARFIID
                     ASGYGRVIPKLFNLDRPSDLPPRMTLFAHVDDPRRGQFQAADRIHAIVQEPHTWIWSI
                     PFSDGTTSVGFVSNPEYFEGLNGSPEENLRALIAREPSVSARFQGNKFLFEPRILKGW
                     SISTDKFFGDGFVLTGNVTEFLDPIFSSGVTLAAASSQLAANIVVRKFRGEQVDWAAD
                     YLAPMLEGVDVFRTYVMSWYNGRLQKIFFSKTIDETIKAQLCSVLAGYVWDYSNPYVR
                     KHKERVESLAAMVGGD"
     gene            complement(1516..2613)
                     /locus_tag="PLG24_02315"
     CDS             complement(1516..2613)
                     /locus_tag="PLG24_02315"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ23747.1"
                     /translation="MSEELDIKVMPVFFENEKMTALEAISEAQRIAFGPVVFQVVRLL
                     RDKGILKVLYDNRENGADEAEISATTGVSEYGVRVLLESGLGAGVCLLKNGRYFISKT
                     GTFILTDKMTRVNLDFVQDVCYKAMWHLEESIEQGKPAGLKELGPWETIYQGLSILPE
                     PALTSWFNFDHYYSDQVFGKIMPVIFMESPKQIFDIGGNTGKFSVACWEYDQDVQITI
                     CDLPVQLANAERFLAQKGCNDRIKGHAVNVLEDNLQFPGKADVIMMSQFLDCFSKEEI
                     VHILKETKQWLNPGGSIFILETYWDRQKFEISAFCLQQTSVYFTAIANGNSKMYHSLQ
                     MKECIEAAGLTVDLEMDNLGISHTLFRCKLQ"
     gene            3264..3521
                     /locus_tag="PLG24_02320"
     CDS             3264..3521
                     /locus_tag="PLG24_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HMZ23748.1"
                     /translation="MDKLQLIERINSFLAEEFEVEESVITPEANIKDTLGLDSLDFVD
                     LVVLIEKHFGFKMAGEDFVSLKNFEDFYNYVFSRLEHKIEA"
     gene            3535..4404
                     /locus_tag="PLG24_02325"
     CDS             3535..4404
                     /locus_tag="PLG24_02325"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HMZ23749.1"
                     /translation="MPEKWTGRSQANSLGFSIFIWTIKHFPLGFSYLLLRLVAIFYLF
                     FSAKANNALIEFYTTLKLNRPYSILRRYATFNLFGQALIDKLAIYLQVGKELSFDFEN
                     EQQLHQLAQEGKGAILLGAHLGNWEIASQLLHRIKVPIYVVLLDNEEKKIKSLIERNS
                     TGKSFSTIPISEDISFVIKIKEALDNGGFVCMHADRYMDGMRVLEAEFLGKGAKFPLG
                     PFYLGTRLKAPAVFVYAIKDRKFHYQFSCSKAYRLKTSEELLHAYVHLLEQMARNHAL
                     QWYNFYPFWDSTH"
     gene            4657..9975
                     /locus_tag="PLG24_02330"
     CDS             4657..9975
                     /locus_tag="PLG24_02330"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ23750.1"
                     /translation="MAQCGINDTFRIPDATTTQHFIEVSGALNNDLSSAQQGICRVNI
                     SFKHPHVGDLRIRLLSPGGQAIVLVGNIGNSYNTTGSFWNVHFVPQSSPSAPDIGIFP
                     IWSNTSNWQAGTSYTGTYYPVAGNQLGQINSGPVNGLWIVEFTDGSMLDEGELISFSI
                     EFCDATGIQCSSCYKSAGVFQISEITACEGSNELKPSLTINYPNGPNSGNTSYSYLVA
                     SADDQIIAVQTIPDLRMFPPGNFTICGIVYDSGHDQYLPIPGTGELLSAWKNMFYNGL
                     PPLCGDMTLSCISVKIKPKSPEKLFTKYICGNGPIYIGNQAITAPGIYTYHYSNSQGC
                     DSALTYTVLPIVFNTSLAVSNEITCTNKKATIRPTSSVPPLTFYQWYDAFGNIQGGVD
                     LDSLIVTVPGTYYLALEKDGCRDTISITVSSDISIPKITIPSVHLDCNMPTAPLNNTT
                     IPPNVGYHWSGPGGFTSTSSTPTVSTPGIYTVTVTKVDGCENTQSVIVSGDFNAPAFN
                     FVIPPKTCSIDTITIGIQTQNSITSFHWELPDGSTQSSPSITTNQNGKIYCTIIAQNG
                     CQKKDSTIINFLTQTPQLITRDTFINCITPIVRLPVSTPLNNFSITWTGPNGFTSNDL
                     SPLINTGGVYTGIATALNNCTTTVTVNVSADFDQPTLSMNSPSFGCKNDSLRINTTFS
                     PANSIFQWTGPKQYVSGDARPFVYETGWYFVTITALNGCTKEDSVFIDINKNNPEITI
                     QDDTITCQMDSVQLLVNVNKPANTTILWTGPNGFQSNDTSPWVSNSGKYWVKVIDKAT
                     GCFTKKSLIIHDITKKPDAVITQDSINCNRNEAIIKLTNDPTYASFYWVTPAGDTLIN
                     TYQFNTIAEDTFHLFIENLYGCKLDTFVHVTPDTVKPVISVTGNYISCSKPSTTLSAT
                     SSTPITSIEWTFPDGTIHTESKPQTNTFGQYSLKVTGPNGCPAFLTFKVVSDTVKPDL
                     KVNGGSFTCKDKSFTLSYTTSIQNGKIQWSGPANFSSSQDNPIVSISGFYKLQLTSPN
                     GCISVDSALVLLTDILPSIQLNNDTITCKNNPVSIIPVTNAINAQYLWLDPSGNQFTS
                     EIMIAKAPGNYILQVQDTNECIVIDTAFIAIDTIKPISLVASEYFINCKNDSLKIIAS
                     SANQNIFTSWYKDQQMVGNDSVYYLTEPGNYRVALESTNGCVVEDTFIVHTDKVVPDF
                     IATGGEINCNFTKITLKVSSLTAGTSFEWNIEGNIVRDTVVTVAKVGLYPLRIKGANG
                     CYFDTLIPVDTNYSIPDLIASDGSLSCDSSNYLLRAISTTPGNTYGWFGPNSFFSDQP
                     NVYAVDTGTYFIFVTGANGCLNVASVYVDDSPPYPGVSLSSDTITCYSPSVPIQLSSP
                     DIIEKQIWTGPDSFTSSERNPTVSAAGVYYVQVSNDYGCTITDSIEIPIDPLSPPVLI
                     TFRDSILCEHREIELYGNQPETGDKYLYQWATADGKILTNPLDSIIRIHNPGNYSLTI
                     INAINGCTSTQTSFIQEKPNPIKSLDVRVEDAECEGINNGEIFVEGGIGTQGTITSNL
                     YGDYFTHHNYFNKLSPGSYFIEIKDEYGCLFGDTVKVGVRDPIHVDLGNDTIIQLGEK
                     ILIDPIFHIDPDRIWSIQWSPVTASCSGCMEFEDSPLVSTLYTVKVTTLGGCISEDEK
                     LVIVQSEDLIFVPNIFSPNGDGHNDFLEFSLAPGVTTIDRISIYDRWGNVVFSRNNLL
                     AGDPAGYWDGTFNGSKVNPGVFIYLIEYRLITGEKLTVKGGVTLLR"
     gene            complement(10356..11429)
                     /locus_tag="PLG24_02335"
     CDS             complement(10356..11429)
                     /locus_tag="PLG24_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ23751.1"
                     /translation="MKNLFTVIFYFLVGLLPLTQVYSQGCKVSNLGFTDVHVDPWNLI
                     VGQEGEIVLPFSAIGCAGVPQYAVLIEVSVTDQLDLGNCVLVNDYGIWETIYYEYANN
                     ATAIWIQNTKGSLDYQINLFDLKIHFTAVTPTSPKGTIVANVGYLNPYCLDWYETNPT
                     DNGDWDEFVVSLPTPINLGPFSANVKDCNSIEINWTTYSETNVDRIEVQKSYDGRNFE
                     TVTAQKSKGNVIVKSNYSVVDTAPLQPGKVYYRLKTIDLDGSISYSRIIPVTVNCGLK
                     KDIIVYPNPTYGPTSVQFSGFKAGSALKVALMNAQGQVLRTMALDPVEKNSLDLSDYP
                     GGVYFLKVLDDQSTLRAKIIKVN"
     gene            complement(11782..>12504)
                     /locus_tag="PLG24_02340"
     CDS             complement(11782..>12504)
                     /locus_tag="PLG24_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ23752.1"
                     /translation="REAKYQIRVKFEVAKESCTDCVAIVGNLGLDDAAERISETEPSN
                     NGKVSYFTAHGSLPITLGRFSAAINDCSDVNIDWETKGENDVERIEVERSYDGISFLS
                     TKAIKAKGKRKVSSTYAVMDETAIQRGKVYYRLKTVDNKGAIAYSKVLDVNVDCDQKP
                     EMRVYPNPAYGHTTVTFKGIRNMPGIEVALLNDQGNMVRTLKMDPSGANVVNLEALPS
                     GAYFIKVLDDKIDLKSKFIKVD"
BASE COUNT         3743 a         2819 c         2212 g         3730 t
ORIGIN      
        1 atgcccttcc cttgaactag gtagatgatt aatagccgca atggcaatga tatcgggcat
       61 gatcattact acatcccaaa aacttaccca aatcaagtcg ttttaatttt ggcaacctca
      121 tggaaagcta atgatagctg gttttcaagt tattactttc ctacctactt cggtttttcc
      181 aacctccccc ctttgaaaga caggcatatc ttcacatagc cataatctat gcccaaacct
      241 tgcatactca gataaatcca caaaaaataa ccggaatctt agtcaccacc aaccatggca
      301 gcaagacttt ccaccctttc tttatgttta cggacataag gattggaata atcccataca
      361 taacccgcca acacactaca caattgggcc ttgatggttt cgtcaatagt ctttgaaaag
      421 aatattttct gcaatcgtcc attataccag gacataacat aggttcggaa tacatctacg
      481 ccttcaagca taggcgcaag gtagtcggcg gcccaatcga cctgctctcc cctgaatttc
      541 ctgactacaa tattggcggc aagctgtgaa gaagcagctg caagtgtcac accacttgaa
      601 aatatcggat ccaaaaactc tgtcacattc ccagtaagta cgaagccatc gccaaaaaac
      661 ttatccgttg agatactcca acccttaaga attctgggct cgaacaaaaa tttatttcct
      721 tgaaaacgcg cgcttacaga tggctcccgg gctataagag cacgaagatt ctcttcaggg
      781 ctgccattta gaccttcaaa atattccgga ttacttacaa aacctacaga tgtagtacca
      841 tctgagaatg gaattgacca gatccaggta tgcggttcct ggactatcgc atgtatcctg
      901 tcagctgcct gaaactgacc tctccttgga tcatcgacat gtgcaaataa agtcattctg
      961 ggtgggagat ctgaaggccg atctaaattg aataatttag gtatgactct cccatagcca
     1021 ctcgcatcaa tgataaatct ggccctaatc ttatattgat ttccttcctt tccctctaca
     1081 gttgtggtag aatcacttcc ttcaaattca atagccttga cagtctgctc aaatcgaaga
     1141 tccacacctc tgttgacaag attttctgtc atgcatgaat cgaatttagc cctttgtacc
     1201 tgccaggtcc agttccaacc tcgggtaaac tgagtgttga aatcaaaatc acatttatca
     1261 tcacccctta taaatagagc gccattttta gtctggtaac cttgcctctt aagatcatcc
     1321 ataaatcctg cctcttcaaa agcttccata catcttggta aaagactttc accaatcaca
     1381 aaacgtggaa acttttcttt ttccaccatg ataactctta tattgtggtt gtgaagtatt
     1441 ccggctgcaa cactccctgc cgggcctgca cctattatca gaacatccgt ttcctcaatt
     1501 ttcattttac ttgttttatt gtaatttaca tctgaaaaga gtatgactaa tgcccaggtt
     1561 gtccatttca agatcaaccg tcaatcctgc agcttcgata cattctttca tttgcaggga
     1621 atggtacatc ttactgttgc cattggcaat ggctgtaaaa taaacactgg tctgttgaag
     1681 gcaaaaagct gatatctcaa atttttgacg atcccaatat gtttccagaa taaaaatgct
     1741 tccacccgga ttcaaccatt gttttgtctc tttaagtata tgtactatct cttccttgga
     1801 aaaacaatcc aaaaattgac tcatcatgat gacatccgcc ttgcccggaa attgaagatt
     1861 atcctcaagc acattgacag catgcccctt gatcctgtca ttgcacccct tttgcgctaa
     1921 aaatctttca gcgtttgcca gttgcaccgg aaggtcgcaa atagtgattt gtacatcttg
     1981 atcatattcc cagcaagcta cagaaaattt gccggtattg ccaccaatat cgaatatttg
     2041 ttttggcgat tccatgaata ttaccggcat aatcttccca aaaacctgat cggaatagta
     2101 atggtcgaag ttaaaccatg aagtaagagc cggttcaggt aggatactca agccctgata
     2161 gatggtctcc catggcccca actccttgag tccggccggc ttaccttgct cgatcgattc
     2221 ttcaagatgc cacattgcct tataacatac atcttgtaca aaatccaaat tgacccgcgt
     2281 cattttatcc gtcaaaatga atgttccggt cttcgaaata aaatatctgc catttttcaa
     2341 cagacatacg cctgccccca atcctgattc gagcagtacc ctgacgccat attctgaaac
     2401 acctgttgtt gcggaaatct ctgcttcgtc tgcaccattc tctctgttat catagagaac
     2461 ctttaaaatt cccttatctc tgagaagtct aaccacctgg aagacaaccg gaccaaatgc
     2521 aatccgctgt gcttcggaaa tagcttccaa tgcagtcatc ttttcatttt caaaaaatac
     2581 aggcatgact ttgatatcca attcctcact catccattct tgtttttaaa atttctcgta
     2641 aaaataagta atgtccccca aaaattgcct ttgtcttcga tgttaatcta aataatgaaa
     2701 atattaatac atatttctcc atttctaaat gttatagaaa agtgtccgat tcattcaaat
     2761 aacagggctg ttttgcgaaa aatacggtat agattatgaa aaaaaacgat tctacctcac
     2821 caggatttcc tgcaaatttt aaggaaaatt ctgatgttgc ttgcataaac ctttgtatct
     2881 cacaaattgt acttttcatc tatccggaag acaagttgag tcatttctag taaggaagct
     2941 ataggatgct agatattaac aacttgatat tatgtttttt atacatatta atcatttgta
     3001 acctattcgc gaaataagct gaattaatca agacgggtta tatttctata caatactccc
     3061 acaatgaata acccaaaata cctgactaaa attaaatttc ctccggctca tgacccgatt
     3121 aaattgcagg tgacagccga gacccgtccg atacagcgtt tgtcccttgc aaatatttat
     3181 ttgtatccgg ttggcaaatt tgtgacctac atcagatacc caatcataag cgaaataaat
     3241 aaattattat ttatatttgc cccatggaca aattacaatt aatagagagg atcaattcat
     3301 ttctcgcaga agaattcgag gttgaggaat cggtcatcac ccctgaagcc aacatcaagg
     3361 acactcttgg tttggatagt cttgacttcg ttgatttggt tgtgttgata gaaaagcatt
     3421 ttggttttaa gatggcaggt gaggactttg tatcattaaa gaatttcgaa gatttttaca
     3481 attacgtttt ttcacgcctg gagcataaaa tagaggcctg ataatccata atttatgccg
     3541 gaaaaatgga ccgggagatc ccaggcaaac tctctgggtt tctcaatttt tatatggaca
     3601 atcaagcatt ttccgcttgg tttctcctat ttactactgc gcttagtagc aatattttac
     3661 ttatttttct cagcaaaggc caataacgca cttattgaat tctatactac actgaagctt
     3721 aatcgtcctt attcaatact caggaggtat gccacattca atcttttcgg tcaggctttg
     3781 atcgataagt tagcgatata tttacaagtt ggcaaggaat tgtcttttga tttcgaaaac
     3841 gaacaacaac tccaccaact ggctcaggaa gggaagggag ctattttatt aggtgcccac
     3901 ctgggcaact gggagattgc aagccaattg ttacacagaa ttaaggtgcc tatatatgtt
     3961 gtcttgctgg acaatgaaga aaaaaaaata aaaagcttga ttgaaagaaa ttcgactggc
     4021 aaatcatttt caaccattcc gatttcagaa gatatttcat tcgtgatcaa aattaaggaa
     4081 gccctggata atggtggctt tgtgtgtatg catgctgata gatatatgga tggtatgcga
     4141 gtactggaag ccgaattttt aggcaaaggt gcgaaatttc cgttaggacc tttctacctg
     4201 ggtacacgat tgaaagcccc tgctgtcttt gtatatgcca tcaaggaccg aaaattccat
     4261 tatcaattca gttgtagtaa ggcatatcga ctgaaaacgt cggaagaact actacatgct
     4321 tatgtgcatt tacttgagca aatggcacgt aatcatgcct tgcagtggta taatttttat
     4381 ccattttggg attcaactca ttgatcaagt taaaggaaac aaattttaaa tgattattgc
     4441 ggaaaataaa ttactatcag attttacctt atctttacac aattagcctc acccacaaat
     4501 tcacactaat taaaaaaatg tagggcaata ataatattaa ataaagaatt tttaccttta
     4561 tttgattttg cgacctttac cttgattatg acgttcaaaa ggcttaatat aagattgatt
     4621 ctctgtgttg taaatatatt tttttacaat atacttatgg cgcaatgtgg aataaacgac
     4681 acctttagaa ttcctgatgc tacgacaact caacacttta ttgaagtatc tggcgcattg
     4741 aacaatgact taagtagtgc ccaacaaggc atttgtcgtg tcaacatcag cttcaagcat
     4801 cctcacgtag gcgatcttcg tatccggttg ttgagtccag gcggacaagc cattgtctta
     4861 gttggcaata ttgggaacag ctacaataca accggaagct tttggaatgt tcattttgtt
     4921 cctcagtcta gtccttctgc ccctgatatc ggtatctttc cgatttggtc caatacttca
     4981 aactggcaag ccggcaccag ctatacaggt acctattatc cggttgcggg aaatcagctg
     5041 ggtcaaataa actccggacc tgtcaatggc ttgtggattg ttgagtttac cgatggatcg
     5101 atgctcgatg aaggagagct tatttccttt tccatcgaat tttgtgacgc tacaggaatc
     5161 caatgctcat catgttacaa atccgcagga gtatttcaga tttctgaaat aactgcctgt
     5221 gaaggcagca atgagttgaa gccttctttg acgataaatt atcctaatgg tcccaatagt
     5281 ggaaatactt catatagcta tctggtcgcc tcagctgatg atcagatcat tgcagtccag
     5341 acaattccgg acttaaggat gtttcctccc ggcaacttta ccatatgcgg tatcgtctat
     5401 gacagcggcc atgaccaata cttacccatt cccggaacag gagagttgct atctgcatgg
     5461 aaaaatatgt tctacaatgg cttgcctcca ttatgtggag acatgactct ctcgtgtatt
     5521 tcggtaaaaa ttaaacccaa aagtccggaa aaactattta caaaatatat ttgcggcaat
     5581 ggaccgatat atatcggcaa ccaagccatt acagctcccg ggatttatac ctaccattat
     5641 tccaacagtc aaggctgtga ttcagcccta acctatactg tcttacccat cgttttcaat
     5701 acatcccttg ctgtttcaaa tgaaataaca tgcaccaata aaaaagctac catcagaccg
     5761 acttcatcag tacctccttt gacattctat caatggtatg atgcgtttgg gaacattcaa
     5821 ggaggagtcg atctggattc ccttatcgtt actgtgccgg ggacctatta cctggccttg
     5881 gagaaagatg gatgtcggga tacaatttcg attacggttt cttcagatat ctccatcccg
     5941 aagattacta taccttcggt acaccttgat tgtaacatgc ccaccgctcc actcaataac
     6001 acaactattc cgcctaatgt aggttaccat tggtctggac cgggtggctt tacctcaacc
     6061 tcctccacac caactgtatc aacccccgga atatatacag tgactgtaac taaggttgat
     6121 ggatgtgaaa atacacagag cgtaatcgtg tcgggtgatt ttaatgctcc tgcttttaac
     6181 ttcgttattc cacccaaaac ttgttcgata gatactatta caataggaat ccagacacaa
     6241 aacagtatca ctagttttca ttgggaatta ccggatggaa gcactcagtc aagcccgtca
     6301 ataacgacta atcaaaatgg caaaatatac tgtaccatca tagctcaaaa cggatgccag
     6361 aaaaaagaca gcactataat caacttcctt acacaaaccc ctcaattaat taccagagat
     6421 acctttatca actgcattac gccaatagtc cggctaccgg tgagtactcc attaaacaat
     6481 ttttccatta catggacagg tccaaatgga ttcacttcga atgatctaag ccccctaatc
     6541 aacaccggag gggtatatac aggtattgca acagcattaa acaattgcac tacaaccgta
     6601 acagtcaatg tttcagcaga ctttgaccaa cctactctta gtatgaactc accatctttt
     6661 ggttgtaaaa atgacagttt aagaatcaat actaccttta gtccggcaaa ttctatcttc
     6721 caatggacag gacccaaaca atatgtttca ggggatgcaa ggccatttgt ctatgaaaca
     6781 ggctggtatt ttgtaaccat tactgctctc aatgggtgca caaaggagga tagcgtattc
     6841 atcgatatca acaagaacaa tccggaaata acaattcagg atgataccat tacatgccaa
     6901 atggacagtg ttcaattgtt ggtcaacgtc aacaaaccgg ccaatacaac tattctgtgg
     6961 acaggcccca atggctttca atctaatgat acatcgccat gggtttccaa ttcgggtaaa
     7021 tattgggtaa aggtcattga caaggctacc ggttgtttta ccaaaaaatc gctcatcata
     7081 catgatatta ccaaaaagcc tgatgccgtc attactcagg atagcatcaa ctgcaatcgc
     7141 aatgaagcta taataaagct gacaaatgat ccaacgtatg cttcatttta ctgggtaact
     7201 cctgccggcg atactttaat aaacacttat caattcaata ccattgctga ggataccttc
     7261 cacctcttta ttgaaaatct ttatggatgt aagttagata cctttgtgca tgtcactccg
     7321 gatacggtga agccggtgat ttcagtcacg gggaattata tttcctgctc taagccatca
     7381 actactcttt cggcgacgtc ctccactcca ataacaagta tcgaatggac atttcccgat
     7441 gggacaatcc acaccgaatc aaaacctcaa acgaatactt tcggccaata tagtctgaag
     7501 gtgaccgggc ccaatggatg ccccgccttt cttaccttta aggttgtttc cgataccgta
     7561 aagcctgact taaaggtcaa tggtggtagt ttcacttgta aggacaaatc attcacccta
     7621 tcctacacca cttctatcca gaatggaaaa atccaatgga gcggaccagc caacttctca
     7681 tcttcacagg acaatcctat tgtttccatc tcgggattct acaaactaca gctcacttct
     7741 cccaatggtt gcatttcagt tgattcagca ttggttttac tgactgacat tctaccgagc
     7801 atacaactta ataatgatac cataacctgc aaaaacaatc ctgtctcaat aataccggtt
     7861 actaatgcca ttaatgcgca atacctatgg ttagatccgt ctggtaatca atttacatcc
     7921 gagataatga tagccaaagc tcccggcaac tatatccttc aagtacagga cacgaatgaa
     7981 tgtattgtca tcgatacggc ttttatcgct atcgacacca tcaaacctat atctttggtt
     8041 gcctccgaat attttatcaa ctgtaagaac gattcattga aaataatagc ctcgtcagcc
     8101 aatcaaaata tcttcacctc atggtataaa gaccaacaaa tggtaggcaa tgacagtgtt
     8161 tattatctta cggaacccgg caattaccgg gttgctttag agagcacaaa cggctgtgtt
     8221 gtagaagaca cctttattgt acatactgac aaggtggtac cggattttat tgcaacaggt
     8281 ggtgaaatca attgtaattt caccaagatt acattaaagg tttcatcact tactgccggc
     8341 acttcattcg aatggaatat cgagggtaat attgtcagag acacggtagt tacagtggcc
     8401 aaggtaggtt tatacccttt gcggatcaaa ggagccaacg gatgttattt tgatactttg
     8461 atccctgttg atactaatta tagtatccct gacctgattg catccgatgg ctccctttcc
     8521 tgtgattcaa gtaattactt actgagagcg atttcaacaa ctcccggaaa tacatatggc
     8581 tggtttgggc caaactcctt cttttcagat cagccaaacg tatatgctgt cgataccgga
     8641 acttatttca tatttgtaac cggcgccaat ggttgcctca acgttgcttc tgtctatgtc
     8701 gatgacagtc ccccataccc aggtgtatcc ctatcatccg acaccatcac ctgttattct
     8761 cccagtgtgc ctatacagct cagcagccct gatatcattg agaaacaaat atggacagga
     8821 ccagattcgt ttacttcatc agaaaggaat cctactgttt cagcggcagg agtgtattat
     8881 gttcaggtca gcaacgacta cggatgcact attactgatt cgattgaaat accgatcgac
     8941 cccctatcac cgccagtgtt gatcacattc cgagactcca ttctttgcga acacagagaa
     9001 attgaactat acggcaatca gcctgaaacc ggagataaat acttatacca atgggcaacc
     9061 gctgatggca aaattcttac aaacccgctt gatagtatca tccgcatcca caaccctggt
     9121 aattacagct tgacaatcat taatgcaata aatggttgca cgtccacaca aacatccttc
     9181 atccaagaaa agcccaatcc tataaaaagc ctggatgtac gggttgaaga tgccgaatgt
     9241 gagggcatca ataacggaga aatctttgtt gaaggaggaa ttggaactca gggtactatt
     9301 acctccaatc tttatggtga ctactttaca catcataatt acttcaataa gctgagtccc
     9361 ggatcctatt tcatcgaaat taaagacgaa tatggctgcc tctttggtga tacagtaaag
     9421 gtgggtgtca gggatcctat ccatgtcgat ctgggcaatg acaccatcat tcaactggga
     9481 gaaaagattc ttatcgatcc gatattccat atcgatccgg atcggatatg gtccattcag
     9541 tggtcccctg ttactgcctc ctgctccgga tgcatggaat tcgaggactc gccccttgtc
     9601 tcgactttat atacagtcaa ggttacgacc ttaggcgggt gcatatcaga ggatgaaaaa
     9661 ttagtgatcg tgcaaagtga agatctgatc tttgtaccca atattttctc tccaaatggc
     9721 gatggccata acgatttcct tgaatttagc ctcgcaccgg gtgtaaccac aattgaccgg
     9781 atatcgatat acgatagatg gggcaatgta gtattcagcc gcaacaatct attagccggc
     9841 gatccggcag gttattggga tggaacattc aatggcagca aggtaaatcc cggagtattc
     9901 atttacctga ttgaatatcg tttaataaca ggagaaaagt tgaccgtcaa gggtggagtg
     9961 actttattga gatgatctca atcgcattta tttcatttat aatattgata atcaagaaat
    10021 gatctttttg cctgcaatcg gtacgctcca tgattagtgc attctgcgca tcatcaatgt
    10081 aatgcagccg gaaagttatt tcagaaatcg cctcattacc tgcaaaacta tgattaatga
    10141 atgtgttttc aatacataaa tttgattaca aatattatac atatgcaatt aaattaatat
    10201 ttaaatagtc ttcgtacaaa ttgatcgatt tattgaaaat ttacagacta tattgcttcg
    10261 atggaaatct aaataaaaag ggtgcatctc tgcacccttt ccattaaatt tgcttcactc
    10321 taaaataaac aatcaacaat gaaaattaga aatcatcagt tgaccttaat tatcttcgcc
    10381 cttagggtac tctgatcgtc taacaccttg aggaaataga ctcccccggg ataatctgaa
    10441 agatccaggc tgttcttttc gacaggatcc aacgccatag ttctcaagac ctgaccttgg
    10501 gcattcatca gagcaacctt aagagctgat cctgccttga agccactgaa ctgcaccgaa
    10561 gtgggtccat aagttgggtt gggatatact attatatcct tcttaagacc acaattaacc
    10621 gtgacaggga taatgcggga atagctgatg cttccatcca gatctatagt tttcagacga
    10681 taatatacct ttcccggttg caatggagcg gtgtccacaa cagaataatt actttttact
    10741 ataacatttc cttttgattt ctgtgctgtg acagtctcaa aattccttcc gtcataagat
    10801 ttttgcactt caatcctatc tacattcgtc tcactataag tagtccagtt gatctcaata
    10861 ctattacaat ccttgacatt ggcggagaat ggacccagat tgatgggggt tggaagagaa
    10921 acaacaaatt catcccaatc gccattatca gttggattag tctcatacca atcaagacaa
    10981 tacggattaa gatagcctac atttgccaca atcgttcctt tgggactagt gggagtgaca
    11041 gcagtaaagt gaattttcaa atcaaaaaga ttaatctggt agtccagtga gccttttgtg
    11101 ttttgaatcc aaatagcagt agcattattc gcatattcat aatatatagt ttcccatata
    11161 ccataatcat tcaccagcac acaattgccc aaatctagtt gatcagttac actcacttca
    11221 ataagaacag catattgagg cacaccggca cagccgatag cgctaaatgg taaaacaatt
    11281 tcaccctctt gacctacaat cagattccag gggtctacgt gaacatcggt aaatcccagg
    11341 ttagaaactt tacacccttg tgaatatact tgtgttaaag gaagtaatcc tacaagaaaa
    11401 taaaaaataa ctgtaaataa atttttcata gattataatt ttaaagtgaa atttaatttc
    11461 atatttaatt ttactaagga ttccttcata aggtctattg acatactact cctccataca
    11521 tgacaatcaa ttaatcttta tctgaggcct tgcatttgtt ctgagctctt caataaaatt
    11581 attccatgtt gagtcttgat attgttagaa tcaaattccc ttttattctt actcagctta
    11641 attgattact ctcgatagtc gcgtctttcc cggaataatt tcatctttaa tccacaaaat
    11701 gaatatggaa gaaatgaaag aaattgcctt tttcattacc ttttcttcca tattcagatc
    11761 aacagcataa ggtggtggtt atcaatccac cttaatgaac ttcgatttca aatcgatttt
    11821 atcgtccaat accttgatga agtaagcgcc acttggcaag gcctccagat tgaccacatt
    11881 cgctcccgat ggatccatct taagcgtcct gaccatatta ccctgatcat tcagcaatgc
    11941 tacctctatt ccaggcatat tcctgattcc cttgaatgtt accgtagtgt gcccatatgc
    12001 cggattggga tataccctca tctcaggttt ctggtcacaa tctacattca catccaaaac
    12061 tttagaataa gcaattgcgc ccttattatc aactgtttta agcctgtaat ataccttacc
    12121 ccgctgtatt gctgtctcgt ccatcactgc ataagtactt gacaccttcc tcttcccttt
    12181 tgcctttatt gctttcgttg aaagaaatga aatgccatca taggatctct ccacttcaat
    12241 acgttcgaca tcattttctc ccttcgtctc ccagtcaata ttgacatcag aacaatcatt
    12301 gattgcggcg ctgaacctgc caagcgttat cggcaatgag ccgtgtgcag taaagtaact
    12361 cacctttcca ttattggatg gctctgtctc agatatccgt tctgccgcat catctaaccc
    12421 taagttccct actatggcta cacagtcagt acaactttcc ttcgccactt caaacttaac
    12481 ccggatttga tatttagcct ctct
//
LOCUS       DAOQQN010000036        12374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1782_length_12374_cov_5.539005, whole genome shotgun sequence.
ACCESSION   DAOQQN010000036 DAOQQN010000000
VERSION     DAOQQN010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12374
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1782_length_12374_cov_5.539005"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PLG24_02345"
     CDS             <1..335
                     /locus_tag="PLG24_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HMZ23753.1"
                     /translation="RHGEGKWILKKAMEPLLPHHVIYRPKTGFGAPLRRWLHVEMKEL
                     LESDKAIVVLRANVTSTTSNQLALGTATTNDYLVAINAEDQARQNMLLHQIQLLMIGY
                     HLKTITGN"
     gene            376..1296
                     /locus_tag="PLG24_02350"
     CDS             376..1296
                     /locus_tag="PLG24_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HMZ23754.1"
                     /translation="MAKMIRKNTIAAGIMIACMIVGIVSCNKDKDNFDASGSFEAIET
                     IVSAEANGRIERLEIEEGQELEAGRNIGFIDSTQLFLKKKQLEAQIVAILGRKPDIPV
                     QLSALQEQLRTAERERVRTVGLVKGEAATAKQLDDLDAQIALLKKQIEAQKSTLSISS
                     TGLSKEAMPLQWQIAQVNDQLTKCRIVNPVKGSVLAKYAEAKEVTAAGKPLYKIADLS
                     EIILRTYITGDQLSRVKPGQKVKVLTDDGKGGYDTGEGTIIWINDKAEFTPKTIQTKN
                     ERANMVYATKIKVKNDGRYKIGMYGEVLFQ"
     gene            1356..2342
                     /locus_tag="PLG24_02355"
     CDS             1356..2342
                     /locus_tag="PLG24_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ23755.1"
                     /translation="MVILNNISKTYNDGRVIAVNDVSFDVNKGELFGLIGPDGAGKTS
                     IFRMLTTLLLPDSGTATVNGRDVVKDYKYIRNNVGYMPGKFSLYQDLSVEENLQFFAT
                     IFNTTVEENYDLVKDIYVQLEPFKRRRAGKLSGGMKQKLALCCALIHRPKVLFLDEPT
                     TGVDAVSRKEFWAMLQSLKEQGITILVSTPYMDEAMLCERIALLQKGNIMSIDTPENI
                     ISTFPDALYAVKAHGMSRLQDVLRNDPGVNSCYSFGDYHHITFTEGNNADDPEEIREV
                     HDKTEFDHFPEYPHVSALLRRIQHEGFQDLEIKRISPGIEDCFIHLMENKLH"
     gene            2360..3148
                     /locus_tag="PLG24_02360"
     CDS             2360..3148
                     /locus_tag="PLG24_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ23756.1"
                     /translation="MEGTKTGVAENKFLSASEVVIRTDKLTKRFGDFVATNEITFEVY
                     AGEIFGFLGANGAGKTTAMKMLCGLSRPSSGKAVIAGYDIYKDTERIKKKIGYMSQKF
                     SLYEDLTVMENIRFFGGIYGLSNKHLQKKSHELIETLNLQNEANLMVTSLPLGWKQKL
                     AFSVAVMHEPRIVFLDEPTGGVDPITRRQFWDLIYSTADKGITIFVTTHYMDEAEYCD
                     RVSIMVDGVIEALDTPANLKRRYSVASMDEVFYHLARGAKRSVD"
     gene            3246..4352
                     /locus_tag="PLG24_02365"
     CDS             3246..4352
                     /locus_tag="PLG24_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ23757.1"
                     /translation="MKRFFSFVRKEFYHVFRDGKTMLMLFGMPVMQILLFGFALTNEI
                     KNSQIVICDYSRDDATRKIISKLEASNIFHIRESLLSHMQIEESFKSSNVKLVVIFPN
                     NFNHDLLHSNKAQVQIIADASDPNTATTLTNYVTSIIMTYQQQLMAINAFPMSITPEI
                     RMYYNPELKGATNFVPGVIALVLMLICVMMTSVSIVREKENGTMEILLVSPFNPMLVV
                     ISKAIPYLFLSIVNLLIILILSVSLLDMPINGSIFLLIAESILFIITGLSLGLLISNL
                     VESQQAAMLISMMGMMVPTILFTGFMFPIENMPIPLQIISYFIPSKWFYTIVKAIMIK
                     GLGFYDVWKETLVLAGMVVVLLTISFKKFKTRLA"
     gene            4349..5470
                     /locus_tag="PLG24_02370"
     CDS             4349..5470
                     /locus_tag="PLG24_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ23758.1"
                     /translation="MRTLKFLLQKEFKQIFRNRAILPLIFVVPVVQLLILPLAADYEV
                     KNINISVVDGDHSTNSRQLIAKITSSGYFRLTGYGTSFDEAYGLMESDDSDLILEIPA
                     GFERNLVREGEQKVFVAVNAINGVKASLGGAYLMRIISNYNDDIRLDWIQKPRFNPSP
                     QIEVTSSNWFNPHLDYNFFMVPAILVILVTMVGSYLCALNIVKEKEMGTIEQINVTPI
                     RKYQFVLGKLIPFWVIAMFVFTLGLFVVAYWVYGIVPLGSWVVLYSYLAVYLIAMLGL
                     GLLISTFSETQQQAMSISFFFMMIFMLMSGLFTPIDGMPEWAYVIARSNPVTYCIEVV
                     RMVVLKGSGFNDLKQHFLITAVFAVALNSFAIWNYKKTS"
     gene            complement(6260..6898)
                     /locus_tag="PLG24_02375"
     CDS             complement(6260..6898)
                     /locus_tag="PLG24_02375"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HMZ23759.1"
                     /translation="MGKIKISAAEKADPLIHPFEAGSGIEAGVDEVGRGCGAGPVVAA
                     AVILPPKAKIPGLRDSKKLTASKRKELSGKIRELALDYSIAISTVEEIDQINILEATY
                     LAMTRAIAQLKIKPDLLVIDGNRFKNQTGINHRTVVGGDDKVLSIAAASVLAKTWRDA
                     YMEELHIEFPQYDWINNKGYLSTKHRQACFTHGLTPHHRKSFKGMTRQDDET"
     gene            complement(6886..9171)
                     /gene="bglX"
                     /locus_tag="PLG24_02380"
     CDS             complement(6886..9171)
                     /gene="bglX"
                     /locus_tag="PLG24_02380"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HMZ23760.1"
                     /translation="MTVLRSLYLLCFLLISQVSFGQKGKPDRMKVFIDELMSKMTLTE
                     KLGQLNLPVAGDITTGQASSSDIGKKIREGNVGGLFNIKSVEKIREVQRIAVEESRLK
                     IPLLFGMDVIHGYETVFPIPLGLSCSWDMDAIRRSARIAAIEASAAGINWTFSPMVDL
                     CRDPRWGRISEGNGEDPYLGSAIAQAMVRGYQGTLTENNQILACVKHFALYGAAEGGR
                     DYNTTDMSRIRMYNEYFPPYQAAVIAGVGSVMASFNEVDGIPATGNKWLLTDVLRKQW
                     GFKGFVVTDYTGINEMIDHGIGDLQTASAMALNAGIDMDMVGEGLLTTLPKSLKEGRV
                     TMAKIDAACRLILEAKYKLGLFEDPYKFCDEKRSKTEIFTPEHRAEARKIAAESFVLL
                     KNNGDLLPLRKSGTIALVGPLAHAQSNMTGTWSVAAVNDKSITLRQGLERALAGKATL
                     RYAKGSNLTEDAALEERGTMFGKSLDRDNRSDEELLREALEAAHSSDLIIAALGESAE
                     FSGESSSRTNLNIPDVQKKLLMELVKTGKPVVLVLFNGRPLTLTWEDAHVPAILDVWF
                     GGSEAGDAIADVLFGDVNPSGKLTTTFPQNVGQIPLYYNHKNTGRPLQEGHWFQKFRS
                     NYLDASNDPLYPFGFGLSYSKFRYSDIKVSSTSLKGNQKLTASVEITNVSDFNAKEVV
                     QLYIRDVVGSVTRPVKELKGFKKVEIGARQTVTVSFEITPEDLKFYNYDLQFDWESGD
                     FDIMIGANSRDVSKTRIYWEK"
     gene            9574..10581
                     /locus_tag="PLG24_02385"
     CDS             9574..10581
                     /locus_tag="PLG24_02385"
                     /EC_number="5.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyproline epimerase"
                     /protein_id="HMZ23761.1"
                     /translation="MSVSKKFFCVDAHTCGNPVRLVAGGGPALIGANMSEKRQHFLSE
                     YDWIRKGLMFEPRGHDMMSGSILYPPHDPANDVAVLFIETSGCLPMCGHGTIGTITIA
                     IEEGLIRPREAGKVRMETPAGLVFVTYHQEGKKVKSVKLINVPAYLDSEGLEAECPDL
                     GMLRVDVAYGGNFYAIVDVQENFAGIEHYTADKLVAWARALRRNINERYSFVHPEDDT
                     IRGCSHILWTGKILDPNSTARNAVFYGDKAIDRSPCGTGTSARMAQWYTKGLLKPGDR
                     FVHESIIGSKFVGTIEEVTTVAGRPAIRPGIEGWARIYGYNTISIDDEDDPFAFGFQV
                     I"
     gene            10630..11877
                     /locus_tag="PLG24_02390"
     CDS             10630..11877
                     /locus_tag="PLG24_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ23762.1"
                     /translation="MKAVIIGGGIIGLSSAFFLKSSGWDVIVVDQGDMTHNCSYVNLG
                     MVVPSHFVPLAAPGMVSQGIRWMFDRKSPFYVRPTLSGRLISWGLKFMRSANDSHVSN
                     AAEPLRDLSLLSRSIFDTWAAMPELDFGYERKGIIMYFNSSKTGDEEVHLAEKAVKLG
                     LDAIVLGPQELSRLEPAVQPNVMGGVHYRCDGHLNPDRLMRQLMGLLADKGVLIERNR
                     EVIGFTQNHGRITSVKTKEGAMDTDLVVLANGSWLPDLAKMVGMNIPLMPGKGYSFMQ
                     ANPPAQLNIPAILCEARVAITPMGGGIRFGGTMEIDRIREGGVNMNRVAGIVESLPRY
                     FDNFRVEMPDMHQVSYGYRPCSPDGMPYIGSSRRHDNLLVAGGHAMMGLSLGPVTGKL
                     IAGLANGEKPEIDLKPFDPNRFD"
BASE COUNT         3419 a         2394 c         3003 g         3558 t
ORIGIN      
        1 acaggcatgg cgaaggtaag tggatactca agaaagcgat ggagccattg cttccccacc
       61 atgttattta tcgccccaag actggatttg gagccccgtt gagaagatgg cttcacgtcg
      121 agatgaaaga gctacttgaa tcagacaaag caattgtcgt actgcgtgct aacgtgacaa
      181 gtactaccag caaccagctt gcattgggca cggccactac caatgactat ctcgttgcaa
      241 tcaacgcgga ggatcaggcg cggcaaaata tgttgctaca ccaaatccaa ctattgatga
      301 taggatatca tttaaaaacc ataacaggca attgaaggca ggatgtgtaa ttctaaaaat
      361 caaaaattaa caaaaatggc gaagatgata aggaagaata caatagcagc aggtataatg
      421 attgcctgta tgattgtggg gatagtatcg tgcaacaagg acaaagataa cttcgatgct
      481 tcaggaagtt ttgaggcgat agaaaccatt gtctcagcag aggcaaatgg aagaatagag
      541 cgtttggaaa ttgaagaagg acaggaattg gaagcaggga ggaatatagg cttcattgac
      601 agcacacagc tttttttaaa gaaaaagcag ttggaggcac agattgtcgc tatacttgga
      661 aggaagccgg acattccggt tcaactatct gcattgcagg aacaactcag gacagccgag
      721 cgtgaacgtg tgaggactgt cggtttagtc aaaggtgagg ccgccacagc caagcaattg
      781 gatgacctcg atgcgcagat agctttgctg aaaaagcaga tagaggcgca aaaatctacc
      841 ctgagtatat cgagtacggg actcagcaag gaagccatgc ctctacaatg gcaaatcgcc
      901 caggtgaatg accagttgac caagtgccga attgtcaatc cggtcaaagg ctcggtatta
      961 gccaaatacg cagaagcgaa ggaggtgact gctgccggta agccactata taagatcgcc
     1021 gacctttctg aaattatatt gcgcacttac atcacagggg atcagctgtc gagggtgaag
     1081 ccggggcaga aagtgaaggt gctgacggat gatggcaagg gcggttacga cacaggtgaa
     1141 ggcaccatta tctggattaa tgacaaggct gaattcacac ctaagaccat tcagacgaag
     1201 aatgagcgtg ccaatatggt gtatgcaact aagataaaag tcaaaaatga cggtagatac
     1261 aaaatcggaa tgtatggcga agtcctcttt cagtgaagaa ttattgatag gtattgacaa
     1321 gaaaaacgct ggattattca gaaaaataaa ccgacatggt gatactcaac aatatatcaa
     1381 agacatataa cgatggtagg gtgatagctg ttaacgatgt ttcctttgat gtcaacaaag
     1441 gcgaattatt cggacttatc gggccggacg gtgcgggcaa aaccagtatc tttcggatgc
     1501 tcaccacgtt gttgctccct gatagcggaa ctgccaccgt aaatggccgg gatgtggtga
     1561 aggattacaa atatattcga aacaatgtgg gttatatgcc cggcaaattt tcattgtatc
     1621 aggatctgtc ggtagaggag aatctgcagt tttttgcaac aatatttaat acaacggtgg
     1681 aggaaaatta cgatttggtg aaagacattt acgtccagct cgagccgttc aaaagaagga
     1741 gggcagggaa attatcaggt ggaatgaaac agaagcttgc cttgtgttgt gcattaattc
     1801 atcggccgaa agtgctgttt ctggatgaac cgactaccgg tgttgatgcg gtgtcgagga
     1861 aagaattttg ggcgatgtta cagtccctta aagagcaagg aatcaccatc ttagtatcta
     1921 cgccctatat ggatgaggcc atgctttgtg agcgaatcgc acttttgcag aagggtaaca
     1981 tcatgtcgat cgatacgccg gaaaacatca tcagcacctt tccggatgca ctttatgctg
     2041 taaaagcaca tggtatgagc agactgcagg atgtgttacg caatgacccg ggagtcaaca
     2101 gttgctattc attcggcgat tatcatcaca tcacattcac agaggggaac aatgctgatg
     2161 accctgaaga gatcagggag gtacatgata aaacagaatt cgaccatttt cctgaatatc
     2221 ctcatgttag cgcccttctt cgacgcatac agcatgaggg atttcaggat ctggagataa
     2281 aaaggatcag tccaggtatc gaagattgct ttattcatct gatggaaaac aaattacatt
     2341 gaccaaatac tagccggaaa tggaaggaac aaagaccgga gtagcggaaa ataaattttt
     2401 gtccgcaagc gaagtggtga tccggacaga taaactcacc aaacgttttg gtgatttcgt
     2461 cgcaaccaat gaaatcacct tcgaagttta tgccggagag atcttcggtt ttctgggagc
     2521 caatggagcg ggaaaaacaa cggccatgaa gatgctttgc gggctttcga ggccttcatc
     2581 cgggaaggcc gtgatcgccg gatacgatat atataaagat actgaaagga taaagaagaa
     2641 gatcggctat atgagtcaga aattctccct gtatgaagac ttgacagtga tggaaaatat
     2701 tcggtttttc ggtggaatat acggtttaag taataagcat ttacagaaaa aaagtcatga
     2761 attgatagag acattgaatt tgcaaaatga agcgaatttg atggtgacat cgttgccttt
     2821 ggggtggaaa cagaaattgg cattttcagt ggctgtaatg cacgagccta gaatagtgtt
     2881 tttggatgag ccgacaggtg gagttgatcc tataacaagg cgacaattct gggatttgat
     2941 ctattccact gcggacaagg gcatcacaat ttttgttaca acccactata tggacgaagc
     3001 ggaatattgt gatcgggtgt cgatcatggt ggatggcgtg attgaggcat tggacactcc
     3061 ggccaatttg aagcgcaggt attcagtagc ttcgatggat gaggtgtttt accacttagc
     3121 gagaggagcc aaaagatcgg tagattgatg agaagcaaga tgagttaata atgggaaaca
     3181 ttcagtgaat ttggatcaaa agcgggaaaa atagggtcgt ttgccctaca aacaatagat
     3241 tgaacatgaa gagatttttc tcatttgtca ggaaagaatt ctatcacgtt tttcgtgatg
     3301 gaaagacgat gttgatgttg tttgggatgc ctgtgatgca gattctacta tttggttttg
     3361 cattgaccaa tgaaatcaag aattcacaaa tcgtcatctg cgactattcc cgggatgatg
     3421 caaccaggaa gatcatctct aaactcgaag cgagcaatat attccatatc cgggagtctc
     3481 tcctttccca tatgcagatt gaggaatcat ttaagtcgtc aaatgtaaaa ctggtggtaa
     3541 tcttcccgaa taattttaat catgacctgt tgcattctaa caaagctcag gtgcagatca
     3601 tagccgatgc ctccgatccc aacacagcta caacgctcac caattatgtt acaagcatca
     3661 taatgactta ccaacaacaa ttgatggcga tcaatgcttt tccgatgagt atcacaccgg
     3721 agatcaggat gtattacaat ccggaattga agggagccac caacttcgtg cccggtgtca
     3781 tagcattggt gctgatgttg atttgtgtga tgatgacttc agtgtccatt gtgcgcgaaa
     3841 aagaaaacgg aacgatggag atactgctgg tgtctccttt caatccgatg ctggtagtga
     3901 tctccaaggc cattccttac ttatttcttt ctattgtaaa tctcctgatc attctgattc
     3961 tgagtgtaag tctgctggat atgccgatca atggaagtat attcttactg atcgctgaaa
     4021 gcattctgtt tatcataacc gggttgtcat taggattgtt gatttccaat ttggtggaat
     4081 cgcaacaagc agcgatgttg atttcgatga tgggtatgat ggtgccgacc atcttattta
     4141 ccgggtttat gtttccaatt gagaacatgc ctattccgtt gcagatcatc agttatttca
     4201 ttccttcgaa atggttttat accatcgtca aggctatcat gataaaaggt cttgggtttt
     4261 atgacgtatg gaaggaaaca ctggtgctgg ccggtatggt tgtggtcctt ttgaccataa
     4321 gtttcaagaa atttaaaaca cgtttggcat gaggacattg aagtttttac tacaaaagga
     4381 attcaaacag atttttcgca acagagctat attgccattg atatttgtgg ttcctgttgt
     4441 acaacttctc atcctcccac tcgctgcgga ttacgaagtg aagaacatca atatttcagt
     4501 tgttgacggg gatcattcaa ccaattcccg gcaattgatt gcgaagatta cttcgtccgg
     4561 atatttcagg ctgaccgggt atggtacttc atttgacgag gcttacggac ttatggaaag
     4621 cgatgattcg gatttaatac ttgagattcc tgccggattt gaacgaaatt tagtccggga
     4681 gggagaacaa aaggttttcg tggcggtaaa tgccatcaac ggtgtaaaag caagtctggg
     4741 aggtgcctat ctcatgagga ttatatcgaa ctataacgat gacatcaggc ttgactggat
     4801 acagaagcca cggttcaatc catcgcctca gatcgaagtg acatcttcca attggtttaa
     4861 tcctcattta gattacaatt tcttcatggt gcctgccata ctcgtaattc tggttacaat
     4921 ggtgggttcg tacttgtgtg cgctaaatat tgttaaagaa aaggagatgg gtacaattga
     4981 gcaaattaac gtaacaccga tcagaaaata tcagttcgtt ttgggtaagc tcataccatt
     5041 ctgggtgatt gcgatgttcg tgtttacgct ggggttgttt gtcgtcgcct attgggtcta
     5101 tgggatcgtc cctctgggaa gttgggtggt gttgtatagt tacctggctg tctatctgat
     5161 tgcaatgttg gggttgggtt tgttgatttc tacattttcg gaaacacaac agcaggcgat
     5221 gtcgatctcg tttttcttca tgatgatatt tatgctgatg agtggtctgt ttacaccaat
     5281 cgatggtatg ccggaatggg cgtatgtcat tgcccgcagt aatccggtga cctattgtat
     5341 tgaagtggtg agaatggttg tattaaaggg cagtgggttt aatgatttga aacagcattt
     5401 cctgataacg gcagtttttg cagttgccct caacagcttt gcgatttgga actataagaa
     5461 gacgagttga ttgaatgata tatccggaat aattgacaat gcataaactt gtcaatatga
     5521 aaatatttaa tacctaatgt acattcgtaa tagaccaatt tcattggcct aaacgactgt
     5581 aacctcggca ttatacctga taattaagga ctattatata aatcaccttg gtataacatc
     5641 ggcatcccgg aaccccggat tgtttcactc ccgggacaga gcagagcttt agtagcacag
     5701 ctatccggac cagtatacct gtaactaccg gattatttta taattcatcc aggtataacc
     5761 tcgcgggaaa acaaatattg ataataaatg gatattttgg atatgtctgg taatgattca
     5821 atttttttat tgtgtgaatt tcaacggttg gtgctttaaa aaaagtgtat ggtattgtat
     5881 tgcctgttgg attttcggat actaaatatt taaatagaga aataacgaaa aggtcaaatt
     5941 aaattctttc atcctactat gctcaaaagt gatattattt gtattcaggc atatcaattc
     6001 agagatatcg actgaggcaa atattaatga tctgaatgaa tggatctgat ttgaataaag
     6061 caatcaaact ttcatgttaa cttttataac agggtatcaa aacaaggaaa ctttgttcaa
     6121 gaagaccgta caaacttcag tcagaatgcg agggtgctga gaattcggtt gctattaagg
     6181 ataaaataaa gcaatataaa gttttattta ttttggatta cctttttgtt ttattcagga
     6241 cgatcaattc atccttttgt catgtctcat cgtcctgcct tgtcattcct ttgaatgact
     6301 ttctatggtg aggggtaagg ccgtgtgtga aacaagcctg acggtgtttg gtggacagat
     6361 accctttgtt attgatccag tcatattgtg gaaattcgat gtgtagttct tccatatagg
     6421 catcccgcca ggtcttggcc aataccgatg ccgcagcaat gctaagaacc ttatcatcac
     6481 cacctacaac agttcggtgg ttgattccgg tttgattctt gaagcgattt ccatctatga
     6541 ccaacaggtc aggctttatc tttagttgtg caattgctct tgtcatggcg agataagtag
     6601 cttccagaat attgatttgg tctatctctt cgactgtaga aatggctatt gaataatcca
     6661 acgccaattc ccgaattttt ccacttaatt cctttcgttt agaagccgtc aattttttgg
     6721 aatcgcgtaa tccagggatt ttggcctttg gtggcagaat aacggctgca gccactacgg
     6781 gcccggcgcc gcatccgcga ccgacttcat ctacaccggc ttcgataccc gatccggctt
     6841 caaatggatg gatcagggga tctgccttct ctgcggctga tatttttatt tttcccaata
     6901 aatccttgtt ttgctaacat ccctgctgtt tgcaccgatc atgatgtcaa aatcgccgct
     6961 ttcccaatca aattgcaggt cataattgta aaatttcaaa tcttcaggag tgatttcaaa
     7021 gctcacggta actgtctggc gggctcctat ctccaccttt ttgaagcctt tcagctcttt
     7081 cacaggccgg gtgacgctgc cgacgacatc ccggatatag agctggacca cttccttggc
     7141 attgaaatca cttacattgg tgatttcaac ggaagcagtg agtttctgat tgcctttcag
     7201 gcttgttgat gaaaccttga tgtctgaata tctgaattta ctataactca atccaaaacc
     7261 aaacggatat agcggatcat tgctggcatc gaggtaattg gatctgaatt tctggaacca
     7321 gtggccttcc tggagtggcc tgcctgtgtt tttatggttg tagtagagtg gtatctggcc
     7381 tacattctga gggaaggtgg tggtcaactt gccacttgga ttaacatctc caaataatac
     7441 atcagctatg gcatcaccgg cttctgatcc tccaaaccaa acatctaaga tggccggcac
     7501 atgggcatct tcccaggtca gcgtcagggg gcgaccattg aataaaacga ggacgaccgg
     7561 tttgcctgtt ttgactaatt ccatgagcaa ttttttctga acgtccggaa tattgagatt
     7621 tgttcggctg ctgctttcac cactgaattc tgcggattct cccagtgcgg ctatgatgag
     7681 gtctgatgaa tgtgcggctt ccagggcttc ccgaagcaat tcttcatccg agcgattatc
     7741 ccggtctaag gatttgccaa acattgtgcc tctttcttct aatgcggcat cttcagttag
     7801 gttggaccct ttagcatagc gcagagtggc tttacctgct aaagcccttt caagaccctg
     7861 gcgcagggta attgatttgt cattgacggc tgctaccgac catgtaccgg tcatgttgct
     7921 ttgagcatga gccagtggac cgacgagggc gatggtaccg gattttctca gtggtagtaa
     7981 gtctccgtta tttttcaaga ggacgaaact ttctgcggcg atttttctgg cttcagcacg
     8041 atgttccggc gtgaaaattt cggttttgga tcgtttttca tcgcagaatt tataaggatc
     8101 ttcgaagagg ccgagtttgt attttgcttc aaggattagg cggcatgctg catcgatttt
     8161 agccatggtc actctacctt ccttgagtga ctttggcagg gtagtcaaca ggccttctcc
     8221 aaccatgtcc atatctatgc cggcattgag tgccattgca ctggctgtct gcaagtctcc
     8281 tatgccgtgg tcgatcatct cattgatacc ggtgtaatct gtcaccacaa atcccttgaa
     8341 cccccattgc ttgcgaagga catccgtcag taaccactta ttaccggtag caggtatacc
     8401 atccacttca ttgaacgaag ccatcacgct tcctacgccg gcaatgactg cagcctggta
     8461 aggaggaaaa tattcgttgt acatccggat gcggctcata tcagtggtat tatagtcccg
     8521 tcctccttct gcggcaccgt atagggcaaa atgcttgacg catgcgagga tctgattgtt
     8581 ttcggtcaag gttccctgat aacctcttac catagcctga gcaattgcac ttcccaggta
     8641 aggatcttca ccattgcctt ccgatattct tccccatcga ggatcgcgac acaggtctac
     8701 cataggagag aaggtccagt tgattccggc agcacttgcc tcgatggcag ctatccgtgc
     8761 acttctgcgg atggcatcca tatcccaact gcatgatagc cccagaggaa tgggaaacac
     8821 cgtctcataa ccgtgaataa catccatacc gaacaacagt ggtatcttca accggctttc
     8881 ttccacagcg attcgctgaa cctctcggat tttctctact gatttgatat tgaacaggcc
     8941 acctacgttg ccctccctga tttttttacc gatatccgag ctggaggcct ggcctgtagt
     9001 gatatcgcct gcgacaggca agttcaactg acccagtttt tcagtcaatg tcattttcga
     9061 catcaattca tcaataaaga ccttcatcct gtcgggcttg cctttctggc caaaggaaac
     9121 ttgagagatg agcaaaaagc aaagtagata cagactgcgc aatactgtca tggtttgaat
     9181 tttttaagga taaatatagg agatttcaca tggagaggtg gaaatatttt tgaaagtata
     9241 actgatcatt gatgtaaaga tattgctcat caacttacta ttactatttg atttagggaa
     9301 atgtgtactt tcatatccct gatttttggt ccctttttaa tagattcatt tatgtcaagc
     9361 tgaaagtcaa aaggagatgc ccttgttttt ttaatttgat aaagggacat tatttaatgt
     9421 acggtaatgt caataattac ctgacggatt gatagtccga agatttgggg aggggtaggg
     9481 aaatgctgga tggaaattta taatcaggat tgacaactat ttattcaaat tcccttaagc
     9541 ctgttatttt tgttgtgaaa tcaagaaagt aacatgtctg tttcaaagaa attcttttgt
     9601 gtggacgccc atacctgtgg taatccggtt cgcctggttg caggtggagg gcctgcattg
     9661 atcggggcca atatgagtga aaaaaggcag catttcctta gcgagtatga ttggattaga
     9721 aaaggactaa tgtttgagcc gcggggtcat gatatgatga gcgggagtat cctttatccg
     9781 ccgcatgatc cggcaaatga tgtcgcagta ctttttattg agacaagcgg ttgcttgcct
     9841 atgtgtggcc atggcaccat agggactatc actattgcca tagaagaggg tttaataagg
     9901 cccagggaag ccggaaaggt caggatggag acacctgccg gtctggtatt cgtgacttat
     9961 catcaagaag gtaaaaaggt caaaagtgtc aagttgataa atgtgcctgc ttatctagat
    10021 tcggaagggt tggaagcaga atgcccggat ctgggtatgt tgagagttga tgttgcatat
    10081 ggcggaaatt tttatgctat cgtcgatgtt caagaaaact ttgccgggat cgagcattat
    10141 actgcagaca agctggtagc ctgggccagg gcattacggc gcaatatcaa tgagagatac
    10201 agttttgttc atcctgaaga tgatactatc cgtggttgct cacatatcct gtggacagga
    10261 aaaatcctcg acccaaactc tacagctcgc aatgcagtgt tttacgggga taaagcgatc
    10321 gacagaagtc cgtgcggaac cgggacttcg gctcgaatgg cacaatggta taccaagggc
    10381 ttactgaaac ccggtgatcg ttttgtacac gagagcatca tagggtcgaa atttgttggt
    10441 acgatagagg aggtaacaac ggttgcagga aggcctgcca tacggcccgg aatcgaaggt
    10501 tgggcacgta tatacggcta caacaccatc agtattgatg acgaggatga tccatttgct
    10561 tttggatttc aagtaattta accaattgtt ataacgaatt aggtcacctg ttttaaatag
    10621 attgcggaaa tgaaagcagt aattataggt gggggcatta tcgggttgag ctcagcattt
    10681 ttcctgaaaa gcagtggatg ggatgtcata gttgtagatc agggcgacat gacgcacaat
    10741 tgttcgtatg taaatcttgg gatggtcgtc ccgagtcatt ttgtgccatt ggcagctccg
    10801 ggcatggtgt cgcaaggcat tcgatggatg tttgaccgga agagtccttt ttacgtcagg
    10861 ccgactctga gtgggagatt gatttcatgg gggttaaaat ttatgagaag tgccaatgat
    10921 agtcatgtga gtaatgctgc tgaacctctg agagatctga gtcttctgag tcgcagtatt
    10981 ttcgacacat gggccgcaat gccggaatta gacttcggat atgagcgcaa aggcataatt
    11041 atgtatttca attcttcaaa aaccggtgat gaagaagtgc atttggctga aaaagctgtc
    11101 aaacttggtt tggatgcgat tgttctaggg ccgcaggagc ttagtcgact tgagcctgca
    11161 gtccagccta atgtcatggg gggcgtacat tatcgttgtg atggtcatct gaatccggac
    11221 aggctcatga ggcagttgat ggggctattg gccgataaag gggtattgat cgaaagaaat
    11281 agggaagtaa taggttttac tcaaaatcac gggaggatca cttcggtaaa gaccaaagaa
    11341 ggggcgatgg atacggatct ggttgtcttg gccaatggtt catggttgcc tgacctggct
    11401 aaaatggtag ggatgaatat tcctttgatg ccgggcaaag gttattcctt catgcaagca
    11461 aacccgccgg cgcagctgaa tatacctgct attctttgtg aggccagagt agccattaca
    11521 ccgatgggag ggggaattcg ttttggtggg acaatggaaa ttgaccggat tagagagggt
    11581 ggtgtcaaca tgaaccgtgt tgcagggatt gtggaatcct tgccgcgata ctttgataac
    11641 ttccgagttg aaatgcctga tatgcatcag gtttcatatg gatacaggcc ttgttctccg
    11701 gatgggatgc cttacatcgg ttcatctcgc aggcatgaca acttgcttgt tgcaggtggc
    11761 catgccatga tggggttgag tcttgggcct gtaacgggaa agctgattgc tggtttggcc
    11821 aatggagaaa agcccgaaat agatttgaag ccttttgatc caaacagatt tgattgagta
    11881 aaaatagcaa tattggtaat ttagcaggga gtggctggtt ggtaaaaggc aggtgagatt
    11941 ggacctggag aggattggta aggggggtgt tttgaatgaa gtatgtagat gtttaatagc
    12001 gttgaatcaa gtgttgataa ttcattccgg gctttgttaa taatattttg caagatttca
    12061 ataaatttat tcttgacaat tttttgaaac ctgtatgctg atgaggtatg aggttattaa
    12121 acaagtacat acttcttttt ttttcaaggt gagggaggtg tcaccctgaa gtaggtagat
    12181 gtttactagg tttagatttc gtgttggtgt gatttttcta acctcgtcct tctttttttt
    12241 cttttttttc aattgtttta tttttattca taagcagaaa taagcgcact ggttgagtat
    12301 gaggttatta aacaagtgca tacttcaatt tttccccacc caccccttgg tcatcccaaa
    12361 tctttttact acct
//
LOCUS       DAOQQN010000037        12347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1790_length_12347_cov_6.334852, whole genome shotgun sequence.
ACCESSION   DAOQQN010000037 DAOQQN010000000
VERSION     DAOQQN010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12347
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1790_length_12347_cov_6.334852"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="PLG24_02395"
     CDS             <1..1107
                     /locus_tag="PLG24_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ23763.1"
                     /translation="TLIRIAGQSKLLERDFTKQVGTGPYKVSEWVPNQRLTLVRIEQY
                     WGMPLAETYTEFQAFPATINYLIFPEVSTAMAALKAGQVNVMPEVNEEILDQVRSDAT
                     LAKNFDVLSADVLQYYYIGLNNAGPMLGDLNVRRAMNHLVNVDRIIQTLMHGNAEKVK
                     SPILAQKPYYVSNLPDIEYNPAKARAILDQFGYHEIGADGIRTKKINGQPMRMSFTIL
                     TTGKQLGRDVATLLINEARKVGIEVKMETMDFPKILERIKSGKYDMANLVVKQFPGLD
                     DPYLAWNSTNAFGKGSNYCNVNNPAIDRITEEIRTAPSQADRYKLYREFQVKLAEQYP
                     VIFLFSPKNQILIQEGLTMVTSPKRPGYFENTVK"
     gene            1138..2439
                     /locus_tag="PLG24_02400"
     CDS             1138..2439
                     /locus_tag="PLG24_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ23764.1"
                     /translation="MTTGIIYNLLRIFAGLLKLMIKVLVIVLSVYFLASIIGSRNIED
                     GDTYQGDEFKTHLPAFYFSVLPGWYDARLSLYPAGPVKKQGIDFLKNGLTYNDWLKFR
                     ALVDTISGRPQYKNWNELKYIHLISEIDSRIRKESKNAKPDEFLQKNLAEISGLLTGR
                     HRITGLKYLPQLQFWGKSNNFHQELMSVFSTTDKWLQLRSAFNLSFVLSLLAYLSTIL
                     FGTLLALWYMRMKSLGRRILMTYLNIIYVFPVFCLTVIAVQFFTSDYYSPWLNWFPGP
                     GTFLMMSSVSLGSVFSDSVGYFILPALLLSLPLSAGAAMRWITGMREEMDKPYVITLK
                     SKGISNHDINTVHVLKNVSLPMIVYFGMLFPSLMSGVLLIENVFSIGGIGRLTWQSVR
                     YDDFMMLLVITALVAMINIFMSQLASKIMKYVDPRLKVAGS"
     gene            2436..3542
                     /locus_tag="PLG24_02405"
     CDS             2436..3542
                     /locus_tag="PLG24_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMZ23765.1"
                     /translation="MKQGVRHIFQKFKWWLVLPLAYLLLKCLINPEPVVSIDSSGVRF
                     GLFNFYNISVKMQPAADLSIKPLLYISAYETNIDGTTSLPPWTRSEDGKLHIMGTDHL
                     GRDVFAGFVAGLEIALILGFAVSLASGLLSLLMGGAGSYFGRFPIKVSWVELSIWWIG
                     GFIFLIITSWWIIGAVSGWSWLTVIILVFAFIILARRFFQRRKHILPVSSFNRRIQEF
                     FQPLPDLVILLVVASIFDSIGFTGLIIILISLRLPTGAWYLQGQAQQFVYLPHIDQAK
                     VMGIQDSRIIVRHLAPMMLPATGTFMSLTAARAVLAESALSFLGIGPTGELMSWGSMI
                     RLGLSNMQIWWVALFPVLGLVGLTMLFRIKTYSK"
     gene            3539..4087
                     /locus_tag="PLG24_02410"
     CDS             3539..4087
                     /locus_tag="PLG24_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23766.1"
                     /translation="MRKKWTYRNRRIFILGLTIFLCLFFRSVEGQTQVVPPKTFAVGG
                     DLALFTTLGAGDRTAAIYFSPSLEYYVVQNGALAMKFVLDFSNITENSLVKIVTKRIF
                     QPYLRYHFLNGCTFLAGTQYDLEFKYQPNLHWGAGYSFFVMPRVCITPLMEFNHNFYK
                     FAARPFRMNFSFGAAYYFNRKV"
     gene            4084..4377
                     /locus_tag="PLG24_02415"
     CDS             4084..4377
                     /locus_tag="PLG24_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23767.1"
                     /translation="MRIALFVLLNLIAYMAFGQQVRKQYFLVGPRGQFSGYTIKGQGG
                     FYYDYNFSMDVEYSFLQNTSGGIRLGMDGSKSSVIKNTIYVIGPFSVNICGRD"
     gene            4374..4574
                     /locus_tag="PLG24_02420"
     CDS             4374..4574
                     /locus_tag="PLG24_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23768.1"
                     /translation="MNVQASYVFRGKDRPRFGLGMGYGFETGRRTIFQPEVEYLYYFS
                     KGAGDASLRFGLSMRFILGKAN"
     gene            complement(5206..9135)
                     /locus_tag="PLG24_02425"
     CDS             complement(5206..9135)
                     /locus_tag="PLG24_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23769.1"
                     /translation="MARVYQSKKVIGYQRPNIEPHEEDTLHEPKGMSFPAVPFNFTAQ
                     SNTYLFQNNLPKNQSTNPCLTKIFQEHKYIETNLAKHKALIDEGQKKVIDIWLLNYTK
                     LETLEYSELEFYKNDEIEKYNKLIISCPEDEKDPQYLNYLRVVDVLKDKIKIYDSIQA
                     ERDKNDQQAEKDVDNIYNYGQTFNIDQLLRTLQSSFPRREKTREIFFKKYGERLDVFV
                     KFHTSIFSSEGFYWSMAQAYLEVGKLRPADKFYIAVKGKGTDEGNVYFAMREAHAYGV
                     DKLENDIRNEYPGFESKGSGLLHTGWGNDIWIAAQWIDSETSSDSDERIKMKALLSFG
                     QVRPLDEIRMALKGDTSKGDLINALRKANAENLIEVREKNNDKCLADSAEKKMVSQKL
                     LDDYKFSYGGDLMTDIQAIYTTYQSNHDGDEIVDSTISKVVDEKGLAIVNKIISGEET
                     KDEQWLNLMKSGKRKEAFNFYLHSSTDDQSIILSYIFPILVENTKTEFQFTDIEKTQY
                     ERALDNDNQEGFSFLKNLGLLNDSKKILNYFKYSANRMEIEIFNNYYNNLKDFRFQIN
                     MLLGNSNDLLLHRILAVNQPFYQLLEALRVDENWANHLMLHIYTEEEKKAILNDKRFN
                     ELGLESDVTRYIDYFMNGMSPEKVAEKHADKEKLKKVTGLLTNDITFRVKAERSLLEK
                     ANSGLGASITNTFSTSKLSAENEQRELEVSLEMALWNDEKIDEKEMMGIEKDILDVRE
                     TRGNFEEIRNTVESVATIIAQAVVQIAATALIPGAGGILAQAIIGGISSTGSNWVMKG
                     DRYSLNNGLKDMAIGGLNGAMGALGNIKVAQWSSGEGSFSSILKQSKLLQETVENSIG
                     NSHELIIDAVEGKSPDEIFKNHLKSVGIGIVTNRITQLIESKIKRDKDSILEDGQSNL
                     DTESISAQIGEGGITQSSYDVKHPKDWTDQDFIEYLKGKKNWDEAIDELKMVGGDELA
                     FRLHKIRAKLQYELIGEGAAPLSDASNDVKSDVDLNIEGDNAGKILLKLEKELAEKYG
                     LTIDEVKKMFKLEFFTDAPSRLGRYQQLQGKVSRDRYEEIVSDITFHQETYQYAKMLR
                     EAQGEHAFRIETIIHGRPDESIIRKLATLSPSEIKAQREKLLKEIDKLVARSNSGKLK
                     PKDREKIDIQISKKQAELNFYSNDAYISFGAMTKKPQSSLEMKGSMLSQLQMIQSKIA
                     TYGAGEAAKGYEVYKYIKRYLEYLEASGIALSPKMLHLKRLSNEIYPSGNFSRTFYES
                     YAVNRSNSERHYMLVSFWEEAHNIVNNTDWENLNFDPAKAAASLTTP"
     gene            9564..10376
                     /gene="kdsA"
                     /locus_tag="PLG24_02430"
     CDS             9564..10376
                     /gene="kdsA"
                     /locus_tag="PLG24_02430"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HMZ23770.1"
                     /translation="MNTLELIQSRSNRQFFLIAGPCAVESKDLCFEVAETVNRLTSER
                     DIPYVFKASFKKANRSKDNSFTGIGEEAGLEILGEVRKYFGLPVTTDIHESYHAELAA
                     KYVDVMQIPAFLCRQTDLLKAAGHTGKTVSIKKGQFLSPEGMRFAVDKVLHTGNDKVW
                     LIERGTTFGYHDLVVDYRGIPTMRSFEVPVVMDCTHSLQQPNQASGVTGGNPSMIGTI
                     AKAAIAVGADGLFIETHPDPAVALSDGANMLKLDRLEALLDQLVKIRRAIID"
     gene            10379..11956
                     /locus_tag="PLG24_02435"
     CDS             10379..11956
                     /locus_tag="PLG24_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ23771.1"
                     /translation="MRSHQPEFYEIKVAPADLDDIRVHESLFRSNYGHLKVPFDSLIL
                     TRRSIDARKGKVVYVLRYQKVSDVPADNGFHDLTPLADPEHCPQVVVVGAGPAGYFAA
                     FRLIQSGIRPVVVERGKDVQSRRRDLRAIMQESIVNEDSNYCFGEGGAGTYSDGKLYT
                     RSTKRGDVRQILNTFCFYGADPRILIDANPHIGTNKLPKIITAMREAILKAGGRILFE
                     HRMVDFDIEDGRLAKVHFRHQPSIECKAMILATGHSARDIYRLLANRGLKIEAKPFAM
                     GVRVEHPQSLIDSIQYSCPTRPEGLPAAPYSVVSQEGGRGVFSFCMCPGGMIVPAATS
                     PGELVVNGMSPSRRDSPYANSGFVTSVELEDIPAEYGEDAFVAMNFQSSVEQRLFNYG
                     NGTQSSPAQRLVDFTRKKESTDLPNCSYVPGIYPAPMHRILPEFIVDRLISGFLSVGK
                     KLHGYLTNEAVVVGVESRTSSPIRIPRDKESLRHPDLVNLFPCGEGAGYAGGIVSAGI
                     DGVNVAVAVSKYLTMEG"
     gene            complement(12027..>12347)
                     /locus_tag="PLG24_02440"
     CDS             complement(12027..>12347)
                     /locus_tag="PLG24_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23772.1"
                     /translation="VHAHTFVSENQKDIDNYFHYYSRQMNRIGQSRGWPTFTHGQYEA
                     GRSKHGAYLFGSSQEVSEKILMMKEVFGLTRFIGHIDVGAPPHDMLMNTIELMGTKVV
                     DKVK"
BASE COUNT         3400 a         2430 c         2485 g         4032 t
ORIGIN      
        1 accttaatcc gtattgccgg acagtctaaa ttgctcgaaa gggactttac caagcaagtt
       61 gggacgggtc cgtataaggt cagtgaatgg gtgcctaatc agcgcctcac tttggttaga
      121 atcgagcagt actgggggat gccactcgcc gaaacctata cggagtttca ggcgtttccg
      181 gctactatta attacctcat ctttccggaa gtatcgacag caatggctgc actcaaagca
      241 ggacaagtca atgtaatgcc tgaggttaat gaggagatcc tggatcaggt tcggagtgac
      301 gctacactgg ctaaaaattt tgacgtactc tcagccgatg tattgcaata ctattacatc
      361 gggctgaaca atgcgggtcc tatgcttggc gatcttaatg taagaagggc aatgaatcac
      421 cttgtcaatg tggatcggat tattcaaacc ctcatgcatg gtaatgctga aaaggttaaa
      481 agtcccatcc tggctcaaaa accttattat gtgtccaatc tgccggatat cgagtataat
      541 cccgcaaagg ccagagctat tctggatcaa tttggatatc atgaaatagg agctgacggc
      601 atccggacaa agaaaatcaa tggccagcct atgagaatgt catttaccat tctaacaacg
      661 ggaaaacagt tgggtagaga tgtagcaacc ctgcttatca acgaggccag gaaggtcgga
      721 atagaagtaa agatggaaac catggacttt ccaaaaattc tggaacgaat caaaagcggg
      781 aagtatgata tggccaatct tgtggtaaaa cagttccccg ggctcgacga tccatatttg
      841 gcatggaatt ctaccaatgc ttttggcaaa ggctccaatt attgcaatgt gaataatccg
      901 gccatcgatc gcattacgga agaaatcaga acagctccct cccaggctga cagatacaag
      961 ctctataggg aatttcaggt aaaactggct gaacaatatc cggttatctt cctttttagc
     1021 cctaagaatc aaattcttat ccaggaaggt ttgaccatgg taacatcacc caagcgacca
     1081 ggctattttg aaaacacggt taaataggct tttttgaaga aataaaatca taatgatttg
     1141 acaaccggta tcatatataa tcttctcagg atttttgccg ggttgttgaa gttgatgatc
     1201 aaggttctgg ttattgtatt gtcggtatat tttctggcga gtatcatcgg cagccgaaac
     1261 attgaagatg gtgatactta tcagggggat gaatttaaga cacaccttcc ggcattctat
     1321 ttttcagtat tgcccggatg gtatgatgct cgtttgagtc tttatcccgc aggtccagtg
     1381 aaaaaacagg gaattgattt tttgaaaaat ggccttacct ataacgattg gttgaaattt
     1441 cgtgccctgg tcgatactat atcaggacgt cctcaatata aaaactggaa tgagctgaaa
     1501 tatatacacc tcatttccga aatagattca cgcatccgca aggaaagtaa aaatgcgaaa
     1561 ccggacgaat ttcttcaaaa aaatctggcg gaaatatccg gattattaac cggcagacat
     1621 cgtattacag gtttaaaata tttaccacaa ctacaattct gggggaaaag taataatttt
     1681 catcaggagt tgatgtcagt tttttccacc accgacaaat ggcttcaatt gcgatcagcc
     1741 tttaatcttt catttgtttt gagtcttctc gcctatttgt ccaccatttt attcgggacc
     1801 ctactggcac tctggtatat gcgtatgaag tccttgggcc ggagaattct aatgacctat
     1861 ctcaatatca tctatgtttt tccggtgttt tgcctcacag taatcgctgt acagtttttt
     1921 acttcagatt attatagtcc atggctgaat tggtttcccg gcccggggac cttcctcatg
     1981 atgagttcgg tgtctttggg aagtgttttt tcagactcgg tgggctattt tatacttccg
     2041 gccctattgc tgtcgttgcc cttgagtgcg ggtgccgcta tgaggtggat taccggtatg
     2101 cgggaagaaa tggacaagcc gtatgtaatc actctgaaaa gtaagggcat aagtaaccat
     2161 gatatcaata ctgtgcatgt cctaaaaaac gtatccctcc cgatgattgt atattttggt
     2221 atgttattcc cgtctttgat gagtggtgtg cttttaatag aaaatgtatt ttcgattgga
     2281 gggattggaa ggctgacgtg gcaaagtgtt cggtatgatg atttcatgat gttactggtc
     2341 ataacagcat tggtggccat gattaatatc tttatgagtc aattggcaag caagatcatg
     2401 aaatatgtag acccaagatt gaaagtagca ggttcatgaa gcaaggggta agacatattt
     2461 tccaaaagtt caaatggtgg ttagtccttc cattagccta tctcctgcta aagtgtctta
     2521 tcaatcctga gcctgttgtc agcatcgatt ccagtggtgt caggttcgga ttgttcaact
     2581 tttataatat ttcggttaag atgcaaccag cagcggattt gagtataaag cccttgttgt
     2641 atatatccgc ctatgagacg aatatcgacg gtacaacctc tctgccaccc tggaccagaa
     2701 gtgaagatgg taagttacat atcatgggaa cggatcatct gggccgggat gtatttgcgg
     2761 gttttgttgc cgggcttgaa atagccttga ttttgggttt tgctgtttct ctggcttcag
     2821 gcctgctcag tctattaatg ggtggtgccg ggtcgtattt tggtcgtttc ccgataaagg
     2881 tttcatgggt agagttgtca atctggtgga ttggcggttt tatatttttg attattacat
     2941 cctggtggat tatcggagca gtttcgggat ggagttggct gacggttatc attttggtat
     3001 ttgctttcat cattttggcg cgaagatttt tccaacgcag aaaacacatc ctacctgttt
     3061 caagcttcaa ccgacggatc caggaattct tccagccatt gccggatctg gtgatcttgc
     3121 tggtggtggc ttcaatattt gattcaatag gcttcacggg attgataata attctgattt
     3181 cactgcgttt gcctacaggg gcatggtatc tgcagggtca ggcgcaacaa ttcgtgtacc
     3241 tgccgcatat cgatcaggca aaggtgatgg gaattcaaga tagccgtatc attgtccggc
     3301 atcttgcacc aatgatgttg cccgccacag gcacattcat gtcgttgact gctgcccgtg
     3361 cagtattggc agaaagtgca ctatcctttt tgggaatagg tcctaccggt gagctaatga
     3421 gctggggttc gatgatcagg ctgggcttgt ccaatatgca aatttggtgg gtcgctctgt
     3481 ttccggtttt ggggctggtg ggattgacga tgttgttcag aattaaaacg tacagcaaat
     3541 gagaaagaaa tggacttata gaaatagaag aatatttata ctgggattga cgatcttcct
     3601 ttgcttattt ttccgatcag tggaaggaca gacccaggtg gtacccccaa aaacttttgc
     3661 tgtaggaggt gatttggcat tattcaccac attaggggca ggggaccgca ctgcagcaat
     3721 atatttttcg ccatcactgg aatattatgt tgtacaaaat ggtgctttgg caatgaagtt
     3781 tgtccttgat ttttccaaca ttacagaaaa ttcactcgtg aagatagtta ctaagcgaat
     3841 cttccagcca tatctacgtt atcattttct caatggatgt acctttctgg ccggcaccca
     3901 atacgatctc gagtttaagt atcaacctaa cttgcattgg ggtgcagggt attcattttt
     3961 tgtaatgcct cgtgtatgca tcacgccact gatggaattc aatcataatt tttacaaatt
     4021 tgccgcaagg cccttcagaa tgaatttttc ctttggggca gcctattatt tcaaccggaa
     4081 agtatgagga tcgcattgtt tgtattgtta aatcttatag cgtatatggc gtttggtcag
     4141 caggtgcgta agcagtattt tcttgttggg ccgcgaggtc agttcagtgg atatacaata
     4201 aaggggcagg gtggttttta ttacgattat aacttttcga tggatgttga atattcattt
     4261 ttgcaaaaca catccggggg catcagactg ggcatggacg ggtcaaaatc ttctgtcatt
     4321 aaaaacacga tttatgtgat cggccctttt tccgtcaata tttgtggaag ggattgaatg
     4381 tacaggcaag ttatgtgttc agaggcaaag accggccgag gtttggcttg ggcatgggat
     4441 atggttttga gacagggagg aggacgattt tccagccgga ggtagaatat ttgtattatt
     4501 tttcaaaagg ggcaggtgat gcttcgcttc gtttcggatt gtcgatgaga tttattttgg
     4561 ggaaagcgaa ttagcaggaa aggggggcgg accctgaatt aggtagatgt ttaatagacc
     4621 agaatatagt agcgatcatc gttcctggca aatgatattt tagaactagt ctttttttca
     4681 atgtcttttt ccaatcaatt tctgaagcac tctacatcat taggttttca gttattaaac
     4741 aagtacatac ttggaatttg gattcaggaa ccatttatct acttcattgg aacaatcgac
     4801 cgatactgtt taaaaaagtg tgtaaagcca aaaattaaga tttttgtaga taacaaaaca
     4861 aaatcagaaa aaaattgact tcacacaaga acaaatttcg gagttattat tgacaataac
     4921 aaacgaaaaa gatggttatc gaattttaaa cagtatcaac ttcagaaaga tccaatatat
     4981 ttttgaaatc aatgatatga ggcttcataa gttcgattcc gggatatcca caaactcctc
     5041 aatcatcact cctatgtggc cccctttgct ccggaatcag tggccccctt tactccggaa
     5101 tactcaggtt gattaggaca ccaacagcga ctactttact agcagcatga ggtgtggcca
     5161 ctgccgaaga attgtcaaaa gagcaaagcc ctccatcata ccattctacg gtgttgtcaa
     5221 acttgctgct gctttagcag gatcaaaatt caggttttcc caatccgtat tgttaactat
     5281 attatgggct tcctcccaaa aggaaactaa catataatga cgttctgaat tacttcgatt
     5341 cactgcataa gactcataaa aagttctact aaaattgccg gatggataga tttcgtttga
     5401 aagtcttttc aaatgcagca tttttgggct taaagcaatt ccacttgctt ccaaatattc
     5461 taaatatctt ttaatgtatt tataaacttc ataccctttt gctgcttcac cggctccata
     5521 tgttgcaatc ttcgattgaa tcatctgaag ctgactcaac atagagcctt tcatttcgag
     5581 agaagactgc ggctttttgg tcatggctcc aaaactgata taagcatcat tggaataaaa
     5641 gtttagctct gcttgctttt tacttatttg aatatctatt ttttctctat cttttggttt
     5701 taactttcca ctattggagc gtgccactaa tttatctatt tcttttaata atttttctcg
     5761 ctgagccttt atttcagatg gtgataaagt tgctaacttt cttattatgg actcatcggg
     5821 tctcccatgt ataattgttt ctattctaaa tgcatgttcg ccttgggctt ctcttaacat
     5881 cttagcatat tgataggttt cttgatgaaa tgtgatgtca gatactattt cttcatacct
     5941 atctctactt acttttccct gcaactgctg gtatctacct aaacgacttg gcgcatcggt
     6001 aaaaaattca agcttaaaca ttttcttgac ttcatctata gtaagtccat atttttctgc
     6061 taattctttt tctaatttta agagaatttt tcctgcatta tccccctcaa tattcaaatc
     6121 aacatcactt tttacatcat tggatgcatc agaaagtggt gccgcccctt cacctatcaa
     6181 ttcgtattgt aacttggctc ttattttatg aagtctgaaa gccaactcat ctcctccaac
     6241 catctttaat tcatcaattg cttcgtccca attctttttc cctttaagat attctataaa
     6301 atcttgatct gtccaatctt ttgggtgttt tacatcatat gatgattgtg taataccacc
     6361 ttctcctatc tgagcactga ttgattctgt atctaaatta ctttgcccgt cttcaagtat
     6421 cgaatcttta tctctcttaa tctttgattc aattagttgt gttattctat ttgtgacaat
     6481 cccgattcct actgatttta aatgattttt gaatatctca tccggacttt taccctcaac
     6541 tgcatcaata attagttcat gactatttcc aattgagttt tcaacagtct cctgcaatag
     6601 ttttgattgc tttagtattg aagaaaaaga accctcacca cttgaccact gagctacctt
     6661 aatatttcct aaagccccca ttgctccatt taagccacct atagccatat ctttcaaccc
     6721 attgttaagg ctataacgat cccctttcat gacccagtta gaaccagtac tggatattcc
     6781 ccctataatg gcttgagcta atatgcctcc tgccccaggt atcaaagcag tagcagctat
     6841 ctggacaaca gcttgggcta ttatagtagc tactgattca acagtatttc gtatttcttc
     6901 aaaatttcct cttgtttctc tgacatctag aatatctttt tctattccca tcatttcttt
     6961 ttcatctatc ttctcatcat tccataatgc catttctaaa ctcacttcca attctcgttg
     7021 ttcgttttca gcacttagtt ttgaagtact aaatgtattg gtaattgaag cacctaaccc
     7081 agaatttgct ttttctaata gacttctttc ggcttttact ctaaatgtta tatcatttgt
     7141 tagaagtcca gtaacctttt tcaacttctc tttatcagcg tgtttttcgg cgaccttttc
     7201 aggtgacata ccattcatga aataatctat ataacgggtg acatcacttt caagccccaa
     7261 ttcattaaat cttttatcat ttaatattgc ttttttctct tcttctgtgt aaatatgcag
     7321 cattagatga tttgcccaat tttcatctac tcttaaagct tctaatagtt gataaaatgg
     7381 ttgatttaca gctaatattc tgtgcaacaa caaatcgttg ctatttccta aaagcatatt
     7441 tatctgaaat ctaaaatctt ttaagttgtt atagtaatta ttaaaaattt ctatttccat
     7501 tctgttagca gaatatttga aatagttcaa aatcttttta ctatcattta ataaacctaa
     7561 gtttttaaga aaagaaaatc cttcttgatt atcattgtct aatgccctct catactgtgt
     7621 tttttcaata tcagtaaact ggaactctgt ttttgtattt tcaactaaaa taggaaagat
     7681 atatgatagg atgatacttt ggtcatctgt acttgaatgg agataaaaat taaaagcttc
     7741 ttttctcttc ccacttttca tgagatttaa ccattgctcg tccttggttt cttctcctga
     7801 aattatttta ttaactattg ccaatccttt ttcatcaact acttttgata tagtagaatc
     7861 aacaatttca tctccatcat gattgctttg ataggttgta taaattgcct gaatatctgt
     7921 catcaaatca cctccgtagc taaacttata gtcatctaat agtttttggc ttaccatttt
     7981 cttttctgct gaatcagcca aacatttgtc attatttttc tctcttactt ctattaaatt
     8041 ttctgcattt gcttttctta gtgcattgat taaatcccct ttactagtat ctccttttaa
     8101 agccattctt atttcatcaa gaggtcttac ttgaccaaag ctcaataagg ctttcatttt
     8161 aattcgctcg tccgaatcac tagatgtttc gctgtcaatc cattgtgctg ctatccaaat
     8221 atcatttccc catccggtat gaagaagccc tgaacctttg ctttcaaaac ctggatattc
     8281 atttctaata tcgttttcca atttatcaac accataagca tgggcttccc tcatagcaaa
     8341 gtaaacattg ccttcatccg tccctttgcc ttttacggct atataaaatt tatctgctgg
     8401 acgtagcttc cctacttcta aatatgcttg agccattgac cagtaaaatc cttcgctact
     8461 aaatattgaa gtatgaaatt taacaaagac atctaacctt tcaccatatt ttttgaaaaa
     8521 tatttctcta gttttttctc ttcttggaaa tgaggattgt agggttctta ataattgatc
     8581 tatattgaaa gtttgtccat agttatatat attatcaacg tctttttctg cttgttggtc
     8641 atttttatct ctttcagcct gaattgaatc atatatttta attttatcct ttaagacatc
     8701 aacaacacgt aaataattta aatattgagg atccttttca tcctcagggc aactgattat
     8761 aagcttgttg tatttttcta tttcatcatt tttataaaat tctaattcag aatattctaa
     8821 tgtttctagt ttagtataat taagtaacca aatatcaatt accttttttt gcccttcatc
     8881 tattaatgct ttatgttttg caagatttgt ttctatatat ttatgttcct gaaatatttt
     8941 agttaaacaa ggatttgtac tttgattttt tggtaaatta ttttgaaata aatatgtatt
     9001 gctttgagca gtaaaattaa aaggcacagc aggaaagctc attcctttag gttcatggag
     9061 cgtatcttcc tcatgcggtt ctatattcgg acgttgatag cctataacct ttttagattg
     9121 atatactcgt gccatttttg aggttgttaa taggtgaaaa accaaagtca ataaaatatt
     9181 ggtcaattac atttgtagtc ataattcact tcatttggtt tagtaaattt attattaatt
     9241 ctgctatttt tcaataacag tataatccta ttgttaaaat ttataaaatc agatcgaaaa
     9301 taaaatcgaa gcaactactt gacatacaaa atcttgcttt tctatttgta cctactggca
     9361 ttatccataa tagctttttg tgtactggaa ttcaaaattg ttccgtttgt tgtatatgaa
     9421 aattgggtat tagattctga cttaaccttt tgattggttt cgatccaaac atcacttgac
     9481 tcatccgatg gaatttctac cagagcaata atatcatttt catttgccga tattggccta
     9541 cttttgcaca aaaatcagga gcaatgaata cgctggaatt aatccaatca agatccaatc
     9601 gtcaattttt tctaatagcg ggaccttgtg ccgttgagag taaagactta tgctttgaag
     9661 tagcggaaac agtcaacagg ctaacatctg aacgggacat tccctatgtt ttcaaggcat
     9721 ctttcaagaa ggcaaacagg tcgaaggaca atagttttac cggcattggt gaagaagcag
     9781 gtcttgaaat cctgggagaa gttagaaagt attttggtct tccggttacg actgatatac
     9841 acgagtcata tcatgcggag cttgccgcta aatatgttga cgtcatgcaa atacctgcct
     9901 ttctctgcag gcagacggac ttgttgaagg cggctggtca taccggtaaa acagtgagta
     9961 tcaagaaggg acagtttctg agtcccgagg gcatgcgctt tgctgtagac aaggtgcttc
    10021 atacaggaaa tgacaaggtt tggttaattg agcgcggtac tacatttggg tatcatgatt
    10081 tggtagtgga ttatcgaggg attcccacaa tgcgtagttt tgaggtgccg gttgtgatgg
    10141 attgtacgca ctccctccaa cagcccaacc aggcttccgg cgttacagga gggaaccctt
    10201 ccatgatagg taccattgct aaggcagcca ttgcagttgg cgccgacggt ttatttatcg
    10261 agacccatcc cgatccggct gtggcacttt ctgatggtgc caatatgttg aaactggatc
    10321 ggttggaagc attgctggat caattggtaa aaatacgaag agccatcatc gactgattat
    10381 gcgcagccat caacctgagt tttatgaaat aaaagtcgcg ccagctgacc ttgatgacat
    10441 cagagtgcac gaatctttat tccggtcaaa ttatggtcat ctgaaagtcc cgtttgattc
    10501 actcatattg acccggaggt cgatcgatgc gaggaaaggt aaggttgtat atgtattgag
    10561 ataccaaaaa gtgtctgatg taccggccga taacggattc catgatctga ctccattggc
    10621 ggatcctgaa cattgcccac aagtggttgt ggtcggtgca ggacctgccg gttattttgc
    10681 agcctttcgc ctgatacaga gcggcataag gcctgttgtt gtcgaaaggg gcaaggatgt
    10741 gcaaagtcgc aggcgagacc ttagagccat catgcaagag agcattgtca atgaggatag
    10801 taattattgt tttggcgaag gtggtgccgg aacctattct gatggcaagt tatatacgcg
    10861 ttctacaaaa cgtggagatg tgaggcagat tctgaatacc ttttgttttt atggagctga
    10921 cccaagaata ctaatcgatg cgaaccccca tatcggcact aacaaattgc ccaagattat
    10981 taccgctatg cgggaagcta tattgaaagc aggtggaagg attctgttcg aacacagaat
    11041 ggttgatttt gatattgaag acggaagatt agccaaagta cactttcgac atcaaccttc
    11101 catcgaatgc aaggccatga tactcgccac agggcatagt gctcgtgata tttacagatt
    11161 gcttgccaat agaggattga agatcgaagc aaaaccgttt gcgatgggag tacgtgtgga
    11221 gcatccccaa tcattgattg acagtatcca gtatagttgc ccgaccagac ctgaaggtct
    11281 gcctgccgct ccatatagtg tggtgagcca ggaaggtggg cggggcgttt tttcattctg
    11341 tatgtgtccg ggtgggatga ttgtcccagc agcaaccagt ccgggagagc tagtcgtaaa
    11401 tggcatgtct ccatcccgaa gggacagtcc ctacgccaat agtggcttcg ttacgtctgt
    11461 cgagttggaa gatatccctg cagaatatgg agaagatgcc ttcgtagcca tgaatttcca
    11521 atcatccgta gaacagcggc tattcaatta cggcaatggt acacagtcaa gcccggcaca
    11581 aagactggtc gacttcacac gtaagaagga aagcacggac ctgcccaatt gcagttatgt
    11641 gcccggaatc tatccggcac cgatgcacag aatattaccg gaatttattg ttgacaggct
    11701 gatttcagga tttttatcag ttggcaaaaa actacatggt tacttgacca atgaagcagt
    11761 tgtcgtaggt gttgaaagca gaaccagttc tccaatccgc ataccaaggg ataaggaaag
    11821 tctccgacat cctgacctgg tcaacttatt tccctgtgga gaaggggcgg ggtatgcggg
    11881 aggcatcgta tcggcaggaa ttgacggtgt aaatgtggcc gtagcagtaa gtaaatatct
    11941 tactatggag ggatgaaatc cggaattgtt gggttggaag tttggtttgc cattcaggcc
    12001 gtttgtttta gaaatttggt aaggcattat tttactttgt ctacaacttt tgtacccatc
    12061 aattcaattg tattcatcaa catatcatgg ggaggagctc caacatcgat gtgacctatg
    12121 aatcgggtca agccgaaaac ctccttcatc atcaggattt tttcagaaac ttcctgtgag
    12181 ctaccaaata aataagctcc atgtttggac ctgccggctt cgtactgacc atgggtgaag
    12241 gttggccagc cacgactttg accgatacga ttcatttggc gactataata atgaaagtaa
    12301 ttatcgatgt ccttttggtt ctcacttaca aatgtatggg catgtac
//
LOCUS       DAOQQN010000038        12339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1791_length_12339_cov_5.399463, whole genome shotgun sequence.
ACCESSION   DAOQQN010000038 DAOQQN010000000
VERSION     DAOQQN010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12339
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1791_length_12339_cov_5.399463"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PLG24_02445"
     CDS             complement(<1..369)
                     /locus_tag="PLG24_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ23773.1"
                     /translation="MRRILFSIKDLKCSYDSGKIVLDIDRLDIYDGQITFIVGKSGSG
                     KSTLLETLGFMNDTFSSRTPEQVVFHDAGSNDKTDLASLWLKPLDMHSAFRNKNYSFI
                     FQENNLLEHFNSSENILIPIL"
     gene            complement(372..2921)
                     /locus_tag="PLG24_02450"
     CDS             complement(372..2921)
                     /locus_tag="PLG24_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23774.1"
                     /translation="MIKRYFSIFVFCFLVKGLSFGQAITLMEAPADYSPPLDYYFSGA
                     NNDLNLYINNQNLKPAGDKRSRRTWIIINDREGNIAYNGPSVTSGTKSTLGFLESFAV
                     SEVKGSFAHIFRIDGGVPHGKEIPAGAIAVDMGWVERSTVLQWGMGLVDSKSKISLKA
                     ILLNKAEDIKNLVGLSNKEIVRIYDSPYSKKTLGDRNIYEIFYILKKENGRALLSTEY
                     QFGSSPKTNIIGWVDLSRLSKWNTRIALEPNHNIMACEERKADENKRVSFFANSRDAN
                     QFLKTGDKDNILFSQDPVAISPEFLAKSNPRRYKGSILRYPILANPGNNLFQTGIIGK
                     VTTVGSGQESGSIDPAIYSKIVEAQRKRSEDNKIVNIFFLIEGSTEMNTYKSQLADLI
                     DKLPTNFPGANIVNVGCGIYRDFQSNQVPFTFRKLTQNRKDVAAFIDKEVFYRPLDDD
                     RETVFRFALKKTLEIHGGFQPGQRNILMIIGNAADFSNNTVRKLKTEKKEYLISGNEM
                     GSIYKALADLDMNIIFIQPHSEEGRFSRKFQSDGLELMGDVAGRIFSENEKISQKLKI
                     NLNGPEMPECQEGKLVEATGGVNYFGFYSAPISKELSPAKLMDEIKISLDACNKLRNS
                     HIKANADVIEKGKSLDVSPGEFKAYFMRFIKQELEDIDPASITKLLQEKYQLFIEAYL
                     PKHFNGAKYDSYSYVMFLPKNELYGFIQSLNDVAGKYNQGYSMSELRQSLKDAVTELV
                     LTVSSQNDRSRVGQLDMSSTLEIILGLKEEGVVLFQQYSNKKLADLTKKGVFPDEEVS
                     KLAKGFSDLKLKLEDIYKSGSRYEFCFRTQGDNDNIYFWIALEDIFDKSFF"
     gene            complement(2927..4468)
                     /locus_tag="PLG24_02455"
     CDS             complement(2927..4468)
                     /locus_tag="PLG24_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23775.1"
                     /translation="MKIRRIHIFWMIFGIILFSSNIFGQDCKLEKGDWSVIDRSGIEQ
                     MYKGKIRDALNQFLANYNRYADIYDEKYDWRESNFLKLLDKGNSTTPYLNDMLLHPTE
                     GMAIGYINQIAEYFPNGFKFCITGLTVKDIRALQSDLYFVKIQVVKRTENYVDAGQVV
                     NKERDITLDFDMIVFSMEDSEELTAAISSIELVRVSAAGAGGHDVAMEAPKVKKEPKQ
                     KQEKKEREPMAQPQDNYNSYEGKKKTRDFKMVYLDGGYGMPRNEVSAGIQESDFKSTG
                     FHIGLGGEYWKSFSPSNKVFYSIGLLFRYHNLKDEVVGSDVKLNTFSSHYNNNLSRAP
                     KTSEFRMQAGQISTNVVGVYAPIGLVYNFGRGNDPRVFMVQANIMPGYIFSSTRTVST
                     NSASIYSYYGDDSSIDNCEITAVKIDKNLTLKNRFTLAARLSPTYVYESKSWQGKRGF
                     GFVFGLDFTLGLMPVIVTENPQLIESYDSSNRTFTPISDASFFYQTKIYHSVGGRIGI
                     YYVLN"
     gene            complement(4491..8366)
                     /locus_tag="PLG24_02460"
     CDS             complement(4491..8366)
                     /locus_tag="PLG24_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ23776.1"
                     /translation="MNIRYNHSSLIIVVAWILSVATTFSCLGQAPSITIIASPGNSLC
                     VGDNLTMASTISDGGAFTFSYQWYKNNVEIPGATNDSYSIPNIQLIDAGTYKVVATYD
                     DMGNQEIDDQIIISVNPKPAITAIQKEDPTTCNGQDGSFTLVGLAGTGYQLFYTLNGT
                     EVGPINLPVNGEYKVSNLAAGNYTNIYVIKNNCKSNSQSVSLTDPPAPVITSVTGTNP
                     TTCGGQDGYFTVAGLSGTGYQLFYTLNGIEVGPINLPVNGEYKVSNLEAGNYTNIYVI
                     KNNCESNSRSVSLTDPPAPVIASVTGTNPTTCGGQDGYFTVAGLSGAGYQLFYTLNGI
                     EVGPINLPVNGEYKVSNLEAGNYTNIYVIKNNCESNSRSVSLTDPPAPVITSITGKNP
                     TTCGGQDGYFTVAGLSGTGYQLFYTLNGIEVGPVAIPVNNEYKVSNLAAGNYTNIYVI
                     KDGCKSNAREVVLTEPPKPIITNIKLTHPTTCDGADGSLELEGLTGTGFKAYYTIGGS
                     EKNIDLPSGGKFVISNLKAGSYSNIYVIKANCKSDPSSGPFELQDPGFPSTIVVDIKL
                     SDSIVCKGGTVIVTTSPAGGELKILSGDGFLSDSVLNTGSATGNTITLQYTYTDKNSK
                     CTKIVQKDVTVNPLPVLASEPDITHVRCNGEKTGSISLSPDDYTYRWSTGALGNSITG
                     LPAGNYSVTFTDANGCVGTRASIAITEPSKLTEDALQTEYVVCFGKSKEVTPQVNGGT
                     PPYSYTWVTPQGTSHNKSIVLDSSWTYKLTITDANSCELEKDINLVENDEIKLTLFSS
                     GGNKLQDHFCDGASITLKASGADSYEWGDNQMGSSIIVKEGGNYNVTGSKTQNNLKCY
                     VEKSIKITKDPLPEIVFSDGTNTGDTLKTCKGTSLNITVSNNKPSYSYSWSDGSTSST
                     RVLYEEKKYPITVTDNNTGCSDASTLDLQYKNRIFLQIVNVADIPIDTIVNFCTQPTK
                     TEVTFKLNGELGPKAYASATWWLDTDIIGNSKEIVIDFNKQTKNFKLKVKTFDQAGCE
                     MSQTISFIQNEPTSDIFDEYKCGVYKLKPGYSLEGSSLPSNIQDLGSGFYFITGKVNL
                     EYTLKNDAGECRSKMDKDFVSKITSGEFADDQFVIDECGPFLLARHKEGFCYDWYRLD
                     DQNKAIRIDSAGNSWLKVTQENLIKNKYIAIGYDCKNSCDSTFISTRSDGDEVVIPCG
                     GTDSKSLKAYPNPGSGHMYIQAAGLSPGKQTLDIYNMMGTRIYSMTIGQAESNLLTEV
                     SLLDIPNGIYLVRLGNDKAEVTGKIIVIH"
     gene            complement(8379..9605)
                     /locus_tag="PLG24_02465"
     CDS             complement(8379..9605)
                     /locus_tag="PLG24_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23777.1"
                     /translation="MKIKLSFYFSLCCILIACSSEKSNNAIKDTMTHFSINGKDVTAY
                     RMTNKDQFDASNKSNYASVGANEDISLTVDLPKDVDMDVAWYINGSEMKGSSVKTKFP
                     IPETYEVVLQIPGFEEVVKYIIVEEQSTPVDLAIQDTVTQEMPTTENLPETAPKEVEV
                     KKENKPLPIQNREVQSQKKQPSVVKNEKIETKKVQKRTTPSSEVNTQPEIVDGPPPPP
                     KRVVTESSPSKSPPVKSEPTTGFNNKKTEGAPKPGSTTDVSKKKTEESSKSGTTIDLN
                     NKKTEGAPKTEPCKTATYSSTASVSLKPKQDIELSSFYVYAAQSGNVDIILMENNKQV
                     DKLVGRHVNAGMTQLRVSTLNTTLKGGKVYVLKITGHGGITFANLAPCGVGGGGSILN
                     PSYPGGVSIFNIHFNY"
     gene            complement(10702..>12339)
                     /locus_tag="PLG24_02470"
     CDS             complement(10702..>12339)
                     /locus_tag="PLG24_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ23778.1"
                     /translation="KVIVGSIVRSSDKILPEGWKYTGNNHSNNSICKVPDCTDPSVFS
                     NLVLGNNDINGINFGIIGAYSLSGRVFHDADGLNKNGVNGNPVYPSSSYVQQGLPPLF
                     VCAVDKKGKVANYTSVRNDGTYQINIPIKQDITLLLTTTLPTIGGPSPLPSLPSGWTF
                     VGETFGTNNTLGSGNNDGSGSGANSPMTRYDGKVSINIQGNESSVGEVDFGIQQLPGA
                     DPKSYSVNISDFSSIPPNGYPIITNFKAITMSSPKLKESGNNSNGSLSGSDPEDCAAD
                     GTCNGNQGGIRTTFTIGTIHSNTRLYYDFGGVVGIREILANSVISNFNADKMVIYGQD
                     GEGNTHNPFGFEYTITDKAGTNGVFVTYGIQLNAPLPLNLLSFDVVRKEAAAYLSWTS
                     LDERQFRGFEVLRSTDGRIWTTVTFVPSNGISGARQDYFFIDHKPAEGLNLYKLKMID
                     QDEKYQYSDIRSVTFNIKNHASVYPNPTDGHLNITGLSGNETIRVFDLTGKVLMKVDA
                     KDQQGGMSLTGYSAGTYFVHITYVDGKTEIIKVEKIK"
BASE COUNT         3393 a         2694 c         2342 g         3910 t
ORIGIN      
        1 taatatggga attaggatat tctcactgga gttgaaatgt tccagcaagt tgttttcctg
       61 gaaaataaag ctataatttt tatttctgaa tgctgagtgc atatctaaag gcttgagcca
      121 caatgatgca agatccgtct tatcatttga tcctgcgtca tggaagacta cctgttccgg
      181 agtgcgggag ctgaaagtat cattcataaa gcccagggtc tcaagtagcg tactctttcc
      241 tgacccggat ttgccgacaa taaaggtaat ctgcccatca tatatatcca ggcgatcaat
      301 atcaagtact atcttgcctg aatcgtagct acatttcagg tcctttatgg aaaacaatat
      361 tcttctcatg cctaaaaaaa acttttatcg aagatatcct ctaaagcgat ccaaaaataa
      421 atattgtcat tatcgccttg tgttctaaag cagaactcat acctactgcc actcttataa
      481 atatcctcca gtttaagttt cagatcgctg aaccctttgg cgagcttact tacctcttca
      541 tccgggaaca ctcccttttt ggtaaggtct gccaacttct tgttggaata ttgctgaaac
      601 agcacaacac cttcttcctt caagcccaga ataatttcaa gcgttgagct catatcaagt
      661 tgaccaaccc tcgatctgtc attttgtgag ctgacggtca gaaccaactc agtaacagca
      721 tcttttaagg attgcctgag ttcggacatg gaatacccct gattgtattt acctgcaaca
      781 tcattgaggc tttgaataaa tccatacaat tcattcttag gaagaaacat cacataagaa
      841 taggaatcat acttggctcc attaaaatgt ttgggcaaat aggcttcgat aaaaagttga
      901 tacttttcct gaagcaactt agtgatggaa gcaggatcaa tatcttccaa ttcctgcttg
      961 atgaatctca taaagtatgc cttaaactct ccgggactta catccagact cttccccttt
     1021 tctatcacat cggcattagc ctttatatga ctattcctta atttattaca agcatccaga
     1081 gagatcttga tttcatccat caacttagcc gggcttagtt ctttactgat tggagccgaa
     1141 tagaatccaa aataattaac accccccgta gcctctacaa gcttgccttc ctgacattca
     1201 ggcatttcag gaccattcag gttgatcttc aatttttggc ttatcttttc gttctcactg
     1261 aatatcctcc ctgcaacatc acccataagt tccaggccat cagactgaaa ctttcgcgag
     1321 aatctgcctt cttcagaatg tggttggata aaaataatat tcatgtccag atcagccagt
     1381 gccttgtata tgctgcccat ttcattgcct gaaatcaaat attccttctt ctctgttttt
     1441 agcttgcgca ctgtattatt tgaaaaatca gcagcatttc ctataatcat caggatgttt
     1501 ctttgaccgg gttgaaagcc accatgtatc tccaatgttt ttttcaaggc aaatctgaac
     1561 acagtttccc tatcatcatc caatggacga tagaaaacct ccttatcgat gaaagctgca
     1621 acatcctttc tgttttgagt taattttcta aaggtaaaag ggacctgatt cgactgaaaa
     1681 tcgcgataga taccacatcc tacatttact atattagctc ccggaaagtt ggtaggtaat
     1741 ttgtcaataa ggtcagctaa ctgggatttg taggtattca tttctgtact tccttctatc
     1801 aggaagaaga tatttactat tttattatcc tcgcttcttt ttctctgagc ttctacaatc
     1861 ttactatata tggcggggtc aatcgatccg gactcctgtc cactcccaac agttgttacc
     1921 ttgcctataa tgccggtttg gaaaaggttg ttccccggat tagccaggat tggatacctc
     1981 aggatgcttc ccttatatct cctggggtta gacttggcaa gaaattccgg ggaaatagcc
     2041 acagggtcct gactaaagag aatattatcc ttgtctcctg ttttaagaaa ctgattggca
     2101 tcccggctat tggcaaagaa agaaaccctc ttgttttcgt cagcttttct ctcctcacag
     2161 gccataatat tatgattggg ctccagagcg atacgcgtat tccacttgga aagtcggctt
     2221 agatccaccc agccaataat attggttttg ggcgagctcc cgaattgata ttcagttgaa
     2281 agcagggcac ggccattttc ctttttcaat atgtagaaaa tctcataaat attcctgtct
     2341 cccagggtct ttttactgta tggactgtca tatatcctta ctatttcctt attactcaat
     2401 cccaccagat ttttaatgtc ttccgcctta ttcaacaaga tcgccttcaa ggatatcttg
     2461 gactttgaat caaccagacc cataccccat tgcaatacgg tacttctttc tacccagccc
     2521 atatccactg ctatagctcc ggccggaatt tccttgccat gaggtactcc accgtcgatc
     2581 ctaaagatat gtgcaaatga tcctttgacc tcactgaccg caaaggactc gagaaagcct
     2641 aaggttgact tagtcccgga ggtcacagaa ggtccgttat atgcgatatt cccttcccga
     2701 tcgttgatta taatccaggt ccgtcggctt cttttatccc ctgcaggctt gagattttgg
     2761 ttgttgatat acagattcag atcattatta gcgcctgaga aataataatc cagaggtggc
     2821 gaataatcag ccggtgcttc cattaaggtg atagcctgac cgaatgaaag ccctttaact
     2881 aaaaagcaga aaacgaatat cgaaaagtat ctttttatca ttgatgtcaa tttagtacgt
     2941 aataaattcc tatccttccg cctactgagt ggtaaatctt tgtttgatag aagaaggaag
     3001 catcacttat cggggtaaat gtcctgttgc tactatcata tgactctatc aactgtggat
     3061 tttcagtaac gatcaccggc ataagcccca gtgtaaagtc aagtccgaag acaaatccga
     3121 atcctcgctt accttgccac gatttactct catatacata ggtagggctc aatctggcag
     3181 caagggtaaa tctatttttt aaggtcaggt tcttatctat cttgactgct gtgatctcac
     3241 aattgtctat actggaatca tcaccataat aggaataaat tgaagcagaa ttggtgctaa
     3301 ccgtcctggt cgatgagaag atgtaacctg gcattatgtt ggcttgtacc atgaatactc
     3361 ttgggtcatt acctctgcca aagttataca ccaagccaat cggcgcataa acaccaacca
     3421 cattggttga tatttgtcct gcctgcattc tgaattccga tgtcttcggc gctctggaca
     3481 gattattgtt gtaatgggaa ctgaaagtat tcagtttgac atctgaacca acgacttcat
     3541 cctttaggtt atgatatctg aataataaac ctattgaata aaacacctta ttggaagggc
     3601 tgaagctttt ccagtattca cctcccagcc cgatatgaaa tcccgttgat ttgaagtcgg
     3661 actcttgtat gcctgctgac acttcgttgc gaggcatccc gtaccctcca tccagataga
     3721 ccattttgaa gtcacgtgtt ttctttttcc cttcatatga attgtaattg tcctggggtt
     3781 gtgccatcgg ctctctttcc tttttttctt gcttttgctt tggctccttt ttcacttttg
     3841 gtgcctccat tgcaacatca tgccccccgg ctccggctgc agaaacccta accaactcga
     3901 tggaactaat ggcagccgta agttcttctg aatcttccat tgaaaatacg atcatatcaa
     3961 aatccaatgt aatatcgcgt tccttattta caacctgacc ggcatccaca taattttccg
     4021 tccttttgac aacctgaatc ttgacaaaat aaaggtcgct ttgaagtgct cttatgtctt
     4081 tgacggtcaa tccggtaata caaaatttaa atccattggg aaagtactcg gcaatttggt
     4141 tgatatatcc tattgccatt ccttcagtcg gatgcaaaag catatcgttg agataagggg
     4201 ttgtggaatt gcccttatcc aacaatttga ggaagttgga ttcacgccag tcatactttt
     4261 catcatagat atcagcgtac ctgttataat tggcaagaaa ttgattcaag gcatctctaa
     4321 tctttccttt atacatttgt tcgatgcccg acctgtcaat aacggaccaa tctccctttt
     4381 ccagcttaca atcctgacca aaaatattgg aggagaaaag gatgatgccg aaaatcatcc
     4441 aaaaaatatg tattctgcgg atcttcatat tgaatctctt ttaacttttt ttaatgtata
     4501 actataattt ttcctgttac ttcggctttg tcattcccca atcttacaag atatatacca
     4561 ttcggaatgt ccaacagact gacctccgtc agcaagttgc tctctgcttg cccaatcgtc
     4621 atactatata tacgcgtgcc catcatattg tatatatcca gcgtttgttt gccgggagac
     4681 aaacctgccg cctggatata catatgcccg gagccgggat tgggatatgc tttcagactt
     4741 tttgagtctg tacctccaca agggattacc acttcatcac catctgaacg agtagagata
     4801 aaggtagaat cacaagaatt tttacaatcg taaccgattg cgatgtattt attttttatc
     4861 aggttttcct gggttacctt cagccaggag ttaccggccg aatcaattct gatagccttg
     4921 ttctggtcat ccagtctata ccaatcataa caaaaccctt ccttatgcct tgcaagtaaa
     4981 aacggtccac attcatcaat gacaaattga tcatcggcaa attccccact cgtaatcttg
     5041 ctcacaaaat ccttgtccat tttagatcta cattctccgg cgtcattctt aagcgtatat
     5101 tccagattta ctttgccggt aataaaataa aatccactgc ccagatcctg gatgttagaa
     5161 ggcaatgatg aaccttcaag agaatatccc ggttttagct tatagactcc gcatttatat
     5221 tcatcaaata tatctgaagt cggttcattc tggataaaag aaatggtttg agacatttca
     5281 cacccagcct gatcaaaagt ttttaccttt aatttgaagt tctttgtttg tttgttgaaa
     5341 tctattacga tctctttact gtttcctatg atatcggtat caagccacca cgtcgcactg
     5401 gcgtaagctt tgggaccaag ttctccattt agtttgaaag tcacttctgt cttggtaggt
     5461 tgggtacaga aattgactat ggtatcaata gggatgtcag ccacatttac aatctgcagg
     5521 aaaattctgt tcttgtattg aaggtccagc gttgaagcat cagagcagcc tgtattatta
     5581 tccgttaccg taatcggata ttttttttcc tcgtacaaaa ctcttgttga ggatgtgctg
     5641 ccatcgctcc atgaatagct gtatgagggt ttattatttg atacagtgat attcaatgag
     5701 gtgcccttac aggttttcag cgtatctcca gtattcgtcc catcactgaa gacaatctca
     5761 ggcagagggt ctttagttat ctttatggat ttctcgacat aacatttaag attgttctgt
     5821 gttttacttc cggtaacatt atagtttcca ccctctttga caatgatact agagcccatc
     5881 tggttatccc cccactcgta cgaatctgcc ccacttgctt tcagagtaat agatgcaccg
     5941 tcgcaaaaat gatcctgaag tttattacct cccgaggaga ataatgtcag cttgatttcg
     6001 tcattctcaa ccagattgat atccttctcc aattcacagg agtttgcatc ggtaatagtt
     6061 aatttataag tccaggagga atcgagaaca atagatttat tgtgggatgt gccttgaggt
     6121 gttacccatg tatagctata tggaggtgtc cctccattga cttgaggcgt cacttctttg
     6181 ctttttccaa aacagactac atattccgtt tgcaatgcat cttccgtgag cttgctcggc
     6241 tctgtgatgg ctattgaggc cctggtacct acacagccat tagcatctgt aaaagtaacg
     6301 gaatagttcc cggcaggcag tccggtgatg ctattaccta gggctccagt tgaccaccgg
     6361 tatgtataat cgtcaggaga aagagatatg ctaccagttt tctccccatt gcaccttaca
     6421 tgggtaatgt caggctctga agctaacacg ggcagtggat tgacggttac atctttttgt
     6481 acgatttttg tacactttga attcttatcc gtataggtat attgcaatgt aatcgtgttc
     6541 ccggtagcac ttcctgtatt caagacacta tcggataaaa atccgtcccc ggagagaatt
     6601 ttaagttcgc ctccggccgg gcttgttgtt acaataactg taccgccctt acatactatt
     6661 gagtcggaca atttgatatc cactacaata gttgaaggaa atcccggatc ttgcagttca
     6721 aagggtccag atgatggatc gcttttacaa ttcgctttta tgacataaat atttgagtag
     6781 ctacctgctt tgagattgga tattacaaac ttaccaccgc ttggtaaatc gatgttcttt
     6841 tcgcttccac caattgtata ataagctttg aatccggttc ctgtcaagcc ttccagctcc
     6901 agggaaccat cagcaccatc gcacgtggta gggtgggtaa gtttaatgtt ggttattatt
     6961 ggcttcggtg gctcagtcaa tactacttcc ctggcattac ttttacatcc atccttgata
     7021 acataaatat tcgtataatt tcctgccgca agattgctca ccttatattc gttgttaaca
     7081 ggaatggcaa caggccccac ttcgatgcca tttaaagtat aaaaaagctg atatcctgtg
     7141 ccacttaagc cggccactgt aaaatatccg tcttgcccac cacaagtagt agggttcttt
     7201 cctgttatgg atgtaatcac cggggcaggt ggatccgtta gggaaactga ccgggaatta
     7261 ctttcacaat tattcttgat gacataaata tttgtataat ttcctgcttc aagattgctt
     7321 actttatatt ctccattgac aggcaggttg attggcccaa cttctatacc attcagggtg
     7381 taaaaaagct gatatcccgc accacttaaa ccggccactg taaaatatcc gtcttgccca
     7441 ccacaagtag tagggttcgt tcctgttacg gatgcaatca caggagcagg aggatccgtt
     7501 agggaaactg accgggaatt actttcacaa ttattcttga tgacataaat atttgtataa
     7561 tttcctgctt caagattgct tactttatat tctccattga caggcaggtt gattggccca
     7621 acttctatac cattcagggt gtaaaaaagc tgataccctg taccacttaa gccggccact
     7681 gtaaaatatc cgtcttgccc accacaagta gtaggattcg ttcctgttac ggatgtaatc
     7741 actggggcag gtggatccgt tagggaaact gactgggaat tacttttgca attattcttg
     7801 atgacataaa tatttgtata atttcctgct gcaagattgc ttactttata ttccccattg
     7861 acaggcaggt tgattggccc aacctctgta ccattcaggg tataaaatag ctgatatcct
     7921 gtgcctgcta aaccaacaag cgtaaaggac ccgtcctgtc cattacaggt tgtagggtct
     7981 tctttttgta tggcagtaat cgctggtttg ggattgacgg aaattattat ctgatcatct
     8041 atttcctgat tgcccatatc atcataagtt gctactacct tataggtacc ggcatctatc
     8101 agttgaatat ttggaatcga ataactgtca tttgttgcac cgggtatttc aacattgttt
     8161 ttgtaccatt gataactaaa agtaaaggca ccgccatcgc ttattgtact tgccatggtc
     8221 agattgtctc ctacacaaag tgaattgcca ggcgaagcta taatggtgat ggatggagct
     8281 tgccccaaac aagaaaatgt agtagctact gaaagtatcc acgcaactac tatgattagc
     8341 gaagaatgat tatatctgat attcataata aaaaacagtt aataattgaa gtgaatatta
     8401 aagatggaaa caccacccgg ataggagggg ttcaatattg aacctccacc acctactcca
     8461 caaggggcga gattagcaaa ggtaatgcct ccatgtcccg taattttcag tacatacact
     8521 ttccctccct ttaatgttgt gttcagggtt gaaactctaa gttgtgtcat tccggcattg
     8581 acatgtcgcc caaccagttt gtcaacctgc ttgttgtttt ccatcaagat aatatctaca
     8641 ttacccgatt gggcggcata aacataaaaa cttgatagtt caatgtcctg tttgggtttc
     8701 agggacacag aagctgtcga agaataggtt gctgttttac agggttctgt tttgggagcc
     8761 ccctctgtct ttttgttatt taagtcaatt gttgtccctg atttggaaga ctcttccgtc
     8821 tttttcttac tgacgtcagt cgttgaccct ggcttgggtg ccccctctgt ctttttatta
     8881 ttgaagccag ttgttggctc tgacttgacc ggaggagact tgcttggact ggattcggta
     8941 actacccgct taggaggagg aggtgggcca tctacaattt ccggttgagt attcacttcg
     9001 gatgaaggtg tagttctttt ttgtaccttt tttgtttcaa ttttttcatt cttaaccacc
     9061 gacggctgct ttttttgtga ctgcacctct ctattttgga taggtagcgg tttgttctcc
     9121 tttttcacct cgacttcttt aggggcggtt tccggcaaat tttcagtggt tggcatttcc
     9181 tgagtcacag tgtcctggat cgccaaatcg acaggtgtac tttgttcttc aacaataata
     9241 tacttcacaa cctcttcaaa tccaggtatt tgaaggacaa cttcataggt ctccggtatc
     9301 ggaaatttgg tcttgacaga acttcctttc atttctgaac cattgatata ccatgccaca
     9361 tccatgtcga cgtctttggg tagatccaca gtcaaagaaa tatcttcatt ggcacctaca
     9421 gaagcataat tggatttatt ggatgcatca aactgatcct tattggtcat tctataagcg
     9481 gtaacatcct ttccattgat ggagaaatgt gtcattgtat ctttaatagc attattggat
     9541 ttttcgcttg agcatgcaat caaaatgcaa cataaagaga aatagaacga taattttatt
     9601 ttcatataaa aatatttata tcaaagattt cggtccttca agtattacgg acatcttccg
     9661 gggtttttac taaaatgcta tggattgctt ataactaaat atctgaagtg tcactcaatg
     9721 tgatattaag gcacaagtat tatagcgctc aaatataaca aaaatcgaaa aaatttatac
     9781 aattattatc ctttaattgt attggtaaaa tgaaactaat tatattcgga ggtttctgaa
     9841 taaaaatcaa tctgtggaat cgggttgagg agtatttgca ttacactgtc acatagctac
     9901 gctacaggaa tattgtagca tacttgtaca acctatatga gcttatcagg gcgttatggt
     9961 ataactaatt cgcttagata taacggtaag cctctatttg tccctgcatt tatgtaacag
    10021 agaaaaaaga atgccagctc atgatggaaa taggatgatg tattatgcat aagttgtgcg
    10081 attaatactc ctaacttttg tataaatgtt tttttattaa tatgtattga actgcttttc
    10141 ttttatttac aaaagaataa atgataggac ttatccagat atcaggaatt atccagatgt
    10201 ctattggtga aggtatttat acgaattttg ataaatattt ccggtatcct gcagctaatt
    10261 tcaaatttac tcattttgct ttaaccggcc aatcggcagg caggttcaac atgcactttt
    10321 tgagtatcag gtaatcagat ccaatcattg aatttacggc acttcaggca gtgttaatat
    10381 aagccggtgt ataatcctct agatacattc cgaagttcta cgatctctcc tacctctgat
    10441 gtcatgtata tagtattggg aagaatggaa ctgccgctaa ccaagagcga cttacaaaca
    10501 ctgaaaacat aaatattctg atttgattaa accgggagaa atagtaatac ctaaggtaca
    10561 ttcgttacta actcaatcag gctttttttg aggtaaccgg ctatcaaatt gtagttggct
    10621 gtaaggttat agtatgtatt caatcgagaa aggcagccat ttagactgcc tttcgatctg
    10681 tttcacaaga aatccaatta tttactttat cttctctacc ttgatgattt ccgtcttgcc
    10741 atcaacatag gtaatatgta caaagtaggt tcctgcactg tacccggtca ggctcatacc
    10801 gccctgttga tctttggcat ccaccttcat gagtaccttt ccggtcaaat caaacactct
    10861 gatggtttcg ttgccactca gccctgttat attaagatga ccatcagtag gattggggta
    10921 cacggatgca tgatttttaa tattgaaagt aacggatcgt atgtcgctgt actgatattt
    10981 ttcatcctga tcgatcattt tcagtttgta cagattgaga ccttctgcag gcttatgatc
    11041 aatgaaaaaa taatcttgcc gggcgcccga aatgccattg gaaggaacga aggtcactgt
    11101 agtccaaatc cggccatcag tacttctcaa gacttcaaac cccctgaact gacgctcgtc
    11161 aaggctagtc caggacaaat aggccgcagc ttccttccgc actacgtcaa aggacaacag
    11221 attcaaagga agcggggcat tcagttgaat accataagta acaaatactc cgttagttcc
    11281 ggctttatct gtgatggtat attcaaagcc gaagggattg tgcgtattac cctcaccgtc
    11341 ctgaccatat attaccatct tgtcagcatt aaaattagaa atcaccgaat tggccaatat
    11401 ttcgcggatg ccaacaactc caccaaaatc atagtacaat ctggtattgc tgtggatagt
    11461 ccctattgta aatgttgttc tgattccacc ctgattacca ttacaagtgc cgtcggcggc
    11521 gcaatcttca gggtcgctac ccgataagga accattggag ttattgccgg attccttgag
    11581 cttcggagaa gacatcgtaa tcgccttgaa atttgttata atcggatacc cgtttggcgg
    11641 gatagaacta aaatccgaaa tattgacact ataacttttg gggtctgcac ccggcaactg
    11701 ttgaattcca aaatcaacct caccgacgga cgattcattg ccctggatat taatcgaaac
    11761 ctttccatca tagcgggtca tcgggctgtt agcaccagat cctgagccat cattatttcc
    11821 gcttcccaag gtattattag taccaaatgt ctctccgaca aacgtccatc ctgaaggcag
    11881 tgaaggcaac ggagacgggc cccctatcgt cggtaatgtc gtggttaaca acagagtgat
    11941 gtcttgtttg atgggaatat tgatctgata tgttccgtca tttcttactg aagtataatt
    12001 ggctactttg ccttttttgt caacggcaca aacaaacaaa ggcgggaggc cttgctgaac
    12061 ataggaggag gaaggatata caggattgcc attgactcca tttttattca gaccatcagc
    12121 atcatgaaaa accctgcccg ataacgaata ggctcctatg ataccaaaat tgataccatt
    12181 aatatcattg ttgcctaaaa ccaaatttga aaaaacggat gggtcggtac agtccggcac
    12241 cttgcaaatt gaattattgg aatggttatt accggtatat ttccagccct caggcaatat
    12301 tttatcagaa cttcggacaa ttgaacctac aattacctt
//
LOCUS       DAOQQN010000039        12270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1810_length_12270_cov_7.371101, whole genome shotgun sequence.
ACCESSION   DAOQQN010000039 DAOQQN010000000
VERSION     DAOQQN010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12270
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1810_length_12270_cov_7.371101"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="PLG24_02475"
     CDS             complement(<1..968)
                     /locus_tag="PLG24_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HMZ23779.1"
                     /translation="MASLVIVHHSYDLLGIPTNNFFVRFTRGQLNLGSFAVGSFFIIS
                     GFLVTQSLLNSRSLTQYFTKRFLRLFPALFVSLFLSAVVLGPFLTHQSLSDYFLGDQG
                     TSPFRFIFLNFTLNIFGYNYNIRDLFQNNPIPYAVNGSLWTLKHEFVSYCILAGLSIF
                     GFIKHPKLLLVFTSLVGGGYLVHVFYNIDFFSGLTTEWWVFHSVEYPYLLLLLWLFLF
                     GSILYIYRDNIFVSTKMLVFISGVLYVALITGYLLPVWYVVFPYLLIGLALLLPMSWF
                     SKYGDFSYGIYIYAFPVQQALVYFLYPGIDVRRFMVYSFLITFVLSV"
     gene            complement(1148..2656)
                     /locus_tag="PLG24_02480"
     CDS             complement(1148..2656)
                     /locus_tag="PLG24_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23780.1"
                     /translation="MSIYIGLKEKISSLKYDRILDFFSGLLLLLLFLSAITRLDSAWD
                     SLAYHLPFAARQAGIISPDELRISQFLEQLYQGVPILAETLQGYLWRITGWITATNLI
                     SWGSLVIFIVVAALRLDLSFWKLTLLSLSVPLILLQSITSYNDLINGCFIALGIVGLF
                     AAVKNDSYNLRNYFFTLFPIMLSTNVKYQALPVGLFYTIIVIFVFLWKNWNRLILNKR
                     PSNFLISFILITVFLGAVGGFTLFKNFYFHHNPVYPLPLKIGPIEFPSNYTDLEFDVS
                     MSEKLKDLPRFQIYLISLSEIYLWTDNNGQLWGIGGMPGSLSENIFFKLGGFFVANLI
                     LWSIFLLTVFSYNPSKKKNFYFIVLITSFLFVGFLPSSGLLRYWLFLPLVFILVVLLF
                     YEELDNSQSLLKTMFVFIQLMLFAFVTYQNIDVYKFRERFNQTVTHDVSYAYLQTTYH
                     LEDMDSSVCIVHSRDNRLAFLYKITNMSFSIQDARSASDCIYENILILDQSQ"
     gene            complement(2755..3753)
                     /locus_tag="PLG24_02485"
     CDS             complement(2755..3753)
                     /locus_tag="PLG24_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HMZ23781.1"
                     /translation="MKKNLSDLIRFMIGLVISLVVGAVVFYKVDWYKTLMAFMNFKWI
                     AIPQFILAYFLSTLARAMASRMLLEKQPTINQSFVSLVEGYLLNNLFPFRLGEVGRAY
                     FLGRKSNISVFRVLSANFVERILDLIFVTSLLVSTFPFVFEMEWIRPVTFTTLAGMIL
                     VSIFLYIIVRFRERFQKISEAGLKRFPIAEKFILPKLHLAIDGLSVLGDFSKSANVFV
                     WMAICWFFNIAGSFIVLKQFVPSAPPYWAIFIVGVVAFAGAIPSSPAALGVLEGAVVM
                     ALSILGIASSTALAFALVWHFLVIGIAFVLGLYAFSKETESVWKIYEQLLTQRKSD"
     gene            complement(3994..4854)
                     /locus_tag="PLG24_02490"
     CDS             complement(3994..4854)
                     /locus_tag="PLG24_02490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ23782.1"
                     /translation="MTNCRLCNSDSLRDRFTVKEKYTICGCTVCGLVQLTYQPTDAEI
                     NAIYQDIYFSHGKYKDNDTLAKENIRRLSLLKKYVPGKSKVLEVGCGMGEFIHYSKSI
                     FDMQGFDISEHAIKIAKERNPELADRFWSGQMKNQHFEAESFDAICMWDVIEHLWDFN
                     PDIEKLVNALKPNGYLFISTPNIGSWNSKLTGKYWAMMTPPEHMSFFNRSSMYYLFNK
                     IVGPNTKVLEWHSKGKWVNVGFMLYKVNRIKAGMIPESMMKIFEKGPLSKLSIYVPTQ
                     DIQYVVVKKS"
     gene            complement(4872..5777)
                     /locus_tag="PLG24_02495"
     CDS             complement(4872..5777)
                     /locus_tag="PLG24_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ23783.1"
                     /translation="MDLSIHKKIGEVVSIIIPAYNEESGIGAVLDSLSHEEALKHAEI
                     IVVDDASTDRTSEVVGKYSNVKLVRHRINKGYGSAVVSGIKESSREYVFWFDADGQHR
                     VVDLLEVARKMITDDLDYCIGVRTAGSYEEPSRVLGKAILRFAVKMVAKRKISDYNSG
                     LRGFKKKIISKYLPLFPKRFGASTFTSLLMAERDYMGDEVDIVVVQRVGKSSVKIFTD
                     GFRTLLIILRLVLLFAPLKFWGNVGMLLILSGGGYGLIKMLLSEERLGFPVFASLVIL
                     LGVQALFFGLLSDQISAARLESLEK"
     gene            complement(5800..6771)
                     /locus_tag="PLG24_02500"
     CDS             complement(5800..6771)
                     /locus_tag="PLG24_02500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ23784.1"
                     /translation="MNIFKQSKKEPVGGNEIIQREKELTAQIQEAQAQASQAQAELEE
                     LKKVSGLFPPGHFYSPIPDLEEVRKHESSIWSVLPREIEGIDLNDKGQLQLLKKFSKF
                     YEELPFKDEKLEGLRYFFQNPAYSYSDAIVLYSMIRFAKPRHIIEVGSGFSSCVTLDT
                     NEVFFDGKIKTTFIEPFPNLFYSLLKEGDREKVRVLPIPLQDVSLAEFDELGKNDILF
                     IDSTHVSKINSDVNYIFFKILPRLKSGVIVHFHDIFYPFEYPKDWIYEGRAWNEDYLL
                     RAFLQYNSAFQVMYFNAFMGSLYEDQLQRYMPLCLKNRGGSIWLRKL"
     gene            complement(6807..10700)
                     /locus_tag="PLG24_02505"
     CDS             complement(6807..10700)
                     /locus_tag="PLG24_02505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ23785.1"
                     /translation="MSVVLTSYNHAKYLAEAIDSVLNQTFTDFELIIWDDASTDTSWD
                     IISSYQDTRIRAYRNETNQIVEGVRKAISEVAIGDYIAIHHSDDVWESQKLEMQVAFL
                     DKNPQIAAVFSNAKIINEEGSLFSDEGHFYYNIFDQPNRNRYEWLNYFFTQGNALCHP
                     SILIRKECYEEIGLYRSGLVQTPDFDMWVRLCMKYEIYVLPEKLIQFRIRTNEANASG
                     SRLDNNVRSQFEYFQVLDNYRQLSSYEEISKIFPAAEKYYRADGFDAQFVLGMIALEV
                     GTYKFTELFGLQLLFEALNNPTRAKKIKDLYDFDILKFLQLTGSHDVFSFGLREQADR
                     ELSKALQVLNERIAEREEAVSILNNRVAEREESINILNDRVAEREKAIQILNERAAER
                     EENIASLSIQINEHKNIISILNRDLKALGEKTTSLEQVQSQLESIYKSRSWKIASRLQ
                     RVWVMLLAGWRLPVTLLRKLINLPSLGRLKDFLKKYRDMHWIRRSGLFDEVWYLDNNP
                     DIAKSGINPLFHYLKKGGYEGRDPSPHFDSDWYLLTYPDVKISGENPLINYLRAGRAE
                     GRSAQDPSLDIVKNSGLFDAKWYLSAYPDVANSHIDPLLHFVRNGGFEGRDPGPQFNS
                     NWYLKTYKDAMGSNPLLHYLQVGRAEGRLTQDPDFTLVKNSGFMDEQWYLSHNPDVAA
                     AKMDPLWHFIKYGGLEGRDPGPKFSISAYLDVVFEDIKQAKINPLVHYLKHYPWDASR
                     LETGDGRMKPTIRRPLVSIVIPVYNAVDMTRACVESIYRETKGIPFEVIIVDNASQDG
                     TAEYLDMEKQNRPYFYVVSMPQNIGFGPAVNEGMQRSKSEYVVILNNDTMVSPNWLKH
                     LLAPFEKDAFIGITSPVTNYVGEGPQIDMEALEMPPDDVRIREHAASIAERTEIFIEP
                     NRLVFFCVAIRRELIDIIGYLDPGYEKGNFEDDDYCLRTRMAGYRLAIAKNAFVYHHG
                     SFTFKSNKISHNWYMEKNRGRFYRKAGRIATTLKQNLPDRLTTKHEVSVILRTKDRPL
                     LLQKAMTSLASQTFRDFEVVLVNDGGVDVSGLVNLFKPQFPIQYVYHPTSKGRTAAVN
                     AGIYSSTGRWITYLDDDDILYPWHLETLFQATGDGKHKFVYSDYNQALFLDMDAVSPT
                     VLKGMPPREYDRRVLLVQNYIPIHTWLHARECAEKAGAWDETLDRLEDYEFLLRVSEN
                     YSFQHVRKVTCEYRYYLSGANSIYSDRRRSMQALEHIYKRYPVADSELQKERQNILVA
                     IEKQINKIEEIRSQIGTSITEDRAVREIIKLVANI"
     gene            complement(10794..11990)
                     /locus_tag="PLG24_02510"
     CDS             complement(10794..11990)
                     /locus_tag="PLG24_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ23786.1"
                     /translation="MKNNITELALLGGTPLFADTLHVGRPNIGNRDRLLARINDMLDR
                     RWLSNGGPFAQELEMKLADFLGVKHCIVMCNATIALEIAMRALGLTGEVILPSFTFVA
                     SAHALQWQEITPVFCDVNPDTHNIDPSKIENLITPRTTGILGVHVWGRACDIDALSAI
                     AKKHNLKLMFDAAHAFGCTYRGQMIGGFGNVEGFSFHATKFFNSFEGGVVTTNNDELA
                     KKIRSMHNFGFAGLDLVTEIGTNGKMSEAAAAMGLTSLESLDDFIKVNQDNYRIYSQE
                     LSDLPGIKLIRYNEDERNNYQYIVVEINEELTGISRDLLVRVLHSENILARRYFYPGC
                     HKMEPYRSYFPNAGLVLQETEKLNLSVLQLPTGTAVGQEEISKICQLIRFVTTNGHEI
                     RTSVVD"
     gene            complement(12003..>12270)
                     /locus_tag="PLG24_02515"
     CDS             complement(12003..>12270)
                     /locus_tag="PLG24_02515"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HMZ23787.1"
                     /translation="DVSLSAEERILDICEYFGATEYVNLPGGVDLYHPEAFERRNIKL
                     TFRNLPTFVYPTGPYAFEPNLSIIDLLMWNKPEDIRKHLEEFRS"
BASE COUNT         3940 a         2656 c         2240 g         3434 t
ORIGIN      
        1 acggaaagaa caaaggtgat caaaaaggaa tagaccataa aacggcgaac atcaatccca
       61 ggatagagaa aatataccaa ggcctgctga accggaaatg cataaatata gatgccgtag
      121 gagaagtcac catattttga aaaccaagac atgggcaaaa gcagggccaa cccgataagc
      181 aaataaggga aaacaacata ccaaacagga agaagatatc cggttatcaa tgctacatat
      241 aatactcctg aaataaacac caacattttt gtgctaacaa aaatgttatc ccggtatatg
      301 tatagaattg aaccaaacag gaatagccaa agcaataaca agagataggg atattcaaca
      361 ctatgaaaaa cccaccactc tgtggttaag ccactaaaaa aatcaatatt ataaaaaaca
      421 tgcacaagat atccaccgcc caccaggctg gtaaatacca aaagcaattt agggtgtttg
      481 ataaaaccaa atatggagag cccggctaaa atacaataac tgacaaattc atgttttaac
      541 gtccagaggg agccatttac cgcatatgga atggggttat tttgaaacag gtcacgaata
      601 ttgtaattgt agccaaaaat attcaacgta aaattgagaa aaataaagcg aaagggactt
      661 gtaccttgat cgcccaggaa ataatccgaa aggctttgat gggttaggaa agggcctaat
      721 acaactgctg acaagaataa actgacaaat aaagccggga ataagcgtaa aaaacgtttt
      781 gtaaaatatt gagtcaaact cctgctgtta agcaggcttt gagtgaccaa gaagccgctg
      841 ataatgaaaa agcttcccac tgcaaaagag ccaagattta attgtcctct cgtgaaccgc
      901 acaaaaaaat tatttgtagg aatacccagt aaatcatatg aatgatgaac aatgacaagc
      961 gaagccatga caaacctcag aatatcaaag gcattgctgt ttggctggta tatttcagat
     1021 attttttttt cgaaattcca aatttgtttg atcttttcaa atacaggcat gccaactcct
     1081 ttttgtaata tgatcatcgc tttttatata gttagagaga aaatctcaag atgcatacaa
     1141 aaataaatta ttgactctga tctaaaatca gaatattctc ataaatgcaa tcactggcac
     1201 ttcttgcatc ttgtatagaa aaagacatat tggtaatttt gtataaaaat gctaaacgat
     1261 tatcacgact atgaacaatg cacactgaag aatccatgtc ttccaaatga taggtggtct
     1321 gtaaataagc ataacttaca tcatgcgtta ctgtttgatt aaatctttcg cgaaacttat
     1381 atacatcaat gttttggtag gttacaaacg caaacaacat tagctgaata aatacaaaca
     1441 tagtcttaag caaagattga ctattgtcta gttcttcata aaagagtaat actactaaaa
     1501 tgaataccaa cggcaggaat aaccaatatc gtaaaagacc tgatgaaggc aaaaaaccga
     1561 caaataaaaa gctagtgatc aaaacaataa agtaaaagtt cttcttctta cttgggttat
     1621 aactgaaaac agttaataaa aaaattgacc ataaaatcaa gtttgctaca aaaaaaccac
     1681 ccagcttaaa gaaaatattc tcgcttaagc tgccaggcat gccgccaatt ccccataatt
     1741 gcccattatt atctgtccac aaataaattt ctgaaagaga aattaagtat atttgaaatc
     1801 ttggtaaatc tttcaacttt tcagacatgg atacatcaaa ttccaaatct gtataattag
     1861 aaggaaattc aatgggacca atttttaaag gaagagggta gactgggttg tgatgaaaat
     1921 aaaaattctt aaacaaagtg aatccgccaa cagcgcctaa aaatactgta attaaaataa
     1981 aggaaatcag gaaatttgaa ggtcttttgt ttaatatcag gcgattccaa tttttccaca
     2041 aaaagacaaa aatcacaata atggtgtaga aaaggccgac agggagggcc tggtacttca
     2101 catttgttga taacatgatt ggaaataatg taaaaaaata attccttaag ttataagaat
     2161 catttttaac tgcggcaaac aatccaacaa ttcctaatgc aataaaacag ccattgataa
     2221 gatcgttata acttgtaata ctttgcaaca gtatcaaagg gacgctcaaa gaaagtaatg
     2281 tgagcttcca aaaacttaaa tccaagcgca aggcagccac aacaataaat ataaccagac
     2341 taccccagga aattaaatta gtagctgtaa tccaaccagt aattcgccaa aggtatcctt
     2401 gtaaagtttc agccaatatg ggcacccctt gatacaattg ttcgagaaac tgagaaatcc
     2461 tcaactcatc tgggctaatg atccctgctt gccgtgctgc aaaaggtagg tgataagcca
     2521 gactatccca ggcagaatcc aatctagtta ttgcagatag aaacaataac agcaacagca
     2581 aacctgaaaa aaaatctagt atccgatcat attttaacga agatattttt tcctttaagc
     2641 caatatatat tgacatggcg ttcctctttt tatattttat taatagttag attcaaagta
     2701 gaaatttctt agaagttcga gagcttaaag ttccaagtaa tataaatcaa aggtctagtc
     2761 actttttcgt tgtgtcaata attgctcata tattttccaa acagactcag tttctttgga
     2821 aaacgcatac aggccaagaa caaatgctat tccaatgact aggaaatgcc aaaccaacgc
     2881 aaatgccaat gccgtactag atgctatacc caaaatggaa agagccataa cgactgcacc
     2941 ttctaaaacg ccgagggctg cgggagatga ggggattgcc ccggcaaagg ccactacacc
     3001 gacaataaaa atagcccaat atggcggggc agatggtaca aattgtttta atactataaa
     3061 agatccagca atattaaaaa accaacaaat ggccatccat acgaatacat tggcggattt
     3121 actgaaatcg cccaagacag atagtccatc aatggcaaga tgtaatttag gcagaataaa
     3181 tttttcggca atgggaaacc gtttcaggcc agcttctgat attttttgaa acctttccct
     3241 aaaacgtaca ataatgtata ggaaaatcga caccaatatc atgcccgcga gtgtggtaaa
     3301 tgtaacaggc ctaatccatt ccatttcaaa gacgaaggga aaagttgaga ccaaaagact
     3361 ggtaacaaaa ataagatcaa gtatccgttc tacgaagttt gctgaaagaa cgcgaaacac
     3421 acttatattc gattttcgac ctaaaaaata agcacggcca acctcaccta gtcgaaaggg
     3481 gaaaaggtta ttcaacaaat atccttcaac gaggctaaca aaggattggt taattgtagg
     3541 ctgtttttcc aacaacattc gtgaagccat tgcccgtgcg agggtggaca gaaagtatgc
     3601 taaaataaat tgagggatag caatccattt aaagttcata aaagccatca acgttttata
     3661 ccaatcgact ttataaaaaa caacagcacc aaccaccaag ctaattacaa ggccaatcat
     3721 aaaacggatt aaatccgaaa gattcttttt catagaaaaa tactctcaaa aggaatagtt
     3781 actttgtctg gtgtgatctt gctaagataa tatatcatac gcctctagtt ttgattactt
     3841 tatagtaaat gacactgaaa aggtaaaagc tatgatagta actaaaacaa gacttatacc
     3901 attagcaatt gcaaaatgaa tccatgagcc attctgtccc actccaacaa tcacaccatc
     3961 atatctgcta ataggggtta aacagtttta cacctaagat ttcttaacaa caacatattg
     4021 aatatcctga gtaggaacat agatagataa ttttgacaag gggcctttct cgaatatctt
     4081 catcatactt tctggaatca taccggcttt tattctgttt actttataaa gcataaaccc
     4141 cacattcacc cactttcctt ttgaatgcca ttccaaaacc tttgtattgg gtcctactat
     4201 cttattaaat aaataataca tactgcttct attaaaaaag ctcatatgct ccggaggcgt
     4261 catcattgcc caatatttgc cggtcagctt tgaattccac gaaccaatat taggagtaga
     4321 tataaataaa tacccattgg gtttcaaagc attgaccaat ttctcaatat ctgggttaaa
     4381 gtcccaaaga tgctcaatta catcccacat gcaaatggca tcaaaagatt cggcttcaaa
     4441 atgctgattc ttcatttgcc ctgaccaaaa tcgatctgcg agttcagggt tcctttcttt
     4501 ggcaatctta atggcatgtt cagaaatatc gaagccttgc atatcaaaga tggatttgga
     4561 ataatgaata aattcaccca ttccacatcc tacttcaaga acttttgatt ttccaggaac
     4621 atatttctta agcaaagaca atcttcgaat attttctttc gccaaagtat cattatcctt
     4681 gtatttaccg tgactaaaat aaatatcctg ataaattgca ttaatttcag catcggttgg
     4741 ctgatatgtc aattgaacta aaccacaaac ggtacaacca caaatcgtat atttttcttt
     4801 gacggtaaac ctgtcacgaa gagaatcgct attacaaagg cggcaatttg tcatttatcc
     4861 atttcctcta tttatttttc caaactttcc aaacgcgcag cgcttatctg atcgcttaat
     4921 aatccaaaaa agagggcttg aacgcctaat aagatcacca aagaggcaaa aaccggaaag
     4981 cccaatcgtt cttcagacag gagcattttt atcaatccat acccgccccc cgataagatc
     5041 aataacatgc caacattccc ccaaaattta agtggggcaa acaacaaaac gaggcgaaga
     5101 ataatcagca aagtccgaaa cccatcggta aatatcttta ccgagctctt gcccacccgc
     5161 tgaaccacaa caatatcaac ttcgtcgccc atataatccc gttcagccat caacaaggat
     5221 gtaaatgtag aagcgccaaa acgtttggga aatagaggta aatatttcga aatgatcttc
     5281 ttcttaaatc cccttaaccc cgagttgtaa tcagatattt ttcgtttcgc gaccattttt
     5341 acagcaaaac gcagtattgc ctttcctaaa acccggctgg gttcctcata ggaacctgcc
     5401 gttcgaacgc caatacaata atccagatca tccgttatca ttttccgggc aacttccaac
     5461 aggtccacaa cccggtgctg cccatctgcg tcaaaccaga aaacatattc ccggctggat
     5521 tctttgatcc cgctaacaac agccgaacca taccctttat tgatgcgatg tcgcaccaac
     5581 ttaacatttg aatacttgcc aaccacctca gaggttctat cggtagaagc atcatccaca
     5641 acgataattt cagcatgctt taaggcttct tcgtgagata aggaatcgag aaccgcccca
     5701 attcccgact cctcgttata agcagggatg atgatggata ccacctcgcc aatttttttg
     5761 tgtatgctta aatccattct acaataacca atgattcctt tacaattttc ttaaccaaat
     5821 actgccgccc cggttcttta aacacaaagg catgtatctc tgaagttggt cttcatagag
     5881 actgcccata aaggcattaa agtacatgac ttgaaaggcg ctattatatt gtaaaaatgc
     5941 cctcagaaga taatcctcat tccaggcccg gccttcatat atccaatctt tgggatactc
     6001 gaacggataa aaaatatcgt gaaaatgaac aatcacaccg ctctttaagc gcggaaggat
     6061 tttgaaaaag atgtaattta catcgctatt gatcttgcta acatgggtcg aatcaataaa
     6121 gagaatatcg tttttaccca attcatcgaa ctcagccagg gatacatcct gcaatgggat
     6181 cggcaacacc cgaacctttt cccggtcccc ctccttcaac aaggagtaaa acaggttggg
     6241 gaaaggctca ataaaagttg ttttgatttt accgtcaaag aatacttcat tggtatccag
     6301 ggtaacgcat gaagaaaagc cgctgcctac ttcgataata tgacggggtt tggcaaatcg
     6361 aatcatgcta tacaacacga tggcatctga ataagagtat gctggattct ggaaaaagta
     6421 tctcaagccc tccaactttt catccttaaa cggcaattcc tcatagaatt tactaaactt
     6481 cttaagcaat tgcaattgac ccttatcatt caggtcgatg ccctcaattt cacgaggaag
     6541 aacagaccaa atggaacttt catgtttacg gacttcttcc aaatcaggga tgggggaata
     6601 aaaatgtccg ggaggaaata aacctgatac ttttttaagt tcctcaagtt cagcttgggc
     6661 ctgggaagcc tgagcctgcg cctcttgaat ctgagccgtc aattcttttt cgcgctgaat
     6721 tatttcatta ccgcctacag gttctttttt tgattgtttg aatatgttca tgcgttctcc
     6781 aaaatttgac gtatcagtgt ttatatttat atatttgcaa ccagtttaat aatttctcga
     6841 acagcccgat cttccgtaat ggatgtgcca atttgtgatc gaatttcctc gatcttattt
     6901 atctgcttct cgatcgcaac aagaatattt tgacgttctt tttgcaattc cgaatcagcg
     6961 accgggtaac gcttataaat atgttcaaga gcctgcatag accgacgcct gtcagaataa
     7021 atggaattcg caccgcttaa ataatagcga tattcgcaag taactttccg gacatgttgg
     7081 aatgaatagt tttcactgac acgcaacagg aattcataat cttccaatcg gtcaagagtc
     7141 tcatcccatg ctccagcttt ttcggcacat tcccgcgcat gcaaccaggt gtggatcgga
     7201 atgtaatttt gaacaagcaa caccctgcgg tcatactctc tgggaggcat gcccttaagc
     7261 acagttggcg atacggcatc catgtctaga aacagcgcct gattataatc gctataaacg
     7321 aatttatgtt taccatcacc ggttgcctga aaaagagtct ccaaatgcca ggggtacaaa
     7381 atatcatcat catcaagata ggtaatccat cggccggtac tggaataaat gcctgcattt
     7441 acagctgcgg tgcgcccctt tgatgttggg tgatatacat attgaatggg aaattgcggt
     7501 ttgaacaggt taaccaggcc ggaaacatcg acgccgccat catttaccag cactacctca
     7561 aaatctcgga atgtttggct ggccaggctg gtcattgcct tttgaagcag aaggggccgg
     7621 tcttttgttc gcaaaataac actgacctca tgttttgttg ttaatcgatc aggtaaattt
     7681 tgctttaagg tggtcgctat tctgccagcc ttcctataga aacgtcctct gttcttttcc
     7741 atgtaccagt tatgcgaaat tttattggac ttaaaagtaa aacttccatg atgatacaca
     7801 aaggcgtttt tcgcaatcgc aaggcggtat cctgccattc ttgtgcgtaa acagtaatca
     7861 tcgtcttcaa aattaccttt ttcataacca ggatccagat aaccgattat gtcgatcaat
     7921 tcccgccgaa tcgcaacaca gaaaaagaca agccgattgg gctcaataaa aatttcagtt
     7981 ctctctgcaa tggaagcggc atgttcccga atacgcacgt catcaggcgg catttccagg
     8041 gcttccatgt cgatctgcgg accttcgccc acgtaatttg tgacagggct tgtgatccca
     8101 atgaatgcat ccttttcgaa tggagccagc aaatgtttca gccagtttgg ggataccata
     8161 gtgtcattgt tcaggatgac aacatactca cttttactac gctgcatccc ttcatttaca
     8221 gcagggccaa agccaatatt ttgaggcata ctcaccacat aaaagtatgg gcgattttgc
     8281 ttttccatat ccaaatattc agccgttccg tcttgtgatg cattatcaac aataataact
     8341 tcaaatggaa tacccttcgt ttcgcgataa atactttcaa cacaagcccg ggtcatgtca
     8401 actgcgttat atacagggat cacaatgcta accaaaggtc tgcgaatggt aggcttcatt
     8461 ctgccatcac cagtttccaa tcgagaagca tcccaaggat aatgtttaag gtaatgtact
     8521 aatgggttga tctttgcctg ttttatatct tcaaatacaa catccaaata tgcagaaatg
     8581 gagaatttcg gccctgggtc acgcccttcc aggccaccgt atttaataaa gtgccataag
     8641 ggatccattt tggcggcagc tacgtcaggg ttatgagaca ggtaccattg ctcatccata
     8701 aagccggaat ttttaacgag cgtaaagtcc gggtcctgag ttaagcgtcc ttccgcccga
     8761 cccacctgca ggtagtgaag aagcgggttg ctgcccatcg catctttata tgtcttcaaa
     8821 taccagttgc tgttaaattg gggcccgggg tcacgacctt caaagcctcc attcctcaca
     8881 aaatgcagca atgggtcgat atgtgagttg gcaacatctg gatacgcaga aagataccac
     8941 ttcgcatcga ataggccaga attcttaaca atatccagcg agggatcctg ggcgcttctt
     9001 ccctcagccc ggcctgcccg caggtaattt atcaatggat tctcaccgga aatttttaca
     9061 tctggatacg tcaataaata ccaatcacta tcaaaatgag gggatgggtc tcgcccttca
     9121 tatcctccct tttttaagta atgaaataat gggttaattc ccgattttgc aatatcagga
     9181 ttgttatcaa gataccatac ttcatcaaac aatcccgacc tcctgatcca atgcatatca
     9241 cggtattttt tcaggaaatc cttaagcctt ccgagagatg gcaggttaat gagttttctc
     9301 aggagtgtga cgggaagtct ccacccagcc aacaacatga cccaaacacg ctgcagcctg
     9361 ctggcgatct tccatgacct acttttatag atactttcta attgggattg cacttgttcc
     9421 agagaggtcg ttttttctcc caaagccttt aaatctctgt tcaatatcga aattatattt
     9481 ttatgttcgt tgatctgtat gctaagtgaa gcgatattct cttcacgctc ggcagcccgt
     9541 tcgttcaaga tctgaatggc cttttcgcgt tcagcaaccc gatcattgag gatgttaata
     9601 gattcttcgc gttcagcaac ccgattatta aggatactaa ccgcttcttc acgctctgca
     9661 atgcgttcat taagtacctg tagagcttta cttaattcgc ggtctgcctg ttctcgaagc
     9721 ccaaaagaaa acacgtcatg tgagcctgtt aattgcaaaa atttgaggat atcgaaatca
     9781 taaaggtctt ttattttttt tgcccttgta gggttgttca atgcttcaaa taaaagttgc
     9841 aagccaaata attcagtgaa tttatatgtt ccaacttcca gagcaatcat acccagcaca
     9901 aattgagcat caaacccatc ggcacggtaa tatttttcag ctgccgggaa gatttttgaa
     9961 atttcctcat agctcgataa ctggcggtaa ttatccaaga cctgaaagta ttcgaattgg
    10021 ctgcggacat tattatcgag tcggctgcca ctcgcattag cctcatttgt ccgtatccga
    10081 aactgaataa gcttttctgg gagtacataa atttcatact tcatgcataa ccgcacccac
    10141 atgtcaaaat caggcgtttg aacaagtcca ctgcggtata agccgatttc ttcataacat
    10201 tctttacgta tcaaaatact gggatgacaa agagcattcc cctgagtaaa aaaatagttt
    10261 aaccattcat accgatttcg gtttggctgg tcaaagatat tgtaatagaa gtgtccctca
    10321 tcactaaata aggaaccctc ctcattaata atttttgcgt tactaaaaac ggccgcaatt
    10381 tgtgggtttt tatccaaaaa tgcgacttgc atctccaatt tttgggattc ccatacatcg
    10441 tcagagtgat ggatggcaat ataatcccct atagcaacct ctgaaatggc ttttcgaaca
    10501 ccttcaacaa tctgatttgt ttcgtttcgg tatgcacgta tccgtgtgtc ttgataactt
    10561 gagataatat cccatgaggt atcggtagag gcgtcatccc aaatgatcag ctcaaaatcc
    10621 gtaaatgtct ggtttagaac actatcaatt gcttcagcca agtattttgc atgattatag
    10681 gaagttaaaa caacagacac cttggttttt tttgtcatta gagcaatgcc tcaaaatggg
    10741 tatggatgga ggattaacat aacctcccct cctaaaggag caggtattat atcttagtct
    10801 acaactgacg tccggatctc atgtccattg gtggttacaa aacggattag ctgacaaatt
    10861 ttggatattt cttcctgtcc tacagctgtt ccggtgggta gctgaagaac gcttaagttc
    10921 agtttttctg tctcctgtaa aactaatcca gcatttggaa aataagaacg ataaggctcc
    10981 attttatggc agcccgggta aaaataacgc ctggcaagaa tattttcaga atgaaggaca
    11041 cgcaccagca aatcgcggct aattccggtt aattcttcgt tgatctcaac aacaatatat
    11101 tgatagttgt tcctttcatc ttcgttatat cttatcaatt taatgccagg gagatccgac
    11161 aattcctgcg aataaatccg atagttatcc tggtttactt taatgaaatc gtcaagactc
    11221 tcaagggacg ttaatcccat ggcagctgcg gcctcgctca tttttccgtt tgttccaatt
    11281 tcagtaacga gatccagccc ggcaaagccg aaattgtgca tactacgaat cttttttgcc
    11341 agttcgtcgt tatttgtagt aactactcca ccttcaaaac tattaaaaaa tttggtggcg
    11401 tgaaaactaa atccctccac attcccaaac ccgccgatca tttggccacg ataagtacaa
    11461 ccgaatgcgt gtgccgcatc gaacattaat ttaaggttgt gtttttttgc aatggcgctt
    11521 aaggcatcta tatcacaggc ccgtccccat acatggacgc ctaaaatccc ggttgttcgc
    11581 ggtgtaatca ggttctcgat ttttgaaggg tcaatattat gtgtatccgg attcacatca
    11641 caaaatactg gcgtaatctc ctgccactgg agagcgtgcg cactggcaac aaacgtgaag
    11701 gaaggaagga tcacctcgcc agtcagcccc aatgcacgca tggcaatttc caaggcaatg
    11761 gttgcgttac acatcactat gcaatgctta acgcctagaa aatctgcaag tttcatctca
    11821 agttcttggg caaaagggcc gccgttgctt aaccagcgtc tatccagcat atcattaatg
    11881 cgagccagca gccgatctcg gtttccgata ttgggtctgc caacatgcaa ggtatctgca
    11941 aaaagcggtg tgccccccaa caaggcaagt tctgttatat tatttttcat catattcgaa
    12001 ttctaactcc gaaattcttc aaggtgtttt ctaatatctt caggtttatt ccacattaat
    12061 aaatcaatga tggataaatt aggttcaaag gcatatggcc ctgttgggta tacgaaagtc
    12121 ggaaggttac gaaaggtaag ttttatattt cggcgttcaa atgcttctgg atgatataaa
    12181 tccactcctc caggaaggtt gacgtattcg gttgccccaa aatattcaca aatatccagg
    12241 attcgttctt cggcactaag agagacatcc
//
LOCUS       DAOQQN010000040        12014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1865_length_12014_cov_6.607158, whole genome shotgun sequence.
ACCESSION   DAOQQN010000040 DAOQQN010000000
VERSION     DAOQQN010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12014
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1865_length_12014_cov_6.607158"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(454..1314)
                     /locus_tag="PLG24_02520"
     CDS             complement(454..1314)
                     /locus_tag="PLG24_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HMZ23788.1"
                     /translation="MKLIRFGQKGKEKPGILLDGKYRDVSSYIHDYDETFFENDGLQR
                     LEQIIHSSTTTMAEIPQDTRLGSPVARPSKIVCIGLNYKNHVMETNANIPTQPILFFK
                     STTALSGPNDDVMIPRDSVKTDWEIELAVIMGRRASYIEPEEAYDYIAGYSLINDYSE
                     RAFQKEMGGQFCKGKSCDTFAPMGPFLATKDEVEDVHNLSLTLSVNEIIRQQGSTADM
                     IFDIPYLVSYISRFMTLLPGDILCTGTPEGVGAGIKPEPVFLRSGDVVRMSIDELGSS
                     TQRVIAYSGG"
     gene            complement(1504..2076)
                     /locus_tag="PLG24_02525"
     CDS             complement(1504..2076)
                     /locus_tag="PLG24_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HMZ23789.1"
                     /translation="MNTIAINGNKRNEVGKKASKAVRSSEGIPCVLYGKNGTTHFTTQ
                     EAEVRHLVFTPEFKVAELNVDGAKSKAILKDIQFHPVKDTITHIDFLELTEGNPVKVE
                     IPLKTKGSSPGVKVGGKLIQSVRKIKVKTVPEKLVGELFVDISGLELGQSLRVKDIIA
                     QDGIEVMQNGSIPVVSIEIPRALRSAAGKE"
     gene            2364..3461
                     /gene="hisC"
                     /locus_tag="PLG24_02530"
     CDS             2364..3461
                     /gene="hisC"
                     /locus_tag="PLG24_02530"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HMZ23790.1"
                     /translation="MVIIPPHIENLAPYKPGKPGANMFEDAKPEREVVLSSNENNFGP
                     SPKAMEALKRSLNNMNLYPDPTASLLKERLSHLLDIPVSHLLMSNGSDAILYTMFRAF
                     LSGDDSMLTSHAAFVSAKVMARMNNIRCVEVAMTGDHEFDLDGIITNIDDTTKLIYLV
                     NPNNPTGQMMTRTAMDSFLSRVPQHILVVVDEAYCEFARALSGEYPDCTRLGYNNVIV
                     LRTFSKAYGLAGLRLGYAIGPEYLIQALTKVKLTFDPNLAAQAAGLAALDDTTFLDKT
                     IENNTTELQKLNQAYDRLGLKVIPSVANFITIKLKNEAEVENYYHQLLSRGVLTRRLA
                     SFGLPYCLRISIGKPDENDYMLRMLEEVMDQ"
     gene            complement(3526..4515)
                     /locus_tag="PLG24_02535"
     CDS             complement(3526..4515)
                     /locus_tag="PLG24_02535"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HMZ23791.1"
                     /translation="MSTKIKVAILAGGDTEERVVSVKSAGVVHKYLHGEKYDARIIDI
                     NGREWIDLDTGHSIDKNDFSLDLGGSKWKPEVVFCAIHGAPMENGIIQGYFDILGIPY
                     TCCDGFVSGLTMNKSLTKRTLQAYDIPMAREVLVTKAAYREGVDTEDIIAKLGLPLFV
                     KPNAHGSSFGVRKVKNIEELIPAIEYAFQFDQDVMVESMMAGREFGNGVMRINGEIVV
                     LPVTEIIPLKEFFDFEAKYEGASREVTPAEISSELERQVKEYTSKIYTALNCSGFVRV
                     DYILVGDTFHLIEVNTVPGLSEASIIPQQAIAHGWTLNQFFDLAVSEALREKS"
     gene            complement(5409..8315)
                     /locus_tag="PLG24_02540"
     CDS             complement(5409..8315)
                     /locus_tag="PLG24_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ23792.1"
                     /translation="MKIFTSKMFVLAVMAGLVLGLTAWVDDPPDFHTGAPGELTCASC
                     HVPPSVNPSVGTLEIIGLPDSLQPATEYRFTVRMTKTSGDAIQAGFQMTLVNERDVKF
                     GVFSNPSPKSAISVRPYRDYFEHRTPGSFATGNVIEWNVSWTSPPADEGFSQVLRLYA
                     SGLMSNADSSITGDVTLNVNETIRYFSPYKRLEGGITGTNITCSGAKDGTATASATGG
                     KPPYKYNWNTGDTTETVDNLMAGTYSLTITDQGTDTIVKIVTLTQPFPVFGTIIKTDI
                     PCNSSVAGEAIVVPNSGIPPYSFLWSNGDTTTTTSFSKHGPISVVITDSNGCSYTAHS
                     SIIKKGDFTASISEVTNVKCFGGSTGTARVTTSIPFPTSFTWSNGASTQYVEGLAAGT
                     YSVTVTDVDGCEARASAEVLQPTAIQVKKNQSNQPSCRGSFNGYISVFADGGVPPYSF
                     AWEDGTNLKTLANLTAGDYTVTVTDAFGCTSSGTFVLTEPDEIDILVDKNNESASNAN
                     NGKALILPSGGSIPYTYLWSDGVTSRDRTGMAPGYYTVTVTDVKGCTAETGLYIEPFE
                     CGLVVTGVEVHDVECNGTAGGYIHLEVTGGTEPYNFKWSNGADVQNLNQVKSGKYTLT
                     ITDAAGCQSGGFFSINQPPPFKKTLTVTDATAADQFDGKVKFVFSGGVPPYTLIYDVT
                     DTLTTYTGIVTFENQLAGLHSIYVEDANGCVQSEFFIINIIGCQLEPSNVVIQDVKCN
                     GGSDGAICVGVSGATGAYDLYWDDETTGPCLLELEAGMYSLYITDEAGCQSLDTFTIR
                     QPSILREINSHVIKPDPGLNNGSIAVFNTGGIPDYTYTWYKDSVRIDGNGSLISGLGT
                     GIYYYELQDANGCGFVSDTFDLFVPTSSKHSITDSSLKLYPNPTNGSIYLDFGLSESS
                     GVRVFNATGMQVAVRVIRNQYRPEIDLDKLAPGVYTIIVDHEKGRSVGRIVLMR"
     gene            8694..9788
                     /locus_tag="PLG24_02545"
     CDS             8694..9788
                     /locus_tag="PLG24_02545"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HMZ23793.1"
                     /translation="MKFLKILDWFIIKKYLTTFFFTAFIFTMIAVVIDTSDKLEKFLR
                     EECTLQEIIFQYYGAFIPHINWLLWPLFSLISVIFFTSRMAANSEVISILNAGVSYNR
                     FLRPYFISAGFICLVHAIGNHFFIPILNKNRIDFEAQYIKKVDNQNQNNEVHLMLSAD
                     SKVFVRFYNRADSTARDLRMETFKNGNLVEIYKAREAKFLGNPNQWLLTGLERHTFDS
                     LQETIRIQPRDTVRMSLNIGPDDFYTIRNFKETLPSPALLRFIKKEESRGTGVAGEYR
                     IEFHRRTADSATLIILMLIGVSVASRKVRGGMGMNLAFGVSLGAAYMILSRFSVTFAQ
                     SNIISPLLGVWIPNLIFIVVSIWLVSKAQK"
     gene            9945..11708
                     /locus_tag="PLG24_02550"
     CDS             9945..11708
                     /locus_tag="PLG24_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ23794.1"
                     /translation="MNKILSCLLVLLCIYSYGQTIPQWRIADWTGVGIKKPMNDDRVL
                     LRIADFGGLGNGTTSNVIAWDEAISALQGKPGIIEFDQGIYLFDRIISIPSHTILRGK
                     GAESTILRFVYPATGNLISLQGRLDDDVAFLTRDAFRNDDFIILTTTDAFNAGDLVRI
                     SMDDTGLVTSDWAKGTVGQIIEIKDQRHDTLFLSEQLRLDLPLQRQPVIRKIDPVKYA
                     GIECLKIVRGSNDAFQSNNISLEFASNCLINGVESDSANLAHIAVSSSSHVEVRNCYL
                     HDSYDYGGGGKGYGILVQATSGDCLIENNIFKHLRHSMILQAGANGNVFGYNYSIEPY
                     WTGTTLPSNASGDLVLHGNYVFSNLFEGNIGQQIVIDDSHGINGPFNTFFRNRLESYG
                     IFMNNNPPSDSQNLVGNEVTSTVFTQGLYLLFGKDHFQYGNNIRGSITPVGTEELSDT
                     SYYLTKKPPFLDDISQYPIIGTPNVFKSGKNSAAFNFTNGIFTRCGDDVISGNQSISE
                     REVSVALYPNPTRGNFIINGLKPGLGIRLYDIMGKLIFQQEVVNACINIDLSGFNNGL
                     YFVNIMAAGGIVQTLKIVKMN"
BASE COUNT         3390 a         2637 c         2406 g         3581 t
ORIGIN      
        1 caccccgaaa ggcagaacta ctaagcattg tggctattaa tcatctacct acttcagaag
       61 tggcatcccc tccatccccc cccaaatcga agtaggtagg aaagtaataa ttcaaaaacc
      121 tccatacaca aggatttgga ggcaaatgaa aaatttaact cccgggtaaa tcatctgaat
      181 tttggcaagg ctgccccgaa aggtaatacc tgcaagcatt gtggctatta atcatctacc
      241 tacttcagag accgcacctc caccatcctc ccaaaaatcg aagtaggtag gaaagtaata
      301 actcaaaaac cgccatacac aaggatttgg aggcaaatga aaaatttaat tcccgagcaa
      361 atcaactgaa ttttggcaaa gctaccccga aaggcagtac cttcaagcat tgtggctatt
      421 aatcatctac ctacttcaga tgctccattg ttctcaaccc ccactataag ctattaccct
      481 ttgcgttgaa ctgccaagct catctatcga catcctgaca acatcccctg accttaagaa
      541 taccggttct ggcttgatac ccgcacctac accctcaggg gtgccggtac acaaaatatc
      601 acccggaaga agtgtcatga atctgctgat gtacgataca aggtaaggaa tgtcgaatat
      661 catatcagct gtactgccct gttgcctgat gatctcattt acgcttaacg taagagaaag
      721 attatggaca tcctctactt catcctttgt tgctaaaaat gggcccattg gagcaaaggt
      781 atcacaactt tttcccttgc agaattggcc tcccatctct ttttggaacg cacgctcact
      841 gtaatcatta attaggctat atccggcgat atagtcatat gcttcttccg gttcaatata
      901 cgaggcacgt cgtcccatga taaccgccaa ctcgatttcc caatccgtct taacactgtc
      961 acgtggtatc attacatcgt cattcggccc actcaaagca gtggttgact tgaaaaataa
     1021 aataggttga gtgggtatgt tggcattggt ctccatgaca tgattcttgt agttgagacc
     1081 tatacatact attttggatg gtcttgcaac cggagatcca agccttgtat cctgcggaat
     1141 ctccgccata gttgttgttg atgaatgaat tatttgctcc agtctttgca gaccatcatt
     1201 ctcaaaaaat gtttcatcat agtcatgaat gtaagaggaa acatcccggt actttccatc
     1261 caataatatt cccggctttt ctttgccttt ttgcccgaag cgaataagct tcatatcgtt
     1321 tataatttta ttttcattga ataagatttg gatcgatcac gtagagataa tatgacgtga
     1381 tgtttgatgt ccatcgatcg taaagataaa gtgctattgg caaacctgat ttgaaattca
     1441 caaaaaaaag aggccggtat taccggcctc ttttttgatg cttaataact atttctattg
     1501 aaattattct tttcctgcag cacttctcaa agcacgtgga atctcaatgc ttacaacagg
     1561 aatacttccg ttctgcataa cttcaattcc atcttgagca atgatatcct tcaccctcaa
     1621 tgattgtccc agttccaatc cggaaatatc gacaaataat tctcctacca atttttccgg
     1681 aactgtcttt acctttatct tacgaacact ttggatcaac ttaccaccga cttttactcc
     1741 gggactgctt ccttttgttt taagcggaat ctcaacttta acgggattgc cctctgtcaa
     1801 ctccaggaaa tcaatgtgcg taatggtatc ttttaccgga tgaaactgga tatcctttaa
     1861 aatagccttg gattttgcac cgtcaacatt caactcggca actttgaatt caggtgtgaa
     1921 tacaagatgt cgaacttcag cctcttgagt ggtgaagtga gttgtcccat tttttccgta
     1981 aagtacacaa ggaattcctt ctgaagatcg aacagccttt gaggcctttt ttcctacttc
     2041 atttctctta tttccgttga tagctatagt attcattgtc gtatatttaa atgccctttt
     2101 taatatcgga gtgcaaaggt aaaattgttt tacaaattga caaaataatt ctttaaaaat
     2161 ctgttgatta tgtccgatga cagaattgga tagttgtaac gaagtaggat aatgtaatgg
     2221 tggattaaaa aatcattgac ttcttgccat aacgattgaa ccattaccaa tcgaagatga
     2281 atatttcaat atatttgcaa ctcaaataag attaccttaa agaccagtcc aggtccggtt
     2341 tatttactca aattttaata tccatggtta ttatcccgcc ccatattgaa aatttagccc
     2401 catataaacc cggaaagcct ggtgctaata tgtttgaaga tgccaagccg gagcgtgaag
     2461 ttgtgctgag cagcaatgag aataatttcg ggccaagccc aaaagcgatg gaagccttga
     2521 aaaggtcgct taataacatg aatttgtacc cggacccgac tgcatcctta ctcaaggaac
     2581 gcctgtccca cctgttggat atacctgtga gccacctact gatgagcaat ggttcggacg
     2641 ctatccttta cactatgttc agggcttttt tgtcaggaga tgacagcatg ctcacttctc
     2701 atgccgcctt tgtatccgcc aaagtgatgg ctcgtatgaa caatatacgt tgtgtggaag
     2761 ttgctatgac cggagatcat gagtttgatc tggatggtat aatcacaaac attgatgata
     2821 ctactaagtt gatttatttg gtcaacccaa ataatccgac tggtcagatg atgacgcgga
     2881 cagctatgga ttcattccta agccgtgttc ctcaacacat tttggtcgta gtcgatgaag
     2941 cctattgcga gtttgctcgg gcgctttccg gagaataccc ggactgtacc cgattgggat
     3001 ataataatgt cattgtgttg aggacttttt caaaagctta cggcttagcg ggtttacggt
     3061 tgggttatgc aattggtcct gaatatttga tccaggctct tactaaagtc aaattgacct
     3121 tcgatcccaa ccttgcagca caggccgccg gattagccgc tttagatgac acgacattct
     3181 tagacaaaac cattgagaac aataccacag aattacagaa acttaaccaa gcttatgacc
     3241 gcctgggatt gaaagtcata ccctccgtag ccaatttcat taccatcaaa ctaaagaatg
     3301 aagccgaagt ggaaaactat tatcatcagt tattatctcg tggagtattg acaagacgct
     3361 tagcatcatt tggtctacct tattgtctta ggatttccat tggcaagcct gatgagaatg
     3421 actacatgtt acggatgttg gaagaggtta tggaccaata attctttttg ttcagccagg
     3481 agctctaatt gattaagaaa agtaaaaaga atctaaacct gggtttcaac tcttttcccg
     3541 gagtgcttca cttaccgcaa ggtcaaaaaa ttgattaagc gtccagccat gggctattgc
     3601 ctgctgtgga ataatgcttg cttcggatag ccccgggacg gtgttgacct ctatgagatg
     3661 gaatgtgtcc cctaccaaaa tataatctac ccttacaaag cctgaacaat ttagtgctgt
     3721 atatattttg ctagtatatt ctttaacttg tcgttcaagt tcggatgaaa tctctgcagg
     3781 agtgacctct cttgatgctc cttcatattt ggcttcaaaa tcaaagaact ccttcagtgg
     3841 gatgatttca gtcaccggaa gcacgacaat ctctccattg atcctcatga ctccattccc
     3901 aaattcacgt cccgccatca tagactctac catcacgtcc tgatcgaact ggaaagcata
     3961 ttctatcgcg ggaattaact cttcaatgtt ctttaccttc ctaactccaa aactggaacc
     4021 atgtgcattg ggttttacaa atagaggaag tcccagcttt gcaatgatgt cttctgtatc
     4081 cacgccctca cgatatgctg cttttgtcac caaaacttcc ctggccatag gtatatcata
     4141 ggcttgaagt gttcttttag taagggattt attcatggta agtcccgaaa caaaaccatc
     4201 acaacaagta taaggtattc caaggatgtc aaaataaccc tggataatgc cattctccat
     4261 gggcgcacca tggatggcac aaaagacaac ttcaggcttc catttcgacc cgcccagatc
     4321 caaggaaaaa tcattcttat caattgaatg cccggtatca agatctatcc attcacgccc
     4381 attgatatct atgatgcgcg catcatattt ttcaccatga agatatttat ggacgactcc
     4441 ggcgctcttg acggatacta cccgctcttc agtatctcca cctgcgagaa tcgctacttt
     4501 gatcttggtt gacataataa ttttatttaa tgtgtaaaat tatataattc tgctggaatt
     4561 aataaattat actaagatga tttatataat agcccataag tttttagtat acttgtccgg
     4621 atagctatgc tactaaagct ctgctctgtc ccgggagtga aacaatccgg ggttccggga
     4681 tgccggggtt acagtcgtat aggccaattt cattgatcta tacgactgta ccttcggtat
     4741 tactttgaag aaatatccac taatttgcat acatccatca ggtagaatct gattgcagtc
     4801 tgaatggatt gaaagtggaa gcccggcaaa atctgaatcg tattcaaaaa tcgaagtatg
     4861 tatgaaaata atatcctcaa aacgcgaact actcgggaaa cccgaaagtc aatttctata
     4921 aattgattcg ctaagtaatc atttccggcc tgtctatccc aaagttaccg actcttctat
     4981 gtttcatcta ttaaacatgt acctaataca ggttgaattg cccgacttct gtcctactta
     5041 ctcaaccctg atatcattca tgccatattt cctcccgcca attcaaaata agattccggt
     5101 aataccgatt gtggtttgtt aatttttatt tcaattctgt tcagtgaaat gcttcgcaat
     5161 atcaagaacc ggaagcattt gcctgaacct tttggcttac cggaatttta taggaataga
     5221 tggactgcct gttctgattt attatttggg gcccatcttt ccccgtgttc tatacggagt
     5281 gtgatatgaa ttcacttgcc caattatcat acccaacaca gacagcaacg catgcattaa
     5341 aaaaaaggga taattcatag gactatataa tctttagcat attatcccca tttattttaa
     5401 attgtcaatt aacgcataag tacaattctg ccaacactgc gacctttttc atgatctaca
     5461 atgattgtat aaactcccgg tgctaatttg tccaaatcta tctccgggcg gtattgattg
     5521 cgaattactc tgacagctac ctgcattcct gttgcattga atacgcgaac tccggaactt
     5581 tccgataagc cgaaatccag atagattgag ccgtttgttg ggttaggata caactttaac
     5641 gatgagtctg taatagaatg ctttgaactg gtaggtacga agagatcgaa agtatccgat
     5701 acaaatccac atccattggc atcctgcaat tcataataat aaataccggt tccaagccct
     5761 gatattaatg aaccgttacc gtctatacgg acactgtctt tataccaggt ataagtataa
     5821 tcaggaatcc caccggtgtt gaaaacagca atagatccat tgttcagtcc gggatccggt
     5881 ttgatgacat ggctgttgat ttccctcaaa atggaaggct gtcttattgt aaatgtatcc
     5941 agactttggc aacctgcttc atcagtgata tacaggctat acatgccggc ttctagctca
     6001 agcaggcatg gaccggtggt ttcgtcatcc cagtacaggt cataagcacc tgtggcaccg
     6061 ctgacaccaa cacaaattgc accatcgctt cctccgttac attttacatc ctggatcact
     6121 acattggatg gctccagctg gcagccaatt atattgatga taaaaaattc actttgaaca
     6181 caaccattgg catcttcaac ataaatacta tgcagccctg ccaactgatt ctcaaatgtt
     6241 actataccgg tataggtagt caaagtatcc gttacatcat agatcaatgt ataaggaggc
     6301 actcctccgg aaaagacaaa ctttaccttc ccgtcaaact gatcggctgc tgttgcatct
     6361 gtcactgtca atgttttttt aaatggcggg ggttggttga tactgaaaaa ccctccggac
     6421 tgacagcccg ccgcatctgt tattgtgagt gtatatttac cggatttgac ctgattcaga
     6481 ttttgaacat ccgctccatt ggaccactta aaattataag gctctgtacc acctgtcacc
     6541 tcaagatgaa tatatccacc cgcggtgcca ttacactcga catcgtgtac ctcaactccg
     6601 gtcaccacca acccacactc aaatggttca atgtatagac cggtttctgc tgtacaccct
     6661 ttgacatcgg tgacagttac tgtatagtaa cccggagcca taccggtccg atcacgggaa
     6721 gttacaccat ctgaccatag gtaggtatat gggatggagc cgccggaagg taggatcaac
     6781 gcctttccat tatttgcatt ggaggcactt tcattgtttt tatccactaa aatatcaatt
     6841 tcatccggtt ccgtcaacac aaaggttccg gaagaggtgc agccgaaagc atcggtcacc
     6901 gtaacagtat aatctccggc cgtaagattg gcaagcgtct tgagatttgt tccatcctcc
     6961 caggcaaatg aatacggagg tacgcctcca tctgcaaata ccgaaatata accattaaaa
     7021 ctgccacgac aactgggctg gttggattgg ttctttttaa cctgaattgc cgtcggctgt
     7081 agtacttcag ctgaggctct ggcttcacat ccatcaacat ctgttactgt cacactgtac
     7141 gtccctgctg caagaccctc gacatattga gtcgatgcac cattagacca ggtaaatgaa
     7201 gtagggaacg ggatagacgt ggttacgcga gctgttcctg tggatcctcc aaaacatttc
     7261 acattggtta cctctgaaat ggaggcggta aaatcgcctt tcttgatgat tgagctgtga
     7321 gctgtgtagc tgcaaccatt ggaatcggtt ataaccacag aaatcgggcc atgttttgaa
     7381 aatgatgtgg tagttgtagt atctccatta gaccaaagga aactataagg tggtattccg
     7441 gaattcggta ccacgattgc ttctcctgca acggaagaat tacagggtat gtcggtcttt
     7501 atgatggtcc cgaacacagg aaaaggttga gttaaagtca ctattttgac gatggtatca
     7561 gttccttgat cggtgatggt gaggctatag gtgcctgcca taagattatc gacagtttct
     7621 gttgtatctc ccgtattcca gttgtatttg tagggtggtt ttccacctgt ggcactggcg
     7681 gtagctgttc catcttttgc tccactgcaa gtaatattgg ttccggtgat gcccccttct
     7741 agccttttat atggagaaaa atagcgaatt gtttcattga cgttcaaggt tacatctcct
     7801 gttatactgc tgtcggcatt gctcatcaga ccactggcat aaagtcgcaa tacctggcta
     7861 aatccttcat cagccggagg gcttgtccag gaaacattcc actcaatgac attacccgta
     7921 gcaaatgatc cgggcgtcct gtgttcgaag taatcgcgat acggacgcac agaaattgca
     7981 gattttggtg atggattgct gaatactccg aattttacat ccctttcgtt gaccagagtc
     8041 atctggaatc ccgcctggat tgcatcccca ctggttttcg tcattctaac cgtgaagcga
     8101 tattcagtgg cgggctgcaa tgaatcggga agacctataa tctcaagtgt cccaacagaa
     8161 ggattaaccg atggaggaac atggcatgaa gcacaggtca attctcccgg agctccggta
     8221 tgaaagtcag gcggatcatc aacccaggcg gttaacccca aaacaaggcc ggccatgaca
     8281 gccagaacaa acatcttact tgtaaatatc ttcataatga aggggatttt tttgttcaaa
     8341 gtaagcaaag ataatcagtc cttgataaaa ttttccaaat tgttgtcatc ctcacttcca
     8401 tcattattat caactattgg aatatgcgcc attgtaaaga ctttcgatga atgatgtaga
     8461 ataattcaat ttcatactat ttcatatagg ggaagagtca tcgatagtag cggctgaatg
     8521 cctgtttctg atttcgcctt atttatagca tcatggattg atacaacttt tttacctttg
     8581 ggcgttatct tgtcgcttca tgaatattta caattttgat tctaataaag tgtcgttgga
     8641 tgagcaagcc ggacccggag ataattttca ttgctaactg ttataactaa aggatgaagt
     8701 ttctgaaaat cctggactgg tttatcatta agaagtattt gacgaccttc ttctttacag
     8761 cgtttatctt tacaatgatt gccgttgtaa ttgacacaag tgataaactt gaaaaatttc
     8821 ttcgggaaga gtgtacactt caggagatca tcttccaata ttatggagca tttataccac
     8881 acatcaattg gcttttatgg cccttgtttt cactgatcag cgtcattttc ttcacttccc
     8941 gaatggcggc aaactctgag gtaatcagta ttctgaatgc cggagtgagc tataacaggt
     9001 ttcttcgacc gtatttcatt tcggcggggt tcatatgtct ggtacatgcg ataggcaatc
     9061 acttttttat tcctattctc aacaaaaaca ggattgactt tgaagcccaa tacatcaaga
     9121 aggttgacaa ccagaatcag aataatgaag tacacctcat gctttcagct gattccaagg
     9181 tctttgtgcg attttacaat cgggcggata gtacggccag agatttgcgc atggagactt
     9241 tcaagaatgg caacctggta gaaatctata aagcccgtga agccaaattt ctcggtaatc
     9301 caaatcaatg gctgttgaca gggctggaga ggcatacttt tgacagctta caggaaacca
     9361 tccggatcca gccacgagac acagtgagga tgagcctcaa catcggtccg gatgacttct
     9421 ataccattcg taattttaag gaaacattac cttctcctgc cttgctccga tttataaaga
     9481 aagaagaatc ccggggcacc ggagtggcag gtgagtatag gattgagttc cacagacgca
     9541 ctgccgatag tgcaactctg attatattaa tgctcatcgg tgtatccgtg gcttcccgta
     9601 aggtaagagg tgggatgggt atgaaccttg cctttggcgt ttcccttggc gctgcctata
     9661 tgatcctttc acggttttct gtaacctttg cacaaagtaa tattatttct ccacttctgg
     9721 gtgtctggat tccaaatctc atatttattg tagtaagtat ttggctggta agcaaggcac
     9781 agaagtaatg tataggttgc cgagggattt gtttttggaa gcctttgggt tattccggtt
     9841 ataatattta tcaatctttc ctccgaaaat attcaccgct aaacattttt caatcatttt
     9901 tatgctgatg tgactatatt tggatattgc atcccttttt tcgcatgaat aaaatattgt
     9961 cgtgcctgtt ggtcctattg tgcatttata gctatggcca gaccattcca cagtggcgta
    10021 tcgctgattg gacaggtgtt ggcatcaaaa aaccaatgaa tgatgatcgg gtgcttctta
    10081 gaattgcaga ctttggtggg ctgggaaatg gaaccacatc caatgtcatc gcatgggatg
    10141 aagctatatc ggccttacaa ggcaaaccgg gaatcattga attcgatcaa ggcatctatc
    10201 tgtttgaccg aatcatttct attcccagtc ataccattct gagaggcaag ggcgcggagt
    10261 ctaccatcct gcgatttgtc tatccggcaa caggcaatct tatttcactt caaggcagat
    10321 tggatgatga cgtagccttt ttgacccggg atgcatttcg aaatgatgac tttataatcc
    10381 tgacaaccac agatgctttc aatgccggtg atcttgtccg tatatccatg gatgataccg
    10441 gtctggttac ctctgattgg gctaaaggca cggttgggca gatcatagaa ataaaagacc
    10501 aacgtcacga cacgcttttt ctatccgaac agctcagatt ggatttgccg ttacaacgac
    10561 agcccgtgat tagaaagata gatccggtca agtatgcagg tatagaatgt cttaaaattg
    10621 tccgcggtag taatgatgct ttccagtcca ataacatttc tctggagttt gcatccaatt
    10681 gcctgataaa tggggtggag agcgatagtg ccaatttggc acatatagct gtcagttcga
    10741 gctctcatgt tgaagtacga aattgctatt tgcacgattc ctatgattat ggcggaggag
    10801 gaaaggggta tggcatcctg gttcaagcaa cctccggaga ttgcctgatt gaaaacaaca
    10861 tctttaagca tcttcggcat tcgatgatac tccaggcagg cgccaatggc aatgttttcg
    10921 gctataatta ttctatcgag ccttactgga ccggcacaac gcttccatca aacgcttcgg
    10981 gtgacctcgt actccatggc aactatgtgt tttccaatct ttttgaaggg aatataggcc
    11041 aacaaattgt catcgatgac tctcatggca tcaatggccc cttcaatacc ttcttcagaa
    11101 acagactaga gtcatatggt atatttatga acaacaatcc accttcagat agtcagaatt
    11161 tggtaggcaa tgaagttacc agtacggtat ttacacaagg cctgtatctg ctttttggca
    11221 aggatcattt ccagtatggc aacaatatca ggggaagcat tactccagta gggacagaag
    11281 aattgtctga tacatcctat tatttgacta aaaaaccacc tttcttggat gacatcagtc
    11341 aatatccgat aatcggcaca cccaatgtat ttaaaagcgg aaagaattcc gcagcattca
    11401 attttaccaa tggtatattt acccgttgtg gtgatgatgt gatttcagga aatcagtcca
    11461 tttccgaaag agaggtttca gtcgccttgt atcccaatcc aacccggggc aactttatca
    11521 tcaatggtct taaacccgga ctgggcatca ggttgtatga cattatgggt aaattgattt
    11581 ttcaacaaga ggtcgtcaat gcttgtataa atattgacct gtccggcttc aacaacggcc
    11641 tgtactttgt caatattatg gctgccggtg gaattgtcca aaccttgaag atagtaaaaa
    11701 tgaattaagg gtttttaagg gatgtggtaa caagaaaatt tttgaggatt cgacttcctg
    11761 tatggaatgc cgtccaatac cagatcaggc agtgacttaa cgtaatacac tgtttaaaga
    11821 gctggacttc acaatcgggt ttaaattcct ttccttctct tttgggattg tctgttagcg
    11881 aatcgacatt ctactcttag ttcaatacat caacccttaa atttcacatg attacggaag
    11941 cttctgacct gcaatttcta taatacgaaa atgaatattt aaatagacca aaatgaatct
    12001 aaagaaatat attt
//
LOCUS       DAOQQN010000041        11859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1897_length_11859_cov_5.298712, whole genome shotgun sequence.
ACCESSION   DAOQQN010000041 DAOQQN010000000
VERSION     DAOQQN010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11859
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1897_length_11859_cov_5.298712"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(583..1014)
                     /locus_tag="PLG24_02555"
     CDS             complement(583..1014)
                     /locus_tag="PLG24_02555"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HMZ23795.1"
                     /translation="MRIAIINGPNLNLIGIREKEIYGDKNFNDFLTELQQCYPNVDFL
                     YYQSNVEGELINDIQETGFLVDGIILNAGAYTHTSLAIADAVAAVKAPVIEVHISNIS
                     AREPVRHDSHLTAKCVGIISGLGLDGYRLAVEWFVEREKRK"
     gene            complement(1016..3214)
                     /locus_tag="PLG24_02560"
     CDS             complement(1016..3214)
                     /locus_tag="PLG24_02560"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HMZ23796.1"
                     /translation="MEVKEGYLTAALQKYFGFNKFKAQQKEIIESLLAGNDTFVLMPT
                     GGGKSLCYQLPALLMEGTAIIISPLIALMKNQVDSIRGYSSRDNVAHFLNSSLNKGQI
                     KQVKQDIDEGKTKLLYIAPETLTKEENLEYFHNSKISFVAVDEAHCISEWGHDFRPEY
                     RRIRTMIEAIGQHIPVIALTATATPKVRSDIIKILDMDHENTFVSSFNRDNLYYEIRP
                     KVSKEQTMKSMLQFIKSQKKISGIIYVQNRKTTEDLAEVLNVNGIKAAPYHAGLDAKS
                     RARTQDDFLMENVEVICATIAFGMGIDKPDVRFVIHYDIPKSIENYYQETGRAGRDGL
                     EGNCIAYFSVKDLTKLEKFLRDKPVAEREMGALLLQEVEAYAESSACRRKFLLHYFGE
                     EFDASDCHKMCDNCRNPKELKDVTEEMQLALEITKELNEEYNIKVLVDFLTGNATQQL
                     KLYKFTEYDSFGCGKDYDDNFWNSIFRQALLRNLMYKDIENYGLIKISETGQEFMKTP
                     TSIKIPLNQVFPEDTTPDDEGNGGTAALDETLVKLLMDVRKKESKRHNVPPFVIFTDP
                     SIEDMATQYPISIDDMAGITGVSRGKAERYGKPFIDVIAKYVEENDIERPMDFLVKQV
                     ANKSKIKVNIIQSIDKKIPLEDIARANNLDRDEFYQELYGIVTSGTKLDLNYYTEDLI
                     DEYVKEDIFDYFMNSETDNLEDASKKLKELDITWEEIELMRIVFLSEMAN"
     gene            complement(3942..4973)
                     /gene="recA"
                     /locus_tag="PLG24_02565"
     CDS             complement(3942..4973)
                     /gene="recA"
                     /locus_tag="PLG24_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HMZ23797.1"
                     /translation="MSTEKEAKLKALQLTLDKLEKTYGKGTIMKLGDKPEVDVDAIST
                     GSLGLDIALGTGGFPKGRVIEIYGPESSGKTTLAIHAIAESQKKGGTAAFIDAEHAFD
                     KSYAERLGVDTTNLYISQPDNGEQALEIAENLIRSGAIDIIVIDSVAALVPKSEIEGE
                     MGDSKMGLQARLMSQALRKLTATIGRTGCCCIFINQLREKIGVMFGNPETTTGGNALK
                     FYASQRLDIRRSGSPIKDKDGNLLGNQVKVKVVKNKLAPPFRVAVFDIMYGEGISKSG
                     EIVDLGVDLDIIQKSGSWYAYENTKIAQGRDAAKQFIEDNPGVAEEIEAKIKDKIVAK
                     GGTASIEMD"
     gene            6053..7399
                     /gene="mtaB"
                     /locus_tag="PLG24_02570"
     CDS             6053..7399
                     /gene="mtaB"
                     /locus_tag="PLG24_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HMZ23798.1"
                     /translation="MATPRSVAFYTLGCKLNYSESSSISRMFENNGYSVNEFHEGADI
                     FVINTCSVTDSADKKCRNIVRQALRISPQAKVVVIGCYAQLKPEEITRIEGVDLVLGA
                     AEKFRIIEFVESIGNAPSKGMMMAGDVREANQFVSSFSMGDRTRSFLKVQDGCDYKCS
                     FCTIPMARGASRSDTTAQVIENARYIASQGIKEIVLTGVNLGDFGNGTEVIEGISHKK
                     NELFIDLIKKLDEVEGIDRFRISSIEPNLLTNEIIEFVAQSKRFVPHFHIPLQSGNNK
                     ILAAMRRRYKCELYADRVRYIKELMPHCCIGVDVIVGFPGETDEDFWETVRFIQNLDI
                     SYLHVFTYSERANTPAAEMGQVPVHIRRQRNDVLGQLSEVKRKLFYERHQGETRSMLV
                     EHGHSNMELTGYTDNYIKVSLPYDETLINQVVSVELGAFDVAGNLVGHIVSSEIIV"
     gene            7402..8103
                     /locus_tag="PLG24_02575"
     CDS             7402..8103
                     /locus_tag="PLG24_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HMZ23799.1"
                     /translation="MCGRASLTKNESDLEESFGASFYTEDIARYIPLPAFNIGPVQKL
                     PFIAQDDPLHFQIGWWGWNVTIGNNTQLVINARLEEIDKKRTFVPHLNHGRCIIPLDG
                     YYEWQKCGSQKIPYRITVPDGGLFAVAGLWREEVANDGTVSKRIVIITRAAQKDIAGI
                     HDRMPLILNKEEWVEWMDASFTDKMKAIFSDCERMITLVHYKVSSRVNSIKNNDSSLI
                     EPDDSPIYIQKSLFD"
     gene            8123..8920
                     /locus_tag="PLG24_02580"
     CDS             8123..8920
                     /locus_tag="PLG24_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ23800.1"
                     /translation="MKSIRTTLCMLLVTLFTFQSDAQGGLYLGLNGSGNATIVANQMN
                     YGQKEMDYDAVTFRPAFGLELGYDLNGGSMLSIGANLQQAGQKYKDGYGNGDKLNKEI
                     KMSYFAIPVTYRLVFGGKKDPNHGTKFFVAVGPQISLLQSAEVTHQVNEKEVTLETFA
                     NHIAGGGAPYNFNSAEIGKLTTNGEPANDKDLFASMDIEGFVGLGIQSFLSESFFMTL
                     ELRGGGSLTDINAKDWRLDGYKDGQRQKYEASRNVFAGLRLGLNYKF"
     gene            8994..9638
                     /locus_tag="PLG24_02585"
     CDS             8994..9638
                     /locus_tag="PLG24_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23801.1"
                     /translation="MAKVMARILSFFTHPLWMITFGLFIYLKLDPYAFGVYDWTLRIP
                     FVITVFMMTFFFPALVIFLLLKLDFIKSIELHTARERIIPYIATSIFYLWLYITTRSN
                     PEIAALFKIFVLGTVITLFLIFFINNFSKASAHMAGLAGLLILTIALAYRLGPNDGLG
                     WLTSRPSNLPSMVVLIAGLTGSARLKLEAHTWQELLGGFFIGLLGQLIALKIYF"
     gene            9709..10170
                     /gene="smpB"
                     /locus_tag="PLG24_02590"
     CDS             9709..10170
                     /gene="smpB"
                     /locus_tag="PLG24_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HMZ23802.1"
                     /translation="MAEKFKNIEIKNKRASFEYFLLQKFEAGLMLKGTEVKAIRLGSA
                     NLTDAYCYIRKGEVFVKNLFISEYSQGSYYNHDARRERKLLLNKTEIRKIERRLSEKG
                     FTLVPTRLYMNERGFAKLEVALAQGKKMYDKRASIKEKDNRRDMDRFKVSR"
     gene            complement(10581..11102)
                     /locus_tag="PLG24_02595"
     CDS             complement(10581..11102)
                     /locus_tag="PLG24_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23803.1"
                     /translation="MKYIFVFFTFFTTLSGGYSQVITDIKPDTTTLKEFEQRIKLTRI
                     DGKYIPKDLNDAMMELNKIMEDGAKKKFAEMSENDARQKTHFSFGKYINARWYIQEGS
                     RLTIWFQKNKIYDFDDMIDCIIVSYHRTLNNKPIEFEILADYYYKKQMEKVKKLVEDK
                     KKREVRVKLNEKE"
     gene            11250..>11859
                     /locus_tag="PLG24_02600"
     CDS             11250..>11859
                     /locus_tag="PLG24_02600"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HMZ23804.1"
                     /translation="MKHLGTILSGISLLILIILFNSKWGSIPPLGKLLSPTHGYLIDP
                     ELQGIQSGFSMVELGGLKDSVQVYFDDRMVPHIFASNDEDAYFVQGYLHARDRLWQME
                     FQTHFAAGRLSEIVGEKALVVDRTNRRLGMVYAAEKSLEEMEKDTLTFLAMNAYTAGV
                     NSYIGTLEEKDLPLEYKILDYQPEPWSNLKSALLSKYMAKDLA"
BASE COUNT         3335 a         2189 c         2509 g         3826 t
ORIGIN      
        1 atattggtaa gccaggccat tttgcgtatt tgggttttca agataagaaa atccacatcc
       61 tcattgcggg cctagatatt aagctatgaa tatacttctg gataagcccg aaacacagag
      121 aatgattaat tttttatgtc ttgtagaagt tttaccttgg ttttagacct ccaaatcggc
      181 taaaattgtg gattactttc gccggaatat tcagttattg ggaaatttaa aaatactatt
      241 caagtttcaa gggtgccttg atgctggtaa atagtaaaaa accagaaggt catcttctat
      301 tttgtttaaa tatgagatac gtatcttggt tttttagttt gaatatcaat tttgcaaagt
      361 ctcggtttac aaattttgaa aatatggtgg ggtgtcttta attcagctag aaaactttaa
      421 attgtttgta cctcaatatc cattggctta taaagcagga ttaagttagc aagaaatgag
      481 atttttttta ctttgaatag attttcaaag ttaaaaaaaa tgaaacttac ataatcaaat
      541 cgactgatta tactattttg gaaaaaataa aaaaggcaaa atttattttc ttttttctct
      601 ctccacaaac cattcaactg ccagccggta accatccagt cccagtccgc tgatgatgcc
      661 tacacatttt gccgtgagat gtgaatcatg acgcaccggt tcacgtgctg atatgttgga
      721 aatatgtacc tcgatgactg gagccttcac cgctgccact gcgtcggcaa tggcgaggga
      781 ggtatgtgta taagcacctg catttaggat tatgccatct acaagaaagc ctgtctcctg
      841 gatgtcattg atgagttcgc cttcgacatt ggactgataa tagaggaaat ctacattggg
      901 gtagcattgt tgcaattcag tcagaaaatc attaaaattc ttgtctccat aaatttcctt
      961 ttcccggata ccgatcaggt tgagattcgg tccgttgatg atggctatac gcatatcaat
     1021 tggccatttc tgaaaggaag acaatacgca tcagctctat ttcctcccag gttatgtcaa
     1081 gctctttcag ttttttggag gcatcttcca ggttgtcagt ttcgctgttc atgaaataat
     1141 caaagatatc ttccttgacg tattcatcga tgagatcttc ggtataataa ttcaggtcta
     1201 gcttggtccc ggaggttaca atgccataca attcctggta gaactcgtcc cggtcgaggt
     1261 tgttggcacg agcgatatct tccagcggta ttttcttatc tatgctttgg atgatgttta
     1321 cttttatctt ggatttgtta gccacctgct tgacaaggaa gtccatcggt ctttcgatgt
     1381 cattttcttc gacgtatttg gctatcacat caataaatgg cttgccgtat cgctcagcct
     1441 tacctcggct gacaccggtt atcccggcca tatcgtcaat agatatcgga tattgtgttg
     1501 ccatatcttc gatcgaaggg tcagtaaata tcacgaaggg aggtacatta tgacgtttgg
     1561 attctttctt tctgacatcc atcagcagct tgaccagggt ttcatccaaa gctgcagttc
     1621 ctccgttgcc ttcatcatca ggggttgtat cttccggaaa tacctgattc agtggaattt
     1681 ttatcgaagt tggcgttttc atgaattcct ggcctgtttc ggaaattttt atcaaaccgt
     1741 aattctcaat atccttgtac attaggttgc gcaataaggc ttgcctgaag atggagttcc
     1801 agaaattgtc gtcataatcc tttccgcaac caaatgaatc gtactctgta aatttgtata
     1861 atttgagttg ttgggtagcg tttcccgtga ggaagtctac taaaaccttg atattatatt
     1921 cctcgtttag ctctttggta atctcaagtg ccagctgcat ttcttcggta acatccttga
     1981 gttccttagg attccgacag ttgtcacaca ttttgtgaca gtcggaagca tcaaactctt
     2041 cgccaaaata gtgcaggaga aatttacgtc ggcaggcgga actttctgcg taagcttcta
     2101 cctcctgaag caagagtgcc cccatttccc tttctgcaac cggtttgtct cttaggaatt
     2161 tttccagctt tgtcaggtcc ttgacagaaa aataagcgat gcagtttcct tccagcccat
     2221 ccctgcctgc tcgtccggtt tcctgatagt aattttcaat gcttttgggt atgtcataat
     2281 gaatgacgaa tcgtacatcc ggtttgtcaa tccccatacc gaaagcgatg gttgcacaga
     2341 ttacttccac attttccatc aggaagtcat cctgagtgcg ggcgcgtgat ttggcatcca
     2401 ggccggcgtg atatggggcc gccttgatac cgttgacatt gagcacttca gcaaggtctt
     2461 cagtggtctt ccggttttga acataaataa tacctgaaat ctttttttga gacttgatga
     2521 actgcagcat gcttttcatc gtttgctcct tagatacctt gggccttatt tcatagtaca
     2581 agttgtctct gttaaaagaa gatacgaagg tattctcatg atccatatcg aggatcttga
     2641 taatatccga acgaacttta ggagttgcgg tagctgtcag ggcgatgacg gggatgtgtt
     2701 ggccgatggc ctctatcata gttctgatac ggcggtattc aggtcggaaa tcatgtcccc
     2761 attcggagat acagtgtgcc tcatcaacag cgacgaagga tatttttgaa ttatggaagt
     2821 attctaaatt ttcttctttg gtgagagtct ccggagcgat gtataagagt tttgttttgc
     2881 cttcatcgat atcctgtttg acctgtttga tctgaccttt attcagtgaa ctattgagaa
     2941 aatgagcgac attatcccgg ctgctataac ccctaatgct gtccacctga tttttcatca
     3001 gagctatcaa tggtgatatg atgatggctg ttccttccat taataaggca ggaagctgat
     3061 agcaaagaga tttaccgcct ccggtaggca tgaggacaaa agtgtcattg cctgccagca
     3121 gactttctat gatttccttc tgttgtgcct tgaatttatt gaaaccgaag tacttctgta
     3181 atgctgcggt taaataacct tctttaactt ccataattca ttacggatat gctattttaa
     3241 taaataaaat ctatgatcac tgtttgactt aatcaaaatg ccttttagaa aaagaaacaa
     3301 aatcattctg ttttgcctca aatttgaatt ccatcttaat tctttccagt taccaatttt
     3361 tatttgagat tatcaaacca agataaggat ttttttaatt taaaaagcaa ataattttat
     3421 gagaatttta accttggatt aattagattt tcatttaatc gtacattgac atgcaacacg
     3481 ttgttttatt gatttgtata aatatatatt cagattatat tatccgaaga taacccaaca
     3541 aatcgccttg ggatgatggt agtaaatgca catttataaa tatatgagat tgtgaaggat
     3601 tgggtcattt gatatgtatt attggttgat tctccgataa attgtattaa gcattgacaa
     3661 tcaattggaa ggtatcggct ggaaatttgg caataagctg agcaaatcat caaccctaat
     3721 gcatcagttc ggaataagtg ataccaggat gatttatata aaagtcctta aatttttggt
     3781 atagtgccga ggttacagtc gtacagatca atgaatttgg tctataacga atgtaccttc
     3841 ggtgttaaat aatggtatta aacaaatgaa tggaagtaaa aaaaaacagg cggttggagg
     3901 tgatatctca accgcctgca aatgttgttt tcaggggatt tttaatccat ctcaatggat
     3961 gcagtaccgc ccttagctac gatcttgtcc ttgattttag cttctatttc ttcagccact
     4021 cccgggttat cctcaataaa ctgcttagca gcatctcgtc cctgggctat tttggtattt
     4081 tcgtaagcat accagcttcc tgatttctgt atgatatcca gatccactcc caggtcaact
     4141 atttcacctg atttggagat gccttcgccg tacattatat cgaagactgc tacacggaaa
     4201 ggcggtgcca atttgttttt gacgactttg acttttacct ggttgccgag gaggtttcca
     4261 tctttatctt ttattggaga gccacttctt cggatatcca gacgttggga ggcatagaac
     4321 ttaagagcgt ttcctccggt tgtcgtttcc ggattgccaa acataacacc tatcttttca
     4381 cggagttggt tgataaagat acagcaacac cctgttctgc ctatcgtagc agtaagtttt
     4441 ctaagagctt gagacatcag gcgtgcctgg agccccatct tggaatcacc catctcacct
     4501 tcaatttcac ttttaggtac cagggctgca actgagtcga tgacaatgat gtctattgca
     4561 cctgaccgaa taagattttc tgctatttca agcgcttgtt caccattgtc aggttgcgaa
     4621 atgtacaggt tggttgtatc tactcccagg cgttcggcat atgatttgtc gaaggcatgt
     4681 tcagcatcta tgaaggctgc cgtaccgcct tttttctgac tttcggcaat ggcatgtatg
     4741 gcgagtgttg tcttaccgga agattcaggt ccataaattt ctataacacg acctttagga
     4801 aatcctcccg tacccagggc gatatcgagg ccaagagaac ctgtggagat ggcatctaca
     4861 tcgacttcag gcttgtctcc aagcttcatg atggtgcctt tgccataagt tttttcgagc
     4921 ttgtccaggg ttaactgaag ggcttttaat ttcgcttctt tttctgttga cataagattt
     4981 tgatttaaaa aattaaagaa tgatttgaaa tcccgggtga gttaaacatc ctaaaaaatg
     5041 attaatcccc aagaatctgc aaatatattg aaataataat aaattataat ctattaaatg
     5101 tttatttttt cgtcattttt tgacgaaaaa atcagaatgc tgcagaaatt tacttttctg
     5161 atatagttat ttatcgatcc gagtattgcc ggagatggtc gtaccacaat atttggttga
     5221 tgacttattt tagtcaatgc atataatttt gcttttattg ggctattatg aatcgagtat
     5281 agagattttg aaattttctt caagcttgaa atccggtgtg tcattttatt tagttgccaa
     5341 gtaagttggt ataaaaaggt tggaatgagg gggcttggga agtaggtagg aaaataataa
     5401 tcatcagtca agccaatgaa gggtctattt tataatgtat gattattttg ttttcattat
     5461 cggtattaaa ttcaattcag gtggttaaag tatccgtgaa tattgattgg atcactatta
     5521 atcttctacc tacttagaaa ttgcataaca aaactatttt ttgctcttaa cccaaggagc
     5581 tttttgattt aaatgctgaa ttgaagaggg atgatttctt aaaaattcga aataggtagt
     5641 tgtttaatag tcgtctattc agttattatc aataatgatg agatttcatt ctggaaaatt
     5701 ttgattgaaa gccgataatt tgaataggca tactgtcctt gcatgtatca cgtccgattt
     5761 catagttatt aatcatctac ctacttgaat tttagatata aataccaatc cggtcgtcaa
     5821 tcctttacat gtattgtcac agttgcttga aggccccgct tttaacgggt gaggcgtgtt
     5881 cttttaagca aatgctttat ttgtgcccgg aatcagcata gttttccaat caaatggaaa
     5941 aatttagggt gctgccgatt tgctttatgg ccgaggtgtc aatggttagc caccggaata
     6001 accattctgt gccaaaaaaa atcactacct ttgcagcctt aaatagaata ttatggctac
     6061 tcctagaagt gttgcattct atacattggg ttgtaagctc aattattccg agtcctcatc
     6121 gatttccagg atgtttgaaa acaatggtta ttcggtcaat gaatttcatg aaggagcgga
     6181 tatttttgtg atcaatactt gtagcgtgac ggattctgct gataaaaaat gcaggaatat
     6241 tgttcgccag gcgctgcgta tttctccaca ggcgaaggtg gttgtaatcg gttgctatgc
     6301 gcaattaaaa cctgaagaaa tcacccggat agaaggggtg gacctggttt tgggtgctgc
     6361 tgaaaaattc cggattattg aattcgtcga gagtataggc aatgcccctt ctaaaggaat
     6421 gatgatggcc ggtgatgtgc gcgaggccaa ccagtttgtc tcttccttct ccatgggtga
     6481 tcgtactcgt tcttttctga aggtgcaaga tggatgtgac tataaatgca gcttttgtac
     6541 cataccgatg gctcgtgggg caagcagaag cgatactacg gctcaggtca tcgaaaatgc
     6601 cagatatata gcatcccagg gcattaagga aattgtcttg acgggggtga atcttggcga
     6661 tttcggtaat ggaacagagg tgattgaagg tatttcgcat aagaagaatg agctgttcat
     6721 tgatctgatt aagaagctgg atgaagtgga aggtattgac agattcagga tctcctccat
     6781 agagccgaac cttcttacta atgaaatcat cgaattcgtg gcacagagta agcgctttgt
     6841 tcctcacttc cacataccac ttcagtcagg taataataag atccttgctg caatgcgtcg
     6901 caggtataaa tgtgaattat atgctgaccg tgtcagatat attaaggaac tgatgcctca
     6961 ctgctgcatt ggtgtggatg tcattgtcgg cttccccgga gagactgatg aagatttttg
     7021 ggagacggtg cgttttattc aaaacctgga tatcagctac cttcatgttt tcacatattc
     7081 tgaaagagcc aatacgccgg ccgcagagat gggacaggtg cctgtacata tcaggcgaca
     7141 aaggaatgat gtgcttggac aattgtctga ggttaagcgt aaattgttct acgagaggca
     7201 tcagggtgaa acaaggtcta tgctggttga gcatgggcac tcaaatatgg aactaaccgg
     7261 ttatacggat aattacatta aggtatcgct gccatatgac gaaacattga tcaatcaggt
     7321 tgtctctgta gaattggggg catttgatgt tgccggcaat cttgtaggac atattgtatc
     7381 cagtgaaatt atagtctgac catgtgtgga agagcatcac taaccaaaaa tgaaagtgac
     7441 ctggaggaga gctttggcgc aagcttctat acggaagaca tagcgaggta tattccattg
     7501 ccggccttca atataggtcc tgtacagaag ttgcctttca ttgctcagga tgatccactg
     7561 cattttcaga tcggttggtg gggatggaat gttacaatcg gcaacaatac ccagttggta
     7621 attaatgctc gcctggagga aatcgataaa aagaggacat ttgttccaca tctcaatcat
     7681 gggcgttgca tcatacccct tgacgggtat tatgaatggc aaaaatgtgg aagtcagaaa
     7741 attccctacc gaattacagt gccggatggc gggttgtttg cagtggcagg actgtggcgg
     7801 gaggaagttg ctaatgatgg tacggtctca aaacgcatcg tcatcatcac tcgagcagct
     7861 cagaaagata ttgccggtat tcatgatcgg atgcccttaa tcctgaataa agaggaatgg
     7921 gtggaatgga tggatgctag ttttaccgat aaaatgaagg caattttttc tgattgcgaa
     7981 agaatgatta ccttggttca ttataaggtg tcatccaggg tcaattctat caaaaataac
     8041 gattcatcat tgatagaacc ggatgattcg ccgatatata ttcagaaaag cttatttgat
     8101 taacttacaa aaaatacaaa caatgaaatc aatcagaaca acactttgca tgttacttgt
     8161 tacgttattt acctttcaat ctgatgctca gggaggttta tatcttgggc taaatggaag
     8221 tggaaatgcc accatcgttg ccaatcagat gaattatggg caaaaggaaa tggattatga
     8281 tgcagtcacc ttccgccctg catttggcct ggagctggga tatgacctga atggaggcag
     8341 tatgttgtct attggcgcca atctgcaaca ggccggccag aaatacaagg acggttatgg
     8401 aaatggcgac aagctaaata aagaaatcaa gatgagttat tttgctattc ccgttacata
     8461 tcgcctggtc tttgggggga aaaaggatcc taatcatgga acgaaatttt ttgttgccgt
     8521 tggtccacag atttctcttt tacaaagtgc tgaggttact caccaagtaa atgagaagga
     8581 ggttacactt gagacatttg caaatcatat agccggtgga ggtgccccct ataattttaa
     8641 ttcagccgaa atcggcaaat tgacaaccaa tggtgaaccc gccaatgaca aggacctgtt
     8701 tgcttctatg gatattgaag gttttgtagg cttgggaata cagagttttc tatcagagtc
     8761 attttttatg acacttgaat tgcgcggcgg aggttcattg accgatatca atgccaagga
     8821 ttggaggttg gatgggtata aggatggtca aagacagaaa tatgaagcga gtcgtaatgt
     8881 ttttgctgga ctgagattag gacttaacta taaattctag gtataggttt caaggaagat
     8941 cccgaatcga tcggaatatt tcaagaattt ataaacacca aattatcaag gaattggcaa
     9001 aggtgatggc ccggattttg tcctttttta ctcatcctct ttggatgatc actttcggat
     9061 tgtttattta tcttaaactg gatccatatg ccttcggtgt ctatgactgg acacttcgta
     9121 ttccctttgt tatcacagtg tttatgatga catttttctt tccggcgctg gtcatttttt
     9181 tgttgctaaa gctggatttt atcaaaagca tagaattaca tacagccagg gaacgtatca
     9241 ttccgtatat tgccacttca attttttatt tgtggctata catcaccaca cggagtaatc
     9301 ctgagatagc ggctcttttc aagatctttg tgcttggcac agtcattaca ttattcctta
     9361 tatttttcat taataatttt tcaaaggcaa gtgcacatat ggcaggtctg gcgggtttgc
     9421 tcatattgac cattgcattg gcatacaggc ttgggcctaa tgatggtttg gggtggttga
     9481 catcaaggcc gtccaatctg ccatccatgg ttgttttgat agccgggttg acaggaagtg
     9541 cgaggttgaa gctggaggct cacacctggc aagaattgtt gggtggtttt tttataggcc
     9601 tgttaggtca attgatcgcc ttaaaaattt atttctgacc attcgaacaa taatattcat
     9661 tacgggttac actctacaat atagcatttc gtgtaacttt gcattcctat ggctgaaaaa
     9721 ttcaagaata ttgaaataaa gaacaaaagg gcttcctttg aatatttttt gcttcagaag
     9781 tttgaagccg gccttatgct gaaaggtaca gaagtaaagg ccatacgctt aggttctgct
     9841 aatcttactg atgcatattg ctatatccgt aagggcgaag tatttgtaaa gaatttgttc
     9901 atttcagagt atagtcaggg ctcatactac aaccatgatg ctcggcgtga aagaaaacta
     9961 ttactcaata agactgaaat cagaaagatt gagcgtcgcc tttcggaaaa aggatttact
    10021 ttggtcccga ccaggcttta catgaatgag cgaggatttg ccaaattgga ggtagcattg
    10081 gcccaaggta aaaaaatgta tgacaaacgg gcaagtatca aggaaaagga caatcgacgg
    10141 gatatggata ggtttaaggt aagtcgttga tggatctcgg cttctgcatt ctgccgtaaa
    10201 tgcctgatta gtagttgcat gtaatttgct cgtaatatac ttctgactat taatatcaaa
    10261 accaaatact tacattccaa atagtaattc cattattagc ttacaattaa tttaaagact
    10321 cctgcttcct ttgatttata ggttgaccta ttcctcctgc ctgaatatag tctcaacaaa
    10381 ttctaccgga tcaatatgtt ggagtcgttt gatacaaaat ttaaattatt gaagtcaaat
    10441 tgagattgag actcaacagc tcttatttct gctcaaactt gtaaaactaa acgggcgaaa
    10501 acccttcaat caagcattaa acgttgttac ataaaacttt ttgaccaaag tttcaagata
    10561 atcaaatcac aggactttag ttattccttt tcattgagtt ttaccctaac ttcccttttc
    10621 tttttatctt caaccagttt tttgactttt tccatttgct ttttatagta ataatcggca
    10681 agtatttcga attcaatggg tttattatta agggtacgat ggtaggagac gataatacaa
    10741 tcaatcatgt cgtcgaagtc ataaattttg ttcttttgaa accaaatggt caacctgctt
    10801 ccttcctgga tgtaccagcg tgcgttgata tatttaccga atgagaaatg cgtcttttgt
    10861 ctggcatcat tttctgacat ttcggcaaat tttttcttag ctccgtcttc catgatttta
    10921 tttaattcca tcatggcatc attcaaatct tttggaatgt acttgccatc gattcgggta
    10981 agttttattc tttgctcaaa ttccttcaaa gtggttgtat caggttttat atctgtaatg
    11041 acctgagaat agcctccact aagagtagtg aaaaatgtga aaaaaacaaa aatgtatttc
    11101 atggttttct cttaaccttt caaacgccgc aaacataaaa taaattaccc ttatttgtaa
    11161 caaattgagt tattaaaggg gaaaatttta aatttgtcaa atcaatcgtc caggttgagt
    11221 gaagtttttt aaaatattta tgtaataaaa tgaagcattt gggcaccatc ctttccggaa
    11281 tttcactatt aattctgatt atcctgttca attcaaaatg gggctccatt cctcctctgg
    11341 gcaagttgtt aagtcctacg cacggatacc tgatagatcc tgaacttcag ggtattcaat
    11401 ccggattttc tatggtggag cttggtggac taaaggattc agtgcaagtc tattttgatg
    11461 accgtatggt acctcatatt ttcgcttcca atgatgaaga tgcttatttt gttcaaggat
    11521 atcttcatgc cagagatcgt ctatggcaaa tggagtttca gacgcatttt gctgccggtc
    11581 gattgagtga aatagttggg gaaaaggcat tggttgtaga tcgcactaac aggaggctgg
    11641 gcatggttta tgcagccgag aagagtctgg aagagatgga gaaggatacc cttacttttc
    11701 tggcaatgaa tgcttataca gccggtgtaa atagttatat aggcacattg gaagaaaaag
    11761 atttgccttt ggaatataaa attctggatt atcaaccgga accatggtcc aatttgaagt
    11821 cagcattatt aagcaaatat atggcaaaag accttgcag
//
LOCUS       DAOQQN010000042        11785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1918_length_11785_cov_6.141773, whole genome shotgun sequence.
ACCESSION   DAOQQN010000042 DAOQQN010000000
VERSION     DAOQQN010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11785
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1918_length_11785_cov_6.141773"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2188
                     /locus_tag="PLG24_02605"
     CDS             <1..2188
                     /locus_tag="PLG24_02605"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HMZ23805.1"
                     /translation="VEGENNSLVAISVFEDNIIGMVSTEASGNIVISQTENRGVYLLY
                     SDRDLKMQNPTSCAAVEPNHYAEEIAQLLNNQLQTRDEKCFKLYLECDYALYQNKGSV
                     SAVTDWVTALYNNVAALYQNESLLTKISEIYVWTTPDPYSKTSSYTALTQFKTQRPNF
                     NGDLAQLVALGGNNIGGIAWIGGLCTAYKYSYANIQNYYNSVPVYSWTVEVMTHEIGH
                     NLGSPHTHSCSWPGGALDNCYPVEGNCNPGPPPTTGGTIMSYCHLTNYGINFNNGFGQ
                     KPGDLIRAKVNASSCLGYCDDGGGPMCGAPQSVTISNITNNSAMVSWTTGLNGIYYIV
                     EYKVSTSNTWTILPQQSSTSLLLSGLNSGTTYHVRIKTICSEGESEYSAVYTFTTGNA
                     CGIPYGPFVQNITETSAYAYWKPVPGATSYDFRFKLTSSNNWATINTSSTSISLSGLT
                     PGTSYDVGVRARCGTVYSDYTGTYTFVTLGNPPGGGSGYCDSHGTSSAFDWIQKLTFG
                     SFTNESGNNNGYGDFLNQTISAKKNTMVIISVTPGMIGNYTEFWTVWIDFNQNESFTD
                     PGEQVATFTTTNTNPNYTKIFIPDNASLGNTRMRIQMKRGSFSTSCEAFPYGEVEDYT
                     LSITQQLKGGTSEYSSSNNTTLFPNPFNENFTLNFESENAEDARIEISDITSRIIFSH
                     PMSVQKGLNSFRLEDTSSMEPGIYMVRIIGQNINETHKAIKVSNRN"
     gene            complement(2536..3060)
                     /locus_tag="PLG24_02610"
     CDS             complement(2536..3060)
                     /locus_tag="PLG24_02610"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HMZ23806.1"
                     /translation="MIVSAIVAVAENGVIGDRGAMSWHLKSDFKNFKKKTLHHPIIMG
                     RTTFESIGRPLPKRENIIITRDMFYLASGALIAHSVEEAMDLAARTGNEEVFIIGGAE
                     IFRQTIGLWDKLYYTEVHMVARGDTFFPMINWDEWVLTERIYQPKEENDDSDCTFKVF
                     LRSGRFDSHGISDY"
     gene            complement(3057..4919)
                     /gene="mrdA"
                     /locus_tag="PLG24_02615"
     CDS             complement(3057..4919)
                     /gene="mrdA"
                     /locus_tag="PLG24_02615"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HMZ23807.1"
                     /translation="MVAKPDSRIRIIQMVFIMSAMVLVMKAAQIQLFDTSFQVRADAT
                     AIEKHVVYPARGIIYDRNGKLLVNNDPMYDLMVTYNAIDPNMDTAKFCNILGIDKETF
                     IANLSKDWASKRFSKSIPFPFLKKISPYTYTRFQESLWEFPGFFPQLRIARGYPHHNA
                     ANVLGYIREVDQNQIASSGGLYGLGDYIGISGMEGQYERLLMGRKGIQYIMKDNMGRP
                     VGPYRNGAQDSIPVSGQDLISSIDLDLQQYAESIMQGKRGSVVAIEPQTGEILAAVSS
                     PSYDPNLLTINQGRGKAFGELINDYNKPLYNRIAQAKYPPGSIFKTVVALSAMQMGVL
                     TPNRSISCHGAYYYRGGKVKCHAHPASVNVSHALQYSCNTYFITVLRDAVDKYGFTRP
                     SKGLTELDRHLFDFGLGHPLGTDMMHETKGNIPTPAYYEKIYGKDWRSTYMMFIGIGQ
                     GEIQLSALQIANLAAVIANRGYYYVPHLIKGFKNKEMEIPEQFREKHMSGVDAKFFKP
                     VIDGMDAAVASKWCGWSTPIPGITLCGKTGTSQNPPYRDHAVFMAFAPKDNPKIAIAV
                     LIENAGFGAQSAAPIASLIVEKYLRGYIEAPFRKEREKEMTRLNLLGPPKLDKK"
     gene            complement(4919..5440)
                     /locus_tag="PLG24_02620"
     CDS             complement(4919..5440)
                     /locus_tag="PLG24_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23808.1"
                     /translation="MGGTIARNLVRFVFILLLQVLVFRSLTIYYPVLDNISIIIYPVI
                     IMLLPMRTPGALILVIAFVLGILVDMFYNTPGVHASACVFTGFIRPFVLSRLEPRGGY
                     TVDSSPTIIAYGNRWFLFYSSILLLLHLFFLFSVQKFTFVYFLEIITRTFLSFIASMI
                     FILVYMYLFNPKE"
     gene            complement(5446..6273)
                     /gene="mreC"
                     /locus_tag="PLG24_02625"
     CDS             complement(5446..6273)
                     /gene="mreC"
                     /locus_tag="PLG24_02625"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HMZ23809.1"
                     /translation="MQNLFQFFARYGSAILFIALEVLCFALIVRYNSGQKEIFINSGN
                     FYSGVLLENYSKITHFYRLSGIADSMANENARLRAELRFATIDASFRHDSLIDTVYRQ
                     RYSFISAQVVSNSVNQIDNFITINKGSDFGVKPGMGVINNAGVVGIVRQTSRNYATVA
                     SLLGSQTKISAAIKRNGYFGSLVWDNTSTEYVYLTDIPKHADILKGDSVITSGYSTTF
                     PRGIYIGRVENVGFDPGSSFYDLKVKLTTDFNNLTYVYVILDKTKPQLDSLNNAILR"
     gene            complement(6302..7333)
                     /locus_tag="PLG24_02630"
     CDS             complement(6302..7333)
                     /locus_tag="PLG24_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HMZ23810.1"
                     /translation="MKLFKFFTQELAIDLGTANTLIIQDDKIVVDEPSIVAINRKTGE
                     TIAVGNRAMQMHEKTHENIKTIRPLKDGVIADFQAAESMIEGLIKMIENRRTFFGHLK
                     MVICIPSGITEVEKRAVFDSADHVDSKETYLIHEPMAAALGIGLDVQEPIGNMVIDIG
                     GGTTEIAVIALSGIVCDQSIRIAGDEFSSDIISYVKRQHNIAIGERTAELIKIQVGSA
                     LQVLDNPPDDCFVNGRDLMTGIPKQISVSYQEIAHALDKSISKIEEAVIRALETTPPE
                     LASDIYKTGLYLTGGGALLRGLDKRIAQKTKLNVHVADDPLRAVVRGTGIALKNTDSF
                     SFLIDRKSI"
     gene            8052..8852
                     /locus_tag="PLG24_02635"
     CDS             8052..8852
                     /locus_tag="PLG24_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23811.1"
                     /translation="MKKNLFLLLTTLISFTLLESCTLYRAVHYESVDIGFFKNKNEGN
                     ATFEYDGNNRTGKLGGNISYAVGNNFFVGIGGGIHNHELQKVLAPLSEYNFDENDFTL
                     SGYNGKGFVGYFNNFGRGLNSYFETVLTLNASSETLKINDLNPNITTKTVFNYKPIGF
                     GLQIGIGINTDKVGFLGGVKLNSYSFNTSLPLTYHSSILEYRSTKSLTQIFAGVRFGS
                     GPVKGNIQLSVGHNLYPWYIDDFNEIVPVFGFGVTYNWGRKKTKIINL"
     gene            9491..10375
                     /locus_tag="PLG24_02640"
     CDS             9491..10375
                     /locus_tag="PLG24_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HMZ23812.1"
                     /translation="MSILKVGLVQQTCSTNRDENIRKSIAGIRECANKGAELIVLQEL
                     HTGIYFCQVEDPDIFDLAESIPGPSTDIFSRVAKECGIVLVTSLFERRAPGIYHNTAV
                     VFDKDGTMAGKYRKMHIPDDPAYYEKFYFTPGDLGFEPIQTSVGKLGVLVCWDQWYPE
                     AARMMALGGAEILIYPTAIGWESSDNPEEKQRQHDAWQTVQRGHAIANGLTVITVNRV
                     GHEVDWSNVTGGIQFWGQSFVAGPQGELLHHGSSTQEENIVIDINLKRNEKVRRIWPF
                     LRDRRIDAYNDLLKRYRD"
     gene            complement(10410..11420)
                     /locus_tag="PLG24_02645"
     CDS             complement(10410..11420)
                     /locus_tag="PLG24_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23813.1"
                     /translation="MPKLIIPFLSLYLFSIFSCQLPDQEKVGRFDDNTILAEISNDTL
                     EKSINSTSIPIDSLWRRYAYDSNWIKMPEVLPGSILPHYRIIAFYGNLYSKQMGILGE
                     LPGSEMIALLNNEIEAWHDADSLTPLMPALHVIATTAQRLPGADKMYRLRMNRSMIHT
                     VKSMADSIGALTFIDLQVGHSNLQREIPVWDSLLNLPTVHLGIDAEFAMYNKKVPGTV
                     MGRFDAADINYAIDYLEEKVKSHNLPPKILIVHRFKRGMITNALKIKQTPLVQVVMHM
                     DGWGAKELKFHSYRAYIQREPVQYTGFKLFYRNDTKFGKSLLTPKEILELNPKPLYIQ
                     YQ"
BASE COUNT         3669 a         2419 c         2330 g         3367 t
ORIGIN      
        1 tgtcgagggt gaaaataatt ctctggtggc aattagtgtc tttgaagata acatcatcgg
       61 aatggtatcc acagaagcaa gtggaaatat cgtcatatca caaaccgaga atcgaggtgt
      121 atatttgtta tattccgacc gcgatctaaa aatgcagaat ccgacatcct gcgctgctgt
      181 tgagcctaat cattatgcgg aagaaatagc acaactgctt aataaccaat tgcagaccag
      241 ggatgaaaaa tgttttaaac tatatcttga atgcgactat gccctctacc aaaacaaagg
      301 atcagtatca gctgtaacag attgggtaac agccttatat aacaatgtcg ccgctttata
      361 ccagaatgaa agtcttctga ctaaaatttc tgaaatatat gtctggacaa caccggaccc
      421 ctacagtaaa acaagcagtt atactgctct gactcaattc aagacacaac gtccaaactt
      481 caatggggac ctggctcaac ttgtagccct tggaggaaat aatattggag gtatagcgtg
      541 gataggcgga ctatgtacgg catataaata tagctatgcc aatatccaga actactacaa
      601 ttccgtaccg gtttattcat ggacagttga agtaatgacg catgagatcg gccataacct
      661 tggttctcca catacccata gttgttcatg gccaggaggt gcactggaca attgctaccc
      721 tgtggaaggg aattgtaatc cgggcccgcc accgaccact ggtggaacca tcatgagtta
      781 ctgtcacctg acaaattatg gcatcaattt caacaatggt tttgggcaaa agccaggtga
      841 cctgattcgt gccaaggtca atgccagttc atgtcttggg tattgtgatg atgggggggg
      901 acctatgtgc ggagctccgc aaagtgtgac tatttccaac atcaccaaca attctgccat
      961 ggtatcctgg acgacgggac tcaacggcat ttactatatc gtggaataca aggtgtctac
     1021 ctccaatacc tggactattc tacctcaaca aagttctaca tcattacttt tatcaggatt
     1081 aaattcaggc acaacctacc atgtaaggat aaaaaccatc tgcagtgaag gtgaatccga
     1141 atactccgct gtatatacct ttacaacagg aaatgcatgt ggaattccct atgggccttt
     1201 cgtgcaaaat atcacagaaa ccagtgccta tgcatattgg aaaccggttc ccggggccac
     1261 ttcctatgat ttcaggttta aattgacgtc cagcaacaat tgggctacca tcaatacatc
     1321 ttcaacaagc atttcccttt caggattaac gccgggtact tcatacgatg taggtgtcag
     1381 ggcgagatgc ggtactgtgt acagtgacta taccgggacc tatacattcg taacccttgg
     1441 taatcctccc ggtggcgggt ccggttattg tgactctcat ggaacaagct ctgcttttga
     1501 ctggattcaa aaattaactt tcggttcatt tacaaatgaa agcggtaata acaacggata
     1561 tggcgacttt ttgaatcaaa ccataagtgc aaagaaaaat acgatggtaa ttatttcagt
     1621 taccccgggt atgatcggta actataccga attctggact gtttggatcg actttaacca
     1681 gaatgaaagc tttaccgatc cgggagaaca agtagcaaca tttaccacaa caaataccaa
     1741 tccgaattac actaaaatct ttataccgga taatgcttcc ttaggaaata caagaatgag
     1801 aatccaaatg aagagaggat ctttttcgac ttcctgcgaa gcattccctt atggcgaggt
     1861 agaagattac acgctcagta taacccagca attgaaagga ggcacctcag agtacagttc
     1921 atcaaataat acgaccctct tccccaaccc attcaacgag aactttactt tgaatttcga
     1981 atcagagaat gccgaagatg ccagaatcga aataagcgat atcaccagca gaatcatatt
     2041 ctcgcatcct atgagtgtcc aaaaaggatt gaacagcttc cgtttagaag atacttcctc
     2101 catggaaccc ggaatataca tggtacgtat tataggacaa aatatcaatg aaactcacaa
     2161 agcaataaaa gtttctaaca gaaactaaaa tttttaagtt aagatgttta gttggcgggc
     2221 cttcagggcc tgcctttttt atataagaca aatcaaaaac gcgcaaccag caagtataat
     2281 tcaactcaag ggtaataaga gatctatcac cccccataag tattgaactc ttcatgtacc
     2341 aggcttcaaa ccatactgtt tgggttcaga gcaatctgtg cagtcatttg catcgctatt
     2401 aataaagctt tcatttagta aaaaggaagc gtatccaatg aacgtcccga aaaaatcaaa
     2461 gattatccaa gagctaataa ctcaatcatc gacatgtatc cgctccgaca ggatatttca
     2521 tccaaaatag aatggtcaat agtcggaaat accatgagag tcaaagcgac ctgatcgcag
     2581 gaagaccttg aaggtgcaat cactgtcatc attttcttct tttggttgat agatacgttc
     2641 tgtcagaacc cattcgtccc agttgatcat aggaaaaaaa gtatctcccc tggccaccat
     2701 atgcacttcg gtataataca gtttgtccca caaccctatg gtctgcctga atatttctgc
     2761 tccaccgatg ataaacactt cttcattacc ggtgcgagct gccagatcca tcgcttcttc
     2821 cacggaatgt gcaatcaaag ctcctgaggc gagataaaac atatctcggg ttatgataat
     2881 attctctctt ttcggcaagg ggcgtccgat tgactcaaag gtggtacgcc ccattatgat
     2941 cggatggtgt agtgtttttt tcttaaagtt tttaaagtca gatttcagat gccaggacat
     3001 ggctcctcta tcaccgatga cgccgttttc tgcaacggct acaatagctg aaacgatcat
     3061 tttttatcta attttggtgg cccaaggaga ttaagccgtg tcatttcctt ttctctttcc
     3121 ttcctgaatg gagcttcaat gtagccccga agatatttct ccacgatcaa tgaagcgatt
     3181 ggtgcagcag actgagctcc gaacccggca ttttctatca acacagcaat ggcaatcttc
     3241 ggattatcct ttggtgcgaa tgccatgaaa acagcgtgat ctctataggg tggattttga
     3301 gaagtccctg tcttaccaca aagcgtgata ccgggtatag gtgtgctcca gccgcaccac
     3361 ttactggcta cagccgcatc catgccatca attacgggct tgaaaaactt cgcatccaca
     3421 cctgacatgt gcttttcgcg aaactgctca gggatctcca tttccttatt tttaaagcct
     3481 ttgattaaat ggggaacata atagtaaccc cgattggcaa taacagctgc aagatttgca
     3541 atctgcaaag ctgaaagttg tatctcaccc tgaccgatcc caataaacat catataggta
     3601 gatctccagt ctttgccata tattttttca taataagcag gtgtcggaat attgcctttt
     3661 gtctcatgca tcatgtcggt accaagcggg tgacccaatc caaagtcaaa cagatgtcga
     3721 tcgagctctg taagaccttt tgaaggtctc gtgaacccgt acttgtcaac ggcatcccta
     3781 agtacagtga taaaataggt gttacatgaa tactggagtg catgtgagac attgacagaa
     3841 gccgggtgag catgacattt gactttacct cccctataat agtaagcgcc atggcagctg
     3901 attgaacgat tcggtgtaag gacacccatc tgcattgccg aaagagctac aacagtctta
     3961 aatatcgagc cgggaggata cttagcttga gcgatcctgt tgtacaatgg tttgttataa
     4021 tcattaatca actcaccaaa agctttgccc cgaccttgat taatagtaag cagattaggg
     4081 tcataagagg gagaactgac cgctgcaaga atttcgcctg tctgaggctc aatcgctacc
     4141 actgaccctc ttttaccttg catgatgctt tctgcatact gttgcaagtc taaatcgatg
     4201 gaagatatca ggtcctgacc tgagaccggg atggaatctt gtgcgccatt acgatatggg
     4261 cctacgggtc tacccatatt atccttcatg atatactgga tgccttttct acccatcagc
     4321 aaacgttcat attggccttc catccccgaa atgccgatat agtccccaag cccgtaaagg
     4381 cctccgctac ttgctatctg attctggtca acctcccgga tataacctaa aacgttggca
     4441 gcattatgat gaggatagcc tcttgcgata cggagttgtg gaaaaaatcc aggaaattcc
     4501 catagagatt cctgaaatcg ggtataggta tacggtgata tttttttaag gaaaggaaaa
     4561 ggaatagact ttgaaaatct tttgctcgcc caatcttttg atagattggc aataaaagtc
     4621 tctttgtcta ttccaagaat attgcagaat ttggccgtat ccatatttgg gtcaatggca
     4681 ttataggtaa ccatcaagtc atacatcgga tcgttattga ccaaaagctt gccgtttctg
     4741 tcatagatga ttcctctggc aggataaaca acatgttttt caatggcggt cgcatcagcc
     4801 ctaacttgaa atgaagtgtc aaacaactgg atctgcgcag ctttcatcac aagcaccatg
     4861 gcactcatga taaataccat ttgaatgata cgaatcctac tatccggttt ggcgaccatt
     4921 attccttcgg attaaaaaga tacatataaa ccagaataaa aatcatagaa gcaataaagc
     4981 ttaaaaatgt ccttgttatt atttccagga agtaaacaaa tgtaaatttc tgaacggaaa
     5041 aaaggaaaaa caaatgcaga agtaataaaa tggaggaata gaacaaaaac caacgattgc
     5101 cataagcaat gatggttgga gaagaatcaa cggtatatcc tccacggggc tctaaccgtg
     5161 acaaaacaaa cggcctgata aaccctgtaa acacacatgc tgatgcatgg acacccggcg
     5221 tattataaaa catatccaca agtataccca atacaaaggc aatcaccaaa atcagggcac
     5281 ccggagtcct catcggcaat aacattatga tgaccgggta aatgataatg ctaatattgt
     5341 ccagaacagg atagtaaatt gtcagactcc tgaataccaa tacctgtaac agcaatataa
     5401 aaacaaaacg gacaaggttt cttgctattg ttcctcccat ggtcactatc ttaatattgc
     5461 attattcaac gagtcaagct ggggcttggt tttatcaaga ataacgtata cataggtcag
     5521 attgttaaag tcagtggtca acttcacctt caaatcataa aaactgctcc ccggatcaaa
     5581 accgacattt tccacccgac ctatataaat accacgtgga aatgtagtac tgtagccact
     5641 ggttatcacg ctgtcgccct tcaggatatc ggcatgctta ggaatatcag tcagatagac
     5701 gtactcggtg gaagtgttgt cccagaccaa agaaccgaag tagccatttc gtttgattgc
     5761 ggcactaatt ttggtctggc tacccaacaa ggaggcgaca gtagcataat ttcgggaagt
     5821 ctggcgaaca attcccacca caccggcatt attgataact cccattccag gtttaactcc
     5881 aaaatcagac cctttgttga tcgtgataaa gttatcgatt tggttgacag agttactaac
     5941 tacctgggca gaaataaaag aatatctctg acggtatact gtgtcaatca aggaatcatg
     6001 tcgaaatgat gcatctatcg tagcgaacct tagttcagcc ctcaacctgg cattttcatt
     6061 ggccatactg tctgcaatgc cagaaaggcg ataaaaatgg gtaattttgc tataattttc
     6121 aagcaataca cctgaataga aatttccaga attgataaag atttcctttt gaccgctgtt
     6181 gtatctgaca atgagggcaa agcacaaaac ttccagggca ataaaaagaa ttgctgatcc
     6241 gtatcgggca aaaaactgaa ataaattttg catatgtcag tgtgctttag cccgccggtc
     6301 tttatatgct ttttctgtca atcaggaaag agaaactatc cgtattcttc aaggcgatgc
     6361 cggtacctct gacaacagct ctcagagggt catccgcaac atgtacatta agcttggttt
     6421 tttgtgcaat ccttttatca agccctctca acaatgctcc accgccggtg aggtacaatc
     6481 ctgtcttata tatatccgat gcgagttccg gaggcgtggt ttccagtgcc cggattacag
     6541 cctcttcaat cttggagatg gacttgtcca gcgcatgagc aatttcctga taggaaacag
     6601 atatttgctt cggaattccg gtcatgaggt ccctgccatt gacaaaacag tcatcagggg
     6661 gattgtccag tacttgcaga gccgagccaa cctgaatctt tatcagttct gccgttcgtt
     6721 ctccgattgc gatattgtgc tgtcgtttca catagctaat aatatcggaa gaaaactcat
     6781 ccccggcaat ccgaattgat tgatcgcaga caatacctga tagagcaatt acggcaattt
     6841 ccgtcgtgcc tccgccaatg tcaatcacca tattgcctat tggctcctga acatccaggc
     6901 caattcccaa agcagcagcc atcggttcat gaatcagata ggtttctttt gagtcaacat
     6961 gatcagctga atcaaaaact gcacgttttt ctacttcagt gattccggat ggtatacaaa
     7021 tgaccatttt caaatgtccg aaaaaggttc ttcgattctc aatcatttta atcaagcctt
     7081 cgatcatact ttcagctgcc tgaaagtcgg ctattacccc atctttcaat ggcctgattg
     7141 tcttgatgtt ctcatgagtc ttttcgtgca tttgcattgc acggttgccg actgcaatgg
     7201 tctcaccggt cttcctattg atagcgacaa tggatggctc atccacgact attttgtcat
     7261 cctgtataat gagtgtattg gctgtaccaa ggtcgatggc caattcttgt gtgaaaaact
     7321 tgaataattt catctaatca acttgatggt tcgttttaga ggggacaaaa gtacggctaa
     7381 ttgtttaatt ctcaaaaatt tattacaacc atataaaagt tacaataatc agttgattat
     7441 taatatttga acagttatat ctcaattaaa tgtcccttaa aaaagtcagg gccagtgaag
     7501 gggcaaacaa atcgacccgg caggaattca taatgctaat caaaatgaca gattcaatag
     7561 gtttagagaa ttcaactaca tctggtctta aaaatgaaaa caaatgcgtt atcccataac
     7621 attataaaat tatgatttgt aaaaaaataa tcattgggct actatgacct tcagctgcat
     7681 aatggtcaaa atggaattaa aaaaacagaa acccaataaa acctctacat tcccatctgt
     7741 taaataaatt aagttaatgt cgatctgaac cttattgcac taacattttc aaaacaaggt
     7801 caaataaact ccaaggcgca tatgatttgc cgaaacaatt gatatgattg atttctgtag
     7861 taaaatagaa tcttaaggct ttaggaaacc tgaacaaaaa aacatccgaa atccatcatc
     7921 cttctgcaat aatccattag ttaaaatatc attaaataaa tctgagacaa gataaataaa
     7981 tcattagcat cactaccttc aaggcatgta attatgtaat tttatcgtca aatcttaaaa
     8041 cattttaggt tatgaaaaag aatctgtttc tcctgctgac aactttaatt tcctttactc
     8101 ttcttgaaag ttgtacatta taccgagctg tgcattatga gagcgtcgat attggttttt
     8161 ttaaaaataa aaatgaaggc aatgctactt ttgaatatga tggcaataac agaacaggca
     8221 aattaggagg aaatatttca tacgctgttg ggaacaattt ttttgtaggt atcggaggcg
     8281 gaatacacaa tcatgaacta cagaaagtcc ttgctccgtt atcagaatac aattttgatg
     8341 aaaatgattt tacactatcc ggatacaatg gaaagggttt tgtcggatat ttcaacaatt
     8401 tcggccgtgg gctcaatagc tattttgaaa cagtcctcac tttaaatgca agctctgaga
     8461 ctttgaagat caacgatctg aatcctaata tcacgaccaa aacagtattt aactataagc
     8521 cgattggatt cgggttacag attgggatag gaatcaacac agataaagtc ggatttttag
     8581 gaggggtcaa gctaaattca tacagtttta acacgagtct accacttacc tatcattctt
     8641 cgatattaga gtatcgttcg acaaaatcac ttacacagat ttttgcaggg gtcaggtttg
     8701 gtagtgggcc ggtcaaaggt aacatacaat tgtcggttgg ccacaatttg tatccttggt
     8761 atattgatga cttcaacgaa atagttccgg tattcggctt tggtgtaact tacaattggg
     8821 gaagaaaaaa aacaaaaata atcaacctat aatcaataac ccgtgcaatt attatcgacc
     8881 tataaatctt attgattgct taatctatac tattgaccaa ccgggttatt tgccagaaag
     8941 ttttcaaatt ttatgtaata atttcttggg cttccataat tacgattatg gcgaaaattg
     9001 ctaaacgcct gatattctgg cgtataaaat ggctgtatgg ctggccttgt taagtggggg
     9061 ggtgagttcg tgaagtaggt agataattaa tagtgatgga ggcaataact gtgcgatgct
     9121 cacactaaaa ggatgaatat ttgggaaaaa ttgaacgact ttatcaattc actttctcga
     9181 ttcccttatt ataacatagt gttcaattat taatttccta cacacttcct tttcgatggt
     9241 cgcgtcataa atttgaccta ttccttttga aaagacagga agtgtaactc aggccctcta
     9301 tatttagtaa atagcatagc ttttgaatat atgcaataac tattccctaa aagcttagtc
     9361 caaggttatg ttttcgcaga actaaaattt cacaaaattt gttgactatc caatcatggt
     9421 cgttttgtaa tttcgttttt catttggtag aactgagtat tttataccaa ttaatataat
     9481 cataaaaaag atgtcaatac ttaaagtcgg gctggtacag caaacatgct ccaccaaccg
     9541 agatgagaat atcaggaaga gtatcgccgg aatacgcgaa tgcgctaaca agggggccga
     9601 actaatagtt cttcaggagc tacatacagg aatatatttt tgtcaagtag aagatcctga
     9661 tatttttgat ctggcagagt ctattccggg tccgtcaacg gatatcttca gtagggttgc
     9721 aaaggaatgt ggaatagtat tggttacttc tttgtttgaa cgcagagcgc ccggtatata
     9781 tcacaatacc gcagtagttt ttgacaaaga tggcacaatg gcaggcaagt atcgaaaaat
     9841 gcacattcct gacgatccgg cctattatga aaaattttat tttacaccgg gggacctggg
     9901 atttgaacct atacagacaa gtgtcggaaa actgggtgtc ttggtgtgct gggaccaatg
     9961 gtatccggaa gctgcgcgta tgatggcatt agggggagca gaaatcctca tttatccaac
    10021 tgcaatagga tgggaaagtt ctgataatcc tgaagaaaag caacgtcaac acgatgcctg
    10081 gcaaactgtc caacgaggtc atgccatagc taatggactg acagtcatca cggtcaatcg
    10141 ggtaggacat gaagttgact ggagtaatgt tacaggagga atacaattct ggggacaaag
    10201 ctttgttgcc ggcccacagg gggaattact ccatcatggt tcatctaccc aggaagagaa
    10261 cattgtgatt gatattaatt tgaagagaaa tgaaaaggta cgcaggatat ggccatttct
    10321 cagggatcga agaattgacg catataatga tttgctcaaa agatatcgcg attaatcaat
    10381 tgtgtgaacg tgtactctga ttctattcat tattggtatt gaatgtaaag aggctttgga
    10441 ttcagttcca gaatctcttt tggtgttaac aggctttttc cgaatttagt atcattacga
    10501 taaaacagct tgaatccagt atattgcaca ggctctcgtt gaatatatgc acgataactg
    10561 tgaaatttaa gctccttagc accccatcca tccatatgca taactacctg aactaatggg
    10621 gtttgtttta tttttaaagc gttggtaatc atgcctcgct taaatctgtg tactatgaga
    10681 attttgggag gtaagttatg acttttgact ttctcttcga gataatcaat ggcatagttg
    10741 atatcggctg catcgaagcg acccattact gttcccggaa ccttcttgtt gtacatagca
    10801 aattcagcat caatacccaa atgtactgta ggtaagttta ggaggctgtc ccataccggt
    10861 atttccctct gtaagttact atggcctact tgcaggtcga tgaaagtcaa agcacctatt
    10921 gaatctgcca tagatttgac ggtgtgaatc atagagcgat tcattcgaag acgatacatc
    10981 ttatcagccc ccggcagtcg ctgggcagtt gtagcgatta catgtaaagc gggcatcagg
    11041 ggagtcagag aatccgcatc atgccaagct tcaatttcat tatttagtaa agcaatcatc
    11101 tcagacccag gcagttcccc aagaattccc atctgcttgc tataaaggtt tccataaaac
    11161 gcaatgattc tataatgtgg taaaatggag cctggaagca cttcgggcat tttaatccaa
    11221 ttactgtcat aagcatatcg acgccataat gaatcaatag gtatggaagt tgaattaata
    11281 gatttttcaa gtgtatcgtt tgatatctca gccaggattg tattatcatc aaagcgcccg
    11341 actttttcct gatccggtaa ttgacaggaa aatatgctaa aaagatatag agaaaggaag
    11401 ggaattatca attttggcat gtaacaatca aaaaatttaa agcaaattat taaaaaaaat
    11461 atttatgtat aaataatatt taaaatccaa atatggcttg tattgtaaac tgtacttttc
    11521 tctaattctg aataataatt agtacaaaac aaagaatgta gttaccatgc ccaagtttta
    11581 gatcatgaat ttcagcgcta ataaaataca attattcttg tgaagggtat tcaacaaggg
    11641 agtatactat aaactagtga tatggaatta actaaactat atctaaaatt tattatttac
    11701 cgacatgccg atattttacc ggtcatcggt ctatgcctga ccattctgga gatgatgtca
    11761 tccaggattt cacctttcat gcggt
//
LOCUS       DAOQQN010000043        11713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1935_length_11713_cov_5.028135, whole genome shotgun sequence.
ACCESSION   DAOQQN010000043 DAOQQN010000000
VERSION     DAOQQN010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11713
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1935_length_11713_cov_5.028135"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PLG24_02650"
     CDS             <1..645
                     /locus_tag="PLG24_02650"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HMZ23814.1"
                     /translation="MGFRLPENFNSPYTSGSITEFWRRWHITLGKWMRNYLYIPLGGN
                     RISSGRTYMNLWLVFVASGLWHGASWNFVIWGAYHGCFLVLERTFLLRLYNRIGRVVP
                     IVLTFFFVVIGWVFFRVEHFNDALTYISRLFAGGMEQFSLPNGRFIAIFLIAVFFAFF
                     AVAQRTYAFQQKVFEQNYSLRGHLLMTPVILILFVLSLASITSFGFNPFIYFRF"
     gene            651..1781
                     /locus_tag="PLG24_02655"
     CDS             651..1781
                     /locus_tag="PLG24_02655"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23815.1"
                     /translation="MRGGRKYLLLFWLIVLIAPLLQSGFKVFKGGALKGAIIPVPDVE
                     FTLKGWFDGSYQQGKEEYLNEHFGFRNDLVRLHNQINFSLFKIASVRDAVVGKENYLF
                     ERKYIEEYYGRNFIGDSAIDRRIFMIQRIADTLNSLNKKLIVVMSPSKARFFPEYIPD
                     SLRGPIVPGNYEKYAEGFSRTGIVTMDCNKYFANLRDTCSYPLFARYGIHWSLYGASF
                     VRDSLLTLIGRVTNKSLPRWDKQFYVSPTPLDTDNDLGDAMNLIVKLKPGPLAYPTYT
                     LPETVQPVPVRLISVGDSFYWTMTSLKIHEALVDDQFWYYNSEVWPPRFGANNNRLTY
                     ADLNIKDEIYNNEVFLLICSEINISNIGFSFVDDAYKLFYGQ"
     gene            complement(1790..2719)
                     /locus_tag="PLG24_02660"
     CDS             complement(1790..2719)
                     /locus_tag="PLG24_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ23816.1"
                     /translation="MNNNILRIRAVSKCFEDHQALNSVSFDVPKGGVFGLLGPNGAGK
                     TTLIRIITNITQADEGSVIFEGTDTKYLDRGLIGYMPEERGLYKKMKVAEQLEYFAAL
                     RNMPVQEARQRIGFWLERLNISDWAYKKIEELSKGMQQKIQFIATVLHNPSFLILDEP
                     FSGLDPVNTSLLKDEILRLNQLGTTIMFSTHRMEQVEEICDRIVLISKGKNILDSTVK
                     ETKETWKEGLFHLEFEHTIEFTGDMPEGIEIKENKGNELYVKAPNLRNLNTFITSQIN
                     KGNLLHSMKERLPTLNEIFIKTVRRENPVPENS"
     gene            2776..3297
                     /locus_tag="PLG24_02665"
     CDS             2776..3297
                     /locus_tag="PLG24_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23817.1"
                     /translation="MKMTILLLNKSMLLLLLISFARPGHAQIAGPTFDSLRTARERSL
                     FVKNEILHLVGMGRVTAVRSRKSCNCGCNIIKESFRQKGRSDYIDVFDFCDSYVFEYT
                     SMEESGADRFDELMQILREHGFKFVDNPILIKDRFRVKHRVVEGIDPLTFMEEKIYKS
                     GKCLIRISLSTPE"
     gene            complement(3283..4080)
                     /locus_tag="PLG24_02670"
     CDS             complement(3283..4080)
                     /locus_tag="PLG24_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23818.1"
                     /translation="MPEILHENSGMIFIGDTIAWVNDSDNPAAVILTDKKGIYLKTVP
                     LPTRNIDWEEISADDYGNLYIGDLGNNRLNRSEFKIYKLNHQLLLIDSIQFSYPENIA
                     GPKTFDCEAFCLFDGKFYLFTKGIDPNHPFQLCIFRIDDEKNNTRPEQLDCTNILKPY
                     VVTGAAISPDKKTLALLTYRYHWIAGILPDTDSRIYLFNLNDGMENIWNHPVRYYTLP
                     TWFTTRQFESIGYDSKVDIWIACERTAFLKPQLRKIKLPARLKSFRR"
     gene            4256..5353
                     /locus_tag="PLG24_02675"
     CDS             4256..5353
                     /locus_tag="PLG24_02675"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HMZ23819.1"
                     /translation="MTFEGDDKKAIRSLRVSKVIWPILIGVGVVGYLFWKQFDINEFN
                     KIHWTRGAFIAILLSIGFMICKHLSYAMRLYILSEKEFSFLKCIQLIFIWEFSSAIAP
                     TAVGGSAVALFALAQEKLSAARTTVIVIYTAILDSYFFVISLPILFMIFGPEMVRPGM
                     KVLDFSTVAGSALLSAYIFKTIYSTLFLYGLFIDPRKIRSLLSAVTSLPLLNRWHDKA
                     VKLGDDIILASGEMKGKSFRFHFGAIWTTCFAWIFRFLILVTLILGIIGGLERGWYVV
                     LELYARVQTMFMVIMFSPSPGGSGIIEGVFGGFLSDYIPVGVALIIALLWRLISYYFY
                     LFAGVIIVPNWLNKIISQRHKAEQGEQVSGD"
     gene            complement(5465..6325)
                     /locus_tag="PLG24_02680"
     CDS             complement(5465..6325)
                     /locus_tag="PLG24_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23820.1"
                     /translation="MLIAYKRVNNFKAAIMSFYRVFTLCILVVVLRSPANAQEWMVDS
                     VQHESGLLAIKIDALGNHYVVTEEMQIIKYNPKGTIRNTYNNNRLGSIGRLDVSNPFL
                     ILVYYPQYQTAVLLDNNLSESGRIRFSDIGIGNIRSAAVSDDNNLWVFDEGARNIIKV
                     STNGHILFKGIPCFNFDIDDSRPFEIMQRGGYLYICQPDLPVRIFDIYGKWVKSLLLD
                     NFQAMIYLDKIIYYKADNKIYSHNTELPMLGDTVLWEAGNVYSICYDAREICFYGINS
                     TGEIVKLNVR"
     gene            complement(6975..7340)
                     /locus_tag="PLG24_02685"
     CDS             complement(6975..7340)
                     /locus_tag="PLG24_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ23821.1"
                     /translation="MRKIFTLILFAFAFYLPAVASVCGDMVQHKSHVVYLFQQQPGDE
                     IDIKVSPNPANDYFSVTSNVTYNRIILYNLLGKPLKSYRPTVDNTYTLTDLPGGIYIL
                     RFLGDDNHVIKTVKLYKKD"
     gene            complement(7632..9563)
                     /gene="thrS"
                     /locus_tag="PLG24_02690"
     CDS             complement(7632..9563)
                     /gene="thrS"
                     /locus_tag="PLG24_02690"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HMZ23822.1"
                     /translation="MINITLPDGTIKQYPKGTTSMQIAESISMGLAQNVLSAKVDGEV
                     WDASRPINADASLQLLTWNDKDGRATFWHSSAHLMAEALESLYPGVKFGIGPAIETGF
                     YYDIDTGDKTIGLADLEAIEKKMAELAGHKNHYIRKEVSKADAIQYFTEKGDQYKLEL
                     IDGLEDGQITFYTQGGFTDLCKGPHIPDTGKIKAIKLTNVAGAYWRGDEKRPMMTRIY
                     GVTFPKQKELQEYLTMIEEAKKRDHRKLGAELELFMFSDLVGAGLPIWLPKGAALRGR
                     LEEFLKSEQIKRGYQQVITPHIGKKELYETSGHWAKYGADSFQPIHTPKEDEIFLLKP
                     MNCPHHCEIFGHKPRSYKELPLRIAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCT
                     PDQLKDEFLNVLDLTMYVIRKLGFDSFTAQISLRDPENKEKYIGSDEVWEKAESAIID
                     ATREAGLETVTELGEAAFYGPKLDFMIKDALGRRWQLGTIQVDYNLPERFNLEYIGAD
                     NTTHRPVMIHRAPFGSMERFIGVLTEHTAGKFPLWLAPEQYAILPISERFVDYCKEIQ
                     ARLSAVDIRGILDERNETIGRKIRDTELKRIPYMLIVGEKEVEENKVAVRKQGAGDQG
                     AMNVEDFITYFKKELEGNH"
     gene            complement(9730..10365)
                     /locus_tag="PLG24_02695"
     CDS             complement(9730..10365)
                     /locus_tag="PLG24_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ23823.1"
                     /translation="MRIILTSIILILLTSSILAQNLGFKFGIKAGFSQTDYGIKELHI
                     QSTDDFKMAIEDSRVGYHGGITLQLRIRKFILQPEVIFNTINVNYKLTGIFSGTIDTI
                     LAKETLQDLDIPIMLGFKSGILRLNAGPVGHLYLDSSSDLFKVSGYSEQFDKIKWGWQ
                     AGLGLDIWKFTIDLRYEGNFSKFGDHIRFGGKSYSFSDRPQRLIASLGLAF"
     gene            complement(10894..>11713)
                     /locus_tag="PLG24_02700"
     CDS             complement(10894..>11713)
                     /locus_tag="PLG24_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HMZ23824.1"
                     /translation="GRLLVGAAVGVTGDTEERISALVQMDVDVIIIDTAHGHSQGVLD
                     TIRRIRRTFPEIQLIAGNVATGEGAKALADAGVNGIKVGIGPGSICTTRVVTGVGVPQ
                     LTAIHNASKAVAGSNIPVIGDGGIRYTGDIVKAIAAGADSVMLGSLFAGVEESPGETI
                     ILDGRKFKSYRGMGSLGAMQSGSKDRYFQDVEDDVKKLVPEGIEGMVPYKGTLAEVIY
                     QYIGGLRAGMGYCGAANIEALKRARFVRISAAGVNESHPHNINITKASPNYSKR"
BASE COUNT         2964 a         2404 c         2625 g         3720 t
ORIGIN      
        1 atgggtttca gactgccgga aaatttcaac agtccttata cttccggtag cattaccgaa
       61 ttctggcgac gttggcacat tacactcggc aaatggatgc gcaattacct ttatattccc
      121 ttgggcggaa accgcatttc ttcgggtcgt acctatatga atctctggct tgtctttgta
      181 gcttccggtc tctggcatgg ggcatcctgg aattttgtta tatggggcgc ataccacggc
      241 tgctttctgg tacttgagcg aacctttctc ctccgccttt ataatcgaat cggccgggtt
      301 gttccgattg ttctgacctt cttttttgtg gtgatcggct gggtgttttt cagggtggaa
      361 catttcaatg atgctctgac ttacatcagc aggttgtttg cagggggcat ggagcaattt
      421 tcgctgccca atggtagatt tatcgccata ttcctgattg ctgtcttttt tgctttcttt
      481 gctgtcgcac agcgtaccta tgcttttcag caaaaagtat ttgagcagaa ctattctctg
      541 agggggcatc tcctgatgac tcctgtgatt ttgatattat tcgtattgtc cctggcttca
      601 attacgagtt ttggatttaa tccattcatt tatttcagat tctgataggc atgagaggag
      661 gaagaaaata tttgttatta ttttggttga tcgttctgat tgctccgttg ttacagtccg
      721 gttttaaggt attcaaaggt ggagcgttga aaggtgctat cataccggtt cctgatgtcg
      781 agtttacctt aaaaggttgg tttgacggca gttaccaaca gggcaaggag gaatatctca
      841 atgaacattt tgggttcaga aatgatctgg taaggttaca caatcagata aacttctcgc
      901 ttttcaaaat tgcaagtgtc cgggatgcag tggtaggtaa ggaaaattat ctttttgagc
      961 ggaaatatat cgaagaatac tatggccgca attttattgg tgattccgca atagataggc
     1021 ggatttttat gattcaacgg attgcggata cacttaactc gttgaataag aagttaattg
     1081 tcgtaatgtc gcccagcaag gcgagattct ttccggagta tatacccgat agcttacgtg
     1141 gtcccatcgt tccgggcaat tatgaaaaat atgccgaagg tttttcccgg acaggtatcg
     1201 ttactatgga ctgcaacaag tattttgcca atttacggga tacttgttcc tatccgctgt
     1261 ttgccaggta tggcattcat tggtcattgt atggagcgag ttttgtaaga gattcgttat
     1321 tgacgctgat tggcagggta actaataagt ctttgccccg ttgggacaag caattttatg
     1381 taagccctac accccttgat acagataatg atttgggtga tgcgatgaat ctgattgtca
     1441 aattaaaacc aggaccgttg gcttatccca catatacatt gccggaaaca gtacagcctg
     1501 tcccggtcag gttaatttcc gtcggggata gcttttattg gacgatgacc agcttgaaga
     1561 ttcatgaagc attggtggat gatcagttct ggtattataa tagtgaggtg tggccaccgc
     1621 ggtttggggc aaataataac agactgactt atgcagacct aaatattaaa gatgaaattt
     1681 acaacaatga agtctttttg ttgatttgtt cagaaatcaa tatcagcaat attggtttct
     1741 cattcgtaga tgatgcttac aagttattct acggccaata aaggagtgat taagaatttt
     1801 caggaacagg gttttctctt ctcaccgttt tgatgaagat ctcgttgagc gtaggcaagc
     1861 gttccttcat gctgtgtagt agatttccct tattgatctg gcttgtaatg aatgtattca
     1921 gattgcgaag gttgggagcc tttacataga gctcattgcc tttgttttcc ttaatttcaa
     1981 ttccttcagg catatcaccc gtaaattcga tcgtgtgctc aaattcgagg tggaagagac
     2041 cctccttcca cgtttccttg gtttccttta cggtgctgtc caatatgttt ttgcccttac
     2101 tgatcaatac gatgcgatca catatttcct cgacctgctc cattcggtgg gtcgagaaca
     2161 tgattgtcgt gcctagttgg ttcagcctca ggatttcatc tttcaaaagg ctcgtattga
     2221 ccgggtcaag gcctgaaaat ggctcgtcga gtataagaaa ggagggattg tgcaggacgg
     2281 tagcgataaa ctgtatcttt tgctgcattc cctttgacag ttcttcaatc ttcttataag
     2341 cccaatcaga aatattcagc ctttcaagcc agaacccgat cctctgccgg gcttcctgaa
     2401 caggcatatt tctcaatgca gcaaaatact ccagctgctc tgcaaccttc atttttttat
     2461 acaatcctct ctcttcgggc atataaccga tcaggccacg gtcgagatat tttgtatctg
     2521 tcccctcgaa tataacagag ccctcatccg cctgggttat gttggttata attcttatca
     2581 gtgtagtttt tccggcgccg tttgggccga gaagtccaaa aacaccacct ttggggacat
     2641 caaagctgac actgttgagt gcctgatggt cttcaaaaca tttcgaaacc gcccggatgc
     2701 ggaggatatt attattcatt acaaattctt tgaggcgaag ataaataaat tgagactttg
     2761 tcttagcttt attcgatgaa gatgaccatt ttgctgttga acaaatccat gttgttgttg
     2821 ctattgatat catttgcccg gccaggacat gcgcagatag caggaccgac gtttgattcg
     2881 ctgcgtacag ccagggagag gtctttattt gtaaaaaatg aaattctcca tctggtgggc
     2941 atgggcaggg tgacggcagt tagatctcgt aaaagctgca actgtggttg taatatcatc
     3001 aaggaaagct tcaggcaaaa ggggaggagt gattatatcg atgtttttga cttttgtgat
     3061 agctatgtct tcgaatatac ttcgatggaa gaaagtgggg cggatcgatt cgatgagttg
     3121 atgcaaattt tgagggagca tggtttcaag tttgtggata atccgatcct gataaaggat
     3181 aggtttcggg taaaacacag ggttgtcgaa ggaattgacc cactcacctt tatggaagag
     3241 aaaatctata aatcgggaaa atgtctgata agaatcagcc tttcaacgcc tgaatgattt
     3301 cagacgggcc gggagtttta tttttcttag ctgtggcttt agaaaggcgg ttctttcgca
     3361 ggctatccag atatccactt tgctgtcata accgatcgat tcaaattgtc ttgtggtgaa
     3421 ccaagtcggt aaggtatagt atcggaccgg atgattccag atattttcca taccatcatt
     3481 gaggttgaat aaatatatcc tggagtctgt atccggcagg attcccgcta tccaatgata
     3541 acggtaagtg agcaatgcca gggtcttttt gtccggactt atggcagcgc cggtgacaac
     3601 atatggcttc aggatattgg tacagtccag ctgttcgggc cgggtattgt ttttttcatc
     3661 atcaattcgg aaaatacaca gttgaaaagg atggtttgga tcgatgcctt tggtaaataa
     3721 ataaaacttt ccgtcaaata gacagaatgc ttcacaatca aaggtttttg gccctgcaat
     3781 attttccgga tagctgaatt ggatggaatc aatcaaaagt aattgatgat tcaatttgta
     3841 aattttgaac tcggaacgat tcagcctatt attgccgagg tctccaatat ataaattgcc
     3901 gtaatcatca gcggaaatct cttcccaatc tatgtttcgt gtgggtaatg gcacggtttt
     3961 caggtagata ccttttttgt cggtcaggat cacggccgcc ggattatcgc tgtcattcac
     4021 ccatgcaatg gtatcgccga taaagatcat gccggaattt tcatgtaaaa tttcgggcaa
     4081 aagcctttta ttttggcagt ttgttgaatg gattaacagg ttaaaaacaa tgattattaa
     4141 aaaaaatgaa aaattattca aatctttatg cgttttatcg gtaattgata tcgaaatttt
     4201 gattcgtgat taattatcct tgccaaattt ataaaaagaa aagcgtacgt ccataatgac
     4261 ttttgaaggt gatgataaaa aagccatacg gtctctaagg gtctctaagg tcatttggcc
     4321 aatactgatt ggagtgggag tagttggcta ccttttctgg aagcaatttg atatcaatga
     4381 gtttaacaag attcattgga cccggggagc ctttattgct attttgcttt ctattggatt
     4441 catgatttgc aagcatttga gttatgcaat gaggctatat attctttctg agaaagaatt
     4501 cagctttttg aagtgcattc agcttatctt catctgggaa tttagttcag caattgctcc
     4561 tactgctgtt ggcggttcag cagttgctct atttgcactc gcacaggaaa aactttcagc
     4621 cgcacggact acggttatcg tgatatatac ggcgattctg gattcctatt tctttgtgat
     4681 atccttaccg attctcttta tgattttcgg tcctgaaatg gtccgtccag ggatgaaggt
     4741 attggatttt tctacggtag ccggtagtgc tttgttaagt gcttatattt tcaagaccat
     4801 ctatagtacg ctgtttttat atggactatt tattgacccg aggaaaataa ggtcattact
     4861 gtcggcggtc acatctcttc ccttactgaa tcggtggcac gataaggcgg tcaagctcgg
     4921 ggatgacatt atacttgctt ccggtgaaat gaaggggaaa tcgtttcggt ttcactttgg
     4981 cgcgatatgg acaacttgtt ttgcctggat ttttcggttt ttgatccttg ttacattaat
     5041 cctgggcatc atcggtggat tggaacgggg atggtatgtt gttttagaac tttatgcgag
     5101 agttcagact atgtttatgg ttattatgtt cagtccttca cccggcggat ccggtattat
     5161 tgaaggggtg ttcggtggct ttttgtcgga ttatattccg gtgggcgtag cgctgatcat
     5221 cgcgttattg tggcggttaa tctcctatta cttttatctt tttgccgggg tgattatcgt
     5281 gcccaactgg ctgaataaaa ttatttccca aaggcataaa gccgagcagg gtgaacaagt
     5341 ctcaggcgat tgagtgtttg tctaatttta agttgtttaa aagatactgc taaaagggtg
     5401 tgcctatagt tgcagattga ttttttacct aaggccggtt cttgtaagtt attgttttcc
     5461 agttttatcg aacatttaat ttcacgatct cgcctgtcga attgatgccg tagaagcaga
     5521 tttcccgggc gtcatagcag atagagtaaa cattgcctgc ctcccacagg acggtatcgc
     5581 cgagcatagg aagttctgta ttatggctat agattttgtt gtctgctttg taataaatga
     5641 tcttgtcgag atatatcatc gcctgaaaat tgtccaacaa cagacttttt acccattttc
     5701 cataaatatc aaagattcga acaggtaagt ccggctgaca tatatataaa tagccccctc
     5761 tttgcatgat ttcaaatggc ctggagtcgt caatatcaaa gttgaagcaa gggatgcctt
     5821 taaaaagaat atgtccattg gtactgacct tgataatgtt tctggctcct tcgtcgaata
     5881 cccaaaggtt gttgtcatcg cttaccgcag cggagcgtat gtttcctatg ccgatgtcag
     5941 aaaatctgat tctgccactt tccgacaaat tgttatcgag aaggacggct gtctgatatt
     6001 ggggatagta aaccaaaatc aggaaaggat tactgacatc caacctgcct atgctgccaa
     6061 gccgattgtt gttataggta ttccggatgg taccttttgg gttgtatttg attatctgca
     6121 tttcctctgt tacaacataa tgattgccca atgcgtcaat tttaattgcc agcagaccgg
     6181 attcatgttg cacggaatcc accatccatt cctgtgcatt tgccggagat ctgagcacga
     6241 caacaagtat gcaaagagtg aatacccgat agaacgacat tatcgccgcc ttaaaattat
     6301 ttactctttt ataagcaatc agcatcttat gagtctactc tttaattaaa actgcttttt
     6361 catctatcct attttccagc tcaatcattc aataggtcac tcttagcagt tttttttgtc
     6421 gttttgattt attcatttct caacacgatg ttatattaag gatttttcgt gaaaaaagtt
     6481 ccgatgggct gctgccattt ttactaaaca gttgattttt cgcttgcttt gaaattggca
     6541 agatacttga tcatttttcc ttcaatgtat ccttccggta gttcgaagat ttgcagctga
     6601 gtcacatttt tatagaaaaa tggaggatga tggagatgct ttattggcag aaagagttag
     6661 gttattttgc ggtgctgagt taaaaaatga agtaggtaca taattaataa cggagcacca
     6721 gggtataggt tgaattgtaa tagttggcaa gaataatgtt tatcaatttt gagataagtt
     6781 ttttatcgga ttgctcttat gtgacgattt gcattacccg ggcgattatt aatcatctac
     6841 ctacttggaa atttgagcat ctcatgattt ttcggcaaat ctgttgattt ctcatcttgt
     6901 caaaggacga ggttacaatt cgcactgaat gcggggaaaa acttagtaag aaaatgaatg
     6961 gagtaagtcg ggggtcaatc cttcttgtat aacttaaccg tcttaattac gtgattgtca
     7021 tcccccagaa atcgtaggat gtaaatcccg cccggaaggt cagtcaaggt atacgtgtta
     7081 tcaacagtag gtctgtaact cttaagtggc ttgccgagca gattatacag gatgatcctg
     7141 ttgtaagtca cattggatgt aacggaaaaa taatcattgg caggattcgg agagacctta
     7201 atatcaattt catctccggg ttgttgctgg aataaataaa ctacatgact tttatgttgc
     7261 accatgtctc cgcagactga agcgacagcc ggaaggtaga atgcaaatgc aaaaagtatc
     7321 agtgtaaata ttttcctcat atgttatttg aaccccaaat ataagtgaaa ggttttttta
     7381 atgatttaaa agtagtgaaa ttttatctca aaatattgtt aaattttcaa ttaaacattt
     7441 atttcatgaa gttttgttct gacacccaga ggttggaaat ctttaaaatg ctttgtatca
     7501 ggaggatagt atgacagtgt catgaggtgg tcatgcacag gtctgactat tctcttcctg
     7561 aatgatttta tcctgattgc cttctttatt cccttatgca attcagtgtc tcacaccttt
     7621 gaggatgggt atcagtgatt tccttccagc tcctttttga aataggtgat aaaatcctca
     7681 acattcatag cgccctggtc gcctgctccc tgcttcctta cagctacctt attttcctca
     7741 acttcttttt cgcctacgat taacatgtag ggaattctct tgagttcagt gtcgcgaatc
     7801 ttacgcccta tggtttcgtt tctttcgtcg aggataccac ggatgtcaac cgccgacaat
     7861 cgggcctgaa tttccttgca ataatcgaca aagcgctcgc tgattggaag tatcgcatat
     7921 tgttcaggtg cgagccagag tgggaacttg ccggcagtat gctctgtcag tactccaatg
     7981 aagcgctcca tggatccgaa tggtgcgcga tgtatcatga caggtctgtg tgttgtgtta
     8041 tcggctccga tatattccag gttgaaacgt tccggcagat tgtaatccac ctggatggta
     8101 cccaactgcc atcttcttcc cagggcgtcc ttgatcatga agtcgagctt gggtccatag
     8161 aatgccgcct ctccaagttc ggtgactgtc tccaatcctg cttctctggt tgcatcaatg
     8221 atggcacttt cagccttctc ccatacttcg tcactgccta tgtatttttc tttgttttca
     8281 ggatcgcgta aggatatctg cgctgtaaaa ctgtcaaagc ccaacttacg gattacgtac
     8341 atggttaagt cgagcacatt gaggaattcg tccttgagct ggtccggagt acagaatata
     8401 tgggcatcat cctgagtaaa ccctcttact ctggtcaatc cgtgcaactc accactttgt
     8461 tcataacgat atacggtacc aaattctgct atcctcaaag gcaattcctt ataggagcgt
     8521 ggcttatggc cgaatatctc gcagtggtgc ggacagttca taggtttcag aaggaagatt
     8581 tcgtcttctt taggtgtatg gatgggctgg aatgaatctg cgccatactt cgcccagtga
     8641 ccagaggtct cataaagctc cttttttccg atatggggag tgatgacctg ctgataacca
     8701 cgtttgatct gctctgactt gaggaattcc tccagtctgc ccctcaatgc ggcaccctta
     8761 ggtaaccaga ttggcaagcc ggcgccgacg aggtcagaga acataaagag ttcgagttct
     8821 gcgccaagtt ttctgtggtc acgcttttta gcctcttcga tcatggtcag gtattcctga
     8881 agttccttct gtttggggaa ggtgacgcca tagattcggg tcatcatagg acgtttttcg
     8941 tcgcctcgcc agtaagcacc ggcgacattg gtcagtttga tggctttaat cttccctgta
     9001 tccgggatat gcggtccttt gcacaggtca gtaaatccac cctgtgtata gaaggttatc
     9061 tggccatctt ccagcccgtc aatgagttcc agcttgtatt ggtcaccttt ttcggtgaag
     9121 tattggatag catcagcctt ggaaacctct ttgcggatgt aatggttttt atgccctgcc
     9181 agttccgcca ttttcttttc tattgcttcc agatctgcta agcctatggt cttatctccg
     9241 gtatctatgt catagtaaaa tccggtctcg atggcaggtc cgataccgaa cttgactccg
     9301 ggatacaaag attccaatgc ctcggccatg agatgtgcgg aagagtgcca gaaagtcgcc
     9361 cttccatcct tatcattcca ggtcaatagc tgcagacttg catcagcatt gatgggacgt
     9421 gatgcatccc agacctcgcc atcgactttg gcagacagga cgttttgtgc aagacccatg
     9481 ctgatggatt ctgcaatctg catcgaagtt gttccttttg ggtattgctt tatggtgccg
     9541 tccggcagag ttatattgat cattttttct aagtcttaaa attgggtgca aaattgagaa
     9601 aacaatacga gaataataaa taaatgtaaa gaaatgttgc agggcaggtt tatggtatgt
     9661 cgttcttcca aacatccatt ctctgaaaga aaatgcggga atgcaatgat agattgaatg
     9721 atttttgcct cagaaggcca gtcccaatga agcaatcagc cgttgtggcc tgtccgaaaa
     9781 ggaatacgat ttgccaccga agcggatatg gtcaccgaat ttggagaaat tgccttcata
     9841 tctgagatca attgtgaact tccagatatc cagccccagt ccggcctgcc aaccccattt
     9901 gatcttgtca aattgctcac tatagccgga taccttaaac aaatcactgg aactatccag
     9961 gtacaaatgc cctacaggcc ctgcattcag tctaagtatc ccggacttga atcccaacat
    10021 aatggggata tccaaatctt gcaatgtttc tttagccagg atcgtatcta tagtgcccga
    10081 aaatatgccg gtgagtttat agttgacatt gatggtattg aatattactt ccggttgaag
    10141 gatgaatttt ctgattcgca actgtagcgt gatgcctccg tgatatccca cccgactgtc
    10201 ttctatagcc attttgaagt catccgtact ctggatatgc aattccttga taccataatc
    10261 cgtctgacta aaaccggcct tgatgccaaa tttgaatccc aaattttgag ctaatattga
    10321 actagttagc agaatcaata taatggaagt gaggataatc cgcatgttgt atatttaaag
    10381 tgttgagtta tacattatta tatgttatgc aaatatataa ttactattta tttctcttat
    10441 aacatttatt ctttttaacg aaaaatggat gaagaaattt cttgtcgaat tggaaatgga
    10501 ggatgctttt ttggattcta ccattctcat ttcgatactt cgaaagtgca ccaatattct
    10561 gaagggtggt taatttggtg taaatttagt caatttgagg tattagccgt tgtattttcc
    10621 agtttagcca ttttcttcgg cgtctttcat taaacttcgg ttcatgcttc tattggactg
    10681 atgtcaagat gttcagcccg atgcgtgagg gatagaaacg gcacgagcga tgtccatttc
    10741 tcatgatagc ctgcgattga cctctatccc aatatgcctg ttgaccggcc cggagcggat
    10801 aagcgagtac agtgaatagc ccgacccgtt aggggcacgc cataataatg acgaccggat
    10861 ttattccctg acaatgggat ttgttttttt cgtttatcgc ttggaataat taggcgatgc
    10921 cttagttatg ttgatgttgt gaggatggct ttcattgact cctgctgcgg aaatacggac
    10981 gaagcgggct cgtttcagag cttctatgtt ggccgctcca cagtagccca tacctgctct
    11041 caggccgcct atgtactgat atatcacttc tgccagcgtg cccttgtatg gaaccattcc
    11101 ttcgatgcct tccggaacca gtttcttcac atcgtcctct acatcctgga aatacctgtc
    11161 tttggaacct gactgcatcg caccaagcga acccatacca cggtacgact taaatttcct
    11221 gccatcgagg atgatggtct ctccgggtga ctcctccacg cctgcgaaga gggagcccag
    11281 catgactgaa tccgcccctg cagctatcgc cttgactata tcaccggtat atctgatgcc
    11341 gccatctccg ataacgggga tgttgctgcc ggcgactgct ttggatgcat tgtggatggc
    11401 tgtcagttgg ggtacaccaa ctccggtaac gacccgtgtg gtgcaaatac ttccggggcc
    11461 tatgccgacc ttgatgccat tgaccccggc atctgccagc gctttggcgc cttctccggt
    11521 agcaacatta cctgctatta actggatctc cggaaaggtg cgtctgatcc gccggattgt
    11581 gtccaacact ccttgtgaat ggccgtgggc tgtgtcgatg atgattacat caacgtccat
    11641 ctggacaagg gcactaatcc tttcttccgt gtcaccggtt actccgacgg cagcacctac
    11701 cagaagccgg cca
//
LOCUS       DAOQQN010000044        11627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1961_length_11627_cov_5.677497, whole genome shotgun sequence.
ACCESSION   DAOQQN010000044 DAOQQN010000000
VERSION     DAOQQN010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11627
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1961_length_11627_cov_5.677497"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /gene="lnt"
                     /locus_tag="PLG24_02705"
     CDS             <1..788
                     /gene="lnt"
                     /locus_tag="PLG24_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HMZ23825.1"
                     /translation="ETVLDPVNMDDYYGQNPGLAKLHTLFRLAPKSKILLGVGGYHIF
                     NEDPGRSTIRRSDGLLYEMYNAAIMLESDSVVEEYHKSLFVPAAEIFPFKKILPFLKP
                     LVHKLGGTYEGFAAQPFPTNFKFRDSLNVAPIICYESVYGGWVRQYVERGAGLLAIIT
                     NDGWWDNTPGHLQHLAIGRLRAIENRKDIARAANTGTSCFINQRGDVRMATSYETDAV
                     INDLVYYSEEKTFYTRHGDYVIFLTAIITAFLLIFNFIPNKRG"
     gene            1164..2024
                     /locus_tag="PLG24_02710"
     CDS             1164..2024
                     /locus_tag="PLG24_02710"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HMZ23826.1"
                     /translation="MKFYTFILTFVFLPFVQAQSSKFDFLVGTWKIDGKESFEHWDQL
                     SANTLKGLTYNKTKGAITVTEYLDLRYEGNHWVYSATVKGQNQGEPTDFRLIQDDTVF
                     VFENMKHDFPQQIIYQKRESDEINITLSASGHKSVSYLMRREGRLPQPIGSGNSNPHY
                     DASLAQRLEGDDYGMKQFVLVMLKTGSNKSKDKDLINKSFRGHLDNINLLVSQGKMTV
                     AGPLEDKSGIYRGIFILNVTTLEEAKALLEGDTAISAGFLDYDLYPWYGSAALPEYLD
                     AADKIWRKKP"
     gene            2084..2440
                     /locus_tag="PLG24_02715"
     CDS             2084..2440
                     /locus_tag="PLG24_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ23827.1"
                     /translation="MLCTIHPKLPMRNKAVTAEYYIGNLGFQPIGDSDFEGYLMLKKD
                     QIELHFFEYKTLVPAENYGQVYIRTDDIASLYRELTDKNVPIHPNGPLEIKPWGQIEF
                     SLLDPDSNLLTFGQGH"
     gene            2841..3125
                     /locus_tag="PLG24_02720"
     CDS             2841..3125
                     /locus_tag="PLG24_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HMZ23828.1"
                     /translation="MQKVIIEEGVVHVVPEDIKAVLVANEALLDKWNSLTPLMRNEWI
                     CWTTIVKQESTRKEHIRRLEEDILDGKKRPCCWPGCPHRRESARKWFNKM"
     gene            3383..3604
                     /locus_tag="PLG24_02725"
     CDS             3383..3604
                     /locus_tag="PLG24_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23829.1"
                     /translation="MKTIDTFIVHPQTNEQVAALKAFMQALKIKFEVTKEEEAYNPEF
                     VAKIEKAKQDYKEGKGKVYTSEQLNELWK"
     gene            3696..3854
                     /locus_tag="PLG24_02730"
     CDS             3696..3854
                     /locus_tag="PLG24_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HMZ23830.1"
                     /translation="MKLHPYSGLGKPEPLKYNLSGVWSRRINREHRLVYEVTDNIICI
                     LSVWGHYK"
     gene            complement(3965..4243)
                     /locus_tag="PLG24_02735"
     CDS             complement(3965..4243)
                     /locus_tag="PLG24_02735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HMZ23831.1"
                     /translation="MSFTLEFTKTALEDIEKHKLAGDKKVLSKLEQLLNELREHPLTG
                     TGRPEALRHDLQGLYSRRINRKHRLVYGIKEESVVVIVVSAYTHYGDK"
     gene            complement(4240..4491)
                     /locus_tag="PLG24_02740"
     CDS             complement(4240..4491)
                     /locus_tag="PLG24_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23832.1"
                     /translation="MLVISSREFRQNQSMYLDKVDKGEQVIVQRGKDKSYMLTAIKED
                     DIYFNAEMVNRIKKSLNEASNGEVKRVSTPEEIRDLLGL"
     gene            5326..5556
                     /locus_tag="PLG24_02745"
     CDS             5326..5556
                     /locus_tag="PLG24_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23833.1"
                     /translation="MKNQDMMIVYPDYEEPINVLKVFFNAQKIKFEPSNESHTPLSLA
                     KKLKRHDKTIKKEETRFILPSNSIQYGSNLSA"
     gene            5939..6151
                     /locus_tag="PLG24_02750"
     CDS             5939..6151
                     /locus_tag="PLG24_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23834.1"
                     /translation="MHTIDLIREIQRLPISKRLYVVEETIKSIKKEEMQHQMELAAND
                     LYNDYVNDKELTAFTSLDLENFYEAK"
     gene            6138..6476
                     /locus_tag="PLG24_02755"
     CDS             6138..6476
                     /locus_tag="PLG24_02755"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HMZ23835.1"
                     /translation="MKQSEVWLIDLDPTKGAEIQKKRPAIIVNNDSLGKLPLKVIVPI
                     TDWNDRYEIAPWMVKIEPNSTNGLSKTSSVDCFQIRSLSQERLIKKIGFIDGATLYEI
                     KEAIRKVLDL"
     gene            complement(6636..6708)
                     /locus_tag="PLG24_02760"
     tRNA            complement(6636..6708)
                     /locus_tag="PLG24_02760"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6673..6675),aa:Arg,seq:cct)
     gene            complement(7083..7331)
                     /locus_tag="PLG24_02765"
     CDS             complement(7083..7331)
                     /locus_tag="PLG24_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23836.1"
                     /translation="MVTSIPAVVDEVAEVPMLGVYSLKGIISGNWTDAKYQALGVFLP
                     GVSKTTIKTLSEGFEHAAKASTKLPTQIHHITTSKHSV"
     gene            complement(7347..7574)
                     /locus_tag="PLG24_02770"
     CDS             complement(7347..7574)
                     /locus_tag="PLG24_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23837.1"
                     /translation="MSVYNYAENEPMGSIDLWGHQRVYVMQPDKNGIVTPTFTIGSTT
                     YQGRNVTRPPQYHGSQPSIGPAQQTSLDPVA"
     gene            8063..8428
                     /locus_tag="PLG24_02775"
     CDS             8063..8428
                     /locus_tag="PLG24_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ23838.1"
                     /translation="MDIAKNIKKLREERGLMQKEVANAVGVHPSNYSKMEKGEREFGI
                     EVIDKLAKFFGLTIDELVHPNNKLPKVVKVEDKTAIEKVQLISQLDEEDKNAVYRIID
                     GMLTKNKFQTFFEQNIAVK"
     gene            8436..8645
                     /locus_tag="PLG24_02780"
     CDS             8436..8645
                     /locus_tag="PLG24_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23839.1"
                     /translation="MLTVHPQYITDNAGKKISVVLSMKDFEAIMEELEELEDIRLYDE
                     AKKSNEPSIPIDDAFKMIEAKRKAN"
     gene            8645..8899
                     /locus_tag="PLG24_02785"
     CDS             8645..8899
                     /locus_tag="PLG24_02785"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMZ23840.1"
                     /translation="MAYNVTLKKRAIKALEKINEPYYSNIKEAIYSLADNPRPEGYKK
                     LKGRDGYRIRMADYRIIYDIFDNTLLVDVIDLGHRKDIYE"
     gene            complement(8958..9557)
                     /locus_tag="PLG24_02790"
     CDS             complement(8958..9557)
                     /locus_tag="PLG24_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23841.1"
                     /translation="MSKYFSLNWKHKPELASAFSLQETKLAHELIPQLEGKALLPFEF
                     ELRKVKEGKDRLIVNSDLAGLGETWLDYQSNSLAWPLMSERLKSVIEQHLTGNEGIDW
                     IRAVVNANAEQRTYYIPRFEKKLDVLDREHTKYVPGTDQILKPYFSLSKINKYAVFHD
                     ELTFNLWKITPFLNVSEALKKAIQKEKLTGLDFEKTSVA"
     gene            9939..>11627
                     /locus_tag="PLG24_02795"
     CDS             9939..>11627
                     /locus_tag="PLG24_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23842.1"
                     /translation="MKYVYLIWIFIVSIQGLTAQDLEGTYEGFKSRWKDKPVTASGSL
                     GFNTNYYAARHSGNIAIPFSYNLYGNVNLDILGINAPMGIFYANKNTTFNLPSYRFIG
                     ISPEFRGHKLHLFDRTMEFSPYSFSGTAFTGVGYEGTFGKFYVKAMAGRLNKATIHEA
                     NVLNNLDNSFKRIGWAIGGGYKDDKQQYGLHLFQAKDIASSLPDTLQTRVRPQAGSIL
                     VLDGSRRVGIFKLSMEYAYNIHTADLNAELPASGRNLFSIIPGWHPTNSSTGRHDAFK
                     SSLQIKAGKATIGLEYERIDPGYISLGTLYFNNDIETMALACQSPFFKNRLTTQVRIG
                     RQRNNLRNDQANQNVRLALSGNINARLTRTLNLGFNFSNFSFNQKSYITVRPVIDLDT
                     LIFTQNNLNTGLSLTKQFSGTNPGSIQMIINYNDARTARGDSVSNVIRLKMITASGGY
                     NKRFTDSEMNMYAGFNYNKVVALLGNSDMWGPTITVSRSFFRKFWESSLSTSFLFDQR
                     EGNNRMIFRNFWSNKLKFSENADLDVRLMYSHINNRKVAGTSEFMFQTAFLFRLN"
BASE COUNT         3624 a         2375 c         2219 g         3409 t
ORIGIN      
        1 cggaaactgt acttgacccg gtaaatatgg atgactacta tggtcagaat cccggcctgg
       61 caaagcttca cacactcttt agactagcac ctaaatcaaa gattcttctt ggtgtcggtg
      121 gatatcatat attcaacgaa gacccgggaa gaagcaccat caggcgaagc gacggactac
      181 tctacgagat gtataatgcc gccataatgc ttgaatcaga cagtgttgta gaggaatatc
      241 ataaatcact ttttgtccct gcagccgaaa tattcccatt caaaaagata ctgcctttcc
      301 tgaaaccact cgtccacaag ttgggcggaa cctatgaggg gttcgccgca cagcccttcc
      361 cgaccaattt caagtttcgg gatagtttga atgttgctcc catcatttgt tacgagagtg
      421 tatacggagg ctgggtaaga caatatgtgg aacgaggtgc cggattgctt gccatcatta
      481 ccaatgacgg gtggtgggat aacacacccg gccatcttca acatcttgcc ataggccgac
      541 tgcgggcaat tgaaaacaga aaggatattg cccgggccgc caatacaggc acttcctgtt
      601 ttatcaatca acgtggcgat gtccgaatgg ccacctcata cgaaacagac gccgtcatca
      661 acgacctagt ctactattct gaggaaaaga ccttctatac ccgacatggt gactatgtca
      721 tcttcctgac agccatcatc acagccttcc tgcttatctt taattttatt ccgaataagc
      781 gtggctgaca gggatgaccg ggcgccccac aaggatagct gagtgtcggg aaatgaaggg
      841 cgcatggtga tgatgcgtta actcataggt gcgtatcaca tcgctcactc tttctttatt
      901 aaaaaaagaa atttagaact gctaacaaaa cgttagtatg gtacaaacct ggttcgtccg
      961 gcatctatta caatatttca tatcttccgg taaaacccga accattttta aaattatcca
     1021 atcaaaataa tggaataccc ggatgaaaat tgtagatttg attacggtct tttatgggca
     1081 ttcgaacaac tctgatttaa ggataaaatt gaaagattga tatttataac tttcaacatt
     1141 gtttatcgaa aaaattttca ggtatgaaat tttacacatt tatactcacc ttcgtatttc
     1201 tgccatttgt ccaggctcaa agttccaaat ttgatttcct ggtaggcact tggaagattg
     1261 atggcaaaga atcctttgaa cactgggatc agcttagtgc gaataccttg aagggattga
     1321 cctacaataa aaccaaagga gccatcacag taacggaata tctcgacctc aggtatgaag
     1381 gtaaccattg ggtttattca gcgaccgtaa aagggcaaaa tcagggagaa cctacagact
     1441 tccgactgat tcaggacgac accgtcttcg tatttgaaaa tatgaagcat gattttccgc
     1501 aacaaattat ttatcaaaaa agagaatctg acgaaatcaa catcacctta tccgcttcgg
     1561 gacacaaatc ggtaagctac ctcatgcgca gggaaggacg attgcctcaa cccattggat
     1621 ccggaaattc caacccccac tatgacgcat ctctcgccca aaggctggag ggtgacgatt
     1681 atggtatgaa gcagtttgta ctggtaatgc ttaagacagg aagcaataaa tccaaggata
     1741 aggacctgat taataaaagt ttcagagggc atctggacaa catcaattta ttggtcagtc
     1801 agggcaaaat gactgttgcc gggcctttgg aggacaaaag cgggatatat cgcggtattt
     1861 ttatcttaaa tgtaactact ttagaagagg caaaggcctt gctggaaggg gatacagcta
     1921 ttagtgccgg ttttctggat tatgatcttt atccctggta tggttcggct gccctgcctg
     1981 agtatctcga tgctgccgat aaaatatggc gtaaaaaacc atgagggttt cttaaggtaa
     2041 agtcagagaa gtaacaagct aataatcatt aaattaatat tgtatgttgt gcacaattca
     2101 tcccaaatta ccgatgagga ataaagcggt aactgcggaa tattacatcg gcaatctggg
     2161 ttttcagcca atcggagaca gtgatttcga gggatattta atgctaaaaa aagatcagat
     2221 agagttacat ttttttgaat acaaaacact tgttccagcc gaaaattacg gacaggtcta
     2281 tatacgtacg gatgacattg ccagtttgta ccgggaactc acagacaaga atgttcctat
     2341 ccaccccaat ggtccattgg aaataaagcc ctgggggcaa atagagttct cactgctcga
     2401 tccggacagc aatctgctga cctttgggca ggggcattaa ttagcgtatg gtgaagatat
     2461 ttgaagcaac cggaaaggac atgctcatta tgaatcaaaa aagttactga tgtccggcat
     2521 cgaaggtatc aataagggat agggaattcc gatttctgta tctctaaatc ctgaattgac
     2581 tgaaaagatt gttacgcggg caaaagattg ttacaaccat taccgggaag tcaaagtaaa
     2641 cgtatgcttt cattacttca tgaatgaaat ttatggtata ttcatgtgcg gatagacaaa
     2701 tatctttttg atgttgaaag ggcctgcttc attttatacc aaaatacagg atgattttca
     2761 cagattgctt attgtttatc atatttcata gtattttcgt ttattcgaaa aaagcaaaca
     2821 atcaggataa atataaagaa atgcaaaaag taataatcga agaaggagtt gtacacgtgg
     2881 ttccggaaga tataaaagct gtgttggttg cgaatgaagc attgctggat aaatggaaca
     2941 gccttactcc tttgatgaga aatgaatgga tttgctggac caccatcgta aagcaggagt
     3001 cgacccgcaa agagcatatc cgcagattgg aagaggacat attggacgga aagaaaaggc
     3061 cctgttgctg gccgggatgc ccgcacagaa gagagagtgc caggaaatgg tttaacaaaa
     3121 tgtaactatg aaaataacgc cttttttggt ccaacatcac actcccctgt actacttata
     3181 gcagaatatt caaccactct aataaatcta attgggtgca ggcatacaat tcaaaggaaa
     3241 ggatttaaat ttttacacca tttgaaattg gctgaattct gtgagaagtc gtcagaaact
     3301 atatacttcc taatacgtta atctatatgg ataaatacaa tttatcatta catttgtaaa
     3361 ttgaaaaaaa ttttgtcgaa ctatgaaaac aattgatacg ttcatcgtac atccacaaac
     3421 gaatgaacaa gttgctgctt taaaagcctt tatgcaagcg cttaaaataa agttcgaagt
     3481 aaccaaagaa gaagaagcct acaatcctga gtttgttgcc aagatagaaa aagcaaagca
     3541 agactataaa gaaggcaaag gcaaagtata tacttctgag caactaaacg aactatggaa
     3601 gtaatcttcc tgcctgatgc tgaagaggat cttcaatatt gggtttctac cggcaataaa
     3661 gcgatcatta aaaaatagct caacttgtag aagacataaa gcttcatcct tattccggac
     3721 tcggtaaacc tgaaccacta aagtataatt tatccggtgt ttggtctcga agaattaacc
     3781 gagagcatcg cctggtgtat gaagtgactg ataatatcat ttgtattctc tctgtttggg
     3841 gacactataa gtaagtgagc aaagattgag attccgactg gaactgttta ttttattgtc
     3901 tcatagaaaa gcaatcgtct cctccatact tagggatggt cttatgtaga gaagacattt
     3961 cgatttactt gtctccgtaa tgagtataag cactcactac gattactaca acactttctt
     4021 ccttgatgcc ataaaccaat ctgtgttttc tgttgattct tctcgagtac aagccttgta
     4081 aatcatgcct gagggcttcg ggtctacctg taccggtaag gggatgttct ctcagttcat
     4141 ttaaaagttg ttctaactta ctcaatactt ttttatcccc tgccagctta tgcttttcta
     4201 tgtcctctaa tgctgttttt gtaaactcta atgtaaagct cataggccca gtaaatcacg
     4261 gatctcctct ggtgtagaca ctcgtttaac ttctccattt gaggcttcat tcaggctctt
     4321 tttaattcgg ttgaccattt cagcattaaa gtaaatatca tcttctttta tggcagttaa
     4381 catataggat ttgtctttcc cccgctgtac tattacctgc tctcccttgt caactttatc
     4441 aagatacata ctttgatttt gcctaaattc tctactgctt attactaaca taatttaaat
     4501 tttgtgtacc attatggtac aacaatgcaa atataacaat agttccttta cagcatccga
     4561 tggcccttaa ttatattcga ctttaccgac tttaaccgga caggattgta tttctcaata
     4621 atgtttagat attattgact tgtcattttc tgtcagaaat acaagatacc tctatctttt
     4681 ttgctcgctg ccggagctct ttctcccaag gtcaaaagac tccgggctcc gattaatctg
     4741 acaaagataa ctatgtagat aagaactgaa cgaactccta ataatatgtg tatgaataaa
     4801 gcagtaactt gtattacgtt atattttccg ggcagtgttc tccttaccga tttgatctag
     4861 tttattttca ttttccatta ttgcattttt ggccatcaat ttatctatta agtgcagtca
     4921 atggtgttgt tcattaactc ctactcttaa ccactgcacc cctataagtt catagtacca
     4981 caatcctcaa aggacattgt tgtgacttac ccttataaaa aattcattga tatattttct
     5041 tgagaaacct tgaagatgta ggcatgggtt gtattttatc aagtttaatt cgattgtatg
     5101 ttattaaaac acatgaatta attgtttttt gcctactccc atgatgcgga tcaatatctg
     5161 cctgattgtc aggtctttta tccatttata atgaattttc aacctcctca tttcccgaaa
     5221 ttgaaaggta atagggtccg gattatccac tcttttaaag ctgattaaaa tttttgtttc
     5281 taagaaaggg ttcatagaat atttacaaca aattattact gcgctatgaa aaaccaagac
     5341 atgatgattg tctatcctga ttatgaagaa ccaattaatg tcctgaaagt ctttttcaat
     5401 gcacaaaaga ttaagtttga accgtctaat gaaagccata cacccctgag tttggcgaaa
     5461 aaattgaaaa ggcatgacaa gactataaaa aaggaagaga caaggtttat tcttccgagc
     5521 aactcaattc aatatggaag taatctttct gcctgatgcg ggaaccgatt ttaactattg
     5581 gattgatacc aacaataaac cgattcttaa aaaaattgcc cggttcattg atggcataaa
     5641 aattaatcct tattcctgat ttgataagct taaggtaaat tatcagactt taccattgca
     5701 atcggataag cacatttaca ccttaatatg gaaccttctt ggttaagagg gtttttttat
     5761 aacaaaatca atagtaacac actaaattga actacctttg taatagatgt gattaatgga
     5821 agaattagat gaagttcaaa aaaacaattt gaaatattat tgatatcgcc actgcgatca
     5881 aacaacaaaa aaatgtgttt agcaatgtaa tgagtaccac tatgtaaaat tttcagcaat
     5941 gcatacaata gatttaattc gagagattca acgcctacca atttccaaac gtttgtatgt
     6001 ggtagaagaa accatcaaat ccattaaaaa ggaagaaatg cagcaccaaa tggaattggc
     6061 tgccaatgat ctttataatg actatgttaa cgataaggaa ttaaccgctt ttacttcact
     6121 tgatttagaa aatttttatg aagcaaagtg aagtatggtt gattgacctt gaccctacaa
     6181 aaggagctga aatacaaaag aaaagacctg caatcattgt aaataatgac agtctgggca
     6241 aactaccctt aaaagttatt gtgcctatta ccgattggaa cgaccgctat gaaattgccc
     6301 cctggatggt aaaaattgaa cccaacagca ccaatggact ttccaaaact tcatctgtcg
     6361 attgttttca aattcgttcc ttatctcaag aacggttgat taaaaaaatc ggcttcattg
     6421 atggagctac actatatgaa ataaaagaag ccattagaaa agtactggat ttataatttc
     6481 ttaaaaaaca actatgcaat cacaagaaat aatattaatt gccggttctc atgaggttgt
     6541 tgatagaagt tgaataaatt tgaacactac agaaagaatc atctatctca aaatttttat
     6601 accggccatt tcgccactcc gcctcctgaa tatagagcgg aagacgggac tcgaacccgc
     6661 cacctacagc ttaggaagct gtcgctctgc cggatgagtt acttccgcag gatgcaaatt
     6721 gccggacaat cagggacatt gccattcaat gttacacatt tctgaaaaat gaagtatcaa
     6781 ttttcgcaac caaaacagtg gaccggatat cgcaacgaaa gacttcttcc gtaaaatttg
     6841 taatcattta ttttaatata gacggtttga ttttgaagta aaggctgttt tacatactgg
     6901 tcaaacaact ataaaaattt agctaggttt cctcctgctt ccgttgcagt tcgctgcata
     6961 ttctgaaata caaacttatg gtatgcttca ggatgtcgtc ctaaatgtgg catagctgct
     7021 ttatttcttg caccgttcag gtctaaacca tattgtttgg ctatgtctcc cattttggaa
     7081 gtctaaacag aatgcttgct agtagtgatg tggtgtatct gtgttggtag tttggtactc
     7141 gccttagcgg catgttcaaa cccttctgac agtgttttaa tcgtagtttt cgaaactcca
     7201 ggtaaaaata cacctaaagc ctgatacttt gcatcagtcc aattgccgga tattatccct
     7261 tttaaagaat atacacccaa cataggcact tcggcaactt catcgacgac agcaggtata
     7321 gatgtgacca actgtcttcc gatagtctat gccaccgggt ctaaggatgt ttgctgcgca
     7381 ggacctatac ttggttgtga cccatggtac tgcggaggtc tggttacatt tctgccctga
     7441 tatgttgtag aaccaatcgt aaaagtcggc gttacgatgc cgtttttatc aggttgcatg
     7501 acatatactc tctgatggcc ccacaaatct atagagccca tcggttcatt ctccgcatag
     7561 ttatatacac tcatatgcgg atactggtca ctgaatgggc ccaccccagt aacccgcccc
     7621 agctccggta tataccaccg cgccccaaag tcagaccagt tgagccttcc cttttgggtc
     7681 cttaccccag tcgtgtagct ccttagagtt gtaaagatag ttgtttttga tgggagtggt
     7741 ggtaccgcct agcttgatac cctatttcgg ctctcctaaa atctttacag atgcatactc
     7801 ggggttttgt cctcgtggaa gagacagcat accattttat tgcggttcat cttgatgccg
     7861 tagtggtcta aaaccgtgag gatgcttaat gacttgatta tttctgctat ttccattact
     7921 gaaatttatt ttttgacaat ttgacaactg cataagtaaa acataaattc catattgaca
     7981 agtttaaaat tccatatcgt tgataatatt gtattttatg gactatttga ctaactttgt
     8041 gccgttaaat tctttattca gtatggatat agctaaaaac ataaaaaaac tccgtgaaga
     8101 acgagggttg atgcaaaagg aagtagctaa cgctgttggg gtacatcctt ccaactattc
     8161 caaaatggaa aagggcgaaa gagaatttgg gattgaggtc attgataaac tggctaaatt
     8221 cttcgggctt accattgatg aactggtaca cccaaacaac aaactaccca aagtggtaaa
     8281 agtggaagac aaaaccgcta ttgaaaaagt acagttgatt tcacagcttg atgaagaaga
     8341 taaaaacgca gtctatcgca ttattgacgg tatgctcact aaaaacaagt ttcagacttt
     8401 ctttgaacaa aacattgcag taaaataatt cagatatgct tacagtacat ccgcaataca
     8461 tcacagacaa cgcaggtaaa aaaatatctg tggtactttc tatgaaggat tttgaagcca
     8521 taatggaaga actggaagag ttggaagata taaggcttta tgacgaagcc aaaaaatcca
     8581 atgagccatc tattcctatt gatgatgcct tcaaaatgat tgaagctaaa cgcaaagcga
     8641 attaatggct tataatgtaa ccttaaaaaa acgagccatc aaagctcttg aaaaaatcaa
     8701 tgagccatat tattccaata tcaaggaagc catttacagc cttgccgata atccacgccc
     8761 tgaaggttac aaaaagctaa aaggcagaga cggttatcgt atccggatgg cggattatcg
     8821 tatcatctac gatatttttg ataacaccct actggtggat gttattgact tgggacaccg
     8881 gaaagatatt tatgagtaga acagaagccc aagcaaaaaa aagcccagga actatgtcca
     8941 gggcttcact ttttatttca cgctaccgat gtcttttcaa aatcaagacc cgttagcttc
     9001 tctttctgta tcgctttctt caatgcttca ctgacgttta ggaaaggagt aattttccat
     9061 aagttaaatg ttagctcgtc atgaaaaaca gcatacttat taatcttaga cagcgaaaaa
     9121 taaggcttca agatttggtc tgtaccggga acatattttg tatgctctct atctaaaaca
     9181 tccaactttt tctcaaatct tggaatgtaa tatgtccttt gctctgcatt agcatttacg
     9241 acagccctta tccaatctat cccctcattt cccgtcaagt gctgctcaat gacagatttc
     9301 aacctctcgg acatcaaagg ccacgcaagg ctattagact gatagtccaa ccacgtttca
     9361 cctaacccgg ccaaatcact atttacaata agtctgtctt tgccttcctt tacttttctc
     9421 aattcaaatt caaagggcaa caaagctttt ccttcaagct gagggattaa ttcatgggct
     9481 aatttcgttt cctgtaaaga aaaagcactt gccaactctg gtttatgctt ccaattcaat
     9541 gaaaaatact tactcatctt accaaccaga tttacgtaat agatttgtat cttgaaagat
     9601 gctataaaaa atcagaccac ttttagttcc ggataaaaat tttttcaaaa aaatttagaa
     9661 tactaaatag caataaatca acggattaca tcttgtcccg aaggctaaga cagaccttta
     9721 tttacatttt atttggaaat aacttcccgg atgtttatat ttgattcaac aatactcaaa
     9781 atttacaatt aaaaaacttt ctatgacaaa gccaaacact gcattttaat accttatctt
     9841 attagttgta tctgttgtaa ttgatgcatt cgctgcatca acggggagtt ttttatctgt
     9901 tttttaaaga gtttttaaat aatttatctc ttaggctcat gaagtacgtg tacctcatat
     9961 ggatatttat tgtctctatt caaggcttaa ccgcacaaga cctggagggt acctatgaag
    10021 gctttaaaag tcgctggaaa gataagcctg tcactgcaag cggcagcctc ggcttcaata
    10081 ccaactatta tgcagcgcgg catagcggca atattgccat cccctttagc tacaatctat
    10141 atggcaatgt caatctcgac atccttggaa tcaatgcacc catgggaatt ttctacgcca
    10201 ataaaaacac aaccttcaat cttccctcct accggtttat cggcatcagc cccgaattca
    10261 gagggcataa actgcatctc tttgaccgta ccatggagtt tagtccctat agtttttccg
    10321 gaacagcctt tacaggtgtc ggatacgagg gcacctttgg aaaattctat gtcaaagcaa
    10381 tggcgggacg tctcaataag gccaccatcc acgaagccaa tgtcctcaac aaccttgaca
    10441 attctttcaa aagaatagga tgggccatag gcggcggata taaagacgat aagcaacaat
    10501 acggcctcca cctgtttcag gcaaaggaca tcgcttcttc cctgccggat accttgcaga
    10561 caagggtcag gccacaggcc ggcagcattc tggtgttgga tggttcacgg cgggtgggga
    10621 tattcaagct gagcatggaa tatgcctaca atatccacac ggctgatctc aatgccgaac
    10681 tgcctgcatc gggacgcaac cttttttcca tcataccggg ctggcatccg accaacagtt
    10741 ctaccggccg ccacgatgcc tttaaatctt cccttcagat taaagcggga aaagcaacta
    10801 tcggactgga atatgagcgc atagatccgg gctacatttc attaggcacc ctttatttta
    10861 ataatgatat agaaaccatg gccctcgcat gtcagtctcc ttttttcaaa aaccggctga
    10921 caactcaggt tcggattggc cgacaaagaa ataacctgcg caatgaccag gcaaatcaaa
    10981 acgtccggct ggccttgtcc ggcaatatca acgcccggtt gacacgtaca ctcaaccttg
    11041 gatttaattt ttccaatttt tcatttaatc aaaagtccta cataaccgtc agacccgtaa
    11101 tcgacctcga caccctgata tttacccaaa ataatctgaa tactggactt tctctgacca
    11161 aacagttttc cggcaccaat cccggttcta ttcagatgat catcaactac aatgatgcca
    11221 ggacagcgag gggagattct gtcagcaatg tgatccggct aaagatgatt actgccagcg
    11281 gaggctataa taaacggttt acagactcag aaatgaacat gtatgcaggt tttaactata
    11341 acaaggtcgt ggccttattg ggcaacagcg atatgtgggg accgacgatt acggtttcca
    11401 ggtcattttt cagaaaattt tgggaatcgt ccctgagcac ttcctttctg tttgatcagc
    11461 gcgaagggaa taacaggatg attttccgga atttctggtc caacaagttg aaattttcag
    11521 agaatgccga cctggacgtc agactgatgt attctcatat caataaccgg aaggtggcag
    11581 gcacatctga gtttatgttt caaacagcct ttttattcag actcaat
//
LOCUS       DAOQQN010000045        11595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1966_length_11595_cov_5.034662, whole genome shotgun sequence.
ACCESSION   DAOQQN010000045 DAOQQN010000000
VERSION     DAOQQN010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11595
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1966_length_11595_cov_5.034662"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="PLG24_02800"
     CDS             complement(<1..1316)
                     /locus_tag="PLG24_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ23843.1"
                     /translation="MIDSNPLLSIRDLRISFETEGRVNSVIKGINLDIGKGEIVGLVG
                     ESGSGKSVTGLSLCGLLPENNARITGSIQFDHFGNAKELVGMTDNAFRQLRGSNISMI
                     FQEPMSSLNPVLTCGFQVDEMTRLHLKYNKEQARNTTLELFDKVGLPDPHRIYRSYPH
                     QISGGQIQRVMIAMAISCHPQLLIADEPTTALDVTIQKKILELLVKIRKDFGISILFI
                     SHDLGVIKDITSNINVMYRGRIVEAGSTESVLDNPQHPYTRGLIACKPPLRYKKERLP
                     VVSDFVTIEETSDDYVFKPVVKSEDRPIPKKDPVCFDQAPLLEVDNLVVKYPIASNLW
                     GKTTSWLRAVDDMSFSVYRGETLGIVGESGCGKSTLGRTIVHLEKASSGVVFFEGRDL
                     LKQRESDWKSTARQVQIIFQDPYSSLNPKMNIGAAIMEPMMVHNLY"
     gene            complement(1309..1842)
                     /locus_tag="PLG24_02805"
     CDS             complement(1309..1842)
                     /locus_tag="PLG24_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMZ23844.1"
                     /translation="MKQKTYEVLNRYSVEEFKRLKKLPVVIVLDNLRSGLNIGSAFRT
                     ADAFAVSSIELCGICAIPPNREILKTALGATESVQWRYHDSTKDCIRQLKSEGFGIFA
                     IEIATDSISLEKISFDAESKTAIVFGNEVNGISEDVLGLCDGAIEIPQLGTKHSFNVS
                     VSLGIVLWEYYRQIKND"
     gene            complement(1850..3472)
                     /locus_tag="PLG24_02810"
     CDS             complement(1850..3472)
                     /locus_tag="PLG24_02810"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HMZ23845.1"
                     /translation="MQTKFIFVTGGVTSSLGKGIISSSLAKLLQARNLRVTIQKFDPY
                     INVDPGTMNPYEHGECYVTDDGAETDLDLGHYERFLNIRTSQANNVTTGKIYQTVIKQ
                     ERAGKYLGKTVQVIPHITDEIKRRIRLLGESGEYDVVITEIGGTVGDIESLPYIEALR
                     QLRWDLGMQNSLVIHLTLIPFLSAAQELKTKPTQHSVKELQSLGIQPDILVCRTERPI
                     SQEIKQKLALFCNVEQNSVIESIDASSIYEVPLLMEKEGLDAIVLKKFNLQGLPEPNL
                     DKWKAFLSTLKYPKKEVKIALVGKYIELKDAYKSIYEAFIHAGAANECKVHVEPVHSE
                     DLLDSEVCQKVLSKMDAVLVAPGFGLRGIEGKLNAIRYVRENKIPFFGICLGMQCAVV
                     EYAKNIAGMKDAASTEVDPNTPYPVIALMEDQQHLKKKGGTMRLGAYSCKITDKSHAM
                     KAYGSALIEERHRHRWELNNQFRDKLKNAGLKITGVNPDRDLVEIIEIEDHPWFVGVQ
                     FHPELKSTVEIPHPLFVSFVEAALTHQAQNMA"
     gene            4131..5666
                     /locus_tag="PLG24_02815"
     CDS             4131..5666
                     /locus_tag="PLG24_02815"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HMZ23846.1"
                     /translation="MPKLLNSAVKVYLKLVHGMLERSITAPIEAQEKVFNKLIFNFKN
                     TKYGKLNGIKHVKNYKSYHKAYPVVTYDHLKPFIERMMKGESDVLIGGNVRFFSKSSG
                     TTNDKSKFIPVTRANLYKNHLKSAWDSLSLFYRKRPDAELFNKKSLMISGSLSHFPEY
                     PKAIVGDISAIMTKNMPTIARSFYTPDLEIAMLSDWNEKIARITEQVGSQDVVMFGGV
                     PTWLLVTFRELLKKQNKENLLEIWPNLQGYLHGGVGFSPYVEQFKALIPSKDFIYQEI
                     YNASEGFFGIQQHNSDEDMLLLVNNSVFYEFLPMSEWEKDSPEAIPLKDVETGKPYAI
                     LITSNNGLARYMMGDVVEFSSTCPYKFKIKGRTQHYINIFGEEVMVDNTDKALAMTCQ
                     ATGSSIDNYTVGPIYLTTAMKGGHEWIVEFNKKPEDINAFGQLLDNNLRTLNSDYDAK
                     RSYDLALMPLKLTIVPVGTFHRWLQSRNKLGGQNKVPRLANHRNYIDALLEFGDSLAK
                     TIT"
     gene            5672..5971
                     /locus_tag="PLG24_02820"
     CDS             5672..5971
                     /locus_tag="PLG24_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23847.1"
                     /translation="MDDLNSLTSLELPVTLPDTQVGYEEMLNAIADVVNRMLNDNPDL
                     LFSYLYRLDVDEAKIKFALSPLAETAPHIGLANLIIERQKQRIRTRQEYSEKAKK"
     gene            5984..6712
                     /gene="recO"
                     /locus_tag="PLG24_02825"
     CDS             5984..6712
                     /gene="recO"
                     /locus_tag="PLG24_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HMZ23848.1"
                     /translation="MSKLLKDKGIVVRTFKYGETSVILDILTEVHGLGSFILQGVRKS
                     KSRTSPSSIQLAASVDLDFYMKENTDLHQLKELKVNTIRTSIYSDIRKIALCMFLSEL
                     TRRMLNKYENHQLEFSLLQSVLDDLEANDDNWSIHIWYLIRLLEISGFSLLKHRVHPE
                     YYYDIPSDTITSNRPLHHSYLSGYDMITLTQLNQADVRQVSQFKPKRDERNRLVEKLL
                     DFLKVHISGFQHIQSFDVLKSVFE"
     gene            6872..7654
                     /locus_tag="PLG24_02830"
     CDS             6872..7654
                     /locus_tag="PLG24_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HMZ23849.1"
                     /translation="MLHKVTTFLSPTLGRSVKIHILETKKAIGNECLTILFNDGQDFA
                     ALRISDRIAGFELDYPNVPLRIIGLEAGDRLHEYGTMYKNDYKNRGNKAGKYNQFINN
                     ELLPYLFSRHKISLLSKMTGFAGFSLGGLSAMDIVLHNPDVFSFSGVFSGSFWWRHSP
                     FTEEDPDGSRILHEYIDTHAISSKQHFWFQTGALDEESDRNRNGIIDSIDDTVDVMLC
                     LERKGYKQGKNMTYLEVSDGTHDTGTWHRVMPDFLEWVVKSL"
     gene            complement(7813..8322)
                     /locus_tag="PLG24_02835"
     CDS             complement(7813..8322)
                     /locus_tag="PLG24_02835"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HMZ23850.1"
                     /translation="MKSLFSFLSLFLVLSVFSCKSDTTDANGQEASKAQAIADGTVSD
                     QVATGNPNEISTPTGPTTTMVLDKTEYKFSNVKEGDVVETSIKVTNTGKEPLVIVSCK
                     GSCGCTTPKCPTSPIAPGQSADIPVKFDTHGKPGTQSKDVTITANTVPAQTKFRIFGE
                     VERVPKESK"
     gene            complement(8445..10070)
                     /locus_tag="PLG24_02840"
     CDS             complement(8445..10070)
                     /locus_tag="PLG24_02840"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HMZ23851.1"
                     /translation="MENTAIVAKVKLLGELLELHDANPFKVRAWQNAYSTLKKVGEPI
                     VTMTPEQLSALPGLGKNILSAITTLVKKGSVDELDDLLARTPEGIVEMFNIKGIGPKK
                     IARFWHEMSLMSVGELIYYCTENRLRDAKGFGEKSQQDILQKAMLYESSKNKWLYANV
                     EPILLAFEDALQKASINRFRLTGQAFRREQVVDHIRYVVDSEEGILNIPEFVVLDSRN
                     DVIKGKYMDRVTIEVVIDRANPFSRAFLDSFSETVDVATLFDIDKVPDNEEESSIFKT
                     LGIPYISKELRWSNELCLLNPQNLITVEDIKGVIHTHTVYSDGIHSIREMCEFAYRNG
                     YEYIAFSDHSKSASYANGLSIERILQQHREIESMIPEFPGLTILKSIESDILSDGNLD
                     YDDEILGLFDFVIGSVHSGLNMDSERATQRLIKAIEHPFMNILGHMTGRLLLARKGYS
                     PDMAKVIDACVANNVIIELNANPQRLDLDYTQLKKATDRGVKISINPDAHSREAIMNI
                     RYGVIAGRNGGLQRENVINALPVNDFLASMVKK"
     gene            10163..10744
                     /locus_tag="PLG24_02845"
     CDS             10163..10744
                     /locus_tag="PLG24_02845"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23852.1"
                     /translation="MIIRALVGFLFIILSFQSCKEASTNAVDTSLNLDEYLVEDIPGT
                     SRKMVTKLTPQGFLEEQGMIENGKKVGTWTVFFAQNTRPMETWTYEEGKLNGLHFVYN
                     NSGRVDMYESYMNNELHGKKVTYKMGTPVEEMSYKHGKLDGVFRGFFPTGKLQRLGYF
                     KNGLQDGKYVVYDENKNIVLSATYVNGVQQPNN"
     gene            complement(10842..11243)
                     /locus_tag="PLG24_02850"
     CDS             complement(10842..11243)
                     /locus_tag="PLG24_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMZ23853.1"
                     /translation="MNEPLSGIMSTNLITVSPGDKLSVVKDLFRGKRIHHIPVVQNGK
                     LVGLITTADLLWLNRKHEEYDSILVSEIMTTKLATLEPTDKIGSAAELFLLNHFHAIP
                     VVEDGTLIGIVTSFDVLKYQFKKAYPTQLVD"
BASE COUNT         3497 a         2423 c         2204 g         3471 t
ORIGIN      
        1 ttgtacagat tatgtaccat catcggctcc ataatggcag ctccgatatt catctttgga
       61 ttgagtgaag agtatggatc ctggaatata atctggactt ggcgtgctgt ggacttccaa
      121 tcagactccc gttgcttcag taaatccctg ccttcaaaaa agacaacccc tgatgaagcc
      181 ttttccagat ggacaatggt tctccccaga gttgacttgc cacagccgga ctcgcccacg
      241 atgccgaggg tctctcctct ataaacagaa aaactcatgt catcaacagc tctgagccac
      301 gaagtcgtct taccccacaa attcgaagct ataggatatt ttacgacaag attatccacc
      361 tccaagagag gtgcctgatc gaaacatacc gggtctttct ttggtatggg tctatcctct
      421 gatttaacta caggcttgaa aacataatca tctgacgtct cttctatagt cacaaagtca
      481 cttacaaccg gcaaccgttc tttcttatat ctcaatggcg gcttgcaggc tatcagacct
      541 ctggtataag gatgttgggg attatcaaga acagattctg tagaacccgc ctctactatc
      601 ctccctcggt acatcacatt gatatttgag gtaatatcct tgattacacc aagatcatgt
      661 gaaataaaaa ggatagagat cccaaaatct ttacggattt tcaccagcaa ttcaagtatc
      721 tttttctgga ttgtcacatc caatgcagtt gtgggttcat cagcaatcaa caattgtgga
      781 tgacaactaa tggccatagc tatcatcact ctttggatct gtccaccgga aatctggtgc
      841 ggataagaac gataaatcct atgagggtca gggaggccaa ctttgtcaaa taactcaagc
      901 gtcgtatttc gcgcctgttc cttattgtac ttcagatgga gtcgggtcat ttcatctacc
      961 tgaaatccac aagtaagtac aggattcaga gatgacatgg gctcctgaaa aatcatggaa
     1021 atattacttc cacggagttg tcgaaacgcg ttatcggtca tgccaaccag ctccttagca
     1081 ttgccgaaat gatcgaattg aattgaaccg gtaatccggg cattgttctc cggtaaaaga
     1141 ccgcagaggg ataatcctgt aacggatttt ccacttccgg attctccaac aagtccaacg
     1201 atttcaccct taccaatatc cagatttatc cccttaatca cagagttgac cctgccttca
     1261 gtctcgaagg aaatcctgag atcgcggata ctaagtaaag gatttgagtc aatcattttt
     1321 tatttgtctg taatattccc acaaaacaat gcccaaactt acggaaacat taaaagaatg
     1381 tttagttcca agctgaggaa tttcaatggc accatcgcag agtcccaaaa catcttcact
     1441 gatgccattg acttcattac caaatacaat tgcagtcttg ctttcagcat caaaacttat
     1501 tttttcaagg gaaatggaat ccgtagctat ttctatggcg aaaatcccaa atccttccga
     1561 ttttaattgc ctgatacaat ccttggtcga atcatgatat cgccattgaa cagattccgt
     1621 agctcccaaa gctgttttca ggatttctct attcggtgga atggcgcaga ttccacacaa
     1681 ttcaatggat gagacagcga aagcatcagc agtcctgaat gctgagccaa tatttaatcc
     1741 ggaacgcaga ttgtccagaa cgattacaac aggtaatttc ttcaatcttt taaattcctc
     1801 gactgaatat cggttcagaa cttcatatgt tttctgcttc atcctcgggt caagccatat
     1861 tttgagcctg atgtgtcaag gctgcctcaa caaaactgac aaataatgga tgtggaatct
     1921 caacggtcga tttcaactcc ggatgaaact gaacaccaac aaaccaagga tgatcctcaa
     1981 tttcaataat ctctaccagg tcccgatccg gattaacccc tgtaatcttt agtcctgcat
     2041 ttttcaattt atcccggaat tggttgttta attcccatcg atgacggtgt ctttcttcaa
     2101 tcaaagcaga tccataggcc ttcatagcat gtgatttgtc agtgatttta caggagtacg
     2161 cccctaatct catggtcccg cctttcttct taagatgctg ttgatcctcc atcaaagcaa
     2221 tcacagggta tggtgtattc gggtcaactt cagtagatgc ggcatctttc attcctgcga
     2281 tattcttggc atactccacc actgcgcatt gcataccaag gcagataccg aaaaacggga
     2341 ttttattttc cctgacataa cgaatggcgt tcaatttgcc ttcaatgccc ctcaacccaa
     2401 aaccgggagc caccagcacg gcatccatct tactcaatac cttttggcat acttctgaat
     2461 ctaaaagatc ctctgagtgg acaggttcta catggacctt acattcattg gcagcaccgg
     2521 catggataaa agcctcataa atagatttat atgcgtcttt tagctcgata tatttaccta
     2581 cgagtgcaat tttaacctct ttcttaggat atttcaatgt tgacaggaac gctttccatt
     2641 tgtccaggtt aggttctggc agtccttgaa ggttgaattt ttttaaaaca atagcatcca
     2701 ggccttcctt ttccatcagc aatggcacct cataaataga agaagcatcg atcgattcta
     2761 tcacagaatt ctgttcaaca ttacagaata aagctaattt ttgtttaatt tcctgagaaa
     2821 ttggcctttc tgtacggcaa acaaggatgt ccggctgaat cccaagcgac tggagctcct
     2881 ttactgaatg ttgtgtaggc ttggttttca attcttgagc tgcacttaga aaagggataa
     2941 gtgtcaggtg gataaccagc gaattctgca tgcccaaatc ccaacgcaat tgtcgcagtg
     3001 cctcgatata aggcaaggac tcaatatccc ctacagttcc ccctatttcg gtaataacga
     3061 catcatattc tccggactca cccaacaatc tgatacgtcg tttgatttca tctgtaatat
     3121 gaggtatgac ctgaactgtt ttgccaagat atttacctgc ccgttcctgc ttgattacgg
     3181 tctgatatat tttaccggta gttacattat tcgcctgact tgtcctgata ttgagaaatc
     3241 gttcatagtg acccagatca agatcagtct ctgccccatc atcggttacg taacattcac
     3301 cgtgttcata tggattcata gtaccgggat caacgttgat gtatgggtcg aacttttgaa
     3361 tagtaaccct tagatttcgt gcttgaagta attttgcaag agaggaggaa ataatgcctt
     3421 tcccaagaga agaagtaact ccacccgtaa caaagataaa tttagtctgc atattcactt
     3481 gtcgttacgg actgtaaaga tatggtaaaa tacggaattc aggaggaata tttctttgga
     3541 aataaataaa gatttgggga tttagggaag attggggtgg gtgggtaatt ttaagtaggt
     3601 agataactaa tattcatcct tgagctggaa caagaaatct cagattccat ttaataaact
     3661 taagtcgagt acgggttgaa aaaaaattca gattgtttaa aaatgaggat atggtaaatg
     3721 tctcacgtat taatcaacta cctaattaga tttggacttc ccatttcatt cgatttttaa
     3781 tccataatag cattcagggc actgaccgaa attggttctc accagctgta aattaagtag
     3841 gtagataagt aatactaaaa gggggccacc ttataaggca atcaggattt aaattctgat
     3901 atttagtcaa ttaattccaa aaaaaacaat ttcactttaa gcgaagccaa tatctataga
     3961 taaattgggt attatccaac tacctaattg tttttttcct cagcaacact cctgaatact
     4021 aaaatcagat taattgaccc taagtgcatg atccaatgga ctttctccaa aaaatttgaa
     4081 caattttttt caaaatacct tatatttgag actgtttgta actgaatcct atgccaaaat
     4141 tgttaaacag tgctgtaaag gtttacttga aactggtaca cggaatgttg gagcgatcca
     4201 tcactgcacc aatagaagca caggaaaaag tgtttaacaa actgattttc aacttcaaaa
     4261 acactaaata cggaaagctt aacggcatta agcatgtcaa aaattataaa tcataccata
     4321 aggcctatcc ggttgtaacg tatgaccatc tcaaaccttt catagaacgc atgatgaagg
     4381 gagaaagtga tgtacttatc ggtggcaatg ttcgcttttt ttcgaaatca tcaggtacca
     4441 ccaacgacaa aagcaaattt attccggtaa ccagggctaa tttgtacaaa aatcatctaa
     4501 agagtgcatg ggatagcttg tccctattct accgaaaaag gcctgatgcg gaattgttca
     4561 acaaaaaatc tctaatgatt agcggctcct tgagtcactt tccggaatac cccaaagcta
     4621 ttgtaggtga tatttcagca ataatgacga agaacatgcc tactattgcc cgttctttct
     4681 acacaccgga cctggaaatc gctatgctat cggactggaa cgaaaaaatt gcccggataa
     4741 ccgagcaggt cggtagtcag gatgtagtaa tgtttggagg agtccctacc tggcttttag
     4801 tgacttttcg ggaattgctt aaaaaacaaa ataaggagaa ccttttagaa atttggccca
     4861 atctccaggg gtatcttcat ggcggagtcg gtttcagccc ttatgtcgaa cagtttaagg
     4921 ctttgattcc ttcaaaagat tttatctatc aggagattta caatgccagc gaaggctttt
     4981 tcggcatcca acaacacaat agtgatgaag acatgcttct gcttgtcaac aacagcgttt
     5041 tctatgaatt tcttccaatg agtgagtggg aaaaagattc cccggaagca attcctctca
     5101 aggacgtaga aacaggaaag ccttatgcca ttttgataac ttccaataac ggactggcga
     5161 gatacatgat gggagatgtc gtggagttca gttcgacctg cccctataaa ttcaaaataa
     5221 aaggtcgtac ccagcattat atcaatattt tcggagaaga agtaatggtg gataatacag
     5281 ataaggcatt ggcgatgact tgtcaggcta ccggatcaag catcgataac tatactgttg
     5341 gcccaatata tctaactact gctatgaaag gcgggcatga atggattgtc gagttcaaca
     5401 aaaaaccgga ggacatcaat gctttcggtc agttgctgga caataatctt cgcactctta
     5461 acagtgatta tgatgccaaa cgatcttatg atctggcttt gatgccgctt aaacttacta
     5521 ttgtacctgt tggcactttc catcggtggc ttcagtcccg taataaattg gggggacaga
     5581 ataaagtccc cagactggcc aatcatcgta attatattga tgctttgctt gaatttggtg
     5641 acagtcttgc aaaaaccata acctaaggtt gatggatgat ctgaattctc ttacttcgtt
     5701 ggaactaccg gtaacattac cggacactca ggttggttat gaagagatgc tcaatgccat
     5761 tgctgatgtt gtcaaccgga tgttgaatga caatccggat ttgcttttca gttatcttta
     5821 tcgattggat gtagatgaag cgaaaatcaa atttgccttg tcacctttag cagaaaccgc
     5881 tcctcatatt ggattggcca atctaatcat agaacgacag aagcaacgaa tccgaacccg
     5941 acaagaatat tccgaaaaag caaaaaaata aatttaatgt gcgatgagca aattgctgaa
     6001 agataaaggc atcgtcgtgc gtaccttcaa atatggtgaa acctccgtta tactggacat
     6061 tcttacagaa gtacacggtc ttggatcctt catcctccaa ggagtccgaa agtccaaaag
     6121 caggacatcc ccctcatcca tccaattagc cgcatcggtc gaccttgatt tctacatgaa
     6181 ggaaaatacc gatctgcatc aattgaagga gttaaaggtc aatactattc gtacttcaat
     6241 ttacagtgat atccgaaaaa ttgcgttatg catgtttctt tcagagctta cgagacggat
     6301 gctgaacaaa tatgaaaatc atcaattgga gttttccttg ctccagtctg tattagatga
     6361 tttggaggca aatgatgaca actggtcgat tcatatttgg tacttgattc gtttgcttga
     6421 aatatccgga ttttcattat taaaacaccg tgttcatccg gaatattact acgacattcc
     6481 ctctgacacg atcacatcta ataggcccct gcaccattcg tatctttccg gatatgatat
     6541 gattaccttg acacaactta atcaagccga tgtcaggcag gtatcccaat ttaagcccaa
     6601 aagggatgaa cgtaatcgtt tggtagaaaa attgctggat ttcctaaaag tccatatttc
     6661 gggatttcag catattcaaa gctttgatgt gttaaaatcc gtttttgaat aactccttaa
     6721 acaaaaatta aatatttcgg aagaaattga aagtattcat tcattctaat caatttataa
     6781 aacggcatat taaatgaccg gctaatctgt aattattttt tatttttgtg aaaacatata
     6841 acgaccttca tattgacttg ataaaaaaat aatgcttcac aaagttacca cttttttatc
     6901 ccctacattg ggccgatcag ttaaaatcca tatattggag accaagaaag caattggtaa
     6961 tgaatgcctt acgattctat ttaatgacgg gcaggatttt gctgcactta gaatttcaga
     7021 tcgaatagcc ggattcgaac tggattatcc gaatgtcccc ctgcgtatca ttggattaga
     7081 ggccggtgat cgcctccatg aatatggtac tatgtataaa aatgattata agaacagggg
     7141 caacaaggcg ggtaaataca atcaattcat caataatgaa ctccttccct atcttttttc
     7201 acgacataaa atctctttgt tgtccaaaat gactggattt gccggattct cactaggagg
     7261 tctttctgct atggatattg tcttacacaa tccggatgta ttctcttttt ccggtgtctt
     7321 ctcagggagt ttttggtggc gtcactcacc atttaccgaa gaagatcccg acgggtccag
     7381 aattctgcat gaatatattg acacccatgc aatcagtagc aagcaacatt tttggtttca
     7441 gacaggtgct ttggacgaag aaagcgatcg caacagaaat ggcattattg actcaattga
     7501 tgacacggtc gatgtaatgc tttgcctgga aagaaaaggt tacaaacagg gcaagaatat
     7561 gacttatctt gaagtaagtg acggcacgca cgacaccgga acttggcaca gagtcatgcc
     7621 cgattttctg gagtgggtag ttaaatctct ttaaacatcc cgggaaaggc agaatgcata
     7681 attgacctta taaaaactga ttctgaaagt atagttatct tctatattcc atggataaag
     7741 ggctgtatta aataaaaaag cagcccggat taggtcctgg ctgctttggg tttattctta
     7801 ccttttgaag gattatttag attcttttgg tacacgctct acttcaccaa aaatgcggaa
     7861 tttggtttgt gcaggaacag tattagcagt gattgtaaca tctttagact gcgtacctgg
     7921 ctttccgtgt gtatcaaatt taacagggat atcggcggac tgacccgggg cgatcggact
     7981 ggtcgggcat ttaggtgttg tgcagccaca acttccttta caacttacaa taaccaaggg
     8041 ttctttaccg gtattggtga ctttaatact agtttctaca acatctcctt ctttaacatt
     8101 agagaatttg tattcagtct tgtcaagcac catggtggtt gtagggcctg taggtgtact
     8161 gatttcattg gggttaccgg tggcaacctg atctgaaacg gttccatccg ctattgcctg
     8221 tgccttactg gcttcttgcc cattggcatc ggtagtgtca cttttacaac taaaaacaga
     8281 caacactaaa aatagtgaca gaaaagagaa taacgatttc atacgcttat ttgatttgtt
     8341 tttttgtaat ttttaataca aatataatat ggtagaatac cttccattgc taataaaagt
     8401 ctaacaagcc tttgttttac taattgttaa catagccggg ttcattattt cttgaccata
     8461 gatgccaaaa aatcattcac cgggagagca ttaattacat tctcccgttg aagcccacca
     8521 ttccttcccg caatcacccc ataccttata ttcattattg cttccctact atgcgcatca
     8581 gggttaatgc tgatcttaac tccccgatct gttgcttttt tcaattgagt gtaatccaaa
     8641 tccaatctct gtggattggc gttcaattct atgattacat tgttggcaac acaggcgtca
     8701 atgaccttag ccatatcagg cgaatatcct tttctggcaa gtagcaatcg cccggtcata
     8761 tggcctaaaa tattcataaa aggatgttca atagctttga tcagacgctg agtcgctcgc
     8821 tcagaatcca tattcaaccc ggaatgtaca cttccaatca caaaatcaaa caatccaaga
     8881 atttcatcat cataatcaag attcccatca cttaaaatat cggattcaat actcttaagg
     8941 attgtcaaac ccggaaattc aggtatcata ctttcaatct ccctatgttg ttgaaggatg
     9001 cgctctatgg acaacccgtt ggcgtaggat gccgatttgc tgtgatccga aaaagctatg
     9061 tattcataac cgttccggta agcaaattca cacatttcac ggatgctgtg gataccatcg
     9121 gagtatactg tatgtgtatg aattacccct ttgatgtcct ctaccgtaat aagattctga
     9181 ggattcagca aacacaattc gttgctccac cgcaactcct ttgaaatata tggaatgccc
     9241 aaagttttaa atatgcttga ctcttcttca ttgtcaggga ctttatctat atcgaaaagt
     9301 gttgcaacat caactgtttc ggaaaaagaa tccaaaaatg cccgactgaa tggattagcc
     9361 cggtcaataa ccacctcaat ggtaacccta tccatatact ttcccttaat cacatcattt
     9421 cgtgaatcca acacaacaaa ctcaggaata ttcaaaattc cttcttcaga atcaacgaca
     9481 tagcgtatat gatcgacaac ctgctcacgg cgaaaagcct gtccggtaag cctgaaccga
     9541 ttgatggagg ccttttgtaa ggcatcttca aaggcaagta atataggttc gacattggca
     9601 taaagccact tatttttact cgactcataa agcatggctt tttgaagtat gtcctgctgg
     9661 cttttctctc caaagccttt ggcatcccga agtctattct ctgtgcaata ataaatcaat
     9721 tcaccgacag acatcaagct catttcatgc caaaaacgag caattttttt cggcccgatg
     9781 cccttgatat tgaacatttc tacgatgccc tcaggtgttc ttgcaagcaa atcatccaat
     9841 tcgtctacag atcccttttt tactagggtt gtgatagctg acaggatatt cttacccaaa
     9901 ccaggcaatg cgcttaattg ctccggtgtc atcgtgacaa taggctctcc aaccttcttc
     9961 agcgttgaat aggcattctg ccaggctctg actttaaaag gatttgcatc atgcaattca
    10021 agcagttctc cgagtagttt gactttagcg actattgctg tattttccat catgttagga
    10081 ttcgaatatt atgaattcat tgacgcacca aaaaccgtta tttaaaccgg gtatttaata
    10141 aatttgcgca aaatataaaa aaatgataat tcgtgcatta gtaggatttc ttttcatcat
    10201 tctttcattc caatcctgca aggaagcatc cacaaatgct gtagacacct ctctcaatct
    10261 tgacgagtat ttggtagaag atattccggg tacaagtaga aagatggtaa ctaagttaac
    10321 tccacagggc tttctggaag aacaaggcat gatagaaaat gggaaaaagg tgggtacctg
    10381 gaccgtattc tttgcgcaaa acacgcgtcc gatggagact tggacctatg aagaaggaaa
    10441 actgaatggg ttgcattttg tttataacaa ttcaggtagg gtggatatgt atgaaagtta
    10501 tatgaataat gaactccatg gtaaaaaagt aacctacaaa atgggtacgc ctgtggaaga
    10561 aatgagttac aaacacggaa aactggacgg tgtcttcaga ggtttcttcc ccaccggtaa
    10621 gcttcaacga ctgggttact ttaaaaatgg cctacaggac gggaagtatg tagtatatga
    10681 tgaaaataag aatattgtac tatcagcaac ttacgttaat ggcgtacaac agcctaacaa
    10741 ttagcgctga ctaaccaaca tcataaatat gaaaagaggc caacatcctt agggaaacag
    10801 ggaaatacct gcatcagcct ttgagtcagt caatcgactt atcaatcaac caattgggtc
    10861 gggtacgcct tcttgaactg gtacttcaat acatcaaagg atgtaacgat acctatcaat
    10921 gtaccatctt caacgaccgg gattgcatga aaatggttaa gtaaaaataa ctctgctgca
    10981 cttccaattt tgtctgtagg ttccagggtg gcaagttttg tagtcattat ttcacttaca
    11041 agaatggaat catattcctc atgcttccta tttagccaaa gcaaatcagc tgtcgtaata
    11101 agccccacta atttaccgtt ctgtactacg ggtatatggt gaatcctttt tcctctaaac
    11161 aaatctttca ccacagataa cttatctccc ggacttacag tgattaggtt ggttgacatt
    11221 attccggata acggttcatt catcatgact ataattctta tttgagtgta atattaacca
    11281 ttttttttct gccatgataa acctaaataa tatatttatt ttacacaact atatttatct
    11341 ttaaatctga tatttatgtc gtaaaattca tgaataataa aaattgaata attggtatta
    11401 tgtaaaccat gttttatcaa taatccatga gtagtgttaa agtttttgac taattttaca
    11461 tatattttta atctgaaaag atgcatgatt tagagcctta ttatcaatgg cgggattatt
    11521 atatcgcctc tgaagacaag ctttcgccct attatgggca cgaatctaat gagttcgagt
    11581 ttgtcaataa ggtat
//
LOCUS       DAOQQN010000046        11544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1978_length_11544_cov_5.745322, whole genome shotgun sequence.
ACCESSION   DAOQQN010000046 DAOQQN010000000
VERSION     DAOQQN010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11544
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1978_length_11544_cov_5.745322"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PLG24_02855"
     CDS             <1..429
                     /locus_tag="PLG24_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ23854.1"
                     /translation="IYNGENTSAPLIGKLCGSKLPGNIIADNPSGALTFLFKTDPLNA
                     YQGWHAKVTCVERSATEQMIHADIRLFPNPAGNVLNISMGAPMSGNIIITDLLGNIIR
                     NRELRGENLIQLSTDGLHSGVYFASIRFGKNLITRKFIVE"
     gene            446..1471
                     /gene="galE"
                     /locus_tag="PLG24_02860"
     CDS             446..1471
                     /gene="galE"
                     /locus_tag="PLG24_02860"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HMZ23855.1"
                     /translation="MSKILVTGGCGYIGSHTIVELIESGFEVVCVDDLSNSDGSALQG
                     IKAITGKDVTFYNYDAGDINRMQEVFTREGRIDGIIHFAAFKAVNESVQLPLKYYVNN
                     VSGLANLLQTALKNGCNRFLFSSSCSVYGDADQLPVSESSPLGEIRSPYARTKLICEE
                     ILMDLASSGSPMKSIALRYFNPAGAHPSNEIGESPTINASNLVPVITETAIGKRPGFT
                     VFGQDYDTRDGTCIRDYIHVSDIAAAHALAMKLLLENHYTLPFDIYNLGSATGNTVLE
                     VIDAFVRVNEIRPNHVIGPRRAGDVVAVYADNSKAREQLSWKLRYNLDDIVRTAWNWE
                     KRRSGQN"
     gene            complement(1646..2035)
                     /locus_tag="PLG24_02865"
     CDS             complement(1646..2035)
                     /locus_tag="PLG24_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23856.1"
                     /translation="MKQSILLGMVILLYMVFSGGCTKDNMLCTDDGKFCRSIVNSGEE
                     KALAAINSYLSKQETNQADSTKLHLLKDWLECKSCIQEVRILCNSCIETYPTQSELSV
                     RLTGSEPDNVLVLDILMSKPLKAIRFH"
     gene            complement(2113..2829)
                     /gene="pyrH"
                     /locus_tag="PLG24_02870"
     CDS             complement(2113..2829)
                     /gene="pyrH"
                     /locus_tag="PLG24_02870"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HMZ23857.1"
                     /translation="MELPYKRILLKLSGESLMGGQGFGIESERLEHYATEIRSAIDLG
                     VEVAVVIGGGNIFRGLQAHNSGIERVTGDYMGMLATVINGMAVQSMLETKGVYTRLLS
                     ALEIREVAEPYIRRRATRHLEKGRVVIFSAGTGSPYFTTDSAAALRANEISADVLLKG
                     TRVDGIFSSDPEKDPTATRYSEITFDEVINKNLNVMDMTAFTLCRENSLPIIVFDINT
                     PGNLARLLKGEKVGTLVRNR"
     gene            complement(2951..3787)
                     /gene="tsf"
                     /locus_tag="PLG24_02875"
     CDS             complement(2951..3787)
                     /gene="tsf"
                     /locus_tag="PLG24_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HMZ23858.1"
                     /translation="MSVEISASAVKQLRDTTGAGMMDCKKALTEANGDFEAAIEILRK
                     KGQKLSLKRADRDAKEGVVIAQVSADGKKGIVIKLSSETDFVAKNEDFIALAKKFANI
                     ALETFPATKEDLLALPFEGITIGDKVTEQVGVIGEKIELADYEQLEAEQVVPYIHSGY
                     RAGVIVGLNKANGQSAEAGEDLAMQVAAMKPVAVDKDGVDAETIAKEIEIGKEVARAE
                     GKPEDMLEKIAMGKLNKFYQESTLLNQAFVKGDGKKSVRDYLASIDKDLTVTGFRHVA
                     LG"
     gene            complement(3835..4563)
                     /gene="rpsB"
                     /locus_tag="PLG24_02880"
     CDS             complement(3835..4563)
                     /gene="rpsB"
                     /locus_tag="PLG24_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HMZ23859.1"
                     /translation="MEKPSYKELLDAGVHFGHLKRKWNPKMHPYIFMERKGIHIIDLN
                     RTADCLENAAGAMKQMAKSGKKILFVATKKQARDIVSEAAKSVNMPFVTERWLGGMMT
                     NFATIRRSVKKMHNIDRMLSDTTLTSVTKKERLLMGREKVKMERVFGGVASLNRLPSA
                     VFVVDVGHEHIAISEAHKLGMKTFAMVDTNADPNQVDFPIPSNDDASKSISLIVNYMT
                     NAIKEGLEERKAMKQDNEGEEEDA"
     gene            complement(4618..5004)
                     /gene="rpsI"
                     /locus_tag="PLG24_02885"
     CDS             complement(4618..5004)
                     /gene="rpsI"
                     /locus_tag="PLG24_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HMZ23860.1"
                     /translation="MEIINAIGRRKSSVARIYLSKGEGKITVNGKDYKEYFPQGHIQL
                     KVVEPTGTVEASGIYNIKVNVRGGGIKGQAEAIRMAIARALVKIDEEFKKPLKSKKLL
                     SRDSREVERKKYGKPKARKSFQFSKR"
     gene            complement(5009..5452)
                     /gene="rplM"
                     /locus_tag="PLG24_02890"
     CDS             complement(5009..5452)
                     /gene="rplM"
                     /locus_tag="PLG24_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HMZ23861.1"
                     /translation="MNTLSYKTVSAKKETVEKKWFVIDAENEVVGRLCSKIAHVLRGK
                     HRPDYTPHVDCGDYVIVINADKVRFTGSKWQQKEYIRYSLYPGGQKRVVAEKMHENQP
                     VKVIETAVKGMLPKNKLGNAMYKKLFVYAGDSHNHAAQKPETLKF"
     gene            5945..6586
                     /locus_tag="PLG24_02895"
     CDS             5945..6586
                     /locus_tag="PLG24_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23862.1"
                     /translation="MEYDQSKYKVRTWKNPMMLHWIVNPGLAFNELVLGQRVPKIMLI
                     ERNDSKSLQEKTFVPCPHCGTIHSGQKWSVENNAFKNWFGLYCDACGKIIPCLRNITS
                     WVLMTLTYPLWFWLKDSRKSRWLERQPVRYKNLNLTNQPSPYEGRGWIRQGLYWGLLM
                     WIMMAVIFPLIDGSGITVKNLLIGIPVWAVGGLGFGYTMKLIMGKGRASSQSI"
     gene            6975..7595
                     /locus_tag="PLG24_02900"
     CDS             6975..7595
                     /locus_tag="PLG24_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23863.1"
                     /translation="MINLIKNVLFIIVLCGCNSPSNTQTVKVTCDQLGLYFMLPSDFT
                     SLDSDKIESLEKEGNKAIKEAFNKKALKGWQPSCLNLQDSYKSTILVSSISTEEAVEL
                     NGSIDEFIEKTFKDSNDFIARRFKQKANIDIDLNESVVQTRMTIAGYKVKKNAFTFKY
                     GGMLMYFSRYYFFLRDDKLYLLGYTGSPKADNNDEIVNAIENAKLL"
     gene            7623..7919
                     /locus_tag="PLG24_02905"
     CDS             7623..7919
                     /locus_tag="PLG24_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HMZ23864.1"
                     /translation="MTRYKPRHYLIEGQDGGPYSSRSLQNIFTAYPEKAGITTHTTVH
                     TLRHSYATHLIPGGKDIRIVQELLEHRSLKTTEIYTHIVKRRKDIKSPVGSLGI"
     gene            8005..8322
                     /locus_tag="PLG24_02910"
     CDS             8005..8322
                     /locus_tag="PLG24_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23865.1"
                     /translation="MKEIKTSKDGIDYMTAVLFEKPLTVTRAMLWKIKHNNSENDELA
                     VKLGRYKKKKGPFNLTFEELETSIPKSELTLIGEEFQALISFIESKFNPFEQGVKKFI
                     PLD"
     gene            8485..8979
                     /locus_tag="PLG24_02915"
     CDS             8485..8979
                     /locus_tag="PLG24_02915"
                     /inference="COORDINATES: protein motif:HMM:NF025448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4263 domain-containing protein"
                     /protein_id="HMZ23866.1"
                     /translation="MLENDMTEHDWQKWFQENNWVLGTDYVRILDERNIDTKNITDFL
                     MEAYDGFIDIVEIKRPDGNLNFWAKSKDHDNYVPDQDLVKAITQATKYIYEIERETNS
                     IKFLERVDGVKTIKPRCVLIYGRSDDWNNEQKESFRILNSNYHNLSIMTYDHILERAR
                     RMIK"
     gene            9363..10211
                     /locus_tag="PLG24_02920"
     CDS             9363..10211
                     /locus_tag="PLG24_02920"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ23867.1"
                     /translation="MGNVKKPTTMTFENHKISSPLDKYIESIFYYKDFVPEHNIEKVI
                     PTGNIFILIELDGLERKTFDNKLEPIGHFSKSWISGIHQKHLNISAHKNSEMLVIQFK
                     SFGAYPFLKLPINELNNKVVQAEKYFGDSILKLREAIILKQSISDKFKIVEEWLLGIF
                     DENSILPKEIMDFMKTLQSQPFSKHNELLKDYPKTSKNLIDNVKKYCGLTPKVFHRIF
                     RFNTLLANINQKKDIVWTDIVYETGYSDQSHFIKEFQEFSGFNPTQFIKNGYNDSVPN
                     FLPLDK"
     gene            10281..10688
                     /locus_tag="PLG24_02925"
     CDS             10281..10688
                     /locus_tag="PLG24_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HMZ23868.1"
                     /translation="MDISILNWFAVIVAALSNFLIGGLWYSPILFGKVWMKENNFSDD
                     DLKKGNMPKIFGLTFLFSIVMAFNLAMYLNDASTTASWGAIAGFLAGFGWVAMSIFTI
                     GQFERKSAKYMLIHGGYVTISFIVMGLIIGLLR"
     gene            10698..11249
                     /locus_tag="PLG24_02930"
     CDS             10698..11249
                     /locus_tag="PLG24_02930"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HMZ23869.1"
                     /translation="MDKATQTMIDNLHKNTGKSLEQWIEIVKSQNFVKHGEVVKFLKE
                     QHEFTHGFANLVAHKANESDAGSAENQDDLITKQYSGKEHLKVFYDKLISEIQTFGND
                     IEIAPKNSYVSLRRKKQFATLNPATKTRFEIGINLKGQEPKGKLEPEKPNAMCSHKIN
                     LTDIKDIDNEVIEWISIAYNNAG"
     gene            complement(11318..>11544)
                     /locus_tag="PLG24_02935"
     CDS             complement(11318..>11544)
                     /locus_tag="PLG24_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23870.1"
                     /translation="PTYWNTVHSVHCGTAMPSIGDGKCYFPSMTDKSQPSIRQKDKNP
                     SKRDTLHNPVSLDAILSLSKYTEYSKARLA"
BASE COUNT         3563 a         2305 c         2347 g         3329 t
ORIGIN      
        1 atatacaatg gagaaaacac atcagctccg cttatcggaa agctgtgcgg atcaaaattg
       61 cccggcaata ttattgcaga caatccatcc ggcgcgctta cctttctctt caagacggac
      121 cctttgaatg cataccaggg ctggcatgct aaggtaacct gtgtagagcg gtctgctacg
      181 gagcaaatga tacatgcaga catccggtta ttccctaatc cggcaggtaa tgtgctgaat
      241 ataagtatgg gagcaccgat gtccgggaat atcatcataa ccgacttact tggaaatatc
      301 atcagaaaca gggaattaag gggagagaat ctcattcagt tgagcactga cggactacat
      361 agcggagtat attttgccag tatccgtttc ggcaaaaatt tgatcaccag aaaatttatc
      421 gttgaataaa cgtataggtc tatctatgtc gaaaatactc gtcaccggcg gatgtggcta
      481 tataggcagt cacacaatcg tagagctcat cgagtcaggt tttgaagtcg tatgtgttga
      541 tgatttaagt aattccgatg gaagtgccct acaggggata aaagcaatca ccggaaaaga
      601 tgtcactttc tataattatg atgcaggaga tatcaaccgg atgcaggagg tttttacacg
      661 ggaaggaaga atcgatggca tcatccactt tgcagcattc aaggcggtca atgaatccgt
      721 gcaattgccc cttaaatact atgtcaacaa tgtatcgggc ttggccaatc tcctgcagac
      781 agctctgaaa aatgggtgca accgcttcct gttttcatcc tcgtgcagcg tctatggcga
      841 tgccgatcaa cttccggtct cggaatccag tcctcttgga gaaatacgca gtccctatgc
      901 acgaacaaaa ttgatctgtg aagaaatcct gatggatctg gcatcttcag gttcacctat
      961 gaaatccatc gcgttgcgtt atttcaatcc tgccggtgcc catccctcta atgaaatcgg
     1021 cgaatcgccc accatcaatg cctcaaatct tgtaccggtc ataactgaaa ctgctatcgg
     1081 caagagacct ggttttacgg tctttggtca ggattacgat accagggacg gcacttgcat
     1141 ccgtgattac attcatgttt cggacatagc agcagcacat gccctggcga tgaagctcct
     1201 gctggaaaat cattatacac tacctttcga tatctacaac ctgggctctg caaccggtaa
     1261 taccgttctt gaagtcatcg atgcctttgt tcgggtcaat gaaatcaggc ctaatcatgt
     1321 catcggacct cggcgtgccg gtgatgtcgt ggcagtatat gccgacaatt caaaggccag
     1381 ggagcaacta agctggaagc ttagatacaa tcttgatgac attgtacgta ccgcatggaa
     1441 ctgggaaaaa cgaagaagcg gacaaaattg atgtggagtg agaagtttga aaagtgaaaa
     1501 agataccttt atgccaatag gtgatatatg gatctaatac ggtgtgtttt gatcccgaaa
     1561 ggcgacttac atcagtcaaa actgcaatcc atgactttta ttcgtaacca taatttgcac
     1621 tgtgcccata tccgggaaaa ctaaatcaat gaaacctgat agccttcaac ggcttcgaca
     1681 taaggatatc cagcaccagc acattgtcag gctctgaacc tgtcagcctg accgacaatt
     1741 cgctttgagt agggtatgtt tcaatgcagg aattacacaa tatccggacc tcctgaatac
     1801 aactcttaca ctccagccaa tctttgagca agtgtagttt ggtgctgtcg gcctgattcg
     1861 tttcctgctt gctcaaataa ctgttgatgg cagcaagagc cttttcttca ccggagttga
     1921 cgatagaacg gcagaatttg ccatcatcag tacaaagcat gttatctttt gtgcacccac
     1981 cggaaaacac catgtaaagt aaaatgacca ttccaagcaa tatcgactgt ttcataatca
     2041 caaatatcct ttattatagg aacgatcaat cttgaaacct ggttggaaat gtcgttaaga
     2101 aaaacctgca cattaccggt ttctcaccag tgtacctact ttttctcctt tcagcagtcg
     2161 ggcaaggtta ccgggagtat taatgtcgaa aacgatgatc ggcaatgaat tttcacggca
     2221 cagggtaaat gccgtcatat ccatcacatt gagattttta ttgatcactt catcgaaggt
     2281 gatttcagaa tatctggtag cagtcgggtc tttttcggga tctgaagaaa agatgccgtc
     2341 caccctggtg ccttttaaca agacatcagc actaatctca ttggcccgca aagcggctgc
     2401 tgaatcagtt gtaaagtatg gactaccggt cccggcagaa aaaataacca ctctaccctt
     2461 ctccaaatgg cgtgtggcgc gtcgtcggat gtaaggctcc gccacctctc tgatctccag
     2521 agcagacaat aatctcgtat agacaccttt agtctccaac atactctgca ctgccatacc
     2581 attgatgacg gtggccagca tccccatata atcgccggta accctttcga tacctgaatt
     2641 atgggcctga agacctctga aaatattacc accaccgatg acgactgcca cttccacacc
     2701 cagatcgatg gccgacctga tttccgtggc ataatgctcc aatcgctcag attcaatgcc
     2761 aaatccctgt ccgcccatca atgactcacc gctcagcttc agtaatattc tcttataagg
     2821 caactccatg tcaagatttt tttaaactaa aaagaacaaa atgataaaat ggggagacga
     2881 aactttcaaa acaggatgca aacaggtata aaaaaagcga atggagttcc attcgctttt
     2941 ttcaattttc ttaacccaga gctacatgtc tgaagccggt caccgtcagg tctttgtcga
     3001 tggaggcaag ataatccctt acggatttct ttccatcacc cttcacgaat gcctgattga
     3061 gcaatgtgct ctcctgataa aatttattca gtttgcccat ggcaattttt tccaacatgt
     3121 cttcaggctt accttcagcg cgtgcaacct ccttaccgat ctcgatttcc tttgctatgg
     3181 tctcagcatc gacgccatcc ttatccactg caacgggctt catggctgct acctgcattg
     3241 ccaggtcttc accggcttct gcagattgac cgttagcttt gttcagaccc acgattacgc
     3301 cagccctgta tccactgtga atataaggaa caacttgttc agcttccaat tgctcgtaat
     3361 cggccagttc gatcttctca ccgataacac ccacctgttc tgtcacctta tctccaatgg
     3421 taataccttc gaaaggcaag gccagcaagt cttccttggt tgcagggaat gtttccaaag
     3481 ctatattggc aaacttcttg gcaagagcga taaagtcttc gttcttggca acgaagtccg
     3541 tctcggagct cagtttgatg acgattcctt tctttccgtc agcacttacc tgagcgatga
     3601 ctacgccttc cttggcatcg cggtctgctc tctttaatga aagtttctga ccttttttac
     3661 gcaggatttc aatggcagct tcaaaatcac cattagcttc ggtaagggct tttttacagt
     3721 ccatcattcc tgcaccggtt gtatcgcgga gttgcttcac cgcacttgct gatatttcta
     3781 cactcatttt atgattgatt tataaaaatt aatgaatcaa ggcagctgag gaaattatgc
     3841 atcttcctct tcgccctcgt tatcctgctt catagccttt ctctcctcca gaccttcctt
     3901 gatagcattg gtcatataat tgacgatgag agagatggac ttggaagcgt cgtcattgct
     3961 tgggattggg aaatcaacct gattgggatc cgcattggta tccaccatgg cgaatgtctt
     4021 catgccaagc ttgtgagctt ctgagatagc gatgtgctcg tgtccgacat ccacaacaaa
     4081 taccgctgat ggtagtctgt tcaggctggc gacaccaccg aacacacgtt ccatctttac
     4141 cttctcacgt cccatcagca aacgctcctt cttggttacg ctggtcaagg ttgtgtcgct
     4201 caacattcta tctatgttgt gcatcttttt aacagatcgt cggattgtgg caaagttggt
     4261 catcatacca cccagccaac gctcggttac gaaaggcata ttgacactct ttgcagcctc
     4321 agagacgata tcacgtgcct gcttttttgt cgcaacaaac agaatcttct tgccggactt
     4381 agccatctgt ttcatggcac ctgcagcatt ctcaagacag tctgctgttc tgttcaggtc
     4441 aatgatatgg atacccttgc gctccatgaa gatataagga tgcatcttag ggttccactt
     4501 tcttttgaga tgtccgaaat gcactccggc atccagtaat tctttatatg atggtttttc
     4561 cattgattta tatttatact ataatggttt gtataatctt tttgataaaa acaggcatta
     4621 acgcttactg aactggaagc tctttcttgc cttaggctta ccgtatttct tacgttccac
     4681 ctctctggag tctctggaca acagtttttt agacttcaaa ggctttttga attcttcatc
     4741 gatcttgacc aatgccctgg caatcgccat tctgatggct tcagcctgac ctttgatccc
     4801 gcctccacgt acgttgacct tgatattgta gatacctgaa gcctcaacag ttcctgtcgg
     4861 ttctaccacc ttcaactgga tgtgtccttg tggaaaatat tctttataat ccttcccgtt
     4921 gacggtgatt tttccttcgc ctttggaaag gtaaattctt gctacggaag attttcttct
     4981 tcctattgcg tttatgattt ccatttactt agaattttaa tgtttctggt ttctgtgcag
     5041 cgtggttgtg tgaatcacct gcatagacaa ataacttctt atacatggca ttgcccaatt
     5101 tatttttagg caacataccc ttcactgcag tctcaatgac cttcacaggc tggttctcgt
     5161 gcattttctc ggcaaccacc ctcttctgac ctcccggata caaagagtac ctgatatatt
     5221 ccttttgctg ccacttggaa ccggtgaagc ggaccttatc agcattgatt acaatcacgt
     5281 aatcaccgca atcgacatgg ggagtgtaat ccggacggtg cttacccctc aatacatgag
     5341 cgatcttgct acaaagacgg cctaccactt cattttccgc atcgatgacg aaccattttt
     5401 tttcaacagt ctctttttta gctgaaaccg tcttataact taatgtattc acgacttgta
     5461 tttatttttt gttgaataat gaggcgcaaa ggtaaatctt attttgatat gcaaatagct
     5521 ttttgtaaat ttttttgaaa aatattttta taaatagctg aaaaacagtg caaaaatcga
     5581 catataaaat taattatacg ataattcttc agaaaatcta agatttatac ataaaaaata
     5641 ataatatatg atagatcata aattgtaaac aaatagcagc tgggtttatt atccggatac
     5701 accgctgcag tagtgaacat gaagcggact ccatcctctt tttcgccctg aacatacggc
     5761 tgaggtgaag gtaaatcgga agtgagagtc gtgatgaaac taagggtgta taaaaaattt
     5821 tcatgtaaag aatgtggcgt tggaagggcg ttgatgtgta tatttatgag aaaattaaag
     5881 tacatcaggc ggcttcaagg cataatatga ttcaatctgg aaaaacgaaa taaacaccat
     5941 aaatatggaa tatgatcaat caaaatacaa ggtccggacg tggaaaaacc cgatgatgct
     6001 ccactggata gtcaatcccg gccttgcatt caatgaactg gtactggggc agagagttcc
     6061 gaagattatg ctgatagaac ggaatgattc aaaaagtctg caggaaaaaa cgttcgttcc
     6121 ctgtccgcat tgtggcacca tacactccgg gcaaaaatgg tctgtcgaaa ataatgcatt
     6181 taaaaactgg tttggcctgt actgtgatgc atgtggcaag atcattccct gcctcaggaa
     6241 tataacgagc tgggttttaa tgacattgac ctatccgttg tggttttggc tgaaggacag
     6301 ccggaaaagc aggtggctgg aaagacagcc ggtgaggtat aaaaacctta accttaccaa
     6361 tcagcctagt ccgtatgaag gaaggggatg gatcaggcag gggctatatt ggggattgct
     6421 tatgtggatt atgatggctg ttatctttcc attgatagat ggaagcggca taacagttaa
     6481 aaacctattg atcggaatcc ctgtctgggc agtcggcggg ctcggattcg ggtacaccat
     6541 gaagctgatt atggggaagg gcagggcgag ttctcaatcc atatgaatgc agtcttctga
     6601 tgaggatttc ccgatcggaa aaatcatccg ggcgaaatca tagtgaatca ttttttgtcg
     6661 gctgtcgatg tgacaggcag agatgtattt cctgttaaat ttcttggcat cgtacaggac
     6721 gcgggggact tttttaggta gctgagtttt tcggcagggc tgcgacccgg ggagattatc
     6781 cgactcagga aggatgaagt aaatgaagat acttattgta ttttgatcag atgtgctaag
     6841 gggagaagga cggtattgca gcgctttcgg agaaaataag ggctgatcag gtttaaaaaa
     6901 tatcagcaaa aacagactgt ataatctcgg ctttatatta ttaattttgc attcaaaagc
     6961 tcaagagata aatcatgatc aatcttatta agaatgtcct cttcatcatc gtattatgtg
     7021 gatgtaattc accaagtaat actcaaactg taaaagtaac ttgtgatcaa cttggattgt
     7081 attttatgtt accgtcagat tttacttcac ttgactctga taaaatagaa tcactggaaa
     7141 aagaaggcaa taaggctata aaagaagcct ttaataaaaa ggctctgaaa ggatggcaac
     7201 cttcttgttt gaacctgcag gattcttata aaagtacgat tctggtaagt tctatcagta
     7261 cagaagaagc tgtggaactg aatggttcca tcgatgagtt tattgaaaaa acatttaagg
     7321 atagtaacga ttttatagcc cgacgcttta agcaaaaagc aaatatcgac atcgacctaa
     7381 atgaatcagt tgttcaaacc cggatgacaa ttgcaggata caaagtaaaa aagaacgcat
     7441 tcacttttaa gtatggtgga atgcttatgt atttttcacg atattatttc ttcctgaggg
     7501 atgataagct atatttatta ggttatacag gatctcccaa ggcagacaac aatgatgaga
     7561 tagttaatgc gatagaaaat gcaaaattgt tgtaatgctg taatcatttc aacagctgat
     7621 tcatcaccag atataagccg aggcattatc tgatagaagg tcaggacggc ggtccatatt
     7681 cgtccaggag cctacaaaac atcttcacgg cctatccgga aaaggccggt attacgacac
     7741 ataccacggt gcacaccctg cggcatagct atgctacaca tctgatccct ggcggaaagg
     7801 atatccgcat tgtacaagag cttttggagc atcgctcact gaagacgacg gagatttata
     7861 ctcatatagt taagaggaga aaggatataa aaagcccggt gggtagtttg ggtatttgag
     7921 aaaaaggatt acttttatat gtggtattta agccatatgg cgtaaataca attgttagcg
     7981 gtcagcttaa aagacgaaac gtaaatgaaa gaaattaaaa cgagtaaaga cggaattgat
     8041 tatatgacag cggtactttt tgaaaagccg ttgacagtca ctagagcaat gctttggaaa
     8101 ataaaacata acaattcaga aaatgacgag ttggctgtta aattaggacg atacaaaaag
     8161 aaaaaaggac catttaactt gacatttgaa gaattagaaa cgtcaatacc aaaaagcgaa
     8221 cttactctta taggcgaaga atttcaggca ctaataagtt ttattgaaag taaatttaat
     8281 ccatttgagc aaggtgttaa aaagttcatt ccacttgact aacaatttga agcggggaat
     8341 ttagagcatt tacaagcttt ctttagtcac cctgaaaaac aagaattaat tgattttgta
     8401 ttagggcacg acattattcc tgatgaactt ttcgtagcat tcaatcaact atcaaaaatt
     8461 agagcagttg aaaagttcga agaaatgctt gaaaatgata tgactgagca cgattggcag
     8521 aaatggtttc aagaaaacaa ctgggtttta ggaacagatt atgtgagaat tcttgacgag
     8581 agaaatattg atacaaaaaa tattactgat tttttaatgg aagcttatga tggatttatt
     8641 gacattgtag aaattaaaag accagatgga aacttaaatt tttgggctaa aagtaaagac
     8701 cacgacaact atgttccaga ccaagattta gtaaaggcaa ttacccaagc aacaaaatat
     8761 atttatgaaa ttgagcgtga aacaaatagt attaaatttt tagaacgagt tgacggagtt
     8821 aaaaccataa agccacgatg tgtattaatt tatggtaggt cagacgattg gaataatgaa
     8881 caaaaagaga gttttagaat tttaaactca aattaccata atttaagcat aatgacttac
     8941 gaccatattt tagaaagagc aagacgaatg ataaaataaa gccgaaccgc taacattaag
     9001 ccgaatatac aaaagcccac caaaaatctt ccaacttaag ccactaagtc agccgggctt
     9061 ttgaatattc ggtgttcagc gagaccgtat gatggactgt atttcactta ggtggatttc
     9121 tatctttttt gccggatgga tggctgactt ttgtcagtca aaggagaaag atagcacttg
     9181 gctaccggag aaattaccat attcttagcg aaagctttat atttatcact aaaataaaat
     9241 caaagcaaaa attgattctc cctataggca tgatcgcctc gtccaacttg aaattttgat
     9301 acttgacatg catcactttt tagggttgaa atggggaggg tatcaatatt cctttgagtt
     9361 ttatgggtaa tgttaagaaa ccgacaacaa tgacatttga gaatcataaa atatcatcac
     9421 cattagacaa atacattgaa agtattttct attacaagga ctttgttcct gaacataata
     9481 ttgaaaaggt aattccgaca gggaatatat ttattcttat tgaacttgac ggattagaaa
     9541 gaaaaacatt tgacaataaa cttgaaccca ttggacattt tagtaaaagt tggatttcgg
     9601 gaattcatca gaaacatttg aacatttcag cacataagaa ttccgaaatg ctagtcattc
     9661 aatttaaatc ttttggagca tatccctttt taaagttacc gataaatgaa cttaacaata
     9721 aagttgttca agctgagaag tattttggtg atagtatttt gaaacttcga gaagcaatta
     9781 ttctaaaaca aagcatttca gacaaattca agatagttga agaatggttg ttaggtatat
     9841 ttgacgagaa cagcatactg ccaaaagaaa taatggactt tatgaaaaca cttcaaagtc
     9901 aacccttttc caaacacaat gaattactga aagactatcc caagacgagt aaaaacctca
     9961 ttgacaatgt taaaaaatat tgtggtttga caccaaaggt ttttcataga atttttcgtt
    10021 tcaacactct attggcaaac atcaaccaaa aaaaggatat agtttggact gacatagttt
    10081 atgaaacggg ttattccgac caatcacatt ttataaagga gtttcaagag tttagtggtt
    10141 ttaatcctac tcaatttatt aaaaacgggt acaatgattc cgttccgaat tttttgccac
    10201 ttgacaagta ggttaatttt ctacaataca ttgattaaac tacgaagtaa atttgcacca
    10261 aatttaaaac gatattgatt atggacatat caatacttaa ttggtttgca gttattgtag
    10321 cagcactttc aaatttttta attggcggcc tttggtattc accaatactt tttggcaagg
    10381 tatggatgaa agagaacaac ttctctgatg acgacctaaa aaaagggaat atgccaaaga
    10441 tttttgggct tacatttctt ttctcaatag taatggcgtt taacttggca atgtacttaa
    10501 atgatgcaag tacaactgca agttggggtg caattgcagg atttttagca ggatttggat
    10561 gggtagctat gtctattttt acaatcgggc agtttgaacg gaaatctgca aaatatatgc
    10621 tcattcacgg tggatatgtt accatcagtt ttattgtaat gggattaata attggacttt
    10681 tgagataaaa tcatactatg gataaagcaa cgcaaacaat gatagataac ctgcataaaa
    10741 atacaggcaa gtctttagaa caatggattg aaattgtgaa atctcaaaat tttgtgaaac
    10801 acggagaagt tgtaaaattt cttaaagagc aacacgagtt tactcacggt tttgcaaatc
    10861 ttgtagcaca caaagcaaac gaatcggacg caggttcagc agaaaatcaa gacgacttga
    10921 ttactaaaca gtattcagga aaagaacatt tgaaagtatt ttacgataaa cttatttctg
    10981 aaattcagac tttcggaaac gacatagaaa tcgcaccgaa aaattcttac gtgagtttaa
    11041 gaagaaaaaa gcaatttgcg actttgaacc ctgcaaccaa gacgcgtttc gagattggta
    11101 tcaacttaaa aggacaagag ccaaaaggga aattagaacc tgaaaaacct aacgcaatgt
    11161 gttctcataa aataaatctt actgacataa aagacattga taatgaagta attgagtgga
    11221 taagtatagc ttataacaat gcaggataga agaaccacta cccataaaat tatgccgaat
    11281 atacaaaaga ccgtcaaaaa acttccaaat taagccacta agccaatcgg gcctttgaat
    11341 attcggtgta cttcgacaag ctcagtatgg catccagcga gaccggatta tggagggtat
    11401 ctctcttaga tggattctta tctttttgcc ggatggatgg ctgacttttg tcagtcatag
    11461 aaggaaaata gcacttgcca tccccaattg aaggcatggc agtgccacag tgcacggagt
    11521 gcactgtgtt ccaataagtt ggat
//
LOCUS       DAOQQN010000047        11460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_1999_length_11460_cov_4.878036, whole genome shotgun sequence.
ACCESSION   DAOQQN010000047 DAOQQN010000000
VERSION     DAOQQN010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11460
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1999_length_11460_cov_4.878036"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLG24_02940"
     CDS             complement(<1..649)
                     /locus_tag="PLG24_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23871.1"
                     /translation="MANTLDIIRASFIWGTYITTPGKPVSGHSLNELPYLENGFISIV
                     DGRIRRIGLMSEYSPVAGRTVMDASGAHILPAWCDSHTHLVHAASREKEFEMRLRGKS
                     YEEIAAAGGGILNSADVLRAMEEEELFDLSYRRFQAVQKLGTGAIEIKSGYGLDFDSE
                     VKMLRVIRRLKEKNEIPVKSTFLGAHAFPREFKDNHPGYISLIIDRMLPYIADHGL"
     gene            complement(649..1656)
                     /locus_tag="PLG24_02945"
     CDS             complement(649..1656)
                     /locus_tag="PLG24_02945"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HMZ23872.1"
                     /translation="MRLSIILFLFLTGAGISSAQSPSYFEMSYLEAKQKASKSNKLCA
                     VVFTRDLSKSSAYFTEVAFEDPEVKKAVTDSYLIAASNVRDFDSKSLLKKWGLDQAPS
                     IAIVNAKGDVVGTANYGMSKSELARFLQYYSIPANAGKKSSMDDGGFEGEVAAWEGFK
                     GKDMDGKMAVNNPVTAASEAKMGIVPAATQEKPVENVQPPKDVEKEAIAVRGEVPVVA
                     ENNQAVAEKPKQEPKAVAPQTHTQPATQAKNPEPTHHDAGEYKFLVQAGIFSVETSAR
                     TLVSKIEAQGGNAYIEPVVQPDKTVYKVIAGKYYTEKEARDFIAKLKDAGIASFIKPV
                     N"
     gene            complement(1799..2434)
                     /locus_tag="PLG24_02950"
     CDS             complement(1799..2434)
                     /locus_tag="PLG24_02950"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HMZ23873.1"
                     /translation="MFSGKKIFILAPHTDDGELGCGGTIARAIEEGAEVYYIAFSTAE
                     QSVPKEFPSNQLEVEVREAVTLLGIRPEHLFVYKYEVRKLNYVRQEILELLMELKKRI
                     NPDIIFVPSMRDIHQDHSTVAQEAIRAFKTKTIYGYELIWNNIDFRTNAFIVLEQRHI
                     DQKINALSAYHTQAGKDYMDPDFIRSLARVRGTQIGEVYAEAFELVRTVIS"
     gene            complement(2615..3709)
                     /gene="wecB"
                     /locus_tag="PLG24_02955"
     CDS             complement(2615..3709)
                     /gene="wecB"
                     /locus_tag="PLG24_02955"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HMZ23874.1"
                     /translation="MKKVYFVVGARPQFIKAAPILVAMRQYPVAMKLIHTGQHYDYNL
                     SDIFFDELEMPKPDYNLDCGSGTSTEQYLSVLSRLTQVLMHDRPDLIVVFGDTNSTGA
                     AALSARFNGIAVAHVEAGLREFDKNIPEEINKLVADALADLYFCPSTTAVEQLEGCKA
                     DGRIIHSGDPVIDLLVTGSKYTDRPELPAEYGLTPGKYIFATCHRQSNTNHQSALENV
                     LRIFANAHLPVLFPVHPRTMKAIHRFGLEHYLRDKNIKVIEPIGFWVTQALISQCERV
                     LTDSGGIIKEAYFHGKYVLVLDNQTEWVEVIREGKGIVCGTDYKMAIDNLKNKPDTSS
                     PNTALGDGTASIKIAKGIMEFLEGIHSPSV"
     gene            complement(3706..4968)
                     /locus_tag="PLG24_02960"
     CDS             complement(3706..4968)
                     /locus_tag="PLG24_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23875.1"
                     /translation="MPMQDKKILFLLYNFPPEFGTAPKRNFRMYKAIGKHFSQSFIIT
                     RKKQESRAEHEIIELKSYDYRDLLKRFSTSGYVSESAKSSLFSRLLIKLVNTFPFNIL
                     IGEGGGIYLWHTCKAASRLIRQHSITHIYSSYRPIADHFVGNRLKKKFPHLVWIADFR
                     DLPVEPIYKLQFFPHFHHRLYHRLFCRVDVMTTISQGLADQLKRYDRPVFSVMNGVDV
                     DFRFPQPNTTNYFTLVYTGSMYSDERNPWPVFAVLNDLIREGKVDRDKIRIEYAGKDS
                     AFWREMAQKAQLGDILEDYGLVGGDQAGRLQQNACINLLLTMASDQLSGILTGKYIEY
                     LQAGSPILCIVKNQHDDFLTQELSRLNAGLSVSDNEEEYPVIGQFILEKYLSWQVTGM
                     NEKSSNQSAILQHYDSDRLISAIEPYLK"
     gene            complement(4952..6085)
                     /locus_tag="PLG24_02965"
     CDS             complement(4952..6085)
                     /locus_tag="PLG24_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMZ23876.1"
                     /translation="MPYKVALVANSTWNFFNFRLNIIRSLKERGYEVVLISPPDQYLA
                     HLLEVVEVKHIPLNSLDRKSVNPFGEMALYREFKRIYRNEAPDLILHYTIKPNIYGNM
                     AAKKLGIHSICVITGLGYVFLHDNIYNSVAKRLYRKSLHKADLVVFENAEDKDLFVQL
                     ALAPAEKTSVVNGCGINLEYFKPISYPEKSSAKTVFTFIARLILDKGIMEFIEAAKSV
                     RSRYPDVEFWVAGEIDEGNPTSISHETLYEWVESGVIKYFGHVRDVRPIIAKSDCIVL
                     PSYREAMARTLTEALAMEKPVISCDTGGCRTIVDDGVNGYLVPVKDVDALVHAFETFK
                     LLPEISREKMGKSGREKAISEFDEEIVTSQFIKLIDGLNAHAG"
     gene            complement(6100..7176)
                     /locus_tag="PLG24_02970"
     CDS             complement(6100..7176)
                     /locus_tag="PLG24_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HMZ23877.1"
                     /translation="MKNNHYIAIMAGGVGSRFWPASREARPKQFIDMLGMGRSLLQMT
                     YDRFTPLVNPENIFCVTNQAYVNLVSQHLEELPPANIIGEPSRNNTAPSVLLTTLLIA
                     RENPDAVIGFVPSDHLIVKDQHYREICTEAFDFAQKNDAIVTLGIHPGRPDTGFGYIN
                     YNKKCTTGHIHKVISFREKPDLATAEQFIQSGDYLWNAGMFFMSIRTLLHNYKLHAPA
                     LYDMLTPFLSAGEEKVPGLIKELYPATPNLSIDYAIIEKAEDVYTIPSDIGWSDLGTW
                     NSLFQQVEKDDSGNAVMCNHIILENVSNTLVRNNTDKLVILRDVKDLIIVEEEDVLLI
                     YPMNKEQEIKQLGDKVRAKFEQYL"
     gene            complement(7186..7752)
                     /locus_tag="PLG24_02975"
     CDS             complement(7186..7752)
                     /locus_tag="PLG24_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMZ23878.1"
                     /translation="MKTPNPEGYHVVLTTHNSRTSSRMIKHGIFAGPALLLNLEQEIL
                     LTRCIGQVIIESGFRCIAYNVCRDHVHLIIVCQPEHLTGIIQKIKSVSSKNFRRNLPR
                     GQDPLDGHHPLSHSHLWSQKFYRANLDVWNLNTLTSQSGYIYKSTHLENAICYIINNR
                     KKHKLPESLELEEVISTFVVSQELAFEK"
     gene            complement(8131..8754)
                     /locus_tag="PLG24_02980"
     CDS             complement(8131..8754)
                     /locus_tag="PLG24_02980"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HMZ23879.1"
                     /translation="MLQTQTVEPGTLDLLKKCMELDALQPFTLVGGTALSLQYGHRMS
                     IDLDFFGNVNFLDDKLISEQFASIGNAELVNQSSVMLGFFINEIKVDIVKYKYDFIEK
                     PITEGIIRLAHPKDIGAMKLAAISGRGKRKDFYDLYYLLRDYSLEDLISFNKKKFPDS
                     NEMIIIKSLGYFDDADLDQQPILMEQVSWSTVKKTISGHLQNYIRKK"
     gene            complement(9086..10009)
                     /locus_tag="PLG24_02985"
     CDS             complement(9086..10009)
                     /locus_tag="PLG24_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HMZ23880.1"
                     /translation="MIKTSKIYVAGHNGMVGSAIVRKLRKVGYDNIVVRTSAQLDLRN
                     QAAVDAFFEEEKPEFVFLAAAKVGGIVANNTYRAEFLYDNLMIESNIIHAAYQHGVDK
                     LLFLGSSCIYPRIAPQPLREEYLLTGPLEPTNEPYAIAKIAGIKLCEAYRDQFGCNFI
                     SAMPTNLYGPGDNYDLEKSHVIPALLSKIHHAKVHGDATVEIWGSGNPRREFLHVDDL
                     ADACFYLMHNYEERQFVNIGCGEDISIRELAELIGKIVGFTGQFVFNTDRPDGTPRKL
                     MDVSILRKRQWSPAISLEEGLRSVYEERFLK"
     gene            10262..11443
                     /locus_tag="PLG24_02990"
     CDS             10262..11443
                     /locus_tag="PLG24_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ23881.1"
                     /translation="MKNFTTTLLFLLISTCIFSQTKHNISFMLDGHMRESIVVIPSHA
                     PPATGYPLVMMLHGTSGDGEKFYKISGWKELGEQENFVTVFPSSLTWCFVDDGVEKFN
                     TRWVNGNVTENPCSGAPQDYVDDVKFLKLLVSMIKDSVPINANKVFISGFSNGCAMIH
                     KMANEAGDVFAAAAGTSALLARSDSSRPVNRIPLWNVVGNQDDRFFFPPYTEVPFGRD
                     SILDYLKPYFVRVLACQGLSDNFTFSQTPLSHTYTFTESRGQEQSKPYIFSLLKGMTH
                     EYPNGVNFPISAPEIFWNFFNQVTSDVNPSGNLPEANWSIYPNPAVNELHIIIPGFDP
                     VKGYSVMIRDMEGRTVLTKTGIRDRGTTLTLDSLIPGVYVVHLISGHYSSARKLIVLK
                     E"
BASE COUNT         3154 a         2565 c         2323 g         3418 t
ORIGIN      
        1 ccagaccatg atcagcgatg tatggcaaca tcctgtctat aatgagtgaa atatatcccg
       61 ggtggttgtc cttgaattca cgaggaaagg catgcgcacc aaggaaagtt gacttgacag
      121 gaatctcgtt tttctccttc aggcgacgaa tgacacgtaa catctttacc tcgctgtcaa
      181 aatcaagacc atacccactc ttgatctcaa tcgctccggt acccagtttt tgcactgcct
      241 gaaagcgccg gtaactaagg tcgaaaagtt cttcttcttc cattgctctg agcacgtcag
      301 ccgaattgag gatacccccg cctgcagcgg ctatttcttc ataagatttt ccacgcaggc
      361 gcatctcgaa ttccttttcc cggctggcag catgcaccag gtgagtatga ctgtcacacc
      421 atgccggcag aatatgggca cccgatgcat ccataaccgt acgtcctgca acaggtgaat
      481 actcggacat cagacctatt ctccggatcc tgccatccac gatgcttata aagccgttct
      541 ccaaataagg aagctcattc aggctgtgtc cggaaaccgg cttcccgggt gttgttatgt
      601 acgtccccca tatgaagctt gcccgaatga tatcgagagt atttgccatc aattgacagg
      661 tttgataaag gaggcgatac cggcatcttt cagtttagcg atgaaatcac gtgcctcctt
      721 ctcggtataa tatttaccgg caattacctt atagacagtc ttatcaggct gaacgaccgg
      781 ttcgatatat gcattacccc cctgagcttc aatcttggac accaatgtcc ttgcactggt
      841 ctccacactg aaaattcccg cctgaaccag aaacttatat tctccggcat catgatgtgt
      901 cggttcggga tttttcgcct gtgttgcagg ttgcgtatgt gtctgcgggg caactgcctt
      961 tggttcctgc tttggctttt ctgctactgc ctgattattt tcagcaacaa cgggtacctc
     1021 tcctcttaca gcgatggctt ccttttcaac atccttcggg ggttgaacat tctcaaccgg
     1081 cttttcctga gttgcggcag gcactatgcc catcttcgcc tcagaagcag cggtaacagg
     1141 gttgttgacc gccatcttgc catccatgtc tttgcctttg aatccttccc aggcagcaac
     1201 ctctccttca aatccaccat catccattga ggatttttta ccggcgttgg cagggatgct
     1261 ataatactgc aagaacctag ccaattcgga ttttgacatg ccataattgg cagttccgac
     1321 aacatcacct ttggcattga caatggcgat agaaggcgcc tgatccagac cccatttctt
     1381 gagcagagat ttgctatcaa agtcacgtac attggaggca gcaatgagat aactatcagt
     1441 taccgctttc ttgacttcgg gatcttcaaa agctacttca gtaaaatagg ccgaactttt
     1501 gctcagatct ctcgtaaaaa caacggcaca taacttatta ctcttagatg ctttttgctt
     1561 agcttccagg taactcattt caaaataaga aggagactgg gctgacgaaa tgcctgcacc
     1621 tgtaaggaaa agaaacaaaa taatggagag acgcataatg tggttttata aaaaatggtc
     1681 aaattccgtt ccgaaaacaa ttcggtatca agtgcaaata taaattaccg gattgattta
     1741 aagaaaattt taacaaatat attacatata tattaaatat gtaattttca aataatcttt
     1801 atgatataac agttctgacc aattcaaatg cttcggcata gacctctccg atctgtgtgc
     1861 cgcgtaccct tgccagtgac cgtatgaaat cggggtccat ataatcttta cctgcttgcg
     1921 tatgataggc acttaatgcg ttgattttct gatcaatatg tctttgttcc aggacaataa
     1981 aggcattggt ccgaaaatca atattattcc aaatcaactc ataaccgtat atggttttgg
     2041 tcttgaatgc gcgtattgct tcctgcgcca cggtactgtg atcctgatgg atatcgcgca
     2101 tactcggtac aaagatgata tccggattga tccttttttt caactccatc agcaattcaa
     2161 ggatttcctg acgaacataa ttcagtttgc ggacttcata tttatataca aataaatgct
     2221 ccggcctgat gcctagcaaa gtaactgctt ctcggacttc tacctccagt tgattggaag
     2281 gaaactcctt tggaactgac tgctcagccg ttgaaaatgc aatataatat acttcggctc
     2341 cctcttcaat ggcccgtgcg atggtgccac cacagcccaa ctcaccgtca tcggtgtggg
     2401 gagcaaggat gaatattttc tttccggaaa acatggtatt aacaaagata attaaaataa
     2461 cagaaactga aatagcgtga atttgttttg aagcatcatg atttgtactt atcaggccac
     2521 ttcaggtata atggaaagtt tgaaaaattg aggatcaaat gtctgacaat aatgacctgt
     2581 cctttggcat agtcactttg gtcttcagta aatattacac tgaaggactg tgtatcccct
     2641 ccagaaattc cattattcct tttgcgattt tgatcgaggc agttccgtca cccaaagcag
     2701 tattgggaga ggatgtatcc ggcttgtttt ttagattatc tatggccatt ttataatcag
     2761 tgccacatac aataccttta ccttccctga ttacttccac ccattccgtc tgattgtcca
     2821 gaacaaggac atattttcca tggaagtaag cctccttgat gataccgccc gaatccgtca
     2881 atactctctc acattgactg atgagggcct gtgtaaccca aaagcctatt ggttcgatca
     2941 ccttgatatt cttgtcccgg agataatgtt ccagcccaaa tctatggata gctttcattg
     3001 tcctgggatg aaccggaaag agaaccggca ggtgggcatt ggcaaagatt cgcaatacat
     3061 tttccaaagc cgattgatga ttggtattac tttgcctgtg gcaggttgca aaaatatatt
     3121 tgcccggtgt cagaccatat tcagccggca attcaggcct gtcagtgtat ttgctgccgg
     3181 taacaagcag gtcgatcacc ggatctccgc tatggatgat ccttccatcc gccttacatc
     3241 cttccaactg ctctactgcc gttgtgctcg ggcagaaata cagatcagcc aaggcatctg
     3301 ccaccaattt gtttatttct tccggaatgt tcttgtcaaa ttccctcaat cctgcctcta
     3361 catgggccac agcaatccca ttaaatctgg cgcttaaggc tgctgcaccg gtcgaattgg
     3421 tatccccgaa tacaacaatc agatcaggcc tgtcatgcat caacacctgt gtcaaccgcg
     3481 acaggacact caggtattgc tctgtggaag ttccggagcc acaatccagg ttataatccg
     3541 gcttaggcat ctccaattca tcaaaaaata tatcggacag attataatca taatgctgac
     3601 cggtatggat cagtttcatc gccaccggat attgtcgcat tgccaccagg ataggtgcag
     3661 ccttgataaa ctgtggcctg gcacctacga caaagtaaac ttttttcatt tcaaataagg
     3721 ttcaatcgct gagatcagcc ggtccgaatc ataatgttgc aggatggccg attggttgga
     3781 agatttttca ttcattcctg tcacctgcca ggacagatat ttctctaaga taaattgccc
     3841 gatgacagga tactcctctt cattgtccga aacacttaat cctgcattta atcttgataa
     3901 ttcctgtgtc aggaaatcat catgctgatt tttaactata cacaatattg gactgccagc
     3961 ctgcaggtat tcgatgtatt ttcccgtaag gatgccactc aactgatcag acgccatggt
     4021 gagcagcagg ttaatacagg cattttgctg gagccggcct gcttgatctc ctccgaccag
     4081 gccataatcc tccaggatat cacccaattg agccttttgc gccatttcac gccagaatgc
     4141 actgtcttta ccggcgtatt caatccgaat cttatcccga tcaacctttc cctccctgat
     4201 tagatcgttc aacactgcga aaaccggcca tggattgcgc tcatcagaat acatcgaacc
     4261 ggtatagacc aaggtgaaat aatttgtcgt attgggttga ggaaatctaa agtcgacatc
     4321 cacaccattc attactgaaa aaacaggcct gtcatagcgt ttcagctgat cagccagacc
     4381 ttgggagata gttgtcatta catctaccct gcagaatagt ctatgataca ggcgatgatg
     4441 aaaatgagga aaaaattgaa gtttataaat gggttctacc ggtaaatccc gaaagtcggc
     4501 gatccaaacc aaatgaggaa atttcttttt gagccgattg ccgacaaaat gatcggcaat
     4561 gggacgataa gaagaataaa tatgcgtaat agaatgttgt ctgatcagtc gggatgctgc
     4621 tttgcaagta tgccaaaggt agatgccccc accttcaccg atcaggatat tgaacggaaa
     4681 agtattgact aatttaataa ggagcctgct gaagaggctg cttttggctg attctgagac
     4741 atagccggag gttgaaaacc tttttaaaag gtcacgataa tcataggatt tcagctcaat
     4801 gatttcatgt tcagcccgac tttcctgttt cttcctggtg atgataaatg attgactgaa
     4861 atgcttgcca atagccttat acatccgaaa gttgcgcttc ggtgccgtgc caaattcggg
     4921 agggaaatta tagagaagga aaaggatttt tttatcctgc atgggcattc aagccatcga
     4981 ttaatttaat aaactgtgaa gtaactattt cttcgtcaaa ttcggaaatt gccttttctc
     5041 ttccactttt acccattttt tcacgactga tctcggggag caatttgaac gtttcaaatg
     5101 catggaccaa agcatcaaca tccttgacag gcactaaata tccatttact ccgtcatcta
     5161 caatcgttct gcaaccaccg gtgtcgcagg aaatgacagg cttttccatt gccaatgctt
     5221 ctgtcagtgt ccgggccatc gcctcccggt atgagggaag gactatgcaa tctgatttcg
     5281 caataatcgg ccttacatcc ctgacatggc caaaatattt gatgacgccc gactcaaccc
     5341 attcgtagag tgtctcgtgg ctaatcgatg ttggatttcc ttcatcgatt tcaccagcca
     5401 cccaaaactc gacatccgga tagcggctcc taacagactt tgccgcctca atgaattcca
     5461 tgataccttt atccaggatc aaccgcgcga taaaggtaaa tacggtcttg gcagaagact
     5521 tctccggata gctgataggt ttaaaatatt ccaggttgat tccacaaccg ttgaccaccg
     5581 atgtcttttc tgccggagcc aatgccagct gcacaaagag atccttatcc tctgcatttt
     5641 caaatacgac gaggtccgcc ttgtgcaggg atttacgata aagtcttttt gccacactat
     5701 tgtaaatatt gtcatgcaaa aagacgtatc ccagcccggt tatgacgcaa atcgagtgga
     5761 tgccgagttt cttcgccgcc atattgccat aaatgtttgg cttaatggtg tagtgcaaga
     5821 tgagatcagg agcctcgttt cggtaaattc ttttgaattc gcgatacaga gccatctcac
     5881 caaaaggatt gacacttttt ctatccagac tattgagggg aatatgcttc acctccacta
     5941 cttcaagcag atgggccaga tactggtcag ggggtgatat cagcaccact tcatagccac
     6001 gttccttcaa ggatcgaata atattcaacc ggaaattgaa aaaattccag gtagaattgg
     6061 ctactaaggc aaccttgtat ggcattttat ctttcaattt tataaatatt gttcaaactt
     6121 agcacgaacc ttgtcgccta attgtttgat ttcctgttct ttattcattg gataaatcaa
     6181 caatacgtct tcctcttcca caataatcaa atccttgaca tcccttaaaa ttaccaattt
     6241 atccgtattg ttccttacta aagtgttgga gacgttctcc agaatgatgt gattgcacat
     6301 gacggcattg cccgagtcat ccttttcaac ctgttgaaac agagagttcc aggtacccag
     6361 gtctgaccag ccaatgtctg acggtatggt atagacatct tccgccttct ctatgatggc
     6421 atagtcgatg gacagattgg gtgtagccgg ataaagttct ttgattaaac cgggcacctt
     6481 ctcttcccct gctgacagga aaggtgtcaa catgtcgtaa agagcgggtg catgaagttt
     6541 gtaattatgc aacaaagttc tgatgctcat aaaaaacatg ccggcattcc acaggtagtc
     6601 tcccgattgg ataaactgtt cggcagtggc aaggtcgggt ttctcccgga aggaaatgac
     6661 tttgtggatg tgacctgtcg tgcatttttt gttgtagttg atgtatccaa agccggtgtc
     6721 gggacggcct gggtggatac caagggttac gatggcatca tttttctgag caaaatcaaa
     6781 ggcttctgta catatttccc ggtagtgctg atccttgacg atgaggtggt cggacgggac
     6841 gaatccgatg acggcatccg gattttctct ggcgatcagt agggtggtca gcaggacgct
     6901 cggggctgta ttgttgcgcg acggctcgcc gatgatgttg gcgggtggca attcttccag
     6961 gtgttgactg acgaggttta cataggcctg attggtgacg cagaagatat tctctggatt
     7021 gaccaggggt gtaaaccggt cataggtcat ctgtaacaac gatcgtccca taccgagcat
     7081 gtcaataaac tgtttgggac gtgcttctct gctggcaggc cagaacctgc tgccgacgcc
     7141 gcctgccatg atggctatgt aatgattatt tttcatgctt tttatttatt tttcaaatgc
     7201 cagttcctga ctcaccacaa aggtcgaaat tacttcttcc aattccaatg attcaggcaa
     7261 tttatgtttt tttctgttgt tgatgatata acaaatggca ttttcgagat gtgttgactt
     7321 atatatgtat ccagattgac tggtcagcgt atttaaattc cacacgtcaa gattcgcccg
     7381 ataaaatttc tgcgaccaaa gatgactgtg ggagagtggg tgatgtccat ccaaggggtc
     7441 ctgacccctt ggaaggttac ggcggaaatt tttactcgag acagatttta ttttctgtat
     7501 aatcccggtc aaatgttccg gttggcagac aatgatcagg tggacatggt cccggcacac
     7561 attataggcg atgcagcgaa aaccactttc aatgataacc tgaccaatac aacgtgtcag
     7621 caaaatctcc tgttccagat tgagtaataa agccgggcct gcaaatattc catgctttat
     7681 catacgggat gacgtacggc tgttgtgggt agtgaggacg acatggtagc cttcagggtt
     7741 tggggttttc ataaatgtta aaatttaggg aagttactta taaatattca atttgtacaa
     7801 atttagaaaa ttttgacaaa tactatcctt tattttttca gaatagttta aaacaaatta
     7861 ctcttttctg aattttaatg ccggttcgac aatggatcaa ttaaaaagct taacgctcga
     7921 tatttaaggc tctactttat aactcttcgt tatttttggg caattcatcg tctcattcgt
     7981 taaatttaac aagcctgtca gagggcttac atccgacgga aatttgtttt ttatttgtga
     8041 aatatatcat acaaaaaatt ccaggttgca tcatctgaaa tcagattttc acctatttgt
     8101 tgggcatgta gaaattctga tggttgagaa tcattttttg cggatataat tttgaagatg
     8161 gcctgaaatc gtctttttaa cagttgacca tgatacttgt tccattaaaa taggctgttg
     8221 gtcaaggtca gcatcgtcaa agtatccaag actttttatt attatcattt cattactatc
     8281 agggaatttt ttcttattaa aactaatcaa atcttccagg gaataatccc tcaaaagata
     8341 gtaaaggtca taaaagtctt ttcttttgcc tctacctgaa attgctgcta atttcattgc
     8401 tccaatatct tttggatgag caaggcggat aattccttct gttataggtt tttcgatgaa
     8461 gtcatattta tatttcacta tatcgacttt aatttcatta atgaaaaaac ccaacataac
     8521 actagattga tttacaagtt cggcgtttcc tatagaagca aattgttctg aaataagttt
     8581 atcatcgaga aaattaacat tgccaaaaaa atcaaggtca attgacattc tatgaccata
     8641 ttgtaaggaa agtgctgtac cgcctacaag ggtgaatggt tgaagggcat ccaactccat
     8701 acattttttc aataaatcca gcgtccctgg ttcaactgtc tgtgtttgta acatctgaaa
     8761 tcagattttt gtaatccgga aagtatcacc aaaaatgata aagtcttcgg gctgagatat
     8821 cttattcctc tgcttatttc aatgatatta tcaacgttat aaacctgttt tataatttgc
     8881 caatcgttcc aatttccata ctccaatact ctttcgatta taaaagcagc attttggtcc
     8941 cactgaagat cttctttttt cgtatcccag aagatttgac tggaaaggtc aattatattg
     9001 taatttgtag tcattcttga gatttggtaa gatatacttc aaagataaca aaaattatac
     9061 ttaaaaaatt acctgcaatt agtcgctatt ttagaaacct ctcctcatac acacttctca
     9121 atccttcttc cagactgatc gccggactcc actgcctttt tctcagaata cttacatcca
     9181 tcagctttct gggagtgccg tcagggcggt cggtattgaa gacaaactga cctgtaaatc
     9241 ctactatctt accgatgagt tcggcgagct ctctgatgga gatatcttca ccgcaaccga
     9301 tattgacaaa ttgtctttct tcataattgt gcatcagata aaaacaggca tctgccaggt
     9361 catccacatg gagaaattcc cggcgcggat tgccgcttcc ccatatctcc accgtcgcgt
     9421 caccatgcac cttagcatga tgtattttgc tgagcagggc agggatgacg tgtgactttt
     9481 caaggtcata attgtcgccg gggccgtaaa gattggtcgg catggctgaa ataaaattgc
     9541 atccgaactg atcacgatag gcctcacaca gcttaattcc ggcaatcttg gcaatggcat
     9601 acggctcatt ggtaggttcc agcggaccgg tcaacagata ctcttcccga agcggttgag
     9661 gcgcaatcct gggatagata cacgaactcc ccagaaacaa caacttatcc acaccgtgtt
     9721 gataagcggc gtggatgatg ttggattcta tcatcaggtt gtcgtataaa aactcagcac
     9781 gataggtatt gttggcaacg atgccaccca cttttgccgc tgcgagaaaa acaaattccg
     9841 gcttctcttc ttcaaaaaat gcatcgacag ctgcctgatt ccgaagatcc agttgagcac
     9901 ttgtcctgac gacgatgttg tcataaccaa cctttctgag tttgcgcaca atagcagaac
     9961 cgaccatccc attgtgtccc gcgacatata tttttgaggt tttaatcatg aaatattttt
    10021 tgtttaaatt gactttcaaa ataaatttga aatatatgaa aatttgaaat attcaatcca
    10081 gggatatcta gtaccaagtg gattattgaa taaatattaa tttcctgcta aaatataatc
    10141 tatttcctgt agaaaaaaag ttaaaatgaa gtatcagtaa tataattgtt cttaattaaa
    10201 aaactattta attgatatat tttcaagtat tattgttaat tttgaacaaa aatcattcgc
    10261 catgaaaaac tttactacaa cactcctctt ccttctgatt tcaacctgta ttttttcaca
    10321 aacgaagcac aacatcagtt ttatgctgga tggacatatg cgagaaagta tcgtcgtcat
    10381 tccgagccat gcgcctcctg ctaccggtta tcccctggtc atgatgctcc atggtaccag
    10441 tggtgacgga gaaaaatttt acaagatctc cggttggaaa gaactgggag aacaggaaaa
    10501 ttttgtcact gtatttccat cttccctcac ctggtgcttt gttgatgatg gggtagaaaa
    10561 atttaacacc agatgggtca atggtaatgt taccgaaaat ccttgttccg gagcaccaca
    10621 ggattatgtg gatgatgtca aatttttgaa attattggta tctatgatta aagactcagt
    10681 gcctatcaat gccaataagg tatttatcag cggtttttcc aacggttgtg ccatgatcca
    10741 caaaatggcc aacgaggccg gagatgtctt tgcggctgcc gccgggacca gcgccctatt
    10801 ggcccgatca gattcttcgc ggcctgtcaa tcggattccc ctttggaacg ttgtcggaaa
    10861 tcaggatgac agatttttct ttcctcctta tacggaagtg ccttttggaa gagattccat
    10921 tctggattat ttaaaaccat attttgtccg ggtattggca tgtcaggggc tttctgataa
    10981 ttttaccttt tcacaaactc ccctaagcca tacctataca tttaccgaaa gccgggggca
    11041 ggagcaatcc aagccctata tattcagcct tctcaagggc atgacacatg aatatcccaa
    11101 tggcgtcaat tttccgatta gtgctcctga aattttctgg aatttcttta atcaggttac
    11161 ttctgatgtt aatccttccg gtaatttacc tgaagcaaac tggtccattt atcccaatcc
    11221 tgccgtcaat gaactccaca tcattattcc cggattcgat cccgtcaaag gatattccgt
    11281 aatgataaga gatatggaag ggcggactgt attgactaaa accggcatcc gtgacagagg
    11341 aactacttta acgctggatt cattaatccc gggagtttac gtcgtccacc tcatatcagg
    11401 tcattattct tctgccagaa agttgattgt tttaaaagaa taaccaagcc aattcctgta
//
LOCUS       DAOQQN010000048        11420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2005_length_11420_cov_5.228157, whole genome shotgun sequence.
ACCESSION   DAOQQN010000048 DAOQQN010000000
VERSION     DAOQQN010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11420
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2005_length_11420_cov_5.228157"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..1084)
                     /locus_tag="PLG24_02995"
     CDS             complement(206..1084)
                     /locus_tag="PLG24_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HMZ23882.1"
                     /translation="MAELNVRSISKELKAGIGKEVADIVASGARPPHLAAIMVGDNPA
                     SKAYVGNKVKTCEELGIKSTLLHFETGITQEELVEVIRRLNADDEVDGFILQLPLPDH
                     LDPQALLLEIDPNKDVDGFHPMNFGKMALSLPAFLPATPYGIVMLIEYLGLDLSGKKV
                     AVLGRSDIVGTPISLLLSRKAPYGNATVTLLHSRSKNIKEELLAADVIVAAIGIPHYV
                     KEDMVKEGSTIFDVGINRIPDPMAKSGSRLVGDVDFEQVKDKAAWITPVPGGIGLMTI
                     VSLIRNTLDAYKNRRN"
     gene            1553..2035
                     /locus_tag="PLG24_03000"
     CDS             1553..2035
                     /locus_tag="PLG24_03000"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HMZ23883.1"
                     /translation="MDKILTSEALIRFSDCDPMGHLNNMRYLDYFLNAREDQLKQYAG
                     FDIYQHIKKTGQIWVMFQNKITYLKPVFYNHTVLISSRIIGMSKKSIKVEMQMKDAGT
                     GDLKCVLWMIAVYFDTLRNQSAEHQPEIEAMLQQYLSPVVQQTFDERVKYLQEKYRTN
                     "
     gene            2057..3049
                     /locus_tag="PLG24_03005"
     CDS             2057..3049
                     /locus_tag="PLG24_03005"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HMZ23884.1"
                     /translation="MRLSRIFFLGTFMVLGMVGGAQDKIDYSRQENWASLPEKDDNAD
                     QLPGKNGVDRQEDYPVDVFFLYPTSFTGQVKNNQWNAPIDDVAINAETANKSIKFQAS
                     IFNKVGKIYAPLYRQANIAVFFMDDKAFAAKVLDFAYQDVRDAFRFYIEHYNHNRPFI
                     IASHSQGTAHAKRLIREEIEGTALAERMVVAYLVGMPVDTGYLSFPPCTSPEEIGCIC
                     SWRTFIHDYTPEYIAKENPAIVTNPITWINDSEYSDRKKHKGSVLLDFDKGVQPNIVS
                     ARRSGNILWIKKPRIKGRLFLRIKNYHIGDFNLFYQDVQENAILRRDAFLARLR"
     gene            complement(3042..3626)
                     /locus_tag="PLG24_03010"
     CDS             complement(3042..3626)
                     /locus_tag="PLG24_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMZ23885.1"
                     /translation="MLQQIINLDQEVLLWIHRHLHGTVSNAIIPFLRKPLFWLPLYLI
                     LIGYFIRLWRVKAYIPILCLGVCVGVADFLSVHLFKNYFKRLRPCHSLDDVPGFDLLV
                     NCGGQFGFISSHATNHMAMAVFLFLLTRKRWGRWSYLWIFWAILIGFAQVFVGVHYPL
                     DVLVGFLFGGVIGLAFYQLMNNFFPAFTKKNEVT"
     gene            3966..4913
                     /gene="fmt"
                     /locus_tag="PLG24_03015"
     CDS             3966..4913
                     /gene="fmt"
                     /locus_tag="PLG24_03015"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HMZ23886.1"
                     /translation="MKIVFFGTTDFAVASLDALVQAGHDVAAVVTNIDKLGGRGRKEV
                     IESAVSKYASQKGLNVLKPKTLKGPKFLAKLKSLEADLFVVIAFRMLPEQVWAMPPKG
                     TVNVHGSLLPAYRGAAPINWAIIRGEKLTGVSIFFLDKSIDTGNVILRKTVQIRDDDN
                     MGSLYEKMKPLGGEALLEALAMISSGNYVPEKQDESKVSAAPKLDKTNTHINFSMPVL
                     DIINLCRGLDPSPGAWFTWDGITIKLWKARQSDDILASGEWKSDFKTTLTIGCGIGSI
                     SILELQPEGKKRMSIKEYLNGAGNKLRNMDVNGQKYIKN"
     gene            complement(5477..5710)
                     /locus_tag="PLG24_03020"
     CDS             complement(5477..5710)
                     /locus_tag="PLG24_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23887.1"
                     /translation="MKKRYLPLTALLVIAAYIFTMYNGLNDSSYQAYIAQKEYENSLV
                     ADNDTQTEGKSGMVIRFINGVIDAVTMIDKVVK"
     gene            complement(5751..8372)
                     /locus_tag="PLG24_03025"
     CDS             complement(5751..8372)
                     /locus_tag="PLG24_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HMZ23888.1"
                     /translation="MIKHQNYNILQLGQGKYTRVFCMELDSGKKVAIKVLKPAYEKNK
                     FFQDQLIREAKLGMQLRHPSVRKVLDLIHDDVEGTYQVMMEYVDGYNMKDYIENFGTI
                     PEKQVVEWLMNILPGLNQAHRAGIVHGLLKPGNFIINPMGKLKICDFKGGYFESELFP
                     EDVDTQDILYLSPEQIQHMGSVTGQSDIYSLGVTLYTLLSGRLPYDADFADVNEVRLR
                     ILNQPLPFIDHVSVQLNSIIQAATSKNPMERYSGAERLMQELEGDLSAKFSTVFTKAQ
                     KIFDDEQTILTGEENHPDTSSDPVVESLVVGGDKPKADDETIKEEIIGAVSDSIDSDH
                     LPNDVSTQVDSPDVQIIGTDGDTTAPVSSDRELSGVTNADTDVSSKDLDDRVNAIWAR
                     IGVSGDNTEPVVDKVPVIQLKDYEERETAKAKEKKEKEKKKDDQIKSGTVKKLAVRTV
                     TPDGPLAEPPQVVRKAMENPVTEDNTIESSAATATAITPVKIKLHEEKATVEKRLEEP
                     TGQKLVAGQTSGYIKIFLGTILALGIVAVGAYVLYNRSAGDSVKIVTQVEENSSPDNQ
                     ANLPVATTPEVSQAEQAVVDSVIQADQSQSLEELEKAKLEKLKEMAALKKKQEEEAKK
                     KEEAKNVDKANIELLGPYFNEIAPFKQNDLIGFIDKDGKVIKKPVYEEILSFSNGLAP
                     VRLNSKWGFVNSLGKEVIKPKYNEIFGFSKGLAGVKKDNKWGFINTSGAVVVPFQYDV
                     VTDFAGGMAGVRKNGKWGFVSKNGMEAITCQYDNAWSFNDGLAGVEKNNKWGFINSKG
                     DIAIPLEYGQVNNFSEGFACVEKNGKFGYINKAGKEIISCKYEAAKPFKGGTARVFDN
                     GKWVYINKSGKCVRDCN"
     gene            complement(8402..9067)
                     /locus_tag="PLG24_03030"
     CDS             complement(8402..9067)
                     /locus_tag="PLG24_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ23889.1"
                     /translation="MTDPLVVFEGADIYQSRFMILNDVGISIYPGEFCYLIGKTGSGK
                     SSFLKTIYGELPLKAGKGKVVDFDLTTLRLKQIPHLRRRMGMVFQDFNLLSDRSVEEN
                     LLYVLKATSWKDKALMRKRVDEVLTSVGLPFIGHKNIHELSGGEQQRVAIARALLNHP
                     QLIIADEPTGNLDPDTSDEIVKLLQSLCTNGTAVLFATHDYRIIEQFPGRIIKCSEGS
                     LKE"
     gene            complement(9587..9811)
                     /locus_tag="PLG24_03035"
     CDS             complement(9587..9811)
                     /locus_tag="PLG24_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23890.1"
                     /translation="MREFIPAFLLIVCGAAGLTLLQWKGDYTLTPLDGRALNTTTIAI
                     KTISGDQYEEEGGIRILDISDISITADSLQ"
     gene            10426..>11420
                     /locus_tag="PLG24_03040"
     CDS             10426..>11420
                     /locus_tag="PLG24_03040"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ23891.1"
                     /translation="MTVSKIFSQSYAEGCDGIRYGYQIFDKTTKTTVRYATSTPEKKD
                     LFMDIYQPVNDQVSKRPLIIFAHGGSFIFGDKSDMESFCTFWAQRGFVTASVQYRLFA
                     GIPTPATMFDAAVKAVSDMKGAYRFFKTDAANLNIYQIDTTKIFIGGLSAGAITALHA
                     AYMDEGDPIPPDALTTINANGGFHGNTGDAENLSHNDRDIFGVINMSGAVFSKGLLDE
                     DEPFLMSFQGDKDEVVPIDSNSVFFGLAYLYGSRTIHKEADAIGLPNSLTVASGGGHT
                     DIYLNPAFAADLDNFIDNSLALFYPKLCGTNLSTKYADRPSLNITTYPNPATSRL"
BASE COUNT         3456 a         2555 c         2100 g         3309 t
ORIGIN      
        1 ccctatatat ttgaatatta aaaagtatgt gtgataataa cgaaaaaaaa acttatacca
       61 ggctacttat tgtccatcgt gcaaaaactc atccctccca aagaccatat cagtgaatcg
      121 gtcataatgt tcactcaaat aaaaggcacc tctctgactg tgaattaaat ttgaacagaa
      181 cagacaatcg ctaagcagta cagagctaat tacgtctgtt cttgtaagca tccagtgtat
      241 tcctgatgag gctcacaata gtcatcaggc ctattcctcc cggcacaggt gtaatccagg
      301 ccgccttatc ctttacctgc tcgaaatcca catcgcccac caatcggctt cccgacttag
      361 ccattggatc cggtatccgg ttgataccaa catcaaagat ggttgatcct tcctttacca
      421 tgtcttcttt gacataatgc ggtatgccaa tagccgcaac aataacatct gccgcaagca
      481 gttcttcctt gatattttta cttcttgaat gcagcaacgt cacggttgca tttccataag
      541 gagccttgcg ggacagtaac agggaaatcg gcgtccccac gatatcgctc ctaccgagaa
      601 ctgccacctt ttttccggaa agatccagtc cgagatactc gataagcatg actatgccat
      661 aaggcgttgc cggaaggaaa gcaggcagac ttaaagccat cttgccgaaa ttcatcggat
      721 ggaagccatc cacatcctta ttgggatcta tttccagtaa aagcgcctga ggatcaaggt
      781 ggtcaggcaa tggcaactgg agtataaatc catccacctc atcgtcagca ttaagtcggc
      841 ggatgacctc aaccaactct tcctgcgtaa tcccggtttc gaaatgcaac agggtgctct
      901 tgatacccaa ttcttcacaa gttttcacct tattacccac atatgcttta ctggcgggat
      961 tgtcccccac cataatcgct gccagatggg gtggcctggc cccggatgcc actatgtcag
     1021 ccacttcctt acctatgccg gccttcagtt ccttactgat cgatctaaca ttcaactcag
     1081 ccattttcaa ttcaatttat tccaatcaca atgataataa tattttatgc gaaaacagaa
     1141 ataaatgcac ctgaaatagt accaatactt tgttttaaaa caatattaaa aaacaagctt
     1201 ataaagtacc tcatataatt accgatacgt tgcattcaaa atcaatgccc ttcgcaatga
     1261 tttcctttga atcagacggg acagccggaa tcaaccgtca aaatctatgc aaaaacaggc
     1321 aatttcctgt tgattggaga atatcgaagc agtgaaatcc tttggagaaa gatgacaaac
     1381 aggctttgat ctaaaaatct gtagaaattt ttaccacaaa aaccaaccga aaccaagcga
     1441 ttatcctaaa ataaaggcat cgctcttgct catcctgaag aaaatctcat tttcacattt
     1501 gaagcctaaa caatggtact atttacttgt ttgtatatcc aaaattgata atatggataa
     1561 aattctgact tcagaggctt tgatccgttt ttcagactgt gacccaatgg ggcatctcaa
     1621 caatatgaga tatctcgatt acttcctcaa tgcgcgggaa gatcaattaa agcaatatgc
     1681 cggtttcgac atttatcaac atatcaaaaa aaccggccaa atctgggtca tgttccagaa
     1741 taagatcact tatctgaaac ctgtattcta caatcataca gtccttatca gtagcaggat
     1801 aatcggcatg agtaaaaaat caatcaaggt ggaaatgcag atgaaggatg caggtaccgg
     1861 cgatcttaaa tgcgtgttgt ggatgattgc tgtctatttc gataccttgc gtaatcaaag
     1921 tgccgaacat cagcctgaaa ttgaggccat gctacaacag tatttgtcac ctgtggtgca
     1981 acagactttt gacgaaagag taaagtatct tcaggaaaaa tatcggacaa attaaattct
     2041 ataaaaggct aattttatga gattgagcag aatattcttt ttgggcactt tcatggtgtt
     2101 ggggatggtt ggcggagcac aagacaaaat cgactattcc agacaggaaa actgggcatc
     2161 acttcctgaa aaagacgata atgctgatca gttacccggc aaaaacggtg tggatagaca
     2221 ggaggattat ccggtagatg tgtttttcct atatccaaca tcctttacag gccaggtaaa
     2281 gaacaatcaa tggaatgcac cgatagatga tgtcgccatc aatgcggaga ccgcaaacaa
     2341 atccattaaa tttcaagcca gtatcttcaa taaagtaggg aagatctacg cacccctata
     2401 tcgtcaggct aatatcgctg tgtttttcat ggatgacaaa gcttttgccg ccaaagtcct
     2461 tgattttgcc tatcaggatg tcagagacgc cttccggttc tatatcgagc attataatca
     2521 caaccgacca tttatcattg cttcacattc acagggaact gcccatgcta aacgattaat
     2581 cagggaagaa attgaaggta cggcacttgc ggaaaggatg gtggtggctt atctggttgg
     2641 aatgccggtt gatacaggct atcttagttt ccctccatgt acctccccgg aagagattgg
     2701 gtgcatctgt agctggcgga cattcatcca tgattatacg ccggaatata tagcaaaaga
     2761 aaatccggca atagtcacta atccgatcac atggataaat gattccgaat attctgaccg
     2821 aaaaaaacac aaaggttcag tgctcttaga ttttgacaaa ggagtgcaac ctaatatcgt
     2881 ttcagccaga cggtccggca atatactctg gattaaaaaa cccagaataa aaggaaggtt
     2941 attccttcgc atcaaaaatt atcacatagg tgacttcaac ctgttttatc aggatgtaca
     3001 agaaaatgca atcctgagac gtgacgcttt tctggcaaga ctcaggtgac ctcatttttt
     3061 ttggtaaacg ccggaaaaaa gttattcatc aactggtaaa atgccaatcc gatcacgcct
     3121 ccgaacagga agccgaccaa aacatccagc ggatagtgta ctccaacaaa tacctgtgca
     3181 aaccctatca aaattgccca aaaaatccac agataactcc atcgccccca ccttttcctt
     3241 gtcaataaaa acaggaatac tgccatagcc atatgattgg tagcatgact ggaaataaat
     3301 ccaaactggc cgccacagtt gaccaacaaa tcaaagccgg gcacatcatc caacgaatgg
     3361 cagggccgca agcgcttaaa ataattcttg aaaaggtgaa cactcagaaa atcagcaaca
     3421 cccacacaga cacccagaca cagaatagga atatatgctt tgacccgcca tagccttatg
     3481 aaataaccaa ttaatatcag gtataaggga agccaaaaca aaggtttccg caaaaaagga
     3541 atgattgcat tagacacagt accatgcaaa tgtcggtgga tccataatag aacctcctgg
     3601 tccaggttaa taatttgttg tagcatacct gtattgcttg acaaacagcc gctaaagata
     3661 aattttttat tgggtgtaaa gactataaat atataattga atttccgcca atattgtcaa
     3721 agctccgatt caaaccggtg agaatggaaa atgaatcttc attcaatcaa ccacctcttt
     3781 gtttccaaac taccattcac atgcctagac attaatcatt aaaaatatcc cgtagtttaa
     3841 aagccaatgt tagtcacttc aaagactctt tgatagtaca atatataatg agaaaaccta
     3901 tatcttttta attaaacgat gtaaatatca acaatgaatt aattttggcc gttaaatcaa
     3961 gcttaatgaa aatagtattc ttcggaacta cagattttgc tgttgcatca ctggatgctt
     4021 tggtacaagc aggacatgat gtagctgcag tagtcaccaa tattgacaaa ttgggtggaa
     4081 gaggccgtaa agaagttatt gaatcagcag ttagtaaata cgcctcccaa aaaggcctca
     4141 acgtattaaa acctaagact ttgaaagggc ctaagttctt agccaaacta aagtccctag
     4201 aggcggatct tttcgtcgta atcgcattcc gcatgttacc tgaacaagtg tgggccatgc
     4261 caccgaaggg cacggtcaat gtgcacggtt ccttattacc tgcttaccgc ggagcagcac
     4321 ccatcaactg ggctatcatc aggggagaga aattgaccgg agtatcaata ttttttctcg
     4381 ataaatccat cgataccgga aatgttatac ttagaaagac agtgcaaatc agagatgatg
     4441 acaatatggg ttccctatat gaaaaaatga aaccgcttgg tggagaagcc ttactggaag
     4501 ctttagccat gatttcttcg gggaattatg tgcccgaaaa acaggatgag tcaaaggtca
     4561 gtgcagcccc caaactggac aaaaccaata cacacatcaa tttttcaatg ccggtattgg
     4621 atatcatcaa tctgtgtcgt ggcctcgatc cctccccagg tgcctggttt acctgggatg
     4681 gcataactat caaactttgg aaagcaagac agagcgatga tatccttgca tcaggagaat
     4741 ggaaaagtga cttcaaaact accttgacca taggctgcgg tattgggtca ataagtattt
     4801 tagaactgca acctgaaggc aaaaaacgaa tgtcaatcaa ggaatatttg aatggcgcgg
     4861 gaaataagct aaggaatatg gatgtcaatg gccaaaagta tatcaaaaat tgaaccgccc
     4921 catcaaagcc aatccggcac cctggttatt aaacattgat ttaggctaaa acaggacaga
     4981 attttcagac tttgggtaaa ggaaaagtaa tagcggatcc tttgcatccg gaaagtccat
     5041 tgaaaataga tggctatatt taaaaagttc tgagataagg caaactcata ctttgatcag
     5101 ttggcaggga tgttccaaca gtttttatag taaaggtaca atcaaacagc ccttagaagc
     5161 aatgggatga atgattcaac aacaacatta ttccattttt gctgccttcc aacccaattt
     5221 attttccgga atgtaaaaag tatgatcaag aatatgaatt caaatagggc gatgtacatt
     5281 aatccggcat tcccgcaaac caaaggatcg gatatcaaac gatttcataa aaatcaagct
     5341 tcaacgctaa tatctctaag taagaacaaa gacaggttcg gctaacctta caacttttac
     5401 ataacccgtc ttacttcaca acaaataaaa ataatcggaa aaatattctg taccgaacta
     5461 aatggttttg tgatgattat ttgaccactt tatcaatcat tgtcacagca tcaatcactc
     5521 cgttaataaa acgaattacc ataccggatt tgccttcagt ctgcgtatca ttgtcagcga
     5581 caagactgtt ttcatattct ttctgggcaa tataagcctg atagctactg tcattcaacc
     5641 cgttgtacat tgtgaagatg tacgctgcga tgaccaaaag tgcagtaaga ggtaaataac
     5701 gctttttcat atcagcttaa catttcttaa ataacgtata aagatacaat ttagttacaa
     5761 tccctaacgc attttcccga tttatttatg tacacccact tcccattgtc aaaaaccctt
     5821 gccgttccac ctttaaacgg cttggctgct tcgtatttgc aggagatgat ttccttgccg
     5881 gctttgttga tatatccgaa cttaccattt ttctcgacac aggcaaagcc ctccgaaaag
     5941 ttattgacct gtccatattc caaaggtatg gcaatatcgc ccttactatt gatgaatccc
     6001 cacttgttgt ttttctcaac acctgccagt ccatcattga aggaccatgc attgtcatac
     6061 tggcaagtga ttgcttccat gccatttttg gatacaaagc cccattttcc gtttttccta
     6121 acgccggcca tgcccccagc aaagtcagtc accacgtcat actggaaagg tacaactacg
     6181 gcacctgaag tattgataaa accccattta ttgtctttct ttacgccggc caatcccttg
     6241 gaaaagccaa atatttcatt atacttgggc ttgataacct cttttcccag tgaattgaca
     6301 aagccccact tactatttag tctaacaggt gccaatccat tgctgaagct gagaatttct
     6361 tcatataccg gcttcttgat taccttaccg tctttatcaa tgaagccaat cagatcgttt
     6421 tgtttgaatg gtgcaatttc attgaagtat gggcccagca attcaatatt ggccttatcg
     6481 acatttttcg cctcttcttt cttcttagct tcttcttcct gctttttctt caaagccgcc
     6541 atttccttca gtttttcaag ctttgccttt tcaagttctt caagactctg gctttgatca
     6601 gcctgtatga ctgaatcgac aacggcctgc tctgcttgac tcacttccgg tgtagttgca
     6661 acaggtaaat tggcttgatt atcagggcta ctgttttctt caacctgtgt aactatctta
     6721 accgaatctc cggcgctccg gttatagagt acatacgcgc cgactgctac aatacccaat
     6781 gctaaaatag tccccaaaaa aatctttatg taaccggatg tttgccctgc aaccaatttt
     6841 tgcccggttg gttcctctaa gcgcttctcc acagtcgctt tctcttcatg aagtttaatc
     6901 ttcaccggcg ttattgctgt tgcggtggca gcagaggatt caatggtatt atcttcagtc
     6961 acgggattct ccatcgcctt tctgaccact tggggaggct cagctaaggg gccatccggc
     7021 gttactgttc ttactgccag tttcttgaca gtgccggatt tgatttgatc atccttcttt
     7081 ttttcctttt cctttttctc tttagctttg gccgtttccc gctcctcata gtctttgagt
     7141 tgaatcacag gcactttgtc taccaccggc tccgtattat cgcctgaaac tccaattctt
     7201 gcccagattg cattcacccg gtcatcaaga tctttgctcg acacatccgt gtctgcgttg
     7261 gtgacgccac tcaattcacg gtcagaggaa acgggtgccg ttgtatctcc gtctgtacca
     7321 ataatttgta catcaggtga atcgacctgg gttgacacat cgtttggcag atggtcagaa
     7381 tctatactat ccgaaaccgc cccaatgatt tcttctttaa tagtttcgtc atctgcctta
     7441 ggcttgtcac cacccactac taaagattct actaccggat cagatgatgt atccggatga
     7501 ttctcctccc cggtgagtat tgtctgttca tcatcaaaaa tcttttgggc cttggtaaaa
     7561 acagttgaaa actttgctga tagatcacct tccaattctt gcataaggcg ttcagcaccg
     7621 gaataccttt ccattggatt cttggaggtt gctgcctgaa taatgctgtt caactgaaca
     7681 gaaacatgat caataaatgg aagcggctga ttaagaatcc tcagtctgac ttcattgaca
     7741 tcagcgaaat cagcatcata aggcaatctg cctgaaagca gcgtatataa ggtgacccca
     7801 agagagtata tatctgattg accggttact gaacccatgt gttgaatctg ctcaggactc
     7861 aagtacagta tgtcctgagt gtccacatct tccgggaaca actcactttc aaaataacca
     7921 cccttgaaat cacaaatctt aagtttaccc atcgggttga tgatgaagtt acccggtttt
     7981 aataatccat gtacgattcc cgctctatgt gcctgattca atccgggcaa aatattcatc
     8041 aaccattcaa ctacctgttt ttcggggata gtaccaaaat tttcgatata gtctttcatg
     8101 ttgtacccat cgacatattc catcatgacc tgataggtac cctctacatc atcatggatc
     8161 agatccaaaa ccttgcggac agaaggatgc cgcagctgca tgcccagctt ggcctccctg
     8221 ataagttgat cctggaaaaa tttatttttc tcataggcag gcttcaaaac tttgatagcc
     8281 actttcttgc ctgaatcgag ctccatacaa aagactcggg tatattttcc ctgtcctaac
     8341 tgtaagatat tgtagttttg atgtttgatc acgatattta cattaaaggg taatccatta
     8401 tttattcttt caacgaacct tcactgcatt tgatgattct tccggggaat tgttcaatga
     8461 tacggtagtc atgagtcgca aataaaacgg ctgtaccatt cgtgcaaagg ctctgcagta
     8521 actttacaat ttcatctgaa gtgtccggat caaggttgcc ggttggctca tcggctataa
     8581 tcaactgtgg gtgattcaac aaggcgcgtg caattgccac cctttgttgt tctcctcccg
     8641 ataattcgtg aatattttta tgccctataa atggtagtcc aaccgaggta agtacttcat
     8701 caacccgttt tcgcattagg gctttgtctt tccaggaagt ggccttcaat acatatagaa
     8761 gattctcttc gacgcttctg tcactcagga ggttgaaatc ctgaaacacc atgcccatac
     8821 gtcttcgaag atgcgggatt tgcttgagac gcaaagtagt caggtcaaaa tcaaccacct
     8881 tccccttgcc tgccttcagc ggtaactcac cataaattgt cttgagaaaa cttgactttc
     8941 ctgatccggt cttgcctatg agataacaaa attcacccgg ataaatactt atcccaacgt
     9001 catttagtat cataaaccgg ctctgatata tatcggcacc ctcaaatacc accaatggat
     9061 cagtcataat tattttgatt acctagttct tcttaaataa aaaacaaaaa taaacaaatt
     9121 aattattatc aaatatgttt ttgcaagttt tttttatata tacctaattg ttgttcagtg
     9181 caattataaa ttcaataatt tttaaagtac cggattatag cttgttcggt agtccacgga
     9241 acaggaaata ccggctcttt aacccaactt caaaaaaata ctatgaataa acaagcaatt
     9301 cctcattact tcaaaataca ccgtaatcta tattaatcag taattatatt cgtctataca
     9361 tgcactaatt atataattgt caattattta cctataataa aaaaaagaac attaaggaaa
     9421 ttgctatgat tccggcaaaa cagatcatga aggaaatcct atgaaaagtt tgaatcgttg
     9481 cggaaccaat attcgcttta taccttgtct accgttttat ttccccggat ccaattcatc
     9541 acgacacctt tgtcgccctg tgccacaaat gagctgaggg acaaaatcac tgcagggaat
     9601 ccgccgttat cgaaatatca ctgatatcca ggattcggat gcctccttct tcctcatatt
     9661 gatcaccgga aatagtttta atggcgatcg tcgttgtatt gagtgcccgc ccatccaacg
     9721 gggttaatgt gtaatctcct ttccattgaa gcagggtcaa cccggccgca ccacaaacaa
     9781 ttaataaaaa tgccggaata aattctctca taacttcgat actatttgtt ttatactgtt
     9841 ttatatatta aataattata atacgtatat taaattgtaa tacaaatata tatgcattat
     9901 aattattttt tatatctaag taatgtaatt ttaagaaccc atgattccgt tcaataatat
     9961 tatataaata aatattttta tttatttatt tgactaatta ttatacatta aatatattta
    10021 ttatattaat tataaaacta tattaaatca tatccttggc tgaccatcca ccgaaaacga
    10081 gcattgagtt gactaccaaa aactgtacat gtttgacgaa caattatatg attgacagca
    10141 taatatgcat taacaatact tttatataaa tatcgtgcgt gttgattacc tctccctttc
    10201 agttcaaaag gtatatcctt gaatcttcct cctgccaaat ccctgccaaa ttggactacc
    10261 aatcctccta ccggcaaaga ataacaccaa ataccttagg atggagctga gaacttactc
    10321 aaaagtcaat tccttcttga atctttgatt ccaaaattta ttataacttt atccgtacaa
    10381 ttcaatttta tgagaaagtt tttattctgt ttcatttttt ttaccatgac cgtgtccaaa
    10441 atcttcagcc agtcgtatgc agaaggctgt gatggaatca ggtatggcta tcagatattt
    10501 gacaaaacca cgaaaacgac tgtcaggtac gccaccagca cacctgaaaa aaaggatttg
    10561 tttatggata tctatcaacc tgtcaatgac caggtgtcaa agcgaccact gatcattttt
    10621 gcgcatggag gtagctttat cttcggagat aagtctgaca tggagtcatt ctgcactttt
    10681 tgggcacaac gaggattcgt cacagcctcg gtacaatatc gtcttttcgc gggaattcct
    10741 actcctgcaa ctatgtttga tgctgcggta aaggctgttt ccgacatgaa aggtgcttat
    10801 cggtttttca aaactgacgc tgccaacctg aatatttatc aaatcgatac aacgaaaatc
    10861 tttatcggcg gtttgtcagc cggcgccatt accgcattgc atgcggctta tatggatgaa
    10921 ggagacccca tacctccgga tgccctgaca accattaatg caaatggggg atttcatggt
    10981 aatacaggag atgctgaaaa cctaagtcat aatgatcgcg atatctttgg tgtgatcaat
    11041 atgtcggggg ctgtcttttc aaaaggtctt ctggatgaag atgagccgtt cctcatgagt
    11101 ttccagggag ataaggatga ggtagtgccc atagacagca acagtgtatt tttcgggttg
    11161 gcatatttat acggttcaag aacgattcac aaggaagccg atgcgattgg tctgccgaat
    11221 tcactgacag tggcttcagg aggcgggcac accgacatct accttaaccc tgcttttgcg
    11281 gctgacctcg acaattttat cgacaacagc ctggccttat tctatccgaa attatgtggc
    11341 actaacttga gtaccaaata tgccgataga ccatccctga atattacaac ctaccccaat
    11401 ccggcaacga gccgtctcta
//
LOCUS       DAOQQN010000049        11338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2033_length_11338_cov_5.344057, whole genome shotgun sequence.
ACCESSION   DAOQQN010000049 DAOQQN010000000
VERSION     DAOQQN010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11338
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2033_length_11338_cov_5.344057"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1577)
                     /locus_tag="PLG24_03045"
     CDS             complement(<1..1577)
                     /locus_tag="PLG24_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HMZ23892.1"
                     /translation="MEAINFKVNEVPLSGTNLIEASAGTGKTFSIGILVLRTIVEKNI
                     PIDNQLIVTFTNYAVAELQDRIRKFLSQAIEIASASEQELVLIADRFEQPIVEICERF
                     EDKTELITRLRQSQLLLDEASIFTIHGFCQRMLNEFAFESGQNFQTELQADVSYLIER
                     VTNEFWRNEITTLDKDLFESKDLKKVRAYFKEILKQGLNNTQFDVNVSSPIKDNGKIS
                     DIIECYKGYLKELETEWERLECFIAEDNTRLKVDVESNSNTRKKYSDLLGQPRLMLQV
                     TFDYFKANKAAGYFDKFSSPYWNDVKSLVERENNLNNEKNKVIVAILYANRDYYLDVL
                     NQSINDSGVLTFDHLILNLHRSIVAGNNKKLKELIRQKYRVAFIDEFQDTDVIQFQLF
                     EKLFIDDQYDDDTTLFLIGDPKQSIYSFRDADVESYLHAKSKVDHIYTMNTNFRSSTR
                     MIEAANEFFKASGDNVFGYEAEKETGIYIGYTEIQANKHNKKFLRRQEEVEETLLFCN
                     ANDAATSQTQLVETVKYL"
     gene            complement(1591..4755)
                     /locus_tag="PLG24_03050"
     CDS             complement(1591..4755)
                     /locus_tag="PLG24_03050"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HMZ23893.1"
                     /translation="MGIHIPISNDITKLADAMIEDIGLYRFDVFQPVYIVTQTKGMNS
                     WLKYRMSEQHGVAANLMFLTPEEIVYEVYKILGGSYKDKIDRKDMDWLIYAALSDEAF
                     KEKFPERASYFYNEEGRDKMKQWELAAKMGDLFDQYQIYRSEVIAQWKVNELESVEEK
                     SRWQAYLWKKIDSIAGDKIIDFNAIKVDILEKLKTVQNQERLVRNIPVLHLFGLSIIT
                     KYHLEIIHAMSEHIDIRWYANNPAPDYYWFEDEPQKVLDKWKAKNRRIEHITVGHPLL
                     TDWGKVIQSIFRLISGHEEFINAITDLPLKQNNGTTLLASLQQSIQHNSVDRPDFDTG
                     LFDDDTISITSNYSKRREVETLYNYILDTIATKRHGDILDREIIVMATDINAYAPYIR
                     AVMDNAPYRFKYNIADEALANVNSPVAALISLLEFDGKLFGAEEVLGLLTHKYIREKF
                     RINDLELIRNTIVKANARFGIENDYSTPVNDTFIVSWRYALEKIFYGICLPGEIRIDN
                     EHPFYTVETTDTVAGIQEISAFLAFFEALKTHVHERKNPKPLREWRKFIEQVLEDMFA
                     IPDDEKNEDYVKKIEKLIRFENLDDGLKDEEIPYDVFSKRFLTGLKVETTSTRFMSRG
                     ITFCSPLPFRSLPFKLVCMLGLNHGEYPRLERRSDFDLIASNRKLGDRNLKENDKHLF
                     LESILSAEKALYLSYIGRSIKDNEILPPSILIDELLDYLQMGNVEVDVRKKLVSEHPF
                     YSFSTKYNKDSGNLLPNYFIQLAKEWPIPYNDNREVPEIKEYDINTVWKFFEKNIQYY
                     YNNVLGVYFEREDGDIKDTENFELDSLEEWTVKESLLDSRLKGVEQALEEKLYLSGQL
                     PLKNVGLGLVDEIDEVISPLVDDVKNDFPDNFDPKIVSIEITYDDVRYFGEIKNVIDK
                     KIISFHHGSSGGEAKRRARLAFQYLFAVVCNVDITGGALYTLNGKHTLKQLGVDEARA
                     RLGNILTFFHENKERLIGYSAEQYFQSLKEDDSFDKIKNFFFIDRNYRDDYYRHVMLN
                     HDINIHRVWDMHVILQEILLDNAY"
     gene            complement(4760..6505)
                     /gene="recD"
                     /locus_tag="PLG24_03055"
     CDS             complement(4760..6505)
                     /gene="recD"
                     /locus_tag="PLG24_03055"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HMZ23894.1"
                     /translation="MDISSINSADAFAGFFAKDDLFPWLRTLIEQMVLGNICIDIDAF
                     SESQYHNLSILSDHILVGPPQENKPFVIHNGRLYINRYFQYEKSLIEKIRQLCELVAA
                     DRDFDFLHDNKDFIVRHLFPPSGYVKGADWQLVACLTAFLSRFTIISGGPGTGKTTTV
                     AKLLYLLLKQTPDAKIGVCAPTGKASAHLDESFARAGDKLAGSEFNDELVQKIADLKA
                     LTLHSMLGYIHNSIHFKHHEGNPLDFDILIVDECSMIDITMFFKLFKAIDPGRTRVVL
                     LGDKNQLASVEAGSIFRDLCNDTAPMNIFSKVRTDFINGFIEEPLDCMSGEVTHPLFE
                     RMIELQTSFRFSDDEGIGKFSKAVLNNDQVVIKEFLENKDDRVKFLCPNDVESALKSA
                     AQRLLQVNGGYVGQTGVGEALNRLVKSIVLCAVKEGKTGVLAVNRMIASEVSHNNERF
                     YPFLPVMVASNQHSEGIYNGDMGLILEVKGEVNAYFPKGNGENLKLNPAQIREWEPAY
                     AMTIHKSQGSEFDEVLIILPENRENILLTRELLYTAITRAKEKVTIVGFSEIIRAIAE
                     QSVKRVSGIKDHFKS"
     gene            6659..7615
                     /locus_tag="PLG24_03060"
     CDS             6659..7615
                     /locus_tag="PLG24_03060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HMZ23895.1"
                     /translation="MPKSSVLHITHDSFVKLVLSNPAAGQDFLNYFLPESLKGNLDIE
                     KCVPSELSYLTESMRNRHTDLIFDVPIKGSGEGIRIAILIEHKSYKDEAVIFQLLEYL
                     ALGYRKQSREKKGFRLIIPLLYYHGKQEWSPAGLSSYFIDIPGDLKPYLPEFSRIYID
                     LKNYSIDDIERLNNSLLRTALYIQLLRLQNMENKTILSRAFTTFEYGQYRNYFSAIFV
                     YAIKYLDLEKEEFEILVDEFPEEVKPEAMTLANRLKREGMEEGIEIGKKQGIEKGIEK
                     GIELSQVSFVFKSYENGLDALTIAKIVGITEDRVREILNIER"
     gene            7803..8519
                     /locus_tag="PLG24_03065"
     CDS             7803..8519
                     /locus_tag="PLG24_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ23896.1"
                     /translation="MKFPIQVEQKNCLNCAFKGHSLFSILSDDSLDHLNAHKSCTIFK
                     KGQYLFTQHSRPMGLFCVYSGKIKLSTAGPDGKEQIIRLAKDGDVIGYRAIITDENYR
                     LSAIALEESSVCLIEKEFFLDLAMSEPRLNRQIFKLISDDLKRAEEQIVSLSQKSVRE
                     RTAEALLFLKATYGFEDDNQTLNIRLSREELADYVGTTTESVIRVLSDFKTSGLIDIA
                     ARKIIIIDLGLLIKTANMIP"
     gene            8940..9431
                     /locus_tag="PLG24_03070"
     CDS             8940..9431
                     /locus_tag="PLG24_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23897.1"
                     /translation="MNISKCVILTLGIMSLLSCKNDSHTTEHGSNESSHAAHVSTIDS
                     TQPPMFNEIMAVHDEIMPWMNNIETLKQDIRMKMDSIDAFGGSDANRKQFRHAMAELN
                     RADNAMTDWMSSFKENYDTIQIEKNKMVFLESERAKIEGVKNLMTESIKVAQLTLKNT
                     PIK"
     gene            9450..10049
                     /locus_tag="PLG24_03075"
     CDS             9450..10049
                     /locus_tag="PLG24_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HMZ23898.1"
                     /translation="MTFRFFIFSLLLLVLNGCNTKPEPLPFLGIKVHEDDKPQIPEYH
                     FIDQLNRPVTPETFKGKIYMTDFFFTSCPTICPIVTGQMLRIYKEFEKDDRVAFLSHS
                     IDTRHDSVPKLKAYADKLHINDDKWRFITGKKEDIFNISKNYLSMVLEDPNTPGGFDH
                     SGYIILVDKDRHVRGFCNGTDPKDVDKFMGKVKQLLDTE"
     gene            10046..10429
                     /locus_tag="PLG24_03080"
     CDS             10046..10429
                     /locus_tag="PLG24_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMZ23899.1"
                     /translation="MTRWLIVLLSAVFVLYSGCTTYSQGKRIYESKCANCHGIKGEGL
                     AALYPALDKSIIITHHKSRIPCIIELGRSSRDSLGNLTSEMPPVKGLTVAEITNLINY
                     LLTLNPNGQETVTIDSVKVWSEACN"
     gene            10584..>11338
                     /locus_tag="PLG24_03085"
     CDS             10584..>11338
                     /locus_tag="PLG24_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23900.1"
                     /translation="MKYDAVGKIFVENCEATKGTIVFIFDNQQVERRFEVKFTGDATP
                     GTDFQVDGLSDTLVVGSGIQTIELPFTVNVDNVAEGREDLTITLVSIPEGQEIASYST
                     SIYDEFSVSINDGVQVESCKGQKLLLTASEAGTFLWTPSENFTDATKQTVEYVSNESS
                     TVHLTAGLGSCTANATINVSIINTDVLLTADKDTICGPSLVTYTATAATQGGVFTWSP
                     AELFPNQNSATQSVLIKANTQVKVSYSKGDCTSS"
BASE COUNT         3482 a         2376 c         2025 g         3455 t
ORIGIN      
        1 aggtatttga ccgtctcaac taattgagtt tgacttgtag cagcatcatt agcattgcag
       61 aaaaggaggg tttcctcaac ttcctcttgc cttctcaaaa acttcttatt atgcttattt
      121 gcctgaatct ctgtataacc aatataaata cccgtctcct tttcagcctc ataaccaaac
      181 acattatcac cagaggcttt gaagaattca ttggcagcct ctatcatcct tgttgaagac
      241 cgaaagttgg tattcatcgt ataaatatga tccacttttg acttggcatg aaggtagctt
      301 tcaacatcag catctctgaa tgaataaata ctttgttttg gatctccaat cagaaataag
      361 gtagtatcat catcatactg atcatcgata aacaacttct caaatagttg gaattggatg
      421 acatcggtat cctgaaattc atcgataaag gcaacacgat atttttgcct tatcagttct
      481 ttcaattttt tattattccc ggcaacaatc gaacgatgaa gattaaggat tagatgatcg
      541 aaggtgagga ccccggaatc gttgatagat tgatttaaaa catccagata ataatcccta
      601 ttagcatata ggattgctac tatgacttta tttttttcat tgttcagatt attctctcgt
      661 tcgaccaatg attttacatc attccagtag ggtgatgaaa atttatcaaa atatccagct
      721 gctttattcg ctttgaagta atcaaaggtc acttgcagca tcaacctggg ctgacccaat
      781 aagtctgaat atttcttccg ggtatttgaa ttgctttcaa catccacttt tagccgtgta
      841 ttatcctcag ctatgaagca ctcaagccgc tcccactctg tttccaattc ctttaaatat
      901 cccttgtaac attcaataat atcagaaatt ttcccattat ccttaattgg gctggaaaca
      961 tttacgtcaa actgcgtatt attaagacct tgctttaaaa tttccttgaa ataggcgcga
     1021 actttcttta aatctttaga ttcaaagagg tctttatcca aagtggtgat ttcatttcgc
     1081 cagaattcat tcgttacccg ttcaatcagg taagaaacat cggcctgtaa ttcagtttga
     1141 aaattctgac ctgattcaaa agcaaattca ttcaacattc gctggcaaaa accatggatt
     1201 gtaaaaatac ttgcttcatc taaaagaagt tgagactgcc ggagtcttgt tatcaactct
     1261 gtcttatctt cgaatctctc acatatttcc acaataggtt gctcaaatct atcagctatc
     1321 aatactaact cctgttctga agccgaagcg atttcaatag cctgagaaag aaattttcgt
     1381 atcctgtcct ggagttcagc aacagcataa ttggtgaaag taacgatcaa ttgattatcg
     1441 atcggaatat tcttctcaac aatcgttctg agcaccaaaa tcccaataga aaacgtctta
     1501 ccggtaccgg cactagcctc aatcagattg gttcctgata atggcacttc gtttacctta
     1561 aaatttatgg cttccattga attgtccatt ttaataggca ttatccaata atatttcttg
     1621 caggatgaca tgcatgtccc acacccggtg gatattgata tcatggttta gcattacatg
     1681 tctataataa tcatcacgat aatttctatc gataaagaag aaatttttaa tcttatcaaa
     1741 tgaatcatct tctttaagcg attgaaaata ttgttcagcg ctgtatccta tcagcctctc
     1801 tttgttttca tgaaaaaacg ttaagatatt acccaacctt gcccgcgcct catcaacacc
     1861 tagttgcttt aaggtatgct tgccgttgag ggtgtataga gcgccacctg taatgtcgac
     1921 attgcatact acagcaaaaa gatactggaa agccaacctg gcacgacgct ttgcctctcc
     1981 acctgaactc ccgtggtgaa aacttattat tttcttatca attacattct ttatctcacc
     2041 aaaataccgt acatcatcat aagttatttc aatacttaca attttcggat caaaattatc
     2101 aggaaaatca tttttgacat cgtcgaccaa aggactgatc acttcatcta tctcatctac
     2161 cagtcctaat cctacgtttt tcaatggcaa ttgtccggat aaatagagtt tttcctctaa
     2221 tgcctgctct actcctttca atcttgaatc aaggagtgat tccttcaccg tccattcttc
     2281 cagactatcc aattcaaaat tctcggtatc tttaatgtca ccgtcttccc tttcaaaata
     2341 cacccccagc acattgttgt aataatattg aatattcttc tcaaaaaatt tccaaactgt
     2401 attgatatca tattccttaa tctccggcac ttctctgttg tcattatatg gaatcggcca
     2461 ctccttggca agttggatga aataattcgg aagcaaattg cccgaatcct tattatattt
     2521 tgtactaaaa ctgtaaaatg ggtgctcaga aaccaatttt tttctaacat caacctccac
     2581 attccccatc tgcagataat ccagcaattc atcgataagg atagagggag gcagaatctc
     2641 attatctttt atcgaccgac cgatataact caaatataaa gctttctccg cagaaagtat
     2701 ggattcaagg aaaagatgct tatcgttttc cttaagattc ctgtcgccaa gcttcctgtt
     2761 ggatgcaatc aggtcaaagt cactgcgccg ctccaagcgg ggatattctc catgattaag
     2821 gcccaacata caaaccagct tgaatggaag acttctgaaa ggtaaagggg aacaaaaagt
     2881 gattccacgg ctcataaaac gagtcgatgt tgtttctact ttcaatccgg tcaagaaacg
     2941 cttactgaat acatcatatg gaatctcttc atctttcaac ccatcatcca gattttcaaa
     3001 tctgatcagt ttttcaatct ttttgacata atcttcgttc ttttcatcgt caggaattgc
     3061 aaacatatcc tctaatacct gctcgatgaa tttgcgccac tccctcaatg gctttggatt
     3121 ttttctctca tgtacatgag tttttaaagc ctcgaaaaac gccaggaatg ccgatatttc
     3181 ctgaattccg gccacagtat ccgttgtttc gacggtatag aatggatgtt cattatctat
     3241 tctaatctct ccgggcaaac aaatgccata gaaaatcttc tccaatgcgt aacgccagga
     3301 aacgataaag gtatcattca ctggagttga gtagtcgttt tcaataccga agcgcgcatt
     3361 agccttcacg atggtatttc gtatcaattc caaatcattt attctgaatt tttcccggat
     3421 atacttatgt gtcaatagcc caagaacttc ctctgcacca aacagcttgc cgtcaaattc
     3481 cagcagcgaa atcagggctg caacgggact gttgacatta gccaaagctt catcggcaat
     3541 attgtatttg aatctataag gtgcattatc cataacagcg cggatatagg gagcatatgc
     3601 attgatgtcg gtagccatga caatgatttc cctatccaga atatcaccat gccgtttggt
     3661 agctatggta tccaaaatat aattgtacaa tgtctcgact tcacgccttt tggaataatt
     3721 ggaagtaatg gagatcgtat catcatcaaa caatccggtg tcaaaatcgg gtcgatcaac
     3781 cgaattgtgc tggatggatt gttgaagaga agctaataaa gtggttccat tgttttgttt
     3841 taaaggcagg tcggtaatcg cattgataaa ttcctcatga ccggatatca acctgaatat
     3901 actttgaata accttacccc aatctgtcaa taagggatgt ccaacggtaa tatgttctat
     3961 tctcctgttt ttcgccttcc atttatccaa taccttttgt ggctcatctt caaaccaata
     4021 ataatcgggg gccggattat tcgcatacca acgaatatct atgtgttcac tcattgcatg
     4081 gatgatctcc aaatggtatt ttgtaatgat ggaaagaccg aaaagatgca aaaccggaat
     4141 attcctgacc agacgctcct gattttgtac ggttttgagt ttttccagga tatctacttt
     4201 tattgcatta aaatcaatga tcttatcacc ggcaatcgaa tcaatctttt tccataaata
     4261 ggcctgccag cgggacttct cttccacgct ttccaattca tttaccttcc attgtgcaat
     4321 aacttcactg cggtagatct gatactgatc aaacaaatcc cccatcttag ccgctaattc
     4381 ccattgtttc atcttatctc taccttcttc attgtaaaag taggaagctc tttcagggaa
     4441 cttttcttta aatgcttcgt cagacaaggc agcatatatc aaccaatcca tgtccttccg
     4501 gtcaatttta tctttatagc tgccgcccag aatcttatag acttcgtata ctatttcctc
     4561 cggtgtcaaa aacatcaggt tggcggccac accatgctgt tcagacattc tgtacttgag
     4621 ccaactgttc atgcccttcg tttgagtgac gatatagacc ggttggaaca catcaaacct
     4681 gtacaggccg atgtcctcaa tcatagcatc ggccaacttg gtaatatcat ttgaaatagg
     4741 tatgtgtatg cccataactt tatgatttaa aatggtcctt aataccggat acccgcttga
     4801 cggactgttc ggcaattgca cggatgattt cactaaagcc tacaatggtg actttttctt
     4861 ttgctcgtgt aatggctgta tacagcaatt cgcgtgtcag taaaatgttt tctctgttct
     4921 caggcaggat gatcaagact tcatcgaatt cagagccctg gcttttgtga atggtcatag
     4981 cgtaagccgg ttcccattca cggatctggg ccggattgag ctttagattc tcgccgttac
     5041 ctttaggaaa ataagcattg acctcacctt ttacttctaa aatcagaccc atatcaccat
     5101 tataaatacc ttcagaatgc tgattggaag caaccattac cgggaggaag ggataaaatc
     5161 gctcattatt gtgtgatacc tcagaggcaa tcattcgatt taccgccaat acgccagtct
     5221 tgccttcttt aactgcacac agcactatac tttttacaag tcgatttaat gcctcgccga
     5281 ctccggtctg tccgacatat ccaccattga cctgcaaaag cctctgagca gccgacttca
     5341 aagcgctttc cacatcattc gggcacagaa atttaaccct atcatcttta ttttcaagga
     5401 attctttaat aacaacctga tcgttgttca aaacagcctt gctgaacttc ccgattcctt
     5461 catcgtcact aaaacggaaa ctggtctgta gctcaatcat tctttcaaaa agaggatgtg
     5521 taacttctcc tgacatacaa tccaagggct cttcaataaa accattgata aaatcagtcc
     5581 ttaccttaga gaagatattc atcggtgcag tatcgttgca gaggtcccta aagatactgc
     5641 ctgcttcaac cgaagccaac tggttcttat ctcctaaaag caccactctg gttctgccgg
     5701 gatcaatcgc cttgaacagc ttgaaaaaca tcgtgatatc aatcatggaa cattcatcaa
     5761 cgatgaggat atcaaaatcc aatgggttgc cctcatgatg tttgaagtga atgctgttgt
     5821 ggatatatcc caacatcgaa tgcaatgtca gcgccttgag atcagcaatc ttttgaacca
     5881 attcatcatt gaattcactt cccgccaact tatctccggc cctggcaaag ctttcatcaa
     5941 ggtgtgcgct tgctttccct gtaggtgcac aaacacctat tttagcatcc ggagtctgtt
     6001 taagtaagag gtagagtaac ttggcgactg ttgtagtttt accagttccc ggaccccctg
     6061 aaataattgt aaatcgactt aaaaatgcag ttaagcatgc taccaactgc caatctgctc
     6121 cctttacgta gccggaagga ggaaacaagt gtctgacgat aaaatcctta ttatcgtgta
     6181 aaaaatcaaa atccctgtca gctgccacaa gctcacataa ttgcctgatt ttttctataa
     6241 gtgatttttc atattggaaa taccggttga tatacagtct tccattgtgg attacgaagg
     6301 gcttgttctc ctgaggagga ccgaccagta tatgatctga cagaatagat aaattgtgat
     6361 attgactttc ggaaaatgca tcaatatcta tacatatatt tcccaatacc atttgttcaa
     6421 tcaaagtcct taaccatggg aaaagatcat cttttgcaaa aaatccggca aaagcatcgg
     6481 ctgagtttat tgaactgatg tccatccaag ttaaatttat ggatttgcaa gatagtataa
     6541 ataaatatat atgtagaaat taacaagaag atatttaaat tttctacata tctaaatttt
     6601 atgtattcca atttctgctt tttgaaatta attcttattt ttatcgaaat ttaaaatcat
     6661 gcctaaatca tccgttttac acatcaccca cgattccttt gtcaaattgg tactttccaa
     6721 tccagctgca ggacaagact ttttaaatta ttttctacca gaaagtctta aggggaattt
     6781 ggatatagag aaatgtgtac cgagtgaatt gtcttatctc accgaatcaa tgcgtaatcg
     6841 ccacactgac ctgatttttg atgttcccat aaaaggctcc ggtgaaggaa tccgaattgc
     6901 aatattaatc gaacacaaat cgtataagga cgaagcagta attttccaat tgttggaata
     6961 tttagcttta ggttacagga agcaaagcag ggaaaaaaaa ggtttcaggt tgattattcc
     7021 actactctat tatcatggga aacaagaatg gagccccgcc ggtttgagtt cgtattttat
     7081 agatataccc ggcgatttga agccatacct acccgaattt agccggatat acatcgatct
     7141 caaaaattat tccattgatg atattgaacg attgaacaat tcattgttac gaaccgctct
     7201 atacattcaa ctattaagat tgcaaaatat ggaaaacaaa actatattga gcagggcatt
     7261 tacaaccttt gaatacggtc agtacaggaa ctattttagt gcaatatttg tttatgcgat
     7321 taaatatctg gacttggaaa aagaagaatt cgaaattttg gttgatgagt ttcccgaaga
     7381 ggtaaaaccc gaagctatga ccctcgcaaa tcgactgaag cgcgaaggga tggaggaagg
     7441 tattgaaata ggcaaaaaac aaggcatcga aaaaggaatt gaaaaaggga ttgaactatc
     7501 acaagtttct ttcgttttca agagttatga aaacggactc gatgcattga ccatcgctaa
     7561 aattgtcggc attacagaag accgtgtcag agaaattctc aacattgaaa gataatcaat
     7621 cggaatcttt atccaatgaa ttgatctttg ccaactactc catttatatc caatcgtatg
     7681 atacttgatt tcggcattcc aaggccttat ggaaattgag aggcaactta gatgattccg
     7741 gataaaaaat tcattctgaa aatatttcga taatatttca tatatttgat tttttattga
     7801 tcatgaaatt cccgattcag gtagaacaaa aaaattgtct caattgcgct ttcaagggcc
     7861 actcactgtt ttcgatttta tcggacgatt cgctcgatca tctcaacgcc cacaagtctt
     7921 gcactatttt caagaaaggt caatatcttt tcacccaaca cagtcgacca atgggtctat
     7981 tttgtgtata ctcaggtaag ataaagctct ctacagccgg tcctgatgga aaggaacaga
     8041 ttatccggct ggctaaggat ggcgatgtaa taggctatcg tgcaatcatt acggatgaaa
     8101 attacagact ttctgctata gcattggaag aaagttcagt gtgcctgatc gagaaggaat
     8161 tttttctcga tttagccatg agtgaaccca ggctgaacag gcaaatattc aaattaatca
     8221 gcgatgattt aaagagagcg gaggaacaaa ttgtttcctt gtcacaaaaa agtgtgcgcg
     8281 agcgtactgc tgaggcactt ttattcttaa aagcaactta tggctttgaa gatgataacc
     8341 aaacactcaa tatccgccta agtcgggaag aattggccga ttatgtaggt acgactactg
     8401 aaagcgtaat aagggttctt tcagatttca aaacaagtgg cttaattgat atcgccgcta
     8461 gaaaaataat tatcattgat ttgggcctgc tgattaagac tgccaatatg attccttagg
     8521 ctgaatgcga tacaagcatt tgattttcat aagcttcgga ttaccttatc agacactttc
     8581 aatttatatt caccgctttc caccttcaat agcttataca ataaaacaaa agcctgttgg
     8641 agcaacacct tgcttcactt tctgataaat tcctgattgg caggataaaa tatactattc
     8701 caacaagaag actttcgaag aaatgtcaga cttatatgat attgagtatg taaagtaaaa
     8761 ctatacaaga gaaagataaa attaaaaaaa ttaaagcgat caccgccaaa gcttaaagcc
     8821 tacccgattg attatgtggc ttttacaaca tttatccaaa ctccgacaaa ttaaaattta
     8881 tcagatatga cttttagttt gttgaaaatt acttactttt accggaaata ttttacctta
     8941 tgaacatatc taagtgtgtt attctaaccc tggggataat gtctttactg agttgtaaaa
     9001 atgactccca taccacagaa catggctcga atgaatcatc tcatgccgct catgtttcta
     9061 caattgacag cacccaaccc cctatgttca acgaaatcat ggcagtacat gatgagatta
     9121 tgccgtggat gaacaatata gaaacactaa agcaggatat ccggatgaaa atggattcca
     9181 tagatgcctt tggaggtagc gatgccaaca gaaagcaatt cagacatgct atggcagagc
     9241 tcaatagagc tgacaacgca atgactgatt ggatgtcaag ctttaaggaa aactatgata
     9301 ccattcagat agaaaaaaac aaaatggtat tccttgaatc ggaaagagct aaaattgagg
     9361 gtgttaaaaa tcttatgaca gaaagtatca aggtggctca attgaccttg aaaaatacac
     9421 ccataaaata aatacgctct caaggcatta tgacattcag atttttcatt ttctctttat
     9481 tgcttctggt gcttaatggg tgcaatacca agcccgaacc attacccttc ctcggtataa
     9541 aagtacatga ggatgataag cctcaaattc cggaatatca ttttatcgat cagcttaacc
     9601 gcccggttac gcctgagact tttaaaggca aaatatatat gactgacttc tttttcacct
     9661 cctgtcctac aatttgcccg attgtaacag gtcaaatgct ccgcatatat aaggagtttg
     9721 aaaaagatga tcgggtggcc ttcctttctc attccataga tacccgacat gactcggtac
     9781 ctaaactgaa ggcctatgcg gataagctgc acatcaatga tgacaaatgg agattcatta
     9841 ccggcaagaa agaagacatc ttcaacatct caaagaatta cctgagtatg gtgctggaag
     9901 atccaaatac tccgggaggg tttgatcact ccggatatat tatccttgtc gataaagaca
     9961 ggcatgtacg tggcttttgc aatggaaccg acccaaagga tgttgataaa tttatgggca
    10021 aggtgaagca attacttgat actgaatgac ccggtggctg attgtacttt tgtcagctgt
    10081 ttttgttctt tattcaggct gcaccacgta ctcccaaggc aagcgaatct atgagtcaaa
    10141 atgcgccaac tgtcacggaa tcaaagggga aggtctagca gcactctatc cggcacttga
    10201 taaatccatc atcattactc atcataaatc acggattccc tgtattatcg aactcggcag
    10261 gagttccaga gacagtctgg gaaacctaac ttcagaaatg cctcctgtca aaggactgac
    10321 tgtagctgaa atcactaatt tgatcaatta cctgctaaca ttgaacccca atggacagga
    10381 aacagtaacc attgattcag tcaaggtatg gagtgaggct tgcaattaaa tattttttct
    10441 tatcgcaaga aataattatg tttacatctg ttttttaaat gaattatatg ttcttaagat
    10501 tatctttaat aggcctaatt tcaatctttt ccctgttttt aacaaatcaa atacttcttg
    10561 ctcagaattc aattggaata gatatgaaat atgatgccgt agggaaaata tttgttgaaa
    10621 actgtgaagc tactaagggt acaatagttt ttatatttga taatcaacag gttgaaaggc
    10681 gttttgaagt aaaatttaca ggagatgcga cacccggcac tgattttcag gtcgatggac
    10741 ttagtgatac tcttgtagtg ggctccggaa ttcaaactat tgaattgcca tttacagtga
    10801 atgtagataa tgtggcggaa ggaagggaag acctgacaat caccctggtt tcaattcctg
    10861 aaggtcagga aattgcaagc tacagtacaa gtatatatga tgaatttagt gttagtatca
    10921 atgacggtgt gcaggtagaa agctgtaaag gtcaaaagtt actcctgaca gcctccgaag
    10981 caggaacatt tttatggaca ccttccgaaa actttactga tgcaaccaaa caaactgtcg
    11041 aatatgtcag caatgaatca tccacagttc atctgactgc aggcctgggt tcttgcacag
    11101 ctaatgcaac catcaatgtc agcatcatca atacagatgt attattaact gctgataagg
    11161 ataccatttg cggtccttcc cttgtaacat ataccgccac cgctgctaca cagggaggtg
    11221 tctttacatg gtcccctgcg gaactgtttc ccaatcagaa ttcagccact caaagtgtct
    11281 tgatcaaagc caatacacaa gtcaaagtga gttatagcaa aggcgattgc acttcatc
//
LOCUS       DAOQQN010000050        11284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2046_length_11284_cov_5.765073, whole genome shotgun sequence.
ACCESSION   DAOQQN010000050 DAOQQN010000000
VERSION     DAOQQN010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11284
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2046_length_11284_cov_5.765073"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="PLG24_03090"
     CDS             complement(<1..166)
                     /locus_tag="PLG24_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HMZ23901.1"
                     /translation="MKRAILAYGHSILKQKCKDIEKDYPELDKLITDMWETMKNANGC
                     GLASPQIGLLI"
     gene            complement(223..789)
                     /locus_tag="PLG24_03095"
     CDS             complement(223..789)
                     /locus_tag="PLG24_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ23902.1"
                     /translation="MGHIREYYERIIKLQESEWEFIASHFERKVFAKNEIITRQGETE
                     NFLSFVETGIVRFYIPDVENELTFNFSFDKEFTCAYDSFLTQTPSEYELQALTETVVW
                     QISFDDLQKVYAQTKVGNYLGRFASEKLFLAKSKRELSLLKYNAKERYLRLFSEQPDI
                     LKFIPLKFVASYIGITPQGLSRIRRQIT"
     gene            complement(1150..2229)
                     /locus_tag="PLG24_03100"
     CDS             complement(1150..2229)
                     /locus_tag="PLG24_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HMZ23903.1"
                     /translation="MDFKDQIKQLGDRVTKLKEQILTEEATKNAFIMPFLQTLGYDVF
                     NPLEVVPEYITDIGTKKGEKIDYAIFKEGNPTILVECKHWGQNLNLHDGQLLRYFHVS
                     KAKFGLLTNGITYRFYSDLVESNKMDERPFLEFNITEIKDNQIEELKKFHKTIFDAES
                     ITNTASELKYTNELKQLLQQELTNPSADFVKHFARQVYPSVVTAKVLEQFTNLTKKSI
                     QQHISDLITERLKTALTKEDEKVKEQEAVQAEQVKADENKIVTTEEELEGFMIVKTIL
                     RQRIDANRITHRDAQSYFAILLDNNNRKTICRLYLNSSKKYFATLDEQKKEVRNEITS
                     LDDIFKYSELLLKIVASYDKSKENT"
     gene            2452..2604
                     /locus_tag="PLG24_03105"
     CDS             2452..2604
                     /locus_tag="PLG24_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23904.1"
                     /translation="MSHPSGKNDSNPPKRNTGLHPGWLNGKYSKAGLAMWLKMEDFGR
                     AFVYSD"
     gene            complement(2622..2969)
                     /locus_tag="PLG24_03110"
     CDS             complement(2622..2969)
                     /locus_tag="PLG24_03110"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HMZ23905.1"
                     /translation="MQINQYEIWIADLNPQIGTEAGKTRPVLVVQTNLLNNIPHPSTI
                     VCPITTNVQKDSDILRVHIKKGMANLHENCDVMIDQIRAIDNKRLVKKVGSLPNELID
                     RVKENIAIVIDLE"
     gene            complement(2959..3189)
                     /locus_tag="PLG24_03115"
     CDS             complement(2959..3189)
                     /locus_tag="PLG24_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23906.1"
                     /translation="MKTVSLKIEDSIFGETEKILSRISISRNRYINEAIEYYNKIQKR
                     QILEKKLKIESNLVKKDSLNTLKDFENIDYAD"
     gene            complement(3341..3580)
                     /locus_tag="PLG24_03120"
     CDS             complement(3341..3580)
                     /locus_tag="PLG24_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23907.1"
                     /translation="MPFRETSCSAVFFLGASLSFLPDGWLTFVGQYRKIDLVFCMEST
                     AVPQRMEVTLFGNTSLDVAHFTINPPHRHGPTRPA"
     gene            complement(3658..3969)
                     /locus_tag="PLG24_03125"
     CDS             complement(3658..3969)
                     /locus_tag="PLG24_03125"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ23908.1"
                     /translation="MSNKMTNKKLTSFSDHLDSQYGKKGTEKREQYEEEFEAFKLGVM
                     IQELRKEQGLTQEQLAEKCGTTKTYISRIENNASDIRLSTLMRIIRQGLGRNLKLNVT
                     H"
     gene            complement(4112..4303)
                     /locus_tag="PLG24_03130"
     CDS             complement(4112..4303)
                     /locus_tag="PLG24_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23909.1"
                     /translation="MNEKFIRQIFYYENYYLNFFETLKPEVKKKFNWTLQLIATIDRV
                     PKKYFDRMTNTSGLFEVRV"
     gene            complement(5901..6755)
                     /locus_tag="PLG24_03135"
     CDS             complement(5901..6755)
                     /locus_tag="PLG24_03135"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HMZ23910.1"
                     /translation="MKNNIFRLCILTGMALGLWSCQETEIVVPRVKEIVSDRVVLIED
                     FTGVQCTNCPNASREISSLAHKYNGNVVAIAYHTDFLGLPITKDGYKSKYNFTTPDGN
                     LLEEEMGGYQGKPAVTLNRKLYDAQQEYLLTSSSILGNIESELRSIPRVVIDIENQYD
                     AETRKLRTKVDIKPQAVASGDFRLHVCITEDKIIDSQIDNIVYVKDYEHNHVFRTMLS
                     TLAGDILGTDLVPGSNLSRTYEYTLPPEAGWWVASHCNVVAFVTDLSQKAFNVGAVLQ
                     AAEKHVIE"
     gene            complement(6766..8415)
                     /locus_tag="PLG24_03140"
     CDS             complement(6766..8415)
                     /locus_tag="PLG24_03140"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HMZ23911.1"
                     /translation="MQKLALTFFGFFLVLWQGYGQGITGGKVSGGLQTNLNFFMKDAS
                     INAANTPQYETQLYSADSWLNVNYNTKGFEAGIRLDVYNHSYLFNPNGGSYTGIGIGN
                     WYVAKQIDKLNIAGGYLYDQIGSGIIYRAYEERPLAIDNALFGVRASYDLTPNWRVKG
                     FTGQQKFQFDRYGAIIKGASIDGFITVSDSAAKHTLTLSPGAGVVARTFDNPTIEGIN
                     NVLKYYEPIDSIATYKSNTYAMTVYNTLSYGPFTLYTEAAYKTPEVMYSPTEFRHIGL
                     DSSAGKFIKAAGTVFYGSLSYADHGLGINIEGKRTKNFSFRTDPNLLLNRGLINYIPA
                     MSRVNTYRLTSRYNPAIQFMGEYAFQVDVQYRFNDHWNALINYSHINDLNDVKLYREI
                     YGEVTFKPNKKNQFILGLQRQEYNQYIYEGKVSVPNVETFTPYIEYLHKFNRKTAIRV
                     EGSMMATRQDYGSWAFGLVELTIAPHWSFVIADMYNYDYNPEKTKKANNYPSIIAFYT
                     RGSNRFSLGYIKQVEGIVCTGGICRYEPAFSGVKATVSTTF"
     gene            8684..9190
                     /locus_tag="PLG24_03145"
     CDS             8684..9190
                     /locus_tag="PLG24_03145"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMZ23912.1"
                     /translation="MRFQNLYLTFMMLFVIGVSTQAFGQTDLPSTRVKTLDGKSVTLQ
                     EVIKPGVITVISFWATWCAPCKKELDAINEIYEDWQSEYGVELIAISTDDARTAPKIK
                     PTITQKGWKYTFLNDQNQELQKALNFQAVPQTFLIGKDGKIAWTHAGYVPGDENELEN
                     HIKELAGK"
     gene            9201..9809
                     /locus_tag="PLG24_03150"
     CDS             9201..9809
                     /locus_tag="PLG24_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23913.1"
                     /translation="MNKLLHPGLRKKPFFFLLLFISMTAMSVISCKQDDGTETKVTVD
                     AAPELIAKKDRMKGYIDSVFLTRSEAEDSLYTTRMLYEDAKAIATGMKQDTSVPEVLF
                     RAGALASGRKDPTKAIGIWGLITSEFPESRWASEAAFQKAFTFDNDLHDKETAKKNYE
                     EFLKDYPNHKLAGDVKLLLQTMNKTDAQLIEEFEKKNNIKSN"
     gene            9829..11001
                     /locus_tag="PLG24_03155"
     CDS             9829..11001
                     /locus_tag="PLG24_03155"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMZ23914.1"
                     /translation="MRIKFLAIVLMVNCTVLSFAQGIEFFHGTWEEVKAKAKEADKLV
                     FVDAYTTWCGPCKRMAANIFPLPEVGEFYNANFINYKLDMEKGEGPDFARKYGVRAYP
                     TFVFVDGDGNMVFNRVGGSDAPGFIQMGKTAVDKFDKSPALAKKYEGGDRSPSLVLDY
                     IKALNAAGKPSLKIANEYLANEKDTKSDLYYKIIFESALSSDSRPFSLLISNEKAIAK
                     LVGKQAYDKRVYNALIHSVQKGIEYDTEDLITEAATTARKVLDKDNARAFPNHVDVLK
                     ADAANDGKLYISRVAELNKNLSSGTLIDLSGIIKTLQSKYSHLDNAQSILGVLYKKLA
                     DYSDDPDKRLDYAMYLSGTGDKSGALKEANKAKEDAQKKGIDTTKYDNTIIKINSK"
     gene            11009..>11284
                     /locus_tag="PLG24_03160"
     CDS             11009..>11284
                     /locus_tag="PLG24_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HMZ23915.1"
                     /translation="MKYAKILTFALIALMVQSGLGQGMTFETGNWASILKKAKKEKKL
                     IYLDAYTSWCGPCKMMKKNVFPDAAVGEYFNANFVNAQIDMEKGEGPG"
BASE COUNT         3192 a         2279 c         2248 g         3565 t
ORIGIN      
        1 ggatcagaag cccgatttgt ggtgaggcta atccgcaacc attggcattt ttcatcgttt
       61 cccacatatc tgtaataagt ttgtccaatt ccggataatc tttttctatg tctttgcatt
      121 tctgtttcaa aatgctgtgt ccgtaggcta aaattgctcg tttcattctt ttgatttttt
      181 gcaaagttca gaagaagaaa cagcataggc aatgaaccga tgttaagtaa tttgtctgcg
      241 tattctgctc aatccttgtg gtgtgattcc gatgtaagaa gccacgaatt taagtggaat
      301 gaatttcaga atgtcgggtt gctcgctgaa caatcttaaa taacgctctt tcgcattgta
      361 ttttagcaga gacaattctc ttttgctttt ggctaaaaac aatttttcgg aagcgaaacg
      421 ccctaaataa tttccgactt tggtttgtgc atacactttt tgtaaatcgt caaaagaaat
      481 ttgccaaacc acagtttccg tcaatgcttg taattcgtat tctgagggtg tctgtgtcaa
      541 aaaagagtcg taggcacaag taaactcttt gtcaaagcta aagttgaaag tcagttcatt
      601 ctcgacatca ggaatataaa accgcacgat acccgtttca acgaatgaaa gaaaattttc
      661 cgtttcacct tgtcgagtga tgatttcgtt tttagcaaaa actttccttt caaaatgaga
      721 agctataaat tcccattctg attcttgcaa ttttattatt cgttcgtagt attctctaat
      781 gtgtcccata tttgttggtg tcaggttttt tgtttgccac taacagctgc atatacgcca
      841 tctcacgtat attttctgta taaatgtaat cctaatcctc aaacaaccaa actatccaac
      901 gcccttttta tatcctatct cagtttaccc tttacattcg agtaaatctc agttggatat
      961 tccggagtaa agtgagcgat ccatccggca aaaatgatag taatccacct aagagaaata
     1021 cagtccttca tcaggtctcg ctgaatggca aatattcaaa agccgggctg gtttggtgat
     1081 taggttggaa gatttttgat gggctttggt atatttggct taatgttggc attagtggtt
     1141 ttctatgtgt tatgtatttt cttttgattt gtcataactg gcaacaattt ttaatagcaa
     1201 ttctgaatat ttaaatatat catcaagcga tgtgatttca ttcctcactt cctttttctg
     1261 ttcatcaagt gttgcaaaat actttttaga actatttaaa taaagcctgc aaattgtctt
     1321 acgattattg ttgtcaagta aaattgcaaa atatgattga gcgtcacgat gagttattct
     1381 gttggcatca attctttgtc gaaggattgt ttttacaatc ataaatccct ctaattcttc
     1441 ctctgttgtt acaatcttat tttcgtctgc ttttacttgt tcggcttgaa ctgcttcttg
     1501 ttcttttact ttctcgtctt cttttgttaa tgccgttttt aaacgttcag ttattaagtc
     1561 gcttatgtgt tgttggattg atttttttgt caggttggta aattgctcca aaacttttgc
     1621 tgttacaaca cttggataaa cttgtcgtgc aaaatgctta acaaagtcag cactcggatt
     1681 tgtaagttct tgttgtaata gctgtttcag ctcattagtg tattttaatt cacttgctgt
     1741 attagtgata ctttcggcat caaaaattgt tttgtgaaac tttttaagtt cctcaatctg
     1801 attgtctttt atttcggtta tgttaaactc taaaaatggc ctttcgtcca ttttatttga
     1861 ttctaccaag tcggaataga accgataagt aatgccattg gtaagcaatc caaactttgc
     1921 ttttgaaaca tgaaagtaac gaagtaattg gccatcatgt aagttgagat tttgacccca
     1981 atgcttacac tcaactaaaa ttgttggatt tccttcctta aaaatagcat agtcaatttt
     2041 ttctcctttt tttgttccaa tgtctgtgat atattctgga acaacctcaa gtgggttgaa
     2101 aacgtcataa cccaatgttt gtaagaacgg cataataaaa gcattcttcg ttgcctcttc
     2161 tgtcagaatc tgctctttta gtttggtgac cctgtcaccc aattgtttga tttggtcttt
     2221 aaagtccata cttattcttc atttttaatt gatttttctt ttgagtaact ttaatggtgt
     2281 gtatcgtcct accattactg caacatctgc ttatacacaa tatgccgtat attttccgta
     2341 taaaagtaat cctttcactc aaatatccaa tctatccaac gggcttttta taccctttct
     2401 cagtttaccc gttatattcg tgtaaatctc cgttggatat tccggggtaa aatgagccat
     2461 ccatccggca aaaatgatag taatccacct aagagaaata caggccttca tccgggctgg
     2521 ctgaatggca aatattcaaa agccgggctg gctatgtggc ttaagatgga agattttggg
     2581 agggcttttg tatattctga ttaatgctgg gcgttcgtgc tttattctaa gtcaataact
     2641 attgctatat tctcttttac tctatcaatt aactcgtttg gtaaactacc aacttttttg
     2701 accaatcgtt tgttgtcaat tgcacgaatt tgatcaatca ttacatcaca attctcatgc
     2761 aggtttgcca ttcctttttt tatatgaact cttaatatat ctgaatcttt ctgaacattt
     2821 gtggtgattg gacatacaat tgttgatgga tgtggaatat tattgagtaa atttgtttga
     2881 acaactaaaa caggtcttgt ttttcctgct tcagttccta tttgtggatt tagatcggcg
     2941 atccaaattt catattgatt aatctgcata atcaatgttt tcaaaatctt taagagtgtt
     3001 caatgagtct tttttaacca ggtttgattc aattttaagt tttttttcta aaatctgtct
     3061 tttttgaatt ttattgtagt attcaatagc ttcatttatg tacctatttc tcgaaatact
     3121 tatacgtgaa agtatttttt cagtttcgcc gaatattgaa tcttctattt ttaaggatac
     3181 ggttttcata tactatttgt ttatattaca aaggtatatc ttttttgtat ataatatttt
     3241 gttttacgta atttttttta gcatgacgca caattcaaag gaatattgat actctcccct
     3301 tttcaaccct taaaagtgat tctaatcaag tatcaatagt tcaagctgga cgagtcggac
     3361 catgcctatg tgggggatta attgtaaagt gagccacatc gagtgaggta tttccgaaca
     3421 aagtgacctc catgcgctgt ggcactgccg tactttccat acagaagaca agatctatct
     3481 tcctatattg accgacaaaa gtgagccatc catccggcaa aaaagatagg gatgcaccta
     3541 agaaaaatac agcactacag ctggtctcac ggaatggcaa atatttaaaa gccgcctggc
     3601 ttagtggctt attttggaag attttgtata ttcgggtaat gttgggcggc agtaaattta
     3661 atgagttacg tttaatttta aatttcttcc taatccctgt cttataattc tcattaatgt
     3721 agaaagtcta atgtcgcttg cgttgttctc aattcgggaa atatacgttt ttgtcgtacc
     3781 acatttttcg gcaagttgtt cttgtgtcaa gccttgctct ttacgaagtt cttgtatcat
     3841 tacaccaagt ttaaaggctt caaattcttc ttcgtattgt tctctttttt cagttccttt
     3901 cttaccatat tgactgtcca aatggtctga aaaagaggta agttttttat ttgtcatctt
     3961 gttgctcatc gaaatattgt tttttaagtt tttctgctaa ttcaatttca ttcttcggag
     4021 ttttctgtgt tttcttctga aatccgttaa tcaacacaat cagatttcct ttgtcaaaaa
     4081 agctgaaaac tctgtaaata tcagaaccga cttaaactct cacttcaaat aagcccgaag
     4141 tgttggtcat tctgtcaaaa tactttttcg gaaccctatc tatggttgct atcagctgta
     4201 aagtccagtt aaacttcttt tttacttcag gctttaatgt ttcaaagaag ttgagatagt
     4261 agttttcgta ataaaatatt tgccgtataa acttttcgtt cattagctga tgtcatttct
     4321 aattttttta caaagttagc taattagata acaataacca atattagaag gcataatttt
     4381 taggcattga gtggtctgtt cgtatagttg cagctcaaag gaatacttat accctcccct
     4441 tttcaacccc aaaaaaagta aatcaaaaag tatcaatatt tcaagctgga cgagtcggac
     4501 catgcctatg tgggggatta attgtaagtg agccgccccg gggattggga tttccggaac
     4561 aaagtgcgca ccatgcgctg tgggcttgcc atgccatcca atccggcaga caagttctat
     4621 cttcccactt tgaccgacaa aagtcagcca tcaatccggc aaaaaagtta ggaatccacc
     4681 taagagaaat acagtccctc atccggactc gctgaatggc gaatattcaa aagctgcccg
     4741 gtttagtgat ttaatttagt aaacttttgg tggacttttt ttattcggct taatgttagt
     4801 gactgttttt attcgttcaa cagccaatca attacgttca gttttcgaat accgtctatg
     4861 cttgtattgt caaaatcgag agttaaaatg tatttcgggt agttgtcttt gatgtttcta
     4921 aatgccgaaa cttctctttt gtacgttttt tcgtcattaa gagtaaaacc aacctgtatt
     4981 gaagtccttg gtataaagtt ctaaactccc aaatgcaatt actgagcttt ttgaataatt
     5041 cggggtcatg ttcggtctgt gccttacgaa tgttttaaag tattttttcg taatgctttc
     5101 tatctaatcc tttcaagagc tcaaatgctt cgtctaaaaa tattacttca aaaagtttgc
     5161 ccattcgtta aattgccaac aaagcaaaga caataaaagt ttcgttataa tgaaacattg
     5221 ggtcgattta ttttgtcatg attggtcggc aaatatggca tagaacggtt tcgggctttg
     5281 agcaattgtt cggtggattg ttggttggta ataatatttt cttttaattg ttgggtaaag
     5341 gcaatatcct ttttttcaac taccccaacg gggtatcatg tttatagaaa catgattata
     5401 atgaatgtgc gaccctgtag gggtcgaata aaattgtttt acaatttttc tataaacatt
     5461 aaatcccttc aggattatgc gtattccgtt tccgtcacat caaatgcctg atgtagcaag
     5521 gcttttaaca attgttcgct ggattgttgg ttggtaataa tattttcttt taattgttat
     5581 gtaaggacaa tatccttttc ttcaactacc ccaacggggt atcatgttta tagaaacatg
     5641 attataatga acgtgcgacc ccaacagggg tgtgatgcaa taggacaaat gcaatttcta
     5701 tatcattcaa tggggcatca atggaaagtt ggatttcatc cctcatcagt ctgtacagta
     5761 tggacaatgc aaatgaagcc ggattgaata cccaccccct tggcaggtgt caaccccata
     5821 accaaattca aactttcttt atttgaaaaa aatatttagc tcatcctgcg gacgacatat
     5881 cagtctcgac cgctaatgcg ctattctatt acatgctttt cagctgcctg cagtactgcc
     5941 cctacattga aagccttctg tgacaagtca gtaacgaatg ccacaacatt gcaatgggac
     6001 gctacccacc aacccgcttc cggcggaagg gtgtactcat atgttctgct gagattactg
     6061 ccaggcacca gatctgttcc gaggatatca cccgcaagtg tgcttaacat cgtcctgaag
     6121 acatggttgt gctcatagtc cttgacatat actatattgt cgatctgaga atctatgatc
     6181 ttatcttccg tgatgcatac atgaagccgg aagtcgccgc tcgccacagc ctgcggtttg
     6241 atatccacct ttgtgcgcaa cttccgcgtc tctgcatcgt attggttttc aatatcaatg
     6301 actaccctgg gtatacttct caattctgat tcgatattgc ccaaaataga ggatgatgtg
     6361 agcagatact cttgctgagc atcatacaat ttcctgttaa gagtgactgc cggcttgccc
     6421 tgatagccac ccatctcttc ttccagcaag ttgccgtccg gtgtagtaaa gttgtattta
     6481 ctcttatatc cgtccttggt gattggtagc ccgaggaagt cggtatgata agcgattgcc
     6541 accacattac cattgtattt atgtgcaaga ctggagatct cccggctggc attcggacaa
     6601 ttggtgcatt gtactccggt aaaatcctcg atcaatacca ctctgtcgga tacaatctct
     6661 ttgacacgag gcacgacgat ttcagtttcc tggcagctcc aaagtccgag tgccatacct
     6721 gtcagaatac atagtctaaa aatattattc ttcattttga aaaaattaaa aggtggtcga
     6781 aacagttgcc ttcacgccgc tgaatgccgg ctcataccga catatacctc ccgtacatac
     6841 aatgccctcc acctgcttga tatagcccaa tgaaaagcgg ttgctgccgc gtgtataaaa
     6901 ggcgataatg gacgggtaat tattggcttt ttttgttttc tcggggttgt aatcataatt
     6961 gtacatatca gcaatgacaa aactccagtg tggcgctatg gtcagctcta ccaggccaaa
     7021 tgcccagctt ccatagtcct gcctggtagc catcatcgag ccctctacgc gtatggctgt
     7081 cttgcgatta aatttgtgta gatactctat ataaggggtg aaggtctcta cattgggcac
     7141 gctcaccttg ccttcatata tatactgatt gtactcctga cgctggagac ccaggataaa
     7201 ctggttcttc ttgttgggct tgaaggtcac ttctccgtat atctcccgat atagcttgac
     7261 atcattgaga tcgttgatat gagaataatt gatcagggca ttccaatggt cattgaatct
     7321 atactgcaca tcgacctgga aggcgtattc tcccataaac tggatggcag ggttgtatct
     7381 ggatgtcagc cgataggtat ttactctgga catcgccgga atatagttga tcaggccgcg
     7441 attgagcagc aggttaggat cggttcggaa tgaaaaattc ttagtacgct tgccttcaat
     7501 attgatgccc agaccatggt cggcatagct caaactgcca tagaatacag ttccggccgc
     7561 cttgatgaat ttcccggctg aagaatccag gccgatatgt cgaaattcag ttggagaata
     7621 catgacttcc ggtgttttgt acgctgcctc cgtataaagc gtaaagggac cataactcag
     7681 cgtattgtat actgtcatcg cataggtatt ggacttatag gtagcaatac tgtcgatggg
     7741 ttcgtaatat ttaagtacat tgttgatgcc ttcgatggtc gggttgtcga atgtcctggc
     7801 tacaacaccg gcacccgggg acagagtaag cgtatgcttg gctgcgctgt cgctgaccgt
     7861 gataaaccca tcgatgcttg cgcccttgat gatggcccca taacgatcaa actggaattt
     7921 ctgctgtcct gtaaaaccct tgacccgcca gttgggtgtc agatcatacg atgcacgtac
     7981 accaaacagg gcattgtcga tggcaagggg acgctcttca taggcccggt agatgatgcc
     8041 tgatcctatc tggtcataca ggtatccgcc ggcaatattc agcttgtcta tctgcttggc
     8101 cacataccag ttgccgatac cgatgccggt ataagagccg ccattgggat tgaacaagta
     8161 agaatggtta tatacatcca gtcgaatccc tgcctcaaac ccctttgtgt tataattgac
     8221 attgagccag ctgtccgcac tgtaaagctg agtctcatac tggggtgtat tggcggcatt
     8281 gatggaggca tccttcatga aaaaattcag gttggtctgt agccctccgg ataccttgcc
     8341 gccggtaatt ccctgtccat atccctgcca taacaccagg aaaaaaccga aaaaggtcaa
     8401 cgctaatttt tgcatctgag gttgaataaa attttaatgt ttgctgcaaa agtatgcttt
     8461 tatttcttct aagccattta caaaacaata aattattgac aaaaaatgaa gtagctttgc
     8521 gccgtccatt gagaataaag gggttgtttg tcttaatata cgtgtgaaaa ttggttaaag
     8581 tctagttaaa cacactgcat tgagtaaaaa ataaacgttc tttatggttt aatagatcga
     8641 tatttttttg cattttttaa ttaaattaaa aagaaaggat acaatgcgtt ttcaaaatct
     8701 ataccttact ttcatgatgc tgtttgtaat tggtgtaagt actcaggctt tcggtcagac
     8761 cgatctccca tcgacccgtg tcaagacact cgacggcaaa agtgtgactc ttcaggaagt
     8821 aatcaagccg ggagtcatca cagtcatttc gttttgggcc acatggtgcg caccatgcaa
     8881 gaaagagttg gatgccatca atgagatata tgaggactgg cagagtgaat acggggttga
     8941 gctaatcgcc atttcaaccg atgatgcacg tactgcacct aagatcaagc cgaccataac
     9001 ccaaaaaggc tggaaatata ccttcctcaa tgatcaaaat caggaattgc agaaagcact
     9061 caattttcag gcagttcccc agactttcct gataggtaag gatggtaaga tcgcctggac
     9121 gcatgcagga tatgtaccgg gtgatgaaaa tgaactggaa aaccatatta aggaattagc
     9181 cggaaagtaa ccttgtattg atgaataaat tattacatcc cgggcttagg aagaagcctt
     9241 ttttcttttt actcctcttt atttccatga cagcgatgtc tgtgatatcc tgtaagcagg
     9301 atgacgggac agaaacaaag gtgactgtag acgcagcacc tgaactgatc gctaaaaaag
     9361 acaggatgaa gggatatatt gacagtgtct tcctgacacg atcagaggcg gaagactccc
     9421 tttacacgac caggatgctg tatgaagatg caaaggcgat agccacaggc atgaaacaag
     9481 acacgtcagt acctgaagta ttgttcaggg ccggagcatt ggcaagtgga aggaaagacc
     9541 ctaccaaagc aatcggtatt tggggtttga tcacatccga gtttccggaa agtcgctggg
     9601 catcggaagc agcgtttcaa aaagcattta ccttcgacaa tgacctgcat gataaagaga
     9661 ccgcaaaaaa gaattatgag gaattcctca aagactatcc aaatcacaaa ctggcaggtg
     9721 atgtgaagtt gttgttgcaa actatgaata agacggatgc tcagctaatt gaagaatttg
     9781 aaaagaaaaa taatatcaaa agcaattaaa taagaacaca tctaatccat gagaattaaa
     9841 tttttggcga ttgtcctgat ggtgaattgt acggtactgt ccttcgccca gggtattgag
     9901 ttcttccacg gcacatggga agaggtaaaa gctaaggcta aggaagccga caaacttgtc
     9961 ttcgtcgatg cctatacaac ctggtgtggc ccctgcaaac gcatggcagc caatatcttt
    10021 ccgcttccgg aggtaggtga gttttataat gccaacttta tcaactacaa attggatatg
    10081 gaaaaaggag aaggacccga ctttgccaga aagtacggcg tacgagccta tcccaccttt
    10141 gtattcgtcg atggtgacgg aaatatggtg tttaaccggg ttggcggatc tgatgctccg
    10201 gggtttatcc aaatgggaaa gaccgctgtt gataagttcg acaaaagtcc cgccctggct
    10261 aagaaatacg aagggggcga tcgcagccct tcactcgtac tggattatat taaggcactc
    10321 aacgctgcag gcaagccgag cctaaaaatc gccaatgaat atctggccaa tgaaaaggat
    10381 accaagtcgg acctgtatta caaaatcata ttcgaaagtg ctctaagttc tgactccagg
    10441 cccttttccc tgctgatttc aaatgaaaag gcaatcgcga agttggtcgg aaaacaagct
    10501 tatgataaga gagtatataa tgcactgatc cattcagtcc agaaaggaat cgagtatgat
    10561 accgaggatc tgatcacgga agctgccacc actgcaagga aggtgcttga caaggacaat
    10621 gccagagcct tccccaacca tgtcgacgta ttaaaggctg acgctgccaa cgacggcaag
    10681 ttatatatct ccagggtggc cgagctcaac aagaacctaa gctcagggac actgatcgat
    10741 ctttccggta tcatcaagac actccaaagc aaatacagcc atctggacaa tgcacaatcc
    10801 atcctcggtg tactctacaa aaagctggca gattatagtg acgatccgga taaaagactt
    10861 gattatgcca tgtatctctc cgggacaggt gataagtcag gcgccttgaa agaagcgaat
    10921 aaggcaaagg aagacgccca aaaaaaaggc atcgacacta caaaatacga caataccatc
    10981 atcaaaatca attcaaaata atttaaacat gaaatacgcc aagatactga cttttgcact
    11041 gatcgccctc atggtgcaat ccggattagg gcagggaatg accttcgaaa ccggcaactg
    11101 ggcctccatc ctcaagaaag ccaaaaagga gaaaaaactc atataccttg atgcctacac
    11161 ttcctggtgc gggccgtgca agatgatgaa aaaaaatgta tttccggatg ctgccgtcgg
    11221 tgaatatttc aatgccaact ttgtcaatgc tcaaatagac atggaaaaag gcgaaggccc
    11281 tggt
//
LOCUS       DAOQQN010000051        11156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2087_length_11156_cov_4.670840, whole genome shotgun sequence.
ACCESSION   DAOQQN010000051 DAOQQN010000000
VERSION     DAOQQN010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11156
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2087_length_11156_cov_4.670840"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PLG24_03165"
     CDS             <1..479
                     /locus_tag="PLG24_03165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ23916.1"
                     /translation="KEIDISNAGIFFLFLASGIILSRVTSGKIVDKGYIHIVMMVAIV
                     TISVGFTCFAMLHNIFAYASSAFVLGIGYGTLFPALQTIYIDMAPASRRGTANSTYLL
                     GFDLGIGIGMLLGAILEVRYGFQSMYLMTAGFCILSLIMYWTVSRKIFERYKLEKD"
     gene            complement(805..1272)
                     /locus_tag="PLG24_03170"
     CDS             complement(805..1272)
                     /locus_tag="PLG24_03170"
                     /inference="COORDINATES: protein motif:HMM:NF033196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ23917.1"
                     /translation="MAFPVNKKFIGTILASAMILFSGFTFTPKEAIEKRDEGLKILSK
                     HISQEDLFIIMENFSKSLGFKCRDCHVRSKTDPERLDYESYDNPHKKEALEMMKMTMK
                     INARDFKIKGDFTENFTKNKYMITCYSCHNGSPHPETELPDELLKQKVDELAY"
     gene            complement(1483..1857)
                     /gene="ssrA"
                     /locus_tag="PLG24_03175"
     tmRNA           complement(1483..1857)
                     /gene="ssrA"
                     /locus_tag="PLG24_03175"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1960..2733
                     /locus_tag="PLG24_03180"
     CDS             1960..2733
                     /locus_tag="PLG24_03180"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HMZ23918.1"
                     /translation="MMTKCYEISHLYLYPIKGLPGILLEKAEVKQGGLAKDRLFAIFD
                     GSGKMLTRRNIDGMADFEMGLYEDGVRVLSKRLKREIGITFNLDGIEASVHVWNRVVA
                     AKEVGLQPDLFFSEHFGKEVRLMCRGSETEVPGFHDNSPILIINSISVRELEIAHNVS
                     IDIRRFRPNIVVSGDRAFEEQGWNAIRLGTIGTEMIKQCSRCVIVNQNPETTLADMNV
                     LELLIPYGLKDNKIKFGWYVAPQSSGNVYRGQQITIDVK"
     gene            2819..3013
                     /gene="rpsU"
                     /locus_tag="PLG24_03185"
     CDS             2819..3013
                     /gene="rpsU"
                     /locus_tag="PLG24_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HMZ23919.1"
                     /translation="MLIIEVKDGETIDRALKKYKRKYEKSGTIKQLRKRKHFTKPSIL
                     RRQEILKAVYKQATYGDNAL"
     gene            3057..3956
                     /locus_tag="PLG24_03190"
     CDS             3057..3956
                     /locus_tag="PLG24_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HMZ23920.1"
                     /translation="MQDVLKRFYDYIVSVERYSDNTKQSYQNDLDGFASFLCITYQLP
                     TFHEITHFHIRSWIVSLMDSGHSVRTIHRKISSLSTFFKYLRRFEGLATDPLKKIVRP
                     KSMKRLPISIPSDDLITISSLELAETDDYQANLEEMVVLMLYSAGLRRSELIQLREED
                     IDRQRLMLKIVGKGKKERFVPIKRELLTKIDEFIGLKNKTINVVEKGPIFLTRTGKPM
                     YPKMVYNIVSRFLNNIPNLERRSPHILRHSFATHLSDAGADINAIKMLLGHSSLAATE
                     VYMHNAPQRLRSVYLKAHPRSEE"
     gene            3992..4294
                     /gene="raiA"
                     /locus_tag="PLG24_03195"
     CDS             3992..4294
                     /gene="raiA"
                     /locus_tag="PLG24_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HMZ23921.1"
                     /translation="MKVTIEADQFKADTSLTDFIEKKLSKLDTFYDRIIDAHVILKLE
                     NAGQVRDKVVDIILNVPGDTMVVKEIDKTFEASVDKGIDNLKRQLIKKKEKLRATH"
     gene            complement(4367..5053)
                     /locus_tag="PLG24_03200"
     CDS             complement(4367..5053)
                     /locus_tag="PLG24_03200"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ23922.1"
                     /translation="MKFFTFLLCFFLFSTVANAQTFKLAEDSMSTVVEKDEFENYIQN
                     FAFNLTTETKVLRWTQKTIFKGSQWSNSQVCDNNLCYTDKILSKTVEVPAGGNTLLKL
                     LFRPNGQDTCGYYTITIKEEGKDNDEGVAHYFFNGTDCEAVASGLTKISNVETVRIYP
                     NPAKDYVQLSSLEAVKNIAIYNTEGRLIKEIMDYRNGLIPVNDLNRGTYFMMLSFHNG
                     KLGVSKLFKD"
     gene            complement(5711..6448)
                     /locus_tag="PLG24_03205"
     CDS             complement(5711..6448)
                     /locus_tag="PLG24_03205"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HMZ23923.1"
                     /translation="MIIEIAANSLQSALNAQDGGASRIELCSALELGGITPGAGLIRK
                     VKHALQIPVFVLIRSRSGDFIYSDQEMEVMLEDIRFCVSEGIDGIVWGGLKPDGTIDE
                     ERLQMIKKEAKGMQITFHRAFDHCIDPIAGLNTLIANKIDRLLTSGQTSNVVEGRMLI
                     KKLIKEAKGRITIMPGCGVNENNIQPVAAFTGASEFHASMKTLVKSRTLFNNKNVLLS
                     SGADIPEYDYFVSDLVKVRKLVSASME"
     gene            complement(6576..7199)
                     /locus_tag="PLG24_03210"
     CDS             complement(6576..7199)
                     /locus_tag="PLG24_03210"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HMZ23924.1"
                     /translation="MAFTLPELSYEYDGLQPYIDAETMRIHHTKHHNAYINNLNAAIE
                     GTEFANMTIEEILENAGRAGAAIRNNGGGHYNHTLFWKLLSPNGTKPSGEALDGINKK
                     WGSFDGFKEAFSKAAATRFGSGWAWLVVNKDGSFDINSTANQDNPMMGKEVSGVEGFP
                     ILGLDVWEHAYYLNYQNRRPDYIEAFFNVIDWDEVNSRLVQSTEYFK"
     gene            complement(7279..8484)
                     /locus_tag="PLG24_03215"
     CDS             complement(7279..8484)
                     /locus_tag="PLG24_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HMZ23925.1"
                     /translation="MRFPIRKIINDPVYGMVKIPDGIIFRVFEHPYFQRLRRIGQAGL
                     TNLVYPGANHTRFHHALGAMHLAIEALDMLELRNVDISEDEATGLILAILLHDVGHGP
                     FSHVLEHNLTGQHHEVLTLAIMNKMNMELEGALDTAVQIFTDKHPKKFLHQLISGQLD
                     LDRMDYLNRDSFFTGVAEGKIGYDRIIKMITVVDNNLVIQEKGIYSVENFLMARRLMY
                     WQVYLHKTVLAAEHMLIQCLRQARESVEKGEKFNISPHFLYFMQQKSFINEDEMLGHF
                     LKLDDYDLWWAIKQFAESRDQSLAFLADAILNRKIFKIELSKTPYSDEKITELVQQVG
                     NTFHLDEKHAAALVFHGSETNEMYNSKKDEIMVLMKDNSVRPFSEVSDYPVDKTEVVK
                     FFLCYPKLS"
     gene            8849..10408
                     /locus_tag="PLG24_03220"
     CDS             8849..10408
                     /locus_tag="PLG24_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HMZ23926.1"
                     /translation="MEKIKILWVDDEIETLKPQLMFIQQKGYEVTAVTNGYDAMEEFE
                     SDNEFDAVFLDESMPGISGLETLSRIKAIKPNIPVVMITKNEEEHIMEEAIGAQIADY
                     LIKPVNPNQILLSLKRIVDEKRLIREKTSSDYQQDFRQIFMKINSGLDHQEWVDVYKK
                     IINWEIKMDQSQSLEMGDILSSQKAEANTAFCKFVDRDYMEWLDKDNKEAPVMSYNLL
                     REKVFPWVNDEMPTVVVLMDNLRYDQWKVIEPIINECYKVESEDYFFSILPTSTQYSR
                     NAIFAGMMPSEIEKKYPDLWKNDVDEGGKNLSENVFLADQITRVFRKPIKHEYQKITN
                     IQNGKALADNALNFLNNNLSIIVYNFIDMLSHARTEMEVLKELADDEKAYRSLTKSWF
                     IHSPLWLALQRLAEKPIRLFILTDHGSIKVKSPSKVVGDRETTTNLRYKVGRNLNYVG
                     NEVIDFKDPSKVYLPRPNVSSSFIFAKEDKFFLYPNNYNYYNNYYKDTFQHGGASLEE
                     IICPVIRLKSR"
     gene            complement(10423..>11156)
                     /locus_tag="PLG24_03225"
     CDS             complement(10423..>11156)
                     /locus_tag="PLG24_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HMZ23927.1"
                     /translation="LMGTFHVFVPEFSPLIEKAEKLISKCSHYYGEMDLTQVDPLIIN
                     ETMQSLPRPGKEVLYLLENQQPYFEKMGLPFPAYPAQNSIFNLYSELTMSMWRKKILV
                     GSLDEELIKIAMKNHIPIGGIESFNDQLEIMKSIDYEKMEKQIIKMLIKPKSFSKNMD
                     HLLDFYLKGKMNVLSKKSKGSSRGNRKLMVKDRNRVFADTIARICEESDRNFISFGAG
                     HLGGKDGVIRRLKKAGFKVRKVSLD"
BASE COUNT         3436 a         1966 c         2296 g         3458 t
ORIGIN      
        1 ggaaggaaat tgatatttca aatgccggaa tattttttct ttttctagct tccggcatta
       61 ttctctccag ggttacttca ggtaagattg tagataaagg ttatatccat attgttatga
      121 tggtagcaat tgttacgatt tccgtaggat tcacttgttt tgcaatgctc cataatatat
      181 ttgcatatgc aagctcggcc tttgtgctgg gtatcggata tggcacctta ttccccgcct
      241 tacagaccat ctatattgat atggcaccag cctccaggcg tggaacagcc aattcgactt
      301 atttgttggg ctttgacctg ggaataggga ttgggatgct tcttggtgcc atacttgaag
      361 tcaggtatgg ttttcagagt atgtacctta tgacggcagg cttttgtatt ctttcactta
      421 ttatgtattg gacggtatcc agaaagattt ttgagcggta taagttggaa aaagattaag
      481 ggggaatttc aggaaacact attatgcaag agtaaaaaag ggtattgcca gggaagtagg
      541 tagatgatta ataatcaact tgccttatgg catgcatgag tacatggctg tgtattcatt
      601 acaaggccac ttatagtggc tatatttata aattattttt caaaagccct tatactccag
      661 cgtttgagat tattaacttt ctacctatct ggatttgatg cctccataat aatcaagaaa
      721 agcaatctcc aaaaccgttt taattcaacg agtagtccag gtgggatgta tttgataagg
      781 atggcgaata tagctcgcaa ataatcagta ggccaattcg tccacctttt gtttcagcag
      841 ttcatcaggg agttcagttt cgggatgagg tgaaccattg tggcatgaat aacaggtaat
      901 catatatttg ttttttgtaa aattttcagt aaaatcgcct ttgattttaa aatcccgggc
      961 attgattttc atagtcattt tcatcatctc gagtgcttct tttttgtgcg gattatcgta
     1021 agattcataa tccaatcttt caggatctgt ctttgatcgg acatggcaat cccgacattt
     1081 gaatcccaga gatttactga aattttccat aatgataaaa agatcttcct gtgagatgtg
     1141 ttttgaaaga attttcagcc cttcatcccg tttttcaatt gcctcctttg gcgtaaaggt
     1201 gaatccactg aataaaatca tggctgatgc aagtatggtg ccaataaatt tcttgttaac
     1261 cggaaatgcc atatcgaata gaattaatat tagatgaaaa aatagactat aattagcaac
     1321 aggagaccga catctactta cctcgtagca taattgccgt caaaagataa agttaatact
     1381 tctttttgaa aaaatgaaat tgtaagtcca aaatagcaac gataggatca aagtcaaggc
     1441 agtgataaaa aatactatag tgtaaattga aataagaaaa atatgtggag ctggagggat
     1501 tcgaaccctc gtccagagaa agcgcctgaa agctttctac atgcttagtt tttctttaaa
     1561 ttttcgagtg aggataagtg gaaaaacaca ccacatccta accttagctc cttagatctc
     1621 ggaaacacct cggagcggaa tatttccata cctgaattat tgatgacatg cgacattacc
     1681 ttatatcagg tgtcaggtaa attgcatgaa cgcgtgttaa tctaccagat taagctgcga
     1741 ttgcgaaatt agtttcgcca attattgttc aatagcactt tggtgaagaa ggtagctatc
     1801 atactcctgc atgcttacta accgatcata actctcctgt cgattccagt cagccccata
     1861 tttttcaaag aactaacgcg tataacggta tagaggcatt aaaagttcac atatttaaaa
     1921 aaatatttga aaattgtaga ttcttccgat ttttactcta tgatgacaaa atgctacgaa
     1981 atttcacatt tatacctgta tccgataaag ggccttccgg gaatactttt agagaaagcg
     2041 gaggtcaaac agggaggttt ggccaaagac agattgtttg caatttttga tggatctggc
     2101 aaaatgctta caaggcgaaa tattgatgga atggctgact ttgagatggg tttatatgaa
     2161 gatggggtca gagtcttatc aaaaagactt aaaagggaaa ttggcatcac atttaattta
     2221 gatggcattg aggcttccgt ccatgtttgg aatagagtgg tggctgctaa agaggtcggt
     2281 ctgcaaccgg atttgttttt ctcggagcac tttggaaaag aagttcgatt aatgtgcagg
     2341 ggatcagaaa ccgaagtccc tggattccat gacaattcac cgattcttat catcaatagc
     2401 atttctgttc gggagttgga gattgctcat aatgtttcta ttgatatccg taggttcagg
     2461 ccaaacattg tggtttccgg ggacagagca ttcgaggagc aggggtggaa tgcaatacgt
     2521 ttgggaacaa ttggaacaga gatgattaag caatgtagca gatgtgtcat tgtcaatcaa
     2581 aatccagaaa caactttggc agatatgaat gtcctggagt tgttgatacc ttatgggctg
     2641 aaagataata agatcaaatt tggctggtat gtggcaccgc aaagttccgg aaatgtatat
     2701 cgtggccagc aaattactat tgatgtaaaa taataatata aaaaataata atgaattccg
     2761 gaaaaagtgt acctttgcag ctctaattta taaaaaacaa aaaagaagtt ttcattttat
     2821 gctgattatt gaagtaaaag acggtgaaac cattgacaga gcacttaaga agtacaagag
     2881 gaaatatgag aaatcaggta ctatcaagca gttgcgtaaa cgtaagcatt tcaccaaacc
     2941 gtctatcctc agaaggcagg aaatcctgaa ggctgtatat aagcaggcca catacggcga
     3001 taatgccctg taaaaaatat tgatattatt tacattttgc atatattcac acttatatgc
     3061 aagatgtact taaaagattt tatgattata tcgtttctgt agaacgatat agtgacaata
     3121 caaagcaaag ctatcagaac gatcttgatg gctttgcttc ttttttatgt attacttatc
     3181 agcttcctac atttcatgaa atcactcatt ttcacataag atcctggata gttagtctga
     3241 tggattccgg gcattctgtt aggaccattc atcgtaaaat ttcttcactg agtacatttt
     3301 tcaagtattt acgccgtttt gagggtttgg caacggatcc ccttaaaaag atagtaaggc
     3361 ccaagagcat gaaaagattg ccgataagta ttccaagtga cgatttgata acaatttctt
     3421 ccctggaatt ggctgagaca gatgactatc aggctaacct ggaagaaatg gtagtgctga
     3481 tgctttatag tgccggtttg cgaaggtcag agcttatcca attgcgggaa gaagatatcg
     3541 acaggcaaag actgatgctt aaaatagtag gaaaggggaa gaaagagcgt tttgttccaa
     3601 taaaaagaga attacttact aaaattgatg aatttatcgg gcttaaaaat aagactatca
     3661 atgtagtaga aaaaggtcca atatttttaa cccgaaccgg gaaaccaatg tacccaaaaa
     3721 tggtttacaa tatagtaagt cgttttctaa ataatattcc taatttggag cgaagaagcc
     3781 cacatatttt aagacactcc tttgctactc atctctctga tgccggagcg gatatcaatg
     3841 ctataaagat gttactaggc cacagtagtc ttgcggctac agaagtatat atgcataatg
     3901 cgccacagag gcttagatcg gtatatctta aggctcaccc gcgatctgaa gaatgacgat
     3961 aaaatattgt ttaacataaa aatgtaagca gatgaaagtt actattgaag cggatcagtt
     4021 taaggctgac acaagtttga cagactttat tgaaaagaag ttatcaaaac tcgacacatt
     4081 ttatgacaga attattgatg ctcatgtgat tctcaaattg gaaaatgccg gtcaggtaag
     4141 ggataaggta gtagatatta tccttaacgt gccgggagat accatggtcg ttaaggaaat
     4201 tgacaaaacc tttgaggctt ctgttgataa aggtatagac aatctcaaac gacaattgat
     4261 taagaaaaag gagaagttga gggctactca ctaacatata aagaaaaagg ccgtgcaaac
     4321 ggcctttttt tattttatca agtaatttat tgcaggttgg ttgcaatcag tctttaaaca
     4381 gctttgatac acccaatttg ccattatgga atgaaagcat cataaagtaa gtcccccggt
     4441 taagatcatt tacaggaatt agtccattac gataatccat aatttccttg atcagtcggc
     4501 cttccgtatt atagatggca atattcttaa cggcttcaag agaactaagt tgaacataat
     4561 ctttggcagg atttgggtat attctgacgg tctctacgtt ggatatttta gttaggccgg
     4621 atgcaactgc ttcgcagtct gtcccattga aaaagtaatg ggcaacacct tcatcattat
     4681 ccttaccttc ctccttgatt gttatcgtat aataaccaca tgtatcctga ccattcggtc
     4741 tgaacagtaa ttttaatagt gtattgccac ctgccggtac ttcaacagtt ttagaaagga
     4801 tcttatccgt gtagcagaga ttattgtcac aaacctgtga gttactccat tgtgatcctt
     4861 taaatattgt tttctgtgtc cagcgcagta ctttagtctc agttgtcagg ttgaacgcaa
     4921 aattctgaat gtagttttcg aattcatctt tttcgacaac tgtggacatt gagtcttcag
     4981 ctaatttaaa ggtctgagca tttgcaactg tgctgaagag gaaaaagcaa agtaaaaaag
     5041 taaaaaattt catgacagaa ttttaggaga aaataaaatg atatttggcg gtgcaaagtt
     5101 acttatttgt tactattttg aataaatatt taatgacata ttatttaata aaatgcaaaa
     5161 ggggcccgat taacatgagc agaaaaaata tagccgggaa taaatgaagg agataatttg
     5221 catagcaaat tgtatgatga cgtggtaatt gatttcacgt caggtttcag gaatgaatag
     5281 caataccgaa ggtacattcg ttatagacca atttcattga tctatacgac tttaacctcg
     5341 gcatcccgga accccggatt gtttcactcc cgggacagag cagagctcta gtagcatagc
     5401 tatccggaca agtataccca aaatttatgg actattatat aaatcatctt ggtagaacat
     5461 gttataaata atatattgac aatcaggaca atgaggttgt gaagagggca ggggcttaga
     5521 aaacaatgta tcttttggga ataatagtca attcagatac ttcggtgaga actctaatat
     5581 agataaaatt tcagtctata aatgtcctat tgaactaatt tttctgtttt tttggaaact
     5641 tcctccacca acattagcat ttgcgaaatt gcatagtagg tagacagctt ggtattatgg
     5701 agaacggttg tcattccatt gaagcagata ccaattttct cactttaacc aaatcggaga
     5761 cgaaataatc atattcagga atgtcggcac cagagctcaa caatacattc ttattattga
     5821 acagtgtccg gcttttgacc agggttttca ttgatgcatg aaattctgag gctccggtaa
     5881 aagcggcaac aggttggatg ttattttcat tgactccgca acccggcata atagtgatgc
     5941 gaccctttgc ctctttgatt agttttttga tcaacattct gccctcgact acattactgg
     6001 tctgcccgga ggtcaatagt ctgtcaatct tattcgctat cagggtattt aagcccgcaa
     6061 ttggatcgat acaatgatcg aaggctcgat ggaatgtgat ttgcattcct tttgcttctt
     6121 ttttaatcat ctgtagtctt tcctcatcga tggtgccgtc gggtttaagt ccaccccaga
     6181 caattccatc aatgccttca ctgacgcaga atctgatatc ctccagcata acctccattt
     6241 cctgatctga ataaataaaa tctccgctcc ttgagcgaat tagtacaaat acaggaattt
     6301 gaagagcatg ttttaccttt cttataaggc cagcacccgg agtaatgcca cctaattcaa
     6361 gagcagaaca taattctatt cttgaagcgc ctccatcctg ggcgtttagg gctgactgaa
     6421 ggctattggc ggctatttcg atgatcatgt agaggtaaaa attgaaaggt aaatatactt
     6481 cctaaaaata aatcagctgc aaattacagc tgatttattt catgtcgact ttttgttatt
     6541 gtattgaaat gtgagttgtc tcaatagacg gaccattatt tgaaatattc ggttgattgg
     6601 actaggcggc tattgacttc atcccaatca ataacattga agaatgcctc aatatagtcg
     6661 ggtcttctgt tctggtaatt tagataatag gcgtgttccc aaacatcgag gcccaggatc
     6721 ggaaagcctt caactccgct tacttctttg cccatcattg gattgtcctg gttggcagtg
     6781 gagttgatat cgaaagaacc atctttattt acaacaagcc atgcccatcc ggacccaaat
     6841 cgggtagcag ccgctttaga gaaagcctcc ttgaaaccat caaatgatcc ccattttttg
     6901 ttgataccat ccaatgcttc tcctgaaggt ttagtaccat ttggagaaag caacttccaa
     6961 aacaaggtgt gattgtaatg accgccgccg ttgtttctga tagcagcgcc ggctctacct
     7021 gcattttcaa ggatttcctc aatagtcatg ttggcaaatt cagtaccttc tattgctgca
     7081 ttgagattat tgatataggc attgtggtgt ttggtgtggt gaatcctcat cgtctcagca
     7141 tcgatatacg gttggagtcc atcatattca taactcaatt caggaagtgt aaaagccatt
     7201 gtataattat ttaaagttaa aaaaatattt gaccgggtta aaatttgcaa cgtaataaac
     7261 taaacaactt aaaagtattc aagaaagttt agggtagcat aagaaaaact ttacaacttc
     7321 tgttttatca accggataat cggacacttc agaaaatggc ctgactgaat tatccttcat
     7381 aagaaccatg atttcgtcct ttttgctatt gtacatttca ttggtttcag aaccatggaa
     7441 gacgagagca gcggcatgtt tttcatccag atgaaatgta ttccctactt gttgaactaa
     7501 ttcggttatt ttttcatctg aatagggtgt tttactcaat tctatcttga aaatttttct
     7561 gttgagaatg gcatctgcta aaaaagcgag ggattggtcc cgactttcag caaattgctt
     7621 gatcgcccac cacaagtcat agtcatccag tttcaggaaa tgtccgagca tttcatcttc
     7681 atttatgaag gacttttgct gcataaaata tagaaagtga ggagaaatat tgaatttttc
     7741 gcccttctcc acgctttcac gagcctggcg taggcactgg atgagcatat gttctgctgc
     7801 aaggactgtc ttgtgcagat atacctgcca atacataagc cggcgtgcca tcaggaaatt
     7861 ttcaacggag tagatgcctt tttcttggat aacgagatta ttgtctacaa cagttatcat
     7921 tttgatgatt ctgtcatatc ctatttttcc ttctgctact ccggtaaaga aggaatcccg
     7981 gttgaggtaa tccatcctgt ccaggtctag ttgtccggaa atcaattggt gaagaaattt
     8041 cttaggatgc ttatctgtaa aaatctgtac tgccgtatcc aatgcacctt ccaactccat
     8101 gttcatttta ttcattattg ctaatgttag cacttcatga tgctgtcctg tgaggttgtg
     8161 ttccaataca tgactaaatg gtccatgtcc aacatcatgg agtaaaatgg cgagtatgag
     8221 cccggtagct tcatcttctg aaatatcaac attcctaagt tccagcatgt ccagggcctc
     8281 aatggcaagg tgcatggcac ctaatgcatg atggaatctg gtatgattgg cgcccggata
     8341 tacgaggttt gtcaggcctg cctgtccaat tcgtctaaga cgctggaaat atggatgttc
     8401 aaaaacccga aaaatgatgc catccgggat tttaaccatg ccatatacgg gatcgttaat
     8461 aatttttctt attggaaaac gcatgcatta gattgtttgt gccataaagg taattgtatg
     8521 atgtcttaaa ttttgctttg aatgggaaaa taatgtggat aattaaaata ggaatcggta
     8581 aatttttcct tttagattga taagaggcat ttgaaagccg gaattatgtt ggtaaagagt
     8641 agtttataac tcttcgtcct gctcttttca aataatgagt ttaatcgaat attggttttc
     8701 cggatctggc agaaagtaaa aataccttat agaatatctc cagactttga aaaaataatg
     8761 tatccatcaa ataatgaacg aatcttgtcg tttatttgta ttatgaaata tagtttaaaa
     8821 tttttcattc aacgaataat ataaatagat ggagaaaata aagattttgt gggttgatga
     8881 tgagatagag acattaaagc ctcagttaat gtttattcaa cagaaagggt atgaagtaac
     8941 ggctgtgacc aatggatatg atgcaatgga ggagtttgag agtgacaatg agtttgatgc
     9001 tgtatttctg gatgagagta tgccgggtat ttccggcttg gagaccttgt cgagaataaa
     9061 ggcaatcaag cccaatattc cggtcgtaat gatcactaaa aatgaggaag agcatatcat
     9121 ggaggaggct ataggagcgc aaattgccga ttatctgata aagccggtca atcccaatca
     9181 aattctattg tctctgaaaa gaatcgtaga tgaaaagcgt ctgataagag aaaagacttc
     9241 cagtgactat caacaggatt ttcgccagat atttatgaag atcaatagcg gtctcgatca
     9301 tcaagaatgg gtggatgtat ataagaagat catcaattgg gaaatcaaaa tggatcagag
     9361 tcaatcgctt gagatgggtg atatactgag ttctcagaaa gctgaagcca atacggcatt
     9421 ttgcaaattt gtggatcggg attacatgga atggttggat aaggataata aagaagcgcc
     9481 tgttatgtcg tataatttgc tcagggaaaa agtcttccct tgggtgaatg acgagatgcc
     9541 gactgtagtg gtgttgatgg ataatctaag atatgatcag tggaaggtta tagagcctat
     9601 aatcaatgag tgttataagg tagaatctga agattatttt ttcagcattt tgcctacttc
     9661 aacacaatat agcaggaatg cgatttttgc gggaatgatg ccaagtgaga tcgagaaaaa
     9721 gtatccagac ttatggaaaa acgacgttga tgagggaggc aagaatcttt cggagaacgt
     9781 ttttttagct gatcaaatca ccagggtatt tcgtaagcct ataaagcatg aataccagaa
     9841 gattacaaat attcagaatg gaaaggcttt ggctgataac gcgctcaact ttctcaacaa
     9901 taacctgagt ataattgtat ataactttat cgatatgttg tctcacgccc ggacagagat
     9961 ggaggtattg aaagagctgg cagatgatga aaaggcttat cgttcactca ccaagtcctg
    10021 gtttatccat tctccgcttt ggcttgctct tcaacgtttg gccgagaagc caattcgatt
    10081 gtttattctg acagaccacg gctccatcaa ggtcaaatct ccttctaagg tggtcggtga
    10141 ccgggagaca accaccaatc taaggtataa agtcggaagg aaccttaatt atgttgggaa
    10201 cgaggtaata gattttaaag atccttccaa agtttatcta ccgaggccaa atgtaagttc
    10261 gtcattcatt tttgccaaag aggacaagtt tttcctgtat cctaataatt acaactatta
    10321 taataattat tataaggaca cttttcagca tggaggagca tctcttgagg aaataatatg
    10381 tccggtgatc agattgaaat caaggtaaca ggtaataagt gattaatcaa gactgacttt
    10441 tctgacttta aaaccggctt tttttagtct gcggatgact ccatcctttc cgccgaggtg
    10501 accggcgccg aaggaaatga aatttcggtc tgattcttca caaatgcggg caattgtatc
    10561 agcaaagact ctgttacggt cctttaccat cagttttctg ttacctcttg aggagccttt
    10621 agatttttta ctcagaacat tcatttttcc tttcagatag aagtcaagaa ggtgatccat
    10681 gtttttggaa aatgatttgg gtttaatcag cattttgatg atctgttttt ccattttttc
    10741 ataatcaatt gatttcatga tttccaattg atcattgaag ctttctattc ccccaattgg
    10801 gatatgattt ttcatggcaa ttttaataag ttcctcatca aggcttccga caagaatttt
    10861 ttttcgccac atactcatag ttagttcaga gtataggttg aatatggagt tttgtgccgg
    10921 ataggccggg aagggtagtc ccattttttc aaaatagggt tgttgattct caagtaaata
    10981 aagaacctcc ttgcctggtc tgggcaagct ttgcatggtt tcattgatga ttagcgggtc
    11041 cacttgagtc aaatccatct ctccataata atgagagcac ttggagataa gtttttcggc
    11101 tttttcaatc aaaggactga attcaggcac aaaaacatga aaggtaccca tcaaat
//
LOCUS       DAOQQN010000052        11069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2117_length_11069_cov_5.736880, whole genome shotgun sequence.
ACCESSION   DAOQQN010000052 DAOQQN010000000
VERSION     DAOQQN010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11069
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2117_length_11069_cov_5.736880"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2282
                     /locus_tag="PLG24_03230"
     CDS             <1..2282
                     /locus_tag="PLG24_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ23928.1"
                     /translation="NSNVGTNISGNTWDFTGVTLGNYSFTFVPTGAQAPCTNDPTTII
                     VKVTDECDCPTLQVITPTTALCNDALSIVDLNTMINSSEAGVWTLVKDPDGVKNTLIP
                     NGKFDPTGKTLGTYDFQFEINITPPEDCDSLLTVSVTLNPAVIFQLQPTAEVCNFDPK
                     NQNDNQLDFGSSTIWASNPVAGTWEDTDNSGAVKGSGNIWTFNGVPTGVYTFTFTPSG
                     ALPPCQNIPQTIEITVVDNCDCEDLPALAPIGAVCNNQSVINLIAPAGYTGVWSIKSK
                     PAGSNPVVINGSSIDIGGKDEGTYTLRFKFDNQPSDCKDTAFVNFVLYDYKSAGLASP
                     AQQYCEKTKVIIDLQDELTGEDPGGLWTEVGPSSAGSALTGTILNIEKLLPGHYIFQY
                     KHVTNGACPEQISEIDITVNPNPKADAGSSFQLTCEQASTIIGGIGSSTGSDIAYQWT
                     EKNNNPIPNPKELFNEVKLEGIYYLKVTNTNTGCTAFDSVEVTSDPNRPQGIVIERNN
                     PTCYGYKDGYIKVAKIEGGAPPILYSLNDGPFSSINSWTNLSGSNNTLKIKDANGCLL
                     VLDNIILVEPTLVTVEMGNDTLINLGDTVLIDPDISIPHDSISSIIFSSDYDFINCTG
                     CFDITVFPNQTTTYSVEVKDKNGCSDKDSKRVVVRKGVNIFVPNIFTPNGDGNNDKVF
                     ISTNDREIKQINSFQIYDRWGEKVYEALNYQPNDPSTGWDGTLKGQKCNPAVYVYWAE
                     IELFDGTKQIIKGDVTLMR"
     gene            2682..8990
                     /locus_tag="PLG24_03235"
     CDS             2682..8990
                     /locus_tag="PLG24_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ23929.1"
                     /translation="MKIVIHALLATCIITLFTQWSWAQPPANDDCANAVDITLLDGTC
                     QVFQFDTCTYDLANGSCVPGANPNIWFSFTAQGPQANILVKPLGNKVTATLAYFPNGC
                     ANTTGAQEFGCGADFNVSSLTPGTTYFVIVNVLILPLDSIEICINNPVPPPNDDPCTA
                     QVIPQNGCVNGTTVGADPDYQNPGCPTQSENAVFYSYTLGPNTDQLDISIDNNGITGN
                     MGIALLDFPNGCASPPTLAANGSFYCGPPINTYSFQNLTPGSTIYIMITSTTAGAGDF
                     TNMCLTEQEGQPPCNTNGDCGTAIAIDIPNSAEPVCVTGCNVGMPDGPILNGGGACTN
                     MDNPVAWYSFNSGNNNTAVINFSSGDLTSPIYALFNSCNTWVECNPVSASVIPNTTYY
                     IAVTDADGAEGNFELCITLLNITAPCITSQSLTIVGANMGSPLTGPFKKCEEVTFKYT
                     TNFIKIGSQWIHSMFPVISNCFDYQQGTEPTPSNRPTGSATWDWYPANTVHWKSLDNS
                     SGAVGINLGTGNICIIGTPGCQAFVGGGNCSWTGTSMPAGWIGPTYSGTCSSSIPNLS
                     YGDPASGPFSVQFTVKIPCSACTDQSCNDYTVAITAFSDGQTGGWQSSSCNGHTLLTK
                     KITVQCCTEPTMTLQDGQICSGETFFANITLDPPNSTLQWTVVNANGVSGATSGSGST
                     FSQTLTNNTGSSKTVIYSVTPISPTGCVGQPQTLNVLVFPQVIANAGPDKTSCPGATV
                     TLGGNPTASGGAGAPYTIAWSTGENTENIVVSPTINTTYSVTVTDANGCSAVDQVIVS
                     VTGNLEVNILPNPAEYCLNELTGKTLSSAVNSQNGPFTYEWNTPWGQFTTATIPINAT
                     QAGSFQVTLQVTDKYGCKGIGTLTIKINPTPKINFLNKPDQPLCPLEAFILLTDPSSS
                     QGVVYSSHPDGFILPDGTILVDQMTPNIPYWIVAAYSDPVTGCKSRDSIQVSVLQIDD
                     PSITPAGPFCASENGPYQLIGTPAGGLWTGGVSSDGLFFPSALGPGIYTIEYTVGTGT
                     CTKQTFTTIEVKPNPTPYILNLSTYCNTDSPNPILITDIPGGTWSYPITPLGEIPINS
                     ISPGIYAISYSVTIDGCTGNVDDVLIINKQPEATIINTAVVCNADPGNDGKSKLNLND
                     FFTGGDASGSWTEIPPLSGAVNLGGNNYDFTGLPDGTIVRFEYIVPASLPCNDSRDTL
                     TVTVDDDCDCPKLLFSKPGDLCNDNASVDINTFIINAGSGGWALYSVPSGGNPGTLNG
                     TIFDATGKDPGNYIIRYTLNPEPTNAICPKFIDLTITVNPDVVYTMKPEIKVCNNVPL
                     PPSPKTMVNLDDFFTSNPVPGTWINNDNIGTNVIGNTWDFTGVPIGSYSFTFTSSNAV
                     APCQNKSLTVKVTVTENCNCPELSVLTPITTLCSDNQNTIDLNSLIVSAEAGKWALVN
                     DPTGQVSIPVANGFFDPAGKADGVYTFEFEIDITPPENCDSTVTVDIVIVPQLVYKLQ
                     DNATVCNKDPKSENADQLDFNSSVIWQSTPVSGIWVDTDGSGAIQTGNIWNFNNVPVG
                     TYIFTFVPSGAPSPCVNIPQTITINVVDNCECPDLPVFAPIPATCNNESEINLSIPNG
                     FTGTWSIKSSPAGSNPLSISGNTVNINKKDAGTYVLRFIVDNVKPGCPDSVFVNIILD
                     DFKFAGTEEALVTFCEKTSTIIDLADHITGEDSGGVWTEVGPSTAGSALIGSILKIKD
                     LKAGDYIFQYRFANGGSCPASSSEVKVTIFENPIADAGQDQELTCEISQATIGNPSSP
                     GNNLLYQWAEVSNKPIPDPNASSMVVTIDGTYILTVTDPKTGCSDKDTVVITSDPERP
                     KGIEIEREGPTCYGYKNGYVKISKIEGGAPPILYSFNGGPYSSINSWSNLSGSTNIIK
                     IKDANGCELTLDNIVLIEPPLVTVELGNDTLINIGDTVIIKPDISIPEDQVGSITYSS
                     DYDFINCIGCFHITVYPNQTTVYTVEVKDKKGCSDKDSKKIVVKKGVNIFIPNIFTPN
                     GDGNNDKVFISTNDKEIKRILSFQIYDRWGEKVYEAKDYQPNDPNNGWDGTLKGQKCN
                     PGVFAYWTEIELFDGTRQILKGDVTLLR"
     gene            complement(9044..9364)
                     /locus_tag="PLG24_03240"
     CDS             complement(9044..9364)
                     /locus_tag="PLG24_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HMZ23930.1"
                     /translation="MKDEIRITIIDREGQEHIYDIPPDIGISVMELCKANDLPILATC
                     GGMALCATCHVLVESSTTLPEMGADEEIMLDQLPNMENNSRLSCQLKITEQLDGLIVR
                     LAEE"
     gene            9756..>11069
                     /gene="pgi"
                     /locus_tag="PLG24_03245"
     CDS             9756..>11069
                     /gene="pgi"
                     /locus_tag="PLG24_03245"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HMZ23931.1"
                     /translation="MPLFKINPANTSSWKSLSSSQKRLEGVDIKTLFSADENRFDNYS
                     ISFGDIMFDYSKNRIDGQVMAELLGLAEECKVRESIDAMFGGEKINETEGRSVLHIAL
                     RNRSDRPILSNGNDVMPEIRRVLSKMKTFCHKIHSGAHKGFTGKKIKHIVNIGIGGSD
                     LGPVMVTEALRPYWLEGIQAHFVSNIDGTHIAEVLKVINPETTLFLIASKTFTTQETM
                     TNAATARDWFLSKGGSSQDIALHFAALSTNEKAVEAFGINTENMFEFWDWVGGRYSLW
                     SAIGLSIALTIGFDHFEQLLEGGYSVDEHFKNTVFEKNIPVIMSLIGIWNCNFLGAHT
                     EAILPYDQYMHRFPAYFQQGNMESNGKSVARDGNEVTVETGPIIWGEPGTNGQHAFYQ
                     LIHQGTRLIPCDFIAPAISQNPLPGHHDKLLSNFFAQTQALAFGKS"
BASE COUNT         3387 a         2353 c         2199 g         3130 t
ORIGIN      
        1 ataactctaa tgttggaact aatataagtg gtaacacctg ggattttaca ggagtaacat
       61 tgggtaatta ttccttcacc ttcgtaccaa ccggtgcaca agctccatgt actaacgatc
      121 ctactacaat tatcgtaaaa gtaaccgatg agtgtgactg ccctaccctt caggtaatta
      181 ccccaactac agcactttgt aatgatgctc ttagtattgt tgacttaaat actatgatta
      241 attcatccga ggctggagtg tggactttag tgaaagatcc ggatggtgtt aaaaacacat
      301 tgattccaaa tggcaaattt gatccgacag gtaaaacact tggtacatac gacttccaat
      361 tcgaaatcaa cattacccct cctgaagatt gcgacagttt attgactgta tctgtcaccc
      421 ttaatccggc tgtaatcttc caattgcagc cgactgcaga ggtgtgtaat ttcgatccaa
      481 aaaaccagaa tgataatcaa ttggattttg gtagctcaac tatttgggcg agcaatccag
      541 tagccggtac ttgggaagac actgacaact caggtgcagt gaagggctca ggtaatatct
      601 ggacattcaa tggagtacct actggagtat atacttttac atttactcct tcaggggctt
      661 tacctccttg tcaaaatatt ccgcaaacta tagaaatcac agtggtagat aactgcgact
      721 gtgaagacct gcctgcgctt gccccaattg gagcagtatg caataatcaa agtgttatca
      781 acttaatcgc accggcaggt tataccggag tctggtcaat aaaatccaaa ccggccggtt
      841 caaaccctgt tgtaattaat ggatcatcaa ttgatattgg aggcaaggac gaaggaacat
      901 atacactaag atttaaattt gacaaccaac cttcagattg taaggatact gcttttgtta
      961 acttcgtctt atatgactac aaaagcgctg gactagcaag cccggcacaa cagtattgtg
     1021 agaaaactaa agttattatc gacctgcaag acgaactgac cggagaagat cctggtgggc
     1081 tttggaccga agttggacct agcagcgcag gatcagcact gactggtaca atccttaaca
     1141 ttgaaaaact gctcccgggt cattatattt tccaatacaa acatgtgaca aatggagcat
     1201 gtcccgagca gatttcagag attgatataa cggtcaatcc aaatccgaaa gctgatgcag
     1261 gtagctcatt ccaattgacc tgtgaacagg cctccaccat aatcggagga attggaagta
     1321 gtaccggatc cgatatagcc taccaatgga cggaaaaaaa caacaatcca attcctaatc
     1381 ccaaagagct gtttaacgaa gtaaagctgg aaggcatcta ttacttaaaa gtaaccaata
     1441 ccaatactgg ttgtacagca tttgactcag tggaagtaac cagtgatcca aacagaccac
     1501 aaggcattgt tattgaacgc aataatccta cttgttatgg gtacaaagat ggatatatca
     1561 aagtggcaaa aattgaaggg ggcgcacctc caatcctata ctcgcttaat gacggtccat
     1621 tctcctccat caattcctgg actaatcttt caggtagcaa caataccctg aaaatcaaag
     1681 atgctaatgg ctgtctcttg gttctggata atataatatt agtcgaacca acactggtta
     1741 cggtagaaat ggggaatgat acattaatca atctgggaga taccgtgcta atcgatcctg
     1801 atatttccat tcctcacgac tcgattagca gcatcatatt ctcatcagac tatgatttta
     1861 ttaattgcac gggatgtttc gatattacag tattccccaa ccaaaccaca acatactctg
     1921 tggaagttaa ggataaaaac ggatgtagtg ataaggacag taaacgtgta gtggtacgta
     1981 aaggcgtcaa tatatttgtt cccaacatat tcaccccaaa tggagatggt aataacgaca
     2041 aggtgttcat ttccacaaat gatcgggaaa tcaaacagat taactcgttc caaatttatg
     2101 acagatgggg agaaaaagta tatgaggcac taaattatca gccaaacgat ccatccaccg
     2161 gttgggatgg aacacttaaa ggccaaaaat gcaaccctgc ggtttatgtc tattgggctg
     2221 aaattgaatt gtttgacgga accaagcaaa tcatcaaagg cgatgttacg ttgatgagat
     2281 aaatacaagt gaataaaaga tttttatatg gccattcagc attagaatgg ccatttttcg
     2341 tcaaataact ttcaaatttc tatccaaata gattgcaaac attgataata gtccataatt
     2401 atatttggaa gcatttttgc tctataataa gaatcttcta agcttacaca tcctttagca
     2461 tgctattcaa caccttgacc ttaattatga tttatttcta aaattttaat caaagtttgc
     2521 aataacgttg attcgaaata atcttctatg aatgaaagga atatactagt tacacaaaac
     2581 catatatggc aacattcaaa atatacgccg atatattttg atatactata aatttgtggt
     2641 ataaaatatt taatgtaatt ttgcagttca agaattaata catgaaaata gttatacatg
     2701 cccttcttgc aacgtgcata attacccttt ttacacaatg gtcatgggct caacctcccg
     2761 ctaatgatga ctgtgccaat gctgtcgaca taacattatt ggatggtaca tgtcaggtct
     2821 ttcaatttga cacatgtaca tacgatttgg caaatggatc ctgtgtaccc ggagctaatc
     2881 cgaacatatg gttttccttt accgctcagg ggcctcaggc aaatatttta gtcaagcctc
     2941 ttggaaataa agtcactgca acacttgctt actttcctaa tggatgtgcg aatacaaccg
     3001 gagcccagga atttgggtgt ggagccgatt tcaatgtttc ttccctgaca cctggtacaa
     3061 catattttgt tatagtcaat gtcctgattc tacctctgga ctccattgaa atatgtatca
     3121 acaatcccgt tcctccccca aatgacgacc cctgtacggc acaggttata cctcaaaatg
     3181 ggtgtgtgaa tggcacaaca gtaggtgcag atcctgatta tcaaaatccg ggatgtccta
     3241 cacaaagtga aaatgctgta ttttattcct atactttagg accaaatact gatcaacttg
     3301 acattagcat agacaacaat gggattactg gaaacatggg aatagcattg ctcgattttc
     3361 ccaatgggtg tgccagcccc cctacccttg ctgccaatgg ctctttttat tgtggccctc
     3421 caatcaatac ctattcgttc caaaacctta cccccggctc aactatatat ataatgatta
     3481 ccagtaccac agccggtgct ggcgatttta ccaatatgtg ccttactgaa caggaaggac
     3541 aaccaccgtg taacacaaac ggagattgcg gtactgctat tgctattgat attcccaatt
     3601 cagcagaacc tgtatgtgtg accggctgta atgtcggcat gccggatggc cctattctta
     3661 atggaggagg tgcttgtacc aatatggata atccagtggc atggtacagt ttcaattccg
     3721 gaaacaacaa taccgcagtt atcaacttca gttcgggtga ccttacatcg ccgatctatg
     3781 cattattcaa ttcctgcaac acatgggttg aatgtaatcc tgttagtgct tcagtgatac
     3841 ccaacacaac gtactatatt gcagtaaccg atgctgatgg cgcggaaggg aattttgaat
     3901 tatgcatcac acttttaaat attacagcgc cttgtatcac ctctcaaagt ttgaccatcg
     3961 ttggagctaa tatgggttca cctctgacag gtcctttcaa gaaatgcgaa gaggttactt
     4021 ttaaatatac gaccaatttt atcaagattg gctcccaatg gatccatagc atgttcccgg
     4081 ttattagtaa ctgcttcgat tatcaacaag gtacagagcc taccccttcc aacaggccta
     4141 cagggtcagc cacttgggat tggtatcccg ccaatactgt ccactggaaa tctttggaca
     4201 attcgtccgg agctgtaggt atcaatttgg ggaccggaaa tatttgtatt attggcacac
     4261 ccggttgtca ggcatttgtt ggcggcggca attgcagctg gactggtacg agtatgcctg
     4321 ccggttggat tggacctacc tatagtggga cttgtagtag ttcaattcca aatttatctt
     4381 atggagatcc tgcttcagga cccttttccg tgcaatttac cgtgaaaatt ccatgctctg
     4441 cctgtacaga tcaaagttgc aatgattaca ccgtagctat tactgcattc tctgatgggc
     4501 agaccggtgg atggcaaagc tcgtcctgta atggccacac cttgctaacc aagaagatca
     4561 cagtccaatg ttgtaccgaa cccaccatga cactgcagga tggccaaata tgcagtggtg
     4621 aaactttttt tgcaaacatc acactagacc cacccaactc tactcttcaa tggacggtgg
     4681 tcaatgccaa tggagtctct ggagctacat caggttccgg aagcacattt tcacaaacat
     4741 tgaccaataa tacaggttca tccaagacag ttatttattc tgttacaccg ataagtccta
     4801 ccggttgtgt aggccaaccc caaacactga acgttttagt atttccacag gtaattgcca
     4861 atgccgggcc ggacaaaact tcatgccccg gagccactgt aacccttggt ggcaacccga
     4921 ctgcatcagg aggagcaggt gctccataca ctattgcctg gtctacaggg gaaaacaccg
     4981 aaaatattgt agtttcacca accatcaata ctacttactc agtaactgtt acggatgcaa
     5041 atggatgtag tgctgttgac caggtaattg taagtgtaac cggaaatctt gaggtaaata
     5101 ttcttccaaa ccctgctgaa tattgcttaa atgaacttac aggaaagact ttgagttctg
     5161 cagtcaatag ccagaatgga ccctttacct acgaatggaa cacaccatgg ggacagttta
     5221 caacagcaac cattccaatc aatgccaccc aagccggaag ttttcaggtt actttacagg
     5281 ttacagataa atatggttgt aaaggaattg gcaccttgac aattaaaatc aatccaactc
     5341 caaaaatcaa tttcttgaac aagccggatc agccactatg tcctttagag gcatttattt
     5401 tgctgacaga cccttcttcg tcacaaggtg tagtttactc atcccatcct gatgggttta
     5461 ttttaccgga tggcactata ttggttgatc aaatgactcc aaatattcca tattggatag
     5521 ttgcagccta ttctgatcct gtaacgggat gcaaatccag agatagcata caagtttctg
     5581 ttcttcagat agatgaccca agtattacgc ctgccggacc tttctgtgcc agtgaaaatg
     5641 gtccttatca actcataggc acccccgctg gtggcttatg gaccggaggc gtttcatcag
     5701 atggattgtt tttcccttct gctttaggtc caggcatata taccattgaa tatacagttg
     5761 gcacagggac ctgtactaaa cagaccttca cgaccattga agttaaaccc aacccgacac
     5821 cctatatttt aaatctttca acatattgta atacagattc tcccaatccc atccttataa
     5881 cggatattcc gggaggaacc tggtcgtatc caatcacacc gcttggagaa atacccatta
     5941 atagtatttc tccgggaatt tatgccattt cgtattctgt aacaatagat gggtgtacag
     6001 gaaatgtgga tgatgtactt ataataaaca aacaaccgga agcaacgata ataaatactg
     6061 ctgtggtctg taatgccgat ccgggcaacg acggaaaatc aaaactcaat cttaatgatt
     6121 ttttcaccgg aggagatgca agtggatcat ggacagaaat tccaccactg agtggtgctg
     6181 ttaatcttgg tggaaacaac tatgatttca ccggattacc tgatgggact attgtccgat
     6241 ttgaatatat agtacccgcc tcacttccct gcaacgattc aagagacaca ttgactgtta
     6301 cggtggatga tgattgtgac tgtcctaaac tcctgttctc caaacccggt gacttgtgta
     6361 atgataacgc atctgtcgat atcaacactt ttataataaa tgcaggatcc ggtggatggg
     6421 ctctttattc agtgcctagt ggaggtaatc cgggaacatt gaacggaaca atatttgacg
     6481 caacaggcaa agacccggga aattacatca tcagatacac cttgaatccg gaacctacaa
     6541 atgccatttg tcctaaattc attgatctca caattacggt aaaccctgat gtagtgtaca
     6601 ccatgaaacc ggaaattaaa gtctgcaata atgtaccatt accaccttca ccaaaaacta
     6661 tggtaaatct ggatgatttc ttcacaagta atccggtacc aggaacatgg ataaataatg
     6721 ataatattgg tactaatgta ataggcaaca catgggactt cacaggtgta ccaataggtt
     6781 cttattcttt cacttttact tcttcaaatg cagttgcacc atgtcagaac aaatcattaa
     6841 ctgtaaaagt gactgtaaca gaaaattgca attgtcctga attgtccgta ctgactccga
     6901 tcactaccct atgcagtgat aatcagaata caatcgacct caactcactt atcgtcagtg
     6961 ccgaagcagg aaaatgggca cttgtcaatg atcctaccgg tcaagtctcc attccggttg
     7021 ccaatggatt ttttgaccct gccggaaagg ctgatggtgt atataccttt gaatttgaaa
     7081 tcgatatcac tccaccggaa aattgcgaca gcactgtgac agttgatatt gttatcgtgc
     7141 ctcaactggt atataagctt caggataatg ccactgtctg caataaagat ccaaaatcag
     7201 aaaatgcaga tcaacttgac tttaactcat ctgtaatctg gcaaagcaca ccggtttcag
     7261 gcatttgggt ggatacggat ggatcaggtg ccatccagac aggcaatatc tggaatttta
     7321 ataatgttcc tgttggcaca tatatattta catttgtgcc aagcggtgct ccatcgccat
     7381 gtgtaaatat tccacagacc attacaatca atgtagtgga taattgcgaa tgtcctgatt
     7441 tgcctgtgtt tgctccaatt cctgctacat gtaataacga atcagaaatt aatttgtcaa
     7501 tacctaacgg ttttacaggc acctggtcca tcaaatccag tccggcaggt tcgaatccat
     7561 tatcaatttc cggaaacaca gtcaatatca ataaaaagga tgccggcact tatgttttac
     7621 gatttatagt tgataatgtt aagcctggct gcccagattc tgtttttgta aatatcattt
     7681 tagatgactt caagtttgcc ggcacagaag aagctttggt aacattctgc gagaaaactt
     7741 caaccatcat agacctggct gatcacatta caggtgaaga ttccggtgga gtctggaccg
     7801 aagttggccc aagtactgca ggttcagcac tgataggtag tatattaaaa atcaaagatt
     7861 tgaaagccgg tgattatata ttccagtacc gctttgccaa tggtggaagc tgtcctgcga
     7921 gttcatcaga agtcaaagtt actatttttg aaaacccgat tgcagatgca ggtcaagatc
     7981 aggaacttac ttgcgaaatt tcccaggcaa cgataggaaa tccatcttct ccgggcaata
     8041 atctattata ccaatgggcc gaagtcagta ataaaccaat tccggatcca aatgcttcaa
     8101 gcatggtggt aaccatcgat ggtacttata ttttgactgt cactgaccct aagactggct
     8161 gttcggataa ggatacggta gttataactt ctgatccgga aagacccaaa gggattgaaa
     8221 tagaaagaga agggcctact tgttacggat ataaaaatgg ttatgtcaaa atatcaaaaa
     8281 ttgaaggagg tgctcctcca attctatatt ctttcaatgg aggtccatat tcatcgatta
     8341 attcatggtc caatttgtca ggaagcacca atataatcaa gattaaagat gcaaatggat
     8401 gcgaattgac acttgataat atcgtgttga tcgagccacc tcttgtaact gtagagcttg
     8461 gtaatgatac cctgatcaat attggtgata cagttatcat caaacccgac ataagcatac
     8521 cggaagacca ggttggttcg attacttata gctcagatta cgacttcatc aattgtatcg
     8581 ggtgtttcca tataacagtt tatccaaacc agacaactgt ttataccgtt gaggtaaagg
     8641 ataagaaggg ttgtagtgac aaggactcta agaaaatagt ggtcaagaaa ggtgtcaata
     8701 tttttattcc caatattttc acgccgaatg gagatggaaa taatgacaag gtatttattt
     8761 ccactaatga taaggaaata aaacgaatcc tcagcttcca gatttatgac cgatggggtg
     8821 agaaagttta tgaagccaag gattatcagc caaatgatcc aaacaacgga tgggatggaa
     8881 ccttaaaggg acagaagtgt aatccgggtg tttttgctta ttggactgag attgaattat
     8941 ttgatgggac ccgacagata ttaaaaggtg atgttacctt actaagataa atccttaaac
     9001 aaaacgtact ttcaggcagt gttcttatgg atactgcctt tttttattcc tccgcaagcc
     9061 ttactatcaa accatcgagt tgctctgtaa tcttcaattg acagctcagc cgactgttat
     9121 tctccatatt gggcagctgg tcaagcataa tttcttcatc tgcccccatc tccggcaacg
     9181 tcgtactgct ttcaaccaaa acatgacagg tagcacaaag ggccatccct ccacaagttg
     9241 ctaaaatagg taaatcattc gccttacaca gctccatgac agaaattccg atatccgggg
     9301 gtatatcata tatatgttcc tgcccttccc tatctattat ggttatccta atttcatctt
     9361 tcataaaata ccttcaagta ttcaaattaa tgtttggcaa aacccaatga ataatcaaat
     9421 gtaccaaata tttatttgtt aatttatatt agtatccgct ttatcatagt ataaatgatt
     9481 ttaggttgaa agtgttcact ctgggattat aaaagactaa ttaccttcaa cattacattg
     9541 aattttacat taaatcaatc agactcaatt caatttccgg ttttcatcca atgatcttcc
     9601 tttattatta tagccaaaat gctgaaaata gtatttttcc gactaattgg ttgtaccaga
     9661 tatcggattt caatattatc tttatacatc ggagataata gcttagctta aacaaccggt
     9721 aaaatagtta cattcatttt tttaaatcaa taataatgcc attattcaaa ataaacccag
     9781 caaatacctc ctcctggaaa tctttgtctt catcgcaaaa acgtttggaa ggagtcgata
     9841 tcaaaactct tttcagcgct gacgaaaaca ggtttgacaa ctattccatt tctttcggcg
     9901 atattatgtt cgactattcc aaaaacagga tagacggaca ggtcatggcg gaattattgg
     9961 ggcttgctga agaatgtaag gttcgggaaa gcatcgatgc tatgtttggc ggggagaaaa
    10021 ttaacgaaac cgaaggacga agtgtattac atatcgctct ccgaaacaga tcggacagac
    10081 ctatattgag taacggaaat gatgtaatgc cggaaattcg aagagttttg tctaaaatga
    10141 agactttctg tcacaaaatc cacagtggtg cgcacaaggg atttacaggt aagaaaatca
    10201 agcatatcgt caatattggc attggaggta gtgatctggg acctgtaatg gttaccgagg
    10261 cacttcgtcc atattggtta gaaggaatac aggcacactt tgtttctaac attgatggga
    10321 cgcatatcgc tgaagtatta aaggtaatta atccagaaac gacgcttttc ttgattgctt
    10381 ccaagacatt tacaacacag gagaccatga ccaacgctgc aacagcacgg gattggttcc
    10441 tgagcaaagg tggaagcagt caggatatcg cacttcactt tgcagctctt tccaccaatg
    10501 agaaagcagt ggaagctttt ggcatcaata ctgagaatat gttcgaattc tgggattggg
    10561 tcggtggtcg atatagcctg tggagcgcca tcggcttgtc catcgctctg accatcggat
    10621 ttgaccactt tgaacaattg ctggaaggtg gatattccgt ggatgagcat ttcaagaaca
    10681 cagtgtttga aaagaatatc cctgtaatca tgtcgcttat cggtatctgg aattgcaact
    10741 ttttaggtgc acacacagaa gccatcctgc cctatgatca gtatatgcac cgcttccctg
    10801 catacttcca gcaaggtaat atggaaagca acggtaaatc cgtcgcccgt gatggaaatg
    10861 aagtgactgt ggagacagga ccgatcatct ggggcgaacc gggtaccaac ggtcagcatg
    10921 ccttctacca actgatccac cagggaacca gactcatccc ctgcgacttt attgcaccag
    10981 cgatctcaca aaatccattg cccgggcatc acgataagct cctgagcaat tttttcgctc
    11041 aaacacaggc tctcgccttt ggaaagagt
//
LOCUS       DAOQQN010000053        10982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2152_length_10982_cov_6.281413, whole genome shotgun sequence.
ACCESSION   DAOQQN010000053 DAOQQN010000000
VERSION     DAOQQN010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10982
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2152_length_10982_cov_6.281413"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(299..1294)
                     /locus_tag="PLG24_03250"
     CDS             complement(299..1294)
                     /locus_tag="PLG24_03250"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HMZ23932.1"
                     /translation="MDFTQEQISELLLTIANGKDGYRMLMQQSLEAIMRAERKEFNEV
                     HSDYGNGYRFSKICVHNGKMELMIPRSRHHNFYPLILALIKDQNKESEALAFELYKSG
                     LTTEQVGKLFSKLYDHHYSKSAISQMMKSARQDVFSWLERKGLDEIYPVVFIDATFWP
                     TRRENEVSNEAYYTILGVKQDTTREVLSLVNHPTEGASNWEDALIQLKQRGVKRIELV
                     VVDGLNGIEDVINRVFPEAKIQLCTVHLTRNIVSKVKPVDKLQVVKDLKEVLDPELPD
                     DNPSKGIIRFNEFTRKWQSKYPSFKQYLRPKYALYFTYLNYEKEIRRMIYTTNWI"
     gene            complement(1443..2096)
                     /locus_tag="PLG24_03255"
     CDS             complement(1443..2096)
                     /locus_tag="PLG24_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23933.1"
                     /translation="MNKLILFLCTFLPLLAAAQVTESSKVMSQGSNNALTVSLTDISV
                     SDAEKVWKDFAKTFGGKIKYNKKEKEYFVDDASVPSVSTNTIDLYSKAEKVGTEVAYS
                     VWFDLGGGYLSSTSNAAMYSNAVSFMKDFLREVERFKINEQLKIEMKSLEKLNDNLKS
                     LTRDKEGYEKDIKKAEEKITESKAKIEQNIKDQASKQSEINAQQSKIDKIKSDLSKL"
     gene            2321..4162
                     /gene="mutL"
                     /locus_tag="PLG24_03260"
     CDS             2321..4162
                     /gene="mutL"
                     /locus_tag="PLG24_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HMZ23934.1"
                     /translation="MSDRIKLLPEVLANQIAAGEVVQRPASVVKELMENAIDAGATNI
                     SLILKDGGKMLISVIDNGVGMSPIDARMSFERHATSKLSSVKDLFAIRTMGFRGEALA
                     SIAAVAQVEMTTKRAEDEVATRIVNEGNKITTQEATQAPTGTTIAVRNLFFNIPARRA
                     FLKSTDVERRHAYEEFIRLALAHPDIRFQLVHNDEIIHFLEPSNLKLRVVNLFKNDID
                     KRLLSVEEETDKLKIFGVIGTPQQSRKTRGDQYLFVNHRFIKSPLLNHAITSAYDKII
                     PSDHFPFYILFLELDPATIDVNVHPTKQEIKFDDEHIIYTYLKAAVKHTLSYNLVAPT
                     LDFEKDEQLSRAWNNEARSPHIPPANPVNQRPGENYRIPQEFIKENVGRWKDLYENIQ
                     INPEPEPKSDQPLIFNSSINEEKSISEDEEMPSVTYLQVHRSYIIFQVKSGLMIIDQH
                     NAHHRILYEQFYTKTREAQVITQTNLFPTTLYFDVVKADLLKTILDQIGTIGFDIEIS
                     GTNEFIVHGYPSFLAEGLDLQGWFDELIDTLYQGVEVNFDMHHVMAMAYADRKALPRG
                     KELKQREISELVDQLFACTEPSYDARGNSCFGRCTLLELEKIIINKK"
     gene            4159..4764
                     /locus_tag="PLG24_03265"
     CDS             4159..4764
                     /locus_tag="PLG24_03265"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMZ23935.1"
                     /translation="MIRITNVVKQLIILNVIVFIIVSFSPELKGMLSLFFPTSPNFRV
                     WQPLSHMFNHNDIGHIFFNMFGLYIFGSLLESVWGEGKFLRFYLFSGIGAFIMQWAFW
                     YFTAGPNLNYVSMLGASGAISGCLMGVAVLAPNMQVMLLFPPIPLKMKYLAIGYFCLD
                     LFMGLGGGGNVANFAHIGGALTGLAISFYWKSRGFLHRRGN"
     gene            4813..5718
                     /locus_tag="PLG24_03270"
     CDS             4813..5718
                     /locus_tag="PLG24_03270"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMZ23936.1"
                     /translation="MFDSIIRDIRYELTRGNTITKLILVNIGVFIVFLLTKVISMAIT
                     GGDESLYGQILSWFALSGDFRKLIIHPWTIITYSLVHLSIWQILFNMLLLYWFGQIAG
                     DLVGDRRIFPIYLYGAIVGGLLAVSAGSLLPTFNGKDFLILGAMAPVMAIIVAAATLA
                     PDYSMRLILIGPVRIKFIVLIIIVFLVAGLSTKFAAGSSYAGLGGALTGYLFIIALRN
                     GYDPTGLFRKPSRVSIGIKRQSDKKSKIISIFDTIKKHETELRENHPDHTAKRLDLIL
                     EKIKKNGRSSLSQDELDFLDKMSKS"
     gene            5720..6799
                     /locus_tag="PLG24_03275"
     CDS             5720..6799
                     /locus_tag="PLG24_03275"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HMZ23937.1"
                     /translation="MKILRNIFIILNIVAAMLLALSYFSPVINPYEHWQFGILGLMYP
                     FMVFTNLAFAAFWLFRKKWWFLISMVTILVGWNSFNGIIGFKQPQDTKDKDHTIRIMS
                     YNIRALSNLVKYPTPSLKENREKFAFFIEENKLPHVLCVQESTIPNINWFKANLAYPY
                     TCRYPGPNSNTAILSVFPIEENGVVKFDRGNGDCVWADINFNGQIIRFYSIHLHSNTI
                     SVQADTLLTMHKLEGEETLKGVEGMFKKYKLATKVRYRQAMKIKNHAESSPYPVVLCG
                     DFNDTPQSFIYHNLATGLKDAFKERGIGFGTTWAGNIPGLKIDYILVDPGIAVLSHDI
                     YRRPFSDHYPVKATILLPEKAKTKK"
     gene            6802..7539
                     /gene="truB"
                     /locus_tag="PLG24_03280"
     CDS             6802..7539
                     /gene="truB"
                     /locus_tag="PLG24_03280"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HMZ23938.1"
                     /translation="MQKTTLDNLPRELVAYQEGVLLLIEKPLNWTSFDVVNRIRVALC
                     KYFGIKKLKVGHAGTLDPLATGLLIICIGKFTKRIDEIQAQEKRYSGIIRVGESTPTY
                     DLESEIDKIHPIDHVTPIMIEQVRKQFVGEIMQLPPIFSAIKKDGKRAYELARKGINA
                     GLEARPIVIHDLKFDDTSWPDLFFEVTCSKGTYIRSLAHDVGVALDSGAHLKSLHRAG
                     IGEYDNKDAIRLEDFLEVFGKRKVAIQ"
     gene            7974..9272
                     /locus_tag="PLG24_03285"
     CDS             7974..9272
                     /locus_tag="PLG24_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ23939.1"
                     /translation="MRIAVPILFVLLAISPLLAQNYTIYHTGNSKDTTTQAQGGICMM
                     GGSSEDDNAMRWFLQRAEGGDILVLRSSGSNGYNNYLYSKLGVKVNSVETIVCNNKNA
                     SFESYIHEKIAGAEAIWFAGGDQGEYVRYWRGTPIDSLVRDAISRRNIVVGGTSAGMA
                     IMGKYIYTGRNGSVTSAEALANPYNTRVTIDSARFMDNRFLENVITDTHYDSPDRRGR
                     HITFMARILTDWASESRGIACDEYTAVCIDENGIARVFGEYPTSNDNAYFLRPNCGLS
                     DYTPETCAKGQPLQWLHSKKAVWVYKVKGNKEGSNTFDLNDWITGNGGEWEDWYVDQG
                     VLAMQPTDQVDCTPLETYDNQQSDIRIFPNPTSSLLTLESSGRRISDVFLLDLQGNIL
                     KRGNLLDTSIVFDVTELVSGIYFLRIVTEKGIAIRRVVKM"
     gene            complement(9683..10348)
                     /locus_tag="PLG24_03290"
     CDS             complement(9683..10348)
                     /locus_tag="PLG24_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HMZ23940.1"
                     /translation="MKTKKLLVELVECVSLFESENPHLERYDLQGFMAFLQSRSVTGK
                     QEHFAPRKIAGHRKPHGFHLHENPANILSRLIGLIYRYAREYTKKALEGSSLQTVEEF
                     SFLVVLMTYDSLTKTELILKNAMVKTSGTEVVKRLLKKKLIRQFDDINDKRSQLVAIT
                     GQGLAEMKIVFPRMHLASDIIAGNLDPAELHTLVFLLQKLEAYHNELFLGRKDADLED
                     LMK"
     gene            10506..>10982
                     /locus_tag="PLG24_03295"
     CDS             10506..>10982
                     /locus_tag="PLG24_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23941.1"
                     /translation="MLKNLPEPIKYFEEYIYLDAKHVIQIHILSTLDFYLELPFNSSV
                     PTIFEVMKRLFLSNILILLVLNLLVKPLYIFGIDVQVQNSIGPEQYGLFFSIFNYTLL
                     FQFVIEYGITNYVKREVSLDNRRAPTVFSGILLLKLGLALLYFLLIIAFAWAFNYTG"
BASE COUNT         3085 a         2355 c         2294 g         3248 t
ORIGIN      
        1 atttgcccaa tattcattcc cggaaaagta aaagaaagga gcataaggta atgttcaaaa
       61 aagtgtgtat tttttttaat tatgaaaaat tcaaggagcc caaaaaaaag aatcctgtaa
      121 ttcatgttta aaattaagta tttataaaac tcagtttttt agttaattta aattggtaaa
      181 tagggtagtt aaattctggt ctgtccatcg ctacgctacc taataaaaaa agaacagatt
      241 ccggatttgg cattgagctt ctcattctca gtgttctttt ataatttcgg tttaatcgct
      301 aaatccagtt tgtggtataa atcattcttc ttatttcttt ctcataattc aaataggtga
      361 aatagagtgc atactttggt cttagatatt gcttgaatga tggatattta gactgccatt
      421 ttcttgtaaa ttcattaaat ctgattattc ctttggatgg gttatcgtct ggaagctccg
      481 gatctaagac ctcttttaaa tcttttacta cctgtagttt gtctaccggt ttcactttgg
      541 atacaatgtt tcttgtcaga tgcactgtgc acaactgtat cttagcttcc ggaaataccc
      601 ggttaataac atcctctatg ccatttaatc catccacaac taccaattct attcttttta
      661 ctcccctctg ttttagttga attaaggcat cttcccaatt agatgcccct tcagttgggt
      721 ggttgaccag actcagaact tctcgtgttg tatcctgctt tacgcctaat atagtgtaat
      781 aagcttcatt actcacttca ttctctctac gtgttggcca aaatgtggca tctataaata
      841 ccacaggata tatttcgtcc aggcctttcc tctctaacca actaaataca tcttgtctgg
      901 ccgatttcat catctgacta atcgccgatt tactataatg atgatcatat agtttggaaa
      961 acaacttacc tacttgctct gtagttaatc ctgacttgta tagctcaaat gctaatgcct
     1021 cgctctcttt attctgatcc tttatcagcg ccaatatcaa tggataaaaa ttgtgatgtc
     1081 gactcctggg aatcatcaat tccatcttac cattgtgtac gcatatctta ctgaatcggt
     1141 atccgttgcc atagtcagaa tgtacctcat taaattcttt ccgttccgct cgcataattg
     1201 cttctaatga ttgttgcatt aacatacgat atccatcttt tccatttgct attgtcaata
     1261 atagctccga aatttgttct tgtgtaaagt ccattttttt ctgattttgt tttgttatct
     1321 acaaaaatct taatttttgg ctttacacac ttttttaaac agtatcgagc ataaaatatc
     1381 catccggcat tatgctccct tcagttcgcc aggccagtgg catggcagat tataattgag
     1441 atttacagtt tgctcaagtc ggatttgatc ttatcgattt tcgactgctg agcatttatt
     1501 tctgattgct tacttgcctg gtcttttatg ttttgttcga tcttagcctt agactcggtt
     1561 attttttctt cagctttctt aatatccttt tcatatcctt ccttatcgcg agtcaagctc
     1621 ttcagattat cgttcagctt ctcaagtgat ttcatttcaa tcttgagctg ttcattgatc
     1681 ttgaagcgtt ctacctcccg caggaaatcc ttcatgaagg atactgcatt ggaatacatg
     1741 gcagcattgg aggtagagga aagatagcct ccacccagat cgaaccacac actatatgcc
     1801 acctcagttc ctaccttctc ggcttttgaa tagagatcaa tggtgttggt acttacagaa
     1861 ggtacgcttg catcatcgac aaagtactct ttttccttct tgttgtattt gatttttccg
     1921 ccgaatgttt ttgcaaagtc cttccaaact ttttctgcat ccgaaacact gatatcagtc
     1981 aaggaaaccg tcaaggcatt attgcttccc tgagacatca ctttactgct ttcggttacc
     2041 tgagcagctg ccagcaaggg tagaaatgta cataaaaaaa gaattaattt attcatgtca
     2101 tctggttttt atcattataa atatttagac ctttgttgat caaaaaggta acaaaaccta
     2161 tgttcaactt ttgaaacaat atttacaaaa ttattgtttt cattatcttt ttccattgtt
     2221 ttagcaatat tcattttata aaatcttaca ttgcaaaacg ccaacgttta aatttacagt
     2281 tgatgttgac tacctttgac ccatcaatta attgataccc atgtcggatc gaatcaaatt
     2341 attgcctgaa gtattggcca accagattgc cgccggagaa gtagtgcagc gccctgcctc
     2401 cgtagtcaag gagttgatgg aaaatgccat cgacgccggt gcgaccaata tcagtctcat
     2461 tctgaaagat ggtggaaaaa tgttgatttc cgtgatcgac aatggtgtgg gtatgtctcc
     2521 gattgatgca aggatgagct tcgaacgaca tgccacctct aaattgtcca gtgtcaaaga
     2581 cctgtttgcc atccgaacca tgggattccg tggtgaggca ctggcctcca ttgcagcagt
     2641 ggcccaggtg gagatgacta ccaaaagggc agaggatgaa gtggccacac gcatcgtcaa
     2701 tgaaggcaat aagattacca cacaggaagc gactcaggcc ccaaccggca ccaccattgc
     2761 ggtgcgtaac ctgttcttca atattccggc cagaagggca tttctcaagt ccaccgacgt
     2821 cgaacgccgt cacgcctacg aggaattcat tcgcctggcg ctcgcacatc ccgacattcg
     2881 gtttcaactg gtacacaacg atgaaatcat tcactttctt gaaccgtcca atctgaaact
     2941 gcgtgtagtc aatcttttca aaaacgatat cgacaaacgc ctgctctcgg ttgaggaaga
     3001 aaccgataaa ctaaaaatat tcggtgtcat aggcacccct caacaatcgc gaaaaaccag
     3061 aggcgatcag tatctctttg tcaatcaccg atttatcaag agtccccttc tcaatcatgc
     3121 gattacttca gcttatgaca agatcatacc atcggatcat ttcccgttct acatcctctt
     3181 tctggagctg gatccggcga ccattgatgt caatgtccat cccaccaaac aagagattaa
     3241 atttgatgat gagcatatca tctataccta tctcaaagca gccgtcaagc ataccttgtc
     3301 ctacaacctt gtggctccga cgctggattt tgagaaagat gaacaactgt cccgggcctg
     3361 gaacaatgaa gcccgatcac cacatatacc accggccaac cctgtaaacc agcgacccgg
     3421 ggaaaattac cgaattcccc aggaattcat caaggaaaat gtgggtcgtt ggaaggattt
     3481 gtacgaaaac atccaaatca acccggaacc agagcccaaa tcagatcaac cacttatatt
     3541 caattccagc atcaatgagg aaaaatcgat atcggaagat gaagagatgc cttccgtgac
     3601 ctatcttcaa gtccacaggt catatatcat ctttcaggta aaatccgggt tgatgattat
     3661 cgaccagcat aatgcgcatc acaggatact gtacgagcag ttttatacaa aaaccaggga
     3721 ggcccaggtc attacccaga ctaatctttt tccaacaaca ctatactttg atgtcgtcaa
     3781 ggcggatctg cttaaaacaa ttttggatca gatcggcacc atcggctttg atatagagat
     3841 aagcggcacc aatgaattca ttgtgcatgg ctatccatcc ttccttgctg aaggcctgga
     3901 tttgcaaggc tggttcgatg agttgattga caccttgtat caaggggtgg aggtcaactt
     3961 cgatatgcac catgttatgg caatggccta tgctgatcgc aaagctcttc caagaggaaa
     4021 ggagttgaaa caaagagaaa taagcgagtt ggttgaccaa cttttcgcct gtacggaacc
     4081 ctcttatgat gcgcggggca attcgtgttt tggcagatgt accttactag aattagagaa
     4141 aattataata aataaaaaat gattcgaata acgaatgttg tcaagcaact gatcatactc
     4201 aatgtcatcg tatttataat cgtcagcttt tcaccggagc taaagggtat gctaagcctt
     4261 ttctttccta catcgcccaa tttcagagtc tggcaacctt tgagccatat gttcaatcac
     4321 aacgacatcg ggcatatctt tttcaatatg tttggattgt atatatttgg ttccctgctg
     4381 gaaagtgtgt ggggagaggg taagtttctt cggttttact tgttcagtgg aatcggtgcc
     4441 tttataatgc agtgggcctt ttggtacttt acggcaggtc caaatctcaa ttacgtcagc
     4501 atgctcggag catcaggagc gataagcgga tgtctgatgg gtgttgctgt actggccccc
     4561 aatatgcagg tcatgctcct gtttccgccc attccattga agatgaaata ccttgccatc
     4621 ggatattttt gcctggacct tttcatggga cttggaggag gaggaaatgt agccaatttt
     4681 gctcatatag gaggagcact gacaggtctt gccattagtt tttactggaa gtcaagaggg
     4741 tttctacaca gacgtggcaa ttaaaaaatc aggaatatca aggatgatca agaccattaa
     4801 ccatttcgat ccatgtttga ttcaataatt agggatatcc ggtatgaact gactcggggc
     4861 aacacgatta ctaagttgat attggtcaac atcggagtgt ttatagtatt cttacttacc
     4921 aaagtgatca gcatggccat aaccggtggt gacgaaagcc tgtacggcca aatcttatcc
     4981 tggtttgccc tttcgggaga cttccgcaaa ttgattatac atccgtggac catcatcacc
     5041 tatagcctcg tccacttaag catctggcag atcctgttca atatgctttt gttgtattgg
     5101 tttggccaga tagcagggga tttggtcgga gaccggagga tcttcccaat ctacctttac
     5161 ggtgcaatcg tgggtggctt gctggcggtt tcggcaggta gtcttctgcc gaccttcaat
     5221 ggtaaagatt tccttatact gggagcaatg gctcctgtca tggcgattat tgttgcagca
     5281 gccactttgg ctccggatta ttccatgaga ttgattctga tcggtccggt caggataaaa
     5341 tttatcgtac tgattatcat agtctttctt gtggccggcc tttcgacaaa atttgcagca
     5401 ggcagttcct atgccggcct gggaggtgcc ttgaccggat atcttttcat tattgcctta
     5461 cgaaacggtt atgatcctac cggattgttc agaaagccaa gtcgtgtgag cataggaatt
     5521 aagcgtcaat ctgacaaaaa gtctaaaatt ataagcatct ttgatacaat aaaaaaacac
     5581 gaaacagaat tgagagagaa tcatcctgat cataccgcaa aaagactgga tctgattctt
     5641 gagaaaataa agaaaaatgg ccgatccagc ctaagccagg atgagctgga ctttctggac
     5701 aaaatgagta aatcataggg tgaaaatact cagaaacata ttcatcattc taaatatagt
     5761 ggcggcgatg ctgctcgcac tttcctattt ctctccagtc atcaatcctt acgaacactg
     5821 gcaatttggc atcctcggcc tgatgtatcc attcatggtg tttaccaatc ttgcctttgc
     5881 tgcattctgg ctgtttagaa agaaatggtg gtttctcata tcgatggtta ccattcttgt
     5941 cggctggaac tctttcaacg gcatcatagg ttttaagcaa cctcaggaca ccaaggataa
     6001 ggaccatacc atccgtatta tgtcctacaa catcagggcg ttatccaatc tggtaaaata
     6061 tccgacaccg agtctgaagg aaaaccggga aaaatttgcc ttcttcattg aggaaaacaa
     6121 gctgccacat gtcctttgtg ttcaggaaag caccattccc aacatcaact ggtttaaggc
     6181 caacctggca tatccttata cttgtcgata tccgggtccc aacagcaata ctgccatatt
     6241 gagtgtattt cccattgagg agaatggcgt ggtaaagttt gatcgtggta atggagactg
     6301 tgtatgggcc gatattaatt tcaatggtca aatcatccgt ttttacagca tacatctaca
     6361 ctccaatacc atcagtgtcc aggcagatac actattgacc atgcataagc tggaggggga
     6421 ggagaccttg aaaggtgtag aaggcatgtt taagaaatat aaacttgcca caaaagtcag
     6481 ataccgtcag gcgatgaaga taaagaacca tgctgaaagt tcaccctatc ctgtggtgtt
     6541 gtgcggtgac ttcaatgaca caccacaatc ctttatatat cacaaccttg ctacaggctt
     6601 gaaagatgct ttcaaggaac gcggtattgg atttggcacc acctgggcag gaaacatacc
     6661 cggcctcaaa atagactaca tcttagttga tccggggatt gctgtcctaa gccacgatat
     6721 ctacagaagg ccattttctg accattatcc tgtcaaggcc accattttac ttcctgaaaa
     6781 agccaagact aaaaaataat tatgcagaaa acgactctgg acaacctgcc acgcgaatta
     6841 gttgcttacc aggaaggtgt tttattactc atcgaaaaac ccctcaactg gacttcattc
     6901 gatgtggtca atcgcattcg ggtggctttg tgtaaatact tcggcatcaa aaaactgaag
     6961 gttggccatg caggcacact ggatcccctg gcaaccggct tactcattat atgtattgga
     7021 aaattcacca aacgaatcga tgagattcag gcacaggaaa agcgatattc aggtatcatc
     7081 cgcgttggcg aatccactcc cacctatgac ctggaaagtg aaatagacaa gatccaccca
     7141 atagatcatg tcacccctat catgatagaa caggtcagaa aacaatttgt cggagagatc
     7201 atgcagcttc caccgatctt cagtgccatc aagaaagatg gcaagcgggc ttatgaactg
     7261 gcgagaaagg gtatcaatgc aggtctggag gccagaccta ttgtgatcca tgatttgaaa
     7321 tttgatgata catcatggcc ggatttattt tttgaggtta cctgctccaa agggacatat
     7381 atccgatcgt tggcccatga tgtaggtgtt gcgctggata gcggtgctca tctcaaatca
     7441 ttgcaccgcg ccggaatcgg cgagtatgac aataaggatg caatcagact ggaggatttc
     7501 cttgaggtat tcggaaaaag aaaagtcgct attcaatagc ccccggcggg atagggatta
     7561 atcgttgcca taagttttaa gcacattggt acaatgcatc aaaatgaagc aatcacattt
     7621 tcggtgaaac tgtttattta caaccaatca ttactctatt gatgaattga tgatacaaca
     7681 gatgctacaa cgccattaat ccggtgctta ctcacaactt gtgcattact tgtatcaaaa
     7741 gaagtattgc gccatcgaaa aaatagacct attgaaaaat ggtggtcatg aattgataaa
     7801 attctttgat atcgatttat aagcggagaa caagaccaat tcagtacaaa acattccttg
     7861 tctgtatggt tttttcattt gtgcagtttg cgatgtgccc aagtaccttc accgacctgc
     7921 gaattccata cattttacta actttgaatt aaataaaaga taaagagaat cgtatgcgta
     7981 ttgctgtacc catcctgttt gttttattgg cgatttcgcc cttgttggcc cagaattaca
     8041 ccatttatca tacaggtaat tcaaaagata cgactacaca agctcaaggc ggcatttgta
     8101 tgatgggtgg atcgtcagaa gatgacaatg ccatgcgttg gtttctccag agggctgaag
     8161 gtggcgatat tctcgtactt cgttccagcg gatccaatgg ctacaacaat tatctgtata
     8221 gcaagctggg agtcaaggtc aacagcgtag agaccatcgt gtgcaataat aaaaacgctt
     8281 ccttcgaatc gtatatccat gagaagatag ccggtgctga agctatatgg tttgccggag
     8341 gtgatcaagg ggaatatgtg cgctactggc gcggcacccc aatagattca ttggtaaggg
     8401 atgcgatcag ccgtcgcaat atcgttgttg gtggcacgag tgccggcatg gccattatgg
     8461 gtaaatatat ctataccggc aggaacggat ccgtcacttc ggctgaagca ctggctaatc
     8521 cctataacac acgtgttaca atagactcgg ctcgcttcat ggataatcgt tttctggaaa
     8581 atgtaattac cgatacccac tacgacagtc cggatcggcg agggaggcat attaccttca
     8641 tggcgcgcat cctgaccgat tgggcgagtg agtccagggg aatcgcctgc gacgaatata
     8701 cggcagtctg tatcgatgaa aacggcatcg cccgtgtctt cggggaatat ccgaccagta
     8761 atgacaatgc ctatttcctc aggcccaatt gtggactttc ggactacaca ccggagacat
     8821 gtgccaaggg acaacctcta caatggttac acagcaaaaa ggcagtatgg gtgtacaagg
     8881 tcaaaggcaa taaggaaggc agcaatacct tcgacctcaa cgactggatt accggcaatg
     8941 gcggcgaatg ggaagattgg tacgtcgatc agggtgttct ggcaatgcag ccgacagatc
     9001 aggtggattg tacaccgctg gaaacttatg ataaccaaca gtctgatata cgcatctttc
     9061 ccaatcccac atccagcttg ctgactcttg aatcatcagg tcgtagaatt tctgatgtat
     9121 tcttacttga cctacaaggc aatatcttaa aaaggggaaa tcttttggac acatccattg
     9181 tatttgatgt gacagaactt gtttcaggaa tttatttcct acggatcgtt acagagaaag
     9241 gaattgctat caggagggtt gtgaagatgt aaatttcgta agggaagttc gatgattggc
     9301 actaaatgga aggttcagtt ccggttgatg tgaagcgaag catttataga aatgatatag
     9361 atgcattaag gccgcccggt gtgggttgaa tataataaga tgaaaagtga ctttaaatat
     9421 ctgatcttat accaagatga tttatataat aatccttaaa tttttggtat acttgtccgg
     9481 atagctatgc tgctagagct ctgctctgtc ccgggagtga aacaatccgg ggttccggga
     9541 tgccgaggtt acagtcgtat agatcaatga aattggtcta taacgaatgt accttcggta
     9601 ttaatatggt caggcattga aatggtgaac tttattttct tccaaacaat aaattgtaca
     9661 acataccgaa tttttacttc aatcacttca tcaaatcttc aaggtcggca tccttcctgc
     9721 ctaaaaacag ttcattgtgg taggcttcca gtttttgcaa caaaaagacc agtgtatgta
     9781 attcggcagg atccagattc cctgcgatga tgtcggaagc aagatgcatt ctgggaaaga
     9841 ctattttcat ctcggccagg ccctgccctg tgatggcgac aagctggctc cgtttgtcat
     9901 tgatgtcatc gaattgtcgg attagcttct tcttcagcaa tcgtttgact acttccgtac
     9961 cggaagtctt gaccatggcg ttcttaagta tcagttctgt cttggtcaga ctgtcatagg
    10021 tcatcaagac gacgagaaag gaaaactcct ccacggtttg caaggaactt ccctccagtg
    10081 cctttttggt gtattcacgt gcatagcggt atatgagtcc aataagccgg gaaaggatgt
    10141 tggcgggatt ttcatggaga tggaagccat ggggtttcct gtgtccggct attttgcgtg
    10201 gggcaaaatg ttcctgtttg ccggtcacac tgcggctttg aagaaatgcc atgaagccct
    10261 gcaagtcata tcgctccaga tgaggatttt cagattcgaa taggctgaca cactctacca
    10321 attcaaccag caatttcttt gtcttcatac aactgacaca ttatttacac aaagatagga
    10381 caaatggtat gttttcgtac taaatttgat aagataaagg aagcttagct ggtgacagcc
    10441 ctcatttaaa aaactttctg actcgtcatc aacaagtgca aattgacatc cattcctttc
    10501 tacatgtgtt aaaaaattta cctgagccaa ttaagtattt tgaagagtat atctatcttg
    10561 atgcgaagca tgtcattcaa attcacatct tatcaaccct tgatttttat ttggaattac
    10621 cgttcaattc atccgtgcca actatctttg aggtgatgaa aaggcttttc ctgtccaata
    10681 ttcttatttt actggtgctt aacctgttgg tcaaaccttt gtatatattc ggtatcgatg
    10741 tccaggtaca gaatagtatc gggcccgaac aatacggttt atttttcagc atcttcaact
    10801 ataccctgct gtttcaattc gtcatagaat atggcatcac caattatgta aagcgggagg
    10861 tcagtttaga caaccgtcgg gcgccgacag tcttttcagg tatattgtta ttgaagctcg
    10921 ggttggcgct gttgtatttc ctgttgataa tagcctttgc gtgggctttt aattataccg
    10981 gt
//
LOCUS       DAOQQN010000054        10982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2153_length_10982_cov_5.442848, whole genome shotgun sequence.
ACCESSION   DAOQQN010000054 DAOQQN010000000
VERSION     DAOQQN010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10982
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2153_length_10982_cov_5.442848"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            339..1466
                     /locus_tag="PLG24_03300"
     CDS             339..1466
                     /locus_tag="PLG24_03300"
                     /inference="COORDINATES: protein motif:HMM:NF037744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23942.1"
                     /translation="MVELSELLIDLIFKQKSATVPELGTFEFVANPARIHFGENIIYP
                     PSQQLVFSDGKMDTGEVGFTEFLVQEKGFDEHEALIRVKAYVHKIKGNLDTLGYSYLP
                     GLGTLHRSENSSLEFTPGDKIKAMKPTLGLPEIKVEPIVREYVDPDKVVRETEVAGPV
                     NDLGDDSRSSKSWVLPVVLAFILVGLSVTGYYLYKMQSDNKGPQEANPVEIPADTAGV
                     EDNQASAVPSADDAGLNGMTDDEGSTYQPDDIVTDSNLSETNTQGISNAETKTETSAS
                     SSSGSTKPVKKVEKKAEKPLEDTPYTGKVCAIIVGVMSKPENARRLARTITNAGFQPF
                     AYTSKGLTRVGATCNCDDQSIDSTLRQMKKISADAWLYESE"
     gene            complement(1476..2066)
                     /locus_tag="PLG24_03305"
     CDS             complement(1476..2066)
                     /locus_tag="PLG24_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ23943.1"
                     /translation="MNHELLVNNIRRKIDLSEKEQAYILELFTEKKLKRKQFILHEGQ
                     ANHHLSFVVNGCLRAYSVDKNGFEHILQFAPNDWWITDIHSFVTGMPASLNIDAIEES
                     TILMITREDREKAFSLVPKLERYFRILMEKNLAANRQRIIDRLSLTAKERFVSFCNTY
                     PELINRIPQKQMASYIGVTPEFLSKMRSEYLRSLKH"
     gene            2511..2876
                     /locus_tag="PLG24_03310"
     CDS             2511..2876
                     /locus_tag="PLG24_03310"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HMZ23944.1"
                     /translation="MATNILGQAFEPATPPTSDEKTMALLSHLLVLVASFVAPLVIYL
                     LKKDESSFVANNAKESLNFQITIAIAIGVSVLLMMVLIGFLLIFAVSLANLVYVIIAT
                     MRASEGKEYQYPFCIRLIK"
     gene            3012..3569
                     /locus_tag="PLG24_03315"
     CDS             3012..3569
                     /locus_tag="PLG24_03315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HMZ23945.1"
                     /translation="MLNLKIIHASTREGRRGLPVSKWVVDAARKETDFEVEFIDLKEL
                     GLPFIDEPEHPRLKQYVHRHTVEWSNKIDHADAFIFVTCEYNHGMPATLKNALDFLVQ
                     EWAFKPVAFVGYGGISAGTRAIQQLKQVTYSLKMFPFDGVLLPFFNTQISEEGVFLPT
                     SSNSRALDMMFKELKRLGEGMKALR"
     gene            3678..4784
                     /locus_tag="PLG24_03320"
     CDS             3678..4784
                     /locus_tag="PLG24_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HMZ23946.1"
                     /translation="MSKYFSTWMMAIIALFSFFTNELIAQNSKKTENVVLILIDGYRW
                     QELFRGAERGLLADKRFNSEDSIARFKKYWDEDPLVRRKKLMPFTWEYIARHGQIYGN
                     RDYDNLVEVTNPYWISYPGRAELYSGFVDSSIQKNSHPNNSNINVLEFIHRQKGFEGS
                     VAAFVCWRPAGLALNKENAGFLINVPWEDVKGEKLTDAQKLANELQHYAPLVFGYGER
                     LDFAVYALASTYIMANHPRFTYIDLGDTDEYAHAGQYDKYLDDIFNIDRMIGNLWNSM
                     QADAFYKDKTTFIIVTDHGRGEGDQWTDHYHTVPHSGETWAMILGPDSKALGEVKTPG
                     RIYQNQFAATMARLLNLEYTANGRAGMPVESMMR"
     gene            complement(4945..5352)
                     /locus_tag="PLG24_03325"
     CDS             complement(4945..5352)
                     /locus_tag="PLG24_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HMZ23947.1"
                     /translation="MPGLDKTRVDKWLWAIRFFKSRSIASDACTGGKVKINGISAKPS
                     GLVKAGDIIECKKDHINYRLRVITVIDKRVGAPIAVTCYENITPEEELNKFESWFAGK
                     ARPEQRDKGTGRPTKKERRDIDDYKDSIFFEED"
     gene            complement(5352..6128)
                     /locus_tag="PLG24_03330"
     CDS             complement(5352..6128)
                     /locus_tag="PLG24_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HMZ23948.1"
                     /translation="MQKEIKLSKISTKAPKSMKKEDLITPTMELYERIGHLQHILYAE
                     KKHSVLIVFQGPDASGKDGATKNVFSRCSPQGVRVEAFKKPSEEEMNHDFLWRIHANT
                     PEKGFIQVFNRSHYEDILIQRVHKWIDEDRVHKRMAAINAFEELLQFDNNTLVLKFFM
                     HISKDNQLKQLKERLEEPEKNWKHNPNDWEERKLWNEYTKAYEYALNHSSIPWHIVPV
                     DNRWYRNYFIASVIVRELEALNMKLPVLDKNTLLADNSDD"
     gene            6350..7708
                     /locus_tag="PLG24_03335"
     CDS             6350..7708
                     /locus_tag="PLG24_03335"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HMZ23949.1"
                     /translation="MKFALPCVKNNLHFFLILALWVVSVKVSEAQSDFRIHRCGNTLM
                     DKIMEERHPGFLDNQNKAFSTAQNMPMPRNGDVLRIPVVFHVIYNTDKQNIPDEYIYN
                     QMEVINDAYRNRHGDTANTRPVFKHLAADAEIEFFLADIDPDGNPTNGITRTQTDIET
                     FGDLTLILGGFNLEHFEKMKHSNQGGHDAWPTNRYLNIWVADAGIKFLGLYFPALLGL
                     ATPPRYPTLPDNWPDEDFGDIVDGVFLQYQTIGNNNPYKSDLSDLVSAGRTAVHEIGH
                     YLGLRHINGDEECGSDGIDDTPVMDLSTQMANACPGTDVNTCGAGEPGDLPDMWENYM
                     DYSNDICQTLFTIGQVGHMRKVLTGQRDTLLNWALNTQRTNIEAMNLYPNPANSAIQI
                     GSIPLGGNMIISNSLGKVVMHFERLQEYRIDVSALPPGCYFITYLSEQTKKYNSRFVI
                     VR"
     gene            7949..8482
                     /locus_tag="PLG24_03340"
     CDS             7949..8482
                     /locus_tag="PLG24_03340"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HMZ23950.1"
                     /translation="MGDFMSQPMVQIVLRCIAVYLFVVLALRIFGKTELAQLSVVDLV
                     FILLISNSVQNAMVGPDTSLGGGLVAAFSLFTLNYGFKRLIAKNKKVSDLIQGKEILL
                     AYNGKVHMANLKKAGLTESELNAAIREHGVQDISGVDLAMLEVDGNISVISDDYKKRA
                     SLPSPHKHKHKWKGGIN"
     gene            complement(8545..10368)
                     /gene="typA"
                     /locus_tag="PLG24_03345"
     CDS             complement(8545..10368)
                     /gene="typA"
                     /locus_tag="PLG24_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HMZ23951.1"
                     /translation="MAQEIRNIAIIAHVDHGKTTTVDKILHQTNLFRDNQNPGELILD
                     NNDLERERGITIVSKNVAVNYKGVKINIIDTPGHADFGGEVERVLKMADGVLLLVDAF
                     EGPMPQTRFVTSKALSLGLKPIVVINKVDKENCRPDEIHEMIFDLFFALDATEEQLDF
                     PVVYGSSKHGWMSNDWKKPTTDFTALLDAIIEHIPSAPHNEGTLQMQVTSLDYSSFVG
                     RIAIGRVSRGSIKENQPIALMKRDGTVVRGRVKELHTFEGLGRKKTNEVGCGDICAVV
                     GLDGFDIGDTISDVENQEALPGIQIDEPTMSMLFTINNSPFFGKEGKYVTSRHIRERL
                     MKETEKNLALRVEDTESPDAMMVFGRGILHLSVLIETMRREGYELQVGQPRVIIKEID
                     GRKHEPIETLYVDVPAELSGKVIELATQRKAELTVMEPKGDLQHLEFNIPSRGIIGLR
                     NQVLTATAGQAVMNHRFKSFEPWVGDIPGRSMGVLVSMETGPCTAYALDKLQDRGTFF
                     VDPGEEIYEGQVVGEHIRENDLVINIVKAKQLTNFRASGKDDAARVTPPRKFSLEEAM
                     EYIQADEYLEVTPKSQRLRKIVLSESERRRLANPALAGMKM"
BASE COUNT         3073 a         2272 c         2396 g         3241 t
ORIGIN      
        1 aaattttcca ggtaggtaga tgattaataa tcgtctatta ccttttatcg caccctgaca
       61 acattagatc ctgaaattca caggctattt gcagtttgtt ttgacgtgat ggccgtaaaa
      121 gccataaata ctatagcgtc aaattattat ttttatggct acttgaattt ttatttcaat
      181 tcaacttatc caagggcgtt ttccccttac attttcatta tttatccttg aaatcgatcg
      241 ccggaccgtt agttgtgaaa ataattatat atattctttt gtatttcata gcaagttgtg
      301 aagaatgtat ttatctttgt agttaaatat ctacaagagt ggtcgagtta agtgaactat
      361 tgatagattt gattttcaag caaaaatcag caaccgtgcc tgaattgggt acgtttgagt
      421 ttgttgcaaa cccagccaga atacactttg gggaaaatat tatttatccg cccagtcaac
      481 aattggtttt ttccgatgga aaaatggata ccggagaagt tggatttacc gaatttctgg
      541 ttcaggagaa aggctttgat gaacatgaag ctcttatcag ggtcaaggcc tatgttcaca
      601 aaattaaagg gaaccttgac actctgggat attcttatct tcccggcctc ggcacgcttc
      661 accgatccga aaattcttca cttgaattta cacccggtga taagattaag gctatgaagc
      721 ctacccttgg gcttcctgaa atcaaggtgg agccaattgt tcgggaatat gtcgatcctg
      781 ataaggtggt cagggaaaca gaggtggctg gtccggtgaa tgatttaggc gatgacagca
      841 gatcatcgaa gtcttgggtt ctcccggtag tgcttgcctt tattctggta gggcttagcg
      901 taaccggtta ttacctgtat aaaatgcaat cagacaataa ggggcctcaa gaggcaaatc
      961 cggttgaaat accagcagat acagcaggtg ttgaagataa tcaggcaagt gccgtaccgt
     1021 ccgctgatga tgccggtctg aatggaatga ccgatgatga gggttcaacc tatcaaccgg
     1081 atgatattgt tactgacagt aatctttcgg aaaccaatac gcagggaatt tcaaatgcgg
     1141 aaacaaaaac tgaaacatct gcttcgtcat cctcaggtag tacaaagccg gttaaaaagg
     1201 tggaaaagaa ggctgaaaaa cctttggaag atacacccta tacaggaaaa gtctgtgcca
     1261 tcatagtagg agtcatgtca aagccggaaa atgccagaag attggccagg accataacga
     1321 acgcaggttt tcagccattt gcctatactt caaagggttt gacacgagtg ggggctactt
     1381 gcaattgcga tgatcaaagt attgattcaa cgctcaggca aatgaagaaa atcagcgctg
     1441 atgcctggct gtatgaatca gaataggcaa ggccttcaat gcttcagtga tcgaaggtac
     1501 tcacttctca ttttacttaa aaattccggt gttacaccaa tgtaggaagc catctgcttt
     1561 tgtgggatcc tgttgatcag ttcaggatat gtattgcaga aactgacaaa tctttccttt
     1621 gctgtcaggc ttagccggtc aataatacgt tgcctgttgg ctgcgagatt cttctccata
     1681 agtatcctga aataacgttc caacttagga acaagggaaa aggccttttc acggtcttcc
     1741 cgggttatca tcagaatggt actttcttca atagcatcaa tgtttagaga ggccggcatt
     1801 ccggtaacaa aactgtgaat gtcggtaatc caccaatcat ttggagcaaa ttgcaggatg
     1861 tgctcaaatc cgtttttgtc gacagaatag gcgcgaaggc aaccattcac tacaaatgat
     1921 aaatggtgat tggcctggcc ttcatgcaag atgaattgct tccttttaag cttcttttca
     1981 gtaaatagtt ctaaaatata agcttgctcc ttttctgaaa ggtcgatttt tcgccggata
     2041 ttatttacaa gtaattcatg attcattctg taaaattcaa taaaaattct caaaaaagca
     2101 atattggttg cataaatgac cggggaagct tttaatttct tttaattcag ccaaacgcac
     2161 cttgtaatgt ttaatggctc cggaacaata caaaattagt aggaggttat aagctattcg
     2221 cccgcttttc cttccaatca atccggcttt ctaattggtt tgacaagtat ccatatcaag
     2281 gataccatgc gcgctatact tatgcgatcg ggactatgga gaagttggag gacggacatt
     2341 tgtatttata ttttggtgcc aaacgatttt gtcctaatcc ggaccaaacg tgcattcacc
     2401 ccgcatgaat cagcaatttt gaaataaaat gcactgaata tttataaaaa caagcgggtt
     2461 tttcatagaa aatcgtgaag tttagtaatt ttacagtaaa accaataatt atggccacaa
     2521 acattttggg tcaggccttc gaaccggcca ctccgcccac ctccgacgaa aagacaatgg
     2581 cattgttatc tcatttgctc gttttagtag cctcctttgt cgcccctctg gtaatttatc
     2641 tattaaagaa agacgaatct tcctttgtag ccaataacgc caaagaatca ttgaatttcc
     2701 agattactat tgccattgct ataggcgtga gtgtcctttt gatgatggtc ctgatcgggt
     2761 ttcttttgat tttcgcagtt tcattagcta atctcgtata tgttatcata gcaaccatga
     2821 gagcttcgga agggaaagag tatcagtatc ctttctgcat tcgacttatc aaataaacat
     2881 tcattacttg aatctttttt cacaatggct gacttactcc ggtcaaccca atgactgttt
     2941 tatgtaatgg ttagagacat ctgtataccg gaactgaata ttttgtacaa aacggaaaaa
     3001 gcaataagga tatgttgaat ttgaagataa tccacgccag tacaagagaa gggcgccgcg
     3061 ggttgccggt ctcgaaatgg gtggtggatg cggcaagaaa ggaaacagat tttgaagttg
     3121 aattcatcga tctgaaggaa cttggacttc ccttcattga cgagcctgag catcctcgac
     3181 tcaagcaata cgtgcatcgg cacacagtgg aatggagtaa taaaatcgac catgccgatg
     3241 ccttcatctt tgtgacatgt gaatacaatc acggtatgcc ggccacactt aagaatgcac
     3301 tcgatttttt ggttcaggaa tgggccttta agccggttgc ctttgtcgga tatggcggta
     3361 tatctgcagg gacacgtgct atccagcaat tgaagcaggt cacatattca cttaagatgt
     3421 ttcccttcga tggggtgttg ttgccttttt tcaatacaca gatcagcgag gagggtgttt
     3481 ttttgccgac ctcttctaac agtagagcgc ttgatatgat gttcaaggaa ctaaaacgtc
     3541 ttggagaagg gatgaaggcg ttgcgttaga ttatccggag aaaacccttg cgttgtttga
     3601 aaattgacct tatatttgaa tgggttgtaa ttaggtaaca acgaatttta ttgaatgata
     3661 aaaattataa tgtcaagatg agtaaatatt tttctacatg gatgatggct atcatcgcac
     3721 ttttctcttt ttttacaaat gaactcatcg cccagaatag caaaaagaca gaaaatgtag
     3781 tgttgatcct gatcgatgga tatcgttggc aggaattgtt tagaggggca gagaggggtc
     3841 tgttggcgga taagagattt aacagcgagg attctatcgc ccgatttaag aaatactggg
     3901 acgaggatcc gttggtgcgc agaaaaaaat tgatgccctt tacttgggaa tacatcgcca
     3961 ggcatggcca gatatatggc aaccgggact atgacaattt ggttgaagtg actaacccat
     4021 actggatctc ctatccgggt cgcgctgaat tatacagtgg ttttgtcgat tcttccatcc
     4081 agaagaacag ccatcccaat aattcaaata tcaatgtact ggaattcatc catcggcaaa
     4141 aaggctttga gggaagtgta gcagcctttg tatgctggcg tccggccggc ctggccctga
     4201 ataaggagaa tgccggtttt ctcatcaatg tgccgtggga ggatgtcaag ggcgaaaagc
     4261 tgactgatgc ccaaaagctg gccaatgaat tacagcatta tgcaccactc gttttcggct
     4321 atggcgaacg actggatttt gccgtttatg cactggcttc cacctatatc atggccaacc
     4381 atccacgatt tacctatatc gatctgggtg atacagacga atatgcccat gcaggacagt
     4441 atgacaaata tctggatgac atattcaata tcgacagaat gattggcaat ctatggaatt
     4501 ccatgcaggc tgatgctttc tataaagata aaaccacttt catcatcgtc actgaccacg
     4561 gccgcggtga aggcgaccag tggaccgatc attatcatac ggttccgcat tccggtgaaa
     4621 cctgggcaat gatcctcgga cctgacagca aagcattagg ggaggtaaag actcccggca
     4681 ggatatacca gaatcagttt gcggcaacta tggcaaggct tctcaatctc gaatatacag
     4741 caaatggcag ggccggaatg ccggtggaaa gtatgatgag gtagcgtatt tactactttt
     4801 ggactactaa aaatgaacca taaaaggtaa aagaaatccg aataatcttt ttgtgcccta
     4861 cgtgtatttg tatcggaagg aaattcccga attcaatata taaaacacac gggtctatta
     4921 atcaggtaaa taattaacgg taatttaatc ttcttcaaag aagattgaat ccttataatc
     4981 gtctatatcg cgacgttctt tctttgtagg acgtcctgtt cctttgtccc tttgttccgg
     5041 tctggcctta ccggcaaacc agctttcaaa cttgttgagc tcttcttcag gtgttatatt
     5101 ctcgtaacag gtaacggcaa ttggggcgcc aacacgcttg tcaatgacgg taatcacccg
     5161 aaggcggtag ttgatatggt ctttcttaca ctctatgatg tctcctgctt taacaagccc
     5221 ggatggttta gcacttatgc cattgatctt cactttgcca ccggtacaag catctgaggc
     5281 tatgctgcgg cttttaaaaa accgaatcgc ccacagccat ttgtctaccc gcgtcttatc
     5341 aagccccggc atcaatcatc agaattatct gccaataaag tgtttttatc aagtacaggt
     5401 aatttcatgt tgagagcttc aagttcacga actatcacag aggcaataaa ataattgcga
     5461 taccatctgt tatcgacagg cacgatatgc catggaatgg agctatgatt taaagcgtat
     5521 tcataggctt tggtgtattc attccatagc ttccgctcct cccagtcatt gggattgtgt
     5581 ttccagtttt tttccggctc ctcaagcctt tccttaagct gtttgagttg attgtcttta
     5641 ctgatgtgca tgaagaactt aaggaccagt gtattgttgt caaattggag taattcttca
     5701 aatgcattga tggcagccat gcgcttatgt acccgatcct catcaatcca tttatgtacc
     5761 cgctgaatca gaatatcttc ataatgagag cggttgaaaa cctgaataaa tcccttttcc
     5821 ggtgtattgg catgaatcct ccaaagaaaa tcatgattca tttcctcttc actcggtttc
     5881 ttaaacgctt ctactcgcac accctgagga ctgcatcggg aaaacacatt cttggtcgca
     5941 ccatctttcc cgctggcgtc aggtccttga aaaacaatga gaacggagtg tttcttctcg
     6001 gcatataaaa tatgttgcaa atgaccaatg cgctcgtata gttccattgt cggggtaatg
     6061 agatcttcct ttttcatgga tttgggtgct tttgtagata ttttagagag tttgatttcc
     6121 ttctgcattt gaatactttg atttaagtaa aataaaagac aatcatcagt caaatatagc
     6181 tatctgtgaa aatacttcat aattcagcgt cagaaattat ccattgcctg ttgcagatcc
     6241 gccagccaga tccttcttct cctcattttt gtcttcaaaa taaattattg ggaatggtat
     6301 aaatcaacta atttggagcc aattattact tttaaaaaat attatactca tgaaatttgc
     6361 cttaccctgt gtgaaaaata atttgcactt ttttctgatt cttgccctgt gggttgtttc
     6421 ggtcaaagtg tctgaagccc agagtgattt caggatccac cgatgcggaa atacgctgat
     6481 ggacaaaatt atggaagaga ggcatcccgg tttcctggat aatcaaaata aagctttcag
     6541 cactgctcaa aatatgccga tgcctagaaa tggcgatgtt ctgcgaattc cggttgtgtt
     6601 tcacgtcatc tataatactg ataagcaaaa tataccggat gaatatattt acaatcaaat
     6661 ggaagttatc aatgatgctt atcgcaacag acatggcgat actgcgaata caagaccggt
     6721 attcaaacat ttggcggctg atgctgagat agagtttttt ctggccgata tcgacccgga
     6781 tggcaatcca accaacggca ttaccaggac ccaaacagat attgagacat ttggtgacct
     6841 cacccttatc ctgggcggtt tcaatctgga acattttgaa aaaatgaagc attctaatca
     6901 gggtgggcat gacgcctggc ctactaatcg ctatcttaac atatgggtgg ctgacgcagg
     6961 tatcaagttt ttgggtttgt actttccggc attgctagga ctggcaaccc cgccaagata
     7021 tcccacactg ccggacaact ggcccgatga agatttcggt gatatcgtcg atggagtgtt
     7081 cctgcaatat caaacaatcg gcaataacaa tccatacaaa tcggaccttt ccgatctggt
     7141 gtctgcagga cgtactgcag tacatgaaat aggccactat cttggtttac gtcatatcaa
     7201 tggcgatgaa gaatgcggtt cagacggaat tgatgatacg cctgtcatgg acctttccac
     7261 acaaatggct aatgcttgcc ccggtactga tgtaaatacc tgtggtgcag gagagccggg
     7321 ggatttaccg gatatgtggg aaaattacat ggattattcc aacgacattt gtcagactct
     7381 attcacaatc ggacaggtcg gccatatgag aaaagtgttg accgggcaac gcgatacact
     7441 cctcaattgg gcattgaata cacaaaggac aaatattgaa gccatgaacc tgtatcccaa
     7501 tcccgccaac agtgcgattc aaattggcag tattccactt ggtggaaata tgattatcag
     7561 caattccttg ggaaaagtgg tcatgcactt tgagcggtta caggaataca ggatcgatgt
     7621 atcagccctt cctccgggat gttatttcat aacatatttg tctgaacaga ctaagaaata
     7681 caacagccga ttcgttatag tgaggtaatg atagtacatt aacggatatg acaggtgttt
     7741 ggtattattg agctttaaag ctgggaccaa tctaccttcc ggccaatact aattttattt
     7801 ttaaccttgt ccgcgggaat gttttttcat tgattggata ttaagattaa ttacctttct
     7861 ggtatgattt ccggaataat aatactggtc attttatgtt aacttttttc ggaacaatga
     7921 ttattttgag gtaaaaattc agggaattat gggtgatttt atgtcacaac cgatggtgca
     7981 aattgtattg agatgtatag cggtatatct gtttgttgtg ttggcactgc gcatattcgg
     8041 gaagacagaa ttggcccaac tttctgttgt tgacctcgta ttcatcctgc ttatcagtaa
     8101 cagtgtgcag aatgcgatgg ttggaccgga cacaagcctg ggcggtggtt tggtggcagc
     8161 cttttctctc ttcacgctca attatggatt caagagactc atcgcaaaaa ataaaaaggt
     8221 cagtgatttg attcagggaa aggagatact tctggcttac aacggcaagg tgcatatggc
     8281 aaacctaaaa aaagccggtc tgactgaatc cgagttaaat gcagctatac gggaacatgg
     8341 cgtacaggac atctccgggg tagaccttgc gatgctggaa gtagatggaa atattagtgt
     8401 tatttcggat gattataaaa aaagggcttc ccttccttca cctcataagc acaagcataa
     8461 atggaagggc gggatcaatt gattactgcc cggcccccca atttatttta atgggtcgaa
     8521 tgttattaca aataattgtc aggtttacat cttcatgccg gccaatgcgg gattggccaa
     8581 acggcggcgt tcactctcag aaagaactat ttttctgagt cgctgacttt taggtgttac
     8641 ctccagatat tcatcagcct ggatatattc catagcctct tccagggaga atttacgggg
     8701 tggggtcacc ctggctgcat cgtccttacc ggaagccctg aagttggtca actgcttggc
     8761 tttcacgata ttgataacaa ggtcattttc gcggatatgc tctcccacaa cctgaccttc
     8821 gtagatttct tctcccggat ctacgaagaa ggtacctctg tcctgtagct tgtccaatgc
     8881 atatgccgta caaggtcctg tttccataga aaccagaaca cccatactcc tgcccgggat
     8941 gtcgccaacc catggttcga atgacttgaa cctgtgattc atcactgcct gaccggctgt
     9001 tgcagtcagg acctgattgc gcagaccgat aattccacgt gaaggtatat tgaattcaag
     9061 atgttgaaga tcaccttttg gctccattac ggtcaactct gcctttcgtt gtgtcgccag
     9121 ttctatcact tttccggaca gttctgcagg cacatctaca tagagggtct cgataggttc
     9181 atgcttcctg ccatcgatct ccttgatgat tacacgtggc tggcctactt gtagttcata
     9241 tccttcacga cgcatggtct cgatcagtac agagaggtgc aggataccac ggccgaatac
     9301 catcattgca tccggtgatt ctgtgtcctc cacacgaagg gctagatttt tttcggtttc
     9361 cttcatcagt cgttcgcgga tatgtcggga agtgacatat ttgccttcct tgccgaagaa
     9421 ggggctgttg ttaatggtga agagcatact catcgtaggc tcgtcaatct gaatacctgg
     9481 taatgcctcc tgattctcca catcactgat ggtatctcct atgtcaaagc catccaggcc
     9541 gacaacggca cagatgtcac cacaacctac ctcgttagtc ttttttcttc caagaccttc
     9601 gaatgtatgt aattccttca cacggcccct tacaacagtg ccgtctcttt tcatcaatgc
     9661 aatcggctga ttttccttta tgctgcccct gctcaccctg ccgatggcga tacgtcctac
     9721 gaaggaggaa tagtccagtg aggtaacttg catctgtaaa gtgccctcat tgtgaggggc
     9781 tgaaggtata tgctcgatga tggcatccag caatgcggtg aagtcggtag taggtttttt
     9841 ccagtcatta ctcatccagc catgctttga cgaaccatag acaacaggaa agtcaagttg
     9901 ttcttccgtt gcatcgaggg cgaagaacag gtcgaaaatc atttcgtgga tctcatccgg
     9961 tctgcaattt tccttgtcca ctttgttgat caccacgata ggctttagac ccagggaaag
    10021 agcctttgag gtgacaaatc ttgtctgagg catcggacct tcgaaggcat caaccagcaa
    10081 cagaacaccg tcggccatct taagaacacg ctcgacttca cctccgaaat ctgcgtgacc
    10141 gggagtgtca atgatgttga tctttactcc cttgtaattg acggcgacat tttttgatac
    10201 gatggtgatg ccacgttccc tttccaggtc gttgttgtcc agaatcaatt cacccggatt
    10261 ctgattgtcg cgaaacaggt tggtctgatg aagaatctta tcgacagtgg ttgtcttacc
    10321 gtggtctacg tgggcgatga tagcgatatt tctgatttcc tgagccattc aatattttgt
    10381 tttgaaaaaa gaggcgcaaa ggtacgtttt tttgtcttga aatatataca taaaatgcaa
    10441 atattcaatt cattatcaaa ttgttcggct tttttctttt caggaaaaat attatatgtt
    10501 aaaccgggtc gggcaaatgt ataagattgt gtaaccggtc aggcgcattg ccgataaata
    10561 gtgaacaata attcatagta atgattcaaa agcggaacat caacaaaatc caccgggaac
    10621 ccgaacagat tcagaaatga atatatcatc agaaacgctt attgctttga ttagaggttt
    10681 gcaaggattt tgtcggctga atctaattgg tcaaaaatcc ctataaattg gttgcctctg
    10741 atagcaatca tgtcaaattc agtgcatctc ggccatatag gtgattggtt ttaatggaga
    10801 agcatcaatt ttcaatttac gaattgatta aaaaaaggca aaatggttga agttttgagg
    10861 attgctgaga tattgaggca ggatacttcc catataccta atgatagaaa agggcaattc
    10921 tttatgcctc aaaaattgtc ggtcttaatc gagaaatgaa aggttatttt ttccatgccg
    10981 tt
//
LOCUS       DAOQQN010000055        10739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2221_length_10739_cov_5.049700, whole genome shotgun sequence.
ACCESSION   DAOQQN010000055 DAOQQN010000000
VERSION     DAOQQN010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10739
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2221_length_10739_cov_5.049700"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="PLG24_03350"
     CDS             <1..964
                     /locus_tag="PLG24_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HMZ23952.1"
                     /translation="LDLIKAPIREELEMFEKHFRDAMKSNVALLDRIMFYIVRRKGKQ
                     IRPIFIFLSAKAAGGCTDSTYNAASLIELLHTATLVHDDVVDESMERRGFFSINALWK
                     NKVAVLVGDYLLSQGLLLSVNNNEFELLRIVAKAVKDMSEGELLQIEKARRLDIQEEI
                     YFDIIRQKTASLIAAACEAGSTSAGVSKEVAKQYRLFGEKIGIAFQIKDDLFDYGSKD
                     VGKPLGIDIKEKKMTLPLIYALQNTDPSNKRRIINLIKNHSKKQKAVREVIEFVSQSG
                     GLQYAEQKMQEYCDSAISILRTLSTNDGAIALEKLIAYVTQREN"
     gene            complement(1094..1933)
                     /gene="panC"
                     /locus_tag="PLG24_03355"
     CDS             complement(1094..1933)
                     /gene="panC"
                     /locus_tag="PLG24_03355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HMZ23953.1"
                     /translation="MHIFTKKAGLKAYLDKERKKGKSIGFVPTMGALHEGHLSLIIGS
                     RKDCDVTVCSIFVNPTQFNNADDFRKYPRVVEADRDLLEKASCDVLFLPDVEEMYAGT
                     KPIRPDLDGLDTRLEGEFRPGHFDGVVQIVYLLLKAVKPDRLYMGLKDYQQQLIVGRM
                     IREMKLPVTLVAEPTMREKNGLAMSSRNMRLTPVQREEAAAIYAMLTHAAQSIKEGLD
                     IPEVLQKAESELADFDCKPEYIVLCDAETLLPLERYDPDRKAILLTAVWWGDVRLIDN
                     LVF"
     gene            complement(1950..2477)
                     /locus_tag="PLG24_03360"
     CDS             complement(1950..2477)
                     /locus_tag="PLG24_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HMZ23954.1"
                     /translation="MEKELVNRIANSPLITLDMEKHAPQWNYALFDIKDYLFHGLILK
                     EKDFRQALKELDWTQYEGKDLLVFCSNDAIIPMWAYMLIVAQASPYASNVIQEDEAAY
                     CSRIWKETIDTLDLSPYEGKKMIVKGCSDRPVPPSAYTTITGRLIKIAESVMFGEPCS
                     TVPVFKSKSVQARDK"
     gene            complement(2524..3087)
                     /gene="scpB"
                     /locus_tag="PLG24_03365"
     CDS             complement(2524..3087)
                     /gene="scpB"
                     /locus_tag="PLG24_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HMZ23955.1"
                     /translation="MNNLEQHIEVIIFTASEPVTAEFIGEMVSQIHGRDIGRDVVVGA
                     VEKINQRYESIHSVFKIFNIAGGYQLLTKKEYDPVIAHVQSNLSKKKLSTSALETLSV
                     IVYRQPVTKAVIESIRGVNSDYALQKLLEKGLVKIIGRSEDVGRPLLYAAGDAFLEHF
                     GLNDLNELPKLREFEEPEEAIGKPDTE"
     gene            complement(3084..4583)
                     /gene="hutH"
                     /locus_tag="PLG24_03370"
     CDS             complement(3084..4583)
                     /gene="hutH"
                     /locus_tag="PLG24_03370"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HMZ23956.1"
                     /translation="MSLSLEVSSPDISSDKLEAVFKGKATLVISSEALQRIDHCHQYL
                     QNRIHTTDDKLYGINTGFGSLCNVVVDDDNIGQLQINLIRSHACGAGNQVPPGISRII
                     LFLKIRSLSYGNSGVTTELVQRLIDMFNDDAIPVMYEQGSLGASGDLAPLAHMSLPLI
                     GEGLVHYRGSIISGGEYLELKGLTTYTLKAKEGLALINGTQFSLAYLQASYLLSEKLL
                     RAANTIAAMSIEGFDCRLDPFHPSIHAVRPHNGQVETATFFRDILSGSEHMQQPAVAV
                     QDPYSFRCIPQVHGATYNAMQHIGSVIEGERNSVTDNPNVFADEGLVISGGNFHAQPL
                     ALASDYLSIAVAELGSISERRLYLLVSGQRGLSPFLVTKPGLQSGFMIAQYTAASIVS
                     LNKQLASPSCVDSIVSSNGQEDHVSMAANAGVKLWQIVNNVATVLSIEWMAACQAVEM
                     KQYSISPVLKNLLDEYRKYVPFVDEDRVLHNDFIRTQEFFDDIKWFKKS"
     gene            complement(5030..6067)
                     /locus_tag="PLG24_03375"
     CDS             complement(5030..6067)
                     /locus_tag="PLG24_03375"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HMZ23957.1"
                     /translation="MIYKFSLIEKILLANDIIPHPFTDASSSVGLGFALGSSVKLKIV
                     DQLSMEFKDIKAIAKGADVSELGAELILDCLDALGYVRKQGNQYAFTKRGFKNLSPNS
                     PQNFRYFILFCDYLYKGYLDLDETIRLGKRAHSTMLEEMSDYEWELFSRAMIDISSTN
                     YKEVAGKIPIPKSALKMLDLGGSHGLYSIELCNRHPALKATILDLPPVKKYAEECITK
                     HNASQSVSFLPSDFMKDELPKNVDVILAFNIIHGLNPSENETLAQKVYNSLNSGGIYV
                     ILDQIKGIGGGSQMSKATTSYMALNLLHQAGGKTYSKNEVDAFTQKAGFNQTVLKKLN
                     APGFGLIICTK"
     gene            complement(6727..7167)
                     /locus_tag="PLG24_03380"
     CDS             complement(6727..7167)
                     /locus_tag="PLG24_03380"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ23958.1"
                     /translation="MATVNVYLTFNGNCKAAFDFYKSVFGGEFPYIGTFGEMPPGEDN
                     KPLSEEDANRIMHVTLPISKETCLMGSDTVGEWSASFKEGNNYSISINTESKDEADKI
                     FNALSAGGQVTMPMTHTFWGAYYGMFTDKFGINWMVNFDEQPQA"
     gene            complement(7185..7400)
                     /locus_tag="PLG24_03385"
     CDS             complement(7185..7400)
                     /locus_tag="PLG24_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23959.1"
                     /translation="MTSTDKIIVNGEKSTFKAEDDHHQYLETYQLEKNENSVTLTVKV
                     DTLEPWEAMMSTSFPQALKIIKELSEQ"
     gene            complement(7384..7755)
                     /locus_tag="PLG24_03390"
     CDS             complement(7384..7755)
                     /locus_tag="PLG24_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ23960.1"
                     /translation="MKLGAFSISLTVKDIHKSKEFYEKLGFTYKGGNIDQNWIVLKNE
                     NAVIGLFQGMFDKNILTFNPGWDGNAQNIEDFDDVRVIQDTLKNRGIDLTKEADPTTE
                     GPEYITLTDPDGNNILIDQHR"
     gene            complement(7761..8300)
                     /locus_tag="PLG24_03395"
     CDS             complement(7761..8300)
                     /locus_tag="PLG24_03395"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMZ23961.1"
                     /translation="MKNLKKIFAVIAIIIAIPLVVALFVKKDYVVEREIIINKPKAEV
                     FEYIKFLKNQDNYSKWNMMDPDMKKTYQGTDGTVGFISAWESKKENVGAGEQEIVNMI
                     DGERIDTKLRFKVPFESQNDAYMITEDMSNNQTKVKWGFKGAFPYPFNLMGLFMDMEK
                     AVGGDLEVGLQNLKNLLEK"
     gene            complement(8509..8916)
                     /locus_tag="PLG24_03400"
     CDS             complement(8509..8916)
                     /locus_tag="PLG24_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23962.1"
                     /translation="MKEIKIESDLQLICVRAKSFPQGIEESFKVLESTVPGRKGRECY
                     GIYEENDSGITYRAGMLKEADNEDVIYGLENYTIKKGKYLGVTIYNWKDNIPKIGQTF
                     DKMAEDQRVEKNSPSIEVYKGLKDVICMMKMKG"
     gene            complement(9005..9391)
                     /locus_tag="PLG24_03405"
     CDS             complement(9005..9391)
                     /locus_tag="PLG24_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23963.1"
                     /translation="MKLQIGEARVVKICSRKWWNSTQVEISEGEEYEFTAEGKWTDFG
                     RTKDADGYTCTCMQLFDKCKRAKQDRWFALIGSLDKNEDKYYLIGTKSCIPFNESGIL
                     YCFANDVKGFYWNNWGHVTLQLKRIK"
     gene            9972..>10739
                     /locus_tag="PLG24_03410"
     CDS             9972..>10739
                     /locus_tag="PLG24_03410"
                     /inference="COORDINATES: protein motif:HMM:NF016593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HMZ23964.1"
                     /translation="MEKIKLKIEKLKMLSDSVTPVGLYLKLRDKFPHSFLLESSDYHV
                     NSDNFSFLCIEPVSSFVLHKEKVVEEFFSKVEVKEIQKREDVLDLFRQYIGRFETDEQ
                     LPNNGFFGYTGFDAVRYFDTMKMSIKGDHDIPEMYYALFRFIIQIDHFKNEMTIIENR
                     LEHQSSALHTIEALILNNTHPAYDFRLVGETSSPVEDQQFMEWVQRGKKHCMLGDVFQ
                     IVLSKPFVQSFEGDDFNVYRALRSINPSPYLFYFDFNN"
BASE COUNT         2943 a         2412 c         2142 g         3242 t
ORIGIN      
        1 acttgatctt ataaaagctc ccatccgtga agagctggaa atgttcgaaa aacatttcag
       61 agatgccatg aagagcaatg tagctcttct ggacaggatc atgttttaca tcgtccgtcg
      121 aaaaggaaag cagatcagac cgattttcat atttctcagt gcaaaggcag caggtggatg
      181 tactgattcg acctacaatg cagcatctct gatcgaacta ctacacacgg caacgttggt
      241 gcacgatgat gtcgtagatg aaagcatgga acgccgtgga tttttcagta taaatgcctt
      301 atggaaaaac aaggttgctg ttcttgttgg cgattacctg ctatctcagg gactgttact
      361 ttcggtcaat aataatgaat tcgaacttct tcgaattgtg gccaaagcag tgaaggatat
      421 gagtgagggc gaattgctac agatagagaa ggcgcgccga ctggacatcc aggaggaaat
      481 ttactttgac atcatccgtc aaaaaactgc ttccctcatt gcagctgcct gcgaagccgg
      541 ctccacttcc gcaggcgtat caaaggaagt cgccaagcaa tatcgattat ttggtgaaaa
      601 aattggtatc gccttccaga tcaaggacga cttgttcgat tatggatcga aggatgtagg
      661 caagccatta ggtatcgaca tcaaagagaa gaagatgacc cttcctctta tctatgcgct
      721 gcagaatact gacccttcca acaaacgtcg catcatcaat cttatcaaga accacagtaa
      781 aaagcagaag gccgtccggg aagtcattga attcgtaagc caatccggtg gtctgcaata
      841 cgctgaacaa aagatgcagg aatattgcga ttccgctatt tcaattctca ggacactatc
      901 caccaatgat ggagccattg ccttagaaaa gctgatcgcg tatgtcaccc agcgggaaaa
      961 ttgatcgtta cccaaagccc gatgaaagga actcttgaag gctccgtgcc cgcctgactt
     1021 attggccctc aatctattta taatctaaag gccttcacct tcttccgatt cccttacacg
     1081 gatttacccc tcctcaaaat acaagattgt ctatcaaccg cacatcaccc caccaaaccg
     1141 ctgtgagcag gatcgccttc ctgtccggat cataacgctc caatggtaac aaagtctccg
     1201 catcacaaag gacgatgtat tccggcttac aatcgaaatc cgccaattca ctttctgctt
     1261 tctgcaatac ttcgggaata tccaggccct ctttgatgga ttgagccgca tgggtaagca
     1321 tcgcgtatat tgcggctgct tcttcccttt gaaccggtgt aagacgcata ttcctgctac
     1381 tcatggccag accgtttttt tcccgcattg taggttcagc gaccaacgtc actggcaact
     1441 tcatctcccg gatcatccgc ccgacgatga gctgctgctg ataatccttc agtcccatat
     1501 aaagtcggtc aggcttcacc gcttttagca ggagatagac gatctgcact acaccgtcga
     1561 aatgtcccgg acggaattct ccttccagcc ttgtgtccag gccatccagg tcaggacgaa
     1621 tgggcttggt acccgcgtac atctcctcta catccggtag gaaaagcaca tcacaggatg
     1681 ccttttccag caaatccctg tcggcctcga ctacccgcgg atatttcctg aaatcatcgg
     1741 cattgttgaa ctgggtagga ttgacgaaga tgctgcacac agtgacatca cagtcctttc
     1801 ggcttccgat aatcagcgaa agatggcctt cgtgcagggc tcccatagtc gggacaaagc
     1861 caatagactt accttttttg cgttccttat ccagataggc cttcagaccg gctttctttg
     1921 taaaaatatg catagcgaga ggtggatttt tatttatccc gggcctgaac agacttgctt
     1981 ttaaatacag gaacggtact gcatggttct ccgaacatca ccgattctgc aatcttaatc
     2041 agtctgccgg ttatcgtggt atatgcagaa ggtggcaccg gccggtcact gcaacccttg
     2101 acaatcatct tctttccctc atagggactc aggtccagag tatcaatcgt ctccttccaa
     2161 atccgggagc aataggcagc ttcatcctcc tgaatgacat tgcttgcata aggcgatgct
     2221 tgtgccacaa tcaacatata ggcccacatc gggatgatgg cgtcattgga gcagaacacc
     2281 agcaaatcct tgccctcata ctgcgtccag tccaactcct tcaaggcctg acgaaaatcc
     2341 ttctccttca gaataagccc gtgaaaaaga tagtctttaa tatcaaacaa ggcgtaattc
     2401 cactgcgggg catgtttttc catatccagc gtgataagcg gactgttggc aattcgattg
     2461 actaattcct tttccattcg ttcatgttgt gttttttaat aacagctatt caggtgaagt
     2521 ggtttactcc gtatccggct ttccgatggc ctcttcgggt tcttcaaatt cacggagctt
     2581 aggcagttcg ttcaggtcat tcaaaccgaa atgttccaga aaagcatcac cggcagcata
     2641 aaggagcggc ctgccaacat cctcactgcg cccgatgatc ttgaccagac ctttctccag
     2701 gagtttttgc aaggcatagt cgctatttac gccgcggatg ctttcgatca ctgccttggt
     2761 cacaggttgt cgatatacga tgacggaaag ggtctctaat gcggaagtgg aaagcttctt
     2821 cttgcttaag ttgctttgta catgggcgat gaccggatca tattcctttt tggtgagcaa
     2881 ttggtaacca ccggcgatgt taaatatttt aaaaaccgag tggatcgact cgtagcgctg
     2941 attgatcttt tcaaccgctc cgaccacaac atcgcgtccg atgtccctgc catgaatctg
     3001 gctgaccatc tccccgatga actcagcggt caccggctcc gatgctgtaa atatgattac
     3061 ttcaatatgt tgttcgaggt tgttcaagac ttcttaaacc attttatatc gtcaaaaaat
     3121 tcctgagtcc ggataaaatc gttgtgcaat acccgatctt cgtccacgaa aggtacgtat
     3181 ttccggtatt catccaacag gttttttaaa acaggactga tgctatactg cttcatctcc
     3241 actgcctgac aggctgccat ccattcaatc gacaggacgg tagcgacatt gttgacaatc
     3301 tgccaaagct ttacgcctgc attggccgcc atgctcacat ggtcttcctg accattgctc
     3361 gaaacgatac tgtccacgca ggatgggctt gccagctgtt tgttgaggct cacgatggat
     3421 gccgctgtat attgagcgat catgaaacca gactgcaagc ccggtttggt taccaaaaat
     3481 ggagaaaggc cgcgctgtcc ggacaccaat aggtaaagcc ggcgctcgga aatactaccc
     3541 agttcggcca cggctatcga cagataatcg cttgccagag ccaggggttg cgcatggaaa
     3601 ttgccacccg aaatgacaag accttcatca gcaaagacat tgggattatc ggtgacactg
     3661 ttgcgctccc cttcgatgac ggagccaatg tgctgcattg cattgtatgt ggcaccgtgc
     3721 acctgtggga tacagcggaa tgaataagga tcctgcaccg ctactgcagg ctgttgcatg
     3781 tgctcactac cgctaaggat atccctgaag aaagtcgctg tctccacctg accattgtgc
     3841 ggccgcacgg catggatgga agggtggaag gggtcaagcc ggcagtcaaa cccttctatc
     3901 gacatggcag caatggtatt cgctgccctg agcagctttt cggacaacag gtaggaggct
     3961 tgaagataag ccagggaaaa ctgggtacca ttgatcagcg cgaggccctc cttggctttc
     4021 agggtgtagg tggtcaggcc tttcagttcc agatattcac caccggagat gatactgcca
     4081 cgataatgga caagtccctc gccgatcagc ggtaatgaca tatgtgccaa aggagccaga
     4141 tcgccgcttg ctcccaaaga gccctgctcg tacatcaccg ggatggcgtc atcgttaaac
     4201 atatcgatta ggcgctgcac gagctcggtt gtcacgccag aattgccata cgatagactt
     4261 ctgatcttca ggaaaaggat gattctgcta atgcccggcg gcacctgatt gccggcgcca
     4321 caggcatgtg agcggatcag gttgatctgg agttgtccaa tgttgtcgtc gtccaccacc
     4381 acattgcaaa ggcttccgaa tcctgtattg atgccgtata atttatcgtc cgttgtatgg
     4441 attctgttct gcaggtattg atgacaatgg tcgatgcgct gtagtgcctc actactaata
     4501 acaagtgtgg ccttcccttt gaagacagcc tccagcttat cggagcttat gtcgggtgaa
     4561 gatacttcta aagatagact catgctgagc atttgtaaat tcgaaaatcc gggtgtgaag
     4621 tggtgcaaag ataggatttt tagatagaag ataatggagg acggaaaaat gtgaaatttt
     4681 gtgggtatga atgaatatcc ttgtaggcta tgttttcccc aatagctgat tctgaagaaa
     4741 tattggcata tcccttcact tccctgtaag gttaatgttt ccggtgtccc catacagggt
     4801 tggtccccac ctggtgattt cttaaacggt tgtttttttg cggtgaatat agattagtac
     4861 accgactcca attacaataa ttaaggcgtt gtcgaagccg tccatcccga tatcacaaaa
     4921 ccccggggca aaacgcaatt ccgcttacct tcatgcgttt taccccggat taatatgttc
     4981 aagatcgatg cctggcttgg gtgaagaaaa accgatctac cgttgaaaat tacttcgtgc
     5041 aaataattaa accgaacccc ggagcattta attttttgag tacagtctga ttaaatccgg
     5101 ctttttgagt gaaagcgtcc acctcgtttt tagaataagt ctttcctccc gcctgatgaa
     5161 gtaaattcaa agccatatat gacgttgtcg cttttgacat ctgcgaacca ccacctatac
     5221 cttttatttg gtcaagtatt acataaattc caccgctatt tagcgaatta tacacctttt
     5281 gagcaagcgt ttcattttct gatggattta gtccatgaat aatgttgaag gcaaggataa
     5341 catccacatt tttcggcaac tcgtccttca taaaatctga agggagaaat gaaacacttt
     5401 gactggcatt gtgctttgtt atgcactctt ctgcatattt ttttacagga ggaagatcaa
     5461 gaatagtagc ctttaaagca ggatgccgat tacaaagttc aatactgtat aagccatgcg
     5521 aaccaccaag gtctaacatc ttcaaagcac ttttgggtat cggaattttc cctgccacct
     5581 ctttataatt tgtacttgaa atatcgatca tggctcttga aaacagctcc cattcataat
     5641 cagacatttc ttccaacata gttgaatgag cccttttccc aagccgaatg gtttcatcta
     5701 aatcaagata ccctttgtac aggtaatcgc agaacaaaat gaaatatctg aaattttgag
     5761 gcgagttggg agataagttt ttaaaccctc ttttcgtaaa tgcatattga ttaccttgtt
     5821 tccggacata tcctaaagcg tccaaacaat cgagtatcag ttctgcacca agctcgctta
     5881 catcagctcc tttggcaatg gccttaatgt ctttaaactc catcgagagc tggtcgacaa
     5941 ttttcaattt tactgaacta cccaaggcaa atcccaaccc aactgaagag gatgcatcgg
     6001 taaatggatg cggtataatg tcattggcaa ggagtatttt ttcaattaaa gaaaatttgt
     6061 aaatcataat gaagacaatt tatagtttgg gacaaaaatg agaagtcgat attcgtcata
     6121 attgtatatt acggacaatt tgaaatcatc ggaaaaaatg tctctgtata tccttaccgc
     6181 atggatgata ttcgccaggt gtcaagacga ggtatgatgt gcatgtacgg atgaagggtg
     6241 attgatcgta aaaccccacc ggcaggacaa tttgagaaaa caataaatca taatggtgca
     6301 gcaggtccga cgaagaagaa tatcaggaat agataaaaaa tgtcccttgt tgatccccag
     6361 ccgcataata caaaaaaaca aaaggagagc tgaagtcagc agccggaaca aaatatagta
     6421 tttcgtctct gagattgaag ctcagcttaa ccgcacactt tgctaacaat tatttttgtc
     6481 tgtttgcgaa ttgtacaatt cagaagtcgc cagatacgct tcttctatgc ctgaaaattt
     6541 agatcatccg ggaaacaaat cttcaatagc ccgcattatt caatactgaa tccatagcat
     6601 tacaccggcc actccggcag ccacttttca gacaatggaa agccaggaca tcaagtcggg
     6661 gttataacat gccctaccga tttgatgcgg gtattttaga ccgtatttgt tgtgtctaac
     6721 ttttctttaa gcttgtggtt gctcatcaaa attgaccatc caattgatgc caaatttgtc
     6781 tgtaaacatt ccgtaatagg ctccccaaaa ggtatgagtc ataggcatag taacttgccc
     6841 ccctgctgaa agtgcgttga agattttgtc ggcctcatct ttactttcag tattgataga
     6901 gattgaataa ttattgccct ccttgaaact tgctgaccat tcaccgacgg tatcacttcc
     6961 catcaggcat gtttctttgc ttatgggtaa ggttacgtgc attattcgat ttgcatcttc
     7021 ctccgataag ggtttattgt cttctcccgg aggcatttcg ccaaaagtgc caatgtatgg
     7081 aaattctcct ccaaaaactg atttgtagaa gtcgaatgcc gctttgcaat tcccgttgaa
     7141 ggttaagtaa acatttactg ttgccatatt tgatctgttt taacttattg ttctgataat
     7201 tctttaatta tttttaatgc ttgtgggaag gaagtactca tcattgcttc ccagggttca
     7261 agcgtatcca cttttacggt aagtgttacc gaattttcat ttttttccag ttgataggtt
     7321 tccaaatatt ggtgatgatc atcttctgct ttgaacgtgc ttttctcccc atttacaatg
     7381 attttatctg tgctggtcaa ttaagatatt atttccgtca gggtcagtca gggtaatgta
     7441 ttccggacct tctgttgtag ggtctgcctc tttggttaaa tctatcccac gatttttcaa
     7501 agtatcctgg atcactctta catcgtcaaa atcttcaata ttttgtgcat taccatccca
     7561 tcccggatta aatgtaagga tatttttatc aaacattcct tgaaacaatc caattactgc
     7621 gttttcgttt ttcaaaacta tccaattctg gtctatgttc ccgcctttat aggtaaaccc
     7681 tagtttctca taaaattctt tcgatttatg aatatccttt acagtcaggc taattgaaaa
     7741 tgctccaagt ttcatagcta ttatttttct aaaagatttt tcaaattttg taatcctact
     7801 tccaaatctc cgcccactgc tttttccata tccataaaca gccccattaa attaaaagga
     7861 tagggaaatg cgcccttaaa accccatttt accttagtct ggttgttact catgtcttca
     7921 gtaatcatat acgcatcgtt ttgagactca aaaggcactt taaaacgaag tttcgtatct
     7981 attctttcac cgtcaatcat gttaacaatt tcttgttctc cggcacctac attttccttt
     8041 ttactttccc aggctgaaat aaagccaacc gttccgtctg tcccttgata agttttcttc
     8101 atatcagggt ccatcatatt ccatttactg taattgtcct ggttcttgag aaacttaata
     8161 tattcaaaaa cctcggcttt gggtttatta ataataattt cccgttctac cacatagtct
     8221 ttttttacaa aaagtgcaac aactaaggga atggcaataa ttattgctat tacagcaaaa
     8281 atttttttca aatttttcat atctcgtaca ttttaaattg ttagtttttt aaaactattt
     8341 taatatatca gccatttccg taattcaaca tttgcgataa cgattcgtca aatatttagg
     8401 gaacagcgtg tcctaactaa tgttgataaa atttttccgc ctgacctgtc actaaagtcc
     8461 aatggcagcc actcgccctc ttgtggattt atttgccgga cccgggttct aacctttcat
     8521 cttcatcata caaatcacat ctttcaatcc tttatagacc tctattgatg ggctgttttt
     8581 ttccactcgt tgatcttctg ccattttatc aaaggtttgt ccaattttag gaatattgtc
     8641 tttccaatta taaattgtta ctcccaaata tttacctttt ttgatcgtat aattttcaag
     8701 cccgtaaata acgtcttcat tgtcggcttc tttcaacatt cctgcccgat acgttattcc
     8761 tgaatcattt tcctcataaa tgccataaca ttcccttcct tttctacccg gaaccgtact
     8821 ttccagaact ttaaaggact cttcaatgcc ttgtgggaat gattttgcac gaacacagat
     8881 taattgcagg tccgattcaa tttttatttc tttcataatg tgacagtatc atttaacctt
     8941 attaatttga ccagacttgt tattgatgca ctgttcttga ttgtgccttg tctgaaaagt
     9001 tgacttactt tattcgttta agctgtagcg ttacatgacc ccaattattc cagtaaaacc
     9061 ctttaacatc attggcaaaa caatagagta taccactttc attaaatgga atgcaacttt
     9121 tcgttccaat gagataatac ttatcctcat ttttatccaa agaaccaatc aaagcaaacc
     9181 atcgatcttg ctttgctctt ttacacttgt caaatagttg catacaagta catgtataac
     9241 cgtcagcatc ctttgttctt ccaaaatccg tccatttgcc ctcagctgta aattcatatt
     9301 cttccccttc tgaaatttct acttgtgttg aattccacca tttcctcgaa catatcttga
     9361 caactctggc ttcacctatc tgcagtttca ttttttgtta tgcttacgtt tgataaattt
     9421 ctctgccaaa gtttagtgaa tgggatatac tgaactaaac tttctccggt tccacctccc
     9481 ttcacgtaat ctcttgtaaa tatacataaa gttttcgtcc ttgcaaattt atcaaaaaac
     9541 aggtttataa ggctttccgg gaggaataat ttaccggaag gtggaggggg tttggagagg
     9601 tatcagtgcc ggcctaccaa tcattcctta agctatccat caatccatgg aatgccggga
     9661 ctattaaccg ggagtcaaag ggcagctcta catcgttggg ggtattgaat ccgttgtgtc
     9721 atcatccatg tattgcagct tgcagtgcat ggggtcacat agcggtttta attaaagtca
     9781 aaaaaaaatt gatacaagta ataaaatgtt ttttactttt gtccatccaa ttgaaggaaa
     9841 ataatgagta ttgtaaattt aatatggtgg ctggattatg aacgaataag gtcgtaatct
     9901 gtaactgtgt ttaaatttca aaaaaggttt gtcgattacg gcaaaccttt ttttttgacc
     9961 aaattttgac gatggaaaaa ataaaattga aaattgaaaa attgaaaatg ctctccgaca
    10021 gcgtgactcc tgttggattg tatcttaaat tgagggataa atttccgcat tcttttttgc
    10081 tggaaagttc tgattatcat gtcaatagtg ataatttttc atttctctgt atcgagccgg
    10141 tttcatcatt tgtccttcat aaggaaaaag ttgttgaaga atttttcagc aaggtcgagg
    10201 taaaggaaat tcagaaaaga gaagatgtcc tcgatttgtt ccggcaatat atcggaagat
    10261 tcgaaaccga tgaacaactt ccaaataatg gattcttcgg ctatacggga tttgatgctg
    10321 tcaggtattt tgacacgatg aagatgagca taaaaggcga ccacgacatc cccgaaatgt
    10381 actatgcgct gttcagattt attatccaga ttgatcattt taaaaatgag atgactataa
    10441 tagaaaatag gctggaacac caatcttcgg cattacatac cattgaggca ttgatactca
    10501 ataatacaca tccggcttat gatttccggt tggttggcga aacgtcatca cccgtagagg
    10561 atcagcaatt tatggaatgg gtacagcggg gaaaaaaaca ttgtatgctg ggggatgtct
    10621 ttcagattgt cctgtccaag ccgtttgtcc aatcttttga aggagatgat tttaatgttt
    10681 acagggcttt gcgctctatc aatccatcgc cctatttgtt ttattttgat ttcaataat
//
LOCUS       DAOQQN010000056        10708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2233_length_10708_cov_5.926875, whole genome shotgun sequence.
ACCESSION   DAOQQN010000056 DAOQQN010000000
VERSION     DAOQQN010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10708
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2233_length_10708_cov_5.926875"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PLG24_03415"
     CDS             complement(<1..804)
                     /locus_tag="PLG24_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23965.1"
                     /translation="MRLLNHFTLTLAFCFSCINLFSQNIYLAYDGMSGAAGSPLHGGS
                     GGTGWADTWTVQNDNTDGYLYYGTGLTFAGLQQSDMAITGGESYLTSGRALDCNPAGP
                     FASYISENNGIGSNTGTTLWVSALVQKSQNNDQNISYTLHGSSIAWCDNCSQDNRVSF
                     GYFGNESNVGGERRWSLKIGNDVFTSTVPVIIGSTALLVARIDFNEGNTVINMYINPS
                     ELGYAGPPSMPSISQTTNMLLRVRSLSAYLGDVAGNGLMDEFRFASSYAV"
     gene            complement(991..2079)
                     /locus_tag="PLG24_03420"
     CDS             complement(991..2079)
                     /locus_tag="PLG24_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HMZ23966.1"
                     /translation="MGFLFIDMKPADYTSLNLNGTSLEGPEILEFCNAQSEEHYTQLS
                     QFLSQWLDDREDLEVKTSGSTGTPKAICIPKARMLQSAAATARFFGFQPGETALLSLP
                     VSHIAGKMMVVRALYSHLNLKYIRPSHHPITELGTQDKINFAPFTPMQLSEANDTGNI
                     DTILLGGGPVSPDLELKLQMWQAAIYHGFGMTETLSHIALRRVNGPDASGVYKALPGV
                     ELTVDERSCLVIRAPFVEGPVVTNDIVDLQGNDAFVWRGRIDHVINSGGIKLFPEEIE
                     KKLNTCIDRDYFIAGIPDPIFGEKICLFIEGEAWSYEQQDKLSNIMDQCLTKYERPKK
                     LYFVLNFSRTLSGKIQRKTTIEYVLQNR"
     gene            complement(2055..3065)
                     /locus_tag="PLG24_03425"
     CDS             complement(2055..3065)
                     /locus_tag="PLG24_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HMZ23967.1"
                     /translation="MFRRPGGTSRGVLHTKDTYIVTMRKDGKIALGECNLFKGLSYDD
                     RPDYEDKLQEICRRIPEEGENLLSELSNWPSIRFGMEMMLLDWQNDCKQEIFPQVIGD
                     EGFSIPINGLIWMGPHDYMMEQVTTKLSEGYTSIKLKVGAIDFQSELELIRYIRREFS
                     ADEVEIRLDANGAFTPTEAKEKLDRLAKYDIHYMEQPIRAGQWQETARLVEDSPIKIA
                     LDEELIGINTANRQRELIETIRPAILILKPALLGGFKACDDWTALLEDIGGYRIITSA
                     LESNIGLNAIAQYAALTASPHAYGLGTGMLYSNNFPSPYHVDAQGLHYRRDQTWDFSS
                     LT"
     gene            4153..4878
                     /gene="pgl"
                     /locus_tag="PLG24_03430"
     CDS             4153..4878
                     /gene="pgl"
                     /locus_tag="PLG24_03430"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HMZ23968.1"
                     /translation="MNIEVKSTAEFNDFAARRILSIIEATVDHQGHCTVALSGGNTPK
                     EVYAHIANLYPEYDINWRRVFFFWGDERMVSIEDANNNAHMAYEYMLDALPVPDFNIF
                     PFRTELGAEAAAADMEEKIRNHFDVLTINAFDLTLLGLGEDGHTLSLFPHSYAMKVMG
                     QWVYGYYVNERMGHRVTFLPVPINNSMNTLFLVKGENKAEVVKHVLFGRKDIDLYPAQ
                     AIRPNNGKLEWILDESAAAKVKK"
     gene            5415..5678
                     /locus_tag="PLG24_03435"
     CDS             5415..5678
                     /locus_tag="PLG24_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23969.1"
                     /translation="MKRFFIYFIELTALPVIVIGTGTGLSSYYTATYGVMSRASHGTS
                     VCIQLIGQERQCSITENAQGQVFSVYRKAVNPLIDLSHHSIIT"
     gene            5682..6704
                     /locus_tag="PLG24_03440"
     CDS             5682..6704
                     /locus_tag="PLG24_03440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMZ23970.1"
                     /translation="MADKIRYLSCFVLTYWVLRLIFSSVFIRAGEGLVEGVANRNISG
                     WGDPSLTLQAATPFYGSLLFYDILLIITIISIYLGVWLRSRYLGKVKARLKQNMERQT
                     EVLHHNIIRLEQSERRLLESNRMKDEITAMILHDLRSPIRFVKLMAAYLEKNFDTMPT
                     KQLEQRISALKSSTSALNEFTEQFFTWAASQHQDFKVNKVFFDLKDLFLEIKSLYQDI
                     LVINENEIVIEPCYIIALTDRQILSTIIRNIVDNSNKIMKKGRIHISGNVEYGGVNIF
                     VTDNGPGFEKGKLQKFLGNGQLATPGGHGSIILRNLIKIIEGRLEVYSEEGIGTIFKI
                     TLPSQG"
     gene            complement(6676..7314)
                     /locus_tag="PLG24_03445"
     CDS             complement(6676..7314)
                     /locus_tag="PLG24_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ23971.1"
                     /translation="MLRIIIADDHFAVRVGLEILVRDTVDEDVSIDFASDGLELLSKL
                     KKSYYDVLLTDLNMPGIEGLPMLQKIREISPSLRILVVSVNSEEFYAIKAHELGAYGF
                     VHKASSDEELKEAIAAVCQGEKYVSEHQQRLINDQNPNPFVNLSPRELEVLNLLFKGY
                     GVLEISNELNIGQTTASTFKSRILKKTSTHSVVELIKLAEQYGFIPDSAKLS"
     gene            7764..8486
                     /locus_tag="PLG24_03450"
     CDS             7764..8486
                     /locus_tag="PLG24_03450"
                     /inference="COORDINATES: protein motif:HMM:NF039190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5672 family protein"
                     /protein_id="HMZ23972.1"
                     /translation="MPYPHQVVIVIPVYKPFPDPEEIRALRRAVSVFSRHPIRLLGPD
                     DIDYSVYLDAGIDRCSIIQMPGHHFRGIKAYNRLLCSKGFYAIFDVFEYILIFQTDAW
                     VFRDELSEWCERGYDYIGSPWLWRPERVKNSQILDLWPLMKGRVGNGGVSLRKIKVMK
                     KYALLARIFFALTAKNEDFIWLLVSLLPGVRIKKPNADEALKFCIEMEPEEALNRTGS
                     LPFTVHAYMRYGADYWKAYMPD"
     gene            complement(8940..9170)
                     /locus_tag="PLG24_03455"
     CDS             complement(8940..9170)
                     /locus_tag="PLG24_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23973.1"
                     /translation="MILLYQNMIVKLCYDDYSFLPDRRYNNLSLLSGYLLRLYAKMAS
                     RVDFLGSKRTSGKSCFNVIGELGLSGVSLTKK"
     gene            complement(9257..10171)
                     /locus_tag="PLG24_03460"
     CDS             complement(9257..10171)
                     /locus_tag="PLG24_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HMZ23974.1"
                     /translation="MKLVTVGTVAFDDIETPRGKAGKVIGGAATYISRSASFFVENQG
                     IVSVIGGDFPQQELDEMTSLGIDISGIEIRKDEKSFFWAGRYHDNMNQRDTLITDLNV
                     LANFQPKLPQSFRTADYLMLGNLTPEVQMNVLQQMTRRPKLVVLDTMNFWMDVAWDSL
                     QKVLRMVDVITINDEEARQLSGEYSLAAAAAKIYRMGPKYLIIKKGEHGVMLFREGRM
                     FSFPALPVSEVIDPTGAGDTFAGGFIGYLARTEDLSFENMKRAIVYASVMASFCVEDF
                     SLAKLKSINHLDIAMRVDIFKDMTHFVM"
     gene            10520..10593
                     /locus_tag="PLG24_03465"
     tRNA            10520..10593
                     /locus_tag="PLG24_03465"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10554..10556,aa:Asn,seq:gtt)
BASE COUNT         3080 a         2086 c         2345 g         3197 t
ORIGIN      
        1 cacggcgtag gaagatgcga atctgaattc atccatcagg ccattcccgg caacatcgcc
       61 cagataagcg gaaagtgaac gaaccctgag cagcatgttg gttgtttggg aaatggaagg
      121 catggatgga ggtccggcat aacccagttc tgaagggttg atatacatat tgatgaccgt
      181 gttgccttca ttaaagtcaa tacgtgcaac aagtaaggct gttgagccaa tgattacagg
      241 gactgtagaa gtaaacacat cattacctat cttcaacgac catcttcttt caccacctac
      301 attggattcg ttgccaaaat atccaaaact aactctattg tcttgactac aattgtcgca
      361 ccacgctatg gaagacccat gcagcgtata ggaaatattt tggtcattat tctgactttt
      421 ttggaccaag gcacttaccc aaagtgtcgt accggtgttg ctgccgattc cgttattttc
      481 tgaaatataa gaggcaaaag gacctgcggg gttgcaatct agggctctcc cactagtcag
      541 ataactttca ccacccgtaa ttgccatatc ggattgttgg agtccggcaa aagtgagtcc
      601 ggttccataa taaaggtagc cgtcggtatt gtcgttctgg acagtccagg tatctgccca
      661 tccggtccca ccggagccac catgcagcgg agatcctgca gcgccagaca tgccatcata
      721 ggccaggtag atgttttgag aaaatagatt aatgcaggaa aagcagaatg ccagagtcaa
      781 ggtaaaatga tttaaaaggc gcattgtgtt aaagatttag gtagtaacaa tgattattca
      841 cggatgatga gcaaaaagga tcgattaatc cgggtcaata ttaagtatat ttactaaaat
      901 atggaaattt tcaaattact tttaatcaaa ttaaaaattt taacatcaaa ctgaacttcc
      961 ggtaaacaaa gcctcggtgt aatacaataa ttatcggttt tgtaaaacat attcaattgt
     1021 tgtcttcctc tgaattttac cggataatgt cctggaaaaa ttaagtacga aatataactt
     1081 cttgggacgt tcatattttg tcagacattg atccattata ttggacaatt tgtcttgttg
     1141 ttcgtaactc caggcctctc cttcaatgaa gaggcaaatt ttttctccga aaataggatc
     1201 cggaatgccg gcaatgaaat aatctctatc gatgcaggta tttaattttt tctcgatctc
     1261 ctccggaaac aacttgattc ctccactatt gatgacatgg tcaatcctac cccgccatac
     1321 aaaggcatca ttaccctgta aatcgactat atcattggtc acgacggggc cctccacgaa
     1381 tggtgccctg atgaccagac aacttctttc gtccacggtt aattccactc caggcaatgc
     1441 tttatacacg ccggaagcat ccggcccgtt aacccttctt agggcgatat ggctcaatgt
     1501 ttctgtcatg ccgaatccgt ggtatatcgc cgcttgccac atctgaagct ttagttccag
     1561 gtccggactg acaggcccgc cacccaatag gatggtatcg atattccctg tatcattggc
     1621 ttcgctgagt tgcataggtg taaatggtgc aaaattgatc ttgtcctgag tgcccaattc
     1681 tgtaatcgga tgatgagagg ggcgaatata ttttagattc agatggctgt atagcgctct
     1741 aaccaccatc attttgccgg ctatatggct gactggcaga ctcaacaagg ctgtctctcc
     1801 cggttgaaaa ccgaaaaaac gagctgttgc agcagcactt tgaagcattc tcgcttttgg
     1861 gatacagatt gctttaggag ttccggtgga gccacttgtt ttgacttcca aatcctccct
     1921 atcatccagc cattgggata agaattggct aagttgggtg tagtgttcct cactttgtgc
     1981 attacaaaat tcgaggattt ccgggccttc gagtgaggtt ccgttcaagt tgagagaggt
     2041 ataatcagca ggcttcatgt caatgaagag aaatcccatg tttggtcacg acgatagtgg
     2101 agcccttgtg catcgacatg atagggtgac ggaaaattat tggaatagag catgcccgtg
     2161 cctaatccat aagcatgtgg cgatgccgtc aacgccgcat actgagcgat tgcgttgagg
     2221 cctatattac tttccaatgc agaggtgatg atgcgataac ctcctatatc ttccaacagt
     2281 gctgtccagt catcacatgc cttaaagccg ccaagaagtg caggcttaag aatcaggatt
     2341 gcgggtcgga tggtctctat caattctctt tgtcgattgg ctgtattgat gccgattagc
     2401 tcttcatcca gcgcaatctt aatcggggaa tcctccacca ggcgtgctgt ctcctgccat
     2461 tggcctgccc ggattggttg ttccatataa tggatatcat atttggccag tctgtctagt
     2521 ttctctttag cttctgtagg agtaaaggcc ccattggcat cgagacgtat ttccacttca
     2581 tctgccgaaa attcgcgccg gatatacctg ataagttcca attctgattg aaaatcgata
     2641 gcccctacct tgagtttgat tgaagtatag ccttctgaca atttggttgt aacctgttcc
     2701 atcatgtagt catgtggtcc catccaaatc agtccgttga tcggaattga gaatccttcg
     2761 tcgccaatta cctgtggaaa aatttcctgt ttgcaatcat tttgccaatc cagcaacatc
     2821 atctccatac caaatctaat cgatggccaa ttgctcaatt cagacagaag attttctcct
     2881 tcctctggta ttcgtctaca tatttcctgt agcttatctt cataatccgg cctgtcgtca
     2941 taggaaaggc ctttgaacag attgcattca cccaaggcga ttttcccgtc tttcctcatt
     3001 gtaacgatat aggtatcctt ggtatgcagc acacctcggg aggtgcctcc aggcctcctg
     3061 aacaacaatg agtatttttg aaaactgcaa tccatcccgt tgaatgacaa tataaagagc
     3121 aaatataatt aattgctgtc ttagcatatt ttcaattata aaataaaggg tcaaattgct
     3181 gcttacaggg cgctatccac ttttacttta aggtataatg gtaattatat cactgtttct
     3241 tattgtccaa ttcccggaca gagaactggt atagaatacg gtttaggtgc tatattttgt
     3301 tttttcttct ttattgtagc gggtttgttc tgaagaggaa ttaaaatgga atgtctatcc
     3361 tgttttttga tggttggaga gggtttgggt tttaagccat aacggcaaaa gctggcacta
     3421 tccagccttg ttccctattt tataaatgtt atcaaatcaa aaaactttca tttgcattga
     3481 gaacaactgg caaagaaatg taggccgatt atttttgaga aaagtgaggt tcttttgttt
     3541 catttggaca atgggcagtg ttcgtgaggt ttacatgacc aatggatatg tttggaagag
     3601 caaggttaaa gaaaagacaa tgagcaaatg ataattagtc atttggggct aacagtgatg
     3661 taatcagcct tgtgtgtagg gttaaggctt tggaatcaaa gaactcacac cagaccggat
     3721 acactggctg catcgccaat ttattgagag gaagaaccaa gaagaatgag tggtactcat
     3781 tagaaagagc aggtacgcct tcaaaaataa ttaaattagt cttagtagag gattacgtgc
     3841 aaaatttgtc ggttggctgg ttggtatagt cgatgatcga atcattcatt tcgaaaaatt
     3901 tcaaaaaaag ttctgtttgt atagaacatt ttttcattct aaatgtaatt gttagcacaa
     3961 tgtaaggcta tctttcatcg ttattctcaa atggtaaatt gtgttaggac gatgcgataa
     4021 tagtgacttt ggcaggtttc attagcgctg agttttcata tggtgagtgg aaaaccttct
     4081 aaggagcatc gccagataac ggtcaacggc ataattttcc acaaaacgaa tttatttaaa
     4141 aacaaacgag tcatgaatat tgaagtaaaa tctactgctg aattcaacga tttcgcagca
     4201 cggcgaattc tttccattat cgaagcaact gtagatcacc aggggcattg tactgtcgcg
     4261 ttgtccggag gaaacacacc aaaggaagtc tatgcccata tagccaatct ttatccggaa
     4321 tacgatatca actggaggcg ggttttcttt ttttggggag atgaaaggat ggttagtatt
     4381 gaggatgcca ataacaatgc ccatatggcg tatgagtata tgctggatgc cttgcctgtt
     4441 cctgatttta acatatttcc ttttcggaca gaattggggg ccgaagcagc cgccgcagat
     4501 atggaggaaa aaatcagaaa tcattttgat gtactcacca taaatgcgtt cgatcttacc
     4561 ttgttaggtc ttggagagga tggccataca ttgtcattat ttccgcattc atatgcgatg
     4621 aaagttatgg ggcaatgggt ctatggatat tatgtcaatg agagaatggg acaccgggtt
     4681 actttcctgc ctgtaccaat caacaattcg atgaatactc tttttctggt caaaggcgaa
     4741 aataaagccg aagttgtgaa gcatgtcctg ttcggccgaa aagatattga cttgtatcct
     4801 gctcaggcaa tacgaccaaa caatggcaaa ctggaatgga ttctggatga atccgcggcg
     4861 gccaaggtaa agaaataata atttgttggt aaaaactcct gcaccgattg tcaagagcag
     4921 gtaggtgatt cgaattgcat tggtgatttc cgggtgcggt tgtactattt taaaatgaag
     4981 gtccatcaca agcatgatgt gaattggagg aaggatatta ttggtttgga aatatctttt
     5041 tgcctccggt gacgcaccat cagatattta atggaaaaag agattaaaaa tagcctttaa
     5101 gggttttggg gtcaaccgcc attcgatccg ggaatattta tcaaaactaa caggggacag
     5161 gggtttggcc aatattccaa aaaaaaaccg ctcctaaata tggggaaacg gagcggtttt
     5221 aagtattaga gtatctcaac cggggttgag caaaattttt cgtgcatgaa tctattgaca
     5281 atcaatgtct tcgaaaatga ttattcaaag ataggcattg ttgtaaaaat acatatgtag
     5341 tattttaact acaaaaagtc tcagattgct gtcaatatat aacggatagg ttgtaaattg
     5401 aggcgatttt gtgcatgaaa cgatttttca tttatttcat tgagctcacg gctctccctg
     5461 tcattgttat aggtacaggt acagggctct cttcgtatta tacggcaact tatggagtca
     5521 tgagtagggc gagccatggc acatcagtgt gcattcaact tatcggtcag gaaagacaat
     5581 gctcaattac agaaaatgcg caaggacagg tattttccgt ttacaggaaa gcggtcaacc
     5641 ctttgattga ccttagtcat cattccataa tcacctgaag gatggcagac aagatcagat
     5701 acctttcatg ttttgtgttg acctactggg ttcttcggtt aattttttca tctgtattta
     5761 tcagggcagg ggaaggtttg gtagagggtg tggccaaccg gaacatttcc ggttgggggg
     5821 atcccagcct gacattgcaa gcagcaactc cattctatgg ttctttgctt ttctatgata
     5881 tattattgat catcactata atatcaatct atctgggggt atggctacgc tcgaggtacc
     5941 tcgggaaggt gaaagctcgt ctgaagcaaa acatggaacg gcagacagag gttttacatc
     6001 acaatatcat taggcttgaa caatccgaaa gaaggctcct ggaaagcaac aggatgaagg
     6061 atgagattac tgccatgatt ctgcatgacc tgagatcccc tatccgattt gttaagttga
     6121 tggcggcgta cctggagaaa aattttgaca ctatgcctac taagcagctt gagcaaagga
     6181 tttccgcctt gaaaagctcc acatctgctt tgaatgagtt tacggaacaa ttctttacct
     6241 gggcggcaag ccagcatcag gattttaaag tcaacaaagt attttttgat ttgaaggatt
     6301 tatttttgga gatcaaatct ctctatcagg atattcttgt tatcaacgaa aacgaaatag
     6361 tcattgagcc atgttatatc atagctttga ctgacaggca gattctttct accatcatcc
     6421 ggaacatcgt ggacaattcc aacaagatta tgaagaaagg caggattcac atcagtggta
     6481 atgttgaata tggcggggtc aacatatttg ttaccgataa tgggcctggc tttgaaaagg
     6541 gaaaacttca aaaatttctg ggcaatggcc aactggccac gcccggaggt catggtagta
     6601 tcattcttcg caacctgatc aagatcattg aaggaaggct tgaagtgtac agtgaagaag
     6661 gtatagggac aattttcaag ataactttgc cgagtcaggg ataaaaccgt actgttcagc
     6721 cagcttgatt agctctacta ccgaatgggt tgatgttttt ttgagaatcc gggatttgaa
     6781 tgtgcttgca gttgtctgac ctatattcag ctcattggaa atctcaagca caccataacc
     6841 cttgaataac agattcagga cttccagctc gcgcggtgac agattgacaa atggattggg
     6901 attctggtca ttaatcagtc tttgctgatg ttccgaaaca tatttttcgc cctggcacac
     6961 tgctgcgatt gcttccttga gttcttcatc actggatgcc ttatgtacaa agccatatgc
     7021 ccccagttcg tgtgccttta tggcatagaa ttcctcggag ttgacactta ctaccaggat
     7081 acgaaggctg ggacttatct ccctgatttt ttgtagcatt ggcaaacctt ctattccagg
     7141 catgttgagg tcggtcaaca acacgtcgta ataagatttt tttaacttac tgagaagttc
     7201 aaggccatct gaagcaaaat cgatggacac atcttcatca actgtgtccc tgactaagat
     7261 ctcaagacca actctcacag caaagtgatc atcagctatt ataatcctta gcatagtatt
     7321 ataattaata gattaataag aggttaatat gaaacaaata taataaattt ttggtttgat
     7381 ccaaattctt tttgtataaa gtttgtaatt aaagtcattt gtcttgttca ggcattgatt
     7441 aaaacaggga tgagaatgtt ttatttgttg gtcaatggtt tatcggaggt gaggatttga
     7501 aatgcctata tcagggactg atattccaat ggcataaaga actgatattt actgtgtgga
     7561 attgttcctc attttcagaa taaattatgc ggaatgacat tcagtgacag tgagggagga
     7621 gcttttggtc tttctggcat agtttcagaa ttcggggctt tatggctgtt acaccatcag
     7681 gtatttgatt ttgggtgaat ttgaaattat gttcaatttt acattccaat tcaaaagtta
     7741 tttcaaaact ttacctttgt aggatgccat acccacatca ggttgtaatt gttattcctg
     7801 tatacaaacc atttcccgat cccgaagaaa tccgggcatt gagaagggca gtcagcgtat
     7861 tcagcaggca tcctatccgg ttattaggcc cagatgatat agattacagt gtgtatctgg
     7921 atgccggaat tgatagatgc tctatcattc agatgcccgg ccaccatttt cgggggatta
     7981 aagcctacaa ccgccttttg tgcagcaagg gattctatgc catcttcgac gtctttgagt
     8041 atatattaat atttcaaacg gatgcctggg ttttccggga tgaactctcc gaatggtgcg
     8101 agagaggcta cgactatatt ggatctccgt ggttgtggcg gcccgagcgg gttaagaata
     8161 gtcaaatatt ggatctgtgg ccattgatga agggacgtgt gggtaatggg ggtgtaagcc
     8221 ttcgtaagat taaggtaatg aagaaatatg ctttattggc tcgtattttt tttgccctaa
     8281 ctgccaaaaa tgaagatttt atctggttgc tggtttccct tttgcctggt gtaaggataa
     8341 aaaaaccgaa tgctgacgag gctttaaaat tctgcattga gatggaacct gaggaggctt
     8401 tgaacaggac aggcagcctg ccttttaccg tacatgccta tatgcgttat ggagctgatt
     8461 actggaaggc ttatatgccg gattaagaaa tgcttttcac atttgaatac agctccaata
     8521 tttgtcgatg taaagaaaag tggtatgccg gcaaagtaga ttagagtatc taagcttttg
     8581 atagctatag agttcaaaag ttgggaaaat gagtaatgag ggggagactt tgcattggtg
     8641 aaatgtctat gattatgttt ctatttcccg atttgcacag attattgaca tccacccttt
     8701 aggccattaa acctaattgc ctataaaaaa ttgattctat tgaaaattta gattgatcaa
     8761 gacgcaatag ctggtttttt aatcaaaaaa tattttactt gtaaacaata ataaaatgat
     8821 aaatactgtt taaagagaga aaacaatttt atgtagcatt tttatctatt tgtatctatg
     8881 agccaaattt gtacaaaaca aaattccaat tccacttcaa ataaaatcaa aaatttctcc
     8941 tacttttttg tcaatgagac accggataat cccaattctc ctataacatt gaagcagctt
     9001 ttgccggatg ttcgctttga gcctaaaaaa tccacccttg aagccatttt ggcatacagt
     9061 cgcaagagat aaccactaag cagggaaaga ttgttgtacc tacggtctgg tagaaaggaa
     9121 taatcgtcat aacacaattt tacgatcata ttctgataca aaagtatcat ggatcattga
     9181 ttggatcaga tgagtagccc ttgtgtgata ggtctggtag ttaagaaact caccctggaa
     9241 aatatacgga gaataattac atcacaaagt gggtcatatc tttaaagata tccacccgca
     9301 tggcgatatc cagatgattg atgcttttaa gtttggccaa ggagaagtcc tccacacaga
     9361 aggatgccat taccgatgca tatacaatgg ctctcttcat gttctcgaat gacagatctt
     9421 cagttctcgc cagatagcct atgaatcctc ctgcaaaagt atccccggcg ccggtcggat
     9481 ctattacttc agacacagga agtgccggga aggagaacat tctaccttcg cggaatagca
     9541 tgacaccgtg ttcgcccttt ttgatgataa ggtatttagg tcccatcctg tatatctttg
     9601 cagcggctgc agccagggaa tattctccac tgagctggcg tgcctcttcg tcgttgatag
     9661 taatgacgtc taccatcctt agtacctttt gaagactgtc ccatgcaaca tccatccaga
     9721 aattcatggt atccagaacg actagtttag ggcgtcgtgt catttgttgc agaacattca
     9781 tctgtacttc aggagtcaga ttgccaagca tgagataatc ggccgtacgg aaagattgtg
     9841 gcagtttggg ttgaaaattg gccagtacat ttaggtctgt aatcaaagtg tcccgttggt
     9901 tcatgttgtc atggtatctg cccgcccaga aaaaggattt ttcatcctta cgaatctcaa
     9961 tgcctgagat atcaatgccg agagaagtca tttcgtctaa ttcctgctgt ggaaaatcgc
    10021 cgccgataac tgatacgata ccttgatttt caacaaagaa tgaagctgat cggctgatgt
    10081 aggtggccgc tccacctatg actttacctg ctttccctct gggagtttct atatcgtcaa
    10141 atgcgactgt tccaacggta actaatttca tgaaaaaata attttgcaca aagattagaa
    10201 aaattaaata attcatttta gttttgccct gctttttgag agaagaggat aagaaagtta
    10261 acatctttcg gtattttcga caaaaagccg atcttgaaag ttagatcaaa gaagaaacat
    10321 tcctccttag ctcagccggt tagagtcatg gaattccatg attaatcagc gggtcggagg
    10381 ttcaagacct ggaagcaggc ttgaaaaaga aaagaaacat tcctccttag ctcagccggt
    10441 tagagtcatg gaattccatg attaatcagc gggtcggagg ttcaagtcct ggaaacaggc
    10501 ttgaaaaaga aagaaacatt cctccttagc tcagccggtt agagcatctg actgttaatc
    10561 agagggtcgt aggttcaagt cctacagggg gagcgagagg ggtcgagtaa tcggcccctt
    10621 tttttatata ggaaatcacc taaagatgca ttacttgtat atattatatt cagttagttc
    10681 agatatctat tatgtcggga taacgaat
//
LOCUS       DAOQQN010000057        10696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2235_length_10696_cov_5.674937, whole genome shotgun sequence.
ACCESSION   DAOQQN010000057 DAOQQN010000000
VERSION     DAOQQN010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10696
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2235_length_10696_cov_5.674937"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PLG24_03470"
     CDS             <1..456
                     /locus_tag="PLG24_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HMZ23975.1"
                     /translation="LYRIEGSRFISDDGLQAEVFGPSTLVVVCRGQKELMEAIRHCHG
                     QLTGTVMGISEELEQFRDCIAALQEKVGRLIFNGVPTGVEVCHAMIHGGPFPASGNSQ
                     YTSVGADAIKRFVRPVCFQDCPEALLPNELKDANPKGIFRKVDGVWGRH"
     gene            complement(499..792)
                     /locus_tag="PLG24_03475"
     CDS             complement(499..792)
                     /locus_tag="PLG24_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23976.1"
                     /translation="MDVISGNYDLTKAEAGPVMQAAFWDKQSDDDLPDSGPEFLYTLL
                     YRPVFDNSHNHSEITELSFGLYQYSQKLGMYIIKESRWHSDLSKGQIVLFVRI"
     gene            987..2027
                     /locus_tag="PLG24_03480"
     CDS             987..2027
                     /locus_tag="PLG24_03480"
                     /inference="COORDINATES: protein motif:HMM:NF016159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HMZ23977.1"
                     /translation="MIHLDGSFISSLMIHRVGNKSLDEGFKLTDKPIRTDPELMAVLR
                     PYFTSMLSGDELFSFTNEVNLDQNEVYHLARKIFAEPSSLAEHSKAITAHLYNQSTHP
                     RIKSGELYVAYFEEVVIDDEVTSAIGIFKSESRETFLQVDFSSELAGISHFEGIPARR
                     PDKACMIFNVASNEGFRVAIFDHLNRTSEAGFWRDDFLNLKPVGTDFYQTNYMLDVTK
                     QFVTKRYVEEHEPSKVDQIELLNRSVDYFKNNETFDRGEFCTAVFDDKDVRTSFLKFE
                     EQQQSEGKMRADETFSISLPAVKKQSRSIRSIIKLDRNFHIYVHGDKDLIQQGVDENG
                     RKYYKIYYETES"
     gene            complement(2102..2893)
                     /locus_tag="PLG24_03485"
     CDS             complement(2102..2893)
                     /locus_tag="PLG24_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HMZ23978.1"
                     /translation="MKNLFLTFLILLGCAIVLSGQKNFKAEEFTCRDGSILMYQKMEP
                     RKIKKSKKYPLVVFLHGAGERGADNKSQLVHGAKLFDDKKNRRKYPAFVLFPQCPSNE
                     YWAKVDIDRSDKEDPFRFDFRDKPNKPLESVFELIRSYLRLPSVDPDRVYIMGLSMGG
                     MGTFEAISRHPEWFAAAIPICGGGDLEFVESFAQKVPVWVFHGAKDDVVLPKYSRDIV
                     QEIIFKGGFPRYSEYSEANHNAWDKAFAEKDLLPWLFHQKKTLTN"
     gene            3223..4221
                     /locus_tag="PLG24_03490"
     CDS             3223..4221
                     /locus_tag="PLG24_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ23979.1"
                     /translation="MFSKVIVQGRLDFGNANTYDKALKLYTQRKDVYYKNEVIFNKME
                     ECFDEENLIFFVKRFNNLVSDKYWRNTVHLLKNLAEYSVTGRVEMWMMVDGKVIRHEQ
                     IRPSSDKSIVASFYKANEIVNENGDLSKAMKILDGIISKYEGHSMAHALAGRIAARQG
                     NIAFAIKKFDLATELDPCLTEAYLWKGKMLIREERYKEALPVLEKVTECSIAHQNIHW
                     IGRRLKGKCYFHLGDFEKAIFEWKLFTKRPFEKGNSNHYWKRMVTFSLGKAYLHTNQH
                     AEAILSFNNALELDPLFESVSEEEIMKYKAIAGSGQEESGNSKKSTRKSTGVKATA"
     gene            4445..5656
                     /locus_tag="PLG24_03495"
     CDS             4445..5656
                     /locus_tag="PLG24_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ23980.1"
                     /translation="MDHKPTTHHKSNKSDLFQSHDYYAIDELLTEDHILAREAVRAWV
                     KQEVSPIIEDYANRAECPTHLFKGLGEIGAFGPSIPAEYGGGGMDEIAYGIIMAELER
                     GDSGIRSMASVQGSLVMYPIFRFASEEQKRKYLPKLATGELIGCFGLTEPDHGSNPGG
                     METNIVDDGDAFILNGSKMWITNSPVADIAVVWAKDEEGVVRGMVVEKDTPGFSAPEI
                     HGKWSLRASITGELVFSDVRVPKANVFPDIRGLKGPLSCLSKARYGIAWGVLGAALDC
                     YDSALRYSRERIQFGKPLGQFQLTQKKLAEMITEITKAQLLAWRLGTLANQGKATPAQ
                     ISMAKRNNVHMALEVAREARQIHGGMGITNEYPIMRHMMNLETVLTYEGTHDIHLLIT
                     GMDVTGFNAFS"
     gene            5889..6878
                     /locus_tag="PLG24_03500"
     CDS             5889..6878
                     /locus_tag="PLG24_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ23981.1"
                     /translation="MYLTINLYSQEVIRLTNPSFEDIPGHSRPPIGWRDCGAFLFPGE
                     SPPDVQPSGSWEVRKPANNGDTYLGMVVRENDTWESVSQQLSGTLKKGKTYTFDLFLC
                     TSEVYMSFTKANRSTESNYTTPAKLRIWGGNSLCSKEVLLAESSLITNNNWKKYYFTF
                     KPTKNVNYIVLEAFYKTPTLVPYNGNILVDNASNIVEVIKESPVTVKELTQKVTPGQI
                     IKLDQLYFKADNSSLDKNSHKVLNEVVDFMQRNEAVKIEIGGHTNGVPSVEYCQKLSS
                     ARAREVANYIINKGIDKNRVTYKGYGKSKPIASDRTPEGRKKNQRVEIKILST"
     gene            complement(6903..8573)
                     /gene="asnB"
                     /locus_tag="PLG24_03505"
     CDS             complement(6903..8573)
                     /gene="asnB"
                     /locus_tag="PLG24_03505"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF006949.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HMZ23982.1"
                     /translation="MCGILGIFDLRQSPDSLRAQALAMSRKQRHRGPDWSGIFTCNNA
                     ILAHERLSIVDPTSGKQPLFSQDGAIVLAVNGEIYNHRQLKKGLTTPYPFQTQSDCEI
                     ILALYQEKGIDFIEDLNGIFAFALYDHANDSYLIARDHMGIIPLYMGWDEYGNFYVAS
                     EMKALSGICNKIREFLPGHYLYSRDGEMKKWYKRDWMEYDNVKDNETDIQKLRSAFEE
                     AVHRQLMSDVPYGVLLSGGLDSSIVSAIAKKYAARRIETEDLKDAWWPQLHSFAVGLK
                     DSPDLAASRKVAEHIGTIHHEIHFTIQEGLDAISDVIYHIETYDVTTIRASTPMYLLA
                     RVIKSMGIKMVLSGEGSDEIFGGYLYFHKAPDARAFHEETVRKVGKLHLYDCLRANKS
                     LAAWGVEGRVPFLDKEFMDVAMRINPKDKMAGNGKIEKYILRKAFEDYLPHEVAWRQK
                     EQFSDGVGYSWIDTLKLITAERVTDEQLNNAAHRFPLNTPTTKEEYYYRSLFAEHFPG
                     SEEAACVPFEASVACSTAIALEWDEAFKKMKDPSGRAVSSVHEAGYKE"
     gene            9122..9586
                     /locus_tag="PLG24_03510"
     CDS             9122..9586
                     /locus_tag="PLG24_03510"
                     /inference="COORDINATES: protein motif:HMM:NF021538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HMZ23983.1"
                     /translation="MTFYQILILVHVLSAILGLGPGFIMIYVVTRASTMTELRHAYLI
                     RNRLHIFVMIGGTILLLTGLAMGILNPYWFRQGWYITSLTLFLIALGFGPAVLSPLSK
                     PIKKLLKEHQGDDIPVHYNELIRKLFFYERIENVLFIIIIVLMVLKPDFQLW"
     gene            9801..10406
                     /locus_tag="PLG24_03515"
     CDS             9801..10406
                     /locus_tag="PLG24_03515"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMZ23984.1"
                     /translation="MDREKNTKNGLDVAFWNQRWLDGTTGWDIGYASPPITDFLSGYD
                     QRDARILIPGCGNAYEAEFLSDNGFSDIVLLDIAPEAVRRLKEKFTLSKAVTVVCGDF
                     FEHEGKYDLILEQTFFCAQVLERREEYVEKVHTLLNPGGILAGVLFNIDFEQAGPPFG
                     GDIEEYRRLFEPYFNIAIMQPCYNSIAPRAGSELFVKMIKK"
     gene            10433..>10696
                     /locus_tag="PLG24_03520"
     CDS             10433..>10696
                     /locus_tag="PLG24_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23985.1"
                     /translation="MSLKQALIDEIEREAVSTGKMLERIPEDKFDWSPHARSMAMKAL
                     AVHIVGIAAYTDVIATTDYLDIGERRPPQPEINTTQDIVDAFES"
BASE COUNT         3107 a         2194 c         2368 g         3027 t
ORIGIN      
        1 ctttacagga tcgaaggaag tcgttttata agtgatgatg gcttgcaggc agaagttttc
       61 ggaccatcga ccctcgtagt tgtctgtcgc ggtcagaaag aattgatgga ggctatccgt
      121 cactgccatg gacaattaac cggaacggta atgggaatat ctgaggagtt ggaacagttt
      181 agggattgta tcgccgcttt gcaggaaaaa gtcggccggc tgatattcaa cggcgtgcct
      241 accggggtag aagtgtgtca tgccatgatc cacggcggtc cgtttccagc atccggcaat
      301 agccaataca cctcagtcgg tgctgatgca ataaaacgtt tcgtccggcc ggtgtgcttt
      361 caggattgtc cggaggcatt acttcccaat gaattgaagg atgcaaatcc caagggcatc
      421 ttcagaaaag tcgatggagt gtggggaaga cattgattgg gcattatacg gtaaatacag
      481 gttgacctta ccggaatatc agattcggac aaacaacaca atttgccctt tggataaatc
      541 agaatgccac cgggattcct taataatata catacccaac ttttgggagt attgataaag
      601 gccaaagctt aattctgtga tttcagaatg gttatgacta ttgtcaaata caggccgata
      661 taacaaggta tatagaaatt ctggtcctga atcagggaga tcatcatcgg attgcttatc
      721 ccaaaaagct gcttgcatca ccggacctgc ctcagccttt gtaaggtcat aattaccgga
      781 aattacatcc actaagcgca attaatcagc cggtatcttt tccggcaatt atatcatata
      841 ggcaattgtg ggcgttatct ttgttataac cgcaaagttt tcaaattgca tatttatatt
      901 ttcaggaaaa attatcaaag cccttttatt tttggattgt cttcctaagc gggaagtccg
      961 atataaatta taaatttgat tccggtatga tacatttaga cggcagtttt atttcatccc
     1021 tgatgatcca ccgagtgggt aataaatctc ttgatgaggg atttaaactt acggataagc
     1081 ctatccggac tgatccggaa ttgatggcag tcctccgacc ctattttacc tctatgctgt
     1141 ccggtgacga actatttagc tttaccaatg aagtcaatct tgatcagaat gaagtatatc
     1201 atctggcaag aaagatattt gccgaaccat cgtcacttgc tgaacacagc aaggcaatta
     1261 cagcccatct gtacaatcag agcacacatc cacgtatcaa gtccggtgag ctttatgttg
     1321 cttattttga agaagtagtc attgacgatg aagtgacttc agctatcggc atctttaagt
     1381 ccgaatcgag agaaactttc ctgcaagtgg atttctcatc agaacttgcc ggtatctctc
     1441 actttgaagg aatcccggca cgacggccgg acaaggcttg tatgatcttc aatgtggcat
     1501 caaatgaagg tttcagagtg gcgattttcg atcacctgaa caggacgtca gaggcaggat
     1561 tctggaggga tgattttctc aatctaaagc ctgtcggcac cgatttttat caaaccaatt
     1621 atatgctcga tgtcaccaaa caatttgtca ctaaacggta tgttgaagaa catgaaccct
     1681 ccaaggtcga tcagatagaa ttgctcaatc gttctgttga ttattttaag aataacgaaa
     1741 cattcgatcg gggagaattc tgtactgccg tttttgatga caaggatgta cgtacttcat
     1801 tcctcaaatt cgaagagcag caacaaagcg aaggaaagat gagggcagac gagaccttca
     1861 gcatttcctt gcctgcagta aaaaagcaaa gccgaagtat tcgcagcatc atcaaattgg
     1921 acaggaattt tcatatctat gtacatggtg ataaagacct catccagcaa ggagtcgatg
     1981 aaaatggtcg taaatactat aaaatctatt acgaaacaga gtcgtgatta tcacggaaag
     2041 aaattggtca ctttgcctgg cagtgcaaga ttcatacaca tgcagttgta aaaaaaactt
     2101 ttcaattcgt cagggtcttt ttttgatgga acaaccacgg caggagatcc ttttccgcaa
     2161 acgccttgtc ccaggcatta tgattggctt cagaatattc agagtaccgt ggaaaaccac
     2221 ccttgaaaat aatttcctgt acaatatctc ggctatattt gggcaataca acatcgtcct
     2281 ttgctccatg aaatacccac acgggaacct tttgggcaaa tgactctaca aattcaaggt
     2341 ctccgccacc acagatcgga attgcagcag caaaccactc cggatgcctg cttattgctt
     2401 caaatgtacc catcccaccc atggaaaggc ccatgatata aacccggtcc ggatcgacag
     2461 aaggaagccg gagataactc ctgatgagtt caaatacgga ttccaggggc ttgttgggct
     2521 tatccctgaa gtcaaatcgg aagggatcct ccttatcact tctatcgata tccacttttg
     2581 cccagtactc attggatggg cattgcggaa ataatacaaa ggcgggatat tttcgcctgt
     2641 tctttttgtc atcaaatagt tttgcaccat gaaccagttg actcttattg tcagcaccac
     2701 gttcaccggc accatgtaga aatacgacaa gcggatattt cttggatttc tttattttac
     2761 gaggctccat tttttgatac atcaatatac tcccgtcacg gcaggtaaat tcttctgctt
     2821 taaaattttt ttgcccggaa agaacaatgg cacatcccag gaggattaga aatgtcaaga
     2881 aaagattttt catatcagaa gccaattatt cctcaaagat cataaaaata cccaatcaga
     2941 cattcaaata tttttaaaaa cggaaagaga gattttgagt tcatatatat gttatccgca
     3001 agtcataccg ataaataaaa tataaaataa tttcatatat attaaacaat ttctacttca
     3061 gataagacat tgttatatat aaataaataa tatgtttaca aacacttgct ttgaattttt
     3121 acgaatcgta tctttgtatt tcttgtgtga atcagagcgg aggtgttgtt gcgtgattgc
     3181 tgctgcattt ctgatagcac attctaacca tagtattgtt ttatgttctc aaaagttatt
     3241 gttcagggtc ggttggactt tggaaacgcg aatacctatg acaaagctct gaagttgtac
     3301 acacaacgga aggatgtcta ctataagaat gaagtcattt tcaacaagat ggaggaatgc
     3361 tttgacgagg aaaacctgat attctttgtc aaacgtttca acaatcttgt ctccgataaa
     3421 tattggcgaa atacagtaca tcttttgaag aatctggcgg aatattctgt tacgggtagg
     3481 gtggagatgt ggatgatggt cgatggaaag gtaatccgcc atgaacagat caggccttcc
     3541 agcgataaaa gcatcgttgc ttctttttat aaggccaatg aaattgtcaa tgaaaacggt
     3601 gacctttcca aagctatgaa gatattggat ggcatcatca gcaaatacga aggacattcc
     3661 atggctcatg cgcttgcagg gaggattgcg gcacgacagg gaaatattgc ctttgctata
     3721 aagaagtttg atctagccac agagctcgat ccctgcctta cggaagcata tctttggaaa
     3781 gggaaaatgc ttatccgtga agaaagatac aaggaggccc ttcctgtttt ggaaaaggtt
     3841 acggagtgtt ctattgcaca ccagaatatt cactggatag gtcgcaggtt gaaaggcaaa
     3901 tgttattttc accttggaga ttttgaaaag gccatctttg aatggaaatt gtttaccaag
     3961 cgaccttttg aaaaaggcaa ttcgaatcat tactggaagc gaatggtgac attcagcctt
     4021 ggaaaggcat atttgcacac caatcagcat gcggaggcaa ttctttcttt taataatgct
     4081 cttgagttgg atcccctctt tgaatccgtt tcagaagaag aaattatgaa atacaaagcc
     4141 atcgcaggca gtggccagga agaaagcggc aatagtaaga aatccacccg aaagtctacc
     4201 ggggtcaagg cgactgcctg aaagagggat tttgaagctt aatatattca ttattctatc
     4261 ttgtggggga ctatagaaaa gttcttttgt atttgcaatc aatggtattg tataatcaat
     4321 ctcaccttga agggagtccc tgctttttcc ggatggagct catcccgatt ttataatctt
     4381 tttctttacg aatacaggct acctactaaa ttttagtatt tttgcagcca aaatcaaaaa
     4441 agacatggat cataagccga caactcacca caaatcaaac aaatctgacc ttttccaatc
     4501 ccatgactac tatgctatcg atgaattgtt gaccgaagac catattctgg cccgggaagc
     4561 tgtcagggca tgggtgaagc aggaagtaag cccaatcatc gaagattatg ccaaccgtgc
     4621 agaatgcccg actcatcttt ttaaggggct gggtgaaatt ggcgctttcg gccccagtat
     4681 tccggcagaa tacggaggcg gaggcatgga cgaaattgcc tatggtatca ttatggccga
     4741 actggagcga ggagattcag gaatcagatc gatggcctca gtgcagggta gccttgtgat
     4801 gtatcccata tttcgatttg caagcgaaga acagaagaga aaatatcttc ccaaactggc
     4861 aaccggtgag ctgattggtt gttttgggct tactgagccg gatcatggtt ccaaccccgg
     4921 aggcatggag accaacatcg tggatgacgg agatgccttc atcctcaatg gttcaaaaat
     4981 gtggataacc aattcgcctg tagcagatat cgctgttgtg tgggccaagg atgaggaagg
     5041 cgtcgtgagg ggcatggtgg tcgaaaagga tacacccgga tttagtgcac ctgagatcca
     5101 tggtaaatgg tcactgagag catctatcac gggggaactt gtgttttcgg atgtgagagt
     5161 gcctaaggca aatgtcttcc cggatatcag aggattgaaa ggcccgcttt catgcctttc
     5221 aaaggcccgt tacggtatag catggggtgt attgggcgct gcactggatt gttatgacag
     5281 tgctttgaga tattccaggg aaagaattca atttgggaag cccctgggcc aattccagct
     5341 tactcaaaag aaactggcag agatgatcac cgaaataact aaggcacaac tattggcatg
     5401 gcggctgggc acacttgcca atcaggggaa agcaacaccg gcacagatat ccatggcgaa
     5461 gagaaacaat gttcatatgg cactggaagt ggccagagaa gcacgccaaa tacatggcgg
     5521 catgggcatt acgaatgaat accctatcat gaggcatatg atgaatcttg agactgtcct
     5581 tacatatgaa ggcactcacg atattcacct cctgattaca ggcatggatg tcacagggtt
     5641 caacgccttt tcatgaaaat ttggtatatc aaacttagga catcaatggt gtcgcccccg
     5701 ggtcggaagg aagagtgaat atgaactgtt tttgtgattt cgctgttatt ctcttgttaa
     5761 aaatatcccc gggtaggcta aataccgttg acggacgttt ttagaaatac tgttgacaat
     5821 agaccatgat agttatcttt ttgaggttgc aaaagtattc tgggaagatt tttgggtgta
     5881 tatttttcat gtacctgaca ataaatctat acagccagga agtaattcgc cttactaatc
     5941 cttcctttga agatattccc ggtcatagtc gtccaccaat tggttggcgg gattgtggtg
     6001 cttttttatt tccgggagag agcccacccg atgttcagcc aagtggatcg tgggaagttc
     6061 gcaagccggc aaacaacggt gatacctatc ttggaatggt cgtgcgcgag aatgatacct
     6121 gggagtcggt atctcaacaa ttgtcaggta ctttgaagaa aggcaagacc tatacttttg
     6181 accttttttt gtgtacatct gaagtgtata tgagctttac caaagccaac agaagtacgg
     6241 aatccaacta cactactccg gccaaactca ggatctgggg cgggaatagc ctgtgtagta
     6301 aggaagtatt gctggctgag agctctctta ttacaaataa taactggaag aaatactatt
     6361 ttacattcaa acccactaag aatgtgaatt atatcgtctt ggaagctttc tacaagacac
     6421 ccacccttgt tccatacaat gggaatatcc tggtcgacaa tgcaagcaac atcgtggaag
     6481 tcatcaagga gtcacccgtt accgtaaagg aactgactca gaaggttact cccggacaaa
     6541 tcatcaaact ggaccagctt tatttcaagg cagataactc ttcacttgac aaaaatagtc
     6601 acaaggtgct aaatgaggta gtggacttca tgcaaaggaa tgaagcggta aaaattgaaa
     6661 ttggtggtca taccaatggt gtcccctccg tggagtactg tcagaaattg tcctcagcca
     6721 gagccaggga agtagctaac tatattatta acaagggcat cgataaaaac agagtcacct
     6781 acaaaggtta tggtaaaagc aaacctattg catccgacag aacaccggaa ggacgaaaga
     6841 aaaatcaaag agtagaaatt aaaatcctca gcacctgatc gcaggaattt cctcgcgata
     6901 tgtcattctt tgtagccggc ttcatgtact gaagacactg cccgcccact tggatccttc
     6961 atctttttaa atgcttcatc ccattcgagc gcaatagcgg tactgcaagc cactgacgct
     7021 tcaaaaggca cacaggcagc ctcttcactt cccgggaagt gttcggcaaa taaggaacgg
     7081 taataatact cttccttggt cgtcggagta ttcagaggaa atctatgggc agcattattc
     7141 aattgttcgt cggtgaccct ctcagccgta atcaacttga gcgtgtctat ccaactgtaa
     7201 ccgactccat ccgaaaattg ttccttctgc ctccatgcca cttcgtgagg aaggtagtcc
     7261 tcaaaagctt ttctgagaat gtatttttcg atctttccgt taccggccat tttgtccttt
     7321 gggttgatcc tcatggctac atccataaac tccttgtcca ggaatggcac cctgccttct
     7381 acaccccagg cagccaacga cttgttagcc ctcaggcaat catagaggtg caacttgcca
     7441 acttttctta cggtttcttc atggaaagcc ctggcatcag gtgccttatg gaagtataaa
     7501 taaccgccga agatttcgtc cgatccttct cccgaaagaa ccattttgat acccatcgac
     7561 ttgatgaccc gggcaagaag gtacatcgga gtgctggcgc gaatcgtagt aacatcataa
     7621 gtctcaatat gatagatgac atcggagatt gcatccagcc cttcctgaat ggtaaagtga
     7681 atctcatggt gaatagtgcc gatgtgctca gctacctttc ttgatgcagc caggtcagga
     7741 gaatctttca agccaactgc aaagctgtgc aattgcggcc accatgcgtc cttcagatcc
     7801 tccgtctcaa tccttcgtgc agcgtacttt ttggctatgg cacttacaat actggagtct
     7861 aacccacctg aaaggagtac accgtagggc acatcggaca ttaattggcg atggacagct
     7921 tcttcaaaag cgcttctcag cttctggata tccgtttcat tgtccttgac attatcgtat
     7981 tccatccagt cgcgcttata ccatttcttc atctccccat ccctggaata cagatagtgc
     8041 cccggcaaaa actcccggat cttgttgcat ataccactca gtgccttcat ctcggacgcc
     8101 acataaaaat tgccgtattc gtcccatccc atgtaaagag gaatgatgcc catgtggtct
     8161 cgggcgatca gatatgaatc attggcgtgg tcatacaagg caaaggcaaa gatgccattc
     8221 aggtcttcta tgaagtcgat acctttctcc tgatacagag caaggattat ttcacagtca
     8281 gattgtgtct gaaagggata tggcgttgtg agcccttttt tcaactgacg gtgattgtat
     8341 atttcaccat tcacggccag cacaattgct ccgtcctggc taaacaacgg ttgcttgccg
     8401 gaggtagggt ctacaataga aagcctttcg tgtgccagga tggcattgtt gcaggtaaat
     8461 atgccggacc agtcaggtcc acgatgcctt tgttttctgg acatggcaag cgcctgagca
     8521 cggagggaat caggactttg cctgagatcg aagattccta ggataccaca cattggatta
     8581 taattttaaa gggcaaaggt agttatttct aacaaatccg ggttcatttg cacggtaaag
     8641 tgtatttcac aatttattgg ataaaaatca ggatcaggct gaagataagt cttgcaacgg
     8701 accggccttg tccggagttt actaataatt ggggttatct catcttggca cattaactta
     8761 tatcatttgt tgtcaaggga attggatgac ctgatgaata gcctcaaatt aacgcggtat
     8821 actaattttg tagggctact tttttttaat attcctgatt attatgaaat atttgtaata
     8881 tataaaaaca atataatcaa attaatataa taattttgtt ttaatattca cacgctttat
     8941 aacggcccgg tatatttgtt gattttaaga aaaacggaat cggtcattac cccatttgat
     9001 gtagtctctc caaaaagtat gtttattgca ctatattagc agggaaaaat tagtcggtta
     9061 ttatttcctg gtttgaatta tacattaatt catgctatgt atttatgaaa tttcagcaaa
     9121 tatgactttt tatcaaatcc tgattctggt acacgttctt tcggccattc tcggcctcgg
     9181 gccgggattc atcatgatat atgtggtcac aagagcgagt actatgacag aactaaggca
     9241 tgcttacctc attcgaaacc gactacatat ttttgttatg attggtggta cgattctttt
     9301 actgaccggg ttagcaatgg ggattctcaa tccttattgg tttcgccagg ggtggtacat
     9361 caccagccta accctgtttc tgatagcttt gggatttgga ccggcagtgt tatcgcctct
     9421 ttccaaaccc ataaagaaac tgttgaagga gcatcagggt gatgatattc cagtccacta
     9481 taatgagctg atcagaaagc tgttctttta tgagcggatt gaaaatgttc tatttattat
     9541 cattatcgta ttaatggtgc tgaagccgga ctttcaactt tggtgagcat gcaagttgtc
     9601 tttcttaatc ttttcgtagc ttgtgatgtt ataccaggat gatttatata atagtccata
     9661 aatttttggt ataataacga atgtaccttc ggtattaatt atattttgtt ctaaagggga
     9721 aaataattta atatttccct ttgaaattaa catgcagcat ttgaaaggat gcctaattat
     9781 gaccagttaa atctttttta atggatagag aaaaaaacac taagaatggt ctggacgttg
     9841 cgttctggaa tcaacgatgg cttgacggaa ccaccggctg ggatatcggt tatgcatctc
     9901 cgcctattac cgacttttta tcagggtatg accaaagaga tgcccgaatc ttaattcctg
     9961 gctgtggtaa tgcctatgaa gcagaatttc tttctgacaa tggattttct gatatcgtat
    10021 tactggatat tgcgccggag gccgtcaggc gcttgaagga gaaatttacc ttatcgaagg
    10081 ctgtgactgt ggtgtgtggt gactttttcg aacatgaggg taagtacgac ctgatccttg
    10141 agcaaacttt cttttgtgca caggtacttg aaagaaggga ggaatatgtg gagaaagttc
    10201 atacattgct caatcccggc ggtatcctgg caggagttct ctttaatatc gattttgagc
    10261 aagcgggacc gccatttgga ggtgatatag aggaatacag aagactgttc gaaccgtatt
    10321 tcaatattgc gatcatgcag ccatgctaca atagtatagc gcccagagcc ggttctgaat
    10381 tgttcgtcaa aatgataaag aaataattta tttaacaaca aaaaccattt agatgtcatt
    10441 aaaacaagca cttattgacg aaattgaacg agaggcagta agtaccggga aaatgcttga
    10501 gcgaattccg gaagataaat ttgattggtc gccacatgcc aggtcaatgg ctatgaaagc
    10561 tcttgccgtg catattgtgg gaatagcagc atatacagat gtaattgcaa ctaccgatta
    10621 tctggatatt ggggaaagaa ggccgccaca accagaaatt aacacgactc aggatattgt
    10681 cgacgcgttt gaatca
//
LOCUS       DAOQQN010000058        10559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2279_length_10559_cov_5.811691, whole genome shotgun sequence.
ACCESSION   DAOQQN010000058 DAOQQN010000000
VERSION     DAOQQN010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10559
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2279_length_10559_cov_5.811691"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..461
                     /locus_tag="PLG24_03525"
     CDS             222..461
                     /locus_tag="PLG24_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HMZ23986.1"
                     /translation="MLTKIIKIGNSKGLRLSKTILEKYNIGETVNMRMEENYIIIEPN
                     TKPRTDWVKSFKEMRKNGDDELLIQDIFEEESKWV"
     gene            458..781
                     /locus_tag="PLG24_03530"
     CDS             458..781
                     /locus_tag="PLG24_03530"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HMZ23987.1"
                     /translation="MKFSQYEIVLVNLEPTLGSEISKTIPCVVVSPDEMNHYLNKIVI
                     IPITSTLKKYPTRISISNHNVTGMAAVDQIRTIDKRRIVKIIGSVEIETIEKIKKTID
                     ETYVK"
     gene            complement(1054..2298)
                     /locus_tag="PLG24_03535"
     CDS             complement(1054..2298)
                     /locus_tag="PLG24_03535"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HMZ23988.1"
                     /translation="MKATIIAIGDELLIGQVLDTNSQWIARQCTELNIEIVRKTTVSD
                     GIEPIVAAIQEGVALSDIIIMTGGLGPTSDDYTPRAIAEYFGMEIKFHPPTWERILAY
                     FLSRGREPNEALKAQALLPERITFLVNDMGTAPGMYHHWEDKHLVSFPGVPYEMKHLF
                     TDRFVPIIKPYLGDNHVKSRTILTVGKGETDIATAISDIESNLDSRLSIAYLPDIGRV
                     RVRITGRDTNGEDLNGLINRTGDAIIDRLKDSVYGEGKLTLSEAIRDIMVEKGLTLGL
                     AESCTGGNISRQVVSASGSSAYYKGGIVSYSNQAKQSLLGVSPDSLEQYGAVSEQVAK
                     EMAEGALNVFHSDIAASVTGIAGPDGGSESKPVGTFWVGLAMKDKETRAFKINFNRDR
                     ERNIEFVSIFVMNTIRMSILSM"
     gene            complement(2295..3191)
                     /locus_tag="PLG24_03540"
     CDS             complement(2295..3191)
                     /locus_tag="PLG24_03540"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HMZ23989.1"
                     /translation="MRNIHFIVNPISGKGRQTKELNEDFLKSMFPSDQFSITMSIIPH
                     KGATGILTEQAIAAGAETIVACGGDGTINEVARHLVHTDIRLGIIPRGSGNGLASHLA
                     IPKNLLSCLNIIRTGNTTKIDTALAGNFRFFSNCGFGFPTELIRQYELIPQRRLSGYI
                     KAGLSSIHAIKDSAMLKITCNGKIMNARHLFVSNSNKMGYGMTLAPGASLKDGLFNVQ
                     VIGCETLISFGIYGLMVLMGKGDKKKEVTDMLATGVEVEGFKPLQAQIDGEFHQLNEQ
                     KIQIINEPASLNVLIANTSNIP"
     gene            complement(3484..4788)
                     /locus_tag="PLG24_03545"
     CDS             complement(3484..4788)
                     /locus_tag="PLG24_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ23990.1"
                     /translation="MNKTLIVAKREFATRALKKKFLLITLFMPLIIAIFSGSVGFIMS
                     YKGNSGTPILLVDPNKIIPESSDSWDNLTFTRIDYNEEVIKKELEGNFKAAIVLPEIS
                     SNLSEQYSPAMWTVKRLDLQTKASIEKFIRVSIKNKKLELLGLDKNKLDDIDQPIHLL
                     EENISGKKESGSEVAAVIGGIFGFFLYLLLTINGSMIMRSVMEEKTNRIIEVMISTVT
                     PKQLMFGKIIGVGLVGLFQILIWCITTPLFIFLITFLFAGDMDMSGMMATGATGEVVQ
                     TQVANSFMGDIIGQLQAVQWWVLIPTIFLFFLLGYLMYSSMFAAIGSTMGDDQGEGQS
                     LTFLVIMPLLFGFYISMAAISSPNSSMAVWSSIIPLFSPIVMPVRLAFDPPVWQIVTS
                     LISLALSSWFFAWLSARIYRVGILMYGKKATFKDMVRWFKAK"
     gene            4933..5586
                     /locus_tag="PLG24_03550"
     CDS             4933..5586
                     /locus_tag="PLG24_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ23991.1"
                     /translation="MEFNINALRTFVDQKKNLVIGILAGLAILIAGYFIYKAFFVAPK
                     NKVAADQIYQAQIMFDKDSFQMALNNPGVGGTGFLKIIENYGGTETGNLARYYAGVCY
                     LRLGDYDKAIKYMNEYKPAGEITPAMTYEVLADAYSEKKDDNKALSYYEKAAKSADNE
                     LTGPYTLKKLGMFQEKLGKYADAAKSYKKIMDVYGQTADANNIEAYYERAMLLANKK"
     gene            6295..6966
                     /locus_tag="PLG24_03555"
     CDS             6295..6966
                     /locus_tag="PLG24_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ23992.1"
                     /translation="MRQFYIALFLLVTVCLKGQDIKFGVEAGFNSVKSKLPKEYNSYF
                     KQKPSFIIGIPVDISFTKLFNIHSGLYFSQLGTKAHYLQDNRPYSYSESYRNGTINSI
                     IIPANYFYEFKINNIGLFGGIGFEGEFNISGKSVGYDIQESSIPWFDNYHKDFEKKAK
                     IGKDLPLIAFGFNVYGGVKFKDAFFVKLFLTQGLSNIDITPSYSSVPEKQQQFGLTIG
                     YMFQK"
     gene            complement(7335..8372)
                     /locus_tag="PLG24_03560"
     CDS             complement(7335..8372)
                     /locus_tag="PLG24_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23993.1"
                     /translation="MTQQITPFTGADGKPKSFIQRPEGKAGLLVGAGILAGAGYLLYK
                     ALPYLIELAKNTLYLAGMVIVLGALLYMVFDPKMRNLIWYMYKSVMRKITGWFVQIDP
                     IGILKSYIEDLENNLKKMGTQIGNLKGQMRKLQNIMDENKASIDINLKMASKAKEMDK
                     DQHMILSSRKAARLTDTNEKYQVLHQKMEVLYRILTKMYSNSEILLEDTRDQVKVKEQ
                     ERAAIRASHSAMKSAMNIIAGNSDKRAMFDQAMESIADDVAMKVGEMERFMEMSSNFM
                     DSIDLQNAVFEEDGMKMLEQWEKESTLMLAGGRKSESTTLDLNAPRAERAYVEREKKN
                     NDYDQFFEEEK"
     gene            complement(8377..8937)
                     /locus_tag="PLG24_03565"
     CDS             complement(8377..8937)
                     /locus_tag="PLG24_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ23994.1"
                     /translation="MSGKLNQLSNLFKLSPEADQKSLEFMMMALEKNNQEGFDYIEFK
                     QAFNALLQLPLDEVMSVKSAFTTGNTLGLTKESLLRSADFYKQVIHKEKVQFDAALKN
                     QREQKIDGRKAEQQKLQDQISRNKDLINKLQAEIDQYQKSIDGVDEEIEAAIAKIEDS
                     RYKFEHTFSEIITQLSKDIELINQNI"
     gene            9205..>10559
                     /locus_tag="PLG24_03570"
     CDS             9205..>10559
                     /locus_tag="PLG24_03570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HMZ23995.1"
                     /translation="MSTNAASMSHPKGLYLLFFTEMWERFSYYGMRAIFVLYMVNGLK
                     MAENDASNIYGSYTGLVYLTPLLGGYLSDRFLGNRRSIEIGGILMAIGQFLMFFSAST
                     SGATAITLMWAGLTALIIGNGFFKPNISTMVGQLYPEGDRRVDSAFTIFYMGINLGAF
                     FSPLVCGTLGEKIAFKWGFFAAGIGMLIGVLLFLAQKNKYLVTDKGEPIGMPSKKLDT
                     KNIAYLVGSILLIFFLLNFKSMFNVDTDIIGYFIFGSMIIVPAIIISDKSLTSTEKSR
                     IWVIFILAFFVIFFWGAFEQAGASLTIFADKQTDRHIFGWEMPASYFQSVNPLSIIVL
                     APLFSAMWIKLGNRNMEPATPRKMALGLSLIALGYVLIAFAVKGVDASTKINMFRLFS
                     LYVVHTMGELCLSPIGLSMVAKLSPARFSSLLMGTWFLANAAANKFAGTLSELIPSES
                     NP"
BASE COUNT         3070 a         2033 c         2101 g         3355 t
ORIGIN      
        1 tttaattatt tcaggatttt acagaaactt actttttaca tgtattctgt cattttactt
       61 ccaatcagaa aaatcatgtt cacttttcac cgaattataa tgattatcag atgtttataa
      121 atcaattggc gagtcacgaa cgcatgaaac atatgttctc accaatataa taaatctgta
      181 aatgtgatta caatgtaata actttgcaat aaaaatttac catgctaacc aaaataatta
      241 aaataggcaa ttcaaaggga cttcgattaa gtaaaactat tctagagaag tataatattg
      301 gagaaactgt aaatatgagg atggaagaaa actacattat aatagaaccc aatactaaac
      361 cacgaaccga ttgggtaaaa tctttcaaag aaatgcgaaa aaatggagat gatgaattat
      421 taatccaaga cattttcgaa gaagaatcaa aatgggtatg aaatttagtc aatatgaaat
      481 tgtcctcgtt aatttagagc caactttggg aagtgaaata agtaagacaa taccttgcgt
      541 ggtagtatca cccgatgaaa tgaaccacta tttgaacaaa attgtaatta ttccaataac
      601 ctcaacgctt aagaaatatc caacaaggat tagtatatca aatcataatg taacgggaat
      661 ggcggccgtg gatcaaataa gaactattga taaacggaga attgttaaaa tcataggaag
      721 cgtagaaatt gaaacaatag aaaaaataaa aaaaacaatc gatgaaacat atgttaaata
      781 ataatactgg tgagaacaat gcactggacg ttattagata aagcatcttg tcttcaggaa
      841 atctaccaat tttcaaaaag accggtaatt ctttttagca attatttcgc aggtgaaagg
      901 aaaaggactc cctccggtgt ttaacaggct ttcatgacga tgatattttg ttctgccagg
      961 ccctttcgtc aaagttattc aggcaactca catgtccggg tatttaccgg agtaaggata
     1021 tatcctggca tttttaggat aggatattgt atcctacatg gaaaggatag acatacggat
     1081 tgtattcatc acaaaaatgg atacaaattc aatattcctc tctctgtccc gattgaaatt
     1141 gatcttgaat gcgcgggttt ccttatcttt cattgccagg ccgacccaaa acgtacctac
     1201 cggtttggat tcacttcctc catccgggcc tgcaattccg gttacacttg ccgcaatatc
     1261 tgaatgaaat acatttagtg caccttctgc catctccttt gcaacctgtt cacttaccgc
     1321 accatactgc tccagactgt ccgggcttac accaagcaag ctttgttttg cctgattgga
     1381 ataagataca attcctcctt tataatatgc ggaggatccg gatgcagaaa caacctgtct
     1441 gctgatattc cctccggtac agctttctgc cagtccaagt gtcaaacctt tttctaccat
     1501 tatatccctg atggcctctg atagggtaag tttaccttca ccatacacgg aatccttaag
     1561 ccgatcaatg attgcgtcgc cggttctgtt tatgagtcca ttcaagtctt ctccgttcgt
     1621 atccctacct gtaatccgga ctcgcacacg cccgatatcc ggtaaatagg caatggagag
     1681 tctggagtca agatttgatt caatatcact aattgctgta gcaatgtccg tttcaccttt
     1741 gccaacggtc agaatagtcc tggacttaac atggttgtca cctaaatatg gtttaatgat
     1801 ggggacgaat cggtcggtaa aaaggtgctt catttcgtag ggtacaccgg ggaaggagac
     1861 cagatgctta tcttcccaat ggtgatacat tccaggagcg gtacccatat cattgacgag
     1921 aaaagtgatt cgttccggca acaaagcctg ggctttcaag gcttcgttag gttcacgtcc
     1981 ccttgacaga aaataggcta gtattctttc ccaggtcggt gggtgaaatt tgatttccat
     2041 cccaaaatat tcggcaatgg ccctgggggt ataatcgtca ctcgttggac caagcccccc
     2101 tgtcatgatg ataatatctg ataaggcgac tccttcctgt atggccgcta ctattggttc
     2161 aattccgtcc gaaaccgtag tcttccttac tatttcgatg ttcagttcag tacattgacg
     2221 ggcgatccat tgactgttgg tgtccagaac ctgaccgatc aacaattcat cgccgatggc
     2281 tatgatggtt gctttcatgg tatgtttgat gtgttagcta ttaaaacatt cagggatgcc
     2341 ggttcgttga tgatttggat tttttgctca ttcaattgat gaaattctcc atcgatctgt
     2401 gcctgtaaag gtttaaatcc ctctacctca actccggttg cgagcatgtc agttacttct
     2461 tttttcttgt ctccctttcc cataagaacc attagtccat aaattccgaa tgaaataagg
     2521 gtttcacacc caataacctg tacattaaac aatccgtcct taagggatgc tcccggtgca
     2581 agtgtcatgc catatcccat cttgttactg ttggagacaa agagatgtcg ggcattcatg
     2641 atcttaccgt tgcaagttat cttcaacata gcagaatcct tgatggcgtg aatggatgat
     2701 aatccggcct tgatgtatcc cgacaaacgc ctttgaggaa ttagttcgta ctgcctgatt
     2761 aattctgtag gaaagccaaa accgcagtta ctgaagaacc gaaagttacc tgccaatgca
     2821 gtatctattt ttgttgtatt gcctgtgcgg atgatattaa ggcatgaaag cagattcttt
     2881 ggaattgcca ggtggcttgc cagcccattg ccggacccgc gtggtatgat gcctaggcgg
     2941 atatcggtgt gtacaaggtg cctggctact tcattgatcg taccatcccc gccgcatgca
     3001 actatggttt cagcaccggc agcaatggct tgttctgtga gaataccggt tgctcctttg
     3061 tggggaatga ttgacattgt gatggagaat tgatcagatg ggaacattga tttcagaaag
     3121 tcttcattca attcctttgt ttgtctacct tttcctgaaa ttggatttac gataaaatga
     3181 atatttctca tataagaaca acagacaata agtactttat attttgttta tgtattggtc
     3241 atctctaatt tacctaaaaa ttttaaaaca gggcccatca tcgatgatat attccggata
     3301 aaaatatcct ggatggcagc ataccgattt gcgttttcgt tatttgataa aggagtggaa
     3361 atgtattcac aaggatattc atgaaaccag tcgttggtaa gcgagttccc tccattgttt
     3421 ttgaaaatct ttaggttaat agatatcatt gaatgaaagg taggcaaaag tgtctgcctt
     3481 ttattatttg gctttgaacc atcttaccat gtctttgaat gttgcctttt tgccgtacat
     3541 caatattcct acacgatata tccttgctga aagccaggca aaaaaccagg atgacaatgc
     3601 cagggatatc agactggtca ctatctgcca aacaggagga tcaaatgcca gtctgacggg
     3661 cattacaatg ggtgaaaata aaggtatgat gctgctccat actgccattg agctattggg
     3721 tgaactgatg gcagccatgg agatatagaa accaaataac aaaggcatga tgacgagaaa
     3781 ggtaaggctc tggccttcgc cctgatcatc acccatagta gaacctattg cagcaaacat
     3841 ggaagaatac atcaggtaac ccagcaggaa aaacaaaaaa atagtcggaa tcagtaccca
     3901 ccattgcact gcctgcaatt gaccgataat atctcccata aaggaattgg cgacctgtgt
     3961 ctggacaact tctccggtcg cgcccgtagc catcatgcct gacatgtcca tatcacctgc
     4021 aaacaggaag gtgatcagaa agatgaagag gggagtagtg atacaccata tcaggatctg
     4081 aaacaagccc accagcccta caccgataat ctttccgaac atcaattgtt tgggagtcac
     4141 agtgctgatc ataacttcga taattcggtt agtcttctct tccatcacgc ttctcataat
     4201 catcgaccca ttgatggtta atagtaaata aagaaagaat ccgaagatac ctccgatgac
     4261 agcagccact tcgcttccac tttccttctt tccgctgata ttttcttcca gcaagtgaat
     4321 aggttgatcg atgtcgtcca atttgttctt atccagacct aatagttcca gttttttgtt
     4381 cttgatactt acccgaataa atttttcaat ggatgccttg gtttggagat ccaatctctt
     4441 tacggtccac attgcaggtg agtattgctc cgacaaattg gaggaaattt caggtagaac
     4501 gatggccgct ttgaagtttc cttccagctc ctttttaatg acctcttcat tatagtcaat
     4561 ccgggtaaat gtcaggttgt cccagctgtc agagctttcg gggatgattt tgttggggtc
     4621 gacaagaagg atgggagttc cggaattgcc tttgtaagac atgataaagc ccactgagcc
     4681 ggaaaagatg gctatgatta aaggcatgaa gagggttatt aaaagaaatt tcttcttcaa
     4741 tgcacgagtg gcgaattctc ttttagccac gatgagggtt ttattcataa cttcaatatt
     4801 atgttctatg acgtcctttt gtaaaaatgt gccaaattac gatgatgtat ttaattaaat
     4861 atatgaaaga ttattttatc tttgcgcaac ttttttaaaa aaatcgtaaa aaccctagag
     4921 gataacgtac ttatggaatt taacattaat gcattaagaa catttgttga ccagaaaaaa
     4981 aatctagtta tcggcatttt ggccggcttg gctattttga ttgccggata ttttatttat
     5041 aaggcatttt ttgtagctcc aaagaataaa gtggctgcgg accagattta tcaagctcag
     5101 atcatgtttg ataaggattc atttcagatg gcgctgaaca atccgggtgt tggcggtacc
     5161 ggtttcctca agattattga gaactatggc gggactgaaa cgggcaattt ggcaagatac
     5221 tatgccgggg tctgttacct tcgtttgggc gactatgata aagccatcaa atatatgaat
     5281 gagtataagc cggccggtga gattacacct gctatgacct atgaagtgct tgctgatgct
     5341 tattctgaga agaaagatga caataaggca ttgagttatt atgagaaagc tgccaagagt
     5401 gcagataatg aactgaccgg tccctatacc ctgaaaaaac tgggtatgtt ccaggagaaa
     5461 ctgggcaaat atgccgacgc tgccaagtca tataagaaga ttatggatgt ctatggtcag
     5521 acagcagatg caaataatat cgaagcctat tacgaaagag cgatgttgct ggcaaataag
     5581 aagtaaaaca atagtcatat aatcccagaa acaaaatccg ggtattgcct ttttgagggt
     5641 agtatccgga ttttttttga tctgtttttg tgctgctttg tagctgactt cgtcacaata
     5701 aaccgtcagt ttttgtatac cattgtatat cagataggta gtaaattggg caaaaaattt
     5761 ttgggtgtcc caatgtgaga gtcagaaaaa aatggctcta tatggaatag cgtgggtaat
     5821 caattttaag tttggcggaa gtaaagtaga tcatattgat aaagttgttg atattgcggt
     5881 aacctcatac tcgtgtttgt gctaattgaa ttttactgtt tatactttct atgattccga
     5941 tattaattcg actttgaagg tagtctccaa tgccactcca aagagctttt atggaatatc
     6001 catctttgat aaagagatat atcccaattt tcaaaacaag gttgtatttg tttatactcc
     6061 aaagttacgt tacccataca aattcataaa gggccaaaaa gaaactcagt tgtcccaaaa
     6121 ctgattgacc aaaaaaaatg tgtacttttg tccttgtaat ttatgttgtg atagccctaa
     6181 attacatcat gacgggaaat ctgaaaaatg attcctgttt taatttttat cggacaatag
     6241 tgattaataa ttatggttat cgaaacattt tttctcaata aaatacttta agcgatgaga
     6301 caattttata tagctttatt tttattagta actgtttgct taaaagggca agatatcaaa
     6361 tttggtgtag aagccggttt taactctgta aaatctaaat taccaaaaga gtataattct
     6421 tattttaaac aaaaaccttc tttcatcata ggaatccctg ttgatatttc attcactaaa
     6481 cttttcaaca ttcatagtgg actttatttt tctcaattag gaacaaaagc gcattatctt
     6541 caagataaca gaccgtattc atactcagaa tcttatcgta atggaacaat aaattcaata
     6601 atcatacctg caaattattt ttatgaattt aaaatcaata atatcggatt gtttggcggg
     6661 ataggtttcg aaggagagtt caatatatct ggaaaatctg ttggttatga catccaagag
     6721 tcctcaattc catggttcga taattatcat aaagattttg aaaaaaaagc aaaaatagga
     6781 aaagatctgc ctctcattgc attcggattt aatgtttatg gaggagtgaa atttaaggat
     6841 gcttttttcg taaaattatt tcttacccaa ggactatcaa atatagatat cacaccatcg
     6901 tacagttcag ttcctgaaaa acaacaacaa tttggtttaa ctatagggta tatgtttcaa
     6961 aagtaatatg aggcttaaac ctacaataaa atttagactt tctatccggt aaaataaaat
     7021 taatttattg gaataagttt ttgtaacaag gattggaaat cttctatttt atctcctaag
     7081 attctacatg aaagagaata ggatgataaa aattataaaa attaaaactt caacgttgta
     7141 attttagatc tttattacca ttctatgaaa tgtccgtttt tgatttgtac cgataagagt
     7201 cagattaaat agtagctact acaatttatt atttcatttt agtatacctc gcatcatcca
     7261 gacactatcc attaagcatt gaatacaacc tcctctttct aagaggaact tgatatcggt
     7321 tctttgtaaa caggttattt ttcttcttcg aagaattgat cgtagtcgtt atttttcttt
     7381 tccctttcga cataggctct ttcagcacgg ggagcattta agtccaacgt tgtactttcg
     7441 ctttttcttc caccggcgag catcagagta ctttctttct cccattgctc aagcatcttc
     7501 ataccatctt cttcaaatac ggcgttttgc aagtcaatgg aatccatgaa gttggaagac
     7561 atttccataa atcgctccat ttcgccaacc ttcattgcca catcgtctgc aatggattcc
     7621 atagcctgat cgaacatggc acgcttgtca gaattgccgg cgatgatatt cattgcggat
     7681 ttcatagcac tgtgtgaggc tcggatggcg gcacgctctt gttccttaac cttgacctgg
     7741 tcacgggtgt cttccagaag gatttcggaa ttgctgtaca tctttgtcag aattctgtaa
     7801 agcacttcca tcttttgatg aagtacctga tatttttcat tggtatctgt aaggcgagcg
     7861 gcttttcggg aagaaagaat catatgctga tccttatcca tctccttggc cttacttgcc
     7921 attttcagat tgatatcgat ggaagcctta ttttcatcca tgatattctg aagtttacgc
     7981 atttgacctt tgagattacc gatctgagta cccattttct tcaggttatt ttccagatct
     8041 tcgatatagc ttttcagaat acctatggga tctatctgta cgaaccaacc ggtaattttc
     8101 cgcatcacgc ttttgtacat ataccatatg aggttgcgca tcttgggatc gaagaccatg
     8161 tagagcaacg cacccaaaac gatgaccatt ccggcaaggt aaagggtgtt ttttgccagc
     8221 tcgatgaggt aaggcagggc tttgtataac aagtatccgg cacctgcaag aattcctgcg
     8281 cccaccaata atcctgcctt gccctcaggt ctttgaataa atgattttgg cttaccatcg
     8341 gcaccagtaa agggagtaat ttgctgtgtc atttcctcag atattttgat tgattaattc
     8401 aatgtccttt gatagttgcg tgataatctc agaaaaagta tgttcgaatt tgtaccttga
     8461 atcttcgatt ttggctattg cagcttcaat ttcttcgtct acaccgtcaa tactcttttg
     8521 atattggtcg atttcggctt gaagcttatt gatcaggtct ttgttgcgac ttatttgatc
     8581 ctgtagtttt tgctgttctg ccttacgccc atcaatcttt tgttcacgct ggtttttcaa
     8641 ggcggcatca aactgtacct tttccttgtg gatgacctgt ttgtagaagt cagcacttct
     8701 cagcagggat tctttggtaa gtcccaatgt attgcctgtt gtaaatgccg acttaaccga
     8761 cattacttcg tccagaggta attgaagcaa agcattgaaa gcttgtttga attcgatata
     8821 gtcaaagccc tcctgattgt ttttttcgag agccatcatc ataaattcca ggcttttttg
     8881 atctgcttcg ggtgacagtt taaacaagtt ggatagttga ttcaattttc ccgacatctt
     8941 ctgttaaatg tttttgtaaa aataaacaag attttgaaag tacaataatt aataagattt
     9001 aacatctaac tttcccgatt aattccatag ctcaggctat taaactttcc ctattgaaaa
     9061 atggacgata ttgaagctat tattaatgtc cgggcaataa ttgcggattt tttattagtc
     9121 tgtttcttta tttgccaaaa taaattacct tagcgccgat aattttgaaa taagattcgt
     9181 taaacaaaac atgttataat atttatgagt acaaacgctg catcgatgag tcatcctaaa
     9241 ggcctttacc tgttgttttt cacggagatg tgggaaaggt ttagttatta cggcatgcgc
     9301 gccatttttg ttctttacat ggtcaatggc ctgaaaatgg cggaaaatga tgcttcaaat
     9361 atttatggaa gttatacagg tttggtttat ttaacacctt tattgggtgg atatcttagc
     9421 gatcgttttt taggtaacag gcgaagtatt gaaatcggtg gtatattgat ggctatcggt
     9481 cagtttctga tgttttttag tgcatcgacc tccggggcta ctgcaattac gcttatgtgg
     9541 gcgggcctga cggcattgat aataggaaat ggtttcttca aacccaatat ctccaccatg
     9601 gtaggccagc tatatcctga aggagacaga agagtggata gtgcatttac cattttctat
     9661 atgggtatca atctcggagc cttcttttct ccattggtat gtggaacatt gggcgaaaag
     9721 attgccttca aatggggctt ctttgcggcg ggaatcggta tgttaatcgg agtactgctg
     9781 tttttggcac agaaaaacaa atacctggtg acagataagg gcgaaccaat cggtatgccg
     9841 agcaaaaagc tggacacaaa gaacattgct tatctggttg gttccatact cttgattttc
     9901 ttcctcctga acttcaagag catgttcaat gtggatactg atatcatagg atattttatc
     9961 tttggctcga tgatcatcgt acctgctatc attataagtg ataagtcgct tacatcaacg
    10021 gagaaaagca ggatctgggt aatttttatc cttgcgtttt ttgtgatttt cttttgggga
    10081 gcgtttgagc aggcaggtgc gtcactcaca atctttgccg ataagcagac cgatcgtcat
    10141 atatttggct gggagatgcc ggcaagttac tttcagtcag tcaatccgct ttccatcatt
    10201 gtactcgctc ctttgttctc cgctatgtgg attaagcttg gcaacaggaa tatggagccg
    10261 gccacaccac gtaaaatggc attgggcctt tccctgattg ccttaggcta tgtcctaatc
    10321 gcctttgcgg tcaaaggtgt tgatgcgagt acaaagatca atatgtttag gttgttcagc
    10381 ctatatgtgg ttcataccat gggtgaattg tgtttgtcgc cgataggact ttctatggtc
    10441 gccaaacttt caccggccag attctcttct ttgttgatgg gaacctggtt ccttgccaat
    10501 gctgcagcca ataaatttgc cggtacgttg agcgaattga ttccgagtga gagcaatcc
//
LOCUS       DAOQQN010000059        10545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2281_length_10545_cov_4.951478, whole genome shotgun sequence.
ACCESSION   DAOQQN010000059 DAOQQN010000000
VERSION     DAOQQN010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10545
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2281_length_10545_cov_4.951478"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..830
                     /gene="pyrR"
                     /locus_tag="PLG24_03575"
     CDS             273..830
                     /gene="pyrR"
                     /locus_tag="PLG24_03575"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HMZ23996.1"
                     /translation="MEEGRELISHRKFDIVTRRLCHELVENYQDFSDACIVGLQPRGT
                     ILSDMISHILQTEMGISNIEYGILDITFYRDDYRDRTKVLRASETRMDFIVERKKVLL
                     VDDVLYTGRSIHAAMSALQHYGRPAHIELLSFVDRRFNRDFPIQANYVGLTVDALDDA
                     YVKLRWNESKNSASVFIYPFKSDTQ"
     gene            827..1762
                     /locus_tag="PLG24_03580"
     CDS             827..1762
                     /locus_tag="PLG24_03580"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HMZ23997.1"
                     /translation="MSEVSSQLSTKHLLGIKDLLVEDIELIFSTARNFKEVINRPVKK
                     VPSLRDITVANLFFENSTRTRISFELAEKRLSADIINFSASASSVTKGETLLDTVNNI
                     LSMKVDMIVMRHPAPGACIFLANKIDASILNAGDGTHEHPTQALLDAFSMMEQIGDLK
                     GRNIAIVGDIIHSRVALSNIFCLKKLGAKVRVCGPPTLIPRYISELGVEVSYDLEDTL
                     KWCDVANVLRIQLERQNTKYFPSLREYSRYFGINRQILEKVNRNIVIMHPGPINRGVE
                     LTSDVADSPASIILQQVENGVAIRMAALFLLATRV"
     gene            1839..3269
                     /locus_tag="PLG24_03585"
     CDS             1839..3269
                     /locus_tag="PLG24_03585"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HMZ23998.1"
                     /translation="MSVFRTGLLLSFILLANSVFAQSGYKIEIKINNYKNDTLLLGYY
                     LGDRQYVKDTAISKKGVFSFTGKNDLDPGVYMAVLLPEKNVFQFVVDKDNRFFSIEAD
                     GANLSKKLKVKNSDENTIFFNYIHFLTSQRPMADSLRSKQQIIQDPVEKQKLAEKIDE
                     IDQRVYKMQDDYVSKYPQSFTAKLIQTSKEQFIPKYGQNDDMETKLRQYNKYKDHYLD
                     NMDFSDERLFRSPVYFQKIDNYIQNITLQQPDSLIKALDIVLAKVKVIPDAFKFYLSH
                     YFNFFIKSKFVGMDAVYVHLIDQYYSKGLAPWVDKENLQKMVDAANDARPTLVGRIAP
                     NLTFINQKDSMDIRLHDIVSPYTVMFFWAPDCSHCEKAVPIVLDFYEKYKDKGVKVFA
                     VCTGLLDEAKDCWKNVVKKHMEPFVNVYDPVLLSNFKVIYNIKSTPQFFILDKDKKIL
                     SKGIGADQLNEVMGKIMEFDKNNHQQ"
     gene            complement(3297..4511)
                     /locus_tag="PLG24_03590"
     CDS             complement(3297..4511)
                     /locus_tag="PLG24_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ23999.1"
                     /translation="MKLGILREGKIPHDDRVALTPAQCRILMGDPHNFSIVVQPSPTR
                     CFKDEEYIAAGIPVQEGLDDCNIILGVKEVPIKDLMQDKTYFFFSHTIKKQPHNRKLL
                     QAILEKRITMIDYETLEDSNGKRLIAFGKYAGIVGAHNGIWTYGKRTGLIDWPRLKDL
                     HDYEEAKDVYSSTSLPPFRIALTGSGRVAMGAISALDEMGIREVSPEEYLKNISYDHP
                     VYTHLSSQEYVRHKEGREFEKKDFYTNPQNYESAFEDYAEVTDIFLNCIFWNIHSPAF
                     FSLDDISNRKFRISVIADITCDIAPASSIPTTYRASTIDDPVYGIDRLTFAETAPYQE
                     NSIDIMAIDNLPNELPRDASKYFGSKFVKVIIPELEAEHSDIIQQATIAKDGKLMPRF
                     KYLEDYIATTNV"
     gene            complement(4530..5099)
                     /locus_tag="PLG24_03595"
     CDS             complement(4530..5099)
                     /locus_tag="PLG24_03595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HMZ24000.1"
                     /translation="MVITIISGSPSKNSVSIRIGWYLMHYIKTLASEVEVNILDCRKY
                     EVPLDDATYKNDGSAPKHLQPLVELFFRTDGFILLSPEYNGSYTPSLKNLLNHFPKLD
                     HKACGIVTASDGALGGMRAAQQLQQLIAAHFGIISPYMLIVPHVGGKFNEEAELTDET
                     FSRHIIRFVDEYLWLFKALFDSRKKAFKN"
     gene            complement(5131..6168)
                     /locus_tag="PLG24_03600"
     CDS             complement(5131..6168)
                     /locus_tag="PLG24_03600"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24001.1"
                     /translation="MQKTVFFLIFCCIGLTSTAFSQNQYKVINIGFYNLENLFDTLDT
                     PKKMDEEFTPTGSRLYNTAVYLDKLTRLADVISLLGKEISPDGVAILGLAEIENRKVL
                     EDLTAHPKLKSSDYKIVHYESEDFRGIDVGLLYQPKYLTVLSSKPINVPILNDKGEKV
                     YTRQTLYVAGIMDGDTVHFLVNHWPSRRGGTSSVPLRMQAATIARTVKDSILTISPNA
                     KVIVMGDLNDDPTDKSITVGLKAFGKREKIKPDQMYNPYVDFYRKGIGTTPFQDAWSL
                     FDQVIITPGWLDNSNHGYIYHKARVFNEKFLTESSGKYKGYPKRTFSGDTYNFGYSDH
                     FPVYLTFVKKI"
     gene            complement(6225..6755)
                     /locus_tag="PLG24_03605"
     CDS             complement(6225..6755)
                     /locus_tag="PLG24_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24002.1"
                     /translation="MSTEPKEYLQKMYSETLQASRFNTFRIFNTLIIALFVLLFMNVL
                     FTTPDNTFSYITLIIAVVSVLFMFYRLTRAKAGVKALPQDVSDIDGEIIRQHIDAIRI
                     SQRKLRWQMAVFTIPFFIYFLIDLVVSSTEIVEKKYLIMKIAFFFVLLILYYYLFFKI
                     RDTNFEERNSTKTQAD"
     gene            complement(6952..8109)
                     /locus_tag="PLG24_03610"
     CDS             complement(6952..8109)
                     /locus_tag="PLG24_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HMZ24003.1"
                     /translation="MKQFLSLLFAGLIGGAAAVGGIKYYESQSAPNIPEITETATAHQ
                     VNYAPVNVPFDFTQAAGKSLDAVVHIKAAESRQSAYQRQLQQQRTDPFSFFFGQRGPQ
                     QGSGSGVIISEDGYIVTNNHVVDFADQLEVTLHDGRVYEAVKVGTYPNADIAVIKIKA
                     NDLKPIKYGNSDDVKVGEWALAVGNPFDLTSTVTAGIISAKGRSIDIIHTKDAVENFI
                     QTDAAVNPGNSGGALVDINGNLIGINTAIATPTGTYAGYAFAIPVNQIIPIVENIIKN
                     KGDYNGPNLGAKATLGVTVANVTDLTPDQKEENGVIAKTGVLLLELQNGGSAQYSGLL
                     PNDVITGINNKAINNINDLKSTLSKLKPDDVISVSFKRGDQTKQLDVKLRS"
     gene            complement(8236..8958)
                     /locus_tag="PLG24_03615"
     CDS             complement(8236..8958)
                     /locus_tag="PLG24_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HMZ24004.1"
                     /translation="MSDHREVQPIKKERSPFPQKSDFFTAISVDCVIFGLDRESLKVL
                     LIKSDFPLYEGKWSLLGDLIAKNEDINQAADRVLYERTGLQNLYLEQVMSFGEVKRHP
                     SGRVVSIAYYTLVNIDQINLLKKDHELHWHKIDQVGEMAFDHNKILNICLSRLQERIK
                     SEPIGFNLLPQKFSLRELQLLYESVLMVELDRRNFRKKMLSNGLLIDLKELEDDVPHR
                     PAKLFSFNRQKYDLTEIKGKFI"
     gene            complement(8960..10432)
                     /locus_tag="PLG24_03620"
     CDS             complement(8960..10432)
                     /locus_tag="PLG24_03620"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HMZ24005.1"
                     /translation="MENNLSFKERNEHTLTEWRNKEMQALDLLQKAGELRFDRSVELV
                     LFRKDIYDTRPSELLNLHQNAANYGETEVTIEDTVAITNELVAMEDLRQARLDIGSLA
                     LEWKNNKEKYSSLGEFIGARLKTAQGAQSENIMPRDVILYGFGRIGRLVARRLVSQTG
                     RGEQLRLKAIVIRPQMKDRKQDAEKRAALLRTDSVHGEFAGSVRVSDDGSELIINGNR
                     VLLIYASYPSEIDYSALGINNALVIDNTGVFRTKEDLEQHLRPGVDHVILTAPGKKIP
                     NIVYGVNHESIDFNNDKVLCGASCTTNAIVPPIKVLDDAFGIEKGHVETIHSYTSDQN
                     LLDNFHKKPRRGRAAAINMVITSTGAAEAITAVLPYLDGKITGNAVRVPTPNVSLAII
                     NVSFGRPTTVDEVNNLLKQASLSGPLAEQIGFSNDPEYVSTNGVGMTTACVIDGPSTI
                     VSNDGKTVTVYAWYDNEFGYTCQVVRVAKHVGQVRRKTYY"
BASE COUNT         2981 a         2178 c         2062 g         3324 t
ORIGIN      
        1 agtaaatcat ctgaattttg gcaaagccgc cccgaaaggc aataccgtca agcattgtgg
       61 ctattaatta tctacctact tcagcttccc acctacccct ttcaccctca ttaccctact
      121 tccactacct ccccacagac ttcatgcttt tttcttttct tttctcttcc actccttttc
      181 atttttcatc gtcataatgt gtactatcac tttgtattca aaatgaattt tccaccaatt
      241 ttttaacgga atcaacttat ctttgcaggg aaatggaaga aggtagagaa ctgatttctc
      301 ataggaagtt tgacatagtc acaagaagac tatgtcatga acttgttgag aattatcaag
      361 acttttcaga tgcatgcatt gtaggcctgc aaccacgtgg tacaatcctt tctgatatga
      421 tcagccatat cctgcaaacc gaaatgggca tttcgaacat tgagtatggc attctggata
      481 ttacttttta tagagatgat tatagggaca ggacgaaggt cttaagagca agcgaaacac
      541 gtatggactt cattgttgaa cgaaaaaaag tattgcttgt cgatgatgtc ttatacacag
      601 gccgctccat ccatgccgca atgtctgcac tgcaacacta cggcagacca gcccatatag
      661 aattactttc atttgtcgat cgcagattta acagggattt tcctattcag gccaattatg
      721 tgggactcac tgttgatgca ttggatgatg catatgttaa attgagatgg aatgaatcca
      781 aaaactctgc ttcagtattt atatatccct ttaaatccga tacccaatga gtgaagtgag
      841 tagccagttg agtaccaaac atttacttgg aatcaaagac ctgctggtgg aagatattga
      901 actcatcttt tctactgcaa ggaattttaa ggaagtcatc aatagacccg tgaaaaaagt
      961 gccatcctta agggatatca cggtagcaaa tcttttcttt gaaaacagca ccagaacaag
     1021 gatttctttt gaattggcag aaaaacgtct ttctgctgat atcatcaatt tttctgcatc
     1081 agcatcttca gttaccaaag gtgagacctt gctcgatacg gtaaataaca ttttgtccat
     1141 gaaggtggac atgattgtca tgcgccatcc tgcacctggt gcttgtattt ttcttgccaa
     1201 taaaatcgat gcttcgatac tgaatgcggg tgatggaacc catgaacatc ccacccaggc
     1261 tttgcttgat gcattttcca tgatggaaca aattggtgac ctcaaaggac gcaatatcgc
     1321 cattgtcggt gatatcattc atagcagggt agcattgagt aacattttct gcctgaaaaa
     1381 attaggtgcc aaagtccggg tttgtgggcc tccaacactg atcccacgat atatctccga
     1441 attgggagtc gaagtgtcct atgacttaga ggatacactt aaatggtgtg atgtcgctaa
     1501 tgtgctcagg attcaattag aacggcagaa taccaaatat tttccttcct tgcgtgaata
     1561 ttcaagatac tttggaatca atcgccagat tcttgagaaa gtaaatagaa acatcgtcat
     1621 tatgcacccc ggcccaatta atagaggtgt tgaattgacc agcgatgttg ctgatagccc
     1681 tgcatctatt atcttacaac aggttgaaaa tggtgtcgct atccgtatgg cagcgctctt
     1741 cctgcttgct accagagtct gaccttattt ttgcaaaaca ttaagaacta atttcgctgc
     1801 caatgcgtta ctttacatct gttttaatag taataaatat gtcagtgttt cgtacaggtc
     1861 tattgttatc ctttattctt ttagcgaatt cagttttcgc acagtccggt tataagatcg
     1921 aaatcaagat aaataattat aaaaatgata ctttattgct gggttattat ctgggagaca
     1981 ggcaatatgt caaggataca gcaataagca agaagggtgt cttttctttt acaggaaaaa
     2041 atgatttgga tcccggtgtg tatatggctg ttttattgcc tgagaaaaat gttttccagt
     2101 ttgtagtgga taaggataat cgatttttca gtatagaggc tgacggagca aatctttcaa
     2161 aaaaattgaa ggtaaagaac agtgacgaaa atactatatt tttcaattat atccattttc
     2221 ttacctccca aaggcccatg gcagattccc ttcgttcaaa acagcagatt attcaagatc
     2281 ctgtagaaaa gcaaaaactg gcagaaaaga tagatgaaat tgatcaaaga gtatataaga
     2341 tgcaggatga ttatgtttct aagtatccgc aatcctttac tgccaaatta attcaaacat
     2401 ccaaggaaca gtttattcca aaatacggac agaatgatga tatggagacc aagctccgac
     2461 aatacaataa atacaaggat cattatttgg acaatatgga tttcagcgat gaaagactct
     2521 tccgatcccc tgtatatttt cagaaaattg ataattatat ccagaatatt acattgcaac
     2581 agcctgattc tctgattaag gctttggata ttgttttggc taaggtcaag gtcattccgg
     2641 atgcattcaa attttatttg agccattatt tcaacttttt tatcaaaagt aaatttgtcg
     2701 gaatggatgc cgtatatgtc catcttatcg accaatacta tagtaaagga cttgctccgt
     2761 gggtggataa agaaaacttg caaaagatgg tagatgcggc caatgatgcc agaccgacac
     2821 tggtaggcag aattgcacct aacctgactt ttatcaatca aaaagacagt atggacatca
     2881 gattgcatga tatagtgtct ccttatacag tcatgttttt ctgggcaccc gattgttcgc
     2941 actgtgagaa agctgttccc attgtcctgg atttctatga aaaatataag gataaaggcg
     3001 taaaagtctt tgcagtatgt accggactat tggatgaggc gaaagattgc tggaagaatg
     3061 tggtgaaaaa acacatggag cctttcgtca atgtctacga tccggtgttg ctttccaatt
     3121 ttaaggttat ctataatatc aaatcaacac cccaattctt catcctcgat aaagataaaa
     3181 agattctttc caaaggaatt ggtgccgacc agctgaatga ggttatgggt aaaattatgg
     3241 agtttgataa aaataaccac caacaataga atttccggct ttccaattat accggcctat
     3301 acattagtcg tggctatata atcctccaga tatttaaacc ggggcattag tttaccatct
     3361 tttgctatag ttgcttgttg tatgatgtca gaatgctcag cttcaagctc aggaattatg
     3421 actttcacaa atttactgcc gaaatacttg gatgcatccc ggggcaattc attgggtaga
     3481 ttatcgattg ccattatatc tatactattt tcctgatatg gcgctgtctc ggcaaatgtc
     3541 aaacggtcaa tgccatacac gggatcatcg atggtacttg cccggtatgt tgtgggaatg
     3601 gaagatgccg gagcgatgtc acaagttatg tccgcaataa ctgaaatccg aaattttctg
     3661 ttcgaaatgt catccagact gaagaaagcc ggtgaatgaa tgttccaaaa aatacagttt
     3721 aggaaaatgt cagtcacttc agcataatct tcaaaagcag attcgtagtt ttgaggattg
     3781 gtgtaaaaat cctttttttc gaactcacga ccttccttat gcctgacata ttcctgtgag
     3841 gatagatgtg tatataccgg atgatcgtaa cttatatttt tcagatattc ttccggtgat
     3901 acttcacgga tgcccatttc gtccagggct gaaatagctc ccatcgctac tctgccacta
     3961 cccgtcaagg ctattctgaa tggaggtagg gaggtcgatg aatagacgtc cttagcttct
     4021 tcataatcat gtagatcctt aaggcgcggc caatctatca atccggttct ttttccataa
     4081 gtccatattc cattgtgagc ccctactatt cctgcatatt ttccgaaggc gatcagcctt
     4141 tttccattgc tgtcttcaag agtttcataa tctatcatgg taattctttt ttcaagaatt
     4201 gcctgaagta attttctgtt atgaggttgc tttttgatgg tatgcgaaaa gaaaaaatag
     4261 gtcttatcct gcatcaaatc ctttattgga acttccttta ctccaagtat gatgttacaa
     4321 tcgtccagac cttcctgaac cgggattcct gcagcaatat attcttcatc tttaaagcat
     4381 cttgtaggtg agggctgtac gacaatgctg aaattatgag gatctcccat caatatcctg
     4441 cactgggcag gagtcaatgc tactctgtca tcatgcggta ttttcccttc tctaagtatt
     4501 cctaacttca tgtatttttt cgtttcctgt taatttttaa atgccttttt ccttgaatca
     4561 aataatgcct tgaaaagcca aagatattca tcgacgaatc tgataatatg ccttgaaaaa
     4621 gtctcgtctg tcaattccgc ttcttcattg aattttcccc cgacatgagg cacgatgagc
     4681 atatagggag agatgattcc gaaatgagct gcaatgagct gctgcaactg ctgagctgca
     4741 cgcattcctc ccaaagcacc atcagaagct gttactatac cacaggcctt atgatccagc
     4801 ttaggaaaat gattgagaag attttttagc gatggagtat agctcccatt atattcaggt
     4861 gaaagtaaaa tgaatccgtc cgtcctgaaa aataactcta caagaggttg taaatgcttt
     4921 ggagcgcttc catcgttttt gtatgttgca tcatccagtg ggacttcata tttccgacaa
     4981 tcaagtatat tgacctcaac ttcagatgcc aaagtcttga tgtaatgcat caaataccaa
     5041 cctatgcgta ttgaaacgct gttttttgaa ggactccctg aaatgattgt tatgaccact
     5101 tggtattatt gaaccacaaa tgtaatggtc ttatattttt tttacaaaag ttaaataaac
     5161 cgggaagtgg tcactatatc caaaattgta tgtatctccg gaaaaagtac gcttaggata
     5221 acccttgtat ttgcctgaac tttcagtcaa gaatttttcg ttgaatacac gagctttgtg
     5281 atagatatat ccatggttgg aattatcaag ccatccggga gtaatgatga cctggtcaaa
     5341 cagactccag gcatcctgaa atggagttgt gccaattccc tttctataaa aatcaacata
     5401 tggattatac atctggtcag gcttaatttt ttcacgtttt ccaaaggctt tcagcccgac
     5461 agtaattgat ttgtccgttg gatcatcatt gaggtcaccc atgactataa ccttggcatt
     5521 tgggcttata gtcagaatag aatctttgac agtacgcgct attgtggcag cttgcattct
     5581 cagtggcacg gatgaagtgc ctcctcgcct ggaaggccag tgattgacca gaaaatggac
     5641 tgtatcgcca tccatgattc ctgcaacata caaggtctgc ctggtatata ctttttctcc
     5701 tttgtcgttc aatattggta catttatagg cttactgctt agcacagtga ggtattttgg
     5761 ttggtataga agaccaacat caatacccct gaaatcctct gattcataat gaactatctt
     5821 gtagtccgat gactttagct taggatgtgc ggtgaggtct tcaagtacct ttctgttttc
     5881 aatttcagcc aggcctagta tagcgacacc atcaggactt atttccttgc ccaacagaga
     5941 tatcacatcc gcaagccgtg ttagtttgtc gagatagacg gctgtattgt ataacctgct
     6001 tcctgtcgga gtaaactctt catccatttt ttttggcgta tccaatgtat cgaagagatt
     6061 ctccagatta taaaatccaa tattgattac tttgtattga ttttggctga atgcagtgga
     6121 agtaagccct atgcagcaaa agatcagaaa aaagacagtt ttttgcaaaa ttccgttttt
     6181 ggttattgac aatatttaag tgtgatttaa gtggatggaa gaaattaatc tgcttgtgtt
     6241 ttggttgaat tgcgctcctc aaaattagtg tcgcggatct taaaaaacaa atagtaataa
     6301 agaatcagca aaacaaagaa aaaggcaatc ttcataatca gatatttctt ctctacgatt
     6361 tcagtggatg aaactacaag gtcaatcaga aaatatataa aaaagggtat ggtaaagact
     6421 gccatttgcc atctcaattt tctttggctg attcggattg catcaatgtg ttggcggatg
     6481 atttcgccat cgatatcgct tacatcctga ggtaatgctt tgactccggc ttttgctctc
     6541 gttaatctgt aaaacatgaa gagtacagat accacagcta tgatgagggt aatgtagctg
     6601 aaggtattat cgggtgtggt gaacaggacg ttcataaaaa gtaatacgaa caaggctatg
     6661 ataagcgtat tgaatatcct gaaggtatta aatctgctgg cttgaagcgt ctcggaatac
     6721 atcttttgaa gatactcttt gggctcggtg gacataatga tgcgtatttt gcatgacaaa
     6781 attagtgaat ttcaatcaca ttgaaaaaca aatatctggg tcttccatat taacctgttt
     6841 ccatgtttta ttgataaaaa aaactgcctg aggcaaatgc tccaggcagc tcagatcaaa
     6901 aacaaaacga aaacttattt tacaatcaat ttaatgctga ctttactttt attaagaacg
     6961 taatttaaca tccaattgtt ttgtctggtc ccctctcttg aacgaaactg atatgacatc
     7021 gtccggtttt aatttagata atgtgctttt caggtcattt atattgttga tggccttgtt
     7081 gttaataccg gtgatcacat cattggggag taagccggaa tactgtgcag aaccaccatt
     7141 ttgcaattcg agcaagagta ctcccgtttt agctatgaca ccattctcct ctttttggtc
     7201 gggagttaag tcagtcacat tggcgacagt gacacccaat gttgctttgg cgcccagatt
     7261 aggcccatta taatctcctt tgtttttaat aatattctca acgataggga ttatttggtt
     7321 gacaggtata gcgaaagcat atcctgcgta tgtccctgtt ggagttgcta tggctgtatt
     7381 gattccgatc aggttgccat tgatatccac cagggccccg ccactgttgc cgggattgac
     7441 ggccgcatcg gtctgaataa agttttccac ggcatcttta gtgtggatga tgtctatcga
     7501 ccttccctta gcagaaatga ttccagctgt cactgtagag gtcagatcga atgggttacc
     7561 tactgccaac gcccattcac ctactttaac atcgtctgaa tttccgtact taatgggttt
     7621 gagatcattg gcctttatct tgatgacggc tatgtcggca tttgggtagg tacctacttt
     7681 tacagcttca tatactctac catcatgcaa agtaacttcc aattgatcag caaaatcaac
     7741 cacatggttg ttggtcacaa tatagccatc ttcagaaatg ataactccgg agcccgaacc
     7801 ttgctggggg cctctttgac caaagaaaaa tgaaaaggga tcagttcgtt gctgttgtag
     7861 ctgacgttga tacgcactct gtctgctttc tgctgccttg atgtgcacta cggcatccag
     7921 tgatttgccg gcagcctgtg taaagtcaaa gggtacatta accggtgcat aatttacctg
     7981 atgcgctgtg gcggtctctg ttatttcagg tatatttggt gcactttgag actcataata
     8041 ttttatcccg cccactgctg cagcacctcc gatcaaaccg gcaaacaata atgaaaggaa
     8101 ttgtttcata aattcaataa ttgcttttat aagatttgaa taattttaat tagattattt
     8161 agccatttaa cgccgacaaa ggatttgagg ttaccttcca aggcaactat taacaatatt
     8221 ttaacctgca atctttcaga tgaattttcc ttttatctct gttaggtcat atttctggcg
     8281 attaaaggaa aataacttgg ccggtcggtg aggtacatca tcctccaatt ccttaaggtc
     8341 aatcaatagc ccgtttgaca gcatcttttt gcggaaattc ctcctatcca gttccaccat
     8401 taataccgat tcgtacaata actgcaattc acgaagagaa aatttctggg gcaggagatt
     8461 gaatcctatt ggctcagatt tgatccgctc ctgtaatcgg ctcaggcata tattcagtat
     8521 tttgttatgg tcgaatgcca tctcaccgac ttggtctatc ttgtgccaat gtagttcatg
     8581 atcttttttc aataggttga tctgatcgat attgaccagt gtataatagg ctatggacac
     8641 aacccgaccg gagggatgtc gttttacctc tccaaaactc atgacttgtt caagataaag
     8701 attttgcagc ccggtacgtt cataaagtac acgatccgct gcctgattga tatcttcatt
     8761 ctttgctatc aggtctccga gcaatgacca tttcccttca tacaatggga aatcactttt
     8821 gataagcagt actttaagag attcccgatc aagaccaaat attacacagt ccacagaaat
     8881 ggcagtgaaa aaatccgatt tctgtggaaa aggtgatcgc tcttttttga ttggctggac
     8941 ttctctatga tctgacaaat caataataag tttttcttct cacctgtccg acatgcttag
     9001 ctacccttac aacctgacaa gtatagccga attcattgtc ataccaggca tatacagtga
     9061 cagtctttcc atcatttgac actatggtgg aaggaccgtc tattacacat gcggtggtca
     9121 tgccaacacc attggttgat acatactccg ggtcattgga aaatccaatt tgctctgcta
     9181 acggcccgct cagcgatgcc tgctttagta gattattgac ttcatcaacg gtagttggtc
     9241 tgccaaaact tacgttgatg atggcaagtg atacgttggg tgtgggtacc ctgacagcat
     9301 tgccggtaat ctttccgtcc agatatggaa gtactgccgt tatggcttca gctgcaccgg
     9361 tactggtgat caccatattg attgctgcag cacgtcccct gcgaggcttc ttgtggaagt
     9421 tgtccagcag gttttggtca cttgtatagg aatggatggt ctcaacatgg cccttctcaa
     9481 taccaaacgc atcatccaat actttgatag gtggaacaat ggcattggtg gtgcatgagg
     9541 ccccgcaaag taccttgtca ttgttaaaat caatggattc atgatttaca ccatacacta
     9601 tgttgggaat tttcttaccc ggtgcggtca ggatgacatg atctactccg ggacgaagat
     9661 gctgttccag atcttccttc gttctgaata cgccagtatt gtcaatgacc agggcattat
     9721 tgattcctaa tgctgaataa tcaatctccg atggataact tgcatatatc aacaacactc
     9781 tgttgccatt tattataagt tcgctgccat catcgcttac ccgaactgaa cctgcaaatt
     9841 cgccatgcac ggagtctgtt cgtaaaagag cagcccgttt ttcggcatcc tgcttcctgt
     9901 ccttcatttg aggtctgatc actatagctt tgagacgcaa ttgctctcca cgccctgtct
     9961 gagatactaa cctccttgct accaaccttc cgattcttcc aaacccatac aggattacat
    10021 cacgaggcat aatattctca gattgggcgc cctgtgctgt ttttaatctg gccccgatga
    10081 attcaccgag actagaatat ttttccttgt tgtttttcca ttccagtgca agagaaccta
    10141 tatccagacg agcctgtctg agatcctcca tagccaccaa ttcatttgtt atggccaccg
    10201 tatcctcaat cgtcacttcg gtttctccat aatttgctgc attctggtgt aagttcaaca
    10261 attcactggg gcgggtatca taaatatcct ttctgaacaa aaccagttca acagatctgt
    10321 caaatcttag ttcacctgct ttttgcaaca ggtccaatgc ctgcatttcc ttgttacgcc
    10381 attctgtcag cgtatgttca tttctttctt taaaacttaa attgttttcc atgactcgat
    10441 tgcaatatat gttttttgaa tttgccacaa gatataaaat ttcccgtttc ttaaggcatc
    10501 caataaatta tttcaagctt aaagcgtatt tttcctgctt aaatt
//
LOCUS       DAOQQN010000060        10412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2336_length_10412_cov_5.289466, whole genome shotgun sequence.
ACCESSION   DAOQQN010000060 DAOQQN010000000
VERSION     DAOQQN010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10412
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2336_length_10412_cov_5.289466"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLG24_03625"
     CDS             <1..540
                     /locus_tag="PLG24_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMZ24006.1"
                     /translation="QSLEEIIKACAENDRIGQEQLYRLYFTSMVVMCRNYMTNEEDIL
                     SVVNDGFLKVFVNLTKSPVLTNVEGWIRRIIYNTMIDFHRSLKSYKANISLVDTIFDS
                     TVSQSPDEDTDYIFRLIDKLPDVTKTVFTKYAIEGYSHREISEYLNIRENTSKWHLFE
                     ARKKLREWLGVHYLNNQAG"
     gene            501..1769
                     /locus_tag="PLG24_03630"
     CDS             501..1769
                     /locus_tag="PLG24_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24007.1"
                     /translation="MAWSSLFKQSGRMKGLEERGWEDMLQRLNSTLPTHTKTGGSFRY
                     VAVFVMILIFFSTSSYLTFKHFMLDDTSDTILPGSAAPERLAGHVDLYSSTNGIDLAV
                     KTADESNNKDGIAVSKFAIEANYGVNNASKSARDVSNRINKSLKAHPDYTESISNVSF
                     DGSMENGFTQNTHKEENAGIRTHQEGITKRSSGIEDIEKLLMAETELLSSVEQNSPVI
                     ALKSRSTDRRLAFSSILGAYSDIHGRYFGMDIAPQVDFKISKGTSVFFRLPIRFKHTL
                     DNEIVRDVSNELNHNFEQKNLFLPDNLDIQSGKVNANTMDVNMVLGVSQRLGNKVALS
                     TYGLCGYENVLGLIQAFSSIPEFSSAANIAYRTANTSIAAKRDGWVYGGGVEAEIRLG
                     SRHSLIGGMNYYFNSDKSFEAGLGYRFRIY"
     gene            complement(2223..4175)
                     /gene="dnaG"
                     /locus_tag="PLG24_03635"
     CDS             complement(2223..4175)
                     /gene="dnaG"
                     /locus_tag="PLG24_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HMZ24008.1"
                     /translation="MIPSKTVEEIFQKARIEEVVGDFLVLKRRGVNMIGHCPFHNEKT
                     PSFVVSPGKNIFKCFGCGRSGTPVGFIMEHENMAYGDALRYLAKKYNVTVEEEARTPE
                     QNEVARQRESLLLINEFAMKHYISNLTETEYGRNIGLSYFRERGFHDVTIEKFGLGFA
                     FPDGHNLINEAKIKAFNIDLMKQIGLVTQNDRDFFVNRVMFPIFNLSGRIIGFGGRSL
                     TISEKAPKYLNSIDSDIYNKSKILYGFFQGKNAIRKEDNCYLVEGYTDVISLHQAGIE
                     IAVASSGTALTGEQAQLIKRFTDNVTLLYDGDAAGIKAAQRGLDILLQQNLNVKIAVL
                     PDGHDPDAYLKSHGAQALKDFVTRESRDFILFKIAGVAEEAKNDPIKKVTLAQDILQS
                     IARIPDNLKREVYVNQTAKILDIREEVLQSELNKAVRKFSDQQRLEAIREQRLDNSQQ
                     KEYTNKPSARQDASITPPTDNMPLHSILKVLIIYGNKTFEYDGEELVKDRIFKDIAEE
                     MDYIDHQLYLELLNEYQNREKGGENNSPEYYMNHPRQEIRDMVLRWVDPGYEFSQNWI
                     DKYQHPLQYQLMPDENYRNEYLQAFDLLKMNNLNKLAEDNKNKIKEFIENGDQEGLQV
                     QLQAQILINKERNKLANKLGMVIIPK"
     gene            complement(4312..6504)
                     /locus_tag="PLG24_03640"
     CDS             complement(4312..6504)
                     /locus_tag="PLG24_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HMZ24009.1"
                     /translation="MSLKRSNAIDIIHNRFDIDFPVNTPDKVKISGFYGENVFHDEAM
                     KKYLSESAYFSVKAAIQSGTNLTKEVANSVAEAMKNWAMDKGATHYAHWFQPLTGKTA
                     EKHDSFFTITADGKAIEEFGGEQLVQQEPDGSSFPGGGLRSTFEARGYTSWDPSSPAF
                     IIEVGNGKTLCIPTIFVTYSGESLDYKLPLLKSVAYLENAALEVCKIFDESIERTHAT
                     LGWEQEYFLVDESFLIARPDLMITGRTLLGRQSHKGQQLEDHYFGSIPERVLNYMRDL
                     ETECHKLGIPVRTRHNEVGPGQYELAPMYEEVNIAIDHNQLLMDIMERVAKRHKLAVL
                     LHEKPFNGVNGSGKHNNWSIATNTGKNLLSPGKNPGKDLKFLTFFVNTIRAVKEHADL
                     LRASVASASNDFRLGANEAPPAIISVYVGKALTEVLHFIAEGKESDKTMVKEVLSLLK
                     KSPNLELDNTDRNRTSPFAFTGNKFEIRMVGSSMNCSAPMTIMNTIVGNQLTEFKKEL
                     DLLTSKGESMDTAILSILRKYINESLPILFEGDGYSEEWQKEAEKRGLSNTPNTPDAL
                     KVYKMDKTSELFQKTGIFSEIELEAHYTVMVENYNLKLEIEARVLSDLCKSYVLPACY
                     KYQTGIAENIYTLKQLGLQEDAFRPQSNLLNEITHHINNVAEKVETLNQSVNKAVSEA
                     EPEEEAELFCHLVKPIMAEIRESVDHLEEIVEDGLWPLAKYREMMFIR"
     gene            6743..8791
                     /locus_tag="PLG24_03645"
     CDS             6743..8791
                     /locus_tag="PLG24_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24010.1"
                     /translation="MLILFCLPLEAQVDGSTVDIEVIDEVIESILASNPDLTEAQIND
                     LTDRLSVLAQSKVDLNESDLKVFEELLLLNELQIREILLHREVYGDFIALYELQTVPS
                     LTLSDIRRIWPFLKGIGTPGISPFSWSMLYKGSKTIISRYSRVLEQASGYVGDSSKYL
                     GSRDQISFRMRHVVPGRVSIGIGVEKDAGEPLNTNYNPFLFDFVTFHAYLENVAKQIK
                     QLALGDFQVSAGQGLIMFSGYGFGRNAFTTAVKRNERAIRPSTSLNEINALRGAAVGI
                     ELAPGWQMIAFASLKKSDGTLIIPDSLSGDSDVLISSLQMSGLHRTESEIRNGKHISM
                     FTTGGSLKYNGNRFQFGANAVYDQLDKRVEPADRVYNRFYFKGKNALNASVDYTYYFR
                     NLIFYGETAINPENAVATVNGLLVGLHPKVNLALLFRHLPKEYYSLHGKVFSDQSQTT
                     NETGFYAGLEVKPSPRFLVNIYADFFRHGWPTFSANGPSEGSAYLFKASYQKRKFWDG
                     YLQIRYQNEQVSGGGDALIPPLYFRQKTDLRLHFNKRVSNLMTWSTRLDYNYMKLPGD
                     RKENGYLMYQEFWFKPLGKPYNGFLRFSHFNIDSYQSRIYSYEHYQAYDSRNVAFEGV
                     GNRMVTGIRWKFNNGIVLEGSYNITKYTDRNQIGSGNDRILGPVKSEIRAQIRYGFND
                     "
     gene            complement(8813..9370)
                     /locus_tag="PLG24_03650"
     CDS             complement(8813..9370)
                     /locus_tag="PLG24_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HMZ24011.1"
                     /translation="MKNSLIFGMVIGILLGFAGKLTGQATSRTETKTNARIKWYTIDE
                     AVQQNKIKPRKIIVDLYTDWCGWCKVMDKNTFANDDIINYINKNYYPVKFNAETKDNI
                     EFNNKVYSYVGSKGRGYNEFALLLTGGRLSYPTVVFIDEELNVIQPIQGYQEPKDFYK
                     IMRFFGEDHYKKTPWNVFVQNYSKM"
     gene            10194..>10412
                     /locus_tag="PLG24_03655"
     CDS             10194..>10412
                     /locus_tag="PLG24_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HMZ24012.1"
                     /translation="MKFVKQFFIIFFLVISVVLMIGIFMDKNYHVERTITINTPAYEV
                     FDYIRYLKNLDAYSFWSKIDPEMKRRYEG"
BASE COUNT         2941 a         1997 c         2105 g         3369 t
ORIGIN      
        1 caatctcttg aagaaataat caaggcatgt gctgagaatg accgaattgg ccaggagcag
       61 ctatatcgcc tttatttcac ttcgatggtg gttatgtgca gaaattatat gaccaatgag
      121 gaagatatcc tgagtgttgt caatgacggc tttctgaagg tattcgtcaa tctgaccaaa
      181 tctccggttc ttaccaatgt ggaaggatgg attaggagga ttatatataa taccatgata
      241 gattttcatc ggagtcttaa atcatataag gcgaatattt ctttggtgga tacaatcttt
      301 gacagcactg tgagccagag tcccgatgaa gatactgact atattttcag actaatcgac
      361 aaattgccgg atgtgaccaa aactgttttt accaaatatg cgatagaagg ttattcacat
      421 agagaaattt cggagtattt gaatattcgg gaaaatacga gtaaatggca tctttttgaa
      481 gctaggaaaa agcttcggga atggcttgga gttcactatt taaacaatca ggcaggatga
      541 aaggattgga agaaagggga tgggaagata tgctgcaaag gcttaattcg actttgccga
      601 cacacacaaa gaccggaggc tcctttaggt atgttgctgt tttcgtaatg attttgatat
      661 ttttttcaac aagtagttac ctgactttta agcatttcat gcttgatgat acctcggata
      721 ctattctacc cggttctgct gctcctgaga gattagctgg gcatgttgat ctttattctt
      781 caaccaatgg tatcgatttg gctgtcaaaa ctgctgatga gagtaataac aaagatggga
      841 ttgctgtaag caaatttgcc attgaagcaa attatggtgt aaataatgct tcaaaatcag
      901 cgcgtgatgt ctccaataga ataaacaaga gtctaaaagc acaccccgat tacacagaat
      961 ccatttcgaa tgtttctttt gatgggtcaa tggagaatgg ttttactcaa aatacccaca
     1021 aggaagagaa cgccgggata agaactcacc aagagggcat aacaaaaagg tcttctggta
     1081 ttgaggatat tgagaagttg ctaatggcgg aaactgaatt gttgtcttca gtagaacaga
     1141 attctccggt aatagctttg aaatcaaggt caactgaccg aaggttagct ttttcttcta
     1201 tacttggagc atattcagat attcatggaa ggtattttgg gatggatatt gcgccccagg
     1261 ttgattttaa aataagcaaa ggtacatctg tcttctttag gttgcccata aggtttaaac
     1321 atacacttga taatgaaatt gtgcgggatg taagtaatga gctgaaccat aattttgagc
     1381 agaaaaatct tttcttgccg gataatttgg atatacaatc aggtaaagta aatgccaaca
     1441 ccatggatgt caatatggta ttgggggtaa gccagagact gggaaacaaa gtagccttaa
     1501 gtacctatgg actttgtggc tatgagaacg tcctcggcct tatccaggcc ttttcaagta
     1561 ttcctgaatt ctcctcagct gcaaacatag cctatcgaac tgcgaatacg tctattgcag
     1621 caaaaagaga tggctgggtc tatgggggag gagtcgaagc tgaaatcagg ttaggaagcc
     1681 gacacagcct gattggtggt atgaactatt attttaattc agataaaagc tttgaggccg
     1741 ggttaggcta tcgatttaga atttattgat aatcagccta gtagaaggat tgggtttgcg
     1801 aagggaggag ccgcatataa tgaggaggag tttcctgttg aattaggtag ttgagtaata
     1861 ccgatggact tattattgct cagtactatc tgtttcgact tttgataata ggtcttgaat
     1921 tatgtaattt gtattacaga ttatctcact aatagccggg cgccaagaat ggcaggtatt
     1981 agccaactac ctacttcgat ttttccatat tctacctacc catttccaga tcaattatac
     2041 ccaacccttc cgatacaaat ggcgtaaagg atattatgat gtgaaagtta taccaggatg
     2101 acttatataa tagtccaata aatttgaggt atacttgtcc ggatagttat gcgactagag
     2161 ctctactctg tcccgggagt gaaacaatcc ggggttccgg gatgccgaag gtacattcag
     2221 tattacttcg gaataatcac cattccaagt ttattggcca gcttgtttcg ttccttattg
     2281 atgaggattt gtgcctgcag ttggacttga agaccttctt ggtcgccatt ttcaataaat
     2341 tccttgatct tattcttatt gtcttctgca agtttgttca gattgttcat cttaagaagg
     2401 tcaaatgctt gtaaatattc attccggtaa ttttcatccg gcatcaattg gtattggagt
     2461 ggatgttgat atttatctat ccagttctga ctgaactcat atccgggatc aacccaccta
     2521 agcaccatat ctctgatctc ctgcctggga tggttcatat aatattccgg tgagttattc
     2581 tctccgcctt tttccctatt ttgatattca ttcaataatt caagatataa ctgatgatca
     2641 atataatcca tttcttcagc aatatccttg aagattctgt ccttgaccag ttcttcgcca
     2701 tcatactcaa aggttttgtt gccgtagata atcaatacct tcaatatgga atgcaatggc
     2761 atattgtctg tgggaggggt tatacttgca tcctgtcggg ctgatggctt attggtatat
     2821 tctttttgtt ggctgttgtc caggcgttgc tctctgatcg cctcaaggcg ttgttgatcg
     2881 gagaatttac gtacggcctt gttaagttct gattgcagga cttcctcccg tatatcgagt
     2941 attttagcgg tctggttgac ataaacctcc ctcttgaggt tgtcaggtat ccttgctatg
     3001 gattggagga tgtcctgtgc gagggtaacc tttttgattg gatcattttt agcttcctcg
     3061 gcaacaccgg ctattttaaa aaggatgaag tccctggatt cccgggttac aaaatccttc
     3121 aatgcctgtg ctccatgact cttaaggtag gcatccgggt catggccatc cggcaggacg
     3181 gcaattttca cattgagatt ctgctgcaat aagatgtcga gtccacgctg ggctgcctta
     3241 atgcctgctg catccccatc atataatagg gtcacattat ccgtgaatct tttgattagt
     3301 tgggcctgtt ctcccgtcaa agctgtgccg gaggaggcaa ctgcaatctc aattcctgct
     3361 tggtggagag aaattacatc tgtgtaacct tctacaaggt aacagttgtc ctccttccgt
     3421 atagcgttct tgccctgaaa gaaaccatac aggatcttgc ttttgttata gatgtcggaa
     3481 tctatcgagt tgaggtattt cggagccttc tcgctgatgg tcaatgacct gccgccaaag
     3541 ccgattattc ttccggaaag attaaatatt gggaacatca cccgattgac gaaaaaatcc
     3601 ctgtcatttt gggttacaag cccgatttgc ttcatcaaat caatattgaa tgcctttatt
     3661 tttgcttcat tgattagatt atgaccatcc ggaaaagcaa atcccaaacc gaatttttca
     3721 attgttacat cgtgaaaacc cctttccctg aagtaagata agccaatgtt tctgccatat
     3781 tcagtttccg taagattgga aatgtagtgt ttcatagcaa actcattgat gagcaagagg
     3841 ctttctcgct gtcttgccac ctcattttgt tccggcgtcc gtgcttcctc ttccacggtc
     3901 acattgtatt ttttggcgag atacctcaat gcatcaccat aggccatgtt ttcgtgttcc
     3961 atgatgaagc caaccggtgt gcccgagcga ccacagccaa agcatttgaa aatattcttt
     4021 ccgggagaaa ctacaaatga tggcgtcttc tcattgtgaa aggggcagtg cccaatcata
     4081 ttgactcccc gtcgctttaa taccagaaaa tcacctacca cctcttcgat gcgtgctttc
     4141 tggaatattt cttcaacggt cttgcttggt atcatacaat tctaaagaca ttcaactctg
     4201 tatagttcaa ctatcaaagg taaaacttta gaattggttg aaaaaaggat tctatggaat
     4261 attgtgcttt aattctcctt tctttcaaaa aataagtata aaagataggt tctagcggat
     4321 aaacatcatc tcccggtatt ttgccagtgg ccaaagtcca tcttccacta tttcttccaa
     4381 atgatctact gattccctga tctcagccat gattggctta accaaatggc agaacaattc
     4441 agcttcttct tcaggttcag cttcagagac ggctttatta accgactggt ttagtgtttc
     4501 gaccttttct gcaacattat taatatggtg agtaatttca ttcaataagt tactttgagg
     4561 cctgaaagcg tcctcctgta ggccaagttg ttttaaggta taaatgtttt cagctattcc
     4621 ggtctgatat ttataacatg ctggcaatac atatgacttg caaagatcag aaagcaccct
     4681 ggcttcgatt tccagcttta gattataatt ttcgaccatt acagtataat gagcctctag
     4741 ttctatttcg ctgaatatgc ctgtcttttg gaagagctca cttgttttgt ccatcttgta
     4801 cactttcaaa gcatccggcg tattgggcgt gttgctcaat ccccttttct ctgcctcttt
     4861 ttgccattct tcactataac catcgccttc aaacaatata ggaagggatt cattgatata
     4921 tttccttaaa atggacaaaa ttgcggtgtc catggattct cctttggatg taagtagatc
     4981 caactccttt ttaaattcag tcaactgatt tcctacaatt gtgttcatta tggtcatcgg
     5041 tgcagaacag ttcatggaag atcctaccat ccgtatttca aatttattac ctgtaaaggc
     5101 aaatggagat gttctgtttc ggtccgtatt gtcaagttca agattaggag atttcttaag
     5161 cagtgaaagc acctccttca ccattgtttt gtcactttct ttgccttccg caataaaatg
     5221 caacacttct gttagagctt ttccgacata aacggaaata atggcaggtg gagcttcatt
     5281 ggctcccaat ctgaaatcat tggatgcaga tgcaacactt gccctaagaa gatctgcatg
     5341 ctctttaact gctctgatcg tattcacaaa gaaagtcaga aacttaaggt cttttcccgg
     5401 atttttaccc ggagaaagga ggttcttccc ggtattggtc gctattgacc aattgttgtg
     5461 cttgccggat ccattgacac cattgaatgg tttttcatgc aacaataccg ctagtttatg
     5521 ccttttagcg acacgttcca tgatgtccat aagtaattga ttgtggtcaa tggcaatatt
     5581 gacctcctca tacattgggg ccaattcata ttgaccggga cctacttcat tgtggcgtgt
     5641 cctgaccgga atgcctaatt tgtgacattc tgtttccaga tccctcatat agttaaggac
     5701 tctttcggga atagatccaa aatagtggtc ttccaattgc tgccctttgt ggctttgccg
     5761 tcccagtaat gttcttcctg ttatcataag gtcaggtctg gcaataagga aagattcatc
     5821 aaccagaaaa tattcctgtt cccaacccag agtggcgtgg gttctttcaa tgctttcatc
     5881 aaatatcttg catacctcca aagcagcatt ctccagataa gctactgatt ttagcagggg
     5941 taatttgtaa tcaagggatt caccactgta ggtcacaaaa atagttggaa tacacagtgt
     6001 cttcccattc ccaacttcta taataaatgc cggtgatgat ggatcccatg aagtatagcc
     6061 tcttgcttca aaagtacttc tcagtccacc gccgggaaaa gaagaaccat ccggttcctg
     6121 ttgaaccaat tgttcacctc caaattcctc aatggctttt ccatctgctg taatggtaaa
     6181 gaatgaatcg tgtttctcgg cagtcttccc tgtcaaaggc tggaaccaat gggcataatg
     6241 cgtcgcacct ttgtccatgg cccaattctt catggcttca gctaccgaat tagctacttc
     6301 cttggtgagg ttagttcctg attgaattgc tgccttcaca ctaaaatagg ctgattcaga
     6361 aagatatttc ttcatcgctt catcatggaa tacattctcg ccataaaatc ctgagatttt
     6421 aaccttgtca ggcgtgttta caggaaaatc gatatcgaat ctattgtgta taatatcgat
     6481 tgcatttgac cttttaagac tcattttgtt tgaaaattga ttatttggaa aataacatta
     6541 ggaatgaccg caaagataat tacaaaaatt tttttctaac ctgttatata taataaattt
     6601 tataacttat cttgccgtta ttatggacaa gttttgaaat ggcttgcagg acttgatttt
     6661 ctgtattttc aacttaactt atccaggttg cattaattaa actttaacaa agttgaggta
     6721 tttaaaactt ctgattttta gtttgctcat acttttttgt cttcctttgg aggcacaagt
     6781 ggacggttca actgttgata ttgaggtgat agatgaagtg attgaatcaa tacttgcttc
     6841 caatccggac cttacggaag cccaaatcaa tgatcttacc gatcgcttgt ctgttcttgc
     6901 gcagagtaaa gtcgatctta atgaatccga tctgaaggta tttgaagaac tacttttgtt
     6961 aaatgaactt cagatacgtg agattttgtt gcatcgggaa gtgtatggtg actttattgc
     7021 attatatgaa ctgcagactg tcccttcttt gacactttcc gatatccgca gaatatggcc
     7081 ttttttgaag ggaataggaa caccgggcat atcgccgttt tcatggtcta tgctttacaa
     7141 gggaagtaaa accataatat cccgttattc cagagtgctt gagcaagctt caggatatgt
     7201 tggtgattct tctaaatatc ttggaagcag ggatcagata agttttagga tgcgtcatgt
     7261 tgttcctgga cgtgtgagca ttggaatcgg agttgaaaaa gatgccgggg agcccttgaa
     7321 tacaaattat aatccctttt tattcgattt tgttaccttt catgcctatc ttgagaatgt
     7381 ggcgaagcaa attaaacagc tggctttagg tgactttcag gtttctgccg ggcaaggatt
     7441 aatcatgttt tcagggtatg gttttggaag aaatgctttt actacagccg taaaaagaaa
     7501 cgaaagggcg atcagacctt ctacttcatt gaatgaaatt aatgctctgc gtggcgctgc
     7561 tgtgggaatt gagttggccc ccggatggca gatgatagcc tttgcaagcc ttaaaaaaag
     7621 tgatggtact ttaataattc ctgattcatt atccggtgat tctgatgtgc ttatttcttc
     7681 tttgcagatg tcgggattgc atcgtactga aagtgaaatt aggaatggca aacatatcag
     7741 tatgtttacg accgggggat ctttgaaata taacggtaac cgatttcaat ttggagcaaa
     7801 tgccgtttat gatcaacttg ataaaagagt tgagccagcc gatcgagttt ataaccggtt
     7861 ttattttaaa ggaaaaaatg ccctgaatgc cagtgtcgat tatacatatt atttccgcaa
     7921 cctgatcttc tatggtgaga cagcaattaa ccctgagaat gcagtagcaa ctgtcaatgg
     7981 cttattagtg ggtttgcacc cgaaggtcaa tttagcctta ttgtttcgac atttgccaaa
     8041 ggaatattac agtcttcatg gaaaagtatt ttccgaccaa agccagacaa ccaatgaaac
     8101 aggattttat gctggactgg aagtaaagcc aagtccgcga ttcttagtca acatatatgc
     8161 tgacttcttt cgacatggat ggcctacctt tagtgcaaat ggcccctctg aaggaagcgc
     8221 ttatctattt aaggcatctt atcaaaaaag aaagttctgg gatggttatc tgcagataag
     8281 atatcaaaat gagcaagtga gtgggggagg agatgctctg attcctcctt tatatttcag
     8341 acaaaagaca gacttaagac tgcattttaa taaaagggtc agtaatttga tgacctggtc
     8401 aacaaggtta gactataact atatgaaatt gccgggagac aggaaggaga atggatatct
     8461 gatgtatcag gaattttggt ttaaaccgct aggaaagcca tataatggtt ttttaaggtt
     8521 ttcacatttt aatattgact cctatcaaag tcgtatatat tcctatgagc actatcaagc
     8581 ttatgattca cgaaatgtgg cattcgaagg cgttggcaat agaatggtga caggaattag
     8641 atggaaattc aataatggaa tagtgcttga aggttcatat aatataacta aatatacgga
     8701 tcgcaatcaa atcggttcag gaaacgatag aatcctagga ccggtgaaat ctgaaataag
     8761 ggcgcagatt aggtatggat tcaacgattg aaaataggga aacatccctg aattacattt
     8821 tgctataatt ctggacaaaa acattccagg gggttttttt gtagtggtct tcgccaaaaa
     8881 atcgcataat cttataaaaa tccttgggtt cctgatagcc ttgtatgggt tggatgacat
     8941 tcagttcttc atcaataaac actacagtcg gatagcttag tcttccacct gtcaacagta
     9001 aagcaaattc attgtaaccc ctgcctttac tcccgacata actatatacc ttattgttaa
     9061 attcaatatt gtctttggtt tcggcattaa atttgacggg ataataattt ttgttgatat
     9121 agttgatgat atcatcgtta gcaaaggtgt ttttgtccat taccttacac caaccacacc
     9181 agtcggtgta tagatcaaca ataatcttcc tgggctttat tttattttgc tggaccgctt
     9241 catcgattgt gtaccatttg attcttgcat ttgtttttgt ttcggttcgc gatgtagctt
     9301 gccccgtcag tttccctgcg aaacctaaca aaatgcctat gaccatgccg aaaatcaaac
     9361 tatttttcaa ggtttaatca attttctgtt acaaataaac gacattagaa catactatgc
     9421 atcattttag taaaaatcta cctgagtttt aataatactt taaccattgc cagttcaaaa
     9481 atgtcattct gtgatataaa ttatttgtga aaacagattg ttggaagaag tgtatattga
     9541 attaggtaga tgtgtaatac ctgatagtca ggctaatccg gcaccttcag tccaaaagat
     9601 atctgaatga gtcaatttta gcatatgtaa aatagttgtt ttttcatgta atgtaattag
     9661 cgtttgatta attccgtatt aatcatctac ctatttaggt tagtatgtta tcacaacact
     9721 tccagatttt tagcaaactt gattcattgt tgttatccat cttagtaata aactggctat
     9781 tgcctgtcct aaagaatgaa ttaggtagat atctaatacc tgactatgag gctagtgtgg
     9841 tctctccggg ctataaagta tttgaattta tcaatttcgg cacaagtatg attctgatgt
     9901 tttttaaaat gtaagaagcg attgaatgat tgggtattaa ccatctacct aattcggatt
     9961 gccaccttca cccaccacat cccttccatt tcggttcctc actcaaccac tgacaacaga
    10021 attgaaacgc cctcttccag acaccatcac aattttttta ccaattatca cagtttattt
    10081 taacttagca tttacaatgc tgaaatttca aactcttaag gtggatattg cggatctttc
    10141 aacaaatcag gcattgtgtt gacacgctat ttatccgtga aattggttgg ttcatgaagt
    10201 ttgtaaaaca gttctttatt atattttttc tggtgatttc agtcgtgctg atgatcggaa
    10261 tatttatgga taagaactat catgtcgaga ggacaataac aatcaatact ccggcttatg
    10321 aggtatttga ttatatcagg tatctcaaga accttgacgc ttattctttt tggtcaaaaa
    10381 ttgatccaga aatgaagcgt cgatatgaag gc
//
LOCUS       DAOQQN010000061        10360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2348_length_10360_cov_5.437263, whole genome shotgun sequence.
ACCESSION   DAOQQN010000061 DAOQQN010000000
VERSION     DAOQQN010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10360
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2348_length_10360_cov_5.437263"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="PLG24_03660"
     CDS             complement(<1..1116)
                     /locus_tag="PLG24_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HMZ24013.1"
                     /translation="MSFHLESAYKPTGDQPEAIRQLVDGVNGGDRAQVLLGVTGSGKT
                     FTMANVIKELNRPTLILTHNKTLTAQLYGEFREFFPDNAVEYFVSYYDYYQPEAYIAV
                     TDTFIEKDLQINEEVDKLRLRATSSLLSGRNDVIIVASVSCIYGMGNPDDYKNSIIRL
                     QRGQIISRNAFLYKLVDSLYARTEAMLTRGHFRVKGDTVDIALPYVEHGLRIHFYGDE
                     IEEIEILEVESAKAIQKIETAAIYPANLYVAPKDKLNDILHSINHELKEHESYLESEG
                     KYLEAKRIHERTTFDIEMIRELGYCSGIENYSRFFDGREPGTRPFCLLDYFPDDFLMI
                     IDESHVTIPQLRGMWGGDRSRKTNLVNFGFRLPSAMDN"
     gene            complement(1123..1755)
                     /locus_tag="PLG24_03665"
     CDS             complement(1123..1755)
                     /locus_tag="PLG24_03665"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HMZ24014.1"
                     /translation="MKNVSLIFNVILTIAVAFLFYLNFKKPVQVTTITTSSGKDSTIK
                     VDIPETLPEGVPMGKILYVDIDTINEKYTFIKNQKAIIERQGAQAEASLKAKAQSLQS
                     EFIEYQKKAQAGELTQQQAMAMEKSLTDKQQALAQQEQKLSTQLVEKTQKIQEELNAK
                     MRKELKVFMEKYGADYILGYTEGANILLPNPKLNITKEVLNRLNESDKKK"
     gene            complement(2050..3174)
                     /locus_tag="PLG24_03670"
     CDS             complement(2050..3174)
                     /locus_tag="PLG24_03670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ24015.1"
                     /translation="MSIEILEVKNKSDLKSFIYLPEKIHRNHANWVHPIYLDEKQFFN
                     FDKNEAFRHNRTALFLARENGRVVGRIMAIIPDEYNNMKNVRTARFCYFEAPENKRIF
                     DALLNKVEQWAKDAGCNLMVGPMGFSDKEPQGFLTAGYNEKTMIVTNCSFRYMKEFLE
                     ARDYSPFVELCQYDVPLSNQIIQRYRALADRVESNMRVTVHEFTKTSEIKPFVQSVFR
                     LINTTYTDIYGFTKVTEKEAEDFANRFIPLLNPRLIKLITDESGEVVAFVIGMPDLSG
                     AIKKSRGRIFPFGWFHILRAFRNSKRMVLLLGAVKNEMQNKGLDAVLAVRLIRSALDL
                     GFDIMDSHLIMRKNIKMRREIERLENHRMYKEYTIYSKEL"
     gene            complement(3211..4095)
                     /locus_tag="PLG24_03675"
     CDS             complement(3211..4095)
                     /locus_tag="PLG24_03675"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMZ24016.1"
                     /translation="MSKTLRNIPLFWFSMLGYFAFVEIIVLWRDPQLFLSGQIMMNRL
                     IYIIIFGLLLILQRKLPVKQFLFFSILAAYAALTLLYKETALLNRLFYPVLDPYLSEL
                     DFQLTGIQPALVFSERFDSVFFSELMFLGYFSYYLTPLAILILIFFKKFELMEQFGFV
                     LITSFLLYYLVFIFIPAIGPQFYFSYPDNFVEAKGLFGHLVKLIQENGEAPTAAFPSS
                     HVGVALICMIWLWRNAKKYVYYFLPNVFLLILATVYIKAHYLVDVLAGAITAPLVFFL
                     VVKLYDQLKYRSTGSLPQ"
     gene            complement(4733..5968)
                     /locus_tag="PLG24_03680"
     CDS             complement(4733..5968)
                     /locus_tag="PLG24_03680"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ24017.1"
                     /translation="MKILVAGGGASGFMAAITAAERWPHHTVIIAEKSHRVLSKVLIS
                     GGGRCNVTHRPLDIHDFSDQYPRGKRFLKKVLHEFGAADAIQWFESRGVRLKTEVDGR
                     VFPASDDSASIAGCLEGEARRLGIQVLLKKGLDSFEKVDSGFQVHFSDGTKEVFDRII
                     LATGGFPKSSSYDFLREAGIAIKEPLPSLFTFNSPGNPISKLMGVSVPFAQVKIGGTK
                     LVHHGPLLITHWGFSGPAVLKLSAFGAELLAEMKYEFNVEIRWCNDITREECRAMWLG
                     WRDKKVKTLIANHSGLNLPERLWNFLVEEAQISDKTRWADIGNKGIDSLLNQVFSNIH
                     AIKGKTTFKEEFVTCGGVCLDKVDHRTMEYKNIPGLYFAGELLDVDGVTGGFNFQNAW
                     TTGFIAGRSVGQNYSSVRL"
     gene            complement(5975..7867)
                     /locus_tag="PLG24_03685"
     CDS             complement(5975..7867)
                     /locus_tag="PLG24_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HMZ24018.1"
                     /translation="MSDQIKHECGIALIRLRKPLEFYREKYGTALYGINKLSLLLQKQ
                     RNRGQDGAGIATIKLNPEPGTRYISRKRSNSANYLNDLLKSVYKYFNNLPEERLNDPA
                     WLKANRPYMGEVLMGHLRYGTHGLNSIETCHPFMRQNNWMSRTLVLAGNFNMTNTDEL
                     FQELIELGQFPKEKSDTVTMLEKIGHFLDNEVQNLFDHFKRIGYSNQEITEQMKSSLD
                     VNNVIRKAFRKVDGGYAIMGMIGTGEAFVVRDPHGIRPAYYYMDEDVIAVASERPALQ
                     TAFGVRYKNVHEVPPGHTMVIRTNGEVEFQPFTDPAPRKSCSFENIYFSRGNDRKIYL
                     ERKRLGEQLAKPIMEMVNYDFDNTVFAFIPNTAETAFYGLVEGLEKELNEVKKKKILD
                     LGASADATSLDQILSTRVRTEKLVVKDAKIRTFISDDSSRGDLMSHVYDVTYGIVKND
                     RDTLVLLDDSIVRGTTLKQSIINILSKLFPKKIIIVSSAPQIRYPDCYGIDMSVMKSF
                     VAFQAVIEMLEESGREELIEEVYHKCKEQEHLPKEMMTNHVKSLYDVFTDKEISERIA
                     RIVRPDNLPYDLEILFQSIDGLHAACSEEVGDWYFSGDYPTPGGTKVVNQAFINYMEK
                     STERAY"
     gene            complement(8472..>10360)
                     /locus_tag="PLG24_03690"
     CDS             complement(8472..>10360)
                     /locus_tag="PLG24_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ24019.1"
                     /translation="VFLIYVVTIFNVINAQELEYLLPEATISSAFNKPPVNASFLIRH
                     KADSNYQYTLKSTSGMLREDGIYLKAYNEGGLNTAGSRGYGSSHTKVFWNGFLLNSPL
                     NGTIDLGLQALDPLATDILFNKSNSPVEGSISGAGGSIWISDRIPKGDNAVRAALSAG
                     SFSTYKSALSYTAHRGRFSNHLLLSLMTSANDYRYPSVTDFRRPLVRLNNADVLLYGI
                     NNASSLILPSSRVDLRFNYTRAARGIPPPITSTGPIAFQQDEGIKLGMAWSTALNSRS
                     DLSFNAAYMRDVLFYYPVRNADSKTEYYVNAAPLSARWNYNRWSHHLNIHLSSTFYHV
                     NSTELTAPDEVQTYGTIELDRDKAGLLQYGFSVQQLMVKTRLIEPSYRLYVGTRIVKG
                     LNWNLSFASTKQVPTINDRYWPLSGNPDLKVETNRSLELGLNYSQEFGNLQFVKGSLA
                     IFNGMVDDWIGWIQVSGDFWKPFNVQKVQSRGLDLRGDYHWAVSSRVKLLASYNLSIS
                     NPIVIKKYSGSPAQEGKRLIYTPAFQHAACISLELDNHLMKVMPRYTSSRFTTADNKV
                     EYSLDPFVLLDFEYNYHFKIRKSKFTAVFKVNNVFNEYYEMIAYRPMAGRYFEVGLGA
                     GFGQ"
BASE COUNT         3020 a         2400 c         1965 g         2975 t
ORIGIN      
        1 gttatccatg gcactgggta acctgaatcc aaaatttacc agattggttt tccgactgcg
       61 gtcacctccc cacataccgc gtaactgtgg gatggtaaca tggctttcat ctatgatcat
      121 caggaaatca tccggaaaat aatcgaggag gcaaaacggt cttgtaccgg gttccctgcc
      181 atcaaaaaac ctggaataat tctcaatacc gctgcaatag cctaactccc tgatcatctc
      241 aatgtcgaag gtggtgcgtt cgtggatgcg cttggcttcg aggtattttc cttcggattc
      301 cagataggat tcgtgttcct tcaattcgtg attgatggaa tgaaggatgt cattgagttt
      361 atccttaggc gccacataaa gattggccgg ataaatggca gctgtttcaa tcttttgtat
      421 cgcctttgcg gattccacct ccaggatttc aatctcttca atttcatcgc cataaaaatg
      481 aattctcaga ccatgttcca catatggcaa agcaatatcg acagtatctc ccttaacccg
      541 gaaatgtccg cgggtcaaca tggcttcggt tcgggcataa aggctatcca ccagcttata
      601 cagaaaggca tttcttgaaa tgatctgccc acgttgaagg cggatgatac tattcttata
      661 atcatccggg ttgcccatgc catagataca agatacggat gccacaatga tcacgtcatt
      721 tcggcctgat aacagactgg aagtagctct gagtctcaac ttatccacct cctcattgat
      781 ctggaggtcc ttttctataa aggtgtccgt gacagcgata taggcttcgg gctggtaata
      841 gtcataatag gagacgaaat actccacggc attatccgga aaaaactccc tgaactcccc
      901 atacaactgg gctgtcaatg tcttattgtg ggtgagtatc agagtaggcc tgttgagctc
      961 cttaatgaca ttggccatgg tgaatgtctt gccagagccg gttacaccca gcagcacctg
     1021 agcacgatct ccgccattta caccatccac aagctggcgt atcgcttccg gctgatctcc
     1081 ggtaggctta taggcagatt ccaggtgaaa agacatttaa ttctatttct ttttatcaga
     1141 ttcgttcagt ctatttaaaa cctctttcgt gatatttagt ttcggattag gaagcaatat
     1201 attagcgcct tcggtataac caagtatata gtccgcgcca tatttttcca taaatacctt
     1261 gagttccttc ctcatctttg cattcagctc ttcctgaatc ttttgcgttt tttctacaag
     1321 ctgggtagaa agcttttgct cctgttgggc caacgcctgt tgtttgtcag tcaatgactt
     1381 ttccattgcc attgcctgtt gttgtgtcaa ttcacctgcc tgcgcctttt tctgatactc
     1441 gataaactcc gactggagag actgcgcctt ggcttttaga cttgcctcag cttgtgcgcc
     1501 ctgcctttcg atgattgctt tttgattttt gataaaagta tacttttcat tgatggtatc
     1561 aatatcgaca taaagaatct tacccatagg gacaccttcc ggcaacgtct cagggatatc
     1621 caccttaatg gtagaatcct tcccggagga tgttgtaata gttgttacct gtaccggttt
     1681 cttaaaattc agataaaaaa gaaaagcaac ggcaatcgtc aggataacat tgaaaatcaa
     1741 ggatacattt ttcataaaaa ttattgttga tattatgctt taaatcaatc acatcgggct
     1801 ccaacactga ttttaccatt cgctttcctg attcagccat agttccagga ccaaactaca
     1861 aaccatgcgg agctttgcaa acgaaacgct attttaccca atttgccagg ttgttttgtc
     1921 tgcaaaatta aacggcaaaa gtaatcaaaa aattgaccgt acagggagtt ataatggtgc
     1981 attttaaaaa tgagaactga ttctattcat cttcagtctc ttttgtcaat aatcagcccg
     2041 ggataccgat cagagttctt tcgaatatat ggtgtattct ttgtacatcc ggtgattctc
     2101 caggcgctct atttcccgtc tcatcttgat attcttcctc atgatgaggt ggctgtccat
     2161 aatatcaaaa ccaagatcca gggcggaacg aatgaggcgc accgccagga ctgcatccaa
     2221 ccctttgttc tgcatttcgt tcttaacggc tcccaacagc aaaaccattc ttttagaatt
     2281 cctgaatgcc cttaaaatat gaaaccaccc gaaagggaaa attcttcctc tgctcttctt
     2341 gattgctccg ctgagatcag gcatgcctat aacgaatgca accacttccc cgctttcgtc
     2401 agttatcaac tttattagtc ttggattcaa aaggggaata aaacggttgg caaagtcttc
     2461 tgcttctttt tcagtgacct tggtaaatcc ataaatatca gtatatgtcg tgttgataag
     2521 ccggaaaaca ctttgcacaa atggctttat ttcagatgtt ttggtaaatt catggacagt
     2581 cacccgcata ttactttcta ccctgtcggc cagcgcacga tatctttgaa tgatttgatt
     2641 agaaaggggg acatcatact gacacaattc cacgaaagga ctgtaatccc tggcttccag
     2701 aaactccttc atgtacctga agctacagtt ggtcacaatc atcgtcttct cattataacc
     2761 cgcagtaagg aagccctgtg gttccttgtc cgaaaaaccc atagggccta ccatcaggtt
     2821 acaaccggca tccttcgccc attgttccac cttgttcaaa agagcatcaa atattcgctt
     2881 gttttccgga gcttcaaaat aacagaacct tgctgtcctg acatttttca tattattgta
     2941 ttcatccgga atgatggcca taattcgtcc cacgaccctt ccattttccc tggccaaaaa
     3001 caaggctgtc ctgttgtgcc gaaaagcttc atttttgtca aaattgaaaa actgcttttc
     3061 gtccaggtag attggatgga cccaattagc gtggtttcgg tgtatttttt ccggtaggta
     3121 aataaaggat tttaaatcac tcttattttt tacttccaat atttcgatac tcataagaaa
     3181 aggctaaaga atcaacaatc atattcagaa tcattgaggc aagctaccgg tcgatctata
     3241 tttcaattga tcatatagct taacaactaa aaaaaatacc aaaggtgcag tgattgcacc
     3301 ggcgagtaca tccaccagat aatgcgcctt aatgtaaaca gtagctaaaa tcaacaaaaa
     3361 aacattagga aggaaataat agacatattt ttttgcattt ctccacagcc agatcataca
     3421 aatcaatgct actcccacat gtgagcttgg gaaggcagct gtcggcgcct caccgttctc
     3481 ttgaatcagt ttgaccagat gcccgaataa accctttgct tcaacgaaat tatcaggata
     3541 agaaaaataa aactgcggac cgatggccgg aataaaaata aacaccagat aatacaataa
     3601 gaaggaagta atcagaacaa aaccgaactg ttccatcaac tcgaattttt tgaagaaaat
     3661 caaaatcagg atggccaacg gagtcaaata ataggagaag tagcccaaaa acatcaactc
     3721 actgaaaaag accgaatcga atcgctctga aaataccagg gctggttgaa ttcccgtcaa
     3781 ctgaaaatca agttcagaca gatagggatc cagcacagga tagaagaggc gatttagcag
     3841 ggcagtttcc ttataaagca atgtaagcgc ggcataagct gccaggatcg aaaaaaagag
     3901 aaattgcttt acgggcaatt tccgctgcag gataagcagc aatccaaaga taatgatgta
     3961 aatcagtctg ttcatcatga tttgcccgga taggaacaac tgaggatccc tccataacac
     4021 aataatctcc acaaaggcaa agtaacctaa catgctgaac caaaaaagag ggatatttct
     4081 tagggttttg gacaaaacta cttaaacttt attttagtca aagaaacaaa tttctttaaa
     4141 aatgtgaaag aacgattgac cgcgtgattc atacaacttt tatcaatccg aatctcaaag
     4201 aatcatttac gccgattcaa aacaacaata caccttcatt caattaatta attattgatt
     4261 atctgttatt tatacattcg ttttttatat ctatacaatt tccaaatcat caacccagag
     4321 caagtctgtt atcactcctt tttcaatgaa aaaccccact ctatttagta acctatttta
     4381 cctggtcgtt ttccaatttt tccttgcgaa agcacctgta tttaaacgct tccaaatcat
     4441 cagtccgcta ttccttcaaa cgaatattaa cataaacacc aatcaatctt ttcatacttt
     4501 gctattttcg gaaaatgcca tagccatctg ttaccacagt gacaaaataa attagcccct
     4561 cacgtttttg ggccatcaat caaaatatgc tttaacgagt taaaagaaag gttcctatca
     4621 cataatgtac cggtaaatac tgacggcgca aaattctcaa aataactact gctaaccttt
     4681 attcacaggt gaaatttgtt ccatacacca ttgaagctaa gctcacaata tgttataacc
     4741 tgacagagga atagttctgc ccaacacttc taccggcaat gaaacctgtc gtccaggcat
     4801 tttgaaagtt aaacccgccg gttactccgt ccacatccaa taattcacct gcaaaatata
     4861 aacctggtat gttcttgtat tccatggtgc gatgatcgac cttgtccagg caaacaccgc
     4921 cacaagtgac aaattcctcc ttaaaagtag tctttccctt gatagcatga atgtttgaga
     4981 aaacttgatt gagaagggaa tcaattcctt tattaccgat atctgcccat cgtgtcttgt
     5041 ctgaaatctg agcttcttcc accaggaagt tccacagtcg ttccggaagg ttcaatcccg
     5101 aatgatttgc gatcaatgtc tttaccttct tatcccgcca tcctaaccac atggcccggc
     5161 actcttcccg ggtaatgtca ttgcaccacc ggatttcaac attgaattca tacttcattt
     5221 cggcaagcaa ttctgcacca aaggctgata atttcagcac ggcaggccca ctgaatcccc
     5281 aatgtgttat aaggagcgga ccgtgatgaa ccagttttgt tcctccaatc tttacctgtg
     5341 caaacggcac agatacgccc atcaatttgg agatcggatt acccggacta ttgaaggtaa
     5401 agaggcttgg tagaggttcc ttgattgcaa tcccggcctc ccggagaaaa tcgtaggagg
     5461 acgatttagg aaagcctcct gtagcgagga taatacgatc aaaaacttct tttgtaccat
     5521 ccgaaaaatg aacctgaaaa ccggaatcga ccttctcaaa agaatccaat ccttttttga
     5581 gtaatacctg tattcccagc cttcttgctt ctccttccag gcagccggca attgaggctg
     5641 aatcatcaga agccgggaaa actctcccat cgacctccgt cttcagccgg acgccccgtg
     5701 attcaaacca ttgaatagca tcagccgcac caaattcatg gagaaccttc tttaaaaacc
     5761 tttttcctct tggatactga tccgaaaaat catgaatgtc caacggtcga tgagttacat
     5821 tgcatcgacc accacctgaa atcaatactt tggagagtac acgatgtgat ttttcggcaa
     5881 tgatgaccgt atgatggggc catcgctctg ccgcagtaat agccgccata aaaccggagg
     5941 ctcctccacc tgcaacaaga atcttcatca gaaattaata agcccgctcg gtactctttt
     6001 ccatataatt gataaaggcc tgattgacca ccttagtacc gccgggtgtt ggatagtctc
     6061 ccgaaaaata ccagtcccct acttcctctg agcatgcagc atgtaagcca tcgatggact
     6121 gaaacaggat ttccaaatca tatggaaggt tgtcaggtct tactattctt gcaattcttt
     6181 cactgatctc cttgtccgta aagacatcat ataatgattt gacatgattg gtcatcattt
     6241 ctttcggcag atgttcctgc tccttgcact tgtgatacac ttcctcgata agttcttctc
     6301 gcccgctttc ttccaacatc tctattaccg cctggaacgc cacaaatgac ttcatcacgc
     6361 tcatgtcgat accataacag tccggatacc tgatttgagg cgccgaggaa acaattatta
     6421 tttttttcgg gaataatttg gacaggatat tgatgatgga ttgtttaagt gttgttcccc
     6481 gtacgatgga gtcatccagt aaaacaagcg tatcccgatc attcttaaca atcccatatg
     6541 tcacatcata gacatgagac atcaggtcgc ccctgctcga atcgtcagag ataaaggttc
     6601 tgatttttgc atctttgacc accaacttct cggttcgcac ccttgtagac aggatttgat
     6661 ccagtgatgt cgcatctgcg cttgccccca gatcaagaat ttttttcttt ttgacctcat
     6721 tcagctcttt ttccaggcct tcgacaagtc cgtagaaagc tgtctcggca gtatttggaa
     6781 tgaaagcaaa aacggtatta tcaaagtcat aattaaccat ttccatgatt ggtttggcca
     6841 actgttcacc caggcgcttt ctctcaaggt agattttacg gtcgtttcct cttgaaaaat
     6901 aaatattctc aaaagaacag gattttcgtg gagccggatc agtaaatggc tggaattcca
     6961 cctctccgtt tgtcctgatg accatggtat ggcctggtgg cacttcgtga acattcttat
     7021 aacggacacc aaaggcagtc tgcaaggcag gtcgttcgct tgctacggca atgacatctt
     7081 catccatata ataataagcc ggacgaatac cgtgagggtc tctaaccaca aaggcctctc
     7141 cggtgccgat catacccata atagcataac cgccatccac ctttctgaag gctttacgga
     7201 taacattatt gacatccagt gaactcttca tttgctcggt gatttcctga ttggaataac
     7261 caatgcgctt aaaatgatca aaaagattct gtacctcatt atccaggaaa tgcccaatct
     7321 tttcaagcat tgtaaccgtg tcggactttt ccttgggaaa ctggcccaac tcgatcaact
     7381 cctgaaataa ttcgtccgta ttggtcatat tgaaatttcc ggccagtaca agcgtacggc
     7441 tcatccagtt attttgccgc ataaatggat ggcaagtttc aatggaattc aggccgtggg
     7501 ttccatagcg taagtgcccc atgaggacct cacccatata aggcctattg gctttcagcc
     7561 aggccggatc gttaagacgc tcttccggca ggttattgaa atacttataa acacttttga
     7621 gcagatcatt caggtaattg gcgctgttgc tccttttgcg tgaaatatac ctggtgcccg
     7681 gttcaggatt gagttttatg gttgctattc ctgcaccgtc ctggcctctg tttctttgtt
     7741 tttgcagcaa caggctcaac ttattgatgc cgtaaagtgc tgtcccatac ttttcccggt
     7801 aaaattccaa tggctttcgg aggcggataa gtgcaatccc acactcatgc ttgatttggt
     7861 cgctcatgta agaaggttct ccttttaaaa gtaaagcaca aagttacgct tataatatca
     7921 tattgaaata aaaaaatgta atgatatttg attaataagc aattacagga caaataaaga
     7981 gcaacatagg atggacagtc ttcagtctgg ttactgacct ttttgaaaag gttcattcca
     8041 acaatagcga ttaaccgggc ataccatggc tgcaaagctc atccagtgta gtattcggta
     8101 gcacaactta catctacaaa aagtatatag attgtcgctt tttgatctct tcaattggta
     8161 cgataacata attggcacaa tgagggactg aaatggggat aatgaaaagt gggaatgcag
     8221 ccatgagtaa ataataatca ataaactaat catattcata ttaatatttt taatgtatat
     8281 taatatgatt atctgattat tatagtcatt tcaatatttt tccggccaaa ctggcatcat
     8341 ggatctatca aggcatctgc aattatccaa tttttttcat cctcttcctg aaatgtaagg
     8401 aagtacaaaa acggataaaa aaacattgaa taaatacatt cttctatcct aataaaatgc
     8461 cttcaaatta gttactgccc aaaacctgca cccagaccaa cttcaaaata tctccctgcc
     8521 atgggacgat aggcgatcat ctcgtaatat tcattaaaaa cattattaac tttgaatact
     8581 gccgtaaatt ttgactttct aatcttaaag tgataattgt attcaaaatc caacaaaaca
     8641 aagggatcca gactgtattc caccttatta tctgcagttg taaatcttga cgacgtatat
     8701 cgcggcatca ctttcatcag gtgattgtcc aactccaggg aaatgcaggc cgcatgttga
     8761 aaggcaggtg tatagatcag ccgcttccct tcctgagcag gactgccgga atatttctta
     8821 ataacaattg gattggaaat actcaggtta taggacgcca aaagcttcac ccgggaagag
     8881 acagcccaat gataatcacc tcgaaggtcc aaccccctgg actgcacctt ctggacattg
     8941 aaaggcttcc agaagtcacc actcacctgt atccagccaa tccaatcatc aaccatgcca
     9001 ttaaaaattg ccagggagcc ttttacaaac tgcagattgc caaattcttg tgaataattc
     9061 aaacccagct ctaaggatcg attggtttca actttcaggt caggattgcc cgaaaggggc
     9121 cagtatctgt cattgatggt aggcacctgt ttggtagagg caaatgacaa attccaattc
     9181 aatcccttca cgattcttgt gcccacatac aaccggtaag acggttcgat cagtcgtgtc
     9241 tttaccatca attgctgcac gctgaacccg tattgaagca gaccggcctt gtctctgtcc
     9301 aattcaatgg ttccataggt ttgcacttca tcgggagcag ttagttcagt gctgttgaca
     9361 tgataaaaag tgctgctcag gtgaatattc agatgatgac tccatcgatt gtaattccac
     9421 ctggctgaca gtggtgcagc gtttacataa tattctgttt tagaatccgc attcctaacc
     9481 gggtaataaa acagaacatc cctcatatag gcagcattga aactaagatc tgatcgactg
     9541 ttcaaagctg tactccacgc catacccaac ttaatgccct catcctgctg gaaagcaatt
     9601 ggccctgtac ttgtaatcgg cggaggaatc cccctggctg cccgtgtata gttaaacctg
     9661 agatccaccc ttgaagaagg taagatcaag ctggacgcat tattgattcc ataaagcaag
     9721 acatcggcat tgttcagcct gactaatgga cgtctaaaat ccgtcactga aggataacgg
     9781 tagtcattag ccgatgtcat caacgatagc aaaagatgat tactaaatct tccccggtgt
     9841 gcagtataag acaaggcaga tttgtaggta gaaaaagaac ctgcggacag ggctgcacga
     9901 accgcattat caccttttgg aattcgatcc gagatccata tacttcctcc ggcaccggaa
     9961 atgctccctt ctaccggact gtttgattta ttgaataaaa tatcggtagc caaaggatca
    10021 agtgcctgta atccaaggtc aatcgtacca ttcagcgggc tattcagtaa aaagccattc
    10081 caaaaaactt tcgtatggct actcccgtaa cctcggcttc cggcagtatt tagtccacct
    10141 tcattatatg ctttaagata aatgccatcc tcccgcagca tccccgaagt tgacttcagg
    10201 gtgtattgat agttgctatc cgccttatgt ctgattaaaa aggaggcgtt gaccggtggt
    10261 ttattgaaag cagaagaaat agtcgcttca gggagcaggt attccaattc ctgagcattg
    10321 ataacattaa aaatggttac gacatagatg agaaatactg
//
LOCUS       DAOQQN010000062        10156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2418_length_10156_cov_5.748045, whole genome shotgun sequence.
ACCESSION   DAOQQN010000062 DAOQQN010000000
VERSION     DAOQQN010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10156
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2418_length_10156_cov_5.748045"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="PLG24_03695"
     CDS             complement(<1..1129)
                     /locus_tag="PLG24_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HMZ24020.1"
                     /translation="MTKSVEAVNNRHNSEPHSKLAAAFNKDAARVGWHDETLWWVRAK
                     RDKMAYQLPEWEALRQAASEIKSHTLANLGEYLVEFEQKAVENGLVVHWAVDADEHNR
                     IVHGLLKGNGIDRIVKSKSMLTEECGLNEYLLKQGIDVIDTDLGERIVQLAGEAPSHI
                     VLPCIHKKKEEIGDLFAEHLDTPKGCSDPKILTEAARQHLREAFLSRRAAITGVNFAI
                     AQTGEFVVCTNEGNADMGAHLADLHIACMGIEKIIPRRKDLGIFLRLLARSATGQPIT
                     TYSSHFRKPDRERKIHIVIVDNGRSKQLAREQFRNSLKCIRCGACMNTCPVYRRSGGH
                     SYHTAVAGPIGSILAPNLDMRQYADLPFASTLCGSCTHVCPV"
     gene            complement(1113..1841)
                     /locus_tag="PLG24_03700"
     CDS             complement(1113..1841)
                     /locus_tag="PLG24_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HMZ24021.1"
                     /translation="MKVALFVPCYIDQFFPQVAKATLHLLEKLGCTVEYPSNQTCCGQ
                     PMANAGYEYLAEGCADIFRQCFDRYEYIVSPSGSCVLHVKDHVMPRSNESDGPAVMEL
                     SEFLTDVMEITSLDASCPYRVGLHNGCHAIRGLGLSSMSEVPGNRFSKPGSLLRMVNG
                     LELVALDREDECCGFGGTFSVFEETVSVRMGNDRIDDHIRHGAQYITGTDMSCLMHLE
                     GLLRRRKCPVRVVHLAEILNDEIR"
     gene            2172..3254
                     /locus_tag="PLG24_03705"
     CDS             2172..3254
                     /locus_tag="PLG24_03705"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HMZ24022.1"
                     /translation="MKNPFFKYLAFLSIMMIPLLFARCKEDANDTNAGKKELLRGIDI
                     SFLPQIRQYGISFKNQSGQEGDLLKIVKENGVNLVRIRLWTGNPGKHSGEDEVYALAE
                     ECRNQGFKILLDLHYSDTWADPGHQTIPASWIGLDINQLKDSVYRYTFRVTSKCRPDY
                     IQIGNEINAGLLWPTGHISRPASMDELLKVGCNAARDAHPAIKILLHYAGIDGSIDFF
                     KKRNETGIDYDIAALSYYPYWHGSTIADMGNTLNDIRFYTKKEVMIVEFAYPFTLKWS
                     DHTNNVIGLESQLSPGYPATAEGQAVFVRTIRTVCEKYKAKGLCYWGGEWVAFKGDTS
                     SNGSSWENQALFDFDFRANPALTEYR"
     gene            3325..4119
                     /locus_tag="PLG24_03710"
     CDS             3325..4119
                     /locus_tag="PLG24_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ24023.1"
                     /translation="MELNDKWVWVTGASSGIGKAIAIELSFHKANLVLMSRNVSELKK
                     TADACNPNVNIIIQPIDLADNASIDEAVRVLLHDKKIPPHILINNGGISQRATALDTV
                     DAVTRKIFDINFFGTVYLTRALLPRMIEMGGARIAVITSLVGIIGSPKRTSYSASKHA
                     LHGYFDSLRAELYKEQIGVTLICPGFIRTNISLSAVTGDGTAQNTMDEKTGNGMDPAK
                     LAKIAVRGIMREKEEIYIGGKEILAIYLKRFVPTIFSKILRKTKVT"
     gene            4663..6189
                     /locus_tag="PLG24_03715"
     CDS             4663..6189
                     /locus_tag="PLG24_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMZ24024.1"
                     /translation="MDISFLKTLGINEINPGTTTGTIWFEPEEYLDSYSPVNGKLIGK
                     AGITTEAQYEDIINKSTEAFKFWRNIPAPKRGEIVRQYGDVLRANKDALGRLVSYEMG
                     KSLQEGWGEVQEMIDICDFAVGLSRQLYGLTMHSERPGHRMYEQWHPLGIVGIISAFN
                     FPVAVWSWNSMIALICGDVCVWKPSEKVPLSAIACHKLLEEVLRKNNIPEGVTCLING
                     DYRIGELMTKDPRVALVSATGSVRMGKIVGATVAARLGKSLLELGGNNAIIVTPDADL
                     NIVLTGALFGAVGTAGQRCTTTRRLIIHESHYENVKQKLVKAYGQLVIGNPLDAGVHV
                     GPLIDKDAVDLYNYAREKVIEEGGNFVVEGGVLEGAGFESGCYVKPCIAEVENHFAIV
                     QHETFAPILYLIKYKDIHEAIALQNDVPQGLSSAIMTTNMREAELFLSANGSDCGIAN
                     VNIGTSGAEIGGAFGGEKETGGGRESGSDSWKAYMRRQTNTINYSTELPLAQGIKFDL
                     "
     gene            6445..7704
                     /locus_tag="PLG24_03720"
     CDS             6445..7704
                     /locus_tag="PLG24_03720"
                     /inference="COORDINATES: protein motif:HMM:NF028181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3472 domain-containing protein"
                     /protein_id="HMZ24025.1"
                     /translation="MFIKKLLILYFSFFIGISVYGQQVLCAKIPGYTGYSIPNDKGIV
                     FDELLGCTQWTNENLTLQYNMQIEEKGDLILKFMLSNDGPSPAYISVKFGDKTREVKV
                     PPTGSHIQYILVDAGIFTVDYPGFYSVWIKPISKSGQYFPGIMNVQLCAPFADKINFP
                     TIPDRNAPTVNLTYLNPQKKKVSGMVVNALVPNNFDYTGTRIAAIGNEVFKVGMANET
                     KGRYIYLSWKKVKGYEIPQFQYSQFKSFFIDSSNEKVNRLVIPYNWSPDQPIAFSLSL
                     REDTCTGEHFWEAQVFNDKKKRWHTIGIVSGGKMDNLIKDWNSTIVNSDPNTGNVEHK
                     ALFSNQYFILADGSKYQVAKARFGHDVKGKKERKDYGAGIVNNSFWLSTGGFSFSQAT
                     YGRIYEVKLKPGVPSNEIFLASFDSAK"
     gene            complement(7770..7856)
                     /locus_tag="PLG24_03725"
     tRNA            complement(7770..7856)
                     /locus_tag="PLG24_03725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7820..7822),aa:Leu,seq:cag)
     gene            8347..9762
                     /locus_tag="PLG24_03730"
     CDS             8347..9762
                     /locus_tag="PLG24_03730"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HMZ24026.1"
                     /translation="MKSITVFEIIKVGIGPSSSHTMGPWNAAEMFLDLIREKKDIASV
                     REIFVEFFGSLAKTGIGHGTDIAGMLGLSGENFKTIDTSQIMEKADVIKSEGKIILGG
                     EKEIPFVYGKNLVLNKDKSLPFHPNGMIFKAIFNDGSELSQDYYSVGGGFVATQEEVS
                     IENYCVRTLHPCHHGDDILKYCNQLGVSVSDLIYINEESWQSREETNRKALEIWHHIK
                     ECIYHGVNTEGILPGGLNVVRRAAGLNRKLLGDKVYRNMEEWYNLIVDSEVAFTEINK
                     WISCFALAVNEENASFGRIITAPTNGASGVIPAVLMYSQAFTKYTGDDNIIRFLLVAG
                     EIGTLFKKNATISAAMGGCQAEIGVSSAMAAAGLTEIMGGTPAQVLMAAEISMEHHLG
                     LTCDPVGGLVQIPCIERNSMGAIKAITAANIAIESDPSKARVTLDEVIQSMWETALSM
                     NDRFKETSEGGLAIAVNIAEC"
BASE COUNT         3107 a         2185 c         2159 g         2705 t
ORIGIN      
        1 tgacggggca tacatgggta catgagccac acagggtcga cgcaaaagga agatcagcgt
       61 attgcctcat atcaagattc ggtgcgagga tggagccaat aggcccggct accgcagtat
      121 gatagctgtg gcccccactc ctcctatata caggacaggt attcatacag gccccacaac
      181 ggatgcattt gagtgaattg cgaaactgtt ccctggccaa ctgcttacta cgcccattgt
      241 ccacgatgac aatatgtatc tttctctctc tatccggctt gcggaaatgg ctgctataag
      301 tggtaatggg ttggccggta gcgctacgtg ccagcagacg cagaaaaata ccaagatcct
      361 tcctgcgggg aataatcttt tcgatgccca tacaggctat atgcaagtcg gcaagatggg
      421 cacccatatc ggcattgcct tcattggtac acacgacaaa ttcaccggtt tgcgcgatgg
      481 cgaaattgac gcctgtgatg gcggcccgac gacttaggaa tgcctcccta agatgctgtc
      541 gggcagcctc tgtaagtatc ttgggatcag agcaaccttt gggcgtgtcc agatgctccg
      601 caaaaagatc accgatctct tcttttttct tgtggatgca aggcagaaca atgtggctcg
      661 gtgcctcacc cgccaactgc actattcgct cacccaggtc ggtatcaatt acatcgatcc
      721 cctgcttgag aagatattca ttcaagccac attcctcggt caacatggac ttactcttca
      781 cgattctgtc tattccgttt cccttcaaaa gcccgtgcac aatcctgttg tgctcatccg
      841 catccaccgc ccaatggact acgaggccgt tttcaactgc tttttgttca aattccacca
      901 gatattcacc caaattggcc aatgtatggc ttttaatctc agaggcagct tgccgtaaag
      961 cttcccactc aggcaattga taagccatct tgtctcgctt cgcccttacc caccacagtg
     1021 tctcatcatg ccatccaaca cgggctgcat ccttgttgaa tgccgcagcc aacttgctgt
     1081 ggggttcgct gttgtgtcta ttattcaccg cttcaacgga tttcgtcatt taatatttca
     1141 gcgagatgca cgaccctgac agggcacttc cttcgcctca gcaagccttc aagatgcatg
     1201 aggcaggaca tatccgtgcc ggtgatgtat tgtgcgccat gacggatatg atcatcgatg
     1261 cgatcattgc ccatccttac agatacagtt tcttcgaaga cggaaaaagt accgccaaat
     1321 ccgcaacatt catcttcccg gtcgagtgca acaagttcca atccattcac catccttaga
     1381 agggaaccgg gtttggaaaa ccgattaccc ggcacttcag acatcgaact tagaccaaga
     1441 ccgcgaattg catgacatcc attatgcagg cctacccgat aaggacaact agcatctaag
     1501 gaagttatct ccataacatc tgtaagaaat tccgacaatt ccatgactgc agggccatcc
     1561 gattcattgc tcctgggcat gacatggtcc tttacatgaa gtacacaact accggatggg
     1621 gaaacaatgt actcatacct gtcaaaacac tgccgaaaga tgtcagcaca tccttctgcc
     1681 aaatattcat aaccggcatt ggccatcggc tgaccgcaac atgtctgatt ggatgggtat
     1741 tccaccgtac agcccaactt ctccagcaga tgcagagtgg cctttgcgac ctgtggaaag
     1801 aactgatcaa tataacacgg tacgaataat gcgactttca taatcaatgc ctaatccggc
     1861 aatacaaact taatcatttt cggaagaatg gattaaaaac ataaatgtac aacgaaatgt
     1921 acccttttga aaattaagtg atttcaaaac cagatcttta ccgatgaagt tccaacgtga
     1981 tataagtaag atttcataaa gtcaattcac aaagctatcc ggttgatttg agaataattt
     2041 tttgatatgc gtttgcatca aaatgattta cctaaacaat atttaaacta accatcatca
     2101 ggtaatacgt taaatataaa aataataatc cgggagaaac cattttcctc atcttatttt
     2161 acctttgaag gatgaaaaat ccgtttttca aatatcttgc tttcttgagc atcatgatga
     2221 ttccacttct ctttgcccga tgcaaggaag atgcgaatga tacaaatgcc ggtaaaaagg
     2281 aattgctccg gggcatagat atcagttttc tgcctcaaat caggcaatac ggtatttctt
     2341 tcaagaatca atccggccag gaaggcgact tgctcaaaat agtaaaggaa aatggtgtca
     2401 atctcgtcag gattagactt tggacgggta atcccgggaa acattccgga gaggatgaag
     2461 tgtatgccct tgccgaagag tgtcgcaatc agggattcaa gatcttactt gacctgcatt
     2521 attccgacac atgggccgac ccggggcacc agactattcc tgcttcctgg atcggcctgg
     2581 atattaatca actcaaggat tccgtgtatc gctatacttt ccgggtcacc tcgaagtgtc
     2641 gtcccgacta tatccagatc ggcaatgaaa tcaatgcagg tctactttgg ccaacaggcc
     2701 acatcagccg gcctgcctct atggacgaac tgttgaaagt cggttgtaat gcagcgcgag
     2761 atgcccatcc tgccataaaa atcctacttc actatgcagg tatcgatggc agcatcgatt
     2821 ttttcaaaaa gagaaatgaa accggcattg attatgatat tgctgccctc tcgtactacc
     2881 cctactggca cggaagcaca atagccgaca tgggaaatac cttgaatgac atacgttttt
     2941 acacaaagaa agaagtaatg attgttgaat ttgcctatcc atttaccttg aaatggagtg
     3001 accataccaa caatgtgatc ggtctggaaa gtcagttatc accgggctac ccagccactg
     3061 cagaaggcca ggcggtcttt gtacgtacca tacggacagt gtgtgagaaa tataaggcaa
     3121 agggtctctg ctattgggga ggggaatggg ttgcctttaa gggtgacacc tcctccaatg
     3181 gctcttcctg ggaaaatcag gcattgttcg actttgactt cagggcaaat cccgctttga
     3241 ctgaatacag gtaaacactg caatggatca attaggtatt acaatttgaa ttgaaatatc
     3301 catatttgca aaaaaatact tccaatggaa ttaaatgata aatgggtgtg ggtcaccggt
     3361 gcttcctcag gaatcggcaa ggctattgct atcgaacttt cttttcacaa agccaatctt
     3421 gtgctcatga gcagaaatgt cagtgaacta aaaaagaccg ccgatgcctg taatccaaat
     3481 gtcaatatca ttatacaacc tattgatttg gcagacaatg cctccataga tgaagcggtt
     3541 agggtactgc tgcatgacaa aaaaatccca ccacacatcc tgatcaataa cggaggtatt
     3601 agtcaacggg ccacagcgct ggacaccgtg gatgcagtga cccgcaagat attcgacatc
     3661 aactttttcg gcacggtata tcttaccagg gcactactgc caaggatgat tgaaatggga
     3721 ggagccagaa tcgcggtaat taccagcctg gttggaatca ttggaagccc caagagaacc
     3781 agctattctg ccagcaaaca cgctctgcac ggatatttcg acagcctgcg tgctgaattg
     3841 tacaaagaac agataggcgt cacattgata tgtccgggtt tcataagaac caatattagc
     3901 ctctcggcag tcaccggtga cggaactgca cagaatacaa tggatgaaaa gacaggcaat
     3961 ggcatggatc ccgccaaact ggccaaaatc gctgtccggg ggatcatgcg tgaaaaggaa
     4021 gaaatctata tcggtggcaa ggaaatactt gcaatatact taaaacgatt cgttccgacc
     4081 attttctcca aaatattgag aaagacgaag gtaacctgac ctacaagaaa cattaataaa
     4141 ttcagtgagg ccaaaaaacc gctactgtgc cggtcagata tccgtgtaaa tatttcgtat
     4201 cagatctttt caaccatagg tacaaaaaaa acatagtact ccaattatta tcagtacgcc
     4261 cttccggaac caaggtgaga ttgccggtat tccaacccta ttggcatgac ccaataaaat
     4321 taaactacca ttcaaacgat tgaaaattct tgtagaaagc cccctttatc ctgaagggaa
     4381 gactcatcta ttccgaaggg ataaataaag aaccatgttc aggatcttca tttatggatg
     4441 taggcgaaaa tacaggttgt caaactgaac gtaaagttgc tcaaaagaaa ttgataagta
     4501 tattatacag gtgccaaaat aacgaagacc taaaacattt taaccgttat taaaaaatca
     4561 ccctgaacat aaaatcagaa tatccattga aacaagggaa tataattgtt taatcggttt
     4621 tcttatcgta attttgcacc tcaaaaaaat aaacattaag atatggatat atcctttttg
     4681 aaaacattag gaatcaatga aataaatccc ggcacaacaa caggtacgat ttggtttgaa
     4741 cctgaagaat accttgacag ttattcacct gtaaatggca agctgattgg taaggccggc
     4801 attactacag aagctcaata cgaagacatt atcaacaaat caaccgaagc cttcaaattc
     4861 tggcgaaata ttccggcacc caaaagaggc gaaatagtgc gccaatatgg tgatgtactg
     4921 agggccaata aagatgctct tggccgcctt gtatcttatg aaatgggtaa aagccttcag
     4981 gagggctggg gcgaagtaca agaaatgatc gacatatgtg actttgcagt gggattatcc
     5041 cgccagctat atggtctaac catgcatagt gaacgtcccg gccatcgtat gtatgagcaa
     5101 tggcatcctc tgggcatcgt aggcatcatt tcagctttta actttcccgt agcggtatgg
     5161 tcatggaact ccatgattgc ccttatttgt ggagatgtat gcgtatggaa accttctgag
     5221 aaagttccgt tgagtgccat cgcctgtcat aaattacttg aagaagttct caggaaaaat
     5281 aatattcctg aaggagtaac ctgtcttatc aatggtgact acagaatcgg cgaattgatg
     5341 accaaggatc cgagagtagc ccttgtatcg gcaaccggta gtgtcaggat ggggaagatt
     5401 gtaggagcga cagtagctgc acgccttggt aaatctttgc tcgaattggg tggaaataat
     5461 gccatcatcg tcacccctga tgcagatctc aatattgtgc tcaccggagc attattcgga
     5521 gctgtaggta ctgccggaca gcgttgtacc actaccagaa gactcattat tcacgaaagc
     5581 cactatgaaa atgtcaagca aaaacttgtg aaggcatacg gacaactcgt catcggtaat
     5641 ccactggatg cgggagttca tgtaggtccc ctgatcgata aggacgcggt tgatttgtat
     5701 aactatgcaa gagaaaaagt aatcgaggaa ggaggaaact ttgtggtcga aggtggtgtg
     5761 ctcgaaggtg cgggctttga gagcggctgc tatgtaaaac catgtatcgc cgaagtggaa
     5821 aatcattttg ccattgtaca acacgagact tttgcgccta tcctgtatct gataaaatac
     5881 aaagatattc atgaagcaat agcccttcag aatgatgtcc cacaaggatt atcctctgcc
     5941 atcatgacga ccaatatgcg tgaagcagag ttgttcctgt ctgcaaacgg ttccgattgc
     6001 ggtattgcta atgtcaacat aggtaccagt ggtgcagaga taggcggtgc attcggcgga
     6061 gaaaaagaaa ccggtggcgg aagagaaagc ggctctgata gctggaaagc ctatatgcgc
     6121 cgacagacca ataccatcaa ctacagtacg gaattgccac tggcccaagg gattaaattt
     6181 gatctgtaaa tatcaaggtg ctgtcgatct gcttttttgt atatcgaccg cgcctaccca
     6241 ttttgccaac tatatttcag ttgcaactca aattatgctc ccaaacagaa aattaattgt
     6301 tttttaaaat tatacttaat ttattataaa aaattaatat ttgtttttag ctttgaacca
     6361 taattccatt tacgtatttt tgtcagcttt taggcctgct cttaatgtaa attttcactg
     6421 tttgcaagat atcggtttgg aaatatgttt ataaaaaaac tgctgatttt atacttctcc
     6481 tttttcatag gcatttctgt ctacggtcag caggtacttt gtgctaagat accgggctat
     6541 accggctatt ctattccaaa cgacaaaggc atcgtcttcg atgaattatt gggctgtacg
     6601 caatggacca atgaaaatct aacacttcag tataatatgc agatcgaaga aaaaggtgat
     6661 cttatattga aatttatgtt gtcgaatgac gggccctctc ctgcctatat cagtgtgaaa
     6721 tttggtgaca agacccgaga ggtgaaagtc cctcctacag gcagccatat ccaatatatt
     6781 ctggttgatg ccggaatatt caccgtggat tatcccggtt tttattctgt gtggatcaag
     6841 ccgatatcca aatccggcca atacttcccg ggcatcatga atgttcagct gtgcgctccg
     6901 tttgcagata aaatcaattt cccgactata ccagaccgca atgcccctac tgtaaatcta
     6961 acctatctta atcctcaaaa gaagaaagtc agcggcatgg ttgtaaatgc acttgtacca
     7021 aacaatttcg attatacggg aaccaggatt gctgcgatag gaaatgaggt tttcaaagtt
     7081 ggcatggcca atgagaccaa aggtaggtat atttatttat cctggaaaaa agtcaagggc
     7141 tacgagatac ctcaattcca atatagccaa ttcaaaagct tttttattga ttcttctaat
     7201 gaaaaggtta atagattggt tattccatac aactggagtc ctgatcaacc cattgcattc
     7261 tcactttcac ttcgcgaaga tacttgtaca ggggaacatt tctgggaagc tcaagtcttc
     7321 aatgacaaga agaaaagatg gcataccatt ggtattgtta gtggaggtaa aatggacaac
     7381 ctgatcaagg actggaatag caccatcgta aattcagatc caaataccgg caatgtagaa
     7441 cataaggctc ttttttcaaa ccaatatttt atactggcgg atggttcaaa gtatcaggtt
     7501 gcaaaagcac gcttcggaca tgatgtaaaa ggaaaaaaag aacgcaagga ttatggagcg
     7561 ggtattgtca acaactcatt ctggctaagt acaggtggct tctctttttc tcaggcaacc
     7621 tatggtcgca tctatgaagt taagttaaaa cccggtgtgc caagcaacga aattttttta
     7681 gcaagcttcg attcagccaa gtaaattaat tcaataacca acataaaaaa aataaagcgc
     7741 aggaaacact tcctacgctt tgttaaaact ggtgcccaag accggattcg aaccagcacg
     7801 tcctttcgaa cgctgcgacc tgaacacagt gcgtctacca atttcgccac ttgggccact
     7861 gatttaggat gcaagaataa tcataatttt taaaaacaac atcatttttt tacttaatta
     7921 tccataaaac aaccatacaa tttgccctgg aattacccca aattgcataa tatattgaaa
     7981 ataacgcact cgcaacctaa caagcatact gaaaggaatc aggcggaaaa ataaaaagcc
     8041 tcccaacgga aggcttttat aatattgact atattcctgt atttttttag gaaaatgagt
     8101 aagaaaaata tcgttatata tctatatatt cattcattca aaaaaacgct gcacgaagta
     8161 tcattttttt tccaattttt caattcggat tattcaacgc acccagatca aacatgccga
     8221 taacaacccg ctataactca atcaaactct gtattgttgt tgaatatatt cataaattgc
     8281 tatgttatac ttttgaattg atgtagattt gtcatcaacc aaatcatcat ttatattaaa
     8341 attaagatga aatcaataac tgtttttgaa attataaaag tgggaatagg cccatccagt
     8401 tcacacacca tgggcccctg gaatgccgct gagatgtttc tcgatcttat cagggagaag
     8461 aaagacatag catcagtgag ggaaatattt gttgaatttt ttggttccct tgccaagacg
     8521 ggaatcggcc atggtaccga tattgccgga atgctgggat tatcgggaga aaacttcaaa
     8581 acaatcgata catcgcagat tatggaaaaa gctgatgtta tcaaatcaga gggtaaaatt
     8641 atactgggtg gagaaaagga gattcccttt gtgtacggta aaaacctcgt actcaataaa
     8701 gacaaaagcc tgcccttcca tcccaatgga atgatcttca aagctatctt caatgatggc
     8761 agcgagttat ctcaggatta ttactctgtg ggaggaggat ttgtagctac acaggaagag
     8821 gtatccatcg aaaattattg tgttcgcacc ttgcatcctt gccatcacgg tgacgatatt
     8881 ttgaaatact gtaaccaatt gggagtttct gtttcagatt tgatctatat caatgaagaa
     8941 agctggcaaa gtcgggaaga aaccaatcga aaggcgcttg aaatctggca ccatatcaag
     9001 gagtgtatat accatggagt aaatactgaa ggcatcctac cgggagggct aaatgtggta
     9061 aggcgtgcag caggactaaa tcgcaaacta cttggcgaca aggtgtaccg aaatatggag
     9121 gaatggtata atctcattgt agattcagag gttgcattca ctgaaattaa taaatggatt
     9181 tcttgctttg ctcttgcggt caatgaagaa aatgccagtt tcggccgcat catcacagca
     9241 ccaaccaatg gggctagtgg tgttataccc gctgtactga tgtattctca ggcatttacc
     9301 aaatatacgg gcgatgacaa tataatccga ttcttactag tagccggcga aataggcacc
     9361 ctgtttaaga agaacgcaac aatatcagca gcaatgggtg ggtgtcaagc tgagatcggt
     9421 gtttcatcag caatggctgc agccggtctt acagaaatca tgggtggaac acctgcacag
     9481 gtactcatgg cggcagaaat atctatggaa catcatcttg gattgacctg tgatccggtt
     9541 ggcggccttg tgcagatccc gtgtattgaa agaaactcga tgggcgccat caaagccatc
     9601 accgcagcca atatagcgat agaaagtgat ccctccaaag caagagtcac tttagatgaa
     9661 gtcattcaat ctatgtggga aacagcactt agtatgaatg accgcttcaa ggaaacatcc
     9721 gagggaggct tagccatagc agtgaatata gcagaatgct aatatggata ttccggaata
     9781 agctgacccc aaatttagaa gaaaagaaat ctcagaaaca agttataatc tttcaacatt
     9841 ctgacattcc ggcataagct gcccaccctg aaagttcaat aagcatgagg caaattccgg
     9901 agcatgttga ccccctttct tgtgaaattt caaagaaaat gaggattgat gaaatgctgc
     9961 gtttagcatt ttgtagcgtg cgcacattgg attccggagc atgttgactc ctctgggcaa
    10021 tttgtgaggt cagaagcaag actgattccg gagcatattg aaccctggct tggattttgg
    10081 ttgtatttgg tttattaact aatacgaaag ttaaaaaata aatagaccaa aatttggtgt
    10141 attggattat ttgtat
//
LOCUS       DAOQQN010000063        10111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2433_length_10111_cov_5.120625, whole genome shotgun sequence.
ACCESSION   DAOQQN010000063 DAOQQN010000000
VERSION     DAOQQN010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10111
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2433_length_10111_cov_5.120625"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PLG24_03735"
     CDS             complement(<1..804)
                     /locus_tag="PLG24_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMZ24027.1"
                     /translation="MQKPLLITLLALLVSLNINAQDIHFSQFYASPLNLNPAMTGVMN
                     CTDRLTLSYRNQWASVLKSNAFNTFTASYDRKNTVGRYDYWGFGFNFWGDRAGELSFS
                     TLSPQISGSYSKRMGGSRKSAHYLSFGVMGGASNRSINFLKAKWGLQAKDGQWIANAP
                     SQENEANFNNSFWFGELGAGLLWFSVFDEETSFYIGGAYSHITRPNQSFLLEGVDVPL
                     YSKITAHAGGDFPMSDQISLVPGIVTFFQGPSFQVNGGTSLRLWLDKSRW"
     gene            1220..2167
                     /locus_tag="PLG24_03740"
     CDS             1220..2167
                     /locus_tag="PLG24_03740"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HMZ24028.1"
                     /translation="MKFFTKVPFTSDIRMKCSDLFLLSGSCFTENIGTIMQKLGLRVE
                     FNPHGILFNPLSVALSIENVIKKVAHNQDDILNYNGISFSWVHHTSVHAENEDELVRR
                     IDKEKNQFLLNLSNANYMIISWGTAFYYRLKESGRVVANCHKQPAALFNKIFAQPHEI
                     VDSYIRLVQNVLEVNPGLRIILTISPVRHIRDGFVENQQSKAALILAAKSLCEAFPET
                     ISYFPAFEIVMDELRDYRFYAEDMIHPGEVAVKYIWERYVEGYFDEYQREILKEAEDI
                     ARMKNHRIVFSGSENAIKFEDQKQKMIADFKARNPTFRL"
     gene            complement(2219..3193)
                     /locus_tag="PLG24_03745"
     CDS             complement(2219..3193)
                     /locus_tag="PLG24_03745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMZ24029.1"
                     /translation="MQKRQKIVFNQKTLLFETIPSKPFYKRYSIYGFSIILISILYFG
                     YNKINKSNIDQSLIAEIKKLEEKYSKLDNQLNQYSAHLDKLHKKDAQLYSVVMGVDPI
                     DESIWQAGTGGKHTIKTRLTNLLNLNINRIEDKANKLNHQLNLQNKSLDRLYRDAMAH
                     EKFLKCLPSIKPVKAEALNRDVTLLSGFGRRLHPIFKIWKKHTGIDFSCPKGTQIVAS
                     GEGTVLKINGNGRGYGKHVIIDHGYGFQTLYAHMSEITVKEGQKVERGTPLGKVGSTG
                     TATAPHLHYEVHKNGEPVNPIDYVLDGLSTEEYNQMLKQAAVEGKSMD"
     gene            3377..6028
                     /gene="alaS"
                     /locus_tag="PLG24_03750"
     CDS             3377..6028
                     /gene="alaS"
                     /locus_tag="PLG24_03750"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HMZ24030.1"
                     /translation="MIVSPIPSFEIRRIFLEFFRERGHQIVPSAPIVVKNDPTLMFTN
                     AGMNQFKDLFLGNSAIRHHRVADTQKCLRVSGKHNDLEEVGVDTYHHTMFEMLGNWSF
                     GDPANPSAGYFKKEAIQWSWELLHDKYGISADRIYATVFEGDPANNIPMDQEAYEEWA
                     KYLPRERILLGNKKDNFWEMGDTGPCGPCTEIHVDCRSDEEREQVEGSTLVNQDHPQV
                     IEIWNNVFIQFNRIKDGSLQPLPARHVDTGMGFERLVRVLQGKQSNYDTDLFEPTLRK
                     IEELSGAAYEGTMVKKDIAFRVIADHIRAISFTIADGQLPSNAGAGYVIRRILRRAIR
                     YYFSFLDCQEPLLASLVPVIAEQFKDVFPELHQQIDFVQRVIREEELSFLKTLDSGIR
                     RFEQIVAESAGTLPGEKVFELMDTFGFPVDLSVLMAQEKGRVIDMAGFEAALQLQKER
                     SRASAQIENEDWVVVNEVPEMSFVGYDNLNVETSIVKYRSFKAKDAIGHQVVLKETPF
                     YAESGGQVGDTGFLMFGHERVNVLNTKKENDLIIHTVDHLPQNAEQEVVAQVDEFRRN
                     KITVNHSATHLMHAALRQILGPHVVQKGSLVNDVHLRFDFSHFAKVTDEEIAQIEDIV
                     NEKIRENIPVIIKYMDKDEALRTGAMALFGEKYGNIVRVVIIDPAYSIELCGGCHVGY
                     TGEIGLFKITSESAVAAGVRRVEAMTGQAALQYLNAYVEEVRQVRQLMGNPKEVITKL
                     NDLIAENKSLRKEMEKMEADKALRLQHELSESVRRNSAGVNYIISRTELSDNKLLKNI
                     GFNLARESGDALVALINCYDEKVTIQINISESLADKKIYNAGELVRQLAPLIGGGGGG
                     QVGYATAGGNNVSGIDSVLEQLTQLVE"
     gene            6244..6678
                     /locus_tag="PLG24_03755"
     CDS             6244..6678
                     /locus_tag="PLG24_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HMZ24031.1"
                     /translation="MNIFVAKLSGATTADGLKRLFEQFGEVSDVKIIIDRETNMSKGY
                     GFVEMPNVAEGHTAIAELNEAEVDGSIIVVKESQPRTGEKSGFSGGGNRGGNRSFGGG
                     NKFKKPGFGGGRSFDKKPGFGRSGDSDHRKGGYHSREDRNSY"
     gene            6966..8429
                     /locus_tag="PLG24_03760"
     CDS             6966..8429
                     /locus_tag="PLG24_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HMZ24032.1"
                     /translation="MKIKTFIVLAIFCICTLPKHVFSINYTETKLERVAEDENEFDES
                     QIKIRLRKLNSSLDIRYTPEVKKFLQSYIKSYRSTSEQLLGRATMYFQYFDQALQKYN
                     LPSELKMISVLESSLNPNAISPAGAIGLWQFMPATAKDMGLDFNRVVDERRDPDKSAD
                     AAARYFLKLYSIFEDWNLTLAAYNCGPNRVQNAIDAAGGVKDFWAIRKYLPKETQNYI
                     PKFIAINYLFNYYNVHKLKPSFPELDLQITDLTSLYDKVDLKALSSKLGVSYDVMKVL
                     NPMYRQGYIPASNNPMNIILPMRYMNSFKSSIPRPDSKMLSVNESPLPSMSVGGNDYY
                     EMTYIAGPGENLERIAGRLGINSHLIRMWNGMTMNTIKEGQSLKLYILRNKQLDNSFA
                     KAELHPLTEQFVQVRTMMEDENFISLSHLDYTNDELVFTASSDQFVYHRLDERESIAD
                     LVSQYTGTTIQSILELNGFNRDNLPKPGDLIKVKKKQ"
     gene            complement(8525..8968)
                     /locus_tag="PLG24_03765"
     CDS             complement(8525..8968)
                     /locus_tag="PLG24_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HMZ24033.1"
                     /translation="MRHISKKEMRPFNPYRPFEQFFNNELANFWSGDNFLESPAFNII
                     EENDKYVIELAAPGLEKEDFNIEIKDQILTIKVQKQESKDAETTDNNTKYIKREFSFQ
                     QFTRSFTLDNTIAQNEIEATYDKGILKVSLKKVSNPEIIKKIEIK"
     gene            complement(9001..9390)
                     /locus_tag="PLG24_03770"
     CDS             complement(9001..9390)
                     /locus_tag="PLG24_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HMZ24034.1"
                     /translation="MTVIFKNVATPTMGQQTKASTRFIPLSNLINTGKGYILEMMVSG
                     FSKDDIEIDLKEDKLTIKGQMPKASADTIKFIRRGLKSGDFEATYTLSERIDKSAIDA
                     ECHNGLLRIVLPLKEEVIATPRKINIQ"
     gene            9775..>10111
                     /locus_tag="PLG24_03775"
     CDS             9775..>10111
                     /locus_tag="PLG24_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24035.1"
                     /translation="MNRFFVFLLSILFSFGAKAQVDSIHFQFQDTILKAGTTHTFLMT
                     VRENIQDALGFQFCLQFDPQNIGFKSIPKYYISGWTEDNMNIYPLGHPDYIACAVDVI
                     STPYTGKKLL"
BASE COUNT         2975 a         1781 c         2135 g         3220 t
ORIGIN      
        1 ccatctgctt ttatccagcc aaagtctgag agatgtaccc ccattgacct ggaaggacgg
       61 accttgaaag aaggtaacga ttccgggcac caggctgatc tgatcgctca tagggaaatc
      121 acctccggca tgagcagtta tcttgctgta taatggcacg tcaactcctt ccagcaggaa
      181 agattgatta ggtcttgtta tatggctata ggcaccgcct atgtagaaac ttgtctcttc
      241 atcaaacact gaaaaccaaa gtagtccggc tccaagttct ccaaaccaga atgaattatt
      301 gaagttggct tcgttttcct gtgaaggagc gttggcaatc cattggccat ctttggcttg
      361 caggccccac ttagccttaa ggaaattgat acttcggttg gaagcaccgc ccatgactcc
      421 aaaggataag tagtgtgcgg attttcggct gccacccatt cttttgctgt agcttccgga
      481 tatttgtgga gaaagtgttg aaaagcttaa ttcaccggct ctgtcacccc agaaattaaa
      541 tccaaatccc caataatcat agcgaccaac ggtattcttt ctgtcatagg aagctgtgaa
      601 agtattgaat gcattacttt ttaatacgct cgcccattga ttcctatagg acaaggtcaa
      661 cctgtcggta cagttcatca ctcctgtcat agcagggttg agattgaggg gggaagcgta
      721 aaactgagaa aaatggatgt cctgagcgtt gatattcaat gacaccaaaa gtgctagtaa
      781 tgttataagt agaggtttct gcatcgaaat ttaaaatttt aagcttgttt tcttgcggag
      841 taaaggtata aataatttgt atattaatag tgttaaaaat ttttttatca atttttctca
      901 atattatatt tcgtataacg cttcagaatt cttttatatt tactgaattt cgtttttgtt
      961 acgatatttt ttcggattaa attaaaattt gatatttatt gtttttccga ttttctattg
     1021 actgcaacat atagtccttt ttgactttta ttcaatcata gtagctaatt gtcacaggca
     1081 attttctata ttggacatcc tttcgttaat agcaaatata aagaacaatg atagatatta
     1141 tcatttatac aaatatatac atctgaaaat tagtaaacgc tgcattccat tttccgaatc
     1201 ccgatattaa ttttacacaa tgaagttttt taccaaagtt ccttttacgt ccgatatccg
     1261 tatgaaatgc agtgatctct tcctgctttc aggttcctgt tttaccgaaa atataggtac
     1321 gattatgcag aaattgggac tcagggttga gtttaatcca catgggatat tgttcaatcc
     1381 gttatctgtt gcattgagta ttgaaaatgt catcaaaaag gttgctcata atcaggatga
     1441 tattttaaac tataatggca taagtttttc ctgggtgcat catacttctg tgcatgctga
     1501 aaatgaagat gaattggtga ggaggataga caaagagaag aatcaatttc tattaaattt
     1561 atctaacgcc aattatatga taattagctg gggaacagct ttttattaca gattgaaaga
     1621 gtcgggaaga gttgttgcca attgtcacaa acagccggct gccttgttta ataagatctt
     1681 tgctcaacca catgagatcg tggattcata tattcgtttg gttcaaaatg ttctggaagt
     1741 gaatcccgga cttcggatta ttctgacgat aagccctgta agacatattc gagatggatt
     1801 tgttgagaac cagcaaagta aggctgcctt aattttggcc gccaagtctt tatgtgaggc
     1861 tttcccggaa acgatatcct atttccccgc ttttgagatt gtcatggatg aattgagaga
     1921 ttatcggttt tatgctgagg atatgattca tcccggtgaa gttgctgtca aatatatctg
     1981 ggagcggtac gtcgaaggat attttgacga ataccaacga gaaatattaa aggaggctga
     2041 agatattgcc aggatgaaaa accaccggat agttttttcc gggtctgaaa atgcaattaa
     2101 gtttgaagac caaaaacaaa aaatgatagc tgattttaaa gcaagaaacc ctactttcag
     2161 attataatag gatctgataa agaatttacc aagcgattaa gaagtgaaaa atggctgatt
     2221 aatccattga ttttccttct acagctgctt gtttaagcat ttggttgtat tcttcggttg
     2281 acaggccatc aagtacatag tcaataggat tgacaggctc tccattttta tgtacttcgt
     2341 aatgtaagtg aggcgcagta gctgtacctg ttgagccaac cttgcccaga ggggttccac
     2401 gttctacctt ctgaccttcc ttaaccgtaa tttcagacat atgagcatac agagtctgga
     2461 agccataacc atggtctatt atgacatgtt tgccataacc tcgtccattg ccattgattt
     2521 ttagcacagt tccctcgccg gaagctacaa tctgtgtacc cttgggacaa gaaaaatcaa
     2581 ttcctgtatg ttttttccaa attttaaata tcggatgcag ccgtctccca aaacctgata
     2641 gtaaggttac atcgcgattc aatgcttctg ccttcaccgg cttgatactt ggcagacact
     2701 tgagaaattt ttcatgtgcc atggcatcgc gatacaatct gtccaaggac ttattttgta
     2761 agttaagctg gtgattgagt ttatttgcct tgtcttcaat gcggttgatg ttgaggttta
     2821 aaaggttggt caatctggtt ttaatggtat gcttaccacc tgtacctgcc tgccagattg
     2881 attcatcaat aggatcaact cccatcacaa cagaatacaa ctgagcatcc ttcttgtgca
     2941 gtttatccaa atgagccgaa tattggttga gttgattgtc cagctttgaa tatttttctt
     3001 ccagtttttt aatctctgca atcaaagact gatcgatgtt gcttttattt attttattgt
     3061 acccgaagta tagaattgaa atcaaaataa tggagaaccc gtagatactg tatcttttgt
     3121 aaaatggctt tgatggaata gtttcaaaca gcagtgtctt ctggttaaaa actatttttt
     3181 gtcttttctg cattttccta ataatggttt atttactttt gcgcaaaatt tatttcttag
     3241 gttaaatgaa cacgggatgc ggcaaaagta caaatgtggc ttcatttttc aaaggaattg
     3301 aaaaattttt atacaaaaaa tatatatatg ttttcttttt aatatatata aaaggcattt
     3361 taacaaaatt taaaatatga tagtgagtcc tataccttca ttcgaaatca ggcgtatttt
     3421 tttggaattc ttccgcgaaa gagggcatca gatcgttcct tctgctccaa tagttgtcaa
     3481 gaatgatccg accttgatgt ttactaatgc cggaatgaat cagtttaagg atcttttcct
     3541 tggcaatagt gcaatcaggc atcatcgtgt cgctgacaca cagaagtgtc ttcgtgtcag
     3601 cgggaagcac aacgatttgg aagaagtggg tgtggatacc tatcatcaca ccatgtttga
     3661 gatgcttggc aactggagtt ttggcgatcc ggccaatcct tctgccggtt atttcaaaaa
     3721 agaggccata caatggagct gggaattact gcatgacaag tatggtattt ctgcagatcg
     3781 gatttatgct actgtttttg aaggagatcc ggccaataat attcctatgg atcaggaagc
     3841 gtatgaagaa tgggcaaaat atttaccccg ggaacgtatc cttttaggca ataaaaaaga
     3901 taatttttgg gagatgggcg atacaggccc ttgtggtcct tgtaccgaga tacatgtgga
     3961 ttgcagatca gatgaagaaa gggagcaggt ggaaggcagt acattagtca atcaggatca
     4021 tccgcaagtt attgagattt ggaacaatgt ctttattcag ttcaatagga ttaaagatgg
     4081 tagtttgcag ccccttccgg cccgccatgt tgataccggt atgggatttg agcgactggt
     4141 ccgggttttg caaggcaaac aatccaatta tgatacggat ttgttcgaac ccacattgag
     4201 aaaaattgaa gagctttcag gggctgccta cgaaggtaca atggttaaga aagatatcgc
     4261 attcagggta attgcggatc atattcgtgc catatcattt accattgccg atggacagct
     4321 tccctccaat gccggtgcag gttatgttat aagacgtatt ctgcgtagag ctatcagata
     4381 ctatttttcc tttcttgatt gtcaggagcc attgttggca agcctagttc cagtaattgc
     4441 ggagcaattt aaggatgttt ttccggagct tcatcagcaa attgattttg tccagcgggt
     4501 aattcgcgag gaagagcttt catttctgaa aaccctcgac agcggaattc gcaggtttga
     4561 gcagattgta gcagaatcag caggtacatt gcccggtgag aaagtatttg aactaatgga
     4621 tacatttggt tttccggtag atctttcagt gttgatggct caggaaaagg gtagggtgat
     4681 tgatatggca ggttttgaag cagctcttca gttacagaag gaacgaagca gggcttctgc
     4741 tcagattgaa aatgaggatt gggtagtggt caatgaagtc cctgaaatga gttttgtagg
     4801 gtatgacaat ctgaatgtcg agacatccat cgtaaagtat agaagtttta aggcgaaaga
     4861 tgccattggg catcaggtgg tgttgaaaga aacaccattc tatgctgaaa gtggtggcca
     4921 ggtgggtgat acgggatttt taatgttcgg ccacgaaagg gtcaatgtat tgaacactaa
     4981 aaaagaaaat gacctgatta ttcacacggt cgaccatctt ccgcaaaatg ctgagcagga
     5041 ggttgtcgct caggttgatg agttcaggcg gaataaaatt accgtcaatc actctgccac
     5101 gcatttgatg catgctgcct tgaggcagat cctgggccca catgtagtcc aaaaaggtag
     5161 tctcgtcaat gatgttcatc ttcggtttga cttttcccat tttgccaagg taacagatga
     5221 agaaattgct caaattgaag acattgtcaa tgagaagata cgggaaaata ttcctgtgat
     5281 catcaagtac atggataagg atgaagcact ccggacaggt gctatggctc tcttcggtga
     5341 gaagtatggc aacattgtcc gagtagtgat aattgatcct gcatacagca ttgaattgtg
     5401 tggcggttgt catgtaggat atacaggaga gatcggcctg ttcaaaatca cttccgaatc
     5461 ggctgtcgct gccggtgtga gaagagtaga ggcgatgacc ggccaagctg cattgcaata
     5521 cttgaacgct tatgtggaag aggttaggca ggtacgccaa ttgatgggca atccaaaaga
     5581 agttatcaca aagcttaatg atttgattgc agagaataag tcactcagaa aagaaatgga
     5641 gaaaatggag gcagacaaag cactccgatt acaacatgaa ttatcggaat cagtgcgaag
     5701 gaacagtgcc ggagttaatt atataataag caggactgaa ttgtcagaca ataagttatt
     5761 gaagaacata ggattcaatt tggctcgtga atcgggcgat gctttggttg ctttgataaa
     5821 ttgctatgat gagaaggtca caatccagat caatatatcc gaatcactgg cagacaagaa
     5881 gatctataat gcgggcgagt tagtgaggca attggcgcca ttgattggtg gaggcggggg
     5941 tggccaggta ggctatgcta ccgccggggg gaataatgtg tcaggaatcg attccgtttt
     6001 ggaacaactt actcaactgg tagaatgatt gaaaaaccgt tgggaagtat tgatatttag
     6061 aatatttcca aagaagaaag tattttttaa taaagcataa ttaaatgttt tataatcaaa
     6121 taaagcactt atctttgcat ggaattaatc accggcttgc tattaaatcg acgttttcgt
     6181 ttatttcaaa atcattccgg gtcgatttta ttaatgtttt aactaatttt ttaatttttt
     6241 caaatgaaca tttttgttgc taaattaagc ggtgctacaa ctgctgatgg acttaagcgc
     6301 ctttttgaac aatttggtga ggtcagtgat gtaaaaatta ttattgatcg tgaaaccaat
     6361 atgtccaaag gatatggctt tgtcgaaatg cctaatgttg ctgaaggtca cacggcaatt
     6421 gcagaactta acgaagctga ggtggacggc agtattattg tggtaaagga atcccaacca
     6481 agaaccggtg aaaaatcagg attttccgga ggtggaaatc gtggtggaaa cagaagtttc
     6541 ggcggtggga ataagtttaa aaaaccggga tttggtggtg gaagatcctt cgacaagaaa
     6601 cctggttttg gccgtagtgg tgatagcgat cacagaaaag gcggatatca tagccgggaa
     6661 gacagaaact cttattaatc cgcattaaag aaagaaagaa gcctgccata atttatggcg
     6721 ggcttttctt ttttaacata gaatagtaga tatattgatc tttaaatgta aatcctgtaa
     6781 atgcctggaa agcaggcagg cgtttctgcg actgtaggtc aatgtgtttc atattctaaa
     6841 aatataatat atgttaatat tttttctttt tctgctttaa actcgtacgg agactgaatt
     6901 tgtcctactt ttgcctttgc aatacgttgc actaggaatt tattttgtta actaaattac
     6961 tgacaatgaa aataaaaact ttcattgtac tagcaatttt ctgcatttgt acattaccaa
     7021 agcatgtatt ctcgataaat tatactgaaa caaaactcga aagagttgcg gaagatgaaa
     7081 atgaatttga tgaaagtcaa atcaaaatac ggttaagaaa gcttaattcc tcccttgata
     7141 tcagatatac cccggaagtt aagaaattcc ttcagtccta tataaaatct tatcgaagca
     7201 cgtccgagca attgctgggt agagcgacga tgtattttca gtattttgat caggctctcc
     7261 agaagtataa tttgccctct gagttaaaga tgatttctgt tctggaatcc tcgcttaatc
     7321 ctaacgcaat cagtcccgct ggtgcaattg gactatggca gtttatgccc gcaaccgcaa
     7381 aagatatggg tctggatttc aatcgggttg ttgatgaaag gagagatcct gataaatccg
     7441 ccgatgctgc agcccgttat tttttaaagc tgtactctat atttgaagat tggaatctta
     7501 cccttgcagc ttataattgc ggacctaaca gggtacaaaa cgctatcgat gctgcaggag
     7561 gtgtcaagga tttttgggca atccgaaagt atcttcctaa agagactcag aattatattc
     7621 caaagtttat tgcaatcaat tacttattta actattacaa tgtccataaa ttaaaacctt
     7681 ccttcccgga gcttgatctg cagattacag atctgactag tttgtatgac aaagttgacc
     7741 tgaaggctct ttccagtaag ctgggtgtat cctatgacgt tatgaaggta ctgaatccta
     7801 tgtatcgaca gggttacata cctgcttcca acaatcctat gaatatcatt ttacctatgc
     7861 ggtatatgaa ttcttttaaa agttcaattc cacgtccgga tagcaaaatg ttatcagtaa
     7921 atgaatcccc cttgccctct atgtccgtgg gtggaaatga ttattacgaa atgacctaca
     7981 tagccggtcc tggtgagaat ctggaaagaa ttgccggtcg actcggcatc aatagtcatc
     8041 tgatcagaat gtggaacggt atgactatga ataccatcaa ggaaggtcag tcgttgaagt
     8101 tatatatact tagaaacaag caattggaca attcgtttgc taaagctgaa ttacatccat
     8161 tgactgagca atttgtccag gtaaggacta tgatggagga cgagaatttc atttcgttat
     8221 cgcacttaga ttacacaaat gacgaattgg tctttactgc ttcatctgat cagtttgtat
     8281 atcatcgact tgatgagcga gagtcaatag ctgatttggt aagtcaatat accggaacta
     8341 ccatccagag tatccttgag ttgaatggtt ttaacagaga taatctgcca aaacccggag
     8401 atttaattaa ggtgaaaaag aaacaataaa aaaagcccgg aagtccgggc tttttcatta
     8461 ttacttcaag gtttgttgct acatccaatt ttatagattg atagcatatc cgggatatca
     8521 atgcttattt gatctcaatt ttcttgatga tttccggatt ggaaaccttt ttcaacgaaa
     8581 cctttaatat tcctttgtcg taggttgcct ctatctcatt ttgagctatt gtattatcga
     8641 gggtaaagct gcgtgtaaat tgctggaaac tgaattccct cttgatatac ttcgtattat
     8701 tgtcagtggt ctcagcatct ttcgactctt gtttttggac cttgatggtc agaatttggt
     8761 ctttgatttc aatattgaaa tcctcttttt caagtcccgg tgccgccaat tcaattacat
     8821 acttgtcatt ctcctcaata atgttgaaag cgggagactc aaggaaattg tcgcctgacc
     8881 agaaattggc cagttcgttg ttgaaaaatt gttcgaaagg cctgtaagga ttgaaaggcc
     8941 tcatctcttt tttgcttatg tgtctcatga ttattaatga tttaagaaat gaatgaatta
     9001 ttattgaatg ttaatttttc tgggagttgc gatgacttcc tccttcaggg ggagaacgat
     9061 tctaagcaat ccattatggc attctgcgtc aatggcgctc ttgtcaatcc tttctgaaag
     9121 agtataagtt gcttcaaaat ctcctgattt gagtcctcgt ctaataaatt tgatggtgtc
     9181 agctgatgct ttaggcatct ggccctttat ggtaagtttg tcttctttta agtcaatctc
     9241 tatatcatcc ttagaaaatc cgctgaccat catttccagg atatatccct tcccggtgtt
     9301 tataagattg cttaatggaa taaaccgagt agatgcctta gtttgttggc ccattgtcgg
     9361 agttgcaaca tttttgaata ttactgtcat ttttttatta ttttgttaca ctcacaatat
     9421 tgcaatattt gttcctttga tttaaaagtg cctaaaagtc agtttgttat accaaaacag
     9481 tgacataatg acacttaata agaaaacagt gcgccaaaat gacgtatttt ttttagttgt
     9541 ttaatattta aatgaaattt acccaatatt acgaccagga ggctctaagc tcattcgtat
     9601 tatcattaaa attaatgtgt atttatataa ttaacttata tttgcaagtt ggattatcga
     9661 aagattttta tatttttgca tttattttcg catcttgagg cagcacattt ataaaaatca
     9721 tagtatatct aagcgccctt agtattgaat taatttatac gcaaaaattt agttatgaac
     9781 agattttttg tttttttact tagtatcttg ttttctttcg gagctaaagc ccaggtagat
     9841 tccattcatt ttcagtttca ggatactata ttgaaagccg gtaccaccca tacttttttg
     9901 atgactgttc gggagaatat tcaggatgca ttgggatttc aattttgtct tcagttcgat
     9961 ccgcagaaca tcgggtttaa gagtatcccg aaatattata tttcaggatg gacagaagat
    10021 aatatgaaca tttatcccct gggtcatccg gattatatag cttgtgccgt ggatgtgatt
    10081 tctactcctt acacgggtaa aaaactactt g
//
LOCUS       DAOQQN010000064        10110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2434_length_10110_cov_5.872899, whole genome shotgun sequence.
ACCESSION   DAOQQN010000064 DAOQQN010000000
VERSION     DAOQQN010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10110
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2434_length_10110_cov_5.872899"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PLG24_03780"
     CDS             complement(<1..355)
                     /locus_tag="PLG24_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HMZ24036.1"
                     /translation="MEMQDSEFARAIRQGIPSTLPEPVLRDPLVAHAPVRNLIGVLTP
                     EERKLAIANALRYFQPEWHPVLAPEFADELDTYGRIYMYRFRPQYEMKARPVEEYPAL
                     SPQAAAIMLMIQNNLD"
     gene            497..1090
                     /locus_tag="PLG24_03785"
     CDS             497..1090
                     /locus_tag="PLG24_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24037.1"
                     /translation="MKNTLLLTTILSFILIVPAIGQKVKSEGNLDFLKGVKEVTLAYK
                     YDKMAVGKYKDGNEYVAKKTKELNEKEKGKGDDWAKKWVSDRSERFEPSFENLLSEYC
                     KGIDFDQNNKSEYTMTLETVFTEPGFNVGVMRQNAYINVIVHFTDKAGKELATLSIDK
                     IPGRDVFGYDFDTGQRIEEAYSKAGKTIGKYLTKKVE"
     gene            complement(1294..2232)
                     /locus_tag="PLG24_03790"
     CDS             complement(1294..2232)
                     /locus_tag="PLG24_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HMZ24038.1"
                     /translation="MRKIATIESIGSSTRIEGATLTDQEVKDLLKDLKITELKTRDEQ
                     EVIGYHEVLELIYSNYSEIKLSENYIKQLHQMLLKYSNKDERHRGTYKFLSNKVMATY
                     PAGEQRTIFATTDPALVASEMKELVGWTNETLKQKTIHQLIVIGSFIYDFLSIHPFQD
                     GNGRLSRLLTTLCLLQNDYSFIQYISFENHIEQNKKAYYEALMNGQKCRGTDNERIDL
                     WLIFFLESLKTLTEKLEKKYDVFKSKGGYLNERQKLIKDFIMSNQPNKVSDLANHFPE
                     IRLSTLKKDLQYLRVEQVLIMIGKGKGSVYVLTEKE"
     gene            complement(2233..2367)
                     /locus_tag="PLG24_03795"
     CDS             complement(2233..2367)
                     /locus_tag="PLG24_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24039.1"
                     /translation="MDKFIQKLNFDFVTNQQIIQLIGYIDGFKGKWNIAEKQENRYLK
                     "
     gene            complement(3034..3861)
                     /locus_tag="PLG24_03800"
     CDS             complement(3034..3861)
                     /locus_tag="PLG24_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24040.1"
                     /translation="MKWDFIKGIYEPFIGIDSFCFAFTKKGIHHSGLLCSGISNENLW
                     EMILFYGKCRHGEYIKMLSDIRIKAYQNEGGFSKMSKFANMVEKNLYIIAGCNGADKT
                     TASFTILPEIIECKEFVNADEIAKGLSPFQPEKVAFEAGRIMINRINELLINGETFAF
                     ETTLSTRSYRQKIETAKKKGYTTTLLFFWLKNVDLAKERVITRVLEGGHNIPPDVIER
                     RYLKGIQNLFNLYLPIVDGAFIFDNSYGYPELIAQQTLGEKVEIINTEKYNELKSYL"
     gene            complement(4107..5357)
                     /locus_tag="PLG24_03805"
     CDS             complement(4107..5357)
                     /locus_tag="PLG24_03805"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ24041.1"
                     /translation="MAIVQNVRHLFVNAYSGIKREVWLLSLIMLINRSGALVFPFLSI
                     YLTRHLGFSEIQAGWVLSAYGVGSMVGTLTGGWLTDKYGSFRTQFGALVLSGIGWIIL
                     AHITEYHHLLMLAFIQSSIGDIFRPANNSSITLFTDAGNRTRSFSLNRMAMNLGYSIG
                     PAIGGIFATISYTLLFWGDGLSCIFSSIAFFIIFRRYFTKKGPVEEDRPEIPVRGETT
                     GPLKDTIFLVFVISVILFATVFFQMLFTLPIYYAQHYKIGEAHIGTLLAVNGLFVFVS
                     EMAFVHYVGNRIENHKLIIAGVLATGLSFIMLNWVHHSAWLIVAMIVLSLAEILSMPF
                     MQTYVANRATQSNIGRYLALYSFAFSVAFIFAPPLGMWTIKNFGFHMLWNGCMAVAAI
                     NTVVIYFVFKADKHRKASLKSERG"
     gene            5680..7539
                     /locus_tag="PLG24_03810"
     CDS             5680..7539
                     /locus_tag="PLG24_03810"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HMZ24042.1"
                     /translation="MSIFRFRLSAIKQWPDIFKNLFSNYFLVVTTALILAGLWIYQIE
                     AGDKLSFFASHNVSRILFSLGLCIPAFLALHVLVNKQNQKILYFTLGLGTIILMLGIY
                     YVNYELRADKQFYRTLALFLAFHGLLSLSAYRKYSSVRDFWQFNTNLLLRTIGSLFFT
                     LVLYLGVIAAIAALNNLFGVHISYNLYFDLMIVALVVYNTFFVLAGMKTSFDESGSEF
                     QYPLSLKVFTQFVLIPLMALYAIILYAFSIKIIVLWSLPKGWIAYLVLLFSIAGILGF
                     LLIYPIRNSTEKKWVRIYSKTFFIALVPLILLLHVSIWTRIGQYGFTIHRYVVAILAI
                     WLTLIPGYFLLSKKDNIIVIPASLTALFLLACFGPWGFDAISQKSQQNRIEKIMHDAQ
                     IQDYYKKGKINEYKANDKVTTELNSIIDYLLLNHGARSMKGFDIASWDKIADSIIDRQ
                     QKFFEEPKMEKGDTAISNSINASSFYSSPNSYTFLKEINVPQSGGYNMARFDYSYSPL
                     QNQIEIISYSRLVPFDLNISFKDQNNIITLDTSGFKLNINGKRITADAEKTVIDALNK
                     CGTACFNAQSPIIVTGKQMDIEYRIIINSLSGKIISDQKYEFENAMGYLLFNQ"
     gene            complement(7533..8738)
                     /locus_tag="PLG24_03815"
     CDS             complement(7533..8738)
                     /locus_tag="PLG24_03815"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HMZ24043.1"
                     /translation="MNCFRLILLCLLPVFAFSQPCSTGCDGKRYIEYVFPEYEITSMK
                     YARPIPENFDLYLDVYQPKGDQLEKRPMILFVHGGGFVSGSKADMAGFCIDYVKRGFV
                     TASIDYRLMFGIPDQAGFIRGVVKAMNDFKAAYRFFKVDASGANQFRIDTTKMMIGGY
                     SAGAVTVLHSAYLDSGDYVPPIILNEINAQGGWKGNTGDTENLSHREDDILGVFNYAG
                     ATFTKELFDEGDPFLMSYHGDIDNTVPIDSADFFGFATAYGSRVLHPKCAEKGIPQTL
                     EVVPGGGHTEIFTDPNLWPYLLEFVGKAKDIYYPRICGTPANTGTLADEIAATAFPNP
                     SSSKIKITFDRNVDGVLIYDRYGRLMSKHTPSGHVIEVEKHELIPGIYFAIPVIDGRQ
                     YRAVKIEFY"
     gene            complement(8929..9603)
                     /locus_tag="PLG24_03820"
     CDS             complement(8929..9603)
                     /locus_tag="PLG24_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HMZ24044.1"
                     /translation="MTDYYQILGLPAGASATEVKKAYHKLALKYHPDKNPNNTNAEYI
                     FKQITEAYNCLSNPADKLLYDMQFSVQQQSLAHVYRDDKKYQEAKKQQVTPTAMLTIF
                     QNLRREVARVDKSKIDPGRYFVSINNLLSIANIDYFLSVGDIATNRDIVFEAMACCKP
                     LPYFFVNRLVPRLVKLAGADNETIVKIYGFDRRHRMREKWIRYLPLILFLMYILINVS
                     LFWKGC"
     gene            complement(9886..>10110)
                     /locus_tag="PLG24_03825"
     CDS             complement(9886..>10110)
                     /locus_tag="PLG24_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ24045.1"
                     /translation="NSHTDCRGSEAYNKTLSARRAKSVVDYLTANGINRNRLSSRGFG
                     EMMPLAKCPCEKCTEEEHQLNRRTTFQLVR"
BASE COUNT         3064 a         2073 c         1913 g         3060 t
ORIGIN      
        1 aatccagatt attctgaatc ataagcataa tagcggctgc ctggggcgaa agtgccggat
       61 attcctccac cggccgggcc ttcatttcat actgaggcct gaagcgatac atgtagattc
      121 gcccataagt atccaattca tcggcaaatt caggtgcaag aaccggatgc cattccggtt
      181 ggaaatagcg caaggcattt gctatggcaa gttttcgttc ttccggggtc aataccccga
      241 ttaagtttct cacgggggca tgagcgacca atggatcccg aaggacaggc tccggcaatg
      301 tcgaaggaat accttgccgg attgcccgcg caaattctga atcttgcatt tccatttatt
      361 ccaaattttt atgttgcaag ttaagcaatt tttaaaaagt cggaatttag aataagatca
      421 gagtataatt taatagggct attcaaagaa aatatttatc tttaatactt gaaaaacttt
      481 taattaataa atgcttatga aaaatacact tttattgaca actattctgt cttttatcct
      541 tattgtacct gccattggcc aaaaagtaaa atcggaaggc aatcttgatt ttttgaaggg
      601 tgtgaaagaa gtgacgctcg cttataagta cgataagatg gctgtaggca aatataaaga
      661 cggcaatgag tatgtcgcaa agaaaacgaa ggagttgaac gaaaaggaaa aagggaaggg
      721 cgatgattgg gccaaaaaat gggtctctga ccgctcagag cgttttgaac ccagttttga
      781 aaatctgttg agtgaatatt gcaaggggat cgattttgat caaaacaaca agtcagaata
      841 caccatgact ttagagacgg tgttcactga gcccggattc aatgtcggag tgatgcgtca
      901 gaatgcatat atcaatgtca tcgtacactt caccgacaag gccggaaaag aattagccac
      961 cttgagcatt gataaaattc cgggacgcga cgtgttcgga tacgatttcg acaccggaca
     1021 acgtatagaa gaggcttatt caaaggcggg caagaccatt ggtaaatacc tgactaagaa
     1081 ggtagagtaa ttgattctta ccacgcaaaa gaccatccgg tcatcacttc ttgcgaggta
     1141 atctgaatgg ctatcggaat ttcactttgc tgattctgca aaagtaattt cggtagccat
     1201 ttttttggaa tcacattttt cctgaaagtt tcaaacgaag ctcttaaccc gctcgcttgt
     1261 aaaacccttg ttaacggttc gtgcagtgtt tagctactct ttttcagtca atacatacac
     1321 gctccctttt ccttttccaa tcattatcag cacctgttca actcgtaagt attgcaggtc
     1381 tttttttaag gtgctcaacc tgatttcggg aaagtgattt gctaaatcac ttactttatt
     1441 aggttgattg ctcattataa aatctttgat taacttttgt ctctcattca gataacctcc
     1501 tttggatttg aaaacatcat attttttctc caatttttcg gttagtgttt tcaaactttc
     1561 cagaaaaaaa atcaaccaca agtctatccg ttcgttgtct gttccccgac atttctgtcc
     1621 gttcatcaaa gcttcgtaat aggctttttt attttgctcg atatggtttt caaaagaaat
     1681 gtattgaata aacgaataat cattttggag caaacaaagc gttgtaagca atctcgaaag
     1741 tctgccgttt ccgtcttgaa atgggtgtat gctcaaaaaa tcatagataa aactgccaat
     1801 tacaatgagt tgatgaattg ttttttgttt caaagtttcg tttgtccatc caactaattc
     1861 cttcatttct gatgctacca atgccggatc ggtagtggca aaaatcgtcc gctgttcgcc
     1921 tgctggataa gttgccataa ctttgttaga aaggaacttg taggttcctc tgtggcgctc
     1981 atctttattg ctgtatttca gcaacatttg gtgtagttgt ttgatatagt tttccgaaag
     2041 tttgatctcg gaataattgc tgtaaatcaa ttctaaaact tcgtgatagc ctataacctc
     2101 ctgttcatca cgggttttta gttctgtaat tttaaggtct ttcaataggt cttttacttc
     2161 ttggtcagta agcgttgctc cttcaattct agtagatgag ccgatacttt caattgtggc
     2221 aattttccgc aattatttca gataacggtt ttcctgcttt tcggcaatat tccatttacc
     2281 tttgaagccg tcaatgtagc caatgagttg tataatttgc tgattggtaa cgaaatcaaa
     2341 attgagtttc tggatgaact tatccatata ctatctgtat tttatccaaa aaacaagtga
     2401 aaaatgggta ttccggagca aagtgagcca ctgattccgg aacaacgaga gccacaatga
     2461 agcattcgat aatttggaac gtgcaaagca aaaggtgtta acccctatga atggctcgca
     2521 gatgccctca ataaaatcct atctcagcct gtcaaccgga tacatggact gctccaagga
     2581 tatataccga aaaatgaagt gtagttgccc ggtgggttac aataatatat gtgaggtttt
     2641 tgtgtaaata gaagaaatgt cactaaatta actttctgcg gattgcttac gcctaaccgg
     2701 caaattgtat agggagcgcc attttaacat catggacgct aagtgtaagc gcagtcatgg
     2761 tcgtccatgg tccatgattg aatagaatta aaatcattca tttttgtatc ctggatctcc
     2821 gggtttcgat cgaagccgtc aacctgctct cttgccaatt tgtttgttgg gcgattgtgc
     2881 ctaattctca cggtttaatt ttcacgattt tatcgccttt cataaccaca agtacactat
     2941 ttttttgccg tttatattca ataaggttcg tgtacatttt ttccaaacct tttagtagtt
     3001 tgtccttaaa ttctatctgt ttttctttct tggtcataag taacttttta attcattata
     3061 tttttccgtg ttaataattt ctactttttc tcctaatgtt tgttgagcaa ttaattcagg
     3121 ataaccatac gaattatcaa atatgaatgc gccgtcaaca atgggtaagt ataaattgaa
     3181 aaggttttgt atgcctttca agtaccttct ttcaataacg tcagggggaa tattatgtcc
     3241 tccttctaag accctggtta tcactcgttc tttggccaga tcaacatttt taagccagaa
     3301 aaacaataat gtcgtagtat atcctttttt cttagctgtt tctatttttt gtctataact
     3361 tcgggttgag agtgttgttt caaaagcaaa ggtttctccg tttatcaaaa gttcattaat
     3421 ccgatttatc attattcgcc ctgcctcaaa tgcaactttt tcaggttgaa atggagaaag
     3481 accttttgct atttcatcag cgtttacaaa ttctttacat tctattattt ctggcaaaat
     3541 cgtaaaagaa gctgttgtct tatcggctcc gttgcaacct gcgattatgt aaagattttt
     3601 ttctaccata ttcgcaaatt tactcatttt actaaaacca ccttcgttct gatacgcttt
     3661 tattcgtata tcgctcaaca tctttatata ctctccatgc cgacattttc cgtaaaaaag
     3721 tatcatttcc cacaagtttt cgttggatat tccggagcaa agtaagccac tatgatgtat
     3781 tcctttcttg gtaaatgcaa agcaaaagga atcgatccct atgaatggct cgtagatacc
     3841 cttaataaaa tcccatttca tcctatcaac cggatacatg gattagtccc gggagatata
     3901 ctgaaaaatg aagagtagtt gccctgtggg ttaccttttg ctaaaaaatc tgtagtacac
     3961 aaatcagaac atccccctcc ggaaacttcc aatctacaca ggatctcatc tgcgtaagcg
     4021 caacccgggc taaacagcta agaccttaca tttcaataat aaattatact tattctctac
     4081 ctgattttcc ccggaagtta taatatttat cccctttcag atttcaagga ggccttcctg
     4141 tgcttatctg ccttgaatac aaagtaaata acgacggtat tgatagccgc aactgccatg
     4201 caaccattcc ataacatatg aaatccaaaa ttcttgatcg tccacatacc gagtggagga
     4261 gcgaaaataa aggcaacact gaacgcaaaa gaatacagag ccagatatct gccaatatta
     4321 ctttgcgtag cccgattggc aacataggtc tgcataaacg gcatggaaag tatttctgcc
     4381 agactgagca caatcattgc cacgatcaac catgcggagt gatgtaccca attgagcata
     4441 atgaaggaca ggccggtagc cagaacaccg gcaatgatga gcttatgatt ctcaatccgg
     4501 ttccctacat aatgtacaaa agccatctct gaaacaaaca caaaaagtcc gttcacagca
     4561 agcagtgttc cgatatgggc ttcaccaatc ttgtagtgtt gtgcatagta aatcggtaag
     4621 gtaaagagca tttgaaagaa taccgtggca aaaagaatga cacttataac aaaaacaagg
     4681 aaaattgtat ctttcaaagg accggtagtt tcaccacgca caggaatttc cggtcgatct
     4741 tcctcaacgg gccccttctt agtaaaatac cttctaaaaa taataaagaa agcgatagag
     4801 gaaaaaatac aggacaaacc atctccccaa aaaagcaagg tataggaaat agtggcaaaa
     4861 atacctccaa tggcaggacc tatggaatat cccagattca tcgccatcct gtttagtgaa
     4921 aatgacctgg ttctattccc ggcgtccgta aatagggtga ttgaactgtt gttggcaggc
     4981 cggaagatat cgccaatgga actttggata aaggcaagca tcagcaggtg atggtattcg
     5041 gtaatatgtg ccagaattat ccatccaatg ccacttagaa caagtgcacc gaattgtgtc
     5101 ctaaaactac catatttatc cgtcagccag ccacccgtca aagtaccaac cattgagccg
     5161 acgccataag ccgacaacac ccagcccgcc tgaatctcag aaaacccaag atgtctggta
     5221 aggtagatgc tcaggaatgg aaagaccaac gcacctgatc tgttgatcag catgatgagt
     5281 gacagcaacc aaacctctct tttaatgccg gaataggcat tgacaaagag gtgtctcacg
     5341 ttttggacga tggccatcag attgatttaa aaatgagaat aagggaacaa aaatagttag
     5401 tcctgacaat atttcagata atacttatct gatcagctta tagcatagcg caaggctcaa
     5461 cagggaggtg aacatatctt cccggacggc aaccacttta atgaacattg cacccaaagt
     5521 ctaagtcaat acagtgtatt atttcatgat cattaaattc cctttattta aacatgctca
     5581 agttattact aacaaatcca aatgcaggaa tgtagtgcgt aatatgtgaa ataagagtat
     5641 ttttataaaa atttaaacaa caattaagat taaaattcaa tgtcaatttt ccgatttaga
     5701 ctttcagcaa ttaagcaatg gcccgatatc ttcaagaatc tattcagcaa ttattttctt
     5761 gtagtgacaa ctgccctgat cctggcaggg ctatggattt atcaaattga agccggagac
     5821 aaactgtctt tttttgcatc ccataacgtt tccagaatat tattcagtct tggattatgc
     5881 atacccgcat ttttagctct tcatgtcctt gtaaataagc aaaatcaaaa aatcttatac
     5941 ttcacgttag gactgggaac aataattttg atgcttggaa tctactatgt gaattacgag
     6001 ctgagggctg ataagcaatt ctacagaaca ttggccttat ttctggcttt tcatggtctg
     6061 ctatcgctta gtgcctatag aaaatatagc tcggtcaggg atttttggca attcaacacc
     6121 aatttgttgc taagaactat tggttcactt ttctttaccc tggtgctgta tctgggagta
     6181 attgcggcaa tagcagcatt gaacaaccta ttcggggtcc atatctccta taatttgtat
     6241 tttgatctga tgattgtagc attggttgtc tacaatacat tttttgtgtt ggcaggaatg
     6301 aagacttcgt ttgatgaatc cggttcggaa tttcaatacc cgctttctct aaaagtcttc
     6361 acacaattcg ttttaatccc tttgatggca ttatatgcaa tcattctgta tgcattttcg
     6421 ataaagatta ttgtattatg gtcattgcca aagggttgga tagcctacct tgtcctttta
     6481 ttttccatcg ccggaatcct tggcttcctg ttgatatatc caatcaggaa ctcaacggaa
     6541 aaaaaatggg taaggattta ttccaaaaca ttttttatag ctcttgtccc cttgatactt
     6601 ttgttacatg tcagtatctg gacaagaatt ggccagtacg gatttaccat ccacagatac
     6661 gtggttgcca tcctggccat atggctgaca ttgataccag gatatttcct cctttctaaa
     6721 aaagacaata taatagttat tccggcatcc ctgacagcac ttttcctttt agcctgtttt
     6781 ggcccatggg gttttgatgc aatcagtcaa aaaagccagc aaaatagaat tgaaaaaatt
     6841 atgcatgacg cccaaattca ggattattat aaaaaaggta aaatcaatga atacaaagca
     6901 aatgacaagg ttactaccga gttaaactcc attattgatt accttttact aaatcatgga
     6961 gcaagatcaa tgaaaggttt tgatatcgca agttgggata aaatagccga ttcaatcatc
     7021 gaccgacaac aaaagttctt tgaagaaccg aagatggaaa aaggcgatac tgctatcagc
     7081 aattcgatca atgcatcctc attttacagt tctcccaata gctatacatt tctcaaggaa
     7141 atcaatgtac ctcaatcggg tggatataat atggccaggt ttgattatag ttattccccc
     7201 ctacaaaatc aaatagaaat catttcatat tccagattgg taccatttga tctgaatatt
     7261 tcattcaagg atcaaaataa tatcattaca ctggacactt cagggtttaa acttaatatc
     7321 aacggcaaaa gaattactgc ggatgctgaa aaaacagtca tcgatgcctt gaacaaatgc
     7381 ggaacagcat gtttcaatgc ccaatcaccc attatagtaa ccggaaagca aatggatatt
     7441 gaatatcgaa taattattaa cagcctgagc ggcaaaataa tttcagatca aaagtatgag
     7501 tttgagaatg caatgggata tctgcttttt aatcaataga actcaatctt gactgccctg
     7561 tattgcctgc catcaatcac aggtattgca aaataaatgc ccggtataag ttcatgcttt
     7621 tccacctcta taacatgccc ggatggagta tgtttgctca tcaatcttcc gtatctgtca
     7681 tagatcaata ctccatccac attccggtca aatgttattt tgattttgga ggaggaagga
     7741 ttgggaaagg cagtagctgc aatttcatct gccaaggtcc ctgtatttgc aggcgtacca
     7801 cagatgcggg gataatagat atctttagct ttgccaacaa attcgagcag gtatggccat
     7861 agattcggat cggtaaatat ctcggtatgg ccaccgcctg gcactacctc cagagtctgg
     7921 gggataccct tttcagcaca cttaggatgc aggactcttg acccataagc agtcgcaaag
     7981 ccaaagaaat cagccgaatc tattggaaca gtattatcaa tatcaccatg atagctcatc
     8041 agaaaagggt caccctcatc aaagagctct ttggtaaaag tagctcccgc ataattgaat
     8101 acacccagta tgtcatcctc ccgatgagac agattctctg tatctccggt attgcccttc
     8161 caaccaccct gggcattgat ctcgttgagg attattggcg gcacatagtc tccggagtca
     8221 agataggccg aatgcagaac agtaaccgca ccggcagaat atccgccaat catcattttc
     8281 gtggtatcaa ttctaaactg attggcgcct gaagcgtcaa ccttgaaaaa cctataggca
     8341 gccttgaagt cattcattgc tttgacaacg ccccgtataa acccggcctg atcgggaatt
     8401 ccaaacatca atcgataatc aatggaggct gtaacgaaac cgcgttttac ataatcaata
     8461 caaaatccgg ccatatccgc cttactccct gacacaaagc ctcctccatg gacaaaaagg
     8521 atcataggcc tcttctccag ctgatctcct tttggttgat atacatcgag gtataggtcg
     8581 aagttctccg gtatcggcct cgcatatttc atggaagtga tttcgtactc aggaaagaca
     8641 tattcaatat accgcttgcc atcacatcct gtggaacagg gttggctgaa ggcaaaaacc
     8701 ggaagaagac acagtaatat taatcggaaa caattcatag atattaatca attatcaggc
     8761 gtaaatgtaa atattattca actataaagg cgcttcgtta acattaattt acttagactt
     8821 gaaaatgatt atccaataaa cccccgacat caaatgacag cataacatac atgggctatt
     8881 attattaatg tcaaccttat ttaatcaaaa tcagcctaca gccggatact aacaaccttt
     8941 ccaaaataat gaaacattga tcaggatata cattaaaaat aagatcaatg gtaagtagcg
     9001 tatccatttt tcgcgcatcc ggtgtcgcct atcgaaacca taaatcttca ctattgtttc
     9061 attgtctgct cctgcaagct taaccaatcg tggtacaagc ctgttgacaa aaaaataggg
     9121 aagcggctta caacatgcca ttgcctcaaa aacaatgtcc ctgttcgttg caatatctcc
     9181 cactgaaaga aaataatcga tattggcaat agacaagagg ttgttgatgg atacaaaata
     9241 ccggccgggg tctatcttac ttttatctac gcgggcgact tcccttctca gattctgaaa
     9301 gatggtgagc atggctgttg gagtaacctg ttgctttttc gcttcctggt attttttatc
     9361 atccctgtat acgtgtgcaa gactctgttg ttgaacagag aattgcatat catagagcaa
     9421 tttatctgca ggattggaaa gacaattgta cgcttcggtt atttgcttga agatgtactc
     9481 agcgttggta ttgttcgggt ttttatccgg atggtatttg agggcaagct tgtgataagc
     9541 ttttttaact tctgtagccg atgctccggc aggcagacct aatatttggt agtaatcggt
     9601 catcctgcta catctattac attataaatc atgacaatgg taacggagaa tcctcttaat
     9661 tcaataaaat tactgccttg cttcactgtg ttacaatgtt tggaatgaag gtaaggaaaa
     9721 aatacggtag acaaaatacg gatattatgt acaattactt taaatatgta taatactgat
     9781 ttataatatt ttaaatatat cataatgata tagtaacctg ccgaggtttc caaggatcat
     9841 tacaaagaaa ccttcgcagg tccatagttc ctcagctgcc taaagctatc tgacaagctg
     9901 gaaggttgtc ctcctgttga gctgatgctc ttcttcggta cacttttcac aaggacattt
     9961 tgccagcggc atcatttcac cgaatccccg ggaggagagc ctgttgcggt tgattccatt
    10021 ggcagtcaga tagtccacga ccgatttggc ccgcctggca gataatgtct tgttgtaagc
    10081 ttcactaccc cgacaatcag tatgggaatt
//
LOCUS       DAOQQN010000065        10065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2451_length_10065_cov_5.537862, whole genome shotgun sequence.
ACCESSION   DAOQQN010000065 DAOQQN010000000
VERSION     DAOQQN010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10065
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2451_length_10065_cov_5.537862"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2533
                     /gene="metH"
                     /locus_tag="PLG24_03830"
     CDS             <1..2533
                     /gene="metH"
                     /locus_tag="PLG24_03830"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HMZ24046.1"
                     /translation="KKDYAAALEVAQQQVEGGAQVLDVNMDEGLLDSKQAMIEFLNLV
                     MSEPDIARLPIMIDSSKWEVLEAGLKCVQGRCVVNSISLKEGEAEFVQQAKLVRRYGA
                     AAVVMAFDEVGQADTIERKVSICQRAYKILVDKVGFRPEDIIFDPNIFAIATGIEEHN
                     DYAINYIEACRLIKDTCPGVKISGGVSNISFSFRGNDRVREAIHASFLYYAIKAGMDM
                     GIVNAGMMEVYEELPKDLLVLVEDVIFNRNPGATEALTAYAEQVKGESRSLEKDLEWR
                     KAPVHERLAHSLVKGITEFIIEDTEEARKMASMPLEVIEGPLMDGMNIVGDLFGSGKM
                     FLPQVVKSARVMKQSVNYLTPFIEATKSSAGDLSKGRILLATVKGDVHDIGKNIVGVV
                     LGCNNYEIIDLGVMVSADKILNEAIAQNVGIIGLSGLITPSLDEMIHVAREMQRRKFT
                     IPLLIGGATTSRTHTAVKIDGQYDGPVVHVLDASRSVAVASVLMSPDEKVKEEFIASI
                     AAEYEQVRTQRANMKSEKRMLSYSDARRNKLKIDWANYTPPMPAHPGVTLFSDFPLAE
                     IRNYIDWTPFFQSWQLTGKFPAILDDVVVGESARDLYNDAQRMIDRIIEEKWIKANGI
                     IGFWPANTVEDDLIEVYTDQSRTKILEILVCRRQQQQKAAGLPNISLADFIAPKESGV
                     IDYIGAFAVTGGMNIEEKLHEFEASQDVYNAILLKAIADRFAEAFAECMHHLVRTKYW
                     AYAPDENLTNEDYIRERYRGIRPAPGYPACPEHSEKGKLFRMMDVTANTGIELTEFFA
                     MVPTAAVSGWYFSHPDSKYFGVGDIPDDQKAAWKAFEFHRERVGD"
     gene            complement(2713..3477)
                     /locus_tag="PLG24_03835"
     CDS             complement(2713..3477)
                     /locus_tag="PLG24_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMZ24047.1"
                     /translation="MRTYKILLTLLALVMIIGCKQAPPSNESEAVNYYLTAPEFAEKL
                     KEMPDVQLIDVRTPMEYAGGHIQHAVNINIDGANFQSDISKLDKSKPVMVYCLSGARS
                     AAATRQMRADGFSQVYDMAGGMLKWRAAKLPETTEVPGQVQTPAPTSGTAMASAKNLT
                     KEQFEEMTRSSKTVLVDFYADWCAPCKKMEPYLKEIATDMSDKVHLVRINVDENQQLA
                     MELGIDAIPVLRVYKKNALSWNNMGYVDKEEVLKQL"
     gene            complement(3481..3774)
                     /gene="trxA"
                     /locus_tag="PLG24_03840"
     CDS             complement(3481..3774)
                     /gene="trxA"
                     /locus_tag="PLG24_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HMZ24048.1"
                     /translation="MTFNEIIAQDKPVLVDFFAEWCGPCKMMKPILEDLKQKVGDDVT
                     IIKVDVDKNPGAAQQYRIQGVPTLMLFKKKEIKWRQSGVVSAANLEQVIKQYQ"
     gene            complement(3779..4354)
                     /locus_tag="PLG24_03845"
     CDS             complement(3779..4354)
                     /locus_tag="PLG24_03845"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HMZ24049.1"
                     /translation="MQDKKLYTALLLALIYLSCVNTPTPVKNTDPKEFVALGDSISTQ
                     VQAVLLKNVSEALGKGGPVYAIDYCNVNAIPLTDSMSKAYHVEIQRLSDKNRNPVNAI
                     ISDSDQKAWSKIQSDKETFAELNETGEVLYYKPIFTGMPACMKCHGSEQDIASDALQL
                     LGEKYPEDKATGYRMGDLRGMWKIKFNKTTN"
     gene            complement(4354..4668)
                     /locus_tag="PLG24_03850"
     CDS             complement(4354..4668)
                     /locus_tag="PLG24_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMZ24050.1"
                     /translation="MLALLKNLFGLGPKTDYKELVKNGAQIIDVRTKSEFQSGHIKGS
                     VNIPLQMLSASLAKIKKDKPVITCCASGMRSSSAKSILKSNGYVEVYNGGGWSSLQTK
                     LR"
     gene            complement(4676..4942)
                     /locus_tag="PLG24_03855"
     CDS             complement(4676..4942)
                     /locus_tag="PLG24_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMZ24051.1"
                     /translation="MKDKMTIIDVRTPMEFTGGNVSGSINIPLDEIETRVEEIKQMQQ
                     PIVLCCASGNRSGYATSYLKSLGIECENGGSWFEVNFLVNNLNN"
     gene            complement(4917..5156)
                     /locus_tag="PLG24_03860"
     CDS             complement(4917..5156)
                     /locus_tag="PLG24_03860"
                     /inference="COORDINATES: protein motif:HMM:NF039355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HMZ24052.1"
                     /translation="MIPFILKYKMMLIGILIGAVAGYAYYYFVGCAGGTCPISSKPLN
                     STLYGALAGAVFFSDTKSNKSNKLNTKNERQDDNH"
     gene            complement(5156..5596)
                     /locus_tag="PLG24_03865"
     CDS             complement(5156..5596)
                     /locus_tag="PLG24_03865"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HMZ24053.1"
                     /translation="MKHEVEAEWKGKMRFDSRIGDHIVAMDVPVHSGGDNSGPSPKPF
                     ILASLAGCTGLDVVAILTKQGRKPESFRLKVSGELSKKRPVEYVAAHVLYEISGNADD
                     MEAAIDAVTASQEELCGVGHMLRHFMPITWQIIYNGNEIFNNKA"
     gene            complement(5660..6289)
                     /locus_tag="PLG24_03870"
     CDS             complement(5660..6289)
                     /locus_tag="PLG24_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ24054.1"
                     /translation="MTIRDRFKNIFEPELLSQIESRSEIKLIPEGGIILDIGQSVRIV
                     PLLLKGVLKISRVDDSGREILLYYVHANESCAMTFTCCMQQHPSEIKATAESEVELLA
                     IPIGVMDEWLSKYPTWKSFVMRTIRNRFNELLGTIDQLAFQKLDERLVTYLKEKSRAT
                     GSTLINLSHEDIAGELASSREVISRLLKKLENDNKVLLFRNQIKILRDL"
     gene            complement(6718..6957)
                     /locus_tag="PLG24_03875"
     CDS             complement(6718..6957)
                     /locus_tag="PLG24_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24055.1"
                     /translation="MEKKIKLLWDFRGPDAKQIAEHYDHHLQEFIEANKLKNDITGVE
                     NISDMYSIAFMVIDEDEFEMVRNVLKPKRGTVYEG"
     gene            7551..>10065
                     /locus_tag="PLG24_03880"
     CDS             7551..>10065
                     /locus_tag="PLG24_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HMZ24056.1"
                     /translation="MKFLLKNEKMREGARMYNIIWFAILALFAIENNHSGLFGLGSDK
                     PAKASAVNKSSLPPESLNCIDSTNISISENGVSTLNPGYFVPPTYPNFAFLQLSIVGR
                     PAPIINCDDVGHYLTAVVHDPIANVTCSSVFKVEDKLPPVFNCVTLELPCGIDVYSTP
                     PPYTPLVSAVDNCTNPPTIEILTHTEKIFNCPSPYTMEIVRTYKATDASGNSSTCQDT
                     VRFLRPILSQIVFPDDTCFNCDAFTGDTAVTGQPTWRGYPLTGVCATWFGYSDQIIDM
                     GCPGRYKIRRTWNVMNECNGTSINHVQNIMSTDTTAPVITCPNDSILQTNFGSCVASY
                     TFPSPQATDNCSTVITFQYKVDGVIVNAPTTTLSLGSHSIEVIANDGCYNTSSCTYNV
                     TVEDKQGPSITCHDIIVSLNESGIGVVHKDSLDFDYFDNCSGPLTVKIKKSTSSTWSD
                     TVNYTCAELGGNNILQFRVCDQYGNCTICEDSVTVQDKMAPMVDCGTTPVELNCTDFA
                     DFDPADYYPVATDNCGLVTFTNTISNGMNCNFVGDYTVTYTAMDASGNQASCSRIFHF
                     NNTNPLDLSDITWPADVNYTGCNPDLVGDSISGSVILNGTFCNPIYIYSSLTDTLVNG
                     SGCAVVSKEYVVIDSCVYNLSGGTQGKFTHTQFITSTGIIPPTISVPADVTVVPDDFI
                     NCTANVSLDPATASGCSTPITITNSFNGGGADASGDYPYGTTTVVYTATDVCGLTAQG
                     STDVTVEFATNDMISCPGDSTITCLNLFDSTYLPSPTIFNTCAGYTLDTLITGTIYEC
                     DNSELTVTYTITDTHGRSDACSFNITIQGPTPLTENDITW"
BASE COUNT         2664 a         2129 c         2249 g         3023 t
ORIGIN      
        1 gaaaaaagat tatgcagcgg ctctggaagt agctcaacag caggtggagg gcggggcaca
       61 ggtcttggat gtcaatatgg atgaaggttt gctggatagc aaacaggcca tgattgaatt
      121 tctcaatctc gtcatgtcgg agccggatat cgccaggttg ccgatcatga tcgattcctc
      181 caaatgggaa gtcctggagg ccggcttgaa gtgtgtccag ggtcggtgtg tggtcaattc
      241 gatctccctc aaggaaggag aggcggaatt tgtccaacag gcaaaacttg ttagaagata
      301 tggtgctgca gctgttgtaa tggcttttga cgaagttggt caggctgata ccatagaaag
      361 aaaggtttca atatgtcagc gtgcgtacaa gattttagta gacaaagtcg gatttaggcc
      421 tgaagacatt attttcgacc cgaatatatt tgccattgcc accggaatag aggaacacaa
      481 cgattatgcg attaattaca tagaggcgtg tcgcctgatc aaggatacat gtccgggtgt
      541 aaagatcagc ggtggtgtaa gcaatatttc attttccttc cggggtaatg accgggttcg
      601 ggaagcgata cacgcttcct tcctctatta tgctatcaag gccgggatgg atatgggtat
      661 agtcaatgca ggtatgatgg aggtatatga agagttgcca aaagacttac tggtattggt
      721 tgaggatgtg attttcaatc ggaatccggg tgctaccgag gctttgaccg cttatgccga
      781 acaggtcaaa ggagaatcac gttctttgga gaaagacctt gaatggcgta aggcccctgt
      841 gcacgaaaga cttgctcaca gccttgtcaa ggggattaca gaatttatca ttgaagatac
      901 cgaggaggcg cgtaaaatgg cttcaatgcc tttggaggtc attgaagggc cccttatgga
      961 cggaatgaat atcgtcggcg acctttttgg ttccggaaaa atgttccttc ctcaagtggt
     1021 aaaaagtgcc cgtgtcatga agcaatccgt caattatctg acacctttta ttgaagctac
     1081 caagtcctct gcaggcgatc tttcaaaggg aagaatattg ctggcgacag tcaagggaga
     1141 tgtccatgac attggaaaaa atatcgtcgg tgtcgtgctg ggatgtaata actatgaaat
     1201 tatcgatctg ggtgtcatgg tgtctgctga taagatactt aatgaagcaa ttgcgcagaa
     1261 tgttggaata atcgggctga gcggtctgat taccccttcc ctcgatgaga tgatccacgt
     1321 tgcaagggaa atgcaacgtc gtaaatttac cataccattg ttgattggtg gtgcgacgac
     1381 ttcccgtact catactgctg taaagattga cgggcaatat gatgggcctg tggtacacgt
     1441 gctggatgcc agccgtagtg ttgctgtagc ttctgtactg atgtcgccgg acgaaaaagt
     1501 aaaagaagaa ttcattgcat ccattgctgc agaatatgag caggttcgca cacaacgggc
     1561 gaatatgaag agtgaaaagc ggatgctttc ttatagtgat gcccgcagga ataaattaaa
     1621 gattgactgg gcaaattata caccacctat gccggcacat ccgggagtaa ccttgtttag
     1681 tgattttcca ctggctgaaa tcagaaatta tattgactgg acgccctttt tccagtcctg
     1741 gcaattgacc ggtaaatttc cggcgatatt ggacgatgtg gtcgtaggtg aatcggcccg
     1801 tgacttatat aacgatgccc agcgtatgat cgaccggatt atcgaggaaa agtggatcaa
     1861 ggccaatggc atcatcggct tttggccggc taataccgtt gaagacgatt tgatcgaagt
     1921 atatacagat caatcaagaa caaaaatctt agaaatcctt gtatgcagga gacaacagca
     1981 acaaaaggct gccggcctgc ctaatatttc actggcggac ttcatcgctc caaaggaaag
     2041 tggtgtgata gattacatcg gtgcatttgc ggtcaccggc ggaatgaata ttgaagagaa
     2101 attacatgaa ttcgaagctt ctcaggacgt atataatgct attcttttaa aggcaatagc
     2161 agacagattc gccgaggcat tcgcagagtg tatgcatcac ctggtcagga ccaaatactg
     2221 ggcttatgca ccggacgaaa acctcaccaa tgaggactac attcgcgaga gatatcgcgg
     2281 tattcgtcct gcacccggct atcctgcatg cccggaacac tccgagaaag gcaaactgtt
     2341 caggatgatg gacgtcactg caaatacagg tattgagtta accgaatttt ttgccatggt
     2401 gcccacagct gcagtgtcag gctggtattt cagccatccg gacagcaaat atttcggcgt
     2461 aggagatatt cccgatgatc agaaggctgc ctggaaggca tttgaattcc atcgtgagag
     2521 ggtcggggat tgatttggcc caattttcag gataaggggg attcgggtaa gcgagctttg
     2581 ttttctttta gtacaatact ttcaatcata aattatcatg cctaagagaa tcgattcaat
     2641 gcaaaaaatt gggccggtat tcccattgga cgaccgaccc ggttttatta ctgctctatg
     2701 gttttgactt acttataact gcttcagtac ttcttcctta tcaacataac ccatgttgtt
     2761 ccaggataaa gcatttttct tatacacccg gagcacaggg atggcatcga tacccagttc
     2821 cattgccagt tgctgatttt catcgacgtt gatgcgtacc aggtgaactt tgtcggacat
     2881 atcagtggca atttccttca gatatggttc catcttttta cagggagcac accagtcggc
     2941 ataaaagtca accagtaccg tcttgctgga gcgtgtcatc tcttcaaact gctcctttgt
     3001 caggtttttg gcagaagcca tcgcagtgcc tgaagtaggg gcaggtgtct gtacctgacc
     3061 ggggacttcc gttgtttccg gcaattttgc agctctccat ttgagcatgc cgcctgccat
     3121 atcgtatacc tgagagaatc cgtcggcacg catttgacga gtagcggcag cacttcttgc
     3181 accactcaga cagtacacca tcacaggctt ggatttgtcc aatttagata tatcggattg
     3241 gaaattagcg ccatcaatat tgatattcac tgcatgctgt atgtgacctc cggcatattc
     3301 catcggcgta cggacatcaa tcagttgtac atcgggcatt tccttaagct tttctgcaaa
     3361 ttcaggggct gtaaggtaat agtttactgc ttccgactca ttagagggag gtgcttgctt
     3421 acatccaatt atcattacta aggccagcaa ggtcaacaat atcttataag tcctcatagt
     3481 ttattgatat tgtttgatga cttgttcaag atttgcagcg gagacaactc cagactggcg
     3541 ccatttaatt tccttctttt tgaaaagcat cagggtaggc acaccctgaa tcctgtattg
     3601 ttgtgcagca ccgggattct tgtccacgtc caccttgatg atggtaacat catcaccgac
     3661 cttttgcttc aagtcttcca ggataggctt catcatttta caagggccgc accattctgc
     3721 gaagaagtca accaggacgg gtttgtcctg tgcaatgatt tcattaaaag tcatattgtt
     3781 aatttgttgt tttgttaaat tttattttcc acatacctct cagatcgccc atccggtagc
     3841 cggtagcttt gtcttcaggg tatttttctc cgagcaattg aagggcatca gatgcaatat
     3901 cctgctcgct cccatgacat ttcatacaag caggcattcc ggtaaatatg ggcttgtaat
     3961 acaacacttc ccctgtctca ttaagttccg caaatgtctc cttgtcggat tgaatctttg
     4021 accaagcctt ttggtctgaa tccgaaataa tggcattgac aggattccgg tttttatcac
     4081 tcaatctttg gatttccacg tgataggcct tcgacatgga gtcggtcagt ggtatcgcat
     4141 ttacattgca gtaatctata gcatacacag gccccccttt tccgagggct tcactgacat
     4201 ttttaagcaa cactgcctga acctgagtag agatactgtc tcccagggcc acgaattcct
     4261 tagggtcggt gttctttaca ggagtcggtg tgttgacaca actgagatag atcagtgcaa
     4321 gcaataaggc tgtatataac ttcttatcct gcattatctc agttttgtct ggagagaact
     4381 ccaacctcca ccattataga cttcgacata accgttagat ttgagaatgc tctttgcgga
     4441 ggagcttctc atacctgatg cacagcatgt gattaccggt ttgtccttct ttatcttagc
     4501 cagactggct gacaacatct gtaatggaat attgacggag cctttgatgt gaccggattg
     4561 aaattccgat ttagtgcgta catcgatgat ttgagcaccg ttcttgacca attccttgta
     4621 atctgtcttc ggtcctaagc cgaacaggtt ctttaacaac gccagcattt ttttatcaat
     4681 tattaaggtt gtttactaaa aaatttactt caaaccagct ccctccgttt tcacattcga
     4741 tacccaagga cttgaggtat gatgtggcat atccacttcg attaccactg gcacaacata
     4801 aaacaatggg ttgttgcatt tgtttgattt cttcgaccct tgtttcgatt tcatccaggg
     4861 gtatgttgat gcttccgctt acattcccgc ctgtgaattc cattggtgtt cggacatcaa
     4921 tgattgtcat cttgtctttc attttttgta ttcagtttat ttgatttgtt tgattttgtg
     4981 tctgaaaaga ataccgctcc tgccaatgct ccataaaggg tactgttgag cggctttgat
     5041 gagattggac aggttcctcc ggcacaacct acgaaataat aataggcgta tcctgctacc
     5101 gctccaatca ggatgccgat cagcatcatc ttatatttaa gtataaaggg tatcatcagg
     5161 ccttgttgtt aaatatttcg ttcccattgt aaataatctg ccatgtaatt ggcataaagt
     5221 gtctgagcat gtgacctacg ccacacagtt cttcttgaga ggctgtgacg gcatcgatcg
     5281 ccgcctccat atcgtcggca ttgccggata tttcgtacaa tacatgggca gcaacatatt
     5341 ctaccggacg ttttttgctc aattcaccgg agactttcaa cctgaatgat tccggcttac
     5401 gtccctgctt tgtaaggatg gccacaacat caagcccggt gcagccggca agcgatgcca
     5461 gtatgaaagg ttttggagat ggtccgctat tatcaccccc cgaatggaca ggaacgtcca
     5521 ttgctacaat atgatcacca atccggctgt caaatcgcat cttgcccttc cattctgctt
     5581 ctacttcgtg tttcatgtgg atatctcttt tttgcgatgc aaaggtctga agtagaacat
     5641 tccttttgcg tgacaaatgt cacaaatcgc ggagtatctt gatttggtta cggaagagaa
     5701 gtactttgtt gtcattttcc agttttttca gcaaacgtga gatgacttcc cgggaagaag
     5761 cgagttcgcc tgcaatatcc tcatgggaaa ggttgatcag cgtggaaccg gtggctcttg
     5821 atttttcttt gagataggta accagacgtt cgtccagttt ctgaaaagct aattggtcga
     5881 tggtgccgag cagttcgttg aaccgatttc ggatggtacg catcacaaaa cttttccagg
     5941 tgggatattt ggagagccat tcgtccataa caccgatggg gatggcgagc agctccacct
     6001 cactctcagc ggttgccttg atttcgctcg ggtgttgttg catacagcag gtgaaggtca
     6061 tggcacaact ttcattggca tggacgtagt ataggagaat ctcacggccg gaatcatcca
     6121 cccggcttat tttaaggacg cctttgagca aaagaggtac gattcgtacc gattggccga
     6181 tgtcgaggat gatgccacct tccgggatca gcttgatctc tgagcgggat tcgatttggg
     6241 acagcaattc cggttcgaaa atatttttga atcgatctct gatagtcatt tgcgatattt
     6301 aattttttgt caatttatga ttaatgtatg tactttgttc ttttgagggc atactcctgt
     6361 tatcatgtta aaaccttgcc tcttcttctc ctcaattttc aatgatataa taaagatggc
     6421 cacaatgatg acccttgttt atttccgtat ttctacgacc acatttgtca ggtttgattc
     6481 tttttttcat ttgtcaataa taattgcatt aaatatagaa ttattatacg aatgtagcgc
     6541 gcggtagctc tgcgcagaaa gtgtttttca aagcccctaa tcccggagag ataatagtcg
     6601 cataatgtta ttgatcggtc gtctcatagg ataataatcg attatttatt gcctttaatc
     6661 tgacaatcaa tttcggacca atcattctat attccaatag ccgctgacaa ttgatattta
     6721 tccctcgtac actgtcccac gcttgggctt caacacattg cgtaccatct caaattcatc
     6781 ctcatcgatg accatgaaag caatcgaata catatccgat atgttttcca ccccggtaat
     6841 atcatttttc agtttgttgg cctcgataaa ttcctgaagg tgatggtcgt aatgttctgc
     6901 tatttgtttg gcatcaggac ccctaaagtc ccaaagcagc tttattttct tttccatgaa
     6961 tattttaagt taaaactcaa aaatagagtt tttggttcga aactgacgac tcaataggat
     7021 tggctattga gttagttcgg ttattatccg tgcctttttt ctttcagctt taggaaagga
     7081 gcattaaaaa tattgtaggt ttcttgacgt ggcttttatc ttgtttgtga taagtaaaag
     7141 attatcaatg tgatagatgt ggtgtttttc agttttatat tgaatttaaa ctaatatatt
     7201 acaaaaaaat ataatatata atccttcggc ggtattgccg ttgaaaacat attacataat
     7261 tttgtaatat ctaaatgatg ataaaaaaga ataatatgat ttattaaaat atttaatata
     7321 tcattaaaaa tataacttaa tagaaccgaa aggccggtct tttgcggggc cggccgcctt
     7381 ttaagtaaat tctctttccc aaacacagta ttcaaatccg gtgaatccgg tatgatatat
     7441 tttgtaataa ggtttcttgt ccaaggggtg gtaaaagtat catcctttgg tctttttaat
     7501 ctgtacaagc tatatccctt ttttcaaaat agaaactaca actatcggtt atgaaatttt
     7561 tacttaaaaa tgaaaaaatg agggagggag caaggatgta taatattata tggtttgcaa
     7621 tccttgcctt gttcgcaatt gaaaacaatc attcaggtct tttcgggtta ggctccgata
     7681 aaccggcaaa agcatctgca gtgaataaga gtagcttgcc acctgaatcc ctcaactgca
     7741 tagacagcac gaatatttcc atcagtgaga atggcgtgtc tacacttaat ccgggatatt
     7801 ttgtgccacc tacctatcca aattttgcct ttttgcaact gagtattgta ggacgtccgg
     7861 ctccaatcat caattgcgat gatgtcggtc attatcttac tgcggtagtt catgatccaa
     7921 tagccaatgt aacatgttct tccgtgttta aggttgaaga caagttgcca cctgtcttta
     7981 attgtgtgac actggaatta ccatgtggta ttgatgtgta ttcgacacct ccgccatata
     8041 cgcctctggt aagtgctgtg gacaattgta ccaatccgcc caccatcgaa attttgactc
     8101 atacagaaaa gatcttcaac tgtccatcac cctatacgat ggaaatcgtc agaacttata
     8161 aggcaaccga tgcatccggc aattcatcta cctgtcagga tactgttcgt tttttaagac
     8221 caattttgag tcaaatagtt tttccggatg acacgtgctt caactgcgat gcattcacag
     8281 gagatactgc tgttaccggc cagcctacat ggcgcggata tccgcttaca ggggtatgtg
     8341 cgacctggtt tggctattct gatcagatca tagacatggg ctgtccggga aggtataaga
     8401 taaggcgtac gtggaatgtc atgaatgaat gtaatgggac atccatcaat catgttcaga
     8461 atatcatgtc caccgataca acggctcctg ttatcacctg cccgaatgac tccatccttc
     8521 agacaaattt tggtagctgt gtggcaagtt ataccttccc aagtcctcag gcaacggaca
     8581 attgctctac agtgattaca ttccaatata aggtcgatgg ggtgatcgtc aacgcaccga
     8641 ctaccacact ctcgctgggt agtcattcga ttgaagtcat tgccaatgac ggttgttata
     8701 atacttcttc ctgtacgtac aatgttaccg tggaagacaa acaaggtcct tcaattacat
     8761 gtcacgatat cattgtttca ctgaatgaat cgggtattgg ggtagttcat aaggattctc
     8821 tcgacttcga ctatttcgat aactgctcag gtccattgac cgtaaagata aagaagtcca
     8881 cttcatccac ctggtcggat acggtcaact atacttgtgc cgaacttgga ggaaacaata
     8941 ttctgcaatt cagggtgtgt gatcagtatg gcaattgcac catatgtgag gattcggtta
     9001 ctgtgcagga taaaatggcg ccgatggttg attgtggaac tacgccggtt gaattgaatt
     9061 gcaccgactt tgcggatttt gacccggctg actattatcc tgtcgcaaca gacaactgtg
     9121 gattggtgac atttaccaat acaatttcca acggtatgaa ttgtaatttt gtgggtgact
     9181 ataccgtgac ttatacggca atggatgctt ccggtaatca ggcttcatgc agcaggattt
     9241 tccactttaa caataccaat ccgctggatc tatctgatat tacctggccg gcagatgtca
     9301 attataccgg atgtaatccg gatctggtcg gcgattctat atcgggttct gtcatattga
     9361 atggtacttt ctgtaatccg atttatatct attcttccct gacagatact ctggtcaatg
     9421 ggtctggttg tgcggtagtg tcaaaggaat atgtggttat tgactcatgt gtatataacc
     9481 tttccggagg tacacagggg aagtttacac atacgcaatt tattacatcg acaggcatta
     9541 ttcctccaac aataagtgtg ccggcagatg tgactgtggt gcctgatgat tttatcaatt
     9601 gcactgcaaa tgtcagtctt gatcctgcaa cagcgtccgg atgtagtacg cccatcacga
     9661 ttaccaacag cttcaatgga ggcggagcgg atgcaagtgg agattatcct tatggtacga
     9721 cgaccgttgt ctatactgct actgatgtat gtggtctgac agctcaggga tcgaccgatg
     9781 taacggttga atttgctaca aatgatatga tcagctgtcc cggagactca acgattacat
     9841 gtttgaatct attcgactct acttatctgc catccccaac aattttcaac acttgtgccg
     9901 gatatacatt ggatactttg ataaccggta ccatatatga atgcgataat tctgaattga
     9961 cggtcactta caccatcacg gatacgcatg ggagatctga tgcatgcagc ttcaatataa
    10021 cgattcaggg accgactccg ttgacagaaa atgatattac ctggc
//
LOCUS       DAOQQN010000066         9874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2539_length_9874_cov_5.820858, whole genome shotgun sequence.
ACCESSION   DAOQQN010000066 DAOQQN010000000
VERSION     DAOQQN010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9874
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2539_length_9874_cov_5.820858"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2092)
                     /locus_tag="PLG24_03885"
     CDS             complement(<1..2092)
                     /locus_tag="PLG24_03885"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HMZ24057.1"
                     /translation="MGKTVIKKEKTTYTQDEAIEASLKYFGGDDLAARVWVNKYALKD
                     SHGNLYEKTPDDMHRRIASEIARVEKNYPNPLSEEEVFELIRNFKYIIPQGSPMTGIG
                     NPYQIASLSNCFVIGHDGPSDSYGGIMKIDQEQVQLMKRRGGVGHDLSHIRPKGSPVK
                     NSALTSTGIVPFMERFSNSTREVAQDGRRGALMLSVSIKHPDSESFIDAKLEEGKVTG
                     ANVSVRIDDEFMRAVQDQSAYQQQYPLMSTDPMYVKKIDAAALWKKIVHNAWRSAEPG
                     ILFWDTIIRESVPDCYADLGYKTVSTNPCGEIPLCPYDSCRLLAINLFSYVENPFTGE
                     ARFNWDLFKQHIHFAQRIMDDIIDLELEKIDEILRKVDEDPESDQIKSIERNLWLSIR
                     EKAHQGRRTGIGITAEGDMLAALGYRYGSPEGIDFSVEVHKTVALEAYRASVHAAKER
                     GAFGIYDAEREKNNPFIQRIKEADAQLYYEMLEYGRRNIAMLTIAPTGTTSLMSQTTS
                     GIEPVFLPVYKRRRKVNPNDKNVRVDFVDEVGDSWEEYAVFHHRFRQWMEVNGHDTKK
                     HYTHEELDNLVKASPYYKATSNDVDYLNKVRMQGAIQKWVDHSISVTINMPNDVSEET
                     VGQCYMEAWKAGCKGVTVYRDGSRSGVLVAAKDNKNAEAAADEPAPFPTTRPQVLDAE
                     IVRFQNNREKWIAFI"
     gene            2421..3533
                     /gene="mutY"
                     /locus_tag="PLG24_03890"
     CDS             2421..3533
                     /gene="mutY"
                     /locus_tag="PLG24_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HMZ24058.1"
                     /translation="MHNFSLSKNSYIDFRNKINAWYHPDFRKMPWKGEKDPYKIWISE
                     VILQQTRVQQGWEYYVNFLEKFPTLQVLASSDIDDVLKTWQGLGYYSRARNLHLGARR
                     VVEHFGGRLPANYHDLLKIPGIGPYTAAAIASFAFGLPYAVVDGNVVRVLARYFNIEA
                     AFDSTAGRKIFSIIAGNVLEPADPGRFNQAMMDLGATICTPTATRCGICPLRDGCMAF
                     KLDRIKQLPIKPKKIIRRERFFNYLIIRSTDSCLLFRRQKGDIYSGMYEFLLWENEKE
                     ASTMDMGQWLQDIGINGRAEPILKLPPQILTHQKIIATFYIIHVADLPTLESAIEVAF
                     NDLPQFAFPKFIQTAIDRIDLKKIEGVIQPQISHNS"
     gene            3979..4515
                     /locus_tag="PLG24_03895"
     CDS             3979..4515
                     /locus_tag="PLG24_03895"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ24059.1"
                     /translation="MKKILLLCTFSFLTISISFSQKGSSFIIANIGVDNYKIVNNEYT
                     KTFKVGGGYGYSITEPMALGAEFSYSTFKIKNALTTSSSTSWSAGPFIRSTFPLSDMI
                     SFYSQLGAGYQTEGNKTASGFYAYLNPAAMLKVDKVTYITFTTGLIRYDNIKSFYSRF
                     RFLYGNPTLGVMFKINGK"
     gene            complement(4586..5920)
                     /locus_tag="PLG24_03900"
     CDS             complement(4586..5920)
                     /locus_tag="PLG24_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ24060.1"
                     /translation="MKVKLFVLIGLITSIQYSQGQLPSIPATLSLEEARQLAIKNNPT
                     ILNSNLNIAISQKQVEEARWKKIPQVYTNYDIRSNLIIPTTPVPARAFDPAASENVLL
                     PLKFASRWSSNIGLNAHYDLFNPQTRGAVREAEHQTLISTVEAAISASDLQFAINLDY
                     VACIIARKQVVMAALDTAIMTRILSVTSDRYKAGRSTDADINNAMANKNRSVSDYFRA
                     ENILAQAGARLLTDLGLDPAQSEMPILTDSIPVLMTLFVKDVHNAPEPLHLQRIAHQQ
                     TLSEIRLQNTRDGFLPTVSLNGFLGANYYDNGFNIFNESHWYGNSYLGLSVTIPITQG
                     LDRIKKADVLKLQILADRNRYLTEQNQHNFEVSQARNDYLFRKRDLDLMEQSMELASK
                     NFKLSLDQFKEGRVMVSELSTSELHYRQAKAEYLQAAYDLIVAGMRLEKAIR"
     gene            complement(5936..7072)
                     /locus_tag="PLG24_03905"
     CDS             complement(5936..7072)
                     /locus_tag="PLG24_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ24061.1"
                     /translation="MFRTAIKFIWFDRPKSIGIIVGIVISSFLIGQQLGTLHFITNVM
                     GALIDHANHNTGEIWVVDNTTANVNNLFRLDSRLVNEIRSIPGVQHTYPIVVTSVSVT
                     LPNGRTTPFTIIGSEAPSFAGGPDTARITEGDLSALIRANSVSAEYFDSRSLKIPLNI
                     GPRLEINGKSAIIRLKTRHIQAFGGHFLYSNIDNARFYGNYPNDKVSIISVKIKPGVN
                     VRKVASDINQTFYGVKAWELQDLRQSTVRELLSSTNMGVSFGTLVVFAIITGFFIIGL
                     TLYSAVLDRLRDYGTYKAIGATNGYVRRLILAQAILFALTGYVIALTLLFFFKQGMAK
                     AGLIVQITPSLAGFLLFATLFISVGGSLFAISKINKLEPASVFK"
     gene            complement(7075..8037)
                     /locus_tag="PLG24_03910"
     CDS             complement(7075..8037)
                     /locus_tag="PLG24_03910"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HMZ24062.1"
                     /translation="MIDSMKNFLALTILFLITSCSNKQPSRQATIASDTLSVPHNIEI
                     IVGIGKVEPLDGVIQLASSTGGIVNSVFRQEGDTVKAGEAIFTLQPENASLKIRKIKA
                     QIATERQQIVANQTAIGQYEAQLNYKTANLEAGRKLAQSGAETLQNIANQASEIDVLK
                     NQIEQGRQAVVISRSRMDELQADLRLAEHDLGSLTIRSPSAGILLSQNAQPGSAIQPL
                     QAYAYLAPRGPLVVHGEVDELFADRLALGQEATLHLMGDKQIVGSGKIIFLSPQLNNK
                     SLFTDMPGEAQDRRVRRFKIRIDPSDRPMLLNTKVECEIHLKDH"
     gene            complement(8048..8740)
                     /locus_tag="PLG24_03915"
     CDS             complement(8048..8740)
                     /locus_tag="PLG24_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ24063.1"
                     /translation="MEIVASIRRASKKYKMGDTDITALTETTLEFHSHELTLILGPSG
                     SGKTTLLSLLGCVIYPTTGDVIVMNQNIGTLKEKKLSRIRLNNIGFVFQGFNLIQPLT
                     ALENVMMPLILQGENRRQAKARAMIALEKVDMISKMNNLPHTLSGGQRQRTAIARALV
                     TNPAILLCDEPTASIDSKNASKVMEELKTLSMQGKSVIVVTHDTRLIKYADRIIYLSE
                     GRTSETPFPEEQ"
     gene            9314..9658
                     /locus_tag="PLG24_03920"
     CDS             9314..9658
                     /locus_tag="PLG24_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HMZ24064.1"
                     /translation="MPDSLKPCPQCNYPYAYASEGGLFSCPECGHEWNPEEIYSLEDV
                     LSVFDANGNELKNGDTVVIIKDLPVKGAPKPIKAGTKVKNIRLSEGDHNISCKIDGFG
                     AMGLKSEFVRKA"
BASE COUNT         2651 a         2039 c         2191 g         2993 t
ORIGIN      
        1 ctatgaaggc gatccatttc tcccgattat tttgaaatct gacaatttct gcatccagta
       61 cctgtgggcg tgttgtaggg aatggtgctg gttcgtcagc ggctgcttca gcatttttat
      121 tatcttttgc ggcaaccagg acaccagacc ttgagccgtc acgatagaca gttactcctt
      181 tacaacctgc cttccaagcc tccatataac actggccgac tgtttcctcg cttacatcat
      241 tgggcatatt gatggtcacc gagatggaat gatccaccca tttttggatt gcaccctgca
      301 ttcttacctt atttaggtag tcaacatcat tagaggttgc cttgtagtaa ggagatgcct
      361 ttacgagatt atccaattct tcatgagtat aatgtttttt tgtgtcgtgg ccattgacct
      421 ccatccactg tcggaatctg tgatggaata ctgcatattc ttcccaggag tcgcctacct
      481 catcaacaaa atccactctg acattcttgt cattggggtt gaccttgcgg cggcgtttgt
      541 acacaggaag gaatacaggt tcgatacctg atgttgtctg agacattaag ctggtggttc
      601 cggtaggggc aatggtaagc atggcgatat ttctccttcc atactcaagc atctcgtagt
      661 acaattgggc atccgcttct ttgattcgct ggataaacgg attgttcttt tctcgttcgg
      721 cgtcatagat accgaatgca cccctttcct ttgctgcgtg aacggacgcg cgataggctt
      781 caagggccac agttttatgc acttcaactg aaaaatcgat gccttcagga cttccgtaac
      841 gatagcccaa agccgccagc atgtcaccct ctgctgtaat gccgattcct gtacgtcttc
      901 cctggtgtgc tttttcgcgg atggataacc aaagattcct ttcgatgctc ttgatctggt
      961 cgctttccgg atcttcgtct acttttctca gtatctcatc aatcttttcc agctcgaggt
     1021 cgatgatatc gtccatgatt ctctgtgcga aatggatatg ttgtttgaat aagtcccaat
     1081 tgaatcttgc ctcaccggta aaaggatttt caacatatga aaacagattg atggccagta
     1141 gtcgacagga atcgtagggg caaagcggaa tctcgccaca aggattggtc gaaaccgtct
     1201 tgtatcccag atcggcatag caatcaggta cggattcccg gatgatagtg tcccagaaaa
     1261 ggataccggg ctcggcagag cgccatgcat tgtgcacgat tttcttccaa agagccgctg
     1321 cgtcaatctt cttgacgtac atagggtctg tactcatgag cgggtactgc tgctgatagg
     1381 ccgattgatc ctgcaccgct cgcatgaatt catcgtcaat tctcactgat acattggctc
     1441 ccgtcacttt gccctcttcc agcttggcat caataaaact ttcagagtca gggtgcttga
     1501 tgctgactga caacatcaat gcgcctctgc ggccatcctg tgcgacttcc cgtgtagaat
     1561 tggaaaaccg ctccataaag ggcacgatac ctgtggaggt caatgcggaa ttcttgaccg
     1621 gagacccttt aggtctaata tgtgacaggt cgtgacctac accgcctctt cgtttcatca
     1681 actgcacctg ttcctgatcg atcttcatga ttccgccata agagtcagat gggccatcat
     1741 ggccaattac aaagcaattg gacaatgaag caatctgata cgggttgcct attcctgtca
     1801 tcggacttcc ctgagggatg atatatttga aatttctgat cagttcaaaa acttcttcct
     1861 ctgaaagggg gttgggataa ttcttttcta cacgtgcaat ttcggaagcg atcctgcgat
     1921 gcatatcgtc cggtgtcttt tcataaagat tgccatgaga atccttcaac gcgtatttat
     1981 tgacccaaac cctcgcagca aggtcatctc cgccaaagta ttttaggctg gcctcaatag
     2041 cctcatcttg tgtatacgta gtcttctctt ttttgataac tgtctttccc atttgtcatt
     2101 tgttttatca tcgaaaggtt ttaataaatt ttaaagaagc aaatgtatgt aatttaatta
     2161 taatacaaaa tttttaagga aaaaataaat atgtttgcat aaaataaatt ataagtcatt
     2221 gaaagacaaa tgcaaataaa atttaggatc gaaaaaagtt tacaattttg tgcattaata
     2281 tttcaaaatt agatacttaa cattccaaaa atagtcaagt atcataatag atatttaact
     2341 aatgtgttca aaatcagaca acttcttttc ccaaaaaaaa tttccggttt ttaaaaattt
     2401 actgtttact atttttgctc atgcacaatt tttcactcag taagaattct tatatcgatt
     2461 ttaggaataa aatcaatgcc tggtatcatc ccgattttag aaaaatgccc tggaagggag
     2521 aaaaggatcc ttacaagata tggatttcag aggttatcct ccagcaaaca cgtgtacaac
     2581 aaggttggga gtattatgtg aattttctgg agaagtttcc caccttacag gtattggctt
     2641 cttctgatat agacgatgtg ctgaaaacat ggcagggctt gggttattac agtcgtgccc
     2701 gtaatctcca tctgggggcg cgccgggttg tagagcattt tggtggaagg ctaccggcca
     2761 attaccatga tttattgaag atccccggca tcggtcctta tacagccgcc gccattgctt
     2821 catttgcttt cggactcccc tatgcggttg tggacggcaa tgtggttagg gttcttgcgc
     2881 gatatttcaa tatcgaggct gcatttgatt caacagcagg aaggaagatt ttttcaatca
     2941 ttgcaggaaa tgtcctggaa cctgctgatc ccggtcgatt caatcaggca atgatggacc
     3001 tcggagccac aatatgtacc ccgacagcaa cacgatgtgg catttgtccc ctcagagatg
     3061 gttgcatggc atttaaattg gacaggataa aacagctacc aatcaaaccg aaaaagataa
     3121 tccgccggga aagatttttc aattacctca taattcgttc cacagactca tgcttgttgt
     3181 ttcgtcgcca aaaaggagac atatattcag gtatgtatga atttcttttg tgggaaaatg
     3241 agaaggaggc ctcgacaatg gatatggggc aatggcttca ggacatagga ataaatggcc
     3301 gggcggaacc tatactcaag cttcctcctc aaatcctgac tcatcagaag ataattgcca
     3361 ccttctatat tatacatgta gctgatttgc ccacgttgga gtcagccatt gaggttgctt
     3421 tcaatgacct tcctcaattt gcctttccaa aatttatcca gactgccatt gatcggatag
     3481 atttgaagaa aattgaggga gtgattcagc ctcaaatatc tcacaatagt tgatattttg
     3541 ataactgatc attagatagc aaggatagac cattccgaca caatgagacc ttcaatatcc
     3601 tgttagccca tccagttgca tattaaccaa agtttcaatt ttacaaaaca aggatttatc
     3661 tatagtttga ctgaaattgc tatagatgga atgtgttgtt cgatcctctg tgtccatcgg
     3721 gaaatattct atttagagca ttacatgttg gcatattgat tcgccggatg agcaaagaca
     3781 aagatgaagg cgatgtgaaa taactcctcc tatgtatttt ataatagata ctttagggat
     3841 aaacggggtc ttctatactt atttagagac cggttcttgt gatgttaact aagcgtttct
     3901 gtcaaaaaat tcaaatattt gactgaatgt ttggttacaa tgcaatatat ttatcattta
     3961 ttaaaaaaat taagtattat gaaaaagatt ctcttgttgt gtacattttc ttttctaacc
     4021 atctccatat cttttagcca aaaaggttca tccttcatca ttgccaatat cggtgtagat
     4081 aattacaaga ttgtaaacaa tgaatacact aaaaccttca aagttggtgg tggatatggc
     4141 tatagtatta ctgagccgat ggcattgggt gcagagttca gctattcaac ctttaaaata
     4201 aaaaacgcac tcactacgtc ctcatcaacc agttggtcag caggaccctt tatccgttca
     4261 acttttccac tcagtgacat gatttctttt tattctcaat tgggtgcagg gtatcagacg
     4321 gaagggaata agaccgcatc aggattttat gcctatctga atccggctgc catgctgaag
     4381 gtggacaaag tgacatacat taccttcaca accggcctca tcagatacga taatattaaa
     4441 tcattttata gtcgtttccg ttttctatat gggaatccaa ctctcggcgt gatgtttaaa
     4501 atcaacggga aataaatatt cgaaggcaaa ttttggttca cagaattgta ttttgggtaa
     4561 gtaaaggggg cattaaaagc acggcttacc gtattgcctt ttcaagcctc attccggcta
     4621 ctatcaggtc ataggctgcc tgaaggtatt cggcttttgc ttgtctataa tgcaactcac
     4681 tggtcgaaag ttcggaaacc attacacgac cctccttaaa ttgatctaag gataacttga
     4741 aattcttgct tgccagttcc attgattgtt ccatgagatc aagatctctt ttccgaaaca
     4801 aatagtcatt gcgggcttgt gatacttcaa agttgtgttg gttctgctcc gtgagatatc
     4861 tattccggtc ggccaatatc tgcaacttca gtacatcagc cttttttata cgatccaatc
     4921 cttgtgtgat aggtatcgtg acggaaaggc ccagatagct gttgccatac caatgacttt
     4981 cattgaagat attaaagcca ttgtcataat aattggcgcc caaaaaacca ttgagtgaaa
     5041 ccgttggcag aaagccatcc cttgtatttt gaagtcggat ctcagagaga gtctgctgat
     5101 gagcgatcct ctgcagatgg agtggctcgg gagcattgtg gacgtctttg acaaataatg
     5161 tcattaatac cggaatacta tcagtaagta ttggcatttc agattgcgca gggtctaatc
     5221 cgagatctgt aagcaacctc gctcctgcct gtgccaatat attttcggcc ctgaaatagt
     5281 cagacactga cctattctta tttgccatgg cgttgtttat atcggcatcc gtagatctac
     5341 ccgctttgta tctgtcactt gtcacggaga gtatgcgggt cataatagcc gtgtcgaggg
     5401 cagccattac cacttgctta cgggcgatga tacatgccac atagtcgaga tttatcgcaa
     5461 actgtagatc tgatgccgat atggcggcct ctaccgtact gataagagtt tgatgctcgg
     5521 cttcacgtac agcaccgcgt gtctgtggat tgaacaggtc ataatgggca ttgaggccaa
     5581 tgttgctgct ccatcggctg gcaaacttca agggtaaaag cacattctcc gaagctgccg
     5641 gatcaaaggc gcgcgccgga acaggagttg tcggaataat taggttgctg cggatatcgt
     5701 aattggtata tacttgcggg attttcttcc atcgggcttc ctcgacttgt ttttggctta
     5761 tggcgatatt aagattgctg ttgaggatgg ttggattgtt tttaatagcg agttgtcgtg
     5821 cttcttccaa cgaaagggtt gccggtatgg atggtagttg tccctggcta tattgtattg
     5881 aggtaatcag gccaatcagg acgaataact tcactttcat ttttagtaca atttgttatt
     5941 tgaaaactga agcaggctcc agtttattga tttttgaaat ggcaaacagt gagccgccca
     6001 cggaaataaa caaggtagca aacaagagaa atcctgccag tgaaggcgta atctgtacaa
     6061 ttaatccggc tttggccatg ccttgcttga aaaagaatag caatgtcaag gcgataacat
     6121 agccggtaag cgcaaataaa atcgcctgtg caaggatcaa tcgccgtaca taaccattcg
     6181 tcgcaccaat ggccttatag gttccgtaat cccgcaatcg atccaacacg gcggagtaca
     6241 atgtcaggcc tatgataaag aaacctgtga tgatggcaaa aaccacaagt gttccgaagc
     6301 taacacccat attcgtggaa ctgagcaatt cgcgtactgt cgattggcgc aaatcctgca
     6361 attcccaagc cttgacacca taaaaagttt ggttgatatc tgaagccacc tttctgacat
     6421 ttacaccggg tttgattttt acggagatta tacttacttt gtcattggga taattgccat
     6481 aaaagcgggc gttgtctata ttcgaatata agaagtggcc accaaaagct tgtatgtgcc
     6541 tggtcttcaa ccgaatgatg gcgctcttgc cgttgatttc aaggcgggga cctatattta
     6601 aagggatttt tagtgaacgg gagtcaaaat attcggcaga aaccgagttg gcgcgaatca
     6661 gcgctgacag atctccttct gtaatcctcg ccgtgtcggg tcctccggca aatgaaggtg
     6721 cttcactgcc gatgattgtg aagggtgtcg tgcggccatt agggagagtg acactgactg
     6781 aagtaacgac gatggggtaa gtatgctgca cacccggaat acttcggatt tcatttacca
     6841 ggcgggaatc cagacggaac aggttattga cattggctgt ggtattgtcc acgacccaga
     6901 tctcaccggt attgtgatta gcatggtcga ttaatgcacc catcacattg gtgatgaaat
     6961 ggagtgtgcc caactgctgg ccaatcagga aactgcttat gacgatacca acgatgatgc
     7021 ctatggactt tggccggtca aaccagataa atttaatggc agtgcgaaac atgattaatg
     7081 gtctttcaga tgaatctcac attctacctt ggtattgagt agcataggac ggtctgaagg
     7141 atcgattctg atcttgaagc ggcgtacgcg cctgtcctgt gcctcgccgg gcatatcggt
     7201 aaaaagagat ttgttgttga gctgaggcga gaggaatatg atttttccgc ttccaacgat
     7261 ctgtttatcc cccatcaggt ggagggttgc ctcctgtcca agagctagtc tgtccgcaaa
     7321 caattcatcc acctcaccat gtaccactaa tgggcccctg ggagctaagt aggcataggc
     7381 ctgaagaggt tgaattgccg aaccgggttg tgcgttctgg ctaaggagaa taccggctga
     7441 aggggatcgt atggttagcg agcccagatc atgttcagca agacggagat cagcttgtag
     7501 ctcatccatc ctggagcggc ttatgacgac tgcctgtcga ccttgttcga tctggttttt
     7561 gagtacatca atttcggaag cttgattagc tatgttttga agtgtttcgg cacctgattg
     7621 ggccagcttc cttcctgcct ccagattggc agtcttataa ttaagttggg cttcatattg
     7681 acctattgct gtctgatttg cgacgatttg ttgtcgttcc gtcgcaatct gggcctttat
     7741 tttacggatt ttaagggaag cattttcggg ctgcagtgta aatatggcct ctccggcttt
     7801 taccgtatcg ccttcctgcc ggaaaaccga attgactata cctccagtgg atgacgcgag
     7861 ttgaatcact ccatctaagg gttctacttt gccgatgccc acaattattt caatgttgtg
     7921 tggtaccgaa agggtatcgg acgcaatggt tgcctgccgg cttggttgtt tgttgctaca
     7981 tgacgttatc aagaagagaa tggtcaaagc aaggaaattt ttcattgaat ctatcatgat
     8041 aacacgatta ttgttcttct ggaaatgggg tttcggaagt tctgccttcg gacagataga
     8101 taattctgtc tgcatatttg attagacggg tgtcgtgggt gacgacaatg accgacttgc
     8161 cttgcatgga caatgttttc aactcttcca ttacttttga cgcgtttttg gaatcaatgg
     8221 aagcggtcgg ttcgtcgcag agcaggatgg caggatttgt cactaaagcc cgagctatgg
     8281 cagttctttg tcgctgcccg ccactgaggg tgtggggcag gttattcatt tttgaaatca
     8341 tatcgacctt ttcaagggct atcatcgctc ttgctttcgc ctgtcgccgg ttttcgccct
     8401 ggagaatgag cggcatcatt acattttcca atgctgtaag tggctggatc agattgaagc
     8461 cctggaaaac aaagccgatg ttattgagcc gaatgcggga caactttttt tcctttaatg
     8521 taccaatatt ttgattcatg acaatgacat ctccggtcgt tggataaatg acacacccca
     8581 gcaatgaaag cagtgttgtt ttcccggaac cggacggacc aaggataagg gtcaattcgt
     8641 gcgaatgaaa ttcgagcgtg gtctctgtta gggctgtgat gtccgtatca cccattttgt
     8701 atttttttga agctcttctg atggatgcca caatctccat attaaatacg atttagtatt
     8761 atatattgca aatataaaat gttttcgtat tatttaataa aactacaatg gaaaatcaaa
     8821 atcggatatt gaaggatgaa ttctccggat ggacagaaag gttgatatgg ggaaattgta
     8881 ttgcagaaag ttattcaatc ctcattcagc aaaaaattcc tgatcttaaa tcaaaaagtg
     8941 gtatttgtga catgaataca gcttttgaag accaagggaa tacgatttta tttttttgga
     9001 tgtagtcatg gcgcctttat tttcaagtag gtattatttt aataaggagg gaagtgtagt
     9061 tatagaagag attatcagac aaaataatta attgatttgc tgattttctg tgtggatgta
     9121 acaaattgga cgaaaaggct tgttgttctt aaagtgttgt ttattaatta tctacccact
     9181 tagaatttta ttaaattttc tgtccagtgt aatcggactg atgatttctt ttagtgttaa
     9241 ttatttattc ctatgtttgc gttaacaaaa cctttttctt ggactgaatt tttcactttc
     9301 caaaaatcca ataatgcctg atagtctgaa accttgccca caatgcaact atccatatgc
     9361 ctatgcttcc gaaggcggac ttttttcatg tccggaatgt ggtcacgagt ggaatccgga
     9421 agaaatttac tctttggaag atgttctaag tgtttttgat gcaaatggga atgaacttaa
     9481 aaacggagat accgttgtga ttatcaagga tttacctgta aaaggagctc ctaagccaat
     9541 aaaggcgggg acaaaagtta aaaacatccg gctttcagaa ggggaccaca atataagttg
     9601 taagattgat ggatttgggg ctatggggct caaatccgaa tttgtaagaa aagcctgaat
     9661 ttatataaaa gcctatttgt tttcattcac tttttgaggc tgacagcttc agtaagtatt
     9721 aatgcaggat gttcaatcta ggttttttgc atgtagaatg aacagttttc aggaaaccaa
     9781 tttcttaccg gtcatcggcc tatgcctgac cattctggcg atgatgtcat ccaggatttc
     9841 acctttcatg cggtgcctgt tgtagcggta gcag
//
LOCUS       DAOQQN010000067         9839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2549_length_9839_cov_5.840249, whole genome shotgun sequence.
ACCESSION   DAOQQN010000067 DAOQQN010000000
VERSION     DAOQQN010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9839
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2549_length_9839_cov_5.840249"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="PLG24_03925"
     CDS             complement(<1..1176)
                     /locus_tag="PLG24_03925"
                     /inference="COORDINATES: protein motif:HMM:NF015592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HMZ24065.1"
                     /translation="MKKYIEIHPWKIIETDFSLNDNMVTESLLAIGNGRMGQRAVFEE
                     TFTGETLRGSYLAGIYYPDKTRVGWWKNGYPEYFAKVLNAVDWLGLNIQLDSTIIDLG
                     KLQPLDYRSELDMEKGLLSRTFGLQLPNDVYVRIHSERFYSKTHPDSALLHYRIKVEK
                     GEVDFTLDSGLEADVRNQDTNYGEAFWENFKSEITGNSGIVTAQTKKTGFRIAASTAH
                     KVMVNGWAINGGLDTPSRSSINANFSTFLSQDDEVIIEKYVSITTSFFYPHDDLTIKS
                     LENLDEITQLGYDELRDKQVEAWRKSWDQNDIVIEGDDEAQQGIRFNIFQLNQTYTGE
                     HALLNIGPKGFTGEKYGGATYWDTEAFAVPLFLSVADPEVTKTLLQYRHNQLPGAYHN
                     "
     gene            complement(1223..1900)
                     /gene="pgmB"
                     /locus_tag="PLG24_03930"
     CDS             complement(1223..1900)
                     /gene="pgmB"
                     /locus_tag="PLG24_03930"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HMZ24066.1"
                     /translation="MKLKFNEVGFIFDLDGVIVDTAVLHYKAWRRLANSLGFDFSEVR
                     NEELKGVGRMDSLDKILSWGGITMTQEQKLQAAERKNEWYGELITHLGPKDALPGAIN
                     FLEKCADKGIAIALGSASKNAMPVLNSLEITDYFDIIVDGHSTTKNKPDPEVFLKGAS
                     GLNLVPSLCAVFEDAAVGIEAACLAGMTAIGIGKKENLPKANQVYCCLAEANMDTILD
                     LIALNHM"
     gene            complement(1979..2833)
                     /gene="nadC"
                     /locus_tag="PLG24_03935"
     CDS             complement(1979..2833)
                     /gene="nadC"
                     /locus_tag="PLG24_03935"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HMZ24067.1"
                     /translation="MTDIALINDFITRGLQEDTRGFDHTSLACIPADDTSDAWILIKD
                     NGVLAGVELAKMILLHVDPHAKVDILIQDGTAVEYGDIALTVTANSRSLLMAERLLLN
                     VMQRMSGIATLSRRFASEVSDLPVKILDTRKTTPLLRYFEKWAVRIGGCNNYRDGLYD
                     WIMIKDNHIDAAGSITGAIRSVNEYLKTNNLDLGITVEVRDFEEIREVLAVGNVTRIM
                     FDNFGLADLSKAVELVGGRFETEASGGVRLNTVRSIALTGVNFISVGALTHSATSLDI
                     SLKFRKGS"
     gene            complement(2843..5812)
                     /locus_tag="PLG24_03940"
     CDS             complement(2843..5812)
                     /locus_tag="PLG24_03940"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMZ24068.1"
                     /translation="MKKIILHLLLVAFSSFNLYSQSEYWQQKVDTRINVLLNDSTHFL
                     HGNIAISYQNNSPDRLDFIYMHLYPNAYRNVNTAFAKQQLEARSTNFFFSEKWQRGYI
                     DSLDFKVVSNGNTESAKIENTQNIDIIKLMLPHALAPGETITIKTPFRVKIPYVFSRM
                     GHNDQSYQISQWFPKPAVYDASGWHPMPYLDQGEFYSEYGDYEVSITLPDNYIVMGTG
                     NIQEATENEWLDRLSQVKVDSMYISQHKDKPKSSPNQKTITFRESNIHDFAWFADKNW
                     VVRKDTVMQPDGSGIVTAYSAFYPKHYDAWKNSVNSIKKTIRGYGSKVGIYPYETVKV
                     VEGSLKAGGGMEYPTVTVIEASNNANTVDKVIVHEVGHNWFYGILGSNERDYPWMDEG
                     INSFYEGKFTGAGNRLQIFDKMDENNMAYTALGASRKLYPATMKSTMYPTINYGVDIY
                     MKVPLYLNYLEAYLGVEEFDRNMHEYFRTWQFKHPQPLDFESLFKKNSTKDIQWFFDM
                     MNSGEMVDLAIVNTQKTGNLTQVTIRNNNSFALPVHVYAESGGLIAAQQWTEPFVGEK
                     VLAFDNIAEPARFYIAPSIPNGNLKRNSSERKTRLKPLVNVNFNEYKTIWYSPAIGYN
                     YYDGFMAGLLLHNVTLPENKVRFYIAPMYGFKSGNVVGSGVINYTTHLKNKTIDNLDF
                     YLYFKKFGYNRTFYEDRSNPSLDYFKIAPEMVINFTKPNLRSPASSSLSLKGYYIGEQ
                     QFIFDTIPGTEPKKGAYGSKYYGKITYRFVNNKVINPSNYTVEAQAGAAFVKLSAEGN
                     WKFNYTVPKKAFHLRAYAGKFIKLTDSYFDYYRYRLSNTYTGSNDYLYDYTFGGRHEQ
                     HGFWSHQVGIREGGFKFATPLANNAPGTNDDYLIALNMKTDIPIKGVPIRLYCDVSKY
                     GGPVDKEYSGFLYEAGVEVHLLDYITVHIPIFMSKEYKEYKKFNLGNNFFKAISFSHN
                     LNSINWVRPTDIIYNLF"
     gene            complement(5881..7146)
                     /locus_tag="PLG24_03945"
     CDS             complement(5881..7146)
                     /locus_tag="PLG24_03945"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HMZ24069.1"
                     /translation="MNTNSINILSTKRYITICLLAFGLLCSGASSIAQQSHISKMKYT
                     ISGLANETIQRGEPFDGMVIIFDSEQTQPSFIWDQSGIPYDLTIDEHIEDRIQSNLII
                     FPHRQNRVTLTGIDEGQRLLIYFITIPEYSKSLTAYRSNDDCSEPAVVPQSIWRAGLN
                     PPVPGRKATPTRHCIVHHSASSNADTNSLKLIRGFYILHTEINGWDDIGYNYLIGYDG
                     TIFAGRDPEKPEIRQDNVFGAHFCGKNSYTMGICVIGDFEAEAPDPRAVVALRHLLSW
                     KMFKDKMNPLDSLHHPDTINGGLLPVLAGHRNGCATACPGQLLFDMLPQLRQDVKADL
                     EACTQLNTFDKSQPTLQVFPNPAHEAVSIKFPGNFQYELFNVNGFTARNGTGNDELRL
                     DVSGFTSGIYLLKVKSGVNTQNATIVILR"
     gene            7663..8931
                     /locus_tag="PLG24_03950"
     CDS             7663..8931
                     /locus_tag="PLG24_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HMZ24070.1"
                     /translation="MSSSKIYCSSEIGKLKKVIVHRPDNGIARITPKEAAELLFDDIV
                     YLPQMQKEHDTFTSVLKAFVGEENVLFVADLLEQALAADDIKKTELIRMIVDYEEIPQ
                     SFGNIFTELSNSKLAEVLISGYLEEEDHYFFDPIPNFIFTRDIAITVKDHVIIAKAAK
                     EARFRENLLARFIFWAHPMFQELQDEGRVINLNWLESFPPSKKGEWVNLEGGDTMIFE
                     NDYLLIGHSERTTQHAIHSLAKVVLEKGLVKNVVQVNIPFERYCMHIDTVFTRISKDH
                     VVAYKPLIFDGISSNVEVFTHDGQHKKYNSLKDFILAEINPDMKFIFSGDGESPYQER
                     EQWTDGCNLVAIKPGVAITYDRNPITEEAFRKAGFNILHANDFLVQYKSGKLDPWQLE
                     NTIITLPSNELSRGRGGSHCLTCPIEREIM"
     gene            8947..9357
                     /locus_tag="PLG24_03955"
     CDS             8947..9357
                     /locus_tag="PLG24_03955"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HMZ24071.1"
                     /translation="MYEYSHSLRVRYGETDQMSYVYYGNYALYYEVGRVETMRSLGLS
                     YAVMENEVGVMMPVMRMDIEFLRPAFYDELLTVKTSIPELPDRDIIFQSEIFNGKGKI
                     VNRGHVTLCFVSKETKKRISAPELLLDKLRQFYV"
     gene            9350..>9839
                     /locus_tag="PLG24_03960"
     CDS             9350..>9839
                     /locus_tag="PLG24_03960"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HMZ24072.1"
                     /translation="MFNRKSDSRLAAFMREQTVYFELIDWSRIHTLPGLRGQSIYRIF
                     SELGVHLKRSNIALKASAISFSFMVASFPFMIFLLTLLPYFHIQNAVDVLKSNIGNLL
                     PTNVENTLFGLIEDTIKPRGSLLSIGFISALFFASNGVLNLMSAFDQSERAKTLEINW
                     VKK"
BASE COUNT         2633 a         2061 c         2172 g         2973 t
ORIGIN      
        1 attgtgataa gctccaggga gctgattgtg acggtattgc agcaaggttt tggttacttc
       61 tggatcagca acagataaga aaagcgggac tgcaaaagcc tctgtatccc agtaggttgc
      121 tccgccgtat ttttcaccgg taaagccttt gggaccaata ttcagaagag cgtgctctcc
      181 ggtatatgtc tgattaagct ggaaaatatt gaaccgaatg ccctgctgag cctcgtcatc
      241 tccttctatc acaatatcat tctgatccca acttttgcgc catgcctcta cttgcttatc
      301 gcgcaattca tcatagccaa gttgtgtgat ttcatccaga ttttcaagag atttgatggt
      361 aagatcatca tgcggataga aaaacgaagt ggtaatggat acatattttt cgataatcac
      421 ctcatcatcc tgagatagaa aggtggagaa attagcattg atgctactgc gggagggcgt
      481 gtcgagacct ccattgatag cccagccatt gaccatcacc ttatgggcag ttgatgctgc
      541 tatcctgaat cccgttttct tggtttgtgc agtaacaatg ccggaattgc ctgtgatttc
      601 agacttaaaa ttttcccaaa acgcttctcc gtagttggtg tcctgattcc tgacgtctgc
      661 ttccaatcct gagtcaagcg tgaaatctac ctctcctttt tctaccttaa ttctgtaatg
      721 caacaaggca gaatccggat gagtcttgga ataaaatcgt tcagagtgga tacgtacata
      781 tacatcattg ggcaattgta ggccaaacgt tcttgacaaa aggccttttt ccatatccag
      841 ctcacttctg taatccagag gctgcaactt gcccaggtca ataattgtac tatcaagctg
      901 aatgttgagt cccagccagt ccacagcgtt caataccttg gcgaaatatt cagggtagcc
      961 attcttccac cagcctaccc gggttttgtc gggataatat atgcctgcca gataagagcc
     1021 acgcaatgtc tcaccggtaa atgtttcctc gaaaacagct cgttggccca ttctgccatt
     1081 acctatggcc agcagggatt cagtgaccat attgtcgttg aggctgaagt cggtttctat
     1141 aatcttccag gggtgaattt ctatatattt tttcattgct aattcattca taaataagat
     1201 tggaattttg gtgaaacgga cactacatat ggttgagagc gattaaatcc aggatggtgt
     1261 ccatatttgc ttctgcaaga caacagtaca cctgattggc ttttggtaaa ttttcttttt
     1321 taccgatacc gatagctgtc attcctgcaa ggcaggctgc ctcaatacct acagctgcat
     1381 cttcgaatac cgcgcataat gagggcacaa gattgaggcc ggatgctcct tttaggaaaa
     1441 cttcagggtc aggtttgttt tttgtggtgg aatgtccatc gacgatgata tcgaaataat
     1501 ccgtaatttc caggctgttc aggacaggca tggcattttt tgaagcagag ccaagagcaa
     1561 ttgcgattcc tttgtctgca catttctcta agaaattgat tgctccgggc aaggcgtctt
     1621 tcggacccag gtgagtgatc aattcaccat accattcatt tttccgttcg gcggcttgta
     1681 atttttgttc ttgtgtcatg gtgattccgc cccaggagag gattttgtcg agagaatcca
     1741 tacggcctac gccttttaat tcctcattcc ggacttcaga aaagtcaaat cccaggctat
     1801 tggctagcct gcgccatgcc ttataatgga gcactgccgt gtcaactatt actccatcca
     1861 ggtcgaaaat aaatcctact tcgttaaatt ttagcttcat tcaggccgtt attatcaagt
     1921 ggccttgcta aggtatgaat tatatataca tattgtgtaa aaagtacaca ataaaaaatc
     1981 aggatccttt tcggaacttc aaactgatat ccaaactggt ggctgaatga gtgagggcac
     2041 cgactgatat gaaattgaca ccggtcaatg caatgcttct taccgtatta agcctgaccc
     2101 ctccggaagc ttcggtctcg aaccgaccgc ctaccagttc gaccgccttg gataagtcgg
     2161 ccagtccaaa gttgtcaaac atgatgcgtg tcacgtttcc gacagccagc acctctctga
     2221 tttcttcaaa gtcacggacc tccacggtaa tgccaagatc gagattattg gtcttcaaat
     2281 attcatttac actgcgaatc gcgcctgtta tacttccggc tgcgtcgata tgattatcct
     2341 tgatcatgat ccagtcgtac aaaccatcgc ggtagttgtt acatccaccg atgcggaccg
     2401 cccatttttc aaaatacctc aacaatggtg tcgtttttct ggtatcgagg atctttactg
     2461 gaaggtcact aacctcagag gcaaacctac gactgagcgt ggcgatgccg ctcatgcgtt
     2521 gcatcacatt gagcaagagc cgctccgcca tgagtaatga tctggagttg gcagttaccg
     2581 taagtgctat atcaccatat tcgactgcgg taccgtcttg tatcagaata tcaaccttgg
     2641 catgtgggtc cacatgaagc agaatcattt tggcaagttc gacgccggcc agcacgccat
     2701 tgtctttgat cagaatccag gcatcacttg tgtcatcagc cggaatacat gccaatgaag
     2761 tatggtcaaa ccccctggta tcttcctgta agccacgggt tatgaaatcg ttgatcaatg
     2821 ctatatctgt catttttctt gattagaata aattataaat gatatctgtc ggtctcaccc
     2881 aattgataga attcagatta tgggagaagc taatggcttt gaagaaatta ttaccaagat
     2941 tgaatttttt atactcctta tattccttgc tcatgaatat aggaatatgc acggttatat
     3001 aatcgagcag atgcacctct acacccgctt cgtataggaa gccggagtat tccttatcca
     3061 ccgggccgcc gtatttggaa acatcgcaat ataaccttat cgggacacct ttgataggta
     3121 tatcagtctt catgttgaga gcgatcaggt aatcgtcgtt tgtacccgga gcattattgg
     3181 ccaatggagt ggcaaactta aagccgcctt ctctgatacc tacctgatga gaccagaatc
     3241 catgctgttc atgtcgaccc ccaaaggtat aatcatacag atagtcattg cttccggtgt
     3301 atgtattaga caatcggtac cgataataat caaaatacga atctgtcagc ttaataaatt
     3361 ttccggcata ggctctcaga tggaatgctt ttttgggtac tgtataatta aacttccagt
     3421 ttccttcagc ggaaagtttg acaaatgctg ctcctgcttg cgcttctacg gtataattgg
     3481 atgggttgat taccttgtta ttgacaaatc tataggtaat ttttccataa tatttgctgc
     3541 cataagcacc ttttttaggc tcagttccgg gaattgtatc gaaaataaat tgctgctctc
     3601 cgatgtaata tcctttgagg gacaaggatg aactagccgg gcttctcaaa ttcggtttgg
     3661 taaaattgat aaccatttca ggggcgattt taaaataatc cagtgatgga ttgcttctat
     3721 cttcataaaa agtcctgtta taaccgaatt ttttaaaata caggtaaaaa tcgaggttgt
     3781 ctatggtctt gtttttcaaa tgtgtggtat aattaattac gcctgacccc acgacattac
     3841 cacttttaaa tccatacata ggtgcgatat agaaccgaac tttattctca ggaagggtga
     3901 cattgtgcag caacagcccg gccatgaatc catcataata attatatcct atcgcgggtg
     3961 aataccagat ggttttgtat tcgttgaaat tgacgttgac caatggtttt aatcgtgtct
     4021 ttcgctcgga agaatttcgt ttcagattgc cattcggtat ggagggggct atatagaatc
     4081 tggccggttc ggcgatattg tcaaatgcga ggactttttc acctacaaac ggctctgtcc
     4141 attgctgtgc ggcaatcagc ccaccactct ctgcgtatac atgtaccgga agagcaaagg
     4201 aattgttgtt tctgatagta acctgagtta aatttcctgt tttttgagta ttgacaatag
     4261 ccagatccac catttcacct gagttcatca tgtcgaaaaa ccattggata tctttggtgg
     4321 aatttttctt gaacaaggat tcgaaatcta acggttgggg atgcttgaat tgccatgtgc
     4381 ggaaatattc atgcatattc ctgtcaaatt cttccactcc gagatatgcc tcaagatagt
     4441 tgaggtataa aggcactttc atatatatgt ccactccata attgatggtc gggtacattg
     4501 tgctcttcat tgtggccgga tatagcttgc gggaagcgcc aagggcagta tatgccatgt
     4561 tgttttcatc cattttatca aagatctgca atcgattgcc tgcgccggtg aattttccct
     4621 catagaagct gttgatgcct tcgtccatcc agggataatc cctttcattg gatccgagga
     4681 ttccatagaa ccagttatgg ccgacttcat gcacgatcac tttatccacc gtattcgcat
     4741 tgttggaagc ctctatgacg gttacggtcg gatattccat tcctccacct gccttgagtg
     4801 agccttccac gactttcact gtttcgtagg gatatattcc caccttgctt ccatagcccc
     4861 ggatggtttt tttgatggaa ttaacagagt ttttccaggc gtcgtagtgc tttggataga
     4921 aggcagaata cgcggtcacg atacctgaac catccggttg cattaccgta tccttcctta
     4981 cgacccagtt tttgtctgca aaccacgcaa agtcatgtat gttgctttcc ctgaatgtaa
     5041 tcgttttttg gttcgggctg cttttgggct tatccttatg ctgactgata tacatactgt
     5101 caactttgac ttgagatagt cggtctagcc attcgttttc tgtagcttcc tgaatatttc
     5161 cggtccccat gacaatataa ttatccggaa gtgtgatgct gacttcataa tccccgtatt
     5221 cactgtagaa ttctccctga tcgaggtagg gcatcggatg ccagccggaa gcgtcgtata
     5281 ccgctggttt gggaaaccac tgggatatct gatagctctg atcattgtgc cccatccgtg
     5341 agaaaacata tgggatcttg acacggaatg gtgtttttat tgtgatagtc tcgcccggag
     5401 ctaaggcatg tggaagcatg agcttgatga tgtcgatgtt ttgcgtgttt tcgattttgg
     5461 ctgattcggt attgccattg gatacgactt taaaatcaag gctgtcgatg taaccacgtt
     5521 gccatttttc agagaagaaa aagttggtag atcgggcttc aagttgttgt ttggcaaatg
     5581 cagtgtttac atttctgtag gcatttgggt agaggtgcat atagatgaaa tcgaggcggt
     5641 ctgggctgtt gttttggtaa ctaatggcga tgttgccatg cagaaaatgg gtggaatcat
     5701 tcagcaatac attaatccgg gtgtccacct tctgttgcca atactcggat tgtgaatata
     5761 gattgaacga actaaaagca acgagtagta agtggagaat tattttcttc attggtaaat
     5821 ttttctcttt aagcataata cgtaaatatg aatgaatttg tcggtcgtaa gaaattatca
     5881 ttatcgtaag atgactatgg ttgcattctg tgtgttcact ccggatttga ctttcagtag
     5941 gtagatgccg gacgtaaagc cagatacatc aagacgtagt tcgtcattgc ctgttccatt
     6001 tcgtgcagta aagccattca cattaaataa ttcgtattgg aaattcccgg gaaacttgat
     6061 ggacaccgcc tcatgcgccg gattgggaaa tacttgtaat gttggctggc tcttgtcaaa
     6121 agtatttaat tgtgtacacg cttccaggtc agctttaaca tcttgtcgta actgggggag
     6181 catatcgaaa agtaattgcc ccggacaagc tgttgcgcag ccatttctgt gtccggccag
     6241 taccggcaat aatcctccat tgatggtgtc aggatggtgg agtgaatcaa gagggttcat
     6301 cttgtccttg aacattttcc aggacaaaag atgcctcaag gcaaccacgg cacgaggatc
     6361 cggtgcctcc gcctcgaagt cgccaatgac acaaatgccc attgtatagg aatttttccc
     6421 acaaaagtgc gctccgaata cattgtcctg acggatttcc ggtttctccg gatccctgcc
     6481 ggcaaagatg gtaccatcgt atccaatcag atagttatag cctatatcat cccacccgtt
     6541 gatttcagta tgaaggatat agaatcccct tatgagcttc aatgagttgg tatccgcatt
     6601 ggaggaggcc gagtgatgga cgatgcaatg ccttgtaggg gtggcttttc gtccgggaac
     6661 cggtgggttg aggcctgcgc gccaaatgct ttggggcaca actgccggtt cggaacaatc
     6721 atcattactt ctgtaggctg tcagggattt gctatattca gggattgtga tgaaataaat
     6781 gagaagcctt tgaccttcat cgattcctgt caatgttacc ctgttttgac gatgcgggaa
     6841 gataatcaga ttactttgaa tcctgtcctc aatatgttcg tcgatggtca aatcatatgg
     6901 aatgccactt tgatcccaaa taaatgaagg ctgtgtctgc tctgagtcaa agataattac
     6961 cataccatcg aatggttctc ctctttggat agtctcattg gcgagacctg aaatagtata
     7021 tttcatcttg ctgatgtggc tctgttgagc aatgcttgat gcacctgaac acaacaaccc
     7081 gaatgcaagc aagcagattg tgatatatcg ttttgtggat aagatattaa tgctgttagt
     7141 attcattgta aagcaaaagt aattcaattt ggggatactt cgcaatgtgg gagtttttca
     7201 ggcgaatttt tgggaaatac aggactatcc ttaggatcaa tcaatctgaa cgggagaaat
     7261 tacttccatg aagaaagcaa attgactgga ttgggcgatt atgcggtcat ctgaaaaggg
     7321 actaaatttg aaaatatttt gaggttgatt tgagcttgta tctgaatttc ttataccaga
     7381 atgatttata taatagtcca taaatttttg gtataatacc gaagttacat tcgttaataa
     7441 aatgattatc acaggacaat gaacgattca gaggacgttg aatttggtag agcaagaaaa
     7501 attttcttag aatcaccaat tgcgttgtca tgattgtaca gtcatataat ttgcttatct
     7561 ttgcctcatg attcttacaa gtatccttat gagaatcgct cttacagctg actttcctgt
     7621 tatgattcgg agccaccccc attgttcaga attatctatt ttatgagttc atctaaaatc
     7681 tattgttctt ccgaaatcgg aaaattaaaa aaagtaatag tacaccggcc ggacaacggc
     7741 atagcaagaa tcacacccaa agaagccgca gaactacttt tcgacgatat tgtctatctg
     7801 cctcagatgc aaaaggaaca tgacactttt accagcgtct tgaaagcttt cgtgggtgag
     7861 gagaatgtcc tgtttgtcgc tgacttgttg gagcaggcct tagctgctga tgatatcaag
     7921 aaaacagaac tgatcaggat gattgtcgat tacgaggaaa ttcctcaatc ctttggcaac
     7981 atctttacag aattatccaa ttctaaactt gctgaggttc ttatctcagg atatcttgaa
     8041 gaggaagacc attacttttt cgatccgata cccaatttta tttttacccg cgacattgcc
     8101 atcacggtca aagatcatgt catcatagcc aaggctgcca aggaagcccg tttccgtgag
     8161 aacctgctgg cccgctttat tttttgggca catccgatgt tccaggaatt gcaggacgaa
     8221 ggtcgcgtga ttaacctcaa ttggttggaa agctttccac cttccaagaa aggggagtgg
     8281 gtcaatctgg aaggaggaga caccatgata tttgaaaatg attacctgct gataggtcat
     8341 agtgagcgga caacacaaca tgccatccat tcgctggcga aggtagtttt ggaaaaaggc
     8401 ctggtaaaga atgtggttca ggtcaatata cctttcgagc ggtattgtat gcacattgat
     8461 actgtattta cacgtatcag caaggatcat gtagtagctt acaagccgtt gatctttgac
     8521 ggtatttctt ccaatgttga agtatttacc catgatggtc agcacaagaa atacaattcc
     8581 ctgaaggatt ttatccttgc tgagatcaat ccggatatga aattcatttt ctcgggcgat
     8641 ggagaatctc cctatcagga aagggaacag tggacggacg gctgtaactt agtagctatc
     8701 aagccgggtg tagcgattac atacgacagg aaccctatta ctgaagaagc tttcagaaaa
     8761 gccggtttca atattctgca tgccaatgac tttctggttc aatataaatc cggaaagctg
     8821 gatccatggc aacttgaaaa cacaatcata accttgcctt ccaatgaatt atcaaggggg
     8881 aggggaggct cacattgttt gacatgcccg attgaaaggg agattatgta atgtaaatat
     8941 tctgtcttgt acgagtacag tcattctctc cgggtcagat atggcgaaac cgatcagatg
     9001 tcatatgtat attatggcaa ttacgcctta tattacgagg taggacgtgt ggagaccatg
     9061 cgctcactgg gtcttagtta tgctgtcatg gaaaatgaag taggggtgat gatgccggtg
     9121 atgaggatgg atatcgaatt tcttcgaccc gccttttatg atgagctgct tactgttaag
     9181 acttcaattc ctgaattgcc tgatcgagat ataattttcc aatcggaaat cttcaacggg
     9241 aaaggcaaga ttgtcaaccg ggggcatgtc acgctctgct ttgtaagtaa ggagaccaag
     9301 aaaagaatct ccgctcctga attactttta gacaaactga gacagttcta tgtttaatcg
     9361 gaaaagtgac tctcgattgg ctgcattcat gagggaacaa acggtgtatt ttgagttgat
     9421 tgactggtca cggatccaca ccttacccgg attgagaggg cagagcattt acaggatatt
     9481 cagtgagctg ggggtgcatc ttaagagatc caatatcgca ttaaaagcaa gtgccatatc
     9541 attcagcttt atggtagcat cctttccctt catgatcttt ctactgacct tgttgcctta
     9601 ttttcatatc cagaatgcag tagatgtatt gaagtccaat attgggaatc ttttgcctac
     9661 caatgttgag aatacgctat tcgggcttat tgaagataca atcaaacctc ggggcagtct
     9721 gttgtccatc ggtttcatca gtgccctctt ctttgcatcc aatggtgttc tgaacctgat
     9781 gagtgctttt gatcagtcgg agcgtgcaaa aactttggaa atcaattggg taaaaaaac
//
LOCUS       DAOQQN010000068         9820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2559_length_9820_cov_5.235535, whole genome shotgun sequence.
ACCESSION   DAOQQN010000068 DAOQQN010000000
VERSION     DAOQQN010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2559_length_9820_cov_5.235535"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2550
                     /gene="ileS"
                     /locus_tag="PLG24_03965"
     CDS             <1..2550
                     /gene="ileS"
                     /locus_tag="PLG24_03965"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HMZ24073.1"
                     /translation="PYTALAQSIVMAADLVPTWFKVEGADAEMSYTAGQKLLPYRIVD
                     EYKGKDLTFIRYEQLLPYAQPDEGDAFRVIKGDFVTTTDGTGIVHIAPSFGADDRRAA
                     MANGIGSLTLVDRQGKFTDEAGPFAGRYVKDYKNEGENFKDVDVDIAILLKQENKAFN
                     VQKFVHNYPHCWRTDKPVLYYPLDSWFVRASAAKERMAELNRTINWKPEHTGSGRFGV
                     WLENVLDWNLSRSRYWGIPLPIWRTEDGSEEIIIGSKEELLQKAEEAFKAGILKIQDK
                     SGAIFTTNTDGHLTYREGFDLHRPYIDEVVLLSKQGKPMYREPDLIDVWFDSGSMPYA
                     QWHFPFENLDKFKANFPSDFIAEGVDQTRGWFYTLHAIATMCFDSVAFKNVVSNGLVL
                     DKDGMKMSKSKGNVVDPFKTLDQYGVDATRWYMITNAQPWDNLKFDLEGIDEARRKFF
                     GTLYNTYSFFALYANIDGFTFSEAYMPLEKRPEIDRWIISKLNSLVKEVRELLSDYEP
                     TNAGRKIEQFVDENLSNWYVRLCRRRFWKGEYTEDKVAAYQTLYECMTTVSKLIAPIA
                     PFFADWLYRNLNGGSGLEKHESVHLAYFPADNEDVIDKNLEKRMEYAQRISSLGLSLR
                     KKEKIRVRQPLQKLIVPALSLDFKTKVEAVRDLIEAELNIKELLVTADTEGLIKKGAK
                     PNFQTLGKRLGKQMKLAQNVISNLSQADIRQFEQTNHYVLETPDGDFDLTLEDVIITS
                     EDIPGWLVAVDNEITVALDIAITPELKAEGIARELVNRIQNIRKNSDFLVTDRINIKV
                     EPIDMTKEAVALFANYIANEVLADSISLEANEGEEVELLDDINIRIQVEKA"
     gene            complement(2625..4904)
                     /locus_tag="PLG24_03970"
     CDS             complement(2625..4904)
                     /locus_tag="PLG24_03970"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HMZ24074.1"
                     /translation="MRYFYTFLAIIAMYGWVAGQAFHGTTGSIADDVTTTTFTCEVSG
                     LPSSMNSVTFGIEQVCVDISHTWDSDLIIKLQSPSGRIVTLIEGAGGDSDDFKNTCLS
                     GSNKNDIFHGWGPFSGTYRPSGDMGLFNDGSNPNGTWKLLILDVYPEDPGKLNSWEIQ
                     FGSNPSKPYLFESSALPIIKIDTYGQSIPDDPKIPASFKIISNDNGALNYVNDTVYSY
                     SGNIGIEIRGSSSQSFPKKSFGLELWDENGEDIKASVLGMPKESDWVLNAHYTDKTLF
                     RNNMTYKLFSEMGHYASRTQLVELFINGEYRGVYTFMEKIKRDKNRVDIPKLSENDTS
                     GMALTGGYIFKIDRINGSGTDGWTSKFEIPGRPFEYTNFLFEYPKDDKINNAQKAYIE
                     SFVDSFETALHSDDFQNPVNGWRKYADELSFMDFMLIQEMSKNVDAYRLSTFMYKTRA
                     DADNKIHFGPVWDFDIAWFNANYCETARPDGWVFNINFICPDGRSPFWWSKILTDTTF
                     ARNMRCRWESLRQTTLSEDHIASIIDGFVAEMQGAENHNFDRWPILGIWVWPNTDPIP
                     TTYQGEIDKLKGWIHQRFEWMDHAIRALSPDISLSINATNDSGLQWHFTPNIDLEGEY
                     KWDFGDGSTSSDVKPDHVFSAPGTYTVTLTYTNGFGCTESVTLTVNVEHSANYGFGDT
                     GVSIFPNPATETISLKTALAFESGNYQISDVHGRKMQSGKILNSGENNIAIGALHPGV
                     YILSLKIDNRAIQQRLVKM"
     gene            complement(4924..7998)
                     /locus_tag="PLG24_03975"
     CDS             complement(4924..7998)
                     /locus_tag="PLG24_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M60 family metallopeptidase"
                     /protein_id="HMZ24075.1"
                     /translation="MIRFVYISLILCVTFGYVYGQYQMPVYYATSYRPGQGGEEVGNA
                     IDGNDATLYHSRYDLYAVPDTLTFYFNPNVPEINKIEYSPRKERYNGIWTKVDIMYSK
                     RDKPDQFIRLNSDPIIWDLNNDVKSVNLDSALEHVFAIRIAVHEAYDDYSSCAEMRFY
                     GTKPALPDGSIDCSLDASTLQGNKDAKIKIDEAGSSASSYQYYENIEKSFDGNFSTLY
                     HSNYNAGQDAFPIDLVYHFSNKPVIDYFIYYPRSDGGYNGFFGATSIYYNTEESGPGA
                     EYTHLLDYDFKMEGLPVRIAFPTSLKVNNVKIVVKNGASGFASCAEMEFYEKNGGDPN
                     VFEYEQIFTDRLYTDLLPLVTQEMIDTISVPFYKQLASCLYAGTYNRSLRIRDFKAFE
                     STGQLSDKLKTDRYDPYENATGILFEAGDKAVIFADGIGGSSVYLRVRNFANESNPAD
                     YLYLLNDGRNVIAMKDSGLAYLAYFSDKPAEVPAITVHIATGKINGYYDPILHNSNDW
                     TTFLANSAYPKIDIVGRYAHLVFDKSALRQNSPFDGNHLIQLYDTIANWQKIQMGLYK
                     FNYNYDNHILCESGTGGGWYAGGIGIHLDLTWGPASITNARQLELWGIPHEFGHINQI
                     RPGLRWIGTTEVTNNVYALWAAYKLNKEGKRFTRLEEEAFRNGNDPKWWAGNRFNIYL
                     DSNYIHGKALQDVANDYHFRVLVPFWQLQLYYQLAGACRNAPELTYEENPDVHGVDYP
                     HWYGIVAEKVRNTNSESLTNGERLLNFVKYTCEAVQEDLSDFFINSGFLRPIDKEIDD
                     YGVGRITVTQNQIDALVAQLRASFPNKPVSPVLHYISAHSMEMYKNKLSLSGRTGEGV
                     DLIIVEDTPYLLIDHNEWKNAVAYETYDSTNTLIHVTITGTADVSNKTTSVLYADQSH
                     KIYAIGYDGKKILVYPKEQSNAVGKNVSLHLKVFPNPTSDGRLVQVETGEAIEIYRME
                     IYDNRGRVVLSGKGNVDKLNHLLRKNLQIAPGAYFIKLTAKHSILSSSFTIQ"
     gene            complement(8358..8849)
                     /locus_tag="PLG24_03980"
     CDS             complement(8358..8849)
                     /locus_tag="PLG24_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24076.1"
                     /translation="MSSLKELEKFNPDSKVWVFTANRVLSDTEITQINSRLEEFAQSW
                     VSHGDPLSARAFVLHRYFVILAVDNKMYLASGCSIDTAMRFIQTLQIDFDVDLLDRQS
                     FVYLKDHKPVLIRRKDMKSAYRDGIILDDTLFFDPLVTSWQEMREGFLKPMRDFWMKN
                     MIR"
     gene            complement(8866..9606)
                     /gene="fabG"
                     /locus_tag="PLG24_03985"
     CDS             complement(8866..9606)
                     /gene="fabG"
                     /locus_tag="PLG24_03985"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HMZ24077.1"
                     /translation="MKLLEGKTAIITGGSRGIGAAIVRLFAEHGAEIAFTYASSAGPS
                     EALVAELSAKTKIKAYQSDAASMSAAEQLVKDVLGDFGKIDVLVNNAGITKDGLLLRM
                     SEEMWDAVIDTNLKSVFNLTKHAIRPMLRTGGSIINMSSVVGVFGNAGQANYSASKAG
                     IIGFTKSVAKELGSKNIRCNAIAPGYIATEMTQVLDESVKEGFMANVPLKRLGEAEEI
                     ANVTMFLASDLSSYITGQVLNVCGGMAI"
     gene            complement(9608..>9820)
                     /locus_tag="PLG24_03990"
     CDS             complement(9608..>9820)
                     /locus_tag="PLG24_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ24078.1"
                     /translation="AVRISWADTAPRDIEIKIFDMQGNLRSVIKPDAGRSFIEINTSG
                     HIPGTYIYQMIGPEKKLLSNGKFILQ"
BASE COUNT         2859 a         2202 c         2034 g         2725 t
ORIGIN      
        1 ccctatacag ctctggcaca aagcatcgtt atggctgctg atcttgttcc gacatggttc
       61 aaggtggaag gcgctgatgc tgagatgtcc tatacagcag gccaaaaact gctgccttac
      121 agaattgttg atgaatataa gggtaaagac cttactttca tccgatacga acaactactt
      181 ccttacgctc aaccggatga aggtgatgcc ttccgcgtga tcaaaggcga ctttgtaaca
      241 actactgacg gtaccggtat cgtacacata gccccttcct tcggtgctga tgaccggcgg
      301 gcagccatgg ccaacgggat aggcagtctc acccttgtgg acagacaagg taaatttacg
      361 gatgaagccg gcccattcgc cggtcgatat gttaaggact ataaaaatga gggcgaaaat
      421 ttcaaggatg tcgatgtgga tattgccatc ctgttgaaac aggaaaataa agcattcaat
      481 gtgcaaaaat ttgtccataa ctatccgcat tgctggagga cggacaagcc ggttctttac
      541 tatcctctgg acagctggtt tgtaagggct tccgcagcca aggaaaggat ggcggagctc
      601 aacagaacaa tcaattggaa accagaacat accggatcag gacgatttgg cgtctggctg
      661 gagaatgtgc tggattggaa cctgagccgt tcgaggtatt ggggtatccc attgcctatc
      721 tggcgtactg aggatggtag tgaagaaatc atcatcggat ccaaagaaga attattgcaa
      781 aaagccgaag aagccttcaa ggcgggaatt ttgaagatcc aggataagtc tggtgccatc
      841 tttacaacca ataccgacgg gcatttgaca tacagggaag gatttgatct gcaccgacct
      901 tatattgacg aagtcgtact tttgagtaaa caaggtaagc caatgtaccg ggaaccagac
      961 ctgatcgacg tttggttcga ttccgggtca atgccctatg cacaatggca ctttcctttt
     1021 gaaaatctcg ataaattcaa ggcaaatttc ccgtcagact tcattgcaga gggtgttgat
     1081 cagacacgcg ggtggttcta tacccttcat gcgatagcca cgatgtgttt tgacagtgta
     1141 gcattcaaga atgtagtctc caacgggctt gttctggata aggacggaat gaagatgtcc
     1201 aaatccaagg gaaatgtcgt ggatccattc aagacattgg atcaatacgg tgtggatgcc
     1261 acacgatggt atatgattac taacgcacag ccatgggaca atcttaagtt cgatctggag
     1321 ggaattgatg aggcaagaag gaaatttttc ggtaccttgt ataataccta ttccttcttt
     1381 gcattgtatg ccaatatcga tggattcaca ttttcagaag catacatgcc tctggaaaaa
     1441 agaccggaaa tcgaccgctg gatcatctcc aaactcaact cacttgtcaa ggaagttaga
     1501 gaattactat cggattacga gccaacaaat gccggccgta aaatcgagca atttgtcgat
     1561 gaaaacctga gcaactggta tgttcggctc tgcagaagga gattctggaa gggtgaatac
     1621 accgaagaca aggtggcggc ctatcagacc ttgtatgagt gtatgacgac cgtctcaaaa
     1681 ctcatcgcac ccattgcccc attctttgcg gactggctgt atcgtaattt gaatggcggg
     1741 tccggactgg agaagcacga gtcggtacat ctagcctact tcccggctga caatgaggat
     1801 gtcatcgaca aaaacctcga gaagagaatg gaatatgcac aacgaatttc atccttgggt
     1861 ctttcacttc gtaaaaaaga gaagatccgt gtacgtcaac ctttacagaa actaattgta
     1921 ccggcactga gcttagattt caagactaag gtagaagcag taagggattt aatagaggca
     1981 gagctgaata tcaaggagtt gctggttact gccgatacgg aaggtttgat taaaaaaggc
     2041 gctaaaccta attttcagac gcttggaaaa cgactgggca agcaaatgaa acttgcacag
     2101 aatgtgattt ccaacttgtc acaggcagat atcaggcaat ttgagcaaac aaaccattat
     2161 gtactcgaaa ctccggatgg agattttgat ctgacccttg aagatgtcat catcacttcc
     2221 gaagatattc cgggttggct cgtagcagtg gacaacgaaa tcactgtcgc attggatatt
     2281 gccatcactc ctgagctaaa agcagaagga atagcacgtg aattggtcaa taggatccag
     2341 aatatacgca aaaacagcga cttcctggtt accgacagga ttaacatcaa ggtcgaacct
     2401 atagatatga caaaggaagc agtggctttg ttcgctaact acattgccaa tgaggtgttg
     2461 gcagacagca tcagtttaga ggcgaatgag ggtgaggaag tggaattgct ggatgatatc
     2521 aatatcagaa ttcaggttga aaaagcctga taggttatac caggtgcaat agaaatgatg
     2581 tttagagcct attaatgagc tctaaacatc attcaatcac aagatcacat cttcactaat
     2641 ctttgttgaa tcgccctgtt gtcaatcttt aaggacaaaa tatacacacc cgggtgtaat
     2701 gctccgattg caatattgtt ttcaccggaa ttaaggatct ttccggattg catcttcctc
     2761 ccatgcacat cgcttatttg atagttgccc gattcaaaag caagtgcggt cttcaggctg
     2821 attgtttcag tcgcagggtt tggaaagatg gatacaccgg tatcaccgaa gccatagttg
     2881 gccgaatgtt caacattaac cgtcaaggtg acactctcag tacaaccgaa gccgttggta
     2941 taagtaagtg tcacagtata agtacccggc gcagaaaata catgatccgg tttgacatcg
     3001 ctggatgtgc tgccatctcc aaaatcccat ttgtactcgc cttccagatc aatatttggc
     3061 gtgaaatgcc attgcagacc cgaatcattg gtagcattaa tagataggct aatatccgga
     3121 cttaacgcac ggattgcatg atccatccat tcaaatcttt gatgaatcca acctttcaat
     3181 ttatctatct cgccctggta agtagttgga atcggatctg tattaggcca tacccatatg
     3241 ccaagaatgg gccaacgatc aaaattgtga ttctctgcac cctgcatctc cgctacaaaa
     3301 ccatcgatga tcgaagctat atgatcttcc gacaatgttg tctggcgcaa ggactcccag
     3361 cggcaacgca tgtttctggc aaaggtagtg tccgtaagta tctttgacca ccagaatggt
     3421 gagcgaccat ccggacagat aaagttgata ttgaacaccc agccatccgg cctggcagtc
     3481 tcacaataat tggcattaaa ccatgctatg tcaaagtccc ataccggacc gaaatgtatc
     3541 ttattatctg catccgccct cgtcttatac atgaaagtac taagccgata tgcgtcaaca
     3601 ttcttggaca tctcctggat cagcatgaag tccatgaagg aaagttcatc tgcatacttg
     3661 cgccatccat tcaccggatt ctgaaaatca tcactgtgca aggctgtttc aaagctatcc
     3721 acaaaggact cgatataagc tttttgggca ttatttattt tatcatcctt gggatattca
     3781 aaaaggaaat tggtatactc aaaaggtctc ccgggtattt caaactttga tgtccagccg
     3841 tccgtgccac tgccattgat ccggtcaatc ttgaagatat aaccccccgt aagtgccatt
     3901 ccagatgtat cattttccga taacttgggg atatcaaccc ggttcttatc ccgtttgatt
     3961 ttttccataa aggtatatac gccccgatat tcaccattga taaacaattc aaccaattgt
     4021 gtccgggagg cataatgccc catttcactg aaaagcttgt aagtcatatt gttccggaag
     4081 agcgttttat ctgtataatg agcatttagc acccaatcgc tttctttagg catacccagt
     4141 actgatgcct taatatcctc tccattttca tcccaaagtt ccaatccgaa agatttctta
     4201 ggaaatgact gggaactgga acctctgatc tcaataccaa tattgcccga ataggaataa
     4261 acggtatcat tgacataatt gagggcaccg ttgtcattgg aaattatctt aaaagacgcc
     4321 ggaatcttag gatcatcagg aatggactga ccgtaagtat ctattttgat gattggcagt
     4381 gcactgcttt caaacagata gggtttggac ggatttgagc caaactggat ctcccaacta
     4441 ttcaattttc cgggatcttc aggatagacg tccaaaatca gaagtttcca ggtgccattc
     4501 gggttgcttc catcattaaa aagccccata tcaccactag gtctgtaagt tcccgaaaac
     4561 gggccccacc catggaaaat gtcattcttg ttacttccgc tgaggcatgt attcttaaaa
     4621 tcatctgaat ctccacctgc accttcaatc agggtcacga tcctgccgga cggtgattga
     4681 agctttatta tcaggtccga atcccaggta tgggaaatat ccacacatac ctgttcaatg
     4741 ccaaatgtaa ctgaattcat ggaagagggc aaaccactca cttcacaggt aaaagtagtg
     4801 gttgttacat catccgcaat acttcctgtg gtaccatgga atgcctgacc tgcaacccag
     4861 ccatacatag ctataatggc caggaatgta taaaaatatc tcatttgcaa tttaatttaa
     4921 tatttattga attgtaaaag aactacttag tattgaatgc ttagctgtta atttgataaa
     4981 ataagcgccc ggagcaattt gaagattttt tctaagcaaa tggttgagtt tgtcgacatt
     5041 acccttaccc gacaacacta ccctccccct attgtcatat atttccatac gataaatctc
     5101 aatggcctcc cctgtctcaa cctgaaccaa acgtccatca gaggttggat tggggaaaac
     5161 tttaagatgt aaagaaacgt tcttccccac agcattgctt tgctcttttg ggtaaaccaa
     5221 aattttcttc ccatcatagc ctatggcata aatcttatgg ctttggtcgg cataaagtac
     5281 ggaggtagtc ttgttggaca cgtcggcagt accggtgatg gtcacatgaa tcagtgtgtt
     5341 ggtactgtca taagtctcat aagcaactgc attcttccat tcattatggt caatgagcag
     5401 ataaggggta tcttcaacaa taatcaaatc cacaccttca ccggtccggc ctgaaaggga
     5461 caacttgttt ttatacattt ccatgctgtg ggcagaaata taatgcaaga ccggagatac
     5521 aggcttgttg gggaaagaag ccctcaactg agctaccagg gcatcaattt gattttgtgt
     5581 aaccgtaatc cgtccgaccc catagtcgtc aatctcttta tcgataggcc tcagaaatcc
     5641 actattgata aagaaatcgg acaaatcctc ctgaaccgcc tcacaggtat attttacaaa
     5701 attgagcaac ctttcaccat tggttaggct ctctgagttt gtgttacgta ctttctcagc
     5761 aacaatacca taccaatgtg gataatcgac tccgtgcaca tcggggtttt cttcatacgt
     5821 caactccggt gcgttgcgac aggcacctgc caattggtaa tataactgca attgccagaa
     5881 tggaaccaac accctgaaat gataatcatt ggctacatcc tgaagtgcct ttccatgaat
     5941 ataattggaa tccaggtaga tattgaacct gtttcctgcc caccatttag ggtcgtttcc
     6001 attccggaag gcctcctcct ccagcctggt aaaacgcttg ccctccttat ttagcttata
     6061 ggcagcccat agcgcataca cattgttggt tacttccgta gtccctatcc accttaatcc
     6121 cggccggatt tgattgatat ggccaaattc gtgtggtatg ccccatagtt ccagctgacg
     6181 ggcattggtt atgctggcgg ggccccaggt cagatcgaga tggattccga tgccgccggc
     6241 gtaccatcct cctcctgtac cactttcaca gaggatatga ttgtcatagt tgtagttgaa
     6301 tttatacaat cccatctgaa ttttttgcca atttgcaatg gtatcataca actgaatcag
     6361 atgattgccg tcaaaaggtg aattctgacg caatgcactt ttatcaaaaa ccaaatgagc
     6421 atatcggcct actatatcga tctttggata tgctgaatta gcaagaaaag tcgtccaatc
     6481 attggaatta tgaagaatcg gatcgtaata accgttgatc ttaccggtag ctatatgaac
     6541 cgtaatggcc ggtacctcag cgggcttatc agaaaaatag gctaaatatg caagaccact
     6601 atccttcatc gcaatcacat ttcggccatc attcaacagg taaagataat cggcaggatt
     6661 actctcattg gcaaaatttc tcactctcaa atatactgac gagccaccaa ttccatccgc
     6721 aaaaattact gctttatcac ctgcctcaaa tagaataccg gtcgcatttt catacggatc
     6781 ataccgatca gttttcaatt tatcgcttaa ttgtccggtg gattcaaagg ccttaaaatc
     6841 tctgatccga agtgaccgat tgtaggtgcc ggcataaaga catgatgcca gttgcttgta
     6901 gaatggaaca ctgatggtat ctatcatttc ttgagtgacc aagggaagaa ggtcggtata
     6961 taacctgtcc gtaaagattt gttcatactc aaatacatta ggatctccac catttttttc
     7021 ataaaattcc atctcagcac aactggcaaa gccacttgcc ccatttttaa cgactatctt
     7081 gacattattg actttcaagg aagtcggaaa agcgatcctg accggcaggc cttccatttt
     7141 gaaatcataa tccagcaaat gcgtgtattc agcaccaggc ccggactctt ccgtattgta
     7201 ataaatggaa gtagcaccaa aaaaaccatt gtagcctcca tcacttcttg gataataaat
     7261 gaaataatct atcacgggct tattgctaaa atgataaacg agatcaatag gaaaagcatc
     7321 ctgacctgca ttataatttg aatggtataa ggtcgaaaaa ttaccatcaa aactcttttc
     7381 aatattctca taatattggt atgatgatgc tgaagatccc gcttcgtcta tttttatttt
     7441 ggcatcctta ttaccctgga gagtggaagc atccagactg caatcaatgc tcccgtcagg
     7501 caatgcaggc ttagtcccgt agaatctcat ttccgcacaa ctggaataat catcataggc
     7561 ttcatgaaca gctatcctga tcgcaaagac gtgctccagg gcggagtcaa gattcacgga
     7621 cttgacatca ttattcaaat cccagataat cggatcggaa ttcaatctga tgaattgatc
     7681 cggcttatct ctttttgaat acatgatgtc taccttagtc caaataccat tatatctctc
     7741 ttttctcggg ctgtattcta ttttattgat ctcaggaaca ttaggattaa agtaaaaggt
     7801 caatgtgtcc ggcaccgcat agaggtcata cctcgaatga tagagggtgg catcattacc
     7861 atcaatggca ttgcctacct cttcgccacc ttgaccgggc ctgtaggaag ttgcgtaata
     7921 taccggcatt tggtactgac catacacata accaaatgta acacaaagaa ttaatgaaat
     7981 ataaacaaat ctgatcatct tgaaaacagt aagcttcaaa ggtattcaat tggcagaaga
     8041 aattatatta ttttgtgcag aatcggattt tttggatgca ctcctaataa tattgtgaaa
     8101 ttttctcaaa cagcttcatc catcaccaaa ggtaagttgg tcactatcac cgacacttta
     8161 ttgcattatt gttcgatctg tgtctttcaa tgaaggagag ggcaatcaga ttaataccga
     8221 ggacagtatc gtggttgcaa aaaaatattc cggaatgaat tgcttatcaa attatacagc
     8281 ttttgcccct caaacaaaat tagtttgtta taaaaaattt gatttgccta tagtccggat
     8341 caggaatctg aatgcaatta ccggatcata ttcttcatcc agaaatcacg cattggtttc
     8401 agaaagcctt ctctcatttc ctgccaactg gtcaccaaag ggtcgaaaaa caaggtgtcg
     8461 tcaagaatga ttccatcccg gtaggccgac ttcatatctt ttctacggat caaaaccggt
     8521 ttgtgatcct tcagatagac aaaagattgt ctgtcaagaa gatcgacatc aaagtctatc
     8581 tgaagtgttt gaataaatcg catagcagta tcaatagaac aaccgcttgc caaatacatt
     8641 ttattatcaa cggcgagaat gacgaaatat cggtgtaaaa cgaaggcccg tgcactcaat
     8701 ggatcaccat gactcaccca ggattgtgca aattcttcca gtcgggaatt gatttgggta
     8761 atttccgtat cggacaatac gcgattggcg gtaaaaaccc aaaccttgct gtccggatta
     8821 aatttttcaa gttccttcaa tgaagacata ttgattcgtt atggattaga tggccattcc
     8881 gccgcataca ttgagcacct ggccggtgat gtaggatgaa aggtcagaag ccaggaacat
     8941 agtcacattt gcgatttctt cagcttcccc aaggcgtttc aacggaacat tggccataaa
     9001 accttctttt acgctttcgt ccagcacctg cgtcatttcg gtagctatat aaccgggtgc
     9061 aatggcatta catctgatat tcttactgcc caattcctta gcaactgact ttgtaaaacc
     9121 gatgataccg gcttttgagg cggagtaatt ggcctgtccg gcattgccaa acacacctac
     9181 tacagagctc atattgatga tactgccacc tgtacgcaac ataggccgga tggcgtgttt
     9241 ggtcaggttg aacacggatt taaggttagt atcaatgaca gcatcccaca tctcttctga
     9301 catcctgagt aaaaggccat ctttggttat tccggcatta ttgaccagga catcgatctt
     9361 tccaaaatca cctaatacat ccttgacaag ttgttccgca gccgacattg atgcagcatc
     9421 cgactgatat gccttgattt tggtctttgc agacaattca gcaaccaatg cctcggacgg
     9481 gcctgcagat gaagcatagg taaatgcaat ctccgcacca tgctcagcaa aaagtctgac
     9541 aatcgctgct ccgataccgc gggaaccacc cgttataatg gctgtttttc cttccaataa
     9601 tttcatgtta ctgtaaaata aattttccgt ttgataataa ctttttttcc gggccaatca
     9661 tttggtaaat ataagtgccc ggtatatgac ctgaggtatt tatttcgata aaacttcttc
     9721 ctgcatccgg ttttataacc gatcggagat tgccctgcat gtcaaatatt ttgatttcaa
     9781 tgtctcgtgg tgcagtgtcc gcccaactta tccggactgc
//
LOCUS       DAOQQN010000069         9590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2652_length_9590_cov_5.413739, whole genome shotgun sequence.
ACCESSION   DAOQQN010000069 DAOQQN010000000
VERSION     DAOQQN010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9590
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2652_length_9590_cov_5.413739"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..723)
                     /locus_tag="PLG24_03995"
     CDS             complement(25..723)
                     /locus_tag="PLG24_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24079.1"
                     /translation="MTLIAKPFHHLFFIIAVTQSILLTSCGSYSSMQTAKTVETGTGE
                     IGLNLYYPYGVINAIAQVADEDKKNFTIPYIQATGKYGIMDRLDAGINLSTFGQIGLE
                     AKYQFYGDQQSIFAAATGLGFNTFFFYYYDFQIPLHLSVHPLENLGIYATPKYIGQFV
                     NSFGLANTYFDYGGISGGFIYGNRVQFCVDITAAFPLRKIYSIDVFPNLYNFGAGVKW
                     KIGGKTNKRSPYRF"
     gene            941..1468
                     /locus_tag="PLG24_04000"
     CDS             941..1468
                     /locus_tag="PLG24_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24080.1"
                     /translation="MRKLVFLLMVVATGLNAQDGIKTFRHEIKINTLNLLLGNPDLSY
                     EYLPDSRSGVGFFANINVDKNRLWDYDYMLGPYYRFYTGKKHASGFFIEGGVVVFGER
                     VVTGYYYDPETFTGHDIYVKEIGFGPSLAIGGKFIIAERTVFEFFGGLGRNFRGTEHG
                     SDIFSRFGISFGKRF"
     gene            2208..3095
                     /gene="tatC"
                     /locus_tag="PLG24_04005"
     CDS             2208..3095
                     /gene="tatC"
                     /locus_tag="PLG24_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HMZ24081.1"
                     /translation="MPLDQVDVDNMNTEEKEMTFFEHLEALRWHIIRSLIAVLVIATY
                     LFMQKDFLFKTIVYGPRDPGFFTYRLFCSFGEMMCIQPTHFDVITRDLAEKFTTHITV
                     SMVLGLIVAFPYVFWEFWRFVKPGLYPKERKSARGIVLVCSILFIAGVLFGYYFIAPF
                     ALSFLAGYELADTLATPTLNSYINYMIMFTLPVGIIFEMPVVSFFLTKAGVLSPGFLR
                     KGRRYALIIILIVAAILTPSPDVVSQLILATPLYILYEASIGVSARVYKGIEKRQKIE
                     EAELEAREKALLQKGRPLE"
     gene            3130..3567
                     /locus_tag="PLG24_04010"
     CDS             3130..3567
                     /locus_tag="PLG24_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24082.1"
                     /translation="MERYSSNLTLFYVVFLPVFSWTFLIFGVVGVFVGPIVNDDIYNS
                     IYIKMAFVVLLVVISIINIFFSLRLKRIEADDNRIYISNYFKHTTMSLDNIKSIRIRD
                     LWVMRIVRIRFHNKSMWGQSVLVLERENLLEKFCHAHGIAVES"
     gene            complement(3586..4698)
                     /locus_tag="PLG24_04015"
     CDS             complement(3586..4698)
                     /locus_tag="PLG24_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ24083.1"
                     /translation="MAQPRYRNIYVAATNQHIGKTTSTLGLVAAFLNRGLNVGYCKPV
                     GQQSLNFHDLIVDKDTILFADLIRFPIRQEVHSPVILGTGATTEFIDFPEKYNYPEKI
                     RYAQGILQEEHELTIYEGTGHPGVGSIVNLSNADVASMVNAGVIMVVDGGIGKTIDRL
                     NMSLSLFREKKVDLIGVIVNKVEETKIDKIRHYLGKAMSRMDLPLLGLMPYDKTLGYP
                     IMKSIMDAIKGEVLYNSDMLNNKVEDILAGSLMDVEQLKSDKGLLLVVSTGRVNEAIN
                     KLELISKIAGIRVSPLAGIVATGEGNINAKSIKYIEKNRIPLISTSLDTYGSVIKISR
                     IEVKINLNTPWKVQRAIELISNNVDLDAILDYATGK"
     gene            4884..5237
                     /locus_tag="PLG24_04020"
     CDS             4884..5237
                     /locus_tag="PLG24_04020"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HMZ24084.1"
                     /translation="MDQPFRKQDSVYSNKLRAGKKRTYFFDVKQTKGNDYYLTLTEST
                     RKFNSDSQERHKILVYKEDFNRFLAALQDTIDHIKTNLMPDYDFDEFARHQEELEKEY
                     EKRRASESTEDDMNW"
     gene            5387..5893
                     /gene="ribH"
                     /locus_tag="PLG24_04025"
     CDS             5387..5893
                     /gene="ribH"
                     /locus_tag="PLG24_04025"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HMZ24085.1"
                     /translation="MAKHTPLELPDLSEFVTEIKLGIVDTEWNPEIIRSMKVSARNTL
                     TEAGLSEDQWTELTVPGAFELPMGAKILLENGKYDAIICLGCVIKGETSHDQHINRSV
                     SSALMQLGLMSDIPIIFGVLTPNTEEQARERAGGSKGDKGREAAITALKMISLKFSFK
                     KHKKKIGY"
     gene            6198..8048
                     /locus_tag="PLG24_04030"
     CDS             6198..8048
                     /locus_tag="PLG24_04030"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMZ24086.1"
                     /translation="MKLIMSPLKFSLLALFCSFYFCVGAQQCYWQQHADYKIRVTLDA
                     SKHTLAGVEDLVYTNNSPDTLYEVFYHLYFNAFQPNSQMDVRSRTITDPDGRVMDRIS
                     QLKPHEQGEYKIRGVVQDKDKVKGVTVDGTILKVTLNKPIYPGEKTRLIMDYECQVPV
                     QIRRTGRYNKEGVAYSMSQWYPKMAEYDRNGWATDPYVGREFYGVWGRFEVFLTVPDS
                     FKIAGTGTPVSDTKSRKEKASVPSEYTMHHYVAENVHDFMWAADPHYRIDRITAAPGL
                     DLVFAYKDNPKVAETWKALQPIMKEAINYASERFGKYPYSRFSFIQGGDGGMEYPMAT
                     LVMGELSLNSLISVCVHEMMHSWYQGVLGFNESLYAWMDEGFTSYSEELVKEHLRSKG
                     MYPGKVSANPFERDAISMINFTLAGKSEPLSTHADHFTTNAAYSVASYVKGSLFLSQL
                     GYVIGEDALRSGLLEFYNIWMFKHPTGQDFMNLMEHRTGMELDWYYQYMINTTMLPDY
                     AVDNVKADGTGTAIMLGKKTQMPMPVDVTVEMKDGQKRFFTIPVDLMRMAKKTDQAMP
                     GLTPLKAWNWTNPDYFFHLDIPFAEIHSITIDDSNRMLDSDRKNNKWANE"
     gene            8144..9004
                     /locus_tag="PLG24_04035"
     CDS             8144..9004
                     /locus_tag="PLG24_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HMZ24087.1"
                     /translation="MRKVVSIVYPALLLLFLTSCKTSSAVSSYGSGTAVADTHVGAVS
                     NRKVHDVALNGKVYSAVWQQNAGEFKALCYQAYNAARQMVDQEKAKPHSKPLAIITDV
                     DETILDNSPYAVRQAELGNEFDIKSWTEWTAKGQAVAYPGSIAFFNHAAIRNVEVFYI
                     TNRAEAERDGTLKNLRVLGFPYADEAHLLLMKETSDKEARRQEVLKNYEVIMYIGDNL
                     NDFSKVFYKLSQEERNKKTDDLSREFGSRFIVLPNSGYGNWESAMPGYKSGMTPEEKD
                     AIFMKNLQKY"
     gene            complement(9050..>9590)
                     /locus_tag="PLG24_04040"
     CDS             complement(9050..>9590)
                     /locus_tag="PLG24_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HMZ24088.1"
                     /translation="IDLLKSLDQPPLVAFCTAYPNFAVEGFELDALDYLLKPVSMERF
                     NKAINKAREELSHKTDIKSETKSSSNPDYIFVKADKKLMKINYDDILYIEGLKDYVII
                     RTEQSRIITLQTMKSLEEKMPLDKFIRIHRSFIVSIDKINAIVGNMVEVIEKNQSKLL
                     PIGKNYRDELSDMINQSKL"
BASE COUNT         2769 a         1714 c         2187 g         2920 t
ORIGIN      
        1 aggtacatat ggttttcctt ttgatcagaa cctgtatgga gaccttttgt tagttttacc
       61 gccgatcttc cacttgacac cggctccgaa gttgtataga tttggaaaaa catcgatgga
      121 atatatcttg cgaaggggaa aggctgctgt tatgtcaacg caaaattgta cgcggttacc
      181 gtaaataaac cctccggata tgcccccata atcgaagtag gtgtttgcaa gtccgaaaga
      241 gttgacaaac tggcctatat attttggggt tgcatagatg cccaggttct ctaaagggtg
      301 taccgatagg tgaagaggaa tctggaaatc ataataataa aagaaaaaag tattgaagcc
      361 aaggcctgtt gctgcggcaa agattgattg ctgatctccg taaaactgat atttggcttc
      421 cagcccaatt tgaccgaagg tactcagatt tatacctgca tccaacctat ccatgatgcc
      481 atactttccc gttgcttgta tataaggaat cgtgaaattt tttttgtcct catcggcaac
      541 ctgagcaatg gcgtttatta ctccgtaagg atagtataga ttgagcccaa tttcgcctgt
      601 ccctgtttcg actgttttgg ctgtctgcat gctgctgtat gatccgcacg aagtcagaag
      661 gatggattgg gtgaccgcaa taataaagaa taggtggtga aaaggtttgg ctattaaagt
      721 catggtaatt atcttattag aattttgtat caaaagtata acgaaatatg aatagtcagg
      781 aaaggttaaa taaaacctaa aagaaaataa aaattatctg gtttaggtta ttcaatggaa
      841 gatacaataa cataggcttg aaagaggaag ctactaattt gatgaatatt gaatttttgg
      901 ggtaaaataa tattatcttt atataacaaa aatagaatgt atgcgaaaac tggtatttct
      961 tttaatggtg gttgctaccg gattaaatgc acaggacggg attaaaacat tccgccatga
     1021 gattaaaatc aacacactga atctattgtt gggcaatccg gatttgtcgt acgaatatct
     1081 gccggatagt aggtcgggtg ttggattttt tgcaaatatc aatgtagata aaaatcgttt
     1141 gtgggattat gattacatgt tgggtcccta ttatcggttt tataccggaa agaaacatgc
     1201 gtccgggttt tttattgaag ggggtgtggt tgtattcggc gaacgggtag ttaccggata
     1261 ttactatgat ccggagacat ttaccgggca tgatatttat gttaaggaaa ttggctttgg
     1321 gccttctttg gcaattggtg ggaagtttat aatagcagag aggactgtat ttgaattttt
     1381 tggagggtta ggcaggaact ttagaggaac agaacatgga tcagatattt tcagccggtt
     1441 tggaatctct ttcggcaagc gtttttaata taaaaatgcc ggaagatgat tggcctttgg
     1501 tgtgttgacg agatcgatgg attaaaaagt cagtacaccg ggttggattg ttaactggac
     1561 aaatcgaatt ggtaaaattg attaatagct ataagtctat tgatactaac ttaggtacac
     1621 attactatat ttataatgtg tataaaatgt ttagaaaagt gttaatgagt tgcctgaaaa
     1681 taaataaaag aattatcttt gtgtaaaagt tacacaaggt gacttgaaag ccaattgatg
     1741 tattggtagc aaataaaaaa gaaaaaataa tttttattat ttgaaacttt tattatattt
     1801 gcaatgttat aagtatgact tgtgagtttg caagttgctt ttcaaaaaag tatgaaaagt
     1861 tttccggcgg gaatagaaac acgccacaaa cacggggtga tttgaagttt tcataattgg
     1921 gccctgtatt catctctgag tacagggttt tttttagaaa catcacccgg tggagattcc
     1981 ggcgggaata gaaacacgcc acaaacatgg ggtgatttga agttttcata attgggccct
     2041 gtattcatct ctgagtacag ggttttttta ttgccagatt ttttaataag atgattctat
     2101 tgaatttccc cgataaatat tgtcttttgc atgtttttca aattatttgc aattagggaa
     2161 tgcgcaaaac tttacctttg ccaggttgat aaataaagga attgaatatg ccattagatc
     2221 aggttgatgt agataacatg aataccgaag agaaggagat gacttttttt gagcatcttg
     2281 aagccttgag atggcatatt atccggtcct tgattgcagt acttgttatt gccacatatc
     2341 tcttcatgca gaaggacttc ctgttcaaga ccattgtgta tggaccccgg gatcccggtt
     2401 tttttaccta tcgcctgttt tgctcgtttg gggaaatgat gtgtatccag cctacccatt
     2461 ttgatgttat cactcgggat ctggcggaaa agtttaccac gcatattacg gtttccatgg
     2521 tacttggact tattgtggct tttccttatg ttttctggga gttttggcga tttgtcaaac
     2581 cgggactgta tcccaaggag agaaaatctg cccgcggcat cgtattggta tgttctatcc
     2641 tgtttattgc cggggtttta tttggttatt attttattgc cccttttgct ctttcttttc
     2701 ttgccggata tgaattggcg gatacgcttg ccacgccaac cctcaattcc tatatcaact
     2761 atatgatcat gtttacccta cctgtaggaa ttatttttga aatgcctgta gtttcttttt
     2821 ttcttacaaa agcgggtgtt ttatcgcccg gatttctaag aaagggcagg aggtatgccc
     2881 tgattatcat attgattgtc gctgccatcc tcactccatc gccagatgtg gtctcgcagt
     2941 tgatccttgc cacacccctc tatattcttt atgaggctag tataggtgtt tcggctcgtg
     3001 tgtacaaggg gattgaaaaa agacaaaaaa tcgaagaagc cgagctggaa gcgcgagaaa
     3061 aagcattgct tcaaaaaggc aggccattag aatagtgctt cttttcctta cctttgcaaa
     3121 aatctttcta tggagcgata ctcttccaat cttactttgt tttatgttgt ttttttaccg
     3181 gttttttcgt ggacattctt gatatttggc gttgttggag tttttgtagg tccaatagtc
     3241 aatgatgata tatataattc aatatacatc aaaatggcct ttgttgtact attggtcgtc
     3301 atctcgatca tcaatatttt cttttccttg aggttaaaaa ggatagaagc ggatgataac
     3361 aggatttata tatccaacta tttcaaacat acgactatgt ctttggataa cataaagagc
     3421 atccggatca gggatctttg ggtgatgagg attgtccgta tccgatttca taataagagc
     3481 atgtggggtc agtctgtcct tgtactggaa agagagaatt tattggagaa gttttgtcat
     3541 gctcatggta tagccgttga aagctaagtc ctgaatcagg cttgattatt tcccggttgc
     3601 ataatccaga atcgcatcaa ggtcgacgtt attgctgatt agttctatcg cccgctgtac
     3661 tttccatggt gtgttcaggt tgattttgac ttcgattctt gagatcttta ttacactgcc
     3721 gtaggtatca agcgaggtac ttataagagg gattctgttt ttttcaatgt atttgatact
     3781 cttggcattg atatttcctt cgcctgttgc aacaattccc gccaggggag atacgcggat
     3841 gccggcaatc ttactgatta gctccagttt gttgatggct tcattgaccc ggcctgtact
     3901 cacgacaagg aggaggcctt tgtcggattt caattgctcc acatccatga gggatcctgc
     3961 taatatatct tcgactttat tgttgagcat gtcactattg tacaatacct cgcccttgat
     4021 ggcatccata atagacttca taatcggata tcccagggtt ttatcataag gcatcagacc
     4081 aagcaacgga aggtccattc tggacatggc tttacccagg taatgtctta ttttatcaat
     4141 ttttgtttct tccaccttat tgacaatcac accaatcaaa tctacttttt tctcccggaa
     4201 tagtgaaagg gacatattga gccggtcgat ggttttgcca attcctccat ctacaaccat
     4261 gatgacacct gcattgacca tggaagcaac gtcggcgttg ctcagattga caatactccc
     4321 tacacccgga tgcccggtgc cttcatagat ggtcaactcg tgctcttctt gcagtattcc
     4381 ctgagcatac ctgatttttt cagggtagtt gtatttttca gggaaatcaa taaactccgt
     4441 ggtcgctccg gtacccagaa tgaccgggct gtggacttcc tgtcggattg gaaaacgaat
     4501 caaatcagca aataagatag tatccttatc cacgatcagg tcgtgaaagt tgagtgattg
     4561 ttgtcctacg ggcttacaat atccgacatt caaacctcgg ttgaggaatg cagctaccag
     4621 ccccagggtg gatgttgtct tgccgatatg ctggttggtc gcggcgacat agatatttct
     4681 gtaacggggc tgtgccatac ttttttttct acaagtatat aaatttcaat tgaaatcgac
     4741 aagatacttt ttacagatac ggaggctgaa aaaaggcaat ctgcaattta tacttttaaa
     4801 aaataatgaa ttattaaaaa gataatttat aatcaaataa ttatatattt gcttgaatta
     4861 tagaattatt aaaattaaat acagtggatc aaccatttag aaaacaagac agtgtctatt
     4921 ccaataaact cagagcgggc aagaaaagga catatttttt tgatgtcaag caaacgaagg
     4981 gaaatgatta ttacctgaca ctgacggaaa gtaccagaaa attcaactcc gattctcagg
     5041 aaagacataa aatcctcgtt tataaggaag acttcaacag atttttggct gccttgcagg
     5101 ataccattga tcacatcaag acaaatctta tgccggatta tgactttgat gaatttgcaa
     5161 gacaccagga ggaattggaa aaagaatatg aaaaacgaag ggcaagcgaa tccacggaag
     5221 atgatatgaa ctggtaagca agtgctccgg tattattgtt gctttttctc cttactcgtt
     5281 taaccggaca ccttgaacct gagggattgg aggaaattag taattgtaat tgaactaacc
     5341 ggcctattgt tcattatcag gataaatcaa tcgtataaat gtaatcatgg cgaaacatac
     5401 accactggaa ttgcccgatt tatctgaatt tgttacagaa attaagcttg gaattgtaga
     5461 tacggaatgg aatcctgaga ttatacgttc catgaaggta agcgccagga acacattgac
     5521 ggaagcaggt ctgagtgagg atcagtggac cgaactaaca gtgcccggag cttttgaatt
     5581 gcctatgggt gcaaagatcc tgctggaaaa tggaaagtat gatgccatca tttgtctggg
     5641 ctgtgtcatc aaaggtgaga cgagtcatga tcaacacatc aacaggtctg tatcttctgc
     5701 tctcatgcag ttgggcttga tgagcgatat ccctatcata ttcggtgtac ttactccaaa
     5761 tactgaagaa caggcccggg aaagggcagg aggctcaaag ggcgacaagg gaagggaagc
     5821 tgctattacg gcattgaaga tgataagctt aaaattcagt tttaaaaaac acaagaaaaa
     5881 gattggatat taagtacaaa tccaaatgga tggaatgtcc ttttgagtgt cttttccggg
     5941 agttattgtc cgaaggtatt tgttttagga aggaataata caatcatgtg caatgtgaat
     6001 attgcagggg agacgataaa aatccccgtg atgaagaaga tgggtggatg agagataaag
     6061 gatagtggcc aggtaggaaa caggtatggc gaggagtgaa acgaaaaaga gccaaataat
     6121 gattaaacct gacattgagt tagtttctat atttttgatc atcataattt aaaatttttc
     6181 caacgaaata gcttttaatg aaattaatca tgagtccatt gaaattcagt ctattagctt
     6241 tattttgttc tttttatttt tgcgttgggg cgcagcaatg ttattggcag caacatgcag
     6301 attataagat acgtgttacg ctggatgcga gtaagcacac cttggccggg gttgaagatt
     6361 tagtgtacac caataattct ccggatactc tgtatgaagt gttctatcat ctttatttta
     6421 atgccttcca gcctaacagt caaatggatg ttcgtagcag aaccattacc gacccggacg
     6481 gtagggtcat ggacaggatt tcacaactaa aacctcacga acagggtgaa tacaagattc
     6541 ggggagtggt gcaggataag gataaggtaa aaggcgttac ggtggatggg acgattttga
     6601 aagtaacctt gaataagccg atttatcccg gtgagaagac gaggttgatt atggactatg
     6661 aatgtcaggt tcctgtgcag atcaggcgaa ctggaaggta caacaaggag ggtgtggctt
     6721 attccatgtc ccaatggtat cccaagatgg cagaatatga tagaaatgga tgggcaactg
     6781 atccttatgt aggaagggag ttttatggag tctggggtag atttgaagtt tttcttactg
     6841 tccctgattc atttaagatt gccggtacag gtacaccggt gtctgatact aaaagcagga
     6901 aagagaaagc atctgttcct tccgaatata ccatgcatca ctacgtggct gaaaatgtcc
     6961 acgattttat gtgggctgct gatcctcatt accggatcga taggataacg gcagctcccg
     7021 gacttgattt ggtatttgcc tataaggata atccaaaggt ggccgaaaca tggaaggcac
     7081 tgcagcctat aatgaaggaa gccatcaact atgcatcaga aaggtttggt aagtatccat
     7141 attcccgctt ttccttcata caaggaggtg acggggggat ggaataccca atggcaacac
     7201 tggtcatggg tgagctgagt ctcaatagtc tgatcagcgt atgtgtgcat gagatgatgc
     7261 attcctggta tcagggcgtg ctgggtttca atgagtccct atacgcctgg atggatgaag
     7321 gttttacttc gtattcagag gagttggtca aggaacatct gcgatccaaa ggaatgtatc
     7381 ccggaaaggt ttctgccaat ccatttgaaa gggatgcgat atcgatgatc aactttacct
     7441 tggcaggtaa gtcagagccg ctttcgacgc atgccgatca ttttaccacc aatgctgcct
     7501 attcggtggc atcttatgtc aagggatcct tgttcttgtc tcaacttggg tatgtaatag
     7561 gggaagatgc attaaggtct ggcttgttgg aattctacaa catatggatg tttaaacacc
     7621 caaccggtca ggatttcatg aatttgatgg agcatcgtac cgggatggaa ttggactggt
     7681 attatcaata tatgatcaac acgacaatgt tgcccgacta tgctgtggac aatgtgaagg
     7741 cagatggtac agggacagcg ataatgcttg gaaagaaaac gcagatgcct atgccggtgg
     7801 atgtaacagt agagatgaag gatggtcaaa aacggttttt cacaattccg gtagacttga
     7861 tgaggatggc taagaagacg gaccaggcta tgcccggact tacacctctg aaggcctgga
     7921 actggaccaa tcctgattat ttctttcatt tggatatacc gtttgccgaa atccattcga
     7981 taactattga tgattccaac cgaatgttgg atagtgacag gaagaacaat aagtgggcaa
     8041 atgagtagaa actttgattc aaaatcctat ctttggacaa gaatcattaa ttattgattg
     8101 ttattgggag ttgatcctcc cccttaaatt aagataaacg aatatgagaa aagtagtttc
     8161 aattgtatac cctgccttgc tgcttttgtt tcttacctcc tgtaaaacct cgtcagcagt
     8221 atcttcctat ggttcaggca ctgcagtggc tgatacccat gttggtgctg tgtccaaccg
     8281 aaaggtccat gatgtagctt tgaatggtaa ggtgtattcg gctgtgtggc aacaaaatgc
     8341 cggagaattc aaggcattat gttaccaggc ttataatgca gccagacaaa tggtcgatca
     8401 ggaaaaggct aaacctcatt ccaaaccatt ggccatcatc acggatgtgg atgagacaat
     8461 cctggacaat tcgccctatg cggtccgtca ggcagagctt ggcaatgaat ttgatatcaa
     8521 atcatggacc gagtggactg ccaagggtca ggctgttgcc tatcccggat ccattgcttt
     8581 tttcaatcat gcagcgataa ggaatgtgga ggttttctat atcaccaacc gggctgaagc
     8641 agaacgggac gggaccttga aaaaccttag agtgttgggt ttcccttatg ccgatgaggc
     8701 acatctactg ttgatgaagg agacgtcaga taaggaagcc cgacgtcagg aggtgttgaa
     8761 aaattatgag gtgatcatgt acattggtga taatctgaat gatttttcaa aggtattcta
     8821 taagctttct caggaagagc gaaataaaaa gaccgatgac ttgtccagag aatttggatc
     8881 aagattcatc gtattgccta attcaggtta tggaaactgg gagtcagcca tgccgggata
     8941 caaatccggc atgacacctg aagaaaaaga tgcaatattc atgaagaatc tgcagaaata
     9001 ctgatgcccg gcttgtttaa gggcactccc tatttcaatc aggctgttgc tatagcttac
     9061 tttgattgat catatcactg agttcatccc gataattttt accgataggt aaaagtttag
     9121 attgattttt ctcaatgact tcgaccatgt tgcctacaat ggcattgatt ttatcgatag
     9181 aaacaatgaa tgagcggtgg attcggataa atttgtccaa gggcattttt tcctcaagac
     9241 ttttcattgt ttgcaatgtg atgatgcgcg attgttcggt ccttataatc acataatctt
     9301 tcaaaccttc aatatacagg atatcatcat aattgatttt catcagcttt ttatccgcct
     9361 ttacaaagat ataatccgga ttggaggagg attttgtttc tgatttaata tccgttttat
     9421 ggcttaattc ctcccttgcc ttgttgattg ccttgttgaa tctttccatg gataccggct
     9481 taagcaggta gtccagggca tccaattcga atccttccac ggcgaagttg gggtaggccg
     9541 tacaaaaagc gactaatgga ggttggtcca gtgacttcaa taaatcgata
//
LOCUS       DAOQQN010000070         9562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2672_length_9562_cov_6.125381, whole genome shotgun sequence.
ACCESSION   DAOQQN010000070 DAOQQN010000000
VERSION     DAOQQN010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9562
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2672_length_9562_cov_6.125381"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..308
                     /locus_tag="PLG24_04045"
     CDS             165..308
                     /locus_tag="PLG24_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24089.1"
                     /translation="MILPINEKLRLIDQLDDEDKKAIFRIIEGMLSKSKFKDFFQKNV
                     AAL"
     gene            complement(386..949)
                     /locus_tag="PLG24_04050"
     CDS             complement(386..949)
                     /locus_tag="PLG24_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24090.1"
                     /translation="MNGIELFKSGSNSKQIKFPSAVNDFIEYKPTLYIEFLKLVFNES
                     SDSVIFNISKVKVKDIIKPIEIVYHGDIEKRDFISIDSFFSPNNCMPYQDENGNKFVA
                     IASSEDNGRGGILVGVDSCNCDEIFKYRWEESDIMPSIPFKLSNNIFEFISEIKTMPM
                     WEKEYDRSKLFKSFTNDFYEIMNCTLS"
     gene            complement(961..1533)
                     /locus_tag="PLG24_04055"
     CDS             complement(961..1533)
                     /locus_tag="PLG24_04055"
                     /inference="COORDINATES: protein motif:HMM:NF025771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HMZ24091.1"
                     /translation="MDKVKGVKAWEKLKDLTGTRKWARRSVSLLETMSGQTDEVLENV
                     KQYYSAHTKPSGTGNPPFTHHGVDFDDLGHPDFFNSNTCPGQQYCLKTDDLVGHPSGA
                     LSTDLRPDFNLANQNLINSGKEINVMHSSGSPILIKIDGKWEGPFTWHHHQDGKTMIP
                     VKQDVHNSMSHTGGDSAIKKGIKGFFNSPF"
     gene            complement(1797..2801)
                     /locus_tag="PLG24_04060"
     CDS             complement(1797..2801)
                     /locus_tag="PLG24_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24092.1"
                     /translation="MELRRIDQKENVNKFEIAQVGLLLWQKGEIYLDDNLIFENEPNA
                     VFSFQDVLFIVTNDWKDSVCIDLNSRTENILENVRGMDIFLDSKSILVSEWNDDYTKS
                     FKQKIDLDSGNIIWEVEAYFGKPFLGNGCLFASLDSKLFKIDPETGNFIWELNLADKH
                     NSYSINGQDTPIDIQGYIGMHIDCLYVKAGNRLIIGIDSSSGEEIFCYEYNGVSVLLD
                     NLRLDCDKGVIFSIGSAEYFELNLSSHKSEIVTLKVNDIETTRMGTWEDNIIYFWEGG
                     TSSNFGLFDRDTKEVRLVQTLNVSGYPAIKDIKHGHGKVYVLDGNNQLHIFEQLDYPA
                     "
     gene            complement(2807..3643)
                     /locus_tag="PLG24_04065"
     CDS             complement(2807..3643)
                     /locus_tag="PLG24_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24093.1"
                     /translation="MDKVERVKAWDFVRGATGSNSAKRLSVDILECVSEHLDDANFMT
                     IIGNGNIQSGKNAFGELINKYAGKCQACTGGNPSQYLPDNPQDYIDKVVKLTKRYSDK
                     NGVDIFNEAKKGFTSQEGAWHAMQCIDGLDPSTVKRIDANFEGEGLPCEGCRFDVELI
                     PTSSNPLKLIEYKSYLDASKIPLEQFKNYLNDVTNLNQLNYIFSRSKLDLAGAKNGIK
                     NFLNNNADDLFKAPANGGIGLDKMKQLFETGGVTFNNPTQFKAALTNNATFRNHATSF
                     VK"
     gene            complement(4028..4666)
                     /locus_tag="PLG24_04070"
     CDS             complement(4028..4666)
                     /locus_tag="PLG24_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24094.1"
                     /translation="MDKQQLIRSIRDYLELSDFKPIGVGAGNVSLERNNERISISYRK
                     YPGVYMLPGVIGGFKSFPEVENILKKYYDKYGIELQNDTIYTSSRRLDEITEIDIAEP
                     DDIFKVLPQLKVMVFDDILPFFEKYNSILSVNKKIEELQIDEYSKLVFSPIHPRIMII
                     KRLSGASDWVEYSENALGVYEEQSVGKYRTSFAPINRFLPELYEELKQMEPL"
     gene            complement(4901..6025)
                     /locus_tag="PLG24_04075"
     CDS             complement(4901..6025)
                     /locus_tag="PLG24_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24095.1"
                     /translation="MNYSTLIFNSMPYNRTGILNNQVYYVGVGGSHQGWIKCLSKNDY
                     HELKHIDLWYNVSSNFEECKYFYHTRDNIIKKLDINTFQISTFPGHHFPKYKWEDILC
                     YHNLLTKQYEVFNSKGEKVLEFKYHHPIIAKFISSGILIYQNNEKENNWIKCLNPDGG
                     DEIWKVEFSWQFVRLETFDNLIILEYHAFDKIRTDKGHAGERDWYNPDRYTIVLNGDT
                     GEEIWRYSNSYHHIDYENEAVLIGNIKSFLPNGKIEKLSVVELDIYNGNVLTEIEVKP
                     PIERIPDLHFVDGVGIYYTSHDGSFGKINKADGAILWEFDLVDNKGEKRKISDWLLLG
                     NGNLVLQTLPNHPNGDFTCIFNPIENMEYSNVIDGVRLNA"
     gene            complement(6025..6768)
                     /locus_tag="PLG24_04080"
     CDS             complement(6025..6768)
                     /locus_tag="PLG24_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24096.1"
                     /translation="MERVKAWEKIAGHIQLRVDLDVLESLSKALADDYLKTLNLDQVI
                     SNKWIKDGFGCPSCKNGGKPWINELIDQLDHFKQFKEIEGYQTVLNQLLQQGKNQAIG
                     SHWVMKFVKKKNLSPTKFEDFIDEATGDFSADIIVNGKFIECKSWGGKAPGMSNVPNQ
                     LINYFNTQNSLSKFEFQFDPDRWIPTPEDLNQALKSKSDLFNTTDEVVWAKYVSMVES
                     NVDDIPIGNVDALIDQITSSSIFSKIINP"
     gene            7000..7143
                     /locus_tag="PLG24_04085"
     CDS             7000..7143
                     /locus_tag="PLG24_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24097.1"
                     /translation="MILPINEKLRLIDQLDDEDKKAIFRIIDGLLTKSKFKDFFQKNV
                     AAL"
     gene            complement(7205..7981)
                     /locus_tag="PLG24_04090"
     CDS             complement(7205..7981)
                     /locus_tag="PLG24_04090"
                     /inference="COORDINATES: protein motif:HMM:NF020501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1911 domain-containing protein"
                     /protein_id="HMZ24098.1"
                     /translation="MVRDIHRNKEHFLSYIKSRTESYEERARKLNQGEIKPDRIIPVK
                     QNMAANLIHILVSKYSLGIPIDELVDDFNPILNLIEESWVHGSRKLIGPKHKVLNQYN
                     IDAHVQLLTILSIGFLCVISKDFFIRLGKIINDDNVIDILFECILASKLNSWKMRQED
                     DDYTFKLYSNLKKAILQSEKEEAEKLVKIFLERDWIKEQKKAQMLIEPNKSWYHGAWS
                     FESAAIVAILDLDDSSFRDNQYYPKDLVDYYRSTLKGSPR"
     gene            complement(7991..>9562)
                     /locus_tag="PLG24_04095"
     CDS             complement(7991..>9562)
                     /locus_tag="PLG24_04095"
                     /inference="COORDINATES: protein motif:HMM:NF019227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HMZ24099.1"
                     /translation="GYIALDVLGLFIGITEIKAAFKVGSVIRKIAVAADVIGSSSNLA
                     IQLIDEDVIDPVLRNRIQITSAAMAGVGLAFSVIEIGKVVNDMSKTRALYKGKFEAKN
                     LQKFDEIHKYFADVCLENRGIQKEFTDEILSLSPDLKIGGQICDFECKLSTFGCFSGS
                     TEVWTETGRKEIQTINKGDKVWSYNFAKKIKELKPIAGIKNYIVAALFSLVMSSGDTL
                     WATPSHPIYTTSGYKEVAALTVGDTLWSYSGDYERVIGKTLIDTTLQVYDLTIGDNHN
                     YYVGGAGVLVHNNGECFLKNLGNKYNSLATRIDALDEAGKSQFLLDFFDASDDMLRML
                     DGNPGMVKAWEKFVDDGLPPAFRRNISNLNQRTILDEWAENIANATNKQKGNFGEIGA
                     DLDLNAKGYTSLQPRIDNIDSPGHNGIDIVVKKDGQYFIVEGKYKGSASLNPADPTTG
                     LPRQMSDEWITQNNGQRLLEAVGGDQNLVDEILDAGYSRILAKVSPDGSVTYKYVSET
                     GYLNQGGGPLGDWLP"
BASE COUNT         2921 a         1959 c         1389 g         3293 t
ORIGIN      
        1 ggagcaatat taacaaataa tgcttctatg tggtgctttt ttccgggata ttttgagagt
       61 atctggtcaa aactttcctg agtcaggtta aggaggccgg aggcggtgtt ctgggggtga
      121 aattaatcac tacgaagata acttaccaaa tgaggttaac attgatgata ctccctatca
      181 atgaaaaact acgacttatt gatcaacttg atgatgaaga taaaaaagcc atcttccgta
      241 ttattgaagg tatgctctcc aaatctaaat tcaaagactt ctttcagaaa aatgtggcgg
      301 ctttataaaa ccacaaaaag gataaagatt aggtctttac ccttctcgat gcttttatcg
      361 ccagaactaa attacagggg tttgtttagg aaagagtaca attcataatc tcgtaaaaat
      421 cattagtaaa cgacttaaat aatttagatc tatcatattc tttctcccac attggcatag
      481 ttttgatttc agagataaat tcaaagatat tatttgataa cttaaatgga atagaaggca
      541 ttatatctga ttcttcccat ctgtacttaa atatctcatc acaattacaa gaatctactc
      601 ctactaatat tccacccctt ccattatcct cagaggacgc tattgctaca aatttatttc
      661 cattttcatc ttgataaggc atacaattat taggagaaaa aaaactatca attgaaatga
      721 aatctctttt ttctatgtct ccgtggtata caatttcaat aggctttata atatctttaa
      781 ctttaacctt ggagatatta aagataacag agtcagaact ttcattaaac acaagcttga
      841 gaaactcaat ataaagggtt ggtttatatt ctataaaatc attaacggca cttgggaatt
      901 taatctgctt actattagaa ccacttttga acaattcaat tccattcatt agattaaaat
      961 ttaaaatgga ctattaaaaa agcctttaat gcctttttta atggcactat caccacctgt
     1021 atgactcata ctgttgtgta catcttgttt aacagggatc atagtttttc cgtcttgatg
     1081 atgatgccaa gtaaacgggc cttcccattt accatctatt ttaataagaa ttggtgaacc
     1141 actactatgc atgacattta tttctttacc actattaatt aaattctgat tcgctaaatt
     1201 aaaatctggt ctcaaatccg ttgataaagc tccagacgga tgaccaacta aatcatccgt
     1261 tttaaggcaa tattgttgac ctggacatgt attagaatta aaaaaatcag gatgccctaa
     1321 atcatcaaaa tcaacaccgt gatgtgtaaa tggaggattt cctgttcctg aaggctttgt
     1381 atgagccgaa taatattgct ttacattttc taatacttca tcagtttggc cagacatcgt
     1441 ttcaagtaat gatacacttc ttcttgccca ttttcttgtt ccagttaaat ctttcaactt
     1501 ctcccacgcc ttcaccccct tcaccttatc catcaaataa gtcccgaagg ccacatcctc
     1561 caggtcggca gctaactttt tagcattctc cggagcaata ttaacaaata atgcttctat
     1621 gtggtgcttt tttccgggat attttgagag tatcttttag gtaaagtacg atttggaaag
     1681 tatgacaaag atgctgttaa aagacttgaa agtattcaaa acatggacac tgataccaga
     1741 gacaaactct ttgatatcag tgatacctac atcaaagatg ctaaagcctg acaggcttat
     1801 gcgggatagt caagttgctc aaaaatatgc agttgattat tcccatccaa gacatagact
     1861 tttccatggc catgcttaat atcttttatt gctgggtatc ctgacacatt caatgtttgt
     1921 actagtctta cctcttttgt atctctatca aagagaccaa aatttgagct cgtaccacct
     1981 tcccaaaagt aaattatatt atcctcccaa gtccccattc ttgtagtttc tatatcattt
     2041 acttttaaag ttacaatctc acttttatgt gaagataggt tcaactcaaa gtattccgct
     2101 gagccaatag aaaatatcac acctttatca cagtccaatc gcaaattatc caaaaggaca
     2161 cttactccat tatattcata acaaaatatt tcctcgccac tacttgaatc aataccaatt
     2221 attaatctat tcccagcctt aacatataag caatcaatat gcatacctat ataaccttga
     2281 atatcaattg gcgtatcttg accattaatt gaataggaat tatgtttgtc agctaaattt
     2341 aactcccaaa taaaatttcc agtctcagga tctattttaa ataacttaga atctaaagaa
     2401 gcaaacaaac acccattacc taaaaatggc ttcccaaaat atgcctcaac ctcccaaatg
     2461 atatttcctg agtccaaatc aattttttgc ttaaacgatt ttgtataatc atcattccat
     2521 tcactaacta aaattgattt actatcaagg aaaatatcca tccctcttac attctctaat
     2581 atattttcag ttcggctatt caaatctata cagacagaat ctttccaatc atttgtaaca
     2641 ataaataaca catcttgaaa ggaaaaaact gcattaggtt cattctcgaa aattaaattg
     2701 tcatccaaat aaatttcccc cttttgccat aataatagtc ctacttgtgc tatctcaaac
     2761 ttgtttacat tctccttttg atctattctt cttagttcca tctaattcat ttaacaaagc
     2821 ttgtcgcatg atttctaaat gtggcattat tcgttaatgc cgctttaaat tgtgtcggat
     2881 tattaaaggt tacaccccct gtttcgaaca actgcttcat tttatcaagg cctatcccac
     2941 catttgcagg agccttaaac aaatcatcgg cattattgtt taaaaaattc ttaatcccat
     3001 tctttgctcc agctaagtct aattttgatc tactgaaaat atagttgagc tgattgaggt
     3061 tcgttacatc attcaaatag ttcttaaact gttccaatgg aatcttagag gcatctaagt
     3121 aagatttata ctcgattaat tttaaaggat tagaagacgt aggaatcaat tccacatcaa
     3181 atcgacaccc ttcacaagga agcccttcgc cttcgaaatt tgcatcaatc cttttaactg
     3241 ttgacggatc caaaccatct atacactgca tagcatgcca tgcaccctct tgacttgtaa
     3301 atcccttttt ggcctcgtta aaaatatcaa caccattttt atcagagtat ctttttgtaa
     3361 gttttacaac tttatcaata taatcttgtg ggttatctgg caggtattga gaaggattac
     3421 cgccagtaca ggcttgacat tttcctgcat acttatttat taattcacca aaggcattct
     3481 tgccagattg aatattacca ttccctatta tggtcataaa attcgcatca tctaaatgct
     3541 cactaacaca ttccaaaata tctactgaca atctttttgc actattactt ccagttgcac
     3601 ctctaacaaa atcccacgcc ttaaccctct ccaccttatc catcaaataa gtcccgaaag
     3661 cctgatcttc caggtcggca gctaactttt tagcattctc cggagcaata ttaacaaata
     3721 atgcttctat gtggtgcttt tttccgtgat attttgaaag tatccggtca aaactttccg
     3781 gggtcaggtt taggtggcct ggagcgtttt tattggggtg aaattatggg atttaaggcc
     3841 gggagacgtt gattttaagg gtttccgggg tttaggatgt tttaattttt ggcatcgggg
     3901 tgatttttgc gataataatc caccagatct ctggagtaat atggcaaagt ctttagcaaa
     3961 atatcgtttt attcattttc ttttgccagg ggagagatag gttatgacat tttcttgtca
     4021 tggtatatta taagggttcc atctgtttca attcctcata taactctggg aggaatctgt
     4081 tgataggggc aaaactcgtt ctgtattttc ctacggattg ttcttcataa actcctaaag
     4141 cgttttcaga atattcaacc caatcactag cccctgacaa tcgtttgatt atcataattc
     4201 tggggtgaat cggtgaaaat acaagcttcg aatattcatc tatttgaagt tcctcaattt
     4261 ttttatttac agatagaatg gaattatatt tttcaaaaaa aggaaggata tcatcaaata
     4321 ccataacctt taattggggt agtactttga agatgtcgtc tggctcagct atatctattt
     4381 ctgtgatttc atctaatcgc cgacttgaag tataaatagt atcattctgc aattctatac
     4441 catatttatc ataatatttt ttcaagatat tttctacttc cggaaatgat ttaaaacctc
     4501 cgattacacc aggcaacata tatacaccag gatattttct ataactaatt gaaattcttt
     4561 cattattcct ttctaacgaa acattacctg caccaactcc tataggttta aaatctgata
     4621 attctaggta atctctaatt gaccttatta attgttgttt atccatttta tagtttattt
     4681 attcctgata ttgcttctcc attttcagaa tcaaatattt taatccattt attatttata
     4741 tttatttctt cattctttga aagcggagca ccatttacag aactgtcggg gaacttatct
     4801 ttactgcgaa ctgtcaataa ccctgccttt ttacctttat tttggtgggt ggtgagcctt
     4861 gtaccttcgc tgagtttgtc ttcaggtcag cctatggtta ttaggcattt aatcggactc
     4921 catctatcac attggaatat tccatatttt cgatagggtt aaagatgcac gtaaagtctc
     4981 cattcggatg attgggcagt gtttgtaaca ctaagtttcc gtttcccagc aatagccaat
     5041 cggatatttt tctcttttct cctttattgt caactaaatc aaattcccaa agaatagccc
     5101 catcagcctt atttatcttt ccaaacgatc catcatgtga agtgtaatat atgccaactc
     5161 catctacaaa gtgtaagtca ggtattctct caataggtgg tttgacttca atttctgtaa
     5221 gaacattgcc attataaata tccaactcta caactgataa tttttctatt ttcccatttg
     5281 gcaaaaagct ttttatatta ccaatcaata cagcctcgtt ctcatagtca atatggtgat
     5341 aactatttga gtatcgccat atttcttctc ctgtatctcc atttaaaaca atagtgtatc
     5401 tatccggatt gtaccagtct ctctctccag catgtccttt atctgttctg attttgtcaa
     5461 aggcatgata ctctaaaata atcaaattgt caaatgtttc aagtctaaca aattgccatg
     5521 aaaactccac cttccatatc tcatcccctc cgtcagggtt caaacatttt atccagttat
     5581 tctccttttc attattctga taaattaaaa ttccggaact aataaacttt gcgattatag
     5641 gatgatgata tttaaattct aaaacctttt cacctttact attaaaaact tcatattgct
     5701 tggttaataa gttatggtaa cacaatatat cttcccattt atactttgga aaatgatgac
     5761 caggaaaagt ggatatttga aatgtattga tatccaattt cttaattata ttatctcttg
     5821 tatgataaaa gtatttacat tcttcaaaat ttgaactgac attataccat aaatcgatgt
     5881 gttttagctc atgataatca ttttttgata aacattttat ccatccttga tgacttcccc
     5941 caactcctac ataatacacc tggttattta atataccggt acggttatac ggcattgaat
     6001 taaatattaa agtagaataa ttcattatgg atttataatt ttagaaaaaa ttgaagaact
     6061 agttatttgg tcaatcagag catccacatt tcctattgga atatcatcaa cattgctttc
     6121 aaccatagaa acatatttag cccacacaac ctcatcagtt gtattaaata aatcagattt
     6181 agactttaat gcctgattca gatcttccgg tgtaggtatc catctgtcag gatcaaattg
     6241 aaactcaaat ttggataagc tgttttgagt attgaagtag ttaatcaatt ggttaggaac
     6301 attgctcata ccgggtgctt tgcctcccca gcttttacat tcaataaact ttccattaac
     6361 gataatatca gcagaaaaat cacccgtagc ttcgtctata aaatcttcaa acttagtagg
     6421 agataaattc ttctttttaa caaacttcat cacccaatga gacccaattg cctgattctt
     6481 tccttgttgt aaaagttgat tcaatacagt ttgatagcct tcaatttctt taaactgctt
     6541 aaaatgatct aattggtcaa tcaattcatt tatccaaggc tttcctccat ttttgcacga
     6601 tgggcaacca aaaccatctt taatccactt attactaatt acctgatcaa ggttcaaagt
     6661 cttcaaataa tcatctgcga gagcttttga aagactttct aatacatcta aatcaactcg
     6721 taattgaata tgcccagcga ttttctccca cgccttcacc ctctccacct tatccaccaa
     6781 attagtcccg aaggccacat cctccaggtc ggcagctaac tttttagcat tctcaggagc
     6841 aatattaaca aataatgctt ctatgtggtg cttttttccg ggatattttg agagtatctg
     6901 gtcaaaactt tcctgagtca ggttaaggag gccggaggcg gtgttctggg ggtgaaatta
     6961 atcactacga agataactta ccaaatgagg ttaacattga tgatactccc tatcaatgaa
     7021 aaactacgac ttattgatca acttgatgat gaagataaaa aagccatctt ccgcattatt
     7081 gacggtttgc tcaccaaatc taaattcaaa gacttctttc agaaaaatgt ggcggctctt
     7141 taaagacaaa agcatcgtca aggaacttat cgatgctttt atcactaaaa agaaaattca
     7201 acaattagcg gggtgatcct ttcaaggttg atctataata atcaactaaa tccttaggat
     7261 agtattggtt atctctaaag ctactgtcat ctaaatctaa tattgctact atcgccgctg
     7321 attcaaatga ccaagcacca tgataccaac ttttatttgg ttctattaac atttgcgctt
     7381 ttttctgttc tttaatccaa tccctctcca aaaagatttt caccaacttt tcagcttcct
     7441 ccttttccga ttgaagtatt gcttttttca aattggagta aagcttaaag gtataatcat
     7501 catcttcctg tctcattttc caagagttta actttgatgc aaggatacac tcaaacaaaa
     7561 tatctataac attgtcatca ttgataattt ttcccaaacg aataaaaaaa tctttagaaa
     7621 ttacgcataa aaatcctatc gaaagaattg ttagaagttg tacatgagca tctatgttat
     7681 attggttcaa aactttatgt ttaggaccaa ttaattttct tgagccatga acccatgatt
     7741 cttctataag attaagaatt ggattaaaat catcaactaa ttcatcaatt ggtatcccta
     7801 gagaatattt agatactaat atatgaatta ggttggcagc catgttttgc ttaactggaa
     7861 taatcctatc aggttttatt tcaccctgat tcagcttcct agctctttct tcatatgatt
     7921 cagttcttga ctttatataa gatagaaagt gttctttatt tctatgtatg tctcgtacca
     7981 ttagattaag ttaaggtaac caatcaccaa gtggtccacc accttgattt aagtatccag
     8041 tttcactaac atatttataa gtaacacttc catcaggact aactttcgcc aatattctag
     8101 aataaccagc atctaaaatc tcatcaacta aattctgatc accgccaact gcctctaata
     8161 atctttgacc gttattctgt gtaatccatt catcgctcat ttgtctaggt aagccggttg
     8221 tcgggtctgc aggattcaac gaagcagaac ccttatattt cccctctaca ataaaatact
     8281 gaccgtcttt cttgacaaca atatctatcc cattatgtcc aggagaatcg atattgtcaa
     8341 ttcgtggttg aagggaagta tagccctttg cattcaaatc taaatcagct cctatttcac
     8401 caaaatttcc cttttgtttg ttcgtggcat ttgcaatatt ctcagcccat tcatcaagaa
     8461 ttgtccgctg attaagattg gaaatatttc ttctaaatgc aggtggcagt ccatcgtcta
     8521 caaacttctc ccacgccttc accatccccg gattcccatc aagcattctc agcatatcat
     8581 ccgatgcatc aaaaaagtct aataaaaact ggcttttccc ggcttcatct aaggcatcaa
     8641 ttctggtcgc aaggctgttg tatttattgc cgaggttttt taaaaaacac tcgccgttat
     8701 tgtgtaccaa aactcccgct ccgcccacgt aataattgtg attgtctcca attgtcagat
     8761 catatacttg tagggtggtg tcgatgagcg tcttgccgat gactctctca taatccccgg
     8821 aataactcca caaggtgtct ccgacagtca gagctgctac ttccttatat cctgaggtgg
     8881 tgtatatcgg atgactgggt gtcgcccaca gggtgtcacc ggagctcatg actaatgaaa
     8941 atagcgcagc aacaatataa ttttttattc ctgctattgg ctttaattcc tttattttct
     9001 ttgcaaaatt atacgaccac actttgtctc ccttattgat ggtttgtatc tctttccttc
     9061 ctgtttccgt ccaaacctcg gtcgagccgg aaaagcatcc aaaggtactt aacttacatt
     9121 caaaatcaca tatttgtccg cctattttta aatcaggtga taaggagagg atctcgtctg
     9181 taaattcttt ttgtattcct ctattttcca aacaaacatc tgcaaaatat ttatgtatct
     9241 cgtcaaattt ttgtagattt ttagcttcaa acttgccttt atataaggct ctggttttgc
     9301 tcatatcatt caccactttg ccaatttcta tcaccgagaa agctaaacct actccggcca
     9361 ttgctgcact tgttatctga atcctgtttc gcaatactgg atctatgaca tcttcatcaa
     9421 ttagttgaat tgccaaattg gacgaggaac caattacatc agccgccacc gcaatttttc
     9481 ttattacact tcctacttta aacgcagctt tgatctctgt aattccaata aaaagcccta
     9541 atacatccaa agcaatgtaa cc
//
LOCUS       DAOQQN010000071         9477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2695_length_9477_cov_5.874655, whole genome shotgun sequence.
ACCESSION   DAOQQN010000071 DAOQQN010000000
VERSION     DAOQQN010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9477
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2695_length_9477_cov_5.874655"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="PLG24_04100"
     CDS             complement(<1..876)
                     /locus_tag="PLG24_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24100.1"
                     /translation="MTQSKINIDNYEAYLLERFEGTIRAEDDLDLTVFLLENNLSDVS
                     SQDLFYFDDATILSEKETNLIDFNYLKQSTPIEQYDTLLFETVEGTITTEDWSELKHT
                     IEKNQILKRNYEAMMATKLAPDYSIIFKDKSSLKKVVVFRKMLYVLAAASFFGLFITL
                     GMRMYTNSTETHEHLVDNEIKVNDNQHSVSPGLVATDQESYSDSITPIGKKQEKMVDE
                     FAYPDNKTSVGIDHKPYYASGNPDDTQENQSESKEQDSSTPFKDISSKVESLQRIASV
                     RSAGVVSEQINYINII"
     gene            complement(878..1366)
                     /locus_tag="PLG24_04105"
     CDS             complement(878..1366)
                     /locus_tag="PLG24_04105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMZ24101.1"
                     /translation="MTVSEYNKSVDSYADNLYRFFLKNISDTELARDLVQETFAKMWL
                     KVGDVDYEKSKSFLFTVGYNTMIDHIRKKKRRDEFIAMQKPSEYEEKDYDLKEAIDFA
                     LSKLPVIQKTVLLLRDYEGYSYEEIGQMTSLSDAQVKVYLFRARKSMKEKLLENKLLF
                     PI"
     gene            complement(1708..2400)
                     /locus_tag="PLG24_04110"
     CDS             complement(1708..2400)
                     /locus_tag="PLG24_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ24102.1"
                     /translation="MNKTKIFYVEDDETLAFLTNEALVNEGFQVDHFNNGLDALNAFR
                     KNHYDICLLDVMLPKMDGFELAKKIRENNNSIPIIFLTAKSMLEDKIEGLTIGGDDYI
                     VKPFDMRELLLKVEIFLNRRSKSPAPGDIQITTFGDYTFDYANLILTYGQTTHTLTQK
                     EGDLLRYFSNHRNTLIKRSEILEELWGRNDYFLGRSLDVFISRLRKYLSSDSKLKIEN
                     VHGVGFRFSVDA"
     gene            complement(2397..3668)
                     /locus_tag="PLG24_04115"
     CDS             complement(2397..3668)
                     /locus_tag="PLG24_04115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMZ24103.1"
                     /translation="MTRSKVRLLTVIGTLTISAIIVIQGYWAIQMWNYKENEFSQTVK
                     VALYRVAKQLVELNGSVLPNTSIVNQMSSNYFVVNVNDKIKSDHVEYYLRKEFQKVLI
                     NEDFEFGIYDCSNDQVVYGNYIKANSEPINDAKEKSFLPTHEGFTYYFGVLFPNRSTS
                     LMANLKYVFILSILLLIALVVFVYSIFVIVKQNELSELQKDFINNMTHEFKTPLASIL
                     LANNAIAGNKKFAGDNTISRLTGIIAQQSKRLSSHVEKILDLAKLEKNKFLLFPEKID
                     LKQCISDMLPALELRINNLKGEFNVEMPEETVWIMADKVHLTNIIDNLLDNSIKYTVK
                     IPVINLSVVLIKNKVIITIKDNGVGMDQITLKKVYQKFYRSHTGNLHDVKGFGLGLFY
                     VKNICNALDWKLKIESQPEQGTTTTITIPRI"
     gene            complement(4222..5283)
                     /gene="porQ"
                     /locus_tag="PLG24_04120"
     CDS             complement(4222..5283)
                     /gene="porQ"
                     /locus_tag="PLG24_04120"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HMZ24104.1"
                     /translation="MSIKYIFAFTFCLLTDVLFAQIESNKIFPFLNQSSSGRMNALGG
                     YTLAEISPDINQSFSNPAFYNPDDQFDLSYNHQFLFKGAGVGNLALGKKIKWKDLALW
                     GGIQYAGYGSIDGYDENGIPTGLVKANDYNIIIGASTQLYENLRLGSNIKFITSNLGE
                     YSSNAFAVDLAAAYHVPDGTSTLGFAIKNLGRQITYYESTLEPLPLDIQASGSIRLKH
                     LPLRIGILAHHLNKWNLLYENPYDIEESFSFDDPFAKPDIKQYGLVDNLFRHLVIQAE
                     LAIGKNEGFKMRLAYNHMRKRDLTVYGFRSLAGFSGGLGLRIYKFGFDYGMSVYHLAG
                     ISHCLTITTDLNTFNRKSL"
     gene            complement(5365..5961)
                     /locus_tag="PLG24_04125"
     CDS             complement(5365..5961)
                     /locus_tag="PLG24_04125"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HMZ24105.1"
                     /translation="MRLTLFFPFLVMIFILSCSPDTKIIVQKDGENKILEKITVDART
                     QKKQGLYQRFYPSGNLAEEAYYDRDSLNGIRKFYYESGVLEQEQNYLSGIFQGPFRSY
                     YINGNINVEGNYVNNVMDGVWKRYYDTGELREEVNFVNNDENGPFREYYKNGNIKAEG
                     TYINGPNEQGELKEYNEDGELIKIMSCTNGICNTIWVK"
     gene            complement(6105..8216)
                     /locus_tag="PLG24_04130"
     CDS             complement(6105..8216)
                     /locus_tag="PLG24_04130"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMZ24106.1"
                     /translation="MGIITGIRKRGGLLIVAIGLAVFGFVVMDVVQNRNMAGSATRIG
                     TVDGSSMEFNEMRNMEEILYQGSTADEYSKREYIWNYFMESTILNKEAEKSGLMVGKE
                     ELMDLQFGNNLSPIMRQRYGNPQTGQVDIQQLQAIKDQIKKGELQPNMAAFWAIQEKE
                     IIKERLKDKLNSLFTKAVYTPNWLAEKIMADQAEPVTALYVKVPATYIGDTEVKLTDS
                     DYQKYIDEHKSVYTNEKESRIISTVNISVLTSSTDTMNAKQAIMDKMVEFKAATNDSA
                     FVLANNGTITNVYTKPDQLEEPLKSKISGLAVGDVFGPYIVGEEVKAAKLLGKTSVAD
                     SVKSRHILISAQTPEDFTKAEQRIDSAQAVILSKKASFADVAKAISMDPGSASKGGDL
                     PYAPQGMMVKEFNDALFFNSTPGQLKKVKTQFGYHLIEILDKKTIGDAFGYKLAYIDT
                     ELVPSEETQNKYLAEAEKLINGVTDVAQLTEKLKTTPGLQLQKNFAVSKPDFTLQTVG
                     PGQSARDIIKWAFESKRKVGDIATEPFPVQSTGKYYVEQYVIPVLTTIIPKGTASVAA
                     LKEELTPMVMAAKKIEMLVEKAKGVKSLEEMASKFSANIDTVQNVTFNQMQLPGIGRE
                     PKILAAIYTTPVDQTSAVIGGANGVFMIKPIRKEKSPVQSTIENTKMFYTASTMQMYS
                     GRLFDALRKKAKIEDNRGDIY"
     gene            8589..>9477
                     /locus_tag="PLG24_04135"
     CDS             8589..>9477
                     /locus_tag="PLG24_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HMZ24107.1"
                     /translation="MPKNPDIKSVLIIGSGPIIIGQACEFDYSGTQASRSLREEGIEV
                     TLINSNPATIMTDPVTADHIYLLPLTVDSIIQILEERKIDAVLPTMGGQTALNLCIEA
                     GEQGIWEKYGVKLIGVDLSAIELTEDRELFRKLMIDIGMQVAPSYIANSYLEGMEAAQ
                     KIGFPLVIRPSFTLGGTGGGLVMEEADFSEALKNGLNASPTHEVLVEKAVLGWKEFEL
                     ELLRDANDNVIIVCTVENLDPMGIHTGDSITVAPAMTLSDTAFQQMRNDAIKMMRSLG
                     NFCGGCNVQFAQNPETEELI"
BASE COUNT         2908 a         1796 c         1614 g         3159 t
ORIGIN      
        1 aataatgttg atataattaa tctgctcaga aaccacaccg gctgatctga cggatgcaat
       61 ccgttgtaat gattcaactt ttgacgaaat gtctttaaat ggtgtggatg aatcctgttc
      121 cttactttca gattgatttt cctgagtatc atccggattg ccagaagcat aatatggttt
      181 atgatcgatg cctactgatg ttttgttatc aggataggca aattcatcga ccattttctc
      241 ttgttttttt ccaatcgggg tgattgaatc ggaataactt tcctgatctg ttgctaccaa
      301 accaggagag actgaatgct gattgtcgtt aacctttatc tcattatcaa ccaggtgctc
      361 atgagtctct gttgaattag tatacatcct catacccaaa gtaataaaga ggccaaagaa
      421 actcgcagcc gcaagtacat agagcatctt tctaaagaca acgacctttt taagagagga
      481 tttgtctttg aatattatac tataatccgg tgctaacttt gtagccatca tagcttcata
      541 attccgcttc aatatctggt tcttttcaat ggtatgtttc aattcagacc aatcttcagt
      601 tgtaatggta ccttctactg tttcaaacaa tagcgtatca tattgttcta taggagtcga
      661 ttgcttcagg tagttgaagt caatcagatt tgtttccttt tcactcagta tagtggcgtc
      721 atcaaaataa aataaatctt gtgacgaaac atctgaaaga ttgttttcca acaaaaaaac
      781 ggttaaatca agatcatctt ctgcacgaat cgtgccctca aaccgttcca gcaaataagc
      841 ttcgtaattg tcaatattta ttttggactg tgtcatatta aataggaaat aataatttgt
      901 tttccaataa tttttccttc atggattttc ttgctctgaa gaggtaaacc ttcacctgtg
      961 catctgaaag tgaagtcatc tggcctattt cttcatatga atatccttca taatccctta
     1021 acagcaatac cgttttttgt ataaccggca acttactcag tgcaaaatca atggcttctt
     1081 ttaggtcgta atccttctct tcatattcac tgggtttttg cattgcaata aattcgtctc
     1141 ttcgtttttt ctttctgatg tggtcaatca tagtgttata tccaaccgta aaaagaaaac
     1201 tttttgattt ttcgtaatca acatcaccaa cctttagcca catttttgca aaagtttcct
     1261 gtacaagatc acgcgctaat tctgtatcag aaatattctt taagaaaaag cggtaaaggt
     1321 tatctgcata cgaatcaacc gatttattgt attcactgac tgtcacagtt ttgttttttt
     1381 gaatctgata atttcaggca tttcttttca ccaattgttt tgtttccaat aaaaatgaat
     1441 cattgaattc cctttctttt atagtgatac gatgggaatg gccaaatagt tacatcttgg
     1501 tatgtttttt caaaaaaaag atattaccga tgacacagta atatcttttg aatgagaata
     1561 ggtggtatag aatattaccg ccattcatta tgatttgatt gaattatagt tacttacgat
     1621 ttaagattta ctacaatgta tatactttag ttattcattt taagataaaa agtaaacggt
     1681 aaaatttttc ctggtcaaag ataagtacta ggcgtctacc gaaaatctga agccaacccc
     1741 gtgtacgttt tctattttca attttgaatc tgaagataga tattttcgaa gtctggaaat
     1801 aaaaacgtcc aggctacgac ccagaaaata gtcatttcga ccccacaatt cttcaaggat
     1861 ttcacttctc ttgattaatg tatttctatg attactgaaa tagcgtagca aatcaccttc
     1921 cttttgagta agggtgtgtg tagtttgtcc gtaggtaagg atgagattgg cgtaatcaaa
     1981 ggtataatct ccgaaggttg taatctgtat atctccagga gcaggtgact ttgaccttct
     2041 attgaggaaa atttcaactt ttaataataa ctctctcata tcaaatggct taacaatgta
     2101 gtcatcacca ccgatggtta ggccttcaat tttgtcttcc aacattgatt tggctgtaag
     2161 gaatataatc ggaatggaat tattattttc ccgtattttt ttggctagtt caaaaccatc
     2221 cattttgggt aacataacat ccaaaaggca tatatcataa tggttttttc gaaaggcatt
     2281 caatgcatcc aggccattat tgaaatgatc gacctgaaat ccttcattca ccagagcttc
     2341 atttgtcagg aaggctaaag tttcgtcatc ctcaacataa aagatttttg tcttattcat
     2401 attctaggta ttgtgattgt ggttgttgtt ccttgttcgg gttgactttc aattttcaac
     2461 ttccaatcca aagcattaca aatattcttg acataaaaca gtccaaggcc aaatccttta
     2521 acatcgtgaa ggttaccggt atggctccta tagaattttt gatatacttt cttcaaagta
     2581 atctgatcca tacctactcc attgtcttta atcgtaataa tcactttgtt tttaattaat
     2641 actacggaca agttaataac gggtatttta acagtatatt ttatcgaatt atccaataaa
     2701 ttatcaatga tattggtaag atgcactttg tcagccataa tccatacagt ttcttcaggc
     2761 atttcaacat tgaactcacc ttttagatta tttattctta actcaagggc aggcaacata
     2821 tccgaaatac attgctttaa gtctattttc tcagggaaaa gaaggaattt attcttctcc
     2881 aactttgcca aatcaagaat tttttcaaca tgagaactca atcttttact ctgctgagca
     2941 ataatgcctg taagtctgga aatggtatta tctccggcaa attttttatt accggctatg
     3001 gcgttatttg ccaggagaat actcgcaagt ggcgtcttaa actcatgagt catattatta
     3061 ataaaatctt tttgcagttc agaaagttca ttttgtttga ctataacaaa gatggaatat
     3121 acaaaaacta caagagcaat caataaaagt atactaagaa taaaaacata tttaagatta
     3181 gccatgagag aggtacttct attcgggaaa agtactccga aataataagt aaaaccttca
     3241 tgtgtcggta aaaatgattt ttccttggca tcattaatag gctcggaatt ggctttaata
     3301 tagttgccat aaactacctg atcattagaa caatcataga tgccaaattc aaaatcttca
     3361 ttgataagta ctttctgaaa ttctttgcgc aaataatatt caacatgatc agactttatt
     3421 ttgtcgttga catttacaac aaaataatta gaagacatct gattgacaat agaagtattt
     3481 ggcaaaacgg aaccgttaag ttcaaccaat tgtttagcta cccgatatag agcgaccttt
     3541 acggtctgag aaaattcatt ctccttataa ttccacatct gaatagccca atacccctga
     3601 atcactatga ttgcagagat ggtcaacgta ccaataactg taagcaacct tacttttgat
     3661 ctggtcataa tattccttcc ggaatgcttg ttattctatt tacaaagatg gtaattacaa
     3721 ataaatatgc atcctaatgt aaagttaatc aatctttgcg actgaaattc caatgaatca
     3781 gggattgttt gtatctaaaa cattgaggct ttatatttcg ataaaatatt gatacatatt
     3841 taaattaata aatatatata atgaatagat attatgagtt atatattaat tatctttgtt
     3901 tgggtaaaaa tattctgatt ttctttaccc ggaatattca tgaatactct ctgagttatg
     3961 attcgtagag ggtattttac tcaggctatt ttttttagat ttttacctgc atcggtttaa
     4021 acgcctggag cccatcaaat tcaggattgt attccaatcc tatgattttt tgttcctctg
     4081 tttttaggct atgcttagcc tctatttgaa tttacggtat aatagataaa aataatgaaa
     4141 cagaatcatg gaaaaaaggt agataatgaa ttccgggata tatttttaaa ccgcacactg
     4201 tatgcgatat tgagtaaaat gttacaaact tttgcgatta aaagtattta aatcggttgt
     4261 gattgtaagg caatgactga ttcctgccaa gtgataaaca gacatcccat aatcaaatcc
     4321 aaacttatag attctgagac caaggccacc ggaaaatccg gcaaggcttc taaacccgta
     4381 aacagtaaga tcacgttttc gcatgtgatt gtaagcaagt ctcattttaa aaccttcatt
     4441 tttgccaatc gcgagttcgg cctgaatcac gaggtgtctg aacaaattgt ctaccaatcc
     4501 atattgtttg atatcaggct tggcaaaggg gtcgtcaaaa gaaaaggatt cttctatatc
     4561 atatggattt tcatacaaca ggttccattt gttaagatgg tgagccaaaa taccaattcg
     4621 caaaggcaaa tgcttcaacc tgatacttcc tgaagcttga atatcaagtg gtaaaggttc
     4681 taatgtgctt tcataataag taatctgccg cccgaggttt ttaatggcaa atcccagtgt
     4741 tgaggtgcca tcgggtacat gataagctgc tgccaaatca accgcaaagg cattgcttga
     4801 gtactcacca agattagagg tgataaattt aatattggaa ccaaggcgaa gattttcata
     4861 cagttgcgtc gaagcaccta taattatatt ataatcattg gctttaacta gtccggtagg
     4921 gatcccattt tcatcatatc catcaataga gccatatccg gcatattgaa ttccgcccca
     4981 cagtgccaga tctttccatt ttattttttt ccctaaagca agattaccaa ccccggcgcc
     5041 tttgaaaaga aattgatgat tgtaactcag atcaaattga tcatctggat tgtaaaaagc
     5101 aggattggaa aagctctggt tgatatcagg gctgatttca gctaatgtat agcctccaag
     5161 agcattcatg cgcccactgg agctttgatt gaggaatgga aaaattttgt tgctctcaat
     5221 ttgagcaaac aggacatctg ttagcaggca aaaggtgaaa gcaaaaatat atttaattga
     5281 cattcagaac tcccaagtat ataaatcaac aaattttatg gactaaatat aatacttcat
     5341 gtttattccg tgaataaaaa atacttattt gacccagatc gtattacaga ttccattggt
     5401 acatgacata atctttatca actccccatc ttcattgtat tctttcaatt ctccttgttc
     5461 attcggacca ttgatatatg ttccttcggc ttttatattg ccatttttat aatactccct
     5521 gaatggtccg ttctcatcat tattgacaaa attaacttct tcccttagct caccggtatc
     5581 ataatatctt ttccacacac catccatcac attattcaca taatttcctt ctacattaat
     5641 attgccattg atgtaataag aacgaaaagg tccttgaaat attccggata gataattttg
     5701 ttcttgttcc aagactccac tttcataata gaactttcta ataccattta atgaatcccg
     5761 gtcataatat gcttcctcag ccaaattgcc ggatggatag aatctttgat acaagccctg
     5821 ctttttttgg gtgcgtgcat caacggttat tttttcaagg atcttattct ccccatcctt
     5881 ttgcacaatg attttagtgt caggagaaca tgataaaatg aagatcataa ccaaaaaagg
     5941 gaaaaataac gtcaatctca taacgtgaaa tttttaggac aatttcaaaa aaaacacgaa
     6001 tctggattag ataagaacta aaaaaccggg taaaaaattg tttgcaaata aaaaaaggct
     6061 ttccggtagg tgaccaaaaa gccttttcta tactaaatta aatattaata aatatctcct
     6121 cggttgtcct caatcttagc cttctttctt aaggcatcaa acagtctgcc actatacatt
     6181 tgcattgtac ttgcagtata gaacatcttc gtgttttcaa tggtggattg caccggtgat
     6241 ttctcctttc tgatcggctt aatcataaac acgccattcg cacctccaat aacagctgat
     6301 gtttgatcca ccggagtggt ataaatagct gccaaaatct ttggttcacg accaatgcca
     6361 gggagttgca tctgattgaa agttacgttc tgaactgtat cgatattagc actaaactta
     6421 cttgccattt cttctaaaga cttgacgcct ttagcttttt caaccagcat ttcaatcttt
     6481 ttagcagcca taaccatagg cgtcaattct tccttcaatg cagcaacact ggctgttcct
     6541 tttggaataa tggtagtaag gaccgggatg acatattgtt caacatagta ttttccggta
     6601 gattgaacag ggaatggttc ggttgcaata tctccaactt tgcgttttga ctcaaaggcc
     6661 cactttataa tgtcacgagc actttgacca gggcctactg tctgtagggt aaaatcaggc
     6721 tttgaaacag cgaagttttt ctgaagctgt aatcctggag tagttttcaa tttttctgta
     6781 agttgagcaa catcggttac accattgatt agtttttcag cttcagccag gtatttattt
     6841 tgagtttctt cacttggcac caattctgta tcgatatagg ccaacttata tccaaaagca
     6901 tccccaatcg ttttcttatc caatatttca attaagtgat atccaaactg ggtttttact
     6961 tttttcaatt ggccgggtgt ggaattaaaa aataaagcgt cattgaattc ctttaccatc
     7021 ataccctgag gagcgtaagg caggtcgcca ccctttgaag cactaccagg gtccatacta
     7081 atggcctttg cgacatcggc aaaggaagct tttttcgata aaattactgc ttgagcacta
     7141 tctattctct gttcagcttt ggtaaagtct tcaggtgttt gagctgatat cagaatatgt
     7201 cgtgatttta ccgaatccgc gacgctagtc ttacctaaaa gttttgcagc tttaacttcc
     7261 tctccgacta tataaggacc aaacacatct ccaacagcca aacctgaaat ctttgatttc
     7321 aatggctctt ccaattgatc aggcttggta taaacgttcg tgatggtacc gttgttagcc
     7381 aatacgaagg cagagtcatt agtcgcagct ttaaattcaa ccattttatc catgattgcc
     7441 tgtttggcat tcattgtatc agtagaggat gtaagaactg agatattcac tgtactgatg
     7501 attcttgatt ctttttcatt ggtatacact gatttatgtt catcaatgta tttctgataa
     7561 tcactatctg tcaacttaac ttcagtatca ccaatatagg tagcaggaac tttaacataa
     7621 agagcagtaa cgggctccgc ttgatctgcc attatctttt cagccagcca attaggtgta
     7681 tatactgctt tggtaaaaag tgaattcaat ttatctttca acctttcctt gatgatttct
     7741 ttttcctgaa ttgcccagaa agccgccata ttaggttgaa gttcaccttt ctttatctga
     7801 tctttaatcg cttgaagttg ctggatatcc acttgacccg tctgaggatt accatatcgt
     7861 tgtctcatga taggactaag attgttgcca aattgcagat ccattaactc ttctttacca
     7921 accatcaatc ccgatttttc tgcttcctta ttaaggatgg tactttccat aaagtagttc
     7981 cagatatatt ctcttttaga atattcgtca gcagtggaac cttgataaag gatctcctcc
     8041 atgttgcgca tttcattaaa ttccattgaa cttccatcaa cggtaccgat tctggttgca
     8101 gatccggcca tattcctgtt ctgaacgaca tccataacta caaaaccaaa tacagcaaga
     8161 ccgatagcga caatcagtaa tccacctcgt tttctaatac ctgtgattat tcccatgtaa
     8221 tatgcgttgt tttttctatg ttttacaaaa atccgtgcaa aggtaatatt taattgtaat
     8281 ttaaagactt tttttgtaaa tattataaaa atttatatat tgcctgaata tcataccact
     8341 aaatattaaa aaatttaagc attgtcacaa ccatttcttt tatttaagat acattcctat
     8401 aaatattttt cgaacatttc ttacctttgc ggcgccttta aagaaacagt ccggaaagtt
     8461 ggaatgtaaa gtttgatttg ccttggatat tataatttca aagcatagga agactaaaac
     8521 tttattttga aattaagtat tgagatgttt cttcaaggcc tggactaata attaacaaac
     8581 tacatagaat gccaaaaaat ccggatataa aatccgtcct cattatagga agcggtccca
     8641 tcatcattgg tcaagcctgt gaatttgatt attcaggtac acaagcctca cgttccttaa
     8701 gggaggaagg tatagaggtg actttaatca actccaatcc ggcaaccata atgacggacc
     8761 cggtaaccgc tgaccatata tacttattgc ctctcacagt agattctatc attcaaatcc
     8821 tggaagaaag aaaaattgat gcagtccttc ctaccatggg aggtcagacc gcactaaatc
     8881 tatgcattga agcgggagaa caaggtattt gggaaaaata cggagtaaaa ttgattggtg
     8941 ttgatttgtc cgctattgaa ctcactgagg accgcgaatt attccgcaaa ctcatgattg
     9001 acataggtat gcaggtggca ccatcctata ttgccaactc atatctggaa ggtatggaag
     9061 cggcgcagaa aattggcttc ccactggtaa tcagaccatc ttttaccctt ggcggtacag
     9121 gtggaggatt ggttatggaa gaagcagact tttcggaagc tttaaaaaat ggacttaacg
     9181 cctcccctac tcatgaagtg cttgtagaaa aagctgttct gggctggaaa gaatttgaat
     9241 tggaactatt acgggatgcc aatgacaacg ttattatagt atgtactgtt gaaaatctgg
     9301 atcctatggg tatccacacg ggagatagta ttactgtggc tcccgccatg acccttagtg
     9361 atacagcttt tcaacaaatg cggaatgatg cgatcaaaat gatgcggtca cttggtaatt
     9421 tttgtggggg atgtaatgta cagtttgcac agaatcccga aacagaagaa ttgatag
//
LOCUS       DAOQQN010000072         9412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2724_length_9412_cov_4.848028, whole genome shotgun sequence.
ACCESSION   DAOQQN010000072 DAOQQN010000000
VERSION     DAOQQN010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2724_length_9412_cov_4.848028"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PLG24_04140"
     CDS             complement(<1..573)
                     /locus_tag="PLG24_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24108.1"
                     /translation="MSTRLTPFSRLLIVVAILSGVVFGGKYLIDKGVFGGSSSTDNSN
                     TDSSIKQGKTLPIDDDVLNVQVFTFGNAAPGLYFNNGTEPNENSRFYKDFGLKVKFHV
                     IDDFDASRQAFKGDKVQLFNNETSAMATEMEGLAPFDPQVVMQLDWSRGADAVVARRG
                     INSINDLKGKKVAVTPSTPSQTLLLFMLEAA"
     gene            complement(610..1635)
                     /locus_tag="PLG24_04145"
     CDS             complement(610..1635)
                     /locus_tag="PLG24_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24109.1"
                     /translation="MDQQSFTPKSFWQRPEGKTGVVFGIGILAGLGYLLYKTLPFLIS
                     LTSNLLYLSFLLIALGAILYMVLDPKMRNLVWYMYKSIMRWITGIFVQIDPIGILKSY
                     VDTLKNNLSKMDQQISKLRGQMHKLKEVIMNNKKQITANLNLASEAKERNKESMMILK
                     SRQAGRLTDSNMKLEDLYKKMEILYRVLTKMYENSEIMMEDIKDQVSVKEQERNAIRA
                     SHSAMKSAMSIISGDKDQRAMFDQALEAIADDVSAKMGEMERFMEMSSNFMDSVDLQN
                     GVFEEEGLKMLEMWEKEGASLLLGTEKTKIINKANDDDDVLDLNAPIPHPVKEEARLN
                     QYDSFFE"
     gene            complement(1646..2317)
                     /locus_tag="PLG24_04150"
     CDS             complement(1646..2317)
                     /locus_tag="PLG24_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24110.1"
                     /translation="MKSIFVEAVDDGSQPVNAETNSEVKAETNNTDTSKAYSENLTNH
                     TSSNTEGQQSDQFYKVLFGALEANNQPGFDYLEFKKALMGLDNLGMDEKTKYLSAFAG
                     AKASGATAQGLMDSAVKYLDVLLDESNKFKNAVEIQKSKQISNRENELGQLNNLISGK
                     TEQINKLQAEIEEHKNMLKSLESEISNAREKIEITIADFNVTYDGLVMKIKNDVDRIK
                     TYINN"
     gene            2860..3201
                     /locus_tag="PLG24_04155"
     CDS             2860..3201
                     /locus_tag="PLG24_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HMZ24111.1"
                     /translation="MTENSLLQSEKLYFSISEIAESFGVSASLIRYWETEFTFLKPHK
                     NSKGDRRFTKQNIEQIQLIYHLVKERGFTLEGAKNEINEIRKRQMTIKSLSNKLKSIK
                     QGLEGLKKNLE"
     gene            complement(3229..4014)
                     /locus_tag="PLG24_04160"
     CDS             complement(3229..4014)
                     /locus_tag="PLG24_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ24112.1"
                     /translation="MKRKEGIVTLTGTGTSQGVPVISCTCAVCRSDDPRDRRFRTSAH
                     VSFETGAFMVDIGPDFRLQCLGNNIDRLDGIFVTHEHIDHVNGLDDIRPFNYIQKKVM
                     PIYGEERVLNEIRKRNSYIFEPSTYAGLPMLELRNIEAFSPVDFQGMLVMPMRIMHDR
                     LPILGYQFGNFAYVTDAKTFVPETVSILREMDTVVINALHHRKHHSHLNLEEALDFIS
                     ENRLKKVYLTHISHQMGQYKEINQKLPGGVELAYDGLKINFRY"
     gene            complement(4017..4256)
                     /locus_tag="PLG24_04165"
     CDS             complement(4017..4256)
                     /locus_tag="PLG24_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24113.1"
                     /translation="MSNKEKANIKTLVGEIHSLGYRVIFEKGNFQSGHCIVLDRKVIV
                     INKFLSDKVKVLTLKDLLYQIRKEESEHGKFSVPN"
     gene            4408..5019
                     /locus_tag="PLG24_04170"
     CDS             4408..5019
                     /locus_tag="PLG24_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HMZ24114.1"
                     /translation="MRLLILCLLITGIIYTGIGQSNFTGGIIAGGSLSQVTGDEAFGY
                     NKASYLVGVKAGYHFKTRLNIEVEFLYNIKGSARGRRFNYLPYWTLEFKYLEIPLLIG
                     YKDWRNTKGFYHMVFYGGLSYAKMIDSKLTNPKYEPLMTGNLNKNEFSFILGATYNVN
                     KHLGFTFRYNRAITKVWENSIVDDRFSRWMKNYQISLAAQYMF"
     gene            5021..5911
                     /locus_tag="PLG24_04175"
     CDS             5021..5911
                     /locus_tag="PLG24_04175"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24115.1"
                     /translation="MAIEAVIDLGTNSFNLLIGRSEDGKKEIMYRESRIVQLAKEGIT
                     PIGDASLQRAMDALDVFQEKISNYKAERVRVIGTSALRSASNSDDVISKIRSYYHWEV
                     SIIDGEAEARYIFVGTMEALQISDETCLIMDIGGGSVEFILTKAGKMLWWTSVPIGVQ
                     YLYLHFGRNVPTMSEEVAALERLLDLELAGLKKAIRQFKPERLIGASGAFDELLQMSA
                     IPAGDKFTRLDTPIFYENFEKLQKQVTERRNVSTMRTEERPEIVMTGFLLIRYVLQLI
                     KVPEIIATPFALREGVFFMG"
     gene            complement(6365..6916)
                     /locus_tag="PLG24_04180"
     CDS             complement(6365..6916)
                     /locus_tag="PLG24_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HMZ24116.1"
                     /translation="MKFFNIILLFAAVSLVSCSSPRKSISTGRSPAKSHHKDLSGAER
                     EKASARTPEGYLRAEGLAADKRLIRLVDDWLGVPHRIGGKDKSGIDCSGFVTVAMNKV
                     YSKDFSGPSAVMATRGKAIRKNELKEGDLVFFKINGPKVSHVGLYLSDGYFVHATVKK
                     GVMISSLSEPYYEKFYSGSGRVL"
     regulatory      7140..7352
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            7475..8518
                     /locus_tag="PLG24_04185"
     CDS             7475..8518
                     /locus_tag="PLG24_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24117.1"
                     /translation="MKKLFFLFFASIMITACTKDDPGGDPGIYDNGVFVVNEGPFGTG
                     TGTLTFIGDDGNLTQKVFEKENGNVVLGNIAQSMIWHNNRYYIVVNNANKIVVADKDM
                     KVVGEITGLTGPRFMVAQGNKAYISEWGENYEKGGVAIADLTSLKVVKQIPTGKGPEA
                     LLLDGNQLLVPNGGSYNASTFETLPDSTISVIDIGSESVTKVISVKYNPNSIIKAGNG
                     YLVASAEKSFGTNNGSIYFIRSSDNSVTKVAGATTGSYAKLHKVDETKSVVISGFAEA
                     LLLTFDAAAVSMTQKALDFAYSVDYDIKRKLIYIADAGDFSSEGKVITYNVNGTKEKE
                     FAAGIIPGNFCFR"
     gene            complement(8623..>9412)
                     /locus_tag="PLG24_04190"
     CDS             complement(8623..>9412)
                     /locus_tag="PLG24_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HMZ24118.1"
                     /translation="ISGYAAQLDYGLKDIRHSIKHLSELALGGTAVGTGINTPPGYAV
                     NVAKHIADLTGIPFITAPNKFESLAAHDAIVSAHGALKTVAVSMMKIANDIRLLASGP
                     RCGIGEISIPDNEPGSSIMPGKVNPTQCEAMTMVAAQVMGNDVAINVGGSTGHFELNV
                     FKPVMIRNFIHSAKLIGDVCVSFDERCARGIEPILPNIKNNLDHSLMLVTALNTRIGY
                     YKAAEIAQTAHREHKTLKQVAVELGYVSPEDFDAWVRPEDMVGL"
BASE COUNT         2850 a         1963 c         1862 g         2737 t
ORIGIN      
        1 ggcagcttcc agcataaaca ataacagagt ctgagaaggt gtagatggag taaccgccac
       61 ctttttccct ttaaggtcat tgatactgtt gatacctctg cgggcgacaa cagcgtcagc
      121 acctctcgac cagtccagtt gcatcaccac ctgaggatca aatggtgcca aaccttccat
      181 ctcagttgcc atagcgctgg tctcattatt gaataattgt accttgtcac ccttgaatgc
      241 ctggcgggaa gcatcaaaat catcaatgac atgaaacttg accttcaggc caaaatcctt
      301 ataaaatctg gagttctcgt ttggctctgt accgttgttg aaatacaagc ctggtgcagc
      361 attgccaaag gtaaatacct gaacattcag gacatcatcg tcaattggca gggttttgcc
      421 ttgttttatt gacgagtctg tgttggaatt atccgtggaa ctactaccac cgaaaacgcc
      481 tttatcaatc aggtattttc caccgaatac cacacctgaa agtatggcca ctacaattaa
      541 gagtcgggag aagggagtta atcgtgttga cattttgttg ttttttaaaa ttataaaaat
      601 ctaagctctt tattcaaaga aggaatcata ttgattcaat cgtgcttctt cttttaccgg
      661 atgtgggatg ggagcgttca gatccagcac atcatcatcg tcattggcct tattaatgat
      721 cttggttttt tcggttccca acagcaatga tgcgccctct ttttcccaca tttccagcat
      781 tttgagtcct tcttcctcaa agacaccatt ttgcagatct acactgtcca tgaagttgga
      841 agacatctcc ataaatcgtt ccatttcacc cattttggca ctcacatcat cggctatagc
      901 ctccagggcc tgatcaaaca tggctctctg atccttatct cctgatatga tggacatagc
      961 ggatttcatg gcactatggc tggcacggat tgcgtttctt tcctgttctt tgactgagac
     1021 ctgatctttg atgtcctcca tcataatctc actgttttcg tacatcttgg taagaacacg
     1081 gtagaggatt tccatctttt tgtaaagatc ctccaatttc atattagaat ctgtcaacct
     1141 accggcttgc cgggatttta gaatcatcat ggactcctta ttccgttctt ttgcttcact
     1201 tgccagatta aggttagccg tgatctgctt cttattgttc atgatgactt ctttaagctt
     1261 gtgcatctga cctctaagct tggatatctg ttgatccatc ttcgaaaggt tattcttcag
     1321 cgtatccaca tatgatttta ggatgccgat tgggtcaatc tgaacaaaga tacctgtaat
     1381 ccaacgcatg atacttttgt acatatacca aaccaaattt ctcattttag gatcgagaac
     1441 catataaaga atggctccca aagcaatgag caggaatgac agatacaata aattggatgt
     1501 cagcgaaata agaaaaggta gtgttttata caaaagatac cccaaacctg ccagaatacc
     1561 tataccgaaa acaactccgg tcttgccttc aggcctttgc cagaatgatt taggcgtaaa
     1621 tgattgttga tccataaaat tgaaattaat tatttatata cgtttttatc ctatcgacat
     1681 cattcttgat cttcatgaca agaccatcat atgtcacatt aaaatctgca attgttattt
     1741 cgattttttc cctggcatta gaaatctcgg actcaagtga cttcaacatg tttttatgtt
     1801 cttcgatttc agcctgcaat ttgttgattt gttcggtttt cccggaaatc aagttattca
     1861 gttgccccaa ctcattttcc cggtttgata tttgctttga cttctggatt tctaccgcgt
     1921 ttttaaattt gtttgattca tcaagcaaaa catccagata ctttactgca ctatccatca
     1981 agccttgtgc agtcgcgcca ctggcttttg caccggcaaa ggcacttaga tatttggttt
     2041 tctcatccat acctaaatta tccaggccca tcaaagcctt tttgaactca agataatcaa
     2101 atcccggttg gttattagct tccaaagcac caaaaagaac tttgtaaaat tgatcagatt
     2161 gttgcccttc ggtgttgctg cttgtatgat ttgtcaagtt ttccgaatac gcttttgaag
     2221 tgtcagtgtt gttggtttca gctttcactt ctgaatttgt ctcagcattt accggttggc
     2281 tgccatcgtc aactgcttca acaaaaatag acttcatttt ctttaaaaaa tcactcattt
     2341 tgactcaaaa ctatgcaaga aaaatcaatg gaactgtatt tatatcgtcc tatacggatt
     2401 gctaaggtaa gaaaaaaagc catagtacaa aaaattcgat gtgacgattc aaagaaaaga
     2461 ctacttttta ccctaataag tatcaaaaac acgatatttt tcgaaacatt ccatgaaatt
     2521 atcgaaatat gttaatttta attaaatgcc gatagtttcc tggcggtcaa aaaaatcgac
     2581 aaaattcagt aaatagtccg accaatggtc tccctttttt actttgagat ctaattataa
     2641 agaaatattc aacccggctt ttcattcaaa tgttccaaga gtaatttctg acctgaacga
     2701 taactttcgt cgaaacgact gcaacaacaa tcatcataaa catttaaaaa aaaattatac
     2761 ataaaaattc aatatattaa accaataatt gtatctttgt aatgtaactt attgataata
     2821 agccatccca ccattattat taaaagttga cttacaacta tgaccgaaaa cagtctattg
     2881 caaagcgaaa aattatattt ttcaatcagt gagatagctg agtcatttgg cgtatctgct
     2941 tctctcattc gttattggga gacagaattc accttcctca aaccccataa aaacagcaag
     3001 ggtgacaggc gatttaccaa acagaatatc gagcaaatcc agcttatcta ccatcttgtg
     3061 aaggaaagag gctttacact ggagggagcc aaaaacgaaa taaacgaaat ccgaaaaagg
     3121 caaatgacca tcaagtcact ttccaacaaa ttaaaatcca tcaaacaagg gctggaaggt
     3181 ttgaaaaaga atcttgaata atcatccaat ttatactttc cctattgatt aatacctgaa
     3241 attgattttc aatccatcat aggctaattc gaccccgccc ggaagttttt gatttatttc
     3301 cttgtactga cccatttgat ggctaatatg agtgaggtaa acttttttga gccggttttc
     3361 ggaaataaaa tccaaggctt cctcaagatt cagatgtgaa tgatgcttac gatgatgcaa
     3421 tgcgttgatc actactgtat ccatctctct caaaatggag actgtttcag gaacaaaggt
     3481 tttggcatca gtcacatagg caaaattgcc aaattgatat cccaggattg gcagacggtc
     3541 gtgcatgata cgcatgggca taaccaacat accctgaaaa tcaaccggac taaatgcttc
     3601 aatatttctc aattcaagca ttggcagacc tgcataggtt gaaggttcga agatgtaact
     3661 gttgcgcttt ctgatctcat tcaggacacg ttcttcacca tagatgggca tgaccttctt
     3721 ctgaatataa ttgaagggtc ggatatcatc caaaccgttg acatgatcga tgtgttcatg
     3781 cgtgacaaaa atcccatcca gtcggtcaat attatttccc agacattgca gacgaaaatc
     3841 cggacctatg tcaaccatga aggcgccggt ttcaaagcta acatgagccg aggtcctgaa
     3901 acgccgatcc cgagggtcat ccgacctgca taccgcgcat gtacaggaaa tgacaggaac
     3961 cccctgagat gtccccgtac cggttaatgt aacaatgcct tctttccttt tcatcgtcaa
     4021 ttggggacgg aaaatttgcc gtgctcactt tcttctttcc tgatttgata taacagatct
     4081 ttcaatgtga ggaccttaac cttatcggat agaaatttat tgattacaat gactttccta
     4141 tccaaaacga tgcaatgccc ggattgaaaa tttccttttt caaaaatcac tctataaccc
     4201 aaagaatgaa tctcaccgac gagtgtcttg atattggcct tctccttatt tgacatagat
     4261 atgaagagta attaaccgct aagataatgt taatcgggaa aaaatgaaat tggctttaga
     4321 ggggagtgac gatattccat accacatcaa tcacaaattg tagacttgta cgttatttat
     4381 ttacatttgc agtattaaag aaaggatatg cgtcttttga ttttatgcct gttgatcacc
     4441 ggaattatat atacgggtat aggccagagt aactttaccg ggggaatcat cgccggagga
     4501 agtctctcac aagtgacagg tgacgaagca tttggatata acaaggcctc ctatcttgta
     4561 ggcgtgaagg caggttacca cttcaagacc cgactcaata ttgaagtgga gtttctctac
     4621 aacatcaaag gcagcgcacg tggacggcga ttcaactatc ttccctattg gacccttgaa
     4681 ttcaagtatc tcgagattcc tcttttgatt ggttataagg actggcgcaa cactaaaggt
     4741 ttctaccaca tggttttcta tggcgggttg tcctatgcca agatgattga cagtaaactg
     4801 accaatccaa aatacgagcc ccttatgaca ggcaacctca acaaaaacga attttccttt
     4861 atcctgggag cgacttacaa cgtcaataag cacctgggat tcacattcag gtacaacagg
     4921 gcaatcacta aggtttggga aaacagtatt gttgacgatc gattttcccg ctggatgaaa
     4981 aattatcaaa tcagtctggc tgcccagtat atgttctgat atggctatag aggcagtcat
     5041 agaccttggt acaaattcct tcaatttact aatcggcaga tcggaggatg gcaaaaaaga
     5101 gatcatgtac cgggaaagtc ggatcgtcca gctggcaaag gaaggcataa cgcccattgg
     5161 agacgcatcc cttcaaagag caatggatgc attggatgta tttcaagaaa aaataagcaa
     5221 ctacaaagct gagagagtgc gggtcatcgg cacttctgct ttgcggagcg cttcaaatag
     5281 tgatgatgta ataagtaaga tccggtcata ttatcattgg gaagtttcaa tcatcgatgg
     5341 tgaggcagaa gcaaggtata tatttgtcgg caccatggaa gctttgcaga tatctgacga
     5401 aacttgcctg atcatggaca tcgggggcgg aagtgtggaa ttcattctta ctaaagcggg
     5461 taagatgctg tggtggacga gtgtgcctat aggcgttcaa tatctttatc tgcactttgg
     5521 gcgtaatgtc cctaccatgt ctgaagaggt tgccgcccta gaaaggttat tggatctcga
     5581 actcgcagga ctaaagaaag ccatcagaca attcaaaccg gagcgactta tcggagcttc
     5641 cggtgcattt gatgaattac tccaaatgtc ggctattcct gccggagata aatttacccg
     5701 attggacact ccaatattct atgaaaactt tgaaaaactg caaaaacaag tcacggaacg
     5761 gaggaatgta tccacaatga gaacggaaga aaggcctgaa atcgtgatga caggattcct
     5821 attgatacgt tacgtattgc aattaattaa ggtcccggaa ataatcgcta cgccctttgc
     5881 attgagagag ggggtgtttt ttatgggata aaaaaaagtt gaagagtccg gggtcggata
     5941 cattgggtaa gagatttgag aattgaaata cttctcggca tgaaggtgca aacacctggc
     6001 ttacaataag ttattttaca gcttgaaaga aatcaaatgg aagaacagtc gttgggttga
     6061 caattgacat cgattagcaa acctgaattc actaaaagga atcaactttg gccaattgcc
     6121 ggacagttaa aacaacggta caatcatcag tacagcttaa aatatttttg tcacatatat
     6181 gaaagcgtcg atgtgttgat agaaagaatt aatagaataa aacattcaaa tcaggggatt
     6241 gtaaataaaa acaataaata tctaaatatc ttgttttcaa aaattaacat attaaaattt
     6301 acctatcaac atctgactgt ctaaataacg aaaactgaaa agattaagaa caaaaccgcc
     6361 ttaatcacag cacccttcct gaacctgaat aaaatttttc ataataaggc tcactcaatg
     6421 aagaaatcat aacgcctttt ttgacagttg catgtacgaa ataaccatca gaaagataga
     6481 gaccgacgtg ggaaacttta gggccattaa tcttaaaaaa gacaagatct ccctctttca
     6541 attcattctt cctgatcgcc ttccccctgg tagccatcac cgctgaaggc ccggaaaagt
     6601 ctttactata tactttgttc atggcaactg ttacgaagcc cgaacaatca attccactct
     6661 tatccttccc tcctattctg tgtggcacac ccaaccaatc atctaccaga cgaatgagcc
     6721 ttttatcagc agcaaggcct tctgctctga gataaccttc cggagttctg gccgatgcct
     6781 tttcccgctc tgcacccgat aaatccttgt gatgactttt agcgggagac ctgccggttg
     6841 aaatgctctt ccttggagaa ctacaggaaa ccaaggaaac agctgcaaat aacaatatga
     6901 tattaaagaa tttcatcact tatatattaa cgacaaaatt aatctatcat acgttattaa
     6961 tcaacatatt aaatgttaaa atattcatat atataaataa aaatgtatat ttagctgggt
     7021 aagtgaggct tttgtacaaa acagaggaaa gagaagccga tgattcattc agccgaaaaa
     7081 ttactgaagt tatttgacaa ttttaattta tggttaatcc aaatacatat attcatatta
     7141 tctttgtcgc gcaatttggt ttcctgagta taatatgctt tggaattaaa tgggaaccgg
     7201 gtgagaatcc cgggcagttc ccgctgctgt aacatctttt ccgtgtttac ggaataatgg
     7261 aaaggcctta catgccactg tttatggata aatgggaagg cgcctgaccg catgatgaag
     7321 ccagaagacc tgcctgttgc gatgtgttcg atctttcgga gaaaaggatt ggtacatggt
     7381 cagggcttat gcttctcgct ttgtatttta cttctattct tcagagataa aattgattta
     7441 ttaaccggct ataaaggccc aaaattttat tctgatgaaa aaactatttt ttttattctt
     7501 cgcttcaatt atgattactg cctgtactaa ggatgatccg gggggggatc cgggtatata
     7561 cgacaatggt gtttttgtag tcaatgaggg accctttggg accggaacgg gcacgctcac
     7621 attcattgga gatgatggca atctgaccca aaaggtcttc gaaaaggaaa atggaaatgt
     7681 ggtgctcgga aacatagctc agtcgatgat ctggcacaac aaccgatatt atatcgtggt
     7741 caacaatgcc aataagattg tagtagcaga taaggacatg aaagtggtcg gtgaaatcac
     7801 cggactgaca ggtccgcgtt tcatggtagc tcagggcaat aaggcatata tcagcgaatg
     7861 gggagaaaat tatgaaaaag gtggcgtggc tatagcagac ttgacttctc ttaaggttgt
     7921 gaaacaaata cctacaggaa aaggccccga agccttacta ttggatggca atcaactcct
     7981 ggtaccaaat ggcggttctt ataacgcctc caccttcgaa acactacccg actccaccat
     8041 atctgtcatc gacataggaa gtgaatcagt gaccaaagtc atttctgtca aatacaatcc
     8101 aaacagtatc atcaaagcgg gcaatggata tttggttgct tcggccgaaa agagtttcgg
     8161 taccaacaat ggcagcatat attttatccg tagttctgac aattcagtta cgaaggttgc
     8221 aggagcaaca acaggaagct atgcgaaact gcacaaggtc gatgaaacca agtctgttgt
     8281 tatctccggc tttgctgaag ctttgttact aacttttgat gctgcagctg taagcatgac
     8341 tcaaaaggcc cttgactttg cgtattcagt ggactacgat ataaagcgaa aattgatata
     8401 catcgctgat gcaggtgatt tctcaagtga gggaaaggtc ataacctata atgtaaatgg
     8461 aacaaaagaa aaagagtttg cagcaggaat tattccgggt aacttttgtt tcagataatc
     8521 acctggtcaa agccgaataa ttcacaggct aatcagtatc ctgattgcat aaaaaaaaaa
     8581 ggcggtcgca agggccgcct tttttttact ttagctgaca aatcacagcc ctaccatgtc
     8641 ttccggcctt acccatgcat caaaatcctc agggctaaca tagccaagtt ctacagccac
     8701 ctgcttaagt gtcttgtgtt ctctatgagc cgtctgagct atctcggctg ctttataata
     8761 gccgatcctt gtattaaggg ctgtaaccag cataagagaa tgatccaggt tgttctttat
     8821 gttgggaaga ataggttcta ttcccctggc acaacgttca tcaaaggata cacatacatc
     8881 gcctatcaat tttgcactgt gaataaagtt tctgatcatc accggtttga atacattcaa
     8941 ctcaaagtgt cctgtagatc caccgacatt gatggccacg tcgttaccca ttacctgagc
     9001 tgccaccatt gtcatagctt cacattgtgt aggattgacc ttaccgggca tgatggaact
     9061 tcccggctca ttgtcaggaa tagaaatttc tccaatgccg caacgtgggc ctgaagccag
     9121 cagacggata tcattggcga tcttcatcat actaactgca acagttttta gggcaccatg
     9181 tgctgacaca atggcatcgt gagctgcgag cgattcaaat ttgttgggtg ccgtaatgaa
     9241 agggattcct gtcagatcgg cgatatgctt tgctacattg actgcataac cgggaggcgt
     9301 attgatgccg gtgcctactg cagtacctcc aagggccagt tcactgaggt gcttgatact
     9361 gtggcgtata tccttcaatc cataatcaag ttgtgcggca taaccggata tt
//
LOCUS       DAOQQN010000073         9374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2742_length_9374_cov_5.949887, whole genome shotgun sequence.
ACCESSION   DAOQQN010000073 DAOQQN010000000
VERSION     DAOQQN010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9374
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2742_length_9374_cov_5.949887"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PLG24_04195"
     CDS             <1..901
                     /locus_tag="PLG24_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HMZ24119.1"
                     /translation="MQQQLRLEGEYPFLLATPLGVKGSFKLFRSDSSFIEKIIEFGGQ
                     YYFGYDSKLEAFWKRQQSDINTVDTSKVIATKKLPASLDYRTDYFGLTYNIQHLDFRL
                     NPRKGFSVDVQMGIGQRRIFKNDKILAIKGPDQSGFDYRTLYDTVTLRSTLFRPVIDS
                     RIFFPLFKRMAIMIRANGAMIAGPKLVYNNEKFRIGGNKTFRGFNEQSFYTTSYLIAT
                     TEVKMVLDELSALFVFADNGWINSTSTIRLNNYLWNMGVGAGINFGTKVGVFGLSIAV
                     GKNRSTPFNLQAAKIHFGYQSLF"
     gene            complement(968..2995)
                     /locus_tag="PLG24_04200"
     CDS             complement(968..2995)
                     /locus_tag="PLG24_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24120.1"
                     /translation="MKRIILFIAILGTAFQVVGQVNQDSLQEAFLDSIQFRELGEVVV
                     ISNATARSMKDSKALGSLDNYLKNSSSVNMVRRGAYAWEPLLNGMATERSVITVDGMR
                     IYHACTDKMDPVTSYVENTNLSKANINDGQSGAEHGGTIAGSIDLVRRKSGFKTFPKF
                     GGSAFAGFESNNMQQIYGATLNYGSPNFFTDVDFTYRDAENYKAGQRSGLSSEVLYSQ
                     FTKYNLSAISGFKFNDRQHVEASVIFDKATNVGYPGLPMDVALAQATIASLQHSYRSP
                     SPHVDFIETKAYYNTITHIMDDSRRPVVPIRMDMPGWSKTAGFYSKLAGNYNRHTFKA
                     TLSGHLNNSLAEMTMHPNNPNEADMYMLTWPDVNTIYAGINIEDNISLNDKLNLSIQG
                     GIGGHYNEIRSETGLNSLHLFYPDLKATKLRSIKNLSGHLQLKNGRFQHKMGIGYGDR
                     APSVSEAYGFYLYNVNDNYDYIGNPNMKNEKSFSLEVSSGYTAEKISVRWKANYFYIK
                     DYIIGKPQDEFFPMYITASGVKVYQQLEYAGILNTAMNVSYNILKDLLFQADASYRYG
                     QGAQETILPSIQPLGYSAKLKYQKNSFFAEAGIEGCSKNRNSPEFGEIQKPAYMVANV
                     ALSNDFSIGNINLVVKLGVENLFDKYYSTFDDWFGIPKMGRNIFTNVIFKF"
     gene            complement(3068..3940)
                     /locus_tag="PLG24_04205"
     CDS             complement(3068..3940)
                     /locus_tag="PLG24_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HMZ24121.1"
                     /translation="MKNIKIFMLFLIASLFLISCTKEEIKNPINNINETEGLLLVKSF
                     ESNDIKAEVFNGSGTLSVGFNKIFIRLTDKQGNFIKQSSISLLPMMTMNMHGNTHQHS
                     CPVSGITKVEGKETLYEGYIVFIMPSGDSENFWELTFTCNLDGQTYKISDKINVLPSE
                     TEFSKKFSSATGSDGKKYFLALVEPAQPKIGVNDIVVALFQRDDSGNFPIVDNYKMMV
                     DPRMPGMGNHSAPGNKDLEQLSDGLYHGKVGFSMSGYWKINLMLENEAGEVIKGEQVT
                     LQNQESSLNFKLEF"
     gene            complement(4018..4398)
                     /locus_tag="PLG24_04210"
     CDS             complement(4018..4398)
                     /locus_tag="PLG24_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24122.1"
                     /translation="MRRRRNISSWILISVIFIHFIGANTFFGMYVADNGLFTELFCEN
                     RFKPELHCHGSCAITKLMEYEENDALKSSAPHLPVPQFYSYLHQFSQQKDFDLNEIPA
                     HNFRYLQLNLKPSVTEIDHPPALV"
     gene            4814..6529
                     /locus_tag="PLG24_04215"
     CDS             4814..6529
                     /locus_tag="PLG24_04215"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HMZ24123.1"
                     /translation="MDANFSINFRFRTTILMVFVFFIFSKMPIAQPVLQLGAPVITGL
                     SQPMQIVNAGDGTGRYFVVHKGGLIKCYDKDFNFLSDFLTVTNITSSGERGLLSMAFH
                     PDYKTNGLFYVYYTGNESGKVGALTIARYKVSTGNANLADVASKKIVLEILHPRTNHN
                     GGQLHFDQAGYLFLTTGDGGGGGDPDENSQDQTKLLGKLLRMEVNTSDTPPYYSVPTD
                     NPFGNEVYCLGLRNPYRWSFDRLNGDIWIGDVGQSNWEELDHVVQSQISGTNFGWDCY
                     EGNVSYEPSGCLPSSNYKFPFHVYSLSGAQSVTGGVVYRGAKYPSLYGWYVGAEFYNT
                     DFHRVFFDGTNYTVAPVQNLGPGSIVNFGEAEDGEVYVVSLYEGEIYPLQVDEGPLPV
                     RFITLGAHRMGLTNLIEWTAAEEEGSSHYEIESCMDGTTFKKIGYIHAKASGKSGIHS
                     YIFSDIPNSVGKMYYRVKAVDVNNSYRYSDIVTVVDERSRIIVVYPTLVQYDEICVYP
                     NSINDHIILELFNTNGQLMGQRTVAPGEREVRLPVNNATPGVYYVRVTSGHTIEMHKI
                     VKPMN"
     gene            7473..8390
                     /locus_tag="PLG24_04220"
     CDS             7473..8390
                     /locus_tag="PLG24_04220"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HMZ24124.1"
                     /translation="MAKPAWTGVYPALLTPFTKDDQIDLELFGKNLKAQIDAGVSGVI
                     IGGTLGEASTLEHGEIISMLEYAKSNVAADFPVVVNVAEGSTRNAIRAAQNIQAHGAD
                     GIMLLPPMRYKSDDAETVAFFSAVATSISLPVMVYNNPVDYKIEVTLPMFEQLAKIEN
                     IQSVKESTRDVSNVTRMKNTFGDRFKILCGVDTLTLEEIALGADGLVAGLVDAFPAET
                     VAIFNLMKAGRYEEALKIYRWFLPLLELDIVPKLVQYIKLAATQTGIGSEYVRAPRQM
                     ISGTERERVLDIINRGLANRPQLPDYRNL"
     gene            8394..>9374
                     /locus_tag="PLG24_04225"
     CDS             8394..>9374
                     /locus_tag="PLG24_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMZ24125.1"
                     /translation="MIKGINHIGYEAVAGTGPSFRAFNPTIGEFFGEEFFSASEEQIG
                     KAAELAGEAFHIYKNISPEKRAEFLEAIADAILDLGDSLLERAHQESGLPLARLTGER
                     GRTVNQLRMFADILREGSWVEAVVDTAMPDRQPLPRPDIRKMLLPIGPVAVFGASNFP
                     LAFSTAGGDTASSLAAGNPVIVKVHPGHPGTNELVASAILSAARKTGMPNGVFSSIIG
                     NRIETGVQLVKHPCVTAVGFTGSYQGGMSLIKTAAERPVPIPVFAEMGSINPVVVLPG
                     IIERETEMIAGQLAASVTLGVGQFCTNPGLIFIMEGGKSEDFISILMEKMA"
BASE COUNT         2615 a         1922 c         2077 g         2760 t
ORIGIN      
        1 aatgcagcaa cagttgaggc tggaaggcga atacccattt cttttggcta caccattggg
       61 cgtaaaggga agtttcaaat tgttccgatc tgactcttcc ttcatcgaga agattattga
      121 atttggcgga caatattatt ttggctatga cagcaagctg gaagctttct ggaagcgtca
      181 gcaatctgac atcaacacgg tggatacatc gaaagtcatc gctacaaaaa aattgcctgc
      241 cagtcttgat tacaggacgg attatttcgg attgacgtac aatattcagc atctcgattt
      301 tcggctcaac ccacgcaagg gcttctcagt tgatgttcag atgggtattg gtcaacggcg
      361 aatctttaag aatgataaga tattggccat caaaggccct gaccaatcag gttttgatta
      421 cagaacactt tatgatacag taacactgcg atccacactt ttcagacctg tcattgacag
      481 caggatcttc tttccattgt tcaaacgaat ggctatcatg attcgagcca atggagctat
      541 gatagcgggt cccaagctgg tatataataa tgaaaaattc aggatcggag gcaacaagac
      601 attccgggga ttcaatgaac aatctttcta tacgacatct taccttattg ccactacaga
      661 agtcaagatg gtactggatg aattgagtgc actgttcgtc tttgcagata acggatggat
      721 taactcgacc agtaccattc ggctcaataa ttatctgtgg aatatgggcg taggtgcagg
      781 aatcaacttc ggaaccaaag taggggtttt cggattgagt attgctgttg gaaagaaccg
      841 atcaactcct ttcaacctac aagctgccaa aatccacttt ggataccaga gtttgttctg
      901 atatccaaag caaattaaag ttttgttcaa tccacgacaa ggaaaatatg gattcgatga
      961 tttattgtca gaatttgaaa atcacattag taaaaatatt gcgccccatc tttgggatgc
     1021 caaaccaatc atcgaaagtc gaataatatt tgtcaaacag attttcgacc cccagcttga
     1081 caacaagatt gatgttgcca atagaaaaat cattggacaa ggcgacgttg gccaccatat
     1141 acgccggttt ttgaatctct ccgaattccg gactgttcct gttcttgctg cagccttcaa
     1201 tgcctgcctc tgcaaagaaa ctgtttttct gatacttcaa cttagcggaa tatcccaatg
     1261 gctggatcga aggaagtatg gtctcctgtg caccctggcc gtatcggtat gatgcatcag
     1321 cctggaacaa caggtccttc aggatattat aacttacgtt catcgctgta ttcaatatcc
     1381 ctgcatattc taattgttga tataccttta ctccactggc ggtgatatac atcgggaaaa
     1441 attcatcctg gggcttgcca atgatataat cctttatata aaaataattt gctttccaac
     1501 ggacagagat tttttcagca gtataaccag aggagacttc caggctgaag gatttttcat
     1561 tcttcatgtt cggattgcct atgtagtcat agttgtcgtt tacattatac agatagaatc
     1621 cataagcctc ggaaacactt ggggcgcggt cgccataccc aatacccatc ttgtgctgga
     1681 atcgtccgtt ctttagttga agatggccgg aaaggttttt aatggatcgc aatttggttg
     1741 cctttaagtc cgggtaaaac aaatggaggc tgtttaatcc cgtctcactt cggatttcat
     1801 tgtagtgacc tccgatgcca ccctgaatgc tgaggtttaa tttgtcattg agagaaatat
     1861 tgtcttctat gttgattccg gcatagatgg tatttacatc aggccaggtc agcatataca
     1921 tatccgcctc atttggattg ttgggatgca tggtcatttc agccaatgaa ttattgaggt
     1981 gaccggacag tgtagccttg aaggtatgac gattgtaatt gcctgccaat ttggaataaa
     2041 agcctgcggt cttactccaa cccggcatat ccatccgaat aggtacaacc ggacggcgac
     2101 tatcatccat gatatgggta atggtattgt aataggcctt ggtctctata aagtcaacat
     2161 gtggcgaagg gcttcgatag ctgtgctgga gtgaagcaat ggttgcctga gccagagcga
     2221 catccatagg taagccggga tatccaacat tggttgcttt gtcgaatatg actgaggcct
     2281 ccacatgctg tctgtcatta aacttaaagc ctgatattgc cgaaagatta tatttggtaa
     2341 attgagaata caacacctcg ctactcaggc cacttctctg ccctgccttg taattttctg
     2401 catcccgata ggtaaagtct acatcggtaa aaaaattcgg actcccataa ttcaaggtcg
     2461 ctccgtatat ctgttgcata ttgttacttt caaagccggc aaaagccgaa cctccgaact
     2521 ttgggaaagt cttgaagccg gatttacgcc ttaccaagtc gatgctgcct gcaatggtcc
     2581 ctccgtgttc tgcacctgat tgtccgtcat tgatattggc tttggagaga ttggtgttct
     2641 ccacatagga tgtcacaggg tccatcttgt cggtacaggc atgatagatg cgcataccgt
     2701 ctacggtgat gacgcttcgc tcagtcgcca tgccgttgag caatggttcc caggcgtacg
     2761 caccccttct gaccatattg acagaactgg agttcttaag gtaattatcc aatgagccaa
     2821 gggccttgga gtctttcatg gaccgtgcag tagcattgga gattacaact acttcaccca
     2881 actccctgaa ctggatgcta tctaaaaatg cttcctgaag actatcctga tttacttgac
     2941 caacaacttg aaaggcagta cctaatattg cgatgaataa aattattcgt ttcatgatta
     3001 aaaaattata ataaagcata aatgcactga aaactgtgca tttatgcttt tggtcaaaat
     3061 actgacatta aaattccaat ttgaaattca agctgctttc ctgattctgt aatgttacct
     3121 gctcgccttt gattacctct ccggcttcat tttcaagcat caggttgatt ttccagtatc
     3181 ctgacatcga gaatcctact tttccatgat acaggccatc gctcaattgc tccaggtctt
     3241 tgtttccggg agcactgtga ttgcccatac cgggcattct ggggtcaacc atcatcttgt
     3301 aattgtcaac tatcggaaaa ttcccactgt catcccgttg aaaaagagcc acaacgatgt
     3361 cattgacccc gattttaggt tgagcaggtt cgaccagggc cagaaaatat ttcttaccat
     3421 cgcttcctgt ggcagaagag aatttcttgg aaaattcagt ttcggatggc aacacattga
     3481 tcttatccga tattttgtac gtctgtccat caaggttaca ggtaaatgtc aattcccaga
     3541 aattctcact gtctccgctc ggcatgatga atactatata tccttcatac agggtttctt
     3601 tgccttcgac cttggtaatg cccgaaacag ggcagctatg ctgatgagtg ttgccatgca
     3661 tattcatggt catcatcggc aacagggaga tagaggattg tttgataaaa ttgccttgtt
     3721 tatcggtcaa gcggatgaat attttattga agcctacgct tagtgtacct gagccgttaa
     3781 agacttctgc tttaatatcg ttactttcaa atgatttgac taatagcagg ccttctgttt
     3841 cattgatgtt attaattgga tttttgattt cttccttagt acaagatatg aggaaaagag
     3901 atgctatcag aaatagcatg aatattttaa tatttttcat tttatttgaa attttagttt
     3961 aataaattgt gattcgtttt catcatttga agacttttgt gaatcattaa aatttattta
     4021 aactaaagcc ggaggatgat caatttcggt tacacttggt tttaaattaa gttggagata
     4081 cctgaagttg tgcgctggga tttcattcag atcaaaatcc ttctgctggc tgaattgatg
     4141 aagataagaa taaaattgag gaacaggaag atgtggggca gacgatttga gcgcatcatt
     4201 ttcttcatat tccattaatt tggtgattgc acaggaaccg tggcagtgta attccggctt
     4261 aaacctgttc tcacaaaaca actcggtaaa taagccatta tccgctacat acatgccaaa
     4321 gaaagtattt gccccgatga agtggataaa tatcacggaa attaatatcc aggaagatat
     4381 gtttcttctc cttctcatgc gtccgcaaag ataatgactt tattccgcat attttaataa
     4441 aaattaaaaa atagttaaca aaattagatc gtctgactgt tccaataata taccgatctt
     4501 gaaagaggaa attcccatcg gaatggttag ttgtaatgct cgtattttcc ggtgcgtatt
     4561 gtgtccaggt acatcttaag aaagatatac agggccttga attttcgatg aaaaaaatga
     4621 ggtgtgtttt tgatttaaca agttaattta ttatctttgt agcattaagc ctctttattc
     4681 attagttaga cctaagtaaa taattggaac cgagagaatc cttcctttgg gattctgtcg
     4741 aattatcgaa aaaagttcac acgctgctaa gcatcattgc tctaatcttt tttaacctct
     4801 aaaggtatgt tgtatggatg ctaatttttc tatcaatttc cgcttcagga ccaccatttt
     4861 gatggtattc gtatttttca ttttttcgaa aatgcctatt gctcagcccg tattgcaatt
     4921 gggggccccg gttatcaccg gcctttctca gcccatgcag attgtaaatg ccggtgatgg
     4981 aacagggaga tattttgtag tccataaagg tggcctcatc aagtgttatg acaaggattt
     5041 taattttctt tctgattttc tgaccgtgac caacattaca agctcagggg agcgcggtct
     5101 tttgagtatg gcatttcacc cggactataa gactaatggc ttattctatg tctattatac
     5161 cggaaatgaa tcgggtaagg taggtgcact tactatagct cgctataaag tgagtacagg
     5221 aaatgccaat ttggcggatg ttgcatcaaa aaagatagtt ctggaaattt tacatcccag
     5281 aaccaaccac aatggtgggc agttgcattt tgatcaggct ggctacctgt ttctcactac
     5341 cggtgatggt gggggaggag gcgatccgga cgaaaactcg caagatcaga ctaagttact
     5401 gggcaaactg ttgcgtatgg aagttaatac ctcggataca ccaccctatt attctgttcc
     5461 aactgacaac ccattcggca atgaagtcta ctgtctggga ctgcgaaatc catatcgttg
     5521 gagctttgat aggctcaatg gcgatatttg gataggagat gtggggcaaa gcaattggga
     5581 ggagttggat catgtggttc aatcacaaat cagtggtact aatttcggtt gggactgcta
     5641 tgaaggtaat gtatcttatg aaccatccgg ttgtctgcct agttccaact ataagtttcc
     5701 ctttcatgta tattcattaa gcggagcgca aagtgttacg ggaggggtag tatatcgggg
     5761 agccaaatat ccatccctgt atggttggta tgtcggagcg gaattttata acacggattt
     5821 tcaccgggta ttttttgatg ggacaaatta tacagttgca ccggtgcaga atcttggtcc
     5881 gggatcaatt gtcaattttg gtgaggcaga agatggtgag gtatatgtag tctccttgta
     5941 tgaaggcgaa atttaccctc tccaggtgga tgagggaccc ttaccggtac gctttataac
     6001 tttaggtgca catagaatgg gcctgacaaa cctgatagaa tggactgcag cagaggaaga
     6061 aggaagtagc cattatgaaa ttgagtcctg catggatgga actaccttca aaaaaattgg
     6121 atatatacat gcgaaagcaa gcggaaaatc cggtatccat tcttacattt tcagcgatat
     6181 acccaactct gtcgggaaaa tgtattatag agttaaagcc gtggatgtca ataacagtta
     6241 caggtattcg gatatagtta cagtggttga tgagagaagt aggattatcg ttgtgtaccc
     6301 tacattggtg caatacgatg agatctgtgt ttatcccaac tcaattaatg accacattat
     6361 actggagcta ttcaatacaa atggacaatt gatgggtcag aggacggttg ctcccgggga
     6421 gagagaggtg aggctgccag taaataatgc tactcccgga gtttattatg tgcgtgtaac
     6481 ctcaggtcac accatcgaaa tgcacaagat tgtcaaacct atgaattgat tggaatatga
     6541 atgggtgatt tgagcgcttc ccgggacaga tcattcacac agcctaaagt aggctatgtg
     6601 tatagaaaga tttcagagtg acggaacaat tgagtatcct tcctacgcat ccggcatttt
     6661 gctgttttgt ttgaacgccc tttcttttca aacaagccta ttgcatttgg tcatttggta
     6721 atgttgtatt cctgagaagg catatgcaat ctgtaggcat attccttgtc actgcatcaa
     6781 cgttatttat ttcaatcatt aacatgctgg agaaatttta caggcgacct cctcctgata
     6841 cctgccttta gcacagctat tataactggt cgccctcata ttgattttca aaaatgttcc
     6901 ggtcgatgac aatgcagtct ctaataatta ccggttggat atattgagtg cacatactat
     6961 attgatctga gcgtaagtca cctttagtga tttgttcagc gaagcccttg gtgatactgt
     7021 gtataaagcg tagaagagaa tcgatgtctt cggactaccg gaccgcgaca agatatcatt
     7081 tctttaatcg aattgtttca caagagtttt gacagaatca tctgacttca atatatacgt
     7141 gattgttctc tgatgccact acgcgttgta gctttcatcc gtgctgaaaa gtcttttgat
     7201 tatccgaatg aagtggacga ggccctcgtt ctcttatttt taaattttat aagcaagggt
     7261 gagactcctg ggatatataa gtatatgctg tagccaaatt ttaccttatt tatatgtggt
     7321 taatatcccc cggatgtaaa acaataattc acccatttta ttgaataaat atataaaatg
     7381 ggttaaaata ggtaaataat cggctatcta acgtttaggt tgattttctg catcgtcata
     7441 cctttgaccc aaaataaaac agcatttaaa ctatggcaaa accggcttgg acgggcgtgt
     7501 atcccgcact tttgacaccc tttacgaaag atgaccagat cgatctggaa ctatttggca
     7561 aaaatctcaa agcacagatt gacgcaggtg tttccggcgt aatcattggc ggaaccctgg
     7621 gtgaggcaag tacgctcgag catggcgaaa tcatttcaat gctggaatat gctaaatcca
     7681 atgtcgctgc cgactttccg gtagtggtca atgttgccga aggctctaca cgcaatgcta
     7741 tccgtgcggc tcaaaacatc caggctcatg gagccgatgg catcatgtta ttaccaccca
     7801 tgcgatataa atccgatgac gcagagactg tagcattttt tagtgcggtg gcaacttcca
     7861 tctcgctacc ggtaatggta tacaataatc ctgtagacta taagatagaa gtgacacttc
     7921 ccatgttcga acaacttgct aaaatcgaaa atatccaatc agtcaaggaa tccacccgtg
     7981 acgtatcgaa tgtcaccaga atgaaaaata cttttggtga tagattcaaa atattgtgcg
     8041 gcgtggacac acttacgctg gaagaaattg ccctgggcgc tgatggcctg gtggccggcc
     8101 tggtggatgc ttttccggca gaaactgtcg ctatcttcaa tctcatgaaa gccggacgat
     8161 atgaagaggc gttaaaaata taccgatggt ttttgccatt gctcgagctt gatattgtac
     8221 ccaagctggt acagtacatc aaactggcag ccactcaaac aggtatcgga agcgagtatg
     8281 tccgcgctcc ccgccagatg atttccggta cagagaggga aagagtgctc gacatcatca
     8341 atcggggatt agctaatcga cctcaattac ctgattatag aaatctgtaa aacatgatta
     8401 agggaatcaa tcatatcgga tacgaagccg tagcagggac aggcccttcc ttccgcgcct
     8461 tcaatcccac gatcggtgaa tttttcggag aagaattttt ttctgccagt gaagaacaga
     8521 ttgggaaagc cgcagaactg gccggggaag ccttccatat atataaaaat ataagtcctg
     8581 aaaagcgtgc cgaatttttg gaagcaattg ccgatgcgat acttgacctc ggagatagcc
     8641 ttcttgagcg ggcacatcag gaatcaggcc tgccactcgc gcgactgacc ggcgaaagag
     8701 gcaggacggt caaccaattg cggatgtttg ccgatatatt gagggaaggt tcctgggtgg
     8761 aggcagtggt agataccgcg atgcccgaca ggcagccact tccaagaccg gatatccgaa
     8821 aaatgttact acctataggt cccgttgcgg tatttggtgc aagcaatttt cccctggcat
     8881 tttcaactgc cggaggagat accgcaagtt cactggctgc cggcaatccg gtaatcgtta
     8941 aggttcatcc gggccatccc ggcaccaacg aactggttgc atcggcgatc ctatcagctg
     9001 cccggaagac aggtatgccg aatggtgttt tttcttccat catcggcaac cggattgaga
     9061 caggtgttca gcttgtcaaa catccttgtg ttactgctgt gggctttacc ggttcttatc
     9121 aggggggaat gagcctgata aaaaccgctg ctgagaggcc tgtaccgatt cctgttttcg
     9181 ctgagatggg cagcatcaat ccggtcgttg tccttcccgg tataatagaa agagagacag
     9241 aaatgatcgc agggcaactt gctgcttctg ttactttggg cgtgggtcaa ttttgcacca
     9301 atccgggttt gatcttcatc atggaaggag gaaaatccga agacttcatt tcaatactta
     9361 tggaaaaaat ggct
//
LOCUS       DAOQQN010000074         9355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2750_length_9355_cov_4.717957, whole genome shotgun sequence.
ACCESSION   DAOQQN010000074 DAOQQN010000000
VERSION     DAOQQN010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9355
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2750_length_9355_cov_4.717957"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PLG24_04230"
     CDS             <1..762
                     /locus_tag="PLG24_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24126.1"
                     /translation="MKSSWAWLDMMIHHSETARPFDDIITEMAAAIPAFEGIKSLAPN
                     ADFRIGTQKIPRESHRYSGRTSMNASVSVHEPKPPVDVDSPLSYTMEGFHGKAPSSDI
                     PFFWSPGWNSVQSINKYQVEVGGPLHGGNPGLRLIEASEDGNASFESHEYQGKGTFEV
                     LPAWHIFGSGELTALSPAIAKRIPEATASISDAGLAKAGLTGSTNIRVRYNGREIVLP
                     VNIDNSLPENVVMIPYGLKETAGVHYPFFGEIESV"
     gene            800..1756
                     /gene="nuoH"
                     /locus_tag="PLG24_04235"
     CDS             800..1756
                     /gene="nuoH"
                     /locus_tag="PLG24_04235"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005151242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HMZ24127.1"
                     /translation="MNETLFHILVVVGVLLGTVTIAAALIYVERKALALWQQRHGPNR
                     VGPFGLFQVVADVIKIFFKEDWIPPFVNKKVFIIAPTIIMLSILMSFAVVPFGPGLVV
                     ADMNIGLLFFLAMSSLGAYTIILAGWASNNKYSLLGGVRGAAQMISYEVFMGLSLMGI
                     VIQAGSFNLTDIVNAQKDVWFVIPQFLGFVIFLIAGIAETHRLPFDLPEAESELIAGF
                     HSEYSGMKFGMFFVGEYLGITLVSCLITVLFFGGWLGPAFLPPIVWFLLKMFCFILFF
                     ILLRATLPRPRYDQLMSYGWKLLLPLALLNVLVTGAIVLFMQ"
     gene            1770..2285
                     /gene="nuoI"
                     /locus_tag="PLG24_04240"
     CDS             1770..2285
                     /gene="nuoI"
                     /locus_tag="PLG24_04240"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HMZ24128.1"
                     /translation="MFSDIRSLWMVFLKMFKKKVTIQYPEQKPYLTPRWRGRIILSRD
                     PDHNERCVACNLCAAACPTDCISLQATEDEEGRRYPEYFRINFSRCIFCGLCEDACPT
                     YAIQLTPDFEMGEYYRNNLVYEKEDLLIDGMGKYHGYSYYQQAGVAVGVKPKGGGENE
                     SAPVDVRTLLP"
     gene            2305..2841
                     /gene="nuoJ"
                     /locus_tag="PLG24_04245"
     CDS             2305..2841
                     /gene="nuoJ"
                     /locus_tag="PLG24_04245"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HMZ24129.1"
                     /translation="MNIIFYIASIVALIATIRVVTHKDAVHALLYLVVSLLAISVIFF
                     IVGAPFVAALEVIVYAGAIMVLFVFAIMMLNLGKDSVKQEASLFSPRAWIVPSILCLV
                     LLVEIIYVLAGGGLPGSNVKIISAQEVSNTMFKGYVLAVELTGMLLMAGIVGAYHIGK
                     EKRKEYHRYLQINEDENE"
     gene            2834..3142
                     /gene="nuoK"
                     /locus_tag="PLG24_04250"
     CDS             2834..3142
                     /gene="nuoK"
                     /locus_tag="PLG24_04250"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004881318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HMZ24130.1"
                     /translation="MSDLPIQTGMLVAGILFLAGLYGVLTRRNVLFMLMSVEIMFNAA
                     GLAFVLAGAKHGQADGQVMLIFILAMAAAEVAVGLALILQMYRLNKTVDTDAINQLRD
                     "
     gene            3164..5071
                     /gene="nuoL"
                     /locus_tag="PLG24_04255"
     CDS             3164..5071
                     /gene="nuoL"
                     /locus_tag="PLG24_04255"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HMZ24131.1"
                     /translation="MNIAEILWLIPFFPLAGFLILAFFGKKIQRSLVPVIGSGVIGIS
                     ALITIVAGISFMNNSEPGAVIEQHLWTWFRIGDFHPEITLHLDGLSLIFIFVITFVGF
                     LIHVFSSEYMKHDDGYSRFFAYMNLFVCSMLILVLANDLLLLYLGWEGVGLCSFLLIG
                     FYYGDTKDAEKNALAARKSFLVTRIGDTSMILGFFLLVKTFNTLNISEILQAAPSVWE
                     TDSTTAIWAAALLLGGALGKSAQLPLQTWLPDAMAGPSPVSALIHAATMVTAGVYLLT
                     RTYVLFELAPTVQMAVAIIGAATLFIASCSALAQDDIKRVLAYSTISQIGYMFLACGV
                     GAWSAAIFHFMIHAFFKALLFLGAGAVILALHHEQNMFKMGGLRKKLPVVFWTFLFGS
                     AALASIPLITAGFYSKDAILWYSFAGEKGSIWLWLAGVVSAFLTALYTFRMVFLTFFG
                     EQKTEVSHQPGLAIKVPLIILGILSLVAGFIELPHNFGHFAPFSEFLDKVLSPVKLVA
                     SPAIGEGILQLVAGGLALMGLFLAWRMYGKANATQQQFGSPAAGGTFNFFYNGWNFDK
                     LYDILFVKPLVWLSRIDEKDFVDSIYKGIGRLNIEMNGALSRTQNGKLRYYLLGLGLG
                     AMIVLTLILVK"
     gene            5068..6645
                     /locus_tag="PLG24_04260"
     CDS             5068..6645
                     /locus_tag="PLG24_04260"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HMZ24132.1"
                     /translation="MILVTLLAILMGGGILAWVAGTYNKTASRWISLVALLIDLGITA
                     GIWMNGVTGTTAAEGADWITSVKYDWIPNFGMTFHLAMDGISLVMTSLTFFLGAIAVL
                     VSWKSIEKNVGFFHFNILWLLAGITGVFLAMDMMLFYFFWEIMLIPMYFVMVIWGHKI
                     SRYAGWKFFLFTQISGLMMLISIIGLFIYHGVQTGSYTFNYFDLLASVPGGPVAFWLM
                     AGFLLAFFVKLPALPFHSWLPDAYTESPSAGSIIIAALMSKTAAYGLIRFVLPLFPEP
                     AHDFAVPALMLGVVSILYGAKLAYAQRNLKRLIAFSSFSHMGFILIGVFSFNMLALQG
                     VMMQMVAHGLSIAALFIISEFIYERIGTLDLLEMGGFWRSMSNMGGFTLVFVMASLGL
                     PGLANFVAEFMILAGAWQFDVLMAVLATLGLIVSVTYSLRILQKVFHQNASVKAGELN
                     DLNIREWVVMGCLTIAIIWLGLFPQEVVKVAQPAFDRMGNNMHQNKSGEIGMNDTNLE
                     LMQRRNIENIENGGTAW"
     gene            6639..8054
                     /locus_tag="PLG24_04265"
     CDS             6639..8054
                     /locus_tag="PLG24_04265"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HMZ24133.1"
                     /translation="MVTLNELKFLLPEVTLALGAVIVMLAIAAKRNHLLSNILTLAVF
                     GVSFFFLATLPAEDLLVGKLFVIDPTSRFMTGLMLCSAFIITLYSFPYFKIQNETKEE
                     YYILLILATLGSVCMTMSDHFVSFVISLEVLSVSLFGLIGYLKKSTISIEAAIKYLIM
                     AAVATAFILFGFALIYSVTGKMDLMGLSESFSNGQIDNMAVTGLALVIVGFGFKMALT
                     PFHLWTPDIYSAAPSPVAAFVATISKGAAFIFLMRLFYSTGGLEHFGVWITFVLVATA
                     SMFIGNWLGLRQQNLKRLLAYSSISHLGYLMVGFLAVSHAGIKASVFYLVIYFTSILA
                     AFGMITYLTGKKGEPTMVEDYRGLFWTKPWLAAFFTLVMLSLAGIPLTAGFMGKFYLL
                     TAGMAYGSLFLLIVLVVSTTIGLYYYLRVVAVMLSRSEAQIGEHIGSNSFVLKAVFAV
                     LFVLILWMGVYPNTFFSLLGM"
     gene            complement(8100..8693)
                     /locus_tag="PLG24_04270"
     CDS             complement(8100..8693)
                     /locus_tag="PLG24_04270"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HMZ24134.1"
                     /translation="MFTGIIEALGKVISDKREGEKIHYRLTCPFTKELKLGESISHDG
                     VCLTVTDITDKDYAVTAIDTTLKITNIDWKEGKLVNLERAMSANSRFDGHIVQGHVDT
                     VGIVKEILHTEGSVQIEISFDSRFAKNIIDKGSITVNGISLTIVKTSNDSFSIAIIPH
                     TWSHTNLHQLKEGDRVNLEFDVIGKYVAKILSAQGVH"
     gene            complement(8748..>9355)
                     /locus_tag="PLG24_04275"
     CDS             complement(8748..>9355)
                     /locus_tag="PLG24_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ24135.1"
                     /translation="SPTLKITNDCMAENPSPSIVNKLTWNNPDEVCDTPGDTKGYRIY
                     VIHDKVDEPELIFETNDPKLISLDYASEEYGLAGCYYIVAFDSVFNESPKSNIVCIEN
                     CPSYRLPNAFSPNNDGHNDVFKPYPYRFVNKVEFKVFNRWGNVIFETTNPNINWDGNN
                     RAGNKVPDGTYYYTCKVFETIETMTGIENTIILTGYIELIR"
BASE COUNT         2341 a         1751 c         2167 g         3096 t
ORIGIN      
        1 atgaagagca gttgggcatg gcttgatatg atgattcatc attcggaaac agcaagaccg
       61 tttgacgaca tcatcacaga aatggctgct gctatacctg ccttcgaagg aatcaaatcg
      121 ctggcaccta atgctgattt tagaatagga acgcagaaaa ttcctcgtga atcacataga
      181 tatagtggaa ggacatccat gaatgcttct gtaagtgtac atgagcccaa gccccctgtt
      241 gatgtggact ctccattgag ctatacgatg gaaggattcc atggtaaggc accttcctct
      301 gatattccgt ttttctggtc tcccggctgg aattctgtac aatctatcaa caaatatcag
      361 gtggaagttg gtgggccctt acatggtggt aatccgggat tgaggttgat tgaggcgtct
      421 gaggatggaa atgcttcgtt tgaaagtcat gaatatcaag gtaaaggtac ctttgaggta
      481 ttaccggcat ggcatatttt cggatccggt gagttgacag cattgagccc tgccatagcc
      541 aaacggatac cagaagcaac cgcgtccatt tcagatgcag gattggcaaa agccggtctc
      601 acagggtcaa caaatataag agtgcgatac aatggtagag aaattgtttt gccggtgaat
      661 attgataata gtcttccgga aaatgtggta atgatacctt atggactgaa ggaaacagca
      721 ggtgtccatt atccgttttt tggagaaatt gaaagcgtgt aataaaaagg gataagttaa
      781 aatttaataa tcaagggata tgaacgaaac cctgtttcac atactggttg tagtcggcgt
      841 actgttgggt acggtgacta tcgctgctgc tttgatatat gtcgaaagga aggcgcttgc
      901 attatggcaa caacgccatg gccctaatag agtggggcca tttggactct tccaggtcgt
      961 tgctgacgta attaagatct tcttcaaaga agactggatt ccaccttttg tcaacaaaaa
     1021 ggttttcatc attgcaccaa ccattattat gcttagtata ttaatgagtt ttgctgtagt
     1081 tccatttggt ccgggtttgg ttgtggcaga tatgaatata ggccttttgt ttttcctggc
     1141 aatgtcatct cttggtgctt acacaatcat acttgcaggt tgggcttcca acaacaaata
     1201 ctctctgttg ggaggcgtac gcggagcagc acaaatgatt tcatatgagg tgtttatggg
     1261 tttatcactc atgggaattg taattcaggc aggttcattc aatttgaccg atatagtcaa
     1321 tgcgcagaag gatgtatggt ttgtcattcc tcagtttctt ggctttgtga tctttttgat
     1381 agcgggaatt gcagagacac accgattgcc cttcgacctt cctgaagcag aaagtgagtt
     1441 gattgccggc ttccactctg aatattcagg aatgaagttt ggaatgtttt tcgtaggtga
     1501 atacctgggc attacacttg tgtcctgcct cattaccgtt ttgttctttg gtggatggct
     1561 agggccggcg tttttgcctc caattgtttg gtttctatta aaaatgtttt gtttcatatt
     1621 gttctttatc ctattgcgtg ctactttacc cagaccgcga tatgatcagt tgatgagcta
     1681 cggctggaag ctgttattgc ctcttgcttt gctcaatgta ctggtgaccg gagcaattgt
     1741 acttttcatg caataaatat ttaagaatca tgtttagtga tattcgttct ttatggatgg
     1801 tctttctgaa aatgttcaag aaaaaggtta ctatacagta tcctgaacag aagccctacc
     1861 ttacaccacg gtggcgaggt cggatcattc tttcgcgtga tccggatcat aatgaaagat
     1921 gtgtagcatg taacttgtgc gctgctgcat gtccgacaga ttgtatttcc ttacaggcta
     1981 ctgaagatga ggaaggaaga agatatccgg aatatttcag gattaatttt tcaaggtgca
     2041 tattttgtgg actttgtgaa gatgcttgtc cgacctatgc aatacaattg acaccggatt
     2101 ttgagatggg tgaatactat cgtaataatc tggtctatga gaaggaggat ttgttgattg
     2161 atggaatggg taaatatcat ggttacagtt attatcaaca ggcaggagtg gcagtaggcg
     2221 tcaaaccaaa aggcggcgga gaaaatgaaa gtgctcctgt cgatgtaagg actttattac
     2281 cataaaacta ttaactagct tttcatgaat ataattttct atatagcatc aattgttgcc
     2341 ttgatcgcca ccatcagggt tgttacacac aaagacgcag tgcacgcttt gttgtatctg
     2401 gttgtttctt tattggcgat cagtgtgatt ttttttattg tcggagctcc cttcgttgct
     2461 gcgttggaag taattgtata tgccggcgcc atcatggttt tgtttgtatt tgccatcatg
     2521 atgcttaatc tgggtaagga ttctgtcaaa caagaagcaa gtctgttcag ccccagggca
     2581 tggatagtgc cttctatttt gtgccttgta ctgttggttg aaataattta tgttcttgca
     2641 ggaggagggt tgcccggtag caatgtaaag attataagtg ctcaggaagt ctccaataca
     2701 atgtttaaag gctatgtgct ggcagtggag ttgacaggta tgctattgat ggccggcatt
     2761 gtgggtgcat accatatagg aaaggaaaag agaaaggaat atcatcgtta tctacaaata
     2821 aacgaagacg aaaatgagtg atttaccgat tcagacagga atgctggttg caggtattct
     2881 gtttttagcc ggcctttatg gcgtcttaac gaggcgaaat gtattgttca tgctgatgag
     2941 cgtggagatt atgttcaatg ctgcgggact tgcttttgtg ttggcaggag cgaagcatgg
     3001 gcaggcggat ggccaggtca tgctgatatt tatccttgcc atggcagctg cagaggtagc
     3061 tgtgggattg gcattgatac tacagatgta tcgcctcaac aagacagtgg atactgatgc
     3121 tatcaatcaa ttacgggatt aaattaaaag aaatagttag cttatgaata ttgcggaaat
     3181 actttggcta ataccttttt ttccattggc aggatttctt attctcgcct ttttcggtaa
     3241 aaaaattcaa cgaagccttg ttccggtgat tgggtccggt gtgatcggca tatctgcact
     3301 catcacgata gtcgcaggca tcagctttat gaataattcc gaacccggtg cagttatcga
     3361 acaacatctg tggacctggt ttcgcatcgg agattttcac ccggagataa ccctccatct
     3421 tgatggccta tctttgatat ttatctttgt gataaccttt gtaggattcc tgatccatgt
     3481 gttttcatct gaatatatga agcatgatga tggctattcc cgtttttttg catacatgaa
     3541 tctttttgtg tgcagtatgc ttattctcgt ccttgccaac gacctattac tgctgtatct
     3601 gggatgggaa ggcgttggac tttgtagttt tcttctaatc ggattttatt acggagacac
     3661 gaaagatgct gaaaagaatg ccctggctgc ccgcaaatcc tttttagtca ccagaatagg
     3721 cgatacatcc atgatcctgg gtttcttcct gctggtaaaa actttcaata ccctgaatat
     3781 ttccgaaata ttgcaggccg ctccgtcagt ttgggaaaca gatagtacaa cggcaatttg
     3841 ggcagctgca cttttattgg gaggagcatt aggcaagtca gcccaattgc cccttcagac
     3901 ctggcttcct gatgcaatgg caggaccttc accggtgagt gccttgatcc acgccgcaac
     3961 aatggttaca gccggggtat atttactgac acggacttat gttctttttg aacttgctcc
     4021 aaccgtgcaa atggctgttg ctatcattgg cgcagcaacc ctattcattg cttcatgcag
     4081 tgctctggct caggacgata taaaacgtgt attggcatac tcaaccatca gccaaatagg
     4141 ctatatgttc ctggcatgtg gcgttggagc atggtcagcg gcaattttcc attttatgat
     4201 acacgctttc ttcaaggcat tgctcttcct tggtgccggt gctgttattc tcgccttgca
     4261 tcacgaacaa aatatgttca agatgggcgg cttgagaaaa aagctaccgg ttgtattctg
     4321 gacttttctt ttcggatcgg cggcattagc ctccattcct ttgattactg caggattcta
     4381 cagtaaagat gcaattcttt ggtattcatt tgctggtgaa aaaggtagta tatggctttg
     4441 gttagcaggt gttgtgtctg cttttctgac ggcactctat acctttagga tggtgttcct
     4501 cacattcttt ggagagcaaa aaactgaagt atcccatcaa ccgggattgg cgattaaagt
     4561 accacttatc attctgggaa tcttgtctct cgtggcagga tttattgaac taccgcacaa
     4621 cttcggtcat tttgcacctt ttagtgaatt cctggataag gttttatcac ctgtcaaatt
     4681 ggtcgcatct cctgcaatag gagagggtat attgcaactt gtcgccggcg ggcttgcttt
     4741 aatggggctg tttttagcat ggcgcatgta tggtaaagcg aatgcaacac aacaacaatt
     4801 tggttcacct gcggcaggag gaactttcaa tttcttctac aatggctgga attttgataa
     4861 attatatgac atcctttttg ttaagccttt ggtatggctt tccaggatcg atgaaaagga
     4921 ttttgtggac agtatttaca aaggtattgg caggctaaat atcgagatga atggtgcgtt
     4981 gagtcgcaca cagaatggta agttgaggta ttacctgctt ggattgggat taggagcaat
     5041 gatcgtactc acattaattt tggtaaaatg atattagtaa ccctattggc aattttaatg
     5101 ggtggcggca tacttgcttg ggttgccggt acttataata agacggcttc gaggtggatt
     5161 tcactggtgg cgttgctgat agatctcggt attacagccg gtatctggat gaatggtgta
     5221 acaggcacta cagcagcgga gggagccgat tggattactt cggttaagta tgactggatt
     5281 ccaaatttcg gtatgacatt tcatcttgcg atggatggca taagccttgt catgacatca
     5341 ctgacctttt tcctgggcgc tattgctgtg cttgtctcgt ggaaatcgat agaaaaaaat
     5401 gttggctttt tccactttaa tattctttgg ttactggcag ggattacggg tgtctttttg
     5461 gctatggata tgatgctctt ttatttcttc tgggaaatca tgctcattcc tatgtacttt
     5521 gtaatggtaa tctggggaca taagatcagt cggtatgccg gatggaaatt cttcttattt
     5581 actcagatca gcggtctaat gatgcttata tccatcatag gcctgtttat ttatcatggt
     5641 gttcagaccg gttcatatac ttttaattat tttgatttat tagcctcagt acccggtggg
     5701 cctgttgcat tttggttaat ggctggattt ctactggcat tttttgttaa gttgcccgct
     5761 ctacctttcc atagctggtt gccggatgct tatacagaat ctccgtcagc aggaagtatt
     5821 atcattgcag cactgatgtc taagacggct gcttatggat tgataagatt tgttttgcca
     5881 ttatttccgg aaccggcaca tgattttgct gtcccggcat tgatgttggg agtagtttcc
     5941 atactatatg gagctaaact tgcctatgca caacgaaatc tgaaacgttt gatcgcattt
     6001 tcaagcttta gccacatggg ctttatttta attggtgttt tctcattcaa tatgcttgct
     6061 ttgcagggag ttatgatgca aatggtagct catggtttaa gcatcgccgc acttttcatc
     6121 atatctgaat tcatttatga gagaattgga accttggatt tgcttgaaat gggaggcttc
     6181 tggaggagca tgtccaatat gggtggattt acattggtat ttgtcatggc atctctcgga
     6241 cttccgggct tggcaaactt cgtggcagaa ttcatgatac ttgcaggtgc ctggcagttt
     6301 gatgtactca tggctgtcct tgccacactt ggcttgatag tttcagttac ttattcactt
     6361 cggattttgc aaaaagtttt ccatcaaaat gcttcagtga aagcgggaga actaaacgac
     6421 ttaaatataa gagaatgggt tgtgatgggg tgtcttacga ttgcgatcat ctggctggga
     6481 ttgtttccac aggaggtcgt aaaggtggcc cagcctgctt ttgatagaat gggaaataat
     6541 atgcaccaga ataaatccgg tgaaatcgga atgaatgata caaatctgga attgatgcag
     6601 agaagaaata ttgaaaatat tgaaaacgga ggtacagcat ggtaacattg aatgaactga
     6661 aatttttgct gcctgaggta acactggccc tcggtgctgt aattgtgatg ttggcgattg
     6721 cagccaaacg taatcacctt ctgagcaata tcctgacatt agctgtattt ggcgtttcat
     6781 ttttctttct ggcaaccttg cctgctgagg atcttttggt tggtaagttg tttgtgatag
     6841 acccgacaag caggttcatg accggtttga tgctatgttc agcttttatc atcaccctat
     6901 actcatttcc atatttcaag atacagaatg aaacaaaaga agagtattac attctgttga
     6961 ttttggcgac tttgggttct gtatgtatga cgatgagtga ccattttgtt tcattcgtta
     7021 tctcattaga ggttctcagt gtttccttgt ttggattgat tgggtatctt aagaaatcga
     7081 ccatttctat cgaagcggct ataaagtacc tgattatggc tgcagttgca actgctttca
     7141 tattgtttgg atttgcattg atttattctg tgacgggaaa gatggatctc atgggactct
     7201 cagaatcatt ttccaatggg cagattgata atatggctgt tacaggattg gcactggtca
     7261 tcgtgggatt tggctttaaa atggcgttga ctcctttcca tctctggaca cctgatatat
     7321 attccgctgc tccttcacct gttgcagcat tcgttgcaac catcagtaaa ggtgctgcct
     7381 ttattttctt gatgcgacta ttttacagca ccggtggcct ggagcatttc ggtgtatgga
     7441 ttacattcgt gctggtagca acagcttcga tgtttatcgg taactggctt ggtttgaggc
     7501 aacagaattt aaagagattg ctggcttatt cttcaatttc ccatttgggt tatctgatgg
     7561 tcggttttct tgcggtttct catgccggta tcaaagcttc cgtattctat ctggtgattt
     7621 actttaccag tattcttgct gcttttggta tgattaccta cctgaccggt aaaaaaggag
     7681 aaccaaccat ggtcgaggac taccgtggtt tgttctggac aaaaccctgg ctagctgcat
     7741 tttttacatt ggttatgctt tcgcttgcgg ggataccact tactgccggg tttatgggta
     7801 agttttacct tcttacggcc ggtatggctt atggatcact atttttattg attgttctgg
     7861 tggtatctac aaccattggt ttgtattatt acttacgcgt agtggctgtt atgttatcac
     7921 gttctgaagc acagattggt gagcatatcg gaagtaattc ttttgtttta aaggctgtat
     7981 ttgcggttct ctttgtttta attctatgga tgggcgtata tccgaataca ttctttagtt
     8041 tactgggcat gtaatcaaga atctctttcg tttttgtcct gttggctgtc aaaaaggtat
     8101 tagtgtactc cttgtgcaga taaaattttt gctacatatt ttcctattac gtcaaattcc
     8161 agattgaccc gatctccctc ttttagttga tgtaggttgg tgtgtgacca ggtatgaggg
     8221 ataattgcaa tcgagaagga atcattggat gttttgacta tggtcaagct gatgccattt
     8281 accgtaatac ttcctttatc aataatattc ttggcgaatc tgctgtcaaa gctgatttcg
     8341 atctgaacag aaccctctgt gtgcaatatt tctttgacta tcccaaccgt atctacatgg
     8401 ccctggacaa tatggccatc gaatcgactg ttggctgaca tagcacgttc aagatttacc
     8461 agcttaccct ctttccaatc tatattcgtt atcttcagtg ttgtatcgat ggctgttact
     8521 gcgtaatcct tgtcagtaat atcggttacc gtaagacaaa ccccatcatg actgatgctt
     8581 tcaccaagct ttaattcttt tgtgaaggga caggttaacc gataatgtat tttttcgcct
     8641 tctctcttat ctgaaataac tttgccaaga gcttctatga tacctgtaaa catgtttttg
     8701 aatttttcaa accattgata attttgaatg aagtaaagtt tggtatttta tctgatgagt
     8761 tcgatataac ctgtaaggat aatagtattt tcgatcccgg tcatagtttc aatcgtttca
     8821 aatactttgc atgtataata ataggttcca tcaggtacct tatttcccgc tcggttgttt
     8881 ccgtcccaat tgatattggg atttgttgtt tcaaagatta cgttacccca tcgattgaac
     8941 accttaaatt caaccttatt gacaaatcta tacggatacg gtttgaacac atcattatga
     9001 ccgtcattat ttggggaaaa agcgtttggg agccgataac ttggacaatt ttctatacaa
     9061 acaatgttac tcttagggct ttcattaaat acggaatcaa aggctactat gtagtaacag
     9121 ccggccaggc cgtattcttc acttgcataa tccaggctaa tcagtttggg gtcatttgtt
     9181 tcaaatatta gttccggttc atcaacttta tcgtgaatca cgtagatgcg gtagcctttg
     9241 gtatcgcccg gtgtgtcgca aacttcatca ggattgttcc acgtaagttt gttgacaatc
     9301 gaaggagagg gattttcagc catacaatca ttggtgattt tgagtgttgg gctac
//
LOCUS       DAOQQN010000075         9322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2771_length_9322_cov_5.572677, whole genome shotgun sequence.
ACCESSION   DAOQQN010000075 DAOQQN010000000
VERSION     DAOQQN010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9322
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2771_length_9322_cov_5.572677"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PLG24_04280"
     CDS             <1..287
                     /locus_tag="PLG24_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMZ24136.1"
                     /translation="LSHDPSHWDFEVIKEHKDIDLTLSGHTHGFQFGVEIPGWFRWSP
                     SRFVYKQWAGLYQKGRQYLYVNRGFGFLGYAGRVGILPEITTLVLTGKKE"
     gene            413..2203
                     /locus_tag="PLG24_04285"
     CDS             413..2203
                     /locus_tag="PLG24_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ24137.1"
                     /translation="MKAWIAFLLYFLLLGNCHILKAQAINDDCFSATELTNVRSWCSS
                     NGFFNNFSATPSGYGPATCFSSSGHDVWFSFTAIATDITILVKPGSLQKPEIALYAGI
                     CQGTIGELNCKSNLVTGGALELYKGGLFPGTVYLIRVQGEDASEGTFDLCLDNYFPPV
                     KPGSDIITASVLCDKSSFIVQNVTGSGSVPNEFKDAPCLADGFTNSETNSTWFKWTCK
                     DAGSLTFVLTPLNAPDDLDFALYELNGDLNTTNRTLLRCEAASCKGQTGLKEGATDIS
                     EPPNCDKGQDNWLAPVQLEAGKNYLLGINNFTIGGNGFRIEFGGTSTFEGPVADFEIL
                     SDTVFCREEQIGIKDLSHNSTGTIEKIQWIFGQDATPSIDSIQGDKTISYLKRGQKFI
                     VQTVTSDKGCVVSTIKPVEIQCCGPLHEMNLYSDTTITLGDSVDLHAEAFLEGNQIFY
                     FWEPPGLIQCQGCPANNIKTFHDQVIVVKATDQYNCEAKDSIQIRVDVKRPIFFPNVF
                     SPNYDGINDRFTGYGNFVVRTIDYLRIFNRWGALVWEGRNIPANDEKYGWDGTFKGRE
                     VNPDVYVYIAKVTFIDDQSFIYEGSITVVR"
     gene            2157..4007
                     /locus_tag="PLG24_04290"
     CDS             2157..4007
                     /locus_tag="PLG24_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ24138.1"
                     /translation="MINHLFTKVLLPWSGRLLRIVSVLALFLWPANLVFCQTINDECK
                     KAIFIPNVKEYCSGDTTFSFLGSTPSNNGFSLCQTDNGNDVWFSFHVTATDVVIKILC
                     KEANDLHASLYSGDCGSLNEETCGRREAGQNFLQLYKGGLVPGTRLYLRVDSENGTNL
                     KFGLCINNFFSPALDGSDCKTANLICSKESFTVRNVQGFGEVNELGNAPCFSHANESN
                     SIWFRWICRDPGTFTFKLIPLKEADDIDFVVYKVRGDAFGCDLELVRCMATGISPVNC
                     TNYPNCCGSTGLRDGETDESEGAGCGPDRNNFLKPLDMESGEVYAILINNYTSQNEGF
                     DFNMGGTATLKSLQADIDIIKPDALCREESLEVKDKIINDLGTIKSLLWTFTPDASIS
                     TSQGAGPVSVHFDRSGYKTISLIVENDAGCKAYAQDTVFVFCCGGTLRADILGNTFVD
                     YGEMAEFGVQFVLEGEDVKYQWTPAGQVNCDTCSLITLDPVYEDFPLWVTVSDENGCK
                     AENRVFIKTGKPEVFAPNIFSPNHDGINDRFMLSFAEGIHRIRSLRIYNRWGGLVYEG
                     RNMDKSDADGGWDGRFKDRDAAPGTYVYYAELETRGGKTMVIKGGLTLIR"
     gene            4047..4613
                     /gene="lptC"
                     /locus_tag="PLG24_04295"
     CDS             4047..4613
                     /gene="lptC"
                     /locus_tag="PLG24_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HMZ24139.1"
                     /translation="MNISFRTFLGLCLLALSFQACENDLAEVRRVTGVSVSGSYEEGV
                     DGEILYSDSARLKVVIKFDTLIRILDQSDPRDIFPRGLIVSFYDDNGQISSILTANRA
                     ERIVRKNMVVATDSVHLVSRNGEELLTNELIWDELKKEIYTNRFVRLTRPGEIIYSFG
                     FRSNQDFTKYELNALSGRLKKDNLDKDF"
     gene            complement(4652..5887)
                     /gene="porV"
                     /locus_tag="PLG24_04300"
     CDS             complement(4652..5887)
                     /gene="porV"
                     /locus_tag="PLG24_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HMZ24140.1"
                     /translation="MKKFIQFLVFAITLFMTSGATAQSSGTCVESPIGSNHWIEPDGS
                     PCRNSISTVVSFLRIVPDARTSGMGSVGLATSPDANAMHFNAAKLASSTKDGGLSMSY
                     VPWLKGLGLNDIFMAYLTGYYKFSKKEAIAASIRYFSLGDFTFTDTQGNIIGYGKPNE
                     FEIAVAYSRALGDKLFGALTGKFISSNLATGQQLNGVDIQTARTGAADISIFYRTPLK
                     TNTKSNLALGVAITNLGGRISYTKASPVRDLLPANLGIGMAWDVDLDQYNRLTLGVDF
                     NKLLVPSPDPALTDDNNNGVPDYREKALIPAIFGSFSDAAGGFKEELRECMFGIGAEY
                     WYNDQFSIRAGYQYESPLKGNQKYFTLGLGLKYTTFGLNLSYLVPSGNTRTALDNTLR
                     FTLMFDFGTFEVDDDPNIK"
     gene            complement(5981..>9322)
                     /gene="porU"
                     /locus_tag="PLG24_04305"
     CDS             complement(5981..>9322)
                     /gene="porU"
                     /locus_tag="PLG24_04305"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HMZ24141.1"
                     /translation="TYKFRSADAGIYRIDNQFMADLGINTSGIDPSKIKIFTTNPGPL
                     PEKNNLPRTDDLAEVAIQVIGGDDGKWDASDYILFYSPGPHRWNKESDSTFTRVTNIY
                     EDYKYFFIQVEGAPGKRIVLKDGGQAFDVESDIYDHIEHYEVDKVNLLREYAKIGGGQ
                     LWLDELITGSNRTKDLSGLLKVDNLVAGETGYVKMAFAVRSKSRSVISMIADNQAAEI
                     QFETTRIEDSESRVASYNSKKSAFTQAGGPLKISFDFPQVATESLGWMDYVEVNIKRY
                     LSKPQSAMAFRLSNPGITGNIRYNLTGLSSTDAVWDISDLFNVTQCKISPTGGSGSIV
                     APYPQQREFICFGTNDIKTPEKIGLVTPQDIHGMATPDMVIIYHAKFAEEARNYANHR
                     SSISGIDVAIVDVDHIRNEFSGGGQDPTGIRDMARMFYYRNPEKFRYILLLGDGSYDY
                     RGLTFNATSKKENSNYVPVYEFPRAILEPIRSIPSDDYFGMLDEEDGEIEKGTIDVYV
                     GRFPVYDKSQAQSIINKIISYEKNKDSFGDWRNYLMLISDDWDNVNSDDFIVQSEKIF
                     NKIQSSNPGINFSKIYLDIFKQESNLGGQSYPTAVSQMNDQYNKGVLIANYVGHGGVD
                     GLAQERIIDRLTLNGMKNSNRLPLLITGTCSFATYDDPDITSVGKLCITKPDAGMIGL
                     MTTTRSVYITPNEVFINKLFQVIYQKENGRYLTNGEILAKAKNLSSGADRLAFVLLGD
                     PAMTLAFPRENILLETVNGAPIDTAKPDTIKAMELVSLAGSVRAEDGRVIEDFNGTLT
                     LTVFDKAYTLYSRGNDGNGANYPASFQNNILFKGKATVTAGKWNISFYLPKDINYAID
                     YGKLSFYADNGIYDAGGYSHQVKIGGESSNPITDNQPPVIDLYMNTLNFKSGEIVNTT
                     PLFIARLSDDYGINVSSSSIGHEIVSTLNNDATKEKIMNDFYVADKDEYNKGEVRYQF
                     DQLDPGNYTLRLRAWDISNNKAEAEIEFIVTNSEQNSISHLLNYPNPFTTSTAFMFEF
                     DSQNNTIEAEIAIYSVSGKLVKTIREPLSPSGKRFRSDLWDGKDQYGDQLAKGVYLYK
                     VKIYNSFDTAKKVISSDFQKLVILK"
BASE COUNT         2485 a         1976 c         2110 g         2751 t
ORIGIN      
        1 tactgtcaca tgatccaagt cattgggact ttgaagtgat caaggaacat aaggacatcg
       61 atctgacact gtcaggacat acacatggtt tccaattcgg tgtggagatt cccggctggt
      121 tccgatggag tccaagcagg tttgtatata aacaatgggc cggattgtat caaaaaggca
      181 ggcaatatct ctatgtcaac cgtggctttg gttttcttgg ttatgcagga agagttggca
      241 tcctccccga aataaccact ttggtcctca ccggaaagaa ggagtaggcg aaagcgttaa
      301 agtttaccca aaatcctgca atctcaccct ggatttcttt taaatctcat tggattgtgt
      361 atctttagct tggttttatc cttgccgtcc acacatctta aacctatgcc ctatgaaagc
      421 atggattgca tttttgctgt atttccttct tttgggaaat tgccatatac tcaaagcgca
      481 agccatcaac gatgactgct ttagtgccac tgagctgacc aatgtacgta gctggtgttc
      541 atccaatggc tttttcaaca acttttctgc cacaccatca ggatatggcc ctgcgacttg
      601 ttttagcagc tccggacatg atgtatggtt ttcatttacg gctatagcaa cagatattac
      661 cattctggtc aagccgggga gtcttcagaa gccggaaatc gccctgtatg ccgggatttg
      721 tcagggcact ataggcgaat tgaattgtaa gtccaatctt gtgactggag gggcacttga
      781 attgtataag ggaggtttgt ttccgggaac agtctatttg attcgggtcc agggggagga
      841 tgcctcagaa ggtacattcg acctgtgtct ggacaattat tttccgcctg tcaagcccgg
      901 aagtgatatt ataactgcat cagtactttg tgataaaagt tctttcattg tgcagaacgt
      961 tacaggatca ggtagtgtgc ccaatgagtt taaggacgct ccatgcctgg cagatggatt
     1021 taccaattcc gaaaccaatt ctacctggtt taaatggact tgtaaggatg ccggtagttt
     1081 aacatttgtt ctcactcctc tgaatgcgcc ggatgatctg gatttcgctt tgtatgagtt
     1141 aaatggggat ctgaacacaa ccaacagaac actcctcaga tgtgaggctg cttcctgcaa
     1201 gggtcagaca ggtcttaagg aaggagcgac agatatcagt gagccaccta actgtgataa
     1261 aggtcaggac aactggttgg cacccgttca acttgaagcc ggaaagaatt atttgctcgg
     1321 tatcaataac ttcacgattg gaggcaatgg tttcaggatt gaatttggag gaacatccac
     1381 cttcgaaggt ccggttgcgg actttgaaat cctcagtgat acagtatttt gccgggaaga
     1441 acaaattggc ataaaggacc tctcgcataa cagcactgga acaattgaaa agattcagtg
     1501 gatcttcggg caggatgcta caccttccat tgattccatt cagggagaca aaacaatttc
     1561 ttacctgaag cgcggtcaaa agttcatcgt tcagactgtt accagcgaca agggctgcgt
     1621 ggtttctacc atcaagcctg tcgaaatcca gtgctgtggc cctcttcacg agatgaatct
     1681 atacagcgat acaaccataa cattgggaga ttcggttgat ttgcacgctg aagccttttt
     1741 ggaagggaat cagatctttt atttctggga accgcctggc ctgatacaat gtcagggctg
     1801 tcctgctaat aatatcaaga ctttccacga tcaggtgatt gtcgtcaagg caacagacca
     1861 gtataactgt gaggctaaag attctatcca gattagggtg gatgtcaaaa gaccgatttt
     1921 tttccctaat gtattctcac caaactatga tggaatcaac gaccgcttta ccggatatgg
     1981 taactttgta gtcaggacca ttgactatct gcggatattc aatcggtggg gtgctttggt
     2041 ttgggaagga aggaatatcc cggcaaatga tgaaaagtat ggatgggacg gtactttcaa
     2101 ggggcgtgaa gtcaatccgg atgtgtatgt ttatattgcc aaagtgactt ttatcgatga
     2161 tcaatcattt atttacgaag gttctattac cgtggtcagg taggttgctc cgcattgtaa
     2221 gtgttctggc tttattcctt tggccggcaa accttgtatt ctgccaaacc atcaatgatg
     2281 aatgcaagaa agcaatattt attcccaatg ttaaggaata ttgttccggg gacacaacct
     2341 tcagtttttt gggttccacc ccttccaata atggattttc cttatgtcag accgataatg
     2401 gtaatgatgt ttggttttcc ttccatgtta cagcaactga cgttgtcatc aaaattttat
     2461 gtaaggaggc aaatgacttg catgcttcct tatattccgg agattgcggc agcctgaatg
     2521 aagaaacatg tggaaggaga gaggccggac aaaactttct tcagttgtac aaaggaggcc
     2581 tagtgcccgg caccagacta tatcttaggg ttgattcgga aaatggcacg aatttgaaat
     2641 ttggattgtg catcaataac tttttctcac cagctttgga tggtagtgac tgcaaaactg
     2701 ccaatctgat ttgctcaaag gaatccttta cagtaaggaa tgtacagggc ttcggagagg
     2761 tcaatgaact cgggaatgca ccatgtttta gccacgccaa tgagtccaac agcatttggt
     2821 ttagatggat atgcagggat ccgggcacat ttacatttaa acttattccc ttgaaagagg
     2881 cggacgatat tgactttgtc gtgtataaag tccgggggga tgcattcggg tgtgatttgg
     2941 aattggtgcg ttgtatggct accgggatat caccggtcaa ttgcaccaac taccctaatt
     3001 gttgtggctc tacgggcctt cgtgacggag aaaccgatga gtctgagggc gcaggttgtg
     3061 gtccggaccg taataatttc ctgaaaccgc tggacatgga aagcggagag gtatatgcca
     3121 tattaattaa taattacaca tcacaaaatg aaggctttga cttcaatatg ggcggaactg
     3181 caaccctgaa gtcattacaa gccgatatcg acatcataaa gcctgatgca ctctgccggg
     3241 aagagagtct tgaagtgaag gataaaatca tcaatgacct tggtaccatc aaatcacttc
     3301 tttggacatt taccccggat gcatccatca gtacttcaca gggagccgga cctgtatccg
     3361 tccactttga cagatcaggt tataagacaa tctcgcttat tgtggaaaac gatgccggat
     3421 gtaaggcata cgcgcaagat accgtttttg tattttgctg tggaggtaca cttcgggctg
     3481 atattctggg taataccttt gttgattatg gtgaaatggc agaatttggt gttcaatttg
     3541 ttttggaagg cgaagatgtg aagtaccaat ggacacctgc cggtcaggtc aattgtgata
     3601 cctgctccct gataaccctt gatccggtct atgaggattt ccctttatgg gtaaccgtaa
     3661 gtgatgaaaa tggctgtaag gcggaaaacc gggtgttcat taagacaggt aagccggaag
     3721 tttttgctcc aaatattttt tcgcccaatc atgacggtat caatgatcgg tttatgctga
     3781 gcttcgctga aggtatccac agaatccgct cattgaggat ttacaaccga tggggtggat
     3841 tggtgtacga aggtagaaat atggataaga gtgatgccga tggaggatgg gacggcaggt
     3901 ttaaggatcg agacgctgca cccggcacct atgtttatta tgcagagctg gaaacgcgag
     3961 gcggtaaaac aatggtaata aaaggaggct tgacattgat tagataaaca tcagtctatg
     4021 tcaatgaaaa tttacaactt tgcagcatga atatttcctt tcgtactttt ttgggccttt
     4081 gtctgcttgc cttgtctttt caggcatgtg aaaatgacct ggcagaggtg cgcagagtga
     4141 caggggtatc tgtatccgga agttatgaag aaggagtcga tggagaaatt ctttacagtg
     4201 attctgccag actcaaggtg gtcatcaaat ttgatacgct gattcgtatt cttgaccaga
     4261 gcgatccgcg tgatatcttc cctcgtggcc ttattgtttc attttatgat gacaatggtc
     4321 aaatatcaag catattgact gctaacaggg ctgagcggat tgtaagaaaa aatatggtgg
     4381 tggcaacgga ttccgtgcat cttgtcagta gaaatggaga agaactactg accaatgaat
     4441 tgatttggga tgaattgaaa aaggaaattt acaccaaccg gttcgttcgt cttaccaggc
     4501 cgggagaaat tatttacagc ttcgggttca gatctaatca ggattttacg aaatacgaac
     4561 taaatgccct ttccggacgc ttgaaaaaag acaatctgga taaggacttt taatttggat
     4621 tattcccgaa agacttgcct ttgtccgatg cctactttat attaggatca tcatcgacct
     4681 caaatgtgcc aaaatcaaac atcagtgtga agcgcaaggt attgtccaat gcagtcctcg
     4741 tattgccgga gggcacaaga taggacagat tcagcccaaa agtggtgtat ttcaatccca
     4801 gcccaagtgt gaagtatttt tgattacctt tcaatgggct ttcatattga taaccagctc
     4861 tgatagaaaa ctgatcgttg taccaatatt cggcaccaat accaaacata cactctctca
     4921 attcttcctt aaatcctcct gctgcatcgg aaaaagatcc aaatatagcc ggaatcaggg
     4981 ctttttccct gtagtccggt actccattgt tattatcatc ggtaagggca ggatccggac
     5041 ttggcactaa gagtttatta aaatcaacac ccaatgtaag tctgttgtat tgatccagat
     5101 cgacatccca tgccattccg ataccgaggt tagccgggag taagtctctt accggtgaag
     5161 ctttggtata ggatatcctt ccgcccaggt tggtgattgc tacgcctaag gccaggttgc
     5221 tcttggtatt cgttttaagg ggagtccggt aaaatatcga tatgtcggcc gctcccgtgc
     5281 gtgctgtctg aatatccaca ccattgagtt gctgaccggt agcgaggttg gatgagatga
     5341 attttccggt caaagctcca aataatttgt cgcccaatgc cctgctatag gctacagcta
     5401 tctcaaattc attcggtttg ccataaccaa tgatatttcc ttgtgtatcc gtgaacgtaa
     5461 agtctcctaa agaaaaatat ctgatggaag cagctattgc ttcttttttg ctgaatttgt
     5521 aataaccggt taggtacgcc atgaaaatat cattcaatcc aagtcctttt agccatggca
     5581 catagctcat ggacaagccg ccatccttgg ttgatgaagc aagttttgca gcattgaagt
     5641 gcatcgcatt ggcatccggt gatgtggcca gccctaccga acccatacct gaagttcttg
     5701 cgtccggaac gattctgaga aaggatacta cagttgatat tgagttgcga caaggtgatc
     5761 catcaggttc aatccagtgg ttcgaaccta tgggactctc tacacaagta cccgaggact
     5821 gcgcagtcgc cccgcttgtc atgaagagtg taatagcaaa tactaaaaat tggataaatt
     5881 ttttcatatt gaaaaatgta aatatgtatt ttatacaaaa tggccgcaaa tataaacttt
     5941 atattcgtca ttttattgtg tcgtaaggcc cttttctggt ttatttaaga attacgagct
     6001 tctgaaaatc agatgaaatg acttttttag cggtgtcaaa gctgttataa atttttactt
     6061 tatataaata cacacccttt gccaactgat ccccgtattg atctttgcca tcccaaaggt
     6121 cggaccggaa tcgttttcca ctcggactca ggggttcacg tatggtcttg accagtttgc
     6181 ctgaaacgga atatatggca atctcagctt caatagtatt gttctgagaa tcaaattcaa
     6241 acataaaagc agtggatgtt gtaaaaggat tgggataatt cagcagatga gaaatcgagt
     6301 tttgctcact gttcgtgacg ataaattcga tttcagcctc tgccttgtta tttgaaatat
     6361 cccatgctct caatcgaagc gtataatttc cggggtcaag ctgatcaaac tgatatctaa
     6421 cttctccttt gttatattca tccttatctg ccacatagaa gtcgttcata attttttctt
     6481 ttgtcgcatc attgttaagc gtggatacga tttcatgccc tatactgctg ctggaaacat
     6541 tgatgccata atcgtcactc agccgtgcga tgaacaaagg ggttgtattg acaatttctc
     6601 ctgatttgaa atttaaagta ttcatataaa ggtcgataac cggtggctga ttgtcggtga
     6661 ttggattgct gctttcacca ccgattttga cttgatggct gtagcctccc gcatcataga
     6721 ttccattatc ggcataaaat gataatttac cataatcaat ggcgtagttg atatccttag
     6781 gcaggtagaa gctgatgttc cacttacctg cagtgactgt ggccttccct ttgaaaagga
     6841 tattgttctg gaaggaagcc ggataattag ctccgttgcc gtcattgcca cgagagtata
     6901 aggtatatgc cttatcgaat acggtgagtg tgagagtccc gttgaaatct tctattaccc
     6961 tgccatcttc agccctgaca ctccctgcaa gagagacaag ctccatcgcc tttattgtat
     7021 ccggtttagc tgtatctatc ggcgcaccat tgacggtttc caataagatg ttttcccttg
     7081 gaaatgccag tgtcatcgcc ggatctccta aaagtacgaa ggcgagcctg tcagcccctg
     7141 aagaaagatt cttcgctttg gctaatattt ccccattggt taagtaacgt ccattttcct
     7201 tttggtagat tacctgaaat aatttattga taaacacttc attgggcgtg atatataccg
     7261 agcgggtcgt agtcatcagg cctatcattc ccgcatccgg tttggtgatg cacaacttgc
     7321 ccacagaggt gatgtcggga tcgtcgtaag tggcaaatga gcatgtaccg gttatcaaca
     7381 gaggcaacct gttgctgttt ttcatcccat tgagagtaag cctgtcgatg attctttcct
     7441 gagcaagacc atcaacacca ccatgaccga catagttggc tatcaggacg cctttgttgt
     7501 attggtcatt catctggctg acggctgttg gatagctctg tcctcccaga ttagattcct
     7561 gcttgaaaat atccagataa atcttcgaaa aattgatacc gggattgctg ctttggatct
     7621 tattgaatat tttttcggat tgtactatga aatcatcact gtttacatta tcccagtcgt
     7681 ctgatatcaa catcagataa ttgcgccaat ctccgaagga atccttgttc ttctcataac
     7741 taatgatttt gttgatgatg gactgagcct gacttttatc atagacagga aaccggccga
     7801 cataaacgtc aatcgttcct ttttcaattt caccgtcttc ttcatccagc atgccgaaat
     7861 aatcatcgga tggaatagat cggatgggtt cgaggattgc cctggggaat tcatatacag
     7921 gtacataatt ggaattctcc ttcttggagg tggcattgaa ggtcagcccc cggtaatcat
     7981 agctcccatc acccaacaaa agaatatatc tgaatttttc cggattgcgg tagtaaaaca
     8041 tacgtgccat atcccggata ccggttgggt cctggccacc acctgaaaac tcattcctga
     8101 tatggtccac atcgacaata gcaacatcaa taccggatat tgagcttctg tgattggcgt
     8161 aatttcttgc ttcttccgca aatttggcat gatatatgat gaccatgtcc ggggtggcca
     8221 ttccatgaat atcttgcgga gttacaaggc ctatcttctc gggtgtcttg atgtcgtttg
     8281 ttccaaaaca gataaactcc cgttgttggg ggtagggtgc cactatgctg ccacttcccc
     8341 ctgtggggct gatcttacac tgtgtaacat taaacaagtc tgagatgtcc caaactgcat
     8401 cggtagaact taagcctgtc agattgtatc tgatgttgcc ggtgatcccc gggtttgaaa
     8461 gcctgaaagc cattgctgat tggggctttg aaagatatct tttgatgttg acctcaacat
     8521 agtccatcca tccaaggctt tctgtagcca cttgagggaa gtcgaaggag atcttcagtg
     8581 gaccgcctgc ttgagtaaat gcgctttttt tcgaattgta tgaagctacc cggctttctg
     8641 aatcctcaat cctcgtggtc tcgaattgaa tttctgcagc ctgattatcg gcaatcatgc
     8701 tgatgactga ccgcgacttg cttctgacgg caaaagccat cttgacatag cctgtttctc
     8761 ccgcaaccag attatccacc ttcagcaagc ctgacaggtc tttggttcga ttactcccgg
     8821 ttatcaattc gtcaagccat agctgccctc caccaatttt agcatattcc cgaagtaaat
     8881 tgaccttatc aacctcgtaa tgttcaatat ggtcatagat atctgattcc acatcaaaag
     8941 cctgcccccc atccttcagg acaattcgct tacccggagc accttctacc tggatgaaaa
     9001 aatatttgta atcttcataa atattggtca cccgcgtgaa agtactgtca ctttccttat
     9061 tccacctgtg aggtccggga gaataaaaaa gaatgtagtc tgatgcatcc cacttgccat
     9121 catctccgcc tatgacctga atagccactt ctgccagatc gtctgtcctt gggaggttgt
     9181 ttttttcagg taaaggacct gggttggtag taaatatctt tattttggat ggatcgattc
     9241 ccgatgtatt gatacccaga tctgccataa attggttgtc aatccgataa attccggcat
     9301 cggctgatct gaacttgtag gt
//
LOCUS       DAOQQN010000076         9299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2779_length_9299_cov_5.883059, whole genome shotgun sequence.
ACCESSION   DAOQQN010000076 DAOQQN010000000
VERSION     DAOQQN010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9299
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2779_length_9299_cov_5.883059"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1732)
                     /locus_tag="PLG24_04310"
     CDS             complement(<1..1732)
                     /locus_tag="PLG24_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24142.1"
                     /translation="MNKNLFFLVLLFVVGMANEMSALMPPGHNKPTEKQIELRELCSP
                     ATASVELNINNVRALLLNGGDVWWNRDDPRYIVPNVTVKPVSSIFAAAVWIVGYNGGS
                     IKGAGRMYGGNSNKHDWFPGPLNDLTGQITVQECSNWDRFFEVRGEEIDKHIAQFKSF
                     ELKGEDYPESLIPLNVRGWPSRGNPYFKSIYNWSLPTTTAALAPFYDYDGDGEYDPAK
                     GDFPIIEVRGVAGACESTPENPVPPTYADQMFFWIYNDNGNTHSEFNGTPINMEVQVQ
                     GYGFKTNDQLNDMTFMRYKLVNRSPTVTDKCLFGWWVDSDLGCPDDDLIGCDSTRSLV
                     YYYNIDGTDGNSGPDQTLCNAGGGSVNTYGRKVPILGIDYFEGPTIDTIINGKEVNFD
                     IGMTSFTYWNRESAEPGTVDPNNLQQLYYYMDAKWKDGTPFTYGGSGYKSGGAVTRYP
                     FSDAPTDINGWSMKTVTNLPGVDRRTAQSTGPITLQSNKTNYLVVGVPWVPDQDYTGP
                     NNAPGLNDLYAADDICQGLFDNCFKIFDGPDAPDVDVIELDRKIVLALSNDPEKSNNF
                     NLNYHGFNPGI"
     gene            complement(1798..5409)
                     /locus_tag="PLG24_04315"
     CDS             complement(1798..5409)
                     /locus_tag="PLG24_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ24143.1"
                     /translation="MSRILLLFAFLFSISVLVEAQTSLGGKIVDNESGEVIIGATVLL
                     KRSGVQTDGTITDEYGNFYFSSVAPGKYDVHITYTGYAPVIVTNVDVFNGKANKLPVT
                     KLDTETKLLQDVVIKAYKNPLIEQDNTTQGTVMTQEQIAKAPVKNIQALAAASGGVSS
                     ANGSAISIRGSRTSSTNYYIDGIRVAGGYLPPNEIEQLQVITGGLEAQYGDVTGGLIV
                     ASTKGPSSSYSVYVDGETSKFLDPYERTEINVSTSGPILKNKNNQSVIGFRIGARYLG
                     STDGSPSFDPIRYMSKDRIAQLEANPVRNIGAGGIYPEGEFNLTENTFDSKIGINEKN
                     RNIDLNGKLDFRLSNTIDAQIGFGYSDIEGKFSPSRAWQFANYENNPTSYSNRYRGNI
                     RIRHKLSTTNKGLLQNVGYVVIGGYEKLTSRNEDPRHGNKFFDYGYVGRFTSKLGEPI
                     IRLEDDEFKQVYAIIEQYDGTFIPGTQNPVLANYNNFLTPEQIINQEFITYNGQQAQS
                     NLYSTIYGLYSNVGQVYDTLSKSESDTYTGRLDINFDIVPGSSETGRHSINLGFLYEQ
                     NISRSYSLSPRNLWTLGNNLANTHLKELDYNATPIDTVDFFGNEVPIYPYKVTDQLGN
                     RFYRAVREKLGVPLSDPIFINNLSPDQLSLDMFAPGELTSYGVLGFYGYDYLGNKINN
                     ASFNDFFTKTTGSENGVRAMLLAPFKPIYQSFYIQDKFQARDVIFRLGLRVERYDANT
                     KVMKDQYSIYEISTAQEYYDQIGQQRPDNIDPDAFVYLNTAGNDASGVKAFRVGDTWY
                     TPQGVATLNPDDIFQKGITNPRYKLLPGEVNKPDIQLATYDPNRSFKDYKPQVSFSPR
                     IAISFPISEDANFFGHYDILVERPSNNYASGLSYLYWENQGSFNNPDLKPSKNIDYEI
                     GFQQKLNNSSALKLSLYYKEIRDLIQSTLILYTNNISGKMLTYRNIDFSTVKGIQIQY
                     DLRRTSNLTLQANYTLQFADGTGSDPGTQAAIAKNGQVRVLSPLDFDERHALKFTADY
                     RFDEGKSYNGPEWFGVDVFSNAGVNIITSAVSGRPYTKAATPTQFGGSGTIGTYNGQR
                     YPWNFNIDLRVDKTFRLGSVDAKRSPSINIYARVTNLLNTRNIYGLYKASGSPFDDGF
                     LPTFLGQNLYSAIDLNQVATGLGRTSAEYINAYNWLMYNPGFFNGPRRIYIGASFNF"
     gene            complement(5580..6524)
                     /locus_tag="PLG24_04320"
     CDS             complement(5580..6524)
                     /locus_tag="PLG24_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ24144.1"
                     /translation="MKNVLITDDCHPDLVEGLNKAGMIVDFMPGLTLEEIDDCLYKYF
                     GIIINTRVKMDRTRIDNGPGLRFIGRMGSGLDIIDLKYAAEKGVAVISSPEANATAVG
                     EHSLSMLLSLLNKLPLGAQQVHNMSWDREAARGRQLAGSTVGIIGFGHTGPAFAEALS
                     GMRLVIKVFDKYKDVPYQKSGNSEIIPSGMEEILESCDFISFNLPLTSETAFLIDETL
                     ISKMKKGVILINAARGKLMNLDAVINGLKSGRIGGCCLDVFPNEKPATFSEQEVEQMQ
                     FLTAHPNVILTPHVAGWTFESKAKIGEVLLQKIIKNFG"
     gene            6631..7320
                     /locus_tag="PLG24_04325"
     CDS             6631..7320
                     /locus_tag="PLG24_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24145.1"
                     /translation="MFKLNKLHLFILLVTIAAIGCKKNESDDNFPRNVDIYMRYLLEG
                     NQFNGLAKVSYADTIAIGEPDTATYFFTINGKDMKQVISPGGIKNYSVKANMPFSDQY
                     EVKIKGPDHSITTKSTKIIPIDSFQLPRAISKSTALNIEYFGSPLNANESIIAMLVAS
                     DGKTASATLNGPASSKSINIKADKLSELISGLADIYLIRTYSDEDVTGKLHFFIKNEF
                     YSRTKQVELTD"
     gene            complement(7652..8722)
                     /locus_tag="PLG24_04330"
     CDS             complement(7652..8722)
                     /locus_tag="PLG24_04330"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HMZ24146.1"
                     /translation="MKKLIFTFLFFSTTYLIIAQNDARVNEIRKSIAAKDTFGWVKGA
                     GLGFDFGQLLQINPAVGAGENRIGLGAVGSFFANYKNGKFAWDNNVDLLFGIQKLGSG
                     YSTLKPGVRIPFQKSIDEFRFNSKIGYKTSQTSKVYYSLYTTFLSQLTPTFIDTSVVD
                     PVKGSYLKDLSGKDQGPIAKFFSPARISISPGIDYKPNSKLSLFLSPASAKMIIVADK
                     NIAAIPARNAAGKPIGQVNIHGTEWKSPTDYKQFLMQLGATLNAQYVDKFLNNKLNFK
                     TGLTLFSNYLNNPQNVDVDWSTETAFMIWKGIAITLNTRLFYDDDIPVQITDNNVAGG
                     LNGLGKRVSFTEQLYIKYNYIF"
     gene            complement(8746..>9299)
                     /locus_tag="PLG24_04335"
     CDS             complement(8746..>9299)
                     /locus_tag="PLG24_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HMZ24147.1"
                     /translation="VVAVTDMLNLTTVSTYLTSLINYLPQAFTAFAFFIAGLLFSDFL
                     RKVVYTAAKSIGIPSAKVISTILFYFLFINVFISALTQAGIDTEFIQSNLTMIIGGIV
                     FAFGLGYGIASKDIMASVLAGYYNKRLHEGDRITIGSVTGTITSISKTDLIVTNESDS
                     VIIPLSEISKGVVIVHQTKDNKN"
BASE COUNT         2913 a         1952 c         1642 g         2792 t
ORIGIN      
        1 aaattccagg attgaaacca tggtaattaa gattaaagtt gtttgatttt tccgggtcgt
       61 tgctcagagc caatacaatt ttcctgtcca attcgatgac atcaacatcc ggtgcgtcag
      121 gaccatcaaa tattttgaag cagttatcaa aaagtccctg gcaaatatca tcagcggcat
      181 ataaatcatt caaaccagga gcattatttg gaccggtata atcctgatca ggcacccatg
      241 gcacaccgac cacaagataa tttgttttat tggattgtaa agtgataggc cctgtagatt
      301 gggcagtacg acggtcaaca ccgggtaaat ttgtcactgt cttcatagac cacccattga
      361 tatcagttgg agcatccgaa aaaggatatc gtgtgacagc ccccccggac ttataacctg
      421 aaccaccata agtaaaagga gtaccatcct tccacttagc atccatataa taatacaact
      481 gttgaaggtt atttgggtca acagttccgg gttccgcact ctctctgttc cagtaagtaa
      541 atgaagtcat tcctatatca aagttaacct ccttgccatt gattatcgta tcgatggttg
      601 gtccttcaaa atagtcaatt ccaaggatag gcacttttcg cccgtatgta ttcactgaac
      661 cgccgccagc attacaaagc gtctgatcag gtcctgagtt tccgtcagta ccgtcaatat
      721 tatagtaata tacaaggctt cgtgttgagt cacaccctat caggtcatca tcaggacatc
      781 ccagatcgga gtccacccac catccaaata aacacttgtc tgttaccgta ggtgatctgt
      841 tgaccaattt ataacgcatg aatgtcatat cattcaactg gtcgttggtc ttgaaaccat
      901 atccttgaac ctgaacttcc atattgatag gcgtaccatt aaattcagag tgggtattac
      961 cattgtcgtt ataaatccag aaaaacatct ggtcggcata ggttggaggc accggattct
     1021 cgggagtaga ttcacaagca cctgcgacac ctcgtacttc gatgatggga aaatcacctt
     1081 tagcgggatc gtattctcca tcgccatcgt aatcataaaa tggagccaat gcagctgttg
     1141 tagtcggaag actccaatta tatattgatt tgaaatatgg atttcctctt gaaggccagc
     1201 ctcttacatt cagaggaata agactttcag gataatcttc acctttcagt tcaaaagatt
     1261 taaattgtgc gatatgcttg tcgatttctt caccgcgcac ttcaaagaat ctatcccaat
     1321 tggaacattc ctgcactgta atctgaccgg tcaggtcatt caatggtcca ggaaaccagt
     1381 catgtttgtt actattaccg ccatacatcc taccggcgcc cttaatagat ccaccattat
     1441 aacctacgat ccaaactgca gcagcaaaga tggacgatac cggcttgact gttacattag
     1501 gcacgatata acgaggatcg tccctattcc accaaacatc accaccattc aacagcagag
     1561 cccgaacatt attgatattc agttcaacgc tggctgtcgc aggagaacaa agttcccgca
     1621 attcgatttg cttttccgta ggtttattat gtccaggggg catcagagca gacatctcat
     1681 tcgccatgcc tactacaaaa agcaatacca ggaaaaataa atttttgttc atttgaattc
     1741 tttttaaata tttatcttca aatatctaag gttaataaat atcagtttaa caatgaatta
     1801 aaaattaaaa ctagcgccaa tgtatattct cctagggccg ttaaaaaatc caggattata
     1861 catcagccag ttatatgcat tgatatactc agctgacgtc cttccaagac cggtagcaac
     1921 ctgattcagg tcaatggctg aatataggtt ctgaccaagg aaagtcggca agaatccgtc
     1981 atcaaatggt gagccggaag ctttataaag accatatata ttcctggtgt tcaacaaatt
     2041 agtcaccctc gcataaatat tgatagacgg agatctcttt gcatccaccg aaccaagtct
     2101 gaatgtctta tccactcgta aatcaatatt gaaattccaa ggatatcttt gtccattata
     2161 agtaccaata gtaccacttc ccccgaattg agtcggtgta gctgccttgg tataaggtcg
     2221 gccggaaacc gcagaagtaa taatattcac accagcattg gagaaaacat cgacgccaaa
     2281 ccattcaggg ccattatatg atttaccttc atcaaaacga tagtcggcag taaatttgag
     2341 agcgtggcgc tcgtcaaaat caagtgggct aagcactctt acctgaccat ttttagcaat
     2401 ggctgcctgt gtacccggat ctgatcctgt accatcagca aactgcaaag tatagttggc
     2461 ctgcagagtc agattgctgg ttcttctcaa gtcatattgt atttgaatac cttttacagt
     2521 actaaagtca atatttctgt aagtaagcat tttaccagaa atattattag tgtaaagaat
     2581 caaggtactt tgaatcaaat ctctgatttc tttgtaataa agagaaagtt taagggcgga
     2641 agaattattc aatttttgct ggaagcctat ttcataatct atattcttgg atggcttcaa
     2701 atcagggtta ttgaaagacc cttggttttc ccaatataaa taactcaatc ctgatgcata
     2761 gttgtttgat ggtctttcta ccaggatatc ataatgacca aagaagttgg catcctcaga
     2821 gatagggaat gaaattgcaa tcctaggtga aaaacttacc tgaggcttat aatctttaaa
     2881 tgagcgattg ggatcatagg tagccaactg aatatcaggt ttatttacct cgccgggaag
     2941 gagtttatat ctaggattgg taataccttt ctggaagata tcatccggat tcaatgtagc
     3001 cacaccctgc ggtgtatacc aggtatcccc tactctgaaa gctttaacac ctgaagcgtc
     3061 gttacctgca gtgttcaggt aaacaaaagc atcgggatca atgttatccg gtctttgctg
     3121 gcctatctga tcataatatt cctgagcagt tgaaatctcg tagatactat actggtcctt
     3181 cataaccttg gtattcgcat catatctttc cacacgtagt cctaacctaa agattacgtc
     3241 ccttgcctga aacttatcct gtatataaaa agactggtag attggtttga aaggagctaa
     3301 aagcattgct ctcacaccat tctcggagcc tgtcgtcttt gtaaagaaat cattgaaact
     3361 tgcattattt attttatttc ctaaataatc atagccatag aagcccaaaa caccatatga
     3421 ggtgagctct ccgggtgcaa acatatcaag cgatagttgg tctggagaca aattgtttat
     3481 aaaaatagga tcactcaatg gaacccccag tttttcgcgt acagccctgt aaaatctatt
     3541 acccaactga tcggtaacct tgtaaggata aattggaact tcatttccaa aaaagtcaac
     3601 cgtatcaatt ggagtcgcat tatagtccaa ttctttcagg tgagtattcg ccaggttatt
     3661 acccaatgtc cacaaattcc ttggtgaaag agaataagat ctggaaatat tttgttcata
     3721 aaggaaacct agattgattg aatgtcttcc tgtctctgag gaacccggaa caatatcaaa
     3781 gttgatatct aatcttccag tgtaggtatc actttctgat ttgctgagag tgtcgtatac
     3841 ctgaccaaca ttactataca agccataaat tgtactatat aagttggatt gtgcttgctg
     3901 tccattataa gtaataaact cctggttaat tatctgttca ggtgtcagga aattgttata
     3961 gttagcaagc accggattct gagtacccgg aataaaggtg ccatcatatt gttcgataat
     4021 ggcatacacc tgtttgaatt cgtcatcttc caacctaata atcggttctc ccagtttgga
     4081 tgtaaacctg cctacgtatc catagtcaaa gaacttgttc ccatgacgag gatcttcatt
     4141 tctggaagta agcttttcat agcctccgat aactacatac cctacattct gaagcaggcc
     4201 tttgttggtt gtcgaaagtt tatgacggat tctgatattt cctctatatc tgttggagta
     4261 tgaagtcgga ttgttttcat aattagcaaa ttgccatgca cgtgagggtg agaactttcc
     4321 ttctatatca gaataaccaa aaccgatttg tgcatcaatt gtgttagaaa gccggaaatc
     4381 caattttcca ttcaaatcga tatttctgtt tttctcgtta attccaatct tagagtcaaa
     4441 agtattctca gtaagattga attctccttc cggataaata cctccggctc cgatatttct
     4501 aacaggattt gcttccaact gagcaatccg atccttactc atatatctga ttgggtcaaa
     4561 tgaaggactt ccatcagtcg aacccaaata acgtgcacca attctaaacc caatgactga
     4621 ttgattattc ttatttttaa ggataggacc tgaagtagaa acgtttattt cagttctttc
     4681 ataagggtct aaaaatttag aagtttctcc atcgacatat acactataac tgctacttgg
     4741 cccttttgta gatgcaacga tcaaaccacc ggtaacatcg ccatactgag cttccagacc
     4801 accggtaatg acctgcaatt gttcaatttc attcggaggg agatatccac ctgccaccct
     4861 gattccatca atataataat tagtagaact tgtacggcta cctctgatgg aaattgcact
     4921 tccattggca cttgaaacgc caccggaagc ggctgctaag gcctgaatat tcttcacggg
     4981 ggccttggca atctgttcct gagtcatcac agtcccctgg gtggtattat cctgttctat
     5041 caaaggattc ttataagcct taataacgac atcctgtaac aacttggtct ctgtatccaa
     5101 ttttgtcacg ggaagcttat tggctttacc attgaaaaca tccacattgg tcacaatgac
     5161 aggtgcatat ccggtataag taatatgtac atcgtactta ccgggtgcaa ctgaactaaa
     5221 atagaaatta ccatactcat ctgttatggt accatcagtc tgaacaccgc ttcgtttcaa
     5281 taaaaccgta gccccaataa taacttcgcc tgactcatta tcaacgatct tacctccgag
     5341 gcttgtctga gcttcgacta atacggaaat actaaacaaa aaagcaaaaa gtaaaagaat
     5401 tctgctcata tcaagtcttt tatcaaatta agttttacaa gctgcaatag taacaaaatt
     5461 ttaagatata aaaccaatta ttcatcagac tttatttgtc ataaatctaa ataatgaaac
     5521 ttcgttttaa acaagctaca tctatttatt tgataatcaa atacaaacta attatgaatt
     5581 tagccaaaat tttttataat tttttgtaaa agaacctctc caatcttagc tttggactca
     5641 aaggtccatc ctgctacatg aggtgtcaga atgacatttg gatgtgctgt aagaaactgc
     5701 atctgctcca cttcctgctc tgaaaaggtg gctggtttct cattaggaaa aacatcaaga
     5761 cagcatcccc caatccttcc tgatttaagg ccattgatga cggcatcaag attcattaac
     5821 ttacctcttg cggcattgat cagaattaca ccttttttca tcttacttat caacgtttca
     5881 tctattaaaa acgctgtctc agaggtaaga ggaagattaa agcttataaa atcacaactt
     5941 tccagtattt cttccatgcc agatgggata atttctgaat tacctgattt ctgatacggt
     6001 acatctttgt atttatcgaa taccttaatt acaaggcgca ttcctgaaag agcttcagcg
     6061 aatgcaggcc cggtatgtcc aaaaccaatt ataccaacag tactccctgc caactgtcgc
     6121 cctcttgctg cttcgcgatc ccagctcata ttatgcacct gttgagctcc taatggaagt
     6181 ttattcagca gtgacaagag catagaaaga gagtgctccc ctacagccgt ggcgttggct
     6241 tcaggagaac ttataacagc aacgcctttc tcagcagcat acttaaggtc aataatatca
     6301 agaccggagc ccatcctccc tataaatctc aaccctggac cattatctat tctagtccta
     6361 tccatcttaa ctcttgtgtt tataataatt ccaaaatact tgtacaggca atcatcaatt
     6421 tcttcaagag tcaggcctgg cataaaatca acaatcattc ctgccttgtt taacccttcc
     6481 acaagatcag ggtgacagtc atccgttatc aaaacatttt tcataaaagc aaatgtaatt
     6541 caaatgatac cataaaaata gtcttaatgg ttagctttgc caaaaattag accgagaata
     6601 aaaaattacc gaataatcat aaaatactcc atgttcaaac ttaacaagtt acatttattc
     6661 atactattag tcaccatcgc tgctattgga tgtaagaaaa atgaatcaga cgacaacttc
     6721 ccccgcaatg tagatattta tatgcgttat ctgttggaag gtaaccagtt taatggcctg
     6781 gcaaaggtca gttatgcaga taccattgct ataggagaac ccgataccgc cacctatttt
     6841 tttactataa atggtaagga tatgaagcaa gtgatatctc cgggaggtat taaaaactat
     6901 tctgtaaaag caaatatgcc tttttctgat cagtatgaag taaaaattaa gggacctgat
     6961 cattcaatta caacaaaatc taccaaaatc attcccatag acagttttca attacctaga
     7021 gctatttcaa aatcaactgc attgaatatt gaatattttg gtagtcctct caatgccaat
     7081 gaatcgatta ttgcaatgct tgttgcctcc gatgggaaaa ctgcctcagc aacacttaat
     7141 ggtccagctt ccagtaaatc catcaatatc aaagctgata agttatccga attaatctca
     7201 ggcttagcgg atatctacct tattcgtact tacagtgatg aagatgtgac aggcaaattg
     7261 cattttttta ttaaaaacga gttttatagc cggacaaagc aagttgaatt aacggactga
     7321 tctcttagta ttatgaaacc taatccaaga aatccctcca tttaggttat ggaatgaatg
     7381 gaggagtaaa aagttaaaac ggatattacc ataacctact ggtctttcat agaataagtc
     7441 caaggtgtat gaaattgcaa caatagttga aaaccaatat aggtatttac ccaccaatat
     7501 ttatatccaa gaaagaatat cgaaatggaa tacacattga atcctgaaat catttttatt
     7561 caaataaaaa atcgggtgat gatagccatc cgcccgattt aaatcgctta ttatattgct
     7621 taaatataat tgtcttacaa ataaatatac tttaaaaaat ataattatac ttaatataca
     7681 attgctctgt aaaactcact ctttttccta atccgttgag cccgccggca acattattat
     7741 cggttatctg aacagggata tcatcatcat agaacaacct cgtattcaaa gtaatagcaa
     7801 tgcccttcca gatcataaaa gccgtctccg tagaccagtc aacatcaaca ttctgtggat
     7861 tattcaaata atttgagaaa agggtaaggc ctgtcttaaa gttcaactta ttattcagga
     7921 atttgtcaac atattgcgca ttgagtgtag ctccaagctg catcaggaac tgtttgtagt
     7981 ctgtcgggct cttccattcc gttccgtgaa tattgacttg accaattggt tttcctgctg
     8041 cgtttcgtgc agggattgca gcaatattct tatcagcaac tataatcatc tttgctgatg
     8101 ccggagaaag gaagagagaa agtttgctat taggtttata atctatacca ggagaaatgg
     8161 atattcgggc aggagagaaa aacttggcta tcggaccttg atcttttccg gataaatcct
     8221 ttaagtaact acctttgacc ggatcaacca ccgatgtatc aataaatgtc ggggtaagtt
     8281 ggctaaggaa cgttgtatac aatgaataat ataccttact agtttgggaa gttttataac
     8341 caatctttga attgaatctg aattcatcta tactcttttg aaatgggatc ctaactcccg
     8401 gtttcaaagt tgaataacca ctgcccaact tttgaatacc aaataacagg tccacattat
     8461 tatcccatgc aaattttcca tttttataat tggcaaaaaa gctaccaact gcgcccaaac
     8521 ctatccgatt ctcacctgct cccactgcag ggttgatttg caataactgc ccaaaatcga
     8581 atccaagacc ggctcctttt acccagccaa aagtatcctt agcggcaata gattttctaa
     8641 tttcattaac ccgagcatca ttctgagcta ttattaaata agtcgtagag aaaaataaaa
     8701 aagtaaatat tagttttttc attcttttat tcatttgaat aataatcaat tcttgttatc
     8761 tttagtttga tgtacaatga ctactccttt tgatatctcg ctcaagggaa taatcacgga
     8821 atcactttcg ttagtaacaa tcaaatcagt tttggatatg gaagtaattg ttcctgttac
     8881 ggaaccaatg gtaatcctat ctccttcgtg tagtcgctta ttgtaatagc cggccaaaac
     8941 acttgccata atatctttgc tggctattcc ataaccaagt ccaaaggcaa aaactatacc
     9001 tccaatgatc attgtaagat tggattgaat gaattcagta tcaattcctg cctgagtcaa
     9061 tgcactgatg aaaacattga tgaaaaggaa atagaacaaa atggtggata taaccttagc
     9121 tgaaggaata ccgatggatt tggctgcggt atagacaacc ttgcgaagaa aatcagaaaa
     9181 caaaagaccg gcaataaaga aggcgaaggc ggtgaacgct tgtggcaggt aattaatgag
     9241 gctagtaaga taggtactaa ctgtcgtcag attgagcata tctgtcactg caactacaa
//
LOCUS       DAOQQN010000077         9223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2817_length_9223_cov_5.436082, whole genome shotgun sequence.
ACCESSION   DAOQQN010000077 DAOQQN010000000
VERSION     DAOQQN010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9223
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2817_length_9223_cov_5.436082"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLG24_04340"
     CDS             <1..323
                     /locus_tag="PLG24_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HMZ24148.1"
                     /translation="FGMVNGLLPCGLIYLAVAAAITTGNIGGSALVMASFGLGTMPLF
                     SLFLFGFNRINLSFRQKVNSFMPFLVLLTAILLILRGMNLGIPYVSPYLNVEGTTPKV
                     IHCD"
     gene            complement(551..6103)
                     /locus_tag="PLG24_04345"
     CDS             complement(551..6103)
                     /locus_tag="PLG24_04345"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HMZ24149.1"
                     /translation="MAPKVFLYPLIILAFSTSFLACKKNTESNEKNPFIYANTSGVIS
                     RKQPVKVVFADNMVDSKSVGGFVASGAVSVSPSVKGNAVWQDEKTIVFTPMDEFVSDK
                     QYDFSINIGALQDNVPKEFRHFNFNIRTIRQDAAITESGWDTPSQSDYTEQTYKGAIL
                     TADEVEASKIEALLTASIKGQSLSVAWNHDSKGVTHNFTIRGIKRNSSDQKMEIRFDG
                     KPIGVNNVITREVPVAPLTEFKVHDIYFSQDEDFFFIINFTDPVSATQNLGGMITIDG
                     FDEVVNYSVQNNNILVYPSKRITGEKTVRVAPSVLNSRNESLGKPWSKVVNFLDTKPA
                     VRFAGKGNIMPESDGLILPFEAINLNAVDVEIVKIFSDNVLQYYQNYNGYEDSYEINR
                     VGRIILQQKIQLSSLNSAINKSSWVRYGLDLNKLITTDRNSIYQVRIGFRRAYTSYLC
                     KSGGDWKERELSTEPVAYGEEPVSIYDHNYDGFAEEYDEDYNWLNRENPCKPMYYNKD
                     NFIAQSVYKSNIGLIVKGNDNGNFFVAATNLLTAKPLSGVEIKFYDFQQQVIDKGLSN
                     GEGFLNITLKSRPFMAIAEKDGDKSYLLLDDGRSLSLSKFDVGGDSYQKGLKGYLYAE
                     RGIWRPGDSIFLNCIIEDKLKTLPASHPIQLEWRDPRNTIIARKTYNYTSGSILPIHL
                     ATSPDAPTGVWAATVKVGGATFTKLMSIETVKPNRLKIKFDVDKKQLMAGQPLVNASL
                     NAAWLIGTVAGNLRAVVDANIRVQSTPFTKYSDFIFSNSSSGKTFEKKVFEGNTDASG
                     NASLKMNINLNEEIHQISNIVFKTTVFEQGGNFSVDYTSETYSPFKVYVGVSVPHNQW
                     GEPSIPDKKNAPISAIVVDQNGNPLSGINATINVYKVDWRWWWESDYSDANYYTGDFN
                     EVPLAHYTGKTKGDGKIMFDFRPPAWGRYLIKVTSDASPHVAGTFIYSGYPDYADAEQ
                     MSSIATSLPLGIDSKEVKVGETINLSFTAAAGSKALVTIEGGSSIVEHKWFDCKEGVN
                     EVSIKTTDAMGSNAYAFVTLMQPHKHPENDMPLRMYGVLPFKIVNPALVLNPVISMPQ
                     EIQPDKKAEISVSEQNGNEMYYTIAVVDEGLLDITKFKTPNPYNFFYAKSALAVRTWD
                     LFDYVLGAYSGKLNGIFAVGGDMAAAQIEGAPKANRFVPAVVNLGPFKLKKGEKARHH
                     FTIKNYIGSMRAMVVACNDKAFGAAQQSVKVVKPLMVTTTLPRVLSPGETFNLPVNVF
                     VTKNNIRNVTVKINDKNGYMSVTGSSSKSLTFESSGEKMVYFPVTVSKQEGVSSIDVT
                     ATSGNESSTETVEIQVRNPNLPIVTSKEYVLNAGQSVQLQNLLSGMESSVTSSLEFST
                     FPSIDLSRNLTELIDYPYGCLEQTISKAFPQLFLKNLTVLSKADQDRIKSMVEVAMNK
                     LPTFQKPEGWFAFWPGSNFDDPYTSIYAGQFMLEAKKAGFNVPQGSLETLISSQRRAA
                     RLWQPKQLKAGLYPHNADIDQAYRLYMLALAGSPEIGAMNQLKELNTISVPARYYLSA
                     AYSLSGKKDVSAALIEKAELSTKPYREFGYSFGSDLRDEAIMLNALTLAERKEDAASV
                     AKSIAKKLSGGYWYATYSKSYGIWAVATYLAKYPPAGSVKASYTVNGKTVEVNESSAA
                     HMVEFGSQVKMAGSKVVNKSKGVLYVTMVQKGKPALTDQTAIDYSLALSIKYTDKSGN
                     RIDPVKLKKGTEFYAIATVKNTAPYEFQIQNLALTQIFPSGWEIVNDRMQAGMATSES
                     SYLDYQDIRDDRVNTFFSLYQGAEKSFKIKLIAAYAGKSYVPAASCSAMYDNTISARV
                     PGKWVTIE"
     gene            6538..6699
                     /locus_tag="PLG24_04350"
     CDS             6538..6699
                     /locus_tag="PLG24_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24150.1"
                     /translation="MSQEENKTNTKTQWMIFTVSTIALILLLVYLPSFFWVALPFQLT
                     SFAYAMDWV"
     gene            7183..7479
                     /locus_tag="PLG24_04355"
     CDS             7183..7479
                     /locus_tag="PLG24_04355"
                     /inference="COORDINATES: protein motif:HMM:NF006617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HMZ24151.1"
                     /translation="MSFKFAKNEYNEMEDLILELKEKLIAQLNLKHLTPEEIDSDQPL
                     FGVRIGLDSIDALEIIVMLDNDYGIKLTNQEEAREIFTSVRTIANYIHKHQANA"
     gene            7472..8662
                     /locus_tag="PLG24_04360"
     CDS             7472..8662
                     /locus_tag="PLG24_04360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HMZ24152.1"
                     /translation="MPESRVVVTGMGIMAPLGRGVSANHAALVDNRHGLGDITILNTI
                     HRGSLPAGEIKLTNTDLASIANFQGENIPRSVLLSAVAIQEALASAGLHHNHDINFYY
                     STTVGGMDISEELNDKRLKGQDIDFSGFKYHDCGASTMLLGDFFGFTGRRYSLSTACS
                     SSANCIGRAMMDIKTGRARYAVAGGADALSRFTLNGFNSLMILDKELCNPMDQNRRGL
                     NLGEAAAFLVLESEENAVNRGAEILAIVSGFGNTNDSHHQTASSESGIGAVLAIREAL
                     NDAGLKAENISYINAHGTGTQNNDLSEGNALETVFGKVPPTASTKSYTGHTLAASGAV
                     EAIYSVLAIKNQEMYPTLRFKEAIVGHGFKSFDFTIQGEVNHVLSNAFGFGGNCSALI
                     FSKY"
     gene            8666..>9223
                     /locus_tag="PLG24_04365"
     CDS             8666..>9223
                     /locus_tag="PLG24_04365"
                     /inference="COORDINATES: protein motif:HMM:NF025100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HMZ24153.1"
                     /translation="MKAYINHINAISRYQYLEGSIIEQSEIIEPDYESILDVRAMRRL
                     SKILKMGLFTALSVKNQANRQPDGIICGTALGCLADTYQFLKNMSDRDEQLLTPTAFI
                     QSTHNTLSGTLGIMLQNHSYNITWSNREFSFYDALEDAIIRVNKCTDDHILLTAADEM
                     PRQVAEIIDSIPLDCPVPINSEGAAS"
BASE COUNT         2713 a         2026 c         1745 g         2739 t
ORIGIN      
        1 tttttggcat ggtaaacggc cttttacctt gcgggctaat ctacctggcg gttgctgctg
       61 ccattaccac cggcaatatc ggaggtagtg ccctagtgat ggcatcattt ggcttgggga
      121 caatgccact atttagcctt tttttattcg ggtttaacag aatcaatctc tcattcagac
      181 aaaaagtaaa ttcctttatg cctttcctgg tgttactcac agcaatcctt ttgatactcc
      241 gtggaatgaa tctaggaatc ccttatgtaa gcccatatct caatgtggaa gggacaacac
      301 ccaaggttat acattgtgat tgaatgtccg caaatattat ttgctccaaa tgcatagtca
      361 gtataatcac ccaataatga accagattga cattcactcc ggataaccat cagtcataaa
      421 gtataatttc aatttaatcc tattcgtatg tcctaatctg gggtttagat attctacctt
      481 tcacctctta actatattaa atattcagcc atttatgttt atataaatgt attctcaaag
      541 acttcccgga tcattctata gtaacccact tacctggtac tcttgcagaa atggtattat
      601 catacatcgc ggaacaggat gcggccggca cataactctt acctgcataa gctgcaataa
      661 gctttatttt aaaactcttt tcagcacctt gatacaagct gaaaaatgtg tttacccgat
      721 catctctgat atcctgatag tctaaatatg atgattctga tgtcgccatt cctgcctgca
      781 ttctgtcatt gacgatctcc catccggatg gaaatatctg tgtcaatgca aggttttgaa
      841 tttgaaattc atagggtgct gtatttttta ctgtggcaat agcgtaaaat tcagttccct
      901 ttttcagctt aacaggatca attcgatttc ctgacttgtc agtgtacttt atagaaaggg
      961 ccaaactgta gtcaatagca gtctgatcgg tcaaggcggg ttttcctttc tgaaccatgg
     1021 ttacataaag gacaccctta cttttgttga caaccttaga ccctgccatt tttacctgag
     1081 agccaaattc aaccatatgt gcagcactgg attcgttgac ttccacagtt ttaccattga
     1141 ccgtatagct cgccttaaca cttccagctg gaggatattt agccagataa gttgctactg
     1201 cccaaatgcc atatgactta ctgtaggtag cataccaata gccgccggaa agtttttttg
     1261 cgattgattt agcaacggat gcggcatcct ctttccgttc ggccaaagta agtgcattta
     1321 acatgattgc ttcatccctt aagtccgaac caaatgaata gccaaattca cgataaggct
     1381 tagttgataa ttctgctttt tcaataaggg cagccgatac atctttcttt ccggaaagag
     1441 aataagctgc tgaaaggtaa tatcttgcag gaacgctgat tgtattcaat tccttcaatt
     1501 gattcatagc cccaatctca ggcgaccccg ccaaggcaag catgtataat ctatatgcct
     1561 gatcaatatc ggcattatgt gggtacaatc ccgctttcaa ttgttttggt tgccataatc
     1621 tagctgctcg cctttgtgaa gaaatcaagg tttcaagact tccttgagga acattgaacc
     1681 ctgccttttt agcctccaac ataaactgac cggcatagat actagtgtaa gggtcatcaa
     1741 agttacttcc aggccagaaa gcaaaccagc cttccggttt ttgaaatgtt ggcaatttgt
     1801 tcattgccac ttcaaccatt gacttgatac gatcctgatc agccttgctc agcacggtca
     1861 ggttcttcaa aaataattga ggaaaggcct tactgatagt ttgttccaga cacccatacg
     1921 gataatctat caattcagtc aaattacgtg acaaatcaat cgaagggaag gtagaaaact
     1981 ctaatgaaga ggtaacggaa ctttccattc ccgaaagcaa attctgtaat tgaacactct
     2041 ggcctgcgtt caatacatat tcctttgaag taactatggg caagtttgga ttacgcacct
     2101 ggatctcaac tgtttccgtg gaagattcat ttcctgaggt agcagttacg tcaatggatg
     2161 acacaccttc ctgcttagag acagtaacag gaaaatatac cattttctca ccactcgact
     2221 cgaatgtaag tgacttactt gacgaaccgg tcacagacat gtaaccattt ttgtcattta
     2281 tttttaccgt gacatttctg atgttattct tggtaacaaa tacatttacc ggcaagttga
     2341 aagtctcacc agggctcaat acacggggca aggtagttgt aaccataagt ggtttgacca
     2401 ccttcacgga ttgctgtgca gctccgaaag ccttatcatt acatgccact accatggcac
     2461 gcattgatcc tatgtaattc ttgattgtaa agtgatgcct tgccttctcc ccttttttca
     2521 acttaaaagg accgagattt acaacggcag gaacaaaacg attggccttg ggagctcctt
     2581 ctatctgtgc tgcagccata tctcctccta cagcgaagat accattcaat ttgccactgt
     2641 aagctcccaa aacataatca aacaagtccc aggtccttac agcaagggca gacttggcat
     2701 aaaagaaatt atagggattc ggagtcttga atttagtgat atccagtagt ccttcatcta
     2761 caacagcaat ggtatagtac atttcattac cattttgctc actcacggat atttctgcct
     2821 tcttatccgg ctgaatctct tgtggcatac taataacagg attgagtacc agtgccggat
     2881 tgacaatttt aaacggaagt actccataca ttctcaaagg catatcattt tccggatgtt
     2941 tatgaggctg cattaatgtc acaaaggcgt aagcattaga ccccatggca tcagttgtct
     3001 ttatcgaaac ttcgttgact ccctccttac aatcaaacca tttatgttcc actatggaac
     3061 ttcctccttc aatagtcaca agagccttgc ttccagctgc tgcagtaaag cttagattaa
     3121 ttgtctctcc gactttaact tccttggaat cgattcccaa aggtaatgaa gtcgcaatac
     3181 ttgacatttg ttccgcatcc gcataatccg gatatcctga ataaatgaat gttccggcta
     3241 catgcggact ggcatctgat gttactttaa ttaaatacct tccccaggca ggagggcgga
     3301 aatcaaacat aatttttcca tcccccttgg ttttgcctgt ataatgagct aacggcacct
     3361 cgttgaaatc accggtataa taattagcgt cgctgtaatc actctcccac caccatctcc
     3421 aatcgacttt atatacatta atagtagcat tgattccgga aagaggattt ccattttgat
     3481 cgaccacaat cgcactaatc ggagcatttt tcttatccgg aatcgaaggt tctccccatt
     3541 gattgtgtgg cacagaaact ccgacatata ccttgaatgg agagtaggtt tcagatgtgt
     3601 aatctacact gaaattgcct ccctgctcaa aaacggttgt cttaaaaacg atattactaa
     3661 tctggtgtat ttcctcgttg agattgatgt tcatttttaa ggaagcatta cctgaagcat
     3721 ctgtatttcc ctcaaatact tttttctcaa acgtcttccc agatgaggaa tttgaaaaaa
     3781 taaaatcaga atatttggta aaaggagtac tctgaactct gatattggca tccacgactg
     3841 ccctaaggtt tccagccact gtgccgatga gccaggcagc attcagactg gcattgacca
     3901 gtggctgccc tgccatcaac tgttttttgt cgacatcaaa ttttattttt agtcgattgg
     3961 gcttgacagt ctcaatactc atgagcttag taaaagttgc tcctccaacc ttaacggtag
     4021 ctgcccaaac tccggtagga gcatccggag atgtcgccaa atgaattggc agaatagagc
     4081 ctgaagtata attgtatgtc ttacgagcaa taatcgtatt tcgtggatcc cgccattcca
     4141 attggatcgg gtgtgaggca ggcaatgtct ttaatttatc ttctatgata caattgagaa
     4201 aaattgaatc tccgggacgc catatgcccc gttctgcata aagatatccc ttcaaccctt
     4261 tttggtaaga atcaccacca acgtcaaact tgcttaaaga aagggagcga ccatcatcca
     4321 acaataggta acttttatcg ccatcctttt cagcaattgc catgaatggt cttgatttaa
     4381 gggtaatgtt caggaaaccc tctccattac tcaaaccttt atcgatcacc tgttgctgaa
     4441 aatcataaaa ctttatctcc accccggata gtggctttgc tgtaagcaaa ttagttgctg
     4501 caacaaaaaa attgccgttg tcatttcctt tgacgataag gccaatattt gatttatata
     4561 cactttgagc gataaaatta tctttattat agtacatcgg tttacaggga ttttccctat
     4621 tcagccaatt ataatcctca tcgtattctt cagcaaatcc atcgtaatta tggtcatata
     4681 tactaacagg ctcttctccg taagcaaccg gctctgtact taactcgcgt tccttccaat
     4741 cccctcctga tttacataaa taagaagtat atgctctgcg gaatccaatc ctgacttgat
     4801 agatagaatt gcgatctgta gttatcaatt tattcagatc caaaccatac ctgacccacg
     4861 atgacttatt tatggctgaa ttaaggctgg aaagctggat tttctgttgc aggattatcc
     4921 tgccaacccg gttgatttca tagctatctt catatccatt atagttttga taatattgaa
     4981 gtacattatc tgaaaatatc ttgacgattt ctacatccac tgcattaaga ttgattgctt
     5041 caaaaggcaa aatcagacca tcgctctccg gcattatgtt ccctttgccg gcaaatctaa
     5101 ctgcaggttt ggtgtcgaga aaattaacta ccttactcca gggcttgccc aatgattcat
     5161 tccttgaatt caaaaccgaa ggtgcaacac ggacagtctt ctccccggtt attcgcttgg
     5221 atggatagac aagaatattg ttgttttgaa cagagtagtt cactacctca tcaaatccat
     5281 cgatagtgat catacctcca aggttctggg tagccgatac cggatctgta aaatttataa
     5341 taaagaaaaa atcctcatcc tggctaaaat aaatatcatg aaccttaaac tccgtcaaag
     5401 gagcaacagg aacctccctt gtaattacat tgttaactcc tatcggctta ccatcaaacc
     5461 ggatctccat tttttgatcg gaagaattac gcttaatacc gcgaatcgta aaattatgtg
     5521 taaccccctt tgagtcgtga ttccatgcta cacttagact ttgccctttg atggaagccg
     5581 tcagcaaggc ctcaatcttt gaagcttcga cttcatcagc cgtaagaatg gcgcccttgt
     5641 aagtttgctc agtgtaatca ctttgtgagg gggtatccca accggattcg gtgatggccg
     5701 catcctgtcg aatggtccgt atattaaaat tgaaatgcct gaattccttg ggaacattat
     5761 cctgcaaggc accaatgtta atgctgaaat catattgttt gtcactgaca aattcatcca
     5821 ttggtgtaaa cacaattgtc ttttcatctt gccaaaccgc atttcccttg acagaaggag
     5881 agaccgaaac cgctcctgaa gcaacaaaac caccgaccga cttggaatct accatattat
     5941 cggcaaacac aaccttcaca ggctgctttc gtgagattac gccactagta ttggcatata
     6001 taaaaggatt cttctcgttg ctttccgtat tcttcttaca tgctaaaaaa ctcgttgaga
     6061 aagccaagat aattagtgga taaagaaaaa cttttggtgc catataagac cggtttatta
     6121 atgattgaaa cattgattag aacgtatacg cgatggaaaa agtctcaaaa tcggatactt
     6181 taaatactct ccacgattca gctgaaatta ccaccacgtg gttccggtaa ttgaactata
     6241 aataaaaagt ccccccaaga cccaattata aaacaacaac cctcagttag gacaaacgca
     6301 caaccacgat agacatcaat agattgacgt gtcatatcat gcacggaccg gaaaactacc
     6361 gaaatctata gccgtcaatc aaaaagcatc ctacaaaatg aaacagtaac ttagtcttta
     6421 tcccaaaggt agcaagagtc tttgatttga cgaaaaataa ttatttttca tatattgaag
     6481 tatcgtttgg aaagttcagg catttttttc tatttttggg ccattaaatg ttataccatg
     6541 agtcaggaag aaaacaaaac caatactaaa acacaatgga tgatttttac ggtatcaacc
     6601 atagcgctaa ttttgctact tgtatacctg ccatcatttt tctgggtggc tctgcccttc
     6661 cagctgacat catttgctta tgcgatggac tgggtctaga ataccaaaat cacaaagtgt
     6721 gccaaaatat aagggagcct gtcaggttcc cttttttatt ttattcttgg cttctggtgt
     6781 aagtgaatat atatacctgt tactttagaa tcagctcgta tcctgattct aatgtatgcc
     6841 tcctaccctt tcttttattc ttgtaacaac cccattctga aagaaatcat agttgtattt
     6901 cagatagcta ttttcgtcct gataaataat taaccggact ccaatacatt ataagacaat
     6961 aagctgttga atatcagtaa tttttcggcc gcaattacat ttattcgcta aaaaaacacc
     7021 aaactgcacc tttttccccg tcaaaacctg caattttcct aaatacagtt tgtgctaaag
     7081 gatttttaat aaataattta tataatatat atattattga atatacaggc tattaaagca
     7141 aaataagaac caaattaaca ggcaagaggt tataaatccg atatatcttt taaatttgca
     7201 aaaaacgaat acaacgaaat ggaggattta atcctggaat tgaaagaaaa gctaattgct
     7261 caactcaatc ttaaacatct gacccctgaa gaaatcgata gtgatcaacc tttgtttgga
     7321 gttcgtattg ggctggattc aatagatgca ctggaaatca tcgttatgct tgataacgac
     7381 tatggcatca aattgacgaa tcaggaggaa gcaagggaaa tatttacatc cgtaaggaca
     7441 atcgctaact atattcataa gcaccaagcc aatgcctgaa tcccgtgttg tagtaaccgg
     7501 aatgggcatt atggcacctt tgggccgggg cgtatcggcc aatcatgctg ctttagtcga
     7561 taaccgtcat ggcttgggag atataactat cctgaacact atccacagag gaagtttgcc
     7621 tgccggtgaa ataaaattga caaatacaga ccttgcatcc attgccaact tccagggcga
     7681 gaacatccct cgatctgtgt tgctgagtgc ggttgcaatt caggaagcct tagcttcagc
     7741 cggattgcat cataatcatg atatcaattt ttactattcg actactgtcg gtggcatgga
     7801 tatatcagag gaattgaacg acaaaaggtt aaaaggtcaa gatattgact ttagtggctt
     7861 taagtatcat gactgtgggg ctagtacaat gcttttggga gacttttttg gttttacagg
     7921 tcgacgttac agcctttcta cagcatgttc atcatcagcc aattgtattg gcagagcaat
     7981 gatggatatc aagacgggtc gtgcccggta tgcagtagcc ggcggagcag atgcgcttag
     8041 ccgattcacc ttgaatggat tcaactccct gatgatattg gataaggaat tgtgcaatcc
     8101 gatggatcaa aacaggcgtg gtctcaattt gggagaagca gcggcctttc tggtactgga
     8161 atcggaagaa aacgctgtaa acagaggtgc agaaatactg gccatcgtaa gtgggtttgg
     8221 caataccaat gactcacatc accaaactgc cagcagcgaa tcaggaattg gggctgtcct
     8281 tgcaattcgg gaagcgctga acgatgccgg gttgaaagct gaaaatattt cctacatcaa
     8341 cgcacacgga acaggcaccc aaaataatga tctgtcggaa ggaaatgcat tggaaaccgt
     8401 ttttggtaag gtccccccca cagcatctac caagtcttat accgggcata ctttggctgc
     8461 ttcaggcgct gtagaggcta tctattctgt tttagccatc aaaaaccagg agatgtatcc
     8521 aacactaaga tttaaggaag cgatcgttgg tcacggcttt aagtcattcg attttaccat
     8581 acagggagag gttaaccacg tcttatccaa tgctttcggt tttggcggga attgttctgc
     8641 tttgatcttc agtaaatatt aatcaatgaa agcttacatc aatcacatca atgccatcag
     8701 cagataccaa tacctcgaag gttcaattat cgagcaaagc gagattatcg aacctgacta
     8761 tgaatcaata ctggacgtac gtgctatgcg caggctgagt aaaatcctga agatgggact
     8821 ttttactgca ctttctgtaa agaatcaagc taacaggcaa ccagatggaa tcatctgcgg
     8881 taccgctttg ggttgtctgg ctgatactta ccagtttttg aaaaatatga gtgaccgcga
     8941 tgaacagctt cttactccga ctgcattcat acagtccaca cataacacac tttccggtac
     9001 tttaggaata atgctgcaaa atcatagcta caatataacc tggagtaatc gggaattttc
     9061 attctacgac gcacttgaag atgctattat aagagtcaac aagtgtactg acgaccatat
     9121 actacttacg gcggctgacg aaatgccgcg gcaggtggcc gagattatag acagcatacc
     9181 ccttgattgt ccggtcccaa tcaatagtga aggtgctgct tcc
//
LOCUS       DAOQQN010000078         9182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2837_length_9182_cov_5.669990, whole genome shotgun sequence.
ACCESSION   DAOQQN010000078 DAOQQN010000000
VERSION     DAOQQN010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9182
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2837_length_9182_cov_5.669990"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PLG24_04370"
     CDS             <1..888
                     /locus_tag="PLG24_04370"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HMZ24154.1"
                     /translation="WIRTRTGIKKRRIFNSPGKGASDLALPAVLKLLETTNTRPEEVE
                     LVIVATVTADHTFPDTANILCDKAGIKNAYGFDINAACSGFLFALTTAAQFVMTGRHK
                     KVILVGVDVMSSIVDYTDRATCIIFGDGAGAVMLEPVEDGTGLIDSVLKGDGAGRHVL
                     HMKAGGSVKPASHETVDAREHFVYQEGKHVFKHAVNGMASTVAEIMKRNDLSKEDVAW
                     IVPHQANMRIITSVAEQLDFPIEKVMVNIEKYGNTTSATIPLCLWEWKDKLHKGDNII
                     LTAFGGGFTWGSTLIRWSI"
     gene            1018..1581
                     /gene="efp"
                     /locus_tag="PLG24_04375"
     CDS             1018..1581
                     /gene="efp"
                     /locus_tag="PLG24_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HMZ24155.1"
                     /translation="MADTGDIRKGLCIDYNNDIYVVTDFQHVKPARGAAFVRTTLKSM
                     TTGKSLENTWPSGHKLDVVRVERRKFQFLYKDDNGLNLMDNESYEQITISEDMVDSPQ
                     FLKESMDVDILYHAEKDIPLRVELPQYINVQVTYSEPGAKGDTATNASKPATIETGAV
                     IKVPLFINEGDVIKIDTLTGAYIERAK"
     gene            1625..2107
                     /gene="accB"
                     /locus_tag="PLG24_04380"
     CDS             1625..2107
                     /gene="accB"
                     /locus_tag="PLG24_04380"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HMZ24156.1"
                     /translation="MTYKEIQELIRQVAKSDLAEVKVKDNESEVTIRTRYYSEGKTSS
                     VTYSQAPSPVQQPAAMTQTVAPSTPKSEAEAPIETKSTGLKEIRSPMVGTFYRSPGPD
                     KAAYVKPGDTISAGTVVCIVEAMKLFNEIESEVSGTIVKVMVEDATPVEYDQVLFLVE
                     "
     gene            2167..3507
                     /gene="accC"
                     /locus_tag="PLG24_04385"
     CDS             2167..3507
                     /gene="accC"
                     /locus_tag="PLG24_04385"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HMZ24157.1"
                     /translation="MFNKILIANRGEIALRIIRTCKEMGIKTVAIYSTADRESLHVRF
                     ADEAVCIGPASSKDSYLNIPRIMAAAEITNVDAIHPGYGFLAENADFAEVCTQYGIKF
                     IGPTPEQIRKMGDKVTAKETMIAAGVPVVPGSDGLVEDVKEGKKISKKIGFPVIIKAT
                     AGGGGKGMRIVWSEEEFEDNWNMARNEAKNAFGNDGIYIEKYVEEPRHIEFQIIGDQF
                     GRVAHLSERDCSIQRRHQKLVEESPSPFMTPELREVMGTAAIKAGKAINYEGVGTIEF
                     LVDKHRNFYFMEMNTRIQVEHPVTEEVIDHDLIKEQIKVAAGIPISGKSYLPSLCAME
                     CRINAEDPFHDFRPSPGKITSFHSAKGHGVRVDTHVYAGYTVPPNYDSMIAKIICKAQ
                     TREEVILKMQRALDEFIVEGIKTTVPFHQRLLRDPNFIAGEFNTGFLNHFDLTE"
     gene            complement(4186..4488)
                     /locus_tag="PLG24_04390"
     CDS             complement(4186..4488)
                     /locus_tag="PLG24_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMZ24158.1"
                     /translation="MKKTKVTIDNDKLQISSEIMRALAHPLRLKILEFIDKHQAINVN
                     KIYNTLKIEQSITSQHLRILRNSGVVITEKSGKFVNYHINYEVVNKAVIAVNNFYA"
     gene            complement(4734..5072)
                     /locus_tag="PLG24_04395"
     CDS             complement(4734..5072)
                     /locus_tag="PLG24_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMZ24159.1"
                     /translation="MRKTKNDSISLKGGKSEVQLDYTALRKAVLVLRAVNHKLRQRII
                     DLLEEGDNMTVTDIYIKLRLEQSVASQHLAILRRSGVVVTDRQGKFIYYSLDKDRLAQ
                     ISRLVEELAS"
     gene            complement(5247..5951)
                     /gene="tsaB"
                     /locus_tag="PLG24_04400"
     CDS             complement(5247..5951)
                     /gene="tsaB"
                     /locus_tag="PLG24_04400"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HMZ24160.1"
                     /translation="MKTFLAIDTISVPFSIAIGNQESVLAEEILFDRMALAGQITLMV
                     DRCCKKAGLDLSSLSGILISKGPGSYTGLRIGYSIAKGLCMAMSLPLIEVDPMMAMAF
                     VSMEQTGDALNPVFCSALDARRNEVYLAIYNKYGREILKPQPCILPIELKDLIGDIRP
                     DLICCSGDGALKLEGQLGDWNYRTTGICSIASYLFKVMDAGVEHPDFDSIASAEPLYL
                     KPPNITISKKKMPGGR"
     gene            complement(5953..7590)
                     /locus_tag="PLG24_04405"
     CDS             complement(5953..7590)
                     /locus_tag="PLG24_04405"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HMZ24161.1"
                     /translation="MDSYKGVKTHFFSGLVILWIFLLCLTLGAPLLSAQEIASTDLKL
                     FHYSHFINRQELRTHVGLLASDSLEGRGLGTFGSRKASEYIAGQLKNEGLYPIGDRET
                     YFQPVVFKQWYRDKAEFLLTLPDGKIRGQLMPGRDFTFNLNELPMPYSLSADHLMFAG
                     YGIADDAYNDFKYAQAWDEVLLILDGEPMEKGKYVISNTIQPSSWSLGLDRKLQTAVH
                     KGAKAVILIEDSTTYVKRGASEYPGRGKFAEQIVYNYPIPVIRVSEAALVRLFAPKEH
                     ARLAGMIARVKRGDVASNYVQGGFKIDLSITHTLVRDRNVVGLIEGDDPRLKHEYIVL
                     SAHFDHLGIINGEIFNGADDNATGTAALISISKALKALKDNGYPLKRSVLFLFCTGEE
                     SGLLGSRYFVNNPLVPLKHIKADINIDMIGRRDTLHAEKENYLYVIGSDKINPLLDDI
                     LNESNDKTIRYGLDYTYNNVDHPLRLYYRSDHYNFAEKGIPSVFLFGGFHKDYHRPSD
                     DTMLIDFKKVEDISKLVFFTTVGLSNYSGEIKTDYKP"
     gene            complement(7899..>9182)
                     /locus_tag="PLG24_04410"
     CDS             complement(7899..>9182)
                     /locus_tag="PLG24_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HMZ24162.1"
                     /translation="PTVMAFQNYDDMGRRGIYASRDYAANFLNSVGRDAIIFTYGDND
                     TYPLWYAQEVEGIRPDVRVVNLSLIAVDWYIEGQRRKINESPYIKMTIPSDSYRGNKR
                     NQVYYVDTELANQELPAEGVLRFIGESHPLEGSGSKAESFLPTKNVYIPIDKAKWINK
                     KFLSPQDSILDRLPLSLGVKEQGDYILKGDLAVMDIIASNINDRPIYFSVTADPSKFL
                     GLNNFMQLEGLALRIVPVRSNSDRQFGVFGTGRVQTDTLYNNVMKKFRWGNFDKKKLF
                     VDRSYTPTVNALRMIQMRAAFDMISKGDKKRAADLAENTLKVFPNMNFPFDGAIIPAL
                     SMLTTADNIDRAKPYMKQLAENLREKMVYFHSMTPVELSEGYQADKEETESAIADLMD
                     MIGRSKDQGFEEEIRKIIGPYIPKADGAIPKMDER"
BASE COUNT         2580 a         2073 c         1931 g         2598 t
ORIGIN      
        1 tggatccgta cccgaaccgg catcaagaaa agaagaatct ttaatagtcc cggcaaagga
       61 gccagtgatc ttgcattgcc ggctgtcttg aaattgctgg agacaactaa taccaggccg
      121 gaagaggtgg agttggtaat tgtggccacc gtgacagcag atcatacatt tccggacact
      181 gccaatatcc tttgtgataa agcaggaatc aaaaatgcct acggttttga tataaatgcc
      241 gcatgcagtg gatttctttt cgccttgacc accgcagcac agtttgttat gaccggtcgg
      301 cacaaaaagg tcatcctcgt cggcgtagat gtcatgtctt ccattgtaga ttataccgac
      361 agagcaactt gtataatttt tggcgatggc gcgggtgccg tcatgctgga gccggtggaa
      421 gatggtaccg gccttattga ttctgtttta aagggtgacg gagcaggcag acatgtcttg
      481 catatgaaag caggaggctc cgtgaagccg gcctctcatg aaactgtcga cgcccgtgag
      541 cacttcgtat atcaggaagg taagcatgta ttcaagcatg cagtcaatgg catggcttct
      601 actgtggctg aaatcatgaa acgaaacgac ttgtcaaaag aggatgttgc atggattgtt
      661 ccgcatcagg ctaatatgcg tatcataacc tccgtagcag agcaactgga ttttccaatt
      721 gaaaaagtca tggtcaatat agagaaatac ggaaatacaa catccgcaac catacctcta
      781 tgcctgtggg aatggaaaga taaattacac aagggggaca acattattct tactgcattt
      841 ggcggtggct ttacctgggg atcaaccttg atacgatggt ctatttgaac agcatgaagc
      901 tttcgataaa gtttcttact cataaattta aataaccgaa ccaatcaatt ttttttaacg
      961 agataataaa aaagcattaa ttttatagct ttgcgcccga attaaaaact ataatttatg
     1021 gcagatacag gagatatcag aaaggggctt tgcatcgatt acaacaacga catctatgtt
     1081 gtgacggatt ttcaacatgt caaaccagcg aggggcgcag catttgtccg gaccacgctt
     1141 aagagtatga caacaggaaa gtccctggag aacacatggc cctcagggca taagctggat
     1201 gttgtgcgag tggaaagacg aaaatttcag ttcttatata aggatgataa tggcctcaat
     1261 ctgatggata atgaatcata tgagcaaatc actatttctg aagatatggt tgatagccca
     1321 caatttctga aagaaagtat ggatgtggat atcctttacc atgccgaaaa agatattcca
     1381 ctgagggtag aattacctca atatatcaac gtgcaggtaa cgtactcaga accgggcgcc
     1441 aaaggtgata cggcaaccaa tgcaagtaag ccggcgacca ttgaaaccgg agctgtgatc
     1501 aaggttccat tgtttatcaa tgaaggcgat gtaatcaaga ttgacacatt gaccggtgca
     1561 tatatcgaaa gagccaaata ataacggaac tcacccgcta accctaacaa acaaatttgt
     1621 tgacatgacc tacaaagaaa ttcaagagtt gattcgccag gtggcaaagt ccgatctggc
     1681 agaagttaaa gtcaaggata atgaatctga agtaaccatc aggacccgtt attattctga
     1741 aggaaagacg agttcggtta cctactctca ggctccgtca ccggttcagc aaccggccgc
     1801 aatgacacag actgtcgctc cttctacacc aaaatcagag gctgaagctc cgatagaaac
     1861 gaagtcaacc ggactaaagg aaattcgttc tcccatggtt ggaaccttct atcgctcccc
     1921 cggacctgac aaagcagctt atgtcaaacc gggcgacacc atttcagccg gcaccgtagt
     1981 gtgtatcgtt gaagcgatga agctgttcaa tgaaatcgaa tcggaagttt ccggtaccat
     2041 cgttaaggtc atggtcgaag atgctacccc tgtagagtac gatcaggtgc ttttcttggt
     2101 cgagtaaagg atttcgggat ttattttaac ccgccttcac atctattcat caacagaatt
     2161 gttcttatgt ttaataaaat attgattgcc aacaggggtg agatcgctct caggattatc
     2221 cgtacctgta aggaaatggg tatcaagacg gtggctatct actcgacggc cgacagggaa
     2281 agcctacacg tgcgctttgc agatgaagca gtctgcattg gccccgcctc atcaaaggat
     2341 tcttacctga acatcccgag gatcatggca gccgctgaaa tcactaatgt ggacgccatc
     2401 catcccggat acggattttt ggctgaaaat gccgacttcg ctgaggtctg cacccaatac
     2461 ggtatcaaat tcatcggccc tactcctgaa caaatcagaa aaatgggcga taaggtaact
     2521 gccaaggaaa ccatgattgc cgcaggagta ccggttgtgc ccggctctga cggtttagta
     2581 gaggatgtca aggaagggaa gaagatcagc aagaaaattg gcttcccggt catcattaag
     2641 gcgacagcag gcggtggtgg caagggtatg cgtatcgtat ggtccgaaga agaatttgaa
     2701 gacaactgga atatggcacg taatgaggcc aaaaacgcat tcggcaacga cggtatctat
     2761 attgaaaaat acgttgagga gccccgccat attgaattcc agatcatcgg agaccaattt
     2821 ggccgggtgg cacacctgtc cgaacgcgat tgctccattc agagaagaca tcaaaaattg
     2881 gtcgaagaaa gtccttcccc ctttatgacc cctgaactta gagaagtcat gggtaccgct
     2941 gccatcaagg caggaaaggc aatcaactat gaaggcgtgg gcactattga atttttagtg
     3001 gataagcacc gcaacttcta cttcatggaa atgaataccc gaatccaggt ggaacatccg
     3061 gtaacagaag aggtcatcga tcacgacctg atcaaggaac agatcaaagt tgcagccggt
     3121 atacctattt ccggcaagtc ctatttgcct tccctttgtg cgatggaatg caggatcaat
     3181 gcagaagacc cattccatga tttcagacca agtcccggta agatcacatc cttccacagc
     3241 gccaaaggac acggtgtgcg ggtcgatacc catgtatatg ccggctatac agtacctccc
     3301 aattacgact cgatgatcgc caagatcatt tgtaaagcac agacccggga ggaagtaatc
     3361 ctgaaaatgc aacgggctct tgatgaattt atcgtggagg gtattaaaac taccgtgcct
     3421 tttcaccaga gactgttgcg cgatcctaac tttatcgccg gggagttcaa tacaggcttc
     3481 ctcaatcatt ttgacctgac tgaataatct tcagattgaa agataacaaa gctctgcgtt
     3541 tgacatcgca gagctttttt tgtcccgttc ctattgaatt caatcatcct gccatgatat
     3601 tgatggggcg gattatcaca aatggacgca tccggctact cctatcttct gcaacgactt
     3661 ctcatttgct caatagagaa gggaggtttg tcgtacaccg aatgtaatcg tttccgggat
     3721 atttcctttg cccgcacata agccaccctg ttctaatctt ggccatttga acaacgaaag
     3781 aaatttcttt gtatcctttg aaacaccaaa agccgtaata actaaagatt ataaagttca
     3841 agtgatcaca aacgatgtga cataacgctt gtgtcaatat ggtcatcgcg gacttcagga
     3901 acaaccggtt gcacaggtca aatacctgcc attcggcatg aatcgataac gaaaaagagg
     3961 cagaacggaa tgatgtccct gatcccctca atgcaatgag gcgtcataga ggacaaagac
     4021 taaaaagatg ctgccaaaaa cacatgacaa catcttttta atcttataat tgttgagaga
     4081 atccctaagg tcatctcgtt cgggtagaaa tttcctcaat atttttaatt ggttactgtt
     4141 tttttgatct gaacgttttg tctgagttcg atgggatgga ggaaatcagg cgtagaagtt
     4201 attcactgca atcactgctt tgtttacaac ctcataattg atgtgatagt tgacaaactt
     4261 acctgacttt tcagtaatca cgaccccgct gtttctcagg attctcaaat gttgagaagt
     4321 gatagattgc tcaatcttca acgtattgta tattttgttt acgttgatag cctgatgctt
     4381 gtcgataaat tccaggattt taagccgcag tggatgtgcc agtgcccgca tgatttcaga
     4441 tgaaatctga agtttgtcat tgtcaattgt aaccttagtc tttttcataa aaatattttg
     4501 tagcgtttct taataaatca ccttctatcc gagtcatccg actttgatat tcagttgtag
     4561 tttgaccttt atgccacaaa tttgaaacaa attttctttt ttgcaaaaaa attgccacaa
     4621 tttttttgaa ttgtggcaat ttttttttaa aattaagggc ggctttaatg taatccttaa
     4681 attaagattg tatatttatc tgataatctg ttgtttgtac ttgtttgtca aatttaactt
     4741 gcaagctctt cgactagtct ggaaatttgt gcaagacggt ctttatccaa agaataatag
     4801 ataaatttac cctgacgatc tgtaacaaca acacccgacc tgcgcaaaat agccagatgt
     4861 tgactagcaa ccgattgttc caatcttaat ttaatataaa tgtcagtaac agtcatatta
     4921 tcaccttctt ctaaaaggtc gataatccgc tgcctcaact tgtggttgac agcacgcagt
     4981 accaatacag cttttctcaa cgctgtatag tccaactgca cttctgactt gccacccttc
     5041 aaggaaatag agtcgttttt agtttttctc atattcaaaa tgcatttagt tttggattat
     5101 accatttacc taaccaaaac tgtaccaaat tttttcaaat tgaaatttat tattatatga
     5161 gaacaaataa attattttta tgtttgtcta tcaggcgtat atatattatt tctggggtta
     5221 aattttttat agcccgacat ttctgctcat cttcctccgg gcattttttt cttggatatg
     5281 gtaatgttag gtggtttgag gtaaagcggc tctgcgctcg ctatagagtc aaaatcggga
     5341 tgttccactc ctgcatccat gaccttaaac aggtaggacg ctatactgca gattcctgtt
     5401 gtacgatagt tccaatctcc caactgacct tccagtttta atgctccatc gccgctgcaa
     5461 caaatcaaat caggacgaat atccccaatc agatctttga gctctatcgg aaggatacat
     5521 ggctgaggtt tcagaatttc tcttccatat ttattatata tagcaagata tacctcattg
     5581 cgcctggcat ccagagctga acaaaaaaca gggttcaatg catctcctgt ttgctccatt
     5641 gatacaaatg ccattgccat catcgggtcg acctcaatca gaggaagact catcgccata
     5701 cataatcctt ttgctatgga atatcctatt ctcagaccgg tataactacc ggggcctttt
     5761 gaaatcaaaa tgcccgacaa gctgctcagg tccaggccgg cttttttaca gcaacggtca
     5821 accataagtg taatctgccc tgccagggcc atacgatcaa acagaatctc ctcggccaat
     5881 acgctttcct gattgccgat cgctattgaa aaaggaaccg agatggtatc tatagcaaga
     5941 aaagtcttca tatcagggct tgtaatcagt tttgatctct ccggaatagt tggataatcc
     6001 aaccgtcgta aaaaacacga gtttgctgat atcttctacc tttttaaaat ctatcaacat
     6061 ggtatcgtct gaaggccgat ggtaatcctt atggaagcct ccgaataaaa acacacttgg
     6121 aatacctttt tcggcgaaat tataatggtc gcttctgtaa taaagcctca aggggtgatc
     6181 gacattgttg taagtataat ccagcccgta tctgatggtt ttatcgttac tttcattcaa
     6241 tatatcatca agcaaaggat taatcttatc cgacccaatc acatataaat agttttcctt
     6301 ttctgcatga agcgtatccc gacgaccgat catgtctatg ttgatgtctg cctttatgtg
     6361 tttgagaggc accaggggat tgtttacaaa ataacgggaa cccaacagcc cggattcctc
     6421 tccggtacaa aagagaaaca agacgcttct cttcaacgga tatccgttgt ccttcaatgc
     6481 ttttaacgct tttgaaatag agatcagcgc tgcggtaccg gtggcattat catctgcgcc
     6541 attgaagatt tcaccgttta taatacccaa atgatcaaag tgggctgaca gtacaatgta
     6601 ttcgtgtttc agcctgggat catcaccttc tatgagtccc accacatttc gatcccggac
     6661 taatgtgtgc gttatcgaca gatcaatttt aaatccgccc tgaacgtaat tggaagccac
     6721 atcaccacgc ttgactctgg ctatcatccc tgcaagcctt gcgtgttctt ttggggcaaa
     6781 taatctcacc aatgcggctt cactcactct gatgaccggt atcggataat tatatactat
     6841 ttgctcggca aattttcctc ttcccggata ttccgacgct ccccgcttga cataagtagt
     6901 ggagtcctct atcagaataa ccgcttttgc ccctttgtgt actgctgtct gcaatttcct
     6961 gtccagccca agactccatg aggagggctg tatggtgttg gatatcacgt acttgccttt
     7021 ttccatgggc tcaccatcca ggattaatag aacctcatcc caggcctgag catatttaaa
     7081 gtcattgtat gcatcgtcag caatgccata tccggcaaac atcaaatgat ctgcactcaa
     7141 tgaataaggc ataggtagtt cgttgaggtt gaatgtaaaa tcacgcccgg gcataagctg
     7201 accccttatt tttccatcag ggagtgtaag tagaaattcc gccttgtccc tgtaccattg
     7261 cttgaaaact acaggctgaa aatatgtctc cctgtctcca atcgggtaga ggccttcatt
     7321 tttcaattga ccggctatat attcactggc cttgcgacta ccgaaggtcc ccagtccccg
     7381 cccttccagc gaatcggagg caagcaatcc gacatgagtc ctaagctcct gacgattgat
     7441 gaagtgtgaa tagtgaaaga gtttgaggtc cgttgaggca atttcctgtg ctgacaacaa
     7501 tggagctccc agagtaaggc acaatagaaa tatccataaa ataacaagtc ctgaaaaaaa
     7561 atgtgtcttt acgcctttgt atgaatccat attaattgtt tctcccttcg attaaatcct
     7621 gtccatttta ttttgttttg gccgggtcag tacaaactct gtatttactg tctcaaagga
     7681 ataataaatg agaatacttt tttacctggt cgggaaagcg gtctcaatcg attgacctga
     7741 tccctcccgg atcccttact aatagtttat tcaaattctg atcattacga cctaaacttc
     7801 gttaactctt tatggtacaa tgccggaaac atggcgctac cacatttccg gcattatgtc
     7861 cgggcaaaga tcagcaggcg aatggtgcca cccggtcctt atcgttcgtc catcttgggt
     7921 atagccccat ctgccttagg aatatatggg cctatgatct tcctgatttc ctcttcaaat
     7981 ccctggtctt tggatcggcc gatcatatcc atcaaatccg caatagcact ttctgtttct
     8041 tctttgtccg cctgatatcc ttccgacaac tccaccggag tcatactgtg gaagtacacc
     8101 attttttctc ggaggttttc agccaattgc ttcatgtatg gctttgcccg gtcgatatta
     8161 tctgcggtag tcagcatgga caaggccggt atgatggctc cgtcaaatgg aaagttcatg
     8221 ttggggaata ccttcagtgt gttttctgcc aggtctgccg cccgcttctt atcccctttg
     8281 ctgatcatat caaatgcagc cctcatctgt atcatgcgca aagcattaac tgtaggcgta
     8341 taagaacgat cgacaaacaa tttcttctta tcaaaattgc cccagcggaa ctttttcatc
     8401 acattattat atagtgtgtc tgtttgcacc ctgccggttc caaatacgcc gaattgtctg
     8461 tctgaattgg atctgacagg tacaattcgc agtgcgagtc cttcaagctg catgaagtta
     8521 tttaagccga ggaacttgga aggatctgct gtgacagaga aataaatcgg tctgtcattg
     8581 atattactcg caatgatatc catcacggcc aggtcgcctt tcaatatata gtctccttgt
     8641 tccttgacgc ccaatgacaa gggcagtctg tccaggatag aatcctgagg gctcaggaac
     8701 tttttattaa tccacttggc tttatcaata gggatgtata cgtttttggt gggcaggaag
     8761 gattcagcct tggatcctga gccttccagc ggatgagact ccccaataaa tctgagtacc
     8821 ccttctgccg gcaattcctg attggcaagc tctgtatcca cataatacac ctgattgcgc
     8881 ttgttacctc tgtatgagtc agatggaatc gtcattttga tataaggtga ttcattgatc
     8941 ttccttcttt gcccctcgat ataccaatcc acagcaatca gagacaggtt gacaacccgt
     9001 acatccggac ggattccttc cacctcctgt gcataccaca acggataggt gtcattatca
     9061 ccataggtaa atatgatggc atccctgccc accgaattca ggaaattggc tgcataatct
     9121 cgtgaagcgt agatgccgcg tcggcccata tcgtcatagt tctggaaggc catcactgtt
     9181 gg
//
LOCUS       DAOQQN010000079         9102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2874_length_9102_cov_5.568807, whole genome shotgun sequence.
ACCESSION   DAOQQN010000079 DAOQQN010000000
VERSION     DAOQQN010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9102
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2874_length_9102_cov_5.568807"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..1062)
                     /locus_tag="PLG24_04415"
     CDS             complement(220..1062)
                     /locus_tag="PLG24_04415"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HMZ24163.1"
                     /translation="MYNKIIISFLTLFLCLDLTSQSISEEGKLFIIGGGRKPASMIQR
                     MVKEANFKQGDYAMILPMASGAPHSAINEMKKALAESGIMECYGINFDQSMIKDPIKL
                     DSVKNARLIFLSGGDQNRFMHLVLNSPLEQAIKDALSNGALIAGTSAGAAMMSKYMIT
                     GNQLQDTVYTATFKNIIAENVEIAMGLGLLVNAIVDQHFLIRSRHNRLLTAICEYPGN
                     TGIGIDESTALLVKGNIGTVVGESQVLVYRLDSRPKVGKNGKFSVPKIRTEIYTEGDS
                     FKIK"
     gene            complement(1065..2198)
                     /locus_tag="PLG24_04420"
     CDS             complement(1065..2198)
                     /locus_tag="PLG24_04420"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ24164.1"
                     /translation="MKSILKLLLIVIMLNGIVSCDHDFPVPPASIVPKFTYVADNDGT
                     APSTVTFTNTSIVPTEYGAPTYTWNFGDNTTSTETNPVHRYENPGVYQVSLVIVATGT
                     GQIKTITQAINIKNPDASGVPIYFTNSTQVLQAYINDDAPVFEVLPVTNIKDSYGICI
                     DTIHDVLYIADLDGGAIIQTDLNGKNQKIWRSGLDSPSGMAIDYEKNELYWDTSNGIQ
                     KGDLNSDDVNQKVDFITGQTNDPDGIAIDPVNRNLYWINYDGGLGMKGLDGSGEKILI
                     PDAEGARIIVVGNRIYFDYYNGSGDIHIKSANLDGSNLATLATGISRVVYGLAYDPYG
                     NKIYWGDRNKGQITRADLDGSNVQTFYTAAGSTPRGIVFGKKK"
     gene            complement(2362..3468)
                     /locus_tag="PLG24_04425"
     CDS             complement(2362..3468)
                     /locus_tag="PLG24_04425"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HMZ24165.1"
                     /translation="MKSLKNLLPLFLLAVLAVTGCKKDDPVKPKPVASFTASQTTVFV
                     GDEVVFTNTSENATSYTWSFGDGTTSTDKSPKKAYTAVGEYTVNLTATGEGGTATASA
                     KITVTKPVSKKTLYYIELGDKNKISKVSLTEGSQAELFLDIVGKGGVGLAYDATNDKI
                     YYSDFENADEGIIWRMNGDGTGLEKIASGITDPYSIAIDLKGGKIFWADDNGNISRAN
                     LDGSNVEKSFINVADGQMRGIAFNSKTNKLYFYEVNNEDLYIANADGTGVAVLIPGVY
                     GYGIFVDEVNEKLYYDERNAQALMQASLDGTGAKTISSTGKTRIHGIAIDYDANKLYW
                     ADRDKGEIKRANLDGSGEESFLSGLDSPRGIFIK"
     gene            complement(3491..4819)
                     /locus_tag="PLG24_04430"
     CDS             complement(3491..4819)
                     /locus_tag="PLG24_04430"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HMZ24166.1"
                     /translation="MKRILILLVLVYSFSSCNVLDVEPTNSIPASEAFKTKADLNRGI
                     LGAYNSLQGLSYYGRAYLTVSDLSADNLLYPPSATTADYLDIDNNHILPENGVIEGIW
                     GVAYDGLNVANNVIAKIPEMSFLNQDEKNVALAELYFLRALNHFNLLNYFGGIPIKTK
                     PTIGLSEINAARNSPEEVYATIIQDLTFASTYLPKSSASKIRASQGAANALLARVYLY
                     KKDYADAVIYASKVIDDNNYSLMSNYSDIFAADESKESIFEIDFTSLDRNRIAEYNFP
                     LTLNGRGSVGPDPGIVSAYEANDKRKDASIAFAGTIPYAIKYHDLATGSDNVIVLRLA
                     EMYLIRAEANARLNGNINDIRKDLNTIRSRAGLEEAVANSIDELLLAIEAERRVELAF
                     EGHRWFDLVRTDRAIDLLPNVTQKYQYLFPIPQSELLANTNPGMKQNPGY"
     gene            complement(4821..7769)
                     /locus_tag="PLG24_04435"
     CDS             complement(4821..7769)
                     /locus_tag="PLG24_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ24167.1"
                     /translation="MTRYVLKQGLVLLLLFLFGNLHGQISGTVRDASGQGLVGVTILA
                     IGENNGTVTDIDGNYSLRVNTANALEFSYVGYQTQKINIDGRKMIDVVMQEDVKALKE
                     VVVVGYGVEQKKTLTSSVGIVNSDAIKDLPVPTIDGVLQGQASGVQVVQNSGTPGGGM
                     SVRIRGVSSISGSSQPLYVIDGIPVTTGDFAQVGYEGQGVNQLSDLNPGDIESITILK
                     DAAAATIYGARASNGVVLITTKRGSSKKSTINLSASYGWQQPWRKLEMLNAHDWKLYR
                     NDYFGTEVYTPEQINDNTIDTKWQDYIFRTAPISNYELSTSGGDEKTRFYISGNLFNQ
                     TGTLIGSDYRRMNGRVNLDHKINNSVTIGTSIGLSYAKTNRIEGDQSLHGVLPNGIST
                     PAVFPVYNSDGSYNQDGPYSNPVSIANEATNENFSYRGLGNIYLDWKIWKGLTFSTKW
                     GIDFMNFREHAFEYNTVQGRKYNGLGFETYTNVGNLVSNNTLKYSFDLQDNRFEVLAG
                     YSFERKNVASSYIRGQDYADASLQYINSASTITSASSGASNEGIRSLFGRINYNLKDK
                     YLVSLTGRMDGSSRFSENNRNGFFPAVSAAWRLGEEDFFKSNVISELKVRGSFGLTGN
                     DDIPQFLYAELYGNTTYGGKPAVYPSNIPNPDLKWETTTQVNAGLDIGLFKDRIYLNI
                     DLYRKFTKDLLLSRPLPLSSGFSTITENIGEVENKGIELGLTTENLADRKLEWNTRIN
                     FSLNRNKITKLYNNQPIDDLGRGSNRIEVGQPIGIFYSYEWLGVDPSTGDVVFADKNF
                     DGNITSDDRTIIGNPHPDFIYAITNNFKYGRFDLSIFFQGSQGNDVMNGSRLYLESLQ
                     GGDNQLAAINRRWKNVGDITDIPRATNDPVAAASNKRLSSRFLEDGSYLRLKNLTFGY
                     NFNTKNIMKGKISSLRIYFTGQNLLTFTNYTGLDPEVNYVGDDNARMGTDFFTYPQAR
                     SFSFGLNAGF"
     gene            8139..8777
                     /gene="recR"
                     /locus_tag="PLG24_04440"
     CDS             8139..8777
                     /gene="recR"
                     /locus_tag="PLG24_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HMZ24168.1"
                     /translation="MKYSSQLIGKAVESFSSLPGIGKKTALRLSLHLLSKDAMWVQAF
                     SESFIKLRNDLKECDECHNLGDEVLCSICTDKRRDNGILCIVETIRDLMAIEETGHFQ
                     GFYHVVGGLISPIDGIGPDQLNIEDIEQRIINKNIREVVMAISPNIEGETTMFYIAKR
                     LQHLPIRISTISRGISFGGELEYADEVTLGRSIMARIPYMQFEPSTAEHGDR"
BASE COUNT         2593 a         1890 c         1717 g         2902 t
ORIGIN      
        1 cctgtaccgc tcagcaaatt catcgcctac tttttttatt ccggcaaaat tcattgtacc
       61 gctattgatg ttgacgattt tttttaaaaa tgccaaggct tcatcatgat tgcggtcaac
      121 tttttctact atttttcttt caattccgga aatttgacaa tcagccattg tagcccaaaa
      181 tatacatatt gtgatcagaa aaaatctact catcatcaat tacttgattt taaaactatc
      241 tccttcggta tatatttcag tcctgatttt ggggacggaa aattttccat tcttgccaac
      301 tttgggacgg gaatccagcc gatatactaa tacctgactc tcccccacta cggttccaat
      361 atttcctttg accagtaatg cagtagactc atcaatacct attccggtat ttccgggata
      421 ttcacagatt gcggtcaata atcgattatg tctgctcctt atcaggaaat gctgatctac
      481 tatcgcattt accaataatc ccaagcccat agcaatttca acattttcag caattatatt
      541 tttaaaggta gctgtgtata ctgtatcctg caattgattg ccggtgatca tgtatttgga
      601 catcattgcg gccccggcac ttgttcctgc tatcaatgca ccgttagaca aagcatcttt
      661 gattgcctgt tccaatggtg aatttagtac aagatgcatg aatctgttct ggtcgccacc
      721 actcagaaag attaaccttg cgttttttac agaatccaat ttgattggat ccttgatcat
      781 tgattgatcg aagttgatcc cataacattc cataataccc gactctgcca aagctttttt
      841 catttcattg attgcagaat gaggcgctcc actggccata ggaagtatca ttgcataatc
      901 tccttgtttg aaattagctt cctttaccat tctttgaatc attgaggccg gtttgcgacc
      961 accaccaatg atgaataatt tgccttcttc agaaattgac tgtgaagtca gatcaagaca
     1021 gagaaagaga gtcagaaatg atatgataat tttattatac acgattactt ctttttgcca
     1081 aaaacaatac ctctcggtgt gcttccggca gctgtgtaga aagtttgaac atttgaccca
     1141 tcaaggtcag ctctggtaat ttgaccttta tttctgtctc cccaataaat tttattccca
     1201 tagggatcat atgctaatcc atatacaact cttgagattc ctgtagcaag tgtagcaaga
     1261 ttgcttccat ccaaattggc agacttgata tggatatcac cggaaccgtt atagtaatca
     1321 aaatatattc tgttgccaac aacaatgatt cttgcccctt ctgcatctgg aatcaggatt
     1381 ttttctccac ttccgtccag gcctttcatg cctaatccac catcatagtt gatccaatac
     1441 agattacgat tgaccggatc gatagcaatt ccgtcaggat catttgtttg accggtgatg
     1501 aaatcgactt tttggttgac atcatcacta tttaagtctc ctttttgaat accattggaa
     1561 gtatcccaat aaagttcatt tttttcatag tctattgcca tgccgcttgg cgaatcaaga
     1621 ccactcctcc aaatcttttg attttttcca ttgaggtcag tctgaattat tgccccacca
     1681 tccaaatcag caatatagag tacatcatgg atggtatcaa tacaaatacc ataggaatcc
     1741 tttatattgg ttactggcaa aacttcaaaa accggagcat cgtcattgat atatgcctga
     1801 agaacttgag tgctattggt aaaatagata ggaacacctg acgcatctgg atttttaatg
     1861 ttgatggcct gtgtaatcgt ctttatctgg cctgttcctg ttgcaacaat aaccaaagaa
     1921 acctggtaga caccgggatt ttcgtaccta tggaccggat ttgtttctgt agaggttgta
     1981 ttatctccaa aattccaggt gtaagtagga gctccgtact ctgtcgggac aatggaagtg
     2041 ttagtaaaag ttaccgtcga aggagctgta ccatcattgt cagctacata ggtaaatttt
     2101 ggaacgatac tcgccggagg taccggaaaa tcatggtcac atgaaactat gccattaagc
     2161 atgattacga taagtaataa tttaagtata gatttcattc catacgaaat ttaagatgaa
     2221 ttaataagaa acacttcctg attcggaaca agttgaaatt agttaatgga aatacttatt
     2281 aaagtgaatc agcaatcaaa ctttgataga ttgctgattc actatatatt cctatcaatg
     2341 agcttttttc aaatgattag attacttgat gaatattcct cttgggctgt caagtccact
     2401 gaggaaggat tcttcaccac ttccatcgag attagctctt tttatttcac ctttatccct
     2461 gtctgcccaa tacaacttat tggcatcata gtcaattgct atgccatgga tacgagtttt
     2521 gccggtagat gaaattgtct ttgcccctgt gccatccaga ctagcctgca ttagcgcttg
     2581 ggcatttctt tcatcgtagt ataatttttc atttacttca tcaacaaaaa ttccatatcc
     2641 atatacgccg ggtatcaaaa ctgctacacc ggttccatct gcattcgcga tatacagatc
     2701 ttcattattt acctcataaa aatacagctt attggtctta ctgttgaagg caatacctct
     2761 catttgtccg tcagctacgt taatgaaaga tttctcaaca ttgcttccat ccagatttgc
     2821 cctggagata ttaccattat cgtccgccca gaatattttt cctcctttca aatcaatggc
     2881 aatactataa ggatcagtaa ttccggatgc tattttctcc aatcctgtac catctccatt
     2941 catacgccat atgatacctt catcagcatt ttcgaagtcg ctgtaataga ttttatcatt
     3001 agtggcatca tatgctaatc caactcctcc cttgccgacg atgtccaaaa acaactcagc
     3061 ctgagaacct tcggtaagag aaaccttact aattttattc ttgtccccca gctcgatgta
     3121 ataaagagtt ttcttgctta ccggcttggt tacagtgatt ttcgcactgg cagtggctgt
     3181 tcccccttct ccggtagcgg ttaagttgac cgtatattcg ccgacagcgg tgtaagcctt
     3241 tttagggctt ttatcggttg aagtcgttcc atctccaaat gaccaggtat atgaagtcgc
     3301 attttctgaa gtattggtaa atacaacttc gtcaccgacg aatacagtag tttgactggc
     3361 tgtaaaagaa gcaaccggtt ttggtttgac agggtcatcc tttttacatc cggtaaccgc
     3421 aagtactgca agtaaaaaca atggtaacaa attttttaat gatttcatgt gttaaaattt
     3481 taaatatgat ttaataaccc ggattttgct tcattccggg attggtattc gctaaaagtt
     3541 cactttgtgg aatcggaaaa agatactggt acttttgggt aacgttaggc agaagatcaa
     3601 ttgctctgtc tgtcctgacc aaatcaaacc aacggtggcc ttcaaatgcc agttcgactc
     3661 ttcgttctgc ttcgatggca agaagcagtt catctatgct attagcaacg gcctcctcta
     3721 accctgcacg acttcgtatg gtattaagat ctttccgaat atcattgata ttcccattta
     3781 aacgtgcatt ggcttcagcc cgtataagat acatttcggc caatctcaag acaattacat
     3841 tgtccgatcc tgtggcaagg tcatgatatt tgattgcata tgggatagtc ccggcaaagg
     3901 caatggaagc atccttgcgt ttgtcattgg cttcgtaggc actgacaatt ccggggtccg
     3961 gaccgacact gcctcttccg ttaagggtta acggaaaatt gtattctgca attctgtttc
     4021 tgtcaagaga ggtaaaatcg atctcaaaaa ttgactcctt tgattcatcg gcggcaaaga
     4081 tatctgagta attactcatc agggaataat tattgtcatc gattacctta ctggcataga
     4141 tgacggcatc ggcataatcc ttcttataca ggtacactct tgccaacaac gcatttgctg
     4201 ctccctgtga agcacggatc tttgatgcgg atgatttggg tagataagta gaagcaaaag
     4261 ttagatcctg gatgatagtg gcatagactt cttcaggact attacgtgct gcatttattt
     4321 cggaaaggcc tatggtaggc ttggttttga tgggaattcc cccgaaataa ttcaaaagat
     4381 tgaaatgatt cagagcccgc aaaaagtata actcggcaag tgctacattt ttttcatcct
     4441 gattcaaaaa actcatctca ggaattttag caataacatt gttagcaaca tttaaaccgt
     4501 catatgctac accccaaatt ccttcaatta ctccattttc aggcagaata tggttattgt
     4561 cgatatcgag gtaatcggca gtggtagcac tcgggggata aagcagattg tctgccgata
     4621 agtctgaaac ggtcagatag gctcttccgt agtaacttag accttgtaat gaattataag
     4681 cccccaaaat tccacggttt aaatcagctt tagtcttaaa tgcttcggat gcaggtatag
     4741 aattggttgg ttccacatcg agtacattgc aagatgagaa tgagtatacc agaactaata
     4801 aaatcaaaat ccttttcata ttaaaaaccg gcgtttaatc caaaggagaa acttctggcc
     4861 tgaggataag tgaaaaaatc tgttcccatt cgtgcattat catcacctac atagttgact
     4921 tcggggtcaa gacctgtata attagtaaaa gtaagcaggt tttgacccgt gaagtatatt
     4981 ctcaatgagc ttattttgcc tttcatgata ttctttgtat taaaattata accgaatgtc
     5041 aggttcttca atctgaggta ggatccgtct tcgaggaatc tggaagaaag tcgtttgttg
     5101 ctggcagctg caacggggtc gtttgtcgct ctgggaatgt cggtaatgtc acctacattc
     5161 ttccatcgtc tgttgattgc tgccaattga ttatcaccac cttgcaaaga ttccagataa
     5221 agcctcgacc cgttcataac gtcattcccc tgggaacctt gaaagaaaat ggaaagatca
     5281 aatctgccat atttgaaatt attggtgatg gcatatataa aatcaggatg aggatttcca
     5341 ataatggttc gatcatcgga agtaatatta ccatcaaaat tcttatccgc aaagactaca
     5401 tcaccggttg agggatctac tcccaaccat tcatagctat agaaaatacc gatgggttgt
     5461 ccaacttcga tccggttact tccacgaccc aaatcgtcta taggttgatt gttgtaaagt
     5521 ttggttattt tattcctgtt taaagagaaa ttgattctgg tgttccattc taatttccga
     5581 tctgcaaggt tttcggtagt taatcccaat tcaataccct tgttctccac ctcaccgata
     5641 ttttctgtga ttgtagaaaa accggaagat aatggcagcg gtctgcttaa caacaaatcc
     5701 ttggtgaatt tacgatataa atcaatattc agataaatcc tgtctttaaa taagcctatg
     5761 tccaaacctg cattcacctg agtggttgtt tcccatttca gatcaggatt tggaatattg
     5821 ctgggataga cagcaggttt gcctccataa gtagtgttgc catataattc ggcatagaga
     5881 aattgcggga tatcatcatt gcctgttagt ccgaagcttc cacggacctt caactcagaa
     5941 attacattgg acttgaagaa gtcttcctca ccaagtctcc aggcagctga aacagccgga
     6001 aaaaagccat tacgattgtt ctcgctgaat ctggaagatc catccatacg acctgtcagt
     6061 gatacaagat atttatcctt caggttgtaa ttgattcgtc caaacaatga tcttatacct
     6121 tcattagacg caccggagct tgcagatgta attgtagagg cgctgttgat gtactgaaga
     6181 cttgcatctg cataatcctg acctctgata taggaactag ctacattctt tctttcgaat
     6241 gaataacctg ccagtacttc aaacctgtta tcctgtaaat caaaactgta tttcaaagta
     6301 ttattggaaa ccagattacc aacattagta tatgtttcga atcccagccc gttgtatttt
     6361 ctaccctgaa cagtattata ttcaaaggca tgctccctga aattcataaa gtcaataccc
     6421 catttagtag aaaaggtaag ccctttccag attttccagt ccagatatat attccccaat
     6481 ccacggtaag aaaagttttc attggttgcc tcattggcta tcgaaaccgg attagaataa
     6541 gggccatcct gattataaga accatcgctg ttatatacgg ggaatactgc gggtgttgaa
     6601 atgccattgg gcaaaacacc atgcagggat tggtctcctt ctattctgtt agtcttggca
     6661 taggacaagc cgatacttgt accaatggta acagaattgt tgattttatg atccagattg
     6721 acccttccgt tcatacgcct gtaatcactt cctatcaggg tacctgtttg attgaataag
     6781 tttccactga tgtaaaatcg tgttttttca tcaccgcctg atgttgataa ttcataattg
     6841 gagattggag cggtacggaa aatgtagtcc tgccattttg tatctatagt gttgtcattg
     6901 atttgctcag gggtataaac ttctgttccg aaatagtcat tcctgtacag tttccaatcg
     6961 tgggcattca gcatttccag tttcctccag ggttgttgcc aaccataaga ggcagataaa
     7021 ttaatggtag acttcttact gcttcctcgt tttgttgtga tcagaacaac tccgttggag
     7081 gctctggcac catagatggt tgcagccgct gcatctttga ggatggtgat tgattcgata
     7141 tcgccgggat tcaggtctga cagttggttt actccctggc cttcatagcc tacctgcgca
     7201 aaatcacctg ttgtgacagg gataccgtca atgacataca aaggttggga actgccgctg
     7261 atcgaactta cacctcttat ccttacggac atgcctccac cgggtgtgcc ggaattctgt
     7321 actacctgaa caccggaggc ctggccttgc aacacaccgt caatggtagg tacgggaaga
     7381 tctttgatag catctgaatt gacaatacct actgatgaag taagagtctt cttttgttca
     7441 accccataac ctactacaac cacttccttc aaggccttca cgtcttcctg cattaccaca
     7501 tcaatcattt ttctgccatc tatattgatc ttttgtgtct ggtatcctac ataggaaaat
     7561 tccaaagcat ttgctgtatt cactcgtaaa gagtaatttc catcaatatc ggttacggta
     7621 ccattattct ctcctattgc tagaatagta acaccaacca ggccctggcc ggaagcatca
     7681 cgtactgttc cgcttatttg tccatgcagg ttaccgaata agaaaaggag cagtaaaacc
     7741 aatccttgtt taagtacata tctggtcata caactgaatt tgtaaataaa ttagcaatca
     7801 ttatttctat catttgcaaa atgaaaggaa aaaactttca cctacaaaat tataactatc
     7861 gcccaattat aaaaattaat ttccttaact taaaaataat taaccatgca cacctcactg
     7921 tttggtttta tctcatttta atgtaacctt ggttggctct atattaggga gtgccaaggt
     7981 agaaatttcg actttaattc aatgtcccat ccaatatagt cattatggcc tttaataaat
     8041 gagcgttttt ataaaccata tccaacttaa atatatataa tattttaata tctttgtagc
     8101 aaatatatat atatacagag ttaaggaacc ttttgccaat gaaatattcg tctcaattaa
     8161 tagggaaagc tgtggaatca ttcagttcat tacccggtat cggtaagaag actgcactac
     8221 gtctttctct ccatctgtta agcaaggatg ccatgtgggt acaggctttt tcggaatctt
     8281 tcataaaact tcgaaatgat ttgaaggagt gcgacgaatg ccacaatctg ggggatgagg
     8341 ttctgtgttc gatatgtacc gacaagagaa gagacaatgg aatcctgtgc attgttgaga
     8401 ccatccgcga tcttatggca atagaagaaa ccggtcattt tcagggattt tatcacgtag
     8461 ttggtggtct catttccccg attgacggaa ttggtcctga tcaactgaac attgaagata
     8521 ttgaacagcg aataatcaat aagaacatta gggaggtggt aatggctata agccctaata
     8581 ttgaaggtga gacaacgatg ttttatatag ctaagaggtt gcaacatctg cctattcgga
     8641 tttcgactat ttccagagga ataagtttcg gaggagagtt ggaatacgcc gatgaagtaa
     8701 cactcggaag gtcgatcatg gccaggatac cttatatgca gtttgaacct tctactgctg
     8761 agcatggtga ccgttgaaga attgttggcc gttagatatc tgcagattgc cttcttttta
     8821 attttttatt gggcaaaaaa atcggtactt aattgaccat ttacagaaaa tttacataat
     8881 ttacacgctt taatttggct tcggaaagcg aataagtttg cgttgagtat tgtttttctt
     8941 caggattgtg atatttataa tattgacaga ctgcctaaaa aattgtgatg tatggcggaa
     9001 tattttgaga gattgatgca tggatttgag ttgcctttga aaagccctgt acttgttttt
     9061 tcattgatac ttctgattat tcttctttct ccgatattga tg
//
LOCUS       DAOQQN010000080         9099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2878_length_9099_cov_4.862450, whole genome shotgun sequence.
ACCESSION   DAOQQN010000080 DAOQQN010000000
VERSION     DAOQQN010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9099
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2878_length_9099_cov_4.862450"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PLG24_04445"
     CDS             complement(<1..285)
                     /locus_tag="PLG24_04445"
                     /inference="COORDINATES: protein motif:HMM:NF017373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24169.1"
                     /translation="MENVDFKMFDNFIQSVLSGLPKFLGAIGLLILGYVIARVVRAAV
                     RTFLAKIKVDKLADKLEEIDLVAKSKIKIVPSKIIASLIYYLLLIIFVVAV"
     gene            complement(329..532)
                     /locus_tag="PLG24_04450"
     CDS             complement(329..532)
                     /locus_tag="PLG24_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24170.1"
                     /translation="MYDFNVFDELEHKQIEENGGVPEQVHENLKGSIRLFSLIGNCME
                     LFIPKFFGTFNAFLGDDRLEEKE"
     gene            complement(557..1126)
                     /locus_tag="PLG24_04455"
     CDS             complement(557..1126)
                     /locus_tag="PLG24_04455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ24171.1"
                     /translation="MIAVDYIKWTDNTLVEEYLLTQHMAYFDELYKRYSNKVYFKCYS
                     LLGDYTQAEDATQEIFLKVVLSIAGFNRKSKFSTWLYSITYNFCIDELRRLKKNKMVF
                     DVEGDIHGELPDDEEDNLTSEANLVALQATLDVIDEADRAILIMKYQDNMMVKEIADI
                     LSLSESAVKMRLKRAKEKFKKLFLDILRQ"
     gene            complement(1447..2835)
                     /locus_tag="PLG24_04460"
     CDS             complement(1447..2835)
                     /locus_tag="PLG24_04460"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HMZ24172.1"
                     /translation="MSKRIFFVLMLLTFFNSCIFGQLSEDKRMIRQLHELSVTKGKAY
                     DWLEFLCKDIGHRLSGSPQLAAATNYTYYLMKELGFDSVWLQSCKVPHWERGPKEIVR
                     VTGSVEGQFDLSALALGNTWGTGPNGVSGEVIAVYGLEQLSKMPDSLVKGKIVFFNRP
                     FAEAPLNTFESYGMTVDQRGGGPALAAKKGAIAALVRSVTSSNDDIPHTGGSWFGEEG
                     PFIAAYAIGCQSADRLAHALKLGKVVAYCQSDSRMLPDADGWNVIGEIRGSKYPEEII
                     VVGGHLDSWDVGEGAHDDGAGTVQSIEVLQLLRLAGYKPQRTIRAILFTNEENGARGG
                     KAYKTFTDKTNEKQILAIESDAGGHSPQGFTWESTDKYEGKYRDRLMQWKNILDEYGY
                     RFIFGYSGVDIEPLKPHHGLLMGLLPDSQRYFDYHHSAKDTFDKVNRRELELGAAAMA
                     SIVYLADRYGIR"
     gene            3435..4760
                     /locus_tag="PLG24_04465"
     CDS             3435..4760
                     /locus_tag="PLG24_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ24173.1"
                     /translation="MSHFETKALRAGYNPANSAGSSSVPIFQTVSYVFEDTDYAAGVF
                     NLSRPGYIYTRLNNPTNDILEQRIAALEGGIGAVVTSSGTSAIATTLLTLLRSGDHIV
                     ASESLYGGTFNLLNVTLPRFGITSEFVDPSAPDNFSRAIRENTRAIFIESLGNPKLDV
                     LDIKAIAAVAKAHRIPLIVDNTVATPYLLRPLEHGANIVIHSLTKYAAGNGTTLAGAI
                     IDGGTFDWSNGKYPEFTEPSPGYHGLIYHDVLGASAFIAKIRIEGLRDLGAALSPFNA
                     FQVIQGLETLPVRVSKHSENALNLARWLEEQDEVAWVNYPGLPSSNYYDLAKQYLPNG
                     QSGLLTIGLKGGYEAAKIVANETKLFSLLANIGDTKSLIIHPASTTHQQLSEEDQGAS
                     GVTPDLLRISVGLENIEDLKADLLQAFQAIYSNSHEKVEEYINQELSLT"
     gene            4714..5670
                     /locus_tag="PLG24_04470"
     CDS             4714..5670
                     /locus_tag="PLG24_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMZ24174.1"
                     /translation="MKKLKNILIRNFPLPDGSKESFKVSYQIFGQELHTAPIILINHA
                     LTGNSSVAGEDGWWKEVVGDSKAIDLRRYTVIAFGIPGNGYNHVAPIERYTLLTTKIV
                     GNLFWKALDSLGVVQLYAILGGSLGGSVAWEMLFQRPDSIQKLIPIACTLKSSDWLIG
                     NAHVQESILSNSQNPLEDARKHAMLLYRTPNSFRAKFKGRQSESGFAVEHWLIHHAEQ
                     LKSRYTLPSYRLMNHLLKTIGENRAKDEVEKIFRNCKTEIHTIAVDSDYMFTREEQIE
                     TYKIIRKYKKNASYGEIKSLHGHDAFLIEFQQLNKLLISILS"
     gene            5667..6923
                     /locus_tag="PLG24_04475"
     CDS             5667..6923
                     /locus_tag="PLG24_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24175.1"
                     /translation="MKVINLNQSSSIQQEHRQVTQIKNFGTTSSINISSDARKPLRFD
                     SARQADNRHCQVIKTINIAIFGTGKVGGTLIDQILASGINIEKSKSIRLNIFAVGNSQ
                     KLHLDKSGISENWKFYLNKYGKHYDIWDIITYAQVHQLDNLIAVDNTASEEFTENYHT
                     LVRNGFDLVSSNKIGNSGSYEFYRSLRQILRKNEREFLYEANVGAGLPLIDTIRMLHI
                     SGDKIHRIRGVFSGSLSYIFNTFSSRDIPFHQVLHEAASKGLTEPDPREDLSGNDVCR
                     KLLILARELGLSQEFESIDNHNLIPEELGKLDLDSFQKNSHELDRHFEYLKQNVKPGH
                     VLRHIGELSINRQSCEVLLKAGLSEIPEYSALGQLTGSDSLFEIYTEGYGLNPIIILG
                     AGAGPAVTARGVFSDILRLAGRVMKN"
     assembly_gap    7531..7630
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7925..>9099)
                     /gene="tyrS"
                     /locus_tag="PLG24_04480"
     CDS             complement(7925..>9099)
                     /gene="tyrS"
                     /locus_tag="PLG24_04480"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HMZ24176.1"
                     /translation="IGFDPTAPSLTIGNMVQVMLLSHFQRTGNKPIVLMGGATGMIGD
                     PSFKDAERTLKTIEEIQVNIDRQMKQFRKLLDFDGPNAAVFVNNYDFYKDMNVLTFLR
                     DVGKTLTVNYMASKESVKKRMETGISYTEFSYQLLQGYDYQVLYDRYNCRVQMGGSDQ
                     WGNITAGTEFIRRNVGGKAYAATTPLLTKSDGTKFGKSESGNIWLDPGMTNAYQFYQF
                     WINCSDEDMPKLLRYFSLKNKKEIEELDLMLVDNPNEVKRILAEEITTRVHSAEDLDA
                     VKKVSSLLFGKGFDEQAVKSMGTNTIELLKGELEVRSGSADQWSQGASVIDLLTELNI
                     FTSKGEAKRAIQGQAVQINRVRITDPAAISEPEQIFGNGYVFVENGKKQKVLIELA"
BASE COUNT         2796 a         1934 c         1730 g         2539 t
ORIGIN      
        1 cactgcaact acaaagatga tcaagagcaa ataatagatc agactagcaa ttattttact
       61 aggtacaatc ttaattttac ttttagctac caggtctatc tcttcgagtt tgtccgcgag
      121 cttatccacc tttattttgg caaggaaagt tcttactgcg gccctgacga cccgggctat
      181 cacatagcca aggatcagca ggcctatagc acccaggaat tttggtaaac cgctaagaac
      241 ggattggata aaattgtcga acattttaaa atcaacgttt tccatggtgt atgattctta
      301 attatttatg attgtgtgtg aagtatattc attctttttc ttccaaacga tcgtcaccca
      361 aaaatgcatt aaacgtacca aaaaacttag gtatgaacag ctccatacag ttgccaatca
      421 agctaaatag acggatgctg cctttcaagt tttcatgcac ttgttccggc actccaccat
      481 tttcttcaat ctgcttgtgt tctaattcat caaatacatt aaagtcgtac ataatgtttc
      541 gtttaaattc cagtacttat tgccggagga tatctaaaaa caactttttg aatttttcct
      601 ttgctctttt aagtctcatc tttactgcgc tctctgacag gctgagaata tccgcaatct
      661 ctttgaccat catattatcc tgatatttca taatcagaat cgcccgatca gcttcatcta
      721 ttacatctaa ggtcgcctgc aatgcaacca gattggcttc actggtaaga ttatcctcct
      781 catcatcagg caattcacca tgtatatcac cctcaacatc aaataccatc ttattcttct
      841 taagccttct caattcatcg atgcagaaat tataagtgat tgaatacaac caggtactga
      901 acttagattt tctattgaag ccggcaatgc taagaacaac cttaaggaag atctcctgag
      961 tagcgtcttc agcctgggta tagtcgccaa gcaaggagta acacttaaaa taaactttat
     1021 tggaatatcg cttatacagc tcatcgaaat aagccatatg ctgagtcagc agatattcct
     1081 ctacaagtgt attgtcagtc cactttatat aatctacagc tatcaaatcc gtatatccag
     1141 gtttgaaatt tgctgcaaag atgtaaaata atcaggacaa caataaggag gggctgtaaa
     1201 tttatcaaaa tcctcctttg caatatcatt ccattccaaa tgcaacaaca atgttaaata
     1261 ttagcagaaa cctattttct gacattctga taaagattag acgagaaata tcacaatcgt
     1321 cacccgatca ttcaggaaat tttaaataaa acacttagta atttacttca gcaagcgaat
     1381 caatccaaaa ctttgttttg agccaacatc accctagata acaggtccaa ctaaaagtta
     1441 gttttctcac cgaattccat accgatcggc cagatacacg atgctagcca tggcagcagc
     1501 ccctagctcc aattcacgac ggttgacctt gtcaaatgtg tcttttgctg agtggtggta
     1561 atcaaagtaa cgctgagaat ccggcaacag gcccatcagc aagccatgat gaggcttcaa
     1621 tggttcgata tccacgccgg aataaccgaa aataaaacga tacccatact catcaaggat
     1681 attcttccat tgcattagcc tgtcccgata tttcccttca tacttatctg tggactccca
     1741 ggtaaaacct tggggtgaat gtccgcctgc atcggattcg atcgcgagaa tttgcttttc
     1801 atttgtctta tcggtaaatg ttttgtaagc tttaccccct ctagctccat tctcttcatt
     1861 cgtaaaaaga atggcgcgaa ttgtccgctg gggcttgtat ccggccaatc tcagcaattg
     1921 caggacttca atagactgga cagtacctgc accatcatca tgagcacctt ctccaacgtc
     1981 ccaactatcc aggtgtccac ctacaacgat gatttcttcc gggtatttac tacctctgat
     2041 ctcgccgatt acattccagc cgtctgcatc cggaagcatc ctgctatctg attgacaata
     2101 ggcaacgact ttgccgagct tcaatgcatg agccaggcga tccgctgatt gacagccgat
     2161 agcataggca gcgatgaaag gtccctcctc gccaaaccag ctacccccgg tatgtggaat
     2221 atcatcattg gaactcgtca cactccgcac caatgcggca atggcgccct tttttgctgc
     2281 aagtgctgga ccgccccctc gctgatctac tgtcatgcca taggattcaa aggtattgag
     2341 tggcgcttct gcaaagggtc ggttaaagaa tacgatttta cctttaacca atgaatcagg
     2401 catctttgaa agctgctcaa gcccatacac agctattact tctccactaa ccccattcgg
     2461 accggtgccc caagtgttgc ccaaagccag cgctgaaagg tcgaattgtc cttcaacaga
     2521 accagtgacc cttacgatct ccttagggcc acgctcccag tgtggcactt tacaagattg
     2581 aagccaaaca gaatcaaagc caagttcttt catgagatag taggtatagt tggtcgcagc
     2641 ggctaattgc gggctaccac ttagcctgtg accaatatcc ttacagagga actcaagcca
     2701 gtcatatgct ttgcctttgg taactgacag ctcatgcaat tggcgaatca tcctcttatc
     2761 ttctgaaagt tgaccaaaaa tgcatgaatt gaaaaaagtg agaagcataa gaacgaaaaa
     2821 aatcctttta ctcataccga ataattattt acttagttat atatacaaaa gtaataaaac
     2881 aggaatccaa acaaataatt attgaaacca ttcatatgac aaaatgaata ctgaacattt
     2941 ttatcttaac tttatattat tgattatatg ttatatacat attatataat aaaatattta
     3001 aacaaacatt acctcatttt aaaaaataat ttctcatctt tgctgcacga aaaagaaatc
     3061 aatgaatcag attaataaca atatgcaggc catgatgatg tgtataggta cacgaggctt
     3121 gttatttgta taatctgaat ataaaaagat aaatatataa tgagcctctg cagatgcagg
     3181 ggcttttttt ttgacattat gaaaaaaata aaattcgaaa gatatcggct gatagtcact
     3241 acatctagtg accaaggagc aatgtgttgt tgtatgatgt gcatgatgat gcgccacaaa
     3301 gcccgttgcc ttaagtcacg agattagaga tccaaagaaa atctgtaaga agtcctttcg
     3361 gcaataaccg gaaggacttt tttttaacct taaattatca ttgtaaacat tgtaaacatt
     3421 taaaattttt aaaaatgagt cattttgaaa ccaaagcatt aagagccggt tataatccgg
     3481 caaattcagc aggcagtagt tctgttccaa ttttccagac tgtatcgtat gtatttgaag
     3541 atacggacta tgcggcaggc gtctttaacc tttcaagacc gggatacata tacacccgtc
     3601 taaacaaccc gaccaatgac atcctggaac aaaggattgc agctttggag ggtggcatag
     3661 gcgctgttgt gacttcatcg ggcacatctg ccatcgccac taccctgctt acgctactca
     3721 gatcaggcga tcatatcgta gcttccgaaa gtctatatgg aggcactttc aatctactga
     3781 atgttactct acccaggttt ggcattacaa gcgaattcgt cgacccttca gctcctgata
     3841 atttcagcag ggcaatccgt gaaaatacca gggctatctt cattgagagt cttggaaatc
     3901 ccaagcttga tgtgttggac atcaaagcta tagcagcagt agctaaggct catagaatac
     3961 cgttaattgt agacaacact gtagcaactc cttacttact aaggccatta gagcatggtg
     4021 ccaatatcgt cattcactca ctcacaaaat atgcggcggg caatggaacc actttagcag
     4081 gggccattat tgatggcgga accttcgatt ggagtaatgg taaatatcca gaatttacgg
     4141 agccatcacc aggataccac ggtttgatat atcatgatgt attaggcgct tcagctttca
     4201 tcgcaaaaat taggattgag ggtctcagag accttggtgc agccttaagt ccattcaacg
     4261 ctttccaggt tatccaaggg ctggaaacct tgcctgtcag ggtcagcaaa catagcgaaa
     4321 acgcacttaa tctcgctcga tggcttgaag aacaggacga agtcgcatgg gtcaactatc
     4381 ctggtctgcc atcaagtaac tactatgatc tggcaaaaca gtatctacct aatggtcaga
     4441 gcggtttgct aacaattggt ctgaagggtg gttatgaagc ggctaaaatc gttgccaacg
     4501 aaacaaaatt attctcactt cttgccaata taggcgacac aaaatccctt attatccatc
     4561 cggcttcaac aacacatcag cagttgtcgg aagaagatca gggagcatcc ggtgtaactc
     4621 ctgatctttt acgaatatct gttggactgg aaaacataga ggacctgaaa gcagatttat
     4681 tgcaagcatt tcaagccatt tattccaata gtcatgaaaa agttgaagaa tatattaatc
     4741 aggaactttc ccttacctga tggtagcaaa gaaagtttta aagtttccta tcaaattttt
     4801 gggcaagaac tccatactgc tccaattatc ctaatcaatc atgcactcac cggcaacagt
     4861 tcagttgccg gtgaggacgg ttggtggaaa gaggtggttg gagactccaa ggcgatcgat
     4921 ctaaggcgat atactgtaat agcatttggt attcccggca atgggtacaa ccatgttgcg
     4981 cctatcgaac gatacacatt actgacaacc aagattgtcg ggaacctgtt ctggaaagcg
     5041 ttggacagtc ttggtgtagt acaactttac gccatcctgg gagggagcct aggcgggagt
     5101 gttgcatggg agatgttatt tcaaaggcct gattccatcc aaaagctgat accgatcgca
     5161 tgtacattga aaagttccga ttggcttatt ggcaatgcac atgtacagga aagcattttg
     5221 agcaattcac aaaatccact tgaagacgca agaaaacatg cgatgctttt gtacagaact
     5281 cctaattcat tcagggcaaa attcaaaggc aggcaaagtg aatccggatt tgctgtagag
     5341 cattggctca tacaccatgc tgaacaacta aaatcaagat atacgctccc atcctaccgt
     5401 ctgatgaacc atttattgaa aacaatagga gaaaaccgtg caaaggatga agttgagaag
     5461 atttttagga attgtaaaac ggaaatccat acaatcgcag ttgattctga ttatatgttt
     5521 acccgtgaag aacaaataga aacgtataaa attattcgta aatacaagaa aaacgccagt
     5581 tatggagaga taaagtccct ccatggccat gacgcattct tgatagaatt tcaacaattg
     5641 aacaagcttt taatatcaat actctcatga aagtaattaa tctgaaccaa tcttcttcca
     5701 tacaacaaga acacagacaa gtaactcaga tcaaaaattt tggaacaaca tcatcaataa
     5761 acatatcttc ggatgcgaga aagcctttga ggttcgactc agcaaggcag gcagacaaca
     5821 ggcattgtca ggtaattaaa acgataaata ttgccatctt cggcaccggc aaggtagggg
     5881 gtactttaat cgatcaaata ctggcatccg gcataaatat tgagaagagt aaaagtatta
     5941 ggttaaacat ttttgctgtg gggaattctc aaaaattaca tctggacaaa tcaggtatca
     6001 gtgaaaattg gaaattttat ctcaataagt atggaaagca ctacgacatc tgggatataa
     6061 tcacgtatgc tcaggtccat caactggata atctgatcgc agtagataat actgcctctg
     6121 aagaattcac tgaaaattat catactttag tcagaaatgg tttcgatctt gtctcttcca
     6181 acaagattgg caattcagga tcctatgaat tttatcgcag tttaaggcag attctccgca
     6241 agaatgagag ggaattcctg tacgaagcca atgtaggagc cgggctgcca ttgattgaca
     6301 ccatcagaat gcttcatatc tccggagaca aaatccaccg gatacgtggt gtgttctcgg
     6361 gtagcctcag ttatatattc aatacgtttt catcaaggga tatcccattt catcaggttt
     6421 tacatgaagc tgcttcaaaa ggcctcactg aacctgatcc ccgggaagac ctgtcaggca
     6481 atgatgtctg ccgaaaattg ctcatcctgg caagggaatt gggcctgagc caggaattcg
     6541 agtccatcga caatcacaat cttattcctg aagaattagg caaattggat ctggatagtt
     6601 tccaaaaaaa cagccatgag ctggacaggc actttgaata cctcaagcag aatgtcaagc
     6661 cggggcatgt cctccgccat ataggagagt tatccatcaa ccgccaaagt tgcgaagtac
     6721 tgctcaaagc gggcttatct gaaattccgg agtattcggc attggggcaa ctaaccggtt
     6781 ctgacagctt gtttgaaatt tacacagaag gctatggatt gaatcccata atcatcctgg
     6841 gtgcaggagc cggacctgca gttactgcgc gtggtgtatt cagtgacata ttacgtttgg
     6901 caggcagggt catgaagaac taggtattat gaagtattgg aatggagaat ggcaatgtgg
     6961 gcattaaatg aagggaaaat ggagatatgg ttggggaaaa tcgaagtatg tacatgatta
     7021 ataattgaaa actggcgagc tacaaggggt ctggtaaatc tatcccaaaa aaaataacca
     7081 tttggaccaa atttttcaga ggtataaact aagaatacca tttcttggtg gaatgctggc
     7141 tattaatcat ctacctactt caggtgacag ctcattcaag atacaaattt aattacaatg
     7201 ggccggaaaa atcgaagtat gtacatgatt aataattgca aactggcgga ctacaaaggt
     7261 tctgataaat ctatccaaaa aaaataacca tttggaccaa atttttcaga ggtataaact
     7321 aaaaatacct ttccatggtg gaaagctggc tattaatcat ctacctactt caggtgacag
     7381 ctcattcatg ataaaaatgt gattgcaatg ggccggaaaa atcgaagtat gtacatgatt
     7441 aataattgca aactggcgaa ctacaagggg gctggtaaat ctatccaaaa aaaataacca
     7501 tttggaccaa atttttcaga ggtataaact nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn taaaaatgtg attgcaatgg gccggaaaaa tcgaagtatg tacatgatta
     7681 ataatgtcaa actggcgaac tacaaaggtt ctgataaatc tatccaaaaa aaataaccag
     7741 ttggaccaaa tttttcagag gtataaacta agaataccct tacttggtgg aaagctggct
     7801 attaatcatc tacctacttc aggtggcagc atatttaaca ctcacttcca agtctgactc
     7861 caacccagta atgcaaaaga taaataaaaa tgtcttgcga cgtccaatgt agtctgctca
     7921 ggttttacgc caattcaatg agtacttttt gtttctttcc attctcgaca aagacatatc
     7981 cattgccaaa tatttgttcc ggttctgaaa ttgcagctgg atcggtgatc cttacccggt
     8041 tgatctgaac agcttgcccc tggattgctc tttttgcttc accttttgaa gtgaaaatat
     8101 tcagttccgt gagtagatct atgactgatg ccccttggct ccattgatca gcagacccgc
     8161 tcctaacctc caactcccct tttaatagct cgatagtgtt agtgcccatt gatttgacag
     8221 cctgctcatc aaatccctta ccgaagagta aagaagaaac ttttttaaca gcgtcaaggt
     8281 cttcagcact gtgtactcgg gttgtaattt cttcggcaag gatgcgtttt acctcattcg
     8341 gattgtcaac taacatcaaa tccaattctt ctatctcctt tttattcttt aatgaaaaat
     8401 aacgcagcaa cttaggcatg tcttcatcac tgcaattaat ccaaaactgg taaaattggt
     8461 atgcattagt catcccaggg tctagccata tattgccact ttccgacttt ccaaattttg
     8521 taccgtccga cttggtcaat aacggagtgg tagcggcata tgcctttcct ccaacatttc
     8581 ttcttataaa ttcggtacct gctgtaatat taccccattg atccgagccc cccatctgca
     8641 cacgacagtt atatctgtca tatagtactt gataatcata tccctgaagt aactgataac
     8701 taaattctgt gtaagaaata ccggtctcca tacgtttctt aactgattct ttacttgcca
     8761 tataattgac ggtaagtgtt ttccctacat cacgaagaaa cgtcagtacg ttcatgtctt
     8821 tgtagaaatc gtaattattg acgaatacag ctgcatttgg tccatcaaag tccagtaatt
     8881 ttctgaattg cttcatttga cgatcgatat tgacctgtat ctcctcaatt gtcttgagcg
     8941 ttcgttctgc atccttaaag gaagggtctc caatcatacc ggtcgctcca cccatcagaa
     9001 caatgggttt attaccggtt cgttggaaat gagacaacaa cataacctga accatattcc
     9061 caattgtcag ggatggtgca gttggatcaa acccaatat
//
LOCUS       DAOQQN010000081         9080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2887_length_9080_cov_4.813740, whole genome shotgun sequence.
ACCESSION   DAOQQN010000081 DAOQQN010000000
VERSION     DAOQQN010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9080
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2887_length_9080_cov_4.813740"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2528
                     /locus_tag="PLG24_04485"
     CDS             <1..2528
                     /locus_tag="PLG24_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ24177.1"
                     /translation="ECADITFTDQIVNGACAGTYTIFRTYTAKDCCGNMSTYTQKINV
                     VDDTPPQIIPIIRPLSTLKSGDILTTYCGADEFPTWLNQPVNSLISAVDACSSNPKLS
                     LNISEENQDACWLYGYSKQYTVSLTATDACGNQSELKFYIQVKDTTAPQVMYLNEFIC
                     EDDNAVPVVFDNCSSLTYETNDIPVTGECEGSQNFIRVWTISDACQNTIYASQYVVKN
                     DHKAPVIHMINPAFADHLSGDIVSLDCNNWQDVSAAEALTWVEAEDKCDYITLDFNSR
                     LINGNCLSDGFKSMYEYTWTAADYCGNQSTFVLFVQLTDNTPPVFNTSLTSINVDCEN
                     SIPVVKATDNCSDVTLTVTRTKFELGCPNNFTLDEVYTATDACGNVSTFSRVVTVMDT
                     VGPVIYVPDAICETDLGTQNVVAFDYCTGKPSHVSANVDHNIVNCNGSSYYTITYSAT
                     DYCGNTSTKVQKVIVEDNTPPVLSFSYDMQNQYNIVDNTIYAGCGNFEQIFKLIDEYD
                     AVVVSDECGTIITPTYSESEGETKCEGDYITKEHFFKWTATDACGNTSNLDLKVVLHA
                     LESYDFSFVPADTTVYCNEVVALPTVLPELNCTEVSLSSSINLGQASPDGSYIEERTF
                     TYTNLCGEETSYTQIINHTFNSDLACNITIPTEVYCNSSNNLFTVVVTGGQAPYTYDW
                     EILNGWCHILGGQHTPTVQISISFKTLYLKVKVTDARGCSTECYVEVDCSLDNPGINV
                     VDPNVIDNSSTIGQLNVINDYTLRPNPTSAQVYLDFESKLAEKVTINVTDNFGKLVYT
                     KKVTTVKGFNTELLETNHLPVGLYQVSLISSDKLKTLQLIKVK"
     gene            complement(2824..3438)
                     /locus_tag="PLG24_04490"
     CDS             complement(2824..3438)
                     /locus_tag="PLG24_04490"
                     /inference="COORDINATES: protein motif:HMM:NF016983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HMZ24178.1"
                     /translation="MNMNENQVIEKQADSIIRNSIVFASGAGLIPVPVVDLMAVTAVQ
                     IEMIRRLSNLYKVDFKETEGKAAILALTSGGVARLGGRMIAKMIPVIGSIVGGIAASA
                     FSGAATYAVGEVFKRHFSEGGTFLDFDWKSFKHFYNEKFEKGKEYTAKIKKEEADNQP
                     QAQSEATNIYLDALQRLYDMKSTGAINEDDYEKLKADIMNKMGV"
     gene            3617..4570
                     /locus_tag="PLG24_04495"
     CDS             3617..4570
                     /locus_tag="PLG24_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HMZ24179.1"
                     /translation="MIRYYAKQQGTLVELPDYAVHCWINIEPPFSHDELEDLSTRLNI
                     PLDFFTDPLDIDERSRYEKEGPARLIVMNSPILNETDRESESIYITIPIGIIFSPESV
                     ITICAHENPVLELFLEDKIKNFNPANIQHFILQILEQNVYRFISCLKKLNLKRNLLEQ
                     ELYDSSRNSELWQMLRIEKSLVYFVNSLKANELLKVKMKRIDFLGLGLNEELEEIYED
                     LMIDNSQALEMANIYTVITGSTMEAFASIISNNMNVDIQRLTFVTIMLMVPTIITGFY
                     GMNVKLPFENYKYASFFIIIFSVLVGAGLIYFLRQKRFRSS"
     gene            4822..5868
                     /gene="lpxD"
                     /locus_tag="PLG24_04500"
     CDS             4822..5868
                     /gene="lpxD"
                     /locus_tag="PLG24_04500"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HMZ24180.1"
                     /translation="MEITAGFITDFLGGTIIGDRNVVINGASTIEGGRPGTISFLSNL
                     KYEEYIYNSEASAILVDNSFEPSYPVKAALIKVENVYESLARLLDKFNVTTEIERHIS
                     PKAHIDDKAVLGNNVGVGVFSVIEENVRIGDNVEIENQVTIGQGVTIGQGTIIKSGVK
                     ILEGCVIGANVIIHSNTVIGSDGFGFAPTGEGNYVKIRQLGNVVIEDDVEIGSNCSID
                     RATFGSTIIRKGAKLDNLIQIAHNVEIGSNTVIAAQTGIAGSARIGEGCKIGGQVGIV
                     GHIQIADGTQIQAQSGVQSAVKNKNSKLFGSPALDYNNYLRSYAIFKNLPALAEKVEK
                     IARPHLGKANEPDA"
     gene            5949..7346
                     /locus_tag="PLG24_04505"
     CDS             5949..7346
                     /locus_tag="PLG24_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HMZ24181.1"
                     /translation="MLQKTIKDKVSIKGIGLHTGKTVEMTFIPAAENHGIKFQRIDTP
                     EQVIIPADILRVVSTNRGTAISSGEITIYTIEHALAALTGLGIDNVLIEINGPEVPIL
                     DGSAKPFVDAIEQTGIVEQNEEKDYFIITEPISYKDETTGSEMIALPGDDFEVTTMID
                     FASPVLGEQFAKLDHISQFKEEISACRTFVFLHEVEKLLNQGLIKGGDLDNAIVIVDR
                     MMNQQELDDLAKKLGKPSVKIEKEGVLNTLKLHFSNEPARHKLLDVIGDITLLGRPIK
                     GKIVATKPGHSVNVEFTKLLRKAANEQKKLKGKPIYDPDKDAVMDTTQIMGMLPHRHP
                     FLMVDKVIEINENYVVGVKNISFTEACFQGHFPGNPVFPGVLQIEAMAQTGGILCLSL
                     MPDPENWDTYFLKITNAKFKQKVVPGDTMVIKMELLEPIRRGICHMYGIAYVGNKIVS
                     EADLTAQIVNRTTIK"
     gene            7346..8119
                     /gene="lpxA"
                     /locus_tag="PLG24_04510"
     CDS             7346..8119
                     /gene="lpxA"
                     /locus_tag="PLG24_04510"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HMZ24182.1"
                     /translation="MTPKFSDIHPNAQIGIGTQINNFVTIEDDVQIGENCWIGSGAVI
                     MKGARLGNNVRIFPGAIIAGIPQDLKFQGEESLVEIGNNTTIREYVTVNRGTAAAGTT
                     IIGENCLIQAYCHVAHDCKIGNNCIMSNNTALAGHVVLEDYAIIGGMSAVHQFSRIGC
                     HTMIGGYSKVRTDVPPFIKAARDPLSYAGVNSIGMKRRGFTQDRVHHIQDIYRILFVH
                     GTNFHKSIEQIENNIPNTEDKTYILNFLKSSTRGLIKGI"
     gene            8135..8782
                     /locus_tag="PLG24_04515"
     CDS             8135..8782
                     /locus_tag="PLG24_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ24183.1"
                     /translation="MIPKVEVITCKEIGKRYSYEWVLRDFDFQFKKGECYAIRGPNGS
                     GKSTLLKILSGYSTPSKGKICYSSGNTDIPTSDIYRHIAFSAPYIELIDILTVDELLD
                     MHHGLRPFINMDTVRETIKNLPFKNMFRKKVEELSSGMKQRLKLILSIMSQSSVILLD
                     EPGSNLDDDGKQWFEELLKPRIKDHIVIIASNEESDLRLAQHLIKITDYKKAKQS"
     gene            complement(8860..>9080)
                     /locus_tag="PLG24_04520"
     CDS             complement(8860..>9080)
                     /locus_tag="PLG24_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HMZ24184.1"
                     /translation="HYISLALMAELFGIPTPKDDIDGSMVGKVYWEDKDLERIRVYCQ
                     KDVHTVARIYSKLTGGRYQIAEEIVEVE"
BASE COUNT         2864 a         1891 c         1827 g         2498 t
ORIGIN      
        1 gtgaatgtgc agatattaca tttacagatc agattgtaaa tggagcttgt gcaggaactt
       61 acacaatctt cagaacatac actgcaaaag actgctgtgg taacatgtca acatacacgc
      121 agaaaatcaa tgtagttgat gatactccac cgcagattat accaataatt cgtccattgt
      181 caaccctaaa atcaggtgac atcctaacga cttattgtgg tgctgatgaa ttcccaacct
      241 ggttgaatca gccggtcaac agtctgataa gtgcagtgga tgcatgttct tccaatccta
      301 aactcagcct caatatctct gaggagaatc aggatgcatg ctggctgtat ggatactcca
      361 agcaatacac tgtatctttg actgctactg atgcatgtgg caaccaaagt gaattgaagt
      421 tctatatcca ggtaaaagac acaacagccc ctcaggttat gtatctaaac gagtttatct
      481 gtgaagatga caatgctgtt cctgttgtgt ttgacaactg ctcttctctg acttacgaaa
      541 cgaacgacat cccggtaaca ggggaatgtg aaggaagtca gaatttcatc agagtgtgga
      601 ctatttccga tgcatgtcag aatacaatat atgcatctca atatgtcgtc aaaaatgatc
      661 ataaagcgcc ggttatccac atgattaatc cagcttttgc agatcatctg agtggagaca
      721 tcgtcagcct ggattgtaat aactggcagg atgtttcagc ggccgaagcc ttgacatggg
      781 tggaagcaga agacaagtgc gattatatca cgctggattt caacagtaga ttgatcaatg
      841 gcaactgtct cagcgatgga ttcaaatcaa tgtacgaata cacatggaca gcagccgact
      901 attgtggcaa tcagtcaact tttgtgttgt tcgttcaatt gacagataat acgccacctg
      961 tgttcaacac ttctctgact tcaattaatg tggattgtga gaattcaatt cctgtagtca
     1021 aagctactga caattgttct gatgtaacat tgactgtaac aagaaccaag tttgaacttg
     1081 gatgtcctaa caactttaca ttggatgaag tatatacggc aaccgatgct tgtggtaatg
     1141 tatctacatt ctcaagagtt gtgaccgtta tggatactgt aggtcctgtt atctacgttc
     1201 cggatgcgat ttgtgaaaca gaccttggca cacagaatgt ggtggcattt gattactgta
     1261 caggtaagcc ttcccatgta agtgccaatg tagatcacaa cattgtgaat tgcaatggat
     1321 caagctacta tactattact tatagtgcta ccgactattg tggtaataca tcgaccaaag
     1381 tccagaaggt aattgttgag gacaatactc ctccggtgct atctttctca tatgatatgc
     1441 aaaaccagta caacatagtg gataacacca tctatgcagg ttgtggtaac tttgagcaaa
     1501 tctttaaatt gatagacgaa tatgacgctg tagttgtatc tgacgagtgc ggaacaatca
     1561 tcactccgac atattccgaa agtgaaggtg aaaccaagtg cgaaggtgat tatattacta
     1621 aagaacattt cttcaagtgg actgcgactg atgcttgcgg caacacaagc aaccttgact
     1681 tgaaagttgt tcttcatgct cttgaaagtt atgactttag cttcgtacct gctgatacta
     1741 ctgtatattg taatgaagtt gtggcattgc caactgtact accagagttg aattgtactg
     1801 aagtaagcct gagcagctcc atcaatcttg gacaagcttc tcctgacggt tcctacatcg
     1861 aagaaagaac ctttacatat accaacttgt gtggtgagga gaccagctat actcagataa
     1921 tcaaccacac tttcaattca gatctggctt gtaatattac aattccaact gaagtatatt
     1981 gtaactccag caacaacctg tttacagtag ttgtaaccgg cggtcaggct ccttacactt
     2041 atgattggga gattttgaat ggctggtgtc atatccttgg cggacaacat actccaacag
     2101 tacagatatc aatatccttc aagacattgt atttgaaagt aaaggttacg gacgcccgtg
     2161 gatgtagtac tgaatgttat gtagaagttg attgtagcct tgataatcct ggaatcaacg
     2221 tggttgaccc gaatgtaatc gataacagct caacaattgg acagctgaat gtaatcaacg
     2281 attatactct aaggccaaat ccaacaagtg ctcaggtata tcttgacttt gaatccaaat
     2341 tggctgaaaa agtgaccatc aatgttactg acaacttcgg taaacttgtg tacaccaaga
     2401 aagtaactac tgtcaaaggg tttaatacag aactccttga aaccaaccac ctaccggtcg
     2461 gtttgtatca ggtgagtctg atatcttctg ataaactcaa gacattacag ttaattaagg
     2521 ttaaataacg tttagcctta gttaaatatg gggaagaggg atcctcgcat gaggattcct
     2581 tttcattttg aaatagaaaa ggggcattca gccccttttt tttatccaaa tagtacaaaa
     2641 tgaagttgag aaatatatta taagccggtt ttcgtggact caatatgata gtctaccaat
     2701 catttacaat acaaatttat aggactaaat ttattgaata ggccaaatta cagtccccat
     2761 gggatcgccc aaaaccggca catgttggtt gacctgaaga ttcggaaaat tgccccgcta
     2821 tatttacacg cccattttat tcatgatgtc cgcctttagc ttttcgtaat catcttcatt
     2881 gatggctccg gttgacttca tatcatacaa cctttgtaag gcatccaaat atatattggt
     2941 cgcttcgctt tgagcctgag gttgattatc ggcttcctct tttttgattt tggctgtata
     3001 ctccttgcct ttctcgaatt tttcattgta aaagtgctta aagcttttcc agtcaaaatc
     3061 caaaaaagtt cctccttctg aaaagtgcct tttaaaaact tcccctacag cataggttgc
     3121 tgctcctgaa aatgcagaag ccgcaattcc tcctacgata cttccaatca ccggtatcat
     3181 cttggcaatc attcggccac ccaatcgagc cactccaccg gaggtcaatg ccagaatagc
     3241 tgcctttcct tccgtctcct taaaatccac cttatacaga ttggacaacc ttcgtatcat
     3301 ttcaatctgt actgcggtga cagccatcaa atcaacaacc ggcacaggta tcagcccggc
     3361 tcctgatgca aaaacaatag agttgcgaat aatgctatcc gcttgttttt ctataacctg
     3421 attttcattc atattcattt attccagttt ttataagtaa ccaacataat tccaacctcg
     3481 gattgttccc ctactcgacc aatggcaata aaaaccagat taatgagact tttttttgtt
     3541 tctacaaaac aatagcctta cctttgtccg gaatttgatt ttttaaccaa aacaatgtaa
     3601 atggatcccg acccgcatga ttcgatatta cgcaaagcag caaggaacat tggtcgaact
     3661 gccggattat gccgtacatt gttggatcaa tatagaacct ccattttctc acgatgagtt
     3721 ggaagatctt tctaccaggc tcaatatacc attagacttt ttcactgacc cgcttgatat
     3781 cgatgagcgt tcacgatatg agaaggaagg gcctgcaaga cttatagtga tgaattcccc
     3841 cattctgaat gaaacagaca gggaaagcga gtctatttac attacgattc ctatcggtat
     3901 tatcttcagc cctgaaagtg tcattaccat ttgcgcacat gaaaacccgg tgctggaact
     3961 atttttggag gataaaatca agaatttcaa ccctgcaaat attcagcatt ttatattgca
     4021 gattcttgag cagaatgtat atagattcat ttcatgtctt aaaaaactca acctgaagag
     4081 gaacctgttg gagcaggaac tctatgattc ttccagaaac agtgaattat ggcaaatgct
     4141 cagaattgaa aaaagtttgg tatactttgt caactcactg aaagccaacg aattgcttaa
     4201 agtgaagatg aagaggattg attttttggg cctgggtttg aatgaggagc tggaggagat
     4261 ctatgaggac ttgatgatcg acaacagtca ggcactcgag atggccaata tctacactgt
     4321 aatcaccgga agtactatgg aagccttcgc cagcatcata tccaacaaca tgaacgttga
     4381 tattcagcgt ttgactttcg ttaccatcat gcttatggtg cctaccatca tcaccggatt
     4441 ttacggtatg aatgtaaaac tcccttttga aaattataaa tatgcatcct tcttcatcat
     4501 catcttctcg gtattagtag gagcaggttt aatttatttc ctgcgccaaa agagattcag
     4561 atcctcctaa ttttatttcg aataagcagt ttcaataact tgcgccccgg atattgactc
     4621 tatttaggta gcttaacaaa caataaaaaa gacaaacaga tacaattcgg taatcatgta
     4681 gaaataatcc ttgaaaattg agctaattac caaccaaaga ggcaattata tacgttaata
     4741 tttatatgtt ttctgaaacg taatccatat ttatcaaaaa actttgtttc tttgcaaaat
     4801 aatattaaaa tatagctgaa aatggagatc acggcaggat ttataactga ctttttgggc
     4861 ggtacgatca tcggggatag gaatgttgta atcaatggag caagtacaat tgaaggaggc
     4921 aggccgggaa ccatcagctt tctttccaac cttaaatacg aagaatatat atacaattct
     4981 gaagcttctg ccattctggt tgacaactcc tttgaaccct cctatcccgt aaaagcagcc
     5041 ctgatcaaag tggagaatgt atatgaatct ctggcaagac tgcttgacaa attcaatgtc
     5101 actacagaaa tcgagcggca tattagtccg aaagcccata tcgatgacaa ggcggttttg
     5161 ggcaacaatg ttggtgtcgg agttttttcg gttattgaag aaaatgtgcg cattggcgat
     5221 aacgtagaaa tcgaaaatca ggtaaccatc ggccagggtg taaccattgg tcagggcaca
     5281 attatcaagt caggagtgaa aattctcgaa gggtgcgtga ttggtgctaa tgtcatcata
     5341 catagtaata ctgtgatagg aagtgacggt tttggctttg cgcctaccgg ggaagggaat
     5401 tatgtcaaga tacgccagct cggcaatgtt gtcattgaag atgatgttga gattggatcc
     5461 aattgcagta tagacagggc gacatttggc agtaccatca tccgcaaagg agccaagctg
     5521 gacaatctta tccaaatcgc ccacaatgta gaaataggta gtaatactgt catcgctgca
     5581 caaaccggca ttgcaggaag cgccagaata ggtgaaggtt gtaaaattgg agggcaggta
     5641 ggaattgtag ggcatattca aatcgccgat ggaactcaaa tccaggccca gtccggtgtc
     5701 caatctgccg ttaagaataa gaacagtaag ctgttcggct caccggcact cgattataat
     5761 aattatctga gatcctatgc aatctttaaa aatctgcctg ccctggcaga aaaagtggag
     5821 aaaatagcca gaccccatct cggaaaggcc aacgagcctg acgcttgaga taaactttat
     5881 tcaatttcat attatattct tacttttgcc aaaatttata aatttaattt ctggcttaac
     5941 aacattttat gttgcaaaaa acaataaagg ataaggttag cataaaagga atcggcctcc
     6001 ataccggcaa aaccgttgaa atgaccttta ttccggctgc tgaaaaccac ggtataaaat
     6061 ttcaacgcat cgatacaccc gaacaggtta ttattccggc tgatatttta agagtagtat
     6121 caaccaatcg gggaacagcc atcagctcag gcgaaatcac catctacaca attgagcatg
     6181 cattggcggc acttacaggc cttggaatag ataacgtgct tatcgaaata aatggtcctg
     6241 aagtaccaat cctcgatgga agtgcaaaac catttgtcga tgcaatagaa caaacaggca
     6301 tcgtagaaca aaacgaagaa aaagattatt ttatcataac agaacccatc tcctacaagg
     6361 atgagactac aggaagtgaa atgatagcct taccgggcga tgatttcgag gtgactacta
     6421 tgattgattt tgcctcacct gttttgggag aacaatttgc caaactggac catattagcc
     6481 agttcaagga ggagatttca gcctgtcgta ccttcgtatt tctgcatgaa gtagagaagt
     6541 tgctcaatca agggctgatc aaaggaggcg acctggataa cgccatagtt atagtggata
     6601 gaatgatgaa ccagcaggaa ctggatgact tagcgaaaaa actgggcaaa ccaagcgtca
     6661 agatagaaaa ggaaggcgtt cttaatacgc tcaagctgca tttcagtaac gaaccggcaa
     6721 ggcataaact attggatgta attggtgaca taaccttgct gggaaggccg atcaaaggaa
     6781 aaatagttgc taccaaaccg ggacattctg tcaacgtgga atttaccaaa cttctgagga
     6841 aggctgccaa cgagcagaaa aagttgaaag gtaagccaat ctatgatcca gacaaagatg
     6901 ctgtgatgga caccacccag atcatgggta tgctgcctca taggcatcca ttccttatgg
     6961 tggacaaagt aatcgaaata aacgaaaact atgtagtcgg tgtaaagaac atatcgttta
     7021 ctgaagcatg ttttcaagga catttccccg gcaatccggt atttcccggt gtattacaaa
     7081 tcgaagcaat ggctcagacc ggtggaattc tttgtctttc tctgatgccc gatccggaaa
     7141 attgggatac ctattttcta aaaataacca acgccaaatt caaacaaaag gttgtccccg
     7201 gagatacaat ggttatcaaa atggaactcc tggaacctat acgacgtgga atctgccata
     7261 tgtacggtat tgcctatgta ggcaacaaaa ttgtatcaga agccgatctc accgctcaaa
     7321 ttgttaacag gacaaccatt aaataatgac accgaaattt tcagatatcc atcccaatgc
     7381 tcaaatagga atcggcactc agatcaacaa cttcgtaacc atagaagacg atgtacaaat
     7441 cggcgaaaat tgttggatcg gatcaggtgc cgtaatcatg aagggcgcca ggctaggcaa
     7501 taatgtccgt atatttccgg gtgccatcat tgccggaata ccgcaagacc tgaagtttca
     7561 aggagaggag tctcttgtcg aaatcggaaa caatacaaca atcagggaat atgtcacggt
     7621 caaccggggt acagcagcag caggaacaac catcattggc gaaaactgtc tgattcaggc
     7681 ctattgccat gttgcccatg attgcaaaat tggcaacaac tgcatcatgt ccaacaatac
     7741 agcccttgca ggccatgttg tactcgaaga ctatgcaata ataggaggca tgtcagctgt
     7801 acaccaattt tcacgtatag gttgccatac catgattgga ggatatagca aggttagaac
     7861 agatgtcccc ccctttatca aagcagccag ggacccgctt tcctatgcag gagtcaactc
     7921 tatcggtatg aaacgcagag gatttaccca ggaccgggta catcatatcc aggatattta
     7981 caggatcctg tttgttcatg ggaccaattt ccataaatcc atcgaacaaa tcgaaaacaa
     8041 tattccgaat acagaagaca agacttatat tctcaacttc ctcaaaagca gtaccagagg
     8101 attgatcaag ggtatttaat caaaaatacc tcttatgatt ccaaaggttg aagttatcac
     8161 ttgcaaggag attggaaaaa gatattcata cgaatgggta ttaagggact ttgattttca
     8221 atttaaaaaa ggagaatgct acgctatccg tggcccgaat ggcagcggaa aatccaccct
     8281 gctaaagatc ctcagcggtt actcgacccc ttcaaaagga aagatatgtt attcaagtgg
     8341 aaataccgac atacctacaa gtgatattta ccgacatatc gcattttcag caccctatat
     8401 tgagctgatt gatattctga ctgttgatga actgttggat atgcaccatg gtctaagacc
     8461 atttattaat atggatactg tccgtgaaac tatcaaaaat ctacctttca aaaatatgtt
     8521 ccgaaagaaa gttgaagagc tctcgtcagg aatgaaacag cgattgaaac tcatcctttc
     8581 cattatgtct caatcctctg taatcctgct ggacgaaccg gggagcaacc tggatgacga
     8641 cgggaaacaa tggtttgaag aactgctgaa gccaagaatc aaggaccaca tcgtaatcat
     8701 tgcatctaat gaagagtccg atctgcgtct ggcccaacat ctgattaaaa ttacagacta
     8761 taagaaagca aaacaatcat aggatcacct aaggtcaaaa catttcgaaa taatcaggta
     8821 acaccgccgc atttttaaaa acctccaaat aacaaacccc tactctactt ctacaatctc
     8881 ctcagcgatc tgatatcttc cccctgtgag tttactatat attcttgcaa ccgtatggac
     8941 atccttttgg caatacacac gaatcctttc cagatcttta tcctcccaat atacttttcc
     9001 caccatggaa ccatcgatat catccttagg tgtaggaata ccgaacaact cagccattaa
     9061 ggcgagagaa atgtagtgct
//
LOCUS       DAOQQN010000082         9059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2899_length_9059_cov_4.721346, whole genome shotgun sequence.
ACCESSION   DAOQQN010000082 DAOQQN010000000
VERSION     DAOQQN010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9059
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2899_length_9059_cov_4.721346"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..>2272
                     /locus_tag="PLG24_04525"
     CDS             15..>2272
                     /locus_tag="PLG24_04525"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HMZ24185.1"
                     /translation="MSEPKEKLKYKSNNVLTDNDTVIESNVLGKFFPAVPFQAKSAEK
                     NDFEEEINNETEAQENHSLNFSSAAPPDDRITNHLFPNIVRTDSGIQLKANSNYTTPL
                     QLKPNKTKISSYSAPAFQLKTVEPTQNDSKEDDTSSVQNTSHSNSLPKDVLGNMEQMM
                     GADFSGVNIHQNSTNAHNVGALAYTQGEDIHFAPGQFDPTSKSGKELIGHELTHVVQQ
                     RQGRVQPTTQAKGLPVNDDEGLEAEADEMGRQAAQMKANPDAPPAQMSLFGNEQERIR
                     TKTGIKINNPTFDESRMRQELPRTSFTGAGDPRLAQAMTVLYSGATEGAEVEQSLRTI
                     AEIRGLDVSTVNEQYNRAIAIRTAGRQYANPNGAANDANDPSPDLDLDRHPDFTGSLS
                     QLRFGSTLGDVFGIDPVFGALISPTGGLVGPGNDSVSGDANNPTVLHGTVHDAAGYLL
                     NAHGVGPGYNYLKRSWELDATNPLAGQTSGTAYWADRNPIAQATATLGTFGVNAVMDN
                     PSILLGMIHPGLAVPGLMNAGRDYENNFIRSAVEVGGGNWASRGVDSISNTLNAGWNA
                     ASNLWDGVTGVASDVWDGARGVTSDVIGGMRGVASDVWSGARGVASDVWDGARGVTSD
                     VIGGMRGVASDVWSGARGVASDVWDGARGLASGVMGGIGGALKDSGIGQPLTAPGLGG
                     IASGLGRALSRAASGVGGALRDAGIGQPLTAPGVGGIVGGIGRALSGVTGGIGGALRD
                     AGIGQPLTAPGVG"
     assembly_gap    2273..2372
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2373..4387
                     /locus_tag="PLG24_04530"
     CDS             <2373..4387
                     /locus_tag="PLG24_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24186.1"
                     /translation="GLNGGIFGGLGGLLPGLLGPSGGILGGLGGLLGKGGIGQPLTTP
                     GMGGILGGLGGILGRLGRILPGITGGLSGTLKDSGIGQPLITPGLGGLGAAAGRRLSG
                     LSDGQGDALRDAGIGQPLTTPGMGGILGRLGRILSRITGGLSGALKDSGIGQPLIAPG
                     LDGLGAAAGRRLSGLSDGQGDALRDAGIGQPLTTPGMGGILGRLGRILSRITGGLSGA
                     LKDSGIGQPLIAPGLDGLGAAAGQRLSGLSDGQGDALRYAGIGQPLTSPGMGGILSGL
                     RRIINSIPEAKNVSANPTNHPGWDLPFDPFRIGGIGVPNNTPTHIPAGVGSKPEGDGY
                     STPWLYSGFKPSADGKYPIGYSGGFGLGRLAAPGNTNTYGAADINLGAWDDPDGGTRY
                     GLGASAGAGKGSYDIPAIINSFYPGAMDKRNILNLDVGLGTAGADLSLNPDTGFSIGA
                     QANIAEGAITAGTTDTGDSDKTYKLGLSEGVGAALRGHWGDKDKDGLREYGFGFDFGP
                     LSVDMKTEDPLRDLGIGFIPGVGPALANLLPGGNLTEGLANKFGLTTRNATLADTWNL
                     VSDTASDAWDGAKGLASDAWDSVTSTASDAWDGITNTASDAWDGVTSTASDAWDGAKG
                     LASDAWDGAKSTASDAWDSATSTASDAWSGTKSIASDAWDGLKSLF"
     gene            4442..4900
                     /locus_tag="PLG24_04535"
     CDS             4442..4900
                     /locus_tag="PLG24_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24187.1"
                     /translation="MKTLNANLSEGLQGLNFFGYFFLIGGIFFLVIAIMKIRMLFPYI
                     GIFAVIIVGYSMLSKSKKTLERRQTCFTNGIGIEGKVVKQGRAMNPFSSTSKYTITVK
                     TGDKSIEITHKSKDLWASCKIGDTIIGLKNNEEYFFGPEIGCIFKYFEPK"
     gene            4912..5205
                     /locus_tag="PLG24_04540"
     CDS             4912..5205
                     /locus_tag="PLG24_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24188.1"
                     /translation="MNIYYKKPVITEEKRVIIEQVYELGEEILVNGIIRKELKVIVEI
                     YPSIELLLETISIATMNYNNVSLHGHKTVMQLEMEKKALVDLQVMLSEMLDKK"
     gene            5467..6804
                     /gene="ffh"
                     /locus_tag="PLG24_04545"
     CDS             5467..6804
                     /gene="ffh"
                     /locus_tag="PLG24_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HMZ24189.1"
                     /translation="MFESLNEKLEGAFKSLKGEGKLTELNVATSIKEIRRALVAADVN
                     YKIAKEFTDKVKDQAMGEKNVLKAVKPGELMVKIVMDELIELMGGASEGISLSGNPAI
                     ILISGLQGSGKTTFTGKLSLYLKTKRSKKVLVTAGDVYRPAAIDQLTVLSQQVGVDIY
                     KEPENKNPVQIATNAIQYAKDNGFDVVIIDTAGRLAIDEAMMNEIEAIKKAVNPSETL
                     FVVDSMTGQDAVNTAKAFNDKLNFDGVVLTKLDGDTRGGAALSVKYTVGKPIKFISTG
                     EKMEDLDIFYPDRMAQRILGMGDIVSFVERAQQQFDEEEAARIEKKIKKNKFDFNDFL
                     SQINQIKKMGDIKSLMNMIPGMSKLTANLDIDEKAFSKVECIIQSMTPQERANPELLN
                     PSRKRRIAKGAGKDINEINMFIKQFDQMKNMMHNMSIGKMGNMGMPKMGRFRR"
     gene            complement(6815..8203)
                     /locus_tag="PLG24_04550"
     CDS             complement(6815..8203)
                     /locus_tag="PLG24_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HMZ24190.1"
                     /translation="MNIYLHLAYLTASILFVIGIKMLNKTKTARNGNLVSAVGMLIAI
                     LATVSQVQSITYIDIFVAFLIGSAIGLIFAYKVKMTQMPEMVAIFNGFGGLASVAVAL
                     SDYWLHTQQQSIELSSITVVSIILSIFIGGITFTGSMIAFLKLNGSISGRAIVFKGQN
                     PLNLMLLIIGIILSVLIVMYTSDPSYILLLTILSLVLGVLVVIPIGGADMPVVISLLN
                     SYSGMAACATGFVLNNNVLIIAGALVGASGIVLTDIMCKAMNRSLINVLLGGFGQTSS
                     SVQAGSSQDIVIKEVGVEEAAQIFDSVSSLIIIPGYGMAVAQAQHAIRELYELCEKKG
                     VSVRFAIHPVAGRMPGHMNVLLAEANIPYDKLVEMEAINDDFTNTDLVFVVGANDVVN
                     PAAKNSPGSPIYGMPVLNAELARTVIVSKRGMNPGYAGIENELFGADNCLMLFGDAKQ
                     TITKLVAELKEL"
     gene            complement(8225..8512)
                     /locus_tag="PLG24_04555"
     CDS             complement(8225..8512)
                     /locus_tag="PLG24_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HMZ24191.1"
                     /translation="MSIDVLLPLMYILVLGTFLGFELISKVPPTLHTPLMSASNAISG
                     ITVVGAILACNELGFTSLSKWLGLAAMVLATVNVVGGYAVSDRMLKMFKKK"
     gene            complement(8519..>9059)
                     /locus_tag="PLG24_04560"
     CDS             complement(8519..>9059)
                     /locus_tag="PLG24_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HMZ24192.1"
                     /translation="TKEQVESLGGRFLSVESDAEVKTEGGYAREVSAEFLQKQQQLIE
                     ERIKEADLVITTALVIGKKSPILVTEKMVDSMKKGSVIIDMAVEWGGNCELSEMNKTK
                     VYKGVTIIGEANLPSMLPINASQLYATNISTLLMHLASPEGLNFDREEEIAKGCLITI
                     DGSTVHEFTNKILNASSNK"
BASE COUNT         2614 a         1878 c         2133 g         2334 t
ORIGIN      
        1 tttaaaacaa catcatgtct gaacccaagg aaaagttgaa gtacaaatcc aataatgtac
       61 ttacagataa tgatacagtc attgagtcaa atgttttggg taaattcttt cctgcagtac
      121 catttcaagc aaaatcggca gagaaaaatg atttcgaaga agaaataaat aacgaaactg
      181 aagcgcagga aaaccattct ttaaatttct catccgcagc accgcctgat gacagaatta
      241 ccaaccactt atttccgaat atagtaagaa cagattccgg tattcaacta aaggccaatt
      301 caaattatac aactcccctt cagctaaaac ccaataaaac aaaaatcagc agttattctg
      361 ccccggcttt ccagcttaaa accgtagagc caacgcaaaa tgacagtaag gaagatgata
      421 cttcctctgt acagaatact tctcattcaa acagcctacc gaaggatgtt ctgggcaata
      481 tggaacaaat gatgggtgcc gatttttcag gagttaatat ccatcaaaat tctaccaatg
      541 ctcataatgt aggagcacta gcttataccc aaggtgaaga tatccacttt gctcccggcc
      601 agtttgatcc aacttcgaaa tcaggaaagg aattgattgg acacgaattg acacatgtcg
      661 tacaacagcg ccagggacgt gtccagccca ctacccaagc taagggtctt cctgtaaatg
      721 atgacgaagg tctggaagcg gaggcagatg aaatgggacg ccaggcagca caaatgaaag
      781 caaatcctga cgcacctcct gcccaaatga gtttatttgg caatgagcag gaaagaataa
      841 ggactaaaac cggaattaaa atcaataacc caacatttga cgaaagtcgg atgcgacagg
      901 aattgccaag aacttccttc acaggtgcag gtgatccaag gttggcgcaa gccatgactg
      961 ttctctattc aggtgcgact gagggtgctg aggtggaaca atcactcagg acaattgctg
     1021 aaatcagagg attagacgtt tctactgtca acgagcaata caatcgtgcc atagctatca
     1081 ggacagctgg tcgtcaatat gccaatccaa acggtgctgc caatgacgct aatgacccat
     1141 ctcccgatct ggatttagat agacaccctg acttcaccgg gtcactttct caactgagat
     1201 ttggcagtac tttgggcgat gtttttggga tagatcctgt ttttggagct ttgatcagcc
     1261 caacaggtgg tttggtcggt cctggcaatg attctgtttc tggcgatgcc aacaacccga
     1321 ccgtccttca tggcactgtt catgatgctg ccggctattt attaaatgct catggcgtgg
     1381 gccccggata taactacctc aaaagatcct gggaattaga tgcaactaat cccttagccg
     1441 gtcagacttc cggaactgcc tattgggccg atagaaatcc aattgctcaa gctactgcga
     1501 cattaggaac atttggagta aatgcagtca tggataaccc atctattctg ctagggatga
     1561 ttcatcccgg gttagcagta cccggattaa tgaatgcagg cagagattat gaaaataact
     1621 ttattagatc ggctgttgaa gttggtggcg gcaattgggc atccaggggc gttgacagca
     1681 tatccaatac cttgaatgct ggatggaatg ccgcatcgaa cttatgggat ggtgttactg
     1741 gagtcgcctc cgatgtttgg gatggcgcca gaggtgttac ttctgatgtt atcggtggca
     1801 tgagaggagt ggcttctgat gtttggagtg gcgccagggg agtagcttcc gatgtttggg
     1861 acggtgcaag aggtgttacc tctgatgtta tcggtggcat gaggggtgta gcttctgatg
     1921 tttggagtgg cgccaggggg gtagcttctg atgtttggga cggtgcaagg ggtttagcct
     1981 ctggggtaat gggaggtatt ggtggcgctt tgaaggattc cggcatcggg caaccactga
     2041 cagcacccgg attaggtggt atagctagcg gtttaggtag agccttgtct cgtgcagcca
     2101 gtggcgttgg tggcgctttg agggatgcag gcataggaca acccctgaca gcacccggcg
     2161 tgggtggtat agttggcggt ataggtagag ccttgtctgg tgtaaccggt ggcattggtg
     2221 gcgctttgag ggatgcaggc ataggacaac cattgacagc acccggcgtg ggnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcggctta aatggcggaa tatttggtgg
     2401 actcggaggt ctattaccgg gtttattggg tccttccgga ggcattttag gtggattagg
     2461 aggattatta ggtaaaggcg gcatcggaca accactgaca actcccggca tgggaggtat
     2521 attaggtgga ttgggaggta tattgggtag attaggtcgt attttgccag gaataacagg
     2581 tggacttagt ggcactttga aggattccgg tatcgggcaa cccctgataa cacccggatt
     2641 aggtggcttg ggagccgctg caggccgaag gttgtcggga ctatccgatg gacaaggaga
     2701 cgcgttgaga gatgccggca tcggacagcc tctgacaact cccggcatgg gaggtatatt
     2761 gggtagatta ggtcgtattt tgtcaagaat aacaggtgga cttagtggcg ctttgaagga
     2821 ttccggtatc gggcaacccc tgatagcacc cggattagat ggcttgggag ccgctgcagg
     2881 ccgaaggttg tcgggactat ccgatggaca aggagacgcg ttgagagatg ccggcatcgg
     2941 acagcctctg acaactcccg gcatgggagg tatattgggt agattaggtc gtattttgtc
     3001 aagaataaca ggtggactta gtggcgcttt gaaggattcc ggtatcgggc aacccctgat
     3061 agcacccgga ttagatggct tgggagccgc tgcaggccaa aggttgtcgg gactatccga
     3121 tggacaagga gacgcgttga gatatgccgg catcggacag cctctgacat ctccaggcat
     3181 gggaggtata ttgagtggat tacgaaggat tattaattca atacctgaag caaaaaatgt
     3241 ctcggcaaat ccaacaaacc acccgggctg ggacttaccc tttgatccat tcaggatagg
     3301 aggaattgga gtcccaaaca atactccgac acatataccg gctggcgttg gcagcaaacc
     3361 tgaaggtgat ggctattcaa caccttggtt atattccgga ttcaaaccaa gcgctgatgg
     3421 caagtatcca ataggctact caggtggatt tggattgggt aggcttgccg caccgggcaa
     3481 cacaaatact tatggggctg ctgatataaa cttaggcgct tgggatgacc ctgatggcgg
     3541 cacaagatat ggcttaggtg catctgcagg agcaggtaag ggttcttatg atattcctgc
     3601 aataattaat tctttttacc cgggagctat ggataaaaga aacatattaa accttgatgt
     3661 tggattgggt acagctggtg ctgatctatc cctaaaccct gacacaggat tttcaatagg
     3721 cgcacaggcc aatattgctg aaggagcaat tactgcagga acaaccgaca ccggagactc
     3781 ggataaaacg tataaacttg gactaagcga aggagttggt gcagcattac gtggccattg
     3841 gggtgataag gacaaagatg gccttcgtga atatgggttt ggctttgatt ttggaccgct
     3901 ttcagtcgat atgaaaactg aggatcctct tcgagactta ggaattggat ttatacccgg
     3961 ggttggacca gcactagcta atttattgcc tggcggtaat cttacagaag gcctcgcaaa
     4021 taaatttgga ttaacaacaa gaaatgcgac attggctgat acttggaatc tggtatcaga
     4081 tactgcctct gatgcatggg acggagcaaa agggctagct tctgatgcct gggatagcgt
     4141 gacaagtacc gcttctgatg catgggacgg aataacaaac actgcctctg atgcatggga
     4201 cggcgtgaca agtactgcct ctgatgcctg ggatggagca aaaggattgg cctctgatgc
     4261 gtgggatggc gcgaagagta ccgcttccga tgcatgggat agtgctacaa gtactgcctc
     4321 tgatgcatgg agtggtacta aatcaatagc ttccgatgca tgggatggct taaaaagttt
     4381 gttttaaaat ttagaaatta tgtttaggtg ggtttttatt tcggaaaatg tacccagaga
     4441 tttgaagaca ttaaatgcaa atctaagcga aggactccaa ggcttgaatt tttttggata
     4501 tttctttttg attggaggta tatttttctt agtaatagct ataatgaaaa taagaatgct
     4561 ttttccatat attggtattt ttgctgtgat aatagttgga tactccatgc tatcaaaatc
     4621 gaaaaagacg ttggagcgaa ggcagacttg ttttacaaac ggaattggaa tagaagggaa
     4681 ggtggtcaaa caaggaaggg caatgaatcc tttttccagt acttcgaaat ataccatcac
     4741 agtaaagact ggcgataagt ctattgagat aacccataag tctaaagacc tttgggcttc
     4801 ttgtaaaatt ggggacacaa ttattggatt gaaaaataat gaggaatatt tttttggacc
     4861 tgagataggg tgtattttta aatatttcga accaaaataa gcgatataaa aatgaatatt
     4921 tactacaaaa agccggtcat taccgaagaa aaaagagtaa ttattgaaca ggtatatgag
     4981 ctcggagagg aaatacttgt aaatggaatt atcagaaagg aattgaaagt aatagtggaa
     5041 atttaccctt ctatcgaatt attgcttgaa acaatatcga tagcaaccat gaactataat
     5101 aatgtttccc tgcatggtca caaaaccgtg atgcaattgg aaatggaaaa gaaagcatta
     5161 gtggatttac aagtaatgtt atcggaaatg ctggataaaa aataaccggt ttgcttttac
     5221 ccggcaaatt gacatttcgt tcgttttttg ttcaatactt ccggcttctt gatttttttt
     5281 atttaaacct tgaccttcaa gttttaaaca aagttgcata tgccttgtat taataacctg
     5341 cctagctgaa ctttacacta catttggcca tggattctcc tgttcggatt atcgaatcat
     5401 tatttctata tactttcccc tctctatttt ttaactatat ttgcacctca aaattgaaag
     5461 acagacatgt ttgaaagttt aaatgaaaaa ctggaaggtg ccttcaaatc actaaagggt
     5521 gaaggcaagt taaccgagct taatgtcgca acctccatca aggaaattcg tcgtgcattg
     5581 gttgctgccg atgtcaatta taagatagcc aaggaattta ccgataaggt caaagatcag
     5641 gctatgggtg aaaaaaacgt cctgaaagcc gttaaaccgg gtgagctcat ggtcaaaatc
     5701 gtcatggacg aattgatcga gttgatgggc ggggcatccg aaggtatttc actttccggc
     5761 aatccggcca ttatccttat ttcgggattg caaggttcgg gaaagaccac ttttaccggt
     5821 aaactttccc tttatttaaa aactaaacgt agtaaaaaag tacttgtaac agcgggtgac
     5881 gtgtatagac ctgctgcgat tgatcagctg accgtgctca gtcagcaggt tggtgtagat
     5941 atttacaagg aaccggagaa taaaaatcct gttcaaatcg ccactaatgc catccaatat
     6001 gccaaggaca atggttttga tgttgtcatc atcgatacag ccggccgtct cgccatagat
     6061 gaggctatga tgaatgaaat cgaagcgatt aaaaaagcag tcaatccgtc tgagactttg
     6121 tttgtggtgg attccatgac aggacaggat gctgtcaaca cggctaaagc tttcaacgac
     6181 aagctcaatt tcgatggcgt cgtccttacc aaacttgacg gtgatacacg cggtggtgcc
     6241 gccctttcgg ttaaatatac tgttgggaag ccaattaaat tcatctccac cggtgagaaa
     6301 atggaagatc tcgacatctt ctatccggac aggatggctc aacgaattct gggcatggga
     6361 gatatcgttt catttgttga acgtgctcag cagcagtttg atgaggaaga agcagcccga
     6421 atcgagaaga aaatcaagaa gaataagttt gacttcaacg acttcctctc tcagatcaat
     6481 cagataaaaa agatgggtga catcaaaagt ctgatgaaca tgattcccgg catgagcaaa
     6541 ctgactgcca acctggacat cgatgaaaaa gccttcagca aggtggagtg catcatacaa
     6601 agtatgactc ctcaggaacg tgccaatcct gaattgctca acccaagccg taaacgacgt
     6661 atagccaagg gggccggtaa agacatcaac gaaatcaata tgtttatcaa acagtttgat
     6721 cagatgaaaa acatgatgca caatatgtcc ataggtaaaa tgggcaatat gggcatgcca
     6781 aaaatgggac gattcagaag ataaccttac gttgctataa ttccttcaac tctgcaacca
     6841 acttagttat ggtctgcttg gcatcaccaa agagcatcag gcaattgtcc gcgccaaaca
     6901 attcattctc gatgccggcg taacccggat tcatccccct cttggataca atcaccgttc
     6961 tggccaattc cgcattcagt accggcatac catagattgg gctacccggt gaattcttcg
     7021 cagcaggatt gactacatca ttagccccaa cgacaaatac aaggtcagta ttggtaaaat
     7081 catcattgat ggcttccatt tccaccaatt tgtcatacgg gatattggct tcggcgagca
     7141 aaacattcat gtggccaggc atgcgaccgg caaccggatg tatggcaaag cgcacactga
     7201 cacctttctt ttcacacaat tcatagagct ctctgatggc atgctgagcc tgtgccacgg
     7261 ccattccata tcccggaata ataatcagcg aacttactga atcgaagatc tgggcagctt
     7321 cctccacgcc cacctcttta atcactatat cctgactgct tcctgcctgc acagatgacg
     7381 atgtctgtcc aaatccgcct aaaagaacat tgattaatga tcgattcatt gccttgcaca
     7441 tgatatccgt aaggacaata cctgaagctc cgacaagggc acctgcaatg atgaggacat
     7501 tgttgttgag aacaaatcca gtcgcacaag ccgccattcc cgaatacgag ttgagcagtg
     7561 aaataaccac aggcatatcg gcacctccta tgggaatcac aaccaatacg cccagtacca
     7621 gagaaagaat cgtcagaagt aatatgtagg atgggtcgga ggtgtacatc actatcaaaa
     7681 cagaaagaat aatgccaatg atgaggagca tcaaattgag tggattctga cccttgaaga
     7741 ctattgcccg accactgata ctaccattga gcttgagaaa tgcaatcatg gaaccggtaa
     7801 aagtaatccc tccaataaaa atactcagga ttatggaaac cactgtgata ctgctcaatt
     7861 caatactttg ctgctgtgta tgcaaccaat aatcagatag agcaaccgcc acggatgcca
     7921 gaccaccaaa accgttgaag atagctacca tctcaggcat ctgggtcatc ttaactttat
     7981 aggcaaatat cagacctatc gccgacccga ttaaaaaagc aacaaaaata tcaatataag
     8041 ttatcgactg aacctgtgaa actgttgcta aaatggctat caacataccc acagcggaaa
     8101 cgagattccc atttctcgct gttttcgttt tattcaacat cttgataccg atgacaaaaa
     8161 gtatcgaagc ggtcagatag gctaaatgta gataaatatt catatcagga agggaatggt
     8221 gaggttattt ctttttgaac attttaagca tacgatcaga cacagcataa ccaccgacga
     8281 cattgaccgt agcaaggacc atcgcagcca ggccgagcca tttgcttagg ctggtaaatc
     8341 ccagctcatt acaggcgagg atagcgccga caacggttat gccggagatt gcattggaag
     8401 ctgacatcaa aggcgtatgt aatgttggcg gcaccttgga aatcaattcg aagcccaaaa
     8461 aagtccctaa caccaaaatg tacatcaatg gtagtaatac atcgatactc atattattct
     8521 atttgttgct tgaagcgttt aagattttat tcgtaaattc atgaacagtg ctgccatcga
     8581 tggttatcag acaacctttg gctatctctt cttcccgatc gaaattcaac ccttcgggtg
     8641 aagccagatg catcaaaagt gtgctgatat tggtcgcata gagttgactg gcattaattg
     8701 gaagcatgga aggcaggttg gcctcaccaa tgattgtgac acccttgtat accttggttt
     8761 tattcatttc tgataattca caattaccgc cccactccac tgccatatcg atgatgacag
     8821 accctttttt cattgaatcg accatctttt cagtcaccaa tatcggagac ttcttcccta
     8881 tgactaatgc tgtggtaata accagatccg cctcctttat tctttcttct ataagttgct
     8941 gttgcttttg aagaaattcc gcggaaactt ccctggcata gccgccttct gtcttaacct
     9001 ccgcatcact ctcgacacta agaaatcttc cccccaaaga ctctacctgc tccttggtt
//
LOCUS       DAOQQN010000083         8990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2942_length_8990_cov_5.893900, whole genome shotgun sequence.
ACCESSION   DAOQQN010000083 DAOQQN010000000
VERSION     DAOQQN010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8990
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2942_length_8990_cov_5.893900"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PLG24_04565"
     CDS             <1..627
                     /locus_tag="PLG24_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HMZ24193.1"
                     /translation="YYLIGSVVGPDPYPDMVARFQSVISEEIQSQLSEKTGRSYPAKI
                     IACVGGGSNAAGAFYHFLDNPSVTLVAVEAAGKGIDTGHTAATSRVGSVGIIHGSKTL
                     LMQTDDGQIIEPYSISAGLDYPGIGPLHAHLFETGRLTMLHATDDEALDAAFELTRLE
                     GIIPALESAHALAALPKMKFVKNECVVVNLSGRGDKDMNTYIQSKNNE"
     gene            620..1390
                     /gene="trpA"
                     /locus_tag="PLG24_04570"
     CDS             620..1390
                     /gene="trpA"
                     /locus_tag="PLG24_04570"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HMZ24194.1"
                     /translation="MNRIDQLFQSKRNVLSIYVTAGFPEVNDTMRIVTHLASAGVDLI
                     EIGMPFSDPLADGETIQHSSRIALKNGITVARILDQVKIIRQTISIPIVLMGYYNQVL
                     RYGDERFFRDAKDAGADGVILPDLPVAVFQEKYMALLLDLDLKMPFLITPQTTTERIR
                     LIDSVSTGFLYIVAQYSTTGGDNWEINKQTAYFEQVRDMHLKNPTMVGFGISSKDKLL
                     AACSYANGGIIGSAFIRAIENSSNLEQTINQFIQKFKQ"
     gene            1387..2427
                     /gene="aroF"
                     /locus_tag="PLG24_04575"
     CDS             1387..2427
                     /gene="aroF"
                     /locus_tag="PLG24_04575"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HMZ24195.1"
                     /translation="MIIQLDKNITQEQRQFLVEELNRLSISPNFVSTQFTNYIVGIPK
                     KEFDIRLIGNLPGIADIHFVPDNSKLVSRKWKTKNTIIDLGDEVKIGGDELAIMAGPC
                     SIESESQIESIVQHLVRNNVRIMRGGVFKPRSSPYSFRGLGIDGLKLFYKHCAANKIK
                     IITEVMEADQIEEMYDYIDVYQVGTRNSQNFNLLDALGRVDKPVMIKRAMSGNIEELL
                     SSAEYVFSNGNEKILLCERGIRTYEKIYRNTLDLNAVPILKEKSHLPVIVDPSHGIGI
                     RAHVADMALAGVMAGADGIIVEIHEYPEKAFSDGQQTLNYQMASGLYSALLKTKKLRE
                     ELSLEMANGHSY"
     gene            complement(2574..3095)
                     /locus_tag="PLG24_04580"
     CDS             complement(2574..3095)
                     /locus_tag="PLG24_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMZ24196.1"
                     /translation="MKKSDIRELPEYYDYYILLNYDIELNDAFERSIKEINTLDVDRL
                     KRIGLRTYAEGKWSTHTIIQHLTDWERIWCYRLLLSVRNEGTIPPGLDHNLMADHANA
                     DDLSIDQLLDELRAVRMATKGMFNNFDERILRSDCKFNKTQMSVLAMGFNILGHQIHH
                     FNIIRERYLPLAD"
     gene            complement(3564..4118)
                     /locus_tag="PLG24_04585"
     CDS             complement(3564..4118)
                     /locus_tag="PLG24_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMZ24197.1"
                     /translation="MKGWLAEIDIITQSFNDKFGHLGEKELNWKPNARTWSIAQNIDH
                     LMVVNETYFPVLASLKEGAYKPPFIAKLDFMVSFIGKTVLRAVNPDRKKRMKTFPIWE
                     PDLSQVKGDILQSFKNHQNELKHQIETSNELIEKGTIISSPANRNIVYKLETAFDIIV
                     AHERRHLEQASEILNNLNIANCNN"
     gene            complement(4223..4852)
                     /locus_tag="PLG24_04590"
     CDS             complement(4223..4852)
                     /locus_tag="PLG24_04590"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ24198.1"
                     /translation="MKWYDFFSGFYDSSLEKLYYSSRKRAVELLDLKDNQIVIDIACG
                     TGANFKHIIAANDKVTLYGTDYSRGMLKKGQKIIEGNNWENVFLFQADARSLNISSIE
                     QFTNKKLSFDRVICVLGLSVIPDWEMVLDNALRLLNEGGKIVVVDVFAEKRNFNTWLV
                     EKIAKADLNRNIWQTLQTKTVNFHQEYLPVKENKVGGKLFVAVGTKQGN"
     gene            complement(4864..5340)
                     /locus_tag="PLG24_04595"
     CDS             complement(4864..5340)
                     /locus_tag="PLG24_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ24199.1"
                     /translation="MLFKKTQTGFMKLIPISLNEDKTNEAFASTDCQHLLRMYDDFYP
                     QIGFNTPWIGYFVVRHNTIVGACSFVGQPQDGKVEVSYWTFKAFEGQGIASFACRELL
                     NIAYKTDPGIIITAKTAPEHNASTKILENNHFVFTEIVQDHEIGDAWLWTHSRTED"
     gene            complement(5489..6364)
                     /locus_tag="PLG24_04600"
     CDS             complement(5489..6364)
                     /locus_tag="PLG24_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMZ24200.1"
                     /translation="MEHHWLDTTEYPFTSKYFDINGHKLHYLDEGQGDTILFVHGTPS
                     WSFDYRNIIKKLKSNYRCIAIDHIGFGLSDKPEHYDYTTQNHSRTLERFVLEKQINSV
                     TLVVHDFGGPIGLNFAIQHPERIKRLVILNSWLWSSETDPDFIKMSKILKSPLLPFLY
                     RYLNFSPKFILPKSFGDHKISGHLLKQYTRPFADKTQRNGALAFAKSLLNDQDWFEQL
                     WKKRLAISDKPTLFVWGMKDPVIKPQYLDKFQTGFTNSRTIQLDTCGHFPQEEQPEKV
                     TESILDFLTDKYNHR"
     gene            complement(6424..6990)
                     /locus_tag="PLG24_04605"
     CDS             complement(6424..6990)
                     /locus_tag="PLG24_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ24201.1"
                     /translation="MELYNFFNHFHAITMAEYNLLGDNLKTKSFKKGDCITVPGQVQR
                     ELYFVKSGVQMSYFDADNKTQVIAFTYPPGLCAIPESFSFQMPSKYFLTCLTKTELEY
                     ITYDELQKVFDQSQQIERLFRRMTEAVLAGMINRHIELHSMTIEERYKTFCQRSSHLL
                     EFVPHKYIASYLGIDPTNFSKLFNSVKF"
     gene            complement(7293..8843)
                     /locus_tag="PLG24_04610"
     CDS             complement(7293..8843)
                     /locus_tag="PLG24_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HMZ24202.1"
                     /translation="MKRTIKYYRIIWLKYDLPAGLSVFLVALPLCLGIALASGAPLYS
                     GILSGIIGGLVVSLISGSHLAVSGPAAGLTTVVAASIVSHGDYKIFLLAVIIAGLFQL
                     ILGLLKLGVIANYFPSAVIKGMLAAIGIILISKQIPVALGYDQPDFWAGGILKLFSTD
                     QFVGNINDFNQHITRGAILITGISIIVMGLLQRPFANKLKILPAPLVIVVIGILTNTA
                     FESLSSELSLKPTQLVNIPSDVFANISFPDFTKLFTSSGLLKDGLTIGMLATLETLLC
                     IEAIDKLDRRNRITPVNRELVAQGIGNMACGFLGAIPITAVIVRGSANVDAGARTKLS
                     AFTHGLFLLLSVLFIPFLLNKIPYASLSAILIVTGYNLAKPKLFRNMWSLGLKQFLPF
                     MITIFAILLTDLLIGVTIGLVLSFYYIIRNNFKEEYKITETVENGIDTFLIKLNSNVT
                     FLNKVNLRKALDDVPEKSKLTIDGSECNFIDYDILEIISQYDNKAKDRNIKLNLKGIK
                     KVNVTAVH"
BASE COUNT         2649 a         1805 c         1796 g         2740 t
ORIGIN      
        1 tattatctga tcggttccgt ggtggggccc gatccttatc ccgatatggt agcgcgattc
       61 cagtctgtca taagtgaaga gattcaaagc cagttgtccg aaaaaacagg cagaagttat
      121 ccggctaaaa tcatcgcctg tgtcggggga ggaagcaatg ctgcaggggc attttaccat
      181 tttctcgaca atccttccgt tacccttgtg gcggtagaag ccgccggaaa aggcattgat
      241 accggacaca cggcagctac gagccgggta ggctccgtcg gcataattca tggaagcaag
      301 accttgttga tgcagacaga tgacggccag atcatcgaac cttactctat ctcggcaggc
      361 ctggattatc cgggtatcgg gcctctgcac gcccatttgt ttgaaacagg cagattgacc
      421 atgcttcatg caaccgatga tgaggcactg gatgcggcct ttgaacttac acggctggaa
      481 ggcattattc ctgccctgga atcagcgcac gccctggctg cccttcccaa aatgaaattt
      541 gttaaaaacg aatgtgtcgt ggtcaacctt tcaggacgag gagataaaga catgaataca
      601 tatatccaat cgaaaaataa tgaatagaat agaccaactt tttcagtcaa aacgtaatgt
      661 tttaagcatt tacgtaactg caggcttccc tgaagtaaat gatacgatgc ggatagtcac
      721 acatcttgcc tctgccggag tggacctgat tgaaataggg atgccttttt ccgaccctct
      781 tgccgatggc gaaaccattc agcacagcag ccggattgct ctgaaaaacg gaattacggt
      841 tgcccggatt ctggatcagg ttaagataat ccgtcagact atttctatcc cgattgtttt
      901 aatgggctat tataatcagg tacttcgcta tggagatgaa cgattcttca gagacgccaa
      961 agatgccgga gcagatggag tgatcttgcc tgacctgcct gtagccgttt ttcaggaaaa
     1021 atacatggca ttgctgcttg acctggattt gaaaatgccc ttcctgatca ccccacagac
     1081 tacaaccgaa aggatccgtt tgatagattc tgtttctaca ggatttttgt acatcgtggc
     1141 acaatattcc actacaggtg gcgacaattg ggagataaat aaacaaactg cctatttcga
     1201 gcaagtgaga gatatgcacc tgaaaaaccc taccatggtt ggttttggta tttcatcaaa
     1261 ggacaaactt cttgctgcat gctcttatgc aaatggcgga attatcggta gtgccttcat
     1321 ccgggctatt gaaaacagta gcaacttaga acaaaccatc aatcaattta tacaaaaatt
     1381 taaacaatga ttattcaatt agataaaaat attactcagg aacaacgaca gtttctcgtt
     1441 gaagaattga acaggctatc catttcgcca aactttgttt caactcagtt tactaactat
     1501 attgtaggca ttccaaagaa ggaatttgac atacggctta tcggcaatct gccgggaata
     1561 gctgacattc actttgttcc cgataattcc aaattagttt ccagaaaatg gaaaacaaaa
     1621 aataccatca ttgatttagg agatgaagta aagatcggag gtgatgaact ggctattatg
     1681 gcaggtcctt gttcaataga aagtgaaagt cagatagaaa gtattgtaca acatctggtc
     1741 agaaataatg tgcgtattat gcgaggtgga gttttcaaac cacgtagctc gccctattca
     1801 ttccgcgggt taggaataga tggcttaaaa ttgttttaca aacactgcgc tgcaaataag
     1861 atcaaaatca ttaccgaagt aatggaggca gatcagatag aagaaatgta cgactatatc
     1921 gatgtatatc aggtcgggac ccggaattct caaaatttta atctactgga tgctcttggg
     1981 cgggtagata agccggtgat gatcaagcgc gctatgtcag gcaacatcga agaattattg
     2041 agttctgccg aatatgtatt ttctaatggc aacgagaaaa ttttgctgtg tgaaagagga
     2101 atacggacct atgaaaaaat ttatagaaat accctggatt taaacgctgt tcctatactg
     2161 aaagaaaaat cccatctgcc tgttatcgtt gatccttctc atggcatcgg aatccgtgcc
     2221 catgtcgcag atatggcatt ggcaggcgtg atggcaggtg cagatggaat cattgtcgaa
     2281 attcacgaat atcccgaaaa agctttctcg gatggacagc aaacactgaa ctaccagatg
     2341 gcttccggac tgtattctgc actcttaaag acaaagaaac tgagagaaga actttccctg
     2401 gaaatggcga atggacacag ttactaataa ccgggataga tcaaaatcaa tttgctgatg
     2461 gcgaaatgtt tcgactgcat ttttgtggag gtataacgga attaaaagac ttttatctca
     2521 tttttgatgg catttgctcc cggagataat gtaagccgat aattagtcct gtgttaatcc
     2581 gccaacggca aatacctttc ccggatgatg ttgaaatgat gtatctggtg tccgagaata
     2641 ttaaacccca tggccaaaac cgacatctgc gtcttattga atttgcagtc tgaccggaga
     2701 atccgttcgt caaagttatt gaacatccct ttcgtcgcca tccgcactgc acgtaactca
     2761 tcgagtaatt ggtcgatgga taagtcgtcg gcattggcat ggtctgccat aagattgtga
     2821 tcgagtcccg gcggtatagt gccctcattt ctgacagaca gaagtaatct gtaacaccag
     2881 atacgctccc agtctgtgag gtgctggatg attgtgtgcg ttgaccattt tccttccgca
     2941 taagtccgta ggccgatacg cttcaacctg tcaacatcca gggtgtttat ttcctttata
     3001 cttctttcaa aggcatcatt cagttcaatg tcataattga gcaatatgta gtagtcataa
     3061 tattcgggca attccctgat gtctgatttt ttcatctttt taaaggcttt taatctgttg
     3121 tgaatgttta tggagcatga caataaggtt ctgatatcat gcactcagca cttattgttc
     3181 atggctttca aagatatggc atggggatgg ttcaaccaag ttttttacaa tgactcccgg
     3241 caatgggaaa ggcgatcaaa aaaagcgcaa tgttcagcca atcctgctga aataaacctt
     3301 tgacagctat acttctctga cgggatctct tgtttacaaa aaaattagcc ttatcatgtt
     3361 gtcaaaatat tgaataccgg aatgcttcaa ggttataact cccggattat tcagtaaaaa
     3421 atcgtcacca agtccacctt ttcctattgt aagttcatta ctttagattg accacaccta
     3481 aagctgtcac tcaatctccg gaatgccggg actattaata aaagtatcaa ttgcaacctc
     3541 atttcactgc tggtgattaa atcctaattg ttgcaattgg ctatgtttag gttgtttaaa
     3601 atttcagagg cttgttccaa atgccttcgc tcgtgggcaa caatgatgtc aaaggcagtt
     3661 tccagcttat acacaatatt tctgtttgcc ggggatgaaa tgatagttcc tttttcaatt
     3721 aattcgttgg atgtctcgat ttgatgtttc aattcatttt gatggttttt aaaactttga
     3781 aggatatctc ccttgacttg actaaggtcc ggctcccaaa tcggaaacgt tttcattcgt
     3841 ttctttctgt ccggattcac ggcgcgaaga acagtcttcc ctataaatga aaccataaaa
     3901 tcaagttttg caatgaaggg tggtttataa gcgccttctt tcaaagaagc caaaacagga
     3961 aaataggttt cgtttacaac cattaaatgg tcaatattct gggcaatact ccacgtccgg
     4021 gcattgggtt tccagttcaa ttccttttct cccaaatgac caaatttgtc attaaacgac
     4081 tgtgtgataa tatctatttc ggctaaccaa cccttcacca taaaataagt ctgttttatt
     4141 cttttgtaaa tgaaaatatc cgtccgcctt gcttgacaaa aaccatttcc taattgggat
     4201 tgagcgttat aaaaaacggc atttaattcc cttgtttagt tccaacggca acaaataatt
     4261 ttccaccaac cttattttct tttaccggta aatattcctg gtgaaagtta actgttttgg
     4321 tttgtaaggt ctgccatata ttgcggttaa gatcggcctt cgctattttt tcaactaacc
     4381 aggtattaaa atttcttttt tcggcaaata cgtcaacgac tactattttt ccaccctcat
     4441 taagcaatct tagtgcattg tctaagacca tttcccaatc aggaattacc gatagaccaa
     4501 gtacgcaaat aactctgtca aagcttaatt ttttgttggt gaattgctca atcgaactta
     4561 tattcaacga tcgagcgtca gcttgaaata agaagacatt ttcccaatta ttaccttcaa
     4621 ttattttttg tcctttttta agcatcccgc gagaatagtc tgtcccgtat agagtaactt
     4681 tgtcgttggc ggctattata tgcttgaagt tggctcctgt tccgcacgca atatcaatta
     4741 cgatctgatt atccttgaga tccaatagct cgacagctct ttttcgacta ctgtaatata
     4801 atttctctaa agagctatcg taaaaaccag aaaaaaagtc ataccatttc atattaattt
     4861 cttttagtct tccgttcggc tatgcgtcca taaccaggca tctccaattt catggtcttg
     4921 aactatttcg gtaaacacaa aatggttgtt ctctaaaatt tttgttgatg cattgtgttc
     4981 gggtgctgtc tttgctgtta ttattatgcc cgggtctgtc ttataggcaa tatttaaaag
     5041 ctctctgcat gcaaatgaag caatcccctg tccttcaaac gctttaaatg tccaatacga
     5101 aacttcaacc ttaccatcct gtggttgtcc tacgaaactg caagctccaa caattgtgtt
     5161 gtgcctgacc acaaaataac caatccaggg tgtgttgaag ccaatctggg gataaaagtc
     5221 gtcatacatt cgcaacagat gttgacaatc cgttgaagca aatgcttcat ttgttttgtc
     5281 ttcgtttagt gaaattggta ttaatttcat aaagcctgtc tgtgttttct taaatagaat
     5341 atggcgtaaa tggctgtcta taccggattc ggtcggttac catcctaagt atagatttta
     5401 ccactaaact ccggccgata gacaactgct caaatgtagt aattaatttc aaacgatgca
     5461 ctttgccaac catattacca caccgatttt aacggtggtt atacttgtct gtcaggaagt
     5521 cgagtataga ttccgttact ttttcaggtt gttcttcctg tggaaaatga ccacaggtgt
     5581 caagttgaat agtcctcgaa tttgtaaagc cggtttgaaa cttgtcaagg tattgtggtt
     5641 tgattacagg gtctttcatt ccccaaacga acaatgtcgg cttgtcagaa attgccagcc
     5701 gtttcttcca tagttgctca aaccaatcct ggtcgttaag caatgatttg gcaaatgcca
     5761 gcgcaccgtt ccgttgggtt ttgtcggcaa aaggtctggt gtattgcttc aataaatggc
     5821 cggaaatttt gtgatcgcca aacgatttgg gtaaaataaa ttttggcgaa aagttcagat
     5881 agcgatacaa aaaggggagc aaggggcttt tcaatatttt actcatcttt ataaagtcgg
     5941 ggtcggtttc actgctccac aaccaggagt tgagaataac caggcgcttt attctttccg
     6001 ggtgttgaat cgcaaaatta agacctatcg ggccgccgaa atcgtgaacg acaagagtca
     6061 cgctgtttat ttgtttttca agcacaaacc gttcaagggt cctgctgtga ttttgggtgg
     6121 tatagtcgta atgttcgggt ttgtccgaaa gtccaaaacc gatgtggtca atcgcaatgc
     6181 acctgtagtt ggactttaat tttttaataa tgtttcgata atcaaaactc cacgatggcg
     6241 ttccgtgcac aaacaatatc gtatctccct gtccttcgtc aaggtagtgg agcttgtgac
     6301 cgttgatgtc aaagtatttt gaagtaaacg ggtactctgt agtatcaagc caatgatgtt
     6361 ccataacaat ttttaaacaa agatcacatt tatcgggaaa gcaaatcttg gtctatacca
     6421 acatcagaat tttacgctgt tgaatagctt gctgaaattg gttgggtcga ttcccaaata
     6481 agatgcgata tatttgtgtg gcacaaactc aagtaagtgt gaacttcgtt ggcaaaatgt
     6541 tttgtagcgt tcttcaatgg tcatactatg aagttctata tggcggttaa tcattcccgc
     6601 taaaaccgct tctgtcattc gcctgaaaag tcgttcaatc tgttgcgatt gatcgaaaac
     6661 tttttgcaat tcatcatagg taatgtattc caattcagtt tttgtcaggc aggtcaaaaa
     6721 atattttgag ggcatctgaa aagaaaatga ttccggtatt gcacacaatc ccggagggta
     6781 ggtaaaagca ataacttgtg ttttattgtc ggcgtcgaag taagacatct gcacgccgct
     6841 ctttacaaaa tagagctctc gctggacctg gcccggaacg gttatacagt cgcctttttt
     6901 aaaagacttt gttttcaggt tgtcacccag caaattatat tcagccatag ttatggcatg
     6961 aaaatggttg aaaaaattgt atagctccat aaaattaaca agtattcatc tacatgaatt
     7021 tcttagctac aaaaataaag gcacgaatga taatggcatg aaaaaagatg cacttagacg
     7081 tagcttacct atcttcttac attgactgac aaaagtcagc catccatccg gcaaaaaaga
     7141 tactaatcca cctaagagaa atacagccca tcatccagtc tcgctgaatg gcaaatattc
     7201 aaaagctgcc cggcttagtg gcttaatgtg gaagattttt agcgggcttt tgtatattcg
     7261 gcttaatgtt gtgcgctgcc tttcctttat tgtcaatgca ctgctgtaac atttactttt
     7321 tttattcctt ttaagtttag tttaatgttc ctgtcttttg ctttgttatc gtattggctg
     7381 ataatttcca atatgtcgta gtcaataaaa ttacattcgc ttccatcaat tgtcaatttg
     7441 cttttttcag gcacatcgtc taatgccttt cgtaagttta ctttattgag aaaagttacg
     7501 ttgctgttga gtttaattaa gaaggtgtca attccatttt caactgtttc agttatttta
     7561 tattcctctt tgaaattgtt gcgaataata taatagaagg ataaaacaag cccaatagtc
     7621 actccaatca gtaagtcagt caataggata gcaaaaatag taatcataaa tgggagaaac
     7681 tgttttaatc ccaaactcca catattgcga aatagctttg gttttgccag gttataaccc
     7741 gttacaatga gaattgctga aagcgaagcg taaggaattt tatttaataa gaatggaata
     7801 aataacacag acaatagtaa aaacaacccg tgtgtaaatg cagaaagctt tgtccttgct
     7861 ccagcgtcaa cattggctga accccttaca attaccgcag taatgggtat ggctcctaag
     7921 aagccacaag ccatattccc tatgccttgt gcaaccaact cacgatttac aggggttatc
     7981 ctgtttcgcc tgtctaattt atcaatggct tctatgcaaa gtagtgtttc aagtgtcgca
     8041 agcattccta ttgtcaaacc gtctttcaaa agtccactgg atgtaaaaag ttttgtaaag
     8101 tcaggaaatg aaatgtttgc aaaaacatcg gatggaatat tgaccaattg tgttggtttg
     8161 agtgaaagct cagaagatag gctttcaaat gcagtatttg ttagaatccc aattaccaca
     8221 atgaccagtg gggcaggaag tattttaagt ttattggcga aaggtcgttg aagcaatccc
     8281 attacaataa tggaaatacc tgttattaat attgcccctc ttgtaatgtg ctgattgaaa
     8341 tcatttatgt ttccaacaaa ttggtcagta gagaatagtt ttaaaatacc acctgcccaa
     8401 aagtcgggtt ggtcataacc taatgccaca gggatttgtt tagaaataag aataatacca
     8461 atagctgcta acattccttt aataactgcc gaaggaaaat aatttgcaat gacaccaagt
     8521 ttcaacagtc caagtattag ttgaaacaat cctgcaataa ttacggcaag aagaaatatt
     8581 ttatagtcgc catgcgaaac gatggatgct gctactacgg ttgtcaaacc cgcagcaggt
     8641 cctgaaaccg ctaagtgcga accgctaatg agggaaacaa ctaagccgcc aataatacct
     8701 gaaagaattc ctgaatagag tggtgctccc gaagctaaag caattcctaa gcaaagcgga
     8761 agagcgacaa gaaaaactga aagtccggct ggtaagtcat atttgagcca tattattctg
     8821 taatacttta ttgttctttt cacttccttg tcaaattatt tttgtctgtc aacgatagag
     8881 cttaaatggt tgagatgtcg tcataaagtt actcacaaca tctgcatata cgtcatatgg
     8941 cgtatatttt ccatataaaa gtaagctttc cctcaaaact ccaatctatc
//
LOCUS       DAOQQN010000084         8963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2963_length_8963_cov_6.228110, whole genome shotgun sequence.
ACCESSION   DAOQQN010000084 DAOQQN010000000
VERSION     DAOQQN010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2963_length_8963_cov_6.228110"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..810)
                     /locus_tag="PLG24_04615"
     CDS             complement(94..810)
                     /locus_tag="PLG24_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HMZ24203.1"
                     /translation="MNYNIEISGVRVRVEVRYGPTRNIRLFYRNNTARLTVPKGLREK
                     DLIRAKGKLHEWLINLATAKPHLFVSDEYRALTKEKIILLGQCYHLKFKATQSNRITI
                     HRKEDTLAMEGTDEFFRDPEQMSDFIPRLFNKVFARDIEQRVRAINDQTLRANIGRIS
                     LKSVVSRWGSCSPDNNIMISNRLLLAPTEILDHVIIHELAHTVHRDHSRQFWRVVAQY
                     DPAMKEKNDWLKQNGHTLNY"
     gene            complement(829..1335)
                     /locus_tag="PLG24_04620"
     CDS             complement(829..1335)
                     /locus_tag="PLG24_04620"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HMZ24204.1"
                     /translation="MKKLILFFLLASTTLYGQDASGEYRHEIKLNGLNLVLGNPELDY
                     EYLINSNSGAGLYANANIDRNHIWPFVYMAGAYYRLYLGGKHANGFFFEGGFNAIGFD
                     DTEFGKNKVSNGWGPSFGIGGKFLIKKTIVMEFFGGLGRNFKATDEYNNYDIPVFPRW
                     GISIGKRF"
     gene            complement(1681..2493)
                     /locus_tag="PLG24_04625"
     CDS             complement(1681..2493)
                     /locus_tag="PLG24_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HMZ24205.1"
                     /translation="MKTILVPTDFSETAEKALNAAVHIGGFQQTDLVLMHTSLPTEGV
                     NSNVYAAIYIHEYLETKRKNLNLLADKVRETGYKGKVKTDNKVDFTVPSIVNAAEEFD
                     ADLVVMGSTGSSGLAGVFLGSIAGGVLSKIERPLFLITKDFTLPEGGDLLIGTDYKTR
                     PDKNSLEILRFFKEQLNFSLNLVHISHGEEVSINKEAFVNNHFDGMIDSHVDVPGAKV
                     EGALMKIKEKYQNAVLCTITRKRSWFESLFSHSVSRSLTHYSDIPLLCLQSE"
     gene            complement(2630..3544)
                     /locus_tag="PLG24_04630"
     CDS             complement(2630..3544)
                     /locus_tag="PLG24_04630"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HMZ24206.1"
                     /translation="MDYRTVLNEIYSVGKVMEGLGNVATYIPELSNVDPDKFGIYLLT
                     LDRQGYGIGDDKERFSIQSISKIFTVSMAIRILGESVWKRVGVEPSGSSFNSMVQLEY
                     EKGYPRNPLINPGALVIADMLISNLANPKEDLLDFVRMVSGYESITFDEKVAESEKRT
                     GFRNAAMAYMLKAFNNLDNDIDTVLDFYFHQCSITMSCKELASAFFYFANKGYTYDNS
                     IQILTESKVKRLNALMQTCGFYDESGEFAYKVGLPGKSGVGGGIIAIYPGRYTVATWS
                     PRLNSKGNSVRGMKALEMLTTFTTESIF"
     gene            3866..4171
                     /locus_tag="PLG24_04635"
     CDS             3866..4171
                     /locus_tag="PLG24_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMZ24207.1"
                     /translation="MKEITVEELKAKLDAKEDFVFIDVREPHEYDVFNLGARLIPLGE
                     FLKDVHILDDNKGDEIVIHCRSGMRSSMAVEALVNSGYTNVRNLVGGVLRWQEVFPG"
     gene            4235..5086
                     /locus_tag="PLG24_04640"
     CDS             4235..5086
                     /locus_tag="PLG24_04640"
                     /inference="COORDINATES: protein motif:HMM:NF025007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HMZ24208.1"
                     /translation="MQISKLDKVAGLDRASFIEHFLKPMKPVVFTDLTKDWSALQNWT
                     VENFKSKYGNLKVSVYTNKFEGTDKGFREPDKHMLFGDFLDLLVSSEPCDLRLFLFNI
                     FKYAPELRGDFDLPDITDGFYNDLPSMFFGGKGSKVAMHYGLDLSHVFLNQIYGKERV
                     VLFSPDQSRLIYQLPYSVAGNIDINNPDYDRYPALRNAKGEEVVLNPGESLFIPSGYW
                     HYIEYVEGGYSVSLRANESIRRRMKGAANIATHFIVDKGMNRLLGSKWREIKDRMVIW
                     RAQAMID"
     gene            5187..5924
                     /locus_tag="PLG24_04645"
     CDS             5187..5924
                     /locus_tag="PLG24_04645"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HMZ24209.1"
                     /translation="MHTKLSVNVNKIALVRNSRGADYPNLLEMSLDLEDYGAEGITVH
                     PRPDQRHIRYDDIPILRAHLRTELNIEGFPSEEFIRLVIDNKADQVTLVPDSPEALTS
                     DHGWDFEKHEDLLRAIVPVFKDAGIRVSLFVDPQPDNADKAQAVGADRIELYTGPYAH
                     RFERDGNGGVQAHTDTSRRALNIGLGVNAGHDLNLKNLAFYKRHMPHLQEVSIGHALI
                     VDAMYFGYSNAIQMYKDRLIHPEHYLG"
     gene            6265..>8963
                     /locus_tag="PLG24_04650"
     CDS             6265..>8963
                     /locus_tag="PLG24_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HMZ24210.1"
                     /translation="MKKTMFTLFLLVLGFVVSYGQRTISGMVTDDKSEPLVGASVLIK
                     GTQDGTVTDVDGKFSLRVPSGQATLVVSYTGYVTKEVVLDGVSNTVSVSLETDKRILD
                     EVVVTAYGLERKRNELSVSAQKVTGDEVNVVRSNDFVSALNSKVAGLDIRTGNTMGGS
                     TNVVLRGYKSITGNNQALFVIDGVPAANQTLTSSSTAQGREGYDYGSSAADINPSDIE
                     SITVLKGAAAALYGSRGANGVIIITTKKGKKDRLDVTLNSGLSWGKIDRSTFVTHQDQ
                     YGAGYGPDFYSYNGGSGFLGSDIFSPGVEEPFVPFTEDASYGAKFDPNFLVYDWRSVD
                     PLNPNYKKKSPWVAAPTDVAEFYETAFSSNQSISIEGGGSTSTFKVGYTRNDEKGVMP
                     NSSLYKNMFSIGGSLEASSKFKISSNFNFSNEAATGRQGIGYGNRNPNTMFRQWYQTN
                     VDVSDLKAAYELHEKNATWNWADITAENPIYWDNPYFARYKNYTTDSRNRYFGNIMAT
                     YTIMPGLSLVGRAGGDISFDQIEERNNKSSVDLGSYSVTNRSYKEYNYDVFANYNKEL
                     SEAISLDITAGTNIRRSYLYQISASTNTDLVVDGLYSIANSLQTPTPPSEGYVPVGVD
                     GLFATATIGFNKFLFLDGTFRRDRSTTLPKANNTYNYPSVSLGFIFSEILKQDWLNYG
                     KLRASYTQVGNDAPALSVFDVYDKPTAFGSVPFFSLPARKNNPDLKPERTKSLEFGID
                     LNMFDNRIGLEYTYYDATTFDQIIPVQITAATGFTSRFVNAGSVNNKGHEVILNLTPV
                     RTKDLSWTLTFNFAKNKNEVVELYDENTKQIVLGTFQSGISLVAIPGQPFGVIKGRGF
                     SYHENGEKIVGANGYYTRKTDQILGNINPDWFGGVGSTLRWKN"
BASE COUNT         2594 a         1783 c         1945 g         2641 t
ORIGIN      
        1 ctgttgatgg aatacattgc cgaatgccca caagtaaggg acgtcatgct tatcattgcc
       61 acaaccaccg aaaagttggg aagcaatttg acatcaatag ttcaaagtat gtccgttttg
      121 tttgagccag tcatttttct ctttcatggc aggatcatat tgagcgacta ctcgccaaaa
      181 ctgtcgggag tgatctctat gtaccgtgtg ggccaattcg tgaatgatga catgatccag
      241 gatttctgtt ggtgctaata gtaatcggtt ggaaatcatg atgttgttgt cgggtgagca
      301 agacccccag cgcgacacaa cgcttttaag actgattcgt ccaatgttgg ctctgagggt
      361 ttgatcattg attgctctga cccgttgttc gatgtcacgg gcaaacacct tgttgaacag
      421 ccgaggtatg aaatctgaca tttgctcagg gtctctgaaa aattcgtccg ttccttccat
      481 cgcaagggta tcctctttcc ggtgaatagt gatccggttt gattgagttg ccttgaattt
      541 caaatggtaa cattgtccga gcagaatgat tttttctttc gtcaaagctc tgtattcatc
      601 ggatacaaag agatgcggct tggccgttgc caggttgatc aaccattcat gcagcttgcc
      661 tttagctctt ataaggtctt tttctcgtaa acccttggga acagttagcc ttgcggtatt
      721 gtttctatag aataaacgaa tgttgcgtgt aggcccatat cgcacttcta cccgtactct
      781 gactccactt atttcaatat tataattcaa aacatccggg gttttgggtc agaacctttt
      841 acctattgag atgccccatc tcgggaatac aggaatatca taattgttgt actcatctgt
      901 agctttgaaa ttacgaccca ggcctccaaa aaattccatg acaatcgttt ttttgatcag
      961 gaactttcct ccaattccaa aggatggccc ccatccgttt gacactttat ttttaccaaa
     1021 ttctgtatca tcaaaaccga ttgcgttgaa tcctccttca aagaaaaacc cgttggcatg
     1081 cttacctcct aaatataacc ggtaataggc accggccata taaacaaatg gccagatatg
     1141 attcctgtct atattggcat ttgcatacag acctgcgccg gagttgctgt ttatcagata
     1201 ttcataatcc aattccgggt tgccaagaac aaggttaagc ccatttagct tgatttcatg
     1261 cctatattcc ccggacgcat cctgaccata taaagtagta cttgctaata aaaaaaataa
     1321 aatcagtttt ttcataccgg atttctcaat ttgattacac gagacgaaga tatgtatcgt
     1381 gcttaattaa actattttat tatgttttat ttaactaaag tttctgacgt ccccatatct
     1441 ttattgaaag gaagttcatg ggatattggc acactgatat ttattaagta tttcagaacc
     1501 tttagtggta tttccggcgc cacaatccaa tcaccggagg ccaaatgctt caatttatat
     1561 ctcagttgct tcagttgagc aaccattata caattcgtac tttggtgtaa gtcgaaaacc
     1621 aaagatggat aaaaaatgct atggcttacc ttgatcgtga acattgtatt ccggacctgc
     1681 ctattctgat tgcaagcaaa gcaatggaat atcggaatag tgcgtaagcg agcgggatac
     1741 actatgtgaa aacaagctct caaaccagct tctttttctt gtaatggtgc acaatacagc
     1801 attttggtat ttttccttga ttttcatcaa tgctccttcc acctttgccc ccggaacatc
     1861 aacatgggaa tcgatcattc catcaaaatg attgttgaca aatgcctcct tgttgattga
     1921 aacctcttcc ccatgggaaa tatggacaag attcaatgaa aaattgagtt gttctttgaa
     1981 gaaacggagg atttccagcg aattcttgtc aggtcgtgtc ttataatctg taccgatcaa
     2041 caagtctccc ccttccggaa gggtaaagtc ttttgtaatg aggaagagcg gcctttctat
     2101 ttttgacagc acaccaccgg ctatacttcc aagaaaaaca cctgccaaac cacttgagcc
     2161 tgttgatccc atcaccacaa gatcggcgtc aaactcttct gcggcattga ctatggaggg
     2221 aaccgtaaaa tcaaccttat tatctgtctt gaccttccct ttatatcctg tttcgcgaac
     2281 cttatccgcc aataaattca aattctttcg cttggtttca agatattcat ggatataaat
     2341 ggctgcatat acattactat tgaccccttc tgtaggaaga gaggtgtgca tcaagaccaa
     2401 atctgtttgc tgaaaaccac caatatggac tgctgcattt aaggcctttt cggccgtttc
     2461 actgaaatct gtcggtacaa gtattgtctt catgagattg agattaataa ataattgttt
     2521 gttgttctac aaatataacc taatgcactt taaaatagtt atcaaatctt gtcttttttg
     2581 actgaaatca ttgaatttcc ataggcttta ctgcagaaga cctccggatt taaaaaatag
     2641 attcggtcgt gaaggttgtc agcatttcca gtgccttcat tcctcgaacg gaattgcctt
     2701 tgctgttgag gcgcggactc caggttgcaa ctgtatacct gccggggtat atggcgatga
     2761 ttccgcctcc gacgccgctc tttcccggca accccacctt gtaggcaaat tcacctgatt
     2821 cgtcatagaa accacacgtc tgcatcaggg cattgagtct tttgaccttg ctttccgtca
     2881 ggatctgaat agaattgtca taggtatatc ctttattggc aaagtaaaag aaggcgctgg
     2941 ccagttcttt acaagacatg gtgatactgc actgatgaaa atagaaatcc agcactgtat
     3001 ctatatcgtt atccagattg ttgaaggctt tcaacatgta cgccattgct gcattccgga
     3061 atcctgtcct tttttcggac tctgccactt tctcgtcaaa tgttatggat tcatagccgg
     3121 agaccattcg cacaaagtca agtaaatctt cctttgggtt agccaggttg cttatcaaca
     3181 tatctgcaat cacaagtgca ccgggattga tcagcggatt gcgcgggtac cctttttcgt
     3241 attcgagctg aaccatcgaa ttgaatgaac ttcccgaagg ctctaccccg actcgtttcc
     3301 aaacagattc ccccagaatt ctgattgcca tcgacactgt aaatattttg gaaatactct
     3361 gaatggaaaa tctctcctta tcatccccga taccataacc ttggcggtcc aatgtcaaaa
     3421 gataaatacc aaacttatcc ggatcgacat tgctaagctc cgggatgtag gttgccacat
     3481 tgccgaggcc ttccattacc ttgcccacgg agtaaatttc gtttaataca gtccggtagt
     3541 ccatttttta taaaaattta tcaaatataa cccaaaagtg aagaatgctg attctaaaat
     3601 gccggatctg actttgacct tgttagacaa ttcactttct gatatagcgt attgaaggat
     3661 tgatcggaag ccgttcggat caggtatttg acagggaaaa ggacggtatg gattaagccg
     3721 gccaaaggtc gggactgatg attatcaata ttttattagt ccaattattg cactaaatgt
     3781 aaatttgttt gacaaaaaga tggtgccgga tgcttccatt gcaataccac accatagaat
     3841 aagcaacatg atcaattatt agataatgaa ggagataaca gtagaagaac tcaaggcgaa
     3901 gttggatgcc aaagaggatt ttgtctttat tgatgttcgg gagccacatg aatatgatgt
     3961 gtttaacctg ggagccagat tgattccctt gggagaattt ctcaaggatg tccatatact
     4021 ggatgataac aagggcgatg agattgtcat tcattgtagg agcgggatgc ggagcagtat
     4081 ggccgtagaa gctctggtca actccggata cacaaatgtc cgaaacctgg ttggtggtgt
     4141 gttaagatgg caggaagtgt ttccggggta aactttatgg cggataattt aagctaaggt
     4201 tttcactatt ttgaaaaaag atgagaatcg gataatgcag atttcaaaat tggataaagt
     4261 ggccggactg gaccgtgcct cgttcattga acattttcta aagccaatga agcctgtggt
     4321 atttacggat ctaaccaaag attggtcagc attgcaaaat tggacagttg agaatttcaa
     4381 atcaaaatat gggaatctca aagtttctgt ttataccaac aaatttgaag gtactgacaa
     4441 gggttttagg gagcctgaca agcacatgct ttttggggac tttctcgatt tgctggtgag
     4501 ctctgaaccc tgcgacctgc gactattttt attcaacatc tttaaatacg cgccggagct
     4561 tagaggagat ttcgacctgc cggatattac ggatgggttt tacaatgact taccatcgat
     4621 gtttttcgga gggaagggta gcaaagtcgc catgcattat ggtcttgacc tgagtcatgt
     4681 atttctcaat caaatttacg ggaaggaaag agtagtacta ttttcaccgg accaaagtcg
     4741 gttgatctac caattgccat attcagtagc cggcaatata gatatcaaca atccggacta
     4801 tgataggtat cctgctttaa gaaatgctaa gggagaagag gtggttttaa atccgggcga
     4861 atcccttttc atcccatctg gatattggca ttatattgaa tatgtggaag gaggatatag
     4921 tgtttcctta cgagccaatg aaagcatcag aagacggatg aagggagcgg ctaatattgc
     4981 cactcacttt atcgtggata aggggatgaa caggcttctt ggatctaaat ggcgggagat
     5041 aaaggatcgg atggtcattt ggagggctca ggccatgata gattgatatg gagcatacac
     5101 tgtcagagtt agctttggta gtgactcaat tacaagatag gtggaaattg accattttca
     5161 ttttttaata actgaataat taagtgatgc acaccaaatt aagtgtaaat gtcaataaaa
     5221 tagctcttgt aaggaattca agaggtgcag attatcccaa tctgctcgaa atgagtttgg
     5281 atcttgagga ttatggggct gaaggaatca cggtgcaccc gaggccggat caacgccata
     5341 ttagatatga tgacattcct attctgaggg cccatcttcg gacggaattg aatattgaag
     5401 gcttcccttc agaggagttt atccgacttg tcattgataa taaagccgat caggtgacat
     5461 tggtgccaga ttcgccggaa gcattgacct cagatcatgg gtgggatttt gaaaagcacg
     5521 aagatttgct tagagccata gttccggtat tcaaggatgc cggcatcaga gtctccttat
     5581 tcgttgatcc gcaaccggac aatgctgaca aggcacaggc ggttggcgcg gataggattg
     5641 aattatatac cgggccttac gcgcatcggt ttgaaaggga cggcaatggt ggtgtgcaag
     5701 cccatacaga cacttcccga agagcattga atatcggttt aggggtcaat gcaggtcatg
     5761 acctcaacct gaagaatctt gctttctaca agcgacatat gcctcatctt caagaggtat
     5821 ctatcggcca tgccctgatt gtcgatgcta tgtattttgg ctattcaaat gctatacaga
     5881 tgtataagga tcggctcatt catcccgaac attatttggg ataaggacgc cacacataat
     5941 aggtctcgga ccactgctgc cgagctaatg ccggtcggca attgcacggt agctattcat
     6001 ttcattgaat ccatatatca gcttgttaaa taaacattcc ttaaaatcac gacttgatat
     6061 tgtattttta acacaaagta ggccaaatcc gttttgtcat atgaaagaaa aacagtattt
     6121 tcaattgttt ttcaattgtt tttagattta ttgtaataaa tttgttaaaa aaatcccaaa
     6181 aaaagtatgc acgctatgta ttaagtttat tacatttggt attcatattg taattcatta
     6241 attaaataat caaaaagttc aggcatgaaa aaaacaatgt ttacgctatt tttgcttgtc
     6301 cttggttttg ttgtttcata tggacagcga accatttcag gtatggttac ggatgataaa
     6361 tccgagccgc ttgtaggggc aagtgttttg attaaaggaa ctcaggatgg gacagttacc
     6421 gatgtcgatg gtaagtttag tcttcgggta ccttccggtc aagcaaccct ggtagtttcc
     6481 tacacgggat atgtaactaa ggaagtggtg ttggatggcg tatccaatac tgttagtgta
     6541 tctttggaaa ccgataaaag aatattggat gaagtggttg tgaccgcata cggtttagaa
     6601 agaaagagaa atgaactttc tgtttcagct caaaaagtaa ccggtgacga agttaatgtc
     6661 gtcagaagta atgactttgt aagtgcactt aacagtaaag tagccggtct tgacattaga
     6721 accggtaaca ccatgggagg ttcaaccaac gtggtactca ggggttataa gtccattaca
     6781 ggtaataacc aggcgctgtt tgttatagac ggtgttcctg ctgccaacca gactttgacc
     6841 agttcttcca cagctcaggg aagagaaggg tatgactatg gttccagtgc tgctgacatc
     6901 aatccaagtg atatcgaaag tataacagtt ttgaaaggtg ctgctgctgc gctttacggt
     6961 agccgtggag cgaatggtgt aattatcatc acaaccaaga aaggaaagaa agacaggctt
     7021 gatgttactt tgaattccgg tttgtcttgg ggtaaaatcg accgttctac ctttgtgaca
     7081 catcaggatc agtatggtgc aggttatggt ccggatttct attcttataa tggcggatca
     7141 ggattcctcg gatcagatat tttctctccc ggcgtggaag aaccgtttgt tccatttacg
     7201 gaagatgcat catacggtgc caagttcgat cctaacttct tggtttatga ctggaggtca
     7261 gtcgatcctt tgaacccgaa ttataagaaa aagtctccat gggtagccgc accaacggat
     7321 gtagctgagt tttatgagac tgcattttcg agcaatcaga gtataagtat tgagggtggt
     7381 ggtagtacat caactttcaa agttggctat acacgcaatg atgaaaaagg tgtcatgcct
     7441 aacagcagcc tttataaaaa catgtttagt attggaggat cattggaggc aagttccaag
     7501 tttaagatca gttccaattt caacttttca aatgaagctg caaccggtcg tcagggtata
     7561 ggatatggta acagaaaccc aaacaccatg ttccgtcagt ggtaccaaac caacgtagat
     7621 gtatctgact tgaaagcagc atatgaattg catgaaaaga atgctacctg gaactgggca
     7681 gatattacag ccgagaaccc catttactgg gataacccat actttgcaag gtataaaaat
     7741 tatactacgg attcaagaaa cagatatttt ggaaacatca tggcgacata caccattatg
     7801 ccgggccttt cattggttgg tagggcaggt ggtgatattt catttgacca gatcgaagag
     7861 agaaacaata aatcttccgt ggatcttgga tcttacagtg taaccaacag aagctataag
     7921 gaatacaact atgatgtatt tgccaattac aacaaggaac tttcagaggc catctctctg
     7981 gacatcacag caggtaccaa cataagaaga tcttatctgt atcagatctc agcaagcacc
     8041 aatacagacc tggtcgttga tggtttgtat tctattgcca actcacttca gacaccgact
     8101 ccaccatcag aaggatatgt tcctgttggt gttgatggtt tgtttgctac tgcgactatt
     8161 gggttcaata agtttttgtt cttggatgga acatttagaa gggaccgttc aactacattg
     8221 ccaaaagcta acaatacata caactatcct tctgtatcct taggatttat attctctgaa
     8281 atattaaaac aggattggct caactatggt aagttaagag cttcctatac tcaggtcggt
     8341 aatgatgctc cggcattgag tgtattcgat gtgtatgaca agcctacagc attcggatct
     8401 gtaccattct tctccttgcc ggctcgtaag aacaatcctg atctgaagcc tgagagaaca
     8461 aaatcattgg aattcggtat agacctgaac atgtttgaca acagaatcgg attggaatat
     8521 acatactatg atgcaacaac atttgaccag attatccctg ttcagattac tgctgccaca
     8581 ggatttactt caaggtttgt aaatgcagga tcagtaaaca acaagggaca tgaagtgatt
     8641 ctaaacctga caccggtgag aaccaaggat ttaagttgga cattgacctt caactttgca
     8701 aagaataaga acgaagttgt tgaactatat gatgaaaata ccaagcagat tgtactcggt
     8761 acattccaga gcggtatcag cttagttgct atcccgggac agccatttgg tgtaatcaag
     8821 ggtagaggat tctcatacca tgaaaatggt gagaagatcg tcggtgcaaa tggatattat
     8881 actagaaaaa ctgaccagat tcttggtaac atcaaccctg actggtttgg cggcgtaggt
     8941 tccacactga gatggaagaa tat
//
LOCUS       DAOQQN010000085         8956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2969_length_8956_cov_5.638018, whole genome shotgun sequence.
ACCESSION   DAOQQN010000085 DAOQQN010000000
VERSION     DAOQQN010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8956
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2969_length_8956_cov_5.638018"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PLG24_04655"
     CDS             complement(<1..558)
                     /locus_tag="PLG24_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol 3-phosphate dehydrogenase"
                     /protein_id="HMZ24211.1"
                     /translation="MRVSPLAFKEPVGVIGSGSFAVTIAHLLTQNVNVLLYARRPEVV
                     DEINTMRRMYGHDLHERISATNDISRIGKECSVIFPIVPSEFFRNTIRQLAQFLSPRH
                     ILIHGTKGFDIIGDFDYDLEGFSRDNIRTMSEVIQEETSVIRIGALSGPNLYREILDG
                     QPAATVVASKYDEVVKMCQKVLDGPD"
     gene            complement(586..2433)
                     /gene="yidC"
                     /locus_tag="PLG24_04660"
     CDS             complement(586..2433)
                     /gene="yidC"
                     /locus_tag="PLG24_04660"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HMZ24212.1"
                     /translation="MDRKSIIGFILLAVLFYVWMTLNQNNNKKIQAEKKLQDSVMLSK
                     ITQQKADSLKALEALSPRNDSAGIKISDTSSIVKRQPPAEVTLENDLIALTVSNVGAK
                     IKQIHLKQYKTSAEDAKGNEVREPLLLQEAPENEMVYILPLANGSKLETVSLPSTVEK
                     SGDKIILKTELPNQAFLEQSYQFDTAAYQIDYQAKIIGDPGLDKSKPITVRWVNNLTR
                     LEKNYQYERTYSTLYFKIAGESSDYTSYSKNTTVKLKPGQQLEWLSAVNQFFNSSWVP
                     EKPFDNAELTITQGEGEDKYLKKTEATVTFPIEFSSGQSFGMKVYAGPNEFNRLREYN
                     NSLEDIIPFGSSIMGSINKWVVRPLFNFLGTFISSKGLVILMLTLLVKLALWPLTYKM
                     IYSQSKMSSLKPHLDKLKAKYGDDSSKIQMETMNLYREYGVNPLGGCLPMLLQMPIWF
                     ALYRFFPAAIEFRQAPFLWATDLSSYDVIAKFSFNIPMIGHHLSLFTILWTVTTLLYT
                     WYNFKKIDTTSTAANPALKYMQYIMPVFFMAFFNSFASGLTCYLVFSNIINIAQTLIT
                     KNYLISDEKIQKELHAYKQKPKKKGGFSDRLGEMLKEQQKIAEQRNKKK"
     gene            complement(2465..3043)
                     /gene="dcd"
                     /locus_tag="PLG24_04665"
     CDS             complement(2465..3043)
                     /gene="dcd"
                     /locus_tag="PLG24_04665"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HMZ24213.1"
                     /translation="MGDWLWLISLFIDKMILSDKQILQGIDDGTIVIEPFELSCLGTN
                     SYDVHLGKYLATYVDEVLDARKHNPIELFEIPEEGFVLMPEKLYLGITLEYTETHATV
                     PFLEGKSSVGRLGIDIHATAGKGDVGFCNHWTLEISVSQPVRVYAGMPVGQLIYFKVE
                     GDIDNYYNKKIGAKYNVRSDKPMESMMWKNKF"
     gene            complement(3066..3512)
                     /gene="rplI"
                     /locus_tag="PLG24_04670"
     CDS             complement(3066..3512)
                     /gene="rplI"
                     /locus_tag="PLG24_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HMZ24214.1"
                     /translation="MDIILLKDIDKVGNKHEIVKVKDGYGRNYLIPQGFAVVANAGNR
                     KMLAELVKQEDRKEAKRVDEYKEIAAKLTDVTLKIGAKAGTSGKIFGSVTNIQISNAL
                     KDQVGVEIERKKIILEDDIKTLGTYSAKLNLHKDVQSTVAFEIIEE"
     gene            complement(3535..3801)
                     /gene="rpsR"
                     /locus_tag="PLG24_04675"
     CDS             complement(3535..3801)
                     /gene="rpsR"
                     /locus_tag="PLG24_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HMZ24215.1"
                     /translation="MASRDDIKFLSNPNLGQNRKKYCRFRKYGIKHIDYKDPDFLLQF
                     INEQGKILPRRISGNSLKYQRKMATAIKRARHLAMLPYVADLLK"
     gene            complement(3833..4276)
                     /gene="rpsF"
                     /locus_tag="PLG24_04680"
     CDS             complement(3833..4276)
                     /gene="rpsF"
                     /locus_tag="PLG24_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HMZ24216.1"
                     /translation="MKQFEVTFIVDPVLSGDELKSTVGTYTDMLKNEGCKIVAIDELG
                     LRQLAYDIKKRSSGVYYTIEFQAETGAIIAKLELALRRDERIMRFLTISLDKFGIKYN
                     DDKRNGKIGKVKEKVKAKEEQPEAKERKETQAPVVEENQVESTEE"
     gene            complement(4410..5750)
                     /gene="hisS"
                     /locus_tag="PLG24_04685"
     CDS             complement(4410..5750)
                     /gene="hisS"
                     /locus_tag="PLG24_04685"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HMZ24217.1"
                     /translation="MKPALPKGTRDFLPGEIKKRNYLFNIIREKFALYGYDAIETPAM
                     EALETLAGNYGEEGDKLLFKVLNNGDFLKDVEDSFLTDKDSFGIIRHISKRGLRYDLT
                     VPFARYIVMNRQHIKFPFKRSAIQPVWRADKPQKGRYQEFYQCDADVAGSNSLYYEAE
                     LIRLYDDVFTALNLPVNIRWNNRKILFGISQTVGLSEHFHEVTVIIDKLDKIGKEGIE
                     RELIGKGFPVQSIRDLLKLIALTNLEELEEHMNSETGTLGIVEMREVLGHLSEAPLEN
                     HLQFDATLARGLGYYTGCIFEVSALNANMGSIGGGGRYDNLTANFGLKDVSGVGISFG
                     IERIYDVMEEKKLFPEQISRSKQVLIVCLDNESFGYAVGVTNQLRVQGVSAELYPVVA
                     KLQKQMSYANDLDFAFVCLIGEAERTAGKISLKNMMTGVQDIFSFHEVVARIGQ"
     gene            5878..6432
                     /locus_tag="PLG24_04690"
     CDS             5878..6432
                     /locus_tag="PLG24_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMZ24218.1"
                     /translation="MDIPISMDFRERDLISACIRREEWAIRKVYEDHYSLMMSICLRF
                     AANESDAMDLLHDGYIKVINNFEKYVPNTSFPSWLHRLIVNNCIDHFRKMSRRRTEDI
                     EQIYHTAIIDPDAVSKCTEKEILNAIQKLSPTYRTVFVLAAIEGYSHKEIATELGITE
                     STSRSNLVKARLRLQELLKEKIGM"
     gene            6432..7904
                     /locus_tag="PLG24_04695"
     CDS             6432..7904
                     /locus_tag="PLG24_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24219.1"
                     /translation="MLQEKEFDRIIKHAMDGMQIKLPTDAWDQFSQKTGCKNLSSRTR
                     FDKCGNVGLSHYYSAIGPSVGAQFSENQQEFQILAEEEFDAAIKKKLTGNSINQTPKT
                     SILNSILFRFNLHNHRLFIYKCLEACTLALFFWIFTWHIYYEQNSSQQEMPMAVISEE
                     SKTSNHTLNIAAHNQLESTSYSREPIRNHTGNKVTKDLNQKQIPAIQYPTGLYPSLQA
                     FTKETDHIATNQYSSDHPSTAHEDFHGTNWSTPEITPIKKIEPGRIEISKKEITISPI
                     LPQHKKESKNKSLWLGLNASWIFDKMWAPGYIANTRAIITNRFQSKGLGAEVEFGIAK
                     AQIATGINYSYKKFDTGTKVHNEGHYINIPLTVKKEFGRSEQIIPYCKIGPEITLAAT
                     TSYTPIIPEKPDNKEAFAPLFVPDKQVSDGILKGDFKKNSYFGLHIALGIEVLIKQSS
                     KIFIEAAHQVNPFEKGVGDNNQRLSYTTVNLGFKKNLYEN"
     gene            8132..>8956
                     /locus_tag="PLG24_04700"
     CDS             8132..>8956
                     /locus_tag="PLG24_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24220.1"
                     /translation="MKKLFGFWNLMLLVGASLFVVSCNPDDNPTSSKGPTVTETHTFD
                     ATKDQDPLLPIIITVKGVKGDADLKSLTVKENGTNLDVDRFLVDDNDPSSAAILLLGA
                     DKQGFTKVFEIDPVAKTGAYKYEFVLTDEKNNTNTVALEVNIKTVATITSEKMYNYLG
                     PLAGGLDLLTGKAVVKGADVNNEWATAHIRDNGNDVVGNTYPWKGEFIPWKGSVVRKT
                     DNSNWDLTVSPGQISSLFDNGLTDLATVKMKVGDVYVVKNGDHYFMIKVKEVSDDGS"
BASE COUNT         2796 a         1840 c         1591 g         2729 t
ORIGIN      
        1 gtcagggcca tcaaggactt tttgacacat cttgaccact tcatcatatt tagaggctac
       61 gacagttgcg gcaggctgtc catccaatat ctcccgatac aggttgggac ccgacaaggc
      121 accgatcctt atcacactgg tttcttcctg aataacctca ctcatggtcc ggatattatc
      181 tctggaaaaa ccttctaagt cataatcaaa atcaccaatg atatcgaaac cttttgtacc
      241 atggattagg atatggcgtg gactcagaaa ttgagccaat tgtcggatcg tatttctaaa
      301 aaactcagag ggaacaattg ggaaaattac agagcactcc ttccctattc tgctgatatc
      361 atttgtcgcc gaaatccgtt catgcaggtc atgaccatac attctacgca tagtgttgat
      421 ttcatccacc acttccgggc gccttgcata aaggagcaca ttgacatttt gagtcaacaa
      481 atgtgcaatt gtgacagcaa atgatcccga accaataaca ccaacgggct ccttaaaagc
      541 caagggagat acacgcattt tactcaatca aagaagtaaa tataactact tctttttatt
      601 tctctgctca gcaatttttt gttgttcctt aagcatttca cccaaacgat ctgaaaatcc
      661 acctttcttt ttgggttttt gcttatacgc atgtaattcc ttttgaattt tctcatcact
      721 aatcaaataa ttcttagtaa taagtgtctg agcgatatta atgatgttac taaaaacaag
      781 ataacatgtc aatccggagg caaagctgtt gaaaaatgcc ataaaaaaga caggcataat
      841 atattgcata tatttcagtg ccggattagc agcggtagaa gtcgtatcaa tctttttgaa
      901 attataccat gtatacaata atgttgtaac tgtccataat atagtaaaca aactcaaatg
      961 atggccaatc attggaatat tgaatgaaaa ttttgctata acatcatagc tagaaagatc
     1021 cgttgcccat aggaaagggg cttgtctaaa ttcaatcgct gcagggaaga agcgatacag
     1081 ggcaaaccat atcggcatct gtaacaacat ggggagacac ccgcccaatg gatttacacc
     1141 atattcacga tataggttca tggtctccat ttgtatctta gaagagtcat caccatattt
     1201 tgctttcaat ttgtcaagat gtggttttaa ggaagacatc ttagactggg agtaaatcat
     1261 tttataagtc aatggccata aagcaagctt gacaagtaaa gtcagcataa gtatcacaag
     1321 ccctttgctg gatatgaaag tcccgaggaa attaaacaat ggcctgacaa cccatttgtt
     1381 gatggaaccc attatactac ttccaaaggg aataatatct tccaaactgt tgttgtattc
     1441 tctgagtcta ttaaactcat taggtccggc atataccttc atcccaaagg attgaccgga
     1501 gctaaattct atcgggaatg tgacggttgc ttctgttttt tttaagtact tatcctctcc
     1561 ctcgccttgt gtaatggtca attcagcatt atcgaaaggc ttttcaggca cccaggatga
     1621 attaaaaaac tggttgacag ctgataacca ctcaagctgt tgcccgggtt taagtttgac
     1681 agtggtattc ttgctgtaag aagtataatc acttgattct ccggctattt tgaaatagag
     1741 cgtactatat gtacgttcat attgataatt tttctctagc ctcgtcaaat tattaaccca
     1801 cctgacagta ataggcttcg acttatccaa tccgggatcg ccaattatct tagcctgata
     1861 atcaatttga taagccgctg tatcaaattg ataagattgt tccaaaaaag cttgattggg
     1921 caactctgtt ttcaagataa ttttatcacc ggatttctca actgtagaag gtagagaaac
     1981 cgtttccaat ttacttccat tggctaatgg aaggatatat accatctcat tttcaggagc
     2041 ttcctgtaat aacaaaggtt cacgaacttc attacctttg gcatcttccg cactggtctt
     2101 atattgttta agatgaatct gtttgatctt ggcaccaaca ttggaaaccg ttagagcgat
     2161 taaatcattc tcaagggtta cctcagctgg aggttgcctt tttacaatac tggaagtgtc
     2221 gcttatcttg ataccggcag aatcattccg gggacttaat gcctccaaag ctttcaggga
     2281 atccgctttt tgctgcgtga ttttcgacaa cattacggaa tcctgcaatt tcttttctgc
     2341 ctgaattttc ttattattgt tttgatttaa cgtcatccaa acataaaaaa gaacggctag
     2401 taaaatgaaa ccaatgatac ttttacgatc catataagtt tctatatttt cagtaatttc
     2461 ttaattaaaa tttatttttc cacatcatac tttccatcgg cttgtcagac cgaacattgt
     2521 atttagctcc aattttctta ttataataat tgtcaatgtc tccttcgacc ttaaaataaa
     2581 tcaactggcc aaccggcatg ccggcataga ctcttacggg ttgagagaca ctaatctcca
     2641 gtgtccaatg gttgcaaaat ccaacatcgc cctttccggc agttgcatga atatctattc
     2701 ccaaacgacc aacactggat ttgccttcca gaaatggtac agtagcatgt gtttccgtat
     2761 attccaaagt aattccgagg taaagttttt cgggcattaa gacaaatcct tcttccggaa
     2821 tttcaaatag ttcaatagga ttatgttttc tggcatcaag tacttcatcg acataggtag
     2881 ccaggtattt acctaaatga acatcgtagg aatttgttcc aagacaggaa agttcgaatg
     2941 gttcaatcac gattgtccca tcgtcaattc cctgcaaaat ctgcttatcg gatagtatca
     3001 ttttatcaat aaaaagggag atcaaccaaa gccaatctcc caataattca ttatttgaat
     3061 aaatattatt cttcgataat ctcgaatgct actgtagatt gaacgtcctt gtggagattt
     3121 agcttagcac tgtaagttcc aagcgtttta atatcgtcct ccaaaatgat cttctttctt
     3181 tcaatttcaa caccaacctg atctttcagg gcattcgata tctgtatgtt ggttacggaa
     3241 ccaaatatct ttccgctcgt acctgctttt gctcctattt tcaaggtaac atcagtcaat
     3301 ttagcggcaa tttccttata ttcatccaca cgcttggcct ccttacgatc ttcttgtttt
     3361 accagctctg ccaacatctt tctgttacca gcatttgcta ccacagcaaa cccttgcggg
     3421 ataaggtaat tacggccgta tccatccttt accttaacaa tttcgtgttt gttaccgact
     3481 ttatctatgt cttttaataa tatgatatcc atttctctat tctactttaa agggttattt
     3541 taaaaggtct gcaacataag gcaacatggc aagatgtctt gcacgcttga ttgctgtagc
     3601 catttttctt tgatacttca atgaattacc ggaaattcgc cttggcagaa ttttaccttg
     3661 ctcattaata aactgcaata ggaaatcagg atctttgtaa tcaatgtgtt tgattccata
     3721 cttacggaat ctgcaatact tttttctgtt ctgtcccagg ttgggattac tgaggaactt
     3781 tatatcatct cttgaagcca tgttcaatta attattttaa ttttataaaa atttattctt
     3841 ctgttgattc gacttgattc tcctcaacaa caggtgcttg tgtttctttc ctttcttttg
     3901 cttcaggttg ttcttcctta gcttttactt tttcttttac tttgccaatc ttaccatttc
     3961 gcttgtcatc attgtacttg ataccaaact tatccaggct gatggtaaga aatctcatga
     4021 tacgctcatc tcttcgaaga gccaattcca acttggcaat gattgctccg gtttcagctt
     4081 gaaactcaat ggtgtaataa actcctgaag agcgtttctt aatgtcatag gcgagctgtc
     4141 tcaagcctaa ttcgtcaatt gcgacaatct tacaaccctc atttttaagc atatcggtgt
     4201 aagtgcctac tgtcgatttt aattcatcgc ccgacagtac tggatctacg atgaaagtta
     4261 cttcaaattg cttcattatt aaagaagtat ggtttaaaat aggttaaaaa ttaaaattag
     4321 ggtgcaaagg tatagttatt caataatata tacaaatttt gttaaagata atatgtaaat
     4381 actatccaat catattgcca aaacttgtct tattgaccaa tccgggccac cacttcatga
     4441 aaagaaaata tgtcctgaac acctgtcatc atatttttca atgaaatctt tccggccgtc
     4501 cgctctgcct ccccgatcag gcatacaaaa gcaaaatcca ggtcattggc ataggacatc
     4561 tgtttctgaa gttttgccac aacaggatat aactctgcgg aaacgccctg cactcttaat
     4621 tgattagtta caccgactgc gtatccgaaa gattcattat ccaggcaaac aataagaact
     4681 tgtttactcc tggaaatttg ctccggaaac aatttctttt cttccattac gtcataaatc
     4741 ctttcgattc caaatgaaat accaacaccc gatacatcct tcaaaccaaa gttagcagtc
     4801 aaattgtcat agcgtccccc gccacctata gaccccatat tggcattcaa tgcactgacc
     4861 tcaaatatac atccggtata atatccgagg ccccgggcca aagtggcatc aaactgcaaa
     4921 tggttctcta aaggcgcttc agagagatgg cccaggactt ctctcatctc aactattcct
     4981 agtgtgcccg tttcggaatt catatgttct tcaagctctt caagattagt cagagcaatc
     5041 agctttagga gatcccggat actttgtaca ggaaaaccct ttcctataag ttcacgttca
     5101 atgccttcct tgccaatttt gtccagcttg tctatgataa cagttacttc atgaaaatgc
     5161 tcgctgagac caacggtttg tgagatgcca aataatattt tcctgttgtt ccacctgata
     5221 ttgacaggca ggttcaatgc cgtaaataca tcatcataca atcggatcaa ctcagcttcg
     5281 taatacaatg aattagaacc tgcgacatcg gcatcacatt ggtagaattc ctgataacga
     5341 cccttttgtg gtttgtccgc tctccagaca ggttgtattg ctgaccgctt gaacggaaat
     5401 ttaatatgct gtctattcat tactatgtac cgcgcaaacg gaacggttag atcgtagcgc
     5461 aaaccacgtt ttgaaatatg gcgaatgata ccaaagctgt ctttgtctgt cagaaacgaa
     5521 tcttcaacgt cttttagaaa atctccgtta ttcagaactt taaagagcaa cttatcccct
     5581 tcctccccat aattaccggc caatgtttcc aaagcttcca tagccggggt ttctatggca
     5641 tcatatccat acaaggcaaa tttctcccga ataatattaa aaagataatt ccttttcttg
     5701 atctcaccgg gaagaaaatc ccgtgttcct ttaggcagtg ctggtttcaa tgcttgaatt
     5761 tttattttaa gtcccaaagg taggagtaaa aagaaaaata tttatccata cagcagcaaa
     5821 tttcgtttga catacgttac aatacatatc taatataacc gaaaggaatc attttctatg
     5881 gatataccca tctcgatgga tttcagagaa cgcgatctca tcagtgcctg catacgacgt
     5941 gaagaatggg ctatccgaaa agtctatgaa gaccattaca gcctgatgat gtcgatatgt
     6001 ctgagatttg ccgccaatga aagtgatgcc atggatcttc ttcatgacgg atacattaag
     6061 gtaattaaca attttgaaaa gtatgtcccc aacacttcct ttccttcctg gctacataga
     6121 ttgatagtca ataattgcat agatcatttc agaaaaatgt caagaagacg aacagaagac
     6181 atcgaacaga tataccatac agctataatc gatccggatg ctgtctccaa atgcacggag
     6241 aaagaaatcc tgaacgccat tcagaaatta agtccaacct atagaaccgt ctttgttttg
     6301 gcagctattg agggatattc gcacaaggaa attgccaccg agttgggtat aactgaaagt
     6361 acgagccgtt caaatttagt taaagccaga ttgcgacttc aggaattgtt aaaggaaaag
     6421 attggaatgt gatgttgcag gaaaaagaat ttgatagaat aattaagcat gcaatggatg
     6481 ggatgcaaat taagctccca actgatgcgt gggatcaatt ttctcagaaa acaggctgta
     6541 agaatctctc aagtcgcaca cgcttcgata aatgcgggaa tgtaggcctt tcacactatt
     6601 attctgcaat cggaccttca gtcggtgcac aattttctga aaatcaacaa gaatttcaaa
     6661 tacttgctga agaagaattt gatgcggcaa tcaaaaagaa acttaccgga aattcgatca
     6721 atcaaacacc taagacatca atcctgaata gtatactatt caggttcaac ctccataacc
     6781 acagactttt tatctataaa tgccttgaag catgtacatt ggccctattc ttttggatat
     6841 tcacctggca tatatattat gagcagaatt catcacaaca ggaaatgcct atggcagtta
     6901 tatctgaaga aagtaaaaca agtaatcata cattaaatat agcagcacat aatcaattag
     6961 aatcaacgag ttattccagg gaacctattc gaaatcatac tggaaataaa gtaacaaagg
     7021 atttaaatca aaagcaaatc cctgctattc aatatccgac cgggctatac ccgtcattgc
     7081 aggcttttac caaagagaca gatcatattg caaccaacca atattcttcc gatcacccaa
     7141 gtactgccca tgaagacttt catggcacca attggagtac tccggaaatt actccaatta
     7201 aaaaaatcga accgggcagg attgaaatat caaaaaagga gataacaatc agccctatcc
     7261 taccccaaca taaaaaagaa tcaaaaaata agtcattatg gctcgggcta aatgcaagtt
     7321 ggatatttga taaaatgtgg gccccgggat atattgcaaa cacaagagca atcattacca
     7381 ataggtttca gagtaaagga ttgggtgctg aagttgaatt cgggatagcg aaggcccaaa
     7441 ttgccaccgg cattaattat tcttataaaa aatttgacac cggaacaaaa gtccataatg
     7501 aaggccatta tatcaatata cctttaacag tcaaaaagga gttcggtcgg tcagaacaaa
     7561 tcattcctta ttgcaagatt ggacctgaaa ttacattggc tgcaacaaca tcttatactc
     7621 cgattatacc tgaaaagcca gacaataaag aagcctttgc cccactattt gtgcccgaca
     7681 agcaggtcag cgatggtatt ctgaaaggag atttcaagaa gaacagttac tttggcctac
     7741 acattgccct tggtattgaa gtacttataa agcaatcctc taaaattttt attgaagcgg
     7801 cacatcaggt caatccattc gaaaagggtg tcggagacaa taatcagcgt ctttcgtaca
     7861 ccactgtcaa tcttggattc aaaaaaaatc tgtatgaaaa ttgacaatta tccaaactaa
     7921 attcgatttt ttttcgtcta tatatgagga agtttaataa taattcaggt tgcctttcga
     7981 aaacgcatta aactttttaa acattttttt cattaagcat tgtacctttg tgcccgtttt
     8041 acgtcttaga attatatatg ttagaatctg ggatcaggta agaactattt ggttcgaaaa
     8101 acatttggaa tttttaacta aattataaaa catgaaaaaa ttatttggat tttggaactt
     8161 aatgttgctg gttggcgcat cattgttcgt agttagttgt aatccggacg ataaccctac
     8221 ttccagtaag ggaccaacgg ttactgaaac gcataccttt gatgcaacaa aagatcagga
     8281 cccacttctt ccaattataa tcaccgtcaa aggtgtcaaa ggagatgctg acctaaagtc
     8341 attgactgta aaagaaaatg gcacaaatct ggatgtcgat agatttcttg ttgatgacaa
     8401 tgatccaagt tcagctgcta tacttttatt aggtgcagac aaacaaggct ttactaaagt
     8461 atttgaaata gatccagttg caaaaaccgg agcatacaag tacgaattcg tacttacaga
     8521 tgagaaaaat aatacaaata cggtagcact tgaggtcaat ataaagactg ttgctaccat
     8581 aacatctgaa aaaatgtaca attacttagg cccattggca ggaggacttg atttgctgac
     8641 cggcaaggca gttgtaaaag gtgctgatgt taacaacgaa tgggctacag ctcatatcag
     8701 agacaatgga aatgatgttg tcggcaatac ttatccatgg aaaggtgaat ttataccctg
     8761 gaaaggaagt gtagtaagaa agacagataa ttccaactgg gatctaaccg ttagcccggg
     8821 tcagatttca tccttgtttg acaatggcct tacggatctg gctacagtaa aaatgaaggt
     8881 tggtgatgtc tatgtcgtta aaaacggaga tcattacttt atgattaaag tgaaggaagt
     8941 ttctgatgat ggaagt
//
LOCUS       DAOQQN010000086         8927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_2992_length_8927_cov_5.266344, whole genome shotgun sequence.
ACCESSION   DAOQQN010000086 DAOQQN010000000
VERSION     DAOQQN010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8927
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2992_length_8927_cov_5.266344"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PLG24_04705"
     CDS             complement(<1..335)
                     /locus_tag="PLG24_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HMZ24221.1"
                     /translation="MHLNIRILTIAILIIGLVAYTNRAIGQAEEKNIYEIGSLTITGA
                     KYSDENAIKTISGLRVGDEIQVPGPKITRAIRSLMNLKLFSDVVIIQEKTIGEVIFLE
                     IRVEERPRL"
     gene            complement(851..1477)
                     /locus_tag="PLG24_04710"
     CDS             complement(851..1477)
                     /locus_tag="PLG24_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HMZ24222.1"
                     /translation="MEPISYGYNTPLNTNLLPQNGVAIYYGNIMERCEADAYFDSLLS
                     NIAWQNDEAIMFGKRIVTKRKVAWYGDLPFEYTYSRISKYALSWTPELKRLKYITEKA
                     TGETYNSCLLNLYHDGSEGMAWHSDGEPDLKKYGAIASLSFGAERKFAFKHKISGQRI
                     DMILQHGSLLLMTGETQQHWVHRLPPTKKISTSRINLTFRTIIEKIRS"
     gene            1682..2992
                     /gene="murA"
                     /locus_tag="PLG24_04715"
     CDS             1682..2992
                     /gene="murA"
                     /locus_tag="PLG24_04715"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HMZ24223.1"
                     /translation="MKSDSFLVTGGVTLSGSIEPQGAKNESLQVVCAVLLTADTVIIR
                     NIPDILDIRLQMELLRGLGVKIEQLGPCDFSFNAADVNLSFLDSPEYLEKARRIRGSV
                     LLLGTLLGRFGKAYLPKPGGDKIGRRNLDAHFHGFEKLGAKFTYNEDAGHFEIDGSHL
                     EGAFIYMDEISVTGTGNVLMASVLTPGRTTIYNAACEPYLQQLCKMLVGMGAKISGIG
                     SNMLVVEGVEQLHGTEHRCLPDMIEIGSFIGMAAMTQSELTITNASVPDLGLILPTFE
                     KLGLKLEVRGDDIHIPAQDIYEIQTFIDGSIMTIYDAPWPGFTPDLMSIVLVVATQAR
                     GSVLIHQKMFESRLFFTDKLIEMGAKIILCDPHRATVIGLERKSRFRALNMSSPDIRA
                     GVSLLIAAMSANGTSRIDNIHQIDRGYQSIETRLNAIGAQIERI"
     gene            complement(3099..6683)
                     /locus_tag="PLG24_04720"
     CDS             complement(3099..6683)
                     /locus_tag="PLG24_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ24224.1"
                     /translation="MKKFFLLFTFFVLTIAGVKAQLPDGSIAPDWTLVDLDGNTHHLQ
                     DYLDQGKTVYIDMFATWCGPCWNYHQTHALENLYQQYGPGGTDQVMVFGIESDLTTPT
                     NCIYNVNCPSSKGDWTDGVSYPIIDCTSTNGPDFPNLYALSYYPTIYGICPNGQIWEI
                     GQRQLNGLVNFLNDCPQPPPLAVSYTTTDISCSLDPIGAIYLTVSGGKTPYTYSWSNG
                     ATSSNLTNIAAGTYSCTVTDNKGDQVFTGDIVVNGPSSLMSITSQSGQDATCEQPNGS
                     ASVEVTGGDPAYTYSWTNGATTSTITGLFEGSYGVNITDSKGCTISTSIQINNIPSPT
                     VMVSSDGQQLSCTNSTVTINSDGSSTGPQYQYSWTTNNGNIVSDPLAQEITVNAPGLY
                     VLTIFDTQLGCFNANFIQINGATGLPIANAGSDEVLPCSGGVITLNGGASSTGSNFTY
                     LWTTSNGNIVSDPNQITIDIDAPGTYTLKVTNTTNGCEASDQTSVTVDNSVAYTSTLH
                     EISCNGQADGAIEINQTNYTYNWSNGATTNSIHNLSPGVYQVTIVNSAGCTATASFTM
                     TQPEILSATYTGTDAQDANSNDGTASATPTGGKAPYSYLWSNGGTTQTITNLVPGLYT
                     VVVTDANGCTTSGSYAVNVQGCTLAATADVNDASCYGTTSGSITLSLSNVTGTPSILW
                     NTGATTSSLENIAAGSYSATIKDDGGCLTVVNVVVDQPSEIILSSVDQTNPACPQETT
                     GSITVNADGGSGNFNYVWNTGTTGNNITGLAAGNYTVTITDDSGCDVVKDIHLNSNDT
                     EKPNLRLKTSEIPLNENGTVDVTFDLINDGSSDNCSNYTILMNPEQFSCDDLGVRTIE
                     VTMTDAAGNKSVKTVDVTIVDKTAPQWNNCPAGEILANTCNGLVGLDPTYSDNCGQAQ
                     IQQIKGIDIKSEFPLGNTVQEYVVVDASGNSNTCSFIVHRDVELQLNSVYKDASCNQL
                     GSIEVITEGGSAPMQYSWDNGMEGNKNNVQPGTYKIIVTDNSGCAKELNLTIEGPYVY
                     DVESEEVTLPSGDNANGAIDITLNGDATGLTFEWSKDNEVFANTEDISGLTKGVYTLK
                     IKDGNGCVFGPFVYDLTVSGYDNRLDAQISIFPNPTKDVLSIRYEGKENNMNIQIFDG
                     LGRRVDMKRVTLTNGMVQISTQSLSNGTYRMIIDNGSKMTVKQFVVIK"
     gene            complement(6789..7964)
                     /gene="dinB"
                     /locus_tag="PLG24_04725"
     CDS             complement(6789..7964)
                     /gene="dinB"
                     /locus_tag="PLG24_04725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HMZ24225.1"
                     /translation="MYDRAILHMDLDSFFVSVERLRNSSLIGKPLIIGGDSVRGVVSS
                     CSYEARRFGVQSAMSMKMAKRLCPDAIVLRGDMEEYSKYSHLVTEVITDESPTFEKAS
                     IDEFYIDLTGMDRHFGCYRWSLQLTDKIVKETGLPISFGLSVNKLVSKIGAGEGKPQG
                     RVQVERGDEREFLAPLSTSKMPGIGTETYKRLSFMGVRTIRVLSEIPVKLLEREFGKT
                     GIILSRHANGIDDTPVQPYSEQKSISTETTLPQDTLDLKRLKSLLSSMTDKLAYELRS
                     SGWLTSCITVKIRYADFNTFTKQRHIAYTSGDRALQATVQDLFDKLYDRRQLIRLIGV
                     RFSGLVKGDYQISLFEDSESDLKLMQELDHIRDKWGMGAIMRASYMSPKSNIDKGNK"
     gene            complement(8026..>8927)
                     /locus_tag="PLG24_04730"
     CDS             complement(8026..>8927)
                     /locus_tag="PLG24_04730"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HMZ24226.1"
                     /translation="DYATASTHTSSAEVTLHELGHSFARLADEYYAGDSYAGEKVNMT
                     QETDPTKVRWKNWIGTDGVGIYQHCCGGNSALWYRPHQNCKMRTLNAPFCPVCTQTII
                     ERIHQLVSPIDAYEPIEATLTAVNDAITFRINPIPIIPNTLKVSWELNGQQVGDSSDV
                     LQVEVSDFSPGINDIIVNIEDTTALLRVDNHNTIHFKSVHWEVNKKTSGTYDVKSRLN
                     EYEILIYPNPAHDKIHVRIDQHIDIPIELQLTDMDGTIINRADVKPGEETTIEISHTT
                     PGLYFLRFYNKNYCIGLRKIIIE"
BASE COUNT         2280 a         1845 c         2078 g         2724 t
ORIGIN      
        1 aacagcctgg gcctttcttc tacccttatc tcaaggaaaa taacttcccc aatggttttt
       61 tcctggatga tgactacatc ggaaaataat ttcaggttca tcaaagatcg gattgccctg
      121 gttatcttgg gcccgggaac ctgaatttcg tcgcctaccc gaagtcctga aatggttttg
      181 attgcatttt catcactgta tttcgctcca gttatagtta aggagccgat ttcataaatg
      241 tttttttcct cagcttggcc aatggcacga ttggtataag ccacaagccc aattatcaga
      301 atggctatag ttaaaattct aatatttaaa tgcatcgcaa tcacaaagtt atagtgtata
      361 atcagctaaa ttttccattt ttgtcggaag ttcaacgagc tgaatatcag ttttttgagg
      421 cgcaaattta gagtattatt ggctactgac ctaacattct tcaatgaaac ctttttcaat
      481 aggaataata atctgaaaat cacaagtcaa acgttgtttt tccggaaaaa ttgtatggct
      541 tacaggcttg tattctatgt tctaggatgg ttcattcata tcgttgtgat atttctttaa
      601 ccatccaaac aattccgata aatggaaaag caattcatgt atcaattgga ttaaaatgag
      661 ttccttttat tatcaaacgt caaacctccc ggatgcgctg ccatccggtt gttgaatctg
      721 aatttttgta attattggac taaactaatt gttagtcagg actttttatt ggtaaaatat
      781 gatttattgc cttcagggaa gagccattat agtcaaaata tttcttgggc gggagattct
      841 ggcttatatt tcatgatctg attttctcta taattgtcct gaatgtcaga ttgatccttg
      901 aggtgctgat tttctttgtc ggtggtaagc ggtgcaccca atgttgctgc gtctctccgg
      961 tcatcaacaa cagactgcca tgttgaagaa tcatatctat tctttgccct gaaattttgt
     1021 gtttgaaagc aaattttctt tcagctccaa agctgaggga ggctatagct ccgtatttct
     1081 tcagatccgg ttcgccatca ctatgccagg ccatgccttc agaaccgtca tgataaagat
     1141 tgagcaggca tgagttgtaa gtttctccgg ttgccttctc agtgatatat tttagtcttt
     1201 ttaattcagg tgtccatgac aacgcatatt tactgatacg agaataggtg tattcaaagg
     1261 gaaggtcacc ataccacgct acttttcttt tagtgactat tcttttgcca aacatgatgg
     1321 cttcgtcatt ttgccaggct atgttggata gcaaggagtc aaaatacgcg tctgcctcac
     1381 atctctccat gatgtttccg tagtatatcg ccaccccatt ttgtggaagc aggttggtat
     1441 tcaatggtgt attatatccg taagaaattg gttccatatc cttttagcct gatgtaaata
     1501 taagtacaaa agaatgatta tcgcggtaag aatacatggt cattccattg aatgcgtatg
     1561 tagttttcga aaagttcatt ggttatgaga ttaaacagac tactgtattg tacgtttgaa
     1621 tatctttttt cagtaaaatt tctatattag gcttagcttt gaacctcaat gttagagaaa
     1681 gatgaaaagt gacagttttt tagttaccgg aggtgtaacc ctatcaggta gtatagagcc
     1741 tcagggcgcc aagaatgaat cgttacaggt ggtatgtgct gtgttgctga ctgcggatac
     1801 cgtaattatc aggaatattc cggatattct tgatatcagg cttcagatgg agttgcttcg
     1861 aggcttgggt gtcaagattg agcagctggg tccctgtgac ttttcattca atgctgctga
     1921 tgtcaatttg agtttcctgg actctccgga atatttggag aaggcaaggc gtatcagagg
     1981 atcggtcctt ttgttgggga ctttacttgg acgatttggc aaggcctacc taccgaaacc
     2041 ggggggcgac aaaattggca gaagaaatct tgacgctcat tttcatggat ttgaaaaact
     2101 gggggcgaaa tttacctata atgaagatgc cggccacttt gagatcgatg gtagtcacct
     2161 ggaaggagcc tttatctata tggatgagat ttctgtgaca ggaacaggaa atgtcctgat
     2221 ggcatctgtt ctgacaccgg gtagaacaac gatatacaat gccgcttgtg aaccttactt
     2281 gcagcaacta tgtaaaatgc tggtaggcat gggtgccaaa ataagcggaa ttggctccaa
     2341 tatgttggta gtggaaggag tggagcagct tcatggtaca gaacacaggt gtctgccgga
     2401 tatgatagag attggttctt ttatcggaat ggctgccatg acacaatcag aattgaccat
     2461 taccaatgca tctgtacctg atttgggatt gattcttcct acttttgaaa aattaggctt
     2521 gaagcttgaa gtaagaggcg atgatattca tataccggcg caggatattt atgagattca
     2581 aaccttcatc gatgggtcca tcatgacgat ttacgatgcg ccatggccgg gctttacacc
     2641 ggacctgatg agtattgtgc tggttgtggc aacacaagcc cgcggcagcg tattgattca
     2701 tcaaaaaatg tttgaaagcc gacttttctt tacggataaa ttgattgaga tgggagctaa
     2761 aatcatcctt tgtgatcctc atcgtgcgac cgtgataggg ctggaaagaa agagccggtt
     2821 tcgtgcgttg aatatgagtt cgccggatat ccgtgcaggt gtatcacttc ttattgctgc
     2881 catgtctgcc aatgggacca gcaggataga taacatacac cagatagatc ggggttatca
     2941 aagtatcgaa accagattaa atgccattgg tgctcaaata gagcgcatat aattccggag
     3001 cagagtccga gcatggcgaa taagccttaa tacattggtg gaaataaaaa actcccggca
     3061 taaaccggga gtttttacaa tacaaataaa ttaaaaaatt atttaataac tacaaactgt
     3121 ttcacggtca tttttgaacc attatcgata atcattcgat aggtgccatt ggagagtgac
     3181 tgagtggaaa tctgcaccat tccgttggtc agagtaaccc tcttcatatc gacccttctt
     3241 cccaggccgt caaaaatctg aatattcatg ttgttttcct tgccttcata cctgatggaa
     3301 agaacgtctt tggtcggatt agggaagatg cttatttggg catccagcct attgtcatag
     3361 cctgagaccg taagatcata cacaaaaggt ccaaagacac agccattacc atccttgatt
     3421 ttaagggtat aaacaccttt ggtcaatccg gagatatctt ctgtattggc aaaaacttca
     3481 ttgtccttgc tccattcaaa tgtaaggccg gtggcgtctc cattgagggt gatgtcgatg
     3541 gcaccattgg cgttgtcacc tgaaggcaaa gtcacttctt cactctcaac atcgtacaca
     3601 tatgggcctt cgatggtaag attcaattct ttggcacagc cggaattatc cgttacaatg
     3661 attttatatg ttcccggctg gacattgttc ttattgcctt ccatgccatt atcccaggaa
     3721 tattgcatag gggcagaacc gccctccgta atcacttcga tggagccaag ttggttgcag
     3781 gacgcatctt tatacactga gttcaactgc aactctacat ccctgtgtac tataaaggag
     3841 catgtatttg agtttccgga agcatcaacc acgacgtatt cctgaacggt attgcccaat
     3901 ggaaattctg atttgatatc gattcctttt atttgctgaa tttgtgcttg tccgcaatta
     3961 tctgaatagg tgggatccaa gcctaccaga ccattgcagg tgttggccag aatttctcct
     4021 gccgggcagt tgttccattg cggagctgtc ttgtctacga tagttacatc tacggtttta
     4081 accgatttgt tgcccgctgc atcagtcata gtaacttcaa tagtcctgac gcccagatca
     4141 tcgcaagaga attgttcggg attcattaat atagtataat tagaacaatt gtcggagcta
     4201 ccgtcattta tcaaatcaaa agtcacatca actgtaccat tttcattcaa tggaatttct
     4261 gatgttttaa gtctaaggtt aggtttctct gtatcgttgg aatttaagtg gatatccttg
     4321 accacatcac agccggaatc gtctgtgata gtgacagtat aattgcctgc tgccaatccg
     4381 gtaatgttgt tgccggttgt accggtattc catacataat tgaagttgcc tgaacctcca
     4441 tcggcattga cagtaatgct tcctgtcgtt tcctgagggc atgcagggtt tgtttggtca
     4501 actgaagaaa gaataatttc agatggctgg tcaaccacaa cattgactac ggtcaggcat
     4561 ccgccatcgt cttttatggt tgcgctatag ctacccgccg caatattttc aagagatgaa
     4621 gtagtggctc cggtgttcca gagtatggat ggcgtaccgg taacattgga caggctgagt
     4681 gttatacttc cggaggtggt tccatagcag gaagcatcgt tgacgtccgc ggtggcagcc
     4741 agggtgcagc cttgtacatt gaccgcataa cttccggatg ttgtacatcc attggcatcg
     4801 gtgaccacta ctgtataaag gcccggcaca agattggtga tggtttgtgt tgtgcctcca
     4861 ttagaccata gatagctata tggagctttt cctcctgttg gagtggcaga tgcagtgccg
     4921 tcattactgt tggcatcctg agcatctgtg cctgtgtatg tggcgctcag aatttccggt
     4981 tgagtcatgg taaatgatgc tgttgctgta catccggcac tgttgacgat ggttacctga
     5041 tagacaccgg gagacaggtt gtggatactg ttggttgtag ctccatttga ccagttatat
     5101 gtataattgg tttggttgat ttctattgct ccatcggcct gaccgttgca actgatctca
     5161 tgtagggttg atgtataggc tactgaattg tcaacagtta cgctggtttg gtcggaagct
     5221 tcacacccgt tggtggtatt ggttactttc agagtatagg tgccgggagc atctatatcg
     5281 atcgtaattt gatttggatc tgatacaata tttccgttgg atgttgtcca taggtaagta
     5341 aagttggatc ctgtagatga cgcaccgcca ttgagggtta tcactccacc gctgcaagga
     5401 aggacctcgt cactgcctgc gttggctata ggaaggcctg ttgctccatt tatttggata
     5461 aaattggcat taaagcatcc caattgggta tcaaatatcg tcagtacata taatcccggt
     5521 gcattcaccg taatttcttg tgctaatgga tcagatacta tgtttccatt gttggtcgtc
     5581 cagctgtatt gatattgagg tccggtggat gatccatctg agttgatggt aacggtagag
     5641 ttagtgcagc tgagctgttg tccatcactt gaaaccatta ctgttggaga gggtatattg
     5701 ttgatctgta tggacgtaga gatggtgcaa ccttttgagt cggttatatt cactccatag
     5761 gagccttcaa ataatccggt tatcgtggat gttgtggctc cattggtcca gctataagta
     5821 tacgccgggt cgcctcctgt cacttctact gaggcgcttc catttggttg ctcgcaggtg
     5881 gcgtcctgtc cgctttgtga ggtgatgctc ataaggcttg aaggaccgtt aactacgata
     5941 tcaccggtaa aaacctgatc tcccttgttg tcagtgactg tgcaggaata agtacctgca
     6001 gcaatatttg tcagattaga agaggtggct ccattcgacc agctgtaagt atagggcgtt
     6061 ttgcctccgc ttaccgtgag atagatggct ccaatcggat caagggagca actgatgtcg
     6121 gttgttgtat agcttactgc caatggagga ggttgcggac aatcgtttag aaagttaacc
     6181 aatccattga gttgcctttg tcctatttcc cagatttgac catttgggca gattccataa
     6241 atagtcgggt aataggataa tgcatatagg ttcggaaagt ctggtccgtt ggtcgaggtg
     6301 caatctatta ttggatagga aaccccatct gtccagtctc ccttagagct gggacagttg
     6361 acgttgtaga tacaattggt aggggtggtc aggtcagatt caataccgaa gaccattacc
     6421 tgatcagttc cgccagggcc atattgttgg tacaggtttt caagggcatg agtctgatgg
     6481 taattccagc atggtccaca ccaggtagca aacatgtcga tataaacggt cttaccctga
     6541 tccagatagt cctgcaagtg gtgtgtattg ccatctaggt cgacaagtgt ccagtccgga
     6601 gctattgatc catcaggtag ctgcgccttg actccggcga ttgttaatac aaaaaatgta
     6661 aacagtagaa agaacttttt cattaaaaaa taagttttga ttgaacagaa tgtaattgta
     6721 gtatttaagg gcaaatttag tattatctgc ataaaagcgc aaaaacactg gaataatatt
     6781 ttgagttatt atttattgcc cttatctata tttgatttgg ggctcatata gctggctctc
     6841 atgatggcac ccatgcccca tttgtccctg atatggtcta attcctgcat gagtttaagg
     6901 tctgattcac tgtcttcaaa gaggcttatc tggtaatctc ccttgaccag gccactaaaa
     6961 cggactccga taagcctaat cagctgcctc ctgtcataca atttgtcaaa aagatcctgg
     7021 acagtagctt ggagagctct gtcgccggaa gtatatgcta tatgtctctg tttggtaaat
     7081 gtgttgaaat cagcataccg tatcttcact gtgatacagg aggtcagcca gccggaggaa
     7141 cgcagttcgt atgccaactt atctgtcatg gaagacagta aggatttgag ccgtttgagg
     7201 tcgagggtgt cttgagggag agtggtctcc gtcgatattg atttttgctc ggaatacggt
     7261 tgtaccggag tatcatcgat accgttggca tgacgggata gaatgatgcc ggttttgcca
     7321 aattcccgct ccagcagttt cacaggtatt tcgctcaaga cacgtatcgt gcggacaccc
     7381 atgaagctga gccgcttgta tgtctcagtc cctatgcccg gcatcttgga ggtggagagt
     7441 ggtgccagaa attccctttc atctccgcgc tctacctgta cgcgaccctg tggtttaccc
     7501 tctccggctc cgatctttga taccagcttg ttgaccgaaa ggccaaagga gataggcagt
     7561 ccggtctctt ttacgatctt gtcggtcagc tgcagggacc atctatagca accaaagtgc
     7621 cggtccatac cggtcaggtc aatgtaaaac tcatcgattg atgccttttc gaaggttgga
     7681 gattcatcgg taatcacttc agtgaccaga tgactgtact tactgtactc ttccatatcg
     7741 cctcgaagca cgatggcatc cggacacaac cgtttggcca tcttcatcga catagccgat
     7801 tgcacgccga atctacgagc ttcatagcta cacgaggaca caacaccccg cacgctgtct
     7861 cctccaatga tcaggggctt gccgatgagg ctgctattcc taagtcgctc taccgaaacg
     7921 aagaatgaat ccaggtccat atgtaatatt gcccgatcgt acatttggca aaattacgaa
     7981 tattatgaca tttatgacga taatatcgtc tttgtttttg agattttatt caatgatgat
     8041 tttccggagg cctatgcagt agtttttatt ataaaaccgg aggaaatata atcctggtgt
     8101 tgtatgactg atctcaatag tcgtctcttc ccccggcttt acatctgccc ggttgataat
     8161 tgtaccatcc atatcagtaa gctgtaattc gattggaatg tcgatatgct gatcgatccg
     8221 cacatggatt ttgtcatgtg ccggattagg atatattagg atttcatatt cattgagccg
     8281 gcttttgaca tcataggttc cggatgtttt cttattgact tcccagtgga ccgacttaaa
     8341 atgaatggta ttgtgattat ccacccgcag caaggcagtt gtgtcctcga tatttactat
     8401 gatatcattg atccccggag agaaatcgga tacttcgacc tggaggacgt cggaactgtc
     8461 ccctacctgt tgaccattga gctcccatga aaccttgagg gtattgggaa tgattggtat
     8521 cgggttgatt ctgaatgtga tggcatcgtt gacagcagtc agggtagctt cgattggctc
     8581 ataggcatca atgggggaga caagctgatg tatcctttcg atgatggtct gagtgcaaac
     8641 cgggcaaaac ggggcattga gcgtcctcat cttgcagttc tgatgtggcc gataccatag
     8701 agccgaattt cctccgcaac aatgctgata tatgcccact ccatccgtac caatccagtt
     8761 tttccaccgg actttcgttg gatcagtttc ctgggtcata tttacttttt ctccggcata
     8821 ggagtcgccg gcataatatt catcggcaag acgggcaaag ctgtggccga gttcgtgtaa
     8881 tgtcacctcg gcggatgagg tatgtgtgga tgctgtggca tagtctc
//
LOCUS       DAOQQN010000087         8919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3000_length_8919_cov_5.388876, whole genome shotgun sequence.
ACCESSION   DAOQQN010000087 DAOQQN010000000
VERSION     DAOQQN010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3000_length_8919_cov_5.388876"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLG24_04735"
     CDS             <1..384
                     /locus_tag="PLG24_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HMZ24227.1"
                     /translation="DVEDLSKHKMEAEYFQIPEGSANAVNKAKTEGHRVCAVGTTSMR
                     SIESAVSAEGMLKAAEGWTNKFIFPPYPFSIADSMLTNFHLPKSSLIIMVCAFGGYDL
                     IMEAYHEAIKKDYNFYSYGDAMLII"
     gene            418..639
                     /locus_tag="PLG24_04740"
     CDS             418..639
                     /locus_tag="PLG24_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HMZ24228.1"
                     /translation="MYWTLELAHHLEDAPWPATRDELIDFAIRSGAPLEVIENLQEME
                     EEGEMFEGIEDVWPDYPRRDDFLFNEDEY"
     gene            660..1367
                     /gene="cmk"
                     /locus_tag="PLG24_04745"
     CDS             660..1367
                     /gene="cmk"
                     /locus_tag="PLG24_04745"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HMZ24229.1"
                     /translation="MNIGLFFLIHRVSNKINIAIDGFSGCGKSTLARAMAHELNYIYI
                     DSGAMYRAVTWLMIQKGVNPEDTESVHQVMDEMDLHFKRIDKVNHLYLNGRHLRDEIR
                     TMEVNNLVSQFSTISIVRSKLVQLQKKLAEKKGCVMDGRDIGTVVLKDAELKLFLVCE
                     MDIRVARRKKELEEKNIFTTFESVKENFIERDRIDSTRDDSPLQQASDAILLDNSHLG
                     PDQLLLKAMELAYARLG"
     gene            1369..1992
                     /locus_tag="PLG24_04750"
     CDS             1369..1992
                     /locus_tag="PLG24_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HMZ24230.1"
                     /translation="MELFIAVLASLFSVVNPIGALPVYTSMTSSYSIKERRRTALHTT
                     MYFTMICIIFFLGGSYILSFFGISIPALRLAGGLVILNSGFSLLNGKVIRAEAINEKM
                     KEEARQKEDISFSPLAMPLLSGPGSISLLISLYALHPSWHEKGIIIGVMFALGLIIYI
                     MLLSAPLIARMLGEAGMKAITRIMGFIVMAIACEYIVGGATSLISKQ"
     gene            2023..2673
                     /gene="upp"
                     /locus_tag="PLG24_04755"
     CDS             2023..2673
                     /gene="upp"
                     /locus_tag="PLG24_04755"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF001097.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HMZ24231.1"
                     /translation="MAHNFSLSNSYINQIVYELRHINIQKDRAKFRNNLEKFGILAGY
                     ELSRHFHYSAEEVQTSLGSTIIETLDDSIVIISILRAGIPLQYGLLKLLDNAETGFIA
                     AYRKEHPGGGFDIKMDYITCPDLSGKVVIFTDPMLATGSSLDTAMKMIENYGEPKELH
                     LISVIASEFAVEQIKRLHPSIRMWVGAIDEELTAKSYIVPGLGDAGDLAYGEKLQG"
     gene            complement(2937..3287)
                     /locus_tag="PLG24_04760"
     CDS             complement(2937..3287)
                     /locus_tag="PLG24_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HMZ24232.1"
                     /translation="MIEENTTEQILTLTPGAIRQLAIIRQEQSIPEDLGLRVGVKGGG
                     CSGFSYELGFDSYKMGDEVIIIDGLEVYMQKSHSIYLKGIEIDWEEGLNNRGFSFKNP
                     NASSTCGCGTSFSA"
     gene            complement(3384..3923)
                     /locus_tag="PLG24_04765"
     CDS             complement(3384..3923)
                     /locus_tag="PLG24_04765"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMZ24233.1"
                     /translation="MRRMAFLCIALLSYMASYGQGIPVSRDTSQSKAKAIKPVLAPAG
                     KEVIYQGAPVQNKFEISQEDGKIVVSPETMPSFPGGQPALEEFIAANMRYPEEALKAG
                     AEGIVVVKFTIEYDGTAHSPVILLDKVGHGANAETKRLVQLMPKWIPATLSQQPVTVE
                     FTMPVNYNLKKYHRVKGNK"
     gene            complement(3937..4455)
                     /locus_tag="PLG24_04770"
     CDS             complement(3937..4455)
                     /locus_tag="PLG24_04770"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMZ24234.1"
                     /translation="MANRLNILMGIGCFLCVSVTVIGQNDRIPAKQQDEVKLIEVKSK
                     QQKALNQATPEKNAKEIWWEENQVYTVVEDMPQYPGGTEAMYKFIGEHLVYPAEAKAA
                     GKEGLVVLSFIVEKDGSIHDIEILKDGVGFGAAEAASKVVSSMPVWSPGKQDGKPVRV
                     KITSPFKFILPK"
     gene            complement(4543..4986)
                     /locus_tag="PLG24_04775"
     CDS             complement(4543..4986)
                     /locus_tag="PLG24_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24235.1"
                     /translation="MICTGLQAQQPETAGGKAKPIKAREVKKAEKAAEVKAAEEVRDE
                     IYDEVDEMPVYKIGNKAFYSLMRQNFEALEREGVYVDKTMTLNFVVGKDGTVRDMVVL
                     DNKGEKVTESKTLRQNMPYWGPGRKQEKAVSTRMQVPIEFILEKN"
     gene            complement(5310..7172)
                     /locus_tag="PLG24_04780"
     CDS             complement(5310..7172)
                     /locus_tag="PLG24_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HMZ24236.1"
                     /translation="MKNLVRFLLPFFIIITWNMPGMADNGDPVRINPTNWWIGMEDNT
                     VQILIQGHSDGFNNQAVSINYPGVKLEKIHRLPNKHYFALDVSISPATKPGVVRIHIG
                     KDSYDWSLAMKNVKDNGKSYAQGVTAKDLIYLIMPDRFSNGDLSNDRIPGMKDQSLNR
                     DTVFNRHGGDLQGIINHLDYFNQLGVTALWLNPVLENDMPERTEHGYAITDHYRVDPR
                     LGGNEAYTKLVEEAHRKGIKILQDAIYNHIGLDHWSVTDPPSPDWLHHWEHYTQTTYK
                     DQVLMDPYASIADRKRMSDGWFVPSMPDLNQNNPYVANFLIQHAIWCVEQFGIDGWRI
                     DTYAYNDLGFMNRCNAALIREYPSISLFGETWVHGVINQSYFADNNYDIAYKSNLPGV
                     TDFQLLWGINDVMNKPFGWTDGVNKLYTTLANDFVYKDPYNNVIFLDNHDLSRAYSVY
                     GEDLKKYKMALTWLLTCRGIPQLYYGNEVLMKGTTYPNDGYVRLDFPGGWPGDSVNKF
                     TREGRSADENEVWDLISRLAHYRRNSVALTTGRLTQFVPEDGVYVYFRHAGDERIMCV
                     MNSMDTDKQIEVARFNELTDGYRSAKDVLGGSIISLQGKLNIPARTMVVWELLK"
     gene            complement(7177..8688)
                     /locus_tag="PLG24_04785"
     CDS             complement(7177..8688)
                     /locus_tag="PLG24_04785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HMZ24237.1"
                     /translation="MLTIDQVRDFKGKYPRQIWSLFFSEMWERFCFYGMRGMLVFFMT
                     RQLKFGDIQANLQYGATQAFVYAFTFVGGLFADKILGFRKSLYWGGFMMIIGSSLLAV
                     DPHQFFFLGLSFIIIGTGFFKPNISTMVGELYKDGDDRRDAGFSLFYAGINLGAFLGG
                     YFCVAIGKGNMFSGMIDEAHRWNVAFGLAAVGMIISLINFFYTQKSLGPIGLQPGHPH
                     ALVDTRAPGRWTEYAVYIGTLLLIPIIQIMVSKPEYTDYFMYTIGPVTLIYLFYEMSK
                     VTDKERKKLWAALIFIFFSILFWGIYEQSGGSLSIFAAKNLNDSLLGMKFDPNGVNNS
                     AGAFFIIALAPLFGLMWLWMAKRGFEPNTIIKFGLGFILLGLGYYTLFTTRFFATNGV
                     SSLDIFTIALLIITFGELCLSPIGLSIMTKLSPARLQGIMMGMWFLASAYGQYVAGLI
                     GASLAQAEADASATVTMEAYTDGYKMLGFYALIAGAILIAASPWIKKLMQGVK"
BASE COUNT         2551 a         2110 c         1795 g         2463 t
ORIGIN      
        1 gatgttgaag acttaagcaa gcacaaaatg gaagccgaat atttccagat tcccgaaggt
       61 tcggctaatg ctgtaaacaa ggctaagacg gaaggacatc gggtatgcgc tgtgggtact
      121 acatccatgc gatccattga atcagccgta tccgcagagg gtatgttgaa agcagcagag
      181 ggatggacca acaagtttat cttcccgcca tatcctttca gtattgcgga tagtatgctc
      241 accaatttcc acttgccaaa gtccagcctg atcatcatgg tatgtgcatt tggaggatat
      301 gatctgatta tggaagccta tcatgaagcc atcaagaaag attataattt ttattcctac
      361 ggtgacgcaa tgcttattat ttaatatatt aatcaacaat ttgtaaatcc ttaaactatg
      421 tattggacac tggaattagc acaccatctt gaagatgccc cttggcctgc cactcgtgat
      481 gagctgatcg actttgctat tcgttcaggt gcacctttgg aagtgattga gaatctgcag
      541 gaaatggaag aagaaggcga aatgtttgaa ggaatcgaag acgtttggcc cgattatccg
      601 cgaagggatg atttcctttt taatgaagac gaatattgat gcatgataaa ataagccgaa
      661 tgaatatcgg cttatttttt ttaatccacc gggtgagcaa taagataaat atagcaattg
      721 acggtttttc tggctgcggc aaaagcacac ttgcccgtgc catggctcat gaattaaact
      781 atatttatat cgattccggt gctatgtatc gagctgtaac atggttgatg atacagaaag
      841 gtgtaaaccc tgaagacacg gaatcagttc atcaagtcat ggatgaaatg gatctccact
      901 tcaaacgtat cgataaggtc aaccatctct acctgaacgg ccgacacctc agggatgaaa
      961 tacgaacaat ggaagtcaat aacctggtca gtcagttttc caccatctca atcgtaagaa
     1021 gtaagctcgt gcaacttcag aaaaaactgg cagaaaagaa aggctgtgtc atggatggca
     1081 gggatatcgg cacagttgtt ctcaaggatg ccgagttgaa gttattcctt gtttgcgaaa
     1141 tggatattcg cgtggcaagg agaaaaaaag agctggagga aaagaatatt tttactacat
     1201 ttgaatcggt aaaagaaaat tttattgaaa gggatagaat agattcgacc cgggatgata
     1261 gtcccttaca acaagcatct gatgctattt tgctggacaa ttcccatctc ggtccggatc
     1321 aacttctgct caaagctatg gaattggcat atgccagatt aggttgatat ggaattattc
     1381 attgcagtac tggcttcatt gttttcggtg gtcaatccca tcggtgcttt accggtatat
     1441 acttcaatga cgagcagtta ctcgatcaag gaaagacggc gtaccgcact gcatacgacc
     1501 atgtatttca ccatgatctg catcatattc ttcctgggcg gatcctatat cctgagtttc
     1561 tttggcatat ccattccggc cttgaggctt gcgggtggct tggttatact aaattccggt
     1621 ttcagtttat tgaatggcaa ggtgattcgt gctgaagcca tcaacgaaaa aatgaaagaa
     1681 gaagcccgcc aaaaggagga catcagcttc agcccgctcg ccatgccgtt actttcaggc
     1741 cctggctcca tatccttgtt gataagtctg tatgcgctcc atcccagctg gcatgaaaaa
     1801 ggtataatca tcggagtcat gtttgctctc ggcctgatta tctacatcat gttactcagt
     1861 gcacccctga tagccagaat gcttggtgag gcaggcatga aggcaatcac aagaattatg
     1921 ggctttatcg tcatggccat agcttgtgaa tacattgtcg gtggagccac aagcctgatt
     1981 tcaaaacaat gatctatctt tattcttata aattcctttg ctatggctca taatttctct
     2041 ctaagcaatt cgtatatcaa tcaaatagtg tatgaattaa ggcatatcaa catccagaaa
     2101 gaccgggcaa agtttaggaa taatctggaa aaattcggca tcctggccgg atatgagctt
     2161 tccagacact ttcattattc tgcagaagaa gtacaaacct ctctcggctc gaccatcatc
     2221 gagacactgg atgattcgat agtcatcatc tccatcctga gggcaggtat cccactgcaa
     2281 tacggacttc tcaaattgtt ggataatgct gagaccggat ttatagctgc ttacaggaaa
     2341 gaacacccgg gtggtggttt tgatatcaag atggattaca ttacctgtcc cgacctcagt
     2401 ggcaaagtgg tcatctttac tgacccgatg cttgctaccg gttcttccct cgataccgcc
     2461 atgaaaatga tcgaaaacta tggcgaacct aaggagttgc acctgatttc tgtcatcgct
     2521 tcggaattcg ctgtcgaaca aatcaaaagg ctacatccat ccatcaggat gtgggtagga
     2581 gccatcgacg aagaactcac cgccaagtcc tatatcgtcc cgggactcgg agatgccggt
     2641 gatcttgctt atggagaaaa actccagggc tgatacaaat ccctccacag tatgccgagc
     2701 ctcgcttgct gattaaactg aactattctg ttcctatgta aaggacggaa gaggtaaaac
     2761 aaaatggact actaaaatat gaaggtattt tgattttgtg attaaaagta tatatccagg
     2821 ggcgaattct cttttgttgc caataatttc cacgctagaa atattttact atcactaaaa
     2881 acaaaaaacc ggaccaaatg acccggctta attttaattt ttttaactgt ttcttcttat
     2941 gctgaaaacg aggtgccaca cccacaggtg ctgctggcat tggggttctt aaacgaaaaa
     3001 ccacgattat taaggccttc ctcccagtct atctcaatac ctttcagata gatactatgc
     3061 gatttctgca tatacacttc caatccatcg attataataa cctcatcgcc cattttatag
     3121 gaatcaaatc ccagctcata actaaaaccg gaacatccac cacctttgac acccacccgg
     3181 agccccaaat cttccgggat actttgttcc tgccttataa tggctagttg ccggatcgct
     3241 cccggagtaa gggtcaggat ttgttcggtt gtattctcct ctatcatcgt aaaattagat
     3301 tttgataatt atttaatgtt tttccacaat caaccatgat tcaacaacat atttctccaa
     3361 tcatagttca atacccggtc agtttacttg ttacctttga ccctatgata cttctttaga
     3421 ttgtaattga caggcatggt aaactccaca gtcacaggtt gttgggaaag ggttgccgga
     3481 atccattttg gcatcagttg caccagtcgt tttgtttcgg catttgcacc gtggcccacc
     3541 ttatccagca atatcacagg tgaatgtgcc gtaccatcat attcaatcgt aaactttact
     3601 actacgattc cctccgcacc ggctttaagc gcttcttccg gatacctcat attcgcggca
     3661 ataaactctt ccaaagcagg ctgtcctccc gggaaagaag gcattgtttc cggggaaact
     3721 actattttac catcctcctg agatatttca aatttattct gaacaggtgc accttgatag
     3781 attacttcct taccggctgg cgccaaaacc ggcttaatag ctttagcctt actttgggat
     3841 gtatctctgc taacaggaat accttgacca taacttgcca tatatgacag taaggcaatg
     3901 cacaaaaaag ccattctcct catcaaaaga ttcaaattat ttcggcaata taaacttaaa
     3961 aggactggta attttgactc gtacgggttt gccgtcttgt ttgcccggag accatacagg
     4021 catggaactt acgacttttg aggcagcctc agctgcacca aagccaaccc cgtccttcag
     4081 aatttcaata tcatgaatac ttccatcttt ttccacaata aaggaaagaa cgactaaccc
     4141 ttccttgccg gcagccttgg cttcagcagg atataccaga tgctcaccga taaatttgta
     4201 catggcttct gttccaccgg gatattgagg catgtcttcc acgacggtat atacctgatt
     4261 ctcctcccac cagatttcct ttgcattctt ttcaggagtc gcctggttca atgccttttg
     4321 ttgcttcgat ttaacttcaa taagtttgac ttcgtcctgc tgcttcgcgg gaatccggtc
     4381 attctgtcca attactgtca cagaaacaca caaaaagcaa ccaatcccca tcagtatatt
     4441 caatctattt gccatatgat taattttatt ttgatatata atatgcactc agattggaat
     4501 cccaaaaaat aaatgttatt cattcggatt tcctaaagta tcctaatttt tttccagaat
     4561 aaattcgata ggtacttgca tccttgtaga aacagccttt tcctgcttcc tacctggtcc
     4621 ccaatacggc atattctgtc taagcgtctt tgactcggtg actttttcac ccttattgtc
     4681 cagcacaacc atatccctga ctgtaccatc ttttccaaca acaaaattga gggtcattgt
     4741 tttgtccaca tacacccctt cgcgctccaa tgcttcaaaa ttctgcctca tcagactata
     4801 gaaggcctta ttgccaattt tgtataccgg catctcatct acctcatcat agatctcgtc
     4861 tcgtacctct tcggccgcct taacttcagc cgctttttca gcttttttaa cttcccttgc
     4921 tttgatgggt ttcgccttgc cacctgcagt ctccggttgt tgcgcctgaa gaccggtaca
     4981 gatcatcaaa aaaaatagta ttaagatatt gaacttcata ttcttacggt tgaaaggtaa
     5041 aggtacgaat aagcaaaaag ttataataat atttaaccaa aaattaagca ataaccacca
     5101 acgcttgatt atgctgtgct tgattagggc gtgcccctgt cgggtcgggt catccactgt
     5161 acttcgctac tcactcctga gagaggaagt gggtccaggg attgaaccga cttcgagtaa
     5221 cgatattttc agggaagaag gccgctcgtg ccgtttctac ccctcacgca tacggcacca
     5281 gtggagcgac atgacctaat tccaatcccc tacttcaaga gttcccacac taccatggtt
     5341 cgtgcaggga tattcaattt gccttgcagt gagataatcg atcctcccaa aacatctttg
     5401 gccgacctgt aaccatcagt caactcatta aagcgggcta cctctatctg tttgtctgta
     5461 tccatactat tcatcacaca catgattcgc tcatcaccgg cgtgcctaaa atatacatag
     5521 acgccgtctt cgggtacgaa ttgtgtcaat ctgcctgtag tcagggccac ggaattcctc
     5581 ctgtaatggg caagccgact gattaaatcc catacttcat tctcatcggc actcctaccc
     5641 tctcttgtaa atttattgac agaatctccc ggccatcccc ccggaaaatc aagcctgaca
     5701 taaccatcat tgggataggt tgttcctttc ataagcacct cgttaccata atacagctga
     5761 gggatgcccc ggcaagtcaa caaccaggtc agtgccatct tgtacttctt cagatcctct
     5821 ccatataccg aataagcgcg gctcaggtcg tgattgtcca gaaaaatgac attattgtat
     5881 ggatccttgt atacaaagtc attcgccagt gtagtataca atttattcac tccgtccgtc
     5941 cagccaaatg gcttgttcat cacatcattg atgccccaaa gaagttgaaa atcagttacg
     6001 cccggcaagt tacttttata ggcaatatca taattgttgt cggcaaaata gctttggttg
     6061 ataaccccat gtacccaggt ctccccaaag agggagatgg acgggtattc cctgatcaag
     6121 gcggcattac accggttcat aaacccgagg tcattgtaag catatgtatc gattcgccaa
     6181 ccgtcaatac cgaactgttc cacacaccag atagcatgtt gtatcaggaa gttggcaacg
     6241 tatggattat tttgattcag atctggcatg gaaggcacaa accatccatc gctcattctt
     6301 ttccggtcag cgatacttgc gtagggatcc attaggacct gatccttata ggtagtctga
     6361 gtataatgct cccaatgatg cagccagtcc ggtgacggcg gatcggtaac gctccagtga
     6421 tccagtccta tatgattgta tatcgcatcc tggaggattt tgataccttt cctgtgcgct
     6481 tcttcgacta gctttgtata agcttcatta ccacccagac gtgggtctac ccgatagtga
     6541 tcggtgatgg cataaccgtg ttctgtccgc tcaggcatat cattctccaa tacaggattt
     6601 agccataagg ccgtcacacc aagctgatta aaataatcca gatggttaat tatcccttga
     6661 agatctccac catgcctgtt gaacaccgta tcgcggttga gactctgatc cttcataccg
     6721 ggaatacggt cattggagag atcaccattg gaaaaccgat ccggcatgat aagatatatt
     6781 agatcctttg cagtcactcc ctgtgcatag gactttccat tgtcctttac atttttcata
     6841 gcaagtgacc agtcatagga atccttcccg atatggatcc tgactactcc gggctttgta
     6901 gctggcgata tggaaacatc cagtgcaaag tagtgtttat tcggaagtcg gtggattttc
     6961 tccagcttca ctcccggata attgatagaa accgcctgat tattaaagcc atcactatgt
     7021 ccctgaatca atatctgcac tgtgttatct tccataccga tccaccagtt ggtcgggtta
     7081 atacgcacag gatcgccatt atctgccata cccggcatat tccaggtaat gatgatgaaa
     7141 aacggcaaaa gaaatcttac taaattcttc atagtattat ttaactccct gcataagctt
     7201 tttaatccac ggcgatgcag caataaggat ggcgccggct atcaatgcat agaatcccag
     7261 cattttataa ccatcggtat aagcttccat agtaactgtc gctgaagcgt cggcctccgc
     7321 ctgggcaagg cttgccccga tcaatccggc tacatactga ccgtatgcag aagcgagaaa
     7381 ccacataccc atcatgattc cctgcaatct ggccggagac agcttggtca tgatggacaa
     7441 acctatggga gaaaggcaaa gctctccaaa tgtaataatc agcaaagcga tggtaaatat
     7501 atccagtgag cttactccat tcgtagcaaa aaaccgtgtt gtaaacaggg tatagtagcc
     7561 cagtccgagg aggataaagc ccaatccaaa cttgatgatc gtattcggtt caaatcccct
     7621 cttggccatc caaagccaca tgagaccgaa aagcggtgcc agggcaatga taaagaaagc
     7681 acccgccgaa ttgttgacac cattgggatc aaacttcata cccaacaagg aatcgttgag
     7741 gtttttggca gcaaaaatac tgagtgaacc accactctgt tcatagattc cccaaaagag
     7801 aatcgaaaag aaaatgaata tcaaagccgc ccacaatttc ttacgttcct tgtccgtgac
     7861 ctttgacatc tcatagaaca agtagatcaa cgttacaggc ccgatggtgt acataaaata
     7921 atcggtatat tccggttttg ataccatgat ctggataatg gggattaaca gaagagtacc
     7981 aatatagaca gcatattcgg tccatcggcc cggagccctg gtgtctacaa gtgcatgtgg
     8041 atgtccgggt tgaaggccga ttggtcccag gcttttttgc gtataaaaga aattgatcaa
     8101 actgatgatc attcccaccg cagccagtcc aaaagcaaca ttccatcgat gagcctcatc
     8161 gatcatccca ctgaacatgt tacctttgcc tatggcaaca cagaagtacc cacccaggaa
     8221 agcacccaga ttgataccgg cataaaacaa ggagaatccg gcatcccgcc tatcatcgcc
     8281 atctttatac aactcaccta ccatggtaga gatattaggc ttaaagaagc cggtgccgat
     8341 aataatgaag gaaagcccaa ggaagaaaaa ctgatgcgga tcgacagcca gcaaggagct
     8401 tccgataatc atcatgaatc cgccccagta tagggatttc ctgaatccga gaattttatc
     8461 ggcaaagaga ccgcctacaa aggtaaaggc ataaacaaag gcctgtgtgg caccatactg
     8521 aagattggcc tggatatctc cgaatttgag ttggcgggtc atgaaaaata ccaacatacc
     8581 gcgcatcccg taaaagcaga aacgctccca catctcagag aaaaacaagc tccagatttg
     8641 gcgtgggtat ttgcctttga agtcacgaac ctgatcaatt gtcaacatat cgcaagaaat
     8701 taaaaaggac gccttaagta aaacgtcctt gcaaattaat cattttattt taaaagaacc
     8761 ttatttaatc ccgcccatca ttttttgcaa tttgccactg atcaggaaca atataccggc
     8821 agcgacacca cacataataa tgaaaacaag gaagaattca tacaaattgg tgatctggaa
     8881 accaacaaat gaagtggttg gattgctctc actcggaat
//
LOCUS       DAOQQN010000088         8904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3009_length_8904_cov_4.907221, whole genome shotgun sequence.
ACCESSION   DAOQQN010000088 DAOQQN010000000
VERSION     DAOQQN010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3009_length_8904_cov_4.907221"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..926)
                     /gene="rsmH"
                     /locus_tag="PLG24_04790"
     CDS             complement(21..926)
                     /gene="rsmH"
                     /locus_tag="PLG24_04790"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HMZ24238.1"
                     /translation="MQEYHLPVLPRETIEILDIKPSGIYVDATVGGGGHSKLILERLN
                     DKGRLYVMDQDLDVVGNIPNDDRVIFIHSNFRYLRRMLRVEGVSLVDGILADIGVSSH
                     QFDTGERGFSFRFEGDLDMRMNRNREFTAADVLSNYDEAALVEMFSSLGEVRNAKTLA
                     KLIVDIRSKRPIKTIEEFVYGIQPAIMGNAAKYLAQVFQALRIEVNDELKALEDLLFD
                     AVEVLKPGGIMAVITFHSLEDRIVKNFFKTGNPEGRVVKDEYGHIFRPLIPITKKPIE
                     AGKEELMVNQRARSAKLRAARRSGD"
     gene            complement(926..1396)
                     /gene="mraZ"
                     /locus_tag="PLG24_04795"
     CDS             complement(926..1396)
                     /gene="mraZ"
                     /locus_tag="PLG24_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HMZ24239.1"
                     /translation="MQLYGEYECSVDEKGRLKLPVALLRHVGGNPPYKFYVNRGFEKC
                     LMLYPEKVWDEIVEKVNNLNSFVTEERNFIRYFFRGLRDVATDAAERILLPKPLIEFA
                     GIKKEVVLFGYMGRVEIWDKATYYDWADVEPDNFAQLAEKLLGNKREEENPTGL"
     gene            2723..3538
                     /locus_tag="PLG24_04800"
     CDS             2723..3538
                     /locus_tag="PLG24_04800"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HMZ24240.1"
                     /translation="MDQLKRTIEAAWDNRDLLNLEETRKEIRTVIELLDKGKLRVAQQ
                     EGDDWIVNEWVKKAVILYFPIQQMETIEVGPFEWHDKIALKKSFAEQGVRSVPHAVAR
                     YGSFLEAGVIMMPSYVNIGAYVGAGTMVDTWATVGSCAQIGSNVHLSGGVGIGGVLEP
                     VQASPVIIEDDAFIGSRCIVVEGVKVGREAVLGANVVLTQSTHIIDITGPEEVRYKGV
                     VPPRSVVIPGSYSKIFPSGTYQVPCALIIGKRKESTDKKTSLNSALREYNVAG"
     gene            3579..6239
                     /locus_tag="PLG24_04805"
     CDS             3579..6239
                     /locus_tag="PLG24_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HMZ24241.1"
                     /translation="MNKFILLFLAIGTISMSCNSTKPAPAQTAVTAPAGQLGEEPDSL
                     EEQEGYDYTMEDDNFHNPFGVEMTLPDTLPVYRATATKLFNLIDTKLWISFDWAKRHC
                     IGTAEITATPVARVRRVLDLDAVGFDIKTVTSPEGKNLKYNYDGNTLSIDLGKDYSVG
                     SQVKVKINYVAKPDENKIGGSSAISSDKGLYFINPDGTDKNKPRQIWTQGESQSNSRW
                     FPTIDRPNQRCTQEMWITVDDNFKTLSNGVLVDSRKNKDGSRTDHYKMSQPHAPYLFM
                     MAVGEYAIVHDKWDNIPLKYYVEPKFEKDAKAIFNHTPEMLSFFSELVGVKYPWPSFS
                     QVIVRDYVSGAMENTTAVIFGEFCQRTTRELLDGNNDGIVAHEMFHHWFGDYVTCESW
                     ANITMNEGFANYAEYLWEEHKNGRDAADFHRLNEMTGYFAASRNHMHDLIYYNYDNRE
                     DVFDAHSYNKGGLVLHMLRSYLGDEVFFAGLKLYLNKNAYTAVEAANLRLAMEEVSGE
                     DLNWFFNQWYFGSGQPELTVRDSIDASGKKLFLMVEQTQEGPEQQHIFQLPVKVDIYP
                     GGNAIPLTKNIFIDKRKQTFSFDVDGTPANIIFDADNVILGKIKFDKSKEAYMYQARH
                     NKTLYDRMMALDKIKSEITPEVDSIILGYLSDPSPVIRRKALEQVDLSGFPIFTSRVL
                     ELAEKDKEPSVRATALAILAENPKPEYEKTIVSNINDEVAYSVLASALSAYNKLDKDK
                     AEPFISKYEKENNSTITSVIAEIYKSNNDPRALTLMEGKLASSEGFEAFGIFGNYTDL
                     LLDRDKSTIIKGFETIEKMAKSSEFSQWKKFAVAKSLFDIAGATQSNPSFSEQESSDL
                     VKRSNAIVQSFIDKAGNERMKDAMSNLLIK"
     gene            6376..7248
                     /locus_tag="PLG24_04810"
     CDS             6376..7248
                     /locus_tag="PLG24_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HMZ24242.1"
                     /translation="MKHDRRTFIKEFGIAAAGLCFLPSCRSILPSNPDTVGIKKIGLQ
                     LYTLRDDMPKDPKGILAQVAQAGYHQIESYEGSSGIWWGMGPKGFRQYLSDLGMEAVS
                     AHCDIGKDFERKAEEAAEVGLKYLVCPWLGKQPNLDFYRKAAEDFNQKGEICRKYGIR
                     FAYHNHAYSFEKVGGTYPQDLMMKETDPALVDFEMDMYWVHAAHENINHWLEKYKGRF
                     KLCHIKDYSKNPGQDDGLNSVDVGKGVINWKDALTTAVNTGMKYFIVEQEAYPGTTPL
                     EAIRANAKYMMQFL"
     gene            7369..7851
                     /locus_tag="PLG24_04815"
     CDS             7369..7851
                     /locus_tag="PLG24_04815"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ24243.1"
                     /translation="MITWRNAKIEDLPTIGLLAGKIWEATYLDNIGRDQLEYMLNLFY
                     APETLLAKLEQGHNLYIINSDEKAVGYLHIESRPEDYFIHKFYIDTTIQNKGIGSHVI
                     KMIYEYIIQNNRPIRLQVNRKNYKAINFYFKNGFTIESVGDFDIGGGYYMNDFVMIKN
                     "
     gene            7875..>8904
                     /locus_tag="PLG24_04820"
     CDS             7875..>8904
                     /locus_tag="PLG24_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24244.1"
                     /translation="MKRYLSILLLTFTIMAVKAQGVMTPELLWSLGRVNGAFLHDNNK
                     SICYNVTTYDIKTGKRTNFISSMSIDGKNIKSISKDQPGDNPILLPDGNILYSANGEL
                     TSSGKVPDGLKDADNLLFNAKTGHAAWSQSIKIDKTAADMYPDYAATSARIYDDLMYR
                     HWAQWSDGTYNHVFVGKLQPDGSYKGIDIMPGEPHDAPTKPFGGSDDMAWHPDGNLLA
                     YVSVKKTGKEYAVSTNSDIYFYDLNTGKTTNFTLGMMGYDRSPAFSSDGKWLAWTSMP
                     RDGYEADKNSLWIANLTTGKKYDLFQNYDGNVNAFVWAPSNDKLYFSAPSNGLTSLFE
                     LKLNIDKSG"
BASE COUNT         2820 a         1941 c         1722 g         2421 t
ORIGIN      
        1 cttgtggcca cagggagatt ttaatctccg cttcttctcg ccgctcttaa ttttgcgctt
       61 cttgcacgct gattgaccat taattcttcc ttccccgcct caatcggttt tttggttatc
      121 gggattaaag gtctgaatat gtggccgtat tcatctttta ctaccctgcc ctccggatta
      181 cctgtcttga aaaaattctt gactatcctg tcttcaagac tatgaaaagt aatcacagcc
      241 atgattcccc cgggcttcaa cacttctaca gcatcgaata gcaaatcctc cagtgccttc
      301 agttcgtcat tcacctcaat cctgagggcc tgaaaaacct gagccagata ttttgccgca
      361 ttgcccatga ttgccggctg aataccatat acaaactcct caatggtctt aatcggccgc
      421 ttacttctta tatccacaat caatttagca agcgtctttg cattccttac ctcacccaga
      481 ctggaaaaca tctcgactaa agcagcttca tcataattgc ttaagacatc agctgcagta
      541 aattctcgat ttctgttcat ccgcatatcc agatcacctt caaaccggaa tgaaaaccct
      601 ctttctcctg tatcgaactg atgagacgaa actccaatat cagctaaaat tccatccacc
      661 agactcacac cctctacacg aagcattctc ctcagatacc gaaaattgct atggataaag
      721 attaccctgt catcattcgg aatattaccc actacatcca gatcctggtc catcacataa
      781 agccgacctt tatcattgag tctctcaaga atcagcttgc tgtgccctcc tccacccacg
      841 gttgcatcga cataaatacc ggatggctta atatccagta tctcgatagt ctcccggggc
      901 agaaccggca aatgatattc ttgcatcaca atccggttgg attttcctct tcacgtttat
      961 taccaaggag tttttcagcc aattgagcaa aattatccgg ttcgacatcc gcccaatcat
     1021 aataagtagc cttatcccag atctctacac gacccatgta cccaaacagc acaacttctt
     1081 ttttaattcc ggcaaattca atcaatggct tgggaaggag gatgcgttca gccgcatcag
     1141 tcgccacatc cctaaggcct ctgaaaaaat accggataaa gttgcgctct tctgtaacga
     1201 aagagttgag gttattcact ttttcaacga tttcgtccca aactttctcg ggatataaca
     1261 tcaaacactt ctcaaatccc cgattgacat aaaacttata cggcgggttt ccacccacat
     1321 ggcgcaacag agcgacaggc aacttcaacc tgcccttctc atcaacgcta cattcgtatt
     1381 ctccgtatag ctgcattaat taggttccaa gaactatttt ctgttcttct gtgtcaaaag
     1441 taatacaaac tcccacctac tgacacattt ttcccctata aaatttatta tttatatcaa
     1501 attattattt ttcatatatg aagataacca caataatgtt attttgtaaa tattttgatt
     1561 acatattata atatttaaat tatctatata ttaatacata tggtttacaa tataataagt
     1621 attatacttt attctcaatc taatctacag ccaagtggta aattatggga attatttcaa
     1681 taaaatctgc acatcatgcc atcaacctgg tttttttaac cggaaagtat accatcatgc
     1741 atacaccaaa catgagtaaa gagatgagct cgatagtata ttaatattaa atacattggt
     1801 cagtgctaac ccgattaagc ttccgtgaat aattgactgt ccggattttg ggcttttggg
     1861 tcacgtttgt gggaagtata taatatgcaa tagtgcctcc tcaattaaac tacctttata
     1921 tctttccagt cggttcacct gaatgaaatt catccgaatt ccacttaata aaatcctata
     1981 aggatcctca atcaaaaaaa acatcaaaaa aaatgtgatt cggtatgggt attgcctaat
     2041 attgaagtag gtagataatt aatactcaca atacctacaa ctactggtac cccgacacct
     2101 ccaatccaaa aaattacatc cataaggaaa aatttcaaaa cttaatacct caaacaaccc
     2161 ggcatcactg aatcccgggg tattaatctt ctacctagtt cgtttttttt tcaaaattca
     2221 acctaacccc atgaaatgtc actgatatgt acgtaccaaa tagctattcg aaggacgccc
     2281 ggtcgaaaat ttgaagtagg tagataatta atactcaaaa tacctacaac caccggtatc
     2341 ccgacacctc caatccaaaa aattacatca tcagggaaaa atttcaaaac ctcatacctc
     2401 aaacaacccg gcatcactga atcccggggt attaatcttc tacctagttc gtttttccct
     2461 aaccaaaaac tccaaccttg tttccccccc tttttcaaaa cctataatat ctcatattga
     2521 tcagacacca catcaattga aagaatatcc tctcctacaa tccgctccgc tttaatattt
     2581 accgtaaggc ctatgcggta taattccacc tgctactttg tcctaattat caatctcccc
     2641 atcgaccaaa tccgctattt cagggaactt attttatttt ctttgataaa atcctatctt
     2701 cgtgactcaa aactaaacaa cattggatca actaaaaaga actattgaag cggcctggga
     2761 taatcgtgac ttacttaacc tggaagagac cagaaaagaa atccgcactg tcatagagtt
     2821 acttgacaaa gggaaactta gggttgctca acaggaaggt gatgattgga tagtgaacga
     2881 atgggtcaaa aaagcagtaa tcctttattt cccaattcag caaatggaga ccattgaagt
     2941 tgggccgttc gaatggcacg ataaaatagc attgaagaaa tcatttgcgg agcaaggtgt
     3001 ccgttcggta ccccatgctg tggcccgcta cggaagcttc ttggaggccg gtgtcatcat
     3061 gatgccctcc tatgtcaata taggtgctta tgtcggcgct ggaaccatgg tcgatacatg
     3121 ggcaacagta ggttcatgtg cacaaatcgg gagcaatgtc catctcagtg gtggcgtggg
     3181 catcggtggg gttctggagc cggtacaagc aagtcctgtt atcatagagg acgatgcctt
     3241 cataggttcc aggtgcattg tcgtggaagg ggtaaaagtt ggcagggagg ctgtattggg
     3301 agctaatgtc gttcttaccc aaagcacaca tataatagat atcacaggac ctgaagaagt
     3361 tcgctataag ggtgtggtac ctccaagatc cgtcgtcatc cctggttcat attccaaaat
     3421 attcccatcg ggcacctatc aggtaccttg cgcccttatc ataggaaagc ggaaggaaag
     3481 taccgataaa aaaaccagtc tcaactcagc tcttagagaa tacaatgtag caggctgata
     3541 ttagttatta tattaactta acataaaacc aaaataaagt gaataaattc attctattat
     3601 ttcttgccat tgggactatt tccatgtcat gcaattctac caagcctgca cctgcgcaga
     3661 ctgcagttac ggcacctgcc ggacaacttg gggaagaacc tgattccctg gaagaacagg
     3721 aaggctacga ttataccatg gaagatgata atttccacaa tccatttgga gtggaaatga
     3781 ctttgcctga tacccttccc gtatatcgtg ccacagcaac aaaacttttc aatctgatag
     3841 acacaaaatt atggatcagt tttgattggg ctaaaaggca ctgtattggt acggctgaaa
     3901 tcactgcaac tccggtggca agggttagaa gagttttgga tctggatgca gtaggtttcg
     3961 atatcaagac tgtcacctcc cctgaaggta aaaacctgaa atacaactat gatggcaata
     4021 ccctgtcgat tgatctcggc aaagactatt cagtcggatc acaggttaag gttaaaatca
     4081 attatgtcgc aaaaccggac gaaaacaaaa ttggaggttc ttctgcaata agctctgaca
     4141 aaggacttta ctttatcaat ccggatggga ctgataaaaa caagccgcgt cagatatgga
     4201 cacaagggga atcccaaagt aacagcaggt ggtttccgac catcgacagg ccgaatcagc
     4261 gatgtaccca agaaatgtgg atcactgttg atgacaattt caagaccctt tccaatggcg
     4321 tgttagttga ttccaggaaa aataaggatg gaagccgtac tgatcattac aagatgtcac
     4381 aacctcacgc accatatctt ttcatgatgg cagtgggtga atatgccata gtccatgaca
     4441 aatgggataa tattcctctt aaatattatg tcgagcctaa attcgaaaag gatgctaagg
     4501 ctatcttcaa tcatacaccg gaaatgttgt cattcttctc agagctagtg ggcgtcaaat
     4561 atccatggcc gagtttttct caagttattg tccgggatta tgtctccggc gcaatggaaa
     4621 acaccacggc tgttattttt ggcgaatttt gccaaaggac aacacgagag cttctcgatg
     4681 gtaacaatga cggtatcgtt gctcacgaaa tgttccatca ttggttcggt gattatgtga
     4741 cgtgcgaatc atgggccaat attactatga atgaaggatt tgccaactat gcggaatacc
     4801 tttgggaaga gcataaaaac ggcagggatg ctgcggactt ccataggttg aatgaaatga
     4861 caggatactt cgctgcatca aggaatcata tgcatgacct tatttattat aactacgata
     4921 acagagagga tgtgtttgat gctcactcct acaacaaagg cggtctggtt ctgcacatgt
     4981 tacgttcgta cctcggcgat gaagtatttt ttgccggcct caaactatat ctcaataaaa
     5041 acgcttatac cgcagtcgaa gcagctaacc tgagactggc tatggaagaa gtatctggtg
     5101 aagacctcaa ttggttcttc aatcagtggt acttcggaag cggccaaccc gagcttacgg
     5161 taagggacag catagacgca tcaggaaaga aattgttcct tatggtagaa caaacccagg
     5221 aaggacctga gcaacagcat atcttccagc ttccggtcaa ggtggacatc tatccgggtg
     5281 gaaatgccat accattgacc aaaaatatat tcatcgacaa gagaaaacaa accttctcct
     5341 ttgatgtgga tggcacacct gccaatatca tctttgatgc tgataatgta attttgggca
     5401 aaatcaaatt tgacaaatca aaagaagctt atatgtatca ggcacgacac aacaaaacgc
     5461 tgtacgacag aatgatggca ttggacaaaa tcaagtccga aattacacct gaagtagaca
     5521 gtatcattct cggttatttg tctgacccat ctcctgtcat aagaagaaaa gcgttggaac
     5581 aagtagacct gtccggtttc cctatcttca cctccagggt actagaactt gcagaaaaag
     5641 acaaggagcc atccgttagg gctaccgctt tggcgatact agcagagaat cctaagcctg
     5701 aatacgaaaa aacgatcgtg tcaaatatca atgacgaagt ggcctattcg gtactcgcat
     5761 ccgccctctc agcttataac aaattagata aagacaaggc agaacctttt atcagcaaat
     5821 atgaaaagga gaataacagc accatcacgt ctgtcattgc agaaatatac aaatccaaca
     5881 atgatccaag ggctttgaca cttatggaag gtaaacttgc ctcctccgaa ggttttgaag
     5941 cattcggcat cttcggcaat tatacagatt tattgttgga cagagataaa tccactatca
     6001 tcaagggatt cgaaacaata gaaaaaatgg ccaaatcatc tgaattttca caatggaaaa
     6061 aatttgcggt tgctaaatcg ctttttgaca tagcaggcgc aacgcaatcc aacccttcct
     6121 tttcggaaca ggaatcttct gacctggtaa agcgttcaaa tgccattgtt cagagcttta
     6181 ttgataaagc cggtaatgaa aggatgaaag atgcgatgag caatctttta attaaataaa
     6241 aaacaacaaa tatatagtcg gatgaatctc caccgcaaga gcgatggaga ctttttttga
     6301 gtgtattata ttcccgttgg cttcattaac tcaagtttaa atactaaatt tacactaatt
     6361 taaatctttg gaaccgtgaa gcatgacaga agaacattta taaaggaatt tggtatagca
     6421 gcagccggct tatgcttttt accttcgtgc agaagtattc tgccctcaaa tcccgatact
     6481 gtaggaatta aaaaaatagg acttcaatta tacacactcc gcgatgatat gcccaaagac
     6541 ccaaaaggta ttttggcaca agttgcccag gcaggatatc atcaaataga aagctatgaa
     6601 ggttcttcag gaatatggtg gggcatggga ccaaagggtt tcaggcaata tttatccgat
     6661 ttgggcatgg aggcagtatc ggcacactgc gatatcggta aagactttga aagaaaggcc
     6721 gaagaagcag cagaagtcgg cttgaaatac ctggtatgcc catggcttgg caaacaaccc
     6781 aatcttgact tctatagaaa agcggcagaa gatttcaatc aaaaaggaga aatatgcagg
     6841 aaatacggta tccgatttgc ctaccacaat catgcctatt cctttgaaaa ggtaggtgga
     6901 acatatccgc aggacttaat gatgaaggaa acagatcctg cattagtcga ctttgaaatg
     6961 gatatgtatt gggtgcatgc agcacatgag aatatcaacc actggctgga aaaatataaa
     7021 ggcagattta agctttgcca catcaaggat tatagcaaaa acccaggaca agatgacggc
     7081 ttgaactctg tggatgtcgg caaaggtgtc atcaactgga aagacgccct tacaactgcc
     7141 gttaacacag gcatgaaata cttcattgtg gaacaggaag catacccggg aaccacacca
     7201 ctggaagcta tcagagccaa tgcaaaatac atgatgcaat tcctgtgatg atataaagac
     7261 ctgaatgggt catcattccg gtgtatagta accttgagca atttattatg ttttatccat
     7321 atcggataat cggattgtac ttaaaacctg aaagcttatc agcgcaatat gataacatgg
     7381 agaaatgcta aaatagagga tctacctaca ataggactac tagccggaaa gatctgggaa
     7441 gcaacctacc tggataacat cggacgtgac caattggaat atatgctgaa tctgttttat
     7501 gctcctgaaa cattattggc aaagcttgaa caaggtcata acctatatat catcaattcc
     7561 gatgaaaaag ctgtaggtta tctacatatt gaatctcgcc cggaagatta cttcattcat
     7621 aaattttata ttgacacaac aattcaaaac aagggcattg gctcccatgt tattaaaatg
     7681 atatatgaat acatcattca aaacaatcgg cctatcaggc tccaggtcaa tagaaaaaac
     7741 tacaaggcga taaattttta ttttaaaaat ggcttcacga tagaaagtgt aggggatttt
     7801 gatattggcg gaggttatta tatgaatgat tttgtgatga taaaaaatta attttcaaac
     7861 taaaaaatat gatgatgaaa aggtacttat ccattctttt actgactttc accattatgg
     7921 cagtcaaagc ccagggtgtg atgacccctg aattactatg gtctcttgga agggtaaacg
     7981 gagccttcct gcatgacaat aataagtcga tctgctataa tgtcactacc tatgacatca
     8041 aaaccggcaa aagaaccaac ttcatttctt ccatgagtat cgatggcaaa aacatcaaat
     8101 ctataagtaa ggaccaacca ggtgataacc caatcctcct cccggatgga aatattctat
     8161 attctgcaaa cggcgaattg acttcatccg gaaaggttcc cgatggatta aaagatgccg
     8221 ataatttgtt gttcaatgcc aaaaccggtc atgcagcatg gtcgcaatcc atcaaaatag
     8281 acaagacagc ggcagacatg tatcccgatt atgctgcaac cagtgcgcgt atatatgacg
     8341 accttatgta taggcactgg gcacaatggt ctgatggcac atacaatcat gtttttgtgg
     8401 gcaaactaca gccggatggt agttataagg gtattgacat tatgccgggt gaaccacatg
     8461 acgctccaac caagcctttc ggcggaagtg acgatatggc ctggcatccc gatggtaatc
     8521 ttttggccta tgtatccgta aaaaagactg gtaaggaata cgcagtgagt actaactcgg
     8581 atatttactt ctacgattta aataccggaa agacaaccaa ctttaccctt ggcatgatgg
     8641 gttatgacag gagtcctgct ttttcatcag atggaaaatg gctcgcctgg acttccatgc
     8701 ctcgtgatgg ctatgaagcc gataaaaata gcttatggat cgccaatcta acaaccggaa
     8761 aaaaatatga tttattccaa aattatgatg gtaatgtaaa cgcatttgtg tgggcgccct
     8821 ccaatgataa attatacttc tccgcccctt cgaatggcct tacatcctta ttcgagctga
     8881 aactgaatat tgacaaaagc ggat
//
LOCUS       DAOQQN010000089         8863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3026_length_8863_cov_4.395890, whole genome shotgun sequence.
ACCESSION   DAOQQN010000089 DAOQQN010000000
VERSION     DAOQQN010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3026_length_8863_cov_4.395890"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            758..1504
                     /locus_tag="PLG24_04825"
     CDS             758..1504
                     /locus_tag="PLG24_04825"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HMZ24245.1"
                     /translation="MQSESEAFYLQPFNIQYILSMNYLKYLGHSCFQFSIGGKTIIID
                     PFIRENALADGKVDVGNLEADFILLTHGHGDHVADAEELSLRNGATIISNFEVANWFQ
                     DRGCTTFGMNTGGKNDFGFGEVRMVKAVHSSSLPDGSYGGNPTGFLIHADGVTLYFAG
                     DTALTKDMELIPSFYHKPDLAILPVGGCFTMDYEDALIASKLLQCDRVIGCHFNTFPP
                     ISIDTVVAEELFAKNGKELILPEINQVINL"
     gene            1508..2296
                     /locus_tag="PLG24_04830"
     CDS             1508..2296
                     /locus_tag="PLG24_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ24246.1"
                     /translation="MGKIVAIANQKGGVGKTTIAINLGACLALMEYKVLIVDADPQAN
                     ASSGTGVTKEMTNYTIYDCFINEVPAVDAIVHSNTPNLDILPSDINLVGAELELVSRF
                     KREYIMKDFLEPIRDKYDYIFIDCLPSLGIITVNSLTAADSVIIPVQAEIFSLEGLGK
                     LKNTISLVRKQLNPHLTIEGIVLTMYDKRLRLANLVIEEVQEHAKDRVFDTIIHRNSR
                     ISEAPSSHQPVIMYDAGSKGTINFINLAHEFLKYQGAASEQATV"
     gene            2315..3196
                     /locus_tag="PLG24_04835"
     CDS             2315..3196
                     /locus_tag="PLG24_04835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HMZ24247.1"
                     /translation="MKKKDLGKGIRALLNSMDQEEAEPTIEAVRELSDGVAMIRISEI
                     ETNPDQPRSQFNDQQLNELAQSIKNFGIIQPLTLRKTGRNRFQIIAGERRFRAAQIAG
                     LQEVPAYIRLANDQELFEMALVENIQREDLNPIEIAISYKRLMEETNMTQENLSERIG
                     KDRSTIANYVRLLKLLPDIQATVKDKRLSMGHARALAGVDDIALQLAFYKQAMSNAWS
                     VRQLENNIRDHFHGKEVAKPTSRAKNPELEHLKDRLKKKFGVKVQVEQNEKGQGYLKF
                     IFNSTNELNGILDVIDG"
     gene            3247..3858
                     /locus_tag="PLG24_04840"
     CDS             3247..3858
                     /locus_tag="PLG24_04840"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HMZ24248.1"
                     /translation="MLHPLVKAIVPIVLIVISVHTLFCQNTDSLFLASPKDSSHLTKD
                     TVVTNWEGVDLKTPKKALIRSAILPGLGQAYNHQYWKIPIVYAGFGALIYAGEFNRKN
                     YRLLKEVYRNMIDGVPTDYDRYSRQSVRLARDNYRKNMELSYIALAGVYGLNILDAFV
                     SAHLKTFDINENISMKIKPAVQHEYIGLYAVPTGGLTFSFVLH"
     gene            complement(3850..4170)
                     /locus_tag="PLG24_04845"
     CDS             complement(3850..4170)
                     /locus_tag="PLG24_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HMZ24249.1"
                     /translation="MTSIKNFLNELPNKVIPAALEGVNTILHFDVTGDNGGLYTLRIA
                     DGKIDVSEGLIGDAKSVIRTSDKVFSDLVNKETNPMMALMTGKLKVSNPGEIMKYAKL
                     IGLM"
     gene            complement(4223..5587)
                     /locus_tag="PLG24_04850"
     CDS             complement(4223..5587)
                     /locus_tag="PLG24_04850"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMZ24250.1"
                     /translation="MKRTITDEEFRKKQPDPQIAGPINFRPDQFFQLDNGLKIILVEN
                     HKLPRVNVQLYIDRGVFADGDKAGLQELAGQLLSCGTVRDSKYEWDRKIDYYGGSIHT
                     TSIGGTASSLTRYFKQVFQLFADAILTPAFPLEEFNNLKRQLLSNLAAQKSEPESILG
                     NLIKRRIYPGRHPYSEVITEKTVRNITVDDCREWFLTNSSPDNSYLIFEGDITMEDIK
                     ILVGDNFSSWIPRSVTTLEWPEDMKRNGVNVSFVERPGAVQSLIGIGYSINYLPYNAD
                     TDAANLMNSVLGGYFSSRLNLNLRENKGYTYGIYSRLTNDESIGSFTTSVSVRNDVTA
                     DTILEIVKEMNTLRDVDISVEELQQVKNVISGNFSRSVENPMIIAKFAFARHKFNLPE
                     DYFKNQLIRINKVSVDDIRLMAQKYLLTDNMHIAVIGSREVLNSLTAFGQVTEFDFEG
                     NPIG"
     gene            complement(5596..6882)
                     /locus_tag="PLG24_04855"
     CDS             complement(5596..6882)
                     /locus_tag="PLG24_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMZ24251.1"
                     /translation="MSKNHLSIDFEEYDLDNGLHVILHKDNTSPLVSISVMYHVGSKN
                     ESPDRTGFAHFFEHLLFEGSENIARGQFFNIVQNAGGTLNANTSYDRTFYYEVFPSHQ
                     LETGLWLESERMLHATVDETGIETQRSVVKEERRQRYDNRPYGRIVEESMQRAYTVHP
                     YRWPVIGSMDHIDAASEEDYRNFYARYYVPQNAALCIAGDIDTDNAKGLILKYFGTIP
                     KGELPINRPEPNEPPMAHELRDIVYDNIQLPAVIHLYHIPAFAKGDYFAVKLLSDLLS
                     TGQSSRLNKVLVDEKRIAVQVGSFPLELEHPGVVLQFAIGNPDTDPEVLESAMDEEID
                     LILKEGLTDEDFEKLINQTETKLVNEQSTLLSIAEGLSTYYTYFGDTSMFNKQMDFYR
                     SVKKEDIRQAALKYFNKDNRVALFWLPEAHKIEKVE"
     gene            7041..8702
                     /locus_tag="PLG24_04860"
     CDS             7041..8702
                     /locus_tag="PLG24_04860"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HMZ24252.1"
                     /translation="MAVFKTLDKMIMKGFIIPFIATFFIALFILTMQFLWVWVDDLVG
                     KGVGALIILELLFYLLLSFFPIAFPIAVLLSSVMLMGGYAERYELSSMTSSGISLMRV
                     MKPLIFLSFFIACISFGFSNYLWPVANLKYRSRLLDIKNQKPTLSIQKGVFNRDFTGY
                     VIKVTDKDKDGRAVKGIMVYDNQRQIDKLNLITAKEGEMYTEPKDNLFIMKMFNGHQY
                     QEMNPQGSNEGRYPFVRMNFKEYTKVFDMSQFDFNRTDENLYKSHQSMLSIRQLKVAV
                     DSINSRLDRAVDDMNSGLMSSINSKYATTGYSAFNDRRESVDHQYFPKRDTQSMEKLK
                     RERLGIIPIKPQRKNTPAQIDEDFHSSPANLITSRDLFVNGNFKPGKPVFDQLNARFR
                     LASVNYAVSLAKSAQSTADLQSSIYAAEYDSKVRHIFEMHVKFAYSVICIVFLFIGAP
                     MGAIVRKGGFGYPLLVAIIFYMTYQVLFTIMKKSAESQVIPPALAPWIPVMVMALIGF
                     ALTRRAMSDGSMNLEVFSKFFTKIFDKASESYYQFVEFFLSWVKK"
BASE COUNT         2624 a         1657 c         1804 g         2778 t
ORIGIN      
        1 atgatccgga aaggacattc ataataataa attaatcata ttgttatatt ttttatacca
       61 caattaaatt ttagtactcc tttctcatca attggcagaa tctattgatt atcaaataat
      121 tccgtaaagt atttaatact ctggtttgcc ttttttaact tttcaatttc ggtttaaatc
      181 ggtttaaacc tatttattat aggtttttaa ggcatggcgg attagttttg tgagaaatgc
      241 aagcaattgt attaaataat attatctctt taattaaaag gggagattat gtattacact
      301 tcacttggag gttaatttcg aaaagattta agtgtatata ttgattgcct taattaagga
      361 cactgaaaaa aaaagaccga ccgggtgaat gctcataggt ctttttttta ttccctgatt
      421 atcatagcct gataaaaatt ttatctaaga accttaatca gattaatttt tagaaaagat
      481 aacttaaata tcaaaccttt gggcaacttc attcctggga gtgcaatttc ttggaaaaat
      541 agtggaatat gaccgccgga tactacgatc cataattccg gcattatata ataatggaga
      601 atatttttct gctcgtatat cgcaaatgcc aggttggaag tgtatttaac tgttattaag
      661 cataattcgt ttacttgtag tattaattca gacaatacaa acataatcca agccgggtta
      721 tataatcctt ggaggacttg agaaaaaatt tgtaactttg cagtccgaaa gtgaagcctt
      781 ttacttacaa ccctttaata ttcaatacat tctgtcgatg aattatctga agtatcttgg
      841 acattcctgc tttcaatttt cgattggagg taaaacaatc atcattgatc cgtttattcg
      901 cgaaaatgcc ttggcggatg gaaaagtgga tgtgggcaat cttgaagctg attttattct
      961 gcttacacat ggacatggag atcatgtagc tgatgccgag gaattgtcgc ttcgaaatgg
     1021 agctaccatt atttcaaatt ttgaagtagc taattggttt caggacaggg gatgtaccac
     1081 ttttggaatg aatactggtg gtaagaatga ttttggcttt ggcgaggtaa ggatggtaaa
     1141 agccgtacat agcagtagtt tgcctgatgg tagttatggt ggaaatccaa caggatttct
     1201 aatacatgct gatggcgtga ccttatattt tgccggtgat actgctttga caaaagatat
     1261 ggaactgatt ccgtcctttt accacaaacc tgacctggcc attttgccgg ttggtggatg
     1321 ctttacaatg gattatgagg atgccctgat tgcctctaaa ttattacaat gtgacagagt
     1381 catagggtgt catttcaaca ccttccctcc tatctccata gatacagttg tggcggagga
     1441 attatttgcc aaaaacggaa aagaactgat tctgcctgaa attaatcagg taatcaactt
     1501 ataatacatg ggaaaaattg tcgctatagc taatcaaaaa gggggtgtcg gaaaaactac
     1561 catcgccatc aatcttggag catgtctggc attgatggaa tataaagtac ttattgtgga
     1621 tgctgatccg caggcgaatg cctcttcagg aaccggcgtg accaaggaaa tgaccaatta
     1681 caccatctat gactgcttta ttaacgaagt accggccgtg gatgctattg ttcattccaa
     1741 tacgccgaat cttgacattc tgccttcaga tatcaattta gttggtgcgg agctggaatt
     1801 ggtaagtcgc ttcaaaaggg aatatattat gaaagacttt ttggagccaa tcagggataa
     1861 atacgattac atttttatcg actgtctgcc atctttgggt atcataacgg tcaattcact
     1921 tactgcagcg gatagcgtta ttattccggt gcaagctgaa atattttctt tggaaggttt
     1981 gggcaagttg aagaacacta tttcgttggt tcgcaagcag ttgaatcccc atctcaccat
     2041 tgaaggtata gtactgacca tgtatgacaa gcggcttcga ctggccaatc ttgtcatcga
     2101 agaggtgcag gaacatgcta aggatagggt gtttgacact ataatccaca ggaactcgag
     2161 gataagtgaa gctccatcca gtcatcagcc ggtgattatg tatgacgcag gaagtaaagg
     2221 aaccatcaat tttatcaatt tggcacatga gtttttaaaa tatcagggtg ctgcatctga
     2281 acaagcgact gtttaaccat ttaaaacatt agttttgaaa aagaaagatt taggaaaagg
     2341 aataagggca ttgttgaaca gtatggatca ggaggaagct gaaccaacaa ttgaagcggt
     2401 aagggaattg tcggatggtg tggctatgat cagaatttct gaaattgaaa ctaatccgga
     2461 tcaacccagg tcacaattca atgaccaaca gttgaatgaa ttggcgcaaa gtattaaaaa
     2521 ttttggaatc attcagcctt tgacccttag gaagaccgga aggaacagat ttcaaatcat
     2581 tgccggagag cgtcgttttc gagcagctca aatagccggt cttcaggaag ttcccgctta
     2641 tatacgtctt gccaatgacc aggagctgtt tgaaatggcc cttgtggaga atatccagcg
     2701 tgaagatctt aatccgattg agattgccat cagttataag agattgatgg aagaaaccaa
     2761 tatgacgcaa gaaaatctgt cagaaaggat tggaaaagac cgaagcacga tcgccaatta
     2821 tgttcgatta ttgaaactat taccggatat tcaggccact gtcaaggata agcggctatc
     2881 catggggcat gcaagggctc tggcaggtgt ggatgatatt gcgcttcaac ttgcttttta
     2941 caagcaagct atgtctaatg cgtggtcagt tagacagctt gaaaacaata tccgtgatca
     3001 tttccatgga aaagaggtgg cgaaaccgac atcaagagct aaaaaccctg aattggagca
     3061 tctgaaagac agacttaaga aaaagtttgg tgtcaaggta caagtggaac agaatgaaaa
     3121 aggtcaggga tatttgaaat ttattttcaa ctctacaaac gaattaaacg gtatcttgga
     3181 cgttatagat ggataaaagt caaatcaatc agaataaaag cttttaaaaa catcaaaatg
     3241 gccgatatgc ttcatccttt agtaaaggca atagtgccta tagtgttgat agtcatttcg
     3301 gtacatactt tattttgtca aaatactgat tcattatttt tggcttcgcc aaaagatagc
     3361 tcgcatctta caaaagacac agttgtaaca aattgggaag gtgttgatct caaaactccc
     3421 aaaaaagccc ttattcggtc cgccatttta cctggattgg gtcaggcgta caatcatcaa
     3481 tactggaaga tccctatcgt atatgccggt ttcggggctt tgatctatgc cggagaattt
     3541 aaccggaaga actatcgttt attaaaggaa gtttatagaa atatgattga tggggtgcca
     3601 actgattatg accggtattc gagacaaagt gttcggctgg cccgggacaa ctatcgaaag
     3661 aatatggagc tttcttatat tgcccttgct ggagtctatg gcttaaatat cctggatgct
     3721 ttcgtctctg ctcacctaaa gacatttgac atcaatgaaa atatcagcat gaaaatcaag
     3781 cctgctgtac agcatgaata tattggtctt tatgctgtgc caaccggggg attgacattt
     3841 tccttcgtcc tacattaatc ctataagctt ggcgtatttc atgatttcac cgggattgga
     3901 aacttttaac ttgccggtca ttaacgccat cattggatta gtttccttat tgacaagatc
     3961 actaaagacc ttatcgcttg ttctgataac cgatttggca tctccgatta aaccttcaga
     4021 aacgtcaatt ttcccatcag ctattcggag cgtgtagagt ccgccgttgt caccggtcac
     4081 atcaaaatga aggatggtat tgacgccttc taaagctgcg gggataactt tatttggcaa
     4141 ttcattcagg aaatttttga tagaggtcat tgcattgggt ttataatagt aatttattaa
     4201 atgagcagca aagctacaat ttttatccta tcggattgcc ttcaaaatca aattctgtaa
     4261 cctgtccgaa agctgtaagg gaattaagta cttcacgact accaataacc gcgatgtgca
     4321 tgttatcagt caataaatac ttttgtgcca tcagcctgat atcatcgacc gacaccttat
     4381 tgattctaat gagctgattc ttgaaataat cttcaggtaa attgaatttg tggcgtgcaa
     4441 aggcaaattt tgcaatgatc attgggtttt caactgacct tgaaaaatta ccgctgatta
     4501 cgttttttac ctgttgcaat tcttccacag aaatatccac atctctcagt gtattcattt
     4561 cctttacaat ttcaagaatg gtgtccgctg tcacatcatt tctgacgctt acggaagtgg
     4621 taaacgagcc aatggactca tcattggtaa gtcgtgaata tatgccatag gtatatcctt
     4681 tgttctccct aagattgagg ttgagcctac tcgaaaaata accacctaac acagaattca
     4741 ttaaattagc agcatctgtg tcagcattgt atggtaaata atttatggaa taaccaatac
     4801 caatgagtga ttggactgcg ccaggccttt ctacaaaact gacattcaca ccgtttctct
     4861 tcatatcctc aggccattcc aaagtagtaa ccgacctggg aatccaagat gaaaaattgt
     4921 cacccaccag aattttaata tcttccatag tgatatcacc ttcaaaaatc aagtaggaat
     4981 tatcgggcga cgaattagtt aaaaaccatt cacggcaatc atccacagta atatttctta
     5041 cggtcttttc tgtgattact tcactgtagg gatgcctgcc aggataaatc cttcttttga
     5101 ttagattgcc taaaatggac tccggttcag atttttgtgc ggccagattt gaaagaagct
     5161 ggcgtttcag attattgaat tcctctaaag ggaaagccgg ggttaaaatt gcgtctgcaa
     5221 agagttgaaa tacctgtttg aaatatcgag tcaaggaaga ggcggttcca ccaatcgaag
     5281 tagtatggat gcttccgcca taatagtcga ttttacgatc ccattcatat ttgctatctc
     5341 ttaccgtacc acaagataaa agttgcccgg ccagctcctg caatcctgct ttatccccat
     5401 cagcaaacac accacgatca atgtataatt gcacattgac tcttggtagc ttatgatttt
     5461 caaccagaat gatttttagc ccattatcca gttggaaaaa ctgatcaggc ctaaaattga
     5521 tgggtccggc tatttgagga tccggttgtt ttttgcggaa ttcttcgtca gtaattgttc
     5581 ttttcatcgt ttcctttatt caaccttttc aattttgtga gcttcaggca accagaataa
     5641 ggctacccta ttgtctttat taaaatattt tagtgcggct tgtcttatat cttctttttt
     5701 tactgatctg tagaaatcca tttgtttgtt aaacatagat gtgtctccaa agtaggtgta
     5761 atacgttgac aggccttcag ctatagaaag aagggttgat tgttcgttga ccagttttgt
     5821 ttctgtctga ttgatcagtt tctcaaaatc ttcatcggtt aatccttcct ttaaaatgag
     5881 gtctatttct tcatccattg ctgattcaag aacttcggga tcagtatctg gatttccaat
     5941 ggcaaattgt aacacgactc cgggatgctc cagttccaga ggaaaagatc ccacttgcac
     6001 ggcaattctt ttttcatcga caagcacctt gtttaacctg ctgctttggc cggtacttaa
     6061 aagatctgat aatagtttta ccgcgaaata atctcctttg gcaaaagctg gaatgtgata
     6121 aagatgaatt acagcaggta attggatatt gtcatatacg atgtcacgca attcatgtgc
     6181 catgggtggt tcattgggtt caggacgatt gatgggcaat tctcctttcg gaatggtacc
     6241 aaaatattta aggatgagtc cttttgcatt atctgtatca atatctccgg caatgcacaa
     6301 ggcagcattt tgaggtacat agtaccttgc ataaaaattc ctgtaatcct cctcgctggc
     6361 tgcatcgata tggtccattg agccaattac cggccatcga tacggatgta cagtataggc
     6421 tcgttgcatg ctttcttcca cgatcctccc atacggtctg ttgtcatacc tttgccttct
     6481 ttcttccttc actacagagc gttgtgtttc gattcctgtc tcatctacgg tagcatgcaa
     6541 cattcgttct gactcaagcc agagccctgt ttccaattgg tgggaaggaa agacttcgta
     6601 gtaaaaggtt cggtcatagg atgtattggc attcagagta ccacctgcat tttgaacaat
     6661 attaaaaaat tggcctcggg ctatattttc ggagccttca aagagcaaat gttcaaaaaa
     6721 gtgagcaaaa cctgttctgt ccggactttc atttttacta cccacatgat acatgacaga
     6781 aatggaaaca agcggcgagg tattgtcctt atgcaggatg acgtgaaggc cattgtccag
     6841 gtcatattcc tcaaaatcaa tcgataaatg attcttcgac attaatttaa ttatttcaac
     6901 agctatttac aataatataa taagccaaaa tgtttattga atcatgaata aaggttaaaa
     6961 atctttgtaa aaattgtttc ttttaaattt gcatcaacca attaatcaga ttttctcttt
     7021 atttgtgttt aaatacataa atggcagtat tcaagacatt ggacaaaatg attatgaagg
     7081 gatttatcat tcctttcatt gccactttct tcattgctct ttttattctt accatgcaat
     7141 tcctatgggt ctgggtggat gatctggtgg gcaaaggtgt aggagccctg attattttgg
     7201 aactattgtt ttatctgctc ttatctttct ttccgattgc atttccaata gcagttctat
     7261 taagttcggt tatgcttatg ggaggatatg ccgagcgata tgagctttca agtatgacat
     7321 cttccgggat ttcactgatg cgtgtcatga agccattgat ttttctttct ttcttcatag
     7381 cctgtatcag ttttggtttc agtaattacc tgtggcctgt tgccaatctg aagtatcgat
     7441 ccaggttgct ggatatcaaa aatcaaaaac cgactttgag cattcaaaaa ggcgtattca
     7501 accgggactt taccggttat gtgataaagg taacggataa agacaaagat ggcagagcag
     7561 tcaaaggaat tatggtgtat gacaatcagc gtcaaattga caagttgaat ctgattactg
     7621 ccaaggaggg agagatgtat acagagccaa aggacaatct tttcatcatg aagatgttca
     7681 atggacatca gtatcaggaa atgaatccac aagggagtaa tgaaggccgg tatccttttg
     7741 ttcggatgaa ttttaaagaa tataccaagg tttttgacat gagtcaattt gactttaaca
     7801 ggacggatga aaacctttat aaatctcatc agagtatgct gagtatcagg cagttaaaag
     7861 ttgccgttga cagcatcaat agtagacttg accgagcagt agatgacatg aacagtggct
     7921 tgatgagttc catcaattcc aaatatgcca caaccggata ttcagccttc aatgatagaa
     7981 gggaaagtgt cgatcatcaa tatttcccaa agcgggatac ccagtcaatg gaaaaattga
     8041 agcgcgagcg attgggtatt attcctataa aaccacaaag aaaaaataca cctgctcaga
     8101 tagatgagga ttttcatagc tccccggcaa atttaattac ttccagggat ttgtttgtaa
     8161 atggcaattt taagcccgga aaacctgtgt ttgatcaatt gaatgcccgt tttcgcctgg
     8221 ctagcgtcaa ctatgccgta tctttggcta aaagtgctca aagtactgcg gatcttcagt
     8281 cgagtatcta tgctgccgaa tatgactcta aagtaaggca tatcttcgag atgcatgtca
     8341 aatttgccta tagtgtgatt tgtattgttt ttctcttcat tggagcgcca atgggagcaa
     8401 tagttcgtaa aggcggattt ggttatccct tgcttgtcgc catcattttt tacatgactt
     8461 accaggtttt gttcacgatt atgaaaaaat cagctgaaag ccaggtcatc cctcccgcat
     8521 tggcaccttg gattccggta atggtcatgg ctttgattgg tttcgcttta acacgcagag
     8581 caatgagtga tggaagtatg aatcttgaag ttttttccaa gttcttcact aaaatttttg
     8641 ataaagcatc agaaagttat taccaatttg tcgaattttt tctcagttgg gttaaaaaat
     8701 aggctcaaat ctcgttatga tgttatcaaa tttctctgag cgatggactt tggtccctac
     8761 tgacgtattt tattgtatgc ccggtctaag acgcgatatt caggttattc tcctatcaat
     8821 gcacctgaga cattccacga actgaagctg cttccttccg gtt
//
LOCUS       DAOQQN010000090         8860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3028_length_8860_cov_6.089949, whole genome shotgun sequence.
ACCESSION   DAOQQN010000090 DAOQQN010000000
VERSION     DAOQQN010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8860
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3028_length_8860_cov_6.089949"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="PLG24_04865"
     CDS             complement(<1..811)
                     /locus_tag="PLG24_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HMZ24253.1"
                     /translation="MKSFDYIIIGGGSAGCLLANRLSDNAQTEVCLLEAGPSDDSHFI
                     HNTNPVNMLYLLNSPKYNWKYYAEGNHKTGKRRFFWPRGKTLGGSSAVNAMIYTRGHP
                     SDFDRWAALGNEGWDYRSVLPYFLKSERQHRGGDAYHNDTGEMDVIDPNFHFPASTAF
                     IEACKQAGIPHNRDINGEQYEGVDFFQINQTPDGQRAHSAVSFLDPIRHRKNLTIITG
                     AHVNKVLMNDGTAYAVEYFDLKNARRLCTLNARREIILSAGIIGSPQILMLS"
     gene            complement(1717..2928)
                     /locus_tag="PLG24_04870"
     CDS             complement(1717..2928)
                     /locus_tag="PLG24_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMZ24254.1"
                     /translation="MRILFLTDNFPPEVNAPASRTYEHCIEWVRRGVEVTVITCVPNF
                     PQGKVFDNYKNKWRQKETMDGIRVIRVWSYISANEGFGKRILDYVSFGVTSFLHGLFV
                     KTDLIVATSPQFFTAVSGRWLSLCKRKPWVMEVRDLWPESIKAVSAMKSDNMIFSYLE
                     WVEKRLYKSAKKIIVVTDAFRDKIASRGIDKNKISVVKNGVLMEKFKPVEKNRQLLSQ
                     LGLEGKFIFGYLGTHGMAHKLDFIVECAAKITDEDIHFLFIGNGAQKENVRNLAVQLR
                     VKNCTFLDSVPKNEIADYISITDVALVNLMKSETFQTVIPSKIFENASMQKPILLGVE
                     GESKSIIEQYDAGICFEPENEAAFLDAVYRLKSDAQLYGKLQEGCKRLALDFDRRKLA
                     LRMLEIFNQVT"
     gene            complement(2925..4298)
                     /locus_tag="PLG24_04875"
     CDS             complement(2925..4298)
                     /locus_tag="PLG24_04875"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HMZ24255.1"
                     /translation="MGGRKFRFINLEKDFGDKIDWNYEAFGKLWTYNLNYFEWLMQED
                     ISVEDGLKSIHEFINHYDEIKDGRESFPISLRLLNWIKFISENDIQDSRIDQVIHQDA
                     WRLYHHPEYHLLGNHLLENGFGLFFAGSYLNDDKLLGLAERILRSELEEQILPDGGHF
                     ELSPMYHQHMHFRVLDCIQLIHKNGHYSRQLLPLLTDKAEKMLGWLASMTFDDGTIPL
                     FNDSANNIAPTSIQLFTYAKELGLHVRKTELKESGYRRLKNDRLDCIIDVGNIGPDYI
                     PGHAHADSLSFELQVDGRPFIVDTGTSTYEVNEVRQQERSTSAHNTVVINDCNQSEVW
                     GGFRVARRVQVEVLCDEKNHIEAVVRGYSDKNISHTRRFTLESNSLIIEDEVKGSHAP
                     INNRCFFHFYPGVKPGYTSDISIITEGETSKKVMNFNYAPEFNKHIVSDCLEVKFDKK
                     LKVSISL"
     gene            complement(4530..6641)
                     /locus_tag="PLG24_04880"
     CDS             complement(4530..6641)
                     /locus_tag="PLG24_04880"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HMZ24256.1"
                     /translation="MKQIVQDLKSGQTILEEVPVPQVRPGCLLIKTHCSLVSLGTERM
                     LVEFGKAGWISKARQQPEKVMQVIQKIKTDGLRPTMDAVFRKLGEPLPLGYCNAGEVI
                     AIGKGVLGYTIGDRVISNGNHAEIVCVPENLTAKIPDEVSYEEASFTVIGAIALQGIR
                     LINPTFGETIVVTGLGLIGLLAAQLLKANGCKVIGLDFDGRKVDLAKSFGIEAIKVSS
                     EMDVPAAIMSHTNGIGADGVLITASTKSDEVISQAAQMCRKRGRIVLVGVIGLDIKRS
                     DFYEKELSFQVSCSYGPGRYDEHYEQKGLDYPIGFVRWTEQRNFEAVLSAIVGGGLKV
                     NELITERVILDEYNAIYGDMSKSGSIASIIQYPGDVDVSKTSVSVVSHDFSGGSAQIG
                     IIGAGNFTGAMIVPVLKELKADMKYIASAKGLSGTTLAKKYGIAHSTSRYQDILEDKD
                     VRAVLITTRHSAHAGQVIEALQHGKDVFVEKPLALNMEELDAVKDAYTSCGKSLTVGF
                     NRRFSPFSIAARECLGDGPIQVIATMNAGFIPANSWVQDMESGGGRIIGEACHYIDLI
                     TYFTGSRVEKVIMNAIGTHPSVSTDNASILLKYEDGSTGVINYFATGNKAYPKERIEI
                     FSQGKNILIDNFRKASFYGYKRSDMKKTQDKGHREQFSRWLQSVKNGGNPLIEFDDLY
                     NTSKAAILAVESLTEGRWMEL"
     gene            complement(6703..7857)
                     /locus_tag="PLG24_04885"
     CDS             complement(6703..7857)
                     /locus_tag="PLG24_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HMZ24257.1"
                     /translation="MNFELLVNTIQATHNQLQQSAIRSVNIHLTIRNWLIGFYIVEFE
                     QNGSDRATYGERLFAELAKTLNVKGLAETNLKLSRQFYLVYPQIAQIVSEQLQQIGFS
                     SIRHLSTDELQIHDNQTVKIRQTASDEFISTLPEGLQVPPNQLINSLSFSHFVLLLPI
                     EDPLKRTFYEIECMKGTWSVSELKRQINSLYFERSGMSTNPEKLSRLVNDKAHALVPT
                     DIIKSPFTFEFLGLKAKDVVYESDLEQALIDHLEEFLLELGYGFCFEAKQKRIIIGDR
                     YYHIDLVFYHRILKCHVLIDLKMKEVEHENIGQLKTYINYYKKNFMLKDDNPPVALLL
                     VTENNKELVEYAVADTDKDLFVSKYILELPSTEKLQEFISKELRALRDKL"
     gene            complement(7920..8162)
                     /locus_tag="PLG24_04890"
     CDS             complement(7920..8162)
                     /locus_tag="PLG24_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24258.1"
                     /translation="MKQIVQDFKSGQTILEEVPVPQVRPGCLLIKTHCILVSLGTERM
                     LVEFGKAGWISKARQQPEKMILYVNSTLSIDDFNLL"
     gene            complement(8159..>8860)
                     /locus_tag="PLG24_04895"
     CDS             complement(8159..>8860)
                     /locus_tag="PLG24_04895"
                     /inference="COORDINATES: protein motif:HMM:NF038364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AglZ/HisF2 family acetamidino modification
                     protein"
                     /protein_id="HMZ24259.1"
                     /translation="KYVGDPINAVKIFNEKEVDELMVLDIDATVEGRGPDFTMIKNLA
                     IECRMPFCYGGGVTTVDQAKNIIKLGAEKVALSSAAINDTSLLRKMGEAVGSQSVVVV
                     IDVKKKKGLLGKTTYEIYTKNGREKSGKKLENFLKELNDIGVGEIVINSIDRDGTMEG
                     YDNTLVKLVRDAVDIPITILGGAGSHEDIKSLIKDYKIIGAAAGSLFVFKGKYRAVLI
                     NYPNRIEKRNILNNQ"
BASE COUNT         2503 a         2038 c         1598 g         2721 t
ORIGIN      
        1 ccgagagcat caatatctgc ggcgaaccaa tgatgcctgc actcagaata atttcccgtc
       61 tggcatttaa agtgcacagg cgacgggcat ttttcaggtc gaagtattcc acggcgtatg
      121 ctgtaccatc attcataagc accttattga catgagctcc ggtgatgatg gtcaggtttt
      181 tgcggtggcg aataggatcc aggaaggaga cagccgaatg agcacgttgt ccatccggtg
      241 tttggttgat ctgaaagaaa tccacccctt cgtattgctc tccgttgata tcccgattgt
      301 ggggtatgcc tgcttgtttg caagcttcga taaaggccgt actagccgga aagtgaaagt
      361 taggatcgat gacatccatc tcacctgtgt cattgtgata ggcatcccct ccgcggtgtt
      421 gccgctcaga tttcagaaaa tacgggagca cagacctgta gtcccagcct tcattgccca
      481 gagccgccca ccggtcaaaa tcagatgggt gtccacgtgt atagatcatg gcgttgaccg
      541 cactggaacc gcccaatgtc ttaccgcgag gccagaaaaa gcgccgtttt cctgttttgt
      601 gattgccttc cgcataatat ttccaattat atttagggct attgagtagg taaagcatgt
      661 tgaccggatt ggtattgtgg atgaagtgtg agtcatccga tggccctgct tcaagcagac
      721 agacctctgt ctgggcattg tccgagagtc ggttggcgag gaggcatccg gcagatcctc
      781 cgccgatgat gatgtaatca aacgatttca ttgacgcata tcaatttcaa atgtgtatat
      841 agcatgatgg tgagtctcat acggaatggt aatcaggtag aatgctgtcc acacacagat
      901 tcaggtcttt ttgaggcggc ctttcgggac aatctatcat caaggtcgcg cctacagcgc
      961 ttatatccga aaacattgaa ttatacaaaa aaacattaaa tccttaggat atcaaaatca
     1021 atcagatcaa aagcctcgtt agaggcgacc ttatggtagc atgtcagcaa tcgcgaagca
     1081 tcaagccctg taggggcgac cttataaaaa acaaggcgtg cattaaatta taccataaag
     1141 ccatgaaggc aggttaagac atgcaagaca taataatcct tatgctcata atgattcaaa
     1201 aaaaataaaa ttcaatatta aatctttaac acattatgca tatcgactaa tcatgtcaat
     1261 ccaaaaggtc gcacctacgg agcttgtatc ctgctttatc ttaatatgct accataaggt
     1321 cgcgcctaca gcgcttaaat tcgaaatcat tgaattatac aaaaaaaatt aaatcttccg
     1381 gatatcaaaa tcaaccagat taaaaagcct cgttagaggc gacctgatta tcaagcaaag
     1441 acataaataa aattactatt gtgcagattt aagatttcaa tctacccttt aatggttata
     1501 actctgatca cagatattga ccaaaagtct tcataaatca ctcttccttt aaaaacaatg
     1561 tcatttccgt tactaatgga agatattatc catttctaat ggaagatatc tttcactgca
     1621 aatggaagat atggcaaatc agacgatggt aatgtaattg ccaaaaacct atatactgca
     1681 cgaaaaaacc tccacgctct gttgcaaatt actcattcaa gtaacctgat taaatatttc
     1741 cagcattctc agtgccagtt ttcttctgtc aaagtccagg gccaggcgct tacaaccttc
     1801 ctgaagtttt ccatataact gtgcatcgga ttttaatcta tacactgcat ccaaaaaagc
     1861 tgcctcattc tccggttcaa agcagatacc ggcatcgtac tgttcgatta tgctttttga
     1921 ctctccttcg acgcccagta aaataggctt ctgcatggat gcattctcaa aaatcttgga
     1981 aggaatcacc gtctgaaatg tttccgattt catcaggttg actagggcga catctgtaat
     2041 gctgatataa tccgcaattt catttttagg gacagagtca aggaaggtgc aattcttcac
     2101 ccttaactgt actgccagat ttctgacatt ttccttctgt gcaccattcc caataaatag
     2161 aaaatggatg tcttcatctg ttatcttagc cgcacactca acgataaagt cgagcttatg
     2221 cgccatacca tgtgtcccga gatagccaaa tataaacttt ccttccaggc ccaattgaga
     2281 gagcagttgc ctgttttttt caaccggttt aaacttctcc atcagaacgc cattcttgac
     2341 gactgaaatc ttgtttttat cgattccgcg gcttgctatt ttatccctga aagcatcagt
     2401 cacgacaata attttcttag cgcttttgta tagccttttt tccacccatt cgagatagga
     2461 aaaaatcata ttatcactct tcatggcgct gacagccttg atggattccg gccagaggtc
     2521 acgcacctcc atcacccagg gtttccgctt acataaagac aaccatcgtc cgcttactgc
     2581 cgtaaaaaac tggggcgagg tagctactat caggtcagtt tttacgaata aaccatgcaa
     2641 aaaagaggtc actccgaaac taacgtaatc cagaatccgt tttccaaacc cttcattggc
     2701 actgatataa ctccatacac gaatcacccg gataccatcc atcgtctctt tctgccgcca
     2761 tttgttttta taattgtcga aaaccttacc ctgtgggaaa ttgggtacgc atgtaatcac
     2821 cgtgacctcc acaccacgac gaacccattc gatgcaatgt tcgtacgtcc ggctggcagg
     2881 agcgttgact tcagggggaa aattgtctgt taaaaataat attctcaaag cgatatgctt
     2941 actttcagtt ttttatcaaa ttttacttcc agacagtctg atacaatatg tttattaaat
     3001 tccggagcat agttgaaatt cattactttt ttagaagttt caccctctgt gataattgaa
     3061 atgtcagaag tatatccagg cttcacaccg gggtaaaaat gaaaaaaaca tctattattt
     3121 atcggagcgt gactaccttt aacttcatcc tcaataatca aggaattgct ttccaatgta
     3181 aatcttctcg tatgcgaaat gttcttatcg gaatatcccc gaactactgc ttcaatatga
     3241 tttttttcat cacaaagaac ttcaacctga accctccttg ccaccctgaa tccaccccat
     3301 acttcagact gattacaatc attgatgacc acggtgttgt gcgctgatgt cgatctttcc
     3361 tgctgcctga cctcatttac ctcataggta gatgttcctg tatcgacgat aaacgggcgt
     3421 ccatccactt gtaattcaaa actcaaagaa tctgcatgcg catgacccgg aatataatcg
     3481 ggaccaatat tcccaacgtc tataatacaa tccaaccgat cgtttttcaa tctcctgtat
     3541 ccggattcct ttaactccgt ttttctcaca tgtaaaccta attccttggc ataggtaaat
     3601 aactgaatac ttgtcggtgc gatgttattc gcactgtcat tgaatagagg aatagtgcca
     3661 tcatcgaagg tcatcgatgc caaccatcca agcatttttt cagccttatc ggtaagtaac
     3721 ggtaataatt gccgactata atgcccgttt ttatgaatga gttgaataca atccagcact
     3781 ctgaaatgca tgtgctggtg atacatgggg ctcaactcaa aatgcccgcc atccggcaag
     3841 atctgttctt caagctctga cctcaggatc ctttctgcta aacccaacaa tttatcatca
     3901 ttcaggtaac ttccggcgaa aaacagtccg aatccattct ccagtaagtg atttcccagc
     3961 aaatgatatt ccgggtgatg ataaagccgc cacgcatcct ggtggattac ctgatcaatc
     4021 cggctatctt gaatgtcatt ttcagaaata aattttatcc agttgagcaa gcgtaaagat
     4081 ataggaaatg actccctgcc atcctttatt tcatcataat gattgataaa ttcatgaatc
     4141 gattttagtc cgtcttctac agaaatatcc tcctgcatca gccattcaaa ataattcaaa
     4201 ttataagtcc acaacttgcc gaaagcctca tagttccagt ctattttatc cccaaagtct
     4261 ttctcgagat ttataaatct gaattttctt cctcccatcc atgtttgtgg aaggatgatc
     4321 ctatttgaaa aaatcagctt ttccgcaacg tctttaaaaa caggtatttt ccgattaatc
     4381 cttagctttt tttcaagcaa gtaataaatc cggtatttaa tctgaatcca cttcagataa
     4441 cggactgtgt gaaaataaag aagcagtttg tgcaagagga gaattttaaa tcgtctttag
     4501 ttctgattga attatacaaa accactttat cacaattcca tccatcttcc ttctgtcagg
     4561 gactctaccg ccagaatagc agctttggaa gtattgtata aatcatcaaa ttcgatgagt
     4621 ggattaccac cattcttgac agactgtaac catcggctga actgctccct gtgcccctta
     4681 tcctgtgttt ttttcatatc gcttcttttg tatccataaa atgaagcttt tctgaagttg
     4741 tcaatcagga tattctttcc ttgtgaaaat atttcgattc tctctttcgg gtatgcttta
     4801 ttacctgtcg caaagtaatt aatcactccc gtagaaccat cttcatattt tagtaggatg
     4861 gatgcattgt ccgtacttac ggaaggatga gttccgatgg cattcatgat cactttctcc
     4921 acccttgaac ctgtaaaata ggttatcaga tctatgtagt gacaggcttc gcctatgatt
     4981 cttccccctc cggattccat atcctgaacc caggaattgg cgggaataaa tccggcattc
     5041 atggtagcga taacctgtat cggtccatcg cccagacact ccctggctgc aatggaaaat
     5101 ggtgagaacc gtctgttaaa gccgactgta agtgattttc cacacgaagt ataagcatcc
     5161 tttacagcat ccagctcttc catgtttaag gcaagtggtt tttccacaaa gacatctttt
     5221 ccatgttgca aagcttcgat gacctgtcct gcatgagcac tatggcgtgt agtaatcagc
     5281 actgctctta catccttatc ttccaggata tcctgatatc gggatgtaga gtgagctatg
     5341 ccatactttt tagccagagt ggtaccggat agcccttttg cactggcgat atacttcata
     5401 tccgccttta attctttcag tacaggcacg atcatcgcac ccgtaaagtt acctgcgccg
     5461 atgatgccta tctgtgcact tccaccggag aagtcatgtg agacgacaga tactgacgtt
     5521 ttagaaacat ccacatcacc gggatattgt attatagatg cgatagagcc acttttactc
     5581 atatcaccat agatggcatt atattcatcc agaattactc gttctgtaat aagctcattc
     5641 accttcaatc ctcctccgac tatagcactg agtactgctt caaagttgcg ctgttccgtc
     5701 caacgtacaa agccgatggg ataatccagg cctttctgtt cgtaatgctc atcataacgt
     5761 ccgggtccat aagagcagga tacctgaaag ctcaattcct tttcataaaa gtcagaccgc
     5821 ttaatatcca ggccaatcac tccgaccaat acaatccttc ctcttttacg acacatctgt
     5881 gcagcctgac tgatgacctc atcagatttg gtagaagcag taatcaatac accgtccgcc
     5941 cctattccat tggtatgact catgatggca gcaggtacat ccatttcgga agaaacttta
     6001 atagcttcta ttccgaatga ctttgccaga tcaacttttc gaccatcaaa atcaagtcct
     6061 ataaccttgc atccattggc tttgagcaat tgtgctgcaa gtaagccaat gaggcctaga
     6121 ccagttacga caatggtctc gccaaatgtt gggttgatca gtcgaatgcc ttgaagagct
     6181 atggctccga taactgtaaa ggaagcctct tcataggaga cttcatccgg aattttagca
     6241 gtgagattct ccggaacaca gactatttca gcgtggtttc cgtttgaaat aaccctatcg
     6301 cctatggtat atccaagcac tccttttccg atggcaatca cctctcctgc attgcaatat
     6361 cccagaggaa gtggttcgcc taatttccta aaaactgcat ccatggtagg tctcagccca
     6421 tcggtcttaa tcttctgaat cacctgcatt accttttcgg gttgctgacg cgccttactg
     6481 atccagccag ccttcccgaa ttctacgagc atacgctccg tgccaagtga aacaaggctg
     6541 caatgggtct ttatcaacag acatcccggt cttacctgtg gtacgggcac ttcttcgagg
     6601 atggtttggc ctgatttgag atcctggact atttgtttca ttaaatcgaa tttaaatttt
     6661 ggggaatcgt tacaaagttc ttcatctgca ttatcctgac acttataact tatcccttaa
     6721 cgctcttaac tctttactaa taaattcttg tagtttttcg gtactgggca attccagtat
     6781 atacttgctt acaaacagat ctttgtctgt atcagcaact gcatattcga ctaattcttt
     6841 attgttttca gttactaaaa gtaatgccac aggcggatta tcgtccttaa gcatgaagtt
     6901 ctttttataa tagttgatgt aggtttttaa ttgtccgatg ttttcatgtt ctacttcttt
     6961 catcttcaag tcaatcagca catgacattt tagtattcgg tgatagaaca ccaaatcaat
     7021 gtgatagtac cggtctccaa tgatgattcg tttttgtttt gcctcaaagc aaaagccgta
     7081 tccaagttct aataaaaatt cttcgaggtg gtcaataagg gcttgttcca agtcagattc
     7141 atataccaca tctttggctt ttagacctaa aaattcaaac gtaaacggcg atttaataat
     7201 atcagtaggc actaaggcat gcgctttatc attcaccaaa cgactaagtt tttcaggatt
     7261 ggtactcata ccactccgct caaaatacaa ggaatttatt tgtcgtttca gttcgctcac
     7321 actccaggtc cctttcatac attctatttc gtagaaagtg cgttttaacg ggtcttcgat
     7381 gggaaggagt aagacaaaat gtgaaaaaga taaagaattt atcaattggt taggcggaac
     7441 ttgcaggcct tccggcaagg tactaatgaa ttcgtcagag gctgtctgac gaatttttac
     7501 agtctgatta tcatgtattt gtaattcgtc agtcgacaaa tgacgaattg agctaaatcc
     7561 aatttgctgc aattgttcag acactatctg agcaatttga ggatagacta aataaaattg
     7621 acggctaagt ttgaggtttg tttctgccaa tccttttaca ttcaaagttt tggctaattc
     7681 cgcaaataac ctttcaccat atgttgctct atcagaacca ttttgctcaa attccacgat
     7741 ataaaaaccg atcaaccagt tgcgaatggt caaatgtata ttcactgacc ttatcgcact
     7801 ctgttgaagt tgattatgtg ttgcttgtat tgtatttact aacaattcaa agttcatagt
     7861 acaaggctat tcaagattat atacattcta ggaatcaatt aaatgcttat agtttagacc
     7921 tataatagat taaaatcatc aatactcaat gttgaattga catacaatat cattttttct
     7981 ggttgctgac gcgccttact gatccagcct gccttcccga actcaacgag catacgttct
     8041 gtaccgagcg agacaagaat acaatgggtc tttatcaaca gacatcccgg ccttacctgt
     8101 ggtacgggca cttcttcgag gatggtctgg cctgatttga agtcctggac tatttgtttc
     8161 attgattgtt taaaatattt cgtttttcaa ttcggttggg gtaatttata agtacagccc
     8221 gatatttacc cttaaatacg aataagctac ctgcagcggc accgattatc ttataatcct
     8281 taattaagga tttaatgtct tcatgagagc cggctccccc taaaatcgtt ataggaatat
     8341 ctactgcatc cctcactaat ttaactaatg tattatcata cccctccatg gtaccatctc
     8401 tgtcaatgga atttattacg atttcaccga caccgatatc attcagttct ttcaggaaat
     8461 tttctaattt ttttcctgac ttctcccgcc catttttagt atatatttca taagtggttt
     8521 tgccaaggag tcctttcttc ttcttaacat caatcactac aacaacactc tggcttccaa
     8581 cagcttcgcc catttttctc aataatgagg tatcattaat agcggcggaa cttaaagcca
     8641 ctttttcagc tccgagtttg ataatgtttt ttgcctggtc cacagttgta actccacctc
     8701 cataacaaaa aggcattctg cattcaatag caagattctt tatcatcgtg aaatccggac
     8761 ccctcccttc tacggtggca tcaatatcca ataccatcaa ttcatccacc tctttttcat
     8821 tgaatatttt aactgcgtta attggatcac ctacatattt
//
LOCUS       DAOQQN010000091         8847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3041_length_8847_cov_6.348726, whole genome shotgun sequence.
ACCESSION   DAOQQN010000091 DAOQQN010000000
VERSION     DAOQQN010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8847
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3041_length_8847_cov_6.348726"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="PLG24_04900"
     CDS             <1..1058
                     /locus_tag="PLG24_04900"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HMZ24260.1"
                     /translation="IIGMGRYRADSVFSVIDKAIMIVLLGWKLYWPGTTLPDLNFFMM
                     TYLLSVAITFFLALIFLFFVLKVQWARPDRAMIRQLLIASTPYALITFLMGAYGRLDA
                     VLLHSLLADGDFQAGIYAAGYRMLDAYMMFALLFANLLLPMLSSKQSDEEAFTDLMRF
                     VTGLVMAATIIIGVGGFVFRREISELLYHHSDEAWYNTLGLLFLTMIPVGLSTLYGTA
                     ILSIGRLKRMNILFSTGILLSIVLNLILIPEIKSEGAAVSALCVNSIIALGETIIFQR
                     ESGFKPGVSFLLKLMALLIISFAIFSGIYLIDTSFWLKIGLGAIAMFLLAIALGMIQL
                     NPRRIRAILSEKWTGTS"
     gene            complement(1101..1472)
                     /locus_tag="PLG24_04905"
     CDS             complement(1101..1472)
                     /locus_tag="PLG24_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HMZ24261.1"
                     /translation="MPHKKFICAVIGLSDNPERYAYKAAERLLLHGYPVIGYGLRPCT
                     VMGISVITNKHAVTGIDTVTLYVGPARQDAWLPLILDLAPRRVIFNPGTENHIIEQKL
                     IDSGIEVIEACTLVMLATGEF"
     gene            2298..3428
                     /locus_tag="PLG24_04910"
     CDS             2298..3428
                     /locus_tag="PLG24_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24262.1"
                     /translation="MKNWILLFLLLFSLSGGEVIAMTFQRQLAIDTIRCDSASTLFAD
                     TINSLIRKAEYREAFFMLDHWQEDCGENEINSRTSILLSMALGVHQDDAARQYFNNDF
                     HFVFRYRMEYSEMDDYQYNFWNHVTYFNLVPLRHPLDSALMGLAGRLYDKSSLLPNEK
                     LVCRLFMGDLRQFDKEVKKKGNSNTFISQYVKDDKRYISKQFLGVQFFSGIYASNDQG
                     RFLGNNASIGMGLYSPLKYRWQGNLQSRFRIMTHDNEFVFKALGNEYTVNSKLAFDFN
                     INFMYRYFENNHIAILPKAGIGLEYIGTGIKFYDEETEEPSSYNVSTMHTNIGVMALI
                     PVFLRNYAGIEVSYHYCPYGWSKGLISKFNNQAFTVELQFRF"
     gene            complement(3450..4349)
                     /locus_tag="PLG24_04915"
     CDS             complement(3450..4349)
                     /locus_tag="PLG24_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HMZ24263.1"
                     /translation="MDKKRVLIAGGSGFIGKNLYVELWNSKYDVFILSRNRKLCTRPD
                     FIYWAPENEIIEADGPLHFDCLINLCGEGIATKRWTAARKKVLMESRITPTRFIDSLV
                     RSGRLSITDYIGSSAIGIYGDRPGTSPCNETDVIEPKSFLADLCSQWEAAHLSLPESV
                     RKVILRIGLVLADEGGLLDQYRPLLSMRMVPYFGGGIPYMSWITAKDLCRMILFIMEH
                     PKTSGIYNAVADQPVISRDFAKAFIKSSGKKAFARGIPGFALKWMMGEQALLVLESQY
                     VSNKKIRMEGFRCLSTDISTAFG"
     gene            4424..4990
                     /locus_tag="PLG24_04920"
     CDS             4424..4990
                     /locus_tag="PLG24_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24264.1"
                     /translation="MLDLNVDNLLNKVHNYNAVFENTQKYRNVWRDSLKNQIISDVTE
                     ICKAVGLKIEIEATNQFSNLEAIICSLGNVKSGIHEIVEPDIEKHLIRFNGSLVYQQL
                     YNGKVLVVINYPIIENYMEPRPPKTIAIYRPEEIKTPFVLRHVETLVSEITAWEDYDD
                     DETVLPARIGFTHNLTPETTLKEDTQQQ"
     gene            5545..5802
                     /locus_tag="PLG24_04925"
     CDS             5545..5802
                     /locus_tag="PLG24_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24265.1"
                     /translation="MTWLASGLESACFYEKLLTITERKIGVNQAIAIKSTMPIHVPCF
                     RAKKPVVLSLVNEDIHVKMTPGFKQENIIFLLETFLIIFSN"
     gene            complement(5848..6183)
                     /locus_tag="PLG24_04930"
     CDS             complement(5848..6183)
                     /locus_tag="PLG24_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HMZ24266.1"
                     /translation="MGKFEVKTRKNGEFQFSLLAGNGQTILSSEGYSTKAACMNGIES
                     VRKNSANDALFERKVGANGKDFFVLKAANHQVIGQSQLYESKASMENGIESVKKNAPD
                     ATVDDQTGA"
     gene            6554..7861
                     /locus_tag="PLG24_04935"
     CDS             6554..7861
                     /locus_tag="PLG24_04935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ24267.1"
                     /translation="MKSYYLKNSIILMLFLITSSIYLIAQCPEGAITPTPGAALTIKS
                     GEMYCISSDISLLSVSIEEGGELILAPKVTVEANGIFNIDGYLTLYDYAGLKLTGAMN
                     QGSRYPADTKISIGNNAFISMTGSFTQFDPGANPAHPNAQAKIRLSNNAVMEICGTYT
                     QFSGNYPAVDYAGDMDHVAYFITKAPASGVGSSILSGDEETTWIAIGQVANLNPGDAS
                     YCGPNASPDKCDLWPSWLSAEYYNCFDAENNRDISLPIVLVSFDVVKNGLIASLTWAT
                     AVEYNHSGFEIQRSSDGINWSNLGFIAASQGFGVNTVTTRYAFDDLNPLPGNNFYRLK
                     QVDTDGSFSFSPVRTVGFMGKIDNLQVYPNPVNNVVNIAGVAPNSVIKIFDMAGNQVN
                     RKFTLSAMNSFDLSACKEGIYLIVVSDGSGNTETFKILKNKVP"
     gene            8053..8727
                     /gene="rpe"
                     /locus_tag="PLG24_04940"
     CDS             8053..8727
                     /gene="rpe"
                     /locus_tag="PLG24_04940"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HMZ24268.1"
                     /translation="MSGLIAPSILSADFTKLADEISMINRSEADWFHIDVMDGVFVPN
                     ITMGFLVVKAINQLAEKPMDVHLMTVHPELYIEQWRDAGADHITVHYEACTHLHRVIQ
                     QIKHTGAKAGVAINPHTPVKLLEDIIEDLDLVIVMSVNPGFGGQAFIPRTFDKVRALS
                     AMIRERNASTLIEIDGGVTLANAGALIESGAHALVAGNTVFNSPNPTETIHLLKKTCS
                     HTKHNA"
BASE COUNT         2507 a         1955 c         1801 g         2584 t
ORIGIN      
        1 ttatcattgg catgggccga tatagggcgg atagtgtgtt ttcggtcatt gacaaggcca
       61 tcatgatcgt cctgctggga tggaaactgt attggcccgg aacgacactt ccggatctca
      121 attttttcat gatgacgtat ttgttgtcag ttgcaataac cttcttcctg gcgttgattt
      181 tccttttctt tgtattgaaa gtccagtggg caaggccgga tcgtgctatg atcaggcaat
      241 tattgattgc atctacacca tacgcactga tcactttttt gatgggtgcc tatggaagac
      301 tggacgctgt gttgctgcat agtctgttgg cagatggtga ttttcaggcg ggaatttatg
      361 cggcaggtta cagaatgctc gatgcttaca tgatgttcgc attattgttt gccaatttac
      421 tattaccgat gttatcttcc aaacagagtg atgaagaagc ttttacagat ctaatgcgtt
      481 tcgtcaccgg gctggtgatg gcagcaacaa taatcatcgg tgtaggaggt tttgtttttc
      541 gacgtgaaat ctccgagttg ttgtatcacc acagcgatga agcctggtac aacactttgg
      601 ggttgttgtt cctgacgatg attccggtgg gactaagcac actgtatggc accgctattc
      661 tgtctattgg gcgcttgaaa agaatgaata tccttttttc caccggaatc ttgttgagta
      721 tagtcctcaa tcttatactg attccggaaa taaagtctga aggagctgcc gtcagtgccc
      781 tgtgcgtcaa ttcgatcatc gcattggggg aaaccataat attccaaaga gaatccggat
      841 ttaagccggg agtttccttc ctgttaaaat tgatggcatt attgataatc agttttgcaa
      901 tattctccgg aatatacctt atcgatactt ccttctggct aaaaatcggg ttgggagcaa
      961 ttgcaatgtt cctattggca atagcattgg gaatgattca actgaacccc cgtaggattc
     1021 gggccatcct atcagaaaaa tggactggaa cctcttgaac atttgatcac tgcattagtt
     1081 tcggaaaatg caccgatgat tcagaattcg ccggtggcaa gcatgaccag cgtgcaagct
     1141 tcaataactt caatgccaga gtcaataagt ttttgttcga tgatatgatt ctcggtgccc
     1201 ggattgaaga taacacgtcg tggtgcaagg tccaaaatca aagggagcca ggcatcctga
     1261 cgtgcaggac cgacatagag ggtcactgtg tcgataccgg tgacggcatg cttattggtg
     1321 attacggata tgcccattac agtacatggt ctcaggccgt aaccaattac agggtatcca
     1381 tgcaaaagca gtctttcagc agccttgtat gcatacctct cgggattgtc cgaaaggccg
     1441 atgactgcgc agatgaattt tttgtggggc atatgttact taaaacacag gatggcctaa
     1501 atggttttgg gacagaaagg gattgagaat aaagaggttt gccggccggg gaagacgggg
     1561 tttggggtgt cgtcacgaag taggtagaag aataataacc gctccggtaa acacagcaag
     1621 taatcccggg gattcaattt gaaaatttgc tttatcaggc aggctttatt tatttcatcc
     1681 ttctgaagtg cagacatacc gatggtttcc cgctattatt tatgtacata cttcattttt
     1741 tccggccccc aacccgacaa tcgcttcccg acatgatcaa aatgattccg gcaaaaacgg
     1801 cttcaccgaa gtaggtagaa gaataataac cgcgccggta atcacagcga gtaatcccgg
     1861 ggattcaatt tgaaaattag ctttatcggg caggctttat ttatttcatc cttctgaagt
     1921 gcagacatac cgatggtttc cggctattat ttatgtacat acttcatttt ttccggaccc
     1981 caacccgaca atcacttccc gacatgatca aaataattcc ggcaaaaacg gcttcaccga
     2041 agtaggtaga agaataataa ccgcgccggt aatcacagcg agtaatcccg gggattcaat
     2101 ttgaaaatta gctttatcgg gcaggcttta tttatttcat catcctgaag tgcagacata
     2161 ctgatggttt ccggctatta tttatgtaca tacttcattt ttcccgcaca ccaacccgac
     2221 aatcaataga taatcattcc ccctatcctc caattattac tgccgcaatt ctcacctttt
     2281 cgttaatttt ggacatgatg aaaaattgga ttttgctttt tttacttctg ttctctcttt
     2341 ccggtggaga ggttatcgca atgacgttcc aacgacaatt agccatagat acgatacgtt
     2401 gcgactcggc ttccaccctg tttgccgata ccatcaatag tttgattcgc aaggcggaat
     2461 atcgagaggc tttcttcatg cttgaccact ggcaggaaga ttgtggtgaa aacgaaatca
     2521 atagccgcac ttccatcctt ctttctatgg ctcttggagt ccatcaggat gatgctgcac
     2581 gacaatattt caacaatgat tttcactttg ttttcagata tcgcatggaa tacagtgaga
     2641 tggacgacta ccaatacaat ttctggaatc atgtgaccta tttcaactta gttccgctgc
     2701 ggcatccgtt ggattcggca ttgatgggct tggctggcag gctgtacgat aaatcatccc
     2761 tcctacccaa tgaaaagttg gtgtgccgat tgtttatggg tgatttgaga caatttgaca
     2821 aggaggttaa gaaaaaggga aactccaaca ccttcatcag ccaatatgtt aaggatgaca
     2881 agagatatat ttcaaaacag ttcttaggcg tacagttttt ctccggaatt tatgccagca
     2941 atgaccaagg tcggttttta ggcaacaacg cttccatcgg catgggcctt tactcgccat
     3001 tgaaatacag atggcaaggc aatttacaat ccagattcag aattatgacc catgacaatg
     3061 aatttgtctt caaggcattg ggcaatgaat atactgtcaa ttcaaagctg gcatttgact
     3121 tcaatatcaa cttcatgtac cgctatttcg aaaacaatca tatagccata cttcccaaag
     3181 caggtattgg tctcgaatac attggaacgg gaataaaatt ttatgatgag gaaacagaag
     3241 agccttccag ctacaatgta tccaccatgc ataccaatat aggggtgatg gccttaattc
     3301 ccgtcttcct gcgcaattat gccggaatag aggtgtcata ccattattgt ccttatggct
     3361 ggagtaaggg attaattagc aaattcaaca atcaggcctt cacagtcgaa ttgcagtttc
     3421 gattttaaga atttatttta acctaatggt caaccaaagg cggtactaat gtccgtactc
     3481 aggcaccgaa atccttccat tctaattttc ttgttggaaa catattggct ttccaacaca
     3541 agcaatgctt gttctcccat catccacttc agtgcaaatc cgggaatacc tcttgcaaat
     3601 gcctttttac cactactttt aataaatgcc tttgcaaaat cacgacttat aacaggctga
     3661 tctgccacgg cattataaat tccgctggtc ttaggatgct ccattatgaa gagaatcatc
     3721 cgacataggt cctttgccgt tatccaggac atataaggaa ttcctccacc gaaataaggg
     3781 accatacgca tagagagaag gggtcggtat tggtccagca ggccgccttc atcagctagt
     3841 acaagcccaa ttcgtaaaat gactttacga accgattcag ggagcgatag atgcgcggcc
     3901 tcccattgac tacacaaatc tgccaagaaa cttttcggtt ctatgacatc tgtctcatta
     3961 caaggagatg tccccggtcg gtctccatag atcccgattg cagaagatcc aatataatcc
     4021 gtaatactga gtcttcctga acgaaccaac gaatcaatga agcgtgtcgg tgttatcctg
     4081 ctctccatca gaaccttctt ccgggctgca gtccaccttt ttgtggcaat gccctctccg
     4141 caaagattaa ttagacaatc gaagtgaagt ggaccatccg cttcgatgat ctcattttct
     4201 ggtgcccaat agatgaagtc cggtcgggta cacagttttc tattacgcga caaaatgaaa
     4261 acatcatact tgctgttcca caattcaaca tagaggtttt tcccgataaa cccgctgccc
     4321 cctgcaatca agactctttt tttatccatt ttggcaaaat tcacaaaatt ggataaatat
     4381 atgtacatta gtccaataat ttaaaaacta taaaacgaaa gtgatgctcg acctcaatgt
     4441 ggacaatctg ctcaacaagg tacacaacta caacgcagta ttcgaaaata cccaaaaata
     4501 ccgcaacgtt tggcgggaca gcctcaaaaa tcaaatcatc tcggatgtca ctgagatatg
     4561 caaggcggtt ggcttgaaaa tcgagatcga agcgaccaat caattcagca acctggaggc
     4621 tatcatttgc tccctgggta atgtcaagtc cggcatccac gaaatcgtag aacccgatat
     4681 tgaaaagcat ctgattcgct tcaatggaag tctggtatac cagcaacttt acaatggaaa
     4741 agttcttgta gtaatcaatt atcccatcat cgaaaactat atggagcccc gaccacccaa
     4801 gaccattgcc atttaccgtc ctgaggaaat caagactccc tttgtgctga ggcatgtgga
     4861 aactcttgtt tcggagataa ccgcctggga ggattatgat gacgatgaaa ccgtattgcc
     4921 ggcacgtatt ggttttacgc ataatcttac tccggaaaca actttgaagg aagatacgca
     4981 acagcagtga tgataagatg ttccttgcat tttaatcaca tttcctgaaa tcttcgaatg
     5041 ccgggttaaa tccactcgcg catcgcagtt gaggtctgca ggcaaagtaa tcaatacttc
     5101 aaaataaaat ttaattgcag tcgggtgtgg ataggtagcc tgggacattg gtcattaaag
     5161 atacagaaac agcgtggcag tacacgggaa ctgccggtta gatattgtcg attgttggac
     5221 aaccaccatt ctcgcaggaa tttcattcgt gatcggttga ataagtctta tcccaaaagg
     5281 caatcgtttt caaccttatt acgtaaaaca atgatgactc aacgggattt ctaagtatgg
     5341 tatttttatg cctgtttgat gaattacctt tgaatcgggt cttttcagaa aatggctcaa
     5401 tggaaatagg atcccaaacc ttacaaaatg tgatattaaa aaacaaaaca gctttaatct
     5461 ttcacctggc aatgattctt cttactatta ccatcatcag cgtgtcaaaa agtcattcct
     5521 agggcgctct acttaaaatc tcagatgact tggttagcct caggattaga atctgcttgc
     5581 ttttatgaaa aattgttgac catcacagag aggaagattg gtgtaaacca agcaatagcc
     5641 atcaaaagca cgatgcctat tcacgtcccg tgttttcgtg ctaaaaaacc ggttgtttta
     5701 tctttggtca atgaagatat tcacgtaaaa atgacgcctg gctttaagca agagaatatc
     5761 atttttctct tagaaacctt tctgattatc ttctccaatt aggtatatag atgtaaaaaa
     5821 agcccgggag aaaacccgga ctttttatca tgcacctgtc tgatcatcga ccgtggcatc
     5881 aggagcattt ttcttaacag actcaatgcc attttccatg gaagctttgc tttcatacaa
     5941 ttggctttgt cctatcactt gatgattggc tgctttcaat acaaaaaaat cctttccatt
     6001 ggctccgacc ttccgctcaa aaagggcatc attcgcagaa ttctttctta cagattcaat
     6061 gccgttcatg caagccgctt ttgtagaata tccttcgcta ctcagtatgg tctggccatt
     6121 acctgcaagc aaactaaatt gaaactctcc atttttcctt gtcttaactt caaattttcc
     6181 catagattat tcttttgttt aaatgataaa aacgaaaaag atacaatatt atgacgacat
     6241 tgcattcata aagtcatcat ttatttcaaa acaagcttat catacttttg tttttattcg
     6301 ttcaaattgc ccggttttcc aaaatataat aaggggtaca tcccggttct ttccacatta
     6361 acaaatagtt atttataaat ttattgctca tataattatg attatcatgc gtttataaca
     6421 cataattcaa ttgtttaaat ttttttctcg tttttactgt accccttatc aaaataaatc
     6481 gtctaatagg gctgataaac aaaataatgt tttgaaacaa aaatactatt cataaagcaa
     6541 aattattttt gtcatgaaat cctactactt aaagaactct attatcctga tgttatttct
     6601 gatcacctct tccatctacc ttattgccca atgccctgaa ggagcgataa ctccaacacc
     6661 tggagcggcc ctgacaatca aatccggtga aatgtattgc atttcatccg atatttcatt
     6721 gctaagtgta tccattgaag agggtggcga gcttatactt gcgccaaaag taaccgttga
     6781 ggcgaatggc attttcaata ttgacggcta tctgaccctg tatgattatg ccggtttgaa
     6841 gctcacaggc gccatgaatc agggcagcag gtatcctgcc gatactaaga tttccatcgg
     6901 caacaacgcc ttcatctcca tgacaggatc ttttacacag tttgaccctg gagcgaatcc
     6961 tgcccatcct aatgcacagg ctaaaatccg actgagcaat aatgccgtaa tggagatttg
     7021 cggtacctat acacagttct ccggcaatta ccccgcagtg gattatgccg gtgatatgga
     7081 ccatgtcgct tacttcatca ccaaagctcc agcaagtggt gtcggctcat ccatactttc
     7141 cggggatgaa gaaactacct ggattgccat cggccaagtt gcaaacctca acccgggtga
     7201 tgcttcctac tgcgggccaa acgcttcacc ggataaatgc gacctatggc catcttggtt
     7261 atcagccgaa tattataatt gctttgatgc cgaaaataat cgagacataa gcctgcctat
     7321 tgttttggtc agttttgatg tagtgaagaa tggtttaata gcatccttga cctgggcgac
     7381 tgctgttgaa tacaaccata gtggctttga gattcaaagg agttcagacg gaataaattg
     7441 gtctaacctt ggcttcatcg ctgcttcaca aggctttggt gtcaatacag tcacgacacg
     7501 ctatgctttc gatgatttga atccactgcc gggcaacaat ttttacaggc taaaacaagt
     7561 ggatactgac ggtagcttct ctttcagccc ggtcagaact gttggtttta tggggaaaat
     7621 cgacaatctt caggtctatc ccaatccggt gaataatgtc gtgaatattg caggcgtcgc
     7681 ccctaacagt gtcatcaaaa tttttgatat ggcaggaaat caggttaacc gaaaattcac
     7741 tttatcagct atgaattctt ttgacttatc cgcctgtaaa gaaggtattt acttaatagt
     7801 ggtgagcgat ggttcaggaa atactgaaac attcaaaata ttgaaaaaca aggttcctta
     7861 accgaaattt taaatactac gactaatctg cccggcttat cgaaaagtag ccgggctttt
     7921 tttgattttt caagagtccc tgattaagga tcccagtaag acaaaagact atgattattt
     7981 aaagggctac atcatgtgtg gtttttccat gtccggcatt tatcttatct tcacccttct
     8041 aaaccgtaca caatgagcgg acttatagct ccttcgatac tctctgccga ctttaccaaa
     8101 ctggctgacg aaatctctat gatcaaccga agtgaagccg attggtttca tatcgatgtt
     8161 atggacggag tttttgtacc caatatcacc atgggctttc tcgtcgtcaa ggcaatcaac
     8221 caactggctg aaaagcccat ggatgtgcat ttgatgacag tgcacccgga attgtacatc
     8281 gaacaatggc gcgatgcagg agcggatcac atcaccgtac actacgaagc ctgcacacat
     8341 ctgcatcgtg tgattcagca gatcaaacat accggagcaa aggcaggagt cgccatcaat
     8401 ccgcacacgc cggtcaaact attggaggac atcatcgaag atctggatct tgtcatcgtc
     8461 atgtcggtca atcccggatt tggtggccag gccttcattc cacgcacctt tgacaaggtc
     8521 cgggcacttt cagccatgat cagggaacga aatgcatcca cccttataga gattgatgga
     8581 ggtgtcaccc ttgccaacgc cggtgcactg attgaatcag gcgctcatgc tctcgtggca
     8641 ggcaataccg tcttcaactc tcccaatcct acggagacga ttcatttatt aaaaaaaacc
     8701 tgctctcata ctaaacataa cgcatgatgt cgttagacag agagcttaat ccaatccgaa
     8761 tgccattgtt tatacgtctc ttttttcctg ttttcttcct cttgctttgt ttcaagttag
     8821 atgctcagga aacaagtact cttgttt
//
LOCUS       DAOQQN010000092         8805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3062_length_8805_cov_5.550057, whole genome shotgun sequence.
ACCESSION   DAOQQN010000092 DAOQQN010000000
VERSION     DAOQQN010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8805
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3062_length_8805_cov_5.550057"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PLG24_04945"
     CDS             complement(<1..713)
                     /locus_tag="PLG24_04945"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HMZ24269.1"
                     /translation="MLHGLLLYSQNRAIDAESGDGLPFCTIVSKHNPDIYTVSDINGN
                     FNLAHTVAGDTIIIRYIGYDTLEAIFLPNKEYRLYPATSMLKEAVIIADDGPAIALIK
                     KVLKRRNDHNILNLDYYQCRIYSRNTIGFDTSADSLDIKQFGKIFRYPASLFISESII
                     ERQYERKQKMYEKVITSKMSGLRDEKFVILPEDVQSLNFYRDYISIFNKDFVNPISPL
                     SWLKYHFNLDKVYEEANDT"
     gene            870..2303
                     /locus_tag="PLG24_04950"
     CDS             870..2303
                     /locus_tag="PLG24_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24270.1"
                     /translation="MYYIKRLFFLTLLSVTFITANAQQNQQVSQINAVNNYISFANES
                     DHGLLIIHRLLELYNQEMNKLVNLPHYRLNVFSNADFPENIFEDKSGSFYRVSPNELY
                     AKALKESSALSPKFSSKANEIIGNMKRILDDLEKERYSIGTLVANTDLKRISNLKNVY
                     AALDACVVKFDSYEAERKELAALIARMGPATRKNDSFYKAFVGPYNEYLQDLHQMFNL
                     LRLENTAEAVKAGQPLLKSAKKLSKELEDVEIGKLTKPQGLVVDELKMVLRKQISNLE
                     KFISNPTPPEEYELYGSSYYFYNIQLAGTTNKYGTGVVDRGNEWIKANGGNYIYGFER
                     PHFYKVIRPAAIQEEEKKVAVEHAPEVDENRKVVVDKEKSISIDVEKLLIEVYDNQEF
                     DHDTISLKFNGEWILQKQLITKEPIRIYLPLVKGQDNYLILYADNLGTIPPNTCAIRW
                     VNEKGKYNEVVLTSDYNTSEMIVIRRK"
     gene            complement(2540..2610)
                     /locus_tag="PLG24_04955"
     tRNA            complement(2540..2610)
                     /locus_tag="PLG24_04955"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2576..2578),aa:Gln,seq:ttg)
     gene            3095..3760
                     /gene="mnmD"
                     /locus_tag="PLG24_04960"
     CDS             3095..3760
                     /gene="mnmD"
                     /locus_tag="PLG24_04960"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HMZ24271.1"
                     /translation="MAEELIISEDLSPTLYSQDFNAHYHSLHGAMTETMVVFIDAGLN
                     FKLKEKAHINILEIGFGTGLNAFATLLNLPMDAEINYVTLEKFPLSGEITDDFIGKLN
                     LDQQAIALYQAIHACPWGDFQKIVPSFNLKKIRVDFLEFRTNYFFDLIYFDAFAPDTQ
                     PELWDGELFGYLYERLVNGGVLTTYCAKGIVKRKLKEIGFVIESLPGPPGKREITRAI
                     KQS"
     gene            3916..5388
                     /locus_tag="PLG24_04965"
     CDS             3916..5388
                     /locus_tag="PLG24_04965"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HMZ24272.1"
                     /translation="MNKILIANRGEIAVRVIKTCRKLGIKSVAVYSDADKLALHTTLA
                     DEGYHIGSNILSDSYLNAEKIIRVALESGAEAIHPGYGFLSENEDFARLVNDAGLIFI
                     GPGPDAIRTMGNKLAAKEAVKAFNIPMVPGTDGIVKDKIIAKFAAIDAGFPILIKAAA
                     GGGGKGMRTVYTESEFDEAYDRAVSEAVTAFKDGSVMIEKYVTKPRHIEIQIIADNHG
                     NVCHLFERECSIQRRHQKVIEEAPSSILSEAMRQSMGRAACDAARACNYSGVGTVEFL
                     VDDENNFYFLEMNTRLQVEHPVTELITGLDLVEQQIRIAKGEKLSFVQEDLKINGHAI
                     ELRLYAEDSFNNFLPSTGVLNEYIEPTGKGIRVDAGYRKGDEISVYFDPLIAKLIVWG
                     EDRNLAIQVMKAAIQQYQVSGLQTTLPFGAYVMNHPAFVTGNFDTGFVDKYYNSEEKA
                     ELEKSEAIVAAIVAEYLSKDRHSVRPVHIERPSAWRINTW"
     gene            complement(5553..6827)
                     /locus_tag="PLG24_04970"
     CDS             complement(5553..6827)
                     /locus_tag="PLG24_04970"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HMZ24273.1"
                     /translation="MSGKYIDVIIGLQWGDEGKGKIVDYLAEQYDIVCRFQGGPNAGH
                     TLKIDGNKFVLHTVPSGIFRKNIINIIGNGVVIDPITLVKELDNLKAAGIAYKDRLKI
                     SRKAHLILPSHRLLDLASESSKGREKIGSTLKGIGPTYMDKTGRNGLRMGDLADPDFL
                     GKYQKLKEKHLALARQYDEVDFDLDGEENKWMECISQIKELAQIDCEYYINSALESGK
                     KILAEGAQGSMLDIDHGTYPYVTSSNTITAGVCSGLGVAPNKIGEVIGITKAYCTRVG
                     SGPFPSELNDESGEKLRSEGHEFGATTGRPRRCGWIDLPALKYSVILNGVTQLIMTKL
                     DVLNSFEKVYAAIAYEIDGQSTDQVPFNMETINRLTYKEYKGWNSSLENIRETDNLPV
                     EAWVFITDIEKRLGVPFSIISTGPEREKLILR"
     gene            complement(6972..7916)
                     /locus_tag="PLG24_04975"
     CDS             complement(6972..7916)
                     /locus_tag="PLG24_04975"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HMZ24274.1"
                     /translation="MYPDLSYVFHDLFGTTPDNWLSLIKMFGLLLAIAILCSSYFLSL
                     EFKRRENEGSLHQLTVKKDGKTTKMWPHEKVGDITIIAAISGVVGAKIFAIFESIDSI
                     KSFLRDPIHTLFSGSGLAIYGGLIIAFIVVSRYVKRMGIKVLDMCDATAPSLMIGYGV
                     GRIGCQLSGDGDWGIVAGPKPDWFILPDWLWSQTYPRNVAEEGIKMAECAGKYCMELS
                     QGVFPTPIYETFFAFVIFGILWSLRKKLVISGQLFFLYCMLNGIERFFIEKIRVNDTF
                     DFGAFKASQAEIIAFILFLTGALGFYFVTWRSKKPIPE"
     gene            8141..>8805
                     /locus_tag="PLG24_04980"
     CDS             8141..>8805
                     /locus_tag="PLG24_04980"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HMZ24275.1"
                     /translation="MRYTVILLVVMVLTSACNPAKKAKVHETVVVVPPSRDTVLIEHE
                     PEKVNRSDSAQIRSLVEGINMDFQSALLTGSMVFENTTDRYNFNFNIRMVKDSVIWMQ
                     LKKIGLEGARMLIRRDSLFLVDRIHRTYLKKSWNEIRTQLNGPVDFDALYSILTGNVE
                     YFDKGMDTLKTSENMKWIYSSDGNNRTVIGINAVFNTLVSYMVQDLRKDNLIRIGLAN
                     HKI"
BASE COUNT         2626 a         1626 c         1870 g         2683 t
ORIGIN      
        1 aatgtgtcat tggcttcctc atagactttg tcgaggttaa aatgatactt taaccagcta
       61 agaggtgaaa tggggttgac aaagtcttta ttgaagatgg aaatataatc tctataaaaa
      121 ttgaggctct gtacatcttc cggaaggatt acaaactttt catcccgaag gcctgacatt
      181 ttagaggtaa tcactttttc atacatcttt tgtttccttt cgtattgccg ttcgattatt
      241 gattcggata tgaagagaga tgcaggatat cggaatattt tcccgaattg tttgatatcc
      301 aaagagtcgg cggaagtatc aaatccgatg gtattccttg agtaaatcct gcattgataa
      361 tagtccagat taagaatatt atgatcattc cgacgtttca aaaccttttt tatcagggcg
      421 atggccggac catcatcggc aattattaca gcttccttta gcatggaggt ggcaggatag
      481 aggcgatact ctttattagg cagaaatatg gcttccagcg tgtcataacc tatatatctt
      541 atgataatgg tatcaccagc tactgtatga gccagattga agtttccgtt gatatcggat
      601 accgtataga tatccggatt atgtttgctt acaattgtac aaaatgggag gccatctcca
      661 ctttctgcat cgattgccct gttctgactg tataataaca acccatgcag cattaacaat
      721 aacattacat tatatcttac gatacaatta aatagtgtat ttttgggcgt ggtcaaattc
      781 ctttgttata aatattggat tttggcaaat atatttatca aataatgatt taaagattat
      841 aaaactcgaa cattaaaaat taagttcccg tgtactacat aaaacgtcta ttctttctga
      901 cattattatc agttacattt attacggcta atgcgcagca aaatcagcaa gtttcacaga
      961 ttaatgcagt caacaactat atctcatttg ccaacgaaag tgaccatggg cttttgatta
     1021 tccaccgatt attggaatta tataatcagg agatgaacaa attggtcaat cttcctcatt
     1081 atcgactgaa tgtgttttca aatgctgatt ttccggaaaa tatctttgaa gataaaagtg
     1141 gatcatttta ccgggtcagt ccaaatgagc tttatgcaaa agcgctgaaa gaatcttccg
     1201 cattatctcc aaaattttcc tctaaggcta atgagatcat aggcaatatg aagaggatat
     1261 tggatgattt ggagaaagag cgatacagta tcggaacact tgtggctaat actgatctga
     1321 agagaatttc caacctgaag aatgtctatg cagctttgga tgcatgtgtg gtcaaattcg
     1381 attcttatga agccgagaga aaggaattgg cggctctgat tgccaggatg ggccctgcga
     1441 ccaggaagaa tgattcgttc tacaaggcat ttgtcggacc atacaatgaa tatttgcagg
     1501 atctgcacca aatgtttaat ctgctaaggt tagagaatac agcggaggca gtcaaagccg
     1561 gacagccctt gttgaaatca gcaaagaaat tgtctaaaga attagaggat gtcgagattg
     1621 gcaaattaac taaaccacaa gggcttgttg tggacgaatt aaagatggtc ctcagaaagc
     1681 aaatttccaa tttggaaaaa tttatatcta atccgacacc gccggaggag tatgagttgt
     1741 atggttcatc ttattatttt tacaacattc agttagccgg tacgaccaat aagtatggca
     1801 ccggtgttgt ggatcggggt aatgaatgga taaaggccaa tggtggcaat tatatctatg
     1861 gctttgaaag accgcatttt tataaggtta tcaggccggc agctattcag gaggaagaaa
     1921 agaaagttgc agtcgaacat gccccggaag tagatgaaaa tcgtaaggtt gtggttgata
     1981 aagaaaaatc aatcagtata gatgtcgaaa aattgctcat tgaagtgtat gacaatcaag
     2041 agtttgatca tgataccatc tcgcttaaat tcaatgggga gtggattctt caaaaacaac
     2101 tgattacaaa agaaccaatc cggatttatc tccctcttgt caaagggcaa gacaattatc
     2161 tgattctcta tgcagacaat cttggtacca ttccaccaaa tacctgtgct atacgctggg
     2221 tcaacgaaaa aggtaaatat aatgaagtcg tgttgacatc tgattacaac accagtgaga
     2281 tgattgttat tagaaggaag tagcagggat ggttatgaaa ggatcccggc cattgcatgg
     2341 aataaatagt ttagattggt ttgttaatat gataaatatt ggattatgtc tttctttaga
     2401 actttgtttg cagggatggc cgcatggaaa tggggtagtg gttgtatcgg tacaatctta
     2461 gttttcctga tcgcttattg gctattggga caacttggct gttgaaaata aaaaaagcct
     2521 tcttaggaag gcttttttgt tgaccgacaa ggattcgaac cttgacaaac agaaccaaaa
     2581 tctgttgtgc taccgttaca ccatcggtca ttttgctatt agcgggggca aagataagga
     2641 aggaaatata ttttgcaata cttttttgtg aaaaaattta ttgttttata acctgctgta
     2701 aatcaggcat atcaatgcgg cattgaggtc gtaggtttga atttatttta taattaaata
     2761 aattttcata gaatattgga acattctttt tcgtttggat attattgaat gcaaaataat
     2821 tgtaactttg ttctcttatt ataaaaattg taaccatgaa taaaaaggat tttttcaata
     2881 aaatttttgc agttttactt cttgcagttg ttgtttttgg atctacatct tgcaatcgag
     2941 gttatggatg tccttacaat acatctgcag gtgccggtat ttcgaagatt ctgaatttta
     3001 atttgttcaa ataatattaa acggcatact ggctttacag ctttatgtcg cagtaagcat
     3061 ataaaagccg gattgtccgg cttttctttt tgcaatggca gaagagttaa ttatatcgga
     3121 agacttatcg ccaacacttt actcccagga ttttaatgcc cattaccatt ctctgcatgg
     3181 tgccatgaca gagacgatgg ttgtattcat tgatgcaggg ctgaatttta agttgaagga
     3241 gaaggcacat attaatatct tggagatagg atttggcact ggcttgaatg catttgccac
     3301 cctgctgaat ttgccaatgg acgccgagat caattatgtt accctggaaa aatttccttt
     3361 atccggtgaa ataaccgatg actttattgg taaactgaat ctcgatcaac aagcaattgc
     3421 gctatatcaa gctatccatg cttgcccatg gggtgatttt caaaagattg ttccttcatt
     3481 taatttaaaa aagatccggg tcgattttct cgaattccgc acaaattact tttttgacct
     3541 gatctatttt gatgcatttg ctcccgatac acaacctgaa ctctgggatg gagagttgtt
     3601 tggctacctc tatgaaagac ttgtaaatgg tggcgtcctg acaacttatt gtgcaaaagg
     3661 aatcgttaaa aggaagttga aggaaattgg gtttgttata gaatccttgc cggggcctcc
     3721 gggcaagcgg gaaattacaa gggcgattaa acagtcctga atccttagtg ctggatcttg
     3781 ttggagacaa gtgtattggc gtattctgga ttgaggttat tgaatcagat tgaagggaag
     3841 gcaatattta tctgcagtaa aatgtatttt tctgtttcgg actatttata tttgagactt
     3901 tccaatgatt gaatcatgaa taaaattctt atagccaaca ggggcgagat tgccgttaga
     3961 gtaatcaaga cgtgtagaaa acttggaatc aaaagtgtcg cagtgtattc agatgctgac
     4021 aagctggcac tccacactac gcttgccgat gagggctatc atatcggaag taatatcttg
     4081 agtgattcgt acttaaacgc tgaaaaaatc atccgtgttg cgttggagtc cggggctgaa
     4141 gctattcatc cgggttatgg ttttctgagt gagaatgaag attttgcccg attggtcaat
     4201 gatgccgggt tgatttttat tggacccgga cctgatgcca ttcgtacaat gggtaacaaa
     4261 ttagctgcaa aagaagccgt caaggctttc aacattccca tggttcccgg cactgacggt
     4321 atcgtgaagg ataagataat tgctaaattt gctgccattg atgccggctt tccgatatta
     4381 atcaaagctg ctgcaggtgg tggtggaaaa ggtatgcgta ctgtttatac ggagagcgag
     4441 tttgatgaag cttatgacag agcggttagt gaagcagtga ctgccttcaa ggatggttcg
     4501 gtaatgatag agaagtatgt tactaaaccg agacatatcg agattcagat cattgcagat
     4561 aatcatggta atgtgtgcca tctttttgag cgggagtgct ccatccaacg gaggcatcag
     4621 aaagtaattg aggaagctcc cagcagcatc ctttcagagg cgatgcgcca atcaatgggc
     4681 agggctgcgt gtgatgctgc aagggcttgc aactacagtg gggtaggtac ggtagaattt
     4741 ctggtggatg acgaaaataa tttttacttt ttggaaatga acaccaggtt gcaggttgag
     4801 catccggtca cagaacttat aaccggtttg gatcttgtgg agcaacagat caggattgca
     4861 aaaggtgaaa aattgtcctt tgttcaggag gatttgaaaa tcaacggcca tgcaattgag
     4921 ctacgattat atgcggaaga ttcattcaac aatttcctgc cttcgacagg cgtacttaat
     4981 gagtatattg agccgaccgg taagggtata agagtggatg cgggatatcg gaaaggagat
     5041 gagatttcag tttatttcga tcctttgatt gccaaattga ttgtgtgggg cgaggatcgg
     5101 aatctggcga ttcaggtgat gaaagcggcg attcagcaat atcaggtaag cggcttacag
     5161 acaacacttc cctttggtgc ttacgtcatg aatcatccgg cttttgttac aggcaacttt
     5221 gacactggct ttgtcgataa gtattataat tctgaggaaa aagcggaatt ggaaaaaagt
     5281 gaagctattg ttgcagccat tgtggcagaa tatttgtcga aagatcgaca ttcggtcagg
     5341 ccggttcata ttgagaggcc ttcagcttgg cgtatcaata cctggtagga agattctaaa
     5401 gacattagat tggcacccgg tatagcggat gtatctttaa tagctgatca tagataaaat
     5461 ggaaaaacac aatcctcaga aatccaaaaa catgtataat tctgtcaagg catacttata
     5521 agtgcctatt tttgacagat tctgagtccc atttatctta aaatgagctt ttccctttcg
     5581 ggtcctgtag atatgatgga gaatgggacg cccagtcttt tttcaatatc agttataaaa
     5641 acccaggcct ctaccggcag gttatcggtc tccctaatgt tttcaaggga tgagttccag
     5701 cctttatatt ccttataagt tagccggttg atggtttcca tattaaaggg gacctggtcg
     5761 gtagattgcc cgtcaatttc gtaggcgatg gcagcgtata ctttttcaaa actgttcaac
     5821 acatccagtt tggtcataat cagctgagtt acgccgttga gtatgacact atatttcaga
     5881 gcaggcaggt ctatccagcc acatcttctg ggtcttccgg tggtagcccc gaactcgtgt
     5941 ccttcactgc gcaatttttc accagattca tcattcaatt cagaaggaaa ggggcctgat
     6001 ccgacacggg tacagtaagc ctttgttata cctatcactt caccgatctt attgggagca
     6061 acccccaatc cactacatac accggctgtt atcgtgttgg aagaagtgac gtaagggtaa
     6121 gtaccatgat cgatatccaa catagaaccc tgtgctccct cagcaagtat ttttttacca
     6181 ctttcaaggg cgctgttgat ataatattca caatcaatct gagcaagctc tttaatttga
     6241 ctgatgcatt ccatccattt attttcttcg ccatccagat caaaatctac ttcatcatac
     6301 tgtctggcta aggcaagatg cttttctttt aatttctgat atttacccaa aaagtccgga
     6361 tcggccaaat ctcccatcct tagaccgttt cgacctgttt tgtccatgta ggtcggcccg
     6421 atacctttga gcgttgatcc gattttctcc ctcccttttg aagattccga cgcaaggtcg
     6481 agcagacggt gactcggcaa tatgagatgc gccttcctgc taatcttcaa tcggtctttg
     6541 taggcgattc ctgccgcttt caaattgtcc agttccttaa ccagcgttat cggatcgatt
     6601 accacaccgt tgccgataat attgatgatg ttttttctga aaataccgct aggaaccgta
     6661 tgcaaaacga atttattgcc atcaatcttg agcgtatgac ctgcattcgg ccccccctga
     6721 aacctgcata caatatcata ttgttctgcg agatagtcga ctatctttcc cttaccttca
     6781 tctccccact gcagacctat gattacatca atatatttac cggacatgtc tcatgtttat
     6841 tatggacaaa aataacaaat attattactt ttcatcaact tatgttatac ggattgcaat
     6901 acggggtaag ttgatatata tatcatttac ttaatatatg tagatactta tttaacagat
     6961 agttggattt attattccgg aattggcttt ttactgcgcc aggttacgaa ataaaatcct
     7021 aaagctcctg tcaaaaacag gataaaggct attatttcag cctgggaagc tttgaatgcg
     7081 ccaaagtcga aggtatcgtt gacccggatt ttttcaataa agaaccgctc gattccgttg
     7141 agcatacaat aaagaaagaa taattggccg gatatgacca acttcttcct caatgaccaa
     7201 agaatgccga aaattacaaa agcaaaaaat gtctcataga taggagtagg aaatacaccc
     7261 tgagacaatt ccatacagta ttttccggca cattcagcca ttttaattcc ttcttcggct
     7321 acatttcttg ggtaagtctg agaccagagc cagtcaggaa gaataaacca atccggtttt
     7381 ggaccggcta caatacccca atctccatca ccgctcagtt gacatccgat acgccccaca
     7441 ccatatccga tcatcagtga aggggcagtc gcatcacaca tatcaagcac tttaatgccc
     7501 attcgtttga catatcgtga aaccacaata aaagcaatga ttagtccacc atagatagca
     7561 agaccacttc cggagaaaag tgtatgtatc ggatctctga gaaaagactt tattgaatca
     7621 atgctttcaa atattgcaaa aattttagca cccacaactc ctgatattgc agcgatgatt
     7681 gtgatatcgc cgaccttttc atgtggccac attttggtgg ttttaccatc ttttttcact
     7741 gtaagctgat ggagagaacc ctcgttttcc cttcttttga attccaaact caaaaaatag
     7801 gaactgcata gaattgcaat tgccagcaaa aggccaaaca ttttgatgag cgataaccag
     7861 ttatctggtg tcgtgccaaa taagtcatga aaaacgtagg ataaatccgg atacatagtt
     7921 attgttttta atcgtcagaa cgtcctcgag tcgtccatta aattgtaaag gtcaataaaa
     7981 tttatgattt ccttaatcca tatattgtaa tatttttcca actttggcga taataattct
     8041 gtagggttat tccgatggga attactcttt ctgattaaat accagatatt gaacggaaat
     8101 aatattatgg ttattgataa tgtattttaa aaccaatcct atgagataca cagttatatt
     8161 gcttgtggtg atggtcctga catcagcctg taatcctgcg aagaaagcca aggtacatga
     8221 gaccgttgtc gtcgtgcctc cctccaggga taccgtgttg attgagcatg agcctgaaaa
     8281 agtcaacagg tctgattctg cacagatccg aagtctggtg gaagggatca atatggattt
     8341 tcagtccgcc ttattgaccg ggagcatggt ttttgaaaac accaccgata gatataattt
     8401 caatttcaat atccggatgg taaaggatag tgtaatatgg atgcaattaa agaagatagg
     8461 acttgaaggc gccaggatgc ttataagaag ggattcctta tttttggttg atagaattca
     8521 tcggacttat ttaaagaaaa gttggaacga aatcaggacc caattaaacg gtcctgttga
     8581 ttttgatgca ctctattcaa tattgaccgg aaatgttgag tatttcgaca aaggcatgga
     8641 taccctgaag acttccgaaa atatgaaatg gatatattcc agtgatggga ataacaggac
     8701 tgtaattggt ataaatgccg tcttcaatac tttggtctca tatatggttc aggatttgag
     8761 aaaagacaat ttgataagaa ttggcttagc aaatcataaa atatt
//
LOCUS       DAOQQN010000093         8793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3064_length_8793_cov_4.957885, whole genome shotgun sequence.
ACCESSION   DAOQQN010000093 DAOQQN010000000
VERSION     DAOQQN010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8793
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3064_length_8793_cov_4.957885"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PLG24_04985"
     CDS             complement(<1..635)
                     /locus_tag="PLG24_04985"
                     /inference="COORDINATES: protein motif:HMM:NF014924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HMZ24276.1"
                     /translation="MRVFLSIWACLYFISLSAQPATISPFIKVDQFGYLCNSKKIAVI
                     SDPINGFNASESFSPGISTGAYQVRKWNGGDVIMSGTISPWNGGAVHTQSGDRGWYFD
                     FSSVTDPGIYYIWDNVNQVGSGQFFIGSNVYDANLRAVLRTYYYARCNYAKSMPYTEK
                     PEYNDVAAYEGSNQDKHARSSLDKNNPSTERDVSGGWFDAGDMNKYVTFAA"
     gene            828..1667
                     /locus_tag="PLG24_04990"
     CDS             828..1667
                     /locus_tag="PLG24_04990"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HMZ24277.1"
                     /translation="MTIIIVKSFLVIVLAWKLWEASKEGYQLSIDREFFTFLMIGFLA
                     ELVDGSLGMAYGVISSSFLIFFGVSPVVASAGVHTSEVFTTGVSGLSHLHFKNVDKGL
                     FFRIVIPGVIGSLIGAIVLSKLVDGEIIRPFIAVYLLLVGIKLIAREMQGDKVHIKPL
                     KSVPIIGFFGGALDAIGGGGWGPLVTSTILSRSASNAKHTIGTVNTAEFFVTFASTGI
                     FMFLIGISFFNILLGLIIGGVVAAPLGAYFLKHVPHKVLMYVVGSVLIIVSAFSIYYY
                     IFK"
     gene            complement(2124..2378)
                     /gene="rpmA"
                     /locus_tag="PLG24_04995"
     CDS             complement(2124..2378)
                     /gene="rpmA"
                     /locus_tag="PLG24_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HMZ24278.1"
                     /translation="MAHKKGVGSSDNGRDSKSKRLGVKKFGGQAVIAGNILVRQRGTR
                     FHPGVNVGCGKDHTLFALTSGVVAFKKGKKDRTFVDVVMN"
     gene            complement(2410..2721)
                     /gene="rplU"
                     /locus_tag="PLG24_05000"
     CDS             complement(2410..2721)
                     /gene="rplU"
                     /locus_tag="PLG24_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HMZ24279.1"
                     /translation="MFAIVSIAGQQFKVEGGQKIFVHRLDAKEGDKLTFDQVLLTSDG
                     NNVAVGTPTVSGVAVTATVLSEMVKGDKVIVFKKKRRKGYRVKNGHRQQFTHLQIDSI
                     G"
     gene            3034..3288
                     /locus_tag="PLG24_05005"
     CDS             3034..3288
                     /locus_tag="PLG24_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HMZ24280.1"
                     /translation="MKKDIHPSSYRKVVFKDISTDEAFITQSCVNTKDTIVWEDGNEY
                     PLVKVEISSFSHPFFTGKMKFVDTAGRIDKFNKKFAKFMK"
     gene            3384..4553
                     /locus_tag="PLG24_05010"
     CDS             3384..4553
                     /locus_tag="PLG24_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HMZ24281.1"
                     /translation="MKIVLYDGPEWKDLLPLTFTKPVAGLRIGIDTIREKWEAALNTE
                     CLIKTQDYLQAIHPVATSDDFLFINASYIPHPVLTNHILSIRHNQSLAISGQVIAYRT
                     DSFHVASSEAKQFEIISINAPEDVIHICYPWDIFSNNNRVLQQDFDRITSGRVSAPIS
                     PTNRLLGERIFLEEGAIVECCNINTTTGPVYLGKNSMIMEGANIRGGLALCEESIVKM
                     GAKLYGASTFGPHCKVGGEVNNSIFQGFSNKGHEGYLGNSVIGEWCNFGADTNASNLK
                     NTYKKVKIYNYRQNISIDSGLLFLGLIMGDHSKSGINTMFNTGTVVGVFANIFGTGFP
                     PGHIPSFSWGGAEHMEPYQFEKAIEVAEAVMSRKNQHLTPEYKAILRHIYDNTHH"
     gene            4568..5260
                     /locus_tag="PLG24_05015"
     CDS             4568..5260
                     /locus_tag="PLG24_05015"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HMZ24282.1"
                     /translation="MKLPVNTWTKLLSYLVKVPVSRSESINDGALYVDLVKGRLQLST
                     DHAVYSFDDKYDNFVKALKAVKADLFRNKSILVLGGGLGSIPYILEKNHQFQGELTMV
                     ENDPAVIRLFLEYSLPRMNTLIDIRESDAIIFMEQNSRKYDMIIVDLFIDDTVPDQFL
                     EADFLMLCKVNLVKGGLVLFNWMTVTKAQTQNYKDYRDMIFNEIMDHPLEVKTKYNHI
                     LVGYSFPTISGS"
     gene            5747..8653
                     /locus_tag="PLG24_05020"
     CDS             5747..8653
                     /locus_tag="PLG24_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HMZ24283.1"
                     /translation="MLRYFSILLLFLSIIPLYAQKQSFVSGQVMIMLQDNTGFEDFTR
                     ELYSNNRNVLIDNYKQLSKTIPIYLISSQSFEGDEKVWVSFIAKLSTVRFAQVNHYVT
                     LRNTVPQDPRYSDQWQWNNTGTGNGKIGADVSAEKAWDITTGGLTAAGDTIVVCVIDD
                     GIDLQHEDLAANIWYNNGEIPNNGIDDDGNGFVDDFNGWNFNTQNDNVSVGSHGVNVS
                     GMIGAVGNNNMGVTGINWNVKIMNIVLSDFTEAAIVECYDYPYQSRLAYNNSHGDKGA
                     FVVSVNMSNGIDYGNPDDAPIWCGFYDIMGEAGILNVAATSNQNINVDVDGDLPTGCT
                     SQYLISVGRTDKNDNYAPCGYGPISIDLGAPGVNIVTTRSRNRYTTTTGTSFSSPLVA
                     GLVGLMYSYPCDNIGQLMHDDYAAFALLVKDAILNGVDKISGYESKCLAGGRSNAYQS
                     LQQMELLCAACQPGSVTSLDYTSGNAYIVQFNNGDNQQNIRFRPSGTDDWTIMENVVS
                     PLHLELPIACTEYEFQMQTICPDDTSAWGFSKILKSAKCCESFDDLKIFVDSTGIVTI
                     VNADLDQDVKSEIHYKIDGDATYTTTTVTGDTVRLSSLLPCTFYKIYVSVLCNNGNHV
                     VSDSMRIFSACLSDCGPKVCVPTANAQQGFIDDFTINGTTASTGDNHGYIDFGDHLNL
                     RAPDVGPFLNIVNINRKQSGTVRIRMFIDFDKDGIFSNGELFWEQSMQGNTKHLEYTH
                     NFYNAIQPGTYKARLMMIFNPSEGPCEGGSGEVEDYCLTVFHVVPNECAAVETIRTIS
                     KSYTSIELGWDKPQDDVIAYVYRYKELPSGDYNYFSDTAHKIILKNLKECTNYEFGVF
                     TLCQTDSSDYKVINFKTDCTNASNQLDHSIHWKVFPNPFGNKINVLLTSNTYNEGVLR
                     LVDTKGHTVYHEKMSLSIGDHYINLDVDGNLPPGMYILTLTDGRGVKSAKLIKQ"
BASE COUNT         2688 a         1831 c         1730 g         2544 t
ORIGIN      
        1 aatgcggcga aggtgacata cttattcata tcaccggcat caaaccatcc accactgaca
       61 tccctttctg ttgaagggtt gtttttatcc aaactgcttc tagcatgctt gtcctgattg
      121 cttccttcat aagcagctac atcattatac tccggctttt cagtatatgg catcgatttg
      181 gcatagttac agcgggcata ataataagta cggagcactg ccctcagatt ggcatcatac
      241 acattggaac cgatgaagaa ctggccactt cctacttgat tgacattgtc ccatatataa
      301 taaataccgg gatcagtaac tgacgaaaaa tcgaaatacc agccccggtc gcccgattgg
      361 gtatgtacag ccccaccatt ccacggcgaa atagtcccgg acattataac atctccacca
      421 ttccattttc tgacttgata ggcacctgtt gaaatgcccg gactaaagga ctctgacgca
      481 ttgaatccat tgatgggatc tgaaatgacg gctatctttt ttgagttaca tagataacca
      541 aactgatcca ccttgataaa tgggctgatg gtggcaggct gggcagacaa ggatataaag
      601 taaaggcatg cccatattga tagaaaaact ctcatggcta caaataaaat taggaacaaa
      661 aaacacacac gataaaaaag gggcgccgac aaagataaag aacattctcc ttcaacttaa
      721 ttttttttaa aaaattttat tcaatcgctg atggaaaaaa tatcatttat tagttttacc
      781 aaaaaagaag atatatgttt caaaatatca ccaacaaaca aatttccatg acaattataa
      841 ttgtcaaaag ttttttggtg attgtattgg cctggaagct ttgggaggca tcaaaagaag
      901 gatatcaact ttccatcgat agggagtttt ttactttttt aatgattgga tttcttgctg
      961 aattagttga tggctctttg gggatggctt atggagtcat cagttccagt ttcctgattt
     1021 tttttggtgt ttcaccggta gtcgcctcag caggtgtaca tacttcagaa gtattcacaa
     1081 ccggtgtctc tggtctttca catctgcact ttaaaaatgt ggataaagga cttttcttcc
     1141 ggattgtcat tcccggagtg attggctcat taattggagc gattgtttta tccaaactgg
     1201 tagatggaga gatcatccga cctttcatag cagtgtatct tttacttgta ggtatcaaat
     1261 tgatagcacg ggagatgcaa ggcgacaagg tccatatcaa accccttaag tcagtgccca
     1321 ttattggttt tttcggaggt gccttagatg caatcggagg aggaggatgg ggcccgcttg
     1381 tcacttctac tatattaagc aggagcgcct cgaatgccaa acacactatt ggcacggtca
     1441 acacagcaga attttttgtt actttcgcca gtaccggtat atttatgttc ctgattggaa
     1501 tttctttctt taatattttg ctgggcctca ttattggtgg tgtggtggca gccccgctcg
     1561 gcgcatactt cctgaagcat gttcctcaca aagtactgat gtatgtggtg ggctcggtgt
     1621 taattatagt ttctgcattt tccatctatt attatatctt taaataagag aagacaattg
     1681 tcagaaaata aatcaggaat ctcaacagga tacggaaaga ggtatgaaca atgttaccag
     1741 aaatgttaag ctcatctcca aataatattg caggtgtcag caattgaaat ccttacctat
     1801 ggaatagcca gaaattaaca ccttgctttt gttttgctaa aaggaaacat ttttcttatt
     1861 aagctataaa tatttacaat aacccggaca attgccatcc ggacatttca tgttcttcgt
     1921 tgagaagcaa aacctactta gcgttggaaa atatgatctt tgttctacga aattcagagg
     1981 cagtaaggac tttagaatgt ggagtcctcc tcaactttca ggaactatga gaaattgtaa
     2041 tgggtgcaag cagatacaag tggtataaaa aaaatcccgg aagctatggc caccgggatt
     2101 ctgtatctaa taaaagtcag gaatcaattc atcactacat cgacaaaagt cctgtctttc
     2161 tttcctttct tgaatgctac aacaccggaa gtgagtgcga aaagagtgtg atccttaccg
     2221 catccgacat taacaccggg atggaatctg gtaccacgct gacgtacgag gatattacct
     2281 gcgattacag cctgtcctcc aaatttctta acacccaatc ttttgctttt actgtcccgt
     2341 ccgttatctg aactaccgac accttttttg tgagccattt cttaattttt aaatgattaa
     2401 taaaaaagat tacccgattg agtcaatctg gagatgagtg aattgctgtc tgtggccatt
     2461 cttgacacgg tatccctttc ttcttttttt cttgaagacg ataaccttat ctcccttgac
     2521 catttctgaa agtacggttg cagtaactgc cacaccactt acagtaggag taccgactgc
     2581 cacattattg ccatcacttg tcaaaagcac ctggtcaaat gtaagcttgt cgccctcctt
     2641 tgcatccaac cggtggacga agatcttctg acctccttca actttgaact gctgaccggc
     2701 tatagaaaca atagcgaaca tagttatttg atttaatttt tgataaaagt ggggcaaagg
     2761 taaacattat tatctattca ggcaagaatt tttatgcatt tcatttcacg ttctgaaaaa
     2821 taaatacata aagcaacagt ttcccttgta ttacaaacat tccccggcaa tcatccggat
     2881 gttttacagc aaaccaaaac catccaaatt ccatccggac aaaaattcca atccttcaca
     2941 ccataaaaaa gttgaatatt tttttataaa attttgaata tttcaatata tactttacct
     3001 ttgcacccgc aaatcaataa atctgataac aacatgaaaa aggacataca tccttccagc
     3061 tatagaaagg tagttttcaa ggacatctct acggatgagg ccttcattac tcaatcatgc
     3121 gtcaatacca aagacacaat cgtttgggaa gacggtaacg aatatccact ggtaaaggtg
     3181 gaaatatctt cattctcaca cccgttcttc acaggaaaga tgaagtttgt tgacaccgca
     3241 ggacgtatcg ataagttcaa caagaagttt gcaaaattca tgaaataagc tgattctttc
     3301 agtccagaat atcaaaaagc catgtatccc atggcttttt ttatttgatt tttctatctt
     3361 gcatcatcaa aagtccgcaa taaatgaaaa tagtccttta tgacggtcct gaatggaaag
     3421 acctattgcc cctcactttt acaaagcccg ttgccggcct aaggataggc attgatacca
     3481 ttcgggaaaa atgggaagcg gcactgaata ccgaatgtct catcaagaca caggactact
     3541 tgcaggctat tcacccggtg gcaacctctg atgattttct attcatcaat gcatcttaca
     3601 tcccccaccc tgtcctgact aatcatattt tgtcgatcag acataaccaa agtttggcta
     3661 tcagcggaca ggtcatagct tatcgcactg actcatttca tgtagcgtct tccgaggcaa
     3721 aacaatttga aattataagt attaacgcac ctgaggatgt tatacacata tgttatccat
     3781 gggatatttt ttccaacaac aaccgggtcc tgcaacaaga ttttgacaga ataacgtcag
     3841 gtagggtttc ggcgcctatt tctcccacca atagattgct tggagaaaga atatttctgg
     3901 aagaaggtgc cattgtagaa tgttgtaata taaatacaac aaccgggcct gtataccttg
     3961 gcaaaaattc catgattatg gaaggggcga atatacgtgg aggtctggca ctttgtgaag
     4021 aaagtatcgt aaaaatgggt gcaaagttat acggtgctag tacattcgga cctcactgca
     4081 aggttggcgg agaagtcaat aacagtatct tccagggctt ttccaacaaa ggccacgaag
     4141 gctatctcgg caacagtgtt atcggcgaat ggtgcaactt tggtgcagat accaatgcat
     4201 ccaatttgaa aaacacctac aaaaaggtca agatttacaa ctaccgacaa aatatttcca
     4261 ttgactccgg acttttattc cttggtttga tcatgggcga tcattctaag tccggcatca
     4321 atactatgtt caacaccggg actgtggtag gcgtatttgc caatatcttt ggtacgggat
     4381 tcccgcccgg ccatatccct tctttctcgt ggggtggagc tgaacatatg gaaccttatc
     4441 agtttgaaaa ggcaatagaa gtagctgaag cagtcatgag caggaaaaac caacatctta
     4501 ctcctgaata caaggcaatc ttacgccata tttacgacaa tacgcatcac taaatatatt
     4561 catcgaattg aaattacccg tcaatacctg gaccaaactc ctaagttatc ttgtaaaggt
     4621 gcccgtgagc aggtctgaat ccataaatga cggcgcactg tatgttgatc tggtcaaggg
     4681 acgtctccag ctcagtacgg atcatgcagt atactccttt gatgacaaat acgacaactt
     4741 tgtcaaagcc ttgaaagccg tcaaggcgga tcttttccgt aacaaatcca ttcttgtcct
     4801 gggcggaggt ttaggtagca tcccttatat tttggagaag aaccatcaat ttcaaggtga
     4861 actgaccatg gtggaaaatg atcctgctgt gattagatta tttctggaat atagtctgcc
     4921 aagaatgaat acccttattg acatcaggga atcggatgcc atcatcttta tggaacaaaa
     4981 ttcaaggaag tacgacatga taattgttga tttgtttatc gacgatactg taccggatca
     5041 atttcttgaa gcagatttcc ttatgttgtg taaagtcaac ctcgtcaaag ggggcctggt
     5101 cctattcaac tggatgactg ttactaaagc gcagactcaa aattataagg attacaggga
     5161 catgattttc aatgaaataa tggatcatcc cttagaagta aaaacaaaat acaatcatat
     5221 tcttgtcggt tattctttcc ccacaatttc aggcagttaa cccgcaattc cccggtattt
     5281 tatggttaag agccatcact gaggttgcct taactctgat aattatctca ctttgggatt
     5341 tccgggaagt ataaattcca aaagccaata atatcatcat caacaatgcg cccatccatt
     5401 taattatcct tgtattcacc atgcctcagg atttgaacta cccataccga tattctgtct
     5461 ctatttactt aatgcactgt aaaaaccaca tgcttattct caaattatca gacaaactaa
     5521 ccgagatctt attcaaaata ttgcactttg aaccaaaaaa aatcaaaatt taacactttt
     5581 aagtaagtcg atataaggta taactttggt tttttggtaa tagccgattg atcattagga
     5641 tttttcccaa attgaaaaaa tttccaaaaa ccatgtatat ttgagagttt ataaccataa
     5701 aagtataaaa tataggtctt tggccacatt cataaaaacc agttctatgc ttagatattt
     5761 ttccattcta cttctctttt tgtccataat tcctctgtat gcccaaaaac aatcctttgt
     5821 ctccgggcaa gttatgatta tgcttcagga taatactgga tttgaagatt ttacccggga
     5881 attatattcc aataatcgga atgtgctcat cgataattat aagcaattat ccaagaccat
     5941 ccccatctat ctgatctcat cacagtcatt cgaaggtgat gaaaaagtct gggtttcatt
     6001 catagccaag ctttcaactg tcagattcgc tcaggtcaat cattacgtaa cacttagaaa
     6061 cactgtcccg caggatccaa gatatagtga tcaatggcaa tggaataata ccggtaccgg
     6121 aaatggtaaa attggtgcag acgtaagcgc cgaaaaagca tgggacatca caactggcgg
     6181 ccttactgcc gcaggcgata ccatcgtagt atgtgttatt gacgatggta ttgaccttca
     6241 gcatgaagac cttgcagcta acatatggta caacaatggc gaaatcccaa ataacggaat
     6301 cgatgatgat ggcaatggct ttgtggatga tttcaatggc tggaatttta atactcaaaa
     6361 tgacaacgta agtgtaggca gtcacggggt gaatgtgagt ggaatgattg gagcggtagg
     6421 aaataataat atgggagtta caggcatcaa ctggaacgta aaaattatga acattgtcct
     6481 ttcagatttt actgaagccg ccatcgtaga gtgttatgac tatccgtacc aaagcagatt
     6541 ggcttataac aactctcatg gagataaagg tgcctttgtg gtttcagtca atatgtccaa
     6601 tggtatcgat tatggcaatc ctgatgatgc accgatttgg tgtggcttct atgatatcat
     6661 gggagaagcc ggaattttga atgtagccgc cacatccaat caaaatatca atgtcgatgt
     6721 ggacggagat ctgccgaccg gttgtaccag tcaatacttg atatctgtag ggcgtaccga
     6781 caaaaacgac aattatgcac cctgcggcta cggcccgata agcatcgatc tgggtgcccc
     6841 gggggtcaat atcgttacta cgagatcaag aaacagatat actacaacaa caggaacttc
     6901 tttttcttcg ccattggtgg cgggcctcgt aggcttaatg tatagttatc cctgtgacaa
     6961 cataggtcag ttgatgcacg atgactatgc tgcctttgca ctacttgtca aggatgccat
     7021 cctcaatggg gtagataaaa tttcaggcta tgagagcaaa tgtctcgccg gaggtcgttc
     7081 caatgcatat caatcccttc aacagatgga attactttgt gcagcatgtc aacccggaag
     7141 tgtaacttct ctggattata ccagcggcaa tgcttatatt gttcaattca acaatggaga
     7201 taaccaacag aatattcgat tccgaccaag cggtactgat gactggacaa tcatggaaaa
     7261 tgtcgtatcc ccattgcatt tggaattgcc tattgcctgc acagaatatg aattccaaat
     7321 gcagacaatc tgtcccgatg atacttcagc ctggggcttc agtaaaatcc tgaaatctgc
     7381 caaatgctgt gaatcttttg acgatttaaa aatattcgtt gattccaccg gtatagttac
     7441 tatagtcaat gctgatctcg atcaagatgt aaaaagtgag attcattata aaatagatgg
     7501 tgatgcaacc tatacaacta caactgtgac cggagatact gtcaggctct cgtctctgct
     7561 accttgtaca ttctataaga tatatgtcag cgtgctttgc aacaatggca atcatgtcgt
     7621 gagtgattcc atgcgtatct tttctgcctg cctttctgac tgtggcccta aggtatgcgt
     7681 acccaccgca aatgcgcaac agggtttcat cgatgatttt accatcaatg gaacgactgc
     7741 aagtacagga gacaatcatg gatacattga ctttggagat catcttaatc tgagagctcc
     7801 tgatgtcggg ccatttctga atattgtaaa catcaacaga aaacaatcag ggacggtccg
     7861 tatcagaatg tttatcgatt ttgacaaaga cggtatcttc tccaatggag agctcttttg
     7921 ggagcaatct atgcaaggca ataccaaaca tttggaatat acccataatt tttacaacgc
     7981 catacaaccc ggaacataca aagcaaggct gatgatgata ttcaaccctt cagaaggacc
     8041 ttgtgagggc ggctccggag aggtggaaga ctactgcctt actgttttcc atgtagtgcc
     8101 aaatgagtgt gcagccgttg aaacaattcg cacaatttcc aaatcctata cctcgattga
     8161 gctgggctgg gataagcctc aggatgatgt aatagcttat gtataccggt ataaggaact
     8221 tccttcagga gattacaact acttctcaga tacagcacac aagattattt taaagaacct
     8281 taaagaatgt accaattatg agtttggtgt ctttacactc tgccaaacgg attcaagtga
     8341 ttataaagta atcaacttca agaccgattg taccaatgcc agcaatcaac tggatcattc
     8401 aattcactgg aaggtattcc ccaatccatt tggaaataaa atcaatgttc tcctgacttc
     8461 caatacatat aatgaaggcg tgttgcgcct tgtcgatacg aaaggacata cggtgtacca
     8521 tgaaaaaatg tccctgtcca ttggagacca ttatatcaat ctcgatgttg acggaaattt
     8581 accgcccggg atgtacatcc tgaccctgac tgatggcagg ggggtaaaat cagcgaaatt
     8641 gattaaacaa taaaacaaaa ggttgttcca cggagcaacc ttttttaatt agcgactcac
     8701 agagcaggca tcagggaagc gatcccgcaa taggaataat caaccagcaa ggttaattcc
     8761 gataatttaa gcaggaaaaa tacgctttaa gct
//
LOCUS       DAOQQN010000094         8769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3073_length_8769_cov_6.447211, whole genome shotgun sequence.
ACCESSION   DAOQQN010000094 DAOQQN010000000
VERSION     DAOQQN010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8769
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3073_length_8769_cov_6.447211"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..2341)
                     /locus_tag="PLG24_05025"
     CDS             complement(164..2341)
                     /locus_tag="PLG24_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ24284.1"
                     /translation="MRFLQTVILQIIIMLPALAQHHQDTVKKHTLDPVIIGGMKSTSD
                     IRRLNKVEGTYIISGKKNEVIELNNQNNGLAEKYCRQIFAKIPGIFVYDMDGTGNQVN
                     ISTRGLDPHRGWEFNIRKDGIITNTDLYGYPASHYNVPYEAVERIELVRGTSAQQYGG
                     QFGGMLNYITKQPDSRKIGFESINTVGSFGLLSTYNSISGTIGKFRYAAWMNKKAVDG
                     YRRFNDSKYNAESLALYYDFSENIKLKLEYTHSNYITHLAGALTDKEFKEDPTQATRT
                     RNYYQPDIHIPNLTFDWKISDHTSLRYTSNAILGARNSVMFDKPANVRDTIVTATGQY
                     NNRQVDIDNYHTFLNELRMVHEYQFFNLKNTITSGIQVTNNDLHRRQQGVGTTGTDYT
                     LTLVKPGWGRDLHFKTGNIAVFVENSWAITDRLSFNTAARMEYGVSRLSGKINLYPED
                     ELPNTIDHKFPIFGASFQYNLDKSNIYGGWSQAYRPVILKDIVPGSLYEKADKNLRDA
                     FGYNAELGYRGSAGWLQWDIDAFYLLYRHRLGTFAQVDADNNLIIYRTNIGDSHTKGI
                     EAFIQGDWTLGKFMTLSLFNATSLMDARYKNATIRSGSENISVDGNKVESVPTVITRS
                     GATLKYNRWTYCIKYSYTAESFADALNTITPSANGATGIVPAYSLVDMNLSVRVNDKL
                     RLQANVNNVLDESYFTKRPQFYPGPGIWPSDGRSFSVTASIKI"
     gene            complement(2677..4641)
                     /locus_tag="PLG24_05030"
     CDS             complement(2677..4641)
                     /locus_tag="PLG24_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HMZ24285.1"
                     /translation="MPLKSVLFTVFCFLYQFSYGQHIARGDFRNVLLLEAPILHEKDV
                     AVAVFSDLGAWHAYRFHRDQNGKIGFSGPLIMGMEGRWVGKYISRPRIFIDGTEKAFL
                     PDQITGTAYPGMMEIKYTSSDIAIHQRLIFVSNRQSMILTEITNLTHKKLKVEPCLEG
                     KFLESGRLTKRDREIYFDVGTTPGFTLRLDRDASIRILDHQYVIVFDTQSMKPGEKLS
                     YVESHIYSPVDSEHIHTINTESATFQQSEATNEYRWNNYLNTYFANAGELPAEKRRLA
                     VKAIITLITNWRSAAGDLHHDGLFPSVSYQGFYGFWSWDSWKQAVGLALFNPALAKSN
                     ILSMFDYMDDFGMVADCIYLDKSENNYRDTKPPLAAWAVESIFTRSGDTSFVQSIYPR
                     LLKYHHWWYKNRDHDGNGLCEFGSTDGTRLAAAWESGMDNAVRFDSANMVRNNTHAWS
                     LDQESVDLNAFLYAEKLCLANLADIIGLNEDAQQLRKESRWLITKIRQYLFDSTTGYF
                     YDKKLNEHHLIKVAGPEGWLPLWARCADDEQARSVVHVVLDTARFNTFLPFPTLDASH
                     PEFNPAKGYWRGPVWTDQLYFGLKGLRNYGYWNDANKLQEKFFNHAEGLTTNKPLYEN
                     YHPLTGVGLNAMNFSWTAAHILMLLVEDSP"
     gene            complement(4733..5515)
                     /locus_tag="PLG24_05035"
     CDS             complement(4733..5515)
                     /locus_tag="PLG24_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMZ24286.1"
                     /translation="MLLLESPHNDKFKKLLRLVSDNSFRRKQGVFVVEGLKEIEAAIV
                     FGYEVEDIYLDVKYRDKFDPAGFGHIRTTLLSHALFSRLAYREDTSIVIATVREPELN
                     LEDLTLSDNPLIVILEEVEKPGNIGAILRTCDAAGVDAVIACQQQHNFFHPNVIRSSV
                     GTVFSTQLGCGGPEDILQWCKNHGIHIYSAALQTDTYYTSADYTQPTAFVMGSEDKGL
                     KNFWRKTSDGIIKIPMLGHNDSLNVSVSAAILLYEARRQRGF"
     gene            complement(5736..6428)
                     /locus_tag="PLG24_05040"
     CDS             complement(5736..6428)
                     /locus_tag="PLG24_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24287.1"
                     /translation="MKPKRCLLFTCLIVGFLFFNACNKEDINTNTGSWSGQALNADTL
                     YFDNETQFRDFLFHYGLSRKYEIIPPKYHFSCTVVPLPNNYPEQMRDDYLNEDRFVGK
                     PFINLFDHIATRKYINIFFPTYYENNPGTTKVLNFCDVNEYITNLATGQVVCFKDVFN
                     WYDENGENIFNANKKVWLLWAYNDAVVKFGIEIVHVPTGVDYTFKEYYPAHNELDLTL
                     SDIAIKYGHLKP"
     gene            6598..8166
                     /locus_tag="PLG24_05045"
     CDS             6598..8166
                     /locus_tag="PLG24_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HMZ24288.1"
                     /translation="MPVRIKNLAAHHHHPWFPITFVSMSIMKKLAGETIYYGISSIFG
                     RFINFFLTPIYTYSVIIDKAKMGHLTELMAYTAIILILFTCRMEAAYFRFGKDKDNQE
                     AAYRNSVTTVVSLSVFFGLLLFMLSPIIAPVLDYGGEHIYFQLLAVIMTIDAFCELPL
                     SRLRFEGKAKKFAFIRIINILTNVCFNIFFVVVCPRLYDAGYSFVTAWFDPEKIIVYI
                     FLSQVFGSLSSLILLSKYLKGVGRVDIPLLKRMVAYSWPLIIVSLVSMFNEMFGRVIL
                     KWFLTGSLSDNEAQLGVYGANYRLATIIALFTQAFRYAAEPFFFKESNNVGAEKTYAD
                     VTKYYLIFSIYGFLFVSLYIDIFKYFVGPKYFEGLKVVPILLLANVFNGLYFNVSIWY
                     RLKDKTLTGAMIAIFGAVLTLFFNWYLIPILGYIGSAIATLVCYSSMTIICFVLGQKV
                     MKVPYQVRTMLLWMGAAILIQIGIWYLRSLDISVYLVLLCSTIGIVLFSGLIWSYEKE
                     KIKTLLRSIKLRSR"
     gene            complement(8195..>8769)
                     /locus_tag="PLG24_05050"
     CDS             complement(8195..>8769)
                     /locus_tag="PLG24_05050"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HMZ24289.1"
                     /translation="DYIAILPGSRHQEVKRILPVMVGAMAQMPQKKFVIARTSLLPED
                     EFTFILDQIHPDWKKNVSIVTDKTYDTIYHASSAMVTSGTATLETALLNTPQVVCYAA
                     NSLNIRIAKAFVKVKYISLVNLIPDMPIVQELIQEDLSVSNLTREILKLEDDADYRNK
                     MLEAYGLLKTLVGEPGVANRMALDILHRIG"
BASE COUNT         2308 a         1901 c         1970 g         2590 t
ORIGIN      
        1 aacgtccgca tgaatcattt actaaaaaac gacaccactc aggtgctgct ttcatttaat
       61 accggcctcc ggtaatcttt ttttgaccga atattaaatg aaagcggcaa aaatgctacg
      121 tcccggctaa ttgtacttcg tccggccgag atcggacata ggattatatt tttatacttg
      181 cagtaaccga aaatgatctc ccgtcagaag gccaaatacc cgggccggga taaaactgag
      241 gcctcttggt aaagtaggat tcatcgagga cgttgttgac attggcctgg agtcgtaatt
      301 tatcattgac acggacactg agattcatat ccaccaggga ataagccgga acaatacctg
      361 tagcgccatt ggccgatgga gtgattgtgt tgagcgcatc agcaaagctc tcagcggtat
      421 agctgtactt gatacaatat gtccaacggt tatatttcag ggtggcgcca ctgcgcgtta
      481 ttacagtcgg gacactttcc actttattac cgtctactga gatattttcg ctaccggatc
      541 ggatcgttgc attcttgtat cgggcatcca ttagggacgt tgcattgaac aaagacaatg
      601 tcataaattt gcccagggtc cagtctccct gaataaatgc ttctatacct tttgtgtggg
      661 aatcacctat gttggttcta tagataatca gattattatc tgcatccact tgagcaaatg
      721 ttcccaaccg atgcctgtac aacaggtaga aggcgtcaat gtcccactgg agccatccgg
      781 ccgaacccct atatccgagt tcagcattat atccgaaagc gtcacgcagg tttttgtctg
      841 ccttttcata tagggatccg ggaacgatat ctttcagaat gaccggccga taggcttggg
      901 accagccgcc atagatattg cttttatcga gattgtattg aaatgatgct ccgaaaatcg
      961 ggaatttgtg atcaattgta ttgggcagct catcctccgg atataaattg atcttgccgg
     1021 acaatcggga cacaccatat tccatccttg ctgccgtgtt gaaggagagg cgatcggtta
     1081 tggcccagct gttttcgaca aatactgcaa tattgccggt cttgaaatgc aggtcccgtc
     1141 cccagcccgg cttgaccagg gtaagtgtat aatcggttcc tgtagtgccg acgccctgtt
     1201 gtcgacgatg gaggtcattg tttgtgacct gtatgccgga agtaatagta ttcttcagat
     1261 taaaaaattg atattcgtgc accatcctca gctcattcaa gaaagtatgg taattgtcaa
     1321 tatctacctg tcggttgttg tattgtccgg ttgctgtcac gatggtgtct ctcacattgg
     1381 ccggtttgtc aaacatgaca ctgtttctgg cgcccaatat ggcattggaa gtatagcgga
     1441 gactcgtatg gtcgcttatt ttccaatcga aggtcaagtt ggggatatgg atatccggtt
     1501 gatagtaatt gcgagtcctg gtagcctggg tgggatcctc cttaaactcc ttgtccgtca
     1561 atgctcctgc aagatgtgta atatagttgg aatgcgtgta ttccagcttt agctttatat
     1621 tctcactgaa gtcgtaatac aaggccaaag actctgcgtt atatttggag tcgttgaacc
     1681 tacgataacc gtccaccgcc tttttattca tccaggcagc atatcgaaac ttaccgattg
     1741 tcccactgat ggaattatag gtactcaata gaccgaatga tcctacggta ttgatggatt
     1801 caaatccaat cttgcggctg tcaggctgtt tggttatata attgagcatg ccgccaaatt
     1861 gcccgccata ttgttgggca gaagtgccgc gcaccagctc gattctttcc accgcctcat
     1921 aaggcacatt gtaatggctc gcaggataac catacaagtc agtgttggtg atgatgccgt
     1981 ccttgcggat gttgaactcc cagccacggt gagggtccag tcctcgagtg gagatattga
     2041 cctgattgcc ggtgccgtcc atatcataga caaaaatccc gggtatcttg gcaaatattt
     2101 gacgacaata tttttctgcc aggccattgt tctgattatt cagttcgatg acttcgttct
     2161 ttttcccgga aatgatatag gtaccttcga ctttattgag tcggcgtatg tcacttgttg
     2221 acttcattcc tccaatgatg acaggatcga gtgtatgctt cttgaccgta tcttgatgat
     2281 gttgtgcaag agcgggaagc atgattatga tttgaaggat tacggtttgt aaaaatctca
     2341 tgaagtgaaa aatctgattg attgcaaaag taaggttgac cttcccggca aaaaaacgca
     2401 ccggttaaat ttaatttatt tttaatggta tccccaattt cagattcgta tttatccggt
     2461 caaataggtg tctcaaaagc agattggttg atgccacata aatatcaatc aacaatgaca
     2521 gtaactttgg attgggggag atcagtgatt gattcaaaag gaagtgatta ttgaaatatg
     2581 aagacggaga accatctgcg tcagtgaatt gtgggaaaat gattttcctg ccgccttctc
     2641 tttgtcgctg aaagaagcca ttcccggcct gatgcctcaa gggctgtctt ccaccagcag
     2701 cattaaaata tgtgcagctg tccaactgaa attcatggca ttaagaccca ctccggtcaa
     2761 gggatggtag ttttcataca agggcttgtt ggtcgtcaaa ccttctgcat ggttgaaaaa
     2821 cttctcttgc agtttatttg cgtcattcca atacccataa ttacgcaatc cttttaaccc
     2881 gaaatacaat tggtcggtcc atacgggacc tcgccagtat ccttttgccg gattgaattc
     2941 cggatgcgaa gcatcgaggg ttggaaaagg aagaaaggta ttgaatcttg ctgtgtccag
     3001 cacaacatgg accacagatc gcgcctgctc gtcgtcggcg catcgtgccc ataaagggag
     3061 ccagccttcc ggtccggcaa cctttatcaa gtggtgttcg ttcaattttt tatcataaaa
     3121 atagcctgtc gtactgtcaa agagatattg tctgatctta gtgataagcc acctgctttc
     3181 cttccttaat tgttgagcat cttcgttcag gccgattata tcagcaagat tggcaagaca
     3241 caatttttca gcgtatagaa aagcattgag gtccacactt tcctgatcga gtgaccatgc
     3301 gtgtgtgttg ttccggacca tgttagcgga atcaaagcgc accgcattgt ccataccgct
     3361 ctcccaggct gcagcgagcc ttgtcccatc cgtcgatccg aactcacaaa ggccattgcc
     3421 atcgtgatcc cggtttttgt accaccagtg atgatatttc aaaagtcggg gatagatcga
     3481 ctgtacaaat gaagtgtcac cggacctcgt gaatattgac tccacagccc atgctgcgag
     3541 tggcggcttt gtatcccggt aattattttc cgacttatcc agatatatgc aatcggctac
     3601 cattccgaaa tcatccatat aatcgaacat gctcagaata ttgctttttg ccagagccgg
     3661 gttaaacaag gcaagcccga ccgcctgttt ccaggagtcc caactccaga aaccatagaa
     3721 tccctgatag gaaacagatg gaaagaggcc atcgtggtgc aggtctcccg ctgccgaccg
     3781 ccagttggtt atcaatgtga ttatggcctt gaccgccaga cgcctctttt cagctggcag
     3841 ttctcctgca tttgcaaaat aggtgttcag gtagttattc cagcgatact cattggttgc
     3901 ttcggactgt tgaaatgtcg ctgattctgt atttatcgtg tgaatgtgtt cactatcaac
     3961 agggctgtaa atatgagatt cgacatagga aagtttttct cccggcttca tggattgcgt
     4021 atcgaaaaca atgacatatt gatggtccag gatccgtatg gaggcgtccc gatccaaacg
     4081 gagtgtaaat ccgggtgtag tgccgacgtc aaaatatatt tcgcggtctc tttttgtcaa
     4141 gcgccccgat tcaaggaact ttccttccag acacggttcc actttcaact ttttatgggt
     4201 caggttagtg atttcagtta agatcatcga ttggcggttg ctgacaaata tcaatcgctg
     4261 atggattgcg atatcagatg aggtatattt tatctccatc ataccgggat aggcggtccc
     4321 tgttatttga tccggtaaaa atgccttttc tgtaccatcg atgaatatcc ggggccggga
     4381 gatgtatttg ccgacccatc gaccctccat gcccataatc aaaggcccgg aaaaacctat
     4441 ctttccattc tgatcccgat gaaaacgata ggcatgccat gcccccagat cactgaaaac
     4501 agccactgca acatcttttt catgcaggat aggggcctcg agtagcaaga catttcggaa
     4561 gtcccctctg gctatatgct gtccgtatga gaattgatat aggaaacaaa aaacggtgaa
     4621 gagaactgat ttcaaaggca taaactaaag aatataaaac caaagataaa gatgtttccg
     4681 gagaaaactg attcgggaag tgctttatca atcagcggga aggcgttagt gatcaaaatc
     4741 ctcgctgcct gcgagcttcg taaagcaata ttgctgcact caccgataca ttgagcgaat
     4801 cgttatgtcc cagcattgga atctttatga tgccgtcaga agttttacgc cagaaatttt
     4861 tcaggccctt gtcctcagag cccatgacaa aagcagttgg ttgtgtgtaa tcggcagagg
     4921 tatagtatgt atccgtctgc agtgcggcag aataaatatg gatgccatgg tttttacacc
     4981 actgcaggat atcctcaggg ccaccacatc caagttgcgt tgagaatacc gtgccgacac
     5041 tggaacgaat tacattagga tggaaaaagt tgtgctgttg ctgacacgct atgacggcat
     5101 ccactcctgc cgcatcacag gtgcgaagga tggcgccgat atttcccggc ttttctactt
     5161 cttccaggat gacgataagg ggattgtccg acaatgtcag gtcttccagg ttgagctccg
     5221 gctccctgac ggtggcaatc acgatgctgg tatcttcgcg atatgccaac cgactaaaca
     5281 gggcatgact cagcagtgtt gttctgatat ggccaaatcc tgccggatca aacttatccc
     5341 tatatttaac atccagataa atatcttcca cttcatatcc aaacactatg gccgcctcga
     5401 tttccttcaa gccttcaacg acaaaaacac cttgcttccg ccggaaggaa ttgtcagaaa
     5461 ccaggcgtaa cagtttttta aatttgtcat tatgtgggct ctccagcaaa agcatctcca
     5521 ataaattaag atgcaaggta aagaagttca gacaaattgt tgagtagcat atgcggaaat
     5581 aaagttatca caaacaggag gaagccggaa atagttacat aatagtaagt gtattaaatt
     5641 atttcaggtg aagctgaaaa cggagcaggt ctgtatttta aagcatgatg acatatttta
     5701 aaaatcatta ccaacaaaaa agttgcttgt tggaatcaag gcttgagatg tccgtacttt
     5761 atggcaatat cagacaaggt aaggtccagt tcattgtgag cgggataata ttccttaaaa
     5821 gtatagtcta ctccggtagg cacatgcact atttcgatgc caaatttcac tacagcatcg
     5881 ttgtatgccc aaagaagcca caccttcttg tttgcattga agatattttc accattttca
     5941 tcataccagt taaagacatc tttaaaacag acaacctgac ctgttgcaag attagtgata
     6001 tactcattaa catcacaaaa attaaggacc ttggttgtgc cgggattgtt ttcgtaataa
     6061 gtcggaaaaa aaatattgat gtatttccgg gtggcaatat gatcgaatag gttgataaag
     6121 ggcttgccga caaatctatc ctcattgaga taatcgtcac gcatctgctc aggataatta
     6181 ttgggaagag ggacaacagt acacgaaaaa tgatattttg ggggaatgat ctcatatttc
     6241 cgggaaagac cataatgaaa aagaaagtcc ctgaattgag tttcgttgtc aaaatacagc
     6301 gtgtctgcat tcagggcctg acctgaccag ctaccggtat tcgtattaat atcttcctta
     6361 ttacaggcgt taaaaaacag aaatccgaca atcagacagg taaaaaggag gcatcgtttc
     6421 ggtttcatat cgttgctttt gtagtttgga tggccatttt gctaaagagt ttaattcact
     6481 ttgttgaaaa aataaattat taatcgttga aaggattgct atctcaagat aggttgacta
     6541 ctcaacgtat taggcaatag aaaggctttc agtctttttg ggggagggtg ttttaatatg
     6601 cctgtacgta ttaaaaactt agccgcacac caccatcatc catggtttcc tattaccttt
     6661 gtgtccatgt ccatcatgaa aaaactggca ggagagacca tttactatgg tatcagcagc
     6721 atctttggcc gctttatcaa ttttttcctg acgcccatct atacttactc cgttatcatt
     6781 gacaaagcta aaatgggaca tctgacggaa ttgatggcct ataccgcgat aatcctcatt
     6841 ctcttcacat gcaggatgga ggcggcctat tttcgattcg ggaaggacaa agacaatcag
     6901 gaggcggcct atcgcaactc ggtaaccacg gtggtttcat tgtccgtttt ctttggatta
     6961 ttgctattca tgctgtctcc gatcattgcc ccggtcctgg attacggcgg agagcacatt
     7021 tattttcagc tgcttgccgt catcatgaca attgacgcct tctgtgaatt accgctttcc
     7081 cgtttgcggt tcgaagggaa ggccaaaaaa ttcgcattta ttcgcatcat taatatcctg
     7141 accaatgtat gcttcaatat tttctttgtc gttgtatgtc cgcggttgta tgatgcggga
     7201 tattcctttg ttacagcttg gtttgaccct gaaaagatca ttgtttatat tttcctgagt
     7261 caggtctttg gctctctttc ttcattgatt ttactgagta aatatctaaa aggtgtaggc
     7321 cgggtcgaca ttccattgtt gaagaggatg gttgcttatt cctggccatt aattatcgtc
     7381 agcctggtat ctatgttcaa tgaaatgttt ggtcgtgtta tcctgaagtg gttccttacc
     7441 gggtctttat cggacaatga agcccagctg ggcgtatatg gcgccaatta tcggctggcg
     7501 acgatcatag ccttatttac ccaggcattc agatatgctg ccgagccatt cttttttaag
     7561 gaatccaata atgtgggcgc tgaaaaaacc tatgccgatg tgacaaaata ctatctgatc
     7621 ttcagtatct atggctttct ctttgttagt ctgtatattg atatcttcaa atattttgtc
     7681 ggccccaaat attttgaggg attaaaagtg gtgccgattc tgcttcttgc caatgtattc
     7741 aatggccttt acttcaatgt gtccatttgg tacaggctta aggacaagac attgaccggt
     7801 gcgatgattg cgattttcgg agctgttttg acgcttttct tcaactggta tctgattcct
     7861 attctcggtt atatcggttc tgctattgcc acactggtct gttattcttc catgaccatc
     7921 atttgctttg tactgggcca gaaggtgatg aaggttcctt atcaggtcag gaccatgctc
     7981 ctttggatgg gggcagccat attgatacag attggcattt ggtatttgag aagtttggat
     8041 atttcggtat accttgtgtt gttatgttca accatcggta ttgtattgtt ttccggcctg
     8101 atctggagtt atgaaaagga aaaaatcaag accctgcttc ggtctatcaa gcttaggagc
     8161 cgatagtaag aagtaatttg tggataaatg agacctatcc tatccggtgc aggatatcca
     8221 gtgccatcct gtttgccaca ccgggttcgc cgacgagtgt ctttaataat ccgtaagctt
     8281 ccagcatttt gttgcgataa tcggcgtcgt cttccagttt cagtatttcc cttgtgagat
     8341 ttgacacgga aagatcttcc tggataagtt cctgtactat aggcatatcg ggaatcaagt
     8401 tgaccaatga aatatatttg accttgacaa atgcctttgc tatgcgaata ttcagactgt
     8461 tggcggcgta gcagactacc tggggcgtgt tgagcaaggc agtttccagc gtcgccgtac
     8521 cgctggtaac catagccgag gatgcatggt aaatggtatc gtaggtttta tcggtgacga
     8581 tcgatacatt tttcttccaa tcaggatgaa tttggtccag gatgaaagtg aattcatctt
     8641 caggcaataa ggaagtcctg gctattacaa attttttttg aggcatctga gccatggccc
     8701 cgaccattac gggcaggatt cttttcactt cctggtgtct actgcccggc aatatggcaa
     8761 tataatccc
//
LOCUS       DAOQQN010000095         8749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3085_length_8749_cov_5.738095, whole genome shotgun sequence.
ACCESSION   DAOQQN010000095 DAOQQN010000000
VERSION     DAOQQN010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8749
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3085_length_8749_cov_5.738095"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..819
                     /locus_tag="PLG24_05055"
     CDS             178..819
                     /locus_tag="PLG24_05055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HMZ24290.1"
                     /translation="MVELYKSASPIVTVIMVTYNRAEYLDRSIGSFLNQTFKNTELLV
                     IDDGSEDNTFEVVLKYMMAHDNIRYFRHSNRNISLSKNVGLKAAAGRYITFLDSDDAL
                     KPDYLESRVDYMEHHPEMDMIEGGVIIIGNPYVKDRNNPERLIHLSECHIGATYFGKT
                     EVFLSSGGYDKSITYSEDSQFWTKVEKKYKTGKFDYPGYIYYRDTPGSVCNTV"
     gene            877..1386
                     /locus_tag="PLG24_05060"
     CDS             877..1386
                     /locus_tag="PLG24_05060"
                     /inference="COORDINATES: protein motif:HMM:NF017856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HMZ24291.1"
                     /translation="MEGTSDVFTVENYLYFTRLIIGVVLCYLLYINFPELPFQWSLVS
                     VVVAISPDNSTQLAKDRMIANLLGCVVGLVMFFIHPPNLLMLSIGVMLVVIIGLYFNM
                     QGSIRSALAALVILMLYNNQSADWTLALDRLVCVIAGCLVALLVTLAFNYLFKVCRFK
                     SPQPKDQAT"
     gene            complement(1401..2786)
                     /locus_tag="PLG24_05065"
     CDS             complement(1401..2786)
                     /locus_tag="PLG24_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HMZ24292.1"
                     /translation="MDTNSQAEEILSRITDDQDFFKALRSKEYSRLDTPVHQIYLDFT
                     GGNLYPSSLIKKHHDALLNGVFGNPHSINPTSARSSILVESAREKVIEFFNAEEYLCV
                     FTPNATGALKIVGECYPFTSDSHYVLLSDNHNSVNGIREFCKNHGGSVNYVPVQYEDL
                     QINDEILKKALTDNAGQSNSLFSFPAQSNVSGVKHSLDWIKFAQNLGYDVLLDAAAFV
                     PTSRLNLKEVQPEFVSISFYKIFGYPTGLGCLLIKKSVFDKLKKPWFAGGTVTMVSVT
                     SQHSFLAGNHERFEDGTLNYLDIPALQNGLEFIESIGMERINARIKGLTQYLLDNLHA
                     LHHKNGKPLVKVFGPHDMEQRGGNLVMNFFDENGNTIDFESIDVVARDKNISIRSGCF
                     CNPGIDEINNCITNDEMAQYFTSRDKGDYHDMVKYLGKMRGATRVSVGIATSKEDLDA
                     FIEFLKEHFKI"
     gene            2906..4195
                     /locus_tag="PLG24_05070"
     CDS             2906..4195
                     /locus_tag="PLG24_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HMZ24293.1"
                     /translation="MIRLQPFIYKECSFRFDALKGLMEKLKNKRLAILSLGLLSALGP
                     FSIDMYLPGFNDMAHSLKTTVPEIGLSLSSFFVGISIGQLIYGPLLDRYGRKKPLYIG
                     LVIYFFTTLACTMVKNVDQLIVLRFFEALGSCAGMVAARALVRDLFPVNKIAKVFSYL
                     MLVIALSPVLAPTFGGYISTWFGWPSIFMTLAGIGLLTLIVSWLWLPDGNRPDDEKSL
                     RPLPIIRSYLEVAGVPQFYIFASISAISFSGLYAYISGSADLFLNVYHVSQKEYGLIF
                     AIISLGMTFTGQLNTLFLNKFTSEQISFTALIIQAFLATVLLVLITWNVIGLYSMITM
                     ICVYLAALGFVFPNTSALCLTAFSRNAGTASALMGSVQLGIGAIVAGIVNMIPNHNGR
                     PMAMIMLICTLFSLAIFIYSKKTNLLKSGEKTIFLDV"
     gene            complement(4213..4713)
                     /locus_tag="PLG24_05075"
     CDS             complement(4213..4713)
                     /locus_tag="PLG24_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ24294.1"
                     /translation="MLLRRATEKDADRIWEIIQAAIEKRRAEGSMQWQDGYPNPQSIA
                     TDIEQGYGYVLADGESILAYTAIIFDKEPAYEVPNVDWLNGESYVVIHRVATASEVRG
                     SGKAILLFQMAEKVALNKGIFNIRVDTNFDNQPMLHILDKLGYKYCGKVYLRGGERLA
                     YQKILL"
     gene            complement(4679..5896)
                     /locus_tag="PLG24_05080"
     CDS             complement(4679..5896)
                     /locus_tag="PLG24_05080"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HMZ24295.1"
                     /translation="MRFCLLLIISFFGWMSSVDAQEEFPVFISGQENYTSYRIPAIIS
                     MPDGRLLAFAEGRVHHTGDFGDVNIVLKISRDRGKTWDNLRTIVDYDELHAGNPAPVI
                     DMADPNYPNGRIFLFYNTGNNTEYDIRKGNGYREVWYITSEDGGETWTQAVNITTQVH
                     RPQQPEVNPDYDFQENWRAYANTPGHALQFSWGKYRGRIYVPAYHSSGDLQFDARDYH
                     AHGYYTDDHGKTFQVSQPISLGGGNEATAAQFSEEGLMMNVRNQNGSVKARFIALSND
                     GGETWYREYFDRNLPDPVCEGSLLNVNRRVIAFCNNADTSGRNNLTLRLSTNRGESWF
                     QKTLIYSNDKVNDASGYSDIVNVNDRTIGVLYEKDNYSSIVFTTVEWSIPVYKALPKK
                     KSHVVKKSNRKRR"
     gene            complement(5943..6428)
                     /gene="purE"
                     /locus_tag="PLG24_05085"
     CDS             complement(5943..6428)
                     /gene="purE"
                     /locus_tag="PLG24_05085"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HMZ24296.1"
                     /translation="MNEVTPLASIIMGSDSDWPVMSDAAKILEELGIPHEKKVVSAHR
                     TPKMMFEFAENAYSRGIRVIIAGAGGAAHLPGMVASLSCLPVIGVPIKSSNSIDGWDS
                     ILSILQMPGGIPVATVALNGAKNAGILAAQILGTSNDEIYDRIAKYKAELEANVLAKK
                     L"
     gene            complement(6425..7561)
                     /locus_tag="PLG24_05090"
     CDS             complement(6425..7561)
                     /locus_tag="PLG24_05090"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HMZ24297.1"
                     /translation="MKKIGILGGGQLGRMLQAEAQQRNMPVYCMDKSENAPASLYRDF
                     YTIGDIRNYDDVMSFGADKDVITVEIEHINLEALRDLEKAGKEVYPNPDALEIIKNKN
                     IQKSFYHSIGIPIPEFSSFNSKDEAVIVFEQWKNRLPCIYKSAEMGYDGKGVRTIRAI
                     DDVLALDDIPGAFEQRIDIDIEIAVMIARSPDGNYVLYPPLAMYFDPENHILSEVYYP
                     ANMGKNIVEEMNRIAVKVTETLNICGLCAFEFFITSSGEVVLNEIAPRPHNSMHISMN
                     NAHCSQFEQHLRAISGLPLGSSVMEKAGIMYNIIGEEDDYGTAEWTGWHEVMNLHEVY
                     VHLYGKTEIKPARKMGHINIVGDDLSKLKEKLLTIRQYFKNRKS"
     gene            complement(7580..8206)
                     /locus_tag="PLG24_05095"
     CDS             complement(7580..8206)
                     /locus_tag="PLG24_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HMZ24298.1"
                     /translation="MYKPYIVGITGGSGSGKTTFLKSLSEGYSAEEVCILSMDNYYKP
                     RTQQVYDENGVQNFDLVESIDTQAFFNDIIRLVSGETIVQEEYTFNNSLADVEMITIK
                     PAPVMILEGLFIMHMKDLRSLLDLKIYIEAKDALKIIRRIKRDQSERNYPLEDVLYRY
                     ESHVLPSYEKYIFPYKNDVDIIINNNGNTDAVLNILRNHFDVIINSNQ"
     gene            8448..>8749
                     /locus_tag="PLG24_05100"
     CDS             8448..>8749
                     /locus_tag="PLG24_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HMZ24299.1"
                     /translation="MEKLNIAGELSADNIRMLISGMQRRLEKIYKGGGEKKIEKIKAE
                     NRLPARERVSMLLDSDRPSIEIGAFAGYEMYPEYGGCPAGGVIIFLGYVHGRICLVV"
BASE COUNT         2433 a         1745 c         1706 g         2865 t
ORIGIN      
        1 cataaatttt tggtatactt gtccggatag ctatgctact agagctctgc tctgtacagg
       61 gagtgaaaca atccggggtt ccgggatgcc gatgttacag tcgtatagat caatgaaatt
      121 ggtcaataac gaatgtacct tcggtattat ttccgaatgt gaaataaaat gaatgaaatg
      181 gttgaattgt acaaaagcgc ctcccctatc gttactgtaa ttatggtgac ttataacagg
      241 gcggaatacc tcgatcgaag tatcggatct tttctcaatc aaactttcaa aaatacggag
      301 ttgctggtta ttgacgacgg aagtgaagac aatacttttg aagttgtttt gaagtatatg
      361 atggcacatg ataatattag atatttccgc catagtaacc gcaatatctc actgtcaaag
      421 aatgttggcc tgaaagcagc tgccggtcga tatataacct tccttgatag tgatgacgca
      481 ctaaaacctg actacctgga gtccagggtc gattatatgg aacatcatcc ggaaatggat
      541 atgatagaag gtggagttat tattatcggg aatccatatg tcaaggaccg gaacaatccg
      601 gagcgattga ttcacttgtc agaatgccat attggcgcca cttattttgg taaaacggaa
      661 gttttccttt cttcgggtgg ttatgataaa tcaattacct atagtgaaga cagtcagttc
      721 tggactaaag tggaaaagaa gtacaagact ggaaaatttg attatccggg ttatatctat
      781 tatcgcgata ctccgggaag tgtatgtaat actgtgtgat atgcatatat atcaatttga
      841 ttgttttata tattaaataa aatataaata taacgaatgg aaggtacatc cgatgttttc
      901 acagttgaaa attacttgta tttcactcgc cttataattg gggttgtatt atgttacctt
      961 ctttacatta attttcctga gttgcctttt cagtggtctt tggtttcagt tgttgtcgct
     1021 atttcgccgg ataacagtac tcaattggca aaagacagga tgattgccaa tttattaggc
     1081 tgtgtggtag ggctggttat gtttttcatt catcctccga acctgttgat gttaagtatc
     1141 ggagtgatgt tggtggtaat tataggccta tatttcaata tgcaaggctc tattcggagt
     1201 gctttagccg cacttgtgat tttgatgcta tacaacaatc agtcagcaga ttggacactg
     1261 gccctggatc gtttggtatg tgtgattgcc ggatgtctag tcgcattatt ggtaactttg
     1321 gcatttaact acctatttaa ggtttgtcgg ttcaaatcac ctcaaccgaa ggaccaggca
     1381 acctgatttt tacagtgata tcaaatcttg aaatgttcct taaggaattc aatgaaagca
     1441 tctaaatcct ctttcgaagt ggcaatacct acagaaaccc tagttgcacc gcgcatttta
     1501 cccaaatatt taaccatgtc atgataatca cctttatctc tgctggtaaa gtattgagcc
     1561 atttcatcat tggtaataca gttgttgatc tcatcaattc caggattaca aaaacaacct
     1621 gatcggatgg agatattttt atcccttgct accacatcaa tgctttcaaa atcaatagta
     1681 ttgccatttt catcgaagaa attcattacc aggttgccac cgcgttgctc catatcatgc
     1741 ggtccaaaga ccttgaccaa gggtttgcca tttttatggt gcagtgcatg taaattatct
     1801 aataggtatt gagtcagacc ttttatcctg gcattgattc tctccatacc gatagattca
     1861 atgaattcaa gaccattttg gagggcagga atatcgaggt aattcagtgt gccatcttca
     1921 aagcgctcgt gattgccggc taaaaaactg tgctgagaag taactgatac catagttaca
     1981 gtgcctccgg cgaaccaggg ctttttcaac ttatcgaaaa cagacttttt aattaagaga
     2041 catcctaatc ccgtcggata tccgaaaatc ttataaaatg aaatactgac aaattccggt
     2101 tgcacttcct tcagattcaa tctggatgtt ggtacaaatg ctgcggcatc aagaagtaca
     2161 tcgtagccaa gattttgggc aaatttaatc cagtcaagcg agtgtttcac accacttaca
     2221 ttactctgag ccggaaaaga gaagaggcta ttgctttgtc cggcattatc ggtcaaagct
     2281 tttttgagga tttcatcatt aatttgcaaa tcttcatatt ggaccggaac ataattcact
     2341 gagcctccat ggtttttgca aaattctctg attccattga ctgaattatg attatcagaa
     2401 aggagtacgt aatgactgtc agaggtaaat ggataacatt cacctacaat tttgagtgca
     2461 ccggtagcat ttggagtaaa aacacataaa tattcctcgg cattgaaaaa ttcaataact
     2521 ttttctctgg cgctttcaac cagaatggaa gatcttgctg atgttggatt gatggaatgt
     2581 ggattgccaa agactccatt taataatgca tcatgatgct ttttgatcag agacgaagga
     2641 tataaattgc ccccagtaaa gtcaaggtag atctgatgga caggcgtatc cagtctggaa
     2701 tattctttac tcctcagggc tttaaaaaaa tcttgatcgt ccgtaattct tgaaagtatt
     2761 tcttctgctt gagaattggt gtccatagta ttttagaatt accacaaaat tagcaatatt
     2821 tggatataat atcaatatac attattacga tagaataatt ccccattgtc ttcagtattc
     2881 cataagttaa tttaccggta ttgcgatgat tcgtttgcaa ccttttatct acaaagaatg
     2941 ttcattcaga ttcgatgcac tcaaaggatt gatggaaaaa ttaaagaata aaaggctggc
     3001 tattttgtca ttggggcttc tttcagcatt ggggccattt tccatcgaca tgtatttacc
     3061 cggcttcaat gatatggctc attcattgaa aactaccgtg ccagagattg ggctgagtct
     3121 gtccagtttt tttgtcggaa tctctatagg tcaattgatt tatggtccat tgcttgaccg
     3181 ttatggaagg aagaaaccgc tttatatagg tcttgtcatc tactttttta caactttggc
     3241 atgtacaatg gttaaaaatg tagaccagtt gatcgtatta cgattcttcg aggcattagg
     3301 tagttgtgca ggcatggtgg ctgcacgtgc acttgtcaga gatctttttc cggttaataa
     3361 gatagcaaag gtattttctt atctgatgtt agtgattgcg ctctctccgg ttttggctcc
     3421 tacattcggt ggctatattt ccacctggtt tggttggcca tccattttca tgacgcttgc
     3481 aggtattggc ctactgacat taattgtttc atggctttgg ctacctgatg gtaacagacc
     3541 cgatgatgag aaatctctca gaccattacc gattataagg tcttacctgg aagtcgccgg
     3601 tgtcccgcag ttctatatct ttgcttccat ttcagccatt tcattttcag gtctctatgc
     3661 ctatatttct ggttctgcag atttattttt aaatgtatat catgtatctc agaaagaata
     3721 tggtttgata tttgctatta tttcccttgg aatgaccttt accgggcaat tgaatacctt
     3781 attcctgaat aaattcacca gtgaacaaat ttcgtttaca gccctcatca tacaggcttt
     3841 tcttgcaaca gtattgttgg ttttaattac atggaatgtt attggattat actcaatgat
     3901 tactatgatt tgcgtatatc tggcagcact tggatttgtt tttcccaata cttccgcatt
     3961 gtgtttaact gcgtttagcc gaaatgccgg tactgcttct gcacttatgg gatctgtaca
     4021 acttggtatt ggcgctattg ttgcaggcat agttaatatg attccaaatc acaacgggag
     4081 gccaatggcc atgattatgt tgatttgtac gcttttttct cttgctatct ttatatatag
     4141 taagaaaaca aatcttttga aatcaggaga aaagactatt tttttagatg tctaaaaaaa
     4201 aatttataac tctcataaga gaattttttg ataagccagt ctttctcctc ctcttaaata
     4261 taccttccca cagtacttat aacccagttt atccaggatg tgcaacatgg gttgattgtc
     4321 aaaattggtg tctacccgga tattaaatat tcctttgttt aaggcaactt tttctgccat
     4381 ttgaaacaac aggattgcct taccacttcc tctgacctct gaggctgtgg ccaccctgtg
     4441 aatgacaaca taagattcac cattaagcca atcgacattt ggtacttcgt aggcaggttc
     4501 tttatcgaag attattgcag tataggcaag gatggattct ccatcagcaa ggacataacc
     4561 atatccttgt tcaatatctg tagcaatgga ttgtggatta ggatatccat cctgccattg
     4621 catgctacct tctgctctgc gtttttctat ggcggcctgt atgatctccc agatgcgatc
     4681 agcgtctttt tctgttgctc ttcttaacaa catgactttt ctttttagga agtgctttat
     4741 atacgggaat cgaccactct acggttgtaa atacgatact ggaataatta tccttttcgt
     4801 atagaacacc aatagtgcga tcattcacat ttactatatc ggaatatcct gaggcatcat
     4861 ttaccttgtc attactataa atcaacgtct tttgaaacca ggattcgcct ctgttggtgc
     4921 ttagtctgag tgtcaggttg ttacgcccgg aagtatctgc attattgcaa aaagcaatga
     4981 cccgcctgtt gacattgagc aacgagcctt cacacaccgg atccggtaga tttctgtcaa
     5041 agtattctct ataccatgtt tcacctccgt cattacttag tgcaatgaag cgcgctttga
     5101 cggacccatt ctgatttctg acattcatca tgagaccctc ttctgaaaac tgggctgcag
     5161 tggcttcatt tccaccgccc agtgaaatcg gttgacttac ctggaaggtt ttaccatgat
     5221 catctgtata atagccatgt gcgtggtagt ctcttgcatc gaattgcagg tcgccggagg
     5281 aatgataggc aggtacatat atcctgccgc gatatttacc ccaggaaaat tgcagggcat
     5341 gacccggtgt attggcatag gcacgccagt tttcctgaaa atcatagtcc ggatttactt
     5401 caggttgttg tggtctatgg acttgtgtgg ttatattgac agcttgtgtc caggtttctc
     5461 ctccatcctc agaggtgata taccatactt cccgataacc attacccttc ctgatatcgt
     5521 actcggtatt gtttccggta ttgtaaaata gaaaaatcct gccattggga taattcggat
     5581 cagccatatc tatcacaggt gcaggattcc ctgcatgtaa ttcatcataa tcgacgattg
     5641 tcctaagatt gtcccatgtc tttcctctgt ccctactgat tttaagaacg atgttgacat
     5701 caccgaagtc gcctgtatgg tgtaccctac cttccgcaaa agccagtaat cggccatctg
     5761 gcattgagat tatggccgga atcctataac ttgtatagtt ttcttgtccg gatataaaaa
     5821 ccggaaattc ttcctgagca tccacagatg acatccaacc gaaaaagctg ataattaaaa
     5881 ggaggcaaaa tctcatatct aattgtatat catcgattat acagttttat cgactattat
     5941 aatcaaagtt ttttggcaag aacatttgcc tcaagttctg ctttatattt tgctattctg
     6001 tcatagattt catcattgga tgtacccagt atctgagctg ccagaatccc tgcattctta
     6061 gctccattaa gtgcaacagt agccaccggt attcctcccg gcatctgaag tatagaaaga
     6121 atggaatccc aaccgtcgat tgagttggaa gacttaatag gcacacctat taccggaagg
     6181 caactcaagc ttgctaccat gcccggaagg tgtgctgcac ctccggcacc ggcaattata
     6241 acacgaattc ctcgtgaata agcattttca gcgaattcaa acatcatttt aggtgtccga
     6301 tgggcagaaa caaccttctt ttcgtggggt ataccaagtt cttccaggat ttttgcagca
     6361 tccgacatca ccggccagtc actgtctgag cccataatta tacttgctaa aggcgtaacc
     6421 tcattcatga ttttctattt ttgaaatatt gcctgattgt caatagtttt tctttcagtt
     6481 tactcaagtc atctccgaca atattgatgt gtcccatttt gcgggcaggt tttatctctg
     6541 ttttcccgta taaatgaacg tacacttcat gcaggttcat gacttcatgc cacccggtcc
     6601 attcggcagt tccgtaatca tcctcttcac ctataatatt gtacatgata cctgcctttt
     6661 ccattacgct acttccgagg ggtaatccgg agattgctct caaatgctgc tcgaattgac
     6721 tgcaatgtgc attgttcatc gagatgtgca tgctgttatg aggtctgggc gcaatctcat
     6781 ttaaaaccac ttcaccgctt gaggtaataa aaaattcgaa agcacataac ccacatatat
     6841 tcaatgtttc tgttactttg actgcaatcc tgttcatttc ttccacgata tttttcccca
     6901 tgtttgcagg ataataaact tcggatagaa tgtgattttc aggatcaaaa tacatggcta
     6961 gaggcggata gagtacataa tttccatcgg gagaacgtgc aatcattacc gcaatttcaa
     7021 tatcaatgtc gatgcgttgt tcgaaagctc ccggtatatc atcaagggcc aggacatcgt
     7081 caattgctct aatggtgcgt actccttttc catcataacc catttcggct gatttgtata
     7141 tacaaggtaa gcgattcttc cattgttcaa atacaataac ggcttcatcc ttgctattaa
     7201 agctggaaaa ttctggtata ggaatgccaa ttgagtgata aaaggatttt tgtatgtttt
     7261 tattttttat tatctccagt gcatccggat ttgggtagac ctccttgccg gctttctcca
     7321 aatccctcaa tgcttcaagg ttgatatgtt cgatctcgac cgtaatcaca tctttatccg
     7381 ccccaaatga cataacatca tcataattgc ggatgtcacc tatagtatag aagtctctat
     7441 ataatgatgc aggagcattt tccgacttat ccatgcaata taccggcata tttcgttgct
     7501 gcgcttcagc ctgaagcatt ctccctaact gaccgccacc aaggatacct atttttttca
     7561 tcagagcggg atatgcaatt tattggttac tattaatgat aacatcaaaa tgatttctca
     7621 atatatttag gacggcatct gtattgccat tattattgat gatgatgtcc acatcatttt
     7681 tgtacgggaa tatatatttt tcgtaggaag gtaagacatg ggattcataa cgatatagta
     7741 catcttccaa tggataattt cgctcagact ggtcgcgttt gattcttctg atgattttca
     7801 aagcatcctt agcttcaatg tatatcttta aatcaagcaa tgatctcagg tccttcatat
     7861 gcatgataaa aaggccttca agaatcatga ccggtgccgg cttgatagta atcatctcca
     7921 catctgccag actgttattg aatgtgtatt cctcctgaac aatggtttct ccactcacca
     7981 ggcggatgat atcgttaaag aatgcctgtg tatcaatgga ttcaaccaaa tcaaaattct
     8041 gaactccgtt ttcatcatat acttgttgag tccttggttt gtaatagtta tccattgata
     8101 atatgcagac ttcttctgca ctatatcctt cagaaaggct cttaagaaat gttgtctttc
     8161 cagacccact acctccggtg attccgacaa tatatggctt gtacatcaaa tttgtcaatt
     8221 tatccttatg cgcaaaaata ggctttcact ccaagaaaat aggaattatt taagaggtaa
     8281 ataaaataaa ttatatttta gcgcttcatc aatcatgtcg aataaagagg tacgacaatt
     8341 ttgtcggatg attcattcta tatatgatat aaatttgagt aatttgatga tggataataa
     8401 actattattc gtatttgctc ataatacttt aaattattat tttttagatg gaaaaattaa
     8461 atattgccgg agaattaagt gccgataata tcaggatgct gatcagtggt atgcagaggc
     8521 gtttggagaa aatatacaag ggagggggtg agaaaaaaat tgaaaagata aaggccgaaa
     8581 atcgcttgcc agcacgggag cgcgtatcaa tgcttcttga tagtgaccga cccagtattg
     8641 aaattggcgc cttcgccggt tatgagatgt atcctgaata tggcggatgc ccagccggtg
     8701 gagtcataat ctttttaggt tatgtacatg ggagaatttg ccttgtagt
//
LOCUS       DAOQQN010000096         8589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3175_length_8589_cov_5.402859, whole genome shotgun sequence.
ACCESSION   DAOQQN010000096 DAOQQN010000000
VERSION     DAOQQN010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3175_length_8589_cov_5.402859"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLG24_05105"
     CDS             <1..348
                     /locus_tag="PLG24_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HMZ24300.1"
                     /translation="QEVEALCNRVIIINKGKIVLDDNTEKLQTVSKGSTTVMVQFVSP
                     GKIDTTRFKHIKGLKDIKHTENNVFEFTSERDSDIREQIFDSCVSAGYKIIRMEHRQF
                     SMEDIFQQLTLKS"
     gene            354..1085
                     /locus_tag="PLG24_05110"
     CDS             354..1085
                     /locus_tag="PLG24_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMZ24301.1"
                     /translation="MWTIAFKEASSFFSSLAGLIISGVFLLVMGLMLFFFPDTSILSG
                     NYANMDGLFYLAPTIFTFVIPAITMRFFAEELQTGTIELLATKPISDLKIIMGKFLAG
                     LFLIFLCLVPTLVYYYTVYQLGSPVGNLDTGGIMGSYIGLFFLSASFLSIGIFASALT
                     HNQVVAFVIGTFLCFICHWFFLYVSKIPAFVGNVDYILQKFGIDYHYSRMSKGVIDLK
                     DVVFFLSVISIFLSLTHFVLQRRKW"
     gene            1079..2821
                     /gene="gldG"
                     /locus_tag="PLG24_05115"
     CDS             1079..2821
                     /gene="gldG"
                     /locus_tag="PLG24_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HMZ24302.1"
                     /translation="MVKTNQSKNKAEKAEMSPRKQGYIILGLMFGFVFIANILASFTN
                     LNIDLTEEKRYTLTPATVKMLRGLQDNIYVEVLLEGKFPAGFKRLQESTQSVLDQFRN
                     QTSFVQYRFLDPLDTKSIEDKNERIKDLAEQGIKPTNLRVMENNESIEKLIYPVAVVK
                     YGSQIIPVNLLENSVAGFSDEEVLNQSINLLEYKLADAINKILRKNKPTIAITKGHGE
                     SKPVETRDLINTLAPFYDLKWIDLDSMYRIPDEVNLVIMPNPSKPYSEKAKFILDQYI
                     MRGGNTMFLINRLKIDMDSLRNPKSEYVPIENDVNLEDMFFKYGVRIEPNLVLDMECS
                     KIPLVVGKLGTGVQTELFKWYYAPLLSTRVQHPIVKNIDRVNAYWTSSIDTVKTKTHI
                     AKTNLLNSSQHSRLQYAPVRINFEILRYDPDPSKFNKPYQPVAWLLEGPFPSLYENRV
                     SPDMLEGLKQIKMEYLPVGKPAKMLVMSYGKLINNLYNPENNEYMPMGYNTFDKILYG
                     NKDFVINAVEYMFDESGILSARAKEVKLRLLDNVRIKEEKTYWQMLNVLLPISLLVLG
                     TFVFNYIRRKRFAS"
     gene            2840..3841
                     /locus_tag="PLG24_05120"
     CDS             2840..3841
                     /locus_tag="PLG24_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24303.1"
                     /translation="MSSKLKYIIGLFLSLLIVTGIVYFNKSGKSSMADSSRDFAIEDI
                     EDIGKIFVTDREGNHYSLDRKGNVWYADSVYLADPGFVDVVLTTLKRMTVVNLVPKPA
                     IKNVMRDIATLGKKVEIYDRKGRKLRSFYIGGTNQRVTASYMVLEGYNIPYEMSYPGF
                     SGDLGGRLWPLHLIDIRSKDIFRYKAGDIKKITVTYPRDKNESFTLTISNSNKYDIEP
                     LSQTVTPIAKPISKGAVEQYLSAFENIQAAKVVEKNLLNADTMVSSVPFATFEIVDRN
                     DNIRKLDLYPIMDESARVGLGSEPEFFFAILDNKNFFNCQLIVMKKLFWGYSFFFDR"
     gene            3838..5070
                     /locus_tag="PLG24_05125"
     CDS             3838..5070
                     /locus_tag="PLG24_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HMZ24304.1"
                     /translation="MRKLIILLVLSILCFGFITEKNNYEDWGFFGHRRINRYAVFTLP
                     QEMLPLFKGNIDFLTEHAVDPDKRRYAFRLEAPRHFLDMDRYKDACLPENFVEAFSAF
                     AMLKVITHTNDTLLIHSSDTSWCNNLPNAVMTDGSNVWNIGKKELRHHFRQFILPAYY
                     DLRYQINPDSIRPLLPTHIQAIKNISLIDSFTIHGIVPYIIEQQYDKLVRAFKKLDKN
                     EIINIAADLGHYIADAHVPLHACSNYNGQKTDQYGIHAFWESRIPELFADTQYDFLVG
                     KATYIRDVRKFAWETIRTSGSLADSVLSMEAALKSFYPRDQQMCFDQRLGQTVWIQCR
                     DYAAAFQDKMQGMVEIRMREAIHSVGSLWYSAWVEAGSPPLNFSNDIKAAWKIDSTVL
                     EYDNIIKKGGDGYGRNHE"
     gene            5231..6658
                     /gene="miaB"
                     /locus_tag="PLG24_05130"
     CDS             5231..6658
                     /gene="miaB"
                     /locus_tag="PLG24_05130"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HMZ24305.1"
                     /translation="MRTQELLDRPHSVDVPEEMQGSVYLQHGITYGSKKFYIESYGCQ
                     MNFSDSEIVASILSKEGYGSTADIYEADLVLINTCSIREKAEDTIRKRLEYYRQIKKT
                     NKSMMVGVLGCMAERMKKTLLEEEKLVDIVVGPDAYRDLPNLIAGASEGERQINVLLS
                     REETYADISPVRLDSNGVSAFISIMRGCNNMCSFCVVPFTRGRERNRDPYSIVSEARD
                     LFENGYREVTLLGQNVDSFNWKSMDGNEQVNFAQLLEMVAQVDPLLRIRFSTSHPKDI
                     TDDVLYTMAKYENICKYIHLPVQSGSSRILTLMNRTYDADWYRSKIDRIYEIMPECAI
                     SSDIIAGFCSETDDEHRESIAMIDYARYTMSYMFYYSERPGTPAAKKLVDDVPMEIKI
                     SRLNEIIEKQRQISAEANKSDEGKEFKILIDGYSKKSESQMKGRTSQNKVVVFDIHPD
                     YKIGDYAKVYITGSTTATLFGEIIH"
     gene            6727..8010
                     /locus_tag="PLG24_05135"
     CDS             6727..8010
                     /locus_tag="PLG24_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HMZ24306.1"
                     /translation="MEALQNIKRKFGILGVSATLDNALNTAIRVAPTDLTVLISGESG
                     VGKDVFSRVIHSLSPRKHNPFIAINCGAIPEGTINSELFGHEKGSFTGALGDRKGYFE
                     TVHGGTIFLDEIGEMPLDTQAFLLRVLESGEFIRVGSSKTLKTDVRVIAATNVNLEEL
                     IRKGKFREDLYFRLSIVPIKVPALKERREDIKILFRKFALDFSEKYNTNSIQLNDHSL
                     SILENYSWPGNIRELRNITEQLSVLSEERIITPEILLRIAPQLANRNLPSIIKGQEDD
                     HFTERDILYKLLFEMKGDMNDLKTLVFELITRNQLNVGDLSNLRQLKHPTSQQDFSAF
                     SGFNNNQANQPAHTILEPSVPAAHHTYLPDEPDEEVYQHAEEVEDVLSLDAMEKDLIQ
                     KALKKYNGRRKEAAKELGLSERTLYRKIKQYDING"
     gene            8093..8401
                     /locus_tag="PLG24_05140"
     CDS             8093..8401
                     /locus_tag="PLG24_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HMZ24307.1"
                     /translation="MFGNFEEQQKEMEKKLKAITVHKASSENEINITMNASLEVENIS
                     IDLSKLDLTSSDQLEDLILVTMNEAIQQAQLAQAAESQKMLANMMPGGLGDLGKLFGQ
                     "
BASE COUNT         2739 a         1655 c         1737 g         2458 t
ORIGIN      
        1 caggaggtgg aagctttatg caaccgcgta attattatca ataaaggtaa gatcgtcctg
       61 gatgataata ccgaaaaact tcaaacagta agcaaaggca gcacaacagt gatggttcaa
      121 tttgtttctc ccggaaaaat tgatacaaca aggtttaagc atatcaaggg attaaaagac
      181 atcaaacata cagaaaataa cgtatttgaa tttacatcag aaagggattc agatatcagg
      241 gaacaaatat ttgatagttg tgtctctgcc ggttacaaaa taattcgaat ggaacatcgc
      301 caattcagta tggaagatat tttccagcaa ttaacgctca aatcctgaca tttatgtgga
      361 ccattgcttt taaagaagct tcttcatttt ttagctcgct ggccggttta atcatcagtg
      421 gtgtatttct gctggtcatg ggactgatgt tattcttttt cccggatacc agcatactta
      481 gtggaaatta tgccaatatg gatggtctgt tctatttggc ccctactatt tttacctttg
      541 tgattccggc aatcactatg agattttttg ctgaagaact tcagacagga accatcgaac
      601 tgttagctac caaacccatt tctgatttga agatcattat ggggaaattt ttagcaggct
      661 tgtttctgat cttcttatgc cttgtaccga cccttgtcta ttattacaca gtttatcaat
      721 tgggttctcc tgtaggtaat cttgatacag gcggaattat gggctcttat attggccttt
      781 tctttctttc ggccagtttt ctttctatcg gcatttttgc cagtgcactg acacataatc
      841 aggtagttgc ttttgtaata ggcacattcc tttgcttcat ttgccactgg tttttcctgt
      901 atgtcagcaa gattcctgcc tttgtaggaa atgttgatta tattttgcaa aaattcggaa
      961 tagactacca ttattcccga atgagcaagg gagttattga cctcaaggac gtggtgttct
     1021 tcctttcagt aatctccatc tttttgtcat tgactcattt tgtattgcaa cgaagaaaat
     1081 ggtaaaaaca aatcaaagta aaaataaggc cgaaaaagct gaaatgtcgc ccagaaaaca
     1141 aggctatatc atccttggtc tgatgtttgg cttcgttttc attgctaata tacttgcttc
     1201 atttaccaat ctgaatattg acctgactga ggaaaaaaga tacaccctga cccccgctac
     1261 ggtgaaaatg cttcgcggac ttcaagacaa tatttatgta gaagttttac tggaaggaaa
     1321 atttccggcc ggctttaaac gccttcagga atccacccaa tcagtattgg accagttccg
     1381 caatcagaca tcctttgtac agtatcgttt tcttgatccg cttgacacaa aatcaataga
     1441 agataaaaat gaacgtatca aagatctggc agagcaagga ataaaaccta ctaacctacg
     1501 ggtcatggaa aataatgaat ctattgaaaa gttgatttat ccggtggcag tagtcaaata
     1561 tggttcccaa atcatccccg tcaaccttct ggaaaattca gtcgccggtt tcagtgatga
     1621 agaggtgctg aaccaatcca tcaacttgtt ggaatacaaa ctggcagatg caataaataa
     1681 aattttaaga aagaacaaac ctacaatagc tataacaaaa ggtcatggtg aatcaaaacc
     1741 agtagaaacc agagacctta tcaatactct tgcccctttc tatgatctga aatggatcga
     1801 cctggatagt atgtacagaa tcccggatga agtcaatctg gtcatcatgc ctaatccttc
     1861 aaagccatat agtgagaaag ctaagtttat tcttgatcaa tatattatgc gaggcggcaa
     1921 taccatgttt ttgatcaaca gattgaaaat cgacatggat tctctcagaa accccaaatc
     1981 cgagtatgtc ccgatagaaa atgatgtcaa ccttgaggat atgttcttta aatatggagt
     2041 gcgtattgaa cccaatcttg tcctcgatat ggaatgttca aagattccgc ttgtagtcgg
     2101 aaaactgggg acaggtgttc agacggaact cttcaaatgg tattatgcgc cattgctgag
     2161 taccagagtt cagcatccaa tcgttaaaaa catcgacaga gtcaatgcat actggacatc
     2221 cagtatagat acagttaaga caaaaaccca tattgctaaa accaatttgc ttaactccag
     2281 tcagcattca aggttgcaat atgcgcctgt ccgaattaat tttgaaatcc tgagatatga
     2341 ccccgatcct tccaagttca ataagcccta ccaaccggta gcctggcttc ttgaagggcc
     2401 atttccctcc ttatacgaaa atcgggtcag tccggatatg ttagagggat taaaacaaat
     2461 caaaatggaa tatcttccgg ttggcaagcc agccaaaatg ctggttatgt catacggcaa
     2521 gcttatcaat aacctctaca accctgaaaa taatgaatat atgccgatgg gttataacac
     2581 atttgataaa atattatatg gcaacaaaga ttttgtaata aatgccgtgg aatatatgtt
     2641 tgatgaatca ggaatccttt ctgccagggc aaaggaagtg aaattgcgtc tattggataa
     2701 tgtcagaatc aaggaggaaa agacctattg gcaaatgctg aatgttttgt taccaatatc
     2761 actccttgta cttggaacct ttgtattcaa ttatatcagg aggaagagat tcgcttcata
     2821 ataattcaga accattgata tgtcttctaa attaaaatac atcataggcc tttttctatc
     2881 actattgatc gttacaggca tcgtttactt caacaagtcc ggcaaatcct ccatggctga
     2941 cagtagccgt gattttgcaa tcgaggatat agaagatatc ggtaaaatat ttgttactga
     3001 tagggaaggc aaccattatt ccctggatag aaaaggtaat gtttggtatg cggacagcgt
     3061 atatctggct gatcccggat ttgtggatgt ggttcttact actttgaaaa gaatgacagt
     3121 ggttaatctt gtcccaaaac ctgccatcaa aaatgtcatg cgtgatatag ctaccttagg
     3181 caaaaaagtg gaaatatatg accggaaagg acgaaaacta agatctttct atataggtgg
     3241 gacaaatcaa agagtcaccg cctcctatat ggtgctcgaa ggttataata ttccctatga
     3301 aatgagttat ccgggattca gcggagacct cggcggtaga ttatggccac tacatttgat
     3361 tgatattaga tcaaaagaca tttttagata taaagccgga gatataaaga aaataaccgt
     3421 aacatatccc agggacaaaa atgaatcttt caccctgacc atttcaaatt ccaacaaata
     3481 tgatattgaa ccgctcagtc aaacagttac ccccatcgca aagcctatca gtaaaggtgc
     3541 agtcgaacaa tatctcagtg cgtttgaaaa tattcaggct gcaaaagtcg ttgaaaaaaa
     3601 cttactaaat gctgatacaa tggtatcaag cgtccctttt gctacttttg aaatagtgga
     3661 tagaaatgat aatatcagga aactcgatct ttatcctatc atggatgagt ctgcacgtgt
     3721 cggacttggt tcagaaccgg aattcttctt tgcaatattg gataataaaa acttcttcaa
     3781 ctgtcaatta atcgtgatga aaaagttatt ctggggctat tctttcttct ttgacagatg
     3841 agaaaattaa taattcttct ggttttaagc attctgtgct ttggctttat aacagaaaaa
     3901 aataattacg aggactgggg tttctttggg catcgcagaa tcaatagata cgctgttttt
     3961 acattaccac aagagatgtt acctcttttt aaaggtaata ttgattttct tacagaacac
     4021 gccgttgatc ctgacaagag acggtatgct tttcgattgg aagcacctcg acattttctg
     4081 gatatggaca ggtacaaaga tgcctgtctt ccggaaaatt ttgtcgaagc cttctctgca
     4141 tttgcaatgt tgaaggtcat tacccatact aacgatacct tacttatcca ctcatcagat
     4201 acaagctggt gcaacaacct cccaaatgcc gtcatgacag atggcagcaa tgtatggaac
     4261 atcggaaaaa aagagttgag gcatcacttt cgtcaattta tattacctgc ctattatgac
     4321 ctccgttatc aaatcaatcc ggattcaatc agacctttat tacccactca tattcaagct
     4381 attaaaaaca tttccctaat tgatagcttc acgatccatg gcatcgttcc ttatattatt
     4441 gaacaacagt atgataaatt agtacgtgca ttcaagaaat tggataaaaa tgaaatcatc
     4501 aacattgcag ccgatttagg acactatatt gctgatgcgc acgtacctct gcatgcctgt
     4561 agcaattata acggtcagaa aacagaccaa tacggtattc atgcattttg ggaaagccgc
     4621 attccggaat tatttgctga tacacaatat gatttcctgg tgggcaaggc gacttatatc
     4681 agggatgtcc gcaagtttgc ctgggaaacc atcaggacaa gcgggtcact tgccgacagt
     4741 gtgttatcaa tggaagccgc actgaaatca ttctatcccc gggatcagca aatgtgcttt
     4801 gaccaacgat taggacaaac agtgtggatt cagtgtcgtg attatgcagc cgcttttcag
     4861 gataaaatgc agggtatggt tgaaatccga atgagagaag ctattcattc cgttggttcg
     4921 ctctggtatt cagcctgggt agaggcaggg tcaccacctt tgaatttttc aaatgacatc
     4981 aaagctgcct ggaaaatcga ttccacagtt ttagaatatg ataatatcat caaaaaaggt
     5041 ggagacgggt acggtcggaa ccatgaataa taagccaaag ccaaaatagg cttgattggt
     5101 ttaaccgaca tccaaagtta gaaccacaac tttccaaccc tgttattcat ttctggacca
     5161 taagtacttt tgtcatatca tgacgaaatg tcagattttc ttaatacctt tgtgccttta
     5221 attttgaaac atgcgtacac aagaattact tgacagacca cactccgttg atgtgccgga
     5281 ggaaatgcag ggttcagtat acctacaaca tgggattaca tatggatcca aaaagtttta
     5341 tatcgaaagc tatggttgtc aaatgaattt ttctgactct gaaatagtag ccagtattct
     5401 ctcaaaggaa ggatatggat cgacagcaga tatttatgaa gcagatctgg tattgattaa
     5461 tacctgctct ataagggaaa aggccgagga cactatacgc aaacgccttg aatattatag
     5521 acaaatcaag aaaaccaata aatccatgat ggttggtgtc cttgggtgta tggctgaaag
     5581 gatgaagaaa accctgctgg aagaggaaaa actggtagac atcgttgtcg gccctgatgc
     5641 ttacagggat ctgccaaatc taatcgcagg cgcatcagaa ggcgagcgcc aaatcaatgt
     5701 tttactttcc cgtgaggaaa cctatgccga tataagtccg gtacgactgg attccaacgg
     5761 agtttctgca tttatcagca tcatgcgggg ttgtaacaac atgtgttcgt tttgtgtagt
     5821 cccattcacc agagggcggg aaagaaaccg ggatccatat tcgatcgtat cagaagcacg
     5881 ggatttgttt gaaaacggtt atcgtgaagt gactctactc ggtcaaaatg tcgattcttt
     5941 caactggaaa agcatggatg gtaacgagca ggtcaatttt gcgcaattat tggaaatggt
     6001 cgcgcaggtt gatcctttac ttaggattcg gttctcgaca tcacatccca aagacatcac
     6061 ggatgatgta ctctatacta tggcaaaata tgaaaatatc tgtaagtata ttcacctgcc
     6121 tgttcaaagc ggttccagtc gtatcctcac attaatgaac cgtacctatg atgcagactg
     6181 gtacaggtct aagatagaca gaatatatga aatcatgccc gaatgtgcaa tttcaagcga
     6241 tatcatagca ggattctgtt cagaaaccga cgatgaacac agagaaagca tcgcgatgat
     6301 agattatgcc cgatatacga tgagttacat gttctattac agtgaaagac cgggaacccc
     6361 tgcggctaaa aaactggttg atgatgtacc aatggaaata aaaataagtc ggttgaatga
     6421 aataatcgaa aagcaaagac agataagtgc tgaggctaac aaatcggatg aaggtaagga
     6481 atttaaaatc ctcatcgatg gatactctaa aaagtccgag tcccagatga aaggacggac
     6541 cagccaaaat aaagtggttg ttttcgatat tcacccggat tataagatag gtgactatgc
     6601 caaagtatac attaccggta gtacgacagc gactctattt ggcgaaataa ttcactgaaa
     6661 ataaatattt tataccaaaa ttgacttgta aacgcttcat ttgaattttt taataaagat
     6721 aaatccatgg aggcactgca aaacataaaa cgaaagtttg gcattcttgg cgtttccgcc
     6781 acattggaca acgcattgaa cacagccata cgtgtagcgc ctacagatct gactgttctg
     6841 atatcaggag agagcggggt aggtaaggat gttttttcca gggtaattca ttcattaagt
     6901 ccaaggaagc ataatccctt tattgccatc aactgtggtg ccattcccga aggaactatc
     6961 aatagtgaat tatttggcca tgagaaaggc tcatttacag gtgctttggg tgacagaaaa
     7021 ggatattttg aaacagtcca tggtggaacg atatttctgg acgaaatagg agagatgcca
     7081 cttgatacgc aggcctttct tctgcgagtc ctcgaatccg gtgaattcat cagggttggt
     7141 tcgtccaaaa ccctaaaaac tgacgtcagg gtaattgctg ccaccaacgt caatctggaa
     7201 gaattgatca gaaaagggaa gtttagagaa gacctttatt tcagactttc cattgtgccc
     7261 ataaaggtgc cggcgttgaa ggaaagacgt gaagatatca agatcctctt cagaaagttt
     7321 gcgcttgatt tttctgaaaa atataatacc aacagcattc aacttaatga tcacagcctt
     7381 agcattcttg agaattattc ctggccgggc aacattcgtg aattgcgaaa tattacagaa
     7441 caactttcgg ttctttccga agaaagaatc ataacgcctg aaattctcct tcggattgct
     7501 cctcaactag ccaatcgcaa tcttccttca attattaagg gacaggaaga tgatcacttt
     7561 acggagaggg atattctata caaattgctt tttgagatga aaggcgatat gaatgatttg
     7621 aaaacattgg tctttgaatt aattacaaga aatcaactga atgtgggcga tctttccaac
     7681 ctcaggcaat taaaacatcc aaccagccaa caggactttt cagcattttc aggattcaat
     7741 aacaaccagg ccaatcaacc tgcacacacc attttagaac caagtgttcc tgcggctcac
     7801 catacgtact taccagatga gcctgatgaa gaggtgtacc aacatgcgga agaagtggag
     7861 gatgttttat ctcttgacgc tatggaaaag gatctgatac agaaggctct taaaaaatat
     7921 aatggtagac ggaaagaagc tgccaaggag ttaggccttt cggaacgaac actatatcga
     7981 aagattaaac aatatgatat caacggatga ttgtattgtg cgccagaagg aaatcttaat
     8041 ctttattcaa tattattgac ttattttcgt ttaaaatata aatttattta tcgtgtttgg
     8101 caattttgag gaacaacaaa aggaaatgga gaagaagctt aaggctatta ctgtacataa
     8161 ggcttcttcg gaaaacgaaa tcaacatcac tatgaatgca agcctggagg tggaaaatat
     8221 ttccatagat ctaagcaaac ttgacctaac ttcatcagat caactcgagg atcttattct
     8281 ggtaacaatg aatgaagcca ttcaacaggc tcaattagca caggctgccg aaagtcaaaa
     8341 aatgttggct aatatgatgc cgggaggatt gggtgatctg ggaaaactat ttggtcaata
     8401 aatcacagtc tttgttaaat cctgacaatg ttaagacata tatttctctt attacttacg
     8461 tgtagttttt ttgctccgat cgtcaatgca caaataactg ataatcttta cgctttttac
     8521 aattttgacg attgctcaac cagggatgta cagggtagca accatggagt gccacatgta
     8581 actccggat
//
LOCUS       DAOQQN010000097         8554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3191_length_8554_cov_5.352865, whole genome shotgun sequence.
ACCESSION   DAOQQN010000097 DAOQQN010000000
VERSION     DAOQQN010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8554
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3191_length_8554_cov_5.352865"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PLG24_05145"
     CDS             <1..613
                     /locus_tag="PLG24_05145"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ24308.1"
                     /translation="RMPTEVQDILITVLSEKMMSIPELNMEVYAQKGFNVIATANDRD
                     KGVHDLSAALRRRFNIVYLSMPETLQEEIDIIHFRLGQIQRDLNLEKYKVPLQHLSDL
                     ITIFRELRNGETEEKKSKIKSPGSSLSTAEVLSVLQTGIQHSIHFGTGNLEPSDVASS
                     IISTVIKDPYQDKAAWDEYLESVIKNRKGWKPWYEAFSSASES"
     gene            complement(608..2911)
                     /locus_tag="PLG24_05150"
     CDS             complement(608..2911)
                     /locus_tag="PLG24_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ24309.1"
                     /translation="MNFSPEQGSLLAYEHYGITATARPLNGYDEQNFLLTAQDGNKYT
                     FKIAGSDISRTFLEAQADVMHHLDHSSIQGGFPVCLENKQGKAITTITYEGKEYHIRI
                     LTYLEGHFWVDEKNKNNFLYESLGEFLGAMDRSLEGICPDATVRNYVWDNNRAPEAEK
                     KLHFIRDHEKRRIAAYFLLQFRTEVTPVLSELRHACIHGDANDYNLLVRNGRVSGLID
                     FGDMIYSATINNLAIACAYALLHTEDPIEKASLIVKGYHSQYPLTEQEIDILYYLIAG
                     RLCISVTQSAEKSYSNSENEHHFLSEQSAWRLLYQLIRTNPLYAADQFRRSCGMMPCL
                     NPEDDYSSLLSERNRYIGRNLSISYRTPLKIVKGALQYLYDDKGKTYLDCVNNVSHVG
                     HCHPGVVYAIQSQAAILNTNTRYLHDNITSYAKALVDTLPERLSVCYFVNSGSEANDL
                     AIRMARHFTGHNDIVVLDHAYHGTSTLAMEISPYKFDGIGGFGARHYIHKAASPNLYR
                     GPYGIDDEAAGEKYAQSVKEIVQGLEREDKSPAAFICETLLGVGGQIPLPTGYLKQVY
                     QYIRSAGGVCIADEVQVGFGRIGEAFWGFELQEVTPDIVVLGKPIGNGHPLAAVVVTQ
                     AIADAFNNGMEYFNTFGGNPVSMAAGMAVLNIIRQEELQQHALNTGNGLMDDLRKLME
                     KHTIIGDVRGHGLFVGVELVRNRVSKEPAVPEIDVIVERMKGRGFLLSTDGPLHNVLK
                     IKPPMVISRQNCVDLVQALDEVLSEEL"
     gene            complement(3022..4383)
                     /locus_tag="PLG24_05155"
     CDS             complement(3022..4383)
                     /locus_tag="PLG24_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24310.1"
                     /translation="MKQQYTSTNFIRKNTRSLPIGNCYINKTWEVNKLANVIVSRKLK
                     NGNLIVGFYLVDLFCLGLRSSYFQSDMEEESLKNWLKKVSYLESKFIQIDYVLAHNII
                     YGSYEYGLENGIPQHKDFDKVTKYILEEDDERIPLIELEFGRDGKPFYFQTESQSDAF
                     AENIIRLLKKNVGEGNFYYTLLKDIEEESEEEDEEVDDEEDPNNFNLPILKYLLNDGT
                     VDIDKAKHTFNTLLDDGEMFAQYPLFISASMAIIHHYADEQRTNQLFEKWELDTTIDP
                     EYEIEAGMSEQQIDRTYEILTEGKPSLKDIRYIRKNISSEVLADYILMKASLPKIDHE
                     NEDDSDLDYSEYNKREEQFLKDHPDYPLSKALIASETSSPDDLTIESIFGSADNISVF
                     EFNEYLMMKLETLKNKPDASAFLAFRAILGQYLESDTVHEEIFFTYELLTIQYIGDYL
                     MDN"
     gene            complement(4456..5778)
                     /locus_tag="PLG24_05160"
     CDS             complement(4456..5778)
                     /locus_tag="PLG24_05160"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMZ24311.1"
                     /translation="MLFNEMKIPKPILRALDDMGIAEASAIQELVFSAVKSGRDIGGI
                     AQTGTGKTLAYLVPVLSLHQYSTAKHPSILILVPTRELVKQVEDAIVQLTGYMSVRTY
                     GIFGGVNTKPQADRILEGLDILVGTPGRVVDFVSTGILNLKNIKRLIIDEFDEMLNQG
                     FRAQLKVIFEKLPEKRQNLLFSATLNPEVEEMMDEYFERVENIEATPIGTPLETIEQQ
                     KYHVPNFYSKLSLLKTMLPDQNIRKAIVFVSNKKKAELLFDLLQQTGIDDIAYMHANK
                     HQKRRFDTLDAFESGDSRILIATDLLSRGLDIKDVTHVINFDLPELAENYIHRIGRTG
                     RFGKKGTAISFIEQKDLGLLEALEGMMGTELGERALPADYEQSEELLPEEKERIYMPD
                     YLGQSFTKSDGGAFHEKLKKNKKVNIRFDYEASMKKKYGKQYRPKHAR"
     gene            complement(5896..7008)
                     /locus_tag="PLG24_05165"
     CDS             complement(5896..7008)
                     /locus_tag="PLG24_05165"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HMZ24312.1"
                     /translation="MNIIFIKKYASLLLNYCMELKSGERLLVSTTTLAEPLVAELYRQ
                     CQQMGVIMEAILEWEGKSDDFNDYGHNAQAGYINPLYKEAMESFEAYLVIRAPFPSKS
                     RSKPNSTLNEVRAAANAQFHKLYFERTATRNLRRNLCQYPTQAGADLAGMSLEEYTSF
                     VANACFLNTDNPEQEWLNVRARQQSITDLLNSCKEFRYLSPNMDISFRTEGRRWINSD
                     GQTNMPSGEIYTSPEENSVNGHILFTLPSLYQGHLLNNVRLEVRDGYITSWTCDNDID
                     ILNEVFRIEGTRRFGEAAIGTNYRIDRLTKNILYDEKIGGTVHMAIGQSYLQAGGKNE
                     SSVHWDMISDMKDGGKIYADGQLVYENGKFLEVILE"
     gene            7127..7426
                     /locus_tag="PLG24_05170"
     CDS             7127..7426
                     /locus_tag="PLG24_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24313.1"
                     /translation="MKAILTILIMFFAFHLCSAQDIIQAQRDQEAKIKKIYNQKKITE
                     LEYSKLMKDQNNIKDAIQIAKADGVMTPKEKNAINKKLMHVEKKIIKYQRNSEIY"
     gene            complement(8282..8443)
                     /locus_tag="PLG24_05175"
     CDS             complement(8282..8443)
                     /locus_tag="PLG24_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24314.1"
                     /translation="MRNERNNTEFRRRFNQISIEWEIALEQGRATNLEFHGEVVLVQV
                     HHQRYYSKK"
BASE COUNT         2310 a         1778 c         1780 g         2686 t
ORIGIN      
        1 acggatgcca actgaggtgc aggacatcct gattactgtt ctttctgaaa aaatgatgag
       61 cattcccgaa ttaaatatgg aagtatatgc acaaaaagga ttcaatgtga ttgcaaccgc
      121 caacgaccgg gataaggggg tgcacgatct ttctgccgct ttgcggcgtc gttttaatat
      181 cgtgtatctg tcaatgccgg agacccttca ggaagaaata gatatcattc atttccgatt
      241 gggtcagatc cagcgggacc tgaatttgga gaagtacaag gtgccgttgc agcatctttc
      301 agatttgatt accattttca gggaattaag gaatggagag acagaagaaa agaaaagcaa
      361 gatcaaatca ccaggcagtt cattatccac tgctgaagtg ttatccgtgc tacaaacagg
      421 tattcagcat tccatccact ttgggacagg caacttagaa ccttccgatg ttgcatcatc
      481 catcatcagt acggtcatta aggatcctta tcaggacaaa gccgcctggg atgaatacct
      541 cgagtctgtc atcaaaaaca ggaaaggttg gaagccttgg tatgaagctt tcagctcagc
      601 atcagaatca taattcctcg gacaaaacct catccagtgc ctgcaccaaa tcgacacagt
      661 tctgacggct gatgaccatt ggtggcttaa tcttgagtac gttgtgcaaa ggcccgtcag
      721 tactgagcag gaagccacgt cccttcattc tttccacaat gacgtctatt tccggcacag
      781 cgggctcttt gcttactcga ttccttacca gctcaacacc gacaaacagt ccatgaccac
      841 gtacatcacc gatgatggta tgtttttcca tcagtttcct caagtcatcc attagtccat
      901 ttccggtatt gagagcatgc tgttgaagtt cttcttgccg gatgatgttg agcacagcca
      961 taccagccgc catagagacc ggattgccac cgaatgtatt gaaatattcc ataccattgt
     1021 tgaaggcatc tgcgatagcc tgagttacca cgacagctgc caatggatgg ccgtttccga
     1081 tgggcttgcc caagactacg atatcgggtg tgacttcctg aagctcaaag ccccagaatg
     1141 cttcaccgat cctgccgaaa ccgacttgca cttcgtctgc aatgcataca cctcctgctg
     1201 agcggatgta ttgatatacc tgtttcagat atcctgttgg caatggtatc tgaccgccca
     1261 caccgagaag tgtctcacag ataaatgcgg cgggggattt atcttcccgt tcaaggcctt
     1321 gtacaatttc cttgacgctt tgcgcatatt tttctcctgc tgcctcatcg tcaatgccat
     1381 aggggcctcg gtatagatta ggtgaagctg ctttgtggat ataatgcctg gctccaaacc
     1441 ctccaatacc atcgaatttg tacgggctga tttccatggc taacgtggag gtgccatggt
     1501 aggcatggtc gaggacgaca atatcattgt gtccggtaaa atgtcttgcc attcgtatcg
     1561 ccagatcatt agcttcactg cccgaattga caaagtagca aaccgataag cgctcaggaa
     1621 gtgtgtcaac aagggctttg gcgtagctgg taatattgtc gtgcaggtag cgcgtgttgg
     1681 tattgagtat agcggcctga ctttggatag cataaacgac acccggatga caatggccga
     1741 catggctcac attgttgaca cagtcaagat aagttttgcc tttgtcgtcg taaagatatt
     1801 gcaaggcgcc cttgacaatt ttcagtggtg tacgatagct gatgctcagg ttgcgtccta
     1861 tgtaccgatt cctttcagac aacagtgagc tgtaatcgtc ttccggattc agacagggca
     1921 tcattccgca tgaccttctg aattgatcgg ctgcatagag tggattggtg cggattagtt
     1981 ggtacaacaa tctccatgcc gattgctcag acaggaagtg atgctcattt tcgctgttgc
     2041 tataagactt ttcggcagat tgagtgacgc tgatacagag tcgtccggca atcaggtagt
     2101 ataatatatc gatctcttgc tcggtaagag ggtattggct gtgatagcct ttgacgatca
     2161 ggcttgcctt ttcgatcggg tcctcagtat gtaacagggc ataggcacaa gctatggcga
     2221 gattgttgat cgtggcagaa tatatcatat caccaaaatc tataagcccg gagactcttc
     2281 cattgcggac cagcaggttg taatcattgg catcaccatg tatacatgca tgcctcagtt
     2341 ccgataatac aggtgtcact tctgtcctga actgtaacag gaaatacgct gcaatccggc
     2401 gtttttcatg atcgcggatg aagtgtagtt tcttttcagc ttccggagcc cggttattgt
     2461 cccaaacata atttcttact gtcgcatccg ggcaaatgcc ctccaacgac cgatccatag
     2521 cacccaggaa ttctcccagg ctctcatata ggaagttgtt tttgtttttc tcatctaccc
     2581 aaaaatggcc ttccaaatag gtaagtattc tgatatgata ttctttacct tcataagtta
     2641 tggtagtgat ggcttttcct tgcttgtttt caagacatac cggaaatccg ccttgaatgg
     2701 aagagtgatc cagatgatgc atcacatcgg cctgtgcctc caggaaagtt cggctgatgt
     2761 ctgaacctgc aatcttgaaa gtatatttat tgccgtcctg tgcagttaat aaaaagttct
     2821 gttcgtcata gccattgagt ggtctggcag tagcagtaat gccataatgc tcataagcca
     2881 acaaacttcc ttgttccgga gaaaaattca tttttgaata ttattgttca aagataaaat
     2941 tttgtgtttt acccactttt gcagccggta tttctaaaag caaaattgta agaaaatgcc
     3001 aatgaaaaat aaaacggcaa attaattgtc catcaaatag tcacctatat actgaatggt
     3061 caataactca taggtaaaaa atatttcctc atgtactgta tcagattcca gatactgacc
     3121 caggatggct ctgaatgcga gaaacgctga tgcatccggt ttgttcttta atgtttccaa
     3181 cttcatcatc agatattcat tgaactcaaa aacggaaata ttatcagcac tgccaaatat
     3241 tgattcaatg gtaagatcgt caggcgagga tgtttcactt gcgatcaacg ctttacttag
     3301 tggataatca ggatggtctt ttaaaaactg ctcttctctc ttgttatatt cggaataatc
     3361 taaatcagaa tcatcttcgt tttcatgatc aattttaggc aaggaagcct tcatcagtat
     3421 ataatcggcc aatacctcac tacttatatt cttcctgata tagcggatat ctttcaaaga
     3481 tggcttgcct tcggttagaa tttcgtatgt tctgtcaatt tgctgctctg acattcctgc
     3541 ctcgatttca tattccggat caatagtcgt atcaagttcc cacttttcaa aaagttggtt
     3601 ggtccgttgt tcgtccgcgt agtgatggat gattgccata gaagctgaaa taaacagggg
     3661 atattgagca aacatttcac catcatccag taaagtatta aatgtgtgct ttgctttatc
     3721 aatatctaca gtaccatcat tcagtaaata tttcaagatc ggcaagttga agttgtttgg
     3781 gtcttcttca tcatcaacct cttcatcttc ctcttccgac tcctcttcta tatcttttag
     3841 taaagtataa tagaaattgc cctctccgac attttttttc aataaccgga taatattttc
     3901 tgcaaatgcg tcactttgag attctgtctg aaagtaaaat ggttttccgt cccggccaaa
     3961 ttccagttct atcaaaggaa tgcgttcatc gtcttcctcc aggatatatt ttgtcacttt
     4021 atcaaaatcc ttgtgctgcg gtattccgtt ttcgagtcca tactcatagc ttccgtagat
     4081 tatgttatga gccaggacat aatctatttg aatgaatttt gattccagat atgagacttt
     4141 tttcaaccag ttttttaaac tttcttcttc catgtcggac tgaaaatagc tgcttctaag
     4201 gccaaggcaa aatagatcga caagataaaa gcctacaatg agatttccgt ttttgagctt
     4261 tcgggaaacg atgacattgg ctagtttgtt aacttcccag gttttattga tataacaatt
     4321 accgattggg agacttcttg tatttttccg gatgaaattg gtagatgtat attgttgttt
     4381 catttgctta acatttgaaa gacaaggtgg gaagtttttc ttgcattctc ataaaaaacc
     4441 caataatttt ttttcttatc tggcatgttt aggacgatac tgctttccgt attttttctt
     4501 catagaagct tcatagtcga atctaatatt tactttttta ttttttttca gtttttcatg
     4561 aaatgcccct ccatctgatt ttgtaaatga ctgccctaag taatccggca tatagatgcg
     4621 ctctttttct tcagggagta attcttcaga ttgttcatag tccgcaggta atgccctttc
     4681 gcccagttca gtacccatca tgccttccaa tgcttctagc aatccaagat ccttttgttc
     4741 gatgaaggaa atggccgttc ccttttttcc aaaacgcccg gtgcgcccga tacggtggat
     4801 ataattttct gccagttccg gaagatcgaa gttgatgaca tgtgttacat cttttatatc
     4861 aagcccacgg gagagcaagt cggtagcaat cagaattctt gaatcaccgg attcaaaagc
     4921 atccagcgta tcaaatctcc tcttctgatg cttgttggcg tgcatatagg cgatgtcatc
     4981 aatgcctgtc tgttgcaata agtcgaaaag taattctgct tttttcttat tggaaacgaa
     5041 tacgattgcc ttgcggatat tttgatccgg caacatggtt ttcaacaggc tcaatttgct
     5101 gtaaaaattg gggacatgat atttttgttg ttctatggtt tccaatggtg ttcctatagg
     5161 agtagcttcg atattttcga ctctttcaaa gtattcatcc atcatctcct ccacttcagg
     5221 attgagggtg gctgaaaaca ggaggttttg tcttttttca ggcagctttt cgaagatgac
     5281 cttcaattga gcccggaagc cttgattgag catttcgtca aattcgtcga tgataagtct
     5341 tttgatattc tttaaattca gaatgccggt tgaaacaaaa tcgaccaccc tgcccggggt
     5401 gcctaccagt atatcgaggc cttccaggat tctgtctgcc tgaggcttgg tatttacacc
     5461 gccaaatatt ccgtatgttc tcaccgacat atatccggtg agttggacga ttgcgtcttc
     5521 cacctgtttg accaattcac gagttggaac aaggattagg atggacggat gttttgctgt
     5581 actatattga tgcaggctga gtacaggaac caaataggca agggttttgc ctgttccggt
     5641 ttgcgcgatg ccgcctatgt ctcttccgga tttgacggct gaaaatacca attcctgaat
     5701 ggccgatgct tctgcaattc ccatatcgtc caatgccctt aggataggct ttggtatttt
     5761 catttcgttg aatagcattc ctgtaatttt ggggcaaagg tacaaaaaag ccgattaact
     5821 tataataggc tattattcag acggatataa gttgggcgtg agtgacttga ggcttcaggc
     5881 ttattattac ttccttcatt ctaatatgac ttccagaaat ttcccgtttt cgtaaaccaa
     5941 ttggccatcg gcatatatct ttcctccatc cttcatgtcg gatatcatat cccagtgtac
     6001 tgaactttca ttcttaccac cagcctggag ataagattga ccgatcgcca tgtgtaccgt
     6061 gccgccgatt ttttcgtcat aaagaatgtt ttttgttagc ctgtcaatgc ggtaattggt
     6121 gcctatggct gcttctccga aacggcgtgt cccttcaatc cgaaagacct cgttcaggat
     6181 atcgatatca ttgtcgcagg tccatgatgt gatatagccg tccctgacct ccaatcggac
     6241 attattgagc agatgtccct ggtatagaga aggcaaggta aacagtatat gcccgttgac
     6301 gctgttttct tccgggctgg tatagatttc gccggacggc atattggtct gtccatcact
     6361 gttaatccac cggcgtcctt ctgtcctgaa tgaaatatcc atatttgggc tgagatatct
     6421 aaattccttg caactgttga gtaaatctgt aattgattgc tgtctggcac ggacgttgag
     6481 ccactcctgt tccggattgt cggtgttcag gaaacaggca ttggccacaa aagaagtgta
     6541 ttcttcaagg gacataccgg ccagatcggc tccggcctga gtggggtact gacaaagatt
     6601 gcggcgcagg tttcgggtgg cagtacgttc aaaatataat ttgtggaatt gagcatttgc
     6661 ggctgctcta acttcattta atgttgaatt gggtttggat cggctttttg atgggaaagg
     6721 tgccctgata accaaatatg cctcaaaaga ttccattgct tccttgtata gaggattgat
     6781 atagccggct tgtgcattat gtccataatc attgaaatca tccgacttgc cttcccattc
     6841 taagatggct tccatgatga cacccatctg ttgacattga cgataaagtt cggctacaag
     6901 cggttctgca agtgttgtcg tactgaccag taagcgctcc cctgatttca gttccatgca
     6961 ataattgagc aaaagcgaag catacttttt gataaatatg atattcattg tatttctaat
     7021 ttgttaaaac tggatattgg catcaaattt agccctttaa atttcccaaa ttcattctaa
     7081 ttcaataaat ttacgtttgt attttatctt ttgaagcaat tttgtaatga aagcaatact
     7141 tacaatccta atcatgttct ttgcctttca tctgtgttca gcccaggata taatccaggc
     7201 gcagcgggac caggaagcta agattaaaaa aatttataat cagaagaaaa tcaccgaact
     7261 ggaatattcc aaactgatga aggaccaaaa caacatcaag gacgccattc aaatcgccaa
     7321 ggcagatggg gtaatgactc ccaaggaaaa gaatgcaatc aataaaaaat tgatgcatgt
     7381 ggagaaaaag atcatcaagt atcaaagaaa tagcgaaatc tattgatgta gatccgatgg
     7441 catgacggat ttaagcggac tttatctttg gagacaggcg gaataggagt taaccggaat
     7501 taggtagatg tttaataacc ttaggcgttg ttgttgactt atttcccggg caaggaactt
     7561 ataaaaccta tccgatagaa tcgaaatctg attcatttac ctttggaatt ctttttaata
     7621 ggttcattca cttattaatc atctacctac ttgggttttt acatgtcatc atcggttcac
     7681 cggatttttc ctgcaatttt aatggccata acggattttt gagcatgagc gttaacgtcg
     7741 atactgcata gttattaaca ttattttgtg ggtaactaac atttttttat cgatataata
     7801 tcttggtatt taacgatata gttaagttat taacatgtgt tagtaagttt cgaatgagca
     7861 atttgtgtaa taagtatctt tgtaatgtca attgggacta taacctaaat ggagaataat
     7921 agtttcccgg tgactaaaaa caaattttac gttcaagcgt aaaactcata gaccgattta
     7981 agggaggcag tccgagaggc tgcctttact tttttcatga tgagtattta atctgtgtcg
     8041 ctctttacag gcctgtgatc cttctatcac tgatacgaaa aggtggtgaa agaaaaattc
     8101 cgaatatgtc ttaggtatga aatttgggga attattatag taaaaccaaa aaatgattga
     8161 ctttatttgg tgcaatgcta taaaatgatt ttttttgtgg aataaaataa acagctggat
     8221 catgtctgac aatattgaaa atgtaggaaa aaaaggatac tgtttcttat acttctttgt
     8281 tttatttttt gctatagtag cgttggtggt gtacctgtac aagtacaact tcaccatgaa
     8341 attctaagtt ggttgcacgg ccttgctcca aggcaatttc ccattcgatg cttatctggt
     8401 tgaagcgacg tcggaactct gtattattcc gttcattacg cacagtttta atgaatattc
     8461 cgcttaacgc cgaaatgcaa tagcgtgctg tcaaaccctc aataaaaatc aggttaacgc
     8521 ttcagaaata ggggcgtgta atctcatgtg attg
//
LOCUS       DAOQQN010000098         8502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3220_length_8502_cov_5.128093, whole genome shotgun sequence.
ACCESSION   DAOQQN010000098 DAOQQN010000000
VERSION     DAOQQN010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8502
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3220_length_8502_cov_5.128093"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1805)
                     /locus_tag="PLG24_05180"
     CDS             complement(<1..1805)
                     /locus_tag="PLG24_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ24315.1"
                     /translation="MKNALLLLIFCSVNTFIYAQNRTISGTIMDADTKGPIEFCNVFQ
                     SKDQFATISDENGRFNLLISASVHKSFLIFHSIGYRPDSIPIRPDKDNYQIFLHAENL
                     LLEDIVITGATRATLIKENPVSITAISRKQIEKATESNIIDALVKNVPGLNAVKTGPN
                     ISKPFIHGLGYNRVLTIYDGMRQEGQQYGDEHGLEVDHYNIDKAEVIKGPAGLLYGSD
                     AIAGVISLFPVIPTGNEGTFYGKYLSEYQTNNNLIGNGVTLNFSRERLLFALRGSYRT
                     AKNYRNPTDGRVYLTNFNESNFSAFAGFKSKKGYTHLNFTLYDNNQGIPDGSRDSLTR
                     KFTKQIFEGENDDLKTRPIVSDKELNTYKVPDLAQHIQHYRIYVQGFYQIGKGEVDYL
                     LGGQQNTRREYTHPSMPQQAGMYMRLNTLNYGIRYNAPKFANIETSLGVNGMLQNNKN
                     KDATDFPIPDYHLYDGGMYLYARIKKGKWSVSGGVRYDLRLVQWNDFYVGINPETGFE
                     HQTHANDSKANLQFPEYQNSFHGISGSIGTTYKISPNINLKANIGRAYRSPNITEIGS
                     NGLDPGAHIIYLGNRNFKPEFSLQEDFSISLRYKN"
     gene            1894..2550
                     /locus_tag="PLG24_05185"
     CDS             1894..2550
                     /locus_tag="PLG24_05185"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HMZ24316.1"
                     /translation="MVSQTEENYLKALFHLTYETENKSEAGTNELATYLEVKPATAND
                     MLKKLKDKGLVSYEKYGKITLTNKGSNIAIEIVRKHRLWETFLYEKMNFTWDEVHEVA
                     EQLEHIKSQKLVEQLERFLDFPEIDPHGDPIPNARGEVRKIVRKSLSEVCIGDVCKLV
                     AVKDGSVAFLQYVAKLGLGLSTKIKVVSRQEFDGSMEIEVNNQHFTVSQKFANNLYVV
                     "
     gene            complement(2735..3730)
                     /gene="tsaD"
                     /locus_tag="PLG24_05190"
     CDS             complement(2735..3730)
                     /gene="tsaD"
                     /locus_tag="PLG24_05190"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HMZ24317.1"
                     /translation="MPVTILAIESSCDDTSAAVVRDGKVLSNVVSSQDQHALWGGVIP
                     EVASRAHLRLIMPAVNKALKDASVDLKDIDAIGITRSPGLLGSLHVGVTFAKSLALAL
                     NIPLIDVHHMDAHIMANFIEAPVPSFPFLCLTVSGGHTQISIVRDYLDVEVIGATIDD
                     AAGEAFDKTGKILGLDYPAGPIIDKLAAQGNPVFSFPEPNIPGLDFSFSGLKTSILYF
                     LQKATTDNPAFIRENITDLCASVQSRIVSILISKLQKAVHQTGIREVALAGGVAANSG
                     LRKAMETMHEQEGWNIYIPRIAFCTDNAAMIGQNAYYKYLAGIFAGQDMEASARG"
     gene            3821..4657
                     /locus_tag="PLG24_05195"
     CDS             3821..4657
                     /locus_tag="PLG24_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HMZ24318.1"
                     /translation="MPQLITEYKHPVSGVISTYGSNLLLLSENNYLAYCIVAEDGRYE
                     LLREYRIATGEKFTDFHRAVLATDEYLGNAFRSVSIGISNKRMVLVPGAVFNREESVR
                     YLESGAPVWSTDEVKADYLEKQDIHLVYAFRSAIYQAYRNAFPNMKLENALSTYIKRL
                     EAIVALHDLGYKVFVNTLHDTMYIVVYRNRQLCYANVYEFATAEDFLYYIMLIYKEFQ
                     LDPESVPTYLSGKIDINSTLHDLLKRYIRQPEFIQALAEPSLVIDNLPAHVYFDLSSL
                     NV"
     gene            4654..5190
                     /locus_tag="PLG24_05200"
     CDS             4654..5190
                     /locus_tag="PLG24_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HMZ24319.1"
                     /translation="MRIISGRFGGRHFYPPADKWPTRPTTDIAKEGLFNVLNNRLDWE
                     SVKMLDLFGGTGNHCFEFISRGCENTTYVDKFVGCIRFVHDLKKKLLLDKELTVVKSD
                     VFKYINSCTERYDYIFAGPPYPLHNIPDIPDLIFEKELLNPEGLFVLETNQMHNFRDH
                     VHFKELRKYGTTYFWFFE"
     gene            complement(5318..>8502)
                     /locus_tag="PLG24_05205"
     CDS             complement(5318..>8502)
                     /locus_tag="PLG24_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24320.1"
                     /translation="SFDMTDALIGTSPDDRKNGSKSGRIRALGSITQLDNFTSGISNV
                     DVYHAKFGTDANSTWKLSYSTDNGSTWMQAGSDVTTSSTTLTKQTFTINQSGNVRIKI
                     EKLSTSANSNRTNIDDITVYSYSPPCTPPSTYPTSLAVGNVTTTTLDVSWTNTNNDNI
                     IVLAHKTSAVDADPANGTGYTSNAAFGSGDQVGTGNYVVYIGTSTNVTVTGLEEGTTY
                     HFKAYSYDNVSNCYNTTNTTSGSGTTNVTPPCTPPATYPTGLTTSNLAPTSMDVTWTN
                     TAGDDIIVLAHAGAAVDADPVNGQTYFGNAAFGIGSQIGTGNYVVYIGTGTSVSVTNL
                     TANTTYHFAAYSFNSVSKCYNTTNPATGSGMTTSPPAGDYRSKATGAWEDASTWEFST
                     DNGMTWQNAAQAPSSTDGTITIQNSHKVTFSASRTVDQLTINAGGELEVMTQTLTIAD
                     GTGTDLIINGTLTYTGGTITLGAGATGEVNNGGTYNYNENNTVKAPALTWNTGSTLLV
                     SRVGSSTTIHSSMAGQQLYNLVWNTTSQTSTVNFEGQITTINGNFSLIKGGSTNKGIR
                     LTSGSASQDLTLTIGGNLNISADQIFEFSNGNKNATVILNGNLNNSGEFYTNTKGGTS
                     LKGRLEVKGNINSTGIIRSSGTSVDNKIVLNGSSLQDVQIADELKPYSLGGTDKLTLE
                     IDNAAGARLLADLRFKGSSVVFTNGHIYTEGKTFDLRSGTITSTPTAYFVTASSSGTY
                     YATGGLRQGFPTVTSSNFYPVGPSYTLYMPATVTMTAGMANDSFDVRIVPLGEHGVMP
                     IDANKCIKYQWEISHKNATGSANLKLQWASGTEGTTFNPNVGLYIGHWKTSANKFTPI
                     VEATYTSGDPSATSNTPFSEFSPFVIASEFIALPVQWLDIKAMRRDSRVLLQWKTASE
                     RDVKNFEVERRSADGSFTTIGTVMPQNKPEINAYSFVDERPSSGINYYRVRQVDANGQ
                     YSYSKVVQISFVDGVSFGIRNLGQQSFGFTGINGKAVMKVYDINGRLLLTKSVTNDDR
                     EFLLGFSSGSYAVTLEEGDNFYSGIFLLSR"
BASE COUNT         2257 a         1821 c         1954 g         2470 t
ORIGIN      
        1 atgtttttat acctcaggct gatactgaag tcttcctgaa gtgaaaactc aggcttgaag
       61 tttctgttac ctaaatagat gatgtgtgcc ccgggatcaa gaccattact tccaatctcc
      121 gtgatattgg gcgaacggta ggctctgccg atgtttgctt taaggttgat gtttggggat
      181 atcttatagg ttgttccaat gcttccgctt atcccatgaa aagaattttg atattcaggg
      241 aattgcagat tggcttttga atcattggca tgggtttggt gttcgaaccc tgtttcagga
      301 tttatcccaa cataaaaatc gttccactgg acaagtcgta aatcatagcg aactccgcca
      361 ctcacactcc attttccttt ctttatcctg gcatataaat acatgccccc gtcatataaa
      421 tggtagtccg ggatcggaaa gtcagtcgca tctttgtttt tgttgttttg caacattcca
      481 ttcacgccaa gcgaggtttc aatatttgca aattttggtg cattgtagcg gattccataa
      541 tttagggtat ttaggcgcat gtacattcca gcctgttgag gcattgaggg atgcgtatat
      601 tctctacggg tattttgttg tccgcccaat aaataatcta cctctccttt tccaatttga
      661 tagaaacctt gtacataaat tctgtaatgc tgaatatgtt gtgccaaatc cggcactttg
      721 taggtattca actccttgtc agatactatt ggccttgtct ttaaatcatc attttcccct
      781 tcaaaaattt gttttgtgaa ttttcgggtc aatgaatccc tgcttccatc cggtattccc
      841 tgattattgt cgtaaagggt gaaattcaaa tgcgtataac ctttttttga tttgaatccg
      901 gcaaaagccg aaaaattact ttcgttgaaa ttagtcaaat agacccggcc atccgttgga
      961 tttcgataat tttttgctgt tcgatacgag ccacgcagtg caaacaatag acgttcgcgg
     1021 gaaaagttga gggttacacc attcccgatc agattattgt tggtttgata ttctgagaga
     1081 tattttccat aaaacgtccc ttcattaccg gtgggaatta caggaaataa actaatgact
     1141 ccggcaatag cgtcacttcc atataataaa ccggccggcc ccttaataac ttccgcttta
     1201 tcaatattat aatgatcgac ctctaagcca tgttcatctc cgtattgctg gccttcctga
     1261 cgcatgccat catagattgt gagtacgcga ttgtagccta aaccgtgaat gaacggtttt
     1321 gaaatattag gccctgtttt taccgcattc aatcccggaa cattttttac caatgcatct
     1381 ataatattgc tctcagtagc tttttctatt tgtttgcggg aaatggcagt gatagagaca
     1441 gggttttcct taataagtgt tgctcttgta gcaccggtaa tgacaatatc ttctaaaagc
     1501 aggttttcgg cgtgtaaaaa aatctggtaa ttatccttat cgggccttat cggtatgctg
     1561 tccggcctat aaccaatgct atgaaaaatc aggaaagact tatgtacaga cgctgaaatc
     1621 aagaggttga atcttccgtt ttcgtcagag attgttgcga actgatcttt ggactggaaa
     1681 acattacaaa actctatcgg gcccttagtg tcagcatcca ttattgtccc tgaaatggtt
     1741 ctgttttggg cgtaaataaa agtattcaca gaacagaaga tcagcagaag cagagcattt
     1801 ttcatatttg tatttatatg tttcatttgc aaaattataa aagttagata tatctaacta
     1861 taaatattag aaaaatgttt ttatttttgt actatggtat cacagacgga ggaaaattat
     1921 ttaaaggctt tgtttcatct aacttatgag actgagaaca aatcggaggc cggtacaaat
     1981 gagctggcca cataccttga agtcaagccg gcaactgcca atgacatgct caagaaatta
     2041 aaagataaag ggttggtttc ttacgagaaa tacggaaaaa ttactttgac caacaaagga
     2101 agcaacattg caatagaaat tgtgagaaag caccgattgt gggaaacatt cttgtatgaa
     2161 aaaatgaatt ttacttggga tgaagtccac gaagtagcgg aacaactgga gcacattaaa
     2221 tctcagaaac tggtagaaca attagaacga tttttagatt ttccggaaat agatcctcat
     2281 ggagacccca taccgaatgc aaggggagag gtaaggaaga ttgttcgtaa atcactttca
     2341 gaagtctgta ttggagatgt atgtaagtta gttgcggtaa aagatggtag tgtggcattt
     2401 ttacaatatg tcgctaagtt agggttaggg ctgagcacaa aaataaaggt tgtcagtcgc
     2461 caggaatttg atgggtcaat ggagatcgag gtcaacaatc agcattttac ggttagtcaa
     2521 aagtttgcaa ataacttgta cgtcgtctga atgttaagta ggtagtgtaa ttgccattac
     2581 aataagatga agaagcggga gatgttttag ttgtgtaaat gcaagtatgt tgggctgtta
     2641 tccgggatat ttcggcagcc tctttatggg ggatgaattg ccataagtga atttgcaaaa
     2701 aagttaattt tatttccggc gataaagtcc ctgctcaacc ccgggcgctc gcctccatat
     2761 cctgacctgc aaagattccc gcaaggtact tataataagc gttttggccg atcatagcgg
     2821 cattgtctgt gcagaaagca atccgcggta tatagatgtt ccagccttcc tgctcatgca
     2881 ttgtctccat tgccttcctc agtccgctat tggcagccac acctccggcc agggctacct
     2941 cccggatgcc tgtctgatgt acggcctttt ggagtttgct gatcaggatg cttacgatac
     3001 ggctttggac agaagcacaa agatcggtaa tgttttcccg gatgaaggcg gggttgtcag
     3061 ttgttgcctt ttgaagaaaa taaaggatgg atgttttcaa tccgctgaag ctgaagtcca
     3121 ggcccgggat gttgggttcc ggaaaggaaa acaccgggtt gccctgtgct gcgagcttat
     3181 cgatgatggg gccggcggga taatcaaggc ccaggatctt accggtcttg tcaaaagctt
     3241 cacctgcggc atcgtcaatc gttgcaccga tgacttccac atcgagataa tcccgcacga
     3301 tactgatctg ggtatgcccg ccgcttaccg tcagacagag gaatggaaat gaaggtacag
     3361 gtgcttctat gaaattggcc atgatgtgag catccatatg gtgtacgtct atcaggggta
     3421 tattcagtgc cagtgccagg gacttggcaa aggtgacccc cacatggagt gagcccagca
     3481 agcccggaga gcgggttatg ccgatagcgt cgatatcctt caggtccaca gatgcatcct
     3541 tcaaggcctt gttgactgcc ggcatgatta gtctcaaatg cgcccgggag gccacctcgg
     3601 gaatcacacc accccaaagt gcgtgctgat cctggctgga cacgacgttg gacaatacct
     3661 ttccatcacg cacaacggct gctgatgtgt catcacagga gctttcaatc gctaaaattg
     3721 ttaccggcat aacaaaaaaa tgaagtgcaa atttacaaga ttgattataa gtacgccaaa
     3781 ttgtattaat attgtgtctt cggaaccata tttgatcata gtgccacaac tcattacaga
     3841 atacaaacat cctgtttccg gcgtcataag cacttatggc agcaacctcc ttttgctcag
     3901 tgagaacaat tacctggctt attgtatagt tgcagaggat ggacggtatg agttactcag
     3961 agagtacagg attgccacag gagaaaaatt taccgacttc catcgggcag ttcttgcaac
     4021 ggatgaatat ctgggcaatg ccttccggag cgtatccatc gggatttcca acaagcgaat
     4081 ggttttggtg cccggggcag ttttcaaccg ggaagaaagt gtccgatatc tcgaaagcgg
     4141 cgcccctgta tggtcaaccg atgaggtcaa ggccgattat ctggaaaaac aggacattca
     4201 tctggtatat gcctttcgtt ccgctattta tcaggcatac cgcaatgcct tccccaacat
     4261 gaaacttgag aatgccctaa gcacctatat taagcgactt gaagccatcg ttgccctgca
     4321 tgacctcggc tacaaagttt ttgtcaatac cttgcatgat accatgtata tcgtagtgta
     4381 tcgcaatcga caactttgct atgccaatgt atatgagttt gcaacggctg aagactttct
     4441 ttattatatc atgctcatat ataaggaatt tcagcttgac ccggaaagtg taccgaccta
     4501 tctttccggc aaaattgata tcaattcgac cttacacgac ctgttgaaac gctatattcg
     4561 ccaaccggaa tttatccagg cattggctga gccttcgctt gtcattgaca accttccggc
     4621 acacgtgtat ttcgacctgt catcccttaa tgtatgagga taatttccgg aagatttggc
     4681 ggcagacatt tttatccacc tgcagataaa tggcccacga ggccgacaac cgatattgcc
     4741 aaagaaggat tgtttaatgt gctcaacaat cgactggact gggaatcggt aaaaatgctt
     4801 gaccttttcg gtggtaccgg aaatcattgt tttgagttca tctcccgagg atgtgagaat
     4861 accacttatg tcgacaaatt tgtgggatgt atccgttttg tccacgatct gaagaaaaaa
     4921 ttgctattgg acaaggaact caccgtggtg aaatccgatg tattcaaata tatcaacagc
     4981 tgtacggaac gatatgacta tatctttgcc ggtccgcctt atcctctgca caatattccg
     5041 gatattccgg atctgatctt tgaaaaagaa ctcttgaatc cggaggggct gtttgtcctg
     5101 gagaccaatc aaatgcacaa cttccgggac catgtgcatt ttaaggaact tagaaaatat
     5161 gggacgacct atttctggtt ttttgaatga gaggagtcag aatagttgac caggcccctt
     5221 ttgaatttac cgtaaaatcg atgagacagc caaaacaaaa aagggaacct gcaggctccc
     5281 tttcttgttt gcttatatcg atcgcgagat agccctgtta tctgctcaac aggaagatcc
     5341 cggaatagaa attatctcct tcttccagag taacggcgta tgatccgctg ctgaacccta
     5401 aaaggaactc cctgtcatca ttggtgacag attttgtaag caataggcga ccattgatat
     5461 cataaacctt cataacagcc ttaccattta taccggtaaa tccgaaggac tgttgtccca
     5521 ggttgcgtat gccgaaggac actccatcca caaaactgat ctggacaact ttggaataac
     5581 tatattgtcc atttgcatcc acctgacgaa ccctgtaata gttgattccg gaggatggac
     5641 gctcatcgac gaagctgtac gcattgattt caggcttgtt ctgtggcatg acagtaccga
     5701 tagttgtaaa actgccgtcg gctgacctgc gctccacctc aaaatttttg acatctcgct
     5761 cacttgcagt cttccactgg agaaggaccc ggctgtctcg acgcatggct ttgatatcca
     5821 accattgaac cggcaaggcg atgaattcag atgcaattac aaatggtgag aattcgctga
     5881 acggtgtatt ggatgtcgca gaagggtcac cggaagtata tgttgcctca acaattggag
     5941 taaatttatt ggcactggtc ttccaatgac cgatgtagag cccgacattt ggattgaagg
     6001 ttgttccttc tgttccggat gcccactgca atttcagatt ggctgaaccg gtcgcgtttt
     6061 tatgactgat ttcccattga tacttgatac acttatttgc atcgatcggc attacaccgt
     6121 gctctcccaa tggcacaata cgtacatcga atgagtcatt ggccataccg gcagtcatgg
     6181 tgacagtagc cggcatataa agagtgtagg atggtcctac cggatagaaa ttggaggaag
     6241 taacagttgg gaatccttgt ctcaggccac ctgtagcata ataagtaccg gatgatgacg
     6301 ccgtaacgaa ataagctgta ggcgtgctgg taatggtgcc ggaacgcaaa tcgaatgtct
     6361 ttccttcggt atagatgtga ccattggtaa agacaactga cgaacctttg aatctcaaat
     6421 ctgccaacaa gcgtgccccg gccgcattgt cgatctcaag ggttaatttg tcagttcctc
     6481 caagagaata gggctttaac tcatcggcaa tctgcacatc ctggagggaa ctgccattca
     6541 aaacaatctt attatcaacg cttgttccgc tggatctgat gatgcccgta gagttaatat
     6601 ttcccttcac ttcaagacga ccctttaatg atgttcctcc ttttgtattg gtataaaatt
     6661 caccgctgtt gttcaggttg ccgttcagga tcacagtagc attcttgtta ccgttggaaa
     6721 actcaaaaat ctgatcggca gagatgttca ggttaccccc gatggtcagg gtcagatcct
     6781 gagatgctga tccggaagtt agcctgatac ctttattggt cgatccaccc ttgatcaggc
     6841 taaagttgcc attgatggta gtaatctgac cttcgaagtt gacggtgctt gtttggctgg
     6901 ttgtattcca taccagattg tataattgct ggccggccat agaggaatga atggtagtgg
     6961 atgagcccac tcttgacacc agcagcgtcg aaccggtatt ccaggtcagg gccggtgcct
     7021 tgactgtatt attttcattg tagttgtagg tgcctccgtt gtttacctcg ccggtagcgc
     7081 ctgcacccag ggttatggtg ccgcctgtat aagtgagtgt tccattgata atgaggtcag
     7141 taccggtgcc atcagcgatg gtcaaggttt gggtcatcac ttcgagttca cctccggcat
     7201 tgatggtcag ttgatctacg gtgcggctgg cactaaaagt taccttatga ctattttgta
     7261 tggtgatggt gccatcggta gaagaaggtg cctgtgcggc attttgccag gtcattccat
     7321 tgtcagtgga aaattcccat gtcgaagcat cttcccatgc tccggtcgct ttacttctgt
     7381 aatcaccggc tggcgggctt gttgtcattc cgcttccggt tgccggattg gtcgtgttgt
     7441 agcatttact gaccgaatta aaggagtaag cagcgaaatg ataggtggta ttggcagtca
     7501 gattcgttac gctgacgctt gtcccggttc ctatatagac cacataattg ccggtaccta
     7561 tctggctacc gataccgaag gctgcatttc caaaatatgt ctgtccattg acaggatcag
     7621 catcgaccgc tgcacctgca tgagccagga caatgatgtc atctccggca gtgttggtcc
     7681 acgtaacatc catagaggtt ggtgccaggt tggatgtggt caatccggtg ggataggtgg
     7741 ccggtggcgt gcagggcgga gttacattgg tggtaccact ccctgaagta gtattggtcg
     7801 tattgtagca gtttgaaaca ttgtcatatg aataagcctt gaagtgataa gtggtacctt
     7861 cttccaaacc tgtcacagtc acattggtgc tggtgcctat atatacgacg tagttaccgg
     7921 tacccacctg atctccactc ccgaaggcag catttgaagt ataaccggtg ccattggcag
     7981 gatctgcatc tacagcggag gttttatgtg ccaaaacgat gatgttatca ttatttgtat
     8041 tggtccatga aacatcgagc gtagtagttg tcacattacc tacggccaga cttgtgggat
     8101 aggtagatgg tggagtacac ggtggagaat aggaataaac cgttatatcg tcgatattgg
     8161 ttctgttaga atttgcggac gtagatagtt tttcaatctt gattctgaca ttcccggatt
     8221 gattaatggt aaatgtctgc ttggttaatg tagtactgct cgtggtgaca tcacttcccg
     8281 cttgcatcca ggtacttccg ttgtcagtgg aatagctcaa cttccaggta gaattagcat
     8341 ccgttccaaa ttttgcatga taaacatcca cattgcttat gccactagta aaattatcta
     8401 gttgcgttat agatccaagt gccctgatac ggccagattt acttccgttt tttctgtcat
     8461 caggtgaagt tcctatcaaa gcatcagtca tatcaaagga gt
//
LOCUS       DAOQQN010000099         8484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3226_length_8484_cov_6.308815, whole genome shotgun sequence.
ACCESSION   DAOQQN010000099 DAOQQN010000000
VERSION     DAOQQN010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8484
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3226_length_8484_cov_6.308815"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PLG24_05210"
     CDS             <1..792
                     /locus_tag="PLG24_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24321.1"
                     /translation="IKPGFMMWEDIKRVKINEIQQKEKDTPLDLLEELALTAPDSVSL
                     AEALINPLKSHTIAEFKRKSPIDGEMAPNADAMEVVSQFLQSGACAISIATDGSFYGG
                     NIDDLHLTKQIFREVPVIMREYIVDPYQIVEARALGSDAIILHGGLLTAEEVASFARL
                     AHALEMEVVFESSNEKDLKDYHPDVDIIMVHHRYNHEDVATPSKILSYLSQLPADKVR
                     VGGGGLKSYNQRKMLLDAGFDAVIIGTSLMKSKNHGDTLHSIIVG"
     gene            complement(904..1398)
                     /locus_tag="PLG24_05215"
     CDS             complement(904..1398)
                     /locus_tag="PLG24_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24322.1"
                     /translation="MRTIMNMLLVLGVLIPFAMNGQEMASNSGYKTFASSVGIEFIDR
                     ATDSDLNLYAGKIQYKDKCTAFTKMKKAGTFMTVFGSIALISGFIASNSDADFQNGGY
                     FLSDADIISFLGFTSGVLLTGAGIPLMIVGIVKSKNYCGSNGSTPRIILKSEGNSAGL
                     ALIF"
     gene            complement(1547..2044)
                     /locus_tag="PLG24_05220"
     CDS             complement(1547..2044)
                     /locus_tag="PLG24_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24323.1"
                     /translation="MKRCTTLLLLLGFLIPGTMFSQDLAQNNEYKTFASSAHLDYMDQ
                     ATESDLYLYSGNLMEKDKCRTYTKMKKAGLVMTIVGPIVMVGGVAMMLTGILTSDEEL
                     DVNTGLVVGGYVGIVAGALTTAGGIPLLIVGNVKSKQYCNGKKRNSQLNLNSGKNGVG
                     VALAF"
     gene            complement(2444..2989)
                     /locus_tag="PLG24_05225"
     CDS             complement(2444..2989)
                     /locus_tag="PLG24_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24324.1"
                     /translation="MKVHTIFLIISLLHAVNSYAQWPSNVMVLDPGEKSSIEVQGDLA
                     TGKVMEDLSWAWNSSNACFPETQASKFRGNHVFYAITMPTHCEMTISVVPTDNKGDLS
                     IYAYRIGASAYDLVPDLSRCITCEADYKWDKNWKGKVQTSERRVSMQNPSSNNYNILI
                     GVAGPASVTKGIFTLSVQFTR"
     gene            complement(3443..5644)
                     /locus_tag="PLG24_05230"
     CDS             complement(3443..5644)
                     /locus_tag="PLG24_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HMZ24325.1"
                     /translation="MRIILLLSFFFVFTRLDSQEVKSPSGQVTLTFQLIESVPTYRLN
                     YKDKEVIRTSRLGFELMDKGSAKNSFADEVIKDNSSDNRLDNLKDGFEVVGTTTSSFD
                     ETWQPVWGEERNIRNHYNELVINLIQTSTRRLMTLRFRVFDDGIGLRYEFPEQKNLIY
                     FTIREEKTQFVLAGDHTAFWIPGDYDTQEYDYNTSRLSEIRSLMSTAITPNASQTPFS
                     PTGVQTPLMLKTDDGLYINIHEAALINYGAMHLNLDDKQNILESWITPDANGSKGHLQ
                     APCHTPWRTVIVSDDARDILASRITLNLNEPCKIEDVSWIHPVKYIGVWWEMITGRSS
                     WAYTDDVPSVILGKTDYTKTRPNGRHAANTAHVKEYIDFAAEHGFDAVLVEGWNTGWE
                     DWFGNSKDHVFDFVTPYPDFDMAAIRDYARQKGVKMIMHHETSSSVRNYERYLDTAYQ
                     YMKTNGYDAVKSGYVGDIIPRGEYHYSQWINNHYQYALEKAAEYRIMVNAHEAVRPTG
                     ICRTWPNLIGNESARGTEYQAFGGSKPNHVTILPFTRLIGGPMDYTPGIFEMDISRIN
                     PNNHSHVNSTLANQLALYVTMYSPLQMAADLPEHYRQYPDAFRFIKDVAVDWDVSRYL
                     EAEPGEYITIARKAKNSDNWFVGGVAGNNGHTSLLQLNFLDADKVYIATLYMDGPDAD
                     YKTKPQSYIIKSGVVDQATKLAIKSAPAGGFALSLIAVEKKAVKGMRRLKN"
     gene            6725..7153
                     /locus_tag="PLG24_05235"
     CDS             6725..7153
                     /locus_tag="PLG24_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24326.1"
                     /translation="MRFYHNNGKLIAGFLVLFLFTISCSVKAQLKEMNPLSKIKIEIQ
                     NTKKGVTLKAMEGCAWSVLGFNLTEGQSQNIDNFGMVGQNDPLAVNTSSHSDFLITIT
                     KTREGFRLDGIKGTSWVALTFRLAVGKKSTFDDTGVWSED"
     gene            complement(7286..7855)
                     /locus_tag="PLG24_05240"
     CDS             complement(7286..7855)
                     /locus_tag="PLG24_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HMZ24327.1"
                     /translation="MVIDIILVVLIALAFYAGYNNGIFGISLKIVLFFGAILLALNLF
                     PIVFLFMENTFTTLTPVHYILGFVLVLATIFVLYRYISKKIESWVKANSMRLITRISG
                     GIIISLFVLLLFSFISGRLERWKIVKHEAFENSILHPAVDRIDQSFSALIRATGDIFG
                     KTFERNIKTINDIDSHKKEDPEKADTTKE"
     gene            complement(7950..>8484)
                     /locus_tag="PLG24_05245"
     CDS             complement(7950..>8484)
                     /locus_tag="PLG24_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HMZ24328.1"
                     /translation="DVKASSNLYATGWGHHLHPIFTTFHDDILIISKDEKSVSSTAGH
                     KLMENNPYAETRYMTVDTRLKRLITALKSGDVERFGAIVEQEAFGLHGLMMLSEPPYL
                     LMQPSTVAAIQRLMSWRSSTKIPLYFTLDAGPNLHLLYPDESASKVAAFIRSELLPFC
                     VEGSFIPDRVGNGPRQL"
BASE COUNT         2478 a         1801 c         1696 g         2509 t
ORIGIN      
        1 atcaagccag gatttatgat gtgggaagat attaaacgag tcaaaatcaa cgaaatacag
       61 caaaaagaaa aggacactcc actcgacctc ctggaagagc ttgcattgac ggctcctgac
      121 tcagtttccc tggcagaagc cctcatcaat cccctcaagt cacataccat tgcggaattc
      181 aagcgcaaat ctcccataga tggtgaaatg gctccgaatg ccgatgcgat ggaagttgta
      241 tctcaattcc tccaaagcgg cgcatgtgcc atcagcatag caacagatgg gagcttctat
      301 ggcggcaaca tagatgacct gcacttgacc aaacaaatat tccgggaggt tcccgtaata
      361 atgcgtgaat atattgtgga tccttaccaa atcgtggaag ccagagcatt aggaagcgat
      421 gctattatcc tccatggggg cttgctaact gctgaagaag ttgcatcctt tgcccgtctc
      481 gctcatgcac tggagatgga agtcgtcttt gagtcttcca acgaaaagga tttgaaagat
      541 tatcatccgg atgtggacat catcatggtg caccacagat ataaccatga ggacgtcgca
      601 actccttcaa aaatcttatc gtacctgtcc caacttcctg ctgataaagt cagggtcggc
      661 ggtggcggac taaagagtta taatcaaaga aagatgttgc tcgatgcagg ttttgacgca
      721 gtaatcatcg ggacatccct catgaaatcg aaaaatcatg gcgatacact gcattcgatt
      781 attgttggat aattgatgaa aaattggccc cgtagtggtt aatccataga ttgccagttg
      841 tcaaatcatc ctgcagggca ctcttccttc tcgaatgcat attgtctctt gtacggttaa
      901 atatcagaag atcagcgcta agccggcact atttccttcg ctttttaaaa ttattcttgg
      961 tgttgatccg tttgatccgc aataattttt tgatttaacg atgccgacaa tcataagcgg
     1021 aatcccggct cctgtcaaca agacgccgga agtaaagccc aaaaaactaa ttatatcggc
     1081 atctgataaa aaataaccgc cattttggaa atctgcatca ctgttggacg caataaaacc
     1141 ggaaataaga gcaatcgaac caaatactgt cataaaagtt cccgcttttt tcatcttggt
     1201 aaaagccgtg catttgtctt tatattgtat tttcccggca tataggttta gatccgagtc
     1261 tgttgcccgg tcaataaatt caatgccaac agaagatgca aatgttttat acccggaatt
     1321 gctcgccatc tcctgtccat tcattgcaaa tggaatcaat acgcctaaaa ctaaaagcat
     1381 gttcattatt gttctcatat tattttggct taattttcaa cttaaagata aaaaaataaa
     1441 ccctgattca attcaaatca ggggaggtta ttagattgcc aatacaaaaa aagggacgca
     1501 taataatgtt agcatccctc tttatgtcga atattttttt cattaatcaa aaagcaagag
     1561 ccacaccgac tccgttcttt ccgctattaa ggttcaactg agaattgcgt ttcttcccat
     1621 tacaatattg ttttgatttt acattgccca caattaagag tggaattcca ccggcagttg
     1681 tcaaagctcc ggcaactatc ccgacatatc cacccacaac gagtcctgtg ttaacatcca
     1741 actcttcatc acttgtcaat attccggtca acatcattgc aacgccgccc accatgacta
     1801 ttggtcctac tatggtcatc actaaaccgg cttttttcat cttggtataa gtcctacatt
     1861 tgtccttttc cattagattt cccgagtata aatacaggtc tgattcggtt gcctgatcca
     1921 tgtagtccag atgggcagat gaggcaaatg tcttatattc gttgttctgt gccaaatcct
     1981 gactaaacat ggtacccgga atcaaaaaac ccaagagtaa tagtaatgtt gtacatcttt
     2041 tcatgattat attgttttta ttttgggtaa atttaatata aaaaccttaa ttacaaaatt
     2101 tccgtaactt ttcagatcaa tttaatgctg ttctcttttg caataaattc aatcaacgac
     2161 aatttattac caatgctacc ttagcaacta ttcgattacg ctgtttcacg caatctattt
     2221 tgtgccgaaa agctcaacgc tattaggatt ttgaaggtaa gaaatgtcaa ttttcttata
     2281 atatttgttt gatgtatcta taccgacaac ctttatttga actaaaaaaa tcgtctttta
     2341 aatacaacaa cccactatgc aaccattcct ttcgggtgca gcctctgcat tgccgccacc
     2401 taggtataaa acaaggagtt ttttaattga cataagaaaa aacctaccgc gtaaattgca
     2461 cgcttaaggt aaaaattcct ttggttactg aggcaggacc tgccactcca atcaatatat
     2521 tgtaattgtt tgagcttggg ttttgcatac tgacccttct ttcactggtc tgaacctttc
     2581 ctttccagtt tttatcccac ttataatcag cttcgcaggt tatacagcga cttaagtctg
     2641 gcactaaatc ataggcggat gctccgatac gatatgcata gatactcaag tctcctttat
     2701 tatcggttgg gacgacactg atggtcatct cacaatgtgt cggcatagta attgcataaa
     2761 acacatggtt tcctctgaat tttgaggctt gtgtttccgg aaagcaggca tttgagctat
     2821 tccaagccca gctgaggtct tccatgacct ttccggtagc taaatcaccc tggacctcta
     2881 tgctactttt ctcccccggg tccaatacca tcacatttga tggccactgc gcataggaat
     2941 taacagcatg aagtaatgaa attatcaaaa atatggtatg tactttcata aataacaatt
     3001 taataaatta aaacaggtaa tcatttacaa cgggtaaaaa atggaaatcc attttcaatt
     3061 aaataccttt ccatctctta aagggtatat ccatgctaaa tacagtacta caaatttatc
     3121 ttatttcttg aaacaaaagt caaaaactcc ggaaatcttt cctgacaaat caaaaggtat
     3181 aagtaaaatc cggaaattga tctaaaaaag aatattagaa tttctgcagc aattccattt
     3241 catagccatt tcagttctca ccttccattg ttagaataac ctgccatcct taggaattgt
     3301 ggccggaact ccctcaacca tatagaataa ctattacgga atagcctatg gaccattttt
     3361 gtcgtcttga agcattggct acaggtgata aatgttaatt cttattgacc tgcaataccc
     3421 tttccggctt atacaccacc gatcaattct tcaacctcct catccctttc actgcctttt
     3481 tttctactgc gatcagggaa agagcaaagc ccccggcagg ggctgatttg attgccaact
     3541 ttgtagcctg gtctacaact ccactcttaa tgatatacga ctgtggtttg gtcttataat
     3601 ctgcatccgg accatccatg tagagtgtgg ctatgtacac cttgtctgcg tccaggaagt
     3661 ttagttgaag cagggaggtg tgaccgttgt tgcccgctac gcctcctaca aaccagttgt
     3721 cactgttctt cgccttacgt gcgatggtga tgtactcgcc gggttctgct tcgagatacc
     3781 ggcttacgtc ccaatccact gccacatcct tgataaagcg gaatgcgtcc ggatactgcc
     3841 tgtagtgttc aggcaggtcg gctgccatct gtagggggct atacatggtt acatataagg
     3901 caagttgatt ggccagcgtg gagttaacat gggagtgatt gtttggattt attctggaga
     3961 tgtccatttc aaagatgccg ggtgtataat ccatcggacc gccgatcaga cgagtaaagg
     4021 gcaatatggt gacatgattg ggcttactgc ccccgaatgc ctggtattcg gtaccacggg
     4081 ccgattcatt accaataaga ttgggccagg tacggcagat cccagtaggc cggaccgctt
     4141 cgtgggcatt gaccatgatt cggtattctg cagccttttc cagagcatat tgatagtgat
     4201 tgttgatcca ttggctgtaa tgatattcgc cacgaggtat gatatcgccc acatatccgc
     4261 ttttgactgc atcataaccg ttggtcttca tatactgata ggcagtatcc aggtagcgtt
     4321 cataattgcg tacagaggaa gaggtttcgt ggtgcattat cattttcaca cccttctgcc
     4381 tggcataatc gcgtatcgca gccatatcga aatccggata gggcgttacg aaatcgaaga
     4441 cgtgatcctt tgaattgcca aaccaatcct cccatcctgt gttccagcct tcgaccaaca
     4501 cagcatcaaa tccatgttct gcagcaaaat ctatgtattc cttgacatgg gcagtatttg
     4561 cagcatggcg cccattgggc cgtgtcttgg tgtaatccgt cttaccgaga atgacactgg
     4621 gcacatcatc cgtgtaggcc catgaactcc gaccggtaat catctcccac cagacaccga
     4681 tgtacttaac gggatgtatc caggagacat cctctatctt acaaggctca ttaagattga
     4741 gcgtaatacg ggaggcaagt atgtcccgtg catcatcact gactatcacg gtacgccatg
     4801 gtgtgtggca tggtgcctgg agatgaccct tactgccatt ggcatcagga gtgatccatg
     4861 actccaggat gttttgctta tcatccagat tgaggtgcat agcaccgtaa ttgattaatg
     4921 cggcttcatg gatattgatg taaagcccgt catcggtctt aagcataaga ggtgtctgta
     4981 caccggtggg cgaaaatgga gtctgagaag catttggcgt gatagccgtg gacatcaatg
     5041 atcggatttc tgaaagtctt gaagtattgt aatcgtattc ctgagtgtca taatctcccg
     5101 gaatccaaaa ggcagtatga tccccggcaa ggacaaattg tgtcttttct tccctgatgg
     5161 taaaataaat caggtttttt tgttccggaa attcatatcg taggccaata ccgtcatcaa
     5221 acactcgaaa tcgcaacgtc atcaacctcc tggtcgatgt ctgtatgagg ttgatgacta
     5281 gttcattgta atgattcctg atattccgct cttctcccca taccggctgc caggtttcat
     5341 cgaaggatga tgtggtagtg cccactacct caaaaccatc cttcaaatta tccagccggt
     5401 tgtcactact gttgtccttt attacctcat ctgcaaagct attctttgca gatcccttgt
     5461 ccatcagttc aaaccccagc ctactggtcc tgattacttc cttatccttg taattaaggc
     5521 gataagtcgg aaccgattca atcaattgaa aggtcaatgt aacctgtccg gaaggtgatt
     5581 ttacttcctg actatctaac cgggtaaaga caaagaagaa gctcagcaat aaaatgatcc
     5641 gcatgatgaa ataattttac gagtgcgagt ttaagcaaaa aaaatggaat tcaacgcatt
     5701 tgcacccatt atggaggttt cctctgttga atactcttgc atatccatgg gtttttgaag
     5761 cccgatcaga ttttttccta ttaaatgata tgatttcatg gtaagctgcc ttactgtaag
     5821 gttttagcgg agtattgatt tcaccaaggt aaacgattgg gtgaggattt gagaaaaatg
     5881 atcttaaagt ttttcctaca aaatacagat ttccattagg catcttgatt tcagtataag
     5941 tgcattatac tatacaatta tatgttggaa ggagatcggc ttcttcagtt aaataacctt
     6001 atcgatcagg gagattatcg cattgaagca taattactta acctcaatgg caaccatatt
     6061 ttccacctca ttcaaaccct tattgatatt aggtttctaa taagaatttt aaaaaaagac
     6121 acttccggtt tttagctttc aataaagcga gattactggc cccggcacaa attcatatta
     6181 attacccggc aattccaaat caaaataaat tgggcgctaa tcattttaaa atatacgtaa
     6241 aaagtatgac agctccttta ggtcaatatt tataaaacga agaaaaacaa taggctgatg
     6301 aaataaagga tacattatga aaaataaatt ccaaacgatg atgaggtgta aaattgaaga
     6361 caccctttat tggatagtct ttaacagatt aaaagttgat ccatattatc cttataagat
     6421 gaggttttcc ttgtcctatg acatttataa atggcaaaca acaatatgtc tacatggtgt
     6481 agatgtacat acctccgaaa ctttatttgt ttgcaaaggc acatagagga aaggttagtt
     6541 agaagaatat tgtcattcac cggcacctca tgcctgttac ttctaaataa atttataata
     6601 agagaaatgg tggtccttag gtctcaatac ctgatattcc ggtattgaga tggaatgctt
     6661 tttcatccac taataaagca aatattcatg tggatagctt atctttatca taaaacttat
     6721 gaaaatgagg ttttatcaca ataatggtaa attaatagca ggatttttgg ttttatttct
     6781 ttttacaata agttgttcag tgaaggcaca actcaaagag atgaatccat tgtcaaaaat
     6841 caaaattgaa attcagaata ccaagaaagg cgtaactctg aaggccatgg agggctgtgc
     6901 atggtctgta ctggggttca atctaaccga agggcaatct caaaatattg ataattttgg
     6961 catggttggt caaaacgatc ccttggcggt caatacatcc agtcattctg actttttgat
     7021 caccattact aagaccagag agggcttcag gctggatggc atcaaaggaa catcctgggt
     7081 tgcactgaca ttccgccttg ctgtcggaaa aaaatcaacc tttgacgata ccggagtttg
     7141 gtcagaagac tgagacgaca gcgtaaaatc atggtgaaat aatctttccg gtatcgaccc
     7201 ggcttttaag ggccaagaca taccgattcc tgtttttgta tcaaaaattt cctgagagag
     7261 agcgcgatgt gtgaccctgc tgctgttatt ccttcgtcgt gtcggccttt tcaggatctt
     7321 cctttttgtg gctgtctata tcgttgatgg tcttgatgtt tctttcaaat gtttttccaa
     7381 aaatatcgcc ggtggccctg attaatgcag aaaaactttg atcaatgcga tcaactgcgg
     7441 ggtgcaatat agaattttca aatgcttcat gctttacgat tttccatcgc tcgagtctgc
     7501 cggaaataaa gctgaaaagt aagagtacaa acagggatat gatgatgcct ccggaaattc
     7561 tggtaatcaa tctcatagaa ttggctttga cccaggattc aattttcttt gaaatataac
     7621 ggtataaaac aaagattgtc gccaaaacca agacaaatcc cagaatgtaa tgtaccggag
     7681 tgagggtagt gaatgtgttt tccataaaga gaaacacaat gggaaaaaga ttgagggcca
     7741 agaggatggc accgaaaaac agaacaatct tcagggaaat accgaaaatg ccattgttat
     7801 acccggcata gaatgccaat gcaatcagaa caacaaggat tatatcgatt accattcctg
     7861 gatttagttg ttttacaggt gaaagatagg cataatagcc gatatcaaat gcttgttttt
     7921 attgccttga attttatagt ctgtacatat tacaactgcc gggggccatt gcctaccctg
     7981 tccggtataa atgagccctc tacacagaag ggcaataatt cgctcctgat aaaggctgcg
     8041 actttggagg cagattcatc aggatatagg aggtgaagat tgggtccggc atcgagggta
     8101 aaatataatg gaatctttgt cgatgatctc catgacataa gtctttgaat ggccgcaacc
     8161 gtcgaaggct gcataagaag atatggtggt tcggacaaca tcatgagtcc atgcaaaccg
     8221 aaggcctctt gttctacaat ggcaccgaat cgttctacat cgccgctttt caaggccgta
     8281 atcaatcttt tgagtctggt atcaacggtc atataacgcg tctccgcgta aggattgttt
     8341 tccatcagtt tgtgtccggc ggtcgatgaa acactttttt catccttgct aatgattagg
     8401 atatcatcat gaaaggtagt gaagatggga tgcagatgat gtccccagcc tgtggcatat
     8461 agattggaag atgccttcac atcc
//
LOCUS       DAOQQN010000100         8439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3247_length_8439_cov_5.914241, whole genome shotgun sequence.
ACCESSION   DAOQQN010000100 DAOQQN010000000
VERSION     DAOQQN010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8439
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3247_length_8439_cov_5.914241"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..872
                     /locus_tag="PLG24_05250"
     CDS             207..872
                     /locus_tag="PLG24_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMZ24329.1"
                     /translation="MFVNEIINQNIPCLEPEQTGEDGLRLMEEYMVRHLPVVKEDMVI
                     GLVSEDMIYDWDTNTPFSEYIFRGYPQVIHHDSHILEAVEVIVKNNLTCVPVTDRNFK
                     YLGLISLQDAFQALSMEFALDEPGAILVLEINRLDYSLTEISRIVESEKGAILSSFIS
                     HPLDSDSNLVHVTLKISLQDIQFVKATFERYNYKVVATFSSVEYIDTLAERYDALMHY
                     LNI"
     gene            1390..4062
                     /locus_tag="PLG24_05255"
     CDS             1390..4062
                     /locus_tag="PLG24_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24330.1"
                     /translation="MRECYTKTFFLKNVIILTFLGFFSHQIKANGPVLPIKKADLTIE
                     VGSRNISCFNLIDGIAWVTASGGTPPYTYLWSNGATTDTIYNLPSNTYTITVEDFVGN
                     KVAAEVYIAEPPLLHSGVLLSNPILDCTTTQLTATATPIGGTPPCTFQWAFPQGVIDT
                     GQTVNIVQPYNYVYTVTDSRGCTFIGDTTIGSQNIPVVFLSLADTVSCNGLHDGSATS
                     SVTGGAPPYTYLWSNGSTNPNLLDVGAGSYSLTVTDTEGCSGQAYVFIPQPGALILSM
                     DGNDVTCFGQTTGAIFTTLTGGSLPYSYLWSNGETTPSLGNIGGGTYTLTVTDAHGCD
                     VVGSVTINQGNEIHIYTNTFPVSCNGGSDGYATVFATGGAGGFQYNWSNGTSGSQATN
                     LKAGTYLVYIIDKNQCVEERTLIITEPPLLEMSLNSTPEILGGSDGTAWADVSGGTTP
                     YTYNWSNGANTAIISGLTAGMYYLTVTDKKGCLKLDSIEVAASNCAFSAKLLTINPTC
                     FGGSDGAIFPEIITPGQEPYYFQWSDDTHDVILDSLKAGKYYVTIYDNANCMLTLSAT
                     LINPSRMNVDYILTQPDGPGKPYGSIKVFLNGGAPPYKVSFQGVDYLGGNEILIDNIP
                     PGNYTAYAKDSKGCSTPVDFQINQFECLLTGNVITVDEPDCYGDKNGQLCVMYQNNFG
                     SVSIEWSNGATTACISNLIAGEYAVVLRDTLGCMVVLESTVKQPNFITLENITINPGT
                     NHFDGSISLFVIGGTPPFTYTWTKNGEYFANTRNINQLNEGTYQLSVVDSKGCTALFE
                     AFRLTPSKSFSNYETGIKVYPNPVYENLIVETRNYTVIHSIDYISMNGKKVRLPFATT
                     DKGAVLDVGTLPQGPFVLSVVTNEGIANVKLVKM"
     gene            complement(4118..5755)
                     /locus_tag="PLG24_05260"
     CDS             complement(4118..5755)
                     /locus_tag="PLG24_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HMZ24331.1"
                     /translation="MRKVKIIGIVLVFAFVGMGAAVYNNDKLFEITKNLEIFSNLYKE
                     LNTYYVDDIDPNKLMKVGIDAMVETLDPYTNYFSETQIETWRYLSEGKYEGIGANVKN
                     IGDEITVIEPYEGYPAYEAGLKAGDVILSIDGQSTKGKNSNDLGVLLRGVPGSSVQLE
                     IKRPGVKEIKTFKINRGEIKMSNVPYSGIVRDHVGYVSLSTFTQDAAKNIGDAIREMK
                     KNDPQLKGLVLDLRDNGGGLLIEAVNIVNLFVGANVEVVSTRGKVRDWDKVFSTRGPA
                     LDEKLPLVVLINKQSASASEIVSGSIQDLDRGVVIGQRSFGKGLVQNTKDIGYNSKLK
                     LTTSKYYIPSGRCIQSVSYKNGEPVDIPDSQRHVFRTKGGRKVLDGGGVTPDIYVAPD
                     SGDAPIKALLDDFVVFNYVTKYMIAHPNIAPADQFVFNDFDDFLKYVESNNYPFTTAS
                     EKKFEELTNSARKDEFYAPLQSDLSALKMKIQKEKRNVLIKHKTQINTFITKDIVNRA
                     YGQHGKIINSINHDREVSDAINLLNDPTRYNKILKGK"
     gene            complement(5873..6373)
                     /locus_tag="PLG24_05265"
     CDS             complement(5873..6373)
                     /locus_tag="PLG24_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24332.1"
                     /translation="MRTVLSLVVLCLPCWLSMTVLHPFHVSNTEFNYNATDKNLEVSC
                     RIFTDDFEKALGMYFKEKADFTDKKLSGKMEIMVRDYMKTHLAIKIDNKRLALSCLGF
                     ELDHEAVNVYLEHPLALPPKKIEVQNTLVYEAYEDQVSVVHFIVNGQRKSSKLDYPDK
                     VLHFTF"
     gene            complement(6370..>8439)
                     /locus_tag="PLG24_05270"
     CDS             complement(6370..>8439)
                     /locus_tag="PLG24_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HMZ24333.1"
                     /translation="VITDALDMKGVTKNFQKGEIEVLALKAGVDVLLLSEDVPVSIAA
                     IKKALKDGKLTAERIDESVRKILEAKYRFGLTGSVNISTANLASDLVTGEARALNQKI
                     IEQAITMVKNADGLVPIGNIEKKISTLAIGASTKTPFQQQVDAYCKASHFFFNKETSS
                     SLSEKLSGMDLVIVGITGMNNSLKDNFRISKDAVALVNQLSKKTKVIVVVFGNPYGLS
                     NFVSPSTVICAYNDDALSQSLGIQAVFGGLPINGKLPVTALPYPFESGLQVSEATRIS
                     FGEPESVGMDSGTLAKLDELARDLVSRQAAPGCEMLVIRDGKVVYNKQFGRYTYDNRS
                     HQVEQYTMYDLASITKIAATTLGIMKLYEQGKMDVYKFLGSYLPELKGSNKEFMVIRD
                     VMAHRAGLFPWLPFYKETLVNDGFRTRTSAMIYDSKKSDEYSIKVANRLYMRSDFINT
                     LWNEIIRNQNLQSNDYKYSDLGFIMLRKAVENITKEPMDKYLESTFYKPMGLYSMTFK
                     PLEKFQKSDIAPSEDDRYFRQQVLQGDVHDMAAAMFGGVSGHAGLFSNSVHLGTLMQM
                     LLNGGSYGGKQYLKAETIKLFTTRHPADTRRGLGFDMPQTDWSMIQNVTAKASRNTFG
                     HSGFTGTVVWADPQSRLIFVFLSNRTYPTMDNNLLERRNYRIKAHAITYEAISKYDHT
                     DDLNASL"
BASE COUNT         2244 a         1811 c         1894 g         2490 t
ORIGIN      
        1 gaggcggttg tcgaaacggt tgagaaaact gaggtgccga atgtggaaca gagcgaatcg
       61 gtcccgttgt caaccgatga attgcttttc gaattgaaga agcttggaga aatgcggcaa
      121 aatggagtga ttaccgagga tgaattttta aaaaggaaac aaaagctcta cgcgtgacgt
      181 tttagtataa ataatactga ttgcaaatgt ttgtcaatga aatcataaac cagaacatcc
      241 cctgtcttga gccggagcaa accggtgaag acgggcttcg gcttatggag gaatacatgg
      301 tcaggcattt accggtggta aaagaagaca tggtcattgg attggtatct gaagatatga
      361 tctatgattg ggataccaac acaccatttt ccgagtacat tttcagaggg tatcctcagg
      421 ttattcatca tgacagccat attcttgagg ccgtagaagt gattgtcaag aacaacctaa
      481 cctgtgtgcc ggtgaccgac aggaatttta aatatctggg attaatatct cttcaagatg
      541 catttcaggc actaagcatg gagtttgctc ttgatgagcc gggtgcgatt ctggtactcg
      601 agatcaacag gctggattat tctctgaccg agataagccg catagttgaa tcggaaaaag
      661 gagccatcct ctccagtttt atttcgcatc cgttggattc agatagtaac ctggtccatg
      721 ttaccttgaa aatcagcctg caggatatac aatttgtcaa agccactttt gaacgctaca
      781 actataaggt ggtagccacc ttttccagtg tggaatatat cgacacgctg gctgagcggt
      841 atgatgctct tatgcattac cttaacattt aatttgcgga ggctgtttat ttcgataaat
      901 atgatgcctg cgggaatttt tttacaaaaa aaagaattta aatataaccc ggtcattcat
      961 aagggatggt tgcatttatg tggcctgatt ggccattttg atatctaccg gaaccggaga
     1021 acctgccggt cagtcatctg caccctttcc atggtttagg aggagaataa ttaagggtat
     1081 ataatatttg attggtcacc tgctttcatc aattgattcc accgttgccc ttttaatatt
     1141 gtaagttctg atcgatacgt acattatgga aatgggacac agcggtctta gcgaggtata
     1201 atttcaggtg tcatcaggta ctttgcctga atttttggaa cagaaattgt atgtataatt
     1261 atgaattgta atatatagag gatggaacac aatattgtat tcggtggtat tgcaataaat
     1321 atttaattat caaggattaa taaattgaaa taatttgttt ttgatatcgg gtaaacgaaa
     1381 agaacaacga tgagagaatg ctacactaaa acattttttc ttaaaaatgt aattatttta
     1441 acttttctgg gatttttttc acaccagata aaggccaacg gacctgtgct tccaatcaaa
     1501 aaagccgatt tgaccataga ggttggttcg cgtaatatca gttgcttcaa tcttattgat
     1561 ggcatagcgt gggttacagc ttcaggtggg acgcctccgt atacttatct atggagcaac
     1621 ggagccacca cagatactat atataacctt ccatccaaca cgtataccat aacagtggag
     1681 gattttgtcg gtaataaggt ggctgccgaa gtgtatatag cagagccgcc ccttcttcac
     1741 tccggtgtgt tgttgtccaa cccgattctg gactgcacga ctactcagct gactgcgaca
     1801 gccacaccta taggtggcac tcccccctgc acatttcaat gggcatttcc acaaggggtg
     1861 attgataccg gacagactgt caatatcgtg caaccttata actatgtata tacagtgaca
     1921 gattccagag gttgtacttt tattggagat accacgattg gatcgcagaa tattccggtc
     1981 gttttcttat cccttgcaga tacggtcagt tgcaatggtt tgcatgacgg aagtgcgacc
     2041 tcatcggtaa caggtggcgc tcctccttat acctatcttt ggtccaatgg atcaaccaat
     2101 ccgaatctcc ttgatgtcgg tgccggatca tactctctca cggtaacaga tacagaggga
     2161 tgttcggggc aggcatatgt attcataccg caacccggag cgcttatatt atctatggat
     2221 ggcaatgatg ttacctgctt tgggcaaaca acaggagcta ttttcacaac gctgaccggg
     2281 ggctcattgc cctattccta tttgtggtcc aatggagaga caacaccatc attgggcaat
     2341 atcggagggg gaacttatac gcttacggta accgatgcgc atggatgtga tgtggtcgga
     2401 tcggtgacta tcaaccaggg taatgaaata catatttata ccaatacgtt tccggttagt
     2461 tgtaatggtg gaagtgacgg ttatgcaacg gtttttgcca caggaggagc cggaggattt
     2521 cagtataact ggtctaacgg cacttcagga tcacaggcta ccaacctaaa agcgggaaca
     2581 tatcttgtat atatcattga taaaaaccaa tgtgttgaag aaagaacact gataatcaca
     2641 gaacctccac ttctggagat gagtctgaac agcaccccag aaatattggg tggcagtgat
     2701 ggaaccgcat gggcggatgt tagtggcggt acgacgccat atacttacaa ttggagtaat
     2761 ggtgccaaca cggcaattat ttccggtctg actgccggca tgtattatct gacagtgacg
     2821 gataagaagg gatgtctcaa actggattcc attgaagtgg cggccagcaa ttgtgccttc
     2881 tcagcgaaac tactcacaat caaccccact tgctttggtg gctcagatgg agcaatcttc
     2941 ccggagataa tcacaccggg ccaggaaccc tattatttcc aatggtcgga tgacacccat
     3001 gatgttatcc tcgacagtct taaagccggg aagtattacg tcaccatata cgataatgcc
     3061 aattgtatgc tgacattgtc ggctaccctg atcaatccga gccggatgaa tgtcgattat
     3121 atacttacac agcccgacgg tccaggcaaa ccatatggtt cgattaaggt ctttctcaat
     3181 ggaggggcac caccctataa ggtatccttt caaggggtag attatctggg tggaaacgaa
     3241 attttaatag ataatatacc tccgggtaat tatactgcct atgccaagga ttcaaaggga
     3301 tgctccaccc cggttgactt ccagatcaat cagtttgaat gtctgttgac gggaaatgtt
     3361 atcactgtgg atgagcctga ttgctatggg gataaaaacg gacagttgtg tgttatgtat
     3421 cagaataact ttggatcagt ctccattgaa tggtcaaatg gggcaacaac agcttgtatc
     3481 agcaatctga ttgcgggtga gtatgcagta gtgcttcgag acaccctcgg gtgtatggtt
     3541 gtccttgaat ctacggtcaa gcagccaaac ttcattacgc tggagaatat cacaatcaat
     3601 cccgggacta atcatttcga cggatctatt tcgctctttg ttattggagg cacgccgcca
     3661 tttacctata cctggactaa aaacggtgaa tattttgcca atacaaggaa catcaaccaa
     3721 ttgaatgaag ggacttacca gctcagtgta gttgactcta aaggctgtac ggcgctattt
     3781 gaagccttca ggctgactcc ttccaagagt ttttctaact atgaaacagg gataaaggtg
     3841 tatcccaatc ctgtctatga aaatttaatt gtagagacac ggaactatac agtcattcat
     3901 agcattgatt atataagtat gaatggtaaa aaggtgcggt tgcccttcgc caccaccgac
     3961 aagggagcgg tgcttgatgt aggcacattg ccgcaggggc cttttgtcct cagtgttgtc
     4021 acaaatgaag gtatcgccaa tgtaaaattg gtgaagatgt agtaattcta tgcttgaaca
     4081 atatgtaaag gcagaagtca catgacttct gcctttttta ttttcctttc agtattttat
     4141 tataccgggt gggatcattg agcaaattta tggcatcact tacttcccgg tcatggttga
     4201 tgctgttgat tatctttcca tgctggccat atgccctgtt gacaatatct tttgtaataa
     4261 aagtattgat ctgggtcttg tgcttgatca gcacatttct tttctctttc tgtattttca
     4321 ttttaagtgc tgacaaatcg ctttgtaatg gagcatagaa ctcatccttc ctggcagaat
     4381 tagtcaactc ttcgaatttc ttttcagaag ccgtggtgaa cggatagttg ttggactcga
     4441 cgtatttaag aaagtcatca aagtcattaa aaacaaattg atctgccggt gctatattgg
     4501 gatgggcaat catgtatttc gtgacatagt tgaaaacgac aaaatcatca agtagcgctt
     4561 tgattggagc atcacctgaa tccggagcca catagatatc cggagtcaca ccgccaccgt
     4621 caagcacttt tcttcctccc tttgtcctga agacatgtct ttgactatcc ggaatatcga
     4681 caggttcacc atttttgtat gaaacacttt gaatacaccg tccactggga atataatatt
     4741 tggaggtggt tagcttcaac ttactgttgt accctatatc cttggtgttt tgtacaaggc
     4801 cttttccgaa ggagcgctgg ccgatcacaa cacctctgtc caagtcctga atcgaaccgc
     4861 ttacgatttc agaagcagaa gcactctgtt tattgatgag gacgacgagg ggtaattttt
     4921 cgtccagtgc cggtccacgg gtagaaaaca ccttatccca atcccttacc ttacctcttg
     4981 tgcttactac ctccacattg gcaccgacaa agaggttgac aatattgact gcctctataa
     5041 gcaagccgcc cccattgtct cttaagtcga gtaccaggcc cttgagctgt gggtcgtttt
     5101 ttttcatttc acggatggca tcaccgatat ttttagcggc atcctgtgta aaggtcgaca
     5161 gagatacata accgacatga tcacgaacga taccagaata ggggacattg ctcatcttga
     5221 tttcaccacg gttgatcttg aaggtcttga tttcctttac accaggacgt ttgatttcca
     5281 actgtacgga agaaccgggc acgcctctca atagaacgcc taggtcattg ctgtttttgc
     5341 ctttagtgga ctggccatcg atggaaagaa tgacgtctcc ggctttcagg cctgcttcat
     5401 aggccggata accttcataa ggctcgatga cggtgatttc gtctccgatg tttttgacat
     5461 tggctccgat accctcatat tttccttcag ataaatatct ccaggtctct atctgggtct
     5521 cgctgaaata attggtataa gggtcgagcg tctcaaccat ggcatcgata ccaaccttca
     5581 tcagtttgtt cgggtcgata tcatccacat aataggtatt tagttcttta taaaggttgg
     5641 agaaaatctc gagatttttt gttatttcga atagcttatc gttgttatat acggcagcgc
     5701 ccatgcccac aaaggcaaat acaagaacaa ttcctataat ttttactttc ctcatttcgc
     5761 aagattaatt tttcttttca cataaaacgg atatccgact tcttttgata tcagcaacga
     5821 attttttgac caattgtttt ttgatacagg ttgatcatag tcctgatcat atttaaaagg
     5881 tgaaatggag tactttatcc ggatagtcca gcttgctgga ttttctttgg ccattgacga
     5941 taaagtgcac tacactgacc tgatcctcat aggcttcata tacaagagta ttttgtactt
     6001 caattttttt gggaggcaag gccagcgggt gttccagata cacattgacc gcctcatggt
     6061 cgagctcaaa tccgagacag gatagagcca gccttttatt gtcgattttg atggcgagat
     6121 gggttttcat ataatcccga accataattt ccattttccc ggacaatttt ttgtcggtaa
     6181 agtctgcttt ttctttaaaa tacatgccaa gtgccttttc aaaatcgtcc gtaaagatcc
     6241 tgcaggacac ctccagattt ttatcggttg cgttatagtt gaattcggtg ttgcttacgt
     6301 gaaagggatg taagacagtc atggacaacc agcaaggaag acaaaggacg acaaggctca
     6361 gcacagtcct cataaggaag catttaagtc atcagtatga tcgtatttgc tgattgcttc
     6421 ataagtgatg gcatgggctt ttatacggta atttcgtctt tccagcaggt tgttatccat
     6481 ggtcgggtag gtcctgttgg acagaaatac gaaaatgagt ctggattggg ggtcagccca
     6541 gactactgtt ccggtgaatc cggaatgccc gaatgtattg cggcttgctt ttgctgttac
     6601 attctgaatc atgctccagt cagtctgagg catatcaaat ccaaggccac gtcttgtatc
     6661 ggccgggtgt cttgttgtaa acaacttgat ggtttcggct ttaagatatt gtttgcctcc
     6721 gtaactaccc ccattaagga gcatctgcat cagtgtgccc agatgtaccg aattgctgaa
     6781 tagtcctgca tggcctgaaa ctcctccaaa catcgcagcg gccatatcat ggacatcgcc
     6841 ctgtaacacc tgttgtctga aatagcgatc gtcctcggag ggggcaatat cgcttttctg
     6901 aaacttttca aggggcttga aggtcatgct gtaaagaccc attggcttgt agaatgtgga
     6961 ttcaagatat ttgtccatcg gctcttttgt aatattctca actgctttcc ggagcatgat
     7021 aaatcccagg tcggaatatt tataatcatt gctttgcagg ttttgattgc ggatgatttc
     7081 attccatagg gtattgatga agtcgcttcg catatacaga cggtttgcaa ccttaatact
     7141 gtactcatca cttttctttg aatcgtagat catggcagaa gttctggtcc tgaacccgtc
     7201 attgaccaat gtctccttat aaaatggaag ccagggaaac aatcctgccc tgtgagccat
     7261 tacatccctg ataaccatga attccttgtt tgatcctttc aattcaggca ggtaagatcc
     7321 cagaaacttg taaacatcca ttttcccctg ttcgtatagc ttcatgattc caagcgtggt
     7381 agctgctatc tttgtaatgg aagcaaggtc gtacatggta tattgctcca cctggtgtga
     7441 acggttatca taagtatatc gtccgaattg cttgttgtaa accaccttgc catcgcggat
     7501 caccagcatc tcacaaccgg gtgctgcctg tctgcttacc aggtccctgg ccagttcgtc
     7561 cagcttagcg agcgtaccgg agtccatacc gacgctttcc ggttcgccga agcttattct
     7621 ggtcgcctca gacacctgga gcccactttc gaaaggatat gggagggctg tgactggcaa
     7681 tttaccattg atgggcagac cgccaaatac ggcttgaatc cccaaagact gtgataaggc
     7741 gtcatcattg taggcgcaga taaccgttga agggcttacg aagttactta gcccataagg
     7801 attgccgaag accacgacta tcaccttggt cttttttgac aattggttga caagggccac
     7861 tgcgtccttg cttatcctaa aattatcctt cagggagtta ttcatgccgg taattcccac
     7921 gatgacaaga tccataccgg ataatttttc tgacagagat gagcttgttt ccttgttaaa
     7981 gaagaagtga cttgccttgc aataggcatc aacttgctgt tggaaaggag ttttggtgga
     8041 agcgcctatg gccagtgtgg atatcttctt ctctatgtta ccgatgggga ccagcccatc
     8101 ggcattcttg accatggtga ttgcctgttc tatgattttt tgattgaggg cacgagcttc
     8161 tccggtgact aagtcggagg caaggttggc cgtggaaata ttgacggacc cggtcaatcc
     8221 gaatcggtac ttggcctcca ggattttccg aacactttca tcgatccgtt cagcggtcag
     8281 ttttccatcc ttcagtgcct ttttgattgc tgcaatggag acaggaacat cttctgacag
     8341 caggagtaca tccacacctg ctttcagcgc taatacctca atttcacctt tctggaaatt
     8401 ctttgtgacc cccttcatat caagggcatc ggtaatgac
//
LOCUS       DAOQQN010000101         8405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3275_length_8405_cov_5.904072, whole genome shotgun sequence.
ACCESSION   DAOQQN010000101 DAOQQN010000000
VERSION     DAOQQN010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8405
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3275_length_8405_cov_5.904072"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PLG24_05275"
     CDS             <1..197
                     /locus_tag="PLG24_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24334.1"
                     /translation="KGLGEMNAEQLWETTMDPSRRTLRQVTIEDAIECDRVFSMLMGD
                     EVPPRREFIEANATYAKIDA"
     gene            complement(286..1833)
                     /locus_tag="PLG24_05280"
     CDS             complement(286..1833)
                     /locus_tag="PLG24_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HMZ24335.1"
                     /translation="MNKELIINSLPSEVEIALMENHRLVEIHKQKTSNSYNVGDIYLA
                     KVSKLMPGLNAAFLDIGKRKDAFLHYTDLGPKLPSVIECTQQLVNKTPGFNIHNMDLS
                     KEINKNGKIQEVMDKKSMLLVQILKEPISTKGPRLSCEITIPGRYLVLTPFSNAVTIS
                     KKIINPEERKRLQLLIESIKPKNFGIIARTAAEGRKVADLHDEISNLAQKWQEIEQNL
                     SNAKGPKKVLSEADKTTSLLRDFLSDDYHSVVVNDKEVFNNIKSFMSSIAPEKVKIVE
                     HYNGKLPIFEHFGINKQIKASFGKTATMNSGAYLVIEHTEAMHVIDVNSGPKMSKSEM
                     NNTSLTVNLEAAEEITRQLRLRDLGGLIIIDFIDMKNPDHKKALNNRMVELMAQDRAQ
                     HTILPLSKFGLMQITRERVRPALKINTDEVCPACNGSGKVQPSILLTDEIERDLVYLL
                     TNKSISKVNLSVHPYVHAYLTKGLLNIRMKWYFRHKKWVNIRPNSNFALTEYRFFDIA
                     EDEIRFT"
     gene            complement(2078..2881)
                     /locus_tag="PLG24_05285"
     CDS             complement(2078..2881)
                     /locus_tag="PLG24_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24336.1"
                     /translation="MANNRPTGISRQKKIALGSAILLFCILYFSFSLTDKKAKARKDK
                     ERSLITEATGPEALLKEARLSASGDTLELVERLQHATEGKDTANILDNLKRISGIWYS
                     QRRYGLAGYYAEKVAELEKTATAWSIAGTSYGMGVKVLSEGSEREFCAGRAAKAFEKA
                     ISIEPDATIHRLNLAAIYADAPPKDDVMKGIKMLLDLNKKEPGNTAVINLLGELAIKT
                     GQFDKAIERLEESYRIDPDNPQTPCLLSMAYEGAGQHEKAHELAEKCRN"
     gene            complement(2883..3191)
                     /locus_tag="PLG24_05290"
     CDS             complement(2883..3191)
                     /locus_tag="PLG24_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HMZ24337.1"
                     /translation="MRKADLVATIAEKTGVAKVDVLVSIEHFFTEVKNTLSSGENVYI
                     RGFGSFIIKKRAKKIGRHIKKNEAIEIPEHFIPAFKPAKTFTEQVKNRVTVAPEVEED
                     "
     gene            complement(3378..4148)
                     /locus_tag="PLG24_05295"
     CDS             complement(3378..4148)
                     /locus_tag="PLG24_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HMZ24338.1"
                     /translation="MLHINLRHLRRKKQMSQQNFADVLGIPRTTYSEYEKGNTEPNLK
                     MLGRMCEALDVTLDQLVKADLTMDNLEVFRNKELRVLAMTVDTEGRNQIELVETRAEA
                     GYMSGMADPEYISDLPRISVPSLQGGRYRAFEISGDSMLPMESGSIIICRFVESLRDI
                     RDDKTYIIITHKDGVVYKRVRKADEEARLIAISDNERFMPYTIAYEDIQEIWQYHAHI
                     GFSDVKHTFRTMMEEKIEDIARKVNELHAASIKKFDKN"
     gene            4626..5825
                     /gene="lysA"
                     /locus_tag="PLG24_05300"
     CDS             4626..5825
                     /gene="lysA"
                     /locus_tag="PLG24_05300"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HMZ24339.1"
                     /translation="MNNKDLLKIAEQFGTPTYVYDAESIKAQYDKLVGAFSTDTRFFF
                     ACKALSNINILKYIRSLGAGLDCVSINEVMLGLRAGFGPEKILFTPNCVDLAEIEEAI
                     CHKVHINIDNISILEQFGNKYGGSYPIFIRINPHIFAGGNYKISTGHIDSKFGISIHQ
                     LRHIERLVKSTGVNIEGLHMHTGSEIKDADVFIEGLEIMFELAEHFPNLKYLDMGSGF
                     KVAYRDGDMETDVAELGQKVAAAMDRFASDTGKKPELWFEPGKFLVSKSGHFLVRTNV
                     IKHTTATVFAGINSGFNHLIRPMFYDAYHEIQNLSNPSGPERIYTVVGNICETDTFAW
                     DRKLNETREGDILVFRNSGAYGFEMSSNFNSRLKPAEVLFLDGKIHLIRRRDAFEDLL
                     KNQIEVL"
     gene            5984..6670
                     /locus_tag="PLG24_05305"
     CDS             5984..6670
                     /locus_tag="PLG24_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24340.1"
                     /translation="MQQDLHLAGLIISEPTTFLTNLLLAVTGIYLGSRFLSGRHAGPE
                     FIRQYGLFLMLTGISALWGGVFSHGLKVYFAVEYSIPGWITAFVAAYYMAGGAISHLQ
                     YCNLAPFDNDIVVRRIKMVNLLILLSAIIFIFIKPHFIVVAVNAAIALVAAGLFCEWI
                     AYKNTGDKGSFLFLVAVTISILTILLFLFKINAEPWFNTNDLSHLVMIGCMFIFAKGF
                     EQMKINIVSA"
     gene            6667..7599
                     /locus_tag="PLG24_05310"
     CDS             6667..7599
                     /locus_tag="PLG24_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24341.1"
                     /translation="MIRVDDSYVENLKVQGLRKPRLNTLYSMSRIFREVYRIPAYIPV
                     SYVCFLEHGVNFQFEHFYNRLLESHGEIIFLDNSHRVANYEARTGKKAYALGPLYPKY
                     RKAHDIRPLGTASGTLAFPSHSSSEFDFSSGYSRYAQDLLQLPERFHPITVCVYYYDL
                     LQGRHKVFEEAGFEVVTNGYIGDEAFVDTFYDTMRRFRYITSNHIGSYSYYALEMGLP
                     FFLYGPDTDKEFIGISDLNGIDMKQYLSAQFYEQFKAAVSIGLPEKPIVTEEQKRLIA
                     IIEDEKNTLPADIVRRMVKKGWIYALVKKLLKKM"
     gene            7877..>8405
                     /locus_tag="PLG24_05315"
     CDS             7877..>8405
                     /locus_tag="PLG24_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HMZ24342.1"
                     /translation="MSQLSKTYNPQDIEGKWYAYWNEKGYFHSEPDNREAYSIVIPPP
                     NVTGILHMGHMLNNTIQDILIRRARLMGKNACWVPGTDHASIATEAKVVKWLREEKGL
                     KKSDLSRDEFMKYAWEWTEKYGGIILNQLQRLGASCDWQRTAFTMDDVRSKGVIKAFV
                     DLYNRGKIYRGLRMIN"
BASE COUNT         2186 a         1931 c         1785 g         2503 t
ORIGIN      
        1 ataaaggtct tggagagatg aatgccgagc agttgtggga gacgacgatg gacccatcca
       61 gaaggactct ccgtcaggtg acaattgaag acgcaatcga atgcgaccgc gtcttctcca
      121 tgctgatggg tgatgaagtt cctcctcgca gggaatttat agaggctaat gccacatatg
      181 ccaagataga cgcctgatct ccggataaat ttgtttcatt tatatataaa aaacgcccca
      241 tctcccgatg aggcgttttt tcttctctga agttgaaagt aattctcagg taaatctgat
      301 ttcatcttcg gctatatcga aaaaacgata ttccgtaagg gcaaaatttg agtttggtct
      361 gatgtttacc catttcttat gcctgaaata ccatttcatc cggatattca gcaagccttt
      421 tgtcaggtag gcgtgtacat atgggtgcac actcaggttg acctttgaaa ttgatttgtt
      481 ggtcaaaaga tagacgaggt ctctttcgat ctcatcagta agcaggatgg acggctggac
      541 ctttccgctt ccattacaag ccgggcaaac ctcatcggta ttgatcttca atgccggtct
      601 aaccctttct ctggtgatct gcatcaaacc aaatttactc aaaggcaaaa tggtgtgttg
      661 tgcccgatcc tgagccatca gttcaaccat tcggttgttg agggctttct tatgatccgg
      721 gttcttcata tcgatgaagt cgatgatgat aaggcctccc aggtctctga gtcgcaactg
      781 cctggttatt tcttcggcag cttcaaggtt gaccgtcagt gatgtgttat tcatctctga
      841 ctttgacatt ttgggtccgc tgttgacatc gatgacatgc atagcttctg tgtgttctat
      901 caccagatat gcaccactgt tcatcgtagc tgttttgccg aaggatgcct tgatctgctt
      961 attgattcca aaatgttcaa aaatcgggag cttaccattg taatgttcta cgatcttgac
     1021 cttttcggga gcaatgctcg acataaacga cttgatgttg ttgaagacct ccttatcatt
     1081 gaccaccaca ctgtgataat catcagacag aaaatcccgg agcaggcttg ttgtcttgtc
     1141 cgcttcgctc aaaacttttt ttgggccttt ggcattggat agattttgtt ctatctcctg
     1201 ccatttctgg gcaagattgc tgatttcatc gtgcaggtca gcgacttttc taccttctgc
     1261 tgcagtcctg gcgatgatac cgaaattttt gggcttgatg ctttctatca ataattgtag
     1321 cctttttctc tcctccggat tgataatctt tttagagatg gtcactgcat tggaaaatgg
     1381 tgtaaggacc agatacctgc cgggaattgt tatctcacaa ctgagacggg gtccctttgt
     1441 cgaaataggt tctttcagga tctgaaccag caacatggat ttcttatcca taacttcctg
     1501 gatctttccg tttttgttga tctcctttga aagatccata ttgtggatat tgaatcccgg
     1561 agtcttgttc accagctgct gtgtacattc aattacagaa ggcagctttg gtcccagatc
     1621 ggtatagtgc aggaaggcat ccttcctctt gccgatatcc aaaaatgcag cattgagtcc
     1681 gggcatcaat ttactgacct ttgccagata aatgtccccg acattgtaag aattactggt
     1741 tttttgcttg tgaatctcca ccaatcggtg gttttccatc aaggcaattt caacttcaga
     1801 aggtaaagaa ttaataatca gctctttatt cacatctgaa agttttagta aaatttcttt
     1861 ttcaaagtat ttaccggcat gccgatttta ttcaacatcc cggtaaatac tgcggttgag
     1921 gtacctaaga tacctttatc gattcttctt cttatgtcga tttttcctta gtctcttttt
     1981 tctcttgtgc gtagcgatct tgtgcctttt acgcttttta ccacatggca taatcgtaga
     2041 tttttttgtt aactaatata ttacttgtgc atgttcttca attgcgacat ttttccgcaa
     2101 gttcatgcgc tttttcatgt tgccctgccc cttcataagc catgctcaac aaacacggcg
     2161 tttgaggatt atcgggatca atcctgtaag actcctccag tctctcgatt gccttatcaa
     2221 attggccggt ctttatcgcc agctcaccaa gaagattaat taccgcagtg ttcccgggct
     2281 cttttttatt gagatccagc aacattttga tgcctttcat cacatcatcc tttggaggag
     2341 catcggcata tatggctgcc agattaagcc ggtggattgt ggcatcaggc tcaatggaaa
     2401 tcgccttttc aaaggccttt gctgcgcgtc ctgcacaaaa ctcccgttca ctcccttcag
     2461 aaagcacctt gacgcccata ccgtaacttg tccccgctat tgaccaggcg gttgccgttt
     2521 tttccaactc agcaactttt tctgcgtaat aaccagccag gccatacctc ctttggctat
     2581 accagatccc ggaaattctc ttcagattgt ccaggatatt tgccgtatcc ttgccttcag
     2641 tagcatgctg caatcgttcc accagttcta acgtgtcccc ggatgctgat aaccgagctt
     2701 ccttcaacag cgcttccggg ccggttgctt ctgtgatcag agagcgttct ttgtcttttc
     2761 tcgcctttgc cttcttgtct gtcaggctga agctaaaata aaggatacaa aaaagtaaaa
     2821 tggcggaacc cagggcgatt ttcttttggc ggctgattcc cgtcggccta ttatttgcca
     2881 tattaatcct cttctacttc cggagcgaca gtcaccctgt ttttcacctg ctctgtaaaa
     2941 gtcttagccg gtttgaaagc aggaatgaaa tgctctggaa tttctattgc ctcattcttt
     3001 ttgatatgac ggcctatttt ttttgctctt ttcttgatga taaatgatcc aaaaccgcgg
     3061 atatatacat tttctccgct ggacaatgtg ttttttactt ccgtaaagaa gtgttctatt
     3121 gacaccagta catccacttt agcgactccg gtcttctcgg caatcgtggc aactaagtca
     3181 gcttttctca ttatttgttg gtttataata agttatgaaa taaaaattca ggctcttata
     3241 aagcgtggca aatttcgtat ttttttcatt ttaaaaaaat ttctgactac tttttttcat
     3301 aatttgttga aattcacaga gtaggataaa atcacatacg tcgcacaggc cgcgcaatac
     3361 aggcaattaa tggaccttta atttttgtca aactttttta tagaagcggc atgtagctcg
     3421 ttgactttcc tggcaatatc ctcgattttt tcctccatca tggtcctgaa tgtgtgcttt
     3481 acatcagaaa atccaatgtg tgcatgatat tgccatatct cctgaatgtc ttcatatgca
     3541 atggtatagg gcataaaacg ctcattgtcc gatattgcta tcagccgggc ctcctcgtcc
     3601 gccttcctga ctcttttata tactacgcca tccttgtggg tgatgatgat ataggttttg
     3661 tcgtccctga tatccctgag gctttctaca aaacgacata tgattataga gcccgactcc
     3721 atcggtaaca tgctgtctcc tgaaatttca aatgcacgat aacgaccgcc ctgcaacgat
     3781 ggaaccgata tcctcggcaa atccgagata tattccggat ctgccatgcc agacatataa
     3841 ccggcctctg ccctggtctc caccaactct atctgatttc tcccctctgt atccaccgtc
     3901 atagccaata cgcgcagctc tttgttcctg aatacttcaa gattatccat cgtcagatcg
     3961 gccttgacca gttgatccaa agtcacatcc aacgcttcac acatcctgcc gagcattttc
     4021 agattaggct ctgtatttcc cttctcgtat tcggaatagg ttgtccgcgg tatgccgagc
     4081 acatcggcaa aattttgctg ggacatctgt ttcttccgtc gaaggtgtct caagttgata
     4141 tgcaacattc tttggggctt ttgtcgccaa aggtatgtat ttttttacac aatgacaaat
     4201 attacggcat tgttgacgaa aaatgcggca tctgctttta aataattctt ctattcaaaa
     4261 agggcgacaa tatcattgtc aaatatccgg aaatttttgc ccgtccggcc tcctcctctt
     4321 tatccatcaa tagctataga cctttccacc acaggcttca acatatgcct ctccatataa
     4381 tgagaatgtc atatccgcct gtcttgccaa agatctgtcc tgctacaata ttgattgctc
     4441 tccgataaaa tccttcttat ttaatgttga attgcattaa atggtctaaa tatccctttc
     4501 tttttcataa atgaattata tccctctttg gggtgtccga aacggctatt gtgatttaat
     4561 catattatta tccttgtgtt tgcgttacct ttgggcaaat tttaccatca atctgtaaca
     4621 gggaaatgaa taacaaggat ttactgaaga tagctgaaca atttgggaca cctacctatg
     4681 tgtatgatgc cgaatccatc aaggcacagt atgataagct ggtgggggcg ttcagcacag
     4741 atacacgatt tttctttgcc tgcaaagcat tgtccaatat caatatcctt aaatatatcc
     4801 gaagcctggg agccggcctc gattgtgttt cgatcaacga agttatgctg gggctgaggg
     4861 ctggctttgg gccggagaag atactgttta ctcccaattg tgtcgacctg gctgagatcg
     4921 aagaggcgat ttgccacaag gtacatatca atatagacaa catctccatc ctggaacaat
     4981 tcggaaataa gtatggcggc tcttatccga tatttatccg tatcaatccg catatttttg
     5041 cgggaggcaa ctataagatt tcaaccggac atattgacag caaattcggt atctctatcc
     5101 accagctgag acatattgaa cgacttgtca aatcgacagg agtcaatatc gaaggcctcc
     5161 atatgcacac gggaagcgag atcaaggacg ccgatgtctt catagaaggg ctggaaatca
     5221 tgtttgaact ggcggaacat tttcctaatc tcaaatacct cgatatggga agtggcttta
     5281 aggtggcata ccgggatggt gatatggaaa ccgatgtggc tgagctgggc caaaaagtag
     5341 ctgcggcaat ggatcggttt gcctcggata cgggaaagaa gcccgaacta tggtttgaac
     5401 ccggcaagtt cctggtgagt aaaagcggcc acttcctggt caggaccaat gtcatcaaac
     5461 acaccacggc aactgtattc gccgggatca attcaggctt caaccacctt atacggccga
     5521 tgttctacga cgcctaccac gaaatccaga acctgtccaa tccaagtggt ccggaaagaa
     5581 tctataccgt agtaggaaat atctgtgaga cggatacctt tgcctgggac cgtaaactga
     5641 acgaaaccag agaaggtgat atccttgtat tccgaaattc tggtgcttac ggcttcgaga
     5701 tgagctccaa cttcaactcg agattgaagc cggcagaagt gctgtttttg gacggcaaga
     5761 ttcatctgat ccgaagaaga gatgcgtttg aagatctgct gaagaatcag attgaagtgc
     5821 tttagatact gatcaaatac tgtttggtta aatttcgatc tatataagaa ttgagacgga
     5881 tggcaagaca acacaatcac ctgaggggca aaaccgacaa tcggaatacg taaccaaaga
     5941 gaaagagatc tgtttttcaa aacgtattaa ctatctttgg ccgatgcagc aagatttaca
     6001 cctcgcagga ctgattataa gcgaaccgac aacatttctg acaaatctgt tgcttgctgt
     6061 taccggaatt tatttaggat ctcgtttttt atccggacgg catgccgggc cggaattcat
     6121 ccggcaatac ggattattct tgatgcttac cggaatatct gccttatggg gcggggtctt
     6181 cagccatgga ctcaaggtct atttcgcggt tgaatattca attccgggct ggattactgc
     6241 ttttgtagct gcttattata tggccggagg ggcaatttcc cacctccaat attgcaacct
     6301 ggcgcctttt gataatgata tcgtcgtccg acgtattaaa atggtaaact tgctaatctt
     6361 gttatcagca ataatattca ttttcataaa gcctcatttt atagtagtgg ctgtcaatgc
     6421 agccatagca ttggttgcgg caggcttgtt ttgcgaatgg attgcctaca aaaacaccgg
     6481 cgacaaaggc agctttttgt ttttggtggc ggtgactatc agtattttga ccattttgtt
     6541 attcctattc aagatcaacg ctgagccttg gttcaacacc aatgacctgt cacaccttgt
     6601 catgattgga tgtatgttta tttttgcaaa agggtttgaa cagatgaaaa taaatatagt
     6661 cagcgcatga tccgggtcga tgatagttat gtcgaaaacc taaaagtaca gggcctcaga
     6721 aagccccgat taaataccct ttacagtatg tcgcgcatct tcagggaggt ttaccggatt
     6781 ccggcttata ttcccgtgtc atacgtctgt tttctggagc atggtgttaa ttttcagttt
     6841 gaacatttct acaacagact tttggagagt catggggaaa tcatctttct ggacaattca
     6901 caccgggtgg ccaattatga ggcccggacc ggcaagaagg cctatgcgct ggggccactt
     6961 taccctaaat acagaaaggc acacgatatc cggccactcg ggacagcttc gggaaccctg
     7021 gccttccctt ctcattcgtc gagtgagttt gacttcagct ccggatatag tcgttatgca
     7081 caggacctgc tgcaacttcc ggaacgattt catcccatca ccgtgtgtgt gtattattat
     7141 gacttgttgc aaggacgaca caaggtattt gaagaggcag gctttgaagt tgttaccaat
     7201 ggatatatcg gcgatgaggc ttttgtggac accttttatg acactatgag gcgattccgg
     7261 tacatcacaa gcaaccatat cggctcctac tcctattatg ccctggaaat gggtctgccc
     7321 ttcttcctat acggaccgga taccgacaag gaatttatcg gtatttctga cctcaacggc
     7381 atcgatatga agcaatacct cagcgcccaa ttttatgaac agttcaaggc tgccgtatcc
     7441 atcggccttc cggaaaaacc tattgttaca gaagaacaaa aaaggctcat agccatcatt
     7501 gaagatgaaa aaaacacact ccccgcagac attgtccggc gaatggtaaa gaagggctgg
     7561 atatatgcac tggtaaagaa gttgttgaaa aaaatgtaaa cacttcgtgg tttttcaagg
     7621 aaggtatagg ttcggaaaaa cacttcaatc ctgtaccgtg atagacacca actcacaaag
     7681 acaaggcgcg tggattgatc caaagacttg ttgtcaaaaa ccaactttcc gccaatccgg
     7741 aggaggtgcg attcagcatt taacggtagt tcatttatca atatcccggc aaccgagtaa
     7801 tccagccatg aatacccggg tcgagttttt ataaaattct cctaactttg cggcttaaaa
     7861 taatttccgg aacaacatgt cacaactgag caaaacgtat aacccacagg atattgaagg
     7921 aaagtggtac gcctattgga acgagaaagg ctattttcac tccgagcctg acaaccgaga
     7981 agcatattcc atagtcatac cgccgcccaa tgtaaccggc atcctgcaca tggggcacat
     8041 gctcaacaac accatccagg acatcctgat tcgacgtgcc cgactgatgg gcaaaaatgc
     8101 gtgttgggtg ccgggtaccg accatgcatc catcgccacg gaagccaagg tggtcaaatg
     8161 gctgcgggaa gagaagggcc tgaaaaaatc agacctcagt cgtgacgaat ttatgaaata
     8221 tgcctgggaa tggacagaga aatacggcgg catcatcctc aaccagctgc aacgtctcgg
     8281 tgcctcctgt gattggcaac gtacagcctt caccatggat gatgtccgca gcaagggcgt
     8341 tatcaaggcc tttgtcgatc tgtacaatcg tggtaagata tatcgcggcc tccgcatgat
     8401 caatt
//
LOCUS       DAOQQN010000102         8395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3284_length_8395_cov_6.199400, whole genome shotgun sequence.
ACCESSION   DAOQQN010000102 DAOQQN010000000
VERSION     DAOQQN010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8395
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3284_length_8395_cov_6.199400"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="PLG24_05320"
     CDS             complement(<1..1050)
                     /locus_tag="PLG24_05320"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HMZ24343.1"
                     /translation="MITSISQIYSQGFLHFKPNNEALSPVLRQKMPNTKVVNVDIRKL
                     YNSIVDNRQVLTIEAESSNWTFRLEENSLISPDYFTTIAGASGNVVSRDISTKTYFGV
                     AEGRSASISLTIDHDFINAMIVDGTKTLFIEQEWIMDHTADRSSVLLYDASEVKPDGS
                     FSCGVTALHEHKNDYHEMLEARTGQCKQVELAIATDASMYTKFSNSVTNVENHNISVM
                     NNVAFNYRHEFVENIEFTIVTQYISTSYANDPLTPNTASTNANTVLPRFSTWSYNGGF
                     GVAHDLGQFWTNRDFDGSTVGLAYVGTVGVTGYKYHILQDFTSDPTSLRVMTAHEMGH
                     NFGAGHDNSGDPYIMA"
     gene            1657..2913
                     /locus_tag="PLG24_05325"
     CDS             1657..2913
                     /locus_tag="PLG24_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ24344.1"
                     /translation="MDLFERLKASDKGHLGRYSEEADGYYMFPKLEGELGPRMTFNGK
                     EVINWSINNYLGLANHPEVRAVDAQAAKDWGLAYPMGARMMSGNTRLHEQLESELAEF
                     VHKESSILLNYGYQGVVSAIDCLVSRHDVIVYDSESHACIVDGVRLHLGKRFAFDHNN
                     MESLRTNLQRAKNIIDETGGSILVISEGVFGMRGDQGKLKEIVAMKKEFPFRLFVDDA
                     HGFGALGKNGEGAGVEQGIQDEIDIYFSTFAKSMASIGAFIASDKFVVDYIRFNIRSQ
                     IFAKSMPMPLVVGNLKRLDMIRRSPEFKNKLWDNVNYLQNGLKSRGFDIGSTNSCVTP
                     VYLKGSIEEAMVLVHDLRENYGIFCSMVVYPVIPKGMIILRLIPTAVHSKEDIDQTLE
                     AFSMIKDRLYSGEYAKIAAERYVAKT"
     gene            2959..4017
                     /locus_tag="PLG24_05330"
     CDS             2959..4017
                     /locus_tag="PLG24_05330"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ24345.1"
                     /translation="MKPYISGIQQIGVGVADVHEAWKWYHRAFGMDIPVFEEAATAAL
                     MLPYTGGKPQDRHAILALNLRGGGGFEIWQYTSRTPQKAAFEVKAGDLGIFIAKIKTS
                     DIKKAFSHLIDCGATMLSEPAQSLNGMRHFYAKDPYDNLFEIIEQKDWFSSGPGVFGG
                     CCGAVIGVSDIEKSKLYYKALLGYETISGETEVMDNEWRNIPGGEKKFKRAILTHEYP
                     RKGPFSKLLGYTSIELIQALDYQPNKIFENRFWGDLGYIHLCFDIQGMDEMRKLCEQF
                     GHPFTVDSSNSFDMGEAAGHFSYIEDPDGTLIEFVETHKIPIIKKIGWYLNLRNRPAD
                     KALPNWMLKALRFSRREL"
     gene            complement(4058..4669)
                     /locus_tag="PLG24_05335"
     CDS             complement(4058..4669)
                     /locus_tag="PLG24_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HMZ24346.1"
                     /translation="MYPLFESIRVENSIIMHPSWHLMRYLDSYTTYYGYEPANGLFDG
                     LIIPEACHAGTFKLRISYNHNSRKATWEPYTIRDITSLRLIEANDIDYSLKYTDRSHL
                     NRLFKQKKDCDDILIIRHGLITDSSYCNIVLFNGEEWHTPAGPLLCGTARQRLLASGQ
                     VIARDISVDDLRSYECFKLINAMRDFETLPSIAVDFIRIDDEC"
     gene            complement(4653..5621)
                     /locus_tag="PLG24_05340"
     CDS             complement(4653..5621)
                     /locus_tag="PLG24_05340"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF005486.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HMZ24347.1"
                     /translation="MTREDFSHKASEWAAAGQPFVFLMDFEMEKPVICKPEDAATHGL
                     HFDIAGISNTDNIPISAIPDLKSSPVSREWYSERFRLVKEHIRHGDSYLLNLTFPTPI
                     DCNLSLSDIYHTARAPYRLLYRDEFVVFSPECFVRIKDDEIFTYPMKGTIDARLEDAE
                     NKLLDNLKEEWEHNTIVDLMRNDLAMVAHDIRVIKYRFVQRIKTYKGEILQTSSEIAG
                     RLKHNWRTRLGEMLTLLLPAGSVSGAPKQKTLDIIRTAELGPRGYYTGVFGYFDGHNL
                     DSAVAIRYIEQGIDGLLFRSGGGITAQSNEEDEYLEMIQKIYVPTL"
     gene            complement(5618..6193)
                     /locus_tag="PLG24_05345"
     CDS             complement(5618..6193)
                     /locus_tag="PLG24_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HMZ24348.1"
                     /translation="MRILLIDNNDSFTYNIVELLRSLDDVLYGVLPSKGLNIAVLEDY
                     SHIVISPGPMTPSDFPELRDVISYCEKLDKPLLGICLGHQAICEYFGGRLVQMDTIVH
                     GHRERIAIDNRSSIYRYLPDRIEVGLYHSWKIDHLNLPDELAVTGMSQEDCLMSVQHN
                     KKQIFGIQFHPESFLTAKGRQILENFVNIGK"
     gene            6678..7682
                     /locus_tag="PLG24_05350"
     CDS             6678..7682
                     /locus_tag="PLG24_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HMZ24349.1"
                     /translation="MRKIFIVILILCITGVVFSQSKQNTMTKADKNNPLLCNPQSGTC
                     EVQDSMVADSVRINISPSVTDKIRVVYFTDPICSSCWGIEPQLKKLKLEYGEVIDVSY
                     HMGGLLPDWSYNSGGISKPSDVASHWDEVSLHYDMPIDGDVWLEDPLSSSYPPSIAFK
                     AAQLQDKDKALDFLRIIREMVFLEKKNITRLQYMSEAASMAGLDTVRFKNDIEGRAVS
                     LFEEDLQLARTYGVRGFPTIFLEGKGGAREMVYSARPYAFYEVAVLKLAPNVVKQEYS
                     KDWQFLFSKYSSLTVKEFAELSGMRRSECEAFLNNLVADGHLTRLTTKNGSIWRRTAK
                     "
     gene            complement(7761..>8395)
                     /locus_tag="PLG24_05355"
     CDS             complement(7761..>8395)
                     /locus_tag="PLG24_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ24350.1"
                     /translation="FNNNIQNYIYMATVADAQGFPITDAQGNRTYQYQQSSAHLFGSE
                     LWFAVHPERLVGFRWDNSLSIVYGLNKNPEFKGKGNEGEFLPLMPPLKVTSRIEYEVK
                     PHTGRLLSITPGFEMEYNHAQNRYLALNGSESFTPEYTLFNLGLNAEVKYNNSKAIQI
                     ILQLNNMLDKAYRSHLNRLKYFEYYSSSPNGHDGIYDMGRNLSLKIIVPL"
BASE COUNT         2315 a         1697 c         1976 g         2407 t
ORIGIN      
        1 agccatgata tagggatctc cagaattatc atgacccgca ccaaaattat gacccatttc
       61 atgtgccgtc atgacgcgta agcttgtagg atcagaagta aaatcctgaa gaatgtgata
      121 cttatatccg gtgaccccaa ccgttcctac ataagcaagg cctactgtcg agccatcaaa
      181 atcacggttt gtccaaaatt gccccaaatc atgagctact ccgaatccgc cattataaga
      241 ccacgtagag aatcttggca ggactgtatt tgcgttagtt gatgctgtat tgggggtcag
      301 cgggtcattg gcataagatg tgctgatata ttgggtgacg atagtaaatt ctatattctc
      361 aacgaattcg tgtctgtagt tgaaagctac attgttcatc acggaaatat tatggttctc
      421 aacatttgtc acagaattgc tgaatttggt gtacatggag gcatcggtag caatggctag
      481 ttccacttgt ttacattggc cggttctggc ttccaacatc tcatggtagt catttttatg
      541 ttcatgcaac gctgtaacac cacaagagaa ggagccatcc ggcttaacct cactcgcatc
      601 atataagagg acggaactgc gatcggccgt atggtccatt atccactcct gctctataaa
      661 cagggtcttt gttccatcca caatcatcgc attgatgaag tcatgatcga tggttagact
      721 gatggaagcg cttcgtcctt ctgctacacc aaaatatgtt tttgtgctga tgtctctact
      781 gactacattg ccggaagctc ctgctattgt agtaaaataa tcaggagaaa tcaaactgtt
      841 ctcttccagg cggaatgtcc agttactact ttcggcttct atagtaagaa cttgtcggtt
      901 atcgactatg gagttataaa gtttccgtat atcgacattt actaccttag tatttggcat
      961 cttctgacgc agtaccggac ttaatgcctc attattgggt ttgaaatgaa ggaagccctg
     1021 gctgtatatt tgactgatgg acgtgatcaa aaaaatgaaa ataacaataa ttccggttcg
     1081 gtacataggt agtacttttg attgattaaa caaaaatgaa gtgtgtgctt ttgggcggga
     1141 gattgaagtc aaggcgattc aggccggaat gtgctaagag cgtggatcca aacctatgga
     1201 tgccttcatg gttatggccg ggtgagttgc aaatccaatt ggacaaagca atatattaat
     1261 attaatacga atatataact ttctcctgaa aatgcgtata taaaattatg ttattaacaa
     1321 aaaatttgtt gaataataag aataccttga gttctttgat aaaaatataa tcaaaaaact
     1381 tatactttag tttgaatctg aatttgttga tcggcatggg gaagatgtcc atgtataccg
     1441 aggtaaaagg gatattcagt tattgattgg cttccaattt ttagtgaata gcatgtcgcc
     1501 ggagttatta atttgacagg aatcatgtct ttagagtttt atatcccttg aggcaatatt
     1561 tgccctttta tcttcggatc gttcaatacg ttcagatttc ttttcctaat ctccttaatt
     1621 tttatcaact ttgcgcctta caattttaat ttaaaaatgg atttatttga aagattgaaa
     1681 gcttctgaca aaggccacct tggccgatat tcggaagaag ctgacggata ttatatgttt
     1741 cccaagctgg aaggcgaatt aggtccccga atgaccttta acggaaagga agtgatcaac
     1801 tggagtatca acaattatct cggtctggcc aaccacccgg aagtacgcgc cgtagatgct
     1861 caggctgcca aggattgggg cctggcatat ccgatgggtg ctcgtatgat gagcggtaac
     1921 accaggctgc acgagcaatt ggaaagcgag ctggctgaat ttgtccataa ggaatcgtcc
     1981 atcttgctca attatggata tcagggcgta gtttctgcta ttgattgtct tgtaagccga
     2041 cacgatgtca ttgtatatga ttcggaaagc catgcctgta ttgtagatgg tgtcaggttg
     2101 catttgggta agcggtttgc cttcgatcac aacaatatgg aaagcctccg tactaatctc
     2161 caacgtgcca aaaacatcat cgatgagaca ggtggaagca ttttggtgat ttcagaaggt
     2221 gtatttggca tgcgtggaga ccagggtaag ttgaaagaaa tcgtagccat gaagaaggaa
     2281 tttccgtttc gactgtttgt ggatgatgcg catggatttg gtgccctggg caagaatgga
     2341 gagggtgccg gtgttgagca gggaatccag gacgaaatag atatttattt ttccaccttt
     2401 gccaagagta tggccagtat tggcgccttt attgccagtg ataagtttgt ggtggactat
     2461 atccggttca acatcagaag tcagatattc gcgaagtcta tgcccatgcc gcttgtagtg
     2521 ggcaacctga agagacttga catgatccgc cgaagccccg aatttaagaa taagctttgg
     2581 gacaatgtca actacctgca aaatggcctt aaatcaagag gattcgatat cggctccacg
     2641 aatagctgtg tgactcctgt atatttaaag ggcagtatcg aagaggccat ggtattggtt
     2701 catgatctgc gagagaatta cggaatattc tgctccatgg tggtatatcc ggtcatacca
     2761 aagggcatga tcatcctgcg attgataccg actgcagtcc atagcaagga ggatatcgat
     2821 cagacgctgg aagcattcag tatgatcaag gataggttgt atagcggtga atatgcaaag
     2881 attgccgctg agcgatacgt agcaaaaacc tgatgaagac agtataattc cattaccatt
     2941 ctaacagaat attatatttt gaagccttat atcagcggaa tacagcaaat aggagtcggg
     3001 gtagccgatg tccatgaagc ctggaaatgg taccatcgag cattcggaat ggatatcccg
     3061 gtgtttgaag aagcggcgac tgcggcattg atgctgcctt ataccggagg taagccgcag
     3121 gacagacatg ctatcctggc cttgaatctc cgcggtggcg gtggttttga aatatggcaa
     3181 tacaccagca ggacacctca gaaagcagct ttcgaagtga aagccggtga cctgggtatc
     3241 tttattgcca agatcaagac ttctgatatc aaaaaggcat tcagccacct gattgattgt
     3301 ggggcaacaa tgctgtctga accggcgcaa tccctcaatg gcatgcgaca tttctatgcc
     3361 aaagatccat atgacaacct gtttgaaatc atcgaacaaa aggattggtt ctcttcgggg
     3421 cctggagtct ttggggggtg ttgcggggca gtgattggtg tttccgacat tgaaaagtcc
     3481 aaactttact ataaggcctt gttgggttat gaaacgatct ccggagagac tgaagtaatg
     3541 gacaatgaat ggcggaatat ccccggtggt gaaaagaaat tcaaaagagc tatccttaca
     3601 cacgaatacc cgcgaaaagg gcctttttcc aagctattgg gctatacttc catagagctg
     3661 atccaggcgt tggattatca accgaacaag atctttgaaa accgattttg gggcgacctg
     3721 ggatatatcc acctttgctt tgatatccaa ggtatggatg agatgagaaa actgtgcgaa
     3781 cagtttggcc atccattcac cgtggacagt agcaatagtt ttgatatggg agaggccgca
     3841 ggtcattttt cttacatcga ggacccggat ggcacactga tagaatttgt cgaaacccac
     3901 aaaataccga tcatcaagaa gataggctgg tatcttaacc taagaaacag acccgcagac
     3961 aaagcattgc ccaattggat gcttaaggcg ctgcgatttt cgagaaggga gttgtaagag
     4021 ggttggttcc ggcatgtagt tactatatcc tgttagttta gcattcatca tcaatccgga
     4081 tgaaatctac ggctattgaa ggcagagttt caaaatcgcg catggcattg atgagtttga
     4141 aacattcgta actacgaagg tcatcgacac ttatatcccg ggctatgact tgcccagaag
     4201 caagcaggcg ttgccgggcg gtcccgcaaa gtaaagggcc ggcaggggta tgccactctt
     4261 ctccattgaa caacactatg ttgcagtaag atgaatctgt gatcaagccg tggcgtatga
     4321 ttaggatgtc atcacagtct ttcttttgtt tgaataacct gttcaggtgg cttctatccg
     4381 tgtatttcaa ggagtagtcg atatcattgg cttcgatgag gcgcagggaa gtaatgtccc
     4441 tgatggtgta tggttcccat gtggccttgc gggagttatg attatagctg attcgcagct
     4501 tgaaggtgcc tgcatgacaa gcttccggga taatcaggcc atcaaaaagc ccgttggccg
     4561 gctcatatcc gtagtaggtt gtgtacgagt cgagatatcg catcaggtgc catgacggat
     4621 gcatgatgat gctgttttcg acccggatgg attcaaagag tgggtacata gattttctga
     4681 atcatttcaa ggtattcatc ttcttcgttg ctctgtgccg ttatgcctcc accacttcta
     4741 aacaacaagc catcgattcc ctgttcgata tatcggatcg ccacggcact gtccaggtta
     4801 tgtccatcaa aatagccgaa gacgcccgta tagtaaccgc ggggacccaa ttcggctgtc
     4861 cggattatat ccagggtttt ttgcttgggt gcaccgctga cggaacctgc aggaagaagg
     4921 agggtcaaca tttccccaag ccttgtacgc caattgtgtt tcaaccgacc ggctatttcg
     4981 gaactggtct gaagaatctc gcctttgtac gtcttgattc tttggacaaa acggtatttt
     5041 atcacccgga tatcatgcgc gaccatagcc agatcatttc gcatcagatc cacgatggtg
     5101 ttgtgctccc attcttcttt aagattgtcg agcaatttgt tttctgcatc ttctaatctc
     5161 gcatcgatgg tgcctttcat cggataggta aatatttcat cgtcttttat cctgacaaaa
     5221 cattcaggag aaaaaaccac gaattcatcc cgatagagca accgataagg cgcacgggca
     5281 gtatggtaaa tatctgacaa gctgagattg caatctatgg gtgttggaaa tgttaggttg
     5341 aggaggtagg aatccccgtg tcggatatgt tccttgacca accggaatcg ctctgagtac
     5401 cattccctgc taacagggct gcttttcaga tcgggaattg ctgaaatggg aatattatcc
     5461 gtgttgctga tgccggcgat atcgaagtgt aatccatgag ttgctgcatc ttcgggtttg
     5521 cagataacag gcttttccat ctcaaaatcc atgaggaaga caaatggctg tccggcagca
     5581 gcccattcag aagccttgtg cgaaaaatcc tctcttgtca ttttcctata tttacaaagt
     5641 tctccaggat ttgccttcct ttagcggtca gaaaggattc agggtggaat tgtataccga
     5701 aaatctgctt tttattgtgc tggacagaca tcaggcaatc ctcctgggac atgcccgtta
     5761 ctgccaactc atccggcaaa ttaagatgat ctatcttcca ggaatgataa agacccacct
     5821 ctatcctgtc cggcaaatac cggtagatgg atgaacgatt gtctatcgca attctttccc
     5881 tgtggccatg tacgatcgta tccatctgca caagtctgcc tccaaaatat tcacagatgg
     5941 cctgatggcc gagacagata ccgagcaacg gtttatcaag tttttcacaa taagaaatga
     6001 catctctaag ctcaggaaag tccgaaggag tcattgggcc gggagatatg acgatgtggc
     6061 tgtaatcttc caatacagca atgttgagac cttttgaagg caaaacccca tataacacat
     6121 catcaagact gcgcaataac tctacaatgt tgtaggtaaa ggaatcgttg ttatctatca
     6181 acagaatacg catcttaagt ttgccattgc tatttagaag ggcaaaaata atctaaataa
     6241 cattcaggca aatattcatt tcgcgttgag ggggaatgaa tatcaattcg tctttgcata
     6301 cagacgcgat gagccttcat cgaccgtgtc aggtcaatga tacagggaat tactaataca
     6361 ttgttgggca ttttataata ttggaccgtg tacaaaattt ttactttaat gagaagggaa
     6421 gtaaaaggga taatttcggc ttgatcttta gtctgccttg ccattaacat gttggaaaag
     6481 cagtatctat acatgagggt ctgacggata agaatggatc tcttccttaa catacattca
     6541 atgaacattg tgcccgggtt gagttgaaat atggatttac aggtttatta taagcacgat
     6601 ggtggattca tttttgcctg attgcataac ttttcctttc ggtcgttgtt ttaacccgaa
     6661 acaaatccat tgtattggtg cgtaagattt tcattgttat acttatcctg tgcatcacag
     6721 gtgttgtttt tagccaatcc aaacaaaata ccatgacaaa ggctgacaaa aataatccat
     6781 tattgtgcaa tccgcagtcc gggacttgtg aggtgcagga cagtatggtt gcagattctg
     6841 tccggattaa tatctcgccg tccgttaccg acaagataag ggtggtttat tttaccgatc
     6901 ctatttgttc ttcttgctgg ggcattgaac cacagcttaa gaaactgaag ctcgagtatg
     6961 gcgaggttat cgatgtgtct tatcacatgg gtggtctgtt gccggactgg agttacaaca
     7021 gtggcggaat aagcaaacct tcggatgttg caagccattg ggatgaagtg agtcttcatt
     7081 atgatatgcc gatcgatggt gatgtttggc tggaggatcc attgtcgtca tcctatccgc
     7141 cttccattgc tttcaaggcg gcccagctgc aagacaagga taaggctttg gattttctcc
     7201 ggataatcag agaaatggtc tttctggaaa agaaaaacat aacccggcta caatatatgt
     7261 ccgaggcggc atcgatggcc ggattggata cggtacgatt taagaatgac atcgaaggga
     7321 gagcggtgag cttgtttgaa gaagatctgc agttggcccg aacctatgga gtgagaggat
     7381 ttccgaccat ctttctggaa ggcaagggtg gcgcgaggga aatggtatat agtgccaggc
     7441 cctacgcctt ttacgaggtg gcagtgctga agctggctcc caatgtagtc aagcaggagt
     7501 atagcaaaga ttggcagttt ttattttcca aatattcttc acttaccgtt aaagagtttg
     7561 ctgagttgtc aggcatgcgg cgctccgaat gtgaggcatt tctcaacaat ctggttgccg
     7621 atggacactt gaccagacta accaccaaaa acgggtccat ctggcggcgc acagcgaaat
     7681 gatttaaaca caggattgta ggctgagtcc gttgtagcga gaatgtttcc cggatttgat
     7741 gaattttcaa actattgcgt ttataggggc actattattt tcaggcttag attccgtccc
     7801 atatcatata taccatcatg tccattaggc gaagaactat aatattcgaa atactttaag
     7861 cggttcaaat gtgatcggta ggctttgtcc agcatattat tcaattgcaa gataatttga
     7921 atggctttcg aattattgta tttaacttct gcatttaagc ccagattgaa aagcgtatat
     7981 tccggtgtaa aagattcgga tccattcagc gctaaatacc gattttgggc gtggttatat
     8041 tccatttcaa atcccggcgt aatcgaaaga agtcttccgg tgtgaggttt tacttcgtat
     8101 tcaatcctgc tcgttacctt taaagggggc attaatggta aaaattcacc ttcattgcct
     8161 ttgcctttaa attccggatt tttatttaat ccgtatacaa tactcaaact attgtcccac
     8221 cgaaatccaa caagcctttc cgggtgaaca gcaaaccata gctcagatcc aaataagtgt
     8281 gcagaagatt gttgatattg gtacgttctg ttgccttgcg catcagtaat cgggaagccc
     8341 tgtgcatcag ctacggttgc catatagata taattttgaa tgttgttatt gaaga
//
LOCUS       DAOQQN010000103         8388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3289_length_8388_cov_4.418697, whole genome shotgun sequence.
ACCESSION   DAOQQN010000103 DAOQQN010000000
VERSION     DAOQQN010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8388
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3289_length_8388_cov_4.418697"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..3635
                     /gene="mfd"
                     /locus_tag="PLG24_05360"
     CDS             264..3635
                     /gene="mfd"
                     /locus_tag="PLG24_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HMZ24351.1"
                     /translation="METIEEIIDRFGSSNPVWPQGDSVKNYYIEGLSGSGPLIFMTSA
                     YRKNPRPFLFVADDKDRAAYLYDTLLTFKPGPVHLLTDSFRRPGRFDEVNAANVLRKS
                     EVSSAISSSVTPLIVVTYPEAIFEKIIKPAELEKDKITVTVGELFDQDFVMNILIEYG
                     FERVDFVYEPGQFSLRGAIVDIFSFGNSQPYRIEMDDDKVESIRVFDPNTQLSVKNVN
                     HLNILPNANVKFESEDRIGLLDLMDTRWHIVLEDRNVLLDKIKAAYEKAEDSLHNASL
                     IEQPEAQKFLRDRAFISPDIFEESLKKYTIFYLSKPDGRIKYETLHFQTKPHPVINKQ
                     FKLLKEHLEENAEKGIKTWFFSENKRQLQRLEAILNDLGAPNTFQPVIGVVHEGLTDE
                     KSKVEILTDHQLFQRYHQYSLKTGYTRDQAVTLRMLKELQPGDYVTHIDHGVGKFSGL
                     ETIDIGGVKQEAVRILYRNNDILYVSINSLHKLSKYVGKDGEPPMINKLGSDSWKQLK
                     QKTKRKVKDIAEGLIKLYAKRKASDGFAFAPDGYLQAELEASFLYEDTPDQFKATQEV
                     KEDMEKPYPMDRLICGDVGFGKTEIAIRAAFKAINDGKQVAVLVPTTILALQHFHTFN
                     NRLRDFSVDVQYLNRFKSTKEKKEVYESLKSGKTDLVIGTHALLNKDVAFKDLGLLII
                     DEEQKFGVAAKEKIRSMMVNVDTLTLTATPIPRTLQFSLMAARDLTVIRTPPLNRQPI
                     YTEIKPFEQRAIREAIENEMRRGGQVFFVHDRVKNLGEMVEFILKLVPDANVAAAHGQ
                     MDSKLLEQTLVDFIEGKFDVLVSTNIIETGLDIPNANTMIINNAHHYGLSDLHQLRGR
                     VGRSNKKAYCYLIAPPMSSLTNEAKKRLKTIVEFSELGSGFEIAMRDLDIRGSGNLLG
                     GEQSGFISEIGYDAFQKILEEAIRELKEEEFKETFREEINTKKVHVRDVVIETDGEML
                     IPNNYVTSTSERFALYHEMDAIEDEEALQKFLLDMADRFGPIPDAVKELADGLRMRWT
                     MRVLGFERGVIKGGHLSLFFPSNPQASYYESETFKAIFQMLATTKNPNLSMQKTLRNL
                     IFKIKGIRSVQDSLHFLTELDGQIDSNRQTMPKSN"
     gene            complement(3652..5346)
                     /locus_tag="PLG24_05365"
     CDS             complement(3652..5346)
                     /locus_tag="PLG24_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMZ24352.1"
                     /translation="MANLFRKKSIQTILADSAAGMEDGHGFGLKKVLGVKDLTLLGVA
                     AVIGAGIFSSIGKAAYDGGPGVVYLYIFTAIACGFAALCYAEFASTVPVSGSAYTYSY
                     VAFGELFAWIIGWDLLMEYAIGNIAVAISWSDYFTNLVGKAGLHIPDWLTMDYVTAHA
                     GFEKVNAALGTGANISSMDVVELSKYYAWTNAPSIGPLRFIIDLPAMAIVALITYIVY
                     VGIRESRTASNIMVGIKLAIILLVVLIGAFYVNTDNWSPFTPNGIGGILKGVSAVFFA
                     YIGFDAISTTAEECKDPQRDLPRGMIYALIICTVLYVAVSLVLTGMVHYSRLNVGDPL
                     AMVFDAVDMKFVSAIVAGSAIIATASVLLVFQLGQPRIWMSMSRDGLLPKVFSRIHPK
                     YGTPSFSTILTGFVVAVPALFLNLDVVLALTSIGTLFAFVLVCGGILVLQQNPDRPKS
                     KFRVPYINGQWIFPIGLVAAIVAILYNYPTYFHDLLTWENFPMIVFWVVIPVLAFYTI
                     RRKFSLIPILGLVSCFYLMAQESHTNWFRFIIWLVLGLVVYFLYGFTHSKLAKEGK"
     gene            complement(5608..7536)
                     /locus_tag="PLG24_05370"
     CDS             complement(5608..7536)
                     /locus_tag="PLG24_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ24353.1"
                     /translation="MKRLMIFLVIGGLIQSCAIAQPLVTLKDASDKTKKVFDEGRKAA
                     MAQSFGTAKAKYREALQLTPGFQDAMMELAGIYFSQKNMDSSIFYLNQILTVNPKPSA
                     RVYFTLGTIYYDRYEYREAIPLFEKYLQSDVKNPKSVEDAKIKLANSQFAIEALKNPR
                     SFEPKPVPGEINTELPEYLPSESADGTVLVFSRMIDGQEDLYFSSYDGNSWSRAVPLD
                     AINTEHYNEAGQCISADGHTIVYTGCNMPGGLGSCDLYISYLENGKWTRPKNMGAPMN
                     TPGWESQPSLSADGSTLYFASDRLGGYGLKDIWFSERSPDGVWSTPKNIGAPVNTLKN
                     DGSPFIHPDNETLYFMSDGLPGMGGTDIYLSRRQEDGSWGTPVNLGYPINTSGNEGAL
                     VVSPAGDIAYYTAIKDGGKRSKEGFDDTDIYYFQLDSAIRPKPVSFFKARMLDAMAGK
                     PVRGLVQVTDIRTDSLYYKGLAADDGTVLVCLPKGGLYGIQIIARGYLFVSESVEIPD
                     GGDADHPFIAEYLLHRIEESKGKAMVLKNIYFTSGSAELSPVSDAELKVLAAMFFANP
                     DIRVRINGHTDDVGSDEDNIQLSVMRANSVMERLIELGVSAKSLSAKGFGESVPIGDN
                     TTEAGRKSNRRIEFEILP"
     gene            complement(7533..8270)
                     /locus_tag="PLG24_05375"
     CDS             complement(7533..8270)
                     /locus_tag="PLG24_05375"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HMZ24354.1"
                     /translation="MLKRQDTHIVAVHQPNFFPWFGYFVKIALSDTFILLDDVQIPWR
                     GYTRRTKIRRPGNDPLLEWCPVIIPSGYTYAGINKIPLDDRRQWQGELSGRLESRYRS
                     GAGFKELFPRVQAWLNNGATNLATYNTAIIRNIMDLMDIRKELNVSSDYPVSSSGSAR
                     IVELMQYFGGNVYLSGRGGNKYHRENDFIEAGIKIIEVPNLLESPLLMEVEGINPEAS
                     ILEWLFMLGIDRTREFFYKYLDTIVIG"
BASE COUNT         2554 a         1991 c         1719 g         2124 t
ORIGIN      
        1 atacatcaga aaaaaaatta aatagaattt tgctcaaatc aagtgtaaga ttaaataatt
       61 gctcccaaac gaatcttaat tgcaaatttt gtcatgtatt gaaatagcaa aaacctcttt
      121 aaagccaata aatccaggtt aaatgcagca ggcaacggca tacatatatt cgcacattaa
      181 aaatctgccc taagaattcc acagttaaaa tcttgagctc aacttagaga acgataagca
      241 gtcatttttt ttacatttgc actgtggaaa ccatagaaga aattattgac aggtttggat
      301 catccaatcc ggtatggccg caaggcgaca gtgttaaaaa ttattatatt gaaggattga
      361 gcgggagtgg gccgctaata ttcatgacat cagcatatcg aaaaaaccct cgcccattcc
      421 tgtttgtcgc tgatgataaa gaccgcgcag cctatcttta cgatacctta ctgaccttca
      481 agcccggacc ggtgcatctt ttgactgatt cattcagacg accgggtcgc tttgatgaag
      541 tcaacgcagc taacgtatta cgtaaatccg aggtttcttc agccatctcg tcttccgtta
      601 ctcccttgat cgttgttacc tatccggaag caatctttga aaagattatc aagcctgctg
      661 aacttgaaaa agacaagata acggtcactg taggtgagct tttcgaccag gattttgtga
      721 tgaacatcct gatagaatat ggattcgaac gtgttgactt tgtatatgaa ccgggtcagt
      781 tctctctgcg tggagcgatt gtggacatat tttcctttgg caacagccaa ccatacagga
      841 tagaaatgga tgatgataag gtggagagca tcagggtatt tgatcccaac acacaattgt
      901 ctgttaaaaa tgtcaatcat ctcaacattc tgcccaatgc caatgtcaaa ttcgaatccg
      961 aagacagaat tggcttactg gacctgatgg acacaagatg gcatattgtg ttggaagatc
     1021 gtaatgtatt gcttgacaaa ataaaggcgg catacgaaaa ggcggaagat tcactacata
     1081 acgcatctct gatagagcaa ccggaagcac aaaaatttct gcgggacagg gcttttatct
     1141 ctccggatat ttttgaagag agcttgaaaa aatataccat tttctaccta tcaaaacctg
     1201 atggaagaat caagtatgag acccttcatt ttcagaccaa gccacatccc gttatcaaca
     1261 aacaattcaa attactgaag gaacatctgg aggaaaatgc cgaaaaagga atcaaaacct
     1321 ggtttttcag tgaaaataaa cggcaactcc agcgcctgga agccatcctc aatgacctgg
     1381 gagcaccaaa taccttccag cctgtgatag gtgtcgtgca tgaaggtctg acggacgaaa
     1441 aaagcaaagt tgaaatactc actgaccatc agctatttca acgttatcat caatacagcc
     1501 taaaaaccgg ctatacgcgt gatcaggctg ttactctgag gatgttaaag gaattgcagc
     1561 ccggagatta tgtcacccac atcgatcatg gtgtaggcaa attctccgga ctggaaacca
     1621 tcgatattgg tggtgtaaaa caggaagctg tacggatact gtaccgcaac aatgacatcc
     1681 tgtatgtcag tatcaactct ctgcataagc tttccaagta cgtcggtaag gatggagagc
     1741 cgcccatgat caacaaattg ggttctgact cctggaagca actcaaacaa aagactaagc
     1801 gcaaggttaa ggatatcgcc gagggactca tcaaattata cgccaagcga aaagccagcg
     1861 atggatttgc cttcgctcct gatggatatc tacaggcgga gttggaagcg tcttttctgt
     1921 atgaagatac tccggatcaa ttcaaggcta cgcaggaagt caaggaggat atggagaaac
     1981 cttatcctat ggatcgactg atctgtggag acgttggttt tggaaaaaca gaaatagcca
     2041 ttcgtgccgc attcaaagcc atcaatgacg gcaagcaagt agcagtgctg gtaccgacca
     2101 ccattcttgc actacagcat tttcatacat ttaacaacag actcagggat tttagtgtcg
     2161 atgttcaata cctcaaccgt ttcaaatcaa ctaaggaaaa aaaagaggta tatgaaagtc
     2221 tgaaatccgg gaaaactgac ctcgtcatag gcacacatgc tttattgaac aaggatgtag
     2281 cgtttaaaga cctcggctta ctgataattg atgaggagca aaaatttgga gtggctgcaa
     2341 aagaaaaaat tcgatccatg atggttaatg tcgatacgct caccttgacc gccacaccca
     2401 taccccgtac acttcaattt tccctaatgg ccgcacggga cctaaccgtc atacgtacac
     2461 ctccattaaa cagacaacca atatacacag aaataaagcc tttcgaacaa cgagccatcc
     2521 gggaggctat cgaaaacgag atgagacgtg gcggccaggt gttctttgtc catgaccggg
     2581 tgaaaaatct gggggaaatg gtggaattta tactgaaatt agttccggat gccaatgtcg
     2641 ctgcagcaca tgggcagatg gacagcaaac ttctggaaca aactcttgtc gattttattg
     2701 aaggaaagtt cgatgtcctg gtatcgacga atatcatcga aaccggactg gatattccaa
     2761 acgccaatac gatgatcatc aacaatgcgc atcattacgg cttgagcgac ctccaccagc
     2821 ttaggggaag ggttggtcgc tccaacaaaa aggcatattg ctacctaata gctccaccca
     2881 tgtcctccct taccaatgaa gcaaaaaaac gtctcaaaac catagtcgaa ttctccgaat
     2941 tgggaagtgg attcgaaata gccatgcgcg atcttgatat cagaggcagt ggcaacctct
     3001 taggtggtga acagagtggc ttcatctctg aaatcggtta cgatgccttc cagaagattt
     3061 tggaagaagc gattcgggag ttgaaggaag aagagttcaa agaaaccttc cgggaggaaa
     3121 tcaataccaa gaaagtacat gtccgtgacg ttgtgataga aactgacgga gagatgctca
     3181 taccaaacaa ttacgtcaca tccaccagtg aaagatttgc tttataccac gaaatggatg
     3241 ctatagagga tgaagaagcc ctacagaaat tccttttgga tatggcagac cgcttcggac
     3301 caataccgga tgcagtaaag gagttggccg atggactaag gatgcgctgg acgatgcgtg
     3361 tattgggctt tgagcgcggt gtgataaaag gaggtcattt gagtctgttt tttccatcca
     3421 acccacaggc ttcttattac gagtccgaaa cttttaaggc catcttccag atgctggcaa
     3481 ctactaaaaa tccaaatctt tccatgcaga aaaccctgcg taatctgatc ttcaagataa
     3541 aaggaattcg cagtgtccag gactccttac atttcctgac agaattggac ggtcaaatag
     3601 attccaacag acaaacaatg cctaaatcaa actaaagcga tatccgaatt tttattttcc
     3661 ttctttggcc agcttactgt gggtaaatcc atacagaaaa taaacgacca gtccgagaac
     3721 caaccaaatg ataaacctga accaattggt atgactctcc tgagccatta aataaaaaca
     3781 gcttaccaat ccaagtattg gaatcagcga aaacttacgc ctaatggtgt agaatgcaag
     3841 caccgggatt accacccaaa agacaatcat cggaaaattc tcccatgtta ataagtcatg
     3901 gaaataagta gggtaattat ataagatggc tacaatagct gccaccaatc caattgggaa
     3961 aatccactga ccgttgatat atgggacccg gaacttactc ttcggacgat cggggttctg
     4021 ttgcaagaca aggataccac cgcatacaag gacaaaggca aacaaggtac cgatgcttgt
     4081 caaggcaagt acaacgtcaa gattgaggaa taatgccggc accgccacta caaaaccggt
     4141 caaaatggtc gaaaatgaag gcgtgccgta tttaggatga atacgcgaga aaacctttgg
     4201 caaaaggcca tcccggctca tgctcatcca tattctaggc tggcccaact ggaataccag
     4261 caagacgctc gccgtggcga tgatggcact accggcaacg atagccgaga caaacttcat
     4321 gtccactgca tcaaaaacca tcgccagcgg gtcaccgaca ttcaacctgc tgtaatgcac
     4381 cattccggtc agcaccaatg acactgcaac gtatagtacg gtacagatga tcaaagcata
     4441 gatcatgccc cggggtaagt cccgttgagg atccttacat tcctcggccg tggtactgat
     4501 ggcatcaaaa ccgatataag caaagaaaac agctgacaca cccttcaata tccctccaat
     4561 cccgttcgga gtgaaaggcg accagttatc cgtattgaca taaaaggcac cgatcaggac
     4621 aacgagtaga atgattgcca gtttaatgcc caccatgata ttgctggcag tcctggactc
     4681 cctgattcca acataaacaa tataagtgat cagagcgact atcgccatcg ccggcaagtc
     4741 gatgatgaat ctcagtggtc ctatactagg ggcattggtc catgcataat attttgacaa
     4801 ctctacaaca tccatagagg atatgttggc gccagtaccc agggccgcat tcactttctc
     4861 aaagcccgca tgggctgtca catagtccat cgtcagccaa tccggaatat gaagtccggc
     4921 tttaccgacc aggttggtaa aataatcact ccaacttatg gcaaccgcta tattaccgat
     4981 ggcatattcc atcaacaaat cccaacctat gatccaggca aataactccc caaaggcaac
     5041 ataggaatac gtatatgcac tacctgaaac aggcactgtg gaagcaaact cagcataaca
     5101 caaggcagca aaaccacagg caatagcggt aaaaatatag aggtacacaa cacccggtcc
     5161 gccgtcatag gctgccttac cgatgctcga aaaaattcct gctccgatga ccgcagcaac
     5221 gcctagcaat gtcagatcct tgacgccaag tactttcttg agaccaaatc catgcccatc
     5281 ttccatcccg gcagcagaat ccgccaatat tgtctggatc gatttcttcc tgaataaatt
     5341 cgccatataa cagcgtgttt cgttttttgt tttgtgatgc gggtaaaagt aagaaatatt
     5401 gtcggaaaat tcaaaatctg cccaattatg catggattat tcaccaatat atgcaatttt
     5461 caagggccta tagcgtgtca agcaatgcac cgggctttaa tttgttgatc tgaaaattta
     5521 tccaatattt atatcaaaag aattgtccgt tgtaatattg ttctatggca accatccggc
     5581 ccattgtctc tttcaaaaca gcaaccttta tggcaggatt tcaaattcta tgcgccggtt
     5641 ggattttctt ccggcttcgg tcgtattatc accgatgggc acagactccc cgaagccttt
     5701 ggcagacaat gatttggcag agacacccaa ttcaatcaaa cgttccatga ctgaatttgc
     5761 cctcataacc gacagttgga tattatcctc atcacttcca acatcatcgg tatggccatt
     5821 gattcgtacc cggatatcag gattggcgaa aaacatagct gccagaacct tcaattcagc
     5881 atccgataca ggagaaagct ccgcacttcc acttgtgaag taaatatttt tcaggaccat
     5941 cgccttgccc tttgattctt caatcctatg caacaaatac tctgcgatga aaggatgatc
     6001 agcatcccct ccatccggta tttcaaccga ttctgataca aataggtatc ccctggctat
     6061 gatctgaata ccatacaggc cgcctttggg tagacagacc agcacagtcc cgtcatcagc
     6121 agccaacccc ttgtaataca gagaatctgt cctaatgtca gtcacctgaa ccaggcccct
     6181 gacaggtttg ccggccatgg catccagcat gcgagccttg aaaaaagata ccggcttcgg
     6241 tctgatcgct gaatccaact gaaaataata gatatccgta tcatcaaatc cttcttttga
     6301 tcgcttcccg ccatccttta ttgccgtata ataagcgata tcaccggcag gactgactac
     6361 cagtgcgcct tcattacctg atgtattgat gggatatccg agattgacag gagtacccca
     6421 ggagccatct tcctgcctcc ttgaaagata aatatcggta ccgcccatgc ccggcaaacc
     6481 gtcggacatg aagtataggg tctcattatc aggatgaatg aaaggactgc catcgttttt
     6541 cagcgtattg acaggagcgc caatattttt cggtgtagac cagacaccat ccgggcttct
     6601 ttccgaaaac caaatatctt tcaatccgta tccacctaag cggtcgctcg caaaatacag
     6661 ggtggaccca tctgcactaa ggctgggttg gctttcccat ccgggagtat tcatcggagc
     6721 tcccatgttt tttggccttg tccattttcc attttccaaa taagaaatat acaagtcaca
     6781 gcttcccaac cctccgggca tgttgcaccc tgtatacaca atagtgtgcc catcagcact
     6841 aatgcattga ccggcttcat tataatgctc agtgttgata gcatccaaag gaacagctct
     6901 tgaccaacta ttgccatcat agctcgaaaa atacaaatcc tcctgaccat caatcattct
     6961 ggagaagacc aatacggtac catcagcact ttcagagggc agatactcgg gcaattcggt
     7021 attgatctcg cccggcacgg gtttgggttc aaaagaccga ggattcttca aggcctctat
     7081 tgcaaactga ctattggcaa gctttatctt tgcgtcttca acggacttgg ggtttttgac
     7141 atccgattga aggtactttt cgaataaagg aatggcctcc cgatattcat agcggtcgta
     7201 ataaatggtc cccaatgtaa aatatacccg ggcggaaggt ttaggattga ctgttaatat
     7261 ctgattgagg taaaatatgc tcgaatccat gtttttctgg gaaaaataaa tgcccgccaa
     7321 ttccatcatg gcatcctgaa aaccgggagt caactgcaag gcttccctat atttggcttt
     7381 tgctgttcca aaggactgtg ccatggctgc tttgcggcct tcatcgaaaa cctttttagt
     7441 tttatcactg gcgtctttga gagtaaccaa cggctgcgct attgcacacg actggatcaa
     7501 tccccctatc accaaaaata tcatcaatcg cttcacccta ttacaattgt atcaagatat
     7561 ttgtaaaaaa attccctcgt cctgtctatt cccaacatga aaagccattc aagtatggaa
     7621 gcctccggat tgatcccttc aacttccatc aataaaggcg actcaagaag attcggcact
     7681 tcaatgatct ttatgcctgc ttcaataaaa tcgttttccc gatggtattt attgccaccg
     7741 cgcccgctta aatacacatt tcctccaaaa tactgcatca gttcaacgat tcgcgccgaa
     7801 cccgatgatg aaacagggta gtcactgctg acattcaatt cttttctgat gtccatcaag
     7861 tccataatgt tgcgaatgat agctgtgttg taagttgcca gattggtagc cccgttattc
     7921 agccaagcct gcacacgagg aaacaattcc ttaaaacccg ctccggaccg atatctactc
     7981 tccagcctcc cggacagttc accttgccat tgtctccgat catccaacgg tatcttgttg
     8041 atacctgcat aggtatatcc ggatggaatt attaccggac accactccag caaaggatca
     8101 tttccgggtc ttctgatctt cgtccttcgg gtataccctc tccatggaat ctgcacatcg
     8161 tcaagcagga tgaaagtatc cgacaaagct atttttacaa aataaccaaa ccatggaaag
     8221 aaattaggct gatgaaccgc aacgatatgt gtatcctgtc ttttcaacat cttcttccgc
     8281 aaagttagtg caattgtacg ataattgaaa tccctgacat aaagcataat tacgggttaa
     8341 taaaaatgaa ataactattc acttcaaatc aaatatttag ttcaatca
//
LOCUS       DAOQQN010000104         8322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3342_length_8322_cov_5.201766, whole genome shotgun sequence.
ACCESSION   DAOQQN010000104 DAOQQN010000000
VERSION     DAOQQN010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3342_length_8322_cov_5.201766"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1552
                     /locus_tag="PLG24_05380"
     CDS             155..1552
                     /locus_tag="PLG24_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24355.1"
                     /translation="MDQNTDIKGSIEALKAIASEIQASDALATYQEEEDEDSYKELID
                     EFESRLMDIHVDVANHFPLQLEAFEREFIDPDLEGLLLPRLLGFSVLRGYHDKAYKYL
                     TPQDHFKNLLIAITGSPNFEFIKNRIGQTVQIGFALSSDIWITNLLEGIENKRVHQWL
                     LSQKLDKFYDPEVRKIAYVKYRNQFSSHNYFTAKIPTNMGELKLFYPSLKHFLFQRQR
                     LRLDNSNIKPAITTMLLNPIFSDTEEQHQILFILLNFFNLTGDDRESVKKLFNKLRKE
                     NKDSSDEYFDFLIEMYENRTILGPDSDKRVKDFLDTSIKDNILTYYELVDQLHSKGFM
                     HVDVIEAIKNYLNNYKGLSNEAEAIRQAVLRYFRQWIDNADVKDYPDYFELFKTFTIY
                     MDLFGNEHFNQQLEYSSLAYIRRLLKHYTDKRGKDYQDIKKFVQHTFTEVDFLKDKDA
                     VEMFKTKRKSKEIKK"
     gene            1876..3273
                     /gene="gndA"
                     /locus_tag="PLG24_05385"
     CDS             1876..3273
                     /gene="gndA"
                     /locus_tag="PLG24_05385"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HMZ24356.1"
                     /translation="MNTYSIGMIGLGTMGRNLLLNIADHGHTIIGYDKDATKADQVNA
                     IPGYDKISATSDITTFVSSLEKPARIILLVPAGPIVDYVINDLTPLLSEGDIIIDGGN
                     SYYKDTDRRYAALKSIGIHFMGMGVSGGEDGARFGPSIMPSGDKVAYDHVKQILDDVA
                     AKYKGMPCSKYMGNASAGHYVKMVHNGIEYGMMQLIAEAYDLMKNGLGYTNSRMADIF
                     EIWSRNKLEGFLVESAVKVLREKDTLTPMDLIDNIKDVARQKGTGKWTSQDALDLQVP
                     VPTIDLAVVARDLSGFEAERKAIHTLISGQKNKISGNITANELGKALRAAFLITYNQG
                     LHLIHAASTVYRYETSMVDVVNNWKAGCIIRSSLLTNLEKVYTNNPETALIISDPDIF
                     AIIRESQTALKKVAVFVIKNDFGASGFTSALTYLNMFSTERSPMNMVQGLRDLFGAHT
                     FERTDRPGSFHQQWK"
     gene            3302..4801
                     /gene="zwf"
                     /locus_tag="PLG24_05390"
     CDS             3302..4801
                     /gene="zwf"
                     /locus_tag="PLG24_05390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HMZ24357.1"
                     /translation="MANNKIPTTVFVIFGATGDLTYRKLMPALYNLELDNMLPENFSI
                     LGVGRSELTKDNLMEKLKEGTVKFSRRAVDDAVWNRLCSNFQYINGDFADQNLYDRVN
                     SAILDLKTAAQDKLNVIIYLAVSPTFFGPITKGLASFPFIKKRDQTRFVFEKPFGHDL
                     KSALDLNKEIMSVLRENQVYRIDHYLGKETVQNILAFRFANSFFEPLWNKNYIEWIQI
                     SSLEQVGLEGRGGYYDNSGALRDMIQNHMLQLMCMIAMEPPINFNADEIRNKKADVLH
                     AVRKYEGNEVFQNTVRGQYSAGKVSDELIPAYRDEANVRKDSNTETFAAALMYIDTWR
                     WEGVPFYIRTGKNLSQKATTIAVKFKPAPSYSFPREATGNWQSNLLLFNINPRMDIHL
                     KFQAKVPGQLMQLSQVNMEFDFDKELSANTPEAYEHLIYDVLEGDATLFMRSDQVEAA
                     WQIITPILDAWQNTKALEFPNYTPGSNGPVKADELLARYGHTWLNPGNI"
     gene            complement(5208..5837)
                     /locus_tag="PLG24_05395"
     CDS             complement(5208..5837)
                     /locus_tag="PLG24_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HMZ24358.1"
                     /translation="MDISTIFKNNEAWITNKLRNDPDYFGKLSSGQSPQLLYIGCSDS
                     RVSAEEIMGLGPGEVFVHRNIANLVNNIDLNTQSVIDYAVRALEIPHLVVCGHYKCGG
                     VEAALRPQNLGLLNPWLRNIRDVYRLHEKELNSIENEDQRYDRFVELNVIEQCINLIK
                     IASVQEAYNKGLVTIHGWVMDLKTGRIIDLGIDIEKEMTHIKEIYDLGT"
     gene            complement(5976..6398)
                     /locus_tag="PLG24_05400"
     CDS             complement(5976..6398)
                     /locus_tag="PLG24_05400"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMZ24359.1"
                     /translation="MNNNLKSNMTTSGLLITSMFIVLLTVTGLLIRPSEGVVNGKVVY
                     MDKTEVQDAVINVLAKNDLSLVSSQLTDDKGTFSIKGLAPGEYYISVQMEAQRQKVYG
                     PVFVAKGKQKVVIKPIIIPVQANASQAVVTASVITAAS"
     gene            complement(6996..>8322)
                     /locus_tag="PLG24_05405"
     CDS             complement(6996..>8322)
                     /locus_tag="PLG24_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HMZ24360.1"
                     /translation="YMIQPAGRPVSLSLSNGWSRFVNNEFAQGSGSNFIKAAKIGTPA
                     VVRITVRNKAIPIWDIGSLVSSGSGVVITADGYIATNHHVLAPGNLYEVTFNDGHKYK
                     ATLTGSDQSTDVAVLKIDRDDCPYLTMANSDSVQVGQWVIAIGNPYQLNSTVTSGIVS
                     AKGRTIDLLKGEYPLEYFIQTDAIFNEGNSGGALLNEEGELIGINTAIFTRSGKFEGY
                     SFAIPSNIVRKMVGDIISYGKVQRAVLGVGIQDITDRLARRIGEPIGSGVYINKVNPD
                     SPASEAGLRTGDIIISINGTNIRTYPALQEQVALYQPGDKVNIRYRRGGTIHIAEVEL
                     MPLKIESKKTVVRTDAPLKGIGLELRDDEGIPDPGIIVQDVIPGSSAARAGIKPGFKI
                     SEINGVVVTTIDQFINQLSKNQYRILLRGIEPESGLVTEYSIVKPVSGL"
BASE COUNT         2576 a         1780 c         1654 g         2312 t
ORIGIN      
        1 aaatgcgtga ttaatatttt tttaatcaat agagaaatat ttcacgaaaa tagtttaagt
       61 ttgtgcttga ctatttttct gtgttgaaat gaacctggta ggacctaaaa attctaccag
      121 gttcctattt tttaacccaa aattttaaat ataaatggat cagaatacag atatcaaagg
      181 cagtatagaa gcgctaaaag ccattgcttc cgagatccag gcatcggacg ctctcgcaac
      241 ataccaggaa gaagaagacg aagacagtta caaagagctg attgatgaat tcgaaagtag
      301 gctgatggat attcatgtcg atgtggccaa tcattttcct ttacaactcg aagccttcga
      361 aagggaattt atcgaccctg acctggaagg ccttctattg ccacgcttgc tgggcttcag
      421 tgtcctgaga ggatatcatg acaaggcata taaatatctg acacctcagg accactttaa
      481 gaaccttctg atagctatca ccggatcacc caactttgaa tttatcaaaa acagaatcgg
      541 ccagactgtc caaattggat ttgccctgag tagcgatatc tggattacga acctactgga
      601 aggcatcgaa aataaaagag tacaccaatg gttgctttcc cagaaactgg ataaatttta
      661 cgatccggaa gttagaaaga ttgcatatgt caagtatcgt aaccaattta gctcccacaa
      721 ctacttcacc gccaagatac ctaccaatat gggtgaactt aagttgttct acccatcatt
      781 gaagcatttc cttttccaac gccaacgact gagattggac aacagcaaca tcaagccggc
      841 tattacaacc atgttgctca accccatctt ctcagataca gaagaacaac accaaatact
      901 ctttatcctg cttaatttct tcaatctgac tggtgacgac agagaatctg tcaaaaaact
      961 attcaacaag cttaggaagg aaaataagga ttcctccgat gagtatttcg acttcctgat
     1021 tgagatgtac gaaaacagga ccatccttgg acctgatagt gataagcgcg taaaagattt
     1081 cctggatact tccatcaagg acaatatcct gacatattat gagctggtcg atcaacttca
     1141 cagcaagggc tttatgcatg tagatgtgat tgaggcaatc aagaattatc ttaacaatta
     1201 taaaggcctt tctaatgaag cagaagcaat acgacaagca gtattgaggt atttcaggca
     1261 atggatcgac aatgcagatg tcaaggacta tcctgattat ttcgaattat tcaagacatt
     1321 cactatatac atggatttgt ttggcaatga acatttcaac caacaactgg agtattccag
     1381 ccttgcctat atcagaagat tgcttaagca ttataccgac aagagaggta aggattatca
     1441 ggatatcaaa aaatttgtcc agcacacctt taccgaagtg gacttcttaa aagataaaga
     1501 tgcggtggaa atgtttaaga ccaaacggaa atcaaaagag atcaagaaat aacctgatcc
     1561 ctatataaaa ttaaaagctg ccattgtgac aatgacggct tttttaatgc cctcattcac
     1621 catcctttga tgaatggaag gaatatcatt atccgactaa taattggaaa atcacctgta
     1681 tcaagacgac catacctcca aacgctattt ttccatttaa cttcccatgc tatcttattt
     1741 tagaaaaatt tgaatctaca ggaaattttt ttgaaaaata tcaatacaga ataaaccctg
     1801 catagaatta attgtttcct tatcgaaaat tcaaattcat acttctgaat ttaacacatc
     1861 taactgacta gattcatgaa tacatattca attggcatga ttggcttggg gacaatgggg
     1921 cgcaatcttt tactcaatat cgctgatcat gggcacacca ttatcggcta cgataaggat
     1981 gctacaaagg cagaccaagt aaatgctata ccaggttatg acaagatttc tgccacttcg
     2041 gatatcacta cctttgtttc cagcctggaa aaacccgcca gaataatcct cctggtaccg
     2101 gcaggcccca ttgttgacta tgtaataaat gatcttaccc cattgctttc agaaggagac
     2161 attatcatcg atggtgggaa ttcttactat aaggacactg accggagata tgcagcattg
     2221 aaatcaatag gaatccattt catgggcatg ggagtatcag gcggtgaaga tggagcgcgt
     2281 ttcggaccaa gcattatgcc ttcaggcgac aaagtggctt atgatcacgt aaaacaaatc
     2341 ctcgatgatg tagctgcaaa atacaaagga atgccatgta gtaaatacat gggaaatgct
     2401 tctgcaggac attatgtcaa gatggtgcac aacggcattg agtatggcat gatgcaattg
     2461 atcgcagagg cttatgacct gatgaaaaat ggcctgggat acactaacag ccggatggcc
     2521 gatatctttg aaatatggtc acgaaataag cttgaagggt tcctggttga atctgccgta
     2581 aaagtactta gagaaaagga taccctcact ccaatggatt taattgataa tatcaaggat
     2641 gtagcaaggc aaaaaggtac aggcaaatgg accagccagg atgccctcga cctccaggtc
     2701 ccggttccta ccatcgatct ggctgttgtg gcacgagacc tgagtggatt tgaagcagaa
     2761 cgaaaggcaa tccatacctt gatatccggc cagaaaaata agatctcggg taacatcact
     2821 gcaaatgagt tgggtaaagc attgcgagct gccttcctca ttacctacaa ccaaggattg
     2881 catctgatac atgcagcttc aaccgtctac cggtacgaaa cttccatggt agacgttgtc
     2941 aacaactgga aggccggttg tattatcagg tcttccctgc ttaccaacct tgaaaaggta
     3001 tataccaata atcctgagac cgctcttatc atcagtgacc ctgatatatt tgccattatc
     3061 agggaaagcc aaacagcatt aaagaaagtt gctgtattcg tgattaaaaa tgatttcgga
     3121 gcatccggat ttacatccgc ccttacttat ttgaatatgt tttcaacaga aagaagtcca
     3181 atgaacatgg tacaagggct tagagatctt ttcggtgcac atacctttga aaggacagac
     3241 cggccgggat ccttccacca gcaatggaaa taatacttat caatattaaa taattgtctg
     3301 aatggccaac aataagatac ccacaaccgt ctttgtcatc tttggagcta ccggagacct
     3361 gacatatcgc aaactgatgc ctgcattata taatctggaa ctggacaata tgcttccgga
     3421 aaatttttcg atattaggtg ttggtaggtc cgaacttacc aaagacaatc tgatggaaaa
     3481 actgaaagaa ggtaccgtca aattctcaag acgtgccgtc gatgatgcag tctggaaccg
     3541 gctttgttcc aactttcaat atatcaatgg cgactttgcc gatcagaatc tttacgacag
     3601 ggtcaactca gccatccttg acctgaagac agcagctcag gacaagttaa atgtcatcat
     3661 atatcttgct gtctccccga cattctttgg tccgataact aaaggactgg catcatttcc
     3721 atttatcaag aaaagagatc agacacgatt tgtctttgag aaaccattcg gccatgacct
     3781 gaagagtgcc ctggatctca ataaggagat tatgtcagta cttcgggaga atcaggtcta
     3841 tcgcatcgac cattatctgg gcaaagaaac agttcagaat atcctggcat tccgttttgc
     3901 caattccttc ttcgagccgt tgtggaacaa gaactatatc gaatggattc aaatttcatc
     3961 cctggagcaa gtgggattgg aaggaagagg aggatattat gacaatagtg gtgccttgag
     4021 ggatatgata cagaatcaca tgctacagtt gatgtgtatg attgccatgg agccacccat
     4081 caacttcaat gcagatgaga ttagaaacaa aaaagccgat gtactgcacg ctgtacgtaa
     4141 gtacgaagga aatgaagtat ttcagaatac ggtccgcggt cagtactcag caggcaaggt
     4201 cagtgacgaa ctgattccgg catatcggga tgaagccaat gtcaggaagg attccaacac
     4261 ggagacattt gctgccgcct tgatgtacat cgatacatgg cgttgggagg gagtaccttt
     4321 ttatatcagg acaggtaaaa acctctcgca aaaggccact acaatagccg tcaaattcaa
     4381 accggcacct tcatacagct ttcccaggga agcaaccggc aactggcaat caaatctcct
     4441 gttattcaat atcaatcccc ggatggatat ccatcttaag ttccaggcca aggtgccggg
     4501 tcagttgatg cagcttagtc aggtcaatat ggaatttgat ttcgacaagg agctttcggc
     4561 caatactccg gaagcgtatg aacatttgat ttatgatgtg ttggaagggg atgccacttt
     4621 attcatgcgt tctgatcagg tggaagcagc ttggcagatc atcacaccaa tattggacgc
     4681 ctggcaaaac accaaggcgc ttgagtttcc gaattacaca cccggctcca atggaccggt
     4741 caaggcagac gagttactcg ccagatatgg tcatacatgg ctcaatcccg gcaatatata
     4801 accataaccc ctgagtaaaa agtcggtaag gcaataaaat aaaaacgttc ctgctataca
     4861 agaaaaaagg gaaacgatac ttttaccgct tcccttttag aaatttatta agcatcaccc
     4921 tttctgttgt tatatcctcc gcatttcaga ggattcaatc acatcgaatt aaattatcgt
     4981 gcttttgatg atgtaaagat gagacaataa atatttcctg ccttcagtgt atccggctaa
     5041 aataattttt catggtattt ctgtattttt ccaacaaacc tcgttaaggt aaatcaggtt
     5101 tctttacagg caggcatatt aatgaaatct accaaaaggg actatgaaag gatcctcagg
     5161 aagcttttac cggtgttttt cgaatgggta aaggtagtca ctccatctta ggtacccaga
     5221 tcgtaaatct ccttaatatg agtcatctct ttttcaatat caatcccaag atcgatgatg
     5281 cgaccggtct tgagatccat cacccagccg tggattgtga caagaccttt gttgtaagct
     5341 tcttgcacag aagcaatctt gataagatta atacactgtt caatgacatt aagctcaacg
     5401 aatcggtcat accgttggtc ttcattctcg atcgaattca gttctttttc atggagacga
     5461 tatacatctc gaatatttct gagccatgga ttcaatagac ccaggttttg cgggcgcaag
     5521 gcagcttcaa caccaccgca tttataatga ccgcaaacaa cgagatgagg tatttcaaga
     5581 gcacgtacag cataatcgat aactgactgg gtattcaggt cgatattgtt gaccagattg
     5641 gcaatgttac gatgtacaaa cacttcaccc ggaccaagtc ccataatttc ttcagcactg
     5701 acacgactat cagaacaacc tatatataat aactgcggag attgtccgga tgatagcttt
     5761 ccgaagtagt caggatcgtt ccgtaatttg tttgttatcc acgcttcatt atttttgaaa
     5821 attgtcgaaa tatccataaa ggagagatta ttcaacaaat ataacgaatc agacgtataa
     5881 ctctcagtcc aaattacctc taaaacagaa ataactacca gtgtcacatc aggcttgagg
     5941 gaacatggta gttataattt tttcaaatgc aactatcagg atgcggccgt aatcacagaa
     6001 gccgtaacaa cagcctggga tgcattggct tgtaccggaa tgataatcgg cttgatcacc
     6061 accttttgct taccttttgc aacaaataca ggtccatata ccttttgcct ctgagcttcc
     6121 atctgtactg aaatgtaata ttcacccggc gcaagacctt tgatggaaaa cgttcccttg
     6181 tcatccgtca attgtgacga aacaagtgaa aggtcattct tggccagaac attgataact
     6241 gcatcctgaa cctcggtctt gtccatatac accacctttc cgttgacaac cccttcggat
     6301 ggcctgatca atagaccagt tacagtcaat aggacgataa acatggaggt aatcaaaaga
     6361 ccggatgtgg tcatgttgga ttttaaattg ttattcatgg caaatataat tttgtaagtg
     6421 attaaatttt tgttccacaa agttaacgta ttttgacact aagtagttta tttattaata
     6481 tattttttta aaaaaatctt tatattactg ttattccgtg ttttacgtca ttaaaatatt
     6541 attttgaaat aaaaaattta aatataaaaa cacctgcaaa taagccatca ctcacacgtc
     6601 tgctgtctta atgcatcttg tttggaataa aaagcaaaat attcaaatat caagattcat
     6661 tttattgcca ttctgatatt tacatatata ttatatgtta tatttatatt aataacaata
     6721 tgataaattt ttggtaaatt tctatcggat tatagtttta tggcatataa caaaattata
     6781 ttttgactca aatttggatg aggaattttg ctctttcaaa ataaagaaag tacctattgg
     6841 tattcgtcaa cccaggtcat atgaaaactc cccgctgcac cctgaaaagg atggacacat
     6901 tagcaacaat aatctgaagg acctgataac tgatcattac ctacccaccg ggagtatgca
     6961 attcaattaa ttacaggaaa gaaaatgata atagtttata gccctgaaac cggcttgaca
     7021 atggaatact cagtgaccag accactctca ggctcaatac ctcgcagcaa gattctgtac
     7081 tggtttttcg atagctgatt gataaactga tcaatggtag taacaacgac gccattaatt
     7141 tcactgattt tgaagcccgg ttttattccg gcacgtgcgg cagaactacc ggggatgaca
     7201 tcctgcacaa taatgcccgg atccggaatc ccctcatcat cacgcagctc caatccaatt
     7261 cctttcagag gggcgtcagt cctaaccact gttttctttg attcaatctt aagtggcatc
     7321 aattcgactt cggcaatatg gattgtgcca ccccttctgt acctgatatt gactttatca
     7381 cccggttggt acagggcaac ttgctcctgg agggctggat aggttcttat attggtccca
     7441 ttgatagata tgatgatatc cccggttcga agtccggctt ctgaggcggg cgaatccgga
     7501 ttgactttgt tgatataaac tccagaacca attggctctc cgattcgccg cgcaagccta
     7561 tctgtaatat cctgaattcc gacaccgagt actgcacgct gcaccttgcc atagctgatg
     7621 atatccccaa ccatttttcg aacaatgttg ctgggaatgg cgaaggagta gccttcgaat
     7681 ttaccacttc gggtaaatat ggccgtgtta attccaatca actctccttc ctcattgagc
     7741 aatgccccac cgctatttcc ttcattgaag atggcgtctg tctgaataaa atattcaagc
     7801 gggtactccc ctttaagtaa gtcaatggta cgtcctttcg ctgaaacaat gccagaggtc
     7861 accgttgaat tgagctggta aggattacct atggcaatca cccactgacc cacctggaca
     7921 gagtcgctat tggccatggt cagatatgga cagtcgtccc tgtctatctt aaggacggcg
     7981 acgtcggtcg actgatcact gcctgtaagc gtagctttat atttatgacc atcattaaat
     8041 gtcacctcat aaagattacc gggtgcaagg acgtgatggt tggtcgcaat atagccgtca
     8101 gcagtaatca ccacccctga accggaggaa accagagaac cgatatccca gatcggtata
     8161 gccttattcc tgactgtgat tcttacgacg gcaggtgtgc cgattttggc agctttgata
     8221 aaattactac ctgatccctg tgcaaattca ttgttgacaa atcgactcca accattgctt
     8281 aaagaaagtg aaaccggccg ccctgcgggt tggatcatat ac
//
LOCUS       DAOQQN010000105         8303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3358_length_8303_cov_5.317168, whole genome shotgun sequence.
ACCESSION   DAOQQN010000105 DAOQQN010000000
VERSION     DAOQQN010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3358_length_8303_cov_5.317168"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="PLG24_05410"
     CDS             complement(<1..1222)
                     /locus_tag="PLG24_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ24361.1"
                     /translation="MKKTLLAFSLFIMVLISAYGQKRYLEPVFSGVSKNTVIYGHNFH
                     VLYVPVTGHTALQPLVADVYQPDGDTETARPLILYFHSGNFLPFPQNQSVSGTRSDST
                     TVEMSRRLAKMGYVVASCDYRLGWNPVAPEQADRVSTLINAAYRGVQDARTAVRYFKA
                     NAGTFGIDTSKIVLWGQGTGGYITLATSTLDSYTKVVTTTNPEGKFVAANGFPMVIEQ
                     INGNIWGTSFGIIPVGLGLPLEGDTLCIPNNVGPSSNFQLCVNMGGALGDISWLDANT
                     PPIISFQTTTDPFAPYHDDVLIVPGPNLPVVQVQGSYEVQKSAHNLGINKAFADKTFI
                     DPLSQYVSTINDGFEGLYPIFRANPYDSSPWDYWDRATNPNDASGILTNPDMSFAKAS
                     LFMDTIINYFAPRSC"
     gene            complement(1253..4081)
                     /locus_tag="PLG24_05415"
     CDS             complement(1253..4081)
                     /locus_tag="PLG24_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ24362.1"
                     /translation="MNKTLLLLWVKLIFVLIIVTVQTTSITAQNKFAARLMDKTTKQA
                     LIGATIIDQNNPSNNTISDENGIFYIERPVFPLSLKISYIGYSDMDFVLDKAPTSPVV
                     ITMEEEGQLLEGLVIKGQRVSDKQKADPRSVESMDIMAIKETPSISFYSGLGNLKGVD
                     LTTASLGFTIINTRGFNSTSPVRSLQLIDGVDNQAPGLNFSLGNFLGVSELDILKVDL
                     IAGASSTLYGPNAFNGVINMETKSPFYHKGLGASIKAGERNLLEAAVRWGDVIKNKKG
                     EDFFGYKLNFFHLRADDWVADNYNAVDGSRDTLGNPGGYDAVNVYGDEIDNRFKNTNL
                     IGYRGLTTWSRKGYKEVDLVDYDTRNTKANVGLYFRTKPSQGILSPEFIVSSSFGAGT
                     TVYQGDNRFSLKDILFFQNKIEFSKKNQYFIRAYATHEDAGNSYDPYFTALALQKSNK
                     NSEKFYSEFIRYWQTRINARAKANGYPIAEYDPMSGTFKFPPGSLEAWFPTHIDSLTA
                     WYNEAKNYADGLVVLPDQQGFYEVGTERFNQKFDEIVSSTSNDKGQKLGSRFYDKSAL
                     YHLQGEYKFTTEFGNITVGGSGRYYAPNSNGTIFYDTIGIKLSTYEYGIYAGYEKKLF
                     RDKLTANVAARVDKNKNFNFLVSPAASLVWNPSPNNYFRLSFSSAVRNPTLTDQYLYL
                     NVGPAILVGNLNGVDSVINVESFIDHLRDLSKPVQYFNIKPIKPEKVKSIELGARTTL
                     FDKIYVDAGYYFSIYDDFIGYNIGIKSDFENVTNLPKNTQVYRYAANSTNTVTTQGFN
                     LGINYYFANYFMFGGNYSWNKLNKTFEDDPIIPAFNTPEHKYNLSVSGRDVEIKLGKR
                     KLKNIGFNVTYKWLQGFIFEGSPQFTGFIESYDLLDAQLSLGINKLNSTLKIGASNVL
                     NNKVYQTYGGPKVGRMAYITILYNFEKK"
     gene            complement(4626..6023)
                     /locus_tag="PLG24_05420"
     CDS             complement(4626..6023)
                     /locus_tag="PLG24_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ24363.1"
                     /translation="MRHLLTVVIFLVTCFSAVAQDGNNKNALTFRALLADYFNPNGGD
                     LSDYKPYRYGYGFSYNRNLTSFLDVSVPFRFLNGRLKDQSTNTYVFGGDIQAQAKYYK
                     PGNKVIPYILSGFGGDYIKDEGVNFQIPVGAGVDIRMFDRGYFNFNTEYRFSLKTNRN
                     NFIHGIGFKYLLGKKNKLADMDSDGVVDSLDLCPDIKGLAAFNGCPDSDNDGVPDHLD
                     ECPDKPGSAKSKGCPDKDFDGIPDHLDKCPDQAGPIENQGCPVVDKDGDGIPDTEDLC
                     PDVPGMKSAMGCPDMDEDGVPDDKDECPDMPGLKIFKGCPDTDGDGVEDRYDRCPDIP
                     GSKANKGCPEIKKEDRQKLEFAMQAVQFELGKTTLLTSSYPILNDIADIMKRYPDYFL
                     TISGHTDPSGKIETNRKLSTNRAKACYNYLVSKGISTGRMEYVGYGPDKPRFDNSTEE
                     GRIKNRRVEFNLDLK"
     gene            complement(6344..6628)
                     /locus_tag="PLG24_05425"
     CDS             complement(6344..6628)
                     /locus_tag="PLG24_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24364.1"
                     /translation="MTKLNELVREIKEDGPSDVNLSKAKAAILEKRKEMLKTNSYWNM
                     KLIGMIYWGNNVDRFLDLNNVINKITVKDIQETARKLFDGKNEFIGIMNP"
     gene            complement(6674..>8303)
                     /locus_tag="PLG24_05430"
     CDS             complement(6674..>8303)
                     /locus_tag="PLG24_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24365.1"
                     /translation="ILAFSGSRVKPEVTLNDYRTRIVRSLFTQMLNKRYSDLAHSANP
                     PYIFASSDVGGIFTERYEQLLLMAMPSSDIKGTIDASVAEFLRAQKYGFNASEFELAK
                     KSTMSSIENRYNERNTTESSVLIQNFISNYLNGGPLPGIENEYRLYKEFLPGITLEEV
                     KAEASKWLSPNVTQDYFALLMGPQVESGNVPDSRGLLEMVKSAFRQTVEDNIEKKASE
                     NLIENLPAPGKVLSQQKNDLLGIVTYTLSNGVKVTVKKTDFKSDEILLEGVRKGGASN
                     FGKEDKSNVQFMSDVMEAMGYGNFTPTELGDALTGKNITLTQSMDEYSDKVSGSCSVK
                     DFSAFLELVHLQMTSPRLDKDLYEGYLSKMKMQLMFLKANPQVSFIDTLFKVMYNNNP
                     LRPIAVPTLDDVEKIDPARIVELYKQEFGNAYSFHFFLTGNVDESNIVPLLEKFIACL
                     PSTPYTPSFKDNGVDIIAGTHTFEYKKGENQKSLIIDVTHGPIPYTEDLELQTGIVGD
                     ILTIRLLEEVREKMGAIYGGTYVANIDRFPVSRY"
BASE COUNT         2545 a         1771 c         1413 g         2574 t
ORIGIN      
        1 gacaagaacg gggtgcaaag tagttgatga tggtatccat aaacaaggaa gccttagcaa
       61 atgacatatc aggattagtc agaataccac ttgcatcatt ggggttggtg gctctatccc
      121 agtaatccca tggagaggaa tcgtatggat tagccctgaa tatagggtac aaaccttcaa
      181 atccatcatt gatggtagaa acatactgag ataatggatc aataaaggtc ttatcagcaa
      241 atgccttgtt gattcccaaa ttatgggcac tcttttggac ttcataagaa ccttgtacct
      301 gaactacggg aagattcggt cccggaacga taagtacatc atcatgatat ggagcaaaag
      361 gatctgttgt tgtctggaag gaaatgattg gtggtgtgtt agcgtctaac caagaaatat
      421 caccaagggc acctcccata tttacacaaa gctggaaatt agaagatggt ccaacattat
      481 taggaataca aagggtatct ccttccaatg gaagacccaa acctacggga ataattccaa
      541 aacttgttcc ccagatattt ccattgattt gctcgattac cattgggaat ccgttagcag
      601 caacaaactt accttcagga ttggtagtgg ttactacttt agtataagaa tcaagcgtag
      661 atgtagccaa tgtaatgtag cctcctgttc cctggcccca taaaacgatt ttgctggtat
      721 ctattccaaa agtaccggca ttggctttaa aataccttac agcagtcctt gcgtcctgaa
      781 caccacgata tgcagcatta atcaaggtgc taaccctgtc ggcttgttca ggagcaacag
      841 gattccaacc cagacgatag tcacaggaag ctacgacata acccattttg gcaagtcgac
      901 ggctcatttc aacagtagta gagtcacttc tagtaccgct tacactctga ttttgaggga
      961 atggtaaaaa attacctgag tgaaaataca aaatcaaagg acgagcagtt tcagtatcac
     1021 cgtcaggttg gtatacatca gcaactaaag gctgaagtgc cgtatgtccc gtaacgggaa
     1081 catataaaac atgaaaattg tgtccataaa tcaccgtatt cttggaaaca cctgaaaaca
     1141 caggctcaag atatctcttt tgtccatagg ccgatatcag caccatgata aacaaagaaa
     1201 atgcaagtaa agtctttttc atcataattc tcattttaaa tttaattttt atctattttt
     1261 tttcaaagtt atataaaatt gtgatatatg ccatccttcc aacttttggt cctccatacg
     1321 tctgatacac cttattgttt aaaacattag acgccccaat tttcaacgtt gaattaagct
     1381 tattaattcc caaagacaat tgcgcatcca acaaatcata gctttctatg aatccagtaa
     1441 attgaggtga accttcaaaa ataaaacctt gtaaccattt ataagtaaca ttgaagccaa
     1501 tatttttaag ctttcttttt ccaagcttga tttctacatc cctgcctgat acagaaagat
     1561 tatacttgtg ttccggggtg ttaaaagcgg gaatgatcgg gtcgtcctca aaggtcttgt
     1621 tcaatttatt ccatgagtaa tttccaccaa acatgaagta attggcaaaa taataattga
     1681 tacccaggtt aaatccttgg gtggtgactg tgtttgtgga atttgcggca taacggtaca
     1741 cctgagtgtt tttaggtagg ttggttacat tttcaaaatc tgatttgatg ccgatgttat
     1801 aacctataaa atcatcataa atactgaaat aatatccggc atccacatat atcttatcaa
     1861 agagggttgt ccgggcacca agttctatgg acttgacttt ctccggtttt ataggtttga
     1921 tgttgaaata ttgaacaggc tttgacaaat ctcggagatg atcgataaat gactccacat
     1981 taattacact atccacccca ttcaggttac ctactaaaat agcgggtcct acattgaggt
     2041 aaagatactg gtcggtaagt gtcggatttc tgacagcgga tgaaaatgaa agtctgaaat
     2101 aattattcgg agaaggattc catactaagc ttgctgccgg tgataccagg aagttgaaat
     2161 ttttattctt atctaccctt gctgcaacat ttgcagttag cttatcccga aacagttttt
     2221 tttcataccc ggcataaata ccatattcat aggttgacaa ttttataccg attgtgtcat
     2281 agaatatcgt cccattgcta tttggagcat aatatcgtcc acttccacca acggtgatat
     2341 ttccaaactc tgtagtaaac ttgtattctc cctgcaggtg atataaggca gatttgtcat
     2401 agaacctact acccaatttc tggcctttat cattggaagt tgatgaaacg atttcatcaa
     2461 atttttgatt gaaacgctct gtaccgacct cgtaaaaacc ttgctgatca ggcaaaacca
     2521 ccagaccatc ggcataattc ttagcttcat tataccatgc tgtcaaagaa tcaatatgtg
     2581 ttgggaacca ggcttcgagc gatcccgggg ggaatttaaa ggtcccgctc atcggatcat
     2641 attcagcaat tggatatcca ttggctttgg ctctggcatt aatccttgtt tgccaatatc
     2701 tgataaattc actatagaat ttctcgctgt tcttgttgct cttttgaagg gcaagtgctg
     2761 taaaataggg atcatatgaa ttaccggcat cttcatgggt agcatatgcc cgaataaaat
     2821 attgattttt cttactgaat tcaatcttat tctgaaaaaa cagaatgtct ttcaaactaa
     2881 atctgttatc accctgatat acggtggttc cggctccaaa acttgatgat acaataaatt
     2941 cgggcgataa aatcccctga gatggtttgg tcctgaaata caaacctaca tttgccttcg
     3001 tgtttctggt atcgtaatcc accaagtcaa cctctttata cccttttctt gaccatgttg
     3061 tcaaacctct atatccaata agattagtat tcttaaatct attgtctatt tcatccccgt
     3121 aaacattaac ggcgtcgtat ccgcccggat tgcccaatgt atccctggac ccatcgacag
     3181 cattgtaatt atcagcaacc caatcatcag ctctaagatg gaagaaattc agtttatacc
     3241 cgaaaaagtc ctctcctttc ttatttttta tcacatcacc ccatcttacg gcggcctcca
     3301 gtaagttccg ttctcctgcc tttattgaag ctcctaatcc tttatgataa aatgggctct
     3361 tggtctccat attgattact ccattgaatg cattaggtcc gtataatgta ctacttgcac
     3421 ctgcaatcaa atcaactttc aaaatatcca attcagaaac accaagaaaa ttacccaatg
     3481 aaaaattcaa tccgggagct tgattatcaa ccccatcaat caactggagc gatctcacag
     3541 gactggtact gttgaagcct ctggtgttga taatggtaaa tcccagactg gctgtagtca
     3601 aatcaactcc tttcaagttc cccagcccgg agtaaaaact gattgaaggc gtctccttta
     3661 tcgccatgat atccatggat tctactgatc gcggatctgc tttttgcttg tccgataccc
     3721 gttgcccttt aatgaccagc ccctccagta attgtccttc ctcctccatg gtaattacta
     3781 ccggagaagt tggagcttta tccaatacaa aatccatgtc ggaataacct atataagaaa
     3841 tctttaagga taatgggaag acgggtcttt cgatataaaa gatcccattt tcgtcgctta
     3901 ttgtattgtt ggatggattg ttctgatcaa tgattgtcgc acctatcagt gcctgtttgg
     3961 tagtcttgtc catcaaacgt gcagcaaatt tattttgtgc agtaattgaa gtggtctgta
     4021 cagtaactat tattaaaaca aatatcaact taacccacag taacagtaaa gttttattca
     4081 taaaacggct taaaaggtta aaatgcttta aattataatt caatttataa aaatcagatc
     4141 caagataaga agtaaatatt attttcataa ttttattttt cattaaatat tataatatcc
     4201 tgatttagtg atataattca tttaaaacaa ttgaatttgg atgtattttt taccaacaaa
     4261 agtccatact gcaaaattat tcttcactat gttaaatgaa atacgtgcga tgatgaaaag
     4321 tagaatatca atttggacaa agtttgatta aatgtatttg cctgtattag tactattaat
     4381 caatcaaata cacataatca ataagtttaa taatttgata ttaaacaaaa ttatttaatc
     4441 tacatcttag tggattcttc aggttaaatc cctttgtaca accgctccct aaaatgcaat
     4501 gtatcaattt gccatctatt gaaacaagta taaaaaaaga aatgcacctc aaaatgaagt
     4561 gcattccagc aagtatttat cagcaagaat aaactcaaaa aactaataga gcttcttctg
     4621 cagcattatt ttaagtcaag gttaaattcc accctgcgat tcttgattcg tccttcttca
     4681 gtagagttat caaatctagg tttgtcagga ccataaccaa catattccat ccggcctgtg
     4741 cttatccctt tggataccaa ataattgtaa caggctttag ccctattggt ggataacttt
     4801 ctattcgtct caatttttcc agaaggatct gtatggccac tgattgtcag aaaataatca
     4861 ggataccgtt tcatgatatc cgcaatatcg ttcagtatgg ggtaacttga agtcaataga
     4921 gttgtcttgc caagttcaaa ctggacggcc tgcattgcaa actcgagctt ttgcctatcc
     4981 tctttcttaa tctccgggca tcctttattg gcctttgacc cggggatatc cggacacctg
     5041 tcatatctat cctctacccc atccccgtca gtatccggac agcctttgaa aatcttaagg
     5101 ccaggcatgt caggacattc atctttatca tccgggactc cgtcttcatc catatcgggg
     5161 catcccattg cagacttcat tccgggcaca tcaggacaca aatcttcagt atcgggtata
     5221 ccatctccat ccttgtccac aaccggacag ccctggttct caatcggtcc tgcctgatca
     5281 ggacatttat ccagatgatc cggaatacca tcaaaatcct tatccggaca accctttgat
     5341 ttggcagatc ccggcttatc agggcactca tcaagatgat cagggacacc atcattatca
     5401 gaatccggac aaccattgaa cgctgcaagc cccttgatat ccggacaaag atccaaagaa
     5461 tccacaaccc catcactatc catatcggcc aatttatttt tctttcccaa caagtattta
     5521 aaacctatac catggataaa attattccga ttagtcttaa gagaaaatct atactctgta
     5581 ttaaaattaa aatatccgcg atcaaacatt cttatatcga caccggcacc gacaggaatc
     5641 tgaaaattca ctccttcatc tttaatataa tcacctccaa atcccgatag aatataagga
     5701 atgaccttat tgcccggttt ataatattta gcttgagcct gaatgtcacc accaaaaaca
     5761 taggtattgg ttgattgatc cttcaaacgg ccattgagga agcgaaatgg aacagatacg
     5821 tcaaggaagg aagttaaatt cctgttgtaa gaaaatccat aaccgtatcg ataaggctta
     5881 taatccgata aatcccctcc gttgggattg aaataatcag ccagaagtgc tctgaaggtc
     5941 aaagcatttt tattattacc atcttgggca actgcactaa aacaagtcac caaaaaaata
     6001 actacggtca aaagatgcct catctttaat agtctcattt tgttttttgc gaaataacca
     6061 ttctgacaaa aatcagtatt ggtgatactc tttgttccat ccaataatta acccgtctgt
     6121 cgaattagca ttcagatgaa tacgccaatg cattgcaaaa atataaatat attttattat
     6181 atgtaaataa atattcaata atttttaatt ctaaaattac attacctatt caggtacaca
     6241 gtattttatc tgctaaggaa aagaaccacc tcagaaaaaa gagagtgaat tagttccact
     6301 ctcaattttt ctcagattat tccttctttt ccatttctat tttttacgga ttcattatgc
     6361 caatgaactc attcttccca tcaaacaatt ttctggcagt ctcttgtatg tcttttacgg
     6421 taattttgtt gatgacgtta ttcagatcaa ggaatcgatc cacattattg ccccaatata
     6481 tcattccaat caatttcata ttccaatagc tgttcgtttt taacatttcc ttgcgctttt
     6541 ccaatatggc agccttagct tttgaaagat tcacatcaga tggaccgtct tccttaattt
     6601 cacgtaccaa ttcattcaat ttagtcaaaa taggtgttac attttccggc ccgcattgca
     6661 actgagtagc gacctaataa cgagaaaccg gaaagcgatc gatattggca acataggttc
     6721 caccatagat ggcacccatc ttttctcgaa cttcctccaa tagcctaata gtcaatatat
     6781 caccaacaat tcctgtttgc agttcaaggt cttccgtgta aggaatcggc ccatgagtca
     6841 cgtcaatgat cagacttttt tggttttctc cttttttata ttcaaaagta tgggttccgg
     6901 caataatatc cactccatta tccttgaatg aaggcgtata aggtgttgat ggcagacatg
     6961 caataaattt ctccaaaagt ggaacaatat tggattcgtc gacattcccc gtgagaaaga
     7021 aatggaaact atatgcattg ccaaactctt gtttatacag ttcaacaatt cgtgccggat
     7081 caatcttttc cacatcatcc aaagttggca ctgctatagg tcgcaatgga ttattattgt
     7141 acataacctt aaacagggta tcaataaagg aaacttgcgg attagcttta agaaacatga
     7201 gctgcatctt cattttactc aagtacccct catacaaatc cttgtccaaa cgaggtgagg
     7261 tcatttgtaa atggacaagt tcaagaaaag cagaaaaatc tttgacagaa caactgcctg
     7321 aaaccttgtc ggagtattca tccatacttt gagttaatgt gatattcttc cctgttagag
     7381 catctcctag ttcagtgggc gtaaaatttc catatcccat cgcctccatc acatcactca
     7441 taaattgtac attgctttta tcctcttttc caaagttgga tgctcctcct tttcgaactc
     7501 cttcaagcaa gatttcatca gatttgaaat cagttttctt aacagttacc ttaacaccat
     7561 tactcaacgt ataagtaaca atcccaagta aatcattctt ttgctgactt aaaaccttac
     7621 ccggagcggg cagattttca ataagatttt ctgaggcctt tttttcaata ttgtcttcga
     7681 cggtttgtct aaatgcagac tttaccattt ccagcagtcc ccgggagtcc gggacatttc
     7741 cgctttcgac ttgaggaccc atcaacaggg cgaaataatc ctgagtcaca ttgggtgaaa
     7801 gccacttcga agcttcagcc tttacctcct ccagtgtaat tccgggcaaa aattccttat
     7861 ataatctata ttcattttca ataccaggca gtggcccacc attgagataa tttgagatga
     7921 aattttgaat caatacggat gattcagtag tatttcgttc attataacga ttttctatac
     7981 tgctcatcgt gctcttcttt gccaattcaa attctgatgc attgaagcca tatttctgag
     8041 cccgcaggaa ttctgcaaca gaagcatcaa tcgttccctt aatatcagag gagggcatgg
     8101 ccatcaataa aagctgttcg tacctctctg taaaaatgcc tcctacatca gaactggcaa
     8161 aaatgtaagg gggattagcg ctatgtgcaa gatcagaata tcgtttattc agcatttgag
     8221 taaaaaggga acgcacaatt cgagttctgt agtcatttag tgtcacttca ggtttgacac
     8281 gacttcctga aaaagctaat ata
//
LOCUS       DAOQQN010000106         8251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3387_length_8251_cov_5.156296, whole genome shotgun sequence.
ACCESSION   DAOQQN010000106 DAOQQN010000000
VERSION     DAOQQN010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3387_length_8251_cov_5.156296"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PLG24_05435"
     CDS             complement(<1..667)
                     /locus_tag="PLG24_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24366.1"
                     /translation="MTLPGLVMSQSRKKRNKTPQPVALTAPGGYQYDIFGSGSQLKVE
                     EGDLIYFNLQICHRDSVIEDSKKSIQVPEYVMPGKDLVVNDGPVASGLSVMALGDSLI
                     IYERIDTIAGLPETMKDWKEIIYKIKVIGVTKKSELEEVKLRIPAIQAIIEKDIDDYY
                     NQRFQKTLHTQSGVTVLVHEDGNGERFKPGEVTFVSFYAVSTKDRKYYGSTFESGRPF
                     QVRL"
     gene            complement(770..1609)
                     /locus_tag="PLG24_05440"
     CDS             complement(770..1609)
                     /locus_tag="PLG24_05440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HMZ24367.1"
                     /translation="MKKFSVLILFAMVMGMISCKNNAKDSVTKHGYPYTLFPTGSGDL
                     IQDDDVVFFDMQILHKDSILQDSRNNPIKPEFAIPADSLVTTPNPVIDALRLMRKGDS
                     VVVREKIDTVQGLPENMKDWKEITYRIKVTDVLNKKEKQKIKDMEPGVEKEVQKLITD
                     YNSGAIKDLKTTPTGLKYVILQDGNGPQIQKGEVAKVHYFGATVSNGKKFDSSFSRGS
                     TFTVPVGGGQVIPGWEEALLLMKKGTKAMVFIPGNLAYGANGIPGMIEPNSELAFYME
                     ILN"
     gene            complement(1630..2640)
                     /locus_tag="PLG24_05445"
     CDS             complement(1630..2640)
                     /locus_tag="PLG24_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HMZ24368.1"
                     /translation="MQEAKDLLQLLRTPRNIVVTSHRNPDGDAIGSSLALKSVLEKKF
                     HNVKVVLPSEYPFDVAWLEGIDDIIIYDRDQGRATDAIAAADVVFCLDYNSLSRVDKV
                     GEAIAASGARIVLIDHHLYPENFADISISDYEVSSTCELVYEVCKTLGWVNDLTVQAL
                     EALLVGIITDTGSFKYSFSPKTLVNTSEIIRLGGDMVYVQDKLFNHLPEKSLRLLGHC
                     LSKRMVIYPDKKAGLIFLSKKDYIDFNIQRGDTEGIVNHLLALDDVNVAAFITEQPTI
                     IKISLRSKGAFSVQQMASEYFKGGGHKNASGGSYYGSLKSAISRFEEAVDKYKEEIIH
                     SY"
     gene            3089..6025
                     /locus_tag="PLG24_05450"
     CDS             3089..6025
                     /locus_tag="PLG24_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HMZ24369.1"
                     /translation="MEWNSAEIEEKWKKIWIDSELYKIHTDPDKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIFSRYKRLNGFNVLHPMGYDAFGLPAEQFAVQTGVHPAISTQA
                     NINSFRKQLDNLGFSYDWSREVRTCDPSYYKWTQWIFLKMYNAWYDKSEEKSKDISIL
                     IDHFENHGTEGINSSCNSPLQFSASDWKNYSAKEQYDVLMNYRLMYRKISTVNWCEAL
                     GTVLANDEVKDGVSERGGFPVIKKPMMQWSMRTTAFAQRLLDGLDHVDWSDSLKIMQR
                     NWIGRSEGARVFFRLKGRVDNLEIFTTRPDTIFGATFMVLAPEHPLVNELTIQAQQKP
                     MFDYACETAKASEQERLNAQKQMTGVFTGSYAIHPFTGKDIPIWVSNYVLIEYGTGAI
                     MAVPAGDERDRRFAETFNLEIREIIQRNEDDDDIESKNGILINSDFLNGLPVNDAIIK
                     AIETIEKQGFGKRLINYRLRDANYSRQRYWGEPFPIAYDEDGVGHEISPLPLELPDIK
                     DFKPTGDGSSPIARATEWVNATPGLKRETDTMPGFAGSSWYFLRYMDPNNDTLPFSKN
                     AIDYWMDVDLYIGGAEHAVGHLLYSRTWHKFLYDFGLVPTEEPFKRLINQGMIQGVSE
                     KIYLLKESTDKLYFKNNSGQYEVKLLPQSVQVFVSHELIQDYPAKSTSEDKEMEGFSE
                     MSIHIDIVKDYGASTGGYLDKVAMEEFAVWRPEFASAVFVTYGGYYQNGTFHPVTDAD
                     AIRFNTKSEVEKMSKSKYNVINPDTVISEHGTDVFRMYEMFLGPLEQAKPWDTKGIEG
                     VNKFLRRFWNLFFDDNGNFALSEDTAPTESLKVLHKTIKKMDEDINKFSFNTCVSACM
                     IAVNEFKKQNVNHRVILEPMLVLLAPFAPFITEELWHLAGHKDSVHKAQWPAFNEKYT
                     VDDVVAYPICINGKKRDLCHFAIDADDDTIIKESLERPDVIKWMEGKQAKKVIVVKGK
                     MVNIVV"
     gene            complement(6069..7298)
                     /locus_tag="PLG24_05455"
     CDS             complement(6069..7298)
                     /locus_tag="PLG24_05455"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HMZ24370.1"
                     /translation="MTSRLNILFFIACLFVSFSAQSQQYVVAELQKGEGGYALLRRYG
                     LDISPCNLAEFYRINNLPYGSALSLGKKYNLPVRIYQYNKSSIRSTVKNFDLVKALEV
                     ERYNKWLKSQQIKSVYFLQDLKLWVPFYIYNCNYDNLAGASQPDNTPDKNLNTTQAEA
                     INKTEIKKNTLDIALFGNKHSQVEVLGNHLRGQVFYIKGGHGGPDPGAMAKIDDKIYC
                     EDEYANDIALRLGRKLIQYGAIVHFIVYDPDDGIRDDEYLPCDKDELHAGKRDIQVNQ
                     IQRLRTRVQIINDYYESYRKKGVKNQKFISIHIDSRNESLELDTHFYYQEGSKVGQEM
                     AVNTQAVFEKKFGELGSRKYTGTVKARDLYVVKYSRPPCLFVEVGNIQNVNDQKRILD
                     PDNRQTLADWLYEGITK"
     gene            7421..8071
                     /locus_tag="PLG24_05460"
     CDS             7421..8071
                     /locus_tag="PLG24_05460"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HMZ24371.1"
                     /translation="MKKVFLLVKKDLYSEMRNAHSISSIILYVFATVYIIYSAFINIQ
                     PQNWNVLFWIVFLFVSVNALLRSFDQENSNKALYFYQLAGPLQIFAAKFIFNTILLVL
                     LGLLTLALFSFVAGSPVRDYPLFLLTILLAGIGFASIFSFISAIGAKTGNSHMLTAIL
                     GFPCVVPVLLELLKLSANSLGLLNDTDFTTDILILGSIDIILLSLAFILFPYVWRD"
BASE COUNT         2517 a         1708 c         1537 g         2489 t
ORIGIN      
        1 caagtctgac ctggaaaggg cgcccggatt caaaagtact cccatagtat ttccgatctt
       61 tagttgatac ggcataaaag cttacaaaag taacttcgcc cggtttaaaa cgctctccat
      121 ttccgtcttc atgtaccaat acagttactc cactttgtgt atgaagagtc ttttgaaacc
      181 tttgattgta ataatcatca atatcctttt cgataatagc ctgaatagca ggtatccgca
      241 atttgacctc ttccagttct gactttttag ttaccccgat taccttaatc ttataaataa
      301 tttctttcca atccttcata gtctcaggca aaccggcaat agtgtctatc ctctcgtata
      361 tgatgaggct gtcgccaaga gccattacac ttagccctga agcaacagga ccatcattta
      421 ccaccaaatc ctttcccggc attacatact cgggcacctg aatagatttt tttgaatcct
      481 cgatcactga atcacgatgg catatctgca aattaaaata tatgagatct ccttcctcaa
      541 ctttcaattg gctcccgcta ccgaaaatat catattgata tccaccggga gcggtcaatg
      601 caactggttg gggagtctta tttcgtttct tcctgctttg tgacattact aatcctggta
      661 atgtcaaact gataagaatt aaaaataata ccctcatttg atttcgttaa attgcatcag
      721 cctctaccct gacagataga ggctgatgct acatcgaaaa attctgtcat caattcagta
      781 tctccatata gaatgcaagc tcagaatttg gctctatcat tcccggaata ccattggcgc
      841 cgtatgcaag attgccagga atgaacacca ttgccttagt gcccttcttc atcaacaaca
      901 atgcttcttc ccaacccgga atcacctgac ctccaccaac aggtacagtg aaggttgaac
      961 cccggctgaa agaactgtca aatttcttcc catttgatac tgttgctccg aaataatgta
     1021 ccttagctac ttcacctttc tgtatctgag gaccatttcc atcttgaaga atcacatatt
     1081 taaggccggt cggagttgtc ttaagatctt tgattgcccc tgaattgtaa tcagttatca
     1141 atttctgaac ttccttctcg actcctggtt ccatgtcctt gatcttctgc ttttcttttt
     1201 tgtttaatac gtcggtaacc tttatgcgat acgtgatttc cttccagtct ttcatgttct
     1261 caggtaaccc ttgaacagta tcaattttct ctcgtacaac aacactgtct ccttttctca
     1321 tcagacgcaa tgcatcaatt acaggatttg gagtagtaac caacgagtca gccggaatcg
     1381 caaattcagg tttgatcggg ttatttctgg aatcttgcaa aatggaatcc ttatgcagga
     1441 tctgcatgtc aaaaaacacc acatcatcat cctgaatgag atctccactt cctgtaggga
     1501 aaagtgtata agggtaacca tgctttgtca cactatcctt ggcattgttt ttacacgaaa
     1561 tcattcccat gaccatggca aataatatca atactgaaaa ttttttcata aaataaatta
     1621 cgattattgt taatatgaat gaattatttc ttccttatat ttatcaactg cttcttcaaa
     1681 tctggagatg gcgcttttaa ggcttccata gtaagaacca ccggatgcat tcttatgacc
     1741 accgcctttg aaatactctg atgccatctg ctgtactgaa aaagcccctt ttgacctaag
     1801 cgatatttta atgatcgtag gctgttccgt gataaatgca gctacattga catcatcaag
     1861 cgctagcaaa tgattgacta taccctctgt gtcgccccgt tgaatattga aatcgatata
     1921 atctttttta gacaaaaata ttagaccggc tttcttatcg gggtagatca ccatcctctt
     1981 ggaaagacaa tgtcccagca aacgcagaga tttctccggc aaatggttga acaacttgtc
     2041 ctgcacatat accatatctc cgcctagcct tatgatttct gaagtgttaa caagggtttt
     2101 cggcgaaaaa ctatatttga acgaacctgt atccgtgatg atacccacca acaatgcctc
     2161 taaagcttgt accgtaaggt cattgaccca tcccaaagtc ttacacactt cataaaccaa
     2221 ctcacaagta ctgctgactt catagtctga aatggaaatg tccgcaaaat tttcaggata
     2281 aagatggtgg tcgattaaaa caatcctggc acctgaagct gcaatcgcct cacctacctt
     2341 atccaccctt gaaagtgaat tataatcaag gcaaaagaca acgtcagccg cagctatcgc
     2401 atcagtagct cgtccttgat ctctatcata tatgatgata tcatctatac cttccagcca
     2461 ggctacatca aaaggatact ctgaaggcag aacaaccttg acattatgaa atttcttttc
     2521 aaggacggac ttcaatgcaa gagaagaacc aatagcatca ccatccgggt ttctgtgtga
     2581 tgtaaccacg atgttccggg gagtccggag caattgtaac aagtctttgg cttcttgcat
     2641 ataaaaacaa aactgttcct aacagcctga attacagata attaataata ttaatttttc
     2701 aaggttgaga aaacagccgc aaaaatcgta ttttttctat atataaaaaa atttaattta
     2761 gttgaatctc cattaaataa aggaatgttt tactttacaa tgaatatcct tcaaagattc
     2821 taatgaaaaa aagggaaaga aaagatctaa agacgtcaca ggagacacca ggccagggaa
     2881 taaaggctac cttgccagac aatatgagca atcataataa ttttaataaa aatagacact
     2941 caattaccct cccgatcaac ttcttaatta attaaaacgt catgtttctt caaaaacata
     3001 aaattttgcc tttgacttca ctctagttga gtattgataa atggtattct ttgtaccttt
     3061 gcagcttcaa aattgaaaat gcatagaaat ggagtggaat tcagcagaaa ttgaagaaaa
     3121 atggaaaaaa atctggatag attcggaact atataagatc catactgatc ctgacaagcc
     3181 gaaatattat gtgctagaca tgtttcctta cccttctggt gcagggcttc atgtcggaca
     3241 tccattaggt tacattgcat ccgatatctt cagcagatac aagcgcttga atgggttcaa
     3301 tgttctgcat cccatgggtt atgatgcatt cggccttccg gctgagcaat ttgctgtaca
     3361 aaccggtgta catccagcaa taagcacaca ggcaaatatc aatagcttcc gaaaacagct
     3421 ggacaacctt ggattttctt atgattggtc cagggaggtc aggacctgtg atccctccta
     3481 ttataaatgg acgcaatgga ttttcctcaa gatgtacaac gcctggtatg acaagagtga
     3541 agaaaagtca aaagatattt ctatccttat cgatcatttt gaaaatcatg gaactgaagg
     3601 tattaacagc tcttgcaaca gcccactcca attttcagca tcagattgga aaaattattc
     3661 cgccaaggag caatacgatg tgttgatgaa ttaccgactg atgtacagga aaataagtac
     3721 ggtcaactgg tgtgaagcac tcggtactgt tctggccaat gacgaggtta aggacggtgt
     3781 atctgaaaga ggaggcttcc cggtaatcaa aaaacctatg atgcaatgga gtatgcgaac
     3841 taccgcgttt gcccagaggc tgctggatgg gcttgaccat gttgattggt ccgattccct
     3901 taaaatcatg caacgcaatt ggatcggaag gtcagaaggc gcaagggttt tctttcggtt
     3961 gaaagggaga gttgacaatc ttgaaatttt cacaacccgt ccggatacaa tctttggcgc
     4021 tacattcatg gtacttgcgc cggaacatcc acttgtcaat gaactgacta ttcaggcaca
     4081 acaaaaaccg atgttcgatt atgcttgtga aacggcaaag gcttcagaac aagaaagatt
     4141 gaatgcccag aaacagatga caggtgtatt tacaggttca tatgccatac acccctttac
     4201 aggaaaggat attccaatat gggtctcaaa ctacgtcctg attgaatacg gcaccggtgc
     4261 catcatggca gtacctgcag gagatgaaag ggacaggagg tttgcagaaa cgttcaacct
     4321 tgaaatccgt gaaatcattc aaagaaatga agacgacgac gatatagaat caaagaacgg
     4381 tatcctaatc aactccgact tcctaaatgg gctacctgta aatgatgcta tcattaaagc
     4441 tattgaaacc atagaaaaac aaggttttgg gaagcgtttg atcaattata ggctcagaga
     4501 tgccaattat agccgccaac gatactgggg agagcccttc cctatcgcct atgacgaaga
     4561 tggagtcggg catgaaataa gcccattacc attggaattg cctgatatca aggattttaa
     4621 acccactggc gacggcagct cacccatcgc aagagcaaca gaatgggtca atgctacgcc
     4681 aggcttgaag cgtgaaacgg acactatgcc gggatttgca ggttcctcct ggtatttctt
     4741 gcgttatatg gacccaaaca atgacacgtt gccattttca aagaatgcca tagattactg
     4801 gatggacgtg gatctatata ttggcggagc agaacatgca gtcgggcatt tgttatactc
     4861 aagaacctgg cataagttct tgtatgattt cggccttgta ccgactgaag aaccattcaa
     4921 gaggctaatc aaccaaggga tgattcaggg agtatctgaa aagatttact tacttaagga
     4981 atcaacagac aaactatatt tcaagaataa ttccggacaa tacgaggtaa aattattacc
     5041 tcagtcggta caggtctttg tcagccatga gctaatacag gattatccgg cgaaaagcac
     5101 ctcagaagac aaggagatgg aaggtttctc tgaaatgagt atccatattg atatagtaaa
     5161 ggattacggt gcctccacgg gtggttatct tgataaagtg gcaatggagg aatttgcggt
     5221 ttggaggccg gaatttgcaa gtgctgtctt tgtgacatat ggcggatatt atcaaaacgg
     5281 aacctttcac ccggtaactg acgctgacgc catccgcttc aataccaaat cagaggtgga
     5341 aaaaatgtca aaatccaagt acaatgtcat caatccggac actgtaattt cagaacatgg
     5401 cacagatgta ttcagaatgt acgaaatgtt tttaggaccg cttgagcaag ccaagccatg
     5461 ggacacgaaa ggaattgaag gggtcaacaa atttcttcga cgtttttgga acctgttttt
     5521 tgatgacaac gggaacttcg ccctcagtga ggatacggcc ccaacagagt cattaaaggt
     5581 attgcacaag acgattaaaa aaatggatga ggatatcaat aagttttcat tcaatacctg
     5641 tgtgtcagcc tgtatgattg ctgtgaacga attcaagaaa cagaatgtca atcatcgagt
     5701 aattcttgag ccaatgctcg ttttattggc accctttgca ccatttataa cagaagaact
     5761 ttggcatctg gcaggccata aagatagtgt gcacaaagca caatggccgg cttttaatga
     5821 aaaatataca gtagatgatg tggtggcata tccaatatgt atcaatggta aaaaacgtga
     5881 tctttgccat tttgctattg atgctgacga tgatacgatt ataaaggaat ctcttgaaag
     5941 accggatgta atcaaatgga tggaggggaa gcaagctaaa aaggtaatag tagtcaaagg
     6001 caaaatggtc aatatcgttg tgtgaatttc cgggatgtca ggcacccgaa taatctggag
     6061 gtatgcgact atttcgttat accttcatac aaccaatctg ccaatgtctg tcggttgtca
     6121 gggtccagga tacgtttttg gtcattgaca ttctgaatat ttccaacctc gacaaaaagg
     6181 catggcggcc ttgaatattt gaccacataa agatctcgag ctttaacggt accggtgtat
     6241 tttctgcttc ccaattcacc aaatttcttt tcaaagacag cctgggtatt gactgccatt
     6301 tcctgaccga cctttgaccc ttcctggtaa taaaaatgag tatccaattc aaggctttca
     6361 ttccgactat caatatgaat acttataaac ttctgattct taacaccttt ctttctataa
     6421 gattcataat aatcattgat tatctgaaca cgtgttctga gtctttggat ttggttcact
     6481 tgaatatcac gttttccggc atgtaattca tccttgtcac aaggtaaata ttcatcatct
     6541 cttatgccat catcaggatc atatacaatg aaatggacga tagccccgta ctggatcagt
     6601 tttcttccaa gccttaaagc aatatcattg gcatattcat cctcacaata tattttgtca
     6661 tcaattttcg ccatcgctcc cggatcggga cctccatgac cacccttaat ataaaagact
     6721 tgtcctcgta aatgattgcc caatacttca acctgactgt gtttatttcc aaacaaagct
     6781 atatccaaag tattcttctt aatttcagtc ttatttattg cttccgcttg cgttgtgttt
     6841 aaattcttat ctggtgtatt atcaggctgt gatgcaccag caaggttatc ataattgcaa
     6901 ttatatatgt aaaaaggcac ccaaagtttc aaatcttgca aaaaataaac agacttaatc
     6961 tgctgagatt tcaaccattt attataacgc tctacttcaa gtgctttgac caaatcaaaa
     7021 ttttttacag tagaccgaat tgagctttta ttgtactggt aaattctgac aggcagattg
     7081 tatttctttc ccaatgaaag agctgaaccg taaggtaggt tattgatcct ataaaactcc
     7141 gccaggttac atggagaaat atccaatcca taccttctga gtaaagcata tcctccctct
     7201 cctttctgta attcagcaac aacatattgt tgactctgtg cgctaaatga aacgaagagg
     7261 caagctataa aaaataagat atttagtctt gacgtcaaac agctaatata aaatcacaaa
     7321 tatattacat ttatttataa tgtaaatcca ttctgagcta tttttcattc ttcaacaaaa
     7381 atatattata gatttgcttc agtaaatatt ttagattctg atgaaaaagg tatttctttt
     7441 ggttaaaaaa gacctttact ctgaaatgag aaatgctcat tctatcagtt caataatttt
     7501 atatgtattt gctacagtat acattatata tagcgctttt ataaatatcc aacctcaaaa
     7561 ctggaatgta ttgttttgga ttgtctttct attcgtatcg gttaatgcgt tattaaggag
     7621 tttcgatcaa gagaacagca ataaggcact ttatttttat caactagccg gacctttgca
     7681 gatttttgct gccaagttta ttttcaatac aatattatta gtactgttag gtttgttgac
     7741 actggcttta ttcagttttg tggcaggaag tcctgttcgc gactatccat tatttctgtt
     7801 gacaatttta ttggcaggta ttggctttgc ctccatattc tcattcatat ctgccatagg
     7861 agccaagaca ggtaatagcc acatgttaac agcgattctg ggattccctt gtgttgttcc
     7921 agtgttatta gagcttttga aattatccgc caatagttta ggtttattga atgacactga
     7981 tttcactact gatatcctta ttttaggttc catagacatt attctattga gcctggcttt
     8041 tattttattt ccttatgttt ggcgtgatta atcaagagcc atttctgtat gtgaaatagt
     8101 caaaaaagtc aatattgaaa tggtcatctt gcaatcaaca tcttccatgt tctgggcttg
     8161 tgacctatca ttcttgaaag tatatcgtcc aaaatttctc ctttcatgcg atgcctgtta
     8221 tatcggtaac agtattcatt gagataatcc t
//
LOCUS       DAOQQN010000107         8184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3422_length_8184_cov_6.399434, whole genome shotgun sequence.
ACCESSION   DAOQQN010000107 DAOQQN010000000
VERSION     DAOQQN010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3422_length_8184_cov_6.399434"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="PLG24_05465"
     CDS             <1..995
                     /locus_tag="PLG24_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HMZ24372.1"
                     /translation="TVPRLLEKVYEKIMAKGHELTGIRKSLFFWSVRVGSRYKLGHPG
                     SLIYRLQLRIARKLVFSKWLEALGGRVEFIVTGAAAMQPRLITIFSAAGINVIEGYGL
                     TETSPVLTVNRISERERCLGTIGMPIPGVEIKLAEDGEILARGLNIMKGYFNRPDLTA
                     EVIDSNGWFHTGDIGEWKEHFGNNFLKITDRKKELFKTAGGKYIAPQAIENKMKESRF
                     IEQIMVVGGDDYKYIGALIVPSFVQLNAWAVDMGISTSSNQNLINSPEVLALMLKEVE
                     RLNVHFGKWEQIKKIRLLSEEWTIESGMLTPTLKLKRKFLYARFKEEIADILQG"
     gene            1652..2467
                     /locus_tag="PLG24_05470"
     CDS             1652..2467
                     /locus_tag="PLG24_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24373.1"
                     /translation="MRCKNCGANNSVHALKCIHCNVPLVGSMVVDMNISDPKISDGII
                     CNNCKSINPPNATNCRQCKAQLANPRFSGFEKSSDQLEIKSESAPPAPQQRKPRRNVC
                     PVCSYPNQPISNECVKCHTPLLSVNPTVPKDEPSKEINTQKQPAPNKIPERKEPDQQA
                     DSLNMTINPWAQQPVIPDEFCLIPLKSDYSASGDPISLVNAENNLNRDKLDPGNMTIT
                     SSSQAIVQQRGGKWLIRDTSSMQTTFVKVQGEMELRDGDIILMGNKIFKFSRK"
     gene            2467..3720
                     /locus_tag="PLG24_05475"
     CDS             2467..3720
                     /locus_tag="PLG24_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HMZ24374.1"
                     /translation="MISAANINEQSGRQILDSLDVIGDYIIEGTLGEGRFGIVYKAKH
                     RNTGHPVAVKLLKVWSVPQHAQMNLIRRFELEFETGQIKSDHLVHTIEMDQIEGIPFI
                     VMDYYPGGNLERKINAGITQDEAIVIAKGILRGLNDLHRNGKIHRDLKPENVLFDDQG
                     RPRLTDFGISGHINIQLTVVNQDDHPEQVFGSYAYMAPEQLSPIKRKNTLLPTIDIFS
                     YGVLCFEMFHNSLPFGPWKSHDDIDPYVRRAASGMTGPIKEDLSPIWKEILIKCLNPD
                     RERRFQNTDAIFALLRPGASDEKQPVKSSFTLQILDGEEYDRQYHIRLDGFARMGRAT
                     SFNDNEIEIMETISNYISRRHATFETIGSAVYLRDGQWIEDGGNWKFSKNGTYVNGLK
                     IDDEEGFQLYQNDIITIGNTSIKII"
     gene            3743..4726
                     /locus_tag="PLG24_05480"
     CDS             3743..4726
                     /locus_tag="PLG24_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24375.1"
                     /translation="MRRAKEFHLVNISFIDMLAGALGATIILFIIVPKMSFFDLKKLK
                     ALDSLDVKKAHMDSMVAKLESIVPEKEYRDLVASSVSLQSSIENLKSEIRTLQNTLNV
                     KTIQYSHVAQKYDRAMQTIRTIQQQLAGNPDDEELKRIQAELKAAQTALSPGPTDVSL
                     VANGKQSSPGITPPDASLSRGDAAMGKEPPLIILINWDDKDDKVHLYMRSAGTSNWVF
                     YQNNHRKAGFGTWDDNLANYEDLPYEAIVQNDNLVPGTYEIYAQTASAKTGSVEVSGF
                     ISMKINDGPVKRFNILSRKINVAKPPFEDNSGSTKLGTLTVSGDDIGWNPN"
     gene            4758..5495
                     /locus_tag="PLG24_05485"
     CDS             4758..5495
                     /locus_tag="PLG24_05485"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HMZ24376.1"
                     /translation="MNTRFTNFILTIILTTALGLILTIFYSFFPNRLLKLLGGEVPYG
                     LIQLATYFFFIFGMLEVLRFKKFVEFQKKGLSLNLLPEKEQFVISADEVNDIKLKMIE
                     LEKSNPLLLVDMIKKACTKFRSNKNVSESLSIVSVQTEINLRNTDSEQNLIKYFAWAC
                     QSIGLIGTVIGIGASLRAANQISSQEGIDYVTGLLSVSFDTTLIALVLSIILMYAYSL
                     ISERIDRLHADNQIYVIENLINRIYNK"
     gene            5516..6772
                     /locus_tag="PLG24_05490"
     CDS             5516..6772
                     /locus_tag="PLG24_05490"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HMZ24377.1"
                     /translation="MLFQFEAIVDIGLSRSVQQDNTLTMPQLILNNQCLTDEYFYDDI
                     NGALFAVADGMGGGPHGELASRLVVDGLRHFAVCNKHLLESNVITFTNNAIYEANQRL
                     LDHLNEHPEDNRMGSTVVLAIVRNGVLYLGWVGDSRCYLVNGNSIKVLSHDHSLVQSL
                     IDEGSITEEEAFYHPNRNIINQSMGMSNIIPSFEKYSLEDNQILLLCSDGLNGMLRDF
                     EILEICNRHSDLKVMVQSLVDAANKAGGHDNISCIGIRLKDYPARAKIFIKNEHPAGS
                     KKSENKIPLKLILLSIFLIIAFIWVFLVLKSGNDTSLQENDRIHSRQTMPVSPDTTAE
                     EDIGMPVESQGFYFIRLKGFNDSLKAQEYLEQLSNQYNNYVFMINYSSNALYEVTVNG
                     FKDEDEANKFIASGQFPSAIIKLQKE"
     gene            6775..>8184
                     /locus_tag="PLG24_05495"
     CDS             6775..>8184
                     /locus_tag="PLG24_05495"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HMZ24378.1"
                     /translation="MNISEVFHSRYELLQRVGRGGFSEVWKALDMRSGVEVAIKVFRQ
                     QDSEGIALCREEYLKTFDFQHPNILTPFHFDVSDDRPYLVMKFLNGGTLADHLGNMNA
                     AKVEDLIRQLGSALKYLHSRPVPVIHGDIKPDNILIDENGNYFLTDFGISTKLIQRFT
                     ETLRADSIVDSSKGVTPMAYRSPESFKYKNWEFNKTGPFSDIWSAGVTVYQAIYNSLP
                     FNGEGGLGQLILMKSGNHSLEEIIEFEDGDFTRFNAIILAALQLNPQQRATFLGEVVD
                     SPEPIITSESISDPPLLIDDTEGISIRKKKESRAFLYPLLAIFAIGVVLLVIALSKGG
                     KEKEGNNPPALSQEAIQTVDSIQDQTVDNSDHNVNDKLHATRDISTDEKIAANLRTEA
                     KTVPVNNSSPAIQTNTPEENHTTPIDQSENVSSAKENIQSEKAEPVSVETRIMNPSES
                     REKTKSRSVTIKPNIPIPLM"
BASE COUNT         2515 a         1583 c         1760 g         2326 t
ORIGIN      
        1 ctactgtgcc gcgtttactt gagaaagtct atgagaagat catggccaaa ggtcacgagc
       61 tgacaggcat caggaagagt ttgtttttct ggagcgttag ggttgggagc cggtataagc
      121 ttggtcatcc ggggagtttg atttatcggt tgcaattacg aattgcgcgg aaactggtgt
      181 tttcaaaatg gctcgaagcc ttgggcggac gtgtagaatt catagtgacc ggtgccgcag
      241 cgatgcaacc gcgactcatt acgatcttca gtgccgccgg gattaatgtg attgaaggct
      301 atggcttgac tgagacgtct cctgttctaa ctgtcaaccg catctccgaa cgtgagcggt
      361 gcctggggac catcggtatg ccgattcccg gtgtggaaat aaaattggca gaggatggtg
      421 agatcctggc cagaggcctc aacatcatga aaggatattt taatcgtccc gacctcacag
      481 ctgaagtgat cgatagcaat ggttggtttc atacaggcga tatcggtgag tggaaagagc
      541 attttggtaa taattttctc aagattacgg atagaaaaaa agaacttttc aagacggcag
      601 gtggtaagta tatcgcaccg caagcgattg agaataagat gaaggaaagt cgcttcatcg
      661 aacaaatcat ggttgtcggc ggtgacgatt acaaatatat cggtgcattg atcgtgcctt
      721 cctttgtaca actaaatgcc tgggcggttg atatgggtat cagcacatct tccaatcaga
      781 atttgataaa tagtcctgaa gtgttggcac tgatgttaaa ggaagtcgaa cggctcaatg
      841 tccacttcgg caagtgggag caaattaaaa aaatccgcct cctttctgaa gagtggacca
      901 tcgaatccgg tatgcttaca cctaccctta agctgaaacg caagtttctt tatgccaggt
      961 ttaaagagga aatagctgat atattgcaag gttgatgaga tgttgcttct gcaatggtat
     1021 caaatttcta cggtgattgt tttagaccgt ctgttaggac tacggaagca ggtaagtcat
     1081 tttgaatcaa taatttaact attgattcgc tgggaagaag ataatttaaa agatggcctt
     1141 accaaagagc aatttttttc acgtctttga aatgagctga ttcccgatgt tggtggatta
     1201 ttattctcct gcctaatatg tgggcaacta atgatcaata aatctgattg acaagatgtt
     1261 tttgatgttg aattggcaat tttacagtac actcccaatt tacctgtcag gaaactctga
     1321 cattttatca agcaggaagg aaagtacatc cggataacat tttaaattct gtaatgaaag
     1381 gtgagaaaat ttgattacat ttggcatgaa tacatggttt aaaggccact tttagtcaac
     1441 tatcagttat ttttacttgc agattttggc ttgattgtac ttgtaattag aggaaggcgg
     1501 gatatatatt gaaagcttga ttttatttgg cctgaatact tgattggtaa ggtcagtaca
     1561 attcagatta cttatagacg atttttgtaa atttcattta cctgactttt ttatttggga
     1621 gttgatattt tcagttttaa attatagtat catgagatgt aaaaactgcg gtgcaaataa
     1681 tagcgtccat gccttgaaat gtattcattg caatgtgcca cttgttggat caatggtggt
     1741 tgatatgaat atttccgacc ctaaaatctc cgatggtata atctgtaata attgcaaatc
     1801 aatcaatccc ccaaatgcga ccaactgccg gcaatgcaag gcacaattgg ccaatccccg
     1861 gttttccgga tttgaaaaat cttcggacca gttagaaatt aagtcagaaa gtgcacctcc
     1921 ggctcctcag caaagaaaac ccagacgaaa tgtatgtcct gtttgctcct atccgaatca
     1981 gcccatttct aatgagtgtg tcaaatgtca tacaccattg ttatccgtaa atccgacggt
     2041 gccaaaggat gagccatcca aagaaatcaa tacccaaaag caaccagctc caaataagat
     2101 cccggaaagg aaagaacctg atcaacaggc agattcttta aacatgacca tcaatccttg
     2161 ggcacaacaa cctgtcatcc cggatgaatt ttgtttgatt cctttgaaaa gtgattattc
     2221 cgcttcaggt gacccaatct cattggtaaa cgctgaaaat aaccttaatc gggacaaact
     2281 tgaccccggc aacatgacca tcacttcatc cagtcaggca attgtgcaac aacggggtgg
     2341 aaaatggctc attagggata ctagcagcat gcagactacc tttgtaaaag tccagggaga
     2401 gatggaatta agggacggag atatcatatt aatgggcaat aagattttta aattttcaag
     2461 gaaataatga tctccgcagc caatataaac gaacaatcag gccgccagat cctggattcc
     2521 cttgatgtta tcggagatta catcattgaa ggaacattgg gggaaggtag gttcggtatc
     2581 gtctataaag caaaacaccg gaatacggga catccggtcg ctgttaagtt gctgaaggtg
     2641 tggtcggttc ctcaacatgc ccaaatgaat ttgatcagaa gatttgaatt ggaatttgaa
     2701 acgggacaaa tcaaaagtga tcatctggtg cacactattg aaatggatca gattgaaggt
     2761 attcctttta ttgttatgga ttattatccc ggaggcaacc tcgaaagaaa gattaatgcc
     2821 gggattacac aggatgaagc tattgttata gccaaaggga ttctccgggg attaaacgac
     2881 cttcatcgta atggaaaaat tcatagagac ctaaaaccgg aaaatgttct ttttgatgat
     2941 caaggacgtc cacgactgac tgattttggc atatccgggc atatcaatat tcaattgact
     3001 gtggtcaatc aggatgatca ccccgaacag gtgttcggta gttatgccta tatggcacct
     3061 gagcaacttt caccaatcaa aagaaaaaac acgctgttgc ccacaatcga catcttttcc
     3121 tatggagtcc tgtgctttga aatgtttcac aattcattac ctttcggacc ctggaagtct
     3181 cacgacgata ttgatccata tgtcagacgc gcagcaagtg gaatgaccgg cccgataaaa
     3241 gaagatctat ctccgatatg gaaagaaatt ctcattaaat gccttaaccc ggacagggaa
     3301 cgtcgctttc aaaatacgga cgcaattttt gctcttctaa gaccaggtgc atcggatgaa
     3361 aagcaacctg tcaagtcctc atttaccttg cagatattgg atggtgaaga atatgacaga
     3421 caatatcata tccggctgga tggatttgca cgtatgggta gagcaacttc cttcaatgat
     3481 aatgaaattg agattatgga aaccatttca aactatatat cccggcgaca cgccaccttc
     3541 gaaaccattg gatccgcggt atatctaaga gatggacaat ggatagaaga tggaggtaac
     3601 tggaaatttt caaaaaatgg cacctatgtc aatggcttga agatagatga tgaagaagga
     3661 tttcaactat accaaaacga tattataact atagggaata catcgataaa aattatctga
     3721 gtattcattt taatagaata ttatgcgtag agcgaaagaa tttcatcttg tcaatatttc
     3781 ctttattgac atgttggctg gagccttggg cgcaacgatt attttgttta ttatagttcc
     3841 caaaatgtct ttttttgatt tgaaaaaatt aaaagcactg gactcactgg atgtcaaaaa
     3901 ggcacatatg gacagtatgg ttgctaagct tgagtccatt gtacctgaaa aggaatatcg
     3961 cgatctggtc gcaagttctg tgtcccttca atcttccata gaaaatttaa aatctgaaat
     4021 cagaacacta cagaataccc tgaatgttaa aacaattcaa tatagccatg tcgcacagaa
     4081 gtacgatcgt gctatgcaga caatcagaac catacagcaa cagcttgcgg gtaatccgga
     4141 tgacgaagaa ttaaaaagga ttcaggcaga gttaaaggct gctcaaactg ctctcagtcc
     4201 gggtcccact gatgtttctt tagtcgccaa cggcaaacag tcatccccgg ggataactcc
     4261 acccgacgct tcacttagca ggggtgatgc ggccatgggc aaagagcctc ctttgataat
     4321 tttgatcaat tgggatgata aggatgacaa ggtccacctt tatatgcggt cagcaggtac
     4381 aagcaattgg gttttttatc agaataacca tagaaaggcc ggtttcggaa cctgggatga
     4441 caacttggca aattacgaag accttcccta tgaagccatt gttcaaaatg ataacctcgt
     4501 accgggaact tatgaaatat atgctcaaac ggcctccgct aagacaggta gcgtggaggt
     4561 ttcaggtttt atttcgatga aaataaatga tggaccggtc aagagattca atatattaag
     4621 ccggaagata aatgttgcca aacctccttt tgaagacaac agtgggagca ccaaactagg
     4681 cacattaacg gtttccggtg atgatattgg ttggaatccc aattgattta cttgtttgaa
     4741 aatttgatat aattgaaatg aatacaagat ttaccaactt cattctgaca atcatcctta
     4801 ctacagcgct gggacttatc ctgactatat tctattcatt ttttcccaat cgtttgctga
     4861 aactcctggg cggggaagtg ccttatggat tgatccagct ggctacctat tttttcttta
     4921 tatttggtat gttagaagtt ttgcgtttca agaaatttgt tgaatttcag aagaaaggac
     4981 tttcactcaa tttgttgccg gaaaaggaac aattcgtcat atcagcagat gaagtcaatg
     5041 atatcaagtt aaaaatgatt gaattggaaa aatctaaccc tcttctcctt gtggatatga
     5101 tcaagaaggc ttgtaccaag tttcggtcca ataagaatgt cagcgaatca ctcagtatcg
     5161 tttcagtcca gactgaaatc aatctgagga ataccgattc agagcaaaat ctgattaaat
     5221 attttgcatg ggcatgccaa tctataggac tcatcggtac tgttataggt atcggtgctt
     5281 cactcagagc ggccaaccaa atcagcagcc aggaagggat tgactatgta accggtctgt
     5341 tgagtgtttc atttgacact acattgatag ctcttgtctt gagcattatt ctcatgtatg
     5401 cctacagcct gatatcagaa aggattgata gattacatgc tgacaaccag atctatgtta
     5461 tcgagaacct catcaatcga atctataaca aataactgcc ataaatgatt tagtcgtgct
     5521 atttcaattt gaagccatcg tcgatatcgg attaagccga tctgttcaac aggataacac
     5581 gttgacgatg cctcagttga ttctcaacaa ccaatgcctt acagatgaat atttctatga
     5641 tgatatcaat ggcgctctct ttgcagtcgc agatggcatg ggcggtggac ctcacggaga
     5701 attagcatcc agattggtcg ttgatggcct ccggcatttt gctgtttgta acaaacatct
     5761 cctggagagt aatgtgatta ccttcaccaa taatgccatc tatgaagcta accaaaggct
     5821 gctggatcac ctgaatgaac atccggagga taatagaatg gggtcaacgg tggtacttgc
     5881 tattgtcagg aatggtgtgc tgtatttagg ctgggtaggc gatagtcgtt gttatttagt
     5941 caatggaaac agcatcaaag tattgtcaca cgatcattcc ttggtgcaga gcctcataga
     6001 tgaaggctct attacagaag aggaggcttt ctaccatcca aatcgaaaca tcatcaatca
     6061 aagcatgggc atgtcaaata ttattccatc ctttgaaaaa tatagcctag aagataatca
     6121 aatcctttta ttgtgctcgg atggccttaa tggtatgcta agagattttg aaatccttga
     6181 aatatgcaac agacacagtg atctgaaagt tatggtccaa tctctggtgg atgctgccaa
     6241 caaagcggga ggacatgaca atattagttg tatcggcatt cggttaaagg attatcctgc
     6301 cagggctaag attttcatta agaatgaaca tcctgccgga agtaaaaaga gtgagaataa
     6361 aattcccctc aaattaatcc tcctcagtat ttttttaatc attgccttta tttgggtctt
     6421 tctagtacta aaatctggta acgatacttc tttacaggaa aatgacagaa ttcattcccg
     6481 gcaaacaatg cctgtctcac ctgatactac tgcagaggaa gacatcggta tgcctgttga
     6541 atctcaggga ttctatttta ttaggctgaa agggtttaat gatagcttga aagcacaaga
     6601 atatctcgaa caactatcaa atcaatataa caactatgtt tttatgatca actatagttc
     6661 aaacgcactc tacgaggtta ctgtcaatgg cttcaaggat gaagatgaag caaataaatt
     6721 cattgcatcc ggacaatttc ccagtgcaat aatcaaatta caaaaggaat gacaatgaat
     6781 atatcagagg tgtttcattc aaggtatgaa ttgctccaaa gggttggcag gggaggcttt
     6841 tctgaagtat ggaaggcgct cgatatgcgt tccggggtgg aggtcgccat caaggtgttc
     6901 aggcaacagg attcagaggg tattgccttg tgcagagaag aatacctcaa gacttttgat
     6961 tttcaacatc ctaatattct tacccctttc catttcgatg tctcagacga tcggccttat
     7021 cttgtcatga aatttctgaa cggaggcaca cttgccgatc acttgggaaa tatgaatgct
     7081 gcaaaggttg aggatcttat ccgtcaattg ggctcggcac tgaaatatct acacagtcgc
     7141 cctgttcctg tcattcacgg ggatattaaa cccgataata ttctcataga cgaaaatggt
     7201 aattactttc tcactgattt cggtatttct accaaattaa tacaaagatt taccgaaact
     7261 cttagagcag actctattgt agattcaagt aaaggagtta ctccgatggc ataccgcagt
     7321 ccggaatcat ttaagtataa aaactgggaa tttaacaaga ccgggccttt ttccgatatt
     7381 tggtcagcag gtgttacggt gtatcaggct atttataatt cattgccttt taatggtgaa
     7441 ggtggtctgg gacagctgat tctgatgaaa agtggtaatc attcgctgga ggaaatcatt
     7501 gagtttgaag atggcgattt tactcgcttc aacgcaatta ttctggctgc attacaattg
     7561 aatcctcaac aacgtgctac ttttttgggt gaagttgtgg atagcccgga acctatcatc
     7621 acatcagaat caatatcaga tccgcctcta ctcattgatg atacagaagg catttcaata
     7681 agaaaaaaga aagaatcccg tgcttttctt tatcccttgc ttgcaatatt cgccattggt
     7741 gtagttttat tggtaattgc cctttctaaa ggaggcaaag agaaagaggg aaacaatcca
     7801 ccggcgcttt cacaggaagc tattcagact gttgattcaa ttcaggatca gactgttgat
     7861 aatagtgatc ataatgttaa tgataaatta catgctaccc gggatatttc caccgacgag
     7921 aaaattgccg ccaatttgag gactgaagca aaaaccgttc ctgtaaataa ttcttccccg
     7981 gccatacaga ctaatacacc ggaagaaaat catacaactc cgattgatca gtctgaaaat
     8041 gtctcttccg ccaaggagaa cattcagtct gaaaaagccg agcctgtatc agtcgagaca
     8101 cgaataatga atccatcaga gagcagggag aaaactaaat ccaggtcagt cactataaag
     8161 cccaatatac ccataccatt gatg
//
LOCUS       DAOQQN010000108         8156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3439_length_8156_cov_6.412048, whole genome shotgun sequence.
ACCESSION   DAOQQN010000108 DAOQQN010000000
VERSION     DAOQQN010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3439_length_8156_cov_6.412048"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="PLG24_05500"
     CDS             <1..1201
                     /locus_tag="PLG24_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ24379.1"
                     /translation="VETGINAASGTHLGYIPGGGIFAAALADFIAAVTNPYAGVYYAS
                     PGAVTIENEVIHWLKSIFSFPADAVGNLTSGGSVSMLIAFNAARDKYGVKNEKVRQSV
                     VYLSQQVHHSAQKALRILGLEDVIIRYVPLDDRHRMNADILALLVAEDKSRGLHPFLV
                     VATAGTTDTGAVDPLDAIADITEMHNLWFHVDGAYGGFFILTSKKELFRGIERANSII
                     VDPHKSLFLPYGLGAVLIRDKDAVLHSNRYAANYMQDALGDELVKSPSNLSPELTKHF
                     RGLRMWLPLQLHGLAPFIACLEEKLLLTAYFRAKMVAVGFKVGPEPDLSVSYFYYPFE
                     SDADKKNSLLMKEIHADGDVFLSSSVIDGRYVIRMAILAFRTKRETIDRAITMILRCL
                     DKVRHVG"
     gene            complement(1206..2810)
                     /gene="nadB"
                     /locus_tag="PLG24_05505"
     CDS             complement(1206..2810)
                     /gene="nadB"
                     /locus_tag="PLG24_05505"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HMZ24380.1"
                     /translation="MAQTSDLVIVGSGIAGLSLAIQVAQRRKDLKITVLAKTTEKESN
                     TNFAQGGIATVWDVEVDNFEKHIADTLDAGDGLCDKEVVEIVVKSAPERVRELIDWGA
                     NFDKTQTGEYDLGREGGHTENRILHYKDITGREIQRTVVEKAAQFDNIEILEHYFAID
                     IITQHHLGYNVSRLTPNIECYGVYALDRKTNEIKTFLARQTVLASGGSGQVYQTTTNP
                     LVATGDGMAMFYRAKGRVCNMEFVQFHPTALYNPGGDKPDFLISEAVRGFGAILKDAQ
                     GREFMQKYDERLSLAPRDIVARAIDNEMKILGADKMFLDCRHLDKDGFLRHFPNIYEK
                     CKSIGIDPFVQMIPIVPACHYICGGIKIDHEGRSSINRLYAAGECTNSGLHGANRLAS
                     NSLLEGLVFGYRAAMDILATIDKYVICKGIPDWNARGTSDPKEMVLITQSRKEVNEIM
                     SNYVGIVRSNVRLKRADDRMYLLYKETEELYNTTTISPQLCELRNLITISYLIIKSAT
                     MRHESRGLHYTTDYPDKLPFLQDTLM"
     gene            complement(3278..4711)
                     /gene="pyk"
                     /locus_tag="PLG24_05510"
     CDS             complement(3278..4711)
                     /gene="pyk"
                     /locus_tag="PLG24_05510"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HMZ24381.1"
                     /translation="MADRSFQNTKIVATLGPASDSYETMVELVKAGVDVFRINMSHGS
                     HEVAARQIETISKLNKDYKINVGILADLQGPKLRVGQIENNVMELNAGDILTFVNEKC
                     VGNAEKIYMSYKNFAADVEVGEKVLIDDGKVGLEVIETNNVDTVRLKVLYGKELSSNK
                     GVNLPDTLVSLPAMTEKDLNDLEFLMTQPVNWIALSFVRGPKEVKALKDKIKQHKHHA
                     LVIAKIEKPQAVEKIEKIVKATDGIMIARGDLGVEMPIEKLPGIQKRIIALCVQFGKP
                     VIVATQMMDSMITNPSPTRAEVTDVANAVLEGTDAVMLSGETSVGAYPIETVLAMNRI
                     IAEAEKNYPKNHIRPYPSRKSETFMSDVVCFNAAKTAEELGASAIIGMTAMGYTGFKI
                     SSYRSPAEIFIFSDRKEIAGMLNLVWGVRCFHYDGFNSTDETITEVLQILKSKGRVRS
                     GETVVFVGSMPLKSKGMTNMMKVALVE"
     gene            complement(5051..5737)
                     /locus_tag="PLG24_05515"
     CDS             complement(5051..5737)
                     /locus_tag="PLG24_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ24382.1"
                     /translation="MVNKTVIVSGASGNLGTAVVNFYLDRGDQVIGLVHRSGGQPRNI
                     ANYVECAVNLLDELAVEECVKQLLEKFGKIDIAVMTAGGFAMGDLSDTGISELEHQYR
                     LNFETAYNLTKPLYEHMKLNHGGRLFFVGSGQGLDTSKGKHVVAYSLSKSLLFQLSNI
                     INADTAHTGIRAHVIVPSIIDTPRNRESMPDADFSKWEKPADIAAIIAKYADNHQSDK
                     QTIVVKDELK"
     gene            6368..>8156
                     /locus_tag="PLG24_05520"
     CDS             6368..>8156
                     /locus_tag="PLG24_05520"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HMZ24383.1"
                     /translation="MSENTEALQNNGNYDASNIQALEGLEAVRKRPGMYIGSTDTKGL
                     HHLVYEVIDNSIDEHLAGYCDRITTIIHPDNSISVTDNGRGIPVGMHEKLQKSALEVV
                     MTVLHAGGKFDKNTYKVSGGLHGVGVSCVNALSTYLKAEVHRNGHIYQQEYSEGKPTS
                     EVEVVGDAEDTGTIITFRPDAKIFETLEYKFETLSNRMRELAYLNKGLTLILIDERPD
                     ENGKTRNETYHSDGGLAEFVTYLDQSRTSIISKPIHVIGKEDHIEVEVALQYNTSYAE
                     NIYSYVNNINTREGGTHVRGFRMAVARLFKQYGEDNGFFKKLKFTVSGEDFREGLTAI
                     VSVKVPEPQFKGQTKGELGNSEVTSVVSQIVGETLRHFLEENPNEAKRILEKVVLAAT
                     AREAARKAREMVQRKNVLTGGGLPGKLADCSNRHPEESEIFLVEGDSAGGTAKQGRDR
                     HFQAILPLRGKILNVEKAMEHKIYENEEIKNMFIALGVSIEEKDGEKILNIEKLRYHK
                     IVIMTDADVDGSHITTLILTFFFRFMRPLIDNGYIYIATPPLYQVKKGQQAIYAWTET
                     DRKEAVAALSRKENDDSVKIQRYKGLGEMN"
BASE COUNT         2171 a         1867 c         1856 g         2262 t
ORIGIN      
        1 tgtcgaaacg ggcatcaatg cagcctccgg cacacatctg ggttatattc ccggtggcgg
       61 aatctttgct gctgcactgg cagatttcat cgccgcagta accaacccct atgcaggggt
      121 ctactatgct tcaccgggtg ccgtcactat cgaaaacgaa gtaatccact ggctgaaatc
      181 catattttcc tttcctgccg atgctgttgg caatctgacg tccgggggct ctgtctccat
      241 gctcattgcc ttcaatgccg cccgggataa atatggtgtg aagaatgaaa aagtcaggca
      301 aagcgtggtc tatttaagcc aacaagtgca ccattctgcc cagaaggcac ttcgaattct
      361 aggattggaa gatgtcatta tacgatatgt tccgctggac gacaggcaca ggatgaacgc
      421 agatatattg gcattgcttg ttgcggaaga caagtcaaga ggtctgcatc ctttccttgt
      481 cgttgctaca gcagggacta ccgatacagg tgcagtagat cctcttgatg caattgcgga
      541 tattactgaa atgcacaacc tgtggttcca tgtcgatggg gcatatgggg gattttttat
      601 cctcacttcc aagaaagaat tgttccgggg tattgaaagg gccaattcca tcattgtgga
      661 tccccataaa agtcttttcc tcccatatgg gcttggtgct gtcctaatcc gtgacaagga
      721 tgccgtgttg cactccaacc gttatgctgc caattacatg caagatgctc tgggtgacga
      781 acttgtcaaa agcccatcca acctgtcacc cgagctgacc aagcatttca gggggctgcg
      841 tatgtggttg ccattgcaac tccacggcct tgctcctttt atagcctgtc tggaggagaa
      901 acttctgctt acggcatact tcagggcgaa aatggtggca gtgggcttta aggtcgggcc
      961 ggagccggat ctttccgtca gttatttcta ttatccgttt gaatctgatg cagataagaa
     1021 gaacagcctg ttgatgaagg aaatccatgc tgatggagat gttttcctta gttcgtccgt
     1081 aatcgatggc cggtatgtta ttcgtatggc gattcttgcc ttccgtacca aacgtgaaac
     1141 gatagaccgt gcaataacaa tgatactccg atgtctcgat aaagtccggc atgtcggtta
     1201 agtgcttaca tcagggtatc ctgcaggaag ggcaacttat ccggatagtc agtcgtataa
     1261 tgcagtcccc gactctcatg cctcatcgtt gcactcttta taatgagata ggaaatggtg
     1321 atgaggttgc gcaactcaca aagctgaggg ctgatggttg tcgtgttgta aagctcttct
     1381 gtttccttgt acaacagata cattctgtcg tcggccctct tcagacggac atttgagcgt
     1441 acaataccca catagttaga catgatctcg ttgacctctt tacggctttg tgtaatgagg
     1501 accatttcct tcggatcgct ggttccgcgt gcattccagt ccggtattcc cttgcagatc
     1561 acatacttgt cgatggtggc gaggatatcc atcgctgcac ggtaaccaaa caccaggcct
     1621 tccagcaggc tgttgctggc gaggcggttg gctccatgca agccggaatt ggtacactca
     1681 ccggctgcgt aaagccgatt aatggagctt cttccctcat gatctatctt gattccgcca
     1741 catatatagt gacatgccgg tacaatgggt atcatctgga caaagggatc tatgccgatg
     1801 ctcttgcatt tttcatagat attggggaaa tgtcgtaaga aaccatcctt atccaggtgt
     1861 cgacaatcga ggaacatctt atccgccccc agaatcttca tttcgttgtc tatcgccctg
     1921 gcgactatat ctcttggtgc cagactcaac cgttcatcat acttttgcat aaattccctg
     1981 ccctgggcat ccttgaggat ggctccaaag ccccggacgg cttcacttat caggaagtcc
     2041 ggcttgtcac cgcccggatt gtacagcgca gtgggatgga actgcacaaa ttccatgtta
     2101 caaaccctgc cctttgcccg gtaaaacatg gccatcccat cgcctgttgc caccagggga
     2161 ttggtcgttg tctgatacac ctggcctgat ccgccggatg ccaatacggt ctgtctggcc
     2221 aaaaaggttt taatctcgtt tgtctttctg tccaatgcat agactccgta gcattcgata
     2281 ttgggcgtca ggcgggatac attgtatccg aggtggtgct gcgtgataat atcgatggcg
     2341 aaataatgct ccagaatctc tatgttgtca aattgggcag ctttctccac gacagtgcgc
     2401 tggatctcac gtccggtaat gtccttgtaa tggaggattc tgttttcggt gtgtccgcct
     2461 tccctgccaa gatcatattc tccggtttga gtcttatcga aattggctcc ccagtcgatc
     2521 aattcccgga ctctttcggg agcagatttt acaacaatct ccaccacttc cttgtcgcat
     2581 agaccatccc ctgcatccag ggtgtcggcg atatgttttt caaagttgtc cacctccaca
     2641 tcccatacag tagcaattcc accctgagca aagttggtat tgctttcttt ttcggttgtc
     2701 ttggcaagaa ccgtaatctt caggtccttg cgtcgctggg caacttgaat ggcgaggctg
     2761 agcccggcta tcccggagcc tacgatgacc agatcggaag tttgggccat gtcggtttga
     2821 tttttaaaaa aatgatgaca aagatacaaa tatagaagtt atggaaatac cgccaaaatt
     2881 caaccttttt agtcgccgct gtgtcgcaat taacgatttt tgcatcttga aacaacgccc
     2941 cattcgggct tttgtgatgc cggagtatcc gactgtaatg actccattcg aagttgacag
     3001 ataatagaag gcctcaaagg cttcgtgaag taggtagttt ttatttcagg ctgtactttg
     3061 tgttcaaata gagggcatgc ttttgtcaat atacagaatg aaatttttca tttcgatagc
     3121 gatattcatt atgcacatta tttcttaaat aggttcgata cttgaatggc aaatatttac
     3181 ctatattttt catgccactt ggcgaatccg gatcactcgc ctgccttgat ttttattttt
     3241 tggcagtaaa aaaaggatgg tatcaacaaa caatctacta ctccaccaaa gcgaccttca
     3301 tcatattggt catacctttg gactttaaag gcatggagcc aacaaacact accgtctcac
     3361 cactccgtac cctgcctttg gatttcagga tctgcaatac ttcggtaatg gtttcatccg
     3421 tgctattaaa gccgtcgtag tggaaacaac ggactcccca cacaaggttg agcatgccgg
     3481 ctatctcttt acgatccgaa aagatgaata tttctgccgg ggagcgatag gacgaaatct
     3541 tgaatcctgt atatcccatg gctgtcatac cgatgattgc agaggctccc agttcttctg
     3601 ctgttttagc ggcattgaaa cacacaacat ctgacatgaa ggtttctgac ttgcgcgaag
     3661 gataagggcg gatatgattc ttcggatagt tcttttcagc ttcggcaatg attctgttca
     3721 tggcgaggac tgtttcaatc ggataggcgc cgacactggt ctctccactc agcataacag
     3781 catcagtgcc ttcgagcacg gcattggcaa cgtccgtgac ttcagccctt gtcggtgacg
     3841 gattggtaat catactatcc atcatctgag tggccacgat gacaggcttt ccaaactgga
     3901 cacatagggc tatgatcctt ttttgtatgc ccggcagttt ttctatcggc atctctacac
     3961 cgaggtctcc ccttgcgatc atgatgccat ctgtcgcttt gactattttc tcaatttttt
     4021 cgactgcctg aggcttttca atcttggcaa tgacgagagc atgatgttta tgctgtttga
     4081 ttttatcttt taaggccttt acctctttag gccctcgtac gaatgataat gcaatccagt
     4141 tgaccggctg agtcatcaga aactccagat cattgagatc tttttctgtc atcgccggca
     4201 gggataccag tgtatcagga aggtttactc ccttattgga cgacaattcc ttaccataga
     4261 gaacctttag tcgtacagta tcgacattgt ttgtttctat cacttccagg cccactttgc
     4321 catcatcgat taatactttc tctcctactt cgacatctgc ggcgaagttt ttatacgaca
     4381 tgtatatctt ttctgcatta ccaacacatt tttcattgac aaaagtcagg atatccccgg
     4441 catttagctc catgacgttg ttctctatct gcccgacacg taatttaggg ccttgaagat
     4501 ctgcaaggat gccgacattg attttatagt ccttattcaa ttttgaaatc gtctcaatct
     4561 gccgggctgc aacttcgtga gaaccgtggg acatattaat cctgaataca tccactccgg
     4621 ctttaaccag ttctaccatg gtttcataag aatcacttgc cggtccgagt gtagctacta
     4681 ttttggtgtt ttgaaaactt cgatctgcca taagacaatg ataaatgatt taaggggaca
     4741 aaattagtgt actttttaca ataattactc aactcctgta agtatattat aaaaccaagg
     4801 gacatatcat ccctgaatgg gcttttccaa taacttatcg ggaagtaact atcctttaaa
     4861 aagcatcgag tcccgattct aattccatag aaataaaagt aaccccaagt atgagatgaa
     4921 aaaaatagca gccaattata caatccggga aaatgttgct ttaagtattt ggtaaaaata
     4981 cggtggagtg ctctcaaacc gccgggctat tcaatttcag gctgacgaat cagataaaaa
     5041 ttctaaactt ttactttaat tcatctttaa ccacgatggt ctgcttgtcg ctttgatgat
     5101 tatcggcgta tttagcaatg atggctgcga tatctgctgg cttttcccat ttgctgaaat
     5161 cggcatcagg catcgactct cgattccggg gggtatcaat gatgcttggg acgataacat
     5221 gggcccggat gccggtatgc gcagtatctg cattgatgat gttggaaagt tggaaaagca
     5281 gggatttgga aaggctgtag gctacaacat gtttaccctt cgatgtgtca agtccctggc
     5341 cggaacccac aaaaaacaac ctgcctccat gattgagctt catatgttca tacaaaggtt
     5401 tggttagatt gtaggctgtt tcgaaattaa ggcgatattg atgctccagc tcactgatgc
     5461 ctgtatcaga aaggtcgccc atggcaaatc ctcctgccgt cataacagca atatcgattt
     5521 tgccaaattt ttccaacagt tgctttacac attcctccac ggccaactcg tcgagtaggt
     5581 tgaccgcaca ctccacataa ttggcaatat ttctgggttg tcctcccgaa cggtggacca
     5641 ggccgattac ctgatccccc cgatccagat agaaattaac cactgccgtt cccagattgc
     5701 cggaggcgcc acttacaatc actgttttat ttaccatctt ttcgtaggag ctacttaaat
     5761 tccaactacg taaacaaaaa agaaggatta aagttatggc aatacaagag gaatcataac
     5821 ttttggccat tctccacgat ttgagttcga aaactgaact gggtagaaga ataataattg
     5881 accctccagc cggaatcagg gtctcaaaac atcccgaatg caataccatc catttaaacc
     5941 attattttgg aatttgtaat ccgggtgtgc ctgacatcat gctattccgg attattaatt
     6001 tcctacctac ttcatactgt gggaagatgc ggcagaattg accgatttta tagaaaaaat
     6061 gactgtgttg atggtccctc aatttttgta tatcctttaa ttaataacag accaacaatg
     6121 cctcgaacgg acttctaaat acactgtttc tattgctgac aagcgattgc tccggatgga
     6181 ttgacacgat ccggattttc ttccttgtgt aatctatgaa tcccgacaaa atttatccgg
     6241 ctcagaattt gattttttat ataaaatatc tcaaaatcaa tgtgatatat attttattca
     6301 ataaaacaat ccttggaatt cttgtaaaaa agtcatatct ttgtgctctt ttataaagag
     6361 aatttttatg tcagaaaata ctgaagcctt gcagaataat ggaaattacg atgcgagtaa
     6421 tatccaggca ctggaaggtc ttgaagcggt cagaaaaagg ccggggatgt atatcggaag
     6481 taccgacact aagggattac accatctggt atatgaagta atcgacaact cgatcgacga
     6541 gcatctggcc ggatattgtg acagaataac taccatcatt catccggaca attccatctc
     6601 tgtaactgat aatggacggg gaatacccgt gggaatgcat gaaaagctcc aaaaatcagc
     6661 tctcgaggtg gttatgaccg ttcttcacgc cggtggtaaa ttcgataaga atacctacaa
     6721 agtttccggc ggtcttcatg gtgtgggggt gtcgtgcgta aatgcccttt caacctacct
     6781 gaaagctgag gttcacagga atggccatat atatcagcaa gaatattctg aagggaagcc
     6841 aacctcggaa gtcgaagttg tgggcgatgc tgaggatacc ggaaccatca taactttcag
     6901 accggatgct aagatatttg agacgctgga atataagttt gagacgctgt ccaacaggat
     6961 gcgcgaactg gcctacctca acaaggggct cacgctaatt cttatcgatg aaaggccgga
     7021 tgaaaacggg aagacaagaa acgagacgta ccatagtgat ggtggccttg cagagttcgt
     7081 cacctatctg gatcagtccc ggacttccat catctccaag cccatccacg tcattgggaa
     7141 ggaggatcac atcgaggtcg aagtggcact acagtacaac acttcttatg ctgaaaacat
     7201 atattcctac gtcaacaaca tcaacaccag agaggggggg acacatgtaa gaggatttcg
     7261 catggccgta gccagattgt ttaagcaata tggggaggac aacggtttct tcaagaagtt
     7321 gaaatttacg gtttccggtg aggacttcag ggaaggtttg accgccatcg tgtcagtcaa
     7381 ggtgcccgag ccgcagttta aggggcagac caaaggggag ttgggcaact ctgaagtaac
     7441 cagtgtcgtc agtcagatag tcggggagac cttaagacac ttcctggaag agaatcccaa
     7501 tgaggccaag cgaatactgg agaaagtcgt acttgccgcc acagcgcgcg aagctgccag
     7561 aaaggcgaga gagatggtac aacgaaagaa tgtgctgacc ggagggggac tgcccggcaa
     7621 gctggcggac tgcagcaaca gacaccctga ggaaagtgaa atcttccttg tcgaggggga
     7681 ctccgcgggt ggtacggcga agcagggtag agaccgtcat tttcaggcca tcctgccctt
     7741 gagaggtaaa atcctcaatg tggaaaaagc aatggagcat aagatctacg aaaatgagga
     7801 gatcaagaac atgtttatcg ctctcggtgt aagtatcgag gaaaaggacg gtgagaaaat
     7861 cctaaacatc gaaaagctcc gatatcataa gatagtcatc atgacggatg ccgatgtcga
     7921 tgggtctcat attacgacat tgatactgac atttttcttc cgatttatga gaccattgat
     7981 tgataatggc tatatctata ttgcaacgcc accgctttat caggtcaaga aaggccagca
     8041 agcgatatat gcctggacag agacagacag gaaagaagca gtagcagcac tttccaggaa
     8101 agaaaacgat gacagtgtga agatacagcg ctataaaggt cttggagaga tgaatg
//
LOCUS       DAOQQN010000109         8155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3442_length_8155_cov_5.394444, whole genome shotgun sequence.
ACCESSION   DAOQQN010000109 DAOQQN010000000
VERSION     DAOQQN010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3442_length_8155_cov_5.394444"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="PLG24_05525"
     CDS             complement(<1..894)
                     /locus_tag="PLG24_05525"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMZ24384.1"
                     /translation="MCLFAQKGTLRGNVTDAVTGEPIMFGSVYIHQIGNGMMTDLDGN
                     YSIDLQPGNYDIKFTYIGYKDTVWNDVTLKSDDTITINCVMFPSSVVLDDIVVKAKHN
                     KSTTFALAAIKSKSANVMDGISASTFKAIGDGNAASAVGRVTGVSVQEGKYVYVRGLG
                     DRYSKTTLNRMEIPGLDPDRNTIQIDIFPTQVIDNILVSKSYTADLPADFTGGLIDIA
                     TKDFPNRKYMTFSFGVSYNPRMHFNQSFLSYKGGKTDFLGFDDGSRVLPVKPTERIPF
                     LSEAIANPAVDGKRYTGILESF"
     gene            complement(1015..2301)
                     /locus_tag="PLG24_05530"
     CDS             complement(1015..2301)
                     /locus_tag="PLG24_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24385.1"
                     /translation="MTNIKNLLFVFLACIPVLFTACDKDECIKTCAPNEVLTDNCECV
                     PVGDNSTVIVSGNISSDVTWTADKVYVLAQRVTVLPGVKLTIMPGTVIKGQAGSGANA
                     TALLIAKGAKIDAQGTVDKPIIFTSVADEIKSGEIVSPNLDQGINGLWGGVLILGDAP
                     ISANAPSVQIEGIPPSDINGLYGGNNPQDNSGIMKYVSIRHGGTNIGEGNEINGLTLA
                     GVGSGTIIENIEIVSNQDDGIEIFGGTVNPKNIMVWNTGDDMFDCDQGWSGTMDNIIG
                     IAGDQTDSALELDGGEGVENPTYTISNGTLKGHTAATSGKYADLRAKVSVSLKNLYFF
                     NFSEGSEFRLNDQGVSDSWFSGKILFANLEFNVSHMNTQPSLADIFRDKGSHDEDLNN
                     AAPGFAKISTANTVGARVSEFEKWTLVAALGQLAQL"
     gene            2812..2979
                     /locus_tag="PLG24_05535"
     CDS             2812..2979
                     /locus_tag="PLG24_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24386.1"
                     /translation="MVRMYGMGQFMDDYLVGSLKGCKEEPVGEYNVVLGYTGSPVRDI
                     ITEGVYPYLKF"
     gene            complement(3385..4245)
                     /locus_tag="PLG24_05540"
     CDS             complement(3385..4245)
                     /locus_tag="PLG24_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24387.1"
                     /translation="MKYILLYILCFGMVFGLHAQNTTWEQYDEELSTMGKIILTDTNS
                     TERKAAVEHVNDLFNTLLQDPATFKKKFVKSDFFSVLYPTDSSFRILTGQHFIKDDEY
                     RYYGGIQKASGGFIPFTDRSYENFSEGITNEELRADEWQGALYYRLFDCKLKGDKYYL
                     LLGYNAHSFFNKQKVIEVLSFDQDGRPLFGKDVFIRDSTDYGDDRLRYIYTYSADVAM
                     KLNYDNEEKMIVLDHLIPMREIFVGQGDTYVPDGTYEGFKYKRGRWRHISFLPQRFMK
                     ESEMKSGKKK"
     gene            complement(4433..5530)
                     /locus_tag="PLG24_05545"
     CDS             complement(4433..5530)
                     /locus_tag="PLG24_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMZ24388.1"
                     /translation="MFGWQFRKYDGEPELSLFDELLNLFKDLLMYAGGNVATAIQWLM
                     EMDREYNLTRDDYGIQDFLDELMQKGYLKADPEENALRPAEKMNISLRRKALKEVFGN
                     IKKATKGNHTTKKDGKGDELTSDLRPYVFGDSPEQIAFVESIKNAQINHGPGSFSLEY
                     EDLEIRETYHQSSMSSVLLIDISHSMILYGEDRITPAKKVAMALSEYISTRFPKDTLD
                     IVVFGDEAWKIEKDELPYLEVGPFHTNTLAALELALDILRKRKNTNKQVFMITDGKPT
                     CIKRGNKLYKNSFGLDRMIVNRTLNQAGKFRRLGIPITTFMIAKDPYLMEFVELFTET
                     NGGKAFYSDLQGLGDFVFKDYSRSRRWNNKW"
     gene            6111..6719
                     /locus_tag="PLG24_05550"
     CDS             6111..6719
                     /locus_tag="PLG24_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24389.1"
                     /translation="MVLMYQDHHTIQVIESDFGAFKIYLDDISPDTLYPRSSMEGMRI
                     KYTKSSKRIFINAPIQGITTTYIGKFRYRDSGDLERQKAAYDSIMRHKSKEVKTYGTY
                     YGTIFNSKTNKRQSDTIKVFDFKIDQTEGNFQVKYKMARIIFSGADSQPYEIRVEYNP
                     NNKTIRQSGKSSIKLFIDLENKVIRFEDKVKKIKFEGIRIEH"
     gene            6725..7666
                     /locus_tag="PLG24_05555"
     CDS             6725..7666
                     /locus_tag="PLG24_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24390.1"
                     /translation="MAGSVPYNIAILYYTQTGQLKMLAEKVSEGFSGDCRVDFIEIIP
                     VKQFPFPWNTIEFFDAMPECVLEEGSEIRLSGYEEGKHYDLVILAYQPWFLHPSLPIT
                     GFLQSKWADKLLKDVKVITLTGARNMWLNALEKIKRDLHKFGSEHVGNIAFVDSSPNL
                     ISTLTVIRWAFKGRKEASGLLPEAGVQVAEINRGPLFGKLIASALQEGKWQTLQQELV
                     KAGAVKLKPGLILLEETAIGQFKMWARKIKAKGERGSPARRPLVRLFKNILIVGIFIL
                     SPIKFVVSKIQAMLNRKRLVAAAKYFQSVNYEENKFS"
     gene            7682..8110
                     /locus_tag="PLG24_05560"
     CDS             7682..8110
                     /locus_tag="PLG24_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ24391.1"
                     /translation="MKKNEVPQDDEAIYQQKFGDGLLKYATDEQNEYVTVQSVGWEPE
                     IVALKQAWELVEEKVEMARQAVKNNQKSPIYYFMEKKLMDTGILAGYMGLWRWQVARH
                     FKPGVFARIKPSTLEKYAKVFDIDLKQLTDFDPDHPDTPK"
BASE COUNT         2505 a         1663 c         1624 g         2363 t
ORIGIN      
        1 aaaactttct aggattcccg tatatctttt accatctaca gccggattag caattgcttc
       61 tgacaaaaac ggaatccgtt ctgtcggctt tacgggtagg acccggctac cgtcatcaaa
      121 tcccagaaaa tctgtcttac cgcccttata actcagaaag ctctgattga aatgcatgcg
      181 tggattatag ctgacaccga aagagaaggt catgtatttt ctattcggaa aatccttggt
      241 agcgatgtct atcaatccgc ccgtaaagtc tgccggcaag tcagccgtat aagatttcga
      301 aaccagaata ttgtcaatca cctgagtggg aaagatatct atctgtatgg tatttctgtc
      361 gggatcaagt ccgggaattt ccattctgtt gagtgttgtt ttggaatatc ggtcacccag
      421 tccgcgaacg tacacatact tgccttcctg aaccgaaaca cccgtcacac ggcccacggc
      481 agaggctgca ttgccgtcac cgatagcctt gaatgtagag gctgaaatac cgtccatcac
      541 attggctgat ttacttttta tggctgccag ggcaaaggtt gtactcttgt tgtgttttgc
      601 cttgactaca atatcatcca gaaccacgct ggacggaaac attacacagt tgatcgtaat
      661 ggtgtcatcc gattttaaag tgacatcgtt ccatacggta tctttataac cgatataggt
      721 aaacttaata tcgtaattac ccggctgaag atcaatggaa taattaccgt caaggtccgt
      781 catcatgccg ttgcctattt gatgaatgta aaccgagcca aacataatag gttctccggt
      841 cactgcatcc gttacattac ctctcagggt gcctttctga gcgaaaagac acataggaag
      901 cagaattagt atacataaat tataaatact tttcactata aaaaaatttt aagaacacat
      961 tccctgcact gtgaaaggag ggaatgtgtg catgaaaaaa aaataatatt gtaattacag
     1021 ttgagcgagc tgacccagag ctgctaccag ggtccatttt tcaaattcac ttaccctggc
     1081 accaacggta ttagctgtgc taattttagc gaatccggga gcggcattgt tcagatcctc
     1141 atcgtgagaa cccttatccc ggaaaatgtc agccagggaa ggttgtgtat tcatgtggct
     1201 gacattaaac tccaggttag cgaaaagtat ttttcctgaa aaccatgagt ctgagactcc
     1261 ctggtcattt agcctgaatt ctgaaccttc agaaaaatta aagaaatata aatttttcaa
     1321 agaaacagag accttggctc ttaggtcagc atacttacct gaggtggcgg cagtatgtcc
     1381 ctttaatgtg ccgtttgaga tggtgtatgt aggattttca acaccttcac ctccatccag
     1441 ttcaagagcg ctgtcggtct ggtcacctgc aattccgatg atgttgtcca tggtcccgga
     1501 ccatccctga tcgcagtcaa acatatcatc gcctgtattc cagaccataa tattcttagg
     1561 gttgaccgta cctccgaata tttcgatgcc atcatcctga ttggaaacta tttcgatgtt
     1621 ttcgatgatg gtgcctgatc ctacaccagc cagcgtcagg ccgttgattt cattaccttc
     1681 gccgatgttg gttccaccgt gacggattga aacatatttc attatccctg aattgtcctg
     1741 cgggttattg ccaccataaa ggccattaat gtcggaaggt ggaatacctt caatctgaac
     1801 agaaggcgca ttggctgata taggagcatc tccgaggatg agcactccgc cccagaggcc
     1861 attgattccc tgatccagat tagggcttac gatttcgccg gattttattt catcggcaac
     1921 agaagtgaag atgatgggct tgtcaacggt accctgagcg tcaatcttcg cccctttggc
     1981 aattaggagg gcagtagcat tggctccact tcctgcctga cctttaataa cagttccggg
     2041 catgatggtc aatttgacgc cgggtaaaac agttactctc tgtgccagga catagacctt
     2101 atcagctgtc caggtcacat cagacgaaat atttcccgag acgattacag tactattgtc
     2161 gcccacaggg acacactcac agttatcggt cagtacttca ttgggggcac aggttttgat
     2221 acattcatct ttatcacatg ctgtaaataa aaccgggata catgcaagaa atacgaataa
     2281 cagattttta atgtttgtca tataaattca tttttggtta gacattttct tcaaatattc
     2341 cgcaaaaatg aatcggcaac attaaggaaa tattaattct atatgacgat attgttaagt
     2401 cctccaaatg gttaatatta agtcttcaga ggaaaagaat cattgaaatc cggtgaaata
     2461 tggagtgaga aaacaaaata aagtgtttga tggatgaatt tattatccaa aggaatgatg
     2521 ggtgggatgt gaattaagga tttatggaaa taagtggtga taaaggagac ttgttgtatt
     2581 tccagatagc caggccatac gggaaatgac aagggattcg acaccatggt aggcattgta
     2641 aaaaggtgtc ctccaggtta tcttttacac gaggattcat taaagtgcgg ttgaaattca
     2701 ggtattcatt ttgaattaaa acactaaggt atttccgtat tgttttgagc cgggatggtg
     2761 gtgcctcatg tcgggatcgt gctgtagcac cagtcgccgg aatttgaatg aatggtccgt
     2821 atgtacggta tgggccagtt catggatgat tacctggtcg ggagtctcaa ggggtgcaag
     2881 gaggagccgg ttggagagta taatgttgtt ttgggatata caggatcccc ggtgagagac
     2941 atcattacgg agggtgtata tccgtatcta aaattttaac tcaagttgtc ttttaatcaa
     3001 caaattttat taaaaaacga taaaaaattg gcaaatctca acttgaaaag ggacatcgct
     3061 ctgtatgaaa aatcacaaat tacagcagtt caatcgatgt ttggttaccg gatttccacg
     3121 ggaaatcgga tgttataaga aataagaaat atctgttcgc caataaatct gtagtacaca
     3181 aagccgagca ccttctcccg gaaagtccca aactgtgcag aatatcattt gcctatgcgc
     3241 aattgggtta actaattgcc ctatctattc aaaggcaatt caaaacctaa ataaaatatt
     3301 tatctgatac cacgaaagag caaaattcac agggtgaaat tatagaagct tcaaacagct
     3361 atgtatttat acactccaaa gacctcattt ctttttccct gacttcattt cgctctcttt
     3421 catgaatctt tgagggagga atgaaatatg gcgccatctg cctcttttat atttaaagcc
     3481 ttcataggtt ccatccggca catacgtatc gccctgacca acaaagattt ctcgcattgg
     3541 tatcagatga tccaggacaa tcattttttc ttcattgtca taattaagtt tcatagcgac
     3601 gtcagccgaa taggtataaa tatatctaag ccgatcatcc ccataatcgg ttgaatctct
     3661 gataaataca tccttaccaa ataagggccg tccatcctgg tcgaaagaaa gtacttcaat
     3721 gaccttctgc ttgttgaaaa aagaatgagc attgtatcca agtaataaat aatatttatc
     3781 ccccttcaat ttacaatcaa aaagccgata atacaaggct ccttgccatt catcggccct
     3841 cagttcttca ttagtaattc cttcggaaaa attctcatag gaccgatccg taaatgggat
     3901 aaaacctccc gaagcctttt gtattccacc ataataacga tattcgtcat ccttgataaa
     3961 atgttggccg gtcaatatcc tgaaagatga atctgtcgga taaagcactg aaaaaaaatc
     4021 tgacttgacg aatttcttct taaaagttgc agggtcttga agcaatgtat tgaataagtc
     4081 attgacgtgt tctacagcgg cctttctttc tgttgaatta gtatccgtaa ggattatttt
     4141 acccattgta ctaagttctt catcatattg ctcccaggtt gtattttggg catgcaaacc
     4201 gaaaaccatt ccaaagcata gaatgtataa caggatatat ttcacgtcag atttaattaa
     4261 ttgagggtta agttaatcaa gttttaagtt gtattgcgat tggcattaat agaaaccttt
     4321 gtaaaattat tcaatcatcg tccataacgt caattcccag gcaatccata cttataaatt
     4381 tgttaattca ttttattagg gtaaaattaa taacaatgct gaacaaagaa tttcaccatt
     4441 tgttattcca tcttcttgat ctactgtaat ccttgaagac aaaatcacct aatccttgca
     4501 agtcactata aaatgccttg cctccattcg tttcggtaaa aagttcaaca aattccatca
     4561 aatagggatc tttagcaatc atgaaggtag tgataggtat gccaagccgt ctaaacttgc
     4621 cggcttgatt gagggtacgg ttgacaatca tcctgtcgag accaaagcta tttttgtaca
     4681 gcttattacc tcgtttgata caagtcggct ttccatctgt aatcatgaag acctgtttgt
     4741 tggtattttt tctctttcgg agaatgtcca gtgccaattc aagtgcagcc aatgtattgg
     4801 tatgaaacgg gcctacctcc agatagggta attcatcctt ttcgatcttc caggcctcat
     4861 ccccgaacac aacaatatca agagtatcct ttggaaatct ggtcgatata tattccgaca
     4921 aagccatggc tacctttttg gccggagtaa tcctgtcttc accatacaat atcatcgagt
     4981 gcgaaatatc gataagtaag acgctactca tactactctg gtgataggtt tcacggattt
     5041 ccagatcctc atactcaagg ctgaaagagc ccggtccatg gttgatctgt gcattcttta
     5101 tcgattcgac aaatgcgatc tgttcagggc tgtctccgaa tacatatggc cgcaagtctg
     5161 aagtcaattc atcacccttg ccatcctttt ttgtagtatg attgccttta gtggctttct
     5221 ttatgttgcc aaaaacttcc ttcaacgctt tacgacgtaa ggaaatattc atcttctctg
     5281 caggtctcaa agcattttcc tcgggatcgg ctttcagata acctttttgc atcaattcgt
     5341 ctaaaaaatc ctggataccg tagtcatccc tcgtaagatt atactcccga tccatctcca
     5401 tcaaccattg aatggcggtc gctacattac ctccggcata catcaataga tccttaaaca
     5461 ggttgagcag ctcatcaaaa agagacaact ccggttcacc atcgtatttt ctaaattgcc
     5521 aaccaaacat tttccaatat ttgaattatt aaattcccct tgtcaacatc cacaatccat
     5581 tgtggttata ttattctact ttaccttgtt cgtggacatc tttacatcaa acttaaaaga
     5641 atacaacctt catgcgctgt caaatacatt tttaaagaaa ataaaatttc atgtagataa
     5701 aatcaaacaa atacaacaat tataaaacgc ttttcgtgct tgtcaaatag tttaaaagct
     5761 taccaataac catactttta ctcttcagat aaaatgtata accgaaaatg gattactttt
     5821 gttgctgttc gggaaacttg taccaaattc aattgtaaaa caaattatac cgtaactttg
     5881 ttagtgtcgg aattgatttt ttcaatagtt tcaccatcaa agttgtatac tgtcggaaat
     5941 aaattatgtg ccaaaaaata aaatatcttt ttgtccccgt ctgggtatta atctcatcct
     6001 cattactttt tgcgcaagat aaaaatcatg atgaatttgt aaaaagtatt tgcggaacgt
     6061 attccgggaa ttacatcacc aaaaaaggag ttcctaaaaa cggagccgag atggtactta
     6121 tgtatcagga ccatcacact atccaagtaa tcgaatccga cttcggagca ttcaaaatat
     6181 atttggacga tatctctccg gacaccttgt atccaaggtc gagtatggaa gggatgcgga
     6241 ttaaatatac caaaagttca aaaaggatat ttatcaatgc tcccatccaa ggcataacta
     6301 cgacctatat cggcaagttc aggtacaggg attcaggtga tcttgaaagg caaaaggcgg
     6361 catacgattc aattatgcgc cataagtcga aggaagtcaa gacctatggt acttactacg
     6421 gtacaatttt caattccaag actaacaaaa gacaatcaga tacgattaag gtgtttgatt
     6481 ttaagataga tcagacagaa ggaaattttc aagtaaagta taagatggcc agaatcatat
     6541 tttccggtgc cgattctcaa ccttacgaga tccgggtgga atataatccc aataataaga
     6601 ccatcaggca atccggcaaa tcaagtatta agctctttat agatcttgaa aacaaagtaa
     6661 ttcgtttcga agacaaggta aagaaaataa aattcgaagg catcagaatc gaacattgaa
     6721 attcatggca ggatccgttc cctataatat tgctattctt tattacactc agaccgggca
     6781 gttgaagatg cttgcggaaa aggtatcaga aggattttct ggtgattgtc gggttgattt
     6841 cattgagatt atcccggtta agcaatttcc ctttccatgg aatacaatag aattttttga
     6901 tgctatgcct gaatgcgtat tggaagaagg ctcagaaata agactatccg gttatgaaga
     6961 ggggaagcat tacgatcttg tcattttggc ctatcagcca tggtttctgc atccatctct
     7021 ccctataacc ggttttcttc aaagtaaatg ggctgacaaa ctcttgaagg atgtcaaggt
     7081 cattactctt accggagcaa ggaatatgtg gttaaatgcc ttagaaaaga taaaacgcga
     7141 cttgcataag ttcggatcgg agcatgtagg caacattgca tttgtagatt catcacccaa
     7201 tctcatctcc acgctgactg ttattcgttg ggcgttcaaa ggccgcaagg aagcgtccgg
     7261 attgctaccg gaagcgggtg tacaggtagc tgaaatcaac agaggtcctt tgtttggcaa
     7321 attgattgcc agtgccttac aagaaggcaa atggcaaact cttcaacagg aattggtcaa
     7381 agccggggca gtaaaattga aacccggtct gatcctgctg gaagaaacgg caattggcca
     7441 gtttaaaatg tgggcccgga aaatcaaagc aaaaggtgag cgcggatcac ctgcccgaag
     7501 gcccttggtc agattattca agaacatact gattgtgggg atttttatcc tttcgcccat
     7561 taaatttgta gtttcgaaga tacaagccat gctcaaccgg aaaaggctcg ttgctgcggc
     7621 aaaatatttt caatccgtaa attatgaaga aaataaattt tcctgataac tagtcaaagc
     7681 tatgaagaaa aacgaagtgc ctcaagacga cgaagctatc taccagcaaa aattcggaga
     7741 cggactgctg aaatatgcca ctgacgaaca aaatgaatat gtcacggtgc aatcggttgg
     7801 ctgggagcct gagatagtag cattaaaaca agcctgggaa ttggtggagg aaaaggtgga
     7861 gatggcaaga caagcagtga agaataatca aaaaagtccg atctattatt tcatggaaaa
     7921 gaaattgatg gatacgggaa ttttggcagg atatatggga ttatggcgat ggcaggtggc
     7981 aaggcatttt aagccgggtg tatttgcaag aataaaacct tcaaccctgg aaaaatacgc
     8041 caaagtattt gatattgacc taaaacaatt aacggatttc gacccggatc atcctgacac
     8101 acccaaataa agcatgaaaa aaaattgatg aacttacaac atatacaagc tgcac
//
LOCUS       DAOQQN010000110         8119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3468_length_8119_cov_4.999876, whole genome shotgun sequence.
ACCESSION   DAOQQN010000110 DAOQQN010000000
VERSION     DAOQQN010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8119
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3468_length_8119_cov_4.999876"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PLG24_05565"
     CDS             complement(<1..282)
                     /locus_tag="PLG24_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24392.1"
                     /translation="MSLKKKNSTPKQNFSSITIRLASPDEILERSYGEVLKPETINYR
                     SYKPERDGLFCERIFGPVKDYECYCGKYKRIRYKGIVCDRCGVEVTEKKV"
     gene            complement(348..4169)
                     /gene="rpoB"
                     /locus_tag="PLG24_05570"
     CDS             complement(348..4169)
                     /gene="rpoB"
                     /locus_tag="PLG24_05570"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HMZ24393.1"
                     /translation="MSSNIASLNSELKRHNFGKIKLSDHQPDLLEIQLKSFKEFFQLE
                     TTPENRINEGLYRVFQENFPISDARNIFVLEFLDYYIDPPRYDIDECMQRGLTYSLPL
                     KAKLRLSCNDEEHVDFQTIVQDVFLGNIPYMTPKGTFVINGAQRVVVSQLHRSPGVFF
                     SQTFHPNGTKIYSARVIPFKGAWMEFATDINTVMYAYIDRKKKFPVTTLLRAIGFDSD
                     KDILEIFGLSEEIPAKKKDLQKAVGRKLAARVLRKWTEDFVDEDTGEVVTIERNEIIL
                     ERDVILEEAHFDLILDAGIDTVILQKEDIEEDYTIIYNTLQKDPASSEIEAVEYIYRQ
                     LRGAEPPDEETARSIIDKLFFSDKRYNLGEVGRYKVNKKLGLSTDPNTMVLTKEDIIM
                     IVKYLVSLMNQKAELDDIDHLSNRRVRTVGEQLFAQFGIGLARMARTIRERMNVRDNE
                     VFTPVDLINARTLSSVINSFFGTSQLSQFLDQTNPLSEITHKRRISALGPGGLSRERA
                     GFEVRDVHYSHYGRLCTIETPEGPNIGLISTLCVHAKINKMGFLETPYRKVAKGKVDL
                     KSEVEYLSAEAEDYKRIAQANTPIDDKGDILTERIKSRDQGEFPVLEPSEVEYIDVAP
                     NQIVGISASLIPFLENDDANRALMGSNMQRQAVPLIRPSSPIVGTGLEGKVARDSRML
                     INAEGNGVVDYVDASKIVIRYDRSEEDQLVSFEDNVKTYVLTKFLRTNQGTCINLKPI
                     VKKGMRVEAGTVLCEGYATENGELALGQNLKVAFMPWKGYNFEDAIVLSERVVREDIF
                     SSIHIEHFELDVRDTKLGEEELTPDIPNVSEEATKDLDDNGIIRIGAWVNEGDILIGK
                     ITPKGESDPTPEEKLLRAIFGDKAGDVKDASLKCPPSIEGVVIDKQLFARAKKDKVQK
                     VQEKDLLDRLEDDHQVVLNELKTTLIDKLMVLVKNKVTNGIKSIYGEEMLAKGSKFNK
                     TVLKAIDYNTVDYANWTDDDAVNSQIVRMLHNYSIKVNEELGHYKREKFNISIGDELP
                     AGVLKLAKVYIAKKRKLKVGDKLAGRHGNKGIVSRIVRVEDMPFLEDGTPVDIVLNPL
                     GVPSRMNLGQIFETVLGWAGDKLNIKYATPIFDGANLDEIAVELNKAGLPELGQTYLY
                     DGETGDRFHQKATVGVIYMLKLSHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFG
                     EMEVWALEAYGASNILQELLTIKSDDIQGRAKAYEAIVKGDNLPEPNIPESFNVLVHE
                     LRGLALDVGFD"
     gene            complement(4401..4784)
                     /gene="rplL"
                     /locus_tag="PLG24_05575"
     CDS             complement(4401..4784)
                     /gene="rplL"
                     /locus_tag="PLG24_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HMZ24394.1"
                     /translation="MADLKSFAEQLVNLTVKEVNELAAILKEEYGIEPAAAAVAVAGP
                     VAGGGGGEAAAAVQTEFDVILKSAGANKLAVVKEVKNLLNLGLKEAKDIVDAAPAPLK
                     NGVSKDEADSIKAALDAAGAEVEIK"
     gene            complement(4882..5409)
                     /gene="rplJ"
                     /locus_tag="PLG24_05580"
     CDS             complement(4882..5409)
                     /gene="rplJ"
                     /locus_tag="PLG24_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HMZ24395.1"
                     /translation="MNKAQKTQVIEDLKEKFANATFFYITDASTLTVAQVNSFRKLCF
                     EQNVQMQVVKNTLVKKAFEASDESKNFSGLYDSLHGPTALLFCETANIPAKILEKFRK
                     ANSKPILKAAYIDSSVYIGDDQIEVLTKLKSKEELVGEIIGLLQSPAKNVISALKSGG
                     STIAGLLKTLEERAN"
     gene            complement(5431..6123)
                     /gene="rplA"
                     /locus_tag="PLG24_05585"
     CDS             complement(5431..6123)
                     /gene="rplA"
                     /locus_tag="PLG24_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HMZ24396.1"
                     /translation="MSKLTKKQKASQDKIDANKFYPIEEAMAVVKDANYAKFDASVDV
                     HIKLGVDPRKADQAIRGTVSLPHGTGKTKTVAVFCTPEKEDEAKAAGAEYVGLDELID
                     KVAAGWTDVDVVVAMPGVMAKMGKIARILGPRGLMPNPKTGTVTNEVGAAVSEVKKGK
                     ISFRVDKYGIVHSSVGRISFSPSQLADNANELLQTLNKMKPSSAKGTYMKSITVASTM
                     SPGVKIDTRSIK"
     gene            complement(6218..6661)
                     /gene="rplK"
                     /locus_tag="PLG24_05590"
     CDS             complement(6218..6661)
                     /gene="rplK"
                     /locus_tag="PLG24_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HMZ24397.1"
                     /translation="MAKEVEYKIKLQVKGGQANPAPPVGPALGAKGVNIMEFCKRFNA
                     ETQDKMGKVCPVLITVFKDKSFEFVVKTAPASVQLMELSGAKKGSAESNRNKVGKVTW
                     DQVRAIAEDKMPDLNAFTVESAMKMVAGTARSLGITVEGTAPWDN"
     gene            complement(6710..7279)
                     /gene="nusG"
                     /locus_tag="PLG24_05595"
     CDS             complement(6710..7279)
                     /gene="nusG"
                     /locus_tag="PLG24_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HMZ24398.1"
                     /translation="MSETPEITVPSDRKWYSLRVISGKEKKAKEAIDLELLRSGWKDI
                     ITQVVVPTEKVYKIEKGKKVIRERNILGGYILIEAVFKKMTGEIIQTINNIPNVIHFL
                     GGQKPIPMQDAEANRLLGKIDESQDMDEAMLEPFIVGETVKIIDGPFNEFVGDIKEVN
                     DEKKKLKVIVKIFGRGTEVELNFVQVEKK"
     gene            complement(7283..7474)
                     /gene="secE"
                     /locus_tag="PLG24_05600"
     CDS             complement(7283..7474)
                     /gene="secE"
                     /locus_tag="PLG24_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HMZ24399.1"
                     /translation="MDKITSYLKESYDELVHKVEWPTGVMLVQSTVATIVGLLILSLL
                     VLGMDGLNKTLFDFIYKIK"
     gene            complement(7489..7561)
                     /locus_tag="PLG24_05605"
     tRNA            complement(7489..7561)
                     /locus_tag="PLG24_05605"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7526..7528),aa:Trp,seq:cca)
     gene            complement(7631..>8119)
                     /locus_tag="PLG24_05610"
     CDS             complement(7631..>8119)
                     /locus_tag="PLG24_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HMZ24400.1"
                     /translation="INVNNPVEIIGMMKEGEKPLTSTVTGVEMFRKLLDRGEAGDNAG
                     LLLRGIDKTQIKRGQVICAPGSVKPHKKFKCEVYVLSKEEGGRHTPFFNGYRPQFYFR
                     TTDVTGDCQLPADVKMVMPGDNVSLDVTLLSTIAMEKGLRFAIREGGRTVGAGQVTEI
                     IE"
BASE COUNT         2142 a         1921 c         1505 g         2551 t
ORIGIN      
        1 tactttcttt tcagtcacct cgacaccgca gcgatcgcag acgatgccct tatatctgat
       61 tcttttgtat ttgccacaat agcattcata atcctttacc ggaccaaaga ttctttcaca
      121 aaaaaggccg tctctttcag gcttatatga gcggtaattg attgtctctg gttttagaac
      181 ctcgccatat gaccgctcta aaatctcatc cgggcttgcc aaccgaatgg taatggaaga
      241 gaaattttgc tttggtgttg aatttttttt ctttaaagac ataatttata taaaaattat
      301 cggattctta aaaatggctg tctatgggga tttaacgatt caattcatca gtcaaatcca
      361 acatcaagag ccaatcctct caattcatgt accaatacat tgaatgactc aggtatatta
      421 ggctccggca ggttatcacc ttttacaatg gcttcataag ccttagccct tccctggata
      481 tcatccgact ttatggtcaa taattcctgt aagatattag aagcgccata tgcttcaagt
      541 gcccatacct ccatctctcc aaatcgctgc ccaccaaact gagccttacc tccgagtggt
      601 tgttgtgtga tgagagagta tggcccgata cttcttgcgt gcatcttgtc atcgaccatg
      661 tgcgatagct taagcatata gatgacacct actgtagctt tctggtggaa tctgtctccg
      721 gtctcaccgt cataaagata ggtctgaccc aattcaggca atccagcttt attcaactct
      781 acagcaattt catcgaggtt agcaccatcg aagataggtg ttgcgtactt aatattcaat
      841 ttatcgccgg cccagcctaa tacagtttca aaaatctgac ccaggttcat ccttgatggt
      901 acaccaagtg ggtttagtac gatatcgacc ggtgtaccat cttcgaggaa tggcatgtct
      961 tcgactcgca cgattctaga tacgattcct ttgtttccgt gacgacctgc gagcttatcc
     1021 cccaccttca gctttctctt tttggcgata taaacttttg caagtttcaa tactccagca
     1081 ggcaattcat ctccgatgct gatattgaac ttctcacgct tatagtggcc taattcctca
     1141 ttgaccttta tactgtagtt gtgcaacatc ctgacaatct gactgttgac ggcatcgtca
     1201 tcggtccaat tggcataatc cactgtatta tagtcaattg ccttaagtac tgtcttattg
     1261 aacttggatc ccttggccaa catttcttct ccgtagatgg atttgatgcc attggtcact
     1321 ttatttttaa caagtaccat aagtttatcg atcagagtag tcttcaattc attgagaact
     1381 acctgatggt catcctccag tctatccaga agatcttttt cctgaacttt ctgtaccttg
     1441 tccttcttgg cacgtgcaaa caattgctta tcgatcacca ctccctcaat agaaggcggg
     1501 catttcagtg aagcgtcctt gacatctccg gctttatcac cgaagatggc ccttaataat
     1561 ttctcttcag gcgttgggtc actttctcct tttggtgtga tctttccaat cagaatatct
     1621 ccttcattaa cccaggcacc gattcttatt atgccattgt catccagatc cttagtggct
     1681 tcttcgctga cgtttggtat atccggtgtg agttcttcct caccaagctt agtatctcgt
     1741 acatcaagct caaagtgctc aatatgaatg gatgaaaaga tatcttctct tactacacgc
     1801 tccgacagaa cgatagcatc ctcaaagtta taacccttcc agggcatgaa ggctactttc
     1861 aggttttgtc ccagtgccag ctcgccattt tctgtagcat atccttcaca caacactgtt
     1921 ccggcctcca cacgcatacc cttcttaaca ataggtttca ggttgatgca tgtaccctgg
     1981 ttggtgcgga ggaattttgt caatacatat gttttgacat tatcttcaaa ggagaccaat
     2041 tggtcttcct cacttctgtc atagcgaatc acgatcttgg aggcatcgac ataatcaact
     2101 acaccgttac cttccgcatt gatcaacatc cttgagtctc tggctacttt accttcaagg
     2161 cctgttccaa ctatcggaga ggacggccta atcaaaggca ctgcctgacg ttgcatgttt
     2221 gatcccatca atgccctgtt cgcatcatca ttctcgagga atggaatgag cgatgctgag
     2281 ataccaacga tctggtttgg tgccacatcg atgtactcca cctcacttgg ttccaataca
     2341 gggaattctc cctgatctct ggatttgatc ctttcagtaa gaatatctcc cttatcatca
     2401 ataggcgtat tagcctgcgc aattctttta taatcttctg cttcagcaga caaatattca
     2461 acttcagatt taagatcaac ctttcctttt gccacctttc tgtatggtgt ttcaaggaag
     2521 cccatcttat taatctttgc atgtacgcaa agggtagaaa tcaatccaat gttcggtcct
     2581 tccggtgtct caatggtaca aagacgtcca taatgcgaat agtgaacgtc acgcacctcg
     2641 aagccggctc tttccctgga aagaccaccc ggtcctaagg cagaaatcct tcttttatgc
     2701 gtaatctcag aaagcgggtt ggtctgatca agaaactgag aaagttgtga agtaccgaag
     2761 aaagaattga taactgaaga taatgttctc gcattgatca ggtcaaccgg agtaaatact
     2821 tcattatcgc gtacattcat tctttctctg atggttcggg ccattctggc caatccgata
     2881 ccgaattgag caaacaactg ctcccctaca gttctaacac gcctgttgct caagtgatca
     2941 atatcatcga gttcagcttt ctggttcatc aatgaaacca gatacttaac gatcatgatg
     3001 atatcctcct ttgtcaacac catcgtatta ggatcagtag agagacccag ttttttgtta
     3061 accttgtaac gtcctacttc acccagatta tatcgtttat ctgaaaagaa caacttatcg
     3121 atgatgcttc tggctgtctc ctcgtctggt ggctcagcgc cacggagctg gcggtagata
     3181 tactcgacag cttcaatttc actacttgcc gggtccttct ggagtgtgtt gtatatgatg
     3241 gtgtaatctt cctcaatgtc ctctttctgc aagatcacag tatcgattcc tgcatcaaga
     3301 attagatcaa agtgcgcctc ttccaggata acgtctcttt cgaggatgat ttcatttctt
     3361 tcgatggtga caacttcgcc ggtatcttca tcgacaaagt cttcggtcca ttttctaagg
     3421 acacgtgcgg ccaattttcg gccgactgct ttctgcaaat ccttcttttt tgccgggatc
     3481 tcttccgaaa gaccaaaaat ctccaggata tctttatcgg agtcaaaacc gatggctcta
     3541 agaagtgtgg ttaccgggaa tttcttttta cggtcaatgt aggcgtacat caccgtattg
     3601 atatcggtgg caaactccat ccaggcacct ttaaagggga ttacccttgc cgaataaatc
     3661 ttcgtaccat tcgggtgaaa agtttgacta aaaaacacac cgggagagcg atgcagctgc
     3721 gatactacaa cgcgttgagc accattaatt acaaaggtac cctttggtgt catataagga
     3781 atattgccca gaaacacatc ctgaacaata gtctggaagt caacatgttc ttcgtcatta
     3841 caggacaaac gtaacttagc cttaaggggg aggctatagg tcaatcccct ttgcatacat
     3901 tcgtcgatat cgtatcttgg tggatcaatg tagtaatcca gaaactccaa aacgaagata
     3961 tttctggcat cgctgattgg gaaattttcc tgaaacactc tatacaaacc ttcattgata
     4021 cggttttcag gagtggtttc cagctgaaaa aattctttaa aagactttag ctgaatttcg
     4081 agtaagtcag gctgatggtc tgaaagtttg atttttccaa aattatgtct tttcaactcg
     4141 ctattgagag aggctatgtt tgagctcatt agatcgatct gttttagaat ataatgttat
     4201 ccgggataac ctgggataga tgaaatcaag ataattggga cgtcattaag tagttttaat
     4261 tcatatccac ttataggcat aggcttagca agactgtgca cagtcctact aagcctaatc
     4321 aatatagtaa atcactttat tcagcaacac tatgtatgta cccaactgca ccgaaatgct
     4381 gttggctatg gaattaaata ttacttaatc tctacctcag ctcctgcagc atccaaagcc
     4441 gctttgatgg agtctgcctc atccttagaa acaccattct tcaatggagc aggtgcagca
     4501 tcaactatgt ccttagcttc tttcaagccc aggttcaaca agtttttcac ttctttaacg
     4561 actgcaagtt tgtttgcacc tgctgacttc aggataacat caaattcagt ttgaactgct
     4621 gctgctgctt caccaccgcc accacctgct acaggtccgg caactgctac tgctgctgca
     4681 gctggctcga tgccatattc ctctttgagg atagctgcta attcattaac ctctttaaca
     4741 gtaaggttaa ctaattgttc tgcgaaagat tttaaatccg ccattgtaat aaatttttaa
     4801 attttagtca ataaatatat tattgcgtaa agcttggaag ccgtattcaa taggtggcat
     4861 catgatgcct ttggaaaaag atcaattcgc tctttcttcc aaagttttga gtaaccctgc
     4921 gatggtagaa cctccggatt tgagtgcgga gataacattt ttcgcaggag actgcaacaa
     4981 tccgatgatt tcgccaacca attcttcctt ggatttcagc ttagtcagaa cttcaatctg
     5041 atcatcgcca atatagacgg aagaatcaat atatgcggcc tttaggattg gtttgctatt
     5101 ggcttttctg aatttctcca gaatcttagc aggaatattt gcagtctcgc agaaaagcaa
     5161 tgctgtaggc ccatgaagtg aatcatacaa accggaaaag ttctttgact catcagacgc
     5221 ctcgaatgct ttcttcacca atgtattctt tactacctgc atctgaacat tctgttcaaa
     5281 gcatagcttt ctgaagctat ttacctgagc aaccgtcagt gtagaagcat cagtgatgta
     5341 aaagaaagtc gcattggcga atttctcttt caggtcttca attacctgtg tcttttgtgc
     5401 tttattcata tctgtcagtg ttgatagtaa ttatttaata gatcgggtat caattttcac
     5461 gcccgggctc atggttgaag cgacagtgat cgacttcata taagttcctt ttgcggaaga
     5521 aggcttcatt ttattgagtg tttgcagtaa ttcattggca ttatcagcca actgactcgg
     5581 agagaacgaa atccttccta cagaagagtg gacgataccg tacttatcca cacggaagga
     5641 aatttttcct ttcttcactt cagacactgc tgctccaact tcattggtta ctgtacctgt
     5701 tttagggtta ggcatcaagc ctctaggacc aagaatcctg gctattttac ccattttagc
     5761 cataactccc ggcattgcaa ccaccacgtc aacatcagtc cagccagctg caaccttgtc
     5821 aattaattca tcaagaccga catattcggc acctgcagct ttagcctcat cttctttttc
     5881 cggggtacaa aaaactgcaa ctgttttggt ttttccggtt ccgtgcggca gggaaactgt
     5941 accgcgaata gcctggtcgg cctttcttgg atccacgccc aatttgatgt gaacatcaac
     6001 cgatgcatca aacttggcat agttggcatc ttttacgaca gccattgctt cttcgatggg
     6061 atagaactta ttggcatcta tcttatcttg agaagctttc tgcttttttg ttaatttaga
     6121 catctcaaaa tgttttaagg tatatcgttg gaggaatcca actcccttca gttaataaat
     6181 atttacatct gacccatccg gatcgttaat tttcaaatca attgtcccag ggtgctgtac
     6241 cttcaacggt aatgcccagt gacctagctg taccggcaac catcttcata gcgctttcga
     6301 ccgtaaatgc attcaaatcc ggcatcttgt cctcagcaat ggctctaacc tgatcccaag
     6361 tcacttttcc gaccttattt ctgtttgatt ctgccgaacc tttctttgcg cctgacaatt
     6421 ccatcagctg cacagaggcc ggagcggttt taactacaaa ttcaaaggac ttatctttaa
     6481 acaccgtaat aagaaccggg cagactttgc ccattttatc ctgggtttct gcattgaatc
     6541 ttttacagaa ttccatgata ttcacacctt tggcacccaa tgcaggacca acgggtggag
     6601 cagggtttgc ctggcctcct tttacctgca atttgatttt atattcaacc tcttttgcca
     6661 tgataaataa ttatttgtat tgtctttaat aattcagttc cggatgattc tatttcttct
     6721 cgacctggac aaagttcaat tcaacttcag tacccctgcc aaatattttt actataacct
     6781 ttaacttctt cttttcgtca ttcacctctt tgatatctcc gacaaattcg ttgaatgggc
     6841 catcgatgat cttgacggtt tcacccacga taaacggttc gagcattgct tcatccatat
     6901 cctgggactc atcgatctta ccgagcagtc gattagcttc ggcgtcctgc attggaattg
     6961 gtttctggcc gcccagaaaa tgtatgacat taggtatatt attgattgtc tggataattt
     7021 ctccggtcat tttcttaaat accgcttcaa tcaaaatata gccaccaagg atatttcttt
     7081 ctctgatgac tttcttccct ttttcaatct tatatacctt ttcggtagga acaactacct
     7141 gggtaataat atccttccac cctgagcgca aaagttccag gtcgattgct tccttagctt
     7201 ttttttcctt accgctgata actctgagtg aataccattt tcgatcactc ggaaccgtta
     7261 tttcaggagt ttcagacatt tattatttta ttttatagat aaaatcaaac agtgttttat
     7321 tcaaaccgtc cattccaagt acaagcaggg aaaggatcaa taatccaacg atggtagcta
     7381 cagtactttg aaccaacata actccggtcg gccattctac tttatgcacc aattcatcat
     7441 aagattcttt caagtaagaa gttattttat ccatattaat aacatttgca cgggaagcag
     7501 ggatcgaacc cgcaacctgc ggttttggag accgccactc taccagttga gctattcccg
     7561 taattaagtg aaataagcac cccattaaac atgaggtgct taatatcatc aaattctttt
     7621 cagaaaatta ttattcaatg atctcagtca cctgtccggc accaactgta cggccacctt
     7681 cgcgaatcgc aaaacgaaga cctttctcca ttgcgatagt gcttagcaag gtaacatcca
     7741 gtgaaacatt atcaccaggc ataaccatct tcacatcagc cggaagttga caatctcctg
     7801 taacgtcagt tgttctgaaa tagaactgag gacgatatcc gttgaagaaa ggagtatgac
     7861 gtccaccttc ttctttgctt agtacgtata cctcacactt gaactttttg tgtggcttaa
     7921 ctgaaccagg agcgcagata acctgacctc tcttgatctg agtcttgtcg atacctctca
     7981 gaagcagacc tgcattatca ccagcttcac cgcgatcgag tagttttcta aacatctcga
     8041 caccagtcac tgtagatgta agtggcttct cgccttcttt catcatacca atgatttcaa
     8101 cagggttgtt tacgttgat
//
LOCUS       DAOQQN010000111         8112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3473_length_8112_cov_5.542386, whole genome shotgun sequence.
ACCESSION   DAOQQN010000111 DAOQQN010000000
VERSION     DAOQQN010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8112
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3473_length_8112_cov_5.542386"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PLG24_05615"
     CDS             complement(<1..282)
                     /locus_tag="PLG24_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HMZ24401.1"
                     /translation="MSWFKRLKEGITTTTKNKKEAPDGLWYKCPICKEASTMKEFREN
                     FYKCPKCDYHSRIGSREYFEIILNPGYTVLFNNIESEDFLHFVDLKPYKD"
     gene            479..1204
                     /gene="dapB"
                     /locus_tag="PLG24_05620"
     CDS             479..1204
                     /gene="dapB"
                     /locus_tag="PLG24_05620"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HMZ24402.1"
                     /translation="MKAALIGYGKMGHVIESVLLEKGHSVSFRISEENKGEIHDIRPD
                     NTDVCFEFTGPNSAFDNLTILIQQGLPVVCGTTGWYARMPEIYDLVKKQGTSFVAASN
                     FSIGVNLFFEAARQLSALMKHRGYSVEINETHHIHKKDAPSGTAITLSEQVRAVLEEF
                     EGYQLNSENEDNLIPIFSHRLEDVPGTHELRFGNEIDAISLKHVAFNRNGFALGAVMT
                     GEYIIGRKGIFTIGEILKHTMYS"
     gene            1803..2179
                     /gene="rnpB"
                     /locus_tag="PLG24_05625"
     ncRNA           1803..2179
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLG24_05625"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2265..2696)
                     /locus_tag="PLG24_05630"
     CDS             complement(2265..2696)
                     /locus_tag="PLG24_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B"
                     /protein_id="HMZ24403.1"
                     /translation="MLTGFKHLHSYFAYLLLAVLIIGILFNFLAFMQGKSYSNTNRKF
                     ALYGLMATHIQVLFGLILYFVSPLGISALSGEAMKDSTSRLYALEHPLMMILAVVLVT
                     IGYSKSKKATADARKFRIQLAFYAVALVFILSRIPWAAWPH"
     gene            complement(2801..3325)
                     /locus_tag="PLG24_05635"
     CDS             complement(2801..3325)
                     /locus_tag="PLG24_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24404.1"
                     /translation="MVKAKLISDTASYKKTKRLLIWTYTGLLTLYLLYNQVLIGYLGF
                     SPLPLLQYLNLAIVTTIIAAILMLLLGKKLASLIGQGKIEMDEKHLSILKAGTIVQTI
                     DLRPEDKVVLNIVNPFDSLATCKEYKGDSPVSFIEVYKNGGSVANRFDFVIESHYMMT
                     RLEKLKANWASMQK"
     gene            complement(3501..4403)
                     /locus_tag="PLG24_05640"
     CDS             complement(3501..4403)
                     /locus_tag="PLG24_05640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMZ24405.1"
                     /translation="MSSESVLNSLLILTAILVAIEAIVSSIQGRQFYTVKDTLYNFYL
                     MALNTGLAFLMLGVSIKVLDFFYQYSLPIHLNPFLYWLALLIVLDFAYYVLHVVDHQS
                     RFFWAIHVTHHSSELFNLTTGFRSSVLQPLYRFIYFIPLSLLGFKTIDILFMHSICQT
                     WGVLVHTKYIKKLPAWIEYIFVTPSHHRVHHASNVLYLDRNMGMFLIIWDRIFGTFQA
                     EEEWEKIRYGLTHEQEKVNPANIITHEWSAIWKDMKKSSNWLDKIKYIFYPPGWSHDG
                     SMKTSNQLRAEMYPQNVRDTDPKG"
     gene            complement(4534..6075)
                     /locus_tag="PLG24_05645"
     CDS             complement(4534..6075)
                     /locus_tag="PLG24_05645"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HMZ24406.1"
                     /translation="MVGQIVEKAFRVANLLKRPLIDPSSSQKEVLTQLMIKAQNTSFG
                     LHYKFSSLLYESDIVEKFRSTVPIFDYDKIHNEWWHRLLECEPDVCWPGRVRNFALSS
                     GTSGSASKFIPVSRDMQRSMRKAGLRLFMNMANVGIPAHMFNKAMLMIGGSASLTEKY
                     DYRYGDLSGINGASPPFWIKGYYKPGTEIAKIKDWNERTRIIVENAHKWDIGYLTGIP
                     SWVQMTLAAIIEHYKLKTIHDLWPNLSVFVTGGVNYQPYEASFNEMFAKEVIIIDSYL
                     ASEGFIAFQSRTETNAMRLLLDNGIFMEFVPFNEENFDEDGNIYPHAKTKLISEVDTE
                     TDYALLLTTNSGAWRYLLGDLIRFSNLQHQEIHIVGRTKHFLSVCGEHLSVDNMTSAV
                     LQVQKELGLHISEFTASYVEHEDFFRHKWYLACDSQASINDIKKVLDSKLCEINDDYD
                     TERRAGVLKEPAVEIIPTQWFYDYMERQGKLTGQTKFPRVIKHQAWEQWERYVLEKQG
                     ILSPR"
     gene            complement(6273..7097)
                     /locus_tag="PLG24_05650"
     CDS             complement(6273..7097)
                     /locus_tag="PLG24_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HMZ24407.1"
                     /translation="MARFLILAILLQSVYALHAQRGFEVGGFAGTSWYFGDINNDLKL
                     NSPGATIGAATRFNFDERICMKAGINYIWLRGYDYKSSNIYQKARNISFNNHMAELSG
                     QLEMNFLTYRHGSRDNWYTPYMFVGGSVFAHSPQAYYNGSWVKLRPLGTEGQKKGSEY
                     AITSASWLFGVGFKMDLTDTWSFNVELSSRQAFTDYLDDTHGYYADNFQLEYERGPDA
                     AYLADPSILIPGYNDAKIGIEGFQRGDKNNYDGYTTFTVGIMYYFARVPCPEILKH"
BASE COUNT         2288 a         1520 c         1788 g         2516 t
ORIGIN      
        1 gtccttgtag ggcttaaggt ccacgaaatg aagaaaatct tctgattcga tattgttaaa
       61 taaaactgta tagccgggat tgaggatgat ttcaaaatat tccctggatc ctattctgga
      121 gtggtagtcg catttgggac acttatagaa attctccctg aattccttca tggtgctggc
      181 ttccttacat atcggacatt tataccaaag tccatccggt gcttccttct tgtttttggt
      241 tgtggtggtg attccttcct ttaatctttt aaaccagctc atagttctaa ctccctgatg
      301 ataaatcaat tagttgttga atgttgtgtg caaatgaatg gattggtacc attaaccttt
      361 gctgcaatcc agcaaaagta caataatttt ttctaaatgg agaggttgga attttgaggt
      421 ttagtgaaaa gaagaagtta gtgatcgcag acttcaatat ttgttttacc tttataacat
      481 gaaagcagca ttgattggtt atggaaagat ggggcacgtc atagagtctg tcctgttgga
      541 gaaggggcat tcagtctctt tccggataag tgaagagaat aaaggcgaga tccatgatat
      601 ccggccagac aatacggatg tttgtttcga attcaccggt cccaactctg cctttgataa
      661 tctgaccatc ctgatccaac aaggtctacc tgtggtttgt gggacgacag gctggtatgc
      721 caggatgcct gagatctacg atttggttaa gaagcaggga acttcttttg tggcggcatc
      781 gaatttcagc attggtgtca acttgttttt tgaagctgcc cgacaattgt ctgcattgat
      841 gaagcaccgc gggtattcag tcgagatcaa cgaaacccac cacatccaca aaaaggatgc
      901 tccgagtggt actgctatta cattgagtga acaggtacgg gcggtgttag aggaatttga
      961 aggttatcaa cttaactctg agaatgaaga caatctgata ccgatctttt ctcatcgact
     1021 tgaggatgtt cccgggacac atgaattgag gttcggcaat gaaatcgatg caataagcct
     1081 gaagcatgta gcattcaacc gaaatggatt tgccctgggt gcagtaatga ccggtgagta
     1141 tatcattggt cggaaaggta tatttaccat tggggagata ttgaaacata ccatgtactc
     1201 ttgacagggg atctgattga tgagttgatt attcctgttg aaactgtttc aatgcacgcg
     1261 tgaaataaat atctgttggg ggtaatcatg tacttcaaat tgactctcat ctttgattgc
     1321 ccctacgggc tttctgaatg ttaaaaagta tgaaacggcc ttttaaaacc ggattttttt
     1381 gcacggtctt ggatttttca gtggtagaaa aagtaatgta tatttcatga tcaatgatta
     1441 tgtttcggga ggatgacatt gaaaaaaggt agataataac gaatgtaaac tcggcatccc
     1501 ggaaccccgg attgtttcac tcacgggaca gagcagagct atagtagcat agctatccgg
     1561 acaagtatac caagcatata tggaatattc tataaatcat cttggtatag caaccttaat
     1621 ctgtcaacaa atgtgtgatg ttctttttga tgagatacat aggtatctga tttttattat
     1681 tgctcaatgc agcgcttcaa taagagaagg tagggacaat gtttgttgtt ttacatgctg
     1741 aaagtagatc taccccggaa ttttctacct ttgatatgcg ttattaacaa attgaagtca
     1801 agataacatt gtacttcgtc gcgctctgca atggcagggt gaggaaagtc cgggcaacat
     1861 agagcagcgt accacctaac gggtgggtct tgcagccgtg caggaacaga gagtgtcgca
     1921 gaaactatac cgccttgcaa aagcaaggta agggtgaaaa tgtgcggtaa gagcgcacac
     1981 ggtgtgccgg taacggtgcg ccgggataaa ccttacgcgt tgaaatgcca agtatatccg
     2041 atgtctacgg catatgccat agagtggtgt ggctcgcacc ttgtcggagg ggtgggcagt
     2101 aaagagccgt ccggtgacgg ccggccaaga taaatgacga agctacgtac agaatccggc
     2161 ttattgcaat gttatcattt tgattttgaa aaagaaaaag cgcttcttga agcgcttttt
     2221 cttttatatg tcggaattga aattatcctt tattagtgca tttgtcaatg tggccatgct
     2281 gcccaaggga ttctggaaag tatgaataca agtgccaccg cataaaacgc aagctgaatt
     2341 ctgaatttcc gtgcatcagc tgttgctttt ttagacttgc tgtatccaat agtaactaaa
     2401 acaaccgcaa ggatcatcat gagtggatgt tccaatgcat agagtcggga tgtactgtct
     2461 ttcatggctt cacctgatag cgcagagatg ccgagtggcg agacaaaata taatatcaaa
     2521 ccgaatagaa cctgtatatg cgtcgccatc aatccatata acgcaaactt tcggtttgta
     2581 ttggaatatg attttccttg cataaacgcc aggaaattga aaaggatgcc tatgataagc
     2641 actgcaagta gcagataggc aaaataggaa tgtaaatgtt taaatcctgt caacatattt
     2701 atttttttaa agcatgaaga tacaggatta tttagacaaa ttacattttg tctttgataa
     2761 atgattaatt aaattggaaa tgacgaaaga tggctgcaga ctatttctgc atagatgccc
     2821 aatttgcttt taatttttcc aaccgggtca tcatataatg agattctatg acaaagtcga
     2881 atctgttggc tacgctacct ccatttttat aaacctctat aaaggatacc gggctgtccc
     2941 ctttatattc cttacaagta gctaatgaat caaaagggtt gacgatgttc aggacgactt
     3001 tgtcctccgg tctcagatca atggtttgta cgattgttcc agctttcaga atggaaagat
     3061 gtttttcatc catttcaatt ttaccctgac cgatcagact ggctaacttt ttcccaagca
     3121 ataacatcag gatggctgca atgatagtcg taactatagc aaggttaaga tactgtagta
     3181 aaggcaacgg tgaaaagccc aaatatccta taagtacctg attgtacaac agatacaagg
     3241 taagcagacc ggtgtatgtc catatcagga gtcttttggt ttttttataa gaggcggtgt
     3301 ccgaaatcag ttttgccttt accatgatta caaaattgag aaccaaattt acattgaatc
     3361 gtttaaattt acaaattttt caagagataa atgcgttttt ctcaatatcc cctgcattga
     3421 gcatgaggga cttctatgat aaatccaaat catgaagaca atatttatgt tcggggaagt
     3481 ttaatttaac ttcagggcaa tcagcctttc ggatctgtat ctctgacatt ttgcggatac
     3541 atctctgcac gcagttgatt gctggtcttc atacttccgt catggctcca accgggaggg
     3601 tagaagatat atttgatttt gtctaaccaa ttactggact ttttcatgtc tttccaaatg
     3661 gcggaccatt cgtgggtgat tatgttagca gggttgacct tttcctgctc atgtgtcagg
     3721 ccgtacctta ttttttccca ttcttcttca gcctggaagg tgccgaatat tctgtcccaa
     3781 ataatcagga acatgcccat attacggtcg aggtacagga cattggaagc gtgatgtact
     3841 ctatggtgag acggagtaac aaagatgtat tcgatccatg caggaagttt cttaatatat
     3901 tttgtatgaa ccaaaacacc ccaagtctgg catatagagt gcataaacaa gatgtcgatg
     3961 gtcttaaagc cgagtaatga caggggtatg aagtaaatga agcgatagag tggttgtaag
     4021 acggacgatc tgaagcctgt cgtgagatta aataattcag agctgtgatg agtaacatgg
     4081 attgcccaga aaaatctgct ctgatgatcc accacatgca ggacataata ggcaaagtcg
     4141 agtacaatca gtagagccag ccagtataaa aaaggattga gatggattgg aaggctgtat
     4201 tgatagaaaa agtcaagtac tttaatagat actcccagca tcagaaaagc cagccctgta
     4261 ttcagtgcca tcaagtaaaa attatacaag gtatctttga ccgtatagaa ttgtcttccc
     4321 tggattgaag agacaattgc ttctatggct accaatatgg cagttagaat aaggaggcta
     4381 ttcagtactg attcggaaga catatttttg aaaatttttt aaaagagtcc aattaaggtt
     4441 agattgaagt ttgcctataa ttcaggtgaa cttgtgtttg accatttttt tattgcgcaa
     4501 ttctgattct tcacacaggt gagtttctgc agtttacctt ggcgaaagta tgccttgttt
     4561 ttcaagcaca taccgctccc attgttccca agcctgatgt ttgattactc gggggaattt
     4621 ggtctgtccg gtcagtttgc cctgacgttc catataatca taaaaccatt gtgttggaat
     4681 tatttccaca gcaggttctt tcaggacgcc ggcccttcgt tcggtatcat agtcatcgtt
     4741 gatctcacac agcttggagt cgagcacttt tttgatgtca ttgattgaag cttggctgtc
     4801 acatgccaga taccatttat ggcggaaaaa atcttcgtgt tctacatatg aagctgtgaa
     4861 ctcactgata tgaagtccta attctttctg cacctgcaac acagctgaag tcatgttatc
     4921 tacggataga tgttcgccgc agacgctgag gaagtgtttg gttcggccta caatatggat
     4981 ttcctgatgc tgaagattgg aaaaacgtat cagatctccc agtaagtatc gccaggcacc
     5041 gctattggta gtcaagagca atgcataatc agtttcagta tcaacttcac ttataagctt
     5101 tgttttggcg tgtgggtaaa tgttgccgtc ttcgtcaaag ttttcttcat taaaagggac
     5161 aaattccatg aagatgccgt tatccagtaa aaggcgcatg gcattggtct ctgttcggga
     5221 ttggaaggca ataaatcctt cagaagccag gtagctgtct atgataatta cctccttagc
     5281 gaacatttca ttgaagcttg cttcatatgg ttggtagttg acacctccgg tgacaaacac
     5341 actgaggttt ggccacagat cgtgaatagt ttttaatttg tagtgttcaa tgattgcggc
     5401 gagagtcatc tggacccagg atggtatgcc tgtcaggtag ccaatgtccc atttgtgggc
     5461 gttctcgact ataatgcggg ttctttcatt ccagtctttg attttggcta tctcagtacc
     5521 cggtttgtaa taacctttga tccaaaaagg aggggaggcg ccattgatgc cactgagatc
     5581 tccgtatctg taatcgtatt tctccgtgag tgaagcggat ccgccgatca tcagcatcgc
     5641 tttgttaaac atatgcgcag gaatgcctac gttggccata ttcataaaca gtcgcaagcc
     5701 ggctttgcgc atggatcgtt gcatgtcgcg tgagacaggg ataaatttgg aggctgagcc
     5761 ggacgtgcct gagcttagag caaaattcct cactctccct ggccagcaca catcaggttc
     5821 gcattccagc agacggtgcc accattcatt gtgtattttg tcatagtcaa atatcggtac
     5881 cgtagaacgg aatttttcta cgatgtcaga ttcatataag agggatgaaa atttgtaatg
     5941 aaggccaaaa gaagtatttt gagcctttat catcaattga gtgagaactt ccttctgaga
     6001 tgaagaaggg tctattaatg gcctctttaa cagattggcg acgcgaaatg ctttttcaac
     6061 aatttgtcct accacagagt aaagaaattt cgataattaa tattttggtt aatgttcaaa
     6121 aagaaaagaa ttaattggcc ttaaaattta taaataatac aaataaaagc caattttatt
     6181 gaaaaatttt ctttttctgg aattttttac ccaaattcaa ctacgaaaat acgcaaaaga
     6241 actgatttta gttatttttt tgataaattt tatcagtgtt ttaatatttc cggacacggg
     6301 acgcgtgcaa agtaatacat aattcccacc gtaaaggttg tatagccgtc ataattattt
     6361 ttatcgcctc gttgaaatcc ttctatccct attttagcat cattataacc cgggatcagg
     6421 attgacggat cagccagata agctgcatcc ggtccccgtt cgtattctag ttggaaattg
     6481 tctgcataat aaccatgggt gtcatccagg taatcggtaa atgcctgccg ggagctcaat
     6541 tctacattga aactccatgt gtcggtcagg tccattttga acccgactcc aaacaaccag
     6601 gatgctgaag taatggcata ttcgctgccc tttttctggc cttctgtacc taggggcctt
     6661 agtttgaccc aactcccgtt gtaataagcc tgtggagaat gtgcgaagac tgaaccaccg
     6721 acaaacatat aaggagtata ccagttgtct ctactgccat gtctgtatgt aaggaagttc
     6781 atctccagtt ggcctgacag ttcagccata tgattgttga agcttatgtt ccgggccttc
     6841 tggtagatat tggagctttt ataatcatag cctctcagcc agatatagtt gatcccggcc
     6901 ttcatacata ttctttcgtc gaaattgaag cgtgtggctg caccgatggt tgctcccggg
     6961 ctgttgagtt tcaggtcatt gttaatatcc ccgaaatacc agctggtacc ggcaaagccg
     7021 cctacctcga agcccctttg agcatgcaag gcatataccg actgtaataa tattgctaaa
     7081 ataagaaatc ttgccaaaag atagtttttg aaataaacgg caaaaatacg taaattctta
     7141 ttggtggttt aatattatag catggattga gaagttttct tattatttga ctctgtgtat
     7201 tggttatttg ctgttaccgg aaggcaagaa gacatgatgc ttgtggagga aagaccggtg
     7261 taacggagaa agatcaagaa gggttgtttt ctgaaaattt tttatcattt tcaaccacat
     7321 gaaagcataa ggctcatatc atttgggtta tttttttatt tgtattttag ttttgtggct
     7381 tactgtggtt gatttattca ggaattaggt agaagattaa tagcggtata ttcaagggac
     7441 aagccttttt cgaattgtgc agtaaataac tttcccattt ttgagcatat aaatatttgc
     7501 ttttcaagtg agatgcaggt gtcacacaga tcgtggagta ttattttcct acctacttcg
     7561 atttttggga gggagaggat gatgtcgcct ccgaagtagg tagatgatta atagtcacaa
     7621 tgcttgcagg tattaccttt cggggcagcc ataccaaaaa ttcgatgatt cactcgggaa
     7681 tttaattttt catttgtctc caaatccttg tgtatagcgg tttttgaatt attactttcc
     7741 tacctacttc gatttttggg aggatggtgg tggtgtcgcc tctgaagtag gtagatgatt
     7801 aatagccaca atacttgcag gtattacctt tcggggcagc cataccaaaa attcgatgat
     7861 tcacttggga atttaatttt tcatttgtct ccaaatcctt gtgtatagcg gtttttgaat
     7921 tattactttc ctacctactt cgatttttgg gaggatggtg gtggtgtcgc ctctgaagta
     7981 ggtagatgat taatagccac aatacttgca ggtattacct ttcggggcag ccataccaaa
     8041 aattcgatga ttcacttggg aatttaattt ttcatttgtc tccaaatcct tgtgtatagc
     8101 ggtttttgaa tt
//
LOCUS       DAOQQN010000112         8024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3540_length_8024_cov_5.394654, whole genome shotgun sequence.
ACCESSION   DAOQQN010000112 DAOQQN010000000
VERSION     DAOQQN010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8024
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3540_length_8024_cov_5.394654"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PLG24_05655"
     CDS             complement(<1..492)
                     /locus_tag="PLG24_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ24408.1"
                     /translation="MKRIRLVFVLLIFPLSIIFSQNLEYKNGIGISGRIGNGDLLNDR
                     DFDMANSSLLIDLNYSRNLTSWLDVKIPLGVAMTMNNTVEDKLLGFHGDILFQLGLFD
                     RTRIAAPYLYLGPSFNLSKDFVENSYSVFDIAARAGLGVDFRITDNLLVGLNAGYTNT
                     FEEN"
     gene            complement(585..1517)
                     /gene="nusB"
                     /locus_tag="PLG24_05660"
     CDS             complement(585..1517)
                     /gene="nusB"
                     /locus_tag="PLG24_05660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HMZ24409.1"
                     /translation="MLSRRNIRVKVLQSLYSYFSTEGMKKDEVMRNYDESIENAYILL
                     HFQLYLLCKISEHSKEDLIKRQSKLLPTEYDKTFTAKFYENPLMQKIVNNDEIKRLSS
                     KYNFDEKIPEDFTRKLYKTFSLTPEYQNYVNKSNTIEDDQIILLSLFKDICKQEFFND
                     TIDDFFYNWQDDQSIIIGTIKKIIKDFKTSAEIAEEYGPSEEAIRDFGRELLKYILNH
                     NDDLEKHLTPIIENWDIDRVAAIDMILIKMSLSEMLIFPSIPTKVSINEYVELSKAYS
                     TDKSKDFVNGILDKALHTLSDKKLISKSGRGLAE"
     gene            complement(1546..2997)
                     /locus_tag="PLG24_05665"
     CDS             complement(1546..2997)
                     /locus_tag="PLG24_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HMZ24410.1"
                     /translation="MTIVKLMTWIGIAALLITVITVYLKKTKNPLFSFLQYFTGILFL
                     VSGFVKAVDPLGTAFKTEQYFDAFYYTFEGTKMSFIAPLFHFLNNYVNAFSIGTIVLE
                     MMLGVMLILGIKPRLTSWAFFLLMIFFTCLTGFTFLTGYVPPDVNFFSFSKWVEFSES
                     NMRVSDCGCFGDFIKIVPKTSFLKDIFLMIPAILFLLYNKKFHKLLSSGTRVGISTVS
                     FIAFLFYCLNYTYWNEPDIDFRPFKEGTNIREQKIIEQEAEASRPVFQVITPKNGGTS
                     VTLSSEEYAKRWKEFPKEDFDIEQKTGEASMKITKISDFRLTDMDEADVTDNILSDSA
                     YSLMIVNYKVHFDFEMKQVTSKDTTFVEGPGDSIHIEGIKSVTQNVEMPVFRNDWKTM
                     YESKILPLIDKAESKHMSAYVVFGGLSIDQMKEASRFIGLEVPVYQADETILKTVIRS
                     NPGIILWKNGKIIKKWHINKAPEWAEIEGYMKD"
     gene            3833..6460
                     /locus_tag="PLG24_05670"
     CDS             3833..6460
                     /locus_tag="PLG24_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ24411.1"
                     /translation="MSQVEMPQPKKKYKFKELKCYASTEWLIDNQKKYRQVFDRYEVS
                     YIYAELSFINKMYDEEDWSVRIDLKCYQVIDNNQRNEICSLHFDRTIYKYDSIVYLRE
                     GWGAKQEGHFWKQGSYYWEVYINGEKEGTKHFYVIDADRPMSDEDNPFFSLLSVKLYE
                     GPYGDVPEDERTYYTEFSGSESQLIYMEMCIDNFLPKTWQCEVFIRIYTSSRELKGQI
                     VRLVNVKPNDSEIKITAGWGTNVKGNWKRGNYTYEVVFMDRLMAIVPFTVGEFFIEGH
                     PAVFLQDPLIPLEFKEIEHDEENFESIMSKIDRYIGLEDIKRAIRQHAAYINFLNIRK
                     QKGLESDAKLNIHSVFIGNPGTGKTTIAGFIGRLYKKMGLLSKGHVHEVDRVDLVGEY
                     IGQTAPKVKQAIDTARGGVLFIDEAYSLARTNDDSKDFGREVIEILVKEMSSPTCDFA
                     VIVAGYPKEMKHFLNSNPGLNSRFKMYYEFPDYLPQELSEIADYVAGDLKVRFTPKSK
                     AMLDEIILEAFRKKDKTFGNARFVYDLVEKGKINLGIRTMSRANPERLSDEQLSTITE
                     QDILKLKQVIRPNLPEIPIDEILLAEARNELESLIGMDNIKKDILEVVEIVRYKRRHG
                     ENLLHKFFFHTILIGNPGTGKTTVSRILAKLFKALGILERGHLVETDRQGLIAGFVGQ
                     TAIKTTEKIDEAIGGVLFIDEAYAIGGSGFNDYGNEAIQTLLKRMEDDRGKFYVFAAG
                     YPDNMDAFIKSNPGLASRFDKTLKFYDYSPEELIEIARKMLADQHYRLSPKALEYLAQ
                     YINFLWQYRDKYFGNARTIRLITDELIKKHNLRLVRENEGHKERQRTNIITMDDVSFL
                     EMKTEDLNFVRKKIGFGSR"
     gene            complement(6620..7417)
                     /gene="proC"
                     /locus_tag="PLG24_05675"
     CDS             complement(6620..7417)
                     /gene="proC"
                     /locus_tag="PLG24_05675"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HMZ24412.1"
                     /translation="MKKTIAIIGCGNLGSAIAKGILEKSSDKYNVIATRNKVELLTPL
                     QALGVEVSNNNIDAVAKSEIILLAVKPYLVATILNEIKNVLNPAKVLVSLATGITIAD
                     MKLILGDTIPVLRAMPNTATGVGESLTCLCSADSNSEKRIGEVKNLFDLIGESIVIRE
                     DLMDGATVLGACGIAYVMRFIRAMVQGGIEIGFDAATASKIANQTVKGAAQLLILGQT
                     HPEQEIDKVTTPRGCTIVGLNEMEHQGFSSSLIKGIITSYKKIENTK"
     gene            7629..>8024
                     /locus_tag="PLG24_05680"
     CDS             7629..>8024
                     /locus_tag="PLG24_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HMZ24413.1"
                     /translation="MIPWEEILLRLVLASLFGAAIGLERERKTLAAGLRTHMMVCVGA
                     CLIMLVSSLGFADILGTPNVMLDPSRIASQVVSGIGFIGAGTILFMKQGTVHGLTTAS
                     GLWTVAAIGLATGGGMYFAAGVTTAIALII"
BASE COUNT         2504 a         1450 c         1577 g         2493 t
ORIGIN      
        1 attttcttca aaagtattgg tgtatccggc attcagaccg actaataaat tgtccgttat
       61 cctaaaatcc acaccaagac cggctctagc ggcgatatcg aaaacactgt aagagttctc
      121 gacgaaatcc ttcgaaaggt tgaaggatgg gcccagatac aaatagggtg ctgcaattct
      181 ggtgcggtca aataagccca gttgaaacag aatgtcgcca tgaaatccca ataatttgtc
      241 ctcaacggtg ttattcatcg tcattgctac tccaagcgga atctttacgt caagccacga
      301 agtaagattt cgagaataat tgagatcgat gagcaaggaa ctgttggcca tgtcaaagtc
      361 ccggtcattg agtaagtcgc cattgcctat ccggccggaa attcctatgc cgtttttgta
      421 ttcaaggttt tggctaaaaa tgattgaaag ggggaagatt aataagacaa ataccaatcg
      481 aatacgtttc atgtcaatga ttttaagaat tattccaata ataatataat accaattaat
      541 taatcattca gcaaatagat taagcccaac cgatctggga caatctattc tgccaggcct
      601 ctacctgatt tgctgattaa ttttttatct gaaagagtat gcaaagcctt atctaatata
      661 ccattaacga aatccttgct tttatccgta ctgtaggcct tggataattc aacatattca
      721 ttgatggata ccttagtggg gatagatgga aatatcaaca tttcgctcaa agacatttta
      781 atcaagatca tatcaatggc cgcaactctg tcaatatccc agttttcaat gataggagtg
      841 agatgtttct ccaaatcatc gttatggtta agtatatatt ttaataattc tcttccaaaa
      901 tcacgaattg cctcttctga aggaccatat tcttcggcta tctcagcact tgttttaaaa
      961 tccttaatga ttttcttaat tgtgccaatg ataatcgatt gatcatcttg ccaattatag
     1021 aagaaatcat cgattgtatc attgaagaat tcctgcttgc aaatatcctt gaataaagac
     1081 agcaaaatta tttgatcatc ctcaatggta ttcgacttgt taacataatt ttggtattca
     1141 ggggtaagac taaatgtctt gtataacttt cttgtaaaat cctcaggtat cttttcatcg
     1201 aagttatact ttgatgatag ccgcttgatt tcatcgttgt ttacaatctt ctgcatcaga
     1261 ggattttcat aaaattttgc cgtaaaagtc ttatcgtatt ctgttggtaa taatttggac
     1321 tggcgcttaa taagatcctc ctttgaatgc tctgaaatct tgcataacag atataactga
     1381 aagtgcaaca agatataagc attctcaatg gattcatcat aattacgcat gacctcatcc
     1441 ttcttcattc cctctgtgga aaagtaggaa tacagcgact gtaagacttt tactcttata
     1501 tttcttctac taagcattat atttttatcc tgttatgact tctgtttaat ctttcatgta
     1561 tccttcaatt tcagcccatt ccggtgcttt attgatatgc cattttttaa tgatttttcc
     1621 atttttccag agtataatcc ccgggttgga cctgatcaca gtttttaata tggtttcatc
     1681 tgcttgataa acaggcactt ccaaaccaat aaaacgagag gcttccttca tctgatctat
     1741 cgataaccct ccaaaaacga cataggcact catatgtttt gattctgctt tatcaatcaa
     1801 tggtaaaatt ttactttcat acattgtttt ccaatcattg cggaatacag gcatctcaac
     1861 attttgggtt acgctcttaa tcccttcaat atggatagaa tcacccggcc cttcaacgaa
     1921 tgttgtgtct ttgctagtca cctgcttcat ttcaaagtca aaatgtacct tgtaattgac
     1981 tatcatcagg ctataggcag aatcagaaag tatattatca gttacatcgg cttcatccat
     2041 atctgttaac ctgaaatcac tgattttcgt aattttcatt gaggcctccc ctgttttttg
     2101 ttcaatatcg aaatcttctt tgggaaactc cttccaacgt ttggcatatt cttctgagct
     2161 cagtgtgaca gaagttccgc catttttcgg tgttattacc tgaaaaaccg gtctgcttgc
     2221 ttcagcttct tgttctatta ttttttgttc tctaatattt gttccttcct taaatggtct
     2281 gaaatcaata tcaggttcat tccagtatgt ataattcaga caatagaata aaaatgcgat
     2341 gaagctaacg gttgatatgc cgactctggt acctgatgac aataatttgt gaaatttttt
     2401 attatataac agaaataaaa ttgcaggtat catcaggaag atatccttca gaaatgaggt
     2461 cttaggtacg atcttaataa aatcgccaaa acaaccacaa tcagaaaccc gcatgttact
     2521 ttccgaaaat tctacccatt tactgaaact aaaaaagttg acgtcaggcg gcacatatcc
     2581 ggtcaggaac gtaaatccgg tgaggcaggt aaagaatatc atcagcaaaa aaaaggccca
     2641 ggatgtcaac ctgggtttta tccccagaat caacataact cccagcatca tttccagtac
     2701 aatggtgccg atagagaatg cattgacata attatttaaa aaatgaaaca acggggcgat
     2761 aaagctcatt ttagtgcctt cgaaagtata ataaaaggca tcaaaatact gttcagtctt
     2821 gaaggctgta ccaagcgggt caactgcttt gacaaaaccg gatacaagaa aaagtattcc
     2881 tgtaaaatat tgtaaaaatg agaacagcgg gttctttgtc tttttcagat aaacggttat
     2941 gacagttatt agcaatgctg caatacctat ccaagtcatc aatttcacta tcgtcattca
     3001 aaaacaattt ttaaatcaga aaaagtaaat ataggaagcc aatatccggg tgagtcagag
     3061 ggtttgaagg aaattaaaac agaatcacgt tgtgattgta ctaaatcggg tgttacgaac
     3121 atcgtaaatt ttctttcatg tgtatataaa ataacaagag gcttttccac ttgaaatgtc
     3181 aattagctaa aaataagttt gttctttctt atgacaaagg tagttaaaat atttaattag
     3241 ttataatata tgtgatcatt tttctggtat cgtaaaatat ctacaaaaat ttatttgatg
     3301 caacttttga ttttattttt ttgaaataat ttatttgaca cttaaacaac cttgactact
     3361 atcttggatt tatgtttgga tggttgtgaa atatttgata ttattattac gggagtaagc
     3421 tgaaaatatt gaagttgtcc tccataatta aacctatgtc agaaaatatg cgagattgtc
     3481 aagttacgag tttggtaccg ttccgggttt gtttttggtc ttcaatgata aaataatgaa
     3541 cctgagatgt gattgcacaa gatacttaac atggctgtag ttgaagttgc gatggaattt
     3601 tacacggtcg tttttcctca aattttaaga atattttaaa ttatggtaat ttatccctgc
     3661 ctggttgtat ctattaaatt atatctcgat tcttggacaa tccatctttt aatcaggtaa
     3721 ggtaaaaatg gaaaatgcaa ataaaaatga attaatattg cgttattaat aattttaaaa
     3781 aatgcatata ttgcacaaca attaacccac aaccaacaaa ttaaactgaa agatgtccca
     3841 ggtcgaaatg ccccagccta aaaagaagta taaattcaag gaattgaaat gctatgcatc
     3901 tacggaatgg cttattgata accagaaaaa atatcgtcag gtatttgata gatatgaagt
     3961 cagttatatc tatgctgagc tgtcatttat caataagatg tatgatgagg aagattggtc
     4021 ggtaaggatt gatctgaagt gttatcaggt tattgataat aatcaaagaa atgaaatttg
     4081 ctcattgcat tttgacagaa cgatatacaa gtatgattca attgtttatc ttagagaagg
     4141 ttggggtgcg aagcaggaag gtcatttttg gaaacaagga agttattatt gggaagtata
     4201 tatcaatggg gagaaggaag gtactaagca tttttatgtg attgatgccg acaggcctat
     4261 gagtgatgaa gataatccat tcttctcact tttgtctgtt aagctatatg aaggtcctta
     4321 tggtgatgtt ccggaagatg agagaacgta ctacactgaa ttctcaggta gtgaatcaca
     4381 gttgatctac atggagatgt gtattgataa ctttctgccc aaaacctggc aatgcgaggt
     4441 gtttataagg atatatacca gtagccggga gttgaaaggg caaatagtgc ggttagtcaa
     4501 tgtcaagccg aatgatagtg aaataaaaat taccgccggt tgggggacca atgtaaaagg
     4561 aaactggaag cgcggaaact acacttacga ggttgtcttt atggacaggt tgatggctat
     4621 tgtaccattt actgtcggcg aatttttcat tgaaggacat ccggctgtct tcctgcaaga
     4681 tcctttaatc ccactggaat ttaaagaaat cgagcatgat gaggagaact ttgaatcaat
     4741 catgtcaaaa attgatcgtt atattggact ggaagatatt aaaagagcaa taaggcaaca
     4801 tgctgcttat atcaatttct tgaatattcg gaagcaaaaa ggtctcgagt cggatgccaa
     4861 gctaaacatt cattccgtct tcattggtaa tccggggaca ggaaaaacga ccatcgccgg
     4921 atttattggc cggttgtaca aaaaaatggg ccttttgtca aaaggtcatg tgcatgaagt
     4981 ggacagagta gatctggtgg gagaatacat cggtcagaca gcacctaagg tcaaacaggc
     5041 tattgatacg gcgagagggg gcgtcctctt tatagatgaa gcatattcac tcgcgagaac
     5101 caatgatgac tctaaagatt ttggcaggga ggtcatagaa atcctggtga aggaaatgtc
     5161 cagccctacc tgcgattttg ctgtgatagt agccggatat ccaaaggaaa tgaaacactt
     5221 cctcaattca aatcccggct tgaattccag attcaaaatg tattacgaat tccctgatta
     5281 tttgccgcag gagttaagtg agattgctga ttatgttgcc ggtgatttga aagttagatt
     5341 tacccccaag tcaaaggcta tgttggatga aataatcctg gaagcattca gaaaaaagga
     5401 taagacattt ggcaatgcgc gttttgttta tgacctggta gaaaaaggaa aaattaatct
     5461 tgggataagg actatgagcc gtgcgaatcc tgaaagactt agtgatgaac aactgtcgac
     5521 aattaccgaa caagatattc taaaacttaa acaggttatc cggccgaatc tgcctgaaat
     5581 accaattgat gagattcttt tagcagaggc aaggaacgaa cttgagtcgc tgattggtat
     5641 ggacaatatc aagaaggata tccttgaagt agtagaaatt gtaagataca aaagaagaca
     5701 cggtgaaaat cttcttcata aatttttctt ccacaccata ctcattggta atcccgggac
     5761 aggaaaaacg actgtttcca gaattcttgc caaactgttc aaggctcttg gaatattaga
     5821 aagaggccat ttagtggaga ctgaccgaca aggcctgatt gcggggtttg tcggtcagac
     5881 agctattaag accacagaaa agattgatga agcgattgga ggagtattgt ttatcgatga
     5941 ggcatatgca attggcgggt caggcttcaa tgattacgga aatgaagcca tccagacctt
     6001 actcaaaaga atggaagatg atcgtggaaa attttatgtt tttgcagcag gttatcctga
     6061 caacatggat gcattcatta aatcaaatcc cggcttggcc agtcggttcg acaaaacctt
     6121 gaaattttat gactactctc ctgaagaact cattgaaatt gcaagaaaga tgttggcaga
     6181 ccaacattac aggctgtctc ccaaggcatt ggaatatctt gctcaataca taaactttct
     6241 gtggcaatat cgtgacaaat actttggcaa tgcccgaaca atacgactta tcacggatga
     6301 gttgatcaaa aagcataatt tgagactggt cagggagaat gaaggacata aagaacgtca
     6361 acggactaac atcattacaa tggatgatgt ttcattcctt gaaatgaaaa cagaagattt
     6421 gaactttgtt cgtaaaaaaa ttggcttcgg cagcagatag cggccagatg gattccagca
     6481 tctccttcat tcaggtttga tttgatactt atttgtatag accggttttc ttgtcctgtt
     6541 atagattata agtgctgata tacttagaaa gtgaatttct gcgtaggtta atgaacacaa
     6601 ctaaacaccg gtttcaattt tatttggtat tctcaatctt tttgtaggat gtaattattc
     6661 ctttgatgag agaagaactg aagccctggt gttccatttc attcaatcct acaatggtac
     6721 aaccacgtgg tgtggtgact ttgtcgattt cctgttccgg atgagtttgg cccaatatta
     6781 gcaactgtgc agcacctttg acggtctgat tggctatctt actggctgtt gctgcatcaa
     6841 agccgatttc aataccgccc tgaaccattg ctctgataaa gcgcatgaca taggcaatac
     6901 cacatgctcc aagaacagtt gcgccatcca ttaaatcttc tctgataaca atagattcac
     6961 caattaaatc aaataagttc ttgacctccc ctatcctttt ctcagagtta ctatcggcag
     7021 agcataggca tgtgagggat tctcctacac cggtagccgt attgggcatt gctcttaaaa
     7081 cgggaatagt atcacccaag attagcttca tatcggcaat ggtaatacct gtagccagag
     7141 aaaccaatac tttagctgga ttcaaaacgt tctttatctc atttagaatg gttgcaacta
     7201 aatagggctt gaccgccagt agaataattt ctgatttggc cactgcatct atattattat
     7261 tggatacttc gacaccaagt gcttgcaagg gagtaagaag ctcaacctta tttctggttg
     7321 cgataacatt atacttgtca gagctcttct ccaatattcc tttggcaatg gcgcttccaa
     7381 gattgccgca accaatgatt gcaatagttt ttttcattta tacgaaattt tcaatggcaa
     7441 aggtagtaaa aaatcgatct tgtaaaattt aatccctttt ttaccagtca caaagtcaag
     7501 aatgatggtg ctaatccaat attgcaggat tcaaaaaata tgtctacctt cactgccgga
     7561 caaattagga aattaacatt tctaaagatt tattctgaaa tcaataggtg taataaatat
     7621 tgcaaattat gatcccctgg gaggaaattc tattgcgtct ggtcctggcc agcctctttg
     7681 gtgctgccat tggtctcgag agagaacgga aaacacttgc tgcaggattg cggacgcata
     7741 tgatggtatg tgtaggagca tgtctcatca tgctggtttc ttcattaggc ttcgctgata
     7801 ttttaggtac acctaatgta atgcttgacc cttcccgcat cgcttcacag gtagtaagtg
     7861 ggattggctt tattggtgcg ggaactatct tgtttatgaa acaaggtacg gttcacgggc
     7921 ttacgactgc ttcgggtctt tggactgtgg ctgccatcgg attagcaacg ggtggtggca
     7981 tgtatttcgc agccggagtt accacagcaa tagctttgat tatt
//
LOCUS       DAOQQN010000113         7998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3557_length_7998_cov_6.256830, whole genome shotgun sequence.
ACCESSION   DAOQQN010000113 DAOQQN010000000
VERSION     DAOQQN010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3557_length_7998_cov_6.256830"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2133
                     /locus_tag="PLG24_05685"
     CDS             <1..2133
                     /locus_tag="PLG24_05685"
                     /inference="COORDINATES: protein motif:HMM:NF027510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HMZ24414.1"
                     /translation="GGHTFNACNGSTQYNPFSSFEPGAGSTILSYGDNCGSDNVGERV
                     DYFHVANIIEVNRYVKEIENKCGTWGAEINHTPVGYLSMPEDKTIPILTPFELEGGAT
                     DDDKNLTFNWEQYDLGEGEPLGTNFETGPLFVSQAPGGVGHRLFPSLSTINSGIISNR
                     ERLPEVSREMNFKMTVRDNNPNVGAQAIVSYKFDVTDQAGPFKVTFPDAQTDTSFTLG
                     QYVEITWDVANTDQFPVSCKNVNILFSTTNGNTWSDTLVANTPNDGRESVMMPRTSGR
                     ARFKVKAADNIFLDLSGKPLKIVQPSEAGYSLDILPHDISLCKNSIYTLTVKSVSWKA
                     FNSPVKISVFDSGHPAFKVIPLKSEFLPGETMTLDVDVPDVIDAGTYQIRFMATSDQG
                     DTLFRTVTLTIADNDQNISGTISPVANDQYVTLHPVFAWDPVENTHGYQLQLATDPAF
                     SSLILNVSIDSEEYLSEIELNKATIYYWRVRSLGECGYGRWTAMSIFRTESELSNSAL
                     HQINNKILKVKTNEKKPALHEDLYYVSELNQNESLKYTLLSEPEHGALYLDNARLKKG
                     DTFTQENVDAGKFVFSAEELDYQGGDGFAFLVSDISNLFIGPENFIIDIRESYPSGTS
                     HINMDAMVDLVPNPTSGLIDLKCVGMDLKEDVEIQILDIRGVSVMRPFKAQLNLGNRI
                     DLTSLSSGVYLIVVQSGNYIAKKRLVKI"
     gene            2209..2997
                     /locus_tag="PLG24_05690"
     CDS             2209..2997
                     /locus_tag="PLG24_05690"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HMZ24415.1"
                     /translation="MFRSKYFWTHVGLMILAVGLLTGLIFFFLGLYTHHGEKIPISGL
                     NGLQVELAKRQAEEDGFEVVVTDSVFIVGQKGGIVLAQNPEKGTFAKEGRKIYVTLSK
                     YMPDMVPIGSLPALYGKNFELKRKVLMEGYEIQSAVVGQLYDAGEPGCILRVIYNNDT
                     IIDARGVRDNVNLPKGAKLKFIVSTNQGGQMEVPDLICQEFDEARFSLGNNFELSGRS
                     GTSGKYIVAQEPAYTPGVKIDRGTTIIVDLSDSRPPGCPPDEDE"
     gene            3042..5225
                     /locus_tag="PLG24_05695"
     CDS             3042..5225
                     /locus_tag="PLG24_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ24416.1"
                     /translation="MNRFSLRIFFFLWILLTGVSSGAQVVYYGLTENPVLFPEKQVSG
                     QRNLISDSRVVVIPSDSQTLCINRPEIGTFDTLILDQASVNPGIDVKITENCIVFIAE
                     QNAELGDYEYTFDYSSFSGNSLKYRVALSVVEPRGLPFFDDFAYNDKYPDQRRWVDKN
                     VFINNTMGDNPPSVGVATFDGLNSNGSPYGGGFGRADYLTSTFFDLSGVQHSEPVFLS
                     YYLQPKGKTYNHQLRDSIELEFKNKSGQWERIVAYKGIDPSFPSSYVPPFEYYSVQVD
                     PKYFYRGFQFRFVNYNYRLGVYSTWHLDYVKLIANQLPGLNQQDIAFTDPPNRILRTY
                     SAIPYRQLSGFEDKELADTTKIRLYNHFLEVAEDLGDPRLEIQEITTGTEIYNGQLVN
                     TVPQLNPPAGFNAFDNPFDPQSIAASVASVPAGQLPLIFRTKYHFDQDQEVSFLEENN
                     TVTSEAEVGYVLAYDDGSAELNIAAEANNSVKSQIAVKFRLNTGDTLRAVQFHFPRLY
                     DDVSHQLFNLKIWVGSLKEEPDYYYQLQRPVYADTYYDTLQGFTTYPLVNDLTQEPLP
                     LYIPPGDFYIGWQQFSVSSTGQFIPVGFDRNYQGGEALTYYKSTGDWKPMTELTASPL
                     LKGIPMIRAKFQDAWKTSSTTQSKATGFYVFPNPVSSVLNIGASEEMSDNATYHIIDI
                     TGKVVMAGDVIKPIDTGGLSEGIYFLLVRNKEGKNLGNARFVKVR"
     gene            complement(5559..5843)
                     /locus_tag="PLG24_05700"
     CDS             complement(5559..5843)
                     /locus_tag="PLG24_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24417.1"
                     /translation="MPLEGSAFYANIMALYHGIAGLNKIQILSKKPFILRIDKHTQKL
                     LEKWAADEFRSLNGQIEYLLHRALVLSHRWKEEEIPPEKEEPTNDNSNCD"
     gene            complement(5868..6734)
                     /locus_tag="PLG24_05705"
     CDS             complement(5868..6734)
                     /locus_tag="PLG24_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMZ24418.1"
                     /translation="MKGEYYIKPKNGFLFLLILFLLLGATILLPVMFGDSAISLFAIV
                     PGILFLLVLPGFKVIYPNEAIAFTFFGKYAGTAKENGFFWVNPFYLSKKLTLRARNLN
                     GDRLKVNDGVGNPIEIAAVIVWQVADTFKAAFEVDNYVNYVMVQSEAAVRALANKYPY
                     DQFEDENAQLTLLSGAEKVNDELERALHERLDQAGIRIIEARISHLAYAPEIAGAMLQ
                     RQQAIAIVAARQKIVEGAVGMVDLALTKIKEGSIIDLDDHTKAAMVSNLMVVLCSDRA
                     AQPIINTGTLNH"
     gene            complement(7266..7643)
                     /locus_tag="PLG24_05710"
     CDS             complement(7266..7643)
                     /locus_tag="PLG24_05710"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HMZ24419.1"
                     /translation="MVRTLALFYFLLLTTLVFGQQRITTAIRNGDAKALSGFFNKQVE
                     LTFDDRGDLMTRAEAERMLESFFKTNKVISYTPSHSGKAPDHSGSYTLGNIRTENRHF
                     RIFIKFRPGLGLDSIREVRINSL"
BASE COUNT         2283 a         1664 c         1790 g         2261 t
ORIGIN      
        1 ggcggtcaca ccttcaatgc ttgcaatgga agcacccagt ataacccgtt ttcttctttc
       61 gaaccgggag ccgggagcac gatactgtca tatggagaca attgtggatc agataatgtc
      121 ggcgaaaggg tggattattt tcatgtagca aatattattg aagtcaatcg ttatgtcaag
      181 gaaatagaga acaaatgcgg aacatgggga gcggagatca atcatacacc cgtaggctac
      241 ctttcaatgc ccgaagacaa aacaattcca atccttactc cattcgaact ggagggaggg
      301 gcgacagatg atgataagaa tcttacattc aactgggagc aatacgatct gggcgaagga
      361 gagcccctgg gcaccaattt tgaaacagga cccttgtttg tgtctcaggc tcctggcggt
      421 gtagggcaca gattattccc ttccttgtcc acaattaatt ccggaattat ttccaatcgg
      481 gagcgcttgc cggaggtatc aagggagatg aatttcaaaa tgaccgtgcg cgacaataat
      541 ccaaatgttg gggcccaagc tatcgtgtcc tataagttcg atgtaacgga tcaggcagga
      601 ccctttaagg tgacatttcc ggatgcccag acagatacta gttttacttt gggacagtat
      661 gttgagatta cctgggatgt cgctaataca gatcaatttc cggtcagttg caaaaatgta
      721 aatattctct ttagcactac caatggcaat acatggtctg atacattggt tgccaatacc
      781 ccgaatgacg gcagagaatc ggtaatgatg cccaggactt caggacgggc aagatttaag
      841 gtaaaggcag ctgacaacat ctttctggac ctttccggaa aacccttgaa aatagtgcaa
      901 ccttcagaag cgggttatag tctggatatc ctgccccatg atatatcctt gtgcaaaaac
      961 agtatttaca cgcttactgt aaagtctgtc agttggaaag cttttaattc tcctgtaaag
     1021 atttcggtgt ttgattcagg acacccagct tttaaggtca ttcctctaaa aagtgaattt
     1081 ctgccgggag agacaatgac cctggacgtc gatgtgccgg atgtcataga tgccggaacc
     1141 tatcagatcc ggtttatggc tacttcagat caaggcgata cacttttcag aacagttaca
     1201 ttgaccattg cggacaacga tcaaaacatt tccggaacca tcagtccggt tgccaacgat
     1261 cagtacgtaa cgcttcatcc ggtttttgcc tgggatcccg tggaaaatac ccatggctat
     1321 caattacaat tggcgacgga tccggccttt tcatctctga tcctgaatgt gtcaatagac
     1381 tcagaggagt atttatccga aatagaactc aataaagcga ccatctacta ttggcgggtc
     1441 cgctctttgg gggaatgcgg ttatggtcgc tggacggcaa tgagtatttt caggacagag
     1501 tccgaattgt caaacagcgc actacatcag atcaataata agatcttaaa agtcaagact
     1561 aatgagaaaa agccggcctt acatgaagat ttgtactacg tatcagaatt gaatcagaac
     1621 gaatcactga aatataccct gctttcagag ccggagcacg gcgccttgta tctggataac
     1681 gcacgcttaa aaaaaggaga tacatttact caggaaaatg tcgatgccgg aaaatttgtt
     1741 ttctctgcag aggaattgga ttatcaagga ggagatggat ttgcctttct ggtgtctgat
     1801 atttccaatc tcttcatagg accggagaat tttataatcg atatccggga aagttatcca
     1861 tccggaacat ctcatatcaa tatggatgcc atggttgatc tggtgcccaa tcctacttcc
     1921 ggactgatag atttgaaatg tgttggaatg gacctgaagg aggatgtgga gattcagatc
     1981 ttagacatcc gtggagtatc agtgatgaga ccgtttaagg cccaattaaa tttggggaac
     2041 cgcatagacc tgacttcttt gtcttcaggc gtctatttga ttgtggtaca atcgggaaat
     2101 tatattgcca aaaaaaggct cgtcaaaatc taatcaacta tatttgccgg gtaaaatcca
     2161 cattgactga cttacgttag cgttataatg tggtaacatc gttgtattat gttccggtcg
     2221 aagtatttct ggactcatgt tggtttgatg attctggctg ttggtctgct gacagggctg
     2281 atttttttct ttttaggtct atatacccac catggagaga aaatcccgat atccggacta
     2341 aatgggcttc aggtcgagtt ggcaaaacgg caagctgaag aagatggatt tgaggtagtt
     2401 gtcaccgatt cggtttttat cgtcggacaa aagggcggta ttgttcttgc acaaaatcca
     2461 gaaaagggaa cctttgccaa ggagggtaga aagatttatg tcacgctatc caagtatatg
     2521 ccggacatgg tgcccatcgg ttcactccct gcattgtacg gaaagaattt cgaattgaaa
     2581 agaaaggttt tgatggaggg gtacgagata caatcggcag ttgtaggcca attgtatgat
     2641 gccggggagc cgggctgtat cctccgggtg atatacaata atgatacaat cattgatgcg
     2701 aggggagtac gggacaatgt caatcttccc aaaggcgcca aattgaagtt tatcgtatcc
     2761 accaaccagg gagggcagat ggaggtgccg gatcttatct gtcaagagtt tgatgaggca
     2821 aggttttctt taggaaataa ttttgaattg tccggacgtt ccggcacatc aggcaaatat
     2881 atagtggcac aggaaccggc atatactcca ggtgtgaaga tcgacagagg gacaacaatc
     2941 atcgtcgatc tgagtgattc acgtcctccc ggatgcccgc ctgatgaaga tgaatagcaa
     3001 caaataagac agacatttcg tttttaaata aaaaatatcg aatgaatcgt ttttcactga
     3061 ggattttctt tttcttatgg atattgctga ccggtgtaag tagcggcgct caggtcgtct
     3121 attatggatt gaccgaaaac cctgttctgt tcccagaaaa gcaagtatct ggacagagaa
     3181 atttaatcag tgacagtaga gtggttgtca taccctccga tagtcagaca ctctgcatca
     3241 atcgtccgga gataggaacc tttgatacac tgatccttga tcaagcctcc gtcaatcctg
     3301 gtattgatgt gaaaataacg gagaattgca ttgtatttat tgcagaacaa aacgcagaat
     3361 taggggatta tgaatacacc tttgactatt cgtccttttc cggcaactcc ctgaaatata
     3421 gggttgcatt gtcggttgtg gagccgagag gattgccatt cttcgatgat tttgcctaca
     3481 acgacaagta tcctgatcaa cgaagatggg tcgacaaaaa tgtattcatc aacaatacaa
     3541 tgggcgacaa tcctccaagt gtgggtgttg caacatttga cgggttgaac agcaatggaa
     3601 gcccttatgg tggtggattc ggtcgggccg actacctcac ttccacattt tttgatctaa
     3661 gtggagtaca acattctgaa ccggtttttc tgagctatta tctgcaaccc aaaggaaaga
     3721 cgtataatca tcagctgcgt gatagcattg agctggagtt taaaaacaaa tcaggtcaat
     3781 gggaaaggat tgtagcatac aaaggtattg atccttcctt tccatcctct tatgttccgc
     3841 cttttgaata ttacagtgtc caggtcgatc caaaatattt ttacagggga tttcaattta
     3901 ggtttgttaa ttacaattac cggcttgggg tatatagtac atggcacctg gattatgtca
     3961 aacttatcgc caatcagttg ccgggcctta accaacagga tatcgccttt acagatccac
     4021 ccaacaggat attaaggact tattcggcga taccttaccg gcagctgtcc ggtttcgagg
     4081 ataaggagtt ggcagataca acaaaaatca ggctctacaa tcatttcctg gaagttgctg
     4141 aagatttggg tgatccacgg ctggaaatac aggaaataac tactggtacg gaaatataca
     4201 atggacagct tgtcaataca gtgccacagc tgaacccacc cgccggcttc aatgcttttg
     4261 acaatccctt cgacccacaa tcgattgccg cttctgtggc ttctgttccc gcaggtcaat
     4321 tgcctttgat tttcaggact aaataccatt ttgatcagga ccaggaagta tcctttctcg
     4381 aagaaaacaa taccgtcact tccgaagcag aagtcggata tgttctggca tacgatgatg
     4441 gcagtgctga gctcaacatc gccgccgagg ccaacaacag tgtaaaatca cagattgcgg
     4501 tcaagtttcg cttaaataca ggagatacac ttcgtgccgt tcaatttcat tttcctagat
     4561 tatatgatga tgtctcccat cagttgttca atcttaagat ctgggtcggc agcttgaaag
     4621 aagagccgga ttattactat cagctgcaac gtccggtcta tgcggataca tactacgata
     4681 cgcttcaggg atttacaacc tatccgctgg tcaatgatct gacccaggag cctttgccac
     4741 tctatattcc accgggtgat ttttacatag gctggcaaca attttctgtt tcttccaccg
     4801 ggcagtttat cccggtcggg tttgacagga attatcaggg aggagaagcc ttgacctatt
     4861 acaagtcgac cggagattgg aaaccgatga ccgagctgac agcatcgccg ctgctcaagg
     4921 gcattcccat gattcgggca aaatttcaag atgcctggaa aacatcttcc acgacacagt
     4981 ccaaagcaac tggtttttat gtttttccca atcctgtctc aagtgtactt aatataggcg
     5041 caagtgaaga aatgtccgat aatgccacat accacattat agatattaca ggaaaagttg
     5101 ttatggcagg tgacgtcata aagccaatag ataccggggg gttgtccgaa ggtatttact
     5161 tcctgctcgt caggaataaa gagggaaaaa atctcggcaa tgcaaggttt gtcaaggtcc
     5221 ggtaattaca gtttgtaaat atttagtggg tttttggctc tcaaagtcaa caaaatacgg
     5281 caaggtggtt ggtaaagact tgcaaaaaac gaactaggta gatgattaat aggctgaata
     5341 tcagtggata ccggatctct gtataatttc attgtaaaaa ccacctcgaa atgacatttt
     5401 ttttggagca ttctctgcag aagctccata gcgtcaggcg aagtgattat tatttatcta
     5461 cccacttcga aactaccgct caacaacctc ttccccaccc ttgtgaatat agtaaaccgg
     5521 gaattccccc cgcagaggaa ctcttgcgcc atggacagtt aatcacaatt tgaattgtcg
     5581 ttggttggct cttccttttc agggggaatc tcttcttcct tccaacgatg tgacaggacc
     5641 agggctctat gcaacagata ttcgatctgg ccgtttaggc tacggaattc gtccgcagcc
     5701 catttttcca gaagcttttg cgtatgctta tctatcctta atatgaatgg ctttttactc
     5761 aagatctgaa ttttatttaa cccggcaata ccatggtaaa gcgccatgat atttgcatag
     5821 aaggcgctgc cctctaaagg cattgccggg ttggggtatg gttaaaatca atgattcagc
     5881 gtgcctgtat tgatgatagg ctgagcggca cggtcggaac acaaaacgac catcaggttg
     5941 ctgaccatcg cggctttcgt atgatcgtcc agatcaataa tgctgccctc cttaatcttg
     6001 gttagcgcca aatctaccat accaacagct ccttctacaa ttttttgcct tgcggcaaca
     6061 atggcaatcg cctgttgtcg ctgcaacata gctcccgcta tctccggagc ataggcaagg
     6121 tggctgattc ttgcttctat gattctgatg ccggcctggt ccagcctctc gtgcaaggct
     6181 cgctcaagtt cgtcattgac cttttctgct ccggaaagta gtgttaattg agcgttttcg
     6241 tcctcaaact ggtcatatgg atatttgttt gccagggctc gtacagcggc ttcactttgg
     6301 accatgacat aattgacata attgtctact tcgaatgcgg ctttgaaagt atcagccact
     6361 tgccatacaa taacagcagc aatttcaatt ggattgccga ccccgtcatt gacttttagt
     6421 ctgtctccat tcaggttgcg tgctcgtagt gtgagctttt tactcaagta aaacgggttg
     6481 acccaaaaaa aaccgttctc cttggcggtc cctgcatact tcccaaaaaa agtaaaagca
     6541 attgcttcat tagggtaaat gaccttaaat cccggcagga caagcaaaaa caatatgccc
     6601 ggaacaatag caaataatga aatggcagaa tcaccgaaca tgacaggaag tagtattgtc
     6661 gctcccaaca ataggaacaa aatcaacagg aacaggaatc cgttcttggg ctttatataa
     6721 tattcgcctt tcatggtttc aaatgtttat ttagtgatag attattaaaa tgatatcatt
     6781 ataatatcaa attgaaattt ggggtcaaaa gttccaatcg tgttgtcttt ttttaaaaaa
     6841 tgtataggaa tctgtaaaaa attgacatat accaatgatg gaatatttgt ttttatagca
     6901 tattctttgt tgttcaagag ttgtttatag ccgcatgaac atttgattaa gattcgttaa
     6961 tatttattct ttttgggatc aaaaaaatgc agggccaacc agttatattt cctcttttct
     7021 gaacgtatgt ccgaaatcag acgaaagata acccatccct tgacctgctt tgacctgccc
     7081 ggttttctgt ttgggtcatg taggcaatga agttggtcaa atgatgtagt acggacccac
     7141 ttgaaagaag atcctctatt aatcaaatct cagagtcttc gggtattaca gctgcatttt
     7201 tgggtgcatc gtgaaaaacc aattaccgga aatagttatt taagtagtgg aaggcctcgt
     7261 ttttgtcaca aggaattgat gcggacctct ctgatggaat ccaaacctaa gcctggacgg
     7321 aacttaatga atatcctgaa gtgacggttt tctgttctga tatttccaag ggtataactg
     7381 ccggaatgat cgggggcttt tccggagtga gatggagtat aggaaatgac cttgttggtc
     7441 ttgaagaaag actcgagcat cctttctgct tcggcgcgag tcataagatc tccccgatca
     7501 tcaaaagtca attcaacttg cttattaaaa aaaccggaca gggccttagc atcaccattc
     7561 ctgatggcag tcgtgatacg ctgctgcccg aacacaagtg tagtcaggag caaaaaataa
     7621 aacaaagcaa gtgttctaac cattttttgt gattctcttt agatatataa cacaaatttt
     7681 atgccaatat tataatagga gaaaactttg ttttattgaa taagtggaaa tttttaggaa
     7741 aatattcgat gacgcgaagg cccccgggag aagaggttgc tttgttttaa tactgcaaat
     7801 caatcattgg atgcctgtat ttaggggtac agactcaaga taataattag gtaatagatt
     7861 cccggagcca tgttataatc ctacaatcat gaataacatc cgccatcccg cctcatcaca
     7921 gatgacgcaa tatttttcct gcaaaatccg gccaatttta gtggatttga cagtttctga
     7981 taaaagctga ttattttt
//
LOCUS       DAOQQN010000114         7985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3564_length_7985_cov_5.256368, whole genome shotgun sequence.
ACCESSION   DAOQQN010000114 DAOQQN010000000
VERSION     DAOQQN010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7985
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3564_length_7985_cov_5.256368"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PLG24_05715"
     CDS             complement(<1..338)
                     /locus_tag="PLG24_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24420.1"
                     /translation="MKKNRIILNTGLGLIFLAGVLISVMSGKTPVKKIKPIYQTQQQW
                     VDSIMKTMTMEEKIGQLFMVRAHSDKGPDHIRFIKHLISTYKIGGLCFFQGTAEKQVD
                     LTNEYQKLASP"
     gene            complement(335..2329)
                     /locus_tag="PLG24_05720"
     CDS             complement(335..2329)
                     /locus_tag="PLG24_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24421.1"
                     /translation="MAKFNKSERVKTQKVITAPFPGSDADKLGNLIPTRLMPFLIVVF
                     AILFYANSLGGSYVMDDAIVISENQFTIQGVAGWKGIFSHDTFYGFFRESGKNHLVSG
                     GRYRPLSLALFALEGQVFGFKPFVFHLFNLLYYALTCFLLFRLFHYLFESRYGRTNAT
                     LIAFFCSLLFTVHPIHTEVVANIKGRDEILTCLLGLAGTWYFIRSIDRGRLALSILAA
                     FLWFLSLLAKENGIVFIALAPLAVWFFKDVKMSDILRYSIPAVVMTILYLILRRTVLG
                     PAPDSGPIMELMNNPFLTWHINGYLPVTFSIKLATIAVTMLKYILLLIYPYQLTSDYY
                     PQQIPLSDWSNWRAILGFLLYATAIVICLIRLKKKEIITFGILFYLVALFPMSNILFP
                     VGTLMSERFLFIPSIGFALIISRLIHIYVVKGSLNSRLNLLILALILILSVKTIARNK
                     DWSDNYTLFTTDVKISDNSAKMLNAAGGALLDSCIRVKDPIVKKQMAEEAQRYLLRAI
                     ELHPSYSNAYLLLGNSYAYTDNLEKAAENYQLALQYNQNFMDAKINLASMYKELGRKA
                     GEKEGNLPKAKEMLGKSLQLNPKDPETNRLLGIAIGMSGDPKGALIYFKAALEAKPDD
                     AFYMFDLGSAYANAGDMEKANYYHAEAIKKDPSLQQRLNK"
     gene            2499..3065
                     /locus_tag="PLG24_05725"
     CDS             2499..3065
                     /locus_tag="PLG24_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HMZ24422.1"
                     /translation="MTRFVVFCCLLSMPIMSFAQLTVSPKAGINYLWVGSRPQGFDRA
                     GAEQGWQAGLDLRLGKRFYFQPGVYYVSTGATISSLEDIKNIKTGNASFMKIPVNVGY
                     KLINSQLFKLRVHGGGTANKLLATRDGGALSASDFEPWNYGANVGAGIDLLFLALDIN
                     YEWGLTNMYSNNKNSLPTMASISVGLKF"
     gene            3210..5708
                     /locus_tag="PLG24_05730"
     CDS             3210..5708
                     /locus_tag="PLG24_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HMZ24423.1"
                     /translation="MTVTSQAQWKKKSKEEAKPKVTAPADTSAKKSKDGLQPYDKVIG
                     KDAVSRWGLFGVHNVKDKWYFEINDTILNRSMLAITRYVKTAAGGGIYGGEQIKKLML
                     HWEKGPGDKIFMRGTTLIVNSPDSSKPIFQAVQNSNSEPIVGSFSVKSIKKGDKGNSY
                     VIDVTDFFNGDEHSFSLGPINKQRFKLKSLQKNSSFINKISSYPINVEIRTTKTYDIV
                     PPSMDDRSAPSTGSYLPSGGDAGFVTMELNTSMILLPEKPMRKRSFDSRVGFFSGQWT
                     VYGEESQRSNLDTYVTRWRLEPKNASDAEKQLRGELIEPAKPIVYYIDPATPDKWKPY
                     LKQGVDDWNAAFETAGWKNAIRGEYWPENDTTMSLEDARFSVIRYFASDIQNAYGPNV
                     HDPRSGEILESHIGWYHNIMQLLRNWYLVQAGATDPKAGKREFDDELMGRLIRFVSSH
                     EVGHTLGLRHNMGASYATPVEKLRDKDWTTKNGHTSSIMDYARFNYVAQPEDGVTNLY
                     PQIGDYDKWAIKWGYSNVGGKDDKEDAVILNNMIKEAYKNPRLRFGTEISQYDPRYQT
                     EDLGDNAMKASAYGIKNLQRILPKLIEWSVKDGESYTQLEEMYESLVGQFRRYMGHVA
                     KNVGGVYDDPTTSDMDVQPFRPVPKSIQKEAVAFLNQNLFTAPLWLVDQNIISKIRPN
                     AGVDAINKIQTSTLHNVLSGDKLIRLMEFSGTTGYSVEDLLNDLEANIIQKQSGVDIY
                     QRNLQKAYVSKLISLLNPGSASSVELKIGTSYGSSPMTVNLNDTDVPSIARGHLEKIR
                     QTAKLRADTAANSSVRYHYKDILARIDKALKGDK"
     gene            5870..6478
                     /locus_tag="PLG24_05735"
     CDS             5870..6478
                     /locus_tag="PLG24_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HMZ24424.1"
                     /translation="MIDTIVFDLGGVLIDWNPMYVYKDYFPSKEDEQYFFEKVATFEW
                     NEEQDAGKPLIQATEERIRLFPEWEQPLRDFYGRWEEMLKGPIRDTVEIFRSLKETQA
                     YRFYALSNWSAENFHVALDRFEFLHWFDGRLISGEEKMAKPNPAFFKLLETRYDVIPQ
                     NTVYIDDNFRNYKAAEALGFLAIHFISPEQLKGELGRYSIEI"
     assembly_gap    6826..6925
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6926..7109
                     /locus_tag="PLG24_05740"
     CDS             <6926..7109
                     /locus_tag="PLG24_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24425.1"
                     /translation="SSTKVLTYYFPTHFGTICVFSRLQRRIHLSLYPDLLRQPFSILA
                     NSPYIRQTNMFLPVCV"
     gene            7100..>7985
                     /locus_tag="PLG24_05745"
     CDS             7100..>7985
                     /locus_tag="PLG24_05745"
                     /inference="COORDINATES: protein motif:HMM:NF037611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24426.1"
                     /translation="MRLIPVLLLIFGISVYAQQKTAYDYVDPMIGTQRMGHVYPGATV
                     PFGMVQLSPDTDTIPYSVDGRYNPDVYKYCAGYQYGDKTIVGFSHTHFSGTGHSDLGD
                     FLIMPTQGPLQLNPGIASNPKSGYRSGFSHATEMARPGYYKVDLADHDITAEMTASER
                     VGVHRYTFHKAGLSHIILDLMHGIYNYDDKNIWTFVQVKDDYTVVGYRQTKGWARTRL
                     VYFAMQFSKPIKNYGCVNYDKREVYRGFWGKFDQEHIFPELAGKKLRVYFDFDMAQDE
                     ALEIQVALSAVGTEGAMAN"
BASE COUNT         2259 a         1679 c         1845 g         2102 t
ORIGIN      
        1 ggtgaagcaa gtttttggta ttcattggtc aggtccactt gcttttcggc agttccttga
       61 aagaagcaaa ggccaccgat cttgtaagtg ctgatgagat gtttgataaa gcgtatatgg
      121 tcgggacctt tatccgaatg cgctctcacc ataaacagct ggccaatctt ttcttccatc
      181 gtcattgtct tcatgatgga atcaacccat tgttgttgtg tctgatatat tggtttgatt
      241 tttttcacag gagttttgcc tgacatgacg cttatcagga cgcctgccag gaagatcagg
      301 ccgaggcctg tatttagtat aatccgattt tttttcactt attcaatctt tgctgcaagg
      361 acggatcttt tttgattgcc tctgcatgat agtaatttgc cttttccatg tctccggcat
      421 tggcgtatgc actaccgagg tcaaacatgt aaaaggcgtc atcaggtttt gcttcaagag
      481 cagccttaaa ataaattaaa gcaccctttg gatcgcctga cattccgatc gctattccca
      541 acaatctgtt ggtctcagga tctttaggat taagttgcag cgatttgccg agcatttctt
      601 tagcttttgg cagattgccc tccttctcac ctgcctttct gcccaattcc ttgtacatac
      661 ttgccagatt gatttttgca tccataaaat tctgattgta ttgcaatgca agctgataat
      721 tttccgctgc tttttcaaga ttgtctgtgt aggcgtagga attacccagc agcagatagg
      781 cattgctgta tgaaggatgg agctcaatcg cgcgtagaag atagcgctga gcctcctctg
      841 ccatctgttt cttcacaatc gggtccttca ctctgataca gctatccagt aatgcgccac
      901 ccgcagcatt gagcatttta gcactgttgt cggatatctt gacatcggta gtaaacagcg
      961 tataattgtc actccaatct ttattgcgag ctattgtttt aacgctcaaa attaggatta
     1021 aagccagaat taaaagattg agacgggaat ttaaacttcc cttaacaaca tagatatgta
     1081 tcaatcggct gattatcaat gcaaaaccaa tggatggaat gaaaaggaaa cgttctgaca
     1141 tgagtgttcc caccggaaag agaatatttg acattggaaa caaggcaacc agatagaaaa
     1201 gtatgccaaa tgtgatgatt tccttctttt tcaatctgat cagacagatg acaatggcag
     1261 tggcatacaa tagaaaaccc agaattgcac gccagttgga ccaatcggac aatggaattt
     1321 gttgaggata ataatcactg gtcaattgat atgggtagat cagcagtaag atatatttga
     1381 gcatagttac tgctatggtg gcgagtttta ttgaaaatgt aaccggcagg tagccattga
     1441 tatgccaggt aaggaagggg ttattcatca attccatgat gggaccgcta tccggagccg
     1501 ggcccaaaac tgttcttcga aggatgaggt aaaggatggt catgacaact gcgggaatgc
     1561 tgtatcttag gatgtcagac atttttacat ctttaaaaaa ccacacggca agtggagcta
     1621 acgcgatgaa aacgatgccg ttttccttag ccagcaagga gagaaaccat aaaaaagccg
     1681 cgagaatgga aagagctaac cgtcccctgt caatgcttcg aataaaatac cacgtccctg
     1741 caagacccag cagacaggtc agtatctcat cccgaccttt gatattggca actacttctg
     1801 tgtgtatagg atggacggta aaaagtaggg agcagaagaa ggcaatcagc gtggcattgg
     1861 tccggccata tctgctttca aaaagataat ggaatagcct gaacaataaa aagcaggtca
     1921 gagcataata taacaggttg aaaagatgaa agacaaaggg cttgaagccg aatacctgtc
     1981 cctccagggc aaacaaggca agggaaagag gccggtatct tccccctgac accaaatggt
     2041 tttttccact ttccctgaag aagccgtaaa aggtatcgtg gctgaagatg cctttccatc
     2101 ctgcaacacc ctggatagta aattgatttt cagaaataac gatggcatcg tccatgacat
     2161 aggatccgcc taaactgttg gcataaaata gtatggcaaa aacaacaatc aaaaaaggca
     2221 tcagtctggt gggaatcaaa ttgcccaatt tgtctgcatc tgatccggga aagggagcag
     2281 ttataacctt ttgggttttg actcgttcgg atttgttaaa ttttgccatg attgcccgtt
     2341 gaaaattaag gtttctctta attaaatttt aaaaaattaa aaaatacagc agttacaaaa
     2401 ataattattt cacttgaatc gcttaaaaaa tgtgcgactg gataaccaca atatcaggtg
     2461 catccgacga tgatttatta acctaaaaaa tgcagatcat gacacgattc gtagtatttt
     2521 gttgcctttt gagcatgccg attatgagct tcgcccaatt aacagtgagt cctaaagccg
     2581 gaatcaatta tctctgggtc ggaagcagac cacagggttt tgaccgggcc ggtgcagaac
     2641 agggctggca ggcaggtctg gatctgcgac tcggcaagcg gttttatttc caacccggtg
     2701 tctactatgt aagtaccgga gctactattt cctctctgga ggatatcaaa aacatcaaga
     2761 ccgggaatgc ctctttcatg aaaatacccg tcaatgtagg atataaactg attaattctc
     2821 aattgttcaa gctcagggtt cacggtggtg ggacagcaaa caaactcctg gcaaccaggg
     2881 atgggggtgc tctttctgcg tcagattttg agccctggaa ttacggtgca aacgtaggtg
     2941 ccggcatcga cctgttgttt ttggctctcg atatcaacta tgaatggggc ctgaccaata
     3001 tgtattcaaa caataaaaac agcctgccga cgatggcaag tatttctgtt ggattaaagt
     3061 tctgacaaga ctcgagcaga aggtgtttca aatagccgtg gtagtgtaat gcctcggcta
     3121 ttttttattt caggtaaaaa tccttagttt tacccgaaag agtaaattca atgatgacac
     3181 gttatttttt attgattagt ttttgcttga tgactgtgac gtcgcaagcg caatggaaga
     3241 aaaaatccaa agaggaggct aagcccaagg tgactgcacc tgctgatact tccgcgaaga
     3301 aatctaagga cggattgcag ccttatgaca aggtaatagg aaaggatgcc gtgagtcgct
     3361 ggggcttgtt tggggtgcat aatgtcaagg acaaatggta ttttgaaatc aatgatacca
     3421 tactcaaccg gtccatgtta gccattacca ggtatgtcaa gacagctgcc ggtggaggta
     3481 tatacggagg agagcagatc aagaagctta tgttgcattg ggaaaagggt cccggagata
     3541 agatctttat gcggggtacg accctgatag tcaacagtcc tgacagctca aagcctatat
     3601 tccaggcagt ccagaattct aacagtgaac cgattgtagg ttcgttttca gtcaaatcca
     3661 tcaaaaaggg agataagggc aattcgtatg tcattgacgt gactgacttt ttcaatgggg
     3721 acgaacatag ttttagtctt ggaccaatca ataagcaacg gtttaagcta aaaagcctgc
     3781 aaaaaaattc ctccttcatc aacaaaattt cttcatatcc catcaatgtt gagatcagaa
     3841 cgaccaaaac ttatgatatc gtgccgccct ctatggacga tcgctcagcg ccttccactg
     3901 gcagctattt accttcagga ggagatgctg gttttgttac catggagttg aacacatcca
     3961 tgatactttt accggagaag cccatgagga agagaagttt tgatagtcgg gtaggcttct
     4021 tttccggcca atggacagtc tatggagaag aatcccagcg atcaaactta gatacctatg
     4081 ttacacgctg gcgtcttgag cccaaaaatg cctctgatgc tgaaaaacaa ctacgcggag
     4141 aactgattga gcctgctaag cccatcgttt actatataga tccggctaca cctgataagt
     4201 ggaaacctta tctgaagcag ggcgtcgatg attggaatgc ggcatttgag acagcaggct
     4261 ggaagaatgc catccgcgga gagtattggc cggaaaatga tacgacaatg agccttgaag
     4321 atgcacgttt ttccgtcatc agatatttcg catccgatat ccagaatgct tatggaccta
     4381 atgttcacga cccacgcagc ggagagatac tggaaagcca catcggctgg tatcacaata
     4441 tcatgcagct gttgcgcaac tggtacctgg ttcaggccgg ggccaccgat cccaaagcag
     4501 gcaagcgtga gtttgatgac gagttgatgg gtcggcttat ccgatttgtt tcatcacacg
     4561 aagttggtca cacgctcggt ctgcgacata atatgggggc aagttatgcc acacccgttg
     4621 agaaattgcg agacaaagac tggacaacaa aaaatgggca tacttcctcg atcatggact
     4681 atgctcggtt caattatgtc gctcaaccgg aagacggtgt caccaactta tatccacaga
     4741 tcggagacta tgacaagtgg gcgatcaaat ggggttacag taatgtaggg ggcaaggatg
     4801 acaaggaaga cgctgtcatc ctcaacaaca tgattaagga agcatacaaa aacccgagac
     4861 tacgattcgg tacagaaata agccagtacg atccacgcta tcagacagaa gacctggggg
     4921 ataacgccat gaaagcctct gcctacggca taaagaactt acagcgcatc ctgcccaaat
     4981 tgatcgagtg gagtgtaaag gatggcgaaa gttatacaca acttgaagaa atgtacgaat
     5041 cactggtagg ccagttcagg agatatatgg gacatgtggc aaaaaatgtc ggcggtgtgt
     5101 atgatgatcc gacgacaagt gatatggacg tccagccatt caggccggta ccaaagtcaa
     5161 tccagaagga agcggttgca ttcctcaatc aaaacctgtt tacagcacca ttatggctgg
     5221 tcgatcagaa cataatttcc aagattagac caaatgcagg tgttgatgcc atcaataaaa
     5281 tccagacatc gacactccac aacgtgcttt ccggtgacaa gctcatccgg ttgatggaat
     5341 tctcagggac aaccggttat tcggtagaag atttactcaa tgatcttgag gcgaatatca
     5401 tccagaaaca aagtggagtc gacatctatc aacgtaactt acaaaaggca tatgtatcaa
     5461 aactgatctc cctgctcaac ccgggatcgg cctcatccgt agagttgaag ataggaacca
     5521 gttacggctc gagccctatg actgtcaatc tgaatgatac cgatgtgcct tccatcgcac
     5581 gcgggcattt agagaaaatc aggcaaacgg ctaaacttag ggctgacaca gcagcgaatt
     5641 cttcggtcag gtatcattat aaggatattc tggcgcgaat agataaggct ttgaaaggcg
     5701 ataaataatt aatgaaaggc cggtcgggag atccggcttt tttaccactt gtatttccgt
     5761 aggcgttggc atcaaaggga caacctttgt cttcggctgc cgttgtatac gtagtttcga
     5821 atcaccgaaa acaacatgat tgatgtcctc gtatattaat aattcaaaca tgatagatac
     5881 gatagtcttt gaccttggcg gtgtcctgat agactggaat cccatgtatg tctataaaga
     5941 ttattttcct tcaaaggagg atgagcaata tttttttgaa aaggtcgcca cctttgagtg
     6001 gaatgaagaa caggatgcgg gtaagccact gatacaagct actgaagagc gtatccgact
     6061 gtttccggaa tgggagcagc ctttgcgtga tttttatggc cgatgggagg agatgcttaa
     6121 ggggcctatc cgggatactg tggagatatt caggtcgctg aaggaaacgc aagcctatcg
     6181 gttctatgca ttgagcaatt ggagtgcaga aaatttccat gtcgctcttg acaggtttga
     6241 atttttgcat tggtttgacg gcaggctaat cagtggggaa gaaaagatgg caaagccaaa
     6301 tccggctttt ttcaaactac tcgaaacaag gtatgatgtc atccctcaga atacggtcta
     6361 tatcgatgac aatttcagga actataaggc tgcggaggcc ttaggtttcc tggcgattca
     6421 tttcatttcg ccggagcagt taaaggggga gttagggaga tattcgattg aaatatgagt
     6481 atttcggatg cccgacctga agtaggtaga tgattaatag ccgagtgttc cctgttggtg
     6541 tccatgtggt gataatgtga ttgtcctgat gttacaatat tgcggaaatt gatttgctaa
     6601 tagtctgaaa agacaagcag cacaaaggtt ctgacttatt attttcctac ccacttcggc
     6661 acgatctgca tcttctccct acttcagcgc cgcatccacc ttagccttta tccgggcctc
     6721 ttaagacaac cattctcaat tggggagtta gagaactatt cgattgattt tgagtatttc
     6781 ggatgcccga cctgaagtag gtagatgatt aatagccgag tgttcnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnaagca gcacaaaggt tctgacttat tattttccta
     6961 cccacttcgg cacgatctgc gttttctccc gacttcagcg ccgcatccac cttagccttt
     7021 atccggatct cttaagacaa ccattctcaa ttcttgccaa ctctccttat attcgccaga
     7081 caaatatgtt tttgccggta tgcgtttgat tcctgttctg cttcttatct ttggtatttc
     7141 tgtatatgct caacaaaaaa cagcctatga ctatgtcgat cctatgatcg gcacacagcg
     7201 catgggccat gtatatccag gcgcaacggt gccctttggc atggttcagc taagtccaga
     7261 taccgacacg attccctatt cggttgatgg tcgatacaat cctgatgtat ataaatactg
     7321 tgccggttat caatatggcg ataaaaccat cgtaggcttt agtcatacgc acttctccgg
     7381 taccggtcac tccgacctgg gagattttct gatcatgccg acccagggac ccctgcaact
     7441 caatccaggc attgcatcca atcctaaaag cggctatcgg tccggctttt cccatgcaac
     7501 cgaaatggcc aggccgggat attacaaggt tgacctcgca gatcatgaca taaccgcgga
     7561 gatgacggca agtgaaaggg tcggagtgca cagatacaca ttccataaag ccggattgtc
     7621 ccatatcatt ctcgacctga tgcacggcat ttacaattat gatgacaaaa atatctggac
     7681 ctttgttcag gtaaaggatg actatacggt cgttggatat cgtcagacca agggctgggc
     7741 gcgtacaagg ctggtgtatt ttgccatgca attttccaag ccgatcaaaa actacggctg
     7801 tgtcaattat gataaaagag aggtctatcg gggcttttgg ggcaaattcg accaggaaca
     7861 catcttcccg gagctggcgg gcaaaaaact gagggtatat ttcgactttg atatggcgca
     7921 ggatgaggcc ctggaaattc aggttgccct gtctgccgta ggaaccgaag gcgccatggc
     7981 taatc
//
LOCUS       DAOQQN010000115         7960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3578_length_7960_cov_6.041113, whole genome shotgun sequence.
ACCESSION   DAOQQN010000115 DAOQQN010000000
VERSION     DAOQQN010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3578_length_7960_cov_6.041113"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /gene="grpE"
                     /locus_tag="PLG24_05750"
     CDS             <1..271
                     /gene="grpE"
                     /locus_tag="PLG24_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HMZ24427.1"
                     /translation="KKIVDDVEEKDPFTEGVLLVYNKLFNVLKSKGLQVLESNGQPFD
                     AELHDAISHIPVSDPSLSGKVIDTVERGYLLNDKLIRHAKVVVGA"
     gene            309..1466
                     /gene="dnaJ"
                     /locus_tag="PLG24_05755"
     CDS             309..1466
                     /gene="dnaJ"
                     /locus_tag="PLG24_05755"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HMZ24428.1"
                     /translation="MSKRDYYEVLGVSKGADEAEIKKAYRKVAMEFHPDRNPGDKVAE
                     EKFKEAAEAYEILSNPDKKARYDRLGHAGVDPNFGGGGYGGHGMTMEDIFNNFGSIFE
                     DSPFGSFFGGGQQRRQGGGQRGSNLRIKLKMTLEEINTGVKKKIRVNKQVSCQTCGGS
                     GAKDRSSVKTCTTCNGAGAVREIRNTFLGQMQTTSTCPTCNGAGKMVTANCTSCKGEG
                     RVNAEETIEFEIPAGVSDGMQLSMAGRGNAGQKNGPAGDLVITVEEQPHEFLERDGNN
                     LLYDLNINIADAAIGTSVEVPTIDGKARIKVPSGTQSGKIFRLRGKGLPALQSYGRGD
                     QLIQVNVWTPQKLNDEEKRLLEKLRSMPNFNPDPEKQPKGFFQRMREMFEG"
     gene            1599..2072
                     /locus_tag="PLG24_05760"
     CDS             1599..2072
                     /locus_tag="PLG24_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HMZ24429.1"
                     /translation="MNKYLVGLLYLLPVILLTSCSGNIYNEERKIAEGIWEYNDSLRF
                     DFEIPDTAQLYTMELDVKHRDEFPYENCYVRIKSLYPDKTLKDDRLSLEFADETGMWM
                     GEKSGGSWLAPIAIQPVAKFRQPGKYSMIFFQDTRQDSLPGIESLRLKIARYKAK"
     gene            complement(2104..2244)
                     /locus_tag="PLG24_05765"
     CDS             complement(2104..2244)
                     /locus_tag="PLG24_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24430.1"
                     /translation="MIRTEGMPVRNNYFALPIIQSKKKKDCCKKFKKGKRCKSCPGRK
                     KD"
     gene            2985..3725
                     /locus_tag="PLG24_05770"
     CDS             2985..3725
                     /locus_tag="PLG24_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HMZ24431.1"
                     /translation="MKVLVCISKTPDTTAKISFKNDGKDFQSDGVQYIMNPYDEWFAL
                     VRGLELTEAQGGTVTVLNVGPADNDTIIRKALAIGANGAVRINKEPEGAWDVAHQIAA
                     FARTQAYDLILLGKETIDYNDSEVGATLAELLDLPFISYANKLDVNGAEAVVNREIEG
                     GTEIMQGQLPLVISCAQGMAEQRIPNMKGIMTAKSKPLNVIDPVAVDSPVTIVRHELP
                     PAKKSVTLIAPEDTDELVRLLHEEAKVI"
     gene            3772..4734
                     /locus_tag="PLG24_05775"
     CDS             3772..4734
                     /locus_tag="PLG24_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HMZ24432.1"
                     /translation="MSVIIFAESVEGQVKKSAFEAVTYGRKVADALGVACKAITLGKA
                     ENASELGVYGAQEVYNAGDAAFDQFDSQVFATAIADAFNQLGGKILVLSHSSMGKSLA
                     GRLSVRLNAGCASGASELPDISNGFIVGKSVYSGKASAFYNINTENKIVSLMGNAIKP
                     EKTGSPVEVKPLAIVAGVPRIKVVERKTISGTVPLPEAALVVSAGRGMKDPSNWGMIE
                     ELAGELGASTACSRPVSDTGWRPHHEHVGQTGLAIRPNLYIAIGISGAIQHLAGVNNS
                     RVIVVINKDPEAPFFKAADYGIVGDLFEVVPRLIESVRKFKAGH"
     gene            4966..5556
                     /locus_tag="PLG24_05780"
     CDS             4966..5556
                     /locus_tag="PLG24_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HMZ24433.1"
                     /translation="MAAKKKIQLDIIALSHSVTQSNNYAVVLGEVKGNRRLPIVIGGY
                     EAQAIAVSMERMVPNRPLTHDLFKNTLDTFNIRIVEIVISDLIEGIFFAQLICEREGD
                     VFEVDSRTSDALAMAVRFDCPIYTFDHIMDSAGVVLEIDAEEESPAEESHHTESTYSS
                     KTTEELNQMLEDSLQEENYEKAALIRDEIKRRKGGS"
     gene            complement(5777..>7960)
                     /locus_tag="PLG24_05785"
     CDS             complement(5777..>7960)
                     /locus_tag="PLG24_05785"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HMZ24434.1"
                     /translation="PRLLGWSFSGVKKSYHEDLNKKIQPYMLKGGIVTENNKSNAINA
                     IKNFFIDKGYLDAMAKVRETSHDTTLNQVKLVFNVTRGKRIKIQDVTFSGNQNVKSRK
                     LRKQLDDTKVKRRLFASSKFIKPKYEEDKTELISYYNTLGFRDAKIISDSMWREKDGD
                     LRIHINLAEGNKYFYRNITFKGNTLYTEEQLKNRLGINKGDEYNQKKLENRLRFSMDG
                     TDVSSLYLDDGYLAFNVDPVETAVSNDSIDLEMRVFEGPQFTIDKVVIKGNDRTNENV
                     IRRELRTLPGAKFSRSDLIRSQREIINLGYFNQENIGINTPVNQKAGTVDIEYTVEEK
                     PNDQLELSAGYGGFQGLIGTLGVSFNNFSIHNIRKKEAWHPLPQGDGQKLSLRAQTNG
                     RFFQSYNASFTEPWFGGKKPTSFTVGGFYTRYSNIDAQYRSIGYYSIASGTMGLGTRL
                     KWPDDNFLVNATLNFQQNKLKDYPGLFTVANGTFNNLNLRLTLARTTVADPIFPRDGS
                     KISLTGQFTPPYSLFNGKSAADYADLPLEKRFHWLEYHKWRLDLEFYKSIIGKLVLKV
                     SNKTGMMSFYNSYVGLSPFERFELGGDGLSNQYVGIVGKDIIALRGYTVEQVREDNKV
                     TTDGAAVFSKISMELRYPISLNPSSSIYVLGFMDGGNMWNRIRDYRPFDLKRSAGLGL
                     RVFLPMFGILGFDYGFGFDKPDLIQNGSKWTQFGKFSFVLGFEPD"
BASE COUNT         2170 a         1550 c         1873 g         2367 t
ORIGIN      
        1 gaaaaagata gttgatgatg tagaagagaa agatcctttt actgaaggtg tacttcttgt
       61 atacaataag ctgttcaatg tgctgaaatc aaaagggctg caggttttgg aatccaatgg
      121 tcagccgttt gatgctgagt tgcatgatgc tatttcgcat attcccgtat cagatccttc
      181 cttgtcaggg aaggtgattg acactgtcga aagaggatat ctgttgaatg ataaattgat
      241 acgccacgcc aaagtagtag tgggggcttg atttgctttt tgattaaaca aactatttga
      301 ttcagactat gtctaaacgc gattattacg aggttcttgg tgtatcgaaa ggagcagatg
      361 aggccgaaat taaaaaagca taccgaaaag ttgcgatgga gttccacccg gatcgtaatc
      421 cgggggataa ggtggctgaa gaaaagttta aggaggcagc tgaggcatat gaaatactga
      481 gcaatccaga taagaaagcc agatatgaca gattgggcca tgcaggcgtg gaccctaatt
      541 ttggtggtgg aggctatggc ggtcatggga tgaccatgga agatattttt aacaactttg
      601 gcagtatttt tgaggactca ccttttggtt cattctttgg tgggggccag cagcgtagac
      661 aaggcggcgg gcaacgtggc agtaatcttc gcatcaagct caaaatgaca ctggaggaaa
      721 tcaataccgg agtcaagaaa aaaatcaggg tcaacaagca ggtgagttgt caaacctgtg
      781 gaggatcagg agccaaagat cgttcttctg tcaagacttg tacgacctgt aatggtgcag
      841 gcgctgtccg cgagataaga aataccttcc tgggacaaat gcagactaca tcaacctgtc
      901 cgacttgtaa tggagcagga aagatggtaa ccgccaattg taccagttgt aagggagaag
      961 gacgagtgaa tgctgaagag accattgaat ttgaaatccc ggcgggtgta tcagatggca
     1021 tgcaactttc tatggccggt cgaggcaatg ccggtcagaa gaatggaccg gccggggacc
     1081 tggttatcac cgttgaggaa cagccccatg agttcctaga aagagatggc aataatctgt
     1141 tgtacgactt gaatatcaat attgcggatg cggcaatcgg cacttcggtt gaagttccta
     1201 ccatcgacgg taaagcgcgg atcaaggtgc cttccggaac acagtccggt aaaatatttc
     1261 gtttgagggg taagggcctt cctgccttac aaagctacgg gcgtggtgat cagctgattc
     1321 aggtgaatgt ctggacaccg cagaagctca atgatgaaga gaagcgtttg ctggagaaat
     1381 tgcggtccat gcctaacttc aatcctgatc cggaaaaaca acccaaaggt tttttccagc
     1441 ggatgaggga aatgtttgaa ggttgatgtg ccggtgtggg gttgaagaaa tgaggctttc
     1501 ttaaattttt tttgcctcta cacgctcagt ctgaatattg taaatatttt cgtattgtaa
     1561 acatgcacta atattgccgt attaaaactg cttgccagat gaataaatac ttagtcggac
     1621 tgttatatct tctgcctgtc atcctgttga catcgtgctc cgggaatatc tataatgagg
     1681 agagaaagat tgcggaaggc atttgggaat acaatgattc actaagattt gactttgaaa
     1741 ttcctgacac ggcacagctg tacacgatgg agctggatgt gaagcacagg gatgaatttc
     1801 cttatgaaaa ttgctatgta agaatcaagt cactgtatcc ggacaagacc ttgaaggacg
     1861 atagactttc actggagttt gctgacgaga caggtatgtg gatgggcgag aagagcggcg
     1921 gtagttggct tgctccgata gctattcaac ctgttgccaa gttccggcag ccgggaaagt
     1981 attctatgat attctttcag gatacaaggc aggatagcct tccggggatc gagtctttaa
     2041 ggcttaagat tgccaggtac aaagcgaaat gatattttaa aaattaagag tcaaaggatt
     2101 cgatcaatcc ttttttcttc ccgggcagga cttacaacgt tttccttttt taaatttctt
     2161 gcaacaatcc tttttctttt tactctggat gatgggcaat gcaaagtagt tgtttctcac
     2221 aggcattccc tccgttcgaa tcatattttg ttgcattctt tttttgttgc aaatgtatat
     2281 ttatttagat taaatacaaa taatatttga tgctaaatta aaattaatac ctgatcccat
     2341 gaatacttat ttattgtgga ttttcaatgc tttacaatat atcgccgttg atagatttgg
     2401 aacaggattc cggttcattt tgattggtaa aagccatcgc ttggaaagtt gttcttgggc
     2461 ttaatactta tgggtacaca ataaattttt aattctcctt gtggaacaag accgcagttt
     2521 aagcaggcag ctaagtgtct gtttgaaatg gggaatagtc gcaggttttg ccttactgtc
     2581 tgatttatga agtcgttttt ccaaaactgt gccttcagta tcccattgct tttaaaataa
     2641 atgattccac cttgtattca tttatcgcga gccttttgat cgtctttgga agcctcatca
     2701 ggggagtctg atttctagtt tgcctgccct gtggtgtaag tagtcaatga ggatattcaa
     2761 cgcttcaatt tgttcagtgt tcatcagtat tttaggattt gatgctttgt ttgaatgttt
     2821 tttctttgag tatcaataat tttacttcaa tctcatagga ttttcgtata tttgcatctt
     2881 gtgtacaaaa taatgttttg taaaatcttt aattattatt gatttgtaaa atagaatttt
     2941 gttttcagat ttatttgcct ttaaacttaa aatttattga atcaatgaag gttttagttt
     3001 gtatcagtaa aacacccgac actactgcca aaatctcatt taagaacgat gggaaggact
     3061 ttcagtctga tggtgttcaa tatatcatga atccttacga tgaatggttt gccctggtcc
     3121 gtggcttgga attgactgaa gctcaaggtg gaacggtaac ggtacttaac gtcggacctg
     3181 ccgacaatga caccatcatc cgaaaagctt tggcgatagg agccaatggt gcagtgagga
     3241 tcaataagga gccggaaggt gcatgggatg tggctcatca gatcgccgca tttgcccgga
     3301 ctcaggctta tgatcttatc cttttgggta aggagacgat tgactataac gacagtgaag
     3361 ttggagccac tttggcagaa ctgcttgacc tgccgtttat ctcatatgcc aataagctgg
     3421 atgtgaatgg tgccgaggcc gttgtcaacc gtgaaattga aggaggaaca gaaatcatgc
     3481 agggacagct tccccttgtc atcagctgtg cacaaggcat ggctgagcaa aggataccga
     3541 acatgaaggg aattatgact gccaagagca agccgcttaa cgtaatcgat cctgtggctg
     3601 ttgattcgcc ggtgacaatt gtgcgacatg aattgcctcc ggccaagaaa tcagtgacgc
     3661 tgattgctcc ggaagatacg gacgagttgg tgcgcctgtt gcatgaagag gcgaaagtca
     3721 tttgatattg ggtcaatcta atttttcaaa cttttaatta ttaaaatata tatgtcagtt
     3781 atcatatttg ccgaatcggt tgaaggtcag gtcaagaaaa gcgcatttga agcagtaact
     3841 tatggccgca aggtggcgga tgcccttgga gttgcttgta aggctataac attgggaaag
     3901 gcagaaaatg cttcagagct gggtgtttat ggggcacaag aggtttataa tgcaggagat
     3961 gcagcatttg atcagtttga ttctcaggtt tttgctactg ccattgccga tgctttcaat
     4021 caattgggcg gcaagatttt ggtcctttca catagctcca tgggcaagtc cctggcaggc
     4081 cgtctttcag taagactcaa tgctggctgt gcttcaggtg ccagtgaatt gccggatata
     4141 tccaatggct tcattgttgg taaaagtgta tattccggta aagcttctgc attttacaat
     4201 attaatacag agaataagat tgtttccctc atgggaaatg ccattaagcc ggagaaaacc
     4261 ggatctcctg tcgaagtgaa gccactcgca atcgtggcag gggtacctag aattaaggtt
     4321 gttgaacgaa agaccattag cggtactgtt ccactgccgg aagctgcttt agtggtgtcc
     4381 gcaggacgag gaatgaagga tccatccaac tggggcatga tcgaagaact ggccggagaa
     4441 cttggagcct ctacggcatg ttcaagaccc gtttctgata ccggctggag gccgcatcat
     4501 gagcatgtag gacagaccgg attggctatt cgtcctaatc tttatatagc catcggtatt
     4561 tccggcgcaa tccagcacct ggccggtgtc aacaattctc gcgtaatcgt cgtcatcaat
     4621 aaggaccctg aagcaccttt cttcaaggct gctgattatg gtattgttgg agatttgttt
     4681 gaggttgtac caaggttgat cgaatctgtc aggaagttca aagccgggca ttgatttttc
     4741 aaagttgccg gaaaaaacag ctgcacggcg gtggttgaaa atattcagat attttaaccc
     4801 cgggttaatt gaccattaat tcaaggtggt tttttggaat aaatactttt cattggggca
     4861 tagcgcatgt tgttttttcc ggttattatc tttttaccga ttccggcagt aactacataa
     4921 cgtataaata cgtatttttg agcatcataa ctaaacaaag ccctcttggc agcaaagaag
     4981 aaaatccagc ttgatatcat agcgttatcg cacagcgtga cccagtccaa caattatgca
     5041 gttgtgcttg gtgaggtgaa aggcaacagg cggttgccca tcgtcatcgg tggctacgaa
     5101 gctcaggcca tagcagtatc tatggaacgt atggttccca ataggccgtt gacccacgat
     5161 ttgttcaaga acacgctgga tacatttaat attcggattg ttgagatcgt catcagtgat
     5221 ttgattgaag gcattttctt tgctcagctg atttgtgagc gggaaggaga tgtgtttgag
     5281 gtggactcca gaacatctga tgccctggcc atggctgtga gatttgattg tcccatctac
     5341 acctttgatc atataatgga cagtgccgga gtggtgctgg aaatcgatgc tgaagaagaa
     5401 agccctgctg aagaaagcca ccatacagaa agcacttatt catccaaaac aacagaagaa
     5461 ttgaatcaga tgctcgagga ttccttgcag gaagagaatt atgaaaaggc agctctgatt
     5521 cgtgatgaaa taaaaagacg taaaggcggt agttgacacc attacacgct tgctttttgg
     5581 aatgttgtaa ttgcggatgt tgagcactaa agagggaata ttcctatacc aaatcaagca
     5641 ccacgatttt ccggatttta tctttctatc ctgatattga caatttgctc aagtttcatt
     5701 atcacatgat actgaacata cgaagggtgt aaccgattgg ctacctttct cgaaaagggt
     5761 atattaatat tgtagcttaa tccggttcga atccaagtac gaagctgaac tttccgaact
     5821 gtgtccattt ggaaccattt tggatcaagt ccggcttgtc gaacccgaat ccatagtcaa
     5881 atcctaagat tccaaacatg ggcaggaata ccctaaggcc caatcctgca gagcgcttca
     5941 agtcaaatgg tctgtaatcc ctgatcctgt tccacatatt tcctccatcc ataaatccaa
     6001 gtacataaat agaggaagat ggattaagcg atatcggata tctcaattcc atagagatct
     6061 tggagaatac tgcagcccca tcggtggtaa ccttgttgtc ttcccttact tgttctacgg
     6121 tatatcctcg cagggctatg atatcctttc ccacgatgcc cacatattgg ttggatagtc
     6181 catcaccacc caattcaaac ctctcgaatg gcgataggcc tacataggaa ttatagaagc
     6241 tcatcattcc ggttttattg gaaactttca agacaagttt acctatgatg gatttataaa
     6301 attccagatc gagacgccat ttatgatatt caagccagtg gaatcgcttt tccaggggca
     6361 ggtctgcgta gtctgctgca gatttgccat tgaataatga gtatggaggc gtgaattgcc
     6421 cggtaagaga tattttagaa ccatcccgtg ggaagatagg gtccgcaacg gttgtccttg
     6481 caagggtgag ccgtagattc aggttgttga aggttccatt agccacagtg aaaagcccgg
     6541 gataatcttt taatttgttt tgttggaaat tgagtgttgc attgaccagg aagttgtcat
     6601 caggccactt aagtcgagtg ccgagaccca ttgttccgga tgctatgctg taatatccta
     6661 tacttctata ttgcgcatca atattggaat aacgggtata aaatccacca acggtgaagg
     6721 atgttggttt tttcccacca aaccatggtt cggtaaatga tgcgttgtac gattggaaaa
     6781 aacgaccatt ggtttgagct ctgagagata atttctgtcc atcgccctgt ggtagtggat
     6841 gccaggcttc tttctttctg atattatgga tcgaaaaatt attgaagctg actccaagag
     6901 tgcctataag tccttggaag cctccgtatc cggcggataa ttccagctga tcattgggct
     6961 tttcttccac ggtgtattca atatctacgg ttccggcttt ttggttgacc ggcgtattga
     7021 taccgatatt ttcctgattg aagtatccaa gattaatgat ttcacgctga gaacgaatca
     7081 ggtcgcttcg gctgaatttg gcgcccggaa gtgttctaag ctctcgccga atcacattct
     7141 cattggttcg gtcgttgccc ttgattacta ccttatcaat agtaaattga ggtccttcaa
     7201 acacccgcat ctcaaggtca atagaatcgt tggagacagc cgtttcaacc ggatctacat
     7261 tgaaggcgag atatccatcg tccaagtaca aggaactgac gtctgtcccg tccatactga
     7321 atcgtagcct gttttccagc tttttctgat tatattcatc gcccttattg attcccaggc
     7381 ggtttttcaa ttgctcttcg gtgtatagtg tatttccttt gaaagtaatg tttctgtaaa
     7441 aatatttatt accttcagcc agattgatgt ggatacgcag gtcgccatcc ttctctctcc
     7501 acatagaatc tgaaattatc ttcgcatccc gaaatccgag tgtgttgtaa taggaaatca
     7561 actctgtttt gtcttcttca tattttggtt tgataaattt agaagaggca aacagacggc
     7621 gcttgacctt tgtatcatca agctgtttgc gaagtttacg tgattttaca ttttgattgc
     7681 cactgaaggt tacatcctga atcttgatgc gtttgcccct ggttacattg aatactaatt
     7741 tgacctggtt gagcgtcgtg tcgtgacttg tctctcttac tttagccatt gcatcaagat
     7801 aacctttgtc gataaagaaa ttcttaatgg cattgatggc gttgctttta ttattttctg
     7861 taacgatgcc tcctttcaac atataaggct gaatcttttt attcagatct tcatgatagg
     7921 atttcttaac accggaaaaa gaccatccca acagcctggg
//
LOCUS       DAOQQN010000116         7887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3627_length_7887_cov_4.863892, whole genome shotgun sequence.
ACCESSION   DAOQQN010000116 DAOQQN010000000
VERSION     DAOQQN010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7887
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3627_length_7887_cov_4.863892"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..545
                     /locus_tag="PLG24_05790"
     CDS             141..545
                     /locus_tag="PLG24_05790"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24435.1"
                     /translation="MKFFSKSNLFKVSTLILLSGMWMTSCYYDKESDLPKDPDNNPTD
                     TCDLSQAKYSLKIQKILADNCVVCHGHGLQEGNVRLDSHEKVTIQVNNGKLLKSISHQ
                     AGASPMPYGLPKMNDCNIAMIEQWIKNGALND"
     gene            620..1030
                     /locus_tag="PLG24_05795"
     CDS             620..1030
                     /locus_tag="PLG24_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24436.1"
                     /translation="MKKVLALILVGLVVAAGVWFYVNKGHEDVAGEKPELSINATELV
                     KAFETNEQEANKQYVGKLIEVSGKIVQIDLTEDGSVNLLLESDNPMSTVLCQLDPLQK
                     QDQSGISEGREVRIKGLCSGFTTDVVLDRCHIIR"
     gene            1097..1666
                     /locus_tag="PLG24_05800"
     CDS             1097..1666
                     /locus_tag="PLG24_05800"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMZ24437.1"
                     /translation="MYDTSFFRFTFVTLVLTFLSLGAMNAQGKYFTRSGTISFVSDAL
                     LEKNEAVNNKVSAAIDASTGKVEFIVLIKSFQFKKALMQEHFNENYMESGKYPKSTFS
                     GRITNLKDIDFTKDGAYKVMVTGVLTMHGVTKDVATQGTINIVKGVANVQSKIRVKLA
                     DYNIQIPAIVKDKVAQEVDVMIDCKLKKV"
     gene            1730..2632
                     /locus_tag="PLG24_05805"
     CDS             1730..2632
                     /locus_tag="PLG24_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HMZ24438.1"
                     /translation="MRNKLYASLLVVALLISVKSYSQSDSSLIAMLDGSAQDVDYTYA
                     TFKTNRIINLHSIENTAAGVLDVKISHRFGLINQGLYDIFGLDAAQMHLNFDLGLTDR
                     LAIGVGRTSLDKAYNGFVKYKILKQSTGSKNMPVSVSFAAGTVIKTSRSLDPDPGNTF
                     WYRVYYDFGLLIARKFSENFSLQVTPTLVHRNLVRTAEEKNDVFAIGVGARQKLTRRM
                     SINAEYIYVLPGQLAPDKLNSLSVGLDIETGGHVFQLHFTNSQGMNENAFITETINRW
                     DKFEFRFGFNLSRVFTLWEPAIRD"
     gene            complement(2806..3258)
                     /locus_tag="PLG24_05810"
     CDS             complement(2806..3258)
                     /locus_tag="PLG24_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMZ24439.1"
                     /translation="MDDYLQFAPIVAAYGMDLLGVPSKNDFINRSVILLKGELAMAGI
                     TTLIKYTTQYNRPDHSNRHSFPSGHTAQAFVAATFLSEEYKDRLPWIPYISYGVASSV
                     GILRMANNKHYLSDVLAGAGIGILTMKVAYWTHKYKWNNKKKEKYSEF"
     gene            complement(3283..3519)
                     /locus_tag="PLG24_05815"
     CDS             complement(3283..3519)
                     /locus_tag="PLG24_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24440.1"
                     /translation="MFSTFLFTMFLFLSGFCLCQQKTIEPDCGLKVSYYNLSHRVAFQ
                     PRKLIVPAGLIVTGTALNFKPIKSVKSNIVSVRN"
     gene            complement(3577..4104)
                     /locus_tag="PLG24_05820"
     CDS             complement(3577..4104)
                     /locus_tag="PLG24_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24441.1"
                     /translation="MDRAELDAQSVFYEKQSFRQWWIWLILLGVDGIFVVGAILQLFM
                     GRQFGDNPMPDFVLILVCGVILFSSFLFYSIHLETRICWDGIYVRFFPFHFKYKIFYW
                     DTIKKCYVREYAPVKEYGGWGIRNSLNGRKKAYNISGNQGIQLEFTDGKNLLIGTQKV
                     DEVINTLKYLKRWKA"
     gene            complement(4263..5309)
                     /locus_tag="PLG24_05825"
     CDS             complement(4263..5309)
                     /locus_tag="PLG24_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ24442.1"
                     /translation="MNTKHVLVTGASGTVGREVVKILADQSDFRITVFDIANQQSEPF
                     FNSFGERIQFIPGNISDVADVSRIPEDLDAVIHLAAIIPPLADEDPGLARKVNVEGTG
                     LLIDHIERTSPGAFFLYSSSVSVYGDRVENPDIRVTDDLKISEGDEYGQTKLEAESII
                     QSSNLSWSVFRLAAIMGNHKISRLMFHMPLETSLEICTPEDTARAFVHALSAQDRLEY
                     RIFNLGGGKQCRSSYREFLERSFNIFGLGRLDFPKQAFALHNYHCGNMADGDELEEIL
                     HFRRDTMDSYFRKTANSVSPLKRWFTMLFRPIIKWFLLRQSEPYRAYRKGDVRMMSQF
                     FPLLPEFNLKAVER"
     gene            complement(5409..6536)
                     /locus_tag="PLG24_05830"
     CDS             complement(5409..6536)
                     /locus_tag="PLG24_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ24443.1"
                     /translation="MRSSSILYICIYNRLYMLTLAWIGVSQGFFAAILMVAKQKNSVS
                     DKILSAWLFLFGLDFLSCALDYMIFGKPLLTSSFLLFNPALYLYVKSLVLPRFRLRWT
                     QLLHLLPYIIFKVLAYVYREPFTMEGYFERDGHLVYRLIFGASTIVSWLVYNSLTLKY
                     VHLYRVKLFHEQSNIEKSNNLTWVLTVAVFYVSYCIIAFMMAVAAYIGWFGPLSPHIY
                     NYFVLLFAIYILSFYGLYQQELPEHLLIEESVPVAYKNSSLSPEAKEDIRKRIEKFMI
                     GQKPYLNPGLNMDMLSEALNIPKYQLTEVLNTVIGSNFFQYVNKFRVEAVKDMLLDPT
                     NHFSIEAIGFECGFASKSSFYSVFKNMTGKTPIAFRESVVS"
     gene            complement(6677..7096)
                     /locus_tag="PLG24_05835"
     CDS             complement(6677..7096)
                     /locus_tag="PLG24_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMZ24444.1"
                     /translation="MNTNKLVSEVMTSNVMTADRRDSLRHVNSLMKEKHIRHLPVVDN
                     GKLVGIVSNTDIMRLSFGDLYEGETGADEAMFDMLTLDQVMVHRPVSVRHDEFVEDVA
                     LKLASAEYHAFPVIDGDKVVGIITTTDVIKYLLNRGE"
BASE COUNT         2520 a         1599 c         1559 g         2209 t
ORIGIN      
        1 aaaaggcctt gccgttcaaa aaattttatc cgaagtttca aattctccca aaaaattcgg
       61 tccgggaagt aactttttta atttgactta ctctaatagt gaataagata attgacaaaa
      121 atttttaaat tttttttcaa atgaaatttt tttcaaaaag caatctattc aaggtaagta
      181 ctttaatatt gttgagtggc atgtggatga cttcatgtta ttatgataaa gaatccgatt
      241 tgccaaaaga tccggacaat aatccgaccg acacgtgtga tttatcacaa gcaaaatata
      301 gtcttaaaat acagaaaata cttgcagaca attgtgtagt atgccatggt catggtctcc
      361 aagagggaaa tgtcagactt gattcccatg aaaaggttac gattcaagtc aataacggca
      421 aattacttaa gtcaatcagt catcaagccg gcgcatctcc aatgccttat ggtctgccca
      481 aaatgaacga ttgcaatatt gcaatgattg agcaatggat aaaaaacggt gcattaaatg
      541 attagattga ctattttgct gattatatag gccttgaaaa acaattcgtt ctttttataa
      601 aaagtaaaaa tttggtaaaa tgaagaaggt actggcttta atattggtag gccttgttgt
      661 agcagcagga gtttggtttt atgtcaataa aggtcatgag gatgtggctg gagaaaaacc
      721 cgaactgtcc atcaacgcta cagaactggt caaggcattc gaaactaatg aacaagaagc
      781 caacaagcaa tatgtaggta agctaattga agtaagcggg aaaattgtac aaatagacct
      841 tacagaggac ggctctgtaa atctgttact cgagtcggat aatcccatga gtaccgtact
      901 ttgtcagctg gatccattgc aaaaacaaga ccaatcaggc atttcggaag gaagagaggt
      961 ccggatcaaa ggtctatgct cgggttttac taccgatgtt gtacttgaca gatgccatat
     1021 tatcaggtaa atttggatga caattagatt atatttacac aaaatgcatt aaatcatatt
     1081 ccaacaaaga ttgattatgt acgatacctc cttttttaga ttcacatttg taaccttagt
     1141 attaacattt ctttcattgg gagcaatgaa tgctcaaggt aaatatttta ctcgctcagg
     1201 gacgatcagt tttgtctcag atgccttgct ggaaaaaaat gaagcggtca acaacaaagt
     1261 aagtgcagcc attgacgcct caaccggaaa ggtggaattc atcgtattga tcaagtcctt
     1321 ccagtttaaa aaagcattga tgcaggagca tttcaacgaa aattacatgg aatccggcaa
     1381 atatcccaaa tccacatttt ccggccggat caccaacctt aaagatatcg acttcacaaa
     1441 agatggagct tataaggtga tggttacagg tgttctgacc atgcatggag taaccaagga
     1501 tgtagccacc cagggtacaa tcaatatcgt caaaggagtt gccaatgtgc aaagtaagat
     1561 tcgggtgaag ctggctgatt acaacataca gattccggct atcgtcaagg ataaggtggc
     1621 acaggaagtg gatgtaatga tcgattgtaa gttgaaaaaa gtttaatgac ctgcgaagct
     1681 taggattctg cgcctgattt caatattttt tcatcattaa aaatctctaa tgcggaacaa
     1741 actatacgct agtctattgg ttgtcgctct cctcatttca gtcaaatctt atagccaatc
     1801 cgacagcagt cttattgcaa tgctcgatgg ttcggcacag gatgtcgatt atacgtatgc
     1861 tacctttaag accaaccgaa ttatcaacct tcattccata gaaaacacag ctgccggtgt
     1921 gttggatgtg aagataagcc atcggtttgg gctcatcaac caggggcttt atgatatatt
     1981 tggattggat gccgctcaga tgcacttgaa ttttgatctt ggcctgacag acaggctggc
     2041 aatcggtgtt ggtcgtacat ccctggacaa ggcttataat gggtttgtga aatacaaaat
     2101 attaaagcaa agcacgggca gtaaaaatat gcctgtttcc gtttcatttg ctgcaggaac
     2161 agtaatcaaa acctcacgat cgcttgatcc tgatcccggc aacacattct ggtacagggt
     2221 ttattatgat tttgggctgt taattgcgag gaaattttca gagaatttta gtttgcaggt
     2281 aacaccgacc cttgtccatc ggaatctcgt acgtaccgca gaagaaaaga atgatgtatt
     2341 tgccataggc gtgggtgcca gacaaaaact gacgcggagg atgagtatca acgcagaata
     2401 tatctatgtt ttaccgggtc agcttgctcc tgacaagtta aattcgcttt ctgtagggct
     2461 tgatatcgag accggcgggc atgtatttca gctgcatttt accaacagcc aagggatgaa
     2521 tgaaaatgca tttatcacag agaccataaa caggtgggac aagtttgaat tccggttcgg
     2581 cttcaacctt tcacgcgttt tcacgctttg ggagcccgcg attcgggatt gattaccggg
     2641 aaatactact catgaaatta tctgaggatc atcatcgtcc cggtgatgtt gtgagttccg
     2701 cttgtggcac ttgctaaggt cagcatcttt actaaacaga tcacattttg cccaagcccc
     2761 tcgttcattt ttgaaataga taactttcct tcagggacaa tcctgtcaaa actccgaata
     2821 tttttctttc ttcttgttat tccacttata tttatgtgtc cagtaggcaa ctttcattgt
     2881 caggatgcca ataccggccc cggcaagtac atcactgaga taatgcttgt tgttggccat
     2941 tcggaggatg ccaactgaag atgctacacc gtagctgata tacggaatcc atggcaggcg
     3001 gtccttatac tcttcactaa ggaaggtggc agcgacaaat gcttgagcag tatgtcccga
     3061 aggaaatgaa tgtctgttgg agtggtcggg gcggttatat tgggttgtat atttgatcag
     3121 tgttgtgata ccggccattg ccagttcgcc tttcaaaaga ataacgcttc ggttgataaa
     3181 gtcattcttt gatggcactc ccaaaaggtc catgccatat gctgccacaa taggagcaaa
     3241 ctgaagatag tcgtccacat aaatgcgaaa acccggcaga tattagttgc gtacacttac
     3301 gatattgctc ttaacgcttt tgataggctt aaaattgagg gcagtcccgg taacgatcaa
     3361 accagcaggg acaatcaatt tacgcggctg aaaggcaacc cggtgagata ggttataata
     3421 ggatacctta agaccacaat ccggctcaat cgttttttgc tgacagaggc agaaaccgga
     3481 aagaaaaaga aacatagtaa acagaaaagt cgagaacatt ccggaatatt ttaggcaaaa
     3541 atatactttt tttaaaagac cagtcatagc gccaatttat gctttccaac gcttaagata
     3601 tttgagcgta tttatgactt catcaacctt ctgagttcct atgagcaaat ttttaccatc
     3661 tgtaaactcc agttgaattc cttgattacc ggagatgttg taggcttttt tccgtccgtt
     3721 caaactgttt cggatccccc atcctccata ttctttgaca ggcgcatatt ctcgaacata
     3781 acacttcttg atggtatccc aatagaaaat cttgtattta aaatggaatg gaaaaaatct
     3841 cacatagatt ccatcccagc aaatcctcgt ctccaaatgg atggaataaa aaagaaaact
     3901 cgaaaataat ataacgccac acacaagaat caatacaaaa tcaggcatag ggttgtcacc
     3961 aaactgcctg cccatgaata attggagaat cgcaccaacg acaaaaatac catcgacacc
     4021 cagaagaatc aaccaaatcc accattgccg gaaactttgt ttttcgtaaa aaacagattg
     4081 tgcatccaat tccgctctat ccattatcaa ataatttaat ttcaaattta ctgcaaaaaa
     4141 cgcaataaaa cgccctcaat caaagaataa atagtataaa aaatccggat tccattaaat
     4201 taatgaaatc cggagaataa acttaaaaaa actaaaaaac aaaatcctgt tcgggtgatg
     4261 atttaccttt caacagcctt tagattaaat tccggcagca aaggaaagaa ctgggacatc
     4321 atcctgacat caccctttcg ataagcccga taaggctctg actgacgaag aaggaaccat
     4381 ttaatgatcg gccggaatag catggtaaac catcttttca agggagaaac tgaatttgct
     4441 gtcttccgga aataagaatc cattgtatcc cgtcgaaaat gaaggatttc ttccagctcg
     4501 tcaccatctg ccatattgcc acaatgatag ttgtgaagcg caaaagcctg ttttggaaaa
     4561 tccagtcgac ccaatccgaa aatattgaag gaacgctcca aaaattcacg ataagacgat
     4621 ctgcattgtt ttccgcctcc gagattgaaa atccggtatt ctaatcgatc ctgtgcggat
     4681 aaggcatgta cgaatgctct ggccgtatct tcaggtgtac atatctccag gcttgtttcc
     4741 agaggcatat gaaacatcag tcgagaaatc ttatgattgc ccataatagc tgccaatcga
     4801 aatactgacc aactaagatt acttgattgg ataatagatt ccgcttccag tttggtttga
     4861 ccatattcat ccccttccga tatcttcagg tcatccgtaa ctctgatatc cggattttca
     4921 acacggtcac catataccga aacagatgaa ctatatagaa agaaagctcc gggtgatgtt
     4981 ctttctatat gatcaatcaa caaaccggtc ccttcgacat tgacttttcg tgcaagtccg
     5041 ggatcttcat ctgccaatgg tggtatgatg gcagccagat gtatgacagc atccagatct
     5101 tccggaattc ttgacacatc tgccacatcg cttatattgc cggggatgaa ctgaatgcgt
     5161 tcgccgaatg agttgaaaaa aggctcagat tgttggttcg ctatatcaaa gactgtaatc
     5221 cgaaaatcac tttggtccgc caatatcttt acaacctctc ttccaaccgt acctgatgca
     5281 cctgtgacta aaacatgttt tgtattcatt tgaaatcatt tttgcaatga taacggagaa
     5341 aattgagcaa aatagtccag gttttaaaga ttggactaca tgtttatgtc ctgattgcaa
     5401 aattcagact aggaaaccac agattcccta aaggcaatcg gagttttgcc ggtcatgttt
     5461 ttaaaaacgg aataaaaaga ggatttactg gcaaatccgc attcaaagcc gatcgcttca
     5521 atggaaaaat gattggtcgg atctaaaagc atatccttca cggcttcgac cctgaatttg
     5581 ttgacatatt gaaaaaaatt gctcccaata actgtattca atacttcagt caattggtat
     5641 ttaggtatat tcaacgcttc agaaagcata tccatattca gcccgggatt aagatatggc
     5701 ttttgcccga tcataaattt ttcaatgcgc ttcctgatat cttccttggc ttcagggctt
     5761 aacgaagagt ttttataagc cacgggcacc gactcctcta tgaggagatg ctccggcaac
     5821 tcctgctggt aaagaccgta aaaactcagg atgtagattg caaataatag aacaaaatag
     5881 ttgtaaatat gtgggcttag cggtccaaac cagcctatat aggcagcaac agccatcata
     5941 aaggcaatga tgcaataact gacataaaat acagcaaccg tcaaaaccca ggttaggttg
     6001 ttgctttttt caatgttgga ttgttcatgg aataatttca cccgatacag atgtacatac
     6061 ttgagtgtaa gggaattata taccaaccag gaaacgatag ttgacgcacc gaatataagc
     6121 ctgtaaacca aatggccatc ccgttcaaaa tagccttcca tcgtaaaggg ttcccggtaa
     6181 acatacgcca acaccttaaa gatgatgtag ggcaaaagat ggagtaattg ggtccatcta
     6241 agtctgaacc gaggcaatac caaagatttg acgtacagat acaaagccgg attgaacagg
     6301 agaaaggagc ttgtcagtaa aggcttacca aaaatcatat aatccagagc acaacttaga
     6361 aagtccagtc cgaaaagaaa taaccaggcg gataaaatct tatccgatac ggaattcttc
     6421 tgtttagcca ccatgaggat agccgcaaaa aaaccctgac taacaccaat ccaggcaagt
     6481 gtcaacatgt acagtctatt atagatacaa atatacagga tggaagatga acgcatagga
     6541 tattatgcgt tatcatcata gattagcaga tgatgtcaac atattgctgc aaatcagaat
     6601 caaggattcc gacttcaaca aatgaagatt atggatccaa ccttcctgtt attgaagcaa
     6661 tcccttccat tcccaactat tcacccctgt tgagcaggta tttgataaca tccgtcgtgg
     6721 tgataatgcc caccaccttg tccccatcta taaccggaaa agcatgatat tcagcagaag
     6781 caagcttgag agccacatct tcgacaaatt catcatgacg aacactgacc ggcctatgaa
     6841 ccatcacctg atcaagagtg agcatatcga acatggcctc atccgccccg gtctctcctt
     6901 catacagatc accgaaactc aggcgcatga tgtcggtatt gctgacgatg cctaccaatt
     6961 ttccattgtc aactaccggc aaatggcgta tatgtttctc cttcatgagt gaatttacat
     7021 gacgcaaact atctctcctg tcagcagtca ttacgttgct ggtcattact tcggatacca
     7081 atttattcgt attcatttca aaattattat gatgagtaaa tttcgtgaag taatcaaata
     7141 caatttatga ttctgcgcat agttttctaa aaaaatgaag tcttttgaaa aaacctgaat
     7201 gcgtgtacat taatcggcaa atcaaattat ataactgact ttgataagag atgattcatt
     7261 ggggaatccc ggtcagaatg tgtttgggct aagctgtata attctttaag ctcccgggat
     7321 tgtaaagaga cttgatcaac caaaaagtgt ggctcattga tggccctgag cctgcctgat
     7381 agctcagatt cagttagatg tgcggaagta tcgctggttg accttgggta tgtattgaaa
     7441 ttaaatacat gtcaaggtca aataagaaaa tattacggtg ggaccttaac caatatttga
     7501 tcaaactacc gacaataatg tgtaaaaaat tatataatat gtataattat gtcaatctga
     7561 ttattacaaa ttaaatattt ttagcaaata ataaatcgac ggataaaaac acaatccgca
     7621 tgttgaaaac tggtaaaggg aaatcatttt acactgttta caaggtattg attattgcaa
     7681 gaaattgccg gccaatagaa ggatcggtgc ccaatagagg taaataataa cgatatttca
     7741 aataactttg cctgccgaat tgaattatgt caggatttcc taaatttttc caccatttta
     7801 atcagtcgac tgaaggtatt tcaatgccgg atcgatttac ctttcctttt tattacgagc
     7861 cgcacccatt ggccgtattg gctgccg
//
LOCUS       DAOQQN010000117         7884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3629_length_7884_cov_5.583983, whole genome shotgun sequence.
ACCESSION   DAOQQN010000117 DAOQQN010000000
VERSION     DAOQQN010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7884
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3629_length_7884_cov_5.583983"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PLG24_05840"
     CDS             complement(<1..375)
                     /locus_tag="PLG24_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HMZ24445.1"
                     /translation="MESLLYLLPLFGVVGLLYMAYQFSWVNKQDEGEGKMKIIAGHIR
                     EGAMAFLKAEYRVLAVFVVIAGIALGILSTIANTSHWFIVIAFVIGAFSSALAGFIGM
                     RVATAANVRTTQAARTSLSKALK"
     gene            515..991
                     /locus_tag="PLG24_05845"
     CDS             515..991
                     /locus_tag="PLG24_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HMZ24446.1"
                     /translation="MTYYVESMKNHVSFLVRLLCLIWVLVGCKGKPEQAYSGVLKPAS
                     DTQTEYLSANKNGTQEKNIHIPEYVYEILVYIRTHNNAPKGYVGGRTFHNRERHLPET
                     EADGSRIRYREWDVHPKVRGRNRGPERLITSKRSAYYTKDHYKTFIYINETFFSKK"
     gene            988..1422
                     /locus_tag="PLG24_05850"
     CDS             988..1422
                     /locus_tag="PLG24_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HMZ24447.1"
                     /translation="MKYIRIASPDTEIKRGNNKITIEVNGSEVRDIKSFYEILVNNLE
                     FPGYFGDNLDALYDMLIDLQWLKQDTIDIIISDYEDFLTDEVDEDKAEIMLLLEDVCR
                     EWKEGYSDDEDWEMKKVRVYVLCDEMTGKQISRMIADMTEEE"
     gene            complement(1430..2389)
                     /locus_tag="PLG24_05855"
     CDS             complement(1430..2389)
                     /locus_tag="PLG24_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24448.1"
                     /translation="MILKSISNFFSNKVVENILLFLILSGVIATIITPDLPFVGKSSD
                     FANQIMIFYLALSMFFLLISQQRLMIAALICTGIFAFFLKETTNARIRYALPNQEEKL
                     RLALINLSSIDSDPRKVFQYVSKKNHDILIFQEYTPEWQGAIENQVEPNFSYKISLPR
                     IDVYGLKVLSQYPIVTADTIFYNDIPLLKIQLLKNNKRFDVFTAYFPPQLDANSREIA
                     LGELDIINSRIEAMQVPLIVTGVFNMVPWSAEIQDFKQKGGFTESRSGYIPSMDKTSG
                     GIFGKPVTHIFYNQLLECTGFSVSRADNDEYGVEAQFQIKNIH"
     gene            complement(2392..2811)
                     /locus_tag="PLG24_05860"
     CDS             complement(2392..2811)
                     /locus_tag="PLG24_05860"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HMZ24449.1"
                     /translation="MATNRTFTMIKPDAVQDGHIGAILAQINAAGFRIVAMKLTQLSP
                     AKAGKFYEVHKERPFYGELVSFMSSGPIVAAILEKDNAVEDFRKLIGATDPSKAEPGT
                     IRALYARNVGENAVHGSDSDENAAIESAFHFAGTEEF"
     gene            2981..3403
                     /locus_tag="PLG24_05865"
     CDS             2981..3403
                     /locus_tag="PLG24_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24450.1"
                     /translation="MIKLLLKIAIPVIFCLLVYNYFFGTPEEKNTSKAVFSKTKDLTV
                     SIYDLLKSEKHKFDQGKYDKALDKLNEIYDAARSKSDGMSEADKAELKKLESEKQDLK
                     REIDQTKKLDAKSADEKSKSLDEKLMELVDKTNRLLGE"
     gene            3521..3602
                     /locus_tag="PLG24_05870"
     tRNA            3521..3602
                     /locus_tag="PLG24_05870"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3555..3557,aa:Leu,seq:tag)
     gene            complement(3794..4222)
                     /locus_tag="PLG24_05875"
     CDS             complement(3794..4222)
                     /locus_tag="PLG24_05875"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HMZ24451.1"
                     /translation="MKNFIIIFASMLISFSGCQQDKKPGDTTEGTKTETVDNKNTEGV
                     FMDFTVKDVGAIPRDIPITVQVVITNNKPDSITITKTSTTCDCMDVQIPKKILGPRDT
                     AHMAVTYNARIPGLFEKDVYVTYTGQDSTFKFTLKGHVKQ"
     gene            complement(4282..6237)
                     /locus_tag="PLG24_05880"
     CDS             complement(4282..6237)
                     /locus_tag="PLG24_05880"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HMZ24452.1"
                     /translation="MDKPRILWQPSEQQINETNLYKYQNYITNYTNIQFNDYHDLWQW
                     SIDNSETFWSSLFDYFGLIYDGSLVPAKLGYEMPDVTWFSNIRLNLAENLTRYRQDDK
                     IAIIEVSETGENTSYTWRQVINAAAAIQKQFDQWGLKPGDRVCGVLPNTFNTTAAFLA
                     VSASGLIWSCCSPEFGLESILDRFSQIRPGALLTVDDYYYGGKNFDLKEKNSMLVQNL
                     ITVKHVLNLSKERPDDARVEQYLSDLRILENNDLRFERLPFSAPLWVLYSSGTTGVPK
                     AITHCHGGVLLELQKYHAFHNDTRPNELFFWYSTTGWMMWNYLHGAWLMDASLLLYDG
                     SPGYPDVDFLWKLADKYKITHFGTSAPYILANKKLGIEINKKYNLSALRSIGSTGSPL
                     PPEGFDYVYQHIKSDVWLASMSGGTDVCTAFVGGNPMRPVYEGEIQCRTLGCPLFCLD
                     EDGQVIHDEEGEMVIMKPMPCMPVYFWNDPEKIRYKESYFEMYPGLWRHGDWIRITPR
                     DGVVIYGRSDATLNRQGIRIGTSEIYRSLDHIPEILDSLIIHIERRDGDHWMPLFVVL
                     MDGVHLDDGLKNKIKHQLRNDYSPRHVPDEIIQVSGIPYTLSGKKMETPVKRLLSGRP
                     KEKCFNVDTVRNPESLEFFVGLASRIN"
     gene            complement(6308..7327)
                     /locus_tag="PLG24_05885"
     CDS             complement(6308..7327)
                     /locus_tag="PLG24_05885"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HMZ24453.1"
                     /translation="MYNSILKPILFNLPAEEAHKMAMSAANLCQKLPFGDAFLKILAG
                     KTADDSIHLLGLSFRNRIGLAAGFDKDGHYIEVLKNLGFSHIEVGTVTPKPQPGNPRP
                     RLFRLIKDEAIINRMGFNNQGVHQLAERLRQLRYDKPIIGCNIGKNKDTPNDEAWKDY
                     TYCLKALHPYADYFTVNLSSPNTPGLRALQEKDPLIRILSELQNINQSFSTPKPILLK
                     VSPDNEKQVFDDIIQVSGQCGINGLIATNTTVERSGLKTSKSELKAIGAGGLSGKPLT
                     QKSLDIVKYLRTNMPQNMALIGVGGISSHEDFQNMKDAGADLIQIFTSFIYQGLSVVS
                     KIARG"
     gene            complement(7317..7802)
                     /locus_tag="PLG24_05890"
     CDS             complement(7317..7802)
                     /locus_tag="PLG24_05890"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HMZ24454.1"
                     /translation="MGTYCDLYNSPVGQFLLEASDRGILSCDFIDDMVDRPSSYLPNI
                     HTIRAAEWLDAYFSKQPLPKLDYDLSGTDFQLKVWDTIDQIPYGQTISYNDIVKNIPG
                     TSARAVGTATGRNPLAIIIPCHRVIGSDGKLHGYSGGIKRKIWLLAFEKEDSLFLPDV
                     Q"
BASE COUNT         2356 a         1492 c         1628 g         2408 t
ORIGIN      
        1 tttcaaagcc ttgctcagac ttgttcttgc agcttgagta gtgcgtacat tagcagcagt
       61 cgcaaccctc atgccaatga agcctgccaa agcagaactg aatgctccga tgacaaatgc
      121 aattacaata aaccagtgac tggtgttagc aatagtagaa agaattccca gcgctattcc
      181 tgcaatgaca acaaatactg ccaaaaccct gtattctgct ttcaaaaaag ccatcgcccc
      241 ttcccgaata tggccggcaa taatcttcat tttgccttca ccttcatctt gtttattaac
      301 ccagctgaat tggtatgcca tatataaaag tccaacgacc ccgaacaaag gcaataaata
      361 gagtaatgat tccattaaaa ttacgatttt cgttttatta aaaatatcaa atttgctttc
      421 gctaagcggc tgcaaagata acatttatcc ttactttcac aaacggtttg gagattatta
      481 tatataaatt gaaatgttat ataagatatt gaatatgacg tattatgttg aatcgatgaa
      541 aaatcatgtc tcttttcttg tacggctttt atgtttgatt tgggtactcg tagggtgcaa
      601 agggaagccg gagcaagcat attccggggt attgaaacct gcttccgata cacagacaga
      661 atatctaagc gctaataaga atggtactca agaaaaaaat atccatatac cggaatatgt
      721 atatgaaatt ttagtttata ttcgtaccca taataacgcg ccgaagggat atgtcggtgg
      781 aagaaccttt cacaacagag aacgtcattt gcctgagact gaagctgatg gaagtcgtat
      841 cagataccgg gaatgggatg ttcatccaaa ggttagagga cgaaacagag ggcctgaaag
      901 attgattacc tcaaagcgaa gcgcatatta taccaaagat cattataaaa catttattta
      961 catcaacgaa acttttttta gtaaaaaatg aaatatatcc gaatagcctc tccggatact
     1021 gaaatcaaaa gagggaataa caaaatcacc atagaagtta acggttctga agtccgggat
     1081 atcaaatctt tttatgagat actagttaac aatctcgaat ttcccggtta cttcggtgat
     1141 aatttggatg ccttgtatga tatgctgatt gatcttcagt ggcttaagca ggatactatt
     1201 gacataatca tttcggacta tgaggacttt cttacggacg aagtcgatga agacaaagca
     1261 gaaatcatgc ttcttctaga ggatgtatgc agggaatgga aggaaggtta ttccgatgat
     1321 gaagattggg aaatgaagaa agtcagggta tatgtcttat gtgatgaaat gacgggtaag
     1381 caaatatcac ggatgatagc cgatatgact gaagaggaat gagttgactt tagtgaatgt
     1441 tttttatctg aaactgagcc tcgacgccgt attcatcgtt atcagcccgg gaaacgctaa
     1501 agccggtgca ttccagtaat tggttataga agatgtgtgt aacaggtttt ccaaatatcc
     1561 cgcctgatgt tttatccata cttgggatat aaccacttct tgactcagta aatccacctt
     1621 tttgtttgaa atcctgaatt tcagcactcc atggaaccat attgaatact cctgtaacga
     1681 ttagtggtac ctgcattgct tcaattctcg agttgattat gtctaattca cccaaagcaa
     1741 tctcccggga gttggcatcc agttgagggg gaaaataagc ggtgaaaaca tcgaatcttt
     1801 tgttgttttt cagaagttga atcttcaaaa gaggaatgtc attataaaat atggtatctg
     1861 ctgtgacaat gggatattgt gaaagtacct ttagtccgta gacatcgatt ctgggaaggg
     1921 atattttata cgaaaaatta ggttcaacct ggttttcaat tgcaccttgc cattccggtg
     1981 tatattcctg gaaaatcaag atatcgtggt tttttttaga aacgtactgg aatacttttc
     2041 tagggtccga atcaatagag gaaagattta tcaaggcgag tcggagtttt tcttcctggt
     2101 tgggcagtgc ataacgaata cgggcattcg tagtttcttt caaaaaaaag gcaaaaatac
     2161 cagtacatat caaagcggct atcataaggc gttgttggct gataagaaga aagaacatgc
     2221 tcaacgccag gtagaagatc ataatttgat tggcaaaatc agagcttttg ccgacaaaag
     2281 gtaagtcagg ggtaattatg gtagcaatta cccctgacaa aatcagaaat aaaagtatat
     2341 tttcaacgac tttgttacta aagaagttac ttatactctt caggatcatt ctcagaattc
     2401 ttcagtccct gcaaagtgga atgcactttc aattgctgca ttttcatcag aatcagagcc
     2461 gtgtacagcg ttttcaccaa catttcttgc atacaaggct cttatggtgc ctggttcagc
     2521 cttggaaggg tctgtggcgc caattaattt cctgaaatcc tcaacagcat tatctttttc
     2581 caaaattgct gcaacaatag gaccggaaga catgaaagaa accaattcgc catagaaagg
     2641 tcgttccttg tgtacttcat agaactttcc cgccttggca ggtgataatt gtgtaagctt
     2701 cattgcgacg atacgaaaac cagctgcgtt gatttgtgcc aggattgcgc cgatatggcc
     2761 atcctgaact gcatctggtt taatcatggt aaatgttctg ttagttgcca tttattaaac
     2821 tttattaatt tgtattgagg cgcaaaatta aagtaaaaaa tcaaatccga aagaattgaa
     2881 tgattttgta tagatacgtc atgcatttta acatttattg ggaacgttta ttaaaaaaat
     2941 aggattaaaa agaattgaac attatttaaa tttataacaa gtgataaaac tacttttgaa
     3001 aattgcgatt cctgtaatct tttgtttgct ggtttataat tacttttttg gtacgccgga
     3061 agaaaagaat acctcaaagg ctgttttttc aaagacaaag gatcttacag tcagcattta
     3121 cgatcttttg aaatcagaaa aacacaaatt tgatcaaggt aagtatgaca aggcactgga
     3181 taagttaaat gaaatctatg atgccgccag gtcaaaaagt gatggtatgt cggaggctga
     3241 taaggctgaa ttaaaaaaat tggagtccga aaagcaagat ctgaaaaggg aaattgacca
     3301 gacaaagaag cttgacgcaa aatctgcgga tgaaaaaagc aagtcacttg atgaaaagtt
     3361 gatggagttg gttgataaga ccaataggtt gttgggtgaa taacagtgat ttatcgaacc
     3421 cggaaataac ttgtgcaact aaatgatcaa ataattttta caaaaaaata tcaattgtag
     3481 ttgtaaatat caagaatgat tttacttttg caactccaat gcgggtgtgg cgaaattggt
     3541 agacgtacca gacttaggat ctggcgccgc gaggcatggg ggttcgagtc cctccacccg
     3601 cacaaaattt tcccttttat tttctctatt ttaagcagca tcaaggtgta aagcctttga
     3661 tgacctgttt ttgcattttt attacgttgg tcaaacaata gtcaaccgtt aaaaacggag
     3721 ttttgagaaa atttaaacaa agcacccaag gtattattgg gttcccgaca gattaggctt
     3781 gataagaaaa cggttattgt ttgacgtgac cctttaaagt aaatttgaat gtagaatcct
     3841 gaccggtata ggttacatag acatcctttt caaaaaggcc gggaatcctt gcattgtaag
     3901 tcacagccat atgtgctgta tcccggggac cgagtatttt tttaggtatt tggacatcca
     3961 tacaatcgca ggtagtagac gtttttgtaa tggttatgct gtcaggtttg ttgttagtaa
     4021 tcactacctg aacagttata ggaatatctc tgggaatggc tccgacatcc tttacggtaa
     4081 aatccataaa gacaccttca gtatttttgt tatcaactgt ctcagtcttt gttccttcgg
     4141 ttgtatcacc gggtttttta tcctgttggc aaccggagaa tgaaattaac atggaagcga
     4201 aaattataat aaaatttttc atatttaatt ttttgcaaat atataccttc tgggcaaatg
     4261 aaagcttctt tttctatatc atcaattgat tcttgatgct aaaccgacaa agaattcaag
     4321 gctttccggg tttcgaacag tatctacatt gaaacatttt tctttaggtc gcccggaaag
     4381 tagtctttta accggtgtct ccattttctt accactcaat gtgtatggaa ttccggaaac
     4441 ctggatgatt tcgtcaggga catgccgggg actataatca ttccgaagct gatgtttgat
     4501 tttatttttt aatccatcat ccaggtgtac accatccatg aggactacaa acaagggcat
     4561 ccaatggtct ccatcccttc gttcgatatg aataatcagg ctgtcaagaa tctcaggtat
     4621 atgatccaga cttcggtata tctcacttgt acctatcctg ataccttgtc tgttgagtgt
     4681 tgcatccgaa cgaccataaa ttactactcc gtcccttggt gtgattctta tccaatcccc
     4741 atgcctccat aggccgggat acatctcgaa ataactttct ttataccgaa tcttttccgg
     4801 atcattccag aaataaaccg gcatacaagg catgggtttc atgattacca tttctccttc
     4861 ttcatcatga attacttgcc catcttcatc caggcaaaac agaggacagc ctaatgttct
     4921 gcattgaatt tctccttcat agactggtct catgggattt ccaccgacaa atgcagtgca
     4981 tacatcagtg cctccgctca tggacgcaag ccagacatca cttttaatgt gttggtatac
     5041 atagtcgaat ccttcaggtg gcaaaggtga cccggtagag cctatggatc ggagtgctga
     5101 tagattatac tttttattaa tctcaatacc aagttttttg ttggctaaaa tgtatggggc
     5161 actggtgcca aagtgagtta ttttgtactt gtcagccagt ttccagagga aatctacgtc
     5221 cggatatccg ggactaccat cataaagcaa tagtgaggca tccatcagcc aggcaccatg
     5281 caggtaattc cacatcatcc aaccggttgt ggaataccag aagaagagtt cgtttggcct
     5341 tgtatcattg tggaaggcgt gatatttttg caattccaat aaaactcctc catgacaatg
     5401 ggtaattgct ttaggaacgc ctgtagtgcc ggaactgtag agaacccaaa gcggagctga
     5461 aaaaggcagg cgttcaaaac ggagatcatt attttctaaa atccggagat ccgaaagata
     5521 ttgttcgacc cgggcatcat caggtctttc cttacttagg tttaaaacat gctttacggt
     5581 gatcagattc tggacaagca tcgaattctt ttcttttagg tcaaagttct tgccaccata
     5641 ataataatca tccacagtaa ggagggcacc gggtcttatc tgactaaatc ggtccagaat
     5701 gctttccaat ccaaattcag gagagcaaca agaccaaatc aatccggagg cactgacagc
     5761 gaggaatgca gctgtcgtat tgaatgtatt gggcaacaca ccacatactc tatcaccggg
     5821 tttcaacccc cattgatcaa attgtttttg tatggcagcg gctgcgttaa taacctggcg
     5881 ccatgtataa cttgtgtttt ctcctgtctc tgaaacttca ataatcgcga ttttgtcatc
     5941 ctgtcggtat cttgttaaat tctctgctaa gttcaaccga atattgctaa accaggtgac
     6001 gtcaggcatt tcatacccta acttagccgg aacaagtgag ccgtcataaa tcaaaccgaa
     6061 ataatcaaac aggctcgacc agaaagtttc tgaattatcg atggaccatt gccacaagtc
     6121 atgataatca ttgaattgta tattggtata attcgtaata taattttggt atttatacag
     6181 gttggtttca ttaatttgtt gttctgatgg ctgccatagt atgcgaggtt tgtccattga
     6241 atttcttttt attggttctt agtaagaaaa ctctctataa ggaatatatg ttcacttctg
     6301 agccatgtca tccccttgcg attttactca ctactgaaag tccctgataa ataaaggagg
     6361 taaatatctg tatgaggtct gctcctgcat ctttcatatt ttgaaaatcc tcgtgagaag
     6421 aaatgccgcc tacgccgatc agagccatgt tctgaggcat attggttcta agatatttaa
     6481 caatatcgag agatttttgt gtcaatggtt ttccactgag gccacctgcg ccgattgctt
     6541 tcagttcaga tttcgatgtt ttcaggccac ttctttcaac tgtagtattg gttgcaataa
     6601 ggccattgat accacattgt ccggaaacct gaatgatatc atcaaatacc tgtttttcat
     6661 tgtccggaga gacttttaaa agaattggct ttggggtact aaagctttgg ttgatgtttt
     6721 gaagttctga tagaatacgg atcaagggat ctttttcctg gagtgctctt aacccgggcg
     6781 tattgggcga gctaagattg acggtaaaat aatctgcata agggtgcaat gctttcaggc
     6841 aataggtata atctttccag gcttcatcat taggtgtatc tttgttttta ccgatgttgc
     6901 aacctatgat tggcttatca taacgtagtt gccttaatct ttccgccagc tggtgtacgc
     6961 cctggttgtt aaatcccatt ctgttgataa tggcttcatc tttgatgagt ctgaaaagac
     7021 ggggccgggg attaccgggt tgaggcttcg gcgttacggt acctacctca atatggctga
     7081 agccgagatt tttcaatact tcgatgtagt gtccatcctt atcaaagccg gctgccagtc
     7141 caatccgatt cctgaatgaa agtcccagca ggtgaattga gtcatcagcg gtttttccgg
     7201 cgagtatttt aagaaaagca tctccgaacg gcagtttttg acataaatta gctgcagaca
     7261 tagccatttt gtgggcttct tcagccggaa ggttaaataa aattggttta agtatgctat
     7321 tgtacatccg gcaaaaataa ggaatcttct ttttcaaaag caagaagcca gattttcctt
     7381 tttattcctc ctgaataacc gtgtaattta ccatcagaac ctatgacacg atggcatgga
     7441 atgataatgg ccagaggatt tcggccggta gcggtgccta cagctcttgc tgaagtaccg
     7501 ggaatatttt tgacaatatc attgtaggag attgtttggc cataaggtat ttggtcaata
     7561 gtatcccata cttttaattg aaaatctgtt cctgaaagat catagtcaag tttgggtaat
     7621 ggttgctttg agaaataggc atcgagccat tcagcggcac gaatggtatg aatatttggc
     7681 aggtaagagg atggcctatc aaccatatca tcgataaagt cacaagataa aattcccctg
     7741 tcagaggctt caagaagaaa ttgtccgaca ggggaattat ataagtcaca ataagtgccc
     7801 attggtaatt atgctgacac tgtgattgga ttgttgcctt cgagaggttt tggatctttc
     7861 ttcagttcag ggtgcaacaa ttcg
//
LOCUS       DAOQQN010000118         7837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3665_length_7837_cov_4.888075, whole genome shotgun sequence.
ACCESSION   DAOQQN010000118 DAOQQN010000000
VERSION     DAOQQN010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3665_length_7837_cov_4.888075"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /gene="rpsT"
                     /locus_tag="PLG24_05895"
     CDS             <1..291
                     /gene="rpsT"
                     /locus_tag="PLG24_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HMZ24455.1"
                     /translation="HWIKKDTFKLIFCMAHHKSSLKRIRQDAKKRIQNRYYKKSTRTM
                     IKKLRELTDKSEATTMLPKVISMVDRLAKHNNIHKNKAANLKSSLTRHVASI"
     gene            complement(474..1169)
                     /gene="trmB"
                     /locus_tag="PLG24_05900"
     CDS             complement(474..1169)
                     /gene="trmB"
                     /locus_tag="PLG24_05900"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HMZ24456.1"
                     /translation="MSNKNKLQRFAEIAVLPNVLENFDFHDPHVADTFGNRVDIKGHW
                     NRLMFERDLPIVLELACGKGEYTTHLAVADPEKNYIGIDIKGARIWKGATMAHDQGLK
                     NVAFLRTRIELIRSFFEKNEVSEIWITFPDPFLRDSKSNRRLTSNAFLDRYTGILVPG
                     AILHLKTDDPTLYKFSLKVLENREDYEIVEHTDDFYKDEMPTPETHLLTFYEQQHLAQ
                     GKKITYIKFRYQG"
     gene            complement(1219..3048)
                     /locus_tag="PLG24_05905"
     CDS             complement(1219..3048)
                     /locus_tag="PLG24_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ24457.1"
                     /translation="MRHSFMVTVGILMYSCLFAQSTDVLTDIMNNQLENYFVVNQKEI
                     NSGALEFSPAIWKDSLVFVANGRKIRGRSAAEGKKLFRIFKASISEDGTLKDDGQFSD
                     LYSEYNEGPLCFNHSGDKVYFTRNIKVVDEHDKTRLNLNIFYSERIEGQWSEPIALFV
                     PGADVNYCHPAISPDGMHLYFSSNRQGGYGNYDLYRVDNVDGSWSEPVNLGPEINSAS
                     NELFPTLLSRDILSFSSNRPGGTGGFDIYLAKIFRKTSIEVTRLPAPINSESDDMSLI
                     LTGDNSKAYFSSARQGGKGMDDIYLLQITPGTEPVVIQQTLVIVDSVRVQRLQDARCV
                     LKTLAGDVVADGVTDRNGSLQVSVTKGHQYMLEVESRNYLPYQSPLWGGENKTVRLQP
                     LPCLTVNGFALEQETNKLLRDAEIVWVNDCGEPEIKVVSDSIGRFELCVPPNCSGVII
                     GRKEEFNTVRVPVPALNENLTINVNFSMVMLSIVREPIRKGVKLILDNIYYNFNESTI
                     RPSTDRELNELAILMKQYPKMRINLIAHTDSRGDANANLTLSINRAMEAKKYLVEKGI
                     AEDRIETEGRGESQLRNRCKDGVNCTEEEHQYNRRIEVKILEI"
     gene            complement(3085..4104)
                     /locus_tag="PLG24_05910"
     CDS             complement(3085..4104)
                     /locus_tag="PLG24_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HMZ24458.1"
                     /translation="MKMKIGETGGGIFDLSGIKAYILNQKVLLVMVSVLLLSGTGFSQ
                     QERQYSQFMYNKLAINPAFAGALPSTTFNALYRNQWMGFEGAPTTQALSANFPFLRQR
                     VGLGANLIRSTIGISEMWTLSGMYAYRLPLGNGYISGGMEGSLRYYGVDYNSPSLRAD
                     QNLASDNALIVENTRAIVPNVGLGVYYSDKNFYVGFSVPRILRSRIDFKNDLLISREV
                     THFYLMTGYSLEVAESVSLTPQLNVKYVRNSPLTIDANMMVDFGMKYNAGISYRSGGV
                     YSSSGESIDILFGMKIVEPVFLGLAYDIQLGNLGKYSNGSIECFLRYSLPAKEDMEVQ
                     NPRFF"
     gene            complement(4180..>7837)
                     /locus_tag="PLG24_05915"
     CDS             complement(4180..>7837)
                     /locus_tag="PLG24_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ24459.1"
                     /translation="NAVSNINFDIPPGNVLSGINPCGSVPINQDGQVDSISFMFMQLK
                     YTTFSPPSYFATGATSIPYSELQPPYDAPKYKFNAGTTYFSYTISDISGNTDTCTIKV
                     EVRDTIAPEAVCKYATLRLHPSGIFPGKLTPEMIDGGSFDNCRIDTMFVTDRLYTCAD
                     IGTQPEVTLYVQDPVGLIDSCKTQILIEKALLEPGYSLGLCDKDTLRLFANMPDLNLF
                     DQYIYQWTGPNNFSSNVQNPVIPSANSNHSGSYRLQVSGFNGCGGEGVVQVFINDEIN
                     TPIISAKDTVVCDGSNIVLNTQTYSGNIKYRWYEGFAPDGVFIDSTIVPQLTLQRPPG
                     EYFFYVVVSENACVSNPSSSTSVEVVTNPVAIVDDASISICEGGSIRLESPLGTDYNY
                     QWTGPNGFKSNEQYPPVISPATLSNAGLYSLVVSLQNCKSAPAQVSVTVSPRPEKPTP
                     TSNSPVCSDSELVLTSNISLGIDTFVWKRPDGTLITTTSNPLTIDSVDQGANGLWTLT
                     LKSGGCASLESDAIKIEVTPNTTVEIHYEGPVCEGDSVRLETNDFPGAAYKWTGPNGF
                     TSTLADPTVKAAPGTYAVTIVTAAGCVFKGATTLDLRQKPQIISLTTNAVDCRFPDEI
                     ITFDYITNIPEQELSFDWRGPGGFMSTEAHPAVVTAQLVNGVYSVIATSRDGCVSDTA
                     KVTISGSVAPEKPIIQGRDRLCSGDTLTLSASNLPVVGKYYWETPNGTITTTSNQLTI
                     VNIQPSNGGAFSVIYDSGNCRSRPSDVFEVKVVKTPDKPVIVGDPTICLGDSIVLQIS
                     NAPDFQYNWAGPGGITSSDKKWVIFPSQQSNAGNYLLTLVNEGCVSKTSDPFALTIIL
                     PPPAPKIVDPAQEFCVSGTSQLITLCIDESTATPGASYAFYNTGRPKAIAGPTNALCA
                     NITNFSQLNDGVNVLYATATLNTCTSTQGIPVQITINFPPEEFAVTGKVKVACEGKQT
                     TLSAKRPDFSTGTWKSLTANTVIVDPSDPNTEVSGLSVGKNVFVWSLDYNNCKDFSRD
                     TMIVWVSDELKAVNDVYRTYEATGLQLDVLANDIFNVPVVLEVEDGPLYGTVSIKDGN
                     TLEYAPLPGKDYDVITYRICAAGCPQLCATAKVTIDIDALNDCRVPNIITPNNDGIND
                     FLRIPCLDSLVGNTSSITIFNEWGSQVFEALPYLNNFDGKYKGSDLPPGTYFYIFDKG
                     DRSPVQKGFLIIKR"
BASE COUNT         2122 a         1892 c         1651 g         2172 t
ORIGIN      
        1 cattggatta aaaaagatac ttttaaatta atattttgta tggcacatca caagtcatca
       61 ttgaagagaa tcagacagga tgctaaaaaa cgtatccaga acaggtacta caaaaagtca
      121 acacgtacca tgatcaagaa gctgagagaa ctgacagaca agtcagaagc tacaaccatg
      181 ttacctaaag taataagcat ggttgacaga ttggccaagc ataacaacat ccacaagaac
      241 aaggccgcaa acctaaagag cagccttacc aggcacgtcg cttccatctg attctgacat
      301 gataaagata tggccgggca gctatcagtt gtccggcttt tttgttttcc gccattacgg
      361 cctgacttcc ttatcttcac atgacaccac tccggttccc ttttactccc gggttgaagt
      421 aggtctttta tcaatggcaa aagttgacct gcaaatgata cattgatccg ggattatccc
      481 tggtatctaa atttgatata ggtaattttc ttaccctgag ccaggtgttg ctgctcatag
      541 aaggtcagca aatgcgtttc cggcgtaggc atctcatcct tgtagaagtc atccgtatgt
      601 tcgacgatct cataatcttc ccggttttcc aggactttga gtgagaattt gtacagggtg
      661 ggatcatctg tcttgagatg aaggattgca ccgggtacaa gaattcctgt atatctgtcc
      721 aggaaggcat tagaagtcag gcgccgattg gatttcgagt ccctcaggaa tggatcggga
      781 aaggtgatcc atatctctga aacttcattc ttttcaaaaa atgaccggat caattcaatc
      841 cgtgttcgca agaaggccac gttcttcaac ccttggtcat gtgccatggt agcacctttc
      901 caaatcctgg ctcctttaat atcaattcca atataattct tttcagggtc tgctactgca
      961 agatgggtcg tgtattcacc cttcccacat gctagttcaa gcactatggg caggtcacgc
     1021 tcaaacatca aacggttcca atgccccttg atatcaactc tattgccgaa ggtatccgcc
     1081 acgtgcgggt cgtgaaaatc aaaattctcc aatacattgg gcaggacagc tatctcagca
     1141 aatctttgaa gcttgttttt attcgacatg gtttggattt agaaggtgca aaggtaagca
     1201 atggctatgg atagctgctc atatttccaa tattttgact tcgatccttc tgttgtactg
     1261 atgttcttcc tctgtacaat tgactccatc cttacaccga ttgcgcagct ggctttcccc
     1321 tcttccctcc gtctcaatcc tgtcttcagc tattcctttt tccaccaggt atttcttggc
     1381 ttccatggcc ctgttgattg aaagagtcag attggcattg gcatctccgc gagagtcggt
     1441 atgggcgatt aaatttatcc tcattttcgg atattgcttc atcaatattg ccagctcgtt
     1501 cagttcacga tccgtagaag gccggatggt cgattcatta aagttataat aaatattatc
     1561 aagaatcagc ttcactccct ttcggatcgg ctccctgaca atagaaagca taaccatgga
     1621 gaaattgaca ttgatggtca aattctcgtt taaagccggg acaggaaccc gtaccgtgtt
     1681 gaattcttcc tttcggccaa tgatgactcc tgaacaatta ggtggaacac aaagctcaaa
     1741 tcggccgatg ctgtcggaaa caacctttat ctcaggttct ccacaatcat tgacccatac
     1801 aatttccgca tctcttaata acttattggt ttcctgttcc agagcaaatc cattcacagt
     1861 aaggcatggc aaaggttgca gcctcactgt tttattctca ccaccccaca aaggggattg
     1921 atatggcaga tagttccgtg actctacctc cagcatatac tggtgcccct tggtcacact
     1981 cacctggaga ctaccattgc gatccgtaac accatccgct acgacatcac ctgcaagggt
     2041 tttcaaaaca catcttgcat cctgcaaacg ctgaaccctt acactatcca ctatcaccag
     2101 tgtctgttgg attacgacag gctctgttcc gggagtgatt tgaagaagat aaatatcgtc
     2161 catgcctttt cccccctgac gtgcagaaga aaaatatgcc ttactgttgt ctccggtcaa
     2221 tatgagactc atatcatcac tttcactatt gatgggagca gggaggcgtg tgacttcaat
     2281 agatgtcttc ctgaatattt ttgccaaata aatgtcgaag ccaccggtac cgccgggcct
     2341 attggagctg aacgagagga tgtcccggga aagcaaggtt ggaaataatt cattggaagc
     2401 ggaattgatt tcagggccga gattgacagg ttctgaccaa ctgccatcga cattatccac
     2461 tctataaagg tcgtaattgc catatccacc ttgccggttg gaagagaaat acagatgcat
     2521 gccatccgga ctgatagcag gatgacaata attgacatcg gcgccaggca caaataatgc
     2581 tataggctca ctccattggc cttcaattct ttcagaataa aaaatattga gatttaatcg
     2641 ggttttgtca tgctcatcaa caaccttaat atttcttgta aagtagacct tatctcccga
     2701 atgattaaag cagagagggc cctcattata ctcggaatat aaatcggaaa actggccatc
     2761 gtccttcaat gtcccatcct cgcttataga tgctttgaat atcctgaata gcttttttcc
     2821 ctcagcggct gatcttcccc gaatcttcct tccattggca acaaatacca gactatcctt
     2881 ccaaatggcg ggcgaaaatt ccagtgcacc tgaattaatc tccttttgat tgacgacaaa
     2941 ataattttcc agttgattat tcataatgtc agtcaaaaca tccgtactct gagcaaacaa
     3001 gcacgaatac atcaatatcc cgactgttac catgaaactg tgtctcatgc aagatcccat
     3061 tctcaatcta aaaatcatca tttatcagaa aaaccttgga ttttgaactt ccatatcctc
     3121 cttggccggc aagctatacc tcagaaaaca ttcgatactt ccattggaat atttacccag
     3181 attgcccagt tgaatatcgt atgccagacc cagaaataca ggctcaacaa tcttcatccc
     3241 aaacaggata tcgatagatt ctccgctgga tgaatatact ccaccgctac ggtaggaaat
     3301 accggcgttg tacttcattc caaaatcgac catcatattg gcatctatgg tcagtggact
     3361 gttcctgaca tacttcacat taagctgcgg tgtcagcgag acactctcag ccacctccaa
     3421 cgaataacct gtcatcaagt aaaaatgtgt cacctccctc gatatcagca aatcgttttt
     3481 aaagtcaatc cttgacctta aaattctggg cacggagaat ccgacataaa aattcttgtc
     3541 ggaataatat actcccagtc cgacatttgg tacgatagcc ctggtatttt ccacgatcag
     3601 ggcattgtct gaggccaggt tctgatcagc acgtaatgac ggactgttgt aatccacacc
     3661 gtaatatctg aggctgcctt ccattcctcc actgatgtat ccattgccca agggtaatct
     3721 gtatgcatac attccactta atgtccacat ttctgagatg ccaatggtac tcctgatcag
     3781 gttggccccc agtccaaccc gctgcctcag aaaagggaag tttgccgaca atgcctgtgt
     3841 agtcggcgcc ccttcaaaac ccatccattg attacggtat aaggcattga atgtggtgga
     3901 aggcagggct cctgcaaagg caggattgat agccagcttg ttgtacataa actggctata
     3961 ttgcctttcc tgctgactga atccggtgcc ggacagtaga aggacagaca ccatcaccag
     4021 cagtaccttt tggtttaaaa tgtacgcctt gattccggat agatcgaata tgccaccgcc
     4081 ggtctctcca attttcatct tcataccatt gagatttctt tttgtatcat ttggaaatac
     4141 gttcgttttt tctgtattca ttgtttccat cacgtgactt taccttttaa tgattaagaa
     4201 tcctttctgg acagggctcc tgtctccctt gtcaaagatg tagaaataag tcccgggagg
     4261 caagtcactt cctttgtact ttccgtcgaa attgttcaga tagggtaacg cttcgaaaac
     4321 ctgacttccc cactcgttga aaatagttat gcttgaagta ttgcccacta agctatctag
     4381 acagggtatc cgaaggaaat cattgattcc gtcattattt ggtgtaataa tattcggaac
     4441 acggcaatca ttaagagcat caatatcaat agtcacctta gcagtcgcac ataactgagg
     4501 acatcctgct gcacagatcc tgtaagttat cacatcatag tctttgccgg ggagtggtgc
     4561 atattccagt gtattgccgt ccttgatact aactgttccg taaagtggcc cgtcttccac
     4621 ctccagcact accggtacat tgaaaatatc attggccagt acatccaatt gaagcccggt
     4681 tgcctcataa gtacgataga catcattgac cgctttcaat tcatcagaaa cccaaacaat
     4741 catcgtgtcc cgagagaaat ccttacagtt gttgtaatcc agactccata caaatacatt
     4801 ctttccaaca gataatccgg acacttctgt atttggatct gaaggatcaa caatcaccgt
     4861 attcgccgtc agagacttcc aggttcccgt agagaaatcc ggtcgcttcg ctgataatgt
     4921 tgtctgcttg ccttcgcagg caacctttac cttacctgtt actgcaaatt cttcgggcgg
     4981 gaagttgatt gtaatctgaa ccggtatgcc ttgggtcgat gtgcatgtat tcagggtagc
     5041 ggttgcataa aggacattga cgccatcgtt gagttgacta aagttggtga tgttggcaca
     5101 cagtgcattg gtcggtccgg caatagcctt gggtcggcct gtgttgtaaa atgcataact
     5161 cgcacccgga gtagccgttg attcatcgat acacagggta atcaattggc tagttccgct
     5221 gacgcaaaac tcctgtgcag gatctactat ctttggcgca ggtggtggta agataatcgt
     5281 caatgcaaag ggatcagatg tcttggatac acaaccttca ttgaccagcg tcaataggta
     5341 attaccggca ttgctttgct gtgaaggaaa tattacccat tttttatcac ttgaggttat
     5401 cccgccaggg cctgcccaat tatactggaa gtccggcgca ttgcttatct gcaatactat
     5461 ggaatctcca aggcaaatag tcgggtcacc cacaatcacc ggtttgtccg gagttttgac
     5521 aaccttcacc tcaaagacat cagaaggcct cgatcggcag ttgcctgaat catagattac
     5581 cgaaaatgca ccgccattgg aaggctgaat attgacaatc gtcagttgat tggatgtcgt
     5641 tgtgattgtt ccgtttggtg tctcccagta atatttgccc accacaggaa gattgcttgc
     5701 tgataaggtc agggtatctc ccgagcaaag cctgtctctg ccctgaatga ttggtttttc
     5761 cggtgccact gaaccggaga tagtgacttt ggctgtatca gaaacacatc catccctgga
     5821 agtggctatc acagagtata cgccgttgac aagctgagcg gtaactacgg ccggatgagc
     5881 ttctgtactc atgaatcctc cgggtccgcg ccagtcgaag ctcaactcct gctccggtat
     5941 attggtaata taatcgaatg tgatgatctc atccgggaac ctgcaatcaa ccgcattggt
     6001 tgtcagggat attatttgcg gcttttgcct cagatcaagc gttgtagctc ccttgaaaac
     6061 gcaccctgcc gcagtcacaa tggttacagc ataggttccg ggagcggcct taaccgtcgg
     6121 atctgccaag gtagaggtaa atccgttcgg accggtccat ttgtaggctg ctccgggaaa
     6181 atcatttgtc tccaaccgta cactatcccc ttcgcataca ggaccctcgt aatgaatctc
     6241 caccgtcgta tttggcgtaa cttcgatttt gatggcatct gattccaacg aagcacatcc
     6301 gccagatttt agtgtcagag tccacaaacc gttggctccc tggtcgacag aatctattgt
     6361 caatgggttg gatgtcgtcg ttatcaaagt gccatccggc cgcttccata caaaggtgtc
     6421 tattcctaag gaaatattgg aagtcaaaac caattccgaa tcggaacaca ccggagaatt
     6481 ggaggtaggt gtcggttttt caggtcttgg agagacagtc accgaaactt gtgccggagc
     6541 ggatttacaa ttttgcaaac ttaccaccag ggaatacaaa ccggcattgc tcagggtagc
     6601 gggactgatg accggaggat attgttcatt ggacttaaaa ccattcgggc ctgtccattg
     6661 gtaattgtaa tcggtaccaa gcgggctctc aagtcttatg ctaccgcctt cgcaaatgct
     6721 gatgcttgcg tcatcaacta tggctaccgg attggtcact acctcgactg aagtcgatga
     6781 tgaaggatta cttacacagg cattttcaga tacaacaaca taaaagaaat actcccccgg
     6841 cgggcgctga agagtcaatt gcggcacaat ggtagagtcg ataaacacgc catccggcgc
     6901 aaagccttca taccatctgt atttgatatt gcctgaatat gtctgggtgt ttaatacaat
     6961 gttgctacca tcacagacaa ctgtatcttt agcagaaatg atcggtgtgt tgatctcatc
     7021 attaataaac acctggacaa caccctcacc gccacagccg ttgaagccgg acacctgcag
     7081 cctatacgat ccggagtgat tggaattggc actcgggatg acaggattct gtacgttgga
     7141 cgaaaaatta ttgggacctg tccattgata gatatattga tcaaataagt tcaaatcagg
     7201 catattggca aacaaccgca aggtatcctt gtcacacagg ccaagactgt agccgggttc
     7261 caacaatgcc ttttcgatca gtatctgagt cttgcacgaa tcaatcaatc ctaccggatc
     7321 ctggacatac agggtcactt caggctgtgt acctatatct gcacaggtat atagtcgatc
     7381 ggtaacaaac atggtatcta tcctgcaatt gtcaaagctt cctccatcaa tcatctcagg
     7441 agtaagcttg cccgggaaga taccgctcgg atgcagacgt agagtggcgt acttacacac
     7501 agcctccggt gcaatagtgt ccctgacctc gaccttgatt gtacatgtat ccgtattccc
     7561 tgaaatatcc gaaatcgtat atgagaaata cgtagttcct gcattaaact tgtatttagg
     7621 agcatcgtag ggcggctgaa gttcactgta aggaatcgat gttgcgcccg tagcaaaata
     7681 ggaaggcggc gaaaacgtcg tatatttcag ctgcatgaac ataaaactaa tgctatccac
     7741 ctgaccatcc tggttgatgg gaaccgaccc acatgggttg ataccactca aaacattacc
     7801 cggaggtatg tcgaaattga tatttgatac ggcattg
//
LOCUS       DAOQQN010000119         7822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3673_length_7822_cov_5.603965, whole genome shotgun sequence.
ACCESSION   DAOQQN010000119 DAOQQN010000000
VERSION     DAOQQN010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3673_length_7822_cov_5.603965"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLG24_05920"
     CDS             <1..393
                     /locus_tag="PLG24_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ24460.1"
                     /translation="KVANAHAFIEQLEDGYQTIIGDRGMRLSGGQRQRLTIARAILKD
                     APIIIMDEATSALDTESERLVQDAINRLTHNRTGIIIAHRLSTISHADEIIVLQRGSI
                     AERGTHQELMQKGGYYRKLVEMQQLEKK"
     gene            748..2061
                     /locus_tag="PLG24_05925"
     CDS             748..2061
                     /locus_tag="PLG24_05925"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HMZ24461.1"
                     /translation="MDPLKELFKTKAFPVRDELKTVIKEHGEMKIAEVKISQLVGGMR
                     SIKSMLWETSLLDAEEGIRFRGYSIPQLIELLPKIEGDSEPLPEGLFWLMLTGDIPSR
                     DQVEWLSKEWERRSQVPDEVFAILDACGNDVHPMTMFSIGVLAMQPYSIFAKRYDEGF
                     SKADYWDATYEDTMNLIARLPHIAAYIYRKVFRNNEHIPSKKGLDWAANFAHMLGFER
                     DDFKSLMRLYLTIHADHEGGNASAHATHLVGSTLSDAYLSLSGGLNALAGPLHGLANQ
                     EVVNWINEMVEKIGTKKPTKEQIADYVNTTLAAGQVVPGYGHAVLRKPDPRFMEQKRF
                     AERYMPDDDYVNIVWKVFDVVPEILRGLGKVTNPWPNVDAHSGSILIHYGLTESSFYT
                     VLFGVSRAMGVLAQLCWSRALGLPIERPKSITFDNVKEFINNQKS"
     gene            2073..2465
                     /gene="gcvH"
                     /locus_tag="PLG24_05930"
     CDS             2073..2465
                     /gene="gcvH"
                     /locus_tag="PLG24_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HMZ24462.1"
                     /translation="MSNVPKELKYSKEHEWIRVDGNIGTVGITDFAQGELGDLVYVDI
                     DTVGQTIAKDEVFGAVEAVKTTSDLFLPVTGKILEVNPAITESGDDDPSLINSSPYDK
                     GWIVKIEIANPSELDSLLDSDAYEALIG"
     gene            2465..2872
                     /locus_tag="PLG24_05935"
     CDS             2465..2872
                     /locus_tag="PLG24_05935"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HMZ24463.1"
                     /translation="MAKLHIDRHRLFRVLARVWIITLTILMLLPGKSIPVVRWALNIP
                     IDKTIHFLSFFFLAAGFLLGYRWEDRSKRRILFVELLVYASIMEVIQHLLQPSRAFEM
                     ADMLANILGIITAFIGYYVLGKFIKKLTLNPPN"
     gene            3032..6658
                     /locus_tag="PLG24_05940"
     CDS             3032..6658
                     /locus_tag="PLG24_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HMZ24464.1"
                     /translation="MKRFFLLFLSLSLFSKISAQQIGWQPVSKEVAMGRSWASDENVP
                     LENLFKVDFNELNQSLLNIPVSAKVGFDMEMPMPDGKTQLYTVVSDPVMEADLQARYP
                     NIRTYKVISKETGGLAGRIGTSPEGFYGVLDINGKEVLINKVNRNDRSLYSVFRLVDG
                     INAVDMFKPMACGADFAEVKKVEASSMEDKIETRAVEVKMQHFRVGIATTSSFAAEIG
                     LTTVEQVLARVTQVINQVNLRYNKDHAMHLDLISNTTNLFNMTPEDDFFKQQNNGGQL
                     LGPSQEFFQMKLANKDYDFGQTWTQYCVDVGGVVSGSACNNQSKARGVSCGPSNVSYF
                     LQTVKHEMGHQFSASHTFNSCNGSTQQASQGAYEPGSGSTIMAYPGACGPDNIAGGAD
                     DYFHVISILHVRGHHQAYPECGTFAEDVNHMPEITLPKYNSDLLTIPKLTPYELIGSA
                     TDHNQDKLYYIWEEFDQGNGEPLGTNFESGPLNRTYPPLTNNSTRLIPRLNNIVNDVF
                     DIADRMPEATREMNWKFVVRDYNPNAGAVAIADFKFRVDSNAGPFKFSFPNKNTDSVL
                     TVGQYFEVKWDVANTDKEPVNTKFVDIFYSTDGGVNYKDTLVIGTPNDGAEYVTIPQK
                     AQNSRFKIKGHGSIFLDMSRKTLRVVEPDSAGFSYDVYPHNALLCPFEPQNFLIKGIS
                     WKGFDKPIKVELVDGWPSNTVIKLDKEIMNPGETLTLSIDTRDVTDAGFYTIRMIATA
                     EGQDTIWRYMEVEILPSKLEFTGAVNPLPGNTNESQVPSFAWNIIPGADRYEFQLASD
                     SKFENILHTYTGTAIAIEPQVVLPPGAIYYWRVRASNRCYDGPWSEYNTFQTILFECN
                     TINALGLPKAISSNVTAPPVDVLFDISNSTGVVADINVKNIDISHSDISKLKISARSP
                     SGKEINLMTGRCAGISGLYTTFDDVSPLEINCGLIKANKTFKPEEELSAFNGEPVQGN
                     WSIRVTTIRGGSSGKINNIDFDLCASVTTPPIIKVNNNLFKVNSDHSQVLENKDLLYT
                     CENTGDGNIWYIMLSEPVHGYFTLYGQEIQKGDRFTQGHLNDWGVQYVPYDSEYEGTD
                     KVDFLVRNDKFAYLAPEQFNIDLSKTHTVSTKPINKDISKLIKFYPNPTNGEVNIDLS
                     DIKILETGKLTVTDIAGKVVMQKPIPANTDRVVLDLGRMSAGVYFAGIYSGNYHAMQK
                     IIKQ"
     gene            6780..>7822
                     /locus_tag="PLG24_05945"
     CDS             6780..>7822
                     /locus_tag="PLG24_05945"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HMZ24465.1"
                     /translation="MKIIRLLAFNLLFISLYTTSRAQININWQTANPSSEQKAEISSM
                     LLLPQDARFYKASFLHTTLESVSGSEVVQVTLPIGMDGAEVKVHFVYDPVMGARDQQK
                     FKEIRTFKAFSTEGTPISGRAGISPTGFYGVFELNGKQIMIRPGAVEQDMYVVYDLGR
                     KLSMLDFSLLAGCGTETHLNEIKRNETVNLRSDDKRMRNFTIAISCTSGYADQVGRSE
                     GQVMASVVEVLNLLNYRYNVDFGIKLNLLDGTSQLFNLDPHTDYFVNETKGLELLQQN
                     QDFLDSLIDNSSYDLAQVFTRGCSDVGGVVWGRTCVSDTKARGVSCRTNDSDYFFTTF
                     KHEVGHQFSGGHT"
BASE COUNT         2337 a         1558 c         1839 g         2088 t
ORIGIN      
        1 aaggtggcca atgcccatgc cttcattgaa caattggaag atggttatca gactattatt
       61 ggtgatcggg gcatgcgcct cagtggtggc cagcggcaac gccttactat cgcccgcgca
      121 attctgaagg acgcaccgat cataatcatg gacgaagcca cctccgccct ggatacggaa
      181 agtgaacgat tggtgcagga tgcaatcaat cgattgacac ataacaggac cggaatcatt
      241 atcgcccatc gacttagcac catcagtcat gccgatgaga tcattgtcct gcagagaggc
      301 agtattgctg aaaggggaac ccatcaggaa ttgatgcaaa agggcggata ttataggaaa
      361 ctcgtggaaa tgcagcagct ggaaaagaaa taagaagaat aatcaggcat tccaggttgt
      421 caattccgga ggtaataaaa tgacggcgct atatttaacc cgggtttctg gcaaacaata
      481 ggaagttcat tccgtaagcc ccattatata aaggcctcgt tgtcctcaat ccgtcgggat
      541 gatttgggcc gctgacccga tcatccaatg atgcacgaca ttcatttcat tcttgtccga
      601 agaactaaaa gattactgga ttttggggga ttagaaacat tagtaaataa tgctaaaagt
      661 tgtatcttag cgacttcaat aattaaacaa taaagaccga aatggcgttt aatgactttg
      721 tttcaggtct aaaagattat acattatatg gatccattga aggagctttt taagacaaaa
      781 gcgtttcctg tcagagatga gttgaaaacc gtcataaaag agcacggcga aatgaaaatt
      841 gccgaagtga agatcagtca gcttgtagga gggatgagga gtatcaagtc aatgttgtgg
      901 gagacttcat tacttgatgc tgaagaggga attcgtttca gagggtattc gataccacag
      961 ctgatagaat tattgcctaa gattgaagga gattctgagc cgcttccgga aggcttgttt
     1021 tggcttatgc taaccggaga tataccctcg cgggaccagg ttgagtggct aagtaaggaa
     1081 tgggaaagaa gatcacaggt gcctgatgaa gtgtttgcca tacttgatgc ctgtggcaat
     1141 gacgtacatc caatgaccat gttcagtata ggggtattgg caatgcaacc gtacagcata
     1201 ttcgcaaagc gatatgatga aggttttagt aaagcggact actgggatgc aacgtatgag
     1261 gacacgatga atctgatagc ccggctgccc catatcgcag catatattta cagaaaggta
     1321 ttcaggaaca atgaacatat accatcgaaa aagggactcg actgggctgc taattttgcg
     1381 catatgcttg gatttgaaag ggatgacttt aagagcctga tgcgacttta cctgactatt
     1441 catgccgatc acgaaggtgg aaatgcaagc gcacatgcaa cgcacctggt aggatcaacc
     1501 ttgagcgatg cctatctttc actgagtggg gggcttaatg ctttggctgg gccactgcat
     1561 ggtcttgcca atcaggaagt ggtcaactgg atcaatgaga tggtggagaa aataggaacc
     1621 aagaagccta caaaggaaca gatagccgat tatgtcaata caacgctagc ggcaggacag
     1681 gtggtgccgg gatatggaca tgccgtattg cgcaagccgg atccgagatt tatggagcag
     1741 aagagatttg ctgaaagata tatgccggat gatgattatg taaatatcgt atggaaagtc
     1801 ttcgatgttg tcccggaaat tcttcgcgga ctgggaaagg tgaccaaccc ttggcccaat
     1861 gtcgatgcac acagtggctc gattctgatt cactatggtc ttacggaaag cagtttctat
     1921 accgtgctct ttggtgtcag ccgcgcaatg ggtgtacttg cacagctgtg ttggtcaaga
     1981 gccttaggct tgcctatcga aagacctaaa tccatcactt ttgacaatgt aaaagaattt
     2041 atcaataatc aaaaaagcta aaagatcaga ctatgagcaa cgttcctaag gaattaaagt
     2101 acagtaaaga gcatgaatgg atcagggttg acggaaatat tggcaccgtg ggtatcactg
     2161 attttgctca gggagagtta ggcgatttgg tgtatgtcga tattgacacc gttggacaga
     2221 ccattgcaaa agatgaggtt tttggcgccg tggaagcggt caagaccacg tcagatctct
     2281 ttttgccggt tacaggaaag atcctggagg tcaatcccgc aataactgaa agcggagacg
     2341 acgatcccag cctcattaat tcctctcctt atgacaaagg atggattgta aaaattgaaa
     2401 tagcaaaccc ttccgagctg gactccctgc tagacagcga tgcctatgag gcattgattg
     2461 gttgatggca aaattacata ttgatagaca tcgattattc agggtgctgg cccgtgtatg
     2521 gatcatcacc ctgactattt taatgctatt gccgggcaag agtattccgg tcgttcggtg
     2581 ggcacttaat attccaattg ataaaaccat tcattttctc tccttttttt ttctggcagc
     2641 cggattttta ctgggctaca gatgggaaga tcgcagcaaa cgcaggatct tattcgttga
     2701 attattggta tatgcatcaa taatggaagt aatacaacat ttgctgcagc ccagtcgtgc
     2761 ctttgaaatg gcagatatgc tggctaatat acttggaata ataacagcat ttattggcta
     2821 ttatgtttta ggtaaattta taaaaaaatt aacactaaac ccgcctaatt aaaagggttt
     2881 aattaagaaa gattccaaat aaaagctatt tttgtacctc gtttgtcaaa atgaaataac
     2941 tgataactcc ttttgtaaaa acagttgttt tttgccgcca cacaacttat tgaattgact
     3001 aaacttttat tcattttaaa ttacatttca catgaaaaga tttttcctat tatttctttc
     3061 acttagcctt ttttccaaaa tttcggcaca acagataggc tggcagcctg tttccaagga
     3121 agtggcaatg ggacgcagtt gggctagtga tgagaatgtc cccctggaaa acctttttaa
     3181 agtagatttc aatgaactca atcagtctct tttgaatatt cctgtatccg caaaagttgg
     3241 ttttgatatg gaaatgccaa tgccggatgg gaaaacgcaa ctttataccg ttgtctcaga
     3301 tcctgtaatg gaagctgatt tgcaggcaag atatcccaac ataagaacct acaaggtcat
     3361 ctcaaaggag accgggggcc tggcaggccg tatcggcact tccccggaag gtttttatgg
     3421 tgttctggat atcaacggta aggaagtact catcaataaa gtcaacagga acgatcggtc
     3481 tttatactct gtctttagac tggttgatgg aataaatgct gttgatatgt ttaagccaat
     3541 ggcctgtggt gctgattttg ctgaagtgaa gaaagttgaa gcttcctcaa tggaggataa
     3601 aattgagaca agggcagtgg aagttaagat gcagcacttc agggtaggaa ttgccacaac
     3661 atcaagcttt gctgctgaga ttggactgac cactgtagag caagtacttg cgagggtaac
     3721 ccaggttatc aatcaggtca atctcagata caacaaggat catgcaatgc acctcgacct
     3781 gattagtaat accaccaacc tgtttaacat gacacccgaa gacgacttct tcaaacaaca
     3841 aaacaatggt ggccagctac tgggcccgag tcaggaattt tttcagatga aattggcgaa
     3901 taaggactat gatttcggcc agacctggac acagtattgt gttgatgtgg gtggtgttgt
     3961 gagcggttct gcctgtaata atcagtctaa ggcacggggg gtcagttgtg gaccatccaa
     4021 tgtaagctac tttcttcaaa cagtgaaaca tgagatggga caccaattca gtgccagcca
     4081 cacgttcaac tcctgtaatg gctccaccca acaggcatct cagggagcat atgagccagg
     4141 cagtggaagc actattatgg catacccggg ggcttgtggc cctgacaata tagccggcgg
     4201 agctgatgac tactttcatg tgatcagtat tttgcatgta cgcggccatc atcaagcata
     4261 tcccgaatgc ggaacttttg ccgaagatgt caatcatatg cctgaaatca ccctgccaaa
     4321 atacaattcg gatcttctga ctatacccaa acttacaccg tacgaactta ttggaagcgc
     4381 tactgatcac aatcaggata agctttatta catctgggag gaatttgatc aaggaaatgg
     4441 tgagccattg ggaacgaatt ttgaatcagg cccgctcaac agaacctatc cgccattaac
     4501 caataatagt accaggctga ttccgcgact gaataatatc gtaaatgatg tgtttgatat
     4561 cgctgacaga atgcctgaag ccacaagaga aatgaactgg aaattcgtag taagggatta
     4621 taatccaaat gcgggcgcag tggctattgc agactttaaa ttcagggttg attcaaacgc
     4681 aggacccttc aaattcagct tccccaacaa aaacaccgat tctgtcctta ctgtaggtca
     4741 atacttcgaa gtgaagtggg atgttgccaa tacggataaa gaacctgtga acaccaaatt
     4801 tgtggacatt ttttattcta ctgatggggg tgtgaactac aaagatactt tagttatagg
     4861 tacacccaat gatggtgcag aatatgtaac aattccccaa aaagctcaga acagtcgatt
     4921 taagattaag gggcacggca gtatcttcct cgatatgtcc agaaagacct tgcgtgtcgt
     4981 ggagccggat agcgcggggt tctcctatga tgtatatccc cataatgcac tattatgccc
     5041 gttcgaaccg caaaacttcc ttataaaagg tatttcctgg aaaggatttg ataaaccgat
     5101 aaaagtggaa ttggttgacg gatggccttc caacacggtt attaagctag acaaggagat
     5161 tatgaatccg ggagaaacgc tgacgctttc aatcgacacc agagatgtga cggatgccgg
     5221 tttctatacc atcagaatga tagctacagc agagggtcag gatacgatat ggcgatatat
     5281 ggaagtggaa attttacctt caaaactgga atttaccggg gctgtcaatc ctttgccggg
     5341 taacacgaat gagtcacagg ttccgtcctt cgcctggaat attataccgg gtgcagatcg
     5401 atatgagttc cagttggctt ccgattccaa atttgaaaac atactacata cctacaccgg
     5461 cacggctatt gcaatagagc ctcaggtggt acttcctcct ggcgcaatct attactggag
     5521 ggtaagggca agtaacagat gttatgatgg cccatggtcg gaatacaata cctttcagac
     5581 catactgttt gaatgcaata caatcaatgc tctgggcctt ccaaaggcga tatcgagcaa
     5641 tgtcacagct ccgccggtgg atgttctctt tgacataagc aacagcacag gtgtggttgc
     5701 cgatatcaat gtcaaaaata ttgatatcag ccactccgat atttcaaaat tgaagatttc
     5761 cgcaaggtca ccaagtggca aggaaattaa cttaatgaca ggcagatgtg caggtatttc
     5821 agggctttac acaacttttg acgatgtgtc accactggaa atcaattgcg gtttgatcaa
     5881 agcaaataag acattcaaac ccgaagagga actttcagcg tttaatggcg agccggtaca
     5941 aggcaattgg tccattcgag tcacaactat cagaggggga tcctccggaa agatcaacaa
     6001 tattgatttt gacctttgtg caagtgttac cacaccgcct atcatcaaag tcaacaataa
     6061 tttgttcaag gttaattctg accatagtca ggttctggaa aataaagatt tgctgtacac
     6121 ctgtgaaaat accggtgatg gaaatatctg gtacatcatg ctatcagaac cggtacatgg
     6181 atatttcaca ctatacggac aggaaattca aaagggtgat cggtttactc aaggccacct
     6241 gaacgactgg ggggtacaat acgtacccta tgattccgaa tatgaaggaa cagacaaggt
     6301 cgacttcctg gtgcgtaatg ataagtttgc ttatttggca ccggaacaat ttaatataga
     6361 cctgagtaaa acacatactg tatcaacaaa gccgatcaat aaggatattt ctaaattgat
     6421 caaattctat ccgaatccta caaatggtga ggtgaatatt gacctttcag acatcaaaat
     6481 cctggaaaca ggtaagttga cagtaactga tattgcaggt aaagttgtaa tgcagaaacc
     6541 gattcctgcc aatacggata gggttgtact tgatcttggc aggatgtctg caggagtcta
     6601 ttttgccggc atttattcag gaaattacca tgcaatgcaa aaaataatca agcagtaaat
     6661 tttattcaaa ccagtatgtc atcagaggct ttttcggatt ccttggaagg agggcgtttt
     6721 cgggaaagcc tctgtttttt tctgaatcgc tccattttta attaaaaacc gaacatagta
     6781 tgaagatcat cagacttctt gcatttaact tactattcat ttctttatat acgacaagcc
     6841 gtgcacaaat caatataaac tggcagacag caaacccatc ctcagaacaa aaagcagaga
     6901 tttcgtccat gttgcttttg ccacaggatg ccaggtttta caaagcctcc ttccttcata
     6961 ccacgctgga gagtgtttcc ggatcagaag ttgtacaagt aaccttacca attggtatgg
     7021 atggtgctga agtaaaagtt cacttcgttt acgaccctgt catgggagca agggatcagc
     7081 aaaaattcaa ggagattagg acatttaaag cattttcgac cgagggcaca ccaatatcag
     7141 gaagagccgg tattagtccg acagggttct atggagtatt cgaattgaat ggaaaacaga
     7201 tcatgatcag gccgggtgct gttgaacaag atatgtatgt tgtatatgat ttggggcgta
     7261 aattgtccat gctcgacttt agtcttcttg ccggttgtgg aacagaaacc cacctgaatg
     7321 agattaaaag gaacgagact gtcaatttgc ggagtgatga caaaagaatg cgcaacttta
     7381 ccattgcaat cagctgtact tcgggatatg ccgatcaggt gggtcggtcg gagggtcagg
     7441 ttatggccag tgtcgttgaa gtacttaatc ttctcaacta tcggtacaac gtagattttg
     7501 gcattaagct caatttgctt gacggaacat cccaattgtt taatctggat ccccataccg
     7561 actattttgt caatgagacg aaggggttgg aactgttgca gcagaatcag gatttcctgg
     7621 atagcctgat tgataattca tcgtatgatc tggcacaagt gtttacacga gggtgcagcg
     7681 atgtgggcgg tgtcgtgtgg gggcgtacct gtgtcagtga taccaaggca cgtggtgtat
     7741 cctgtaggac caatgatagc gactatttct ttacgacttt taagcatgaa gtgggacatc
     7801 aattcagtgg cggtcacacc tt
//
LOCUS       DAOQQN010000120         7810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3685_length_7810_cov_5.917215, whole genome shotgun sequence.
ACCESSION   DAOQQN010000120 DAOQQN010000000
VERSION     DAOQQN010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7810
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3685_length_7810_cov_5.917215"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="PLG24_05950"
     CDS             complement(<1..935)
                     /locus_tag="PLG24_05950"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HMZ24466.1"
                     /translation="MKRIIFLGYSLLMVQLTIFGQGSWRKANDAPVFNRIDDIAFIND
                     SVALMGQDGKIYRSDDGGDNWILIGNLPHKAYVRSIEFLNDSTGFIGTIFDTKNGVAL
                     YRTDDGGKNWHSVSDRVQGGMYGICGLDHIGSTIIGVGIYSEPGRFYISRDGGDTWKS
                     YLIPESAALVDCVMLDENTFLVAGNSPDGRKGLILKSKDGGETWAEVAKSQYAMSFCW
                     KLSLRDDGLGLGSIEEAPTAFITHDHGDTWEEVLVDAQNKDTLRFGGAYFINEKLGWL
                     GIQWGMGTWETRDGGKNWKPIPFGGAINKIRGTNKG"
     gene            1393..2019
                     /locus_tag="PLG24_05955"
     CDS             1393..2019
                     /locus_tag="PLG24_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMZ24467.1"
                     /translation="MKKKYKTEQEDFWAGIFGDEYIHRNKSEEYLASNLNFFSKALNK
                     INKPESLIEFGANIGMNLRAIKLLFPKINIFGIEINGNAAEILSQFIGKEKVFNGSIL
                     EFVPEQKFDVVLIKGVLIHVDPDILHEVYAKLYESSKKYILIAEYYNPSPVTLNYRGH
                     SNRLFKRDFAGDMLDQYPDLRLIDYGFCYRRDNLFPQDDITWFMLEKS"
     gene            complement(2075..5086)
                     /locus_tag="PLG24_05960"
     CDS             complement(2075..5086)
                     /locus_tag="PLG24_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HMZ24468.1"
                     /translation="MKNLMPLMTIAILISSHFNLLIAQPGNDELAWKISNYKEKRSQN
                     IHRIVEISDGNLLAVGYSSNHSEGGRDILLLKIDRNGKLLKAQTLGGRYDDECLSAKY
                     MPSGKILLCGYSKNKGLSQGIILLADEDLNTKLLYMDEKNENSLFKGIEVDRQGQIFV
                     CGQAGSKLVVKKFSPKGEFINQLYLTDEEGSNANALIFNRDGELYCTGSMANRKEFNR
                     SLMFLLKLDSNLNKIGLWNFPGKGFYEGNDLKENNVGELVIAGGGRHKNRGGSTPAIA
                     VVSTNGDLIRMETYPSFIDGIARGVAITPCGDILIAGYGSDLRNAQRTALFSVRINPG
                     NNYGTVWGPVFGGGSFSDEYNDVVCTSDGSVVFSGFTNNVLNQDYYIYTLKPVRRDLE
                     ELTILKNVEIMDSENEALNKNMETDLKITLRNNLSKDIKTFKVKIEIENNDKKGLILP
                     ESILVDRLRGGEEKEVNIPLVITDDFRDDYIDLKFIVEGGDRHQYNSRKAKIGVIGTY
                     ESSLVLVVDSSRADIADKDRIVLPVSLRNLGDAPALVRDMDFRFNENVSLADDYEAPV
                     NTVIQVGEELRFNLICKVDSLFADDYIVANIKLRELSGYRIQIETPRSLTLRKMIPVI
                     DDNSQLYDQPIILSNEATEISFTNMAETVRLIDRKNYHVNFDCNLTPSEIASNFKFII
                     NGVEIAKDSLKIGYKENNYSVAVPLTLGDNELILERDAGVRDTLGKINVASSNVNYYI
                     YIIGTDLRDEDNMPVVRYSIKDVNDFYNKIKLLGKSKNTKVNIKLFNTFSLTTSNNLK
                     GVFNELLARKHKFKANDVMIIYWVGQGFATAKGDIYVKGSELLKNRKNKFMPENYSFP
                     LTSIYIPYFLGELPCKKIMLLDIFNKEDPESTGNDDDEGQDYSLIDQTVSAIKTFRNF
                     SYILSGNPGQKSYELQKVENGAFTYAVIQALEGNKGNLIGDNVMALSDFFENISRSIP
                     DLVVKNGYKTWKQNPMYIKSRPEEDVVIFYK"
     gene            complement(5089..6459)
                     /locus_tag="PLG24_05965"
     CDS             complement(5089..6459)
                     /locus_tag="PLG24_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HMZ24469.1"
                     /translation="MMGQTPPKIVFRKNIKLKPQPVKQEKKNKLSFYIKWSVWSLVTF
                     VALFIFYRIGVFGKPEISPKKSTVSPPALTDTLESAQESSEDSIYSIIYNSNFFNYRI
                     VPDILEEDMNADDFFGMFHIHPEKKAKAEMLLGANARFTEGTRIVTLYSKDDEEIPLY
                     IYIEPDPSRYRYISFEKDIPEISNYIKRNLTIETEKRKIVIGKDALENITEQAPAELL
                     GRISHVMSWKKDIGKLDEGDVVKVAYEGLYYNKIFMGVGKLMALEWKGKEDSLKAFLV
                     SGEYDNEGRSVYCTEKGRPLKEAFRKSPVAYGRISSRFGIRLHPVRKVVKAHNGTDFA
                     SPEGSPIFAIGDGVVTVAAFKKANGNYIKIMHDKTYTTQYLHMSRFKPGIVAGKRVRQ
                     GDVIGYVGHTGEATGPHVCLRLWKNAVQVDFLKEKFPQQGKIKAFDAKVFQSARDYYS
                     KQLENI"
     gene            complement(6465..6695)
                     /locus_tag="PLG24_05970"
     CDS             complement(6465..6695)
                     /locus_tag="PLG24_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24470.1"
                     /translation="MENKTIQRASLESLDELSGFNTGESEGSFLKPPEFNLTAKLHNT
                     GMSPYEDRPVDDVGNRFAFGRSADSTSKKNIT"
     gene            complement(6701..7696)
                     /locus_tag="PLG24_05975"
     CDS             complement(6701..7696)
                     /locus_tag="PLG24_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMZ24471.1"
                     /translation="MKLPVTFGFLTTCILFNLVIFSSECQAQQLPLFTQYREVQGLIN
                     PASISHDYFLEKYNANLGITYRNQWTDINGHPVTAAIKADYIVVPANSSFSLLTGAYY
                     LSDRAGLTNRQSAYVRLGSIYSQNPSESGFGLGINIGQVWNSFNFGEVKLTDPQDINI
                     DKVSNFNYLDLGIGTYYYKKLKSGPLKNDQFYVGVSVPQIFNQDYVIKNGTNEFVVKR
                     VPHIYAHAGYYKTMSETMYIAGSFWYRYVHGGGSIADINARIYLFDKLWFGAGASTAG
                     TLHVEAGTILGKYFNFNDKFNLKIGYASDFNLGYLAKNLGLSHELNLILFLDTVN"
BASE COUNT         2299 a         1744 c         1377 g         2390 t
ORIGIN      
        1 cctttattag ttccacggat tttattgatg gccccgccaa aaggaatcgg cttccagttc
       61 ttaccaccat cgcgggtctc ccaggtgccc attccccact ggataccaag ccagcctaac
      121 ttttcattga tgaaatatgc tccgccaaat ctcaatgtgt ccttattctg tgcatcgacc
      181 aggacttcct cccaagtatc accatgatca tgagtaatga aggcagtagg cgcttcctca
      241 atggagccca agcccagacc atcatcccgg agcgacaatt tccagcaaaa gctcatagca
      301 tactgagact tggccacttc tgcccaggtc tctcctccgt cctttgactt caggatgaga
      361 cctttcctgc catcaggact gttacctgcg acaaggaagg tgttttcatc caacatgaca
      421 caatccacca gtgcggcact ttccggaatt aaataacttt tccaggtatc tccaccatcc
      481 cttgaaatat aaaaacggcc cggttccgaa taaattccta caccgataat agtacttccg
      541 atgtgatcca gcccacagat accatacatg cctccttgca ccctatcact gaccgagtgc
      601 cagtttttac ccccatcatc tgtacgataa agtgccacac cattctttgt gtcaaagatt
      661 gtgccgataa atcctgtact gtcattgaga aattcgatgc tacgtacata ggccttatga
      721 ggaagattgc cgataagtat ccagttgtct cctccatcgt ccgagcggta tatctttccg
      781 tcctgaccca taagggccac agagtcattg atgaaagcaa tatcgtcaat ccggttaaac
      841 acaggtgcat cattggcctt acgccacgaa ccttgtccaa aaattgtaag ttgaaccatc
      901 aaaagactgt agcccaaaaa aataattctt ttcattgtca tccattcatt gggttggaaa
      961 atatatcctg cgaatcacgg cgcaaattta gagaaaaacg ttgatttgat tgacggataa
     1021 agaaataagt cggcaatagg gataacgatg tgctaatcct gattggcttg cctgatatgc
     1081 gtccggataa cgggaaattg acaatttaca ccttccttta aattctttac aattattcat
     1141 tgaaaaatta cttttaggct acaaaaacca atccaacacc attgattaaa tactaaaggc
     1201 gcataataac aaatcgaata tgttggaatt ttgtacattt ggtcttgcat gctgtattcc
     1261 gctttcgata tgaaaatttg ggtatttgat tgtatgaata tggaatattg aaatacatgt
     1321 tgccgagaaa taaaaaatgg caccaggtga ttggaaaacg aaataatcat attaaaacga
     1381 acggtccgaa aaatgaaaaa aaagtataaa accgaacagg aagacttctg ggccgggata
     1441 tttggggatg aatacataca caggaacaaa agtgaagaat acctggcctc caacctgaat
     1501 ttcttttcaa aggcattgaa caaaatcaac aaacctgaat ctctgataga gtttggagcg
     1561 aacattggaa tgaaccttcg ggcaattaag cttctgttcc cgaagatcaa tatattcggt
     1621 atcgaaataa atggaaatgc ggcggaaata ttatcccaat ttatcggtaa ggagaaggta
     1681 ttcaatggat ctattctgga atttgttccg gaacaaaagt ttgatgtagt gcttatcaag
     1741 ggagttttga tccacgttga tccggatata cttcatgaag tatatgccaa gctctacgaa
     1801 tccagcaaga aatatatcct aatagcagaa tactacaacc cctccccggt cacattgaat
     1861 tatcgtgggc atagcaatcg gcttttcaag cgggactttg ccggtgatat gcttgatcaa
     1921 tatccggatt tgagactcat cgattatggc ttttgttaca gaagggataa tttgttccct
     1981 caggacgata taacgtggtt tatgctggaa aaatcctgaa atttttaaac aggtattttg
     2041 cctgacagat attcaataat ccaatataat catattactt gtaaaaaatc acgacatctt
     2101 cttccggtct ggatttgata tacatagggt tttgtttcca ggttttataa ccattcttga
     2161 caaccaaatc cggaattgat ctggaaatat tttcaaagaa gtcagatagg gccattacgt
     2221 tgtctcctat cagattccct ttgttccctt ccaaagcctg aataacggca taagtaaagg
     2281 ctccattttc gaccttctgt aattcatagc ttttttggcc cggatttccg gaaaggatgt
     2341 aactgaaatt tctaaaagtc ttgatggcgc tgacagtttg gtcaatcagg ctgtaatcct
     2401 gcccctcatc atcatcattt cctgtgcttt ccggatcttc cttattaaaa atgtcaagga
     2461 gcataatctt tttgcaaggc aactctccaa ggaaatatgg aatatatatt gatgtcaaag
     2521 gaaaactata gttttcgggc ataaatttgt tcttcctgtt ttttaaaagc tccgacccct
     2581 ttacatagat atctccttta gctgttgcaa agccttgccc aacccaatat atgatcatta
     2641 catcatttgc cttaaactta tgtttccggg ctaataattc attaaaaacg cctttcaaat
     2701 tattagaagt agtcaggcta aaagtattga acagtttaat gttgactttt gtattttttg
     2761 acttgccaag taattttatc ttattgtaaa aatcattgac atccttgata ctgtaccgga
     2821 ctaccggcat attgtcttca tctctgagat cggtcccgat aatgtatata taataattga
     2881 cgttactact ggcaacattt atcttgccaa gagtatctct gacaccggca tctctttcca
     2941 atatcaattc attatctccg agagtcaacg gaacagcaac tgaataatta ttttccttgt
     3001 aaccgatttt taaggaatct tttgcaattt ctactccatt gatgataaac ttgaaattag
     3061 aagctatttc tgaaggagtt agattacaat cgaaattgac atgataattt tttctgtcga
     3121 tcagcctgac agtttctgcc atattggtaa atgatatttc ggttgcttcg ttgcttaaaa
     3181 ttatcggttg gtcataaagt tgtgaattat catcgatcac cggaatcatt ttccttaatg
     3241 taagactcct gggggtctct atttgaattc tatatcctga caattctctc aatttaatat
     3301 tcgcaacgat ataatcatct gcaaaaagag aatctacctt acaaatcaga ttaaatctaa
     3361 gttcctcgcc tacctgaatg acagtattaa caggagcctc ataatcatcc gcaaggctca
     3421 cattttcatt aaaccggaag tccatatccc taaccaaggc aggtgcatcg cccaaattac
     3481 gaagggagac aggcaataca atccgatctt tatccgcaat atcagctctg gacgaatcta
     3541 ctacaagaac cagggatgat tcgtatgtcc cgatcacgcc tatctttgct ttccgggaat
     3601 tgtactgatg ccggtctcca ccttcgacaa taaacttcaa atcaatatag tcatcccgaa
     3661 aatcatctgt aataaccaat ggtatattga cttccttttc ctctccaccc cgcagtcggt
     3721 caaccaatat cgactccggc aaaatcaacc cttttttatc gttattttct atttctattt
     3781 ttaccttaaa tgttttgatg tcttttgaca agttgtttct gagtgtaatc ttcaggtcgg
     3841 tctccatatt cttattcaat gcctcattct ctgaatccat gatttcgaca ttcttaagaa
     3901 tcgtcaattc ctccaaatca cgacgtaccg gcttcaaagt atagatataa taatcctgat
     3961 tcaatacatt gttggtaaaa cctgaaaaaa caacactgcc atcggaagta catacaacat
     4021 cgttgtattc gtctgaaaaa ctgccacctc caaaaacagg cccccaaact gtgccataat
     4081 tattaccggg attgatccga acagaaaaga gtgctgtcct ttgagcattt ctcaaatcgg
     4141 aaccataccc ggcaatcaga atatctccac aaggagttat agcaacaccg cgtgcgattc
     4201 catcgataaa ggaaggatat gtctccatcc tgatcaggtc tccattggtt gaaacaactg
     4261 ctattgcagg agtagaaccc cctcggtttt tgtgtcgtcc tccacctgca atcaccaact
     4321 cgcctacatt attttccttc aaatcatttc cttcgtaaaa acccttacct ggaaaattcc
     4381 ataatccaat tttatttaga ttggaatcca attttaaaag aaacatcaaa gacctgttga
     4441 attcctttct gtttgccata cttcctgtac aatacagctc tccatctctg ttaaaaatca
     4501 aagcattggc attagaacct tcctcatcag tcaaataaag ctggttgatg aattcccctt
     4561 tagggctgaa ttttttgact accaacttac tgcctgcctg tccgcaaacg aagatttgac
     4621 cctgccggtc aacctcaatt cccttgaata gtgaattttc attcttttca tccatataca
     4681 gcagcttagt attcaaatct tcatctgcca acaagattat accttgtgaa agacccttat
     4741 ttttcgaata accgcataac agtatttttc ctgatggcat atacttagct gaaagacact
     4801 catcatcata acgtccaccc agggtctgcg cttttaacaa tttgccattc cggtctattt
     4861 tcaataaaag aatatctctt cccccttcgg aatggttgga cgaatagccc acggctaaaa
     4921 gattaccatc gcttatctca acaattctat ggatgttctg gcttcgtttt tctttgtagt
     4981 ttgatatttt ccaagctaat tcatcattac ctggctgggc gatcagtaag ttgaagtggg
     5041 atgaaatcaa aatcgcgatt gtcatcaatg gcattaaatt tttcatattc agatattttc
     5101 caattgtttt gaataataat ctctcgcact ctgaaatact tttgcatcaa aggctttgat
     5161 tttgccttgt tgaggaaatt tttccttcag gaaatcgacc tgtacagcat tcttccacaa
     5221 tctcagacaa acatggggac cggtagcctc accggtatgg ccaacataac caataacatc
     5281 accttgcctg actcttttcc cggcgacaat tcccggctta aatctggaca tgtgcaaata
     5341 ctgggtggtg taggtcttgt catgcatgat cttgatataa tttccattgg ctttcttaaa
     5401 ggcagcaaca gtaacaacgc catctccgat tgcaaagatg ggagaccctt ccggagaggc
     5461 gaaatccgta ccattgtgtg ctttgactac tttacggacc ggatggagtc ttattccaaa
     5521 ccgggaagag attcttccat atgcgaccgg tgactttctg aaggcctctt taagggggcg
     5581 ccccttctcg gtgcaataga ccgacctccc ctcattatca tactcaccgg aaacaagaaa
     5641 ggctttcaga ctgtcctctt ttcccttcca ctcaagtgcc atcaatttac cgacccccat
     5701 aaatatttta ttatagtaaa gaccttcata agcgacttta accacatcac cttcatccaa
     5761 tttcccaatg tcctttttcc agctcatcac atggcttatt cttcccaata actcagcagg
     5821 ggcctgctct gtaatatttt ccaaagcgtc tttacctatg acaatctttc tcttttccgt
     5881 ctctatagtc agattgcgtt ttatatagtt tgatatttcc ggaatatctt tttcaaagga
     5941 aatgtatctg tatcttgaag gatccggttc gatatagata tataagggaa tttcctcatc
     6001 atccttgctg tataaagtta ctatcctcgt cccttcagta aatctggcat tcgcgcccag
     6061 cagcatttcc gcctttgctt tcttctcagg gtggatatga aacatcccga aaaaatcatc
     6121 tgcattcatg tcttcttcga gtatatcggg tactatccgg taattgaaaa aattggaatt
     6181 gtatatgatg gaataaattg agtcttcgga tgattcctga gctgactcca aagtatccgt
     6241 caatgcaggt ggactaacgg tagatttttt tggggaaatt tcaggtttcc cgaaaacccc
     6301 tattctgtaa aaaataaaca atgccacaaa agtaaccaaa ctccatacac tccatttgat
     6361 ataaaacgaa agcttgtttt ttttctcctg cttgacaggt tgtggcttaa gttttatatt
     6421 ctttcgaaaa actattttag gcggagtctg acccatcatt catgtcaagt aatattcttt
     6481 ttgcttgtac tatctgcaga ccgaccgaat gcaaaacggt tgcctacatc atcaaccggc
     6541 ctatcttcat atggagacat cccggtattg tggagtttag ctgtgaggtt aaattcagga
     6601 ggttttaaga aactcccttc agactccccg gtgttaaaac cggataattc atccagggat
     6661 tccaggcttg ctctttgaat agtcttgttc tccatagtat tcaatttacg gtatccaaaa
     6721 acaggataag attcaactcg tggctcaggc ccaggttctt agcaagataa ccaagattga
     6781 aatctgaagc atacccaatt ttcaagttga atttatcatt aaaattaaaa tatttaccca
     6841 ggatagttcc ggcttcgaca tgaagggttc cggctgtcga cgccccggca ccgaaccaca
     6901 acttatcaaa cagatagatg cgggcattaa tatcagcgat ggatccccca ccgtggacat
     6961 atctgtacca aaaacttcct gcaatataca tagtttcaga catagtctta taatatcctg
     7021 catgtgcata gatatgcgga actctcttga ccacaaattc attcgttccg ttcttgataa
     7081 cataatcctg attaaatatt tgtggaaccg atacaccgac atagaattga tcgttcttta
     7141 aaggcccgga ttttaatttt ttgtaataat aggtgccgat gccaagatcg agatagttaa
     7201 agttacttac tttgtcgatg ttgatgtcct gtggatcagt caatttgact tcgccaaaat
     7261 taaatgaatt ccatacctgg ccaatattaa tacccagccc aaatccactc tcagaagggt
     7321 tttgcgaata aatagagccc aatctaacat aagcagactg acgattggtc aaccctgcac
     7381 gatccgacag gtaataggca cctgtaagca ggctgaagga ggaattggcc ggaacaacga
     7441 tatagtcagc cttgatggct gcagttaccg gatgtccatt gatatctgtc cactgatttc
     7501 tatatgtgat accaagattg gcattatact tttcaagaaa atagtcgtgg ctaatggagg
     7561 caggattaat caagccctga acttcacgat attgcgtaaa aaggggtaac tgctgtgcct
     7621 ggcattccga tgaaaatatc acgagattga aaagaataca ggtggttagg aatccaaaag
     7681 tcactggtaa tttcataatc tttatattta aagtttaaag taaattcttg acctgccaat
     7741 gctgatcaat tctgtatatt ttaatagttc agaacatcac ttcttaaacc cattcccttt
     7801 aaaccggtga
//
LOCUS       DAOQQN010000121         7755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3730_length_7755_cov_6.167532, whole genome shotgun sequence.
ACCESSION   DAOQQN010000121 DAOQQN010000000
VERSION     DAOQQN010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7755
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3730_length_7755_cov_6.167532"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="PLG24_05980"
     CDS             <1..1114
                     /locus_tag="PLG24_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24472.1"
                     /translation="DAYNLKNDAGNAVTNYEYAMEMDPTNAVPHYKIGTAYIRAKRYE
                     FGVPELRKAIETDPSYALAYKDLGKYYYDVDKYQEAKENYRKYMTMIGTPTLAEKVQY
                     ANILFLNKDYSEALPLLEQARVEDKANKYPNVLRMMGYAYHETHENNKAISALNNFLA
                     NRDTTKILSQDYLYLGKIQAELGQDSLSRLNYLQAFEMDSTLDSEIKVIADTLFNQKK
                     YDPAGMLYMAIAKSTNLAADYFYATRADYLAGNYSRGIEAAEGIINKLPNEVEGYLWK
                     ARHQALLDTPNKGAAAIDPYLQVVEKGKVDPSKYSKQMLEALNWLTVYYINIKADYAK
                     AQEYNDQALEVDANNEQVMKLFNFLLEATKPKSGKK"
     gene            complement(1219..2112)
                     /locus_tag="PLG24_05985"
     CDS             complement(1219..2112)
                     /locus_tag="PLG24_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HMZ24473.1"
                     /translation="MYVVPFTIPKLDTRSFHFQEDILPHFYRHLHRHQEVQMTLILKG
                     SGTLVAENRMHVFTKGDFFLLGSGQSHLFREDEDALNSLTKSGIHTLNLFFAPQGIIR
                     PLFDLPEMTVIIKWLGSASSGLKLINADVKVLEHHFRQVQNTEAADRLAAFIRMLQYL
                     ASDKQWHPLADVSPVKYSETEGMRMDDIYQYCMRHFKREISLDEIAGIAYMTPQAFCR
                     YFKKHTLKTFTEFVNELRINEACRLLVSDKHRTISSIAYQCGFNHAVTFNRVFRDIMK
                     MSPTEYLKNINAINKEEKLSK"
     gene            complement(2270..4447)
                     /locus_tag="PLG24_05990"
     CDS             complement(2270..4447)
                     /locus_tag="PLG24_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HMZ24474.1"
                     /translation="MKKLFILFLAISSSALLIAQGQKSFDLNDVFRKGTFRTKSLPGF
                     AYMKDGKSYIINKGDALEINHVLDGKSLGTYISSEQWIALAGDNSAGIESYQFSEDES
                     KVLIVTGSEQIFRHSSKGKTFVFDKKKGSGVVVFDGKPVSNPTFSPDGMKVGFTCENN
                     LYYMDLTTNTSTQVTNDGKWNFIINGMCDWVYEEEFSFTRAYEWSSDSRKIGFLRFDE
                     SKVPEYSMEYYRDEMYPIPFSFKYPKVGEDNSTLEVKIYNLDTRNTVTIDQIPDKEIY
                     YPRIKWTRDPNKLCVFKMNRHQNNLELLLADASSGKTTILMKEDNKYYIEINDYLTFL
                     KDGKHFIWVTDKGGFYQAFLHDMNGNRINAITQANYDLSNIYGYDENTKTLYYQLIAP
                     TPMQCEVYKVGLDGKKPVKLSIKDGNNAAQFSPTFEYMTLIYSNINTPTSWTLYDKKN
                     KIVRVLEDNQAAIDKFKEFKAGKAEFFSFTTSEGVQLNGYQIKPSNFDPNKKYPVFMY
                     LYGGPGSQQVVDRWGGGQSWWFKMIAESGYIVACVDNRGTGGRGEEFRKMTYMQLGHY
                     EVIDQIEAAKYLGSLPYVDAGRIGIFGWSYGGYMSSLCLLKGNDVFKAALAVAPVTNW
                     KWYDSIYTERYMRTYKENPDGFKDNSPIYFADRLKGNYFLAHGIADDNVHFQNSVEMN
                     RALIKAGKQFEFHMYPNSNHGIYSEGATMHLYNAMTKFIMDKI"
     gene            5484..6122
                     /locus_tag="PLG24_05995"
     CDS             5484..6122
                     /locus_tag="PLG24_05995"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ24475.1"
                     /translation="MGIAERKSKQKEELRQNILDAAKRLFLKEGYHATSIRKIAKEIE
                     FSPTTIYLYYKDKAEIAHALHIEGFKLLGRQFGLLMHIDDPFERIKVMGRIYMQFSLE
                     NTDFYELMFVLKEPMDHIKECGMEEGWEEGDQAFNLLMQNVLACQEAGYFKSFDPAKI
                     ALIAWSTLHGLCTLNLHGHLAFVKTYKGEFTHIEDLFTSTFNTFVAILEQLK"
     gene            6155..7558
                     /locus_tag="PLG24_06000"
     CDS             6155..7558
                     /locus_tag="PLG24_06000"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ24476.1"
                     /translation="MITALSNRSVFRRSKSIVVIILVGFILSASCAHSQRSILDDYII
                     QAIGQSPEIHQSNLELQKAGISRDFAANCRLPTVGFQLGYQTAHGGRNIDLPIGDMLN
                     GVYSTLNQITGTPSFPMLENQRINFLPSNFYDAKIQTTIPIYNPEVRHNIDLSENKIV
                     SANLDIRIRKRDLAFRVKSAYFDYLMAVEAVKIYRQALSLAQESRRVNERLLKNGKGL
                     PAYVIRSDAEIESISADISGAEMRVQNSASMFNYLLNRPENSPIDTMFDAKVALDAGR
                     LLATLPPDISRREEIKNLQNASVLYKNVIKIHKSAALPRLNGIINVGSQAENWQFDSQ
                     SRYFLLGLQLEVPIFSAFKVRQKTAIAQLELGQNESRTEYASRGLSLAAGVAINNLRS
                     ALESLDASQKQLEAASTYRRLIEKGYAEGVSTFIETIDARNQWTTAQLAQKIRLYKVL
                     QAVAAVERETASYNIDQ"
     gene            7567..>7755
                     /locus_tag="PLG24_06005"
     CDS             7567..>7755
                     /locus_tag="PLG24_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24477.1"
                     /translation="MNRLHKSTILLLTLLIACSPKKQEHSQIPETIIPVKTIAVVKQE
                     LNPDIYVSGQFTTEDESVL"
BASE COUNT         2352 a         1719 c         1548 g         2136 t
ORIGIN      
        1 cgatgcctat aacctgaaaa atgatgcggg aaatgcagta accaactatg aatatgccat
       61 ggaaatggac cctaccaatg ctgtccctca ctataaaatc ggaactgcct acatcagagc
      121 aaaaagatat gaatttggcg tgccggaact gagaaaggct attgagacag atcccagtta
      181 tgctctggca tacaaggatt tgggtaaata ctactatgat gtcgataaat accaggaagc
      241 taaagaaaat tataggaaat acatgacaat gatcgggact cctactttgg cagaaaaagt
      301 acaatacgcc aacattctat tcctcaacaa agattacagc gaggcattgc cgctgcttga
      361 acaggcccgt gtggaagata aggccaacaa atatcccaat gtcctcagaa tgatgggata
      421 tgcgtatcat gagactcatg aaaataacaa agctatcagc gcgttaaata actttcttgc
      481 aaaccgggat acaaccaaga tattgagtca ggactatctg tatctcggaa agatccaggc
      541 agaattaggt caggatagtc tttcccggct gaattatctc caggcctttg aaatggactc
      601 aacgcttgac tccgaaatca aggttattgc agacacattg ttcaatcaga aaaaatatga
      661 cccggcaggc atgctgtata tggctatcgc caaatcaacc aatctggcag ccgattattt
      721 ctatgcaacc agggcagatt atctggccgg taattattcc cgcggaatag aagcagctga
      781 aggaatcatc aataaactgc ctaatgaggt cgagggatat ctgtggaaag ccagacatca
      841 agctttattg gatacaccaa ataaaggtgc tgcagccatc gatccttacc tgcaagtggt
      901 ggaaaaggga aaggtcgatc catccaaata cagtaaacaa atgctggagg ccctcaactg
      961 gctgaccgta tattatatca atatcaaggc agattatgca aaagcacagg agtacaatga
     1021 ccaggctctt gaagtcgatg caaataacga acaagtaatg aagttgttca acttcctgct
     1081 tgaagcaaca aagcctaaat caggcaagaa ataataagac gaaaaatatt tgattttcga
     1141 agaataaaaa aggcggcccg gggtcgcctt ttttattcgc tggccgtcaa gaatcacccg
     1201 ccatccatct ccaaatcctt atttgcttag tttctcctcc ttgttgatgg catttatatt
     1261 tttaagatat tccgtcggcg acatcttcat aatatcgcga aagacccgat tgaaggtcac
     1321 tgcatgattg aatccacatt ggtaggcaat acttgaaata gtacgatgtt tgtcagatac
     1381 cagcaacctg catgcttcat ttattctgag ttcattgaca aactccgtaa aagtttttag
     1441 tgtgtgtttc ttgaaatagc ggcagaatgc ctgaggggtc atgtatgcaa tcccggcgat
     1501 ttcatccaat gatatttcac gtttgaaatg tcgcatacag tattggtaga tatcatccat
     1561 acgcattccc tctgtctccg aatatttgac aggacttaca tcggcgagtg ggtgccattg
     1621 cttgtcactg gccagatatt gcaacatccg gataaaggca gcaagcctat cggcagcttc
     1681 tgtattttgt acctgcctaa aatgatgctc caacactttt acatcagcat tgatcaactt
     1741 cagcccggag gatgcggatc caagccactt gatgattact gtcatttcgg gcaggtcaaa
     1801 taaaggtctg atgatcccct gcggtgcaaa aaatagattc agggtatgga tcccgctttt
     1861 ggtcaacgaa ttcaaggcat cttcatcttc tctgaacaag tgcgattgac cggaacccaa
     1921 cagaaagaag tcacctttag taaaaacatg catcctgttt tccgctacca aggtcccgct
     1981 acctttgagg atcaatgtca tctgtacttc ctgatgtcga tgcaggtgtc tgtagaaatg
     2041 tggcaatata tcttcctgaa agtgaaaact cctcgtgtcc agcttcggta tagtaaaggg
     2101 gacgacgtac ataaacgaat tttaaccaca aaaatacaat atcttatatt gtgtagttaa
     2161 aattcgttta tagtcatttg gggctgttca gtttctgaaa ttgcattaag cccattaata
     2221 aatgaaatca tactgcacaa aatcacacaa ctaaataaac gggcagatgt caaattttgt
     2281 ccatgataaa tttagtcatg gcattataca gatgcatggt agctccttcg ctatatatgc
     2341 cgtggttgct gttgggatac atatggaatt caaactgctt cccggccttg atcaaggcac
     2401 ggttcatctc tacactgttc tggaagtgta cgttatcatc agcgatgcca tgtgccagaa
     2461 agtaattgcc cttgagccta tcggcgaaat aaatcgggct gttatccttg aaaccatccg
     2521 gattttcttt ataagtacgc atataacgtt cggtataaat agaatcatac catttccagt
     2581 tggtgaccgg tgccactgcc aatgctgctt tgaacacgtc gttgcctttc aacaggcaga
     2641 gtgatgacat gtatccgcca taagaccagc caaaaattcc gatgcgaccg gcatcaacat
     2701 atggtaatga cccaaggtat ttcgcagctt ctatctggtc gatcacttca taatgtccca
     2761 actgcatata ggtcatttta cggaactctt ctccacgacc tccggtacct cgattgtcaa
     2821 cacatgcaac aatatatccg ctctccgcaa tcattttaaa ccaccaactc tgaccacctc
     2881 cccatcgatc cactacctgc tggcttcccg gccctccata aagatacata aatacaggat
     2941 acttcttatt cggatcaaaa ttacttggct taatctggta tccattaagt tgcactcctt
     3001 cacttgtggt gaaagagaag aattccgcct ttcctgcctt aaactccttg aatttgtcga
     3061 ttgccgcctg attatcttcc aatacccgga ctatcttgtt ctttttatca tacaaagtcc
     3121 agctggtagg tgtattgata ttggaataaa tgagtgtcat atattcaaat gtaggagaaa
     3181 actgagcagc attgttacca tccttgatag atagctttac tggcttcttc ccgtccagtc
     3241 ctaccttgta cacttcgcac tgcatgggag tgggtgcgat taactgataa tacaaggtct
     3301 tggtattttc atcatagccg tatatgttgg aaaggtcata attggcctgt gtgatggcat
     3361 tgattcggtt gccattcatg tcgtgtagaa aggcctgata gaatccgcct ttatcggtga
     3421 cccagatgaa gtgcttccca tccttgagga aggtcagata gtcattgatc tcaatataat
     3481 acttattgtc ttccttcatc aggatagttg tcttaccgct gctggcatcg gcgagaagca
     3541 gctcgagatt gttctgatgg cgattcatct tgaatacgca cagtttattc ggatcacgtg
     3601 tccatttgat gcgcggataa tatatctcct tgtcagggat ctggtcgatg gtgacagtat
     3661 tgcgggtgtc cagattgtat atcttgacct ccagggtgga attatcctcc cccaccttcg
     3721 gatatttgaa agaaaaagga ataggataca tctcatcccg gtaatactcc atagaatact
     3781 caggcacctt agactcgtca aagcgaagga agccaatctt ccgactgtcc gaagaccatt
     3841 cgtaagccct tgtaaaactg aattcttctt cataaaccca gtcacacata ccgttgataa
     3901 taaaattcca tttaccatca ttcgttacct gagtggaagt attagtggtc agatccatat
     3961 aatacaaatt gttttcacag gtaaagccta ccttcattcc atccggtgaa aaagtcggat
     4021 tgctgaccgg tttgccgtca aacacaacaa ctccactgcc ctttttcttg tcaaagacaa
     4081 aggtctttcc tttggaactg tgtctgaaaa tttgctcaga gcccgtaaca atcaacactt
     4141 tactttcatc ttcgctgaac tgataggact cgataccggc actgttatct ccggccaagg
     4201 caatccattg ttcgctgcta atgtaagtgc ccaaactctt tccgtctagg acatgattga
     4261 tttccaatgc atctccctta ttgatgatat aggatttgcc gtctttcata taggcaaatc
     4321 ccggcaagct tttagtccgg aatgtgcctt tcctgaaaac atcattaagg tcgaatgatt
     4381 tctgaccttg agcgatcaac aaggcagaag aagagatagc cagaaataaa ataaataatt
     4441 ttttcacttg cacagtcaat tttgattatc aatgttaaaa atagaaaatc gaacgtaaga
     4501 taaaaaaaaa tatcgatcaa gctttatcct gccctgctaa attgaaagat ttggaagatg
     4561 agagccgtga gaggcagaaa ccattacata acggtatcgg atattgcctt acttgctaca
     4621 cccttgatcc gctttgatta aattcatggt atttgatttt cccttgatca tgaatattat
     4681 tttcggatga atgtgattac tacacaacag tgctttctat tatttgtgga atattcacct
     4741 caaagctgta ttgcccatac aagtctgata ccatcgatat tgcaacaggc gccatcataa
     4801 ccttatcgga tcagtcgtcg ggcggccttc cgtctaataa ggccattcgt ctaacccaaa
     4861 agccctaatt gttaagacct gctaataaaa tataaattac tccaaaagtg aacacaaaac
     4921 agtaagtcat ctattccttc aaattaaaat cttgtctctg ttataacagt aaaatctcct
     4981 gatcaggatc aaatgagtta cttccaatat aggatatact gaccagaagg cccccacagt
     5041 acatattgaa cttataaaag gcatgaatgc cgttggatat gcccaagcat cactaattag
     5101 ctctacagcc atagctcacg agtcaacaag aatcatcttt atctcttgaa acccggctct
     5161 gacctcattt cctatcggga gtatatgccc tgattgacca acataaaaag atagcgaccc
     5221 attccttttg attcaattct tccactcttt tcatcccgtt tatgacaatt tcaagcgaaa
     5281 actcttttca ggtttcactg caactaaaca taaatgacca acacaaaaaa acagaccgtt
     5341 gtacaatatt tataaataga aaaatggtaa ggccgggttg aacattcagg gaaaccggac
     5401 catctaaaaa actcaagaaa aaatttataa aaaaggacat atatttacac tgttatttat
     5461 atttgcactg ttatttaatt tttatgggaa ttgcagaaag aaagtcaaaa caaaaggagg
     5521 agcttcgcca aaacatcttg gatgctgcca aaaggctctt tcttaaagaa ggttatcatg
     5581 ccacatcaat acggaagata gccaaagaaa tagaattcag tccaacgaca atttacctct
     5641 attataagga taaggcagaa atcgcccacg ccttgcacat cgaaggtttt aagttgttag
     5701 gaaggcaatt tggcttgttg atgcatatcg atgatccatt cgagcgaata aaagtaatgg
     5761 gacgtatata tatgcagttt tctctggaga ataccgactt ttatgaactg atgtttgtct
     5821 tgaaggagcc tatggatcac atcaaggaat gtgggatgga agagggttgg gaagagggag
     5881 accaggcatt caacctgttg atgcaaaacg tcctggcatg tcaggaggca ggttacttta
     5941 agagctttga tccggctaaa atagccctga ttgcctggag tacattgcac ggcttgtgca
     6001 ccttaaacct tcatggtcat cttgcctttg tcaagaccta taagggggaa tttacgcaca
     6061 ttgaagattt gtttacgtca accttcaaca catttgtagc aatcctcgaa caattgaagt
     6121 aatttttttc aaacaatggt taacactgta aactatgata acagcattaa gcaataggtc
     6181 tgtattcagg agaagcaaat caattgttgt aataatctta gtgggtttta ttctatccgc
     6241 atcctgtgcc catagtcaaa ggtcgattct ggatgattac attatacagg caattggcca
     6301 aagtcctgaa atccatcaaa gcaatctcga gcttcagaaa gctggcattt cccgtgattt
     6361 tgccgccaat tgcaggttgc ccacagttgg atttcaattg gggtaccaga cagcgcatgg
     6421 cggtaggaac atcgatctgc ccattggcga catgctcaat ggtgtgtact cgacgctcaa
     6481 tcaaattacc ggcacaccct ccttccccat gcttgagaat caaagaatca actttcttcc
     6541 ctccaatttt tatgatgcca agattcagac aacgattccg atttataatc cggaggtaag
     6601 gcacaacatc gatctgtccg aaaataaaat cgtctcagcc aatctggata tccgtattcg
     6661 taagagagac cttgcattcc gggtaaaaag tgcctatttc gattatctga tggctgtcga
     6721 ggccgttaaa atatatcgac aggctctcag tctggcacag gaaagccggc gggtcaacga
     6781 aagattgctc aaaaacggta agggccttcc ggcatatgta atacgatccg atgctgaaat
     6841 cgaaagcatc agtgcggaca tttcaggtgc tgaaatgcgg gtccaaaatt cagcctcaat
     6901 gtttaactac ttactcaaca ggcccgaaaa cagccccatc gatacgatgt ttgatgccaa
     6961 ggtggccctg gatgcaggcc gcctgctggc aacacttcca ccggatattt ccaggcgtga
     7021 ggagatcaaa aacctgcaaa atgcctcagt tttatacaaa aacgtgataa agattcataa
     7081 aagtgccgca cttccacgac tcaacggaat aataaatgtt ggatctcaag ctgaaaactg
     7141 gcaattcgac tcacaatccc gatactttct actaggacta caattggagg tgccaatatt
     7201 ttctgctttc aaagtcaggc aaaaaacggc tattgcacaa ttggagttgg gtcagaatga
     7261 aagtcgaacc gaatatgcat ccaggggcct ttctctggct gccggagtag ctatcaacaa
     7321 ccttcgctca gcactggaaa gcctggacgc ttcacaaaag cagctggaag cggcttccac
     7381 atataggcgt cttattgaaa aaggatatgc cgaaggtgtc tccaccttta tagagaccat
     7441 agacgcccga aatcaatgga ctaccgctca gcttgcccaa aagataagac tttacaaggt
     7501 gcttcaggca gttgctgcag tagagcgaga aactgcatcc tataacattg accaataatt
     7561 ttcaacatga atagactaca taaatccact atcctgctac tgactctttt aattgcatgc
     7621 tcacccaaaa agcaggaaca ttctcaaatc cccgagacca tcattccggt aaaaaccata
     7681 gcagttgtca agcaggaact gaaccctgac atctatgtat caggccagtt taccactgag
     7741 gatgagtctg tgttg
//
LOCUS       DAOQQN010000122         7606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3841_length_7606_cov_5.624553, whole genome shotgun sequence.
ACCESSION   DAOQQN010000122 DAOQQN010000000
VERSION     DAOQQN010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3841_length_7606_cov_5.624553"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PLG24_06010"
     CDS             complement(<1..390)
                     /locus_tag="PLG24_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24478.1"
                     /translation="MNNKDIEKRGLKLEDGVLHEADHVEYSRRRFLKSFGLAGGLGFM
                     LGGLPVSASTLTRWSMPPILEKSDRILIMIRLKGGNDGINTIIPLDQLSTYHNARPSI
                     GYQDNVMIQLNDQIAMSPHLAGLKRLWD"
     gene            complement(422..1870)
                     /locus_tag="PLG24_06015"
     CDS             complement(422..1870)
                     /locus_tag="PLG24_06015"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HMZ24479.1"
                     /translation="MADCNLAPLSPYVPTAEKPWNEQRVKHLYARIGYGITAAEIPDI
                     LSKNPVEYIESLVDEAFNLPVFPKPDWADWKPGQFNEEDGTEHYIIYTQFVTDWINEK
                     LKRPLRDKIALFWSNVLVTKFEDYYYAPFLYQYVTNLQRNALGNYRTFIREMGLEHSM
                     LIFLNGFQNNKWQPNENYARELFELFTLGLDNGYTENDIKETARAFTGYNGADLENYI
                     IYFTPFTHDNGLKTIFGETANFDYNGVIDLLFEKKGDLICRHICGKIYRHFVNAEGND
                     NIIQQLADIMLANDFELLPVFKALFCSEHFFDATEFNTHIKSHIEYFTGIIKQFNYTL
                     NYEEGNAMIWYSSSLGQTYFDPVDVAGWKEDKSWIDSGSLLVRWLYSEWMYGGLIWQN
                     QDFVVSYLKNLTNNSNDVEFVVRTVVDQFFVSGLASQADYDRLLIAFKADLPENYYEQ
                     GLWNLDWEKDFICWQTNLLLNEMARLPDVTLC"
     gene            complement(2439..3206)
                     /locus_tag="PLG24_06020"
     CDS             complement(2439..3206)
                     /locus_tag="PLG24_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HMZ24480.1"
                     /translation="MQHLNVSLVQYDIIWEDSNANLEYLDTLLETARGSDLIILPEMF
                     NTGFSMDAATVAQKDDGQAVSWLCIRSGELETTIMGSVAIREEGRYYNRCYIASEGEI
                     IAQYDKRNCFTYAGEEKIYTSGNSQVTFEVKGWKVKPLICYDLRFPLWCYNRQEADLM
                     VFVANWPESRIIHWDVLLQARAIENQAFVIGVNRLGSDGTGKAHNGHSAIVGPYGNYV
                     TGPLTKAGVSHARLDPSEISRYRAMFHLLDEERMKSE"
     gene            complement(3194..4330)
                     /locus_tag="PLG24_06025"
     CDS             complement(3194..4330)
                     /locus_tag="PLG24_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HMZ24481.1"
                     /translation="MTDKLAKYGASIFSTMTAKAIQHGAINLAQGFPDFPVDERLIDA
                     VTEAMKSGYNQYARPSGAPELHREISSIIASKYSRIVDADRELTVYPGASTAIYIAIQ
                     AIINPGDEVVIFEPSYECYVPTIEICSGIIRPVRLVPPEFQPDWEEVERLVSDRTRLV
                     IINNPHNPSASVWRQEDFRKLEKILEGRDCLILSDEVYEFINFTEEGHLSIHSMEGLR
                     NKTLVISSFGKSLHVTGWRTGYCVAPPDVTIAMRKINQFSTFCANHAMQVGIASYLRD
                     YFDASRIRDTYRAKRDMMVNGIIDSRWKLLPCRGTYFMLLDYSSISDLPSMEFSEWLV
                     THKGIAVIPCSPFYSEGYDPRIIRICFAKKEETLIKAIDLLCNI"
     gene            5073..6491
                     /gene="dnaA"
                     /locus_tag="PLG24_06030"
     CDS             5073..6491
                     /gene="dnaA"
                     /locus_tag="PLG24_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HMZ24482.1"
                     /translation="MPELMSVNHNTVWKNCLNLIQENISEQGFKTWFKPIVPIRFEDQ
                     VLTIQVPNQYIYEMLEDQFIGILKKAIKSTIGPEGKLEYEIPVNKMPEASNTSSAETV
                     GLQDMDSARIKNPFVIPGIKKAAFDSELNPKFIFDNLIEGECNKMARSAGLAIAERPG
                     ITSFNPLFLYGGTGLGKTHLAHAIGNKIVEKFPSKRVKYISCEKLTNQVVYAFKTGTI
                     SDLSLLYNSFDVLIVDDIQFLSEKPRTQETFFFIFNELHQNQKQIVITSDRPPKDMEK
                     MEKRLISRFKWGLTAEMELPDYETRIAIAESKIEAEGFKVPEDVLEFICYNMQSNVRE
                     LEGMLISLAFASSLNNREINIQLAKEIINKFVDQLSKEVTIENIQSLVAEYFKMPIEK
                     LSGASRKRSIVIARQLSMYLAKNFTNKSLKTIGENFGGKDHTTVIYSCKAVQDMIDTD
                     PEFKGTVEELERKVRTSLAVSH"
     gene            6706..>7606
                     /locus_tag="PLG24_06035"
     CDS             6706..>7606
                     /locus_tag="PLG24_06035"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HMZ24483.1"
                     /translation="MKQLYMVVLLIWFQKSGAQNLYFPPITGNQWATVTPSELGWCDH
                     EIQPMYDYLEATHTKGFMVLKDGKIVLEKYFGSFNQDSVWYWASAGKSLTAFVIGVAQ
                     QKGLLSIEDKVSKYLGEGWTSCTRDQEDKIRIRHLLTMTSGLDDLVPDLFCNAPECLK
                     YKADAGTRWAYHTGVYSLLLDIVEKITGKNINNFLNQNIAWQIGMKGLWTSSSYGELY
                     FTNIRSMARYGLLILNKGVWDGKDLMKDSEYFNAMINTSQQLNPSYGYLWWLNGKSGY
                     LLPSLQITFPGSVIPNAPDDMVCA"
BASE COUNT         2332 a         1648 c         1514 g         2112 t
ORIGIN      
        1 atcccaaagg cgtttcaacc cggccaggtg agggctcata gctatctgat cattgagctg
       61 tatcataaca ttatcctgat agcctattga tggccttgca ttgtgataag tcgataactg
      121 atccaatggt atgattgtat tgatgccgtc attcccccct ttcagacgaa tcataatcag
      181 gatgcgatct gacttctcca gaattggtgg catggaccat cgcgtcagag tagatgcact
      241 cacgggcaat cctccaagca taaaacccaa tccgcctgca agaccgaatg atttgagaaa
      301 tctccgtctg gaatattcaa catgatctgc ctcatgcaat acaccatctt caagctttaa
      361 gcctcttttt tctatatcct tgttattcat tttatttaat tttgatgata tggaggttaa
      421 tctaacaaag cgtgacgtcc ggcaaacggg ccatctcatt aagaagcaga ttggtctgcc
      481 aacaaatgaa atccttttcc cagtcaagat tccataatcc ttgctcgtaa taattctcag
      541 gcaggtcggc tttgaatgca ataagcagac gatcataatc tgcctgggac gccaaccctg
      601 aaacaaaaaa ctgatcgaca actgtcctta cgacaaactc cacatcattg ctgttattgg
      661 ttagattttt cagataactg actacaaaat cctgattctg ccatatcagc cccccataca
      721 tccactctga ataaagccat cgtaccagaa ggctacctga gtcaatccat gacttgtcct
      781 ctttccaacc ggcaacatct accgggtcaa aataagtttg gcctaaggat gaactatacc
      841 aaatcatggc attgccctcc tcataattca aagtgtaatt aaactgcttt attatgcctg
      901 taaaatattc aatgtggctt ttgatatggg tattgaattc tgtagcgtca aagaaatgct
      961 cagagcagaa aagtgccttg aataccggaa gcaattcaaa atcattcgcc agcatgatat
     1021 cggctagttg ctgaatgatg ttatcattac cttctgcatt gacaaagtgg cgataaatct
     1081 ttccacaaat atgcctgcag atcaagtcgc ctttcttctc aaacagcaaa tcgatgacgc
     1141 cattgtagtc aaaattggca gtctcaccga agatggtctt aagtccatta tcatgggtaa
     1201 agggtgtaaa gtaaataata taattttcca gatctgcacc attataaccg gtaaatgccc
     1261 gtgccgtctc cttgatatca ttttctgtat aaccgttgtc aagccccagg gtaaacaatt
     1321 caaaaagctc tctagcatag ttttcattgg gttgccattt gttattctga aaaccgttca
     1381 ggaagataag catactgtgc tcaagcccca tttcccggat aaaggtcctg taattaccaa
     1441 gtgcattgcg ttgcagattg gtgacatatt gatacaggaa tggtgcgtaa taataatctt
     1501 caaacttagt taccagaaca ttgctccaaa acagggcaat cttatcacgc aatggtctct
     1561 tcaacttttc attgatccag tctgtgacaa attgggtata gatgatataa tgttcggtac
     1621 cgtcttcttc attgaattga cccggtttcc aatctgccca atcaggtttc ggaaaaacag
     1681 gcagattgaa agcttcatcc accaagcttt cgatgtattc caccggattc ttagagagga
     1741 tgtcaggaat ctctgctgcc gtaataccgt aacctatcct ggcatacaga tgcttaacac
     1801 gctgttcgtt ccagggcttt tccgcagtag ggacataggg actcaggggt gctaggttac
     1861 aatccgccat aacgaatgat taaggatgaa aaaatatgga aatgcttaat acgctgctaa
     1921 gataataaac gtccaaataa taaagaaaat atatttcctt caaaaaatat tcttacatcc
     1981 atcctaatga aaaccatgac agagaaaaag aatatcaaac aaaatgcctc aaaggaaaag
     2041 actatccgaa attttattcc aaagatggaa ttcaatgttc atagttctaa tttgattgaa
     2101 agaggatata atcatccggt aatatctgat tccgaagtat acctcaggtc atgatttcag
     2161 agtttcactg aatcaaacac tttaagtgca gcgctatttt gcaaacctta cggtcattat
     2221 ttgtcctatt ttgtgattca aaagataatc aacgccgcga ctcacccatc tcacgcaaag
     2281 aaacatttat tcacgatgtg taagcaaagt ttttcctacc ggctgataaa gggaagtcat
     2341 ggaacgatta gagccgccag agaaaataat aacattgatt cttaatcgtc cagtatgtct
     2401 gaccggatag catagtagca tagcaaggaa aaacttccct actccgactt catcctttcc
     2461 tcatccaaca aatgaaacat agctctgtac cgacttatct ccgacggatc aagacgggca
     2521 tgggagactc ctgcctttgt caatggtccg gtaacataat taccataagg tccgacaatc
     2581 gctgaatgcc cgttgtgagc ctttcctgta ccatcacttc cgaggcggtt gacaccgatg
     2641 acaaatgcct gattttctat tgcccgggct tgtaacagaa catcccaatg gatgatgcgt
     2701 gactccggcc aattggcgac aaaaaccatc aaatcagcct cctgcctatt gtaacaccat
     2761 aatggaaagc gcaggtcata acaaataagt ggctttacct tccagccttt gacctcaaag
     2821 gttacctgac tattacccga ggtgtatatt ttttcctcac ctgcatatgt gaagcaattc
     2881 cgtttatcat actgagctat gatttctcct tcggaagcta tgtaacaacg gttgtaatag
     2941 cgaccttcct cccggatggc aactgaaccc atgatggttg tctctaattc tcctgatcgg
     3001 atacataacc atgatacagc ctgtccatca tctttttgag ccactgttgc cgcatccata
     3061 ctgaatcctg tgttgaacat ttcagggagt atgatcaggt cactgcctcg tgctgtctcc
     3121 agcaatgtat caagatattc gagattggca ttactgtctt cccagatgat gtcgtattgc
     3181 actaaactga cattcagatg ttgcataata agtcgattgc tttaattaat gtttcctctt
     3241 ttttggcaaa acaaatcctg ataattcggg gatcataccc ctcagaataa aacgggctac
     3301 atggaatgac agcgatgccc ttatgggtaa cgagccactc agaaaattcc attgaaggca
     3361 agtctgaaat agaactataa tccagcaaca taaagtatgt acccctgcat ggcagaagtt
     3421 tccatcggct gtcaattatt ccattcacca tcatatccct cttggctctg tatgtatccc
     3481 tgatccggga tgcatcgaag taatcgcgca gataagaagc gatacctacc tgcatggcat
     3541 ggttggcaca aaaggtagag aattgattga ttttacgcat ggcaatagtg acatcgggag
     3601 gagcaacaca atagcctgta cgccaaccgg tcacatgaag agacttgcca aaactggaga
     3661 tcacaagcgt cttgttccgg agaccctcca tggaatgtat ggagagatgt ccctcctctg
     3721 taaaattgat aaactcatag acctcatctg acaggatcag acaatccctt ccctcaagaa
     3781 ttttttcaag cttcctgaaa tcttcttgtc gccacactga ggcagaaggg ttatgaggat
     3841 tgttgatgat aacgagcctg gtcctatcgg acacaagacg ctccacttcc tcccaatccg
     3901 gttgaaactc aggcggtaca agccggacag gccggatgat accactgcaa atctcgatag
     3961 tagggacata acactcatag gatggttcaa agattaccac ctcatcaccc ggattgatga
     4021 tggcctggat ggcaatgtaa attgcagtgg atgcgccggg atagactgtc agttctctat
     4081 ctgcatctac aatcctactg tatttactgg caatgattga cgagatttca cgatgaagtt
     4141 ccggagctcc ggaaggtctg gcatattgat tgtatcctga cttcatggcc tcagtgacag
     4201 catctatgag acgctcgtca accggaaaat cggggaaacc ttgtgcaaga ttgatggcac
     4261 catgttgtat ggccttggct gtcatcgtac taaatatgga tgcaccgtat tttgctagtt
     4321 tatctgtcat atatttatat cgcaagtaag tcgatggcgg atttttgatt gcatattaag
     4381 aacaggcaaa ataagcattt tggtaagatt gtaagaaatt cagaaggaga aaatggcatt
     4441 cttcaaggcg aaaatctatg gttatagtct gaaaaccggc ataggatggg ggccaaatgg
     4501 aagtaggtag atgagtaata cccggtaaac ccgttctgat caagtgtctg gaaatgaccg
     4561 taacttcata tcagactttt agccaaaaat attcttaaaa atatcccaaa agccgctaaa
     4621 gaagcgcatt tgtaagtatt attttcctac ctaattcacg ttggcccaac aaatttgtta
     4681 atccatcacc tcatccggcc caatccggca acctaatcgg tttcgtaccg gataatattt
     4741 aaacttcctc caaataatcc cataccaatg ctaccaattt taaatttccc ctaaaatatt
     4801 atatttatta tcatcaatca tttcctattt ttgaccgtcg gaaaacaaaa attcaacacc
     4861 aacctttaaa tttaaaaata atatatattt aatatttttg tatgtaaatt ttattggaga
     4921 aataatccgt ttttttgttt gtcaaacgtc aaagtttgtt gatatttgtc ctccgtccac
     4981 gccaaaatcc gacctttaaa ttaatactgt aatacttgca tcagggcttt acttgctgca
     5041 agaatattga taatgagcaa attatatagt aagtgcccga gttaatgtct gtgaatcaca
     5101 atacggtttg gaaaaattgc ttgaacctta ttcaggagaa catttctgaa caaggattta
     5161 aaacctggtt taagcctata gttccaatcc gatttgagga tcaggtgttg actatacaag
     5221 tgcccaatca atatatctat gaaatgcttg aggatcaatt cattggcatc ctcaaaaagg
     5281 caattaaatc aaccataggc ccggaaggaa agctcgaata tgaaattcct gtcaacaaga
     5341 tgcctgaggc ctcaaatact tcatcggctg agacagtagg tttacaggat atggactccg
     5401 ccaggatcaa aaaccccttt gtcattcccg gcatcaaaaa ggcagctttc gacagtgaat
     5461 tgaatccgaa atttattttt gacaatctga tagaaggaga atgtaataag atggcgcgct
     5521 ccgcaggtct ggccatcgcc gaacggccgg gcatcacatc cttcaatcca ctctttttgt
     5581 acgggggcac cggtctggga aaaacacacc tggctcatgc catcggcaac aaaatcgtag
     5641 agaaattccc atccaaacga gtaaaatata tctcctgcga aaagcttacc aatcaagtgg
     5701 tctacgcttt caaaacaggg acaatttcag acctatcgct tttgtataat tcgtttgatg
     5761 tgctgatcgt ggatgatatc cagttcctga gcgaaaaacc acggacccag gaaacctttt
     5821 tcttcatctt caacgaatta catcagaatc agaaacagat cgtaatcact tctgacaggc
     5881 cacccaagga tatggaaaaa atggagaagc ggttgatttc ccgcttcaaa tggggcctga
     5941 ctgccgaaat ggagctacct gattatgaaa cccggatagc aatcgctgaa tcaaaaattg
     6001 aggcagaagg atttaaggtg cctgaagacg tattggaatt tatctgctac aacatgcagt
     6061 ccaatgtcag ggagctggaa gggatgctca tttcgcttgc attcgcctcc agccttaata
     6121 acagggagat aaatattcaa ttggccaaag aaatcatcaa taaatttgtc gatcagctga
     6181 gtaaggaagt caccattgaa aatatccagt ccttagtcgc tgaatatttc aagatgccta
     6241 tagaaaaact ctccggggct agccgaaagc gttccattgt catcgccagg caattgtcga
     6301 tgtatctcgc taaaaacttt accaacaaat ccttgaaaac catcggagaa aactttggcg
     6361 gtaaggacca caccactgta atctattcct gcaaggcagt acaggatatg attgataccg
     6421 atcctgaatt caagggtacc gttgaggaac tcgaaagaaa ggtcaggaca agcctggcgg
     6481 taagccattg agtagccact ccgttttcat cactctttat taagatatgt ataatatgta
     6541 tattattgat atacaattaa aaaaattttt aatagtcgat gaaaaaaaag taaatttgcc
     6601 gaattccatg tgtaaaaaaa ggattaacca tttcacatag caaaaaaaat ctacctgacg
     6661 ataaaccaat tattgataaa tgtgtcaaag taaaaagata aagctatgaa gcaactttat
     6721 atggtcgtct tgctgatttg gttccagaaa tcgggtgccc agaatcttta ttttcccccg
     6781 atcaccggca atcagtgggc gactgtcacc ccaagtgaac ttggttggtg cgatcatgag
     6841 atacaaccaa tgtatgatta tctggaagca acgcatacca aaggcttcat ggtgttgaaa
     6901 gatggaaaga tcgtattgga aaagtatttt ggaagtttca atcaggattc cgtctggtat
     6961 tgggcatcgg ccggcaaatc acttacagcc tttgtaatag gagttgcaca acaaaaagga
     7021 ctactatcta ttgaagataa ggtatcgaag tatttgggtg agggatggac ctcatgcaca
     7081 cgcgatcagg aggataaaat acggatacga catttactga ctatgacatc gggactggat
     7141 gatctggttc cggatttgtt ctgcaatgcg cccgaatgtc tgaaatacaa ggcagacgca
     7201 ggcaccaggt gggcatatca tacaggagta tatagcttgc tgctggatat cgtcgaaaaa
     7261 attacaggaa aaaacatcaa caattttctc aaccagaata tagcctggca gattggcatg
     7321 aaaggtctat ggaccagttc ttcctatggc gaattgtatt tcaccaatat ccgctccatg
     7381 gctcgatacg gattattgat cctcaataaa ggtgtgtggg acggtaaaga tctgatgaaa
     7441 gacagtgagt atttcaatgc catgataaat acttcccagc aactgaatcc gtcttatgga
     7501 tatctatggt ggttgaatgg caaatcagga tatttgttgc cttctctgca aattactttc
     7561 ccgggttctg tgatacccaa tgctccggac gatatggtat gcgcat
//
LOCUS       DAOQQN010000123         7550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3888_length_7550_cov_4.344096, whole genome shotgun sequence.
ACCESSION   DAOQQN010000123 DAOQQN010000000
VERSION     DAOQQN010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3888_length_7550_cov_4.344096"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(850..1035)
                     /locus_tag="PLG24_06040"
     CDS             complement(850..1035)
                     /locus_tag="PLG24_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24484.1"
                     /translation="MKLIAIIIVTILFVLSVLNFLFRMPTLNITNIELAQVLVIGFFG
                     GALIALILNRVSDSKYF"
     gene            complement(1042..1413)
                     /locus_tag="PLG24_06045"
     CDS             complement(1042..1413)
                     /locus_tag="PLG24_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HMZ24485.1"
                     /translation="MNKGIITFVLLVLSNILMTFAWYGHLKFKDIAWLNKFGLPGVII
                     ISWLIAFFEYCFMIPANKIGFVENGGPFNLWQLKVMQEVITLIIFTVFTVFLFKNEVF
                     RLNHIIGFTFLILAVFFIFKD"
     gene            complement(1435..1968)
                     /locus_tag="PLG24_06050"
     CDS             complement(1435..1968)
                     /locus_tag="PLG24_06050"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HMZ24486.1"
                     /translation="MIDYIVFLRGINVNGQNLIKMEDLRKILIDIGLHEPKTYIQSGN
                     IWVKSELSEAESVESIIRKAIRERLGFDVPTLALPQDTLRRIIDNNPFTHEETDKKRL
                     YYTLLAEPPDRVRAEALMEMDTCGDKIVLGNKVVYLLVRDKYSKTKLTNQYIEKILKV
                     KATTRNHATMVKMTETI"
     gene            complement(1969..2532)
                     /gene="pth"
                     /locus_tag="PLG24_06055"
     CDS             complement(1969..2532)
                     /gene="pth"
                     /locus_tag="PLG24_06055"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HMZ24487.1"
                     /translation="MKYLVAGLGNMGAEYDGTRHNIGFDVADKLVSDAGEKFKHEHLG
                     DLSSIKYKGRTFYILKPSTYMNLSGKALQYWMRKLSIPQDRVIVILDDLNIPFKSIRI
                     RPNGADGGHNGLKNINEVLGNQNYARIRIGIGGDFKRGRQVNFVLGKWNKEEEQDLPE
                     IIAHAAKAVLAFGTLGITETMNQYTRK"
     gene            2822..5446
                     /gene="topA"
                     /locus_tag="PLG24_06060"
     CDS             2822..5446
                     /gene="topA"
                     /locus_tag="PLG24_06060"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HMZ24488.1"
                     /translation="MGQKLLIVESPAKAKTIEKYLGKDFVVKSSFGHVRDLEKGNNAI
                     DVDNNFTPHYVITPDKVKVVKDLKDISKKSEEVWLATDEDREGEAISWHLCEVLGLNP
                     HTTKRIVFREITKPAIQNAVANPRNLDLDLVNAQQARRVLDRLVGFELSEVLWRKIKA
                     SLSAGRVQSVAVRLVVDREREINNFEVETFFRVKASFPVMTPSEKTVMLVAESDQKFK
                     YEKDAYAFLEKCIGTSYHVQDITVKPGTRKPAPPFTTSTLQQEASRKLGFSVKRTMIA
                     AQKLYESGYITYMRTDSTNLSETAIADIAQNIELNFGSNYVQTRRYKTKNDSAQEAHE
                     AIRPSYIANKTVQVHDRDQERLYELIWKRTIASQMANAILEKTEVDVAIAAHPGHLLK
                     AVGEVVKFDGFLRVYFESVDDEDDETLNTLLPPVTKGQPLTLEEMTATQRFSKPPSRY
                     TEASLVKKMEELGIGRPSTYAPTISKIMEENRGYVVKESRDGMKRDFQVLTLRDDKIK
                     EATKSENTGTAKNQLFPTDMGMLVTDFLMEHFDKIMDYHFTAEIEKEFDEIAEGDLEW
                     VKMIRAFYNPFHKTVEQTRDTAARATGERMLGVDAVTGRSVLVRLSRYGKPIVQIGKA
                     EELAEGEKPQYANLKPGQSLETIEFDEAMSLFALPKDLGEYEGESVSVNIGRFGPYVK
                     VGDSFVSLPKGEDPFTVDMERAVEIILQKRLADAPVASYEGKPVTKGSGRFGPFIKWN
                     DLYINVPRRYNFNSLTQDDINELIRTKLEKESNRYIRLWESEKIAIENGRWGPFIRFG
                     KKMLKLGKNQEGKAYTSEQLSEITLDEVKAMIEAQEPGAFDKKTKSAASKTGAKAKVT
                     APKSGKTSKRASSAGTKK"
     gene            5451..6182
                     /locus_tag="PLG24_06065"
     CDS             5451..6182
                     /locus_tag="PLG24_06065"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HMZ24489.1"
                     /translation="MQAIILAAGLGTRLKPLTDDRPKALVLLKGMTLLDRAIAYLTVQ
                     GIERIVVNIHHFGNQIADHISHGQYDVPVLISDERRLLKDTGGALVQALPLLSPDEDI
                     LIYNTDIVTDMLLSKLFSAHIASGNDATLLTSVRESTRQLAFSPKGWLTGWQNKKNDE
                     IKGVLEPDSRMFSFSGIHIIKPSLIGYFGQEFGDAPFSVIPAYILAALDHKIGYYMTP
                     ESIKWMDVGSPEKLKLAEEYLLKEE"
     gene            complement(6432..>7550)
                     /locus_tag="PLG24_06070"
     CDS             complement(6432..>7550)
                     /locus_tag="PLG24_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ24490.1"
                     /translation="LLQGLVDTCAGRIVLNFTPYNGWSGSIENTVLMSKNGGPEIIVG
                     NPTTTNFILDTLDDDSEYCFRIRAEESITGNQSFSNSICLHSKKSKTITTLCVDDFAD
                     SGTSNIYDVVLTTNEDIPITGLFLQQADTPEGLADAPLTQLQITPGNQTQISPDNNRL
                     SYYRFVSIDACNKRIYSDIFSNLLLRATLKPGNEVDLYWNLPEWEGGSIREFRLERIS
                     TDGSTEIIATGDGNMTEYTHWLIDGKDGDLKYCYRIFSDITFDCGGSMYQITLPSNIS
                     CVEKTAGAFMANAFIVGGTFPEFMPIFYFKESIADYEMVIFDRYGGRIFSTKNPDQGW
                     DGSKNGQDLPNGVYSYYVRIQSGNGSLQELKGSITLLR"
BASE COUNT         2159 a         1559 c         1672 g         2160 t
ORIGIN      
        1 caacaccacc ggatatgcgc cccttacctt tggtttgcat catattgaaa ggcatttggg
       61 cgtgtattgt gcaactcaat gccaacagga agagtatgag aaaatttcgc atagtattgt
      121 ttttttatga taacacaaag gaaagggatt gagaatgaat ggaaggcctt ttttcgatta
      181 ataaaggcaa aaaaaagacg aagtagctct aaaggcgaat cctgttcaat ggaggaaaag
      241 gtatttccgt tgaaatccaa aaatcttgcc ggatgttatg tggtttgata tcgaaggccc
      301 ttgttatctt attgaagcca ccaggctatg cagttagccg ttgatcaggt aatcaatatt
      361 gcgacgatga ctattggttg ctgtatcagc ctgtcatgaa atataatttg cctgaaatcc
      421 ttagtggcat atccagcggt agttgattca gaatagagga agcattttga atcagtaatt
      481 aatcatgccg ttattatgag ccatccaaag aaatatgcat gaattcagga tgaatgggaa
      541 tgtaaatggt taaaatttgt gatgaatata aagttataac ttcagtatct tatctctacc
      601 taagatgccc tttgtaaatg gatccttgtc ctcgtatagc ttgtgttcag ttaaggtttc
      661 ggttttatag ccgaccaaat tattctataa atattgattt gttcttcttt ctaaatgttg
      721 aaacccgggt taaatatttc ttctaataat taattgcatg gtatcgttgt ttttaccttc
      781 gtttgatttt tactggatgt aaaaacgaat atcctgccag gcaagaaata aagccttttt
      841 ctttgcaatt cagaaatatt tcgaatcact tactctgttg agtatcaagg cgatcaacgc
      901 cccgccaaaa aagccgatta ctaaaacctg tgccaattct atattcgtaa tattgagcgt
      961 cggcatcctg aaaagaaaat tcaggactga caaaacaaac aggatcgtca caataatgat
     1021 ggctattaat ttcatccgga atcaatcttt aaatatgaaa aatacagcta aaatcagaaa
     1081 ggtaaaacca attatatgat taagccggaa tacttcattc ttgaataaaa aaacagtaaa
     1141 aacagtaaaa ataatcaatg tgatgacttc ctgcattacc ttcagttgcc aaagattgaa
     1201 gggtccgcca ttttccacaa agccaatctt attggccggt atcatgaagc aatattcgaa
     1261 aaatgctatg agccagctaa tgatgatgac tccaggcagc ccgaacttgt tgagccaggc
     1321 aatatccttg aatttaagat gcccgtacca ggcaaatgtc atcaggatat tggacagaac
     1381 aagcaataca aatgtaatga tccctttatt catttggttt tcgtctgaat gtaattatat
     1441 tgtttcggtc atctttacca ttgttgcatg atttctggtt gtggccttaa ctttaagtat
     1501 tttttcgata tattgattcg tcagcttcgt tttgctgtac ttatccctta ccagaagata
     1561 aactaccttg ttgcctaaca caatcttatc accgcaggta tccatttcca tcagtgcttc
     1621 agcgcggact ctatcaggag gttctgcaag caaagtgtaa tagagtcttt ttttgtccgt
     1681 ttcttcatgg gtaaatggat tattgtcaat gattcttcgc agggtgtctt gtggtaatgc
     1741 caatgttggg acatcgaagc ctaatcgttc ccggatggct ttacgtatga tggactccac
     1801 tgattcagcc tcagagagct cggattttac ccatatattt ccactttgga tataggtttt
     1861 tggttcatgc aaacctatgt caatgagtat tttcctcaag tcttccatct taatcaggtt
     1921 ttgaccattg acattgatcc cgcgcagaaa tacaatataa tctatcattt atttccgggt
     1981 atattgattc atcgtttctg tgatgcctaa ggtgccgaac gcaagtacag cttttgcggc
     2041 atgtgcgatg atttccggca agtcttgctc ctcctcttta ttccatttcc caagtacaaa
     2101 atttacttgt cttcctcttt taaaatcccc gcctatcccg attcggattc tggcgtagtt
     2161 ttgattaccc aatacttcat tgatgttttt taggccatta tggcctccat ctgcgccatt
     2221 gggcctgatg cggatgcttt taaatggaat attcaggtca tcgagtatga caatgaccct
     2281 gtcttgtgga atggacaatt tgcgcatcca gtattgtagt gccttcccgc tcagattcat
     2341 gtaggtcgaa ggtttcagga tatagaaggt cctgcccttg tattttatgg aagacaagtc
     2401 tccaagatgc tcgtgtttga atttttctcc tgcgtcactg actaatttat ctgcgacatc
     2461 aaacccgata ttatgtctgg tgccatcgta ttcggcaccc atattgccca agccggcgac
     2521 taaatatttc atctcgtcgt tgttgacctc ccgtttttta aaaaaattga aaattcccat
     2581 ttgatgcgac aaaaataggt tttagtgcat taaatgtaaa aggtggatta aaggtttttt
     2641 gctatttaca aatatcttgt tttatgtttg cggtgatttt cgtgtatttg atggggcatt
     2701 tttcaaatat acatgaggat tttttttctc ttggatttgt taaacattca ttgaatatgc
     2761 aactgtaaca gcctcacccg ggtgatgtac agtcctgttt tatctaaatg acacaatgaa
     2821 tatgggtcaa aaattattga ttgtcgagtc gcctgccaag gctaaaacga ttgaaaagta
     2881 tctgggaaag gatttcgtag tcaagtcaag tttcggccat gtgcgcgatc tggagaaagg
     2941 taataatgcc atcgatgtgg acaacaattt cacgccacat tatgtcatta ctcctgataa
     3001 ggtcaaagtc gtaaaggact tgaaagatat cagcaaaaag tcggaggagg tgtggctggc
     3061 aacggatgaa gaccgcgaag gagaagccat tagctggcat ctgtgtgaag tgttggggct
     3121 caacccccat accaccaagc gaattgtttt ccgcgaaatt accaagcccg ccattcagaa
     3181 tgcagtcgcc aacccacgca acctcgatct ggatctggtc aatgcacaac aagcgcgccg
     3241 ggtactggac agactcgttg ggtttgagct ttcagaggtt ctttggagga agatcaaggc
     3301 ctcactatct gcaggccgtg tccaatcggt tgccgtgcgt cttgtggtgg atcgtgaacg
     3361 ggagatcaac aacttcgagg tagagacttt cttccgagtc aaggcatcct tcccggtgat
     3421 gacaccctca gaaaagacgg ttatgctggt cgctgaatcc gatcaaaaat tcaaatatga
     3481 aaaggatgct tacgcttttt tggaaaaatg catcggcaca tcctaccatg tacaagatat
     3541 aaccgtcaag ccaggcacac gcaaaccggc gccgcctttt actacatcga ctcttcagca
     3601 ggaagccagt cgcaagctgg gcttctctgt caaaagaacc atgattgctg cccaaaagct
     3661 ttacgaatcc ggttatatca cctatatgcg taccgactcg accaatctga gtgagacagc
     3721 tattgcagat atcgcccaga atattgaact taacttcgga agtaattatg tccaaacccg
     3781 cagatataag acaaagaacg attcggctca agaagcacat gaagccatca ggccttccta
     3841 tatcgccaat aagactgttc aggtgcatga ccgggatcag gaacgtttgt atgaactgat
     3901 ctggaaacgt acaatcgctt ctcagatggc caatgctatt ctggaaaaaa ctgaagtaga
     3961 tgtggccatt gccgcacatc cgggtcatct gctcaaagct gtaggtgaag tggtcaagtt
     4021 cgatggcttc cttcgcgtat attttgaatc ggtcgatgat gaggatgatg aaaccctgaa
     4081 taccttatta cctcctgtta ccaaaggtca acccttgaca cttgaagaga tgacggcaac
     4141 acaacgattc tccaagccgc cttcaagata taccgaggcc agcctggtca agaagatgga
     4201 agaactcggc atcggtcgac cttccaccta tgcaccgact atttctaaaa ttatggaaga
     4261 gaatcgcggc tatgtagtca aggagtctcg cgatggtatg aaacgagact ttcaggtact
     4321 taccctgaga gatgataaaa tcaaggaagc aacgaagtca gaaaacacag gcactgccaa
     4381 aaatcagctt tttccgacgg atatgggtat gctggtgaca gattttctga tggaacattt
     4441 tgataagatt atggattatc atttcacagc cgagatcgag aaggaatttg acgagatcgc
     4501 tgaaggtgac ctcgaatggg tcaagatgat ccgtgccttt tataatccat ttcacaagac
     4561 tgtagagcag acaagggata cagcagcaag agctaccggg gagagaatgc tgggcgtgga
     4621 tgcggtcacc ggtcgttctg tactggttcg cctaagccgg tacggaaaac caatcgtcca
     4681 gatcggtaag gcggaagaac ttgcggaagg agagaagccg cagtacgcca accttaagcc
     4741 aggccagtcc ctcgaaacca ttgagttcga tgaggcgatg agcctgtttg cattacccaa
     4801 ggatttaggc gagtatgaag gcgaatcggt ttcggttaat attggtcgtt ttggccctta
     4861 tgtcaaggtg ggagacagct tcgtcagctt gccaaagggc gaggatccct ttacggtcga
     4921 catggaaaga gccgttgaga tcatcctgca aaaaaggttg gcagatgctc ccgtagccag
     4981 ctatgaaggt aagccagtaa ccaagggtag tggtcgtttc ggtcccttta tcaaatggaa
     5041 tgacttgtac atcaatgtcc cacgacgata caacttcaac agtcttactc aggatgatat
     5101 caatgaattg atccgtacaa agctggagaa agaatccaat cgttatatcc gcctgtggga
     5161 atcagaaaag atagccatag aaaacggtcg atggggacct ttcattcgtt ttggaaagaa
     5221 aatgctaaag ttgggtaaga atcaggaagg caaggcatat acatcagagc aattatcaga
     5281 aataacactg gacgaagtaa aagcaatgat agaggcacag gaacccggcg ccttcgataa
     5341 gaagactaaa tcagcagcct caaaaaccgg tgccaaagct aaggtaactg ctccgaagtc
     5401 cgggaagaca tcaaagaggg catcttccgc cggaacaaaa aagtaatacc atgcaggcaa
     5461 tcatcctcgc agctggactt ggcacccgac ttaaaccatt gacggatgac aggccgaagg
     5521 ctttggtgtt attaaaagga atgacactgc tggatagagc cattgcctac cttaccgttc
     5581 aggggattga acggattgtc gtcaatatcc atcattttgg aaatcagata gccgatcata
     5641 ttagccatgg gcaatatgat gttcctgttc tgatttcgga tgaaagacgg ctactgaaag
     5701 ataccggagg tgccttggta caggcattgc ccctgttgag tccggatgaa gatatcctga
     5761 tatacaatac ggatatcgtt accgatatgt tactgagtaa gcttttttct gcacatatag
     5821 catccggcaa tgatgccaca ctcttaacat cagttagaga gagcacacga caactggcat
     5881 tttcgccaaa aggctggctt acaggctggc aaaataagaa aaatgatgag ataaaaggcg
     5941 ttctcgaacc tgatagcagg atgttttcat tcagcggtat acacatcatc aagccgtcct
     6001 taattggata ctttggtcag gaattcggcg atgcaccatt ttctgtcatt ccggcatata
     6061 tactggcagc cttggatcac aaaatcggct attatatgac tcctgaaagt ataaagtgga
     6121 tggatgtcgg tagtcctgaa aaactgaaac tggccgaaga atatttgctg aaggaggaat
     6181 agtttttcta cccggtgtaa ttcggattta tagttatacc aagatgattt atataatagt
     6241 ccataaattt ttggtataat aatgaatgta ccttcggtat taatatggat tctttgcctt
     6301 cccgaactgt tgaaagtaag tgtgctctgt cagtaagttc ttttgtaaag gtgcaaggtt
     6361 tatgattcat ttgttgacat ccttactgtc tttcaataaa atagccaaca gatcgagaaa
     6421 gaaatttcct tctacctcaa taatgttatt gatcctttca attcttgcag gctgccattt
     6481 cctgactgaa ttcggacata ataggaatag acaccgttgg gcagatcctg accgtttttg
     6541 ctgccgtccc aaccctggtc aggattttta gtggagaaaa tcctgcctcc ataacggtcg
     6601 aagatgacca tttcatagtc ggcaatgctt tccttgaagt agaatatcgg cataaattcc
     6661 gggaaagtac ctccgacaat aaaggcattt gccatgaaag cccccgcagt tttctctaca
     6721 caggaaatgt tggatggtaa tgttatctga tacatgctac ctccacagtc gaatgttatg
     6781 tcggaaaaga tcctgtagca atatttcagg tccccgtcct tgccgtcaat aagccaatgg
     6841 gtgtactccg tcatattgcc gtcaccggtg gctatgattt cggtgctgcc atcggtagat
     6901 atgcgctcca accgaaattc ccggatgctt cctccttccc attcgggtag gttccagtat
     6961 agatccactt cattaccagg tttcagggtg gcacggagca ataagttgct gaagatgtct
     7021 gaatatattc gcttgttgca ggcatcgatg gatacgaagc gatagtatga caatcgattg
     7081 ttatccgggg agatctgtgt ttgattgccc ggagtaattt gaagttgagt caatggagcg
     7141 tctgccaatc cttccggtgt gtcagcttgt tgcaggaaaa gtcctgtgat tggaatgtct
     7201 tcattggttg tcagcacaac atcataaata ttgctcgtcc cgctatccgc aaaatcgtct
     7261 acacaaagtg ttgtgatagt cttgcttttc ttggaatgaa ggcaaattga attggaaaaa
     7321 ctttgattcc cggttattga ttcctctgct ctgatgcgga agcaatattc agagtcatca
     7381 tcaagtgtat caagtataaa gttcgttgtc gttggatttc cgacgatgat ttccggtccg
     7441 ccgttcttgg acatcaatac tgtattttca atcgagcccg accagccatt atagggtgta
     7501 aagttaagca ctattcttcc tgcacaggta tcaaccaggc cttggaggag
//
LOCUS       DAOQQN010000124         7547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3891_length_7547_cov_6.943673, whole genome shotgun sequence.
ACCESSION   DAOQQN010000124 DAOQQN010000000
VERSION     DAOQQN010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7547
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3891_length_7547_cov_6.943673"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PLG24_06075"
     CDS             complement(<1..588)
                     /locus_tag="PLG24_06075"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HMZ24491.1"
                     /translation="MTKNNTTQISKQDFLLGRFDPQKHPDFVLIDQKYADKEGMMMQG
                     EAYSAFLKMAHAGELAGHSFCILSATRNFFYQKYLWENKWYGIFPIQGNIDACTSFQD
                     EVYRARKIMEYSAMPGSSRHHWGTDIDINALDNDYFEQGEGLAWYRWMTENAGAYGFC
                     QPYTAGRKYGYKEEKWHWTYLPLSKQYTEEIRRTIT"
     gene            complement(795..1391)
                     /locus_tag="PLG24_06080"
     CDS             complement(795..1391)
                     /locus_tag="PLG24_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24492.1"
                     /translation="MTRFVCYNIVFLFLLSGMMTSCGKEDPINPDTPKEDTTFSFKCS
                     IEDIPWDGSKKALANPQIKLLFPKVDSLPQAFFSGDTLLIGVGGKYNNYDATLLMAIR
                     VANKADLKGTYNFAHKIGSLEVGKGIGVFDGSSTDLLTYATGLDLLEYVDDSKVIITE
                     HKNGRISGTFQFRMEHIISKQGFNAVKEGTFKNFKIKQ"
     gene            complement(1398..2825)
                     /locus_tag="PLG24_06085"
     CDS             complement(1398..2825)
                     /locus_tag="PLG24_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ24493.1"
                     /translation="MQKQVLEAEQKALEINLDPSIYGTFAEIGAGQEVARYFFQAGAA
                     AGTIAKTMSAYDKTYSDEIYGAEPSGRYVCESRLYKMLDHEFKLLVDRLGKIRPDTTF
                     FAYADTVSATNYARTIPGDGWLGIRFQKTPDSEPNDLVLHVKMLDKDNRQQQQAVGIL
                     GVNLIYACFFLSEDMDEMLKALIDGLENRVMIDMIRLTGPSFKHVDNRALSFKLVRHG
                     LSDVAMFDAKGRNIHPSEFLYKKNVLIVRGAFRPITWASYDIIRSSHRQFMADENLSK
                     DDVKVLTEITLDILSVHGEPSESDFLERATALNHVGMIVCITNCGNQQKLIKYMEDFK
                     VPRIGLSLTAGYTARYIDETIEKFRGGKLLEGLGGMFPGNVRVYVYPSRMPGTKEIMN
                     ARCIKIDPDMKYLYEHLLTTLQIVDIEQFKKAYLKISARSVLKMIQVDDSRWEKFVPK
                     KIISLVKTMKLFGYKAQTKTISESK"
     gene            3761..4723
                     /locus_tag="PLG24_06090"
     CDS             3761..4723
                     /locus_tag="PLG24_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24494.1"
                     /translation="MNKVFLGSALFAPVGGLIGIVAAKSLDDNSPLSTEGKTATTIAV
                     DKPEETIAVASAPAENAANIIPETESEEMHINFISDLPCGHPSDNLSFGEAFREQRNI
                     LGQGGVFEYQGKYYNTYYKEEWDAMTDEQKNEYFLSIDSKIDYSKSFITGHHKGNQYE
                     VSVQDFNQSAFSNHNSPFIPEDQAEGYVPFEYGHTPMEAGPNAIHLQPDFEVPSTVIV
                     DDYDFNSGSHSFNGEIDHSYFNYDNSIGLNDDLELQASIVGSDDSILDVEPGDLAHHF
                     DYNQYGNSDGAGHETIYFDHDMDTPDSLDENGHHEHGYTFPDDF"
     gene            4752..5258
                     /locus_tag="PLG24_06095"
     CDS             4752..5258
                     /locus_tag="PLG24_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HMZ24495.1"
                     /translation="MELKCPFCDERIILANIEKYVGKQIRVKCVNKSCQQYFVTRVPD
                     SSDKTIVFSAGSVKTRAKLTVLASKFAPFKEYYLGEGNYIVGRYSIDSIANIAILTTD
                     KLVSRQHCEITGRQNADGGIDFTIKDAGSKNFIYINNRQLESDEEIYLNEKDIIRLGR
                     IELQFHRD"
     gene            5268..5975
                     /locus_tag="PLG24_06100"
     CDS             5268..5975
                     /locus_tag="PLG24_06100"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HMZ24496.1"
                     /translation="MQIETFQHLGRREEQQDAYFISSDNKLFLVCDGAGGMENGALAS
                     NLAIKTISEAYSRQSGILDLADFKSMIYESIYKINSLLDKNVATTLVCLYIDNEYALY
                     CHLGDSKLFYFNEKNNSWIATKDHSIVQELFDAGIIKTEEEILNHPLRNRITKAITPG
                     IMPAPDDLEVEKIKNLNPGSIFVLCSDGVLEKTTNSAFAGVFNQQTSDLNEKVEIIRH
                     QCVQKSADNCTLIAIEI"
     gene            6010..>7547
                     /locus_tag="PLG24_06105"
     CDS             6010..>7547
                     /locus_tag="PLG24_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HMZ24497.1"
                     /translation="MRFIILSLSLFLFTNFVSAQKQVILYYDGNGIRSSKAECAYYKV
                     INYNKAGKIIGKIKDFYKSGEGYTTSDGAKEINRDNDNKSVYWGKQIRYYKSGAMKAI
                     RLFSLDGDIIYEKKWYSSGKIKSFGSYKTKYGSTFIEFAEDGSIIASYRNRKGEGDPR
                     VFVQKDNQYLIAFMEDFSTNQNRQFWEYEMPLEEGQFEIGVGYHIITEQNGHFAKILP
                     FSDSFDDSYMFSTNIGSIKGDKNTFHGIVYDYSGPDQFSCFGINGMGKFCIYHVNKSK
                     SFFEVPITDNSNINTGRNNNNLTIQYLDEKISFLINDVEVYTSNKNEFGEKYFGLFSD
                     GGRKDVVFQNISLLYLNEEIRTMMDDMIVGDGAFKTGTGFIIDDRGYMITNHHVIENA
                     DFIEVEFKWEGKTKTFPARIVSTDKEKDLSILRIISNDFKLPKKIPFAIEKADIPTGS
                     EVFTLGYPEAFNMGREVKFTDGKISALSGIKNNKSVYQTSIPARGGNSGGAVFGPNGN
                     IIGVL"
BASE COUNT         2285 a         1589 c         1507 g         2166 t
ORIGIN      
        1 ggtgatcgtt ctacgaatct cttctgtata ttgtttggaa agtggaagat atgtccagtg
       61 ccacttttct tccttgtagc cgtatttgcg acccgccgta tagggctggc aaaatccgta
      121 ggcaccggca ttttccgtca tccaccgata ccaggcaagt ccctctcctt gttcaaaata
      181 atcattgtcg agtgcgttga tgtcaatatc cgtaccccag tggtgtctgg aagaacccgg
      241 catggctgaa tattccatga tcttcctggc acgatagact tcatcctgaa aggatgtaca
      301 tgcatcgatg ttgccttgga tcggaaagat accataccac ttgttttccc agagatattt
      361 ctgataaaag aaattacggg ttgctgaaag gatacagaaa gagtgtccgg caagctcgcc
      421 ggcatgagcc atcttcagaa atgcgctgta agcctcgcct tgcatcatca tgccctcttt
      481 gtcggcatac ttctgatcta tcaaaacaaa atccggatgt ttctgcgggt caaatcgacc
      541 caacagaaag tcctgtttgc tgatctgtgt tgtattattc tttgtcattg aatcatcgga
      601 attacaaccc caaaaagcca atgatacaag caataccagt attttttgtc cctgcatcga
      661 tggtctttta gcctttatta tattctaacc aaagtatcaa gaaatatagt aactcaggaa
      721 actatccata gaataaacct ttgccccgga tgcgggtact gatgatcttt gatcatttct
      781 tttcgcccct ttgcttattg cttgattttg aaatttttaa atgtaccctc tttgaccgca
      841 ttaaaaccct gttttgaaat gatatgctcc atcctgaact ggaatgttcc tgaaatgcgc
      901 ccgtttttat gctcggtaat aataaccttc gagtcatcaa catactcaag taagtcaagt
      961 cctgtggcat aagtaagcaa atctgtcgaa ctcccatcaa atactccgat gccctttccg
     1021 acttcgaggc taccaatttt atgggcaaaa ttatatgtgc ctttcaggtc ggccttgttg
     1081 gccactcgaa tagccatcag taaagttgca tcatagttat tgtatttccc gccgacacca
     1141 atcagcaggg tgtcgccgga aaagaacgcc tgaggtaaag aatcgacctt agggaacagt
     1201 aacttaatct gcggattggc caatgctttc tttgaaccat cccagggaat atcctcaata
     1261 ctacatttaa atgaaaatgt agtatcctct ttcggtgtat cggggtttat cggatcttcc
     1321 tttccgcagg aagtcatcat acctgaaagt aaaaataaaa agacaatgtt ataacatacg
     1381 aatcttgtca tatgatgtta ttttgattca gaaatggttt ttgtttgagc tttatacccg
     1441 aagagcttca ttgttttgac cagagagatt attttctttg ggacgaactt ttcccaacgg
     1501 ctatcatcca cctggatcat ctttagcaca ctgcgagcgc tgatcttcag atatgctttt
     1561 ttgaattgct caatatcgac tatctggaga gtagtcagga ggtgttcgta tagatacttc
     1621 atgtccggat cgattttgat gcatcttgca ttcataattt cctttgttcc cggcatcctg
     1681 gatggataga catataccct tacattaccg ggaaacatgc cccccaatcc ttccaggagc
     1741 tttcctcccc ggaatttttc gatggtctcg tcaatatatc gtgccgtata tccggcagtc
     1801 aacgaaagcc cgatgcgtgg gaccttgaaa tcctccatat atttgatcaa cttttgctga
     1861 ttgccacagt tggtgatgca cacaatcatg ccaacatgat tgagcgctgt ggccctttcc
     1921 agaaaatcac tctcactcgg ctccccatga acactaagga tatccagggt gatttctgtc
     1981 aaaaccttga catcatcctt gctcagattc tcatctgcca taaattgtcg atgactactg
     2041 cggataatat cataactggc ccatgtaata ggcctgaatg cccctcttac gatcaataca
     2101 ttcttcttat acagaaattc cgatgggtgg atatttctcc ctttggcatc gaacatcgct
     2161 acgtccgaca acccatgtcg aaccaactta aagctaagag cacggttatc cacatgctta
     2221 aatgaagggc ccgtcaaccg gatcatatct atcatgaccc tgttttctaa accgtctatc
     2281 aatgccttga gcatttcgtc catatcttcg gaaaggaaga aacatgcgta tatcaggttg
     2341 acgccaagga taccgacggc ctgttgttgt tgacgattgt ctttgtcgag catcttgaca
     2401 tggagtacaa gatcattggg ctcactgtcc ggcgttttct ggaacctgat tcccagccac
     2461 ccgtcacccg ggatggttcg ggcataattg gtggcggaaa ccgtgtcggc ataggcaaaa
     2521 aatgttgtgt ccggccgtat ctttcccaac cgatcaacca gcaacttaaa ctcatggtcc
     2581 aacatcttgt acagtcggga ctcacataca taacgaccac tgggttctgc gccatatatt
     2641 tcatcggaat aggtcttgtc ataagcagac atggtcttgg cgatggtgcc tgcagcagca
     2701 ccagcctgaa aaaagtatct ggcgacctcc tgtcctgcgc ctatctcagc aaaggtgcca
     2761 taaatacttg gatcaagatt gatctcaagc gctttttgtt cggcttctaa tacttgtttc
     2821 tgcatcaata ttccgtaaag ataggtgcaa agatagtgat aattggttga agttctgcct
     2881 gatcctgtca cgcacgatat tcacaaaaaa taaggacatt agtaagacac gaaacccgtg
     2941 ttgttccctc cttgtactgc gcttactatt gaataggaca atctcgtcct ggacttattc
     3001 tcccggacaa tttgttacaa ccggtagtgt ggaataatcg atgaataacg accaaaatat
     3061 cgcgttttac aaagccttca gcattggttt taccaattag ataacaaatt ttactgagaa
     3121 acaaaaaacc aaagctatgt agaattatta atgtttcttc gacggtgaat tctacaaggc
     3181 cggtgcccca aagagcttac ttttggcgga tgccgggttc ttcatttgtg cattttgtta
     3241 ttattacgtt gaattctgat ctgacattct taattcatgc tgaagacaaa agatgggttc
     3301 cttaaattta tggtgttttg tcagacactg ataaaacacc ataaatatcg aacaaatcaa
     3361 tttattttac tgcatcacac attttgaaag ttatccgcat tccatcttta ttattcgaaa
     3421 agggcacttt gtgatttaca cccggttagc cgatatctgt aacaaaagac ttaggattca
     3481 aataaaaatt agcaatgccg tgtcttttcg ggccttcaat aaatttcaat ttgaataaat
     3541 accccggtca taatcttcaa aatcacggat tcatacaaat taagctttga ttcatacata
     3601 tttaaagcct ggattaggga tagaatatta attttaagcg agaattatta aagcattaaa
     3661 ccaatacagt atgtcattta caaaaacatc tacatcaaat ctttcaggca aattgtccgg
     3721 aaaatccatt ggaatgggat caaaaggaca accttggtat atgaataaag tttttctggg
     3781 gtcagccctg tttgctccgg tcggaggctt aatcggtata gtagctgcca agtccctgga
     3841 tgacaactcc ccattatcaa cagaaggaaa aacagcaaca accatcgcag tagataagcc
     3901 ggaagaaacc atcgcagttg cgagcgcccc ggctgagaat gcggcaaata ttatacccga
     3961 aaccgaatcg gaagaaatgc atatcaattt tatctccgat ctgccttgtg gacatccgtc
     4021 cgacaacctt tctttcggag aagcattccg tgaacaaaga aatattttgg gtcagggggg
     4081 cgtatttgaa tatcaaggca aatattataa cacctattac aaggaagaat gggatgctat
     4141 gacggatgaa cagaagaatg aatatttcct gtcaatagat tcaaagattg attattcaaa
     4201 gtctttcata acaggccatc acaaaggaaa tcaatatgag gtgtccgtac aggatttcaa
     4261 ccagagtgcc ttttctaatc ataattcacc attcattccc gaagaccagg ctgaaggata
     4321 tgtgcccttt gaatacggtc atacacccat ggaagccgga ccaaacgcca ttcatcttca
     4381 acctgatttt gaggttccct ccacagtaat agttgatgat tatgacttta attccggctc
     4441 acactcgttc aatggagaaa tagaccattc ctacttcaat tacgataatt cgataggatt
     4501 gaatgatgac ttagagcttc aggcttcgat cgtaggaagt gacgatagca tcctggatgt
     4561 agagcccggt gatttggcac accatttcga ttataatcaa tacggtaatt ctgatggcgc
     4621 cggtcatgaa acaatatatt tcgatcatga tatggatact ccggattcat tggatgaaaa
     4681 tggccatcat gaacatggat ataccttccc ggatgatttt tgatatacgg ataacctcgt
     4741 ggtattgaag tatggagttg aagtgtcctt tttgtgatga gagaattata cttgccaata
     4801 tcgaaaaata tgtagggaag caaatccggg tcaaatgtgt caataagtca tgccagcaat
     4861 actttgttac cagagttcct gattcatccg acaaaacaat tgttttttct gccggatctg
     4921 tcaagacgag ggcaaagctg accgttcttg ccagtaaatt cgcccctttc aaggaatatt
     4981 atctcggcga aggtaactat atcgtcggca gatacagtat tgactccatt gcaaatattg
     5041 ccatcctcac cactgataaa ttggtatcaa ggcaacattg tgaaatcact gggaggcaaa
     5101 atgccgatgg gggcattgat tttacaatta aggatgcggg aagtaaaaac ttcatctata
     5161 tcaacaaccg gcaactggaa tctgatgagg aaatctatct caatgaaaaa gatatcatca
     5221 gactgggaag aattgaactt caatttcatc gggattaaaa acgagctatg cagattgaaa
     5281 cttttcaaca tttaggaaga agagaagaac agcaagatgc ctactttatt tcttctgaca
     5341 ataagctttt tttggtatgt gatggtgccg gtggaatgga aaacggagcc cttgccagca
     5401 acctggctat aaaaacaatc tctgaggctt atagccgtca atccggtatc ctggatcttg
     5461 ctgatttcaa atccatgata tatgaaagta tctacaaaat aaacagcctc ctggacaaaa
     5521 atgtcgcaac aaccctggta tgcttgtata ttgacaatga atatgcctta tattgccatc
     5581 tcggcgacag taagttgttc tacttcaatg aaaagaataa ttcatggata gccaccaaag
     5641 atcattctat cgttcaagaa ctttttgatg caggaattat aaaaaccgaa gaagaaatcc
     5701 tcaaccaccc actcagaaac agaattacaa aggcgattac tcccggaatc atgcctgccc
     5761 ctgacgacct tgaggttgaa aaaatcaaaa atctgaaccc gggaagtatc tttgtactat
     5821 gttctgatgg tgtgttggaa aaaactacga atagcgcttt tgccggagta ttcaatcaac
     5881 agacgtcaga tttgaatgaa aaggttgaaa ttatcaggca tcaatgtgtc caaaaatcag
     5941 ccgataattg taccttgatc gcgatagaga tttagcaaaa acattatcca ttgacaataa
     6001 ccaaacctta tgagatttat tattctttcc ctttccttgt ttcttttcac caatttcgtt
     6061 tctgctcaaa agcaggttat tctttattat gacggcaatg gaatacgatc ctccaaagca
     6121 gaatgtgctt attataaagt gatcaactat aacaaagccg ggaaaattat cggtaagatt
     6181 aaggactttt ataagtcggg agagggatat accacctccg atggggccaa ggaaatcaac
     6241 cgggacaatg acaacaagtc cgtatattgg ggtaagcaaa tcaggtatta caagtctggc
     6301 gccatgaagg ctattcgcct gtttagcctt gatggtgata ttatttatga aaagaaatgg
     6361 tattccagtg gcaagatcaa gtcattcgga tcctataaaa ctaaatacgg atctactttc
     6421 atagagtttg cagaagacgg atccatcata gcaagttata gaaaccgcaa gggagaaggc
     6481 gatccgaggg tttttgtaca aaaagataat caatatctaa tcgcctttat ggaagatttc
     6541 tccaccaacc aaaaccggca attctgggaa tatgaaatgc ccttggaaga aggtcaattt
     6601 gaaattggag taggatacca cataataaca gaacaaaatg ggcattttgc caagatcctg
     6661 ccattttcgg attcctttga cgattcatac atgttcagta caaacatagg atctataaaa
     6721 ggcgacaaaa acacttttca cggcattgta tatgattatt ccggacccga tcaattttcc
     6781 tgctttggta tcaatggtat gggcaagttc tgcatctatc atgtcaataa gtccaaaagc
     6841 ttttttgaag tcccgataac agataacagc aacattaata ccggcaggaa taacaacaac
     6901 cttacaatcc aatatttgga tgaaaagatt tcatttttaa taaatgacgt tgaagtatat
     6961 acttccaaca aaaatgaatt cggggaaaaa tatttcggct tgttttcgga tggtggaaga
     7021 aaggatgtcg tgttccagaa tatatcatta ctctacctga acgaagaaat acggaccatg
     7081 atggacgaca tgatagtagg agatggagca tttaaaacag gcaccggctt tattatagat
     7141 gacagaggat atatgattac caatcatcac gtcattgaaa atgccgattt tatcgaggta
     7201 gaatttaaat gggaaggaaa gacaaaaacc tttcctgcca ggatcgtgag taccgacaag
     7261 gaaaaagacc tttctatttt gcgtatcatt tccaatgatt tcaaacttcc taaaaagatc
     7321 ccctttgcca tcgaaaaagc ggacatccct accggttctg aagtgtttac gcttggttat
     7381 cccgaagcat tcaatatggg tagggaagtg aagtttaccg atggcaagat tagtgccctg
     7441 tccggaatta aaaacaacaa gtctgtatac caaacttcga ttccggcccg gggcggaaat
     7501 tccggtggag cagtatttgg tcccaatgga aatattatag gcgtact
//
LOCUS       DAOQQN010000125         7511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3933_length_7511_cov_5.944608, whole genome shotgun sequence.
ACCESSION   DAOQQN010000125 DAOQQN010000000
VERSION     DAOQQN010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7511
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3933_length_7511_cov_5.944608"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PLG24_06110"
     CDS             complement(<1..379)
                     /locus_tag="PLG24_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24498.1"
                     /translation="MGGQYYRNIHKAIFLIFIFSGVLALFSDCRNNNSSFSGKSNKTV
                     EGKAIQNSVNEYRGRYLFSNNVDTKGEIIIMYNGTFTGQLTDIYDNVLDNLSGRLEGN
                     KLLFDGNNSNIMMTGTISGDMINC"
     gene            complement(441..740)
                     /locus_tag="PLG24_06115"
     CDS             complement(441..740)
                     /locus_tag="PLG24_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24499.1"
                     /translation="MENKLFKFYNRWSRPFFILITLFLLFSCENADSKVSQKENSTNE
                     QKNVEVSDNFSGLYTIRDSEISGALSFERRDSKDGVTSGDVVLLADFTNVGGKKL"
     gene            1722..1895
                     /locus_tag="PLG24_06120"
     CDS             1722..1895
                     /locus_tag="PLG24_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24500.1"
                     /translation="MKKNYLFIGISVLIIAFSINNLTLDIDNRGHYIGNGILCGIGIS
                     IFVTQMVRLIRNK"
     gene            complement(2021..3265)
                     /gene="ilvA"
                     /locus_tag="PLG24_06125"
     CDS             complement(2021..3265)
                     /gene="ilvA"
                     /locus_tag="PLG24_06125"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HMZ24501.1"
                     /translation="MNLFEKVVQAQEKIKGVAVKTPLIKNENFSDSLSAQIWLKREDL
                     QPIRSYKLRGAFNKIITLTPDQSARGVVCASAGNHAQGVAYACQKLGIYASIFMPVTT
                     PQQKLRQVRLFGKEYVDIILKGDTFDDSFQEAIQYCNRKNAIFIHPFDDEDVIAGQGT
                     VGMEIIDDAPQPIDYLFVPIGGGGLASGLITVFKKLSPQTKIIGVEPAGAASMKSAVE
                     RGQVIALPDIDKFVDGAAVSRVGDNTFGICQEGLSDIIPVPEGKVCSAILKLYNEEAI
                     IVEPAGALTIAALDLYKDEIKGKNVVCIISGGNNDITRTEEIKERSLLYEGLKHYFMV
                     NFPQRPGALKEFVNNVLGPNDDITYFQFSQKNNRENGPAVVGIELKRQDDFNMLKENL
                     NVHNINYQYLNENADLFTHLIF"
     gene            complement(3262..4308)
                     /gene="ilvC"
                     /locus_tag="PLG24_06130"
     CDS             complement(3262..4308)
                     /gene="ilvC"
                     /locus_tag="PLG24_06130"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HMZ24502.1"
                     /translation="MATLNFGGVQEEVVTREDFPLEKAKATLHDETMAVIGYGVQGPG
                     QALNLRDNGFRVIVGQRRHSASWDKALADGWKEGSTLFDIEEACARGSILMNLLSDAG
                     QIQAWDTMVPHLHAGKALYFSHGFGVTFHDKTGIKPPKDVDVILVAPKGSGTSLRSLF
                     LRGQGLNSSYAVYQDATGKAVERALAVGIGIGSGYLFETTFEKEVYSDLTGERGVLMG
                     AIAGIFEAQYNVLRQRGHSPSEAFNETVEELTQSLMPLVAENGMDWMFANCSTTAQRG
                     ALDWKGRFRDVTEPVFNELYDSVLSGKEAEIVITANSKSDYRKNLQRELQEIHDSECW
                     QAGKQVRKLRPKQV"
     gene            complement(4328..4849)
                     /gene="ilvN"
                     /locus_tag="PLG24_06135"
     CDS             complement(4328..4849)
                     /gene="ilvN"
                     /locus_tag="PLG24_06135"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HMZ24503.1"
                     /translation="MEKTFTVSIFTENSIGMLNRITIIFTRRHLNIDSITASESEVKD
                     VHRYTITLKTTREKIDKVLGQIEKLVDVIKAFVHEENEIVFQELALYKIKTASISNGE
                     VEQVIRDHHARILSVDPLFMVVEKTGHVADTQLLFEKLKPFGILEFARSGGVAVTKPM
                     KELSTYLKELKVK"
     gene            complement(4869..6557)
                     /gene="ilvB"
                     /locus_tag="PLG24_06140"
     CDS             complement(4869..6557)
                     /gene="ilvB"
                     /locus_tag="PLG24_06140"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HMZ24504.1"
                     /translation="MEKLTGAQAVIQVLLTEEITTVFGYPGGAIMPIYDALFDYSDEI
                     HHILVRHEQGAIHAAQGYARVTGRTGVVMATSGPGATNLITGLADAMIDSTPLVCITG
                     QVSSKLLGTDAFQETDVIGISMPVTKWNCQITRTEDIPSIMAKAFYIARSGRPGPVLI
                     DITKDAQFGVSDFSFEKCTHIRSYNPTPEIDYSSLEAAAELINQAKKPMMVIGQGIML
                     SEAEEALRTFADKSGIPVASTLLGLGAFPCNHPLFVGFVGMHGDYAPNIKTNECDLLI
                     GIGMRFDDRVTGDTSRYATQATIIHIDIDASELNKIIPTDVPVHADAREALLFLTHLV
                     KERSHESWVNEFKEAKQTEVETLSKGYANCDTQDVRMDEVIKLLSNLTHGESIIVTDV
                     GQHQMMAARYYQYNKTKSNVTSGGLGTMGFALPAAIGAKLGMPDKDVIAIIGDGGFQM
                     TLQELGTILQSKIPVKIVILNNNFLGMVRQWQEMFFERRYSFTEMISPDYVKLAESYG
                     IQAKSIASKTDLEDGLREMLDAKEAYLLEVKVVKEENVFPMVPTGASVSEIRLA"
     gene            complement(6594..>7511)
                     /locus_tag="PLG24_06145"
     CDS             complement(6594..>7511)
                     /locus_tag="PLG24_06145"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HMZ24505.1"
                     /translation="MTRRAFDNAIRMVIVLGGSTNAVLHLIAMADSVGIQLTLDDFQR
                     MSDSTPVLADLKPSGKYLMEDLHQVGGTPAVMKYLLDHGLLYGDCMTVTGHTLAENLA
                     SLPELSSGQDVLWPICNPVKTSGHIHVLYGNLATEGSVAKISGKEGQYFEGPARVYDD
                     EYKMIAGLSNGEIKPGDVIVIRYCGPRGGPGMPEMLKPTSAIMGAGLGNQVALITDGR
                     FSGGTHGFVVGHISPEAYDGGTLALLRDGDIITIDALHNTIDVHLEEEVLQRRKAEWV
                     LPELKARNGILFKYAKCVSGASTGCVTDK"
BASE COUNT         2094 a         1646 c         1436 g         2335 t
ORIGIN      
        1 tgcaattaat catatctccg gatatagtac cagtcatcat tatattggaa ttgttcccat
       61 caaataaaag cttattccct tctaacctac ctgataaatt atctaatacg ttatcgtaga
      121 tatcagttaa ttgaccagtg aatgttccat tatacatgat tattatttct cctttagtat
      181 caacattatt gctgaataaa tatcttccgc gatactcatt gacagaattt tgaattgctt
      241 ttccttcaac agtcttgtta gatttcccag aaaaagatga gttattatta cggcaatctg
      301 aaaataaagc caatacccca gaaaaaataa aaatgagaaa aattgcttta tgaatattcc
      361 ggtaatattg accccccatt ccggtgatat tgacccccct gggattttgg ttatttaaaa
      421 gaacgtttta cctgacaata ttacagtttt tttccgccaa catttgtaaa atctgcaagt
      481 aaaacaacat ctcctgatgt tactccatcc ttgctatcgc gacgttcaaa actaagagca
      541 ccactaattt ctgagtcccg aatggtgtaa agccccgaaa aattatcaga aacttccaca
      601 ttcttttgtt cattagtact attttctttt tgcgagactt tactgtcagc attttcacag
      661 gaaaataaca aaaacaatgt tatcagaatg aaaaaaggtc ttgaccaccg attgtaaaat
      721 ttaaataatt tattttccat agtataaatt tttgttatcg tcatttagtt tcttataaat
      781 tatgtaaaaa gttttcataa tacatattac actacaagtg atttcaattt tatcatccag
      841 accaacattc cttatttgat tttaatccgg atttatttaa atttttattg actggaaatt
      901 aaatcgaaat atataatcac agaataagat agatccttaa agtcagtttt aaactggttt
      961 taaactggtt gtaatctgat tatgacaatt atttcgaccc aaattttatt catacttgcc
     1021 atcagacaat gcggaaatgt attggtaaca agcattttaa caatactaat ttcattattt
     1081 tgaaaaagta gtgttaacct aaattaagcg atgggaattt aatactagaa aaaaattgta
     1141 atattctacc gcgtagcaat agacatttta tatgattgtt ttttccgtaa gctttaccaa
     1201 taaagagatt acgccatggg gcggaatggt gtttttgaag caaatgttgg acaaaatgca
     1261 gttttcgcaa attatttcca gttgtaagca cttaccagtt ccaggattta ataggggcta
     1321 cttacctgaa gtgaccgtgg agagctttat tgcctgttgc aaaataacca actccttggc
     1381 cagggtatac ctgatgcagc cctgttaaac caaaactgat tatccaaatt tagtcagtca
     1441 gatttctgga taggtaaaaa aatcaaattc aaaaagacga ctctctgatt ttacttagat
     1501 ttacaaaacc taattggaca gaaaaacgaa attaatccta attttacttc aaaagaaatg
     1561 gagataatac cggattcaaa acaactgatg accaggcgat acgggcggaa gataactatg
     1621 ggatttccgt aataattaat tataaatcag gacatgaact cccttctatt gacacatcat
     1681 ttcaatttta aaaataagtt taatacatcg aaagaaatat tatgaaaaag aactatttat
     1741 tcataggcat aagcgtgctg atcatcgctt tttcgataaa taatcttacc ctggacatag
     1801 ataaccgagg gcattatata ggtaatggaa ttctttgcgg tatcggcatc agcatattcg
     1861 ttacacagat ggtaagactt ataagaaata agtaagcaca gcaggaaaat ttttccgtac
     1921 ttgatttcag gagagattca tagtgtatca ttcagattac aaaacctgaa tgatacacta
     1981 taatccattt aaaaacgaat ttgtaaaaca gctattcgcc ctaaaaaatt aaatgtgtaa
     2041 agagatctgc attttcattg aggtattggt aattgatgtt gtgcacattt agattttcct
     2101 ttaacatgtt gaaatcgtct tgtcgtttta actcaatacc tactacggca gggccattct
     2161 cgcgattatt tttctgggaa aactgaaaat acgtgatatc gtcgttcggc cccagcacat
     2221 tattgacaaa ttctttgagt gcaccgggtc gttgtggaaa attgaccata aaataatgct
     2281 ttagcccttc gtacagcaaa gaacgttctt ttatttcttc tgtacgagtg atgtcattat
     2341 tgcccccact gatgatacac actacatttt taccttttat ttcatcttta taaagatcca
     2401 gtgcagcgat agtcaaggca ccggcaggtt ccacgatgat ggcttcttca ttatagagct
     2461 ttaaaatggc agaacatact ttgccttccg gaacagggat aatatcactt aatccctctt
     2521 ggcatatgcc aaaagtattg tccccaaccc tacttaccgc tgcaccatcc acaaacttat
     2581 ctatgtcggg aagtgcaatg acctgacccc gttcgacagc agatttcata gaggcagctc
     2641 cggcaggctc tacaccgata attttcgtct gtggactcag cttcttaaaa accgttatta
     2701 agcctgatgc cagtccaccg ccgcctattg ggacaaaaag atagtcaatc ggctgtgggg
     2761 catcatcgat aatctccatt ccgacagtac cttgtccggc tatgacatct tcatcatcaa
     2821 agggatgaat aaatatcgca ttcttacgat tgcaatactg tattgcttcc tgaaaagagt
     2881 catcaaaggt gtcacctttc aggattatat ctacatactc tttgccgaat aaccggactt
     2941 gccttagctt ctgctgcggt gtagtgacgg gcataaaaat acttgcgtag atacctaatt
     3001 tctgacaagc ataagcaaca ccctgtgcat ggtttcctgc actggcacat acgacaccac
     3061 gggcagattg gtcaggtgtg agtgtaataa ttttattaaa agctcctctt aacttataag
     3121 accttattgg ctgaaggtct tcccttttta gccaaatctg agcagaaagg gagtcactaa
     3181 agttttcatt tttaatcaag ggcgtcttta cagcaacacc cttgattttt tcctgcgcct
     3241 ggactacttt ctcgaataag ttcatacttg tttgggtctt aattttctaa cttgttttcc
     3301 ggcttgccaa cactcactgt cgtgtatttc ctgtaattct cgctgtaagt tttttcggta
     3361 atcggatttg ctattggcgg tgattactat ttctgcttct ttacccgata gtacgctgtc
     3421 atagagctca ttaaaaaccg gttctgtaac atctctgaac ctccccttcc aatcaagtgc
     3481 acctcgctga gccgtagtag aacaattggc aaacatccag tccataccat tctctgctac
     3541 gagtggcata aggctttggg tcagttcttc tactgtttca ttaaatgctt cactgggtga
     3601 atggcctcgc tgacgcaaaa cattgtactg tgcctcgaag ataccggcta tagctcccat
     3661 gagtacacct cgctccccgg taaggtcgct atatacctct ttttcaaaag tcgtttcgaa
     3721 caaatatccc gatccgatac ctatacccac tgccaaagct ctttccactg ctttaccggt
     3781 tgcatcctga tagacggcat aactggagtt taggccttgc ccacgaagga acaacgacct
     3841 cagtgaagtt ccggaaccct tcggtgccac taagattaca tctacatcct ttggcggttt
     3901 aatgcctgtc ttatcgtgaa aggtaacgcc aaaaccatgt gaaaagtaca atgcctttcc
     3961 ggcgtgtaaa tgtggtacca tcgtatccca agcctgaatc tgtccagcgt cagagagtag
     4021 attcataagg atggaacccc gggcgcatgc ttcttctata tcgaataagg tgctgccttc
     4081 cttccatcca tcggcaagtg ccttatccca gctcgcacta tgacgccgct gaccgactat
     4141 gacccgaaaa ccattgtctc tcaggttaag tgcctgaccg gggccttgca ccccataacc
     4201 gatcaccgcc attgtttcat cgtgtaaagt tgctttcgct ttctctaagg ggaagtcttc
     4261 acgtgtaacg acttcttcct gcacgccgcc aaaatttaat gttgccataa gtattatttt
     4321 gttgatttta ttttactttt aattctttca gataggtgct gagttctttc ataggcttgg
     4381 taactgctac accgcccgag cgcgcaaact ccagaatgcc gaatggtttt agtttttcga
     4441 agagcaattg ggtatctgcc acatgacctg ttttttctac taccataaat aatggatcta
     4501 ctgataatat gcgtgcgtga tgatcacgga ttacttgttc aacttcgcca ttagagatgg
     4561 atgcagtttt gatcttgtac agggccagtt cctgaaaaac aatttcattt tcttcatgga
     4621 caaaagcctt gatcacatct accagttttt ctatttgtcc caatacttta tctattttct
     4681 cacgtgttgt ttttagcgta atggtatagc gatgcacatc tttaacctca gattctgaag
     4741 cggtaatact atcgatattg aggtgtcttc ttgtaaatat gatggtaata cgattcagca
     4801 tgcctatgct gttctcggta aaaatggata ctgtaaatgt tttttccatt ggataaaata
     4861 ttatattgtt atgcaagtct gatttctgaa acggaggcac cggtaggcac catggggaat
     4921 acattttctt cttttactac tttcacttcc agtaaatatg cttctttcgc atccagcatc
     4981 tcacgcaagc cgtcttccag atctgttttg gaagcaatac ttttcgcttg tataccatag
     5041 gattcagcta gttttacata atccgggctg atcatttcgg taaacgagta gcgtctttcg
     5101 aagaacattt cctgccactg tcgcaccata ccgagaaaat tattgtttag tatgacaatc
     5161 ttcacgggta ttttactctg gagtatggtg cccagctctt gtaaagtcat ctgaaatcct
     5221 ccatcaccga tgatggctat tacgtcttta tcgggcatgc ccagtttagc acctatcgct
     5281 gccggaagtg caaatcccat ggtgccgagt ccacctgatg taacattgct tttggtttta
     5341 ttgtattgat agtatcgtgc tgccatcatc tgatgctgac ctacatcggt aacaatgata
     5401 gattctccat gagttagatt ggataaaagt tttatcacct catccattcg cacatcctgt
     5461 gtatcacaat ttgcatatcc ctttgatagc gtttctacct ccgtttgttt tgcctcttta
     5521 aattcattaa cccatgattc atgactcctt tcctttacga gatgtgtaag aaaaagtagt
     5581 gcttcccttg catccgcatg gacgggcaca tcggtgggta tgattttatt gagttctgag
     5641 gcatcgatgt ctatgtgaat gattgttgcc tgtgtggcgt agcgagacgt gtctccggtt
     5701 acacggtcat caaatcgcat tcctatacca atgagcaggt cgcattcatt ggtcttgata
     5761 ttgggcgcat aatcgccatg catacctaca aagcctacaa acaatgggtg attgcatggg
     5821 aaggcgccta aaccaagcaa ggtagaagcc accggaatac ctgatttgtc tgcaaaggtg
     5881 cgcaatgctt cttcggcttc agaaagcatg attccttgtc cgataaccat cattggtttt
     5941 ttagcctgat tgattagttc agctgctgcc tcgaggcttg aataatctat ttcaggtgta
     6001 ggattataac tcctgatgtg ggtgcatttt tcaaatgaaa aatcggatac accaaattgt
     6061 gcatctttgg taatatcaat caatacgggg ccgggacgac ctgaccgtgc gatgtagaaa
     6121 gcttttgcca ttatggatgg aatatcctcc gttctggtga tctggcagtt ccacttggtg
     6181 accggcatag aaattcctat gacatccgtt tcctgaaagg catctgttcc gagaagctta
     6241 gacgatacct gccctgtgat acagaccagt ggtgtagaat cgatcatcgc atcggctaat
     6301 ccggtaatga ggtttgtggc acccgggcct gatgtggcca tcactacacc tgtccttcct
     6361 gttaccctgg catagccctg ggcagcatga atagccccct gttcgtgtct taccaggatg
     6421 tgatggatct catctgagta gtcaaagaga gcatcataga tgggcatgat ggcaccgccg
     6481 ggataaccga aaacagtagt aatttcttcg gttaataaga cttgtattac tgcctgagca
     6541 cctgttaatt tttccattgt tctgtatatt tatctggatt tatataattg attttattta
     6601 tctgtgacac atccggtaga agctccggaa acacatttgg cgtatttgaa taaaattcca
     6661 ttccttgctt taagctccgg taggacccat tccgccttcc ttcgctgaag tacttcctcc
     6721 tcaaggtgaa catcgatggt attgtgcagt gcatcgatgg tgataatgtc accatcccgg
     6781 agtagagcta aagtgccacc atcataagct tccggagaga tatgacctac aacaaagccg
     6841 tgagtacccc ccgaaaagcg gccatctgta atcaatgcta cttgattgcc caggcctgct
     6901 cccatgatag ccgatgtcgg tttgagcatc tcgggcattc ccgggccgcc cctgggtccg
     6961 cagtagcgaa tgacgatgac atcgcccggt ttgatttcac catttgacaa tccggctatc
     7021 atcttgtatt catcatcata gactctggca gggccttcaa aatattgtcc ctccttgcca
     7081 ctgattttcg ctacacttcc ttcggtggca aggttgccat acagcacgtg tatatgtccg
     7141 ctggtcttaa ccggattgca aatcggccat aacacatctt gtccggagga taactcagga
     7201 agagaagcaa gattttccgc cagggtatga ccggttaccg tcatgcagtc accgtagagg
     7261 aggccgtgat ccaacaaata tttcataaca gcgggagtgc cacctacctg atgaaggtct
     7321 tccatcaggt atttaccact tggcttaaga tctgccaaaa cgggcgtgct atcactcatg
     7381 cgctgaaaat catctaaggt aagttggatg cctactgaat cggccatagc aattaaatgc
     7441 aataccgcat ttgtagagcc acccagcacg atcaccattc ttatcgcatt atcgaaagcg
     7501 cggcgggtca t
//
LOCUS       DAOQQN010000126         7476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3968_length_7476_cov_5.088937, whole genome shotgun sequence.
ACCESSION   DAOQQN010000126 DAOQQN010000000
VERSION     DAOQQN010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7476
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3968_length_7476_cov_5.088937"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PLG24_06150"
     CDS             complement(<1..1064)
                     /locus_tag="PLG24_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24506.1"
                     /translation="MLLIFALMVGVISINGQDIQKDSTGLPGDNFNLQGTLDLFSKAN
                     DLESFEKSINDEKNNVNNLDLNGDGQIDYIKVIDRTDGNAHAIVLQAVLGNNDNQDIA
                     VIEIEKKGNEDAVAQIVGDEDIYGKEVYVEANSDNSSIDDDGNQIRKGPAAYSPNTFR
                     HIYVNVWFWPCVQYIYSPRYIVYVSPWHWHSYPMWWRPWRPWHWHTFYHGGWHYHHHY
                     VVVSHPRVYGARKVYAPHRVVSNNVEVRNKTVINNYRIKNNIVIDKNDSRHRGSNNND
                     NPAIRENMNSRTRQSDPGNMQNGSGATKSDRNTTDQPRRDNINRNNSSDRPTRVDRPT
                     ENGTLSPSRDQQPAKREERYT"
     gene            1310..2911
                     /locus_tag="PLG24_06155"
     CDS             1310..2911
                     /locus_tag="PLG24_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24507.1"
                     /translation="MNWKFNYRLLSRITAAILLGIVLIRFFLINIVDTALRNQLEKIR
                     QPDLSIDYNGLSIGWYSPSVYIDHMEVVGMGQSDTAMKISVERIHFQDIRLRKLFFHK
                     EIIVGRISLDQTVLSTKTGQRMEWKNFPKQGNLNSGLKLFSKEFRFKNFQWNHFDERD
                     ELAMIAGTQRFSIFGIQYRSKALPGDGVLFDSIGCRDFSFVSPAIQHKITIGSVDYSR
                     NLKELNIGEVRVVPLLDKFEFAQYFKRQVDRIECHSREIKLTDVTYKDKGSMYLKAGK
                     MAFDFHMETYRNKTYPFNRKKASPMPRELLKKTGFIFDIDTFELHDGFIAYEELDSLY
                     NNPGRIYFKGLNIRANHFSNDSKYNGNTLRATSQFMESGVIEAEFFFPEDSAKKYRVQ
                     GSLSPMDLSEINGILKAASLFEVKTGTLDTMRFLFDYDRVEASGKVEFAFNDLKLVAY
                     KKKSPEKISIMKTLVINQVIRMNKVKNANLIGHIGFKRDPVRSVVSYWWRSLFSGIKS
                     AFVKDGSFNPTRNESGTLGPKFKDE"
     gene            complement(2924..5362)
                     /locus_tag="PLG24_06160"
     CDS             complement(2924..5362)
                     /locus_tag="PLG24_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HMZ24508.1"
                     /translation="MKKHSLIVTALFALLFFQMNAQVITPESFLGYKPGNRYTPHHTL
                     IAYFKSVAEKNNERVLLTQYGTSYEGRPLITVFISTAENIANLENIRKNHLALIEPDK
                     SEPDKNDKCIVWLSYNVHGNEASGSEAAMTTLHEILSDADFNNHYKDMIVVIDPCLNP
                     DGRERYTNWYTSMAGSRPDSKVWSREHQEPWPGGRTNHYYFDLNRDWAWQTQLESQSR
                     LKIYNDWMPQIHVDFHEQGYNIPYYFAPAAEPYHEAITPFQRSFQKTIGQEIASEFDK
                     NGWLYFTRERFDLFYPSYGDTYPIYNGAIGMTFEQAGGKNAGISVMTKDGELLSLYDR
                     IAHHTTSGMSTIKVVRDNIAKINSEFFKFFHTGINTQHYHFQIKETDYERARSFKSLL
                     DKNKIRYRFAQHSDRSATLTIPLKQPKSTLIKILMEENSRLTDSLTYDITAWNLPKAF
                     GLNYSITANPMDIKNPPKTIKPTATAYGYAIAWQGGNVSKALSWMLQQGIRLRFSENE
                     FSLEGTKFDRGTLLILDSGSNRDKNIREVMDSVVNKFDIAIKPISRGMVDTGNDMGSE
                     SIRVINAPKVILLSGEGVFSLSLGEIWFHMDMELDFPVSLVNTSQLRNFNWSDADIII
                     LPDGNYSFLRDKELTDQLENWIRRGGKLIAFENAVNQLTGLNWVHLKKKDSPADSTSA
                     NKYSSLKKFGDRERAELKNYIPGSIYRIDLDNTHPLAFGYPSYYYSLKLNSTFYNYIK
                     DGGWNVGVIKGGTEKSGFIGSNVRSEFKDAFIFGVEQVGKGNIVYFTDNILFRNFWEN
                     GKLLFDNALFLVGQ"
     gene            complement(5719..5791)
                     /locus_tag="PLG24_06165"
     tRNA            complement(5719..5791)
                     /locus_tag="PLG24_06165"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5756..5758),aa:Lys,seq:ttt)
     gene            complement(6182..6337)
                     /locus_tag="PLG24_06170"
     CDS             complement(6182..6337)
                     /locus_tag="PLG24_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24509.1"
                     /translation="MPQYDYHASINYTTETRLLIEDYPLAYLRVGRPPDAQNRYSSKN
                     HLRTDGA"
     gene            complement(7162..>7476)
                     /locus_tag="PLG24_06175"
     CDS             complement(7162..>7476)
                     /locus_tag="PLG24_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ24510.1"
                     /translation="VKHTAKSYAYCSDTVYLPSIIPIIKDVDLLYHEATYLDDFEEKA
                     KERGHSTARQAGMLARQAGVKKLLLGHPSSKYANIEALVKEAQNEFQNTKYATEGLTF
                     EV"
BASE COUNT         2172 a         1344 c         1550 g         2410 t
ORIGIN      
        1 gtataccttt cttccctttt tgcaggttgc tgatcccttg aaggtgacaa ggtgccgttt
       61 tcagtcggac gatccactct ggttggtcga tcggagctgt tgttccgatt gatattgtcc
      121 cttcgtggtt gatccgttgt atttctatca gattttgttg caccagaccc gttttgcata
      181 ttgccggggt cggattgcct ggttcgacta ttcatattct cccggattgc cggattatca
      241 ttgttgttgc ttccgcgatg gcggctatcg tttttatcaa taactatatt atttttgatt
      301 ctgtagttat tgattaccgt tttatttcta acctctacat tatttgatac aaccctatgg
      361 ggggcataga ccttacgtgc accataaact ctggggtgac taacgacaac gtaatgatgg
      421 tgataatgcc accctccatg gtagaatgta tgccaatgcc atggacgcca tggacgccac
      481 cacattgggt aggagtgcca gtgccagggt gaaacataga caatatatct cggtgaatag
      541 atgtattgga cacaaggcca aaaccatacg ttgacatata tgtgccggaa tgtattcggg
      601 ctgtatgctg ctggaccctt cctgatttgg ttcccgtcat catcgatact tgaattgtct
      661 gaatttgcct caacataaac ttcctttccg taaatatctt catctcctac tatttgagcg
      721 actgcatctt catttccttt tttttcgatt tcaataacgg caatatcctg attgtcattg
      781 tttccaagca ctgcttgtaa tactatagca tgtgcattcc catcagttct atcgattact
      841 ttgatataat caatttgtcc atcaccgttc aggtcgaggt tatttacatt gtttttttca
      901 tcgttgatgg atttttcgaa tgattcaagg tcattggctt tactaaacaa atccagggtc
      961 ccctgaaggt tgaaattatc acccggaaga cctgttgaat ccttttggat atcctggcca
     1021 ttgattgaaa tgacacctac catcaaggca aaaatcaaaa gcattgatct atttattatt
     1081 ttcatggcat aaattggttt atgattgaat aattgacttt ttttattact ttgatactta
     1141 aaaaacgtat tggtttatcg gaaagtaacc aggttttcat taaagttgag ttaaagattt
     1201 ttcaataatg ttaaaaaaaa tcaaagaata aatatacagg ctctttaaat gttaaacctt
     1261 tattttaagc cgatatgcac caagatgaaa agcttatctt tacctttaaa tgaattggaa
     1321 gttcaattat cgattactca gcaggatcac tgcagccatt ctcttaggaa ttgtcctcat
     1381 taggtttttt ctaatcaata tcgttgatac ggcactccga aatcaactgg aaaaaatcag
     1441 acaacctgat ttgtccattg attataatgg actgtctatc gggtggtaca gtccatcggt
     1501 atatattgat cacatggaag ttgtagggat ggggcagtcc gatactgcaa tgaaaatatc
     1561 tgttgaacgc atacacttcc aggatatcag actccgtaaa ttgttttttc acaaggaaat
     1621 tattgttggc cgtatttcac tggatcaaac agttctttct accaaaacgg gccaacgcat
     1681 ggaatggaag aatttcccga aacaaggaaa tttgaattcc gggttgaaat tgttcagtaa
     1741 ggaatttagg tttaaaaatt ttcaatggaa tcattttgac gagagggatg aattggccat
     1801 gattgcaggg actcaaaggt tttcaatatt tgggattcaa tacagatcaa aggcgcttcc
     1861 gggcgatgga gttctttttg atagtatcgg ttgtagggat ttttcttttg tatccccggc
     1921 aattcaacat aagattacga ttggcagtgt tgattattca agaaatttaa aagaactgaa
     1981 tattggggaa gtaagggtgg ttcctttatt ggataaattt gagtttgcac aatactttaa
     2041 aaggcaggtt gaccgaattg agtgtcattc cagggaaatt aaacttacgg acgtaaccta
     2101 taaagacaag ggtagcatgt atcttaaagc gggaaagatg gcttttgatt ttcatatgga
     2161 aacctacaga aataagacct acccctttaa cagaaaaaag gcaagcccaa tgccaaggga
     2221 attgctgaag aagacgggtt ttatattcga tattgacacc tttgagctac atgacggttt
     2281 tattgcctat gaagaattgg attccttgta taataatccg ggaagaatat actttaaggg
     2341 attgaatatt agggcaaatc acttttccaa tgattccaaa tataatggaa atacactccg
     2401 ggcaacaagc caatttatgg aatcaggtgt cattgaagct gagtttttct ttccggaaga
     2461 ttccgcgaag aagtacaggg tgcaagggtc attgtcaccc atggatttga gtgaaatcaa
     2521 cggaatcttg aaggctgcat ctctttttga agtaaaaaca ggtacactgg atacgatgag
     2581 atttcttttt gattacgata gggtggaagc atcaggaaag gtggaatttg ccttcaatga
     2641 cttgaagctt gtggcataca aaaagaagtc tccggaaaag atttccatca tgaagacttt
     2701 ggtaatcaat caggtcatca ggatgaataa ggttaaaaat gctaatctga ttgggcatat
     2761 tggatttaag agggatccgg taagatccgt agtgagctac tggtggcgct cattattttc
     2821 tggaattaag tctgcctttg tcaaagatgg gtcatttaat ccaaccagga atgagtcggg
     2881 gaccttaggg ccaaaattta aagacgaatg agctgtctct tgcctactga cctaccagaa
     2941 acagcgcatt gtcaaacaac agtttcccgt tctcccaaaa attcctgaat agaatattgt
     3001 ccgtaaaata aacgatattg cctttcccaa cctgctcaac gccaaatata aaagcgtcct
     3061 taaactctga ccggacattg gatcctataa atccagactt ttctgttcct ccttttatga
     3121 cgccgacatt ccatccgcca tccttgatat aattatagaa tgtactgttg agtttgagtg
     3181 agtagtaata ggatggatac ccgaaggcta gtggatgggt gttgtccaga tctatcctgt
     3241 atatcgatcc gggaatataa ttcttcaatt cagcccgctc tctgtctcca aattttttca
     3301 aagaagaata cttgttggca cttgtggaat cagccgggct atccttcttt ttaaggtgta
     3361 cccaatttaa tccggtgagt tggttgactg cattttcaaa tgcaattaat tttcctcctc
     3421 gccggatcca attttccaac tgatccgtta attccttgtc tctcagaaaa gaataattcc
     3481 catcaggcaa aatgataata tcggcatcgg accaattaaa atttcgcagt tgagatgtat
     3541 tgaccaggct tacaggaaag tctaactcca tatccatatg gaaccaaatt tcacccaacg
     3601 acaaggaaaa gacgccttcg cctgacagca ggatgacttt cggggcattg atgactctaa
     3661 tggattcgct acccatatca ttccctgtat ctaccattcc acgggatatt ggttttatag
     3721 caatatcaaa cttattcact accgaatcca tcacttcacg gatatttttg tcgcgattgg
     3781 atccgctatc taggatcaaa agagtgcctc tgtcaaattt tgttccctcc agactaaatt
     3841 cattttctga gaatcttaaa cggattccct gttgaagcat ccatgacaag gctttactta
     3901 cattgcctcc ctgccaggcg attgcatagc cataagcagt ggctgtgggc ttgattgttt
     3961 tgggtggatt tttaatgtcc atcggatttg ctgtgattga gtaattgagg ccaaatgcct
     4021 ttgggagatt ccaggctgta atgtcataag ttaaggaatc ggtcagcctg gagttttctt
     4081 ccataagaat cttgattaat gtcgatttgg gttgtttaag gggaatggtt aaagtagctg
     4141 atctgtctga atgttgagca aatcgatatc gaattttgtt tttgtccaac aaggatttga
     4201 agcttctggc tctttcgtaa tctgtctctt ttatctggaa gtggtaatgc tgagtattga
     4261 ttccggtatg aaaaaacttg aagaattcag aattgatttt ggcaatatta tcccgaacaa
     4321 ccttgatggt tgacattcct gaggtggtgt ggtgggcaat gcgatcgtaa agtgaaagga
     4381 gctcaccatc ctttgtcatg acagaaatgc ctgcattttt tccaccggct tgttcgaacg
     4441 tcattccgat ggctccatta tatatcggat aggtgtcccc ataggatggg tagaacaagt
     4501 cgaatctttc tctggtaaaa tacagccatc cgtttttgtc aaattcgctt gcaatttcct
     4561 gtccaatcgt tttttgaaaa ctacgttgaa atggtgttat cgcttcatgg taaggctctg
     4621 cggctggtgc aaagtaatag gggatattgt atccttgttc gtggaagtca acgtggattt
     4681 gaggcatcca gtcattgtat atttttagtc gactttgtga ttccaattgt gtctgccatg
     4741 cccaatctct gttcaagtca aaatagtaat gattggttct gccaccgggc catggttcct
     4801 gatgctctct ggaccatact ttgctatccg gcctggatcc ggccattgag gtataccagt
     4861 tggtgtaacg ctcccttcca tccgggttca ggcaaggatc gattactaca atcatgtctt
     4921 tataatgatt attgaaatct gcgtctgaaa ggatctcatg cagagttgtc atagcagcct
     4981 cacttccgga tgcctcgttg ccatgaacat tatagcttag ccacacgatg catttatcat
     5041 ttttatccgg ttcggattta tccggttcga tcaacgcaag atggtttttc ctgatgtttt
     5101 ctagatttgc aatattctca gctgttgaga taaaaacagt tatcagaggt ctaccttcat
     5161 aagaagtacc atattgggtt aagaggactc tttcattatt cttttcagca acggatttga
     5221 agtaggcaat cagggtgtgg tgaggagtat agcggttgcc tggtttatag cctaaaaaac
     5281 tttcaggggt aatgacctgt gcgttcattt gaaagaacag gagtgcaaat aatgcagtga
     5341 caataagaga atgttttttc attcagataa aatttatcaa ataaagatag tgaaaaaaag
     5401 taaaaaacaa aagttatagc aggaggagtg tactacaaat tgcacttttc tgttggatag
     5461 aagactggtt ttgtgtacag taaaaatata agttttggtg aaaaattata gggtaatgaa
     5521 aaaatgttat taaaataatt agatctcttt ttcttaattt aacagtatac aaatataatt
     5581 aaactgtcag attaagaatt aactattaca tgtaatgatc tgattgatac aggactatta
     5641 gaggatgtat gccggagatg taaggtgtat tttgataaaa tcaaaaaggt ttcaggaaaa
     5701 ctcctgaaac cttttttgtg atcccgctgg ggttcgaacc cagggcccca acattaaaag
     5761 tgttgtgctc taccggctga gctacaggat cgttctttta aattttcttt aaaagcggtg
     5821 caaagatata ttgaaataat taaatcccaa aacatatgtt gcctttattt taagaaaaat
     5881 ctcacatttt taaaatgagg cagcgacata tttgattatt agacgattat acttaggctt
     5941 cgcagcgatt tttcgtaaac ccatgatggc ttggtttatt agggttgcaa caatggaatc
     6001 ttgtaaaatt agtgaaagaa ccggaagaat ttttcgaagg aatctgtgag gcaagatata
     6061 tctaaagctg agcttgttat acatcgaact gcgaaaatca gggctatttg aagcgaaatc
     6121 aaaggtgcag gccctatgaa aatcccaaag ccgagtagaa gattattccc atatacaagc
     6181 cttatgcgcc atctgttctc aaatgattct tgctggaata tcgattctga gcgtctggcg
     6241 gtcttcctac tctcaaataa gccaagggat agtcctctat caatagcctt gtctctgtcg
     6301 tgtagttgat acttgcgtgg taatcatatt ggggcatttt aattcacctt gattaaaaag
     6361 ctgttatttg ggttataaat tcaaaatatc atattaaata taaaattgat atatatgtaa
     6421 atatttatta tgatgtgtat tggattgttt tcttgcagat ttggcatccc atgaattatt
     6481 atacattata agacctatat cagtagagaa gagtattggc acataattca tcggagattt
     6541 ggattcaatc aataaaaggg aatagtcctt cacgaggttg atctctacac aagatgctgt
     6601 tcagaaaatt aaaatgcatc gggtttggtg agccaggcgg gaaatagaga gaaatggagc
     6661 tttgtcttga gcgtaaagaa tgtttccaat gtaaattatg acagagatta taccggattg
     6721 tattgatcgc agatccggac cacctgacac aaattgcaat caaacaacga ggaatggatt
     6781 gggtgcttat ctattgattt ttcgacttat agtttggttc taaatgtttg aaaatgagat
     6841 gggtaaagcg gtgaatggct gggagggtga gctctttaaa taggtagatg aataatacga
     6901 caaatgtata ctatttgtga gattggtaaa aaagtgcagt aaaagtatgt ttaatgtatg
     6961 cttactttca ggtttgactc tcataaagta tgttgaatca aacgcttgtt gtattatttt
     7021 catacctact taaatttggc gccaactacc caatgctatt aagtccatat ttaaaaacca
     7081 cttaagaagt gaagatttat ttttgaaaga taattcagct caagtataag tattcataca
     7141 taggcaggta tctggaatct attaaacttc aaaagtcaga ccttcagttg cgtatttggt
     7201 attttgaaat tcattctgtg cttcttttac caaagcttct atgttggcgt atttgctgga
     7261 agggtgaccc agaagaagtt tttttacacc ggcttgtcgt gccaacattc cggcctgtct
     7321 tgcagtacta tgtccccttt cttttgcctt ctcttcaaag tcatccagat acgtcgcttc
     7381 atgatacaaa agatccacat cctttataat aggtatgatt gaaggaagat ataccgtgtc
     7441 gctacagtaa gcataggatt ttgctgtgtg cttgac
//
LOCUS       DAOQQN010000127         7449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_3992_length_7449_cov_4.921152, whole genome shotgun sequence.
ACCESSION   DAOQQN010000127 DAOQQN010000000
VERSION     DAOQQN010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3992_length_7449_cov_4.921152"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2862)
                     /locus_tag="PLG24_06180"
     CDS             complement(<1..2862)
                     /locus_tag="PLG24_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HMZ24511.1"
                     /translation="MTETLTHTSAENIIERLSTDENDPRQMAEICDSLFQHLLDHATE
                     AFRMPFNTVFSQLAFITSQFKVEHTRTRALHYYRLNHLHHPDKSDEENLKIRRGILSL
                     LNDMVFGTKTERFADLQTYALRYARSPKNYSGVVREMRFSITEAHLEEGFFLGWNEDD
                     PDAIRKVVIDEASREALLTELMEDLNYFSFPMTVTLINVNISGNEYHPECIILLPDFL
                     IDVTAIAECYSYDGVNPRLHFIKKLMPSHPSDSLFLGLCANSFLDELVFRPNLDFKEL
                     IPRLFKISPLYLASLSDDKVIELVNQAKTHYHNILDFVRNYREGSREELQRALIEPSY
                     CSPTYGLQGRLDLYIDSEDNRSIIELKSGKLFRENKYGINQSHYIQTLLYDLLVRKNE
                     KSDKDLRAFILYSRLEGDRLRFAPPVRSLQLDALMTRNRILTMEYRFALIGANGNDPT
                     DVFGLCLELMREGQQGYIAQNLKFLHDTLNALTSVELKYLKAFTGFVAREYFTSKSGL
                     MRDRSTTGQAALWLNTAEEKAKDHLIMAGLLPESVEITHRDTLITFRKSPMTDLLADF
                     RIGDIVVLYAPDKEGNLRWEGRQLFRGTLVENNSFDVTIRLRARQNRKDLIFADRHWN
                     MEKDVIDSGITGQFRSLISWAASDVYNRKLLLGLIPPAKPERRIEIADNDISPYHREL
                     LENALSAPDYFLLWGPPGTGKTRYMVKNLIQLVIANTEKPVLVLAYTNRAVDELCNAI
                     ESISPEYCEKYIRIGSRYGTDPSFVGNLLDEKLLEISNRKQLLELLHTRRIIIGTASA
                     LLGKTELFSLLSFEWALIDEASQILDPQIIELLSYVPKFIMIGDHKQLPAVVTQHRSR
                     CLVTDREMNDCGIFDLSQSLFERLYLRAIQQGWDWAYGVLHRQGRMHSDIMTFPARHF
                     YDNRLETLFEHQIAQDFLRPATEAGHSGRKAELSRRRMLF"
     gene            3496..3569
                     /locus_tag="PLG24_06185"
     tRNA            3496..3569
                     /locus_tag="PLG24_06185"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3530..3532,aa:Pro,seq:ggg)
     gene            3930..5876
                     /locus_tag="PLG24_06190"
     CDS             3930..5876
                     /locus_tag="PLG24_06190"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMZ24512.1"
                     /translation="MSNKFLLVLFITFVSNYVFGQKDEVLFTVNKTPVTVSEFKYIYE
                     KSNNKNADYSEKSLQESLDLYTRFKLKVAKARDLKMDTLPSLRSELDSYKRQLADSYL
                     MDKEINEKLAKELYDRMQTDARVAHLFIAAGTDDTIKPLLKINEIKVRLNKGDKFEDL
                     VKLYSEDESSKKNGGDLGFLAPMYPGGFYSLENLVYSLKKGETGGPVKTSAGWHFVKI
                     LDTRTARGEMEIAQILVRITKDFDEGFAKDRIDSIYAMLQKGGDFAEIARKYSGDLAS
                     APKGGFLGFITIGQYDNAFEDPVFALTKDGEYTKPFKTALGYHIVKRISKKDMSDFNS
                     VKKSLMTKVSQNERFGIAKHKLVEDIKKEYHFTQDNKVLGQYIDSVNTDRFYSNIWTA
                     PGIPKATIFSIGENKYTTDDLSKYLVDNTRRRVRMNKSVLPSIAFNELFDEFVSNKAI
                     EYEQSRLEYKYPEYKALTREYEEGFLFFEVTNNEIWNKASQDSVGIEKYFNEHRDKYM
                     WEERARVLHYSIKASGPEELNRIFELARKYTPEELQKKYSLDKVSYTSETLEKSSASD
                     MKGLKWKQGEMSELHRNPDGRTGTFDKVESIMQPVRKELKDARGFVIADYQDFLDKQW
                     VEKLKQEYSVTVNKNVFNSLIKHK"
     gene            5886..7232
                     /locus_tag="PLG24_06195"
     CDS             5886..7232
                     /locus_tag="PLG24_06195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMZ24513.1"
                     /translation="MTRIFSVLALFMPIMCLAQPKVLNKVIAQVGGETVLLNEVEDNY
                     LGQTRGMTNVPPDLKCQIFEGLLLQKLLTNQAKIDSVKVSDEQVENQLNARFDRVLAY
                     MNNDMAQFEEYYGKSVNEMKDELREDLKDQMMAEEMKNKVVDKITITPSEVKAYFKSI
                     PHDSLPYYNSEVEYSELVYYPKPNKLERQRVIDKLNSIRTRIVENGEKFEDLAKKFSM
                     DGSAAAGGDLGWAKRGAFVPEFEAVVFNLEEGQVSTIVETEFGFHLIQLLERRGNSFH
                     ARHILIKPELSSSDKEAALKMLDSIVLQIRTGKLSFPDAVKKFSNKNYPSYSNGGRAI
                     NPHTNTNFFEISDLEPDVYFALDTMKIGGMSAPIELKDPYGETAYQVLLLQARTDPHR
                     ASLEKDFSRIKESAMMYKKEAELDNWVTTKIRDTFITIDPEWLGKCPGVQKWVKAN"
BASE COUNT         2100 a         1543 c         1747 g         2059 t
ORIGIN      
        1 aaacaacatc cttctccggg atagctctgc cttccggccc gaatgcccgg cctctgttgc
       61 aggcctcagg aagtcctgtg ctatctggtg ctcaaatagg gtttccagtc tgttgtcgta
      121 gaaatgtctt gccggaaaag tcatgatgtc actgtgcatc cgcccttgcc ggtgtagaac
      181 accatatgcc caatcccaac cctgttgaat ggcgcgcaga taaagccgtt cgaagagaga
      241 ttgtgacagg tcgaatatgc cgcaatcatt catttcccgg tctgtaacca gacacctgct
      301 cctgtgctgt gtcactactg caggcaattg cttatggtcg ccaatcatga tgaatttcgg
      361 cacataactc aacaattcaa tgatttgtgg gtcgagaatc tgactcgcct catcgatcag
      421 tgcccactcg aaggatagta ggctgaacag ctctgtcttg cccagcagtg cagaagcggt
      481 gccgataatg attctcctgg tatgcagtaa ttctagaagt tgctttcggt tactgatttc
      541 cagtaatttt tcatcgagca ggttgcccac gaaggatggg tcggttccat accgcgaacc
      601 aatacggatg tatttctcac agtattccgg gctgatggat tctattgcgt tgcacaattc
      661 gtctacggcc cgattggtat aggcaaggac caacacgggt ttttcggtat tggcgatgac
      721 caattggatg aggttcttga ccatatatct cgtcttacct gttcccggtg ggccccacag
      781 caggaagtaa tccggtgcgg acagggcatt ttccaacaat tcgcgatgat agggagaaat
      841 atcattatca gcaatctcaa tccgtcgttc aggcttagca ggaggaatca atcccagcag
      901 cagtttacga ttgtagacat ccgaagctgc ccacgaaatc aggcttctga actgaccggt
      961 aatgcccgaa tctatgacat ctttttccat gttccagtgc cggtcagcga atatgagatc
     1021 cttcctgttc tgcctggctc tgagccggat agtcacatcg aaggaattgt tttcgaccag
     1081 ggttcctcta aacaattgtc tgccttccca cctcaaatta ccctccttgt caggagcata
     1141 aaggacgacg atgtctccta tcctgaaatc agccagtaag tccgtcatag gcgactttct
     1201 aaaggtgatc agggtatccc ggtgggtaat ttctacactt tccgggagca gtcctgccat
     1261 aatcagatga tctttggcct tttcctcagc cgtattcaac caaagtgctg cctgcccggt
     1321 cgtacttcga tcgcgcataa ggcctgactt ggaagtgaaa tattcgcggg cgacgaaacc
     1381 tgtgaatgct ttgaggtatt taagttccac tgaggtcagt gcattgaggg tgtcatgcag
     1441 gaacttcagg ttttgtgcta tgtacccctg ctggccttca cgcatcaatt ccaaacacaa
     1501 gccgaagaca tccgttgggt cgttgccatt ggccccgatg agggcaaatc ggtattccat
     1561 tgtgaggatt ctgttgcggg tcattagtgc atccagctgg agtgagcgta cagggggagc
     1621 aaaacgcagc ctgtccccct caagcctgct gtacaggatg aaggcccgta agtctttgtc
     1681 cgatttctca tttttccgga ccagcaggtc gtaaagaagg gtctgaatgt aatggctctg
     1741 gttgattccg tatttgtttt cgcggaaaag cttcccggat ttcaattcga tgatggaacg
     1801 gttgtcttcg ctgtcaatgt ataggtccag acggccttgt aatccatatg tcggactaca
     1861 ataactcggt tctatcagtg cgcgctgcaa ctcctcccgg ctgccttccc tgtaatttcg
     1921 tacaaaatcg aggatgttgt gatagtgtgt cttagcctgg ttgactaatt ctattacttt
     1981 atcgtcactg agggaagcca ggtaaagcgg acttatcttg aataatcggg ggattaactc
     2041 tttaaagtcc agattaggcc ggaataccaa ttcgtctaag aatgagttgg cgcaaaggcc
     2101 taaaaacaaa gaatcggaag gatgggaagg catcagcttc ttgatgaaat gcaacctggg
     2161 attgacgccg tcataactgt agcattcggc gattgcggta acatcgatta gaaagtcagg
     2221 aaggaggatg atgcattcag gatgatattc attcccactt atattgacat taatcagggt
     2281 gacggtcatc gggaagctga aatagttcag gtcttccatc agttcggtaa gcaaagcttc
     2341 gcggcttgcc tcgtcgataa ccaccttgcg tatagcgtcc gggtcatctt cattccagcc
     2401 cagaaaaaag ccttcctcca gatgagcctc ggtaatggaa aagcgcattt cccgaactac
     2461 tccggaatag tttttgggag aacgggcata gcggagtgca tatgtctgca ggtcggcgaa
     2521 tctctcggtt tttgtaccaa aaaccatatc attgagcagg ctcagtatac cacgtcggat
     2581 cttcaggttt tcttcatcag acttatccgg atggtggaga tggttcagcc ggtagtaatg
     2641 aagtgcccgg gttcgagtat gctcgacttt aaattggctg gttatgaaag caagctgtga
     2701 aaatactgta ttgaaaggca ttctgaaggc ctctgtcgcg tggtccagca agtgttggaa
     2761 cagactatcg cagatttcgg ccatctgacg cggatcattc tcatccgtcg aaagcctttc
     2821 gatgatattt tccgcgctgg tgtgggttag tgtctcggtc ataattgtga aaataatata
     2881 ttaactggaa attaaattaa attgaataaa ttacccggat attgactcca ccatctctaa
     2941 tcattgaaat tttattaagg taaaatcctg gcttgaaata aaaacgagaa aatatccgga
     3001 taagcataac gggtaaattt gaaattgtaa aatgcgaagc tacggaaatt ttattgaaat
     3061 gtatcttcga tttgaatcaa tttgttattc ataccaaagg ttataactat atctgaaatc
     3121 attgatcccc gctaattatg ctgagaaaca aatgatgttt tccttgtgtt tattatattt
     3181 tatcatgtac acagctttac gggatacttt tgaactgtta taaaacaaat tgcaatttac
     3241 agagtgttta tgtataagtt ataaagccgc atttgggggc tgaatactgc ataatattaa
     3301 aatgatacta ttgagtcgtg ccaccggtac tttgggttgg ctatctgtaa atgataatca
     3361 gctggcagag ggtaaggaat tgaactatac ttcaagggtg tgaatgattg cccgggtgca
     3421 ttatcattca ctctattgta ctgattggtt agaattttct tgcttatagt ggaagattta
     3481 ttattttcgc ctcatcggga tgtagcgcag tctggtagcg tacacgtctg gggggcgtgt
     3541 ggtcgcaggt tcaaatcctg tcatcccgac ctgttagaag gcttcggttc tgatcgaagc
     3601 ctttttcttt tgctgttgcc atgactttgt atatcagtct ccgaatattt tctgatgcct
     3661 gccatgtgcc aatgaggtcg agtatgcagc agtgtcctat taacaaatct ttattatttg
     3721 aatttggcaa aaaaagtatc tttgcactcg ccaagtaagg gccaaaatgc aagtgtagca
     3781 aggattttaa taaaacgatc ttccttttgc tgtctacaat ggcctgattg gtccggtaat
     3841 tttatgaata ttcaggcgtt tttttcgttt attgaaatag ctttgggaat ccatgatatt
     3901 atttttttcg attaaccatt acaattcgta tgtctaataa atttctttta gtgcttttta
     3961 tcacctttgt ctccaactat gtttttggtc aaaaggatga agtacttttc acagtaaaca
     4021 aaactccggt aacggtcagt gaatttaaat atatctacga gaaatctaat aataaaaacg
     4081 ctgactattc tgaaaagtca ctacaggaga gccttgatct gtatacacgt ttcaagctca
     4141 aggtagccaa ggcacgcgac ctcaagatgg acacccttcc gtcccttcgt tcggaactgg
     4201 attcttacaa gcgccaattg gctgactcct atctgatgga caaggaaatc aatgaaaaac
     4261 tggctaagga gttgtacgac aggatgcaga ccgatgcccg ggttgcgcat ctctttatag
     4321 cagcaggaac agatgatacc atcaaacctt tactaaaaat caatgaaatc aaggtcaggc
     4381 tcaataaggg cgacaaattt gaagatctgg ttaaattata ttctgaagat gaatcatcaa
     4441 agaagaatgg tggagacctc ggttttcttg ctccgatgta tcccggcggg ttctactcat
     4501 tggagaatct ggtgtacagt ctgaaaaagg gggagacagg aggcccggta aaaacttctg
     4561 caggttggca ctttgtcaag atattggata cacgaactgc acgcggtgag atggagattg
     4621 cacagattct ggtgcggatt accaaggatt ttgacgaagg ttttgccaaa gacaggatag
     4681 actccatcta tgctatgttg cagaaaggcg gtgattttgc tgagattgcc agaaaatatt
     4741 ccggcgatct ggcatctgct cccaagggcg gctttctggg cttcatcact atcggtcagt
     4801 atgacaatgc ttttgaggat ccggtatttg ctttgacaaa agacggagaa tacaccaagc
     4861 cattcaagac agcactgggt tatcatatcg ttaagcgcat ctccaaaaaa gatatgtccg
     4921 acttcaattc tgtgaagaaa tcattgatga ccaaggtcag tcagaatgaa cgattcggta
     4981 ttgcaaagca caagctggtg gaggatatca agaaggagta tcatttcact caagacaata
     5041 aggttttggg gcaatacatc gattcggtca atacggatcg cttctattcc aatatttgga
     5101 ctgctcccgg catacctaag gctaccatct tcagtatagg agaaaataaa tataccacag
     5161 atgatcttag taagtatctt gtcgataaca ctcggcgaag ggtcaggatg aataaaagcg
     5221 tgttgccatc tatagctttc aacgaattgt ttgatgaatt tgtatccaat aaggcaattg
     5281 aatacgaaca gagccgactc gaatacaaat atcctgaata taaagctctt accagagagt
     5341 atgaagaagg attcctcttt tttgaagtta ccaataacga gatctggaac aaggcgtcac
     5401 aagacagtgt aggtattgaa aagtacttca atgagcatcg ggacaaatac atgtgggaag
     5461 aacgagccag agtcctgcat tatagtatta aggcttccgg accggaagag ctgaaccgca
     5521 tctttgaatt ggccaggaaa tatacacctg aggaattgca gaaaaaatat agcctggata
     5581 aggtttccta tacatctgag actctggaga agtcaagtgc cagcgacatg aaaggcctga
     5641 agtggaaaca gggtgaaatg tcagagttgc atcgaaatcc tgatggcagg actggtacct
     5701 ttgacaaagt ggagagcata atgcaaccgg tcagaaagga actgaaggat gcgcgaggtt
     5761 ttgttatcgc tgactatcag gattttctgg acaagcagtg ggttgaaaaa cttaagcagg
     5821 aatacagcgt taccgtcaat aaaaacgttt tcaactcact tatcaaacat aaataactga
     5881 tcggaatgac gcgtattttt agtgttttag ccctatttat gcccataatg tgccttgctc
     5941 aacctaaggt acttaacaaa gtcatagccc aggtgggtgg tgagactgtg ctgctcaatg
     6001 aagtagagga caattacctt ggccagacca ggggcatgac taatgttccc cctgatctga
     6061 aatgtcagat ttttgaaggt cttttgttgc aaaaactcct caccaatcaa gcaaagattg
     6121 actcagtaaa ggtcagtgac gagcaagttg aaaatcagct aaatgcccgt tttgacagag
     6181 tacttgccta tatgaacaac gatatggctc aatttgagga atattatggc aagtcggtca
     6241 atgagatgaa ggacgaactg cgtgaagatc tgaaagatca aatgatggca gaagagatga
     6301 aaaacaaggt ggtggacaag ataaccatta ctccatccga agtcaaggca tatttcaaat
     6361 ccattccgca tgactctctt ccctactaca actcggaggt cgaatacagc gaattggtct
     6421 attaccctaa gccaaataaa ctggaacgcc aacgtgttat cgataagctt aacagtatcc
     6481 gcacgcgaat cgtggagaat ggcgaaaagt ttgaagacct ggcaaagaaa ttttccatgg
     6541 acggttctgc tgctgcaggg ggagacttgg gctgggccaa gcgtggtgcc tttgtaccag
     6601 aatttgaagc tgttgtcttc aacctggaag aagggcaggt gagtacaatc gttgagacag
     6661 aattcgggtt tcacctcatc caactattgg aaagacgcgg gaattccttc catgctcgtc
     6721 atatacttat caagccggaa ttatcatctt ccgacaagga ggctgccctc aagatgctcg
     6781 atagtatcgt attacagatc aggaccggga agcttagttt tcctgatgct gtaaaaaaat
     6841 tcagcaacaa aaactatccg agttacagca atggaggtcg ggcgattaat ccacacacta
     6901 acaccaactt ttttgaaatt tctgatcttg agcctgatgt atatttcgca ttggatacca
     6961 tgaagattgg tggcatgtcc gcaccgattg aattaaaaga tccatatggg gagacagctt
     7021 atcaggtact tttgcttcag gcacgtaccg atccccatag agcaagcctt gaaaaagact
     7081 tttcgcggat caaggaatct gccatgatgt ataaaaaaga agcagagctt gacaattggg
     7141 tgacaaccaa gattcgggat acctttatca ccattgaccc ggaatggttg ggcaagtgcc
     7201 cgggagtaca aaaatgggta aaggccaatt gacattcagt ctttggatca atcaatccat
     7261 taaaagtgag tattgaggct gcttttactt tgccaaataa atattaaggc agtccgggaa
     7321 tgggacagaa atgctgattg atagggtgtt tgccttgtca acctgaagtg agtaggaaaa
     7381 taataatcat tattactgtt cagccaggag tctccggtcg gggcccttac cttttacttc
     7441 acaaatggg
//
LOCUS       DAOQQN010000128         7436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4006_length_7436_cov_5.663460, whole genome shotgun sequence.
ACCESSION   DAOQQN010000128 DAOQQN010000000
VERSION     DAOQQN010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4006_length_7436_cov_5.663460"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLG24_06200"
     CDS             complement(<1..509)
                     /locus_tag="PLG24_06200"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HMZ24514.1"
                     /translation="MKVLYFSLFMTLPYLMQAQYQYPPARMSDQTDNYHGEIIRDPYR
                     WLEDDHAEETKNWVIMENEVTASYFEKIPYIDELTHRLNALYNYPKYSSPFRKGDYYY
                     YYYNSGLQNQSVLYRTKGLNGTPEIVLDPNTFSADGTTKLAGFSLSKNGDYAAVYFSK
                     GGSDWSEAK"
     gene            870..3167
                     /locus_tag="PLG24_06205"
     CDS             870..3167
                     /locus_tag="PLG24_06205"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HMZ24515.1"
                     /translation="MKKWFLLKSLLLTAHFIYAQLNREPVDFVNPLMGSLSTYELSSG
                     NTYPDISMPWGMNSWTPQTAKMGDGWQYYYNANKIRGFKQTHQPSPWINDYGQFSIMA
                     VTGGPKFRERDRMSWFSHKAETATPYYYSVYLADHDVTAEFAPTDRACIFRFTFPEND
                     QSFVVIDAFDKGSYIRINKKERKIIGYTTRNSGGVPDNFKNYFVIQFDQPFDVAYTFR
                     DSILVKDKHDVTANHAGAVIGFKTHRGEKIIARVASSFISYEQASLNLSRELGDKGFE
                     VIKQQGRDIWNKELSRILVEGGSDDETKTFYSCLYRTMLFPRKFYEYDIDNKVMHYSP
                     YNGQVLPGVMYTDNGFWDTFRAVFPFFNLMYPELNSEIMQAMVNVYKESGWLPEWQSP
                     GHRNCMIGSNSASIIADAYIKGIRGFDVETLYQAILKNSNSQGPLQSVGRAGAEEYNE
                     LGYVPYDIGINENAARTLEYAYDDFAVSQLARALKKPDEEVSLFTLRAQNYHNVYDPS
                     TRLMRGRNKDGSFQSPFNPFKWGDAFTEGNSWHYTWSVFHDVNGLAMAMGGRDTMAMM
                     LDSVFSLPPIFDESYYGQVIHEIREMQIMNMGQYAHGNQPIQHMIYLYNYLGQPWKAQ
                     YWLHQVMNRLYNPHPDGYCGDEDNGQTSAWYVFSAMGFYPVCPGTTQYVIGSPLFKRI
                     TLSLSNDKKVQINAPAVNKENVYIQDISLNARPYDKLYLDHFELMKGGKFDFIMTNIP
                     NKTRVYKEAQLPFSMTKWPEPKKKR"
     gene            complement(3218..3745)
                     /locus_tag="PLG24_06210"
     CDS             complement(3218..3745)
                     /locus_tag="PLG24_06210"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMZ24516.1"
                     /translation="MPKVKWIIDPVHSEIEFKVKYLMITNVIGRFDRFDMTVETEEED
                     FNTGKVWFSADVDSLETNDHERNGHLKSPDFFDIAAYPHIIFESGDMKVDGEVGEISG
                     NLTIKNTTKPVTFKVDFGGVTMDMYGTTRAGFSISTEIDRKEFGLRWNSILEGGGMMV
                     GDKVRISGEIQLVKR"
     gene            complement(3939..5579)
                     /locus_tag="PLG24_06215"
     CDS             complement(3939..5579)
                     /locus_tag="PLG24_06215"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HMZ24517.1"
                     /translation="MKRLLVNIAAIFVFSMLIFSCKKEQPIELPDFVGLSATIDDISK
                     ITPTGISLRPKISIHFSSGILSTSASSAIRLEGYSGQLNVSVSDSTLLIQPEGDLAYL
                     EKYRIVVTEKLKSIDDRPLKKAVSYEFYTLLDPSDKLPRIDDAALLDSVQRRTFRYFW
                     EFGHPVSGLARERNSSGDLVTSGGSGFGMMAIPVGIERGFITRRQGLDRATQSVDFLL
                     NKADRFHGAFPHWLSGTTGKVIPFSSNDNGADLVETSFLIAGLLTLREYFDGQQAEEV
                     ELRRDIDSIWQGVEWDWFRRDGKDILYWHWSPDKGWSMNLPISGWNECLITYILAASS
                     PAHGIPPEVYHRGWASNGGIVNNQQAYGINLPLGSGTGGPLFFEHYSFLGIDPRGLKD
                     QYADYWEQVRAHTLINRAYCIANPRHYYGYGPECWGLTAGDVYNGYTASSPANDVSVI
                     GPTAALSSMPYTPEESLDALHFFYYKLGDRLWGKMGFYDGFSLDRAWFAESTLAIDQG
                     PIVIMIENYRTALIWNLLMKAPEIKAGMEKLGFESPHF"
     gene            complement(5623..6960)
                     /locus_tag="PLG24_06220"
     CDS             complement(5623..6960)
                     /locus_tag="PLG24_06220"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HMZ24518.1"
                     /translation="MMCRLIGIFMYLVLTGHYLNAQDYYGAEPEVVKKGLTDDKLLDE
                     IQRVTFRYFWDFADPNSGMARERSNTNTGYGHEVITSGGSGFGVMAIIVAVERGWISR
                     EEGVGRLLKMVNFLRKADSFHGMFPHWWNAETGRVRPFSRKDDGADIVESSFMFQGLI
                     CAMEYFDRDTKEERDLRGKINLTWREAEWDFFTRDGRQVLYWHWSPNNGWAMNFEIRG
                     FNECLITYVLAASSPTYAIDEKVYHNGWAMSNFFKNGKQFYGYTLPLGFDYGGPMFFA
                     HYSFMGLDPRGLKDRYADYWEQNTHHTLINRAYCIDNPKKFKGYGPDCWGLSAGDECG
                     GYSAHSPENDNGYISPTAALASMPYTPEYSMQALRYFYEKMGDKLWTRYGFLDSFNPQ
                     TGCVTPTHLAIDQGPIIVMIENHRSGLLWKLFMKNKDVQRGLTKLGFTFRSEK"
     gene            7229..7408
                     /locus_tag="PLG24_06225"
     CDS             7229..7408
                     /locus_tag="PLG24_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24519.1"
                     /translation="MTKYEKMADRFAKSGLDKDVFEIEAGRKGSTLRYYLQRVKKTRS
                     VHWHWAGRIIYLRES"
BASE COUNT         2038 a         1645 c         1688 g         2065 t
ORIGIN      
        1 atttttgctt cgctccagtc ggagcctccc tttgagaaat ataccgcagc ataatcacca
       61 tttttggaga gtgaaaatcc ggccagcttg gtggtgccat cagcggaaaa tgtgtttggg
      121 tccagaacga tctccggtgt accattcaga ccttttgtcc tgtataatac cgattgattt
      181 tgcaatccgg aattgtaata atagtaataa taatcgccct ttcggaaagg agaggaatat
      241 ttcggatagt tgtaaagtgc atttagccga tgggtcaatt cgtcgatata agggatcttt
      301 tcaaaataac ttgcagtaac ttcattttcc atgattaccc agttcttggt ttcctcagcg
      361 tggtcatctt ccagccaccg gtaaggatcc cggatgattt ctccatgata attgtccgtc
      421 tggtcgctca tcctggcggg tggatattga tactgtgcct gcataaggta tggtaatgtc
      481 atgaataaag aaaaataaag tactttcatc tgaaaaaatt ttggctataa aggtaatctt
      541 tttacaccag aatacataaa tatcctaagt tttcaccagg ggaatagccg gtgaatatca
      601 gtgatacgaa agagatagtg ttgatccaat ataaggattg acctggttga ttatttcagt
      661 tcactctctt ttttttgctt gatcttcaaa taccctattt ggaaaaacag tcaatctgat
      721 tttcctgtta atcaaaggta caatgcgtgc cggtagtgtg ttattaacag gtgtgaaaat
      781 tttagccggt cggattctgt tttcttttta tacattcgta caagtattga cttttcttat
      841 ttttaatttc attgctttag gtttttttca tgaaaaagtg gtttcttctc aaatctttgc
      901 ttctcacggc acattttatt tatgctcaac tcaatagaga gccggttgat tttgtcaatc
      961 ctctcatggg ttccttgtcc acttatgagc tatcttccgg taatacttat ccggatatct
     1021 cgatgccatg gggtatgaac agctggacac cccagactgc caagatggga gatggatggc
     1081 aatattatta caatgccaat aaaataagag ggttcaagca gactcatcaa ccttctccct
     1141 ggatcaatga ctatggtcag ttttcaatca tggctgtaac cggggggccg aagtttagag
     1201 aaagagatcg aatgtcctgg ttttcccaca aggctgaaac tgctactcca tattactata
     1261 gtgtttatct ggccgaccat gatgtgactg cagagtttgc acctacggac cgggcctgta
     1321 tattccgctt tacctttccg gaaaatgatc agtcctttgt ggtcatagat gcttttgata
     1381 aaggttccta cattaggatc aacaagaaag agaggaagat aatcggatat acgacccgca
     1441 atagtggagg tgtgccggat aatttcaaga attattttgt aattcaattc gatcagccat
     1501 ttgatgtcgc atataccttc cgggatagta tattggtgaa ggataagcat gatgtaactg
     1561 ccaatcacgc aggtgctgtt atcggattca agacacatcg tggcgagaag atcatcgcac
     1621 gtgtagcgtc atccttcatc agttatgaac aggcttccct caatctgtcg agggagttgg
     1681 gtgacaaggg cttcgaggtg atcaagcaac aaggccggga tatctggaac aaagagctta
     1741 gtcgtatcct ggttgaggga ggcagtgatg atgagaccaa aactttttat tcctgtctgt
     1801 acaggaccat gttgtttcca cggaagttct atgaatacga tatcgacaac aaagtgatgc
     1861 actatagtcc ttacaacggc caggtattgc cgggcgtcat gtacacagac aatggtttct
     1921 gggatacatt ccgggctgta tttccattct tcaacctgat gtatcctgaa ttgaacagtg
     1981 agatcatgca ggcgatggtc aatgtatata aggaaagtgg ttggttgccg gagtggcaga
     2041 gtccggggca caggaattgc atgatcggta gcaactctgc ttccatcatc gccgatgcat
     2101 acatcaaggg aataagagga tttgatgttg agacgctgta tcaggcgata ttaaaaaaca
     2161 gcaacagtca ggggccgctt cagtctgtag gtcgtgcagg tgcggaagag tacaacgaat
     2221 tgggttatgt gccctatgat attggaatca atgaaaatgc agcacgtacc ctggaatacg
     2281 cctatgatga ttttgctgtg tcacagttgg cgcgcgcgct taagaagccc gatgaggagg
     2341 tttctctatt taccctgcgc gcacagaatt atcacaatgt ctatgatcca tcgaccaggt
     2401 tgatgcgtgg gcgtaataag gatggcagct tccaatcacc cttcaatcca ttcaagtggg
     2461 gtgatgcctt tacggagggt aacagctggc actacacctg gagtgtgttt catgatgtca
     2521 acggactggc gatggcgatg ggcggcaggg ataccatggc tatgatgctt gatagtgtct
     2581 tcagcctgcc accgatattt gacgaaagtt attacggcca ggtaatccac gaaatcaggg
     2641 agatgcagat tatgaacatg ggccagtatg ctcatggaaa ccaacctata cagcacatga
     2701 tctatctgta taattatctt ggacaaccgt ggaaggctca atactggctg catcaggtca
     2761 tgaacaggct ttacaatcca cacccggatg gctattgcgg tgatgaagac aacggccaga
     2821 ccagtgcatg gtatgtgttc agtgctatgg gcttctaccc ggtatgcccc ggtacgactc
     2881 aatatgtcat tggttctccc cttttcaaaa ggattacttt aagccttagc aacgataaaa
     2941 aagtgcagat caatgcgcct gctgtcaata aggagaatgt atatattcag gacatatcac
     3001 tcaatgcccg gccatacgac aagctgtatc ttgatcattt tgaactaatg aaaggaggga
     3061 agtttgactt tatcatgacc aatattccga ataaaaccag ggtttataag gaagcgcaac
     3121 tgccattttc aatgacgaaa tggccggaac caaagaagaa aagataggga ccgcagacgg
     3181 tattaatgga ttaacaaagg aagaccggga cctgtcatta tcttttgaca agttggattt
     3241 ctcctgagat cctgaccttg tcaccgacca tcatcccacc accttccagg atggagttcc
     3301 atcgaaggcc gaattccttg cggtctattt ccgtggatat agagaatccg gctcgagtag
     3361 tgccatacat atccatagtc actccaccga agtccacctt gaaggttaca ggctttgtgg
     3421 tgtttttgat ggtaaggttg ccggagatct cacctacctc accgtcaacc ttcatatcac
     3481 ctgattcgaa aatgatgtgg ggataagctg caatgtcaaa gaaatccgga cttttcaaat
     3541 gcccgttacg ttcatggtca ttggtctcca ggctgtcaac atcagctgaa aaccagacct
     3601 ttccggtgtt aaaatcctct tcttccgttt cgaccgtcat gtcaaaacga tcaaaccggc
     3661 cgatgacatt ggtaatcatt agatatttaa ccttaaattc tatctcacta tgtaccggat
     3721 ctatgatcca ttttactttc ggcatgacgg tatattcatt tatatggtta ttatctaata
     3781 gaaagttcgg actaaaagtt cataaaggta tagaatattt ggatattttc cgtgcttgat
     3841 aaaacgtgct atcttcaaaa attcccgtgt atgatgaggc taattgattg aagggaagtc
     3901 gatttttatt atttcctgct gtaagtttgt ttgaaaccct aaaaatgggg gctctcaaat
     3961 ccaagttttt ccatgcctgc ctttatttcc ggtgccttca tcaagagatt ccaaatcaaa
     4021 gccgtcctat aattttctat cataatcaca atcggcccct gatcgatggc aagggtagac
     4081 tcagcaaacc aggcacgatc gagtgaaaac ccgtcgtaaa accccatttt accccaaagc
     4141 ctgtccccta gcttgtaata aaaaaaatgg agggcatcaa ggctttcttc aggggtgtag
     4201 ggcatggagg ataaggcagc agtggggccg ataacgctga cgtcattggc agggctgctt
     4261 gctgtatatc cgttatatac gtctcctgca gtaaggcccc agcattcagg cccatatccg
     4321 tagtaatgac gggggtttgc tatgcaatat gccctgttga tcagggtgtg tgccctcact
     4381 tgctcccaat agtcggcata ttggtccttc aagccccggg ggtcgatgcc cagaaaagaa
     4441 taatgctcga aaaataacgg acctccggta cctgaaccca atgggagatt aattccgtag
     4501 gcttgttggt tattgacgat gccgccattg ctggcccaac ccctgtggta cacttccggt
     4561 ggaattccgt gtgcaggact tgatgcagca aggatgtatg tgataaggca ttcattccac
     4621 cctgatattg gcagattcat cgaccaaccc ttatcgggac tccaatgcca gtacaggata
     4681 tccttgccat ctcttctaaa ccaatcccat tccacaccct gccatataga gtctatatcg
     4741 cggcgcaatt ccacctcttc agcttgttgc ccgtcaaaat attctctgag agtcagtaat
     4801 ccggcaatta gaaaagaggt ttccaccagg tcagcaccat tgtcattgga gctgaaaggt
     4861 atcaccttac cggtggtgcc gctcagccaa tgtggaaatg ccccatggaa gcgatcggcc
     4921 ttgttcaaca gaaagtctac cgattgggtg gcgcggtcca acccttgccg gcgtgtgatg
     4981 aaaccgcgtt cgatgcccac cgggatggcc atcatcccga agccgcttcc cccacttgtc
     5041 accagatcac cggatgaatt acgttccctg gcaagaccac ttaccgggtg tccgaattcc
     5101 caaaagtatc tgaaagtacg gcgttgtaca ctatccagca acgctgcatc atcaatcctt
     5161 ggaagtttgt ctgacgggtc cagcaaggta tagaattcat aggaaacggc cttcttcaag
     5221 ggcctgtcat ctatgctctt cagtttttca gttactacaa tgcgatactt ttcaagataa
     5281 gcaagatccc cttcaggctg aatcaacagt gtggaatcgg acaccgaaac attcaattgt
     5341 cctgaatatc cttccagccg gattgccgat gatgctgaag tgctcagtat cccggaagag
     5401 aaatgtatgc tgatctttgg tctgagggaa atacctgtgg gagtaatttt ggatatgtca
     5461 tcaatggtgg ctgaaaggcc gacaaagtca ggcagttcga ttggttgttc ctttttacag
     5521 gaaaatatca acatcgagaa gacaaatatt gctgcaatat taacaagcag tcgtttcatg
     5581 gcatcgtaaa ttgaatcgac attgttttaa tcttgaaaac agttattttt ctgatctgaa
     5641 agtaaatcca agtttggtca agccgcgctg tacatcttta tttttcataa aaagcttcca
     5701 caataatccg gaacggtgat tttcgatcat tacaatgata ggtccctgat ctatggccag
     5761 atgggtaggt gtgacgcagc cggtctgagg attgaaggag tcgagaaatc catatcgggt
     5821 ccagagctta tcgcccattt tctcgtaaaa atatcgcaat gcctgcatag aatattcagg
     5881 tgtataaggc atggaggcaa gggccgctgt cggagagata tagccattgt cattttccgg
     5941 tgagtgtgca gaataaccgc cacattcgtc tccggctgac aacccccagc aatccggtcc
     6001 atatcccttg aatttcttcg gattgtcgat gcagtatgca cggttgatca gggtgtgatg
     6061 agtattttgt tcccagtaat ctgcgtacct gtccttcaac ccccgggggt caagtcccat
     6121 aaaggaataa tgagcaaaaa acatgggtcc tccataatca aatccgaggg gcagggtata
     6181 accgtagaac tgcttgccat tcttaaagaa attactcatc gcccatccgt tgtgatacac
     6241 cttttcatcg atggcatagg tgggcgaaga ggcggcaagg acataggtaa tcaggcattc
     6301 attgaagcca cgtatctcga agttcatcgc ccagccgttg ttgggactcc agtgccagta
     6361 caatacctgt ctgccgtcgc gggtgaagaa gtcccattct gcctcgcgcc aggtcagatt
     6421 gatcttccca cggagatcac gctcttcctt agtatcccgg tcgaaatatt ccatggcgca
     6481 gataagtccc tgaaacatga aggaggattc gacgatatcc gcaccatcat cttttctgga
     6541 aaaaggcctt acacgaccgg tttctgcatt ccaccaatga ggaaacattc catggaagct
     6601 atctgccttt ctgagaaaat taaccatctt taatagccgg ccaactccct cctctctgct
     6661 gatccagcct cgttcaacag caacaataat ggccatcaca ccgaacccac ttcccccact
     6721 ggtgatgacc tcatggccat aaccggtatt ggtattgctg cgttctctcg ccatgccgga
     6781 gtttggatcg gcaaaatccc agaagtaacg gaaagtgact ctttggatct catccagcag
     6841 cttgtcatct gtcaatcctt ttttaacaac ttcaggctct gcgccataat agtcctgcgc
     6901 attaaggtaa tggcccgtca ataccaaata catgaaaata ccgatcaaac gacacatcat
     6961 actgataaaa tttctgcaag atagtatttc tttttgaaag aagaggatgg tacagatact
     7021 tacctcatca ttgattttat tgataacagg cagtcgaaaa ttctatagaa ccatatcatt
     7081 gataaaaatc cattccgctg gaagtactat ggttatcgga catggttatg gatcggattt
     7141 aatatgcctg taaagaacgt aaataaccgt ctaagtacat cttttttcat gttatcggtg
     7201 gtcatacctt agaggggaaa taagaaccat gacaaagtat gaaaaaatgg cggaccgatt
     7261 tgcgaagtcc ggtcttgata aggatgtttt tgagatagaa gcagggagaa aaggatcaac
     7321 actgcgatat tatctacaac gagtcaagaa aacaagatcc gtccactggc actgggcagg
     7381 aagaattatt tatttgaggg agtcatgatg gagccgtcag agccgctatg ttgtat
//
LOCUS       DAOQQN010000129         7401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4033_length_7401_cov_6.425538, whole genome shotgun sequence.
ACCESSION   DAOQQN010000129 DAOQQN010000000
VERSION     DAOQQN010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4033_length_7401_cov_6.425538"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLG24_06230"
     CDS             <1..317
                     /locus_tag="PLG24_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24520.1"
                     /translation="ELEDYANYVTRESANNYSEEIKLDINKVWAEKYFGRKEISRAEV
                     DAAAKVSNSGANQLRAMFGLPLQASSVSKDAEIWLHRMALEDAIKLGLKKYGKSSGCA
                     KP"
     gene            411..1256
                     /locus_tag="PLG24_06235"
     CDS             411..1256
                     /locus_tag="PLG24_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24521.1"
                     /translation="MKFIKIIIVSFIPFLLTSQNLPSDSKVLEDVKKYHGKIATAKVQ
                     NEWKLEKEPGYTFSNMAKRVVAATTVKENNITKNLIGLAIYVRGGSGEKWNFSRYFVT
                     SSQITGLAPLNEQQIKDQTIQLLRSNPGRVFVNFSDIAWVYDLSFPEGLKSETDRTGD
                     IIYKAQIEYEQKFISSYPFEGGLRRYKSPLEIYVRPIDGVLKVAVASLGYSDPIKETL
                     MTEKQYNALIVLGQKPFDELYGTQGPSVSGNTKSEGDNEAGKSKSNNNSEKKGIKMPV
                     IKLKN"
     gene            1307..3406
                     /locus_tag="PLG24_06240"
     CDS             1307..3406
                     /locus_tag="PLG24_06240"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ24522.1"
                     /translation="MRSIIICAFFNILICSLYAQGEYMSFKNKVASLKKVVGKGNNVA
                     NELMLNQAVLLKDSIYQSGFFDLSGDMLMVIGRLNQQLGKMGNAELYYKEGINKGQQY
                     NDSIWVAKAWDRLGDFYSLEERNYLSLECHLKALKLKEKYDSNQQTIPETYHRIARSY
                     IQTGSLATAEKYLDQALKLKKRLNDTLRLGIITTLYADIYRLKGNYALAEQYYLKDLP
                     KRLNQNNYEGLTISFLGLAQNYQNWGKYRDAEIYYLKALQSAETINRHRNIGLILLEL
                     GNLYQSMGQPDRAKAVFKRAIESCTQVDSRTYQLNAYHNLYLMYKKEGNLPKAMNYLE
                     LYTAVQDTSAKEYLNMKIEDLKAGLELHEKERKITELDSENKKAKFIRNILIIGICLL
                     LLLSVFLVVLYRLRNKANDALNKEQKHTKSLLAEKEKLLTDLHHSNVQLMHAEKMASL
                     GVMTAGIAHELNNPISSIHASVEALMMDFKDLEPLFNLLRESKNPDNTDNHHSHYNDL
                     NLNKVDIAYLSKEVESLLKTIKNGSQRTAEIVQGLKNFSRDSGDRLQPYQVEEGIETA
                     LTLLNHKINPNIKIITSYRFKGTILCQISKINQVLLNIIDNAIQAVEKKGVIDIETYQ
                     KDAFCIIKIKDTGPGMDTETQKKIFEPFFTTKDVGKGTGLGLSISYAIIQQHKGELSV
                     ESHPGKETVFTIKLPIH"
     gene            3443..4225
                     /locus_tag="PLG24_06245"
     CDS             3443..4225
                     /locus_tag="PLG24_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24523.1"
                     /translation="MKGYKGTFLNLILTALIFMVDSKVRQESPSMVNLHLPIHYLIVY
                     SILILFERRANLNILSIEFKQISHQQNKVWERFRKSWYFGIQILMSIVWRTYFRTVLI
                     LFPLLGYIYKNFDITYQTAIWPLKLPYWVVCGWIGVREFRMVAAILEPPNHHVSLPTE
                     WISRVIQVVLNTLFLSHLTIMMNDYIDVSKDLFTALLQITPFLLIVFTFFYIPIRFVE
                     VVSDFIDLRSKWQTIIFWISTFFLMLTVVAPKITGQIIRFDY"
     gene            4606..5619
                     /locus_tag="PLG24_06250"
     CDS             4606..5619
                     /locus_tag="PLG24_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMZ24524.1"
                     /translation="MSELYTILYLDDEEDNLTSFRAVFRRFYHVYTANNADEAMEILE
                     KSNINLVISDQRMPGITGVQFLEKVYQSYPDTIRMILTGYSDMQAIIDAINKGRIYYY
                     ITKPWKFDTLKIILDNALESYALKLKNTKLEDEKKSLIIKTLRQEKEQIFFQHEILKN
                     QINPHFLFNCLNTLASLIGDNQEQAITFTTRFSRLYRQVLEHGEQVLISLDKEVDLIK
                     NYIFLQQIRFGKCLDCKMEITDYSYSIPTFALQLLVENAIKHNVISETHPLQINLDQD
                     MDIIKVSNKIQNKNENKSSSGIGLKNLRQRYKLHGVSNLEIIKDDGYFIVVLPLLPLI
                     NDH"
     gene            5621..6379
                     /locus_tag="PLG24_06255"
     CDS             5621..6379
                     /locus_tag="PLG24_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HMZ24525.1"
                     /translation="MDLRILIIEDEKPAQEYLGRLIMRLEPNATILTKIDSIKSSVEF
                     LRNNSVDLIFMDIQLSDGISFRIFDQVEVVSPIIFTTAYDSYAINAFKVNSIDYLLKP
                     VDENELRISLDKFKKLSRPVTNMDMELLINALQSKDRLYQERFVVHRGERMMSVPVDQ
                     IAYFEGEDRYVYLVKKDGSKFIVDYRLSDLEKILNPEIFFRLNRSFIAHFDSIHSMIK
                     VSKSRIKIELLPVTKREIIISSESSQEFKLWLNR"
     gene            6448..7071
                     /locus_tag="PLG24_06260"
     CDS             6448..7071
                     /locus_tag="PLG24_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24526.1"
                     /translation="MKSTSKDPDSFIPEGYHQIRRVSGQLNGDTLTDYVFIIESNDLV
                     SNLKEEGHDQKPRILFILFGDKEYGFKLAAQSNKSVMLSDDGGVFGDPLQELFIRNKV
                     IHVNYFGGSTEKWAYTYKWRYQHGDWLLIGATYTSMSPYNNMLEKYDFNLNTGDMEHT
                     IKPFLEEDEDVPTVTPKTKRYKIKKNKLYSLNSFQVGVNLIYKDINF"
BASE COUNT         2571 a         1213 c         1370 g         2247 t
ORIGIN      
        1 atgaattaga agattacgct aattatgtta caagagaatc agccaataat tattcggaag
       61 agattaaact tgacatcaat aaggtatggg ctgaaaaata ctttggtcgc aaggagatat
      121 ccagagcaga ggtagatgca gcagctaaag tatccaatag cggtgcaaat caattaagag
      181 ctatgtttgg attgcccctg caggcaagta gtgtttcaaa agatgctgaa atctggctgc
      241 atagaatggc cctggaagat gctataaagt tgggactaaa aaaatatggc aaaagtagtg
      301 ggtgcgccaa accttgaaat aaacactgac aataattaat gtaaagtaaa tgtaaaacca
      361 ggtttgcttt tattcaaatt atattaaaaa tcataaataa aaattgaagt atgaaattca
      421 tcaaaataat aatcgtatca ttcattccct ttcttttaac atcacaaaat ttaccttctg
      481 actccaaagt tttggaagat gtaaaaaaat atcatggcaa aattgcaacg gctaaagtcc
      541 agaacgaatg gaaattggaa aaagaacccg gatatacatt tagcaacatg gcaaagagag
      601 ttgttgccgc tacgactgta aaagaaaata atataactaa aaatctaata ggccttgcaa
      661 tctatgtgcg aggtggaagc ggtgaaaaat ggaacttttc aaggtatttt gtgacttcct
      721 cacaaattac tggacttgca ccacttaatg aacaacagat caaagaccaa acgatacagc
      781 tgctacgttc aaatcccggc cgggtttttg tgaattttag cgatatcgca tgggtttacg
      841 atttatcttt tcctgaaggt cttaaaagtg aaactgacag aaccggagac attatatata
      901 aagcccagat cgaatatgaa caaaaattta taagttctta tccttttgaa ggaggattgc
      961 gtagatataa gtctccactg gaaatttacg tccggccgat tgatggggtt ttaaaagtag
     1021 ctgtggcatc acttggttac agtgacccca ttaaagaaac actaatgaca gagaaacaat
     1081 acaatgcctt aatcgttctt gggcagaaac catttgatga attgtatgga actcaggggc
     1141 catctgtatc cggcaataca aaaagcgagg gagataatga agccggaaaa tccaaatcca
     1201 ataataattc agaaaaaaag ggtataaaaa tgccagttat taaattaaag aattgatctg
     1261 gatttttttt aataagttta aatttgttta cgacatcaat cggtaaatgc gaagtattat
     1321 tatatgtgct ttctttaata ttcttatttg cagcttgtat gctcagggag agtatatgtc
     1381 ttttaaaaat aaagtagcta gtttaaaaaa ggttgtcggc aagggcaata atgttgccaa
     1441 tgaattgatg ttaaatcagg cagtgttatt aaaggatagt atttatcagt ccggtttttt
     1501 tgatttgtcc ggagatatgc tgatggtcat tggaagacta aatcagcaac tgggtaaaat
     1561 gggcaatgct gaactatatt acaaagaagg aataaataag ggtcaacaat ataatgacag
     1621 tatatgggta gctaaagcat gggacagatt aggtgatttc tattccttag aagaaagaaa
     1681 ttacctttct ttagaatgcc atcttaaagc tttaaagctg aaagaaaagt atgattcaaa
     1741 ccaacaaacc attcctgaaa cctatcatcg tattgcccga tcctatatcc agacaggatc
     1801 actggcaact gctgaaaaat accttgacca ggccctgaaa ttaaagaaac gcttaaatga
     1861 tacactcaga cttggcatca ttactacatt atatgcagac atataccgat taaaagggaa
     1921 ttatgcattg gctgaacagt attatttaaa agatttacct aagcgattga atcaaaataa
     1981 ttacgaagga cttaccatta gttttctagg attggctcaa aactatcaga attggggcaa
     2041 atacagggat gctgaaatct attacctcaa agcacttcaa tcagcagaaa ctattaacag
     2101 acaccgtaac attggtttaa tattattaga attaggcaat ctctaccaat caatgggaca
     2161 acctgatcgt gcaaaagccg tgtttaaaag agccattgaa tcttgtacac aggtagattc
     2221 aagaacatat caactcaatg cttatcacaa cctctattta atgtataaaa aagaaggaaa
     2281 tctgccaaaa gcaatgaatt accttgaatt atatacagca gttcaggata catccgccaa
     2341 agagtacctt aatatgaaaa ttgaggactt gaaagcagga cttgagttac atgagaagga
     2401 aagaaaaata actgaattgg atagtgaaaa taagaaagca aaatttatca gaaatattct
     2461 gattattgga atttgtctgt tacttctttt atctgttttt ttagtagtac tttacagatt
     2521 aagaaacaaa gccaatgacg cactcaataa agagcaaaaa catactaaat cattgctggc
     2581 agaaaaggaa aagttgctta cagatctgca tcactccaat gttcagctga tgcatgcgga
     2641 aaaaatggca tcattggggg taatgactgc aggtatagca catgaattaa ataatcctat
     2701 cagttcaata catgcaagtg tagaagccct gatgatggat ttcaaagacc ttgaacctct
     2761 ttttaatcta ttaagagaga gtaagaatcc agacaatacg gataaccatc attcacatta
     2821 taatgacctt aacctaaata aagtagatat tgcctatcta tctaaagagg ttgaatcact
     2881 tttaaaaact atcaaaaacg gatctcaaag aactgcagaa atcgtacaag ggttaaagaa
     2941 cttttcgagg gattccgggg accggctaca accctatcag gtagaagaag gaattgaaac
     3001 agcgttaact ttgctcaatc acaagataaa tccgaacatc aagattatta ccagttatcg
     3061 gtttaagggt acaatccttt gtcagattag caagatcaat caggtattgc ttaacattat
     3121 tgacaatgca atacaggctg tggagaaaaa aggagttata gatatagaaa cctatcaaaa
     3181 agacgccttt tgtatcatta aaattaaaga tacgggcccg ggtatggata ctgaaactca
     3241 aaagaaaata ttcgaaccat tttttacaac taaagatgta ggtaaaggca ccggattggg
     3301 attatccata agttatgcaa ttattcaaca gcacaaaggg gaattatcag tagaatctca
     3361 tccgggtaag gaaaccgtat ttactatcaa gttgccaatt cattgatgcg cctggaatat
     3421 attcatacat tgaccggtat aaatgaaagg atataaaggc acctttttaa atctaatcct
     3481 gacggcactg atttttatgg ttgattctaa ggtgaggcag gaaagtcctt ctatggtgaa
     3541 tttgcattta cctatacatt atttgatagt ttacagcatt cttattctat tcgaaagaag
     3601 agcaaatctg aacattctaa gtatcgaatt taaacagatc agccatcaac aaaataaggt
     3661 ttgggagagg ttcaggaaaa gttggtattt tggtattcag atactgatgt ctattgtttg
     3721 gagaacttac ttcagaacgg ttctgattct ttttccattg cttggctata tctataaaaa
     3781 ttttgatata acttaccaaa ctgcaatctg gccactaaaa ttaccttatt gggtcgtttg
     3841 tggctggatt ggagtgagag agttccgtat ggtagctgct attcttgaac cgcccaatca
     3901 tcatgtttca ttgcccaccg aatggatttc acgtgtaatt caggttgttc taaatacatt
     3961 atttttaagc caccttacaa taatgatgaa tgattatatt gacgtatcaa aagatctttt
     4021 taccgcccta ttacaaatca ccccattttt attgatcgta tttactttct tctatattcc
     4081 aattcggttt gtggaagtag tgtcagattt cattgatctt cgttcaaaat ggcagacaat
     4141 aatattttgg atcagtactt ttttcctaat gttgactgta gttgcgccca aaattaccgg
     4201 tcagattatc aggtttgact actaaataat cccatctgac atcaaacgaa ttttatttcg
     4261 aatcacgctc atctatcttt atatccaatt ataattaagg ccatggggat actattaggt
     4321 tttgtactta tctgtttgat tctatatgca gttctggtgt ctcatgcatt atattttgtc
     4381 gtaaaattaa tactcataaa tctgctgcat atcgttgtat cgaaaaaact gatttgggtt
     4441 ttagtaaatg tatttttatg ggcattggcg acccggttta tttatttatt actattagac
     4501 tcacgaggag gcacaccgaa tattgaagga gtttggaaat tttatataat cttaaacacc
     4561 atcctgtttt taatagtaat tttgcttgga tataaatcca aataaatgtc agaactatat
     4621 actattttat atttggatga tgaagaagat aatctaacat catttagagc tgtatttaga
     4681 agattctatc atgtgtacac agcaaataat gctgatgaag ctatggaaat attggaaaaa
     4741 tccaatatca acttagtcat tagtgatcaa aggatgccgg gtattacagg cgtacaattt
     4801 ctggaaaaag tgtatcaatc atatcccgat acaattagaa tgatcctgac gggatatagt
     4861 gatatgcagg caataataga cgctatcaat aaaggtagaa tttattatta tataaccaaa
     4921 ccttggaaat ttgatacact taaaattata cttgataatg ctttggagag ctatgcctta
     4981 aagctaaaaa acacaaaatt agaagatgag aaaaaatcat taatcattaa aacactaaga
     5041 caggagaaag aacaaatatt tttccagcat gaaattctga aaaaccagat aaaccctcac
     5101 tttctcttca attgtcttaa tacattggct tctttaattg gtgacaatca ggagcaggct
     5161 attacattta ctaccagatt ttccagatta tacagacaag tattagagca tggagagcaa
     5221 gttcttattt cacttgataa ggaggtagac ctcataaaga attatatatt ccttcagcaa
     5281 ataaggtttg gaaagtgcct tgattgtaag atggaaataa cagattattc ctattcaatt
     5341 ccgacatttg cccttcaatt gcttgttgag aacgcgataa aacacaatgt catctctgaa
     5401 actcatccct tacaaatcaa cctggatcag gatatggata tcattaaagt ttcaaataaa
     5461 attcaaaaca aaaatgaaaa caaatcttct tccgggatag gattgaaaaa tctgagacaa
     5521 aggtacaagt tgcatggtgt gtcaaacctg gaaattatta aggacgacgg atactttatt
     5581 gtagttcttc ctctgcttcc actcataaat gatcattaat atggatttaa gaattctaat
     5641 cattgaagat gagaagcctg cacaggaata cctggggagg cttattatgc gactcgagcc
     5701 caatgcaact attttgacaa aaatcgacag tataaaatca tccgttgaat ttctcagaaa
     5761 caacagtgtt gatctgattt ttatggatat ccagctttca gatggcataa gtttcagaat
     5821 ttttgatcag gttgaagttg tatcgccaat catatttact acagcttatg attcctatgc
     5881 aataaatgcc tttaaagtta acagtataga ttacttgctt aaaccggttg atgaaaatga
     5941 attacgaata agtctggaca aatttaaaaa actatcccgc cctgtgacta atatggatat
     6001 ggaacttctg ataaacgcat tgcagtcaaa agaccgatta tatcaggaaa gatttgtggt
     6061 acacagagga gagagaatga tgagtgtgcc ggtagatcaa attgcttatt tcgaaggtga
     6121 agaccggtat gtttacttag ttaagaagga tgggtcaaaa tttattgtag attatagact
     6181 ttcagacctt gaaaagattc ttaatcctga aatattcttt aggctaaatc gcagttttat
     6241 cgcccacttt gactcaatcc attcgatgat aaaggtgagt aaaagcagaa taaaaataga
     6301 attgttgccg gtgacaaaaa gggaaattat aatcagcagt gaaagtagtc aggaattcaa
     6361 actctggttg aacaggtaat tgtttttaag atgtatttct ttagcctttg catactatta
     6421 atccatttca cgtttcaata tcctcatttg aaatctacgt cgaaagatcc ggattctttt
     6481 atccctgaag gttatcatca gatacgcagg gtttcgggtc aacttaatgg agacaccctg
     6541 acagactatg tatttattat tgaatcaaat gacctcgttt ctaatttaaa agaagagggt
     6601 cacgatcaaa agcctcgtat tcttttcata ctatttggtg ataaagaata tggatttaaa
     6661 ctggctgctc agtccaataa gtcggtcatg ttatctgatg atggaggggt gtttggtgat
     6721 ccgctccagg aattatttat aagaaataag gtcatccatg ttaattattt tgggggaagt
     6781 acagaaaaat gggcgtatac ttataaatgg cgatatcagc atggtgattg gttgctcatt
     6841 ggagccactt atacatctat gtcaccttac aataacatgc tggaaaaata tgatttcaac
     6901 ttaaataccg gagatatgga acataccatc aaaccatttc tggaagaaga tgaagatgtg
     6961 cccactgtta cacccaaaac taaaagatat aaaatcaaga aaaacaagct ctattcttta
     7021 aactcatttc aggttggagt taatctgatt tataaggata taaattttta acccaagttg
     7081 cacttatgcg gctgaaattc tgcacagagt ggaagtttcc ggagggtgat gttctatttt
     7141 gtgtactaca gattttttga caaacggata tttctaataa cctataacat cctgtatttt
     7201 ttgaaaaccc tgtgacggaa tggagttctc cctgacttca attagctggt ccgccatatt
     7261 agtgattctt gttgtaacta tcttctgtat tcatgctatc ccatgggtct ctatccatga
     7321 gggattgata gagggccaat gtgattttct ccccagccaa gcgatttcag ataaccatca
     7381 atgccaagct cctttaccgc c
//
LOCUS       DAOQQN010000130         7396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4035_length_7396_cov_5.133633, whole genome shotgun sequence.
ACCESSION   DAOQQN010000130 DAOQQN010000000
VERSION     DAOQQN010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4035_length_7396_cov_5.133633"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PLG24_06265"
     CDS             <1..836
                     /locus_tag="PLG24_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HMZ24527.1"
                     /translation="PSYIHLDRYDIRRIGNDLNFPGIKKVRRIGHSMEFEQIKEYVAG
                     DDWRSVNWKATSKTAKLMVNQYMEEKAQPVYCLIDTGRMMKMPFDGLTLLDHCINSVL
                     VLSHVVIGNQDKAGLATFSRKLENFLPAERRTGQMHLILEKLYRIRTDYKESDFGRLY
                     NDIRRLINQRSLLLLYTNFDTIDALRRQLKYLRGIAKGHVLLIIFFKNTEMEAIKYEE
                     ALDIREIYDRAVVEKFNFEQRLIVNELRKYGIYSILTEPRYLTVDLINKYLEVKARGL
                     I"
     gene            complement(854..1282)
                     /locus_tag="PLG24_06270"
     CDS             complement(854..1282)
                     /locus_tag="PLG24_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HMZ24528.1"
                     /translation="MYWYLEVFSRYADFSGRSRRKEYWMFILFNMLFLFGIFAIMTAS
                     LAIFGDSRIVTLLLGIVMIIYVLIVFIPSLAVTVRRLHDTGKSGWYILLNLIPYVGGL
                     IIFIFTVMDSEPGENKWGPNPKEDYLYDSEIDEIGMKDEY"
     gene            complement(1517..3436)
                     /gene="dnaK"
                     /locus_tag="PLG24_06275"
     CDS             complement(1517..3436)
                     /gene="dnaK"
                     /locus_tag="PLG24_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HMZ24529.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGNDPVVIPNDEGARTTPSVVAFMD
                     NGERKIGAPAKRQAITNPQRTIASIKRFMGHRFSEVGADVEHSAYKVIKGDNDTVRVE
                     IDGRFYTPQEISAMILQKMKKTAEEFLGQEVTEAVVTVPAYFNDSQRQATKEAGEIAG
                     LNIKRIINEPTAAALAYGLDKKHKDSIVAVYDLGGGTFDISILELGDGVFEVKSTNGD
                     TQLGGDDFDKKIIDWLADGFKAQENIDLRKDPMALQRLKEAAEKAKIELSSGNETEIN
                     LPYVTAIDGVPKHLVMKLNRSKFEQLTDDLVSRSIEPCRQALKDAGLTTNDIDEVILV
                     GGSTRIPKIQEAVEQFFGKKPNKGVNPDEVVAIGAAIQGGVLSGDVKDVLLLDVTPLS
                     MGIETMGGVYDVVIEANSTIPTKKSKIYSTAADNQPSVEIHILQGERPMAKDNRSIGR
                     FILGDIPAAPRGTPQIEVTFDIDANGILQVTSKDKGTGKQQSIRIEASTGLSKEEIER
                     MRTEAAANADADKKAKERIEKLNGADSMIFQSEKQLKEYGDKISAGNKTAIEAALNDL
                     RDAHKTENFDKIDSASAALNNAWQAASQEMYQAGQTGADPGAGAGTESNHSHNNSNGA
                     SEDVTDVEFEEVDKN"
     assembly_gap    4114..4213
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4763..5800)
                     /locus_tag="PLG24_06280"
     CDS             complement(4763..5800)
                     /locus_tag="PLG24_06280"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HMZ24530.1"
                     /translation="MRQIVNYSLFCVFSISVLFFSSCNNELELIGDKVEIPVVYGTIS
                     TTGDFVFIRLERAFGDNKISPGILSKNPDSLYYKDAKVTISLPEENFSIDLEKTDAES
                     LGIKRDTGVFANTPNYIYVAPNDQFVFKPGAEYQLTIEVKGKTYTAKTTLVNTIEIIL
                     PTHTSQVAWIPRTPELQSQYPRVLYEPKGENRSADPAILDLHIRFNYSEKDSRKGNTY
                     EKKSFLIPVTGSIITDANTDFRYSPNRIFEYLRDHMEKDPAFDRFFTSFDFVITGGGR
                     EFLNFTEALAANAGITGTQDFPLYSNIEGGQGIFSSKNTMEFKGYNISSASLDSLAFS
                     RYTIGLNFRKI"
     gene            5904..6719
                     /gene="panB"
                     /locus_tag="PLG24_06285"
     CDS             5904..6719
                     /gene="panB"
                     /locus_tag="PLG24_06285"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HMZ24531.1"
                     /translation="MSIQKEIKRVTTHIMQEMKNNGEKISMLTGYDYSMARIIDDAGV
                     DIILVGDSASNVMAGHETTLPITLDQMIYHASSVIRAVKRSFVVVDLPFGSYQGDSKK
                     ALNSAIRIMKESGAHGVKLEGGREVGESVKRILTAGIPVMGHLGLTPQSIYKFGTYSV
                     RAKEEEEAQRLVEDSQYLESLGCFSIVLEKIPASLAEKVATTLTIPVIGIGAGGGVDG
                     QVLVTHDLLGINKEFKPRFLRLYEDLYSRMFDAFSAYIRDVKSGEFPNKSEQY"
     gene            6765..>7396
                     /locus_tag="PLG24_06290"
     CDS             6765..>7396
                     /locus_tag="PLG24_06290"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HMZ24532.1"
                     /translation="MKLKSLKRPLIVIGAMIIVAAFIFKGSIVPNVTSAAETKFIFVR
                     KGQSLKSLADSLEAGGLVKNGSAILKTAGIMGFKDSDLVPGRYKAEKGWNNIRLLDFL
                     SRGKQTPLNLVINPMRKVENVAAFLGSKMEHDSLDFIIALSDSTFLDSFEVNTANAMT
                     LFIPNTYQVYWNAKPKDFLRRMKKETDRFWAKAGRMDKLKALGMTKEQVYT"
BASE COUNT         2013 a         1485 c         1558 g         2240 t
ORIGIN      
        1 acccttcata tatccatctg gacaggtacg acatcaggag gataggcaat gatcttaatt
       61 ttcccggaat aaagaaggtg aggaggatag gccatagtat ggagtttgag cagatcaagg
      121 aatatgtggc tggagatgat tggcggtcag tcaactggaa ggctacttcc aagacagcca
      181 aactcatggt caatcaatat atggaagaga aggcgcaacc ggtatattgt ctgattgata
      241 cgggtaggat gatgaaaatg ccgtttgatg gacttactct gctggatcac tgtatcaata
      301 gcgtcttagt actaagccat gttgtaatag gtaatcagga caaggccgga ttagcgactt
      361 ttagcaggaa attggaaaat ttcctgccgg cagaacgaag aacagggcag atgcatttaa
      421 ttctggagaa gctctatcgg attagaactg attataagga gtctgatttt ggaaggttgt
      481 acaatgatat tcggcgtctg atcaatcaga gaagtttgct tttgttgtat accaattttg
      541 atacgataga tgcacttcgg cgccagttga agtatttgcg cggtattgcc aaagggcatg
      601 ttttgctgat catttttttt aaaaatacgg agatggaagc aatcaagtat gaagaagcac
      661 ttgatatccg tgaaatttat gacagggcag tggttgaaaa gttcaatttc gaacagagac
      721 tgattgtcaa cgaattgcgt aaatatggca tttattccat actaacagag cccaggtacc
      781 taacagttga cttgatcaac aaatacttgg aagtaaaggc cagaggactg atataaattg
      841 agcccaacaa tggtcaatac tcatccttca ttccgatttc atcaatttca gaatcataca
      901 ggtagtcttc ttttgggtta ggtccccatt tattctcacc aggttcgctg tccataacag
      961 taaaaataaa tattatcaat ccacctacat aaggaataag gttcagaaga atgtaccatc
     1021 cacttttacc ggtatcatga aggcgtctta cggtcacggc cagagaaggt ataaaaacga
     1081 ttaaaacata tattatcatc acgatgccca aaaggagggt aacgattcta ctgtcaccga
     1141 aaatggctaa tgaagcagtc ataatagcaa aaatgccgaa taaaaaaagc atgttgaata
     1201 gtatgaacat ccaatattcc tttcttctgg atctgcctga aaagtcagcg tatcttgaga
     1261 atacttccaa ataccaatac ataacaaata tttttcttca aaaataataa ataaatcatt
     1321 aacaattctc cttatcgcca taaaatataa aaaaaggccg gactgataag cccggccttt
     1381 ttttaattta tggggtataa tacggattgc aattggataa catttattct atcctgataa
     1441 tttctctccg tcaatatcct aattgaaagg ttcagtttca gcctgttaag taggggctga
     1501 tgacctttaa aagggattaa tttttatcca cttcttcaaa ttctacgtct gtaacgtctt
     1561 cacttgcacc attactgttg ttgtgagaat ggttactttc cgtaccggca cctgctccgg
     1621 gatcagctcc ggtttgaccg gcttggtaca tttcctgact tgcagcttgc caggcattgt
     1681 tcagtgcggc acttgcagaa tctatcttgt caaaattttc tgttttgtga gcgtctctga
     1741 gatcattcag agctgcttca atagccgttt tgttaccggc agagattttg tctccatatt
     1801 ccttaagctg tttttcagac tggaagatca ttgagtctgc tccattgagt ttctctattc
     1861 tctctttagc cttcttatct gcatcagcat tagcagcagc ttctgtacgc attctttcaa
     1921 tctcttcctt tgacaatcca gtagaagctt cgatacggat gctttgttgc ttaccggtac
     1981 ctttatcctt tgaggttacc tggaggatac cgttggcatc gatatcaaag gttacttcaa
     2041 tctgaggagt gcctctaggg gcagctggaa tatcaccaag gataaacctt ccgatagatc
     2101 tgttatcctt tgccattggc ctttctccct ggagaatgtg gatttctaca gagggttgat
     2161 tgtctgctgc ggttgagtat attttggatt tcttggtagg aatggttgag ttggcttcaa
     2221 taaccacatc atatactcct cccattgtct cgattcccat ggataagggc gttacatcga
     2281 gtagcaatac atccttaaca tctccactca agacaccccc ctgaattgca gccccgattg
     2341 ctacaacctc gtccggattg actcctttat tcggtttttt accgaaaaat tgctcaacag
     2401 cctcctggat cttaggaatt ctggttgatc caccgacaag aatcacttcg tctatatcat
     2461 tggtagttag acctgcatcc ttcaatgctt gacggcacgg ttctatactt ctgcttacca
     2521 gatcatcagt caattgttcg aatttagatc tgttgagttt cattacaagg tgtttaggaa
     2581 caccatcaat tgctgtaaca taaggcaggt tgatttctgt ctcattcccg gaggataatt
     2641 cgattttggc cttttctgca gcttccttca agcgctgaag tgccataggg tcttttctaa
     2701 ggtcaatatt ttcctgcgcc ttaaaaccgt cagctaacca atcgataatt tttttatcga
     2761 aatcatcacc tcccaattga gtatctccat tggttgattt tacttcaaat acaccatcgc
     2821 ccagttcaag gatggatata tcgaaagtac ctcccccaag gtcatacaca gccacaatag
     2881 aatccttatg tttcttatca aggccgtatg ccaatgcagc ggcagtaggt tcattgatga
     2941 ttcgcttaat gttaaggcct gcaatttcgc ccgcttcttt ggtagcctgg cgctgtgagt
     3001 cattgaaata agcaggtaca gtaaccactg cttctgtaac ttcctggccc aggaattctt
     3061 cagctgtctt tttcattttc tgaagaatca tggctgaaat ttcctgtggg gtataaaatc
     3121 tgccatcaat ttctactcgc actgtgtcat tgtctccttt tattactttg taggcggaat
     3181 gttctacatc cgcacctact tcagagaatc tatgacccat gaatctttta atggaggcaa
     3241 tagtcctctg agggttggtg attgcctgcc ttttagctgg tgcaccgatt tttctttctc
     3301 cattgtccat aaaggcaact actgaaggag tagtccttgc accttcgtcg tttggtatta
     3361 cgacagggtc gttaccttcc attacggaaa cacaggagtt ggttgttcct aagtcaattc
     3421 ctattatttt tcccatatct attgtttttg aattataaat ttaatccgat atagtcaact
     3481 agtgtgccat tcgaatctga ccgacaaaat gtcatattaa gtggtgagcg aatgagggaa
     3541 ggtgccaaat ttgagtggaa tgtttagaat atgtgaaata ttggcagttg gaatataggg
     3601 tggtaaatgg gaatgaggtc cgcaacgaag taggtagatg attaataact tcccttactg
     3661 tgtgaggagg cattttcggc gattgcaatt tgaaatacgc gttgtgatga acaaaaattt
     3721 aaccagtgtt ttgcaggagg tgcatcagta gggagttcat gactattatt ttcctaccta
     3781 cttcgatatt tcctctttca ggtctctgcc gacgatgtga aaatccgctg attgcatggt
     3841 ccgcagcgaa gtaggtagat gattaataac ttcccttact gtgtgaagtg gcatttccgg
     3901 cgatcgtatt ttgaaatacg cgttgtggtg aacaaaagtt taacccttga ttctcagaat
     3961 gcgcgtcagt agggagttca tagctattac tttcctacct acttcgattt ttcattttcc
     4021 aaattgctgc cgacgaggta aaaatccgct gattgcacgg tccgcagcga agtaggtaga
     4081 tgattaataa cttcccttac tgtgtgaagt ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnatttccg gcgatcgcaa tttgaaatgt acgttgtgat gaacaaaaat
     4261 ttaaccagtg ttttgcagga ggtgcatcaa tagggagttc atgactatta ctttcctacc
     4321 tacttcgatt tttccttttc caaattgctg ccgacgatgt gaaaccctac tgatttcacg
     4381 gtccgcaacg aagtaggtag atgattaata gcttccctta ctgtgtgaag cggcatatcc
     4441 ggcgatcgca atttgaaata cgcgttgtga tgaacaaaag tttaaccagt attttgcagg
     4501 aagcgcgtca gtagagagtt catagctatt actttcctac ctacttcgat ttttccccgc
     4561 tcatatcaac cgttacgcca tatcctttgg cttctatttg tttattccgg ctatgtcacc
     4621 ctaagataga tgtaaaggcg ctttttgaaa caaaagctga atttttgcca agatcctcaa
     4681 tattaaacag caatgagaat taactccata aaagtaaatt tcatggcatt gaattaaggg
     4741 accttcagat catcttaagt ttttagatct ttctgaaatt caggccgatt gtatatctgc
     4801 tgaatgctag tgaatcaaga gaagctgagc ttatattata gcctttgaat tccatggtgt
     4861 tcttggaaga aaagatcccc tgtccgcctt ctatattcga ataaagcgga aaatcctggg
     4921 tgccggtgat accggcatta gctgccaatg cctctgtaaa gttgagaaat tccctgccac
     4981 cacctgtaat cacgaagtca aatgaggtaa agaacctgtc aaatgccggg tctttttcca
     5041 tatgatctct aaggtattca aagattctgt taggcgaata cctgaagtca gtattggcat
     5101 ctgttatgat ggatccggtc accggtatca agaaactctt tttttcatat gtatttcctt
     5161 tcctgctgtc tttctctgaa taattaaacc tgatatgcaa gtctaggatg gccggatctg
     5221 cactcctgtt ttctcctttt ggttcataaa gtactctggg atactggctt tgaagttccg
     5281 gagttcgagg tatccatgcc acctgactgg tgtgagtcgg gaggatgatc tctatggtat
     5341 tgacaagcgt ggttttagct gtgtaggttt tgcctttgac ctcaatcgtt agttgatact
     5401 cagcccccgg tttgaacaca aattggtcgt ttggagcgac atatatatag ttgggtgtat
     5461 tggcaaaaac tccggtgtct ctttttatgc ccagggattc agcatcagtc ttctctaaat
     5521 cgattgaaaa attctcctca ggcaggctga ttgttacttt ggcatcttta taataaaggc
     5581 tatccgggtt ttttgataaa atgccgggac tgattttatt atcaccaaaa gcacgctcca
     5641 gtctgataaa aacaaaatcg ccggtggtgg aaatagttcc atacaccaca ggtatttcga
     5701 ctttgtcacc gatcaattcc aactcattgt tgcatgaact gaaaaataat acggaaatag
     5761 agaagacaca aaagagggaa taatttacta tttgacgcat atttgtattc aattaattta
     5821 gccggatgat taatcttcaa ataaaattcc tgcgaaatta ggcatttttt taacttgcag
     5881 cacatttttt aacattcgtt tttatgtcaa ttcaaaagga aatcaagcgg gttactaccc
     5941 acatcatgca ggagatgaag aacaatgggg agaagattag catgttgacg ggctatgatt
     6001 acagtatggc caggatcata gatgatgctg gggttgacat cattctggtc ggtgattctg
     6061 cctccaatgt catggccggt catgaaacaa cactccccat cacgctggac cagatgatct
     6121 accatgcttc atcagtcatc agggcagtta aacgcagctt tgtggttgtt gaccttcctt
     6181 ttggttctta ccagggagat agcaagaaag cactcaacag cgccattaga atcatgaagg
     6241 aatccggagc ccatggggta aagcttgaag gagggcgtga agtgggagag tcggttaagc
     6301 gtatcctcac ggcaggaatt cctgtcatgg gccaccttgg attgacccca caaagtatct
     6361 acaaattcgg tacctattcc gtacgtgcca aagaagaaga agaggctcaa cgtctggttg
     6421 aggattccca ataccttgag tccctggggt gtttttctat cgtgttggag aagattcccg
     6481 cttcattggc tgagaaagtc gccacgaccc tgaccattcc tgttatcggt attggcgcag
     6541 gtggaggtgt agatggacaa gtgctggtga cacatgacct tttggggatc aataaagaat
     6601 tcaagccgcg atttctcaga ctgtatgaag acctttattc ccgaatgttc gatgccttct
     6661 ctgcttatat cagagatgta aaatccggtg aatttccgaa taagagtgaa caatactaat
     6721 tgaactgcct ttaattatcc ttttaacaga ataactactg ctcaatgaaa ttaaaatccc
     6781 tcaagcgccc cctgattgtc ataggagcta tgataattgt agctgcattt atttttaaag
     6841 gcagcattgt cccaaatgtc acttctgcag cggaaacgaa atttattttt gttcgaaaag
     6901 gtcaaagcct gaagagcctt gcagactcct tggaagcagg aggcttggtc aaaaacggct
     6961 ctgctatttt gaaaactgcc ggtatcatgg gctttaagga ttcggacctg gttccgggta
     7021 gatataaggc agaaaaaggc tggaataata tccgcctgtt agacttcctc tctcgcggga
     7081 aacagacgcc acttaatctt gttatcaacc caatgcgcaa ggtggaaaat gtcgctgcgt
     7141 ttctggggag taagatggaa catgattcgc ttgactttat tatagcacta tctgattcga
     7201 cctttctcga ctcttttgaa gtcaacaccg ctaatgccat gaccttgttt attccgaata
     7261 cctatcaggt ttactggaat gctaaaccta aggacttttt acgtagaatg aagaaggaga
     7321 cggatagatt ttgggcaaaa gccggccgta tggataagtt gaaagcgctg ggtatgacaa
     7381 aggaacaagt ctatac
//
LOCUS       DAOQQN010000131         7388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4037_length_7388_cov_5.895541, whole genome shotgun sequence.
ACCESSION   DAOQQN010000131 DAOQQN010000000
VERSION     DAOQQN010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4037_length_7388_cov_5.895541"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /locus_tag="PLG24_06295"
     CDS             <1..1520
                     /locus_tag="PLG24_06295"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HMZ24533.1"
                     /translation="SSSIPCVTHNGKNSGMTDSLAAGLFRPSRLLLLINLFLACCIIL
                     AIYFDQYWLIALPAVFLLGFVLLRDLRFLYIIMMMSVPISIEYSIGSFTLDAPDELLN
                     IFFLALMPGFLLFNYKNINFSFVRHPIVLFLAASFLLSIITTIFSVNVFLSVKYLLAK
                     AWYIVAYFGMTALFIKSWKDVKTLAIAVGFTAVLTMVYVMSRHAVRGFSFDSINSCVG
                     PFYSNHVNYAIQLLVIFPLIWLLGRIHQLKWKQNYLYIALMLLFIAGIYFSYTRAAIG
                     ALLLLIPFYYIVKWKLMKPAIILSIIGVFSYSIYLIKDNKFFDYAPDYNKTISHTDFN
                     TLLTATYNFEDISTMERVYRWVAARYMIADRPMIGVGPNNFYDTYSAYTLKSFRTYVS
                     DNPDHSTVHSYFMLLAIEQGILAPILFVWMVAWAMIAGQRMYHRETDKRNQYVIMAAM
                     MILFAMLVVLTINDVIESDKEGSFFYVALALIMRFHWKSIKGTDNPTSPETGKAQ"
     gene            2061..2876
                     /locus_tag="PLG24_06300"
     CDS             2061..2876
                     /locus_tag="PLG24_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HMZ24534.1"
                     /translation="MKRIITILLAILGVTFAADAQKIKGSETVLPISEKAAQVFMKSN
                     NSGNVTVTGGGSGVGISSLIGRSADIAQTSRKMKFSEKQKFQRSGKAVKEVIIAYDGL
                     AVIINPANKIKKLTREQLEDIFTGKIKNWKDVGGADMKVIPYARETSSGTYEFFKEVV
                     LKNKNYLSGIMSMPANGSLIQSVSQTKGAIAYVGLAYVSASVKTISVSFDKGKSYVFP
                     SETSVKNKTYPVVRPLYYYYLTKSEFLVKKFIDFILSPAGQKIVREVGYISVN"
     gene            2900..4114
                     /gene="pstC"
                     /locus_tag="PLG24_06305"
     CDS             2900..4114
                     /gene="pstC"
                     /locus_tag="PLG24_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HMZ24535.1"
                     /translation="MAEKKFYKRLLEKTIEQLLRISGTVTSITILFITLFLFKEGINL
                     FNTPDIEDGYGLYVHVANQKEQLSPEEIKEIFDSEITNWNQLGWKDAEIKIFRIDDVF
                     EIYPESEMGENYALLPGKLAEIISKEENIIAFLPKQFAPNAPEVKELKTGNIRWQDYF
                     LGKEWLPTASPSPLFGALPLIAGTLFVSIIALLIALPLGLGVAIYLSELAGEGQRRIL
                     KPVIELLSGIPSVVYGFFGLVVLAPLVQKIFNLPVGETALTGSLILAIMALPTIISVS
                     EDALRNTPREMREASLALGASRWQTIYKVVIPYAKSGISAAVVLGIGRAIGETMAVLM
                     VTGNAAIIPQSLFDPVRTIPATIAAELGEAPTGSAHYQALFLLGCILFILTMAISVIA
                     ELISKKKSYKVH"
     gene            4117..4959
                     /gene="pstA"
                     /locus_tag="PLG24_06310"
     CDS             4117..4959
                     /gene="pstA"
                     /locus_tag="PLG24_06310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HMZ24536.1"
                     /translation="MNKKISEFVAFSIFRLFGIIVVGILCSILGFIIYNGIKVINWEF
                     LTTFPKEGMTSGGIFPAIVGTCYLIIGSMLFAFPLGVMSAIYTNEYGGQSWIVRLIRM
                     MTNNLASIPSIVFGLFGMALFVNKLKFGDSILAGSLTLGLLALPLIIRTTEEALKAVP
                     DAYRSGSLALGATKLQTIFRVVLPAALPNIFTGLILSIGRVSGETAPILFTVAAYFLP
                     KLPSGIFDQAMTLPYHLYVLATSGTDLEASRPMAYGTAFVLIVIVLIMNLIASFLRRR
                     LERK"
     gene            4986..5732
                     /gene="pstB"
                     /locus_tag="PLG24_06315"
     CDS             4986..5732
                     /gene="pstB"
                     /locus_tag="PLG24_06315"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HMZ24537.1"
                     /translation="MIEAKNINFYYGSFHALKNISMQIKANTVTAFIGPSGCGKSTFL
                     RLFNRMNDLIEHTRLEGICSVNGRNIYDKSVNVDELRKKVGMVFQKPNPFPKSIFENV
                     AYGLRVNHIGDKKYIAQRVEESLKSAALWDEVKDKLHQSAFQLSGGQQQRLCIARTLA
                     IEPSVILMDEPASALDPISTSKIEELIHELKSKYTIVIVTHNMQQAARVSDTTGFFML
                     GELIEYSDTKKMFLNPEKEQTQNYITGKFG"
     gene            5953..6624
                     /gene="phoU"
                     /locus_tag="PLG24_06320"
     CDS             5953..6624
                     /gene="phoU"
                     /locus_tag="PLG24_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HMZ24538.1"
                     /translation="MKHNEKEIQLLKDEINQMWRLVISQMEKAKQAFLNNDAELALEI
                     ISLEKRVNAFELKIESNCENYIALYSPVAIDLRLVLSIMKISIALERIADYADGIAHH
                     VIDRDCDSLDHKVIEELELERMFDILLNMMTDSYAAINAENTASSGKILARDAEVDDI
                     YELAFSRLGEILKNHPDQIPCGLKLILLIRKMERIGDHCSNIVEDIVFYIEARVLKHS
                     GDRAE"
     gene            6658..7347
                     /locus_tag="PLG24_06325"
     CDS             6658..7347
                     /locus_tag="PLG24_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ24539.1"
                     /translation="MPQRILLVDDDRNISEILEFNLRNEGFEVNAVYSAEEALKLQIT
                     EYHIILLDVMMGGMSGYKLAEKLRNDNYSVPIIFLTARDTENDMLTGFSVGGDDYIPK
                     PFSIKEVIARVKAVLRRSRVESGTDPKSDKLKIGPITIDLEVKEVMIDHERISLTKTE
                     FELLVLLASKPDSVFSRERILRNIWNETPYISERTVDVHLARLRKKLGIYGAAISNRP
                     GYGYRFNTEAL"
BASE COUNT         2215 a         1514 c         1577 g         2082 t
ORIGIN      
        1 catcatcgtc gattccctgc gtgacccaca atggaaaaaa ctctggaatg actgattcct
       61 tagccgccgg ccttttcagg ccatcgcgcc tgttattgct gatcaacctg tttttggcat
      121 gctgtatcat tcttgctatt tactttgatc aatattggct cattgcccta ccggcagttt
      181 ttctgctcgg attcgttctg ctaagagacc tgaggttcct gtatatcatc atgatgatga
      241 gcgtaccaat atccattgaa tattccattg gtagctttac actggatgca ccggatgaat
      301 tgctcaatat ttttttcctg gccttgatgc ctggattctt gttgttcaat tacaaaaata
      361 tcaatttcag cttcgttcgc catccgatag ttctttttct ggcagcatct tttcttttat
      421 ccatcataac gacaatcttc tctgtcaatg tttttctttc agtgaagtat ctcctcgcaa
      481 aagcctggta tattgtagcc tattttggca tgaccgcact tttcataaag tcctggaaag
      541 atgtcaaaac gctggctatt gccgtcggat ttactgctgt gctgaccatg gtttatgtca
      601 tgtcaagaca tgcagtacga ggcttttcct ttgattccat caactcatgt gtcggacctt
      661 tttattccaa tcatgtcaac tatgcaatcc aattgctcgt tattttcccg ttgatatggt
      721 tgcttggcag aatacaccaa ttgaaatgga agcaaaatta tctatacatc gccttgatgt
      781 tactgtttat tgcggggata tactttagtt acacacgggc cgccatcggt gctttattgc
      841 tgcttattcc cttttattat attgtcaagt ggaagctgat gaagccggct atcatccttt
      901 ccatcatcgg agttttttca tactcaatct atctgattaa ggacaataaa tttttcgatt
      961 atgcgcccga ctacaacaaa acaatctctc atacggattt caacacatta cttactgcga
     1021 catacaactt tgaagatatc tccaccatgg agcgggtgta tcgttgggtc gccgcaagat
     1081 atatgatagc cgacagacct atgataggag ttggtcccaa caatttttat gacacttatt
     1141 ctgcttacac cctcaaatcc ttcaggactt atgtcagtga caatcccgat cacagcacgg
     1201 tacactctta ttttatgttg ctggcaatag aacagggaat cctggctccc atcctatttg
     1261 tctggatggt tgcctgggca atgattgcag gacaacgtat gtaccatcga gagacagaca
     1321 aaaggaacca atatgtcatc atggctgcta tgatgattct ttttgctatg ctggtcgttt
     1381 taacgatcaa tgatgtcatc gagtccgata aagaaggttc ctttttttat gttgcactgg
     1441 cattgattat gagattccat tggaaaagca taaaagggac agacaacccg acatcccctg
     1501 aaacaggaaa agcccaatag attcaaagcc ctttaataga ttgatataat tttgattatt
     1561 agctgtattt gtatataata attttatata taaattatta tattggttga ttttcactaa
     1621 aatattttgg atattcctgg ataaacaata gtatttgaaa ctggtcaatt gtcactcgat
     1681 tggttaatca cacacaggtg attccggctg ataatctgca atgattaatg caacctaata
     1741 ccgaaggtac attcgttata gaccaatttc attgatttat acgactgtaa cctcggcatc
     1801 cccgaatcgc ttagctctcg ggacaagtat accaaaaatt tatggaatat tatataaatc
     1861 atcttggtat aaggtattaa agccgataat ataaaacata ataattacta ataaaatccc
     1921 ctcctgcctc ttaagtgaaa aaatgagaga taaagtattt atgctttgtc tgcctaaaaa
     1981 ttctgaagtc ttaaccaaat tgtaatatta tctcaaatgt attgaaacat cggtgcctca
     2041 attttgcgca aattaaataa atgaaaagga ttatcacaat tttattggcc attttgggtg
     2101 tgacttttgc tgccgatgct cagaaaataa aaggttccga gaccgtcctc ccgatttctg
     2161 aaaaagcagc tcaggttttc atgaaatcaa ataattcagg taatgtgact gttacaggtg
     2221 ggggcagcgg tgtgggaatt tcatccttga ttgggagatc ggcggatatt gctcagacat
     2281 cccgaaaaat gaaattttca gaaaaacaaa aattccagag aagtggcaag gctgtcaaag
     2341 aagttataat cgcctatgat ggacttgctg taatcatcaa tccggccaac aagatcaaaa
     2401 aactgacaag agaacaactg gaagatatat ttacaggaaa gatcaaaaac tggaaggatg
     2461 tcggtggtgc tgatatgaag gtcattccct atgccagaga gacttcgtcc ggcacctatg
     2521 aatttttcaa ggaagttgtg ctcaaaaaca aaaactacct gtccggaatt atgagcatgc
     2581 cggccaatgg ctccctcata caatccgtaa gccaaaccaa aggagctata gcctacgtcg
     2641 gcctggctta tgtcagtgcg tctgtaaaga caatctctgt gtcctttgac aagggcaagt
     2701 cgtatgtctt tccctccgaa acaagtgtaa aaaacaagac ctatcctgtt gtcagaccat
     2761 tgtattacta ttatctgact aaatcggaat tcctggtgaa aaaatttatc gatttcatac
     2821 tttccccggc tggtcagaag atagtcaggg aagtcggtta catcagtgta aattgatagt
     2881 ttcgaactaa tgctaagtga tggctgaaaa gaaattttat aagcgccttt tagaaaaaac
     2941 aattgaacag ttattaagga tcagtggtac cgttacaagt atcacaatcc tgtttattac
     3001 cttatttctt tttaaagagg gaataaattt gttcaacact cccgatatag aagatggata
     3061 cgggctttat gtgcatgtag cgaaccagaa ggaacaactg agtccagaag agataaagga
     3121 aattttcgat tccgaaatca ccaactggaa ccaattggga tggaaagatg cagagattaa
     3181 aatattcagg attgatgatg tatttgagat ttatcctgaa agtgaaatgg gggagaatta
     3241 cgctttatta cccggcaagc tggcagagat catcagcaaa gaagaaaata tcatcgcttt
     3301 cctgcccaaa caatttgcac caaatgcgcc tgaagtgaag gagctcaaaa ctggtaacat
     3361 tcgatggcag gattatttcc ttggaaaaga atggttgcca acagcctctc cttctccact
     3421 ttttggtgca ttgccactga tagccggaac attgtttgta agcattatag cattgctgat
     3481 agcattgccg ctgggtctgg gagtcgccat ctatctttcg gaactcgccg gtgaaggaca
     3541 gcgtcggata ttgaagcctg taattgaact attatcgggg atcccatcgg tggtgtatgg
     3601 gttcttcgga ttggttgtgc tggcacccct cgtgcagaag atattcaatc ttccagtagg
     3661 agaaaccgca ctgacaggaa gccttatcct ggcgatcatg gcgttgccaa ccattatttc
     3721 agtttctgaa gatgctttgc gaaacacgcc ccgggaaatg cgtgaggcaa gcctggctct
     3781 gggtgccagc cgatggcaga ccatctacaa agtggtgata ccttatgcca aatcaggtat
     3841 ttctgctgct gtcgtattgg gaatcggaag ggctatcggc gaaacaatgg cagtattaat
     3901 ggttacagga aatgctgcca tcatccctca atccctcttt gaccctgtgc ggacaatacc
     3961 tgctaccatt gcagcagaac tcggagaagc tccaacagga agcgcacatt atcaggcatt
     4021 gttcctgctt ggctgtattc tatttattct cacaatggca atatcggtta tagctgaatt
     4081 aatttcaaaa aagaaaagtt ataaagtaca ttagttatga ataaaaaaat aagtgaattt
     4141 gtagcttttt ccatattcag actattcggt atcattgtgg taggcattct gtgcagtatt
     4201 ctgggattta ttatctataa tggaattaag gtaatcaact gggaatttct cacaaccttc
     4261 cccaaagaag gtatgacctc aggcggtatc ttcccggcta ttgtaggtac ctgttatctg
     4321 atcatcggca gcatgctgtt tgcctttccc ctgggagtca tgtctgccat ttataccaac
     4381 gaatatggcg ggcaaagctg gatagtcaga ctcatcagga tgatgaccaa caatctggca
     4441 agcataccct ctattgtatt cgggctattc ggaatggcct tatttgtgaa taagcttaag
     4501 tttggcgact ctatattggc gggttcttta accctgggtt tgctggcctt gcccttaatc
     4561 atccgcacca cggaagaggc attgaaggcc gtacctgatg cgtatcgtag cgggagcctg
     4621 gcccttggag ccaccaaatt acagaccatc tttcgggtag tattacctgc cgcgttaccc
     4681 aacatattta ccggattgat tctttcgatc ggacgggtat cgggtgagac agcccccatt
     4741 ctatttaccg tggcagcata ctttctgcca aaacttcctt ccggtatctt tgatcaagcc
     4801 atgacgcttc cttaccacct gtatgtgctg gccacaagcg gtacagatct cgaagcatcc
     4861 aggccaatgg cctacggtac ggcttttgtc cttatcgtca tcgtactgat aatgaacctg
     4921 atcgcctcat ttttaaggag gaggctggaa aggaaataaa tgttttataa aaatcaaaca
     4981 tgaaaatgat agaagcaaaa aatatcaact tttactatgg cagctttcat gcgctgaaaa
     5041 atatcagtat gcagattaag gccaatacgg ttactgcatt catcggtcca tcaggatgtg
     5101 gcaaatcaac cttccttcgc ctgtttaacc ggatgaacga cctgatcgag cacaccagac
     5161 tggaaggtat atgtagtgtc aacggtcgta atatctacga taagagtgtc aatgtcgatg
     5221 agctgagaaa aaaagttggt atggtatttc aaaaacccaa tcccttcccg aagtccatat
     5281 tcgaaaatgt agcttacggg cttcgtgtca atcatatcgg tgataaaaaa tatattgctc
     5341 aaagagtcga ggaatcctta aaatcagccg ccctctggga tgaagtcaag gataaattac
     5401 accagtcggc atttcaactc tcagggggcc agcaacaacg gctctgcatt gccaggacac
     5461 tggccattga gccgtcagtc atcctgatgg acgaaccggc atcagcacta gaccccattt
     5521 ccacctccaa gatcgaagaa ctaatccatg aattgaagtc aaagtacacc atcgtcatcg
     5581 taacccacaa catgcagcag gcagcaagag tgagcgatac aacgggattc ttcatgctgg
     5641 gtgaattgat tgaatacagt gatactaaga aaatgtttct caatccggag aaggaacaaa
     5701 cacagaatta cattacgggg aagtttggtt aaaagatttt ttgccaacag agaatgctga
     5761 atgatgcaga aatccatcac agatttagta gtaaaaggat taataggcat caaaaaggat
     5821 agcaaaaagt ttaatgaaag aaaattgata ataatcataa ataatctcat tgccagataa
     5881 aacatttacc tcggaagatg gcgtcacgaa gaatgataaa aaaccgaagg ttgtaacaat
     5941 aatcaaagta aaatgaaaca caacgaaaag gaaattcaac tattaaagga tgaaatcaac
     6001 caaatgtggc gattggtcat atctcaaatg gaaaaagcga aacaggcttt tttgaataat
     6061 gatgcagaac tcgccctgga aattataagt ctggaaaaga gggtgaatgc tttcgagttg
     6121 aaaatagaaa gtaattgcga aaactatatc gctctgtaca gtcctgtagc aatcgatctg
     6181 agacttgtat tgtccatcat gaaaatcagc attgccctag aaaggattgc cgactatgca
     6241 gacggaattg cccaccatgt aattgaccgg gattgtgact cactggatca taaagtaatc
     6301 gaggagctgg aactggaaag aatgtttgat atcctgctca atatgatgac tgatagctat
     6361 gcggccatca atgctgaaaa tacagcatct tccggcaaga tattggcacg tgatgctgaa
     6421 gtggacgaca tctacgaact ggctttcagc cggcttggtg aaatactgaa gaatcaccca
     6481 gaccagattc cgtgtggatt gaaactgatc ctgttgattc gcaaaatgga aagaataggt
     6541 gatcattgca gcaacattgt tgaggacata gttttctata tcgaggcaag ggtgttgaaa
     6601 cacagtggag acagagcgga ataatttgct aattttgctg caaatagctt gaatacaatg
     6661 cctcagagga tattgctggt ggatgatgac cggaatattt cagaaatcct ggaattcaac
     6721 cttcgaaatg aaggattcga ggtaaatgct gtatattctg cggaagaagc cctgaagctt
     6781 cagattactg aatatcacat tattctgctt gatgtaatga tgggcggaat gtccggctac
     6841 aagctggcgg agaaattgcg aaacgataac tattcggtcc caatcatctt ccttacggcc
     6901 agggacaccg aaaatgatat gctgacgggt ttttcagtcg gcggggacga ttatatccct
     6961 aagccattct cgatcaagga agtcattgca agggtaaaag cagtattgag gcgtagccgg
     7021 gtagaatccg ggacggaccc gaagtccgac aaactcaaaa tagggccaat aaccatcgac
     7081 cttgaagtca aggaagtgat gatcgaccat gaaagaatct cccttacaaa aaccgaattt
     7141 gagttgctcg ttttgctcgc atccaagcct gacagcgttt tttcaagaga gcgcatcctc
     7201 cgcaatatat ggaacgagac accgtatatc tccgaacgta ctgtggatgt acacctggca
     7261 cggttgagaa aaaaactggg gatctatgga gcagcaataa gcaatcgacc gggctatggg
     7321 tacagattta acacggaagc tttatgaaac tcacttttcg ccatcggcta ttcctgtatt
     7381 ttgccatt
//
LOCUS       DAOQQN010000132         7318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4106_length_7318_cov_5.572491, whole genome shotgun sequence.
ACCESSION   DAOQQN010000132 DAOQQN010000000
VERSION     DAOQQN010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4106_length_7318_cov_5.572491"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..104)
                     /locus_tag="PLG24_06330"
     CDS             complement(<1..104)
                     /locus_tag="PLG24_06330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ24540.1"
                     /translation="MKRVIISGGGTGGHIYPAIAIADAVKAALDNVEL"
     gene            complement(101..1288)
                     /locus_tag="PLG24_06335"
     CDS             complement(101..1288)
                     /locus_tag="PLG24_06335"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HMZ24541.1"
                     /translation="MKTKEWLSANFKGDPILWILLTLLALGSLLAVYSTTGGYSQIGK
                     SHEATNEYFLFKQLVFLVVGVGLAYIGHRVNFKRYSAIALPFLVFSFLLLVLVLFFGN
                     EINGAKRWLTVPLVGLSIQPADIAKMALMIYLARVISGSQTMIKQDISIFWMLIIPTL
                     MVCLFILPNNLSTAGLLFVTSIIVMFIGRIPIKYILYIFGIGMTMFLFVFLIGYFSGT
                     GTRAVTWANRITAFVGGEESFQSQQARIAIANGNVIGVGPGNSMQRNFLPYSHADFIY
                     SIICEEFGIVGAIGVMLIYLLMLYRVIRIVKRSPKTFGAMLAIGLCINIVIQAYANIM
                     VNVGLIPDTGVTLPIISLGGTSLIFTCLSFGIILSVSRYIETREEVMSAVVLNTETKT
                     TEG"
     gene            complement(1356..2663)
                     /gene="murD"
                     /locus_tag="PLG24_06340"
     CDS             complement(1356..2663)
                     /gene="murD"
                     /locus_tag="PLG24_06340"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HMZ24542.1"
                     /translation="MKVVVIGAKESGVGAALLCKQMGYDVLVSDNGPVIEPFRMELER
                     SKIAFEENGHSSGMSDRFDLMVISPGVPSTVPVIRDFTNSGKEVISEIEFAWRHIGDG
                     KVIGITGSNGKSTLSSLIYHVMEELDRDVTLVGNIGYSFAKMIATTRSQYYVCELSSF
                     QLESIVDFRPHVAIITNITPDHLDRYDHSMEKYAAAKFRITENQTSSDVLIINADDPF
                     SMDYFKQVETKAQIKYISSDRISVDSYISGSGKVYDLSNSQLRGRHNRANMAQAIEAL
                     EFIGEKQYQIEQNISSFNGLAHRLEYVATVGGVDFINDSKATNVDSVMYALDAMTQPI
                     VWIAGGTDKGNEYNAIMPLVKDKVKHLICLGIDNTKLIDSFHNIVGSIEEAGSAEEAV
                     RKAMKAATPGDVVLLSPACASFDLFKNFEDRGEKFKKAIRKLT"
     gene            complement(2669..3928)
                     /gene="mraY"
                     /locus_tag="PLG24_06345"
     CDS             complement(2669..3928)
                     /gene="mraY"
                     /locus_tag="PLG24_06345"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HMZ24543.1"
                     /translation="MLYYLFEYLYKDLNIPGTGVFRFITFRAGLAIVLSLLISIVFGK
                     RIIQSLRRRQIGETVRDLGLEGQKEKEGTPTMGGIIIVLAIVVPCLLVARLDNVYIIL
                     MLISTLWMASIGYLDDYIKVVKKDKDGLKGVFKIIGQVGLGLLVAITMLMSNDVTVRM
                     TPQEAEQGEYKIVKTVMVEDPHNAGKLVEMVHAKAAITNVPFFKNNELDYGRLLDFMH
                     MNKEVMLWIVFIVAVVFIITAVSNAANLTDGIDGLATGVSATIGAVLAAFAYLSGNSV
                     FADYLNITFIPNSSELVIFSSCFIGACTGFLWHNSFPARVFMGDTGSLTLGGIIAALA
                     IVLRKELLLPILCGIFVVENLSVIIQRYYFKYTKKKYGEGRRVFLMAPIHHHFQKKGY
                     HEAKIVTRFWIVSIILAVMTILTLKIR"
     gene            complement(4158..5615)
                     /locus_tag="PLG24_06350"
     CDS             complement(4158..5615)
                     /locus_tag="PLG24_06350"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HMZ24544.1"
                     /translation="MPVIQLNVLLKSAGITHSSSGNLVEISNIAIDSRKVSKGSLFVA
                     IKGSEQDGHKYIRKAIDAGASAIVCQEKADVPDDILMVLVEDSADAAGRLASAFYGHP
                     SEALKLVGVTGTNGKTTTVTLLYQLMKQLGHRTGLISTVENIVNGRVVVATHTTPDPV
                     ELNALLAEMVAVGCEYAFMEVSSHAAHQKRISGLRFEGAVFTNITHDHLDYHKTFDDY
                     IAAKKSFFDGLASGSFALVNGDDRRSDIMLQNTKASKHFYSLRRSSEFKGKIFENTLE
                     GLEMEIDGLHAHLLLIGEFNAYNALCAYGICRLLGFEREVILTGLSALPGARGRFETV
                     RDMNRGVLYIVDYAHTPDALEKVLKTIEVMKQKGGIITVVGCGGHRDKEKRPKMGAIA
                     ASLSREAIFTDDNPRDENPADITAAMMDGVDDADRNKVLIINDRAQAIKTAAKLASRG
                     DIVLVAGKGHETYQEGMNGKIDFDDRRVIEDIIGR"
     gene            complement(5617..>7318)
                     /locus_tag="PLG24_06355"
     CDS             complement(5617..>7318)
                     /locus_tag="PLG24_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HMZ24545.1"
                     /translation="HEKMKLVKGFPLFRMGKNKSGVIFEMKQSRVRPYGDLALRTIGY
                     ARKNSQSIGLEASYDEYLKGEAGKRMVQYISPTKYIPINDLTEIEPKNGDDIHTTINV
                     QMQDIAGQALRTALDYHKASYGTVIIMEVKSGNIKAMSNLKRDGGVFTEDYNYAAGLS
                     TEPGSTFKLASVMAILEDKMMSLDKEFEINYGKSIISGREMKDSEGHGHAKLSLRRAF
                     ELSSNIGISKAVVEAYGGSREGSFKFVDRIKQFHLDKITGIDLPGEGKPLIKTPYNLK
                     QLWSKSTLAWMAHGYETQLTPLQLLTFYNAVANNGKMMQPRLVTGVWSHGEMVKDFQP
                     KVLVDKIASDTTIMLARTLLEGVVLNGTARRYKNDMYSFAGKTGTASLDYGGIKRGRY
                     RASFIGDFPADNPMYSICVSITEPREHGYHGAEVALPVFRSVADKILSFDPKFFKVTS
                     PEKNAMVVTDQLPQGEKGYRTDFLSIFNRLGVPVYSGGGITDFIVTRKVGSDVNLMAM
                     NEPPGLVPDVTGMGLRDALYALESRGLKAEVNGYGRVSTQNISPGTKVSGQKVVIRLE
                     "
BASE COUNT         2045 a         1832 c         1347 g         2094 t
ORIGIN      
        1 aacaattcta cattgtccag ggcagccttc accgcatccg caatggcgat tgccgggtaa
       61 atatggcctc ctgtgcctcc tccgctgata atcaccctct tcatccttct gttgtttttg
      121 tttcagtatt gagaacaacc gcactcatta cctcttccct ggtttcaata tatctgctta
      181 cactcagtat gataccaaaa gagaggcagg tgaaaatcaa gcttgttcca ccgagagaga
      241 tgatcggaag agtaactcct gtatccggga tcagacctac attaaccata atatttgcat
      301 aagcctgaat gacaatattg atgcacaatc caatggcaag catggcacca aaggtcttgg
      361 gcgatcgctt aactattcgg atcactctgt aaagcatcaa gagataaatc aacatcacgc
      421 ctatagctcc aactatacca aactcctcgc atattatgga atagataaaa tcggcatgtg
      481 agtaaggaag aaagttcctt tgcatactgt tacccggtcc gacgcctatg acatttccat
      541 tggctatggc aatacgggcc tgctgtgact ggaaggattc ttcaccgccg acaaaggcgg
      601 ttattctatt ggcccaggtt actgctctgg ttcctgtccc ggaaaagtaa cctatcagga
      661 aaacgaatag aaacatagtc atgcctattc cgaaaatgta caggatatac ttgattggaa
      721 tccttccaat aaacattaca atgatagatg tgacgaacag aaggcctgca gtactcaagt
      781 tgttgggtaa aatgaaaagg cataccataa gtgtcggaat gatcaacatc cagaaaatgc
      841 tgatatcctg ttttatcata gtctgagaac cgctgattac ccgggccaga taaatcatca
      901 aagccatctt agcaatatca gcgggttgaa tggaaagtcc aactaaagga acggtcagcc
      961 atcgctttgc tccattgatc tcattaccaa aaaatagaac caaaactaaa agcaaaaaag
     1021 aaaagaccaa aaatggtaaa gcgatggctg agtaacgctt gaaattgacc ctgtggccaa
     1081 tataagcaag tcccactcct acaaccagaa aaactaactg cttgaacaag aagtattcat
     1141 tcgtggcctc atgactttta ccaatctgtg aataacctcc tgtggtacta taaactgcaa
     1201 gtaagctgcc aagggcgagc aaggtgagca ggatccatag gatcggatct cctttaaaat
     1261 tcgcacttaa ccattctttt gtcttcattc tgtagttgta gttttcttaa agaccaattt
     1321 tttaatacca gaccttagtt ttcagcccaa ataatttatg ttagttttct gatagccttt
     1381 ttaaatttct cgcccctatc ctcaaagttt ttgaacaggt cgaaacttgc acatgccggt
     1441 gaaagcaata ccacatcacc cggagttgca gccttcatag ctttacgcac tgcttcttct
     1501 gccgagcctg cctcctctat actgcccacg atattatgga aactgtcgat gagtttggta
     1561 ttgtcaatac caaggcaaat aagatgcttg accttatcct ttactaaagg catgatagca
     1621 ttgtactcat tgcctttatc tgtccccccg gcaatccaca cgataggctg agtcatagca
     1681 tccagcgcgt acatgactga atcgacatta gtagccttac tgtcattgat aaaatcaaca
     1741 ccacccaccg tagcaacata ctcaagccga tgtgccagac cattgaagga cgagatattc
     1801 tgctcgattt gatattgctt ctcacctata aactccagtg cttcaatagc ctgtgccata
     1861 ttggcacgat tgtgccttcc tctcaactga ctgttactca ggtcatacac ctttccagac
     1921 ccggaaatat aactgtcaac agaaatccga tctgaggaga tatatttaat ctgggccttt
     1981 gtctcaactt gcttaaaata atccattgaa aagggatcat cggcattaat gatgagtaca
     2041 tctgatgagg tttgattctc cgtaatcctg aacttagccg ccgcgtattt ctccatgcta
     2101 tgatcatatc tgtccaaatg atccggcgtg atattggtaa tgattgccac atgcggtcta
     2161 aaatcaacaa tactctccaa ctggaagcta ctcaactcgc atacatagta ttgtgaacgc
     2221 gtagtggcta tcattttggc aaagctgtaa ccaatattac caaccagggt aacatcccga
     2281 tccaactctt ccatcacatg atatatcaaa gaagagaggg tgcttttgcc attagatcct
     2341 gtaataccta ttaccttccc gtcaccaata tgtctccagg caaattcaat ctcagaaatt
     2401 acttcctttc cactatttgt gaaatcacgg ataactggta ctgttgaagg aacaccagga
     2461 ctgataacca tcaggtcaaa ccgatcgctc attccggatg aatgtccatt ttcctcaaaa
     2521 gcaatctttg atctctctaa ctccattcga aaaggctcta taactggtcc attgtcactg
     2581 accaggacat catatcccat ctgcttgcac agcaatgctg ctccaactcc actttcttta
     2641 gctcctatga ctacgacctt cataccgatt atctgatttt gagggttaaa atggtcatga
     2701 cagctagtat gatggacacg atccaaaatc ttgtaacaat tttagcctcg tggtatcctt
     2761 tcttctgaaa atgatgatgg ataggtgcca ttaaaaagac acgcctgcct tcaccatatt
     2821 ttttcttggt atacttgaag taatatcttt ggatgatcac agagagattc tcgacaacaa
     2881 aaattccgca aagtatcggc aacaagagtt cttttcgcaa tacaatagct aaagctgcta
     2941 tgataccgcc aagtgtcagg ctgcctgtat cacccataaa tacccgggca ggaaatgaat
     3001 tatgccataa gaatccggta catgcaccga taaagcatga actgaatatt actaattcac
     3061 tactattggg aataaaagtg atgttgaggt aatccgcgaa aacagagttt ccgctcaaat
     3121 aggcaaaagc agccagtacg gcaccaatcg tagcggacac accggtagcc agtccgtcaa
     3181 ttccgtctgt cagattagcc gcattgctga ccgcagtgat tatgaatacc actgcaacta
     3241 tgaatacaat ccaaagcatg acttccttgt tcatatgcat gaaatcaagt agccttccat
     3301 agtccaattc gttgttctta aaaaagggaa catttgtaat ggctgccttg gcatgtacca
     3361 tttctacaag cttaccggca ttatggggat cctcaaccat gacggttttt acgatcttat
     3421 attctccctg ttctgcttct tgcggggtca tccttacggt aacatcatta ctcatcagca
     3481 tggttattgc aaccaataat cccaagccga cctgaccaat tatcttgaaa acgcctttca
     3541 aaccgtcttt gtccttcttc actaccttaa tgtaatcatc caggtaaccg atgcttgcca
     3601 tccacaatgt ggaaattaac atcaggataa tatagacatt gtcaaggcga gccaccagca
     3661 gacatggcac tacaattgcc aggacaatga tgataccgcc catggttggt gttccttcct
     3721 tttctttttg gccctcaaga ccaagatctc gaactgtctc gccaatctga cgacgccgga
     3781 gtgattgaat gattcttttt ccaaaaacta tagaaatcaa taaagacaat acaatagcaa
     3841 gacccgctcg aaacgtgata aatctgaaca ctccggtgcc cgggatattc agatctttgt
     3901 ataagtattc aaacaaataa tacagcatct cgcctatgta ataatttttt aatcggtgta
     3961 gcttttcaga tacactttgt cttggtttct ttggttttcc tcaatcttca taacctgatg
     4021 ttccaaataa aaatggtgag caggggcaac cagtctgcgc tggcattaga accgttatga
     4081 cagccagttt ccggattgcc tgacctgctc attccaacca aatttaaaaa tagaacctac
     4141 gtctttccga cagaacttta cctgccgatt atatcttcaa tgacccttcg gtcgtcaaaa
     4201 tcaattttcc cattcatccc ctcctgatat gtttcatggc ctttacctgc cacaagcact
     4261 atatcccccc tggatgccag tttagccgct gttttgatgg cctgggccct gtcgttgatg
     4321 atcagaactt tatttctgtc ggcatcatcc actccgtcca tcattgctgc agtaatgtcc
     4381 gccggatttt catcccttgg gttatcatcg gtaaagatgg cttctcgact taagctggca
     4441 gcaattgctc ccattttagg ccgcttctct ttgtcacgat gacctccaca acccactact
     4501 gtaatgattc cgcccttttg tttcatcacc tcgatggtct taagtacttt ctccaacgca
     4561 tccggtgtat gggcataatc aacgatatac aatactcccc tattcatgtc ccggactgtc
     4621 tcaaatctac ctcttgctcc cgggagagca cttaatcctg taagaatcac ctctctttca
     4681 aacccgagta acctgcatat gccgtaagcg cataaagcat tataagcatt gaattcccct
     4741 atcagcagca gatgagcatg caatccgtca atttccatct ccagaccttc caaggtattc
     4801 tcaaagattt tacctttaaa ttctgagctc cttctgagac tatagaaatg cttagatgcc
     4861 tttgtattct gcagcatgat atcgcttcga cggtcatccc cattgaccag ggcaaaactt
     4921 cctgaagcca ggccatcaaa aaatgatttc ttcgctgcta tataatcatc gaaggtcttg
     4981 tggtaatcaa gatgatcgtg ggtgatattg gtaaatacag caccttcaaa tctcaaacca
     5041 ctaatgcgct tctgatgagc agcatgggaa ctcacctcca tgaaggcgta ttcacacccg
     5101 acagcaacca tttcagccaa aagagcatta agctctaccg gatcaggtgt agtatgggtc
     5161 gcaaccacca ctcttccatt cactatattt tcaaccgtag aaatcagacc tgtccgatgc
     5221 cccaattgtt tcatcaactg gtaaagcaaa gtgacggtgg tagttttgcc attagttccg
     5281 gttactccca ccagttttag tgcttccgat ggatgtccgt agaaggcaga tgccaacctc
     5341 cccgcagcat ctgcactgtc ttcaaccaat accatcagga tatcatcagg cacatctgcc
     5401 ttttcctggc aaactattgc tgaagctcca gcgtcaattg ctttacggat gtatttatga
     5461 ccatcctgct cagatccttt aatcgcaacg aacaacgacc ctttcgaaac cttccggctg
     5521 tcgatggcta tattgcttat ttcgactaaa tttccggatg aagaatgggt aatgcccgcc
     5581 gacttcaaaa gaacattgag ctggatcact ggcatatcat tccaatctga ttactacttt
     5641 ctgccccgaa acctttgttc cgggtgatat attttgagtt gatactcttc cgtatccatt
     5701 cacttctgcc ttcaatcccc gactttccag ggcgtacaat gcatcacgaa gacccattcc
     5761 ggtgacatcc ggcaccagac ccggcggttc attcatcgcc atcagattca catcagagcc
     5821 aacctttctg gtcacaatga agtctgtgat tcctcctccc gaataaacag ggacccctaa
     5881 ccggttgaat atgcttaaaa agtcggtgcg atatcctttc tccccctgag gtaattgatc
     5941 agtaacaacc attgcatttt tttcaggaga agttaccttg aaaaacttcg ggtcaaatga
     6001 aagaatctta tccgccactg acctgaatac aggcaaagca acttccgcac cgtgatatcc
     6061 atgttccctt ggctctgtaa tagatacaca aatggaatac attggattat ctgccggaaa
     6121 atcccctatg aatgaagcgc gatatctccc gcgcttgata ccgccataat ccaggcttgc
     6181 tgtgccagtc ttgcctgcaa agctgtacat atcattttta tatcttcgtg cagttccatt
     6241 gaggacgact ccttccagca aggtccgtgc aagcataatt gttgtatcac ttgctatttt
     6301 gtcgaccaag accttaggtt gaaaatcttt aaccatttct ccgtgagacc agacgcctgt
     6361 cactaatcga ggctgcatca tcttaccatt attcgcaaca gcgttataga aggtcagcaa
     6421 ttgaagagga gtcagctggg tttcgtaccc gtgagccatc catgcaagcg ttgacttact
     6481 ccacagctgc ttcaagttat atggagtctt tattaaaggt tttccttcgc ccggaagatc
     6541 aatacccgtt attttgtcaa gatggaattg tttaattctg tccacaaatt tgaaggaacc
     6601 ttccctactg cccccatatg cttcaaccac cgccttgcta attccaatat tggatgaaag
     6661 ctcaaatgcc cttctgagag acagcttagc atgtccatga ccttcggaat ccttcatttc
     6721 acggccgctg atgatcgatt taccgtaatt aatctcaaac tccttatcca gggacatcat
     6781 tttgtcttcc aaaatagcca tcacggaagc gagcttaaaa gtcgaaccgg gctcggtact
     6841 caatcctgca gcgtagttgt aatcttcggt aaagactcca ccatccctct taagattgct
     6901 catggccttg atattgcctg atttgacctc catgatgatt actgtcccat aggaggcttt
     6961 gtgataatct aatgcagtcc tcagtgcttg tccggctata tcctgcatct ggacattaat
     7021 ggtagtatgg atatcatcgc cattcttggg ctcaatctcc gtcagatcat ttattggaat
     7081 atacttcgtt ggtgagatgt attgaaccat cctttttcct gcctcacctt tcaaatattc
     7141 atcataggat gcttccaacc caatcgactg agaattctta cgtgcatagc caatagttct
     7201 aagggcaagg tcaccatatg gtctaacacg actttgtttc atttcaaaaa tgactcccga
     7261 cttattcttg cccattctaa aaagtgggaa gcccttgacc aatttcatct tttcatgt
//
LOCUS       DAOQQN010000133         7300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4126_length_7300_cov_4.984541, whole genome shotgun sequence.
ACCESSION   DAOQQN010000133 DAOQQN010000000
VERSION     DAOQQN010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7300
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4126_length_7300_cov_4.984541"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /gene="pnuC"
                     /locus_tag="PLG24_06360"
     CDS             complement(<1..476)
                     /gene="pnuC"
                     /locus_tag="PLG24_06360"
                     /inference="COORDINATES: protein motif:HMM:NF016837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HMZ24546.1"
                     /translation="MEILEAVWQYFQSHTLEFVAAFFGVVSVWYSRKNDILVYPTGIV
                     SVLLYVYICLIHKLYADAGVNFYYFVMSVVGWVNWSKKKEEEFVFPISYCDRNEWIKG
                     IAVFLIILISLLFFLFYFTDSTTVVGDSLVSASAATAMWWMAKRKIESWYAWILSN"
     gene            998..1573
                     /gene="folE"
                     /locus_tag="PLG24_06365"
     CDS             998..1573
                     /gene="folE"
                     /locus_tag="PLG24_06365"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HMZ24547.1"
                     /translation="MDTNEYQDSRIDNLINHYNQIITLSGEDVEREGLLKTPKRAAKA
                     IDFLTSGYRMDAAEILRSAMFNEPYNEMIIVKDIEVYSLCEHHMLPFFGKAHIAYIPK
                     DYIVGLSKLPRVVDVFARRLQVQERLTQEILEVIDNTLKPHGVAVVIEASHMCMMMRG
                     VQKQNSVTTTSAFTGEFKNVETRNEFLQLIR"
     gene            complement(1641..3959)
                     /locus_tag="PLG24_06370"
     CDS             complement(1641..3959)
                     /locus_tag="PLG24_06370"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HMZ24548.1"
                     /translation="MFSRNLQSLTLFVFMMGIFSIAHSQTDNTIFKVEKASLSRDVLE
                     YSPLYLKGDWVYTQLDDKNVNIYSLGSDKVIPIEGFSLRRAIVTGFAKSDQGGLIAIS
                     YGDNKQNVPAKLKGNKIYFAFQDKNGIASKYFEFQLNSEAYSCTTPYLSPDGTKLYFA
                     SDMPGGYGGLDIYVCDYRGKEWSKPRNLGPKINSEKDEVYPYMIDNMLFFSSNGHGAN
                     RMDIFIADMDKDLGRLVINAGSPINSPADDYSMSFDNNTRIGYFLSDREGVNGKAFKV
                     TNEKKLILLNIKSNEDKKAISGAKVDLSRCRKNPLYANSKGSVILPVAAGEKCFIQVG
                     KVGYNSATIPIDFDEIIGLKKSININLSKEGVFYQGRVVDQDNNPADEVELSIVDQAT
                     GELQYVFTDDNGYYTIALEPLSYYMIRSKSPYYNPSESKIVTLASVPADILGTIKLVS
                     NGSRRSGIIKTSSMDDIKVTQRNESETENPSTSDFVETGVAKKIESVKGPETSVTDAQ
                     KVMDYNDNRLREETVKEEVMQENKQIAPAQSPVIVAPKTENKKEAIVKYAIQLAAISL
                     DNHNLQPYQEKTRGRGQVYIAREGNLNKVRLGFYETRQDALAIREQLPEELRKGFIVE
                     VKESEYNNRQAIPVDRKNAIDPPMNKPEKKAEPDPRIQPENKPEMKSEVKPQEKTNSE
                     PQVVQKSLEESRVEYKIRLSTLRNASLFNGSAVRQYGLIEEIKTGNLTTFYLSGFENK
                     EEVKQVIDKVKAGGFPSAQIVKLTNGEYTIDN"
     gene            complement(4014..4595)
                     /locus_tag="PLG24_06375"
     CDS             complement(4014..4595)
                     /locus_tag="PLG24_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24549.1"
                     /translation="MINFTLRLLTFCLFLTFIQCKPGDNGTSSVEATPRTDSVRFKKY
                     VPEPGDVPVDPEADDNAFRPQLPLDQAGFKSAKPDEARRLENEFEFMFDKEWKINGRI
                     YANDDKVHRKGQEDFRFNRDGSYSGVVEGKEISGTWMGRLENNAPVITVFPTDLKEKV
                     NEWFVKNTGKTMVWSGTPSYKDNGVMIQFVLKK"
     gene            4777..6111
                     /gene="tilS"
                     /locus_tag="PLG24_06380"
     CDS             4777..6111
                     /gene="tilS"
                     /locus_tag="PLG24_06380"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HMZ24550.1"
                     /translation="MNSFLRNILQRFNKNLEVLAGNEYTRFKYLVAVSGGSDSIALSE
                     LCRMSDLDIAIAHCNFKLRGKESDEDEAFVVEFATGHQIPVFTRTFDTKLEARLLDLS
                     VQETARKLRYDWFYQLCESNQFDFILSGHHEDDRIETFVINLMRGAGPKGLRSIPPRN
                     NKILRPLLGFQREELKVFCHHAKLQFRSDSSNLDDKYSRNFIRNQVIPLVENRYPGMK
                     GSLTHVMKIQDLYFRFVETRVEKELHQRIKPNPYGMILDLNDLSEPDLLIFLLIFWMG
                     DLGFSHSDIEDMSRCLSERKSGARFFANNSMVISDRGKIVYLDREVPSITLEFPLVEG
                     KYQYHDSIFVISKSNRIPDKGEIKIPEKAKQSTIKIRARQDGDRIRSLGMEGKSQKLQ
                     DVYTNARLNYLQKLIQPVVLLDDKIIWLPGLKKSVETSTADGPGWIISHKSH"
     gene            complement(6252..>7300)
                     /locus_tag="PLG24_06385"
     CDS             complement(6252..>7300)
                     /locus_tag="PLG24_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24551.1"
                     /translation="AELAKADAAIKMIDPNVKLKNIEAPFIAFESYKMSIAKATKESA
                     KKDANNGLNELIPMLSTSGLEYFYDQNFEKAFRDFKAIIEAYDIAKSAKLKTALDKPE
                     DLNNQYYVTGLSALNAGKEAEAGEWYEKAFNGGNKGAEIYDGLFKSYQKSDPAKAEKY
                     LTEGRTAYPDDNSLLFSEINLYLSQGKLSSLIDNLELACQKEPDNISVINTLGNVYDK
                     LYQNASEKGDAQEAENYFNKAMESYNKALAKDPSNNFANYSMGTLYYNKAANYVKVMQ
                     TLSDDLSKEGMKKYDEAQKNMFGMFDAALPYFLKAEESDPKDRNTLIALREIYARKNQ
                     LDKAEEYKKKLDAL"
BASE COUNT         2181 a         1531 c         1346 g         2242 t
ORIGIN      
        1 atattactca agatccaggc gtaccagctc tcaatcttgc gcttagccat ccaccacatg
       61 gcagtagcgg ctgatgcaga aaccaaagaa tcaccaacta ctgtagtgct atccgtaaaa
      121 taaaacaaaa agaataggag cgaaatcagg attatcagga atacagcaat cccttttatc
      181 cactcattcc tatcgcaata tgaaatagga aaaacaaact cctcttcctt ttttttagac
      241 caattgaccc aacctaccac gctcatgaca aaataataaa aattgactcc ggcgtcggca
      301 tataatttat gtataaggca aatgtagacg taaagtaaaa cactgacgat tcctgtagga
      361 tatacaagaa tatcattttt cctactatac catacgctaa ctactccaaa aaaagctgca
      421 acaaattcca gggtatggct ttgaaaatac tgccaaacag cttcaagtat ttccatcacg
      481 cttgtataaa gttcaaattt tttcgtaaat tcattgtgca agatatcaat atatgagcaa
      541 tatgtcatag cattcaataa aaaatccaca ttcgcaagta ttctgaaact atattcaatc
      601 tgatcggttc aattgcatag caaaagaact tgtgaacgat gacagaaagg atgagagcgg
      661 cgattaagcc tttatttcca ttgtcgtgcg atagaaaagt ccggaaatcc agattacata
      721 atataattga attttattga aaatcaatca cacaccattt aatgccgttg catcttttca
      781 ggcggttcta tgtttagata aagtatagtt ggaaatgtgt atttcagtaa ccatcaatac
      841 ttctcaccga ttgccttccg gttgtttgaa caagatcaat ctaattactg aaataaaaag
      901 cacaatggat cgtcctgaat taattataat gaatggttat taaatgaaag attaaatttg
      961 cccttaaaca tatacaatcg atcaaaacgc atattaaatg gatacaaatg aatatcagga
     1021 ttcccgtata gataatttaa ttaatcacta caaccaaatc atcactttgt caggtgagga
     1081 tgtggagcgg gaaggacttc tcaaaacgcc caaaagagca gctaaggcaa ttgacttcct
     1141 caccagcgga tacagaatgg atgcagctga gatccttcga tcagctatgt tcaatgaacc
     1201 ttacaatgaa atgatcatcg tgaaagatat tgaagtatat tcactatgtg aacatcatat
     1261 gcttccattc tttggaaagg ctcatattgc ctatatacca aaggattata ttgtcggcct
     1321 gagtaagcta cccagagtag tggatgtatt tgcccgaaga cttcaggtac aagaaaggct
     1381 tactcaggag attctcgaag taatcgacaa cacactgaaa ccgcatggtg tagcagtcgt
     1441 aatcgaagct tctcatatgt gcatgatgat gcgtggcgtg cagaaacaga actctgtaac
     1501 aaccacttcg gcttttaccg gtgaatttaa aaatgttgag accagaaatg aatttctcca
     1561 gctaattaga taatcctcct cccttagaaa taataaacta taggaacgac tgttccgttc
     1621 aacgagtcat ataagaggta tcaattgtca atggtatatt caccattagt taacttaaca
     1681 atctgtgcac tcgggaagcc gcctgctttt accttatcaa taacctgctt gacttcttct
     1741 ttattttcga atccacttaa ataaaaagtt gtcagattgc cggttttaat ttcttcaatc
     1801 aagccatact gcctgactgc ggaaccattg aaaagggaag catttctcaa ggttgataac
     1861 cttattttgt attctaccct tgactcttcc aaggacttct ggacaacctg aggctcagaa
     1921 ttggtctttt cctgaggttt tacctccgat ttcatttcag gcttgttttc aggttgtatc
     1981 cttggatcag gttcagcctt cttctccggc ttattcatag gcgggtcaat agcattttta
     2041 cgatcaacag gtatcgcctg cctattatta tactcgcttt cttttacctc cacaataaaa
     2101 cccttgcgta attcttcggg caattgttct ctgatggcca atgcatcctg tcgtgtttca
     2161 tagaaaccaa ggcgaacttt atttaaatta ccttcccttg caatatagac ctgaccacga
     2221 cccctggttt tctcctgata gggctgaagg ttatgattgt caagagaaat agcagcaagc
     2281 tggatagcat attttacaat agcttctttc ttattttccg tcttaggtgc gactatgacc
     2341 ggagattggg caggtgcaat ctgcttattc tcctgcataa cttcttcctt gactgtctct
     2401 tctctcaatc tgttatcatt gtaatccatt actttttgag cgtcagtgac ggatgtttca
     2461 ggtcccttga cactctcaat tttttttgct actcctgttt cgacaaaatc tgaagtcgaa
     2521 ggattctctg tttcggactc atttcgctgc gtaactttaa tatcgtccat gctggaagtc
     2581 ttaatgatac cagatcgcct gctaccattg ctcaccagtt ttatggttcc cagaatatca
     2641 gccggaactg atgccaatgt aacaatcttg ctttcactag ggttataata agggcttttt
     2701 gaccgaatca tataatagga caagggttcc aaagcgatgg tgtagtagcc gttgtcatcc
     2761 gtaaaaacat attgtaattc accggttgcc tgatcgacaa tggacaattc gacctcatct
     2821 gccggattgt tatcctgatc caccacgcgg ccttgataga agactccttc ctttgaaagg
     2881 ttgatattga tggatttttt caaaccaata atttcatcga aatcgattgg aattgtagct
     2941 gaattgtatc caaccttacc tacctgaata aaacacttct cacctgcggc taccggcagg
     3001 attacactac ccttactatt cgcataaagt ggatttttac gacatcttga caaatcgacc
     3061 tttgccccgg aaattgcttt cttatcctca ttggacttga tgttcaggag gatcagtttt
     3121 ttctcattgg tcactttgaa cgcttttcca ttcactcctt cacgatcgga aaggaagtaa
     3181 cctatacggg tattgttatc aaaactcatt gaataatcat cggcaggact gttgattggg
     3241 cttccggcat tgattaccag ccgacctaaa tccttatcca tatcggcgat gaaaatatcc
     3301 atcctattgg caccgtggcc attgctagaa aaaaacagca tattatcaat catgtaagga
     3361 tacacctcat ccttttctga attgatcttc ggaccgagat ttcttggttt actccattct
     3421 tttccacggt aatcacaaac atatatgtcc aaacctccat aaccacccgg catatcagaa
     3481 gcaaaataaa gcttggtccc gtccgggcta agatagggtg tagtgcagga atacgcttca
     3541 ctattcaatt gaaactcaaa atatttggat gcaataccgt tcttgtcctg aaaagcaaaa
     3601 taaattttgt taccctttaa ttttgccggt acattctgct tattgtcgcc atatgaaatg
     3661 gcaatcaatc caccctgatc tgacttggca aaaccggtca caatggccct tctaaggctg
     3721 aacccttcga tagggatcac tttatcactc cccagtgaat atatattgac attcttgtca
     3781 tccaactgag tatataccca gtcgcctttc aggtataatg ggctgtactc caggacatcc
     3841 ctactgagac tagccttttc tactttgaaa atagtattgt cagtctggga atgggcaatt
     3901 gaaaaaatac ccatcataaa cacgaaaaga gttaagcttt gcaggttacg actaaacata
     3961 ttattttatt ttaaaatgtg tactgcaaaa ataaacagtt ttttgaatat taattatttt
     4021 tttaatacaa attgaatcat gactccgttg tccttataac tcggcgtacc ggaccaaacc
     4081 atggtcttac cggtattctt aacaaaccat tcgtttactt tttctttcaa atcagtggga
     4141 aatacagtaa tgacaggtgc attgttctcc aaacgaccca tccatgtacc ggagatttcc
     4201 ttgccttcca ccacaccgga gtagctacca tctctgttaa acctaaaatc ttcctgacct
     4261 tttctgtgta ctttatcgtc attggcataa atccgaccat tgattttcca ttccttatca
     4321 aacataaact caaattcatt ctctaatcgt ctggcttcat caggctttgc agatttaaac
     4381 cctgcctgat ccaacgggag ctggggtctg aaggcattgt catccgcctc cggatcaacc
     4441 ggcacatcac ccggttcagg aacatatttc ttaaacctca cagaatcagt gcgtggagta
     4501 gcttcgacac ttgaggtacc attatctcca ggcttacatt ggatgaatgt caaaaaaagg
     4561 caaaaagtca ataaacgcaa agtaaaatta atcatgatgt ataaaagtta gttgatcaat
     4621 caaaaaaggg gcacttctat ggttgaaatc tttttggcaa atttttactt gccggataaa
     4681 gatttggttg ggttgattca aataaaagta gtattttcaa tatttgataa tatttttgtc
     4741 aggataacgc cataaagata acttttattg gactgtatga attcatttct tcgtaatatt
     4801 ttacaacggt ttaacaaaaa tctggaagtt ctcgctggca atgaatatac ccggttcaaa
     4861 tacctggtcg ccgtaagtgg gggttcggat tccattgcct taagcgaatt gtgtagaatg
     4921 tcggaccttg atatcgctat agctcattgt aattttaagt taagaggcaa ggaaagtgat
     4981 gaggacgaag catttgttgt ggagtttgct accggccatc agattccggt atttaccagg
     5041 acatttgata cgaaactgga agcaagatta ttagaccttt cagtacagga aactgccaga
     5101 aagttgaggt acgactggtt ctaccaattg tgtgaatcaa atcaatttga ctttattctg
     5161 tccggacatc atgaggatga tcggattgag acctttgtca tcaacctgat gaggggagcc
     5221 ggcccaaaag gcttacggag tatacctccc cggaataata agatattgag gccattgctg
     5281 ggctttcaaa gggaagagtt gaaagtcttt tgtcaccatg ccaagttaca attcaggtca
     5341 gacagcagca atctggacga caagtatagc cgtaacttca taagaaatca ggtgattccc
     5401 ctggtggaaa accgataccc gggaatgaag ggcagcctga ctcatgtcat gaagattcaa
     5461 gatttgtatt ttagatttgt ggagacacgg gtagaaaaag agttgcatca aagaatcaaa
     5521 ccaaacccat atggaatgat tctggatctt aacgatctgt cggaacctga cttactaatc
     5581 ttcttgctga tattctggat gggcgatctt ggtttttctc attcggacat tgaggatatg
     5641 agcagatgtc tttcagaacg aaaaagtggt gcacgatttt ttgcaaataa cagtatggta
     5701 atcagtgacc ggggaaaaat tgtctacctg gatcgtgaag tcccttcgat aacacttgag
     5761 tttccattgg tggaaggcaa ataccaatac catgactcaa tcttcgtgat ttcaaagtct
     5821 aatcgaatac cggataaagg tgaaataaaa ataccggaaa aagcaaaaca gtcaactatt
     5881 aagatcaggg ctcgtcagga tggagaccga atccgttcct taggcatgga aggaaagagt
     5941 caaaaacttc aggatgtcta taccaatgca agattaaatt atttgcaaaa attaattcaa
     6001 ccggttgttc ttttggatga caaaatcatt tggttaccag gcctcaaaaa aagtgttgaa
     6061 acaagcactg cagatgggcc aggctggatt atttcccaca aaagtcattg aatgttgaga
     6121 caccgtgata accaatttgg gcattcaaat gagaaacggg taaaagaatt tcagacaaca
     6181 aaatgaatct tacaaaaaca gagaggggaa gaaatacatc ttcccctctc catttccttc
     6241 aaggctcctg atcaaagagc atccagtttt ttcttgtact cttctgcttt atccagttga
     6301 ttctttctgg catagatttc tcttagggca atcagggtgt ttctatcttt aggatctgat
     6361 tcctccgctt taaggaagta aggcaatgct gcatcaaaca tgccaaacat attcttttgt
     6421 gcctcgtcat acttcttcat accttcctta ctcaaatcat cacttagcgt ctgcattacc
     6481 ttgacatagt tagcggcttt gttataatac aatgtgccca tactgtaatt ggcaaagtta
     6541 tttgatggat ctttggccaa tgctttatta taactttcca tcgctttatt gaaataattc
     6601 tctgcctcct gagcatctcc tttttcactc gcattctgat aaagtttgtc atacacgttt
     6661 ccaagcgtat ttataacact gatgttgtca ggttccttct ggcaagctag ctctaaatta
     6721 tcaatcagac tactaagttt accctgagaa agataaagat tgatttcaga gaacagcaaa
     6781 ctgttatcat ccggataggc agttcttcct tccgtgagat atttttcagc ctttgcggga
     6841 tcagatttct gataggactt gaataatcca tcgtagattt cagcaccttt atttccacca
     6901 ttaaaagctt tctcatacca ttcacctgct tctgcttcct tgccggcatt gagagctgaa
     6961 agtcccgtca cataatattg attgttgaga tcttcaggct tgtccagtgc ggtcttaagt
     7021 tttgcactct ttgcgatatc ataagcctca ataattgctt tgaaatcccg aaatgccttt
     7081 tcaaagttct gatcgtaaaa atactccaga ccggatgtgc taagcattgg tatcagttca
     7141 ttcaaaccgt tattagcgtc tttctttgca ctttcctttg ttgcttttgc aatactcatt
     7201 ttatacgact caaaagcaat aaaaggggct tcaatattct ttaatttaac attggggtcg
     7261 atcattttga tggcagcatc agctttagcc aattcggcat
//
LOCUS       DAOQQN010000134         7286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4139_length_7286_cov_5.538653, whole genome shotgun sequence.
ACCESSION   DAOQQN010000134 DAOQQN010000000
VERSION     DAOQQN010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4139_length_7286_cov_5.538653"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="PLG24_06390"
     CDS             complement(<1..1228)
                     /locus_tag="PLG24_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HMZ24552.1"
                     /translation="MGLKDRIQSFFTGLYRSRFSNWITVLGLYMAFSLLIRIAFSIWS
                     AGDASYSLYNVSRAYGIGFAYDLAVGMSFLFGYAVYLILLPARWIGSVFDRVCTYGYL
                     SIMLFIMYFSLMAEVPFWEEFATRFNFIAVDYLIYTYEVVANINQSYPLPLLIGGMVA
                     IILGTFWLLKKTGARRNTFNAKSGFFRRFTIGLPTLLLTPILLTMLNNKQAEFSENVV
                     VNEIGKNGVFSFFAAFRSNELDYDAFYPKVAESEVYPVLRQDLKQLNQTYPLCLPDDV
                     MRFTKGDQTVKEDTPNIVLVVIESFSADFSGLFGNDLNLTPVYDSLAKQSVLFTNLFA
                     TGTRTVRGMEALTLCVPPTPGNSIVRRPDNDSLFSIGTILRDKKYVPYFIYGGDGYFD
                     NMNNFFGGQGFTIVDRN"
     gene            2227..3825
                     /locus_tag="PLG24_06395"
     CDS             2227..3825
                     /locus_tag="PLG24_06395"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HMZ24553.1"
                     /translation="MKHVTLFPLLIITLVILLSGCAENPRQKLVPLVDEATYSEVDFT
                     DVKIDSALVSTFLATLNTTDSIKSRVASFYRRRNYQFGWFTKTGMTYPVNIFYDQLNE
                     YINDFKDSTLVDPTLDSLIQLARKDERSFLKVRTNAPKLDLLLTTYYFKYADRAYGGL
                     DKSPVDLEWYIPRKKKNYEMLLDSLVIRKKTDKIKEPENIYYTRLIKELKRYRQIEKN
                     GGFPLIEEGKEIKKGDTSDVIARIRDYLRLTGDLAEGDTGRIFDDNLSKSIKIFQSRM
                     GLIPNGKPGKSTIDAMNVPIDRRIQQIMINLERLRWMPDRLVDNMILINIPEYVLHYY
                     KNDSLAWNTNVVVGKEITKTVIFSGNMSNIVLNPYWNVPQSIMKKEIIPAMQKNPNYL
                     DKNNMEIISKSPLSVRQKPGKNNSLGKLKFLFPNNYSIYLHDTPAKYLFSQNKRAYSH
                     GCVRVENPVTLAKLLLTDQTQWLKKVDAILKTDKETWIPVKPPMPVFIVYFTTWVDQA
                     GNLNFRNDVYGHDKKLGEEIFGKD"
     gene            4251..5174
                     /locus_tag="PLG24_06400"
     CDS             4251..5174
                     /locus_tag="PLG24_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HMZ24554.1"
                     /translation="MSLWNSYINGFQNYLRLEKGLSAHTQAAYLSDVGKLAWHIEQTG
                     TDKSPLQVEASDILDFIIEINKMGIGERSQARILSSFRSFFRYLLIEDLIQSDPVELI
                     QGPQLPTRYPEVLTIEEIEAFLAAIDLSEPLGHRNRAMFETMYACGLRVSELIHLKLA
                     GYFPDIGFVRVIGKNNKERIVPIGEIAIKQITFYLEGERRQITPKQGFENHIFLNRRG
                     AGLTRNMVFLLTKEYAQKAGITKNISPHTFRHSFATHLVEGGADLKAVQDMLGHESIT
                     TTEIYTHLDLGYLRETIFNFHPLFSGRKNQK"
     gene            complement(5204..5818)
                     /locus_tag="PLG24_06405"
     CDS             complement(5204..5818)
                     /locus_tag="PLG24_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HMZ24555.1"
                     /translation="MQEQTVKTPILIYTEETPNPDSLKFVVNIILYPGGTGDFRDVES
                     AQKWSPLAAALFEFPYVKGVYISNNFVSVTKEATYSWVEIQQSIKDFIKHYVIDDKPV
                     INEGYGEELKRLEAERIAKAYSGDTAAIVQKIKDLIETVVKPAVANDGGNIEFKSYDK
                     GVVTVIMQGSCSGCPSSTLTLKAGIEGMLKRMVPEVLEVVSEMG"
     gene            5983..>7286
                     /locus_tag="PLG24_06410"
     CDS             5983..>7286
                     /locus_tag="PLG24_06410"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HMZ24556.1"
                     /translation="MKKVFYKLLLSRLILPFCISFIISDQASGQDRSIRFLNKLTERP
                     VADVVVNCNRTGDFMSSNEAGFITLPEGTTSIWVSCIGYLTMAISTDTLKYTNDKTTI
                     LMEPTIMTLEEIIITSGMQQSVYKEISDLDIHMRPINNSQEVLRMVPGLFIGQHAGGG
                     KAEQLFLRGFDIDHGTDIQISVDGMPVNMVSHAHGQGYADLHFVIPELIDRVQFDKGP
                     YFGDHGNLATAGYVDFRTKNVLDRNFIKVEGGQFSTLRGLFGANLIRSGNEEKSLLMA
                     GEGYYTRGYFDSPQGFRRFNGLLKYNTRLNRHNDLNVTFSGFTSRWNASGQIPNRAVS
                     DGLIGFYGAIDDTEGGETSRYNASATLTSKFKDGSRWINQIYYNRYQFLLYSNFTFFL
                     RDSINGDQIRQAEKRNLTGINSKYSKQYQVGDFRLITTAGLQ"
BASE COUNT         2313 a         1549 c         1508 g         1916 t
ORIGIN      
        1 gattcctatc aacaatagta aatccctggc caccgaagaa attattcata ttgtcaaaat
       61 aaccatcccc gccataaata aaatagggaa catacttctt atcccgcaat atcgtaccga
      121 tggagaacag gctatcattg tccgggcgtc tcacaatact gttgcctggt gtaggcggaa
      181 cacacagagt cagggcctcc atgcctcgta cagtccgtgt acctgttgca aaaagattgg
      241 taaacaaaac gctctgttta gctaaactgt cgtacacagg cgtaagattc aaatcattgc
      301 caaaaagacc ggaaaaatca gcactgaagc tctcaatcac tacaagaaca atattgggcg
      361 tgtcttcctt gacagtctga tcgcccttag taaatcgcat gacatcatcg ggaagacaca
      421 gtggataggt ctgattaagc tgcttcagat cttgccgaag aacaggatac acctctgatt
      481 ctgctacttt gggatagaat gcatcataat caagctcatt ggaccggaaa gctgcaaaga
      541 aggagaatac gccattctta ccgatttcat tgacgacgac attttcggaa aactcagctt
      601 gtttattgtt gagcatcgtc aacaggattg gtgtcagtaa aagcgttggc agtccgatgg
      661 tgaatcgcct gaaaaagccg gactttgcat tgaatgtatt tctcctggca cctgtttttt
      721 tcaacagcca aaaagtcccc aggataatgg ctaccattcc cccaatgagc aggggaagtg
      781 gataggattg attgatattg gcgactactt cataggtgta gatcaggtaa tcgacagcaa
      841 taaaattgaa ccgcgtagcg aattcctccc aaaaaggcac ttccgccatc aggctgaaat
      901 acatgatgaa gagcattatc gagagataac cataggtgca gaccctatca aagacagaac
      961 caatccatcg ggcaggtaat agaatcagat atactgcata tccgaagaga aacgacatcc
     1021 ccactgccag atcatacgca aagcctatcc cataggcacg gctcacattg taaagggaat
     1081 agctggcatc gcctgcggac cagattgaaa aagcaattcg tatcaacaat gaaaatgcca
     1141 tatacaatcc caatactgtt atccaatttg agaagcggct tcgatacaaa ccggtaaaaa
     1201 atgactgtat tctatccttc aaacccatgt cgatcaatta ctattatttt aaaatcatcc
     1261 gtaaacatac cgacctttca tatgagaacc caatcaggtc gatataccct gaaaatgaat
     1321 gtaaaggtaa tcataggtaa taaaacatgt aactattgac cggatcaaaa agaatatcag
     1381 acctataaat accaaaacaa ggataaggct ttattaaaag tttagcacta atgattaagg
     1441 acatattata ggatggacaa gaatcaacct gctttccttc gtaagatgaa atcgattttt
     1501 tcaacaaaaa cctgaatatc atcttcggca tcaccggtta tgaagaatat gagatgtagg
     1561 ccgttatccg atttccgcat cttgttgata atcctttcaa tcccatcaaa atgaagacat
     1621 tggcccttag cacaggtaat ccatgcagaa ggtgcttgtc cgtaagctat cccaggtgat
     1681 gcttatacga aaggactgtg aaatattgtt tgggtatcga aactcttact gcattgtcaa
     1741 gggctgaatc tgtcatacat gaagatggcc tgaatatttc aaaacacatg actgatctcg
     1801 tgcccgggtt tgatgaaagg ggcattgatt ctgtcatcac agtttttata tcagcccatg
     1861 catgcaaatt gctattctca tcaagttgac aagtatttac cggaatgtat ttgtccacac
     1921 ccggcagggg attaaacaga aaaaacaata cacttttcag gctcgccaga caacacatta
     1981 cagcattttg atgtagaatt aaataaggac aatttgttat aaaaatgttt tgatattgaa
     2041 gacgaatgaa acatcctgaa gaaaagatca tgttgccctg aatattctat tgttgtcagg
     2101 catctttgaa aattgaatct ttgatgacat ggtgtacgaa agccatctga attgagatag
     2161 cattgttaag aacaaaaaat tcattatcat tacccggtaa aagaacattc tttaaagaaa
     2221 ccgccaatga agcatgttac ccttttccct ttattaatca tcacattggt gattttacta
     2281 tccggatgtg ctgagaatcc aaggcaaaaa ctcgttcctc tcgtggatga agcgacatat
     2341 agcgaggtag attttaccga tgtaaaaatc gacagtgcat tggtctccac ctttctggcc
     2401 acgttgaata caactgattc gatcaaaagc cgggtagcat ccttctatcg tcggaggaac
     2461 tatcagtttg gctggtttac caaaacaggc atgacctatc ccgtgaatat cttttacgac
     2521 cagctgaatg aatatatcaa tgatttcaag gactccacac ttgtcgatcc taccctggac
     2581 agcttaattc aactggcccg gaaagatgag cgttcctttt tgaaggtgag gacaaatgca
     2641 cccaaactgg accttctgct cacaacctat tacttcaaat atgcggacag ggcatatggc
     2701 ggtcttgata aaagtcctgt tgacctggaa tggtatatcc cgagaaagaa gaaaaattat
     2761 gaaatgctgc tcgactcttt ggttatcaga aaaaaaactg acaagatcaa ggaaccggaa
     2821 aacatctatt atacgcgact gatcaaagaa ttgaaaaggt accggcagat agagaaaaat
     2881 ggtggattcc cattgattga ggaaggaaag gaaataaaaa agggggacac atcagatgta
     2941 atagccagga tcagggatta tctgcgactt accggtgatt tagcagaagg agataccggt
     3001 aggatttttg atgacaacct gagcaaaagt attaaaatat tccagtccag aatgggcctt
     3061 attccaaacg gcaaacccgg aaaatctacc attgatgcga tgaatgtacc tatagatagg
     3121 cgcatccaac agatcatgat taatctggag cggctcaggt ggatgcctga cagacttgtc
     3181 gataacatga tcctgattaa tatccctgaa tatgttttgc attattataa aaatgattcc
     3241 ctggcctgga ataccaatgt ggtggtaggt aaggaaatca ccaaaacagt aatcttttcc
     3301 ggcaatatgt ccaacatcgt actgaatcca tactggaatg taccccaaag catcatgaaa
     3361 aaggaaatca tcccggctat gcagaagaat ccaaattatt tggacaaaaa caatatggaa
     3421 atcatatcaa aatcaccatt gtcagtcaga caaaagcccg gaaaaaataa ttctttgggt
     3481 aagctgaaat tccttttccc taacaattac agtatttatc tgcatgacac accggccaaa
     3541 tacctgtttt ctcagaataa aagagcatac agccatggtt gcgttcgcgt cgaaaaccct
     3601 gttactttag ccaaattgct tctgaccgac caaactcaat ggttgaaaaa ggtagatgct
     3661 atattaaaaa cggataagga aacctggata cccgtcaagc ctccaatgcc ggtattcatc
     3721 gtgtatttta cgacatgggt ggatcaagcg ggaaatctca attttcgaaa tgatgtctat
     3781 ggccatgata aaaaactggg ggaagagatt tttggtaaag attaattttt tatcgtacaa
     3841 ccaaatatac gctatcattg ccctaacaac tccgtcatcg gacaggatgt tattaaatcc
     3901 tgaaatctat tttcaatcat tcaatggaca acgcccatca atcgatattt agtaacaatc
     3961 ctaaagagct tatcagattt cccggtcttc aggcaataaa tctgccttcc cttatagtgc
     4021 tttattcttc ccaagattaa aaactcccta ttaatccgga ttgattcgat tgctgtgtgt
     4081 acaaaccgtg aaattcaccg gtggaggaca gctcttgtta cacttatgtt gcaaagaact
     4141 tccgggccga tcactacaat gccaatgaat cataatccgt tatctttcca catttcctct
     4201 acccttgatt taccaattca ttgttacatt tgccattaga ataacttgct atgtcccttt
     4261 ggaatagtta catcaatggt ttccaaaatt atctgaggct cgaaaaaggg cttagtgctc
     4321 atacccaggc agcctatctg tcggatgtcg gaaagctggc atggcatatt gaacaaacag
     4381 gcacggacaa atcgcccttg caggtcgagg caagcgacat acttgacttt atcattgaaa
     4441 tcaacaaaat gggcattggc gaacgaagtc aagcgaggat attaagcagc tttcgttcat
     4501 tttttcgata cctgttaata gaagatttaa ttcagtctga tccggtggag ctgattcagg
     4561 ggcctcaact gccgacccgg tatccggaag tattgacaat agaagagatc gaggccttcc
     4621 ttgcagccat cgacctgagt gagccattgg gacatcgcaa cagggctatg tttgagacca
     4681 tgtatgcctg tgggctacgt gtcagtgaat tgatccattt aaagttggca ggatattttc
     4741 cggatatcgg gttcgttcgt gttataggga aaaacaacaa ggaaaggatt gtacccatcg
     4801 gtgagatcgc catcaagcaa atcacctttt acctcgaggg agaaaggcgg caaataacac
     4861 caaaacaagg ttttgaaaat catatttttc tcaacaggcg aggagcaggt ctgaccagaa
     4921 atatggtttt cctgttgacc aaagaatatg cacaaaaggc aggaatcaca aagaatatca
     4981 gcccacacac ctttaggcat tctttcgcta cacatcttgt agagggtgga gcagacttaa
     5041 aggcagtcca ggatatgctg ggtcatgaaa gcatcaccac gaccgagata tatacccatc
     5101 tggatctggg ttatctccgt gagacaatat tcaattttca cccattattt tcggggcgaa
     5161 aaaatcaaaa ataaaataat aataaatcag gtcgaagata tcatcaaccc atctcactga
     5221 ctacttcgag tacttcggga accatacgct tgagcatgcc ttcgataccg gctttcagag
     5281 tcagggtaga agaagggcac ccgctgcatg agccctgcat gatgacggta accacaccct
     5341 tatcatagga tttgaattct atgtttcctc catcattggc cacggcaggc ttcacgacag
     5401 tctcgatcag gtccttgatt ttctggacga tggccgcagt atcgcctgaa tatgctttgg
     5461 caatcctttc cgcctcaagg cgtttcaatt cctccccgta accttcatta atcacaggct
     5521 tatcatcaat cacataatgc ttgataaagt ccttgatact ttgctgaatc tctacccagc
     5581 tatatgttgc ctccttggtc acggaaacaa aattattgga gatgtaaaca ccctttacat
     5641 aaggaaattc aaacaacgct gctgcaagtg gcgaccattt ttgagctgat tcaacatccc
     5701 tgaaatcacc tgttccaccc gggtacaaaa tgatatttac aacaaatttg agtgaatccg
     5761 gattgggtgt ctcctcagta tatatgagaa taggagtttt taccgtctgt tcctgcattt
     5821 ttccttcatt tacatttagg ctaataaaac acggcaaaga taataaagtt gcaggaagag
     5881 aaaaaggcct ccaaatagtg aattaccaat cttgtctgaa gaatcagaat cttcgtcaaa
     5941 gaaataagtc tattaaggtt aaatgatata tcttagcaag gtatgaaaaa agtattttac
     6001 aagcttttac tatcaaggct aatactgcct ttttgcatca gttttattat ttcagaccag
     6061 gcttccggac aagatagaag tatccgattc ttaaataagc tgacggaacg gcctgtggcg
     6121 gatgttgtcg taaattgtaa cagaaccggt gattttatga gttcaaatga ggcaggattc
     6181 atcactttgc cggaagggac aacttccatc tgggtttcct gcatcggcta tctcacaatg
     6241 gcaatcagta cggacacctt gaaatatacc aatgacaaga cgaccatcct gatggaacct
     6301 accataatga ccctggaaga gatcatcatt acctccggca tgcaacaaag tgtgtacaaa
     6361 gagatttcag acctcgatat ccatatgaga cccattaaca acagtcagga agtgctgaga
     6421 atggtacccg gcctctttat tggccaacat gccggtgggg ggaaggcaga gcagcttttc
     6481 ctcagaggtt tcgacatcga ccacggtacg gatatacaaa tcagcgtaga cggtatgcct
     6541 gtcaatatgg ttagccatgc tcacggacag ggatatgccg atctgcattt cgtaataccg
     6601 gaattgatag acagggttca gttcgacaaa ggcccttact ttggcgacca tggcaatttg
     6661 gctactgcag gctatgtgga cttcaggaca aaaaacgtgc ttgaccggaa ttttatcaaa
     6721 gtggaaggtg gacagttcag cacactacga gggcttttcg gagccaatct tattcgttcg
     6781 ggaaatgagg aaaaaagcct attgatggca ggagagggat attatacaag aggatatttt
     6841 gacagtccac agggattcag acgattcaac ggactgctta aatacaatac ccgactgaat
     6901 aggcataacg acctgaatgt caccttttcg ggctttacaa gcaggtggaa tgcaagcggt
     6961 cagataccca accgggctgt atccgatggg ttgatcggat tttacggagc tatcgacgac
     7021 acggagggcg gagagacaag caggtataat gcaagtgcaa cattgaccag taaattcaaa
     7081 gatggaagtc gatggatcaa tcagatatac tacaatcggt atcaattcct gttgtactcc
     7141 aatttcacct ttttcctccg agattcaatc aatggtgacc aaatccgaca agcagagaag
     7201 cgcaatctaa cagggatcaa cagcaagtat tcaaagcaat accaggtcgg agactttaga
     7261 cttatcacta cggcaggatt gcagat
//
LOCUS       DAOQQN010000135         7280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4144_length_7280_cov_5.803599, whole genome shotgun sequence.
ACCESSION   DAOQQN010000135 DAOQQN010000000
VERSION     DAOQQN010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7280
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4144_length_7280_cov_5.803599"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..2617)
                     /gene="pbpC"
                     /locus_tag="PLG24_06415"
     CDS             complement(248..2617)
                     /gene="pbpC"
                     /locus_tag="PLG24_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HMZ24557.1"
                     /translation="MKWRSQKLRVLIVCAVLLAAFYFILPRKLFNPSYSIILEDRNGI
                     LMGARIAADGQWRFPPEGGDDTLSQKYIDAVLMYEDRNFYRHGGVNIRSLARATWQNI
                     KARKIVSGASTVSMQVISLYRGHKARNILDKLWEIILAMRLEVRYSKEEILKLYASHA
                     PFGGNTVGYGAATWRYFGKHNKDLTWAEAALLAVLPNDPALIHPGKNRDRLRLKRDNL
                     LRKLASKEVFDPANLDLYLEEELPDRPYSMPDVAPHLLSSMQRLTGKSYMHSSVDMLL
                     QQRLNDLLANYGLALKANEVNNAAICVADVMTGEVKAYVGNMPGTGSSHGEQVDIIQS
                     SRSTGSLLKPFLAMMAIGEGKLTTRTLLADIPVNIDGFRPDNFTYDFEGAVPLDVALR
                     KSLNIPFVLLLKEYNISRFLQNLRLLGVSRLDKSADHYGLSLIIGGGESSLWELCGTY
                     ASCARILNHYNENSSRYSRHDVRPLSMEINKIEYQVKDKSPSVLDAGSIFKIFDVLSE
                     NTRPGEYEAWKTVSGPGRIAWKTGTSIGFRDAWSIGVNSRYVVGVWIGNADGEGRPGV
                     IGLETAAPLLFKVFDLLPSAPWFAKPFDGLKKVIICRNSGLAPNDTCPKDTMEVPKHA
                     DMLALCTYDRSIALDKTGSFRVSSECYSPFQMVFKNYFLLPPAQQYYYSSKHPSYNSP
                     PMWFPGCDHGIESRQSVMQFIYPNNIRKLKLPRDANGKLNPVTFVVAHKDHSVALFWH
                     LDDRYMGTTKGKHSFSTVLPSGFHTLTVVDEAGISISTSFEAVIVSNND"
     gene            3179..4444
                     /locus_tag="PLG24_06420"
     CDS             3179..4444
                     /locus_tag="PLG24_06420"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HMZ24558.1"
                     /translation="MKKLAFLFFICFSSLVSAQKSADTAQVKIPGRVNSATTLTQPYV
                     ILISSDGFRYDYLKKYNTQYLLDFAENGVWAKKGMYPTYPSITFPNHYSIATGLYPAH
                     HGIVDNKFYDPKRNEMYALGSKSILDGSWYGGEPFWSLAEKQGMMAASLFWVGSESDA
                     GGMRPSYYYKYHEKFTDDDKVRIIKGWLTLPEEVRPHFITLYFPEVDHMGHKYGPDAP
                     QTEESVHYVDTAINKLVKALQPLNLPINYIFVSDHGMIGVEKKDLVPMPAIDPEKYVV
                     VNSGTFARITAKNKADILPLYQSLKNSKPKDYRIYLAQNFPRKLHYSTREDTTRRIGD
                     IILVPKGAKSLIDVGKKTSYGKHGFDPCKVPEMKATFIAWGPAFDSPKKIKPFTNIHI
                     YPMIADILGLNVTQPIDGKLDVLKKILKK"
     gene            4826..5278
                     /locus_tag="PLG24_06425"
     CDS             4826..5278
                     /locus_tag="PLG24_06425"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMZ24559.1"
                     /translation="MKHFFIFLLFVSFLGISLQAYNHSGANLKKSIVNLKTPDADSNK
                     IYTVVSLQPEFPGWIGAMYNWINTNLKYPQKALDNQCQGKIIIQFIVERDGSLSNTKV
                     IRDGVGCDAAGETLRLIQNMPKWKPGMQDGEKVRVQYTLPVTFEIPKK"
     gene            complement(5969..6838)
                     /locus_tag="PLG24_06430"
     CDS             complement(5969..6838)
                     /locus_tag="PLG24_06430"
                     /inference="COORDINATES: protein motif:HMM:NF014708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HMZ24560.1"
                     /translation="MSLKKILANLPSDAPHMVGDGFRVHTFFPSEKISMKRMSPFLLM
                     DYNAPVEFLPREEPRGVGVHPHRGFETVTIAYKGSVAHHDSAGHAGVISEGEVQWMTA
                     GSGILHKEYHEQGFSRRGGIFHMVQIWVNLPKAFKGTDPKYQSITREDYGVAILPNDA
                     GEVEVIAGEFNGVKGPARTYSPMTLMNIRMKKGFSYHFAMPEEWNTAALMVSGEARLN
                     GMVAGKDSLTVFENDGTDIEINAAEDVTILILCGQPIDEPIAAYGPFVMNRHEELEEA
                     YRDFNLGKYGYLE"
BASE COUNT         2255 a         1576 c         1324 g         2125 t
ORIGIN      
        1 ctgctaccgc tacaacaggc accgcatgaa aggcgaaatc ctggatgaca tcatctccag
       61 aatggtcagg cataggccga tgacctgtaa gatgttaact acgggttaat aatctgaata
      121 tgaacaggat gcgcattcaa taccaactag ggcaactaag acctactgcc aaatacaaat
      181 catatttaaa aactcgttga aatctttgtc agatttgcat agatggtcag aaatactcaa
      241 ctctttttca atcattgttc gagactataa ctgcttcaaa actggtggaa attgaaatcc
      301 ccgcctcgtc aacaaccgtc aaggtatgaa aacctgatgg caaaacagtt gaaaacgaat
      361 gtttcccttt ggttgtaccc atgtatcgat catccaaatg ccaaaataat gcgacactat
      421 gatccttatg ggccaccaca aaggtaacag gattaagttt gccattcgca tcccgaggta
      481 attttaactt cctgatatta ttcggatata tgaattgcat aacactttgc cgcgattcaa
      541 ttccatgatc gcaaccaggg aaccacattg gaggactatt gtatgatgga tgcttcgaag
      601 aataataata ttgttgggca gggggtagta gaaaatagtt tttaaaaacc atttgaaaag
      661 ggctgtaaca ttctgagcta acacggaaag atccggtttt gtccaaagcg atagaacggt
      721 catacgtgca caaggcaagc atatcagcat gcttcggtac ctccatggta tcttttgggc
      781 aagtatcatt aggcgctaat ccgctgtttc tacaaataat tacttttttc aaaccatcaa
      841 aaggcttggc aaaccaaggc gcactgggca ataagtcaaa taccttaaac aatagtggag
      901 cagcagtttc aaggccaata acacccggac gaccttcacc atcggcattt ccaatccata
      961 ccccaactac atatctgcta ttaactccta tactccatgc gtctctgaaa ccaatagagg
     1021 ttccagtctt ccatgcaata cgacctggcc cggaaaccgt cttccatgct tcatactctc
     1081 ccggcctggt attctcgctt aggacgtcaa atatcttaaa tattgacccc gcatccagca
     1141 cactcgggct tttatccttc acctgatatt ctattttatt tatttccatc gagagtggtc
     1201 ggacatcatg cctactatac cggcttgaat tttcattgta gtgattaagg atacgtgcac
     1261 aactggcata agttccgcac aactcccaaa gtgacgactc ccctccccct attatcaatg
     1321 acaaaccata gtgatcagca gatttatcta agcggcttac tcccagcaac ctcagatttt
     1381 gtaaaaatcg actaatattg tattctttta gaagcaatac gaatggaata ttcagtgact
     1441 ttctcaatgc tacatccaat ggtactgccc cttcaaaatc ataagtaaaa ttgtccggac
     1501 ggaatccatc tatattgaca ggaatatctg ccaacaacgt cctggtcgtc aacttgcctt
     1561 ccccgatagc catcatagcc agaaatggct ttaataaact tcccgtactt ctacttgact
     1621 gaatgatatc cacctgctca ccatggctgc ttcccgttcc tggcatattc ccgacataag
     1681 ctttgacttc accggtcatt acatccgcta cacagattgc ggcattattg acttcattgg
     1741 ccttcagagc aaggccgtaa tttgccaaaa gatcattaag tctttgctga agcaacatat
     1801 caaccgatga atgcatgtaa cttttgccgg tcaacctttg catggaagac aacaaatgtg
     1861 gagcaacatc aggcatggaa tacggacgat caggaagctc ttcttcaaga taaagatcca
     1921 gatttgctgg atcaaatacc tcttttgatg ctaatttcct aagtaggtta tctcgcttca
     1981 gacgtaatct gtcgcggttc ttccccggat gaataagcgc cggatcattg ggtaacaccg
     2041 ccaataatgc agcctctgcc cacgtcaaat ccttattgtg cttcccaaag tatcgccagg
     2101 tggcagcccc atagcccact gtattgcctc caaagggagc atgagatgca tacaacttta
     2161 atatttcctc cttcgaatac cggacctcca acctcattgc aagaataatt tcccacagct
     2221 tatcaagtat atttcgtgct ttatgaccac gataaaggct gataacctgc atggaaacgg
     2281 ttgatgcccc tgaaacaatc ttgcgggcct tgatattctg ccacgttgct cgtgctaatg
     2341 aacgaatatt aacccctcca tgacgataaa aattcctatc ctcgtacatc agcaccgcgt
     2401 caatatactt ttgtgataaa gtatcatcac cgccctcagg aggaaatctc cattgcccgt
     2461 cagcggctat tctcgctccc atcagaatac catttcggtc ttcaagaatt atgctgtaag
     2521 aagggttgaa cagcttgcga ggcaaaataa aataaaatgc cgccaacaag acagcacata
     2581 cgatgagaac ccgcaatttt tgtgaacgcc atttcatccg aaaacaaatt taaatcaatt
     2641 ttaatccata ccggatagtt tttgtaatta tggctattgc agttcaataa caaagtcctt
     2701 tcttgtatat ttataattgc agaattaatt caagtgttag tatgaaaaaa atcctaatac
     2761 agaccggcct tatatcataa taaaatcgaa taataagcca agggagatta gtcaaaataa
     2821 ttaatgattg tgacaaaaat gaggtgatat ttagccgggg aatgattaag gaatttaccc
     2881 cggaaggcaa tggacttatt attaacctgg acacaagttt taaaccccgt ccattataat
     2941 aacatcgata agatttttat tcactttgat ttttggttta tcttttatca agaaaaaata
     3001 atgccaaata aaacaacacc tgactttctg tttagtatta aattaagggt atcatcatta
     3061 ttcatagcta ctcgaccatt cttttatccg gtaaacattt gcaatttgta ttcaacacag
     3121 ttctttccat ttgtttgaaa atcttggaag ttgttttacc tttgaaaaaa caacagccgt
     3181 gaaaaaactg gcctttttat tttttatttg tttttcatca ctggtatcgg ctcaaaaatc
     3241 cgcagataca gcacaggtga aaattcccgg gagagtcaat tctgccacaa cattgaccca
     3301 accctatgta atcctgattt cgtctgatgg attccgatat gattatttaa aaaaatacaa
     3361 tacccaatac ttgctggact ttgccgaaaa tggtgtctgg gctaagaaag gtatgtatcc
     3421 tacctatcct tccattacct ttcccaatca ttactccata gctaccggcc tttatcctgc
     3481 tcatcatgga attgtggaca acaaatttta cgatccgaaa agaaatgaaa tgtacgcgct
     3541 gggctcaaag tccatcttgg atggaagctg gtatggcgga gagccttttt ggtctctggc
     3601 agaaaaacaa ggcatgatgg ctgcaagctt gttttgggtt ggttccgaga gtgatgcggg
     3661 aggcatgagg cccagctatt actacaaata ccatgaaaaa tttacggacg atgataaagt
     3721 gagaattatc aaaggatggc tgacactgcc tgaagaggtg aggcctcatt ttatcactct
     3781 ttattttcca gaggtggatc acatgggtca caaatacggg cctgatgctc cacagaccga
     3841 ggaaagcgtc cactacgtcg atacggcaat caataaattg gtaaaagctt tacaaccatt
     3901 gaatctgcca atcaactata ttttcgtctc cgaccatgga atgataggtg tcgaaaaaaa
     3961 ggatttagtc ccaatgcctg ccatcgatcc tgaaaaatat gtagttgtca acagtggtac
     4021 ttttgcaaga ataaccgcaa aaaacaaagc tgatatcctg cctttatacc aaagtctgaa
     4081 gaacagtaaa cctaaggatt atagaattta tctggcccag aattttccaa gaaaactgca
     4141 ttatagcacg cgggaagata caaccagaag gattggagat ataattcttg ttccaaaggg
     4201 tgccaagtca ctgattgatg taggaaaaaa gacttcttat ggcaagcatg gatttgatcc
     4261 ttgcaaagtt ccggaaatga aggcaacctt catcgcctgg ggaccggcat ttgactctcc
     4321 aaagaaaatc aaacctttta ccaatattca tatctatccg atgattgccg atatccttgg
     4381 tcttaatgta acacaaccca ttgatggcaa actggatgtc ctgaagaaaa tcttaaagaa
     4441 ataaccttga tataaaataa gattagccta tacatccttt aaaacaaggc tctttctctc
     4501 aataatgatg gcacaaatta taccaggatg atttatatag tagaccataa attttgggta
     4561 taaagccgag gttacagccg catagatcaa tgaaattggt ctataacgaa tgtatctttc
     4621 ggtattattt attaatcaaa gattgaaata aattaaattt aaccctcttt gttttttata
     4681 aatattttta aaataccggc ataagccatg caaaaccctt atatttaagg acatatcaat
     4741 actttttttg gcaacaattg aactatctgc aattttaagg ggctgataaa taaatatctt
     4801 atctcaatca aaaaagccac cggtcatgaa acattttttt atttttttac tttttgtatc
     4861 atttttggga atctcgctgc aagcctataa tcacagcgga gctaatttaa aaaaatccat
     4921 agttaactta aaaactccag atgccgatag caataagata tatacagttg taagccttca
     4981 gccggaattt ccgggttgga ttggggcaat gtacaattgg attaatacca acttgaaata
     5041 tccacaaaaa gctcttgata atcaatgcca ggggaagatt atcatacaat ttattgttga
     5101 aagggacgga tccttatcca ataccaaagt aattagagat ggtgtgggtt gtgacgcagc
     5161 cggagaaaca ttacgcctaa tccaaaatat gcccaaatgg aagcccggca tgcaggatgg
     5221 tgaaaaggtt agggtccagt acactttgcc tgttacattc gaaataccta aaaagtaagt
     5281 taacatccag tttaaggatt aaaccctttt ccaatgtgca ccaactgatt gagatcgttg
     5341 gagcccgtgc ctttgtgtgc ctttttcggc aagcaagcta ctacagatta ttttggtgtc
     5401 gtaaggaaat gaaatcctga tggtataaat gtgattcatt tgtcaggttg cattattgct
     5461 cagcaggata actactggct tgtgaacaga tggatggtaa aaaattgtta ggagaaatac
     5521 agggttgcac accagggttc tatttcctta atggaatatt ttttgagcta tttgactaca
     5581 aaaaagtgat aaaattttat gccgctgtaa tctttgatac ttgtttcaat acttggggga
     5641 tgacaactac atatataaac catacattga cacccattgc tgtagatgcc gccctcaggc
     5701 gcatttcagt cacaccttga attcaagccc ttttatacca ggatgattta tataatagac
     5761 tataaatttt tggtattgga tttattttag gaattagaaa gggccggtaa ataccaatta
     5821 cttatgttta ttgaatcagc ctgatcacaa tactctaagt caaatcaaat gcactttgca
     5881 taatatccat gatcctgtac gacaaactat tattttcaat attaaaaaaa ataccaattc
     5941 cgaatgtttt ttcagatttg gatctccatc attccaaata tccgtatttg cctaaattga
     6001 agtcacggta tgcctcttcc agttcctcat gccggttcat tacaaacgga ccgtaagcag
     6061 caatgggctc gtctatgggt tgcccacaca gtataaggat ggtaacatcc tcagctgcat
     6121 tgatttcgat atcagttcca tcgttttcaa ataccgtcag actatctttt ccggctacca
     6181 tgccattaag cctggcttca cccgatacca tcagtgctgc tgtattccac tcttcgggca
     6241 tggcaaaatg atatgaaaag cccttcttca ttctaatatt catcagggtc attggtgaat
     6301 aggtcctggc aggtcctttg accccattga attcaccggc aatcacctcc acctcacctg
     6361 catcattggg aaggattgcc actccataat cttccctggt aatggattgg tatttcggat
     6421 ctgttccttt gaatgctttg ggtagattaa cccagatttg caccatatga aatatgccac
     6481 cccttctact aaagccttgc tcgtgatatt ctttgtgtag aatgccgctt ccggccgtca
     6541 tccattgtac ctcaccctcg ctgatgacac cggcatggcc ggcactatca tgatgggcca
     6601 cacttccctt ataagctatc gttacagtct caaatccacg atgaggatgg acacccacac
     6661 cgcgtggctc ttcacgtggc aagaactcaa caggagcatt atagtccatt aacaaaaatg
     6721 ggctcatccg cttcatactg attttttcac ttggaaaaaa agtgtgtacc ctgaagccgt
     6781 ctccgaccat gtgcggtgca tccgagggga gattggccaa tatttttttc aatgacatga
     6841 attgattttt atatagtaac ctttaaatct ctataatggt tacctatttc tcaggaacca
     6901 aatcttttct taaacaggat taagaaattc gctgaataaa tgtgtattta aacctgattt
     6961 tataaatatt ttaaattcac caaatctcta atgcaaaaat ttatcctgga ataaatgata
     7021 tggggcaccc actcctgaga aagaacaaaa attggctggc ctgcttacat ctcgccccct
     7081 aattctgact gtctttagct ttcttatgat aaataaataa cacgacaaca tatgccctaa
     7141 acagcagatt agagcatcta ctctaaagac atatggcttg catgatgcat ttttcaatat
     7201 ttcaccgtca atacttatct tagcccctta aaatataaca agaagatgaa aaaagtagca
     7261 gttataggtg ctggtacaat
//
LOCUS       DAOQQN010000136         7260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4161_length_7260_cov_5.966135, whole genome shotgun sequence.
ACCESSION   DAOQQN010000136 DAOQQN010000000
VERSION     DAOQQN010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7260
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4161_length_7260_cov_5.966135"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1327
                     /locus_tag="PLG24_06435"
     CDS             <1..1327
                     /locus_tag="PLG24_06435"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HMZ24561.1"
                     /translation="TINNEIPTYTSLSDCTGSVSAYFAMQPGSICGGGSVYYKDKSVN
                     GSTRTWTFAGGTPSTSTDQQPVVTYNTVGTYSALIVSGNSSYGLANAVIVGNAPALNQ
                     NACPLPTNPPGNAGLQMFALNGMTSNSGVGSDDGSIYVDRSCTNIATLEPSTTYTMSF
                     TVGNCASNPIIYENIKAYIDYNDNGEFGGGELVVSSSSNYYCGTVNFNFTTPASPIMN
                     KLLRLRVITSPSGVSGPCQNITNGQVEDFSIIFKQNIPLPLNLLSFTGRNEGTVNILQ
                     WSGIDERNVREYLVERSRDGKLFSPIGFMTAQNKPSVSYTFTDISAETNHLQYYRLMI
                     IDQSGNVSFSKIVTIDAGKRSFTLDRLIRLIPAGGKLSGNIICQKQGKIEIGLFDMLG
                     KPLVLKTITVGIGVEKLEINMADFAAGTYILTLRDESGTVITEKVVKGE"
     gene            1999..3234
                     /gene="odhB"
                     /locus_tag="PLG24_06440"
     CDS             1999..3234
                     /gene="odhB"
                     /locus_tag="PLG24_06440"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HMZ24562.1"
                     /translation="MSIVEMRVPTIGESINEVTLSQWLKADGAIINMDEPICEFESDK
                     ATLEFPAEAAGKLIYVAGEGDDLKIGDLVAKIDTSFAGSTTETKPVETSVTAEEKHAK
                     PEPATSNYATGTPSPAAGKILSEKGIDPGSVTGTGKDGRITKDDAQKALNQSVQPSRA
                     EEQKSVAATESNQTTAFSRNERREKMSRMRRTIANRLVSAKNNTAMLTTFNEVDLTNI
                     NAMRAKYQDAFVAKYGIKLGYMSLFAKACAAVLLEMPEINAAIDGEDVIYHDYADISI
                     AVSTPTGLVVPPVHNVESLNLHEIEFKIKELAEKARNNKLTLEEMRGGTFTITNGGIF
                     GSMMSTPILNEPQSAILGMHAIINRPIAVGNQVEIRPMMYLAMSYDHRIIDGSSSVTF
                     LVRVKKLLEDPTLLFLGLV"
     gene            complement(3521..4780)
                     /locus_tag="PLG24_06445"
     CDS             complement(3521..4780)
                     /locus_tag="PLG24_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24563.1"
                     /translation="MKRITLAGCIMVLTVSCFAQKNLKDTINEKEQQVSEVQNDNQNL
                     NQFKVSGYIQAQIQSGSKDATLRVGNKNTDPDHRFNRFGIRRGRVKLTYTKNIVSGVF
                     QLDITERAISLKDAYISIQDPHWGIYSIRAGVFNRPFGYEVAYSSSRRESPERALVNT
                     ILFPNERDLGAMLTLQGPKASMWNFFKLEAAIIAGNSIRIDMDNRKDFLGRVSFNKAF
                     GEKINLSGGLSYYLGGVYQGSYNVYTMKGRSFLLSHDTTNLGAFTKREYCGVDLQFEA
                     TTRLGMTRLYGEFLTGVQPGDEQDSQSPNYSKLPTTDTYIRPFRGGYITFVQDVGHMP
                     LAVIVKYDIYDPNTDVADNDIGLNGTGTGDVAFENIGCGLMWHIRDDLKLTGYYDHII
                     NEKTSNLNAYVQDMEDDVFTLRLQYKF"
     gene            complement(5491..5850)
                     /locus_tag="PLG24_06450"
     CDS             complement(5491..5850)
                     /locus_tag="PLG24_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24564.1"
                     /translation="MVLPGEISKEIRLMPHNDWSVLHFPDHAESMEDDVRFHFRDPEE
                     LKYADPEDIVEEDNGFPLHISLTCIALYLFATGMIFLLVQNWNQDRTPYFVRIFISII
                     SATYLIFRFHKRPVLKS"
     gene            complement(6006..6076)
                     /locus_tag="PLG24_06455"
     tRNA            complement(6006..6076)
                     /locus_tag="PLG24_06455"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6042..6044),aa:Cys,seq:gca)
     gene            6240..>7260
                     /locus_tag="PLG24_06460"
     CDS             6240..>7260
                     /locus_tag="PLG24_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HMZ24565.1"
                     /translation="MTNDRLIQKYIFLFLIVGLGVTIVSNLSAFITAALGASIFYILF
                     RRQMSRLVEKFKIGKTVSALIIILLSFFIVVLPFTMMGLLIANKVIYYINQPQAFNNI
                     LDLVLEKIKLLPFEIKVGEMISRMTSLVGSLLTGVLNNIFGVLTTIVVMYFLLYYFLV
                     NYGKLEKSLVRYLPMRENNLKLLGIELHNMTFINAVGVPIIAVVQGLIAFFLYLYAGV
                     EDAGLWAILTGAASIIPLVGTGIIWVPITVILLAMGKITEGLVVGIGCLIVLGNVDNL
                     IRMYVSSKMGDVHPVVTFLGVFMGLNIFGLPGLVFGPLIISYFLIFIKMFKAEYGTIE
                     SRIITD"
BASE COUNT         2176 a         1513 c         1542 g         2029 t
ORIGIN      
        1 aacgataaat aatgaaatac ccacctatac tagcctgtct gattgcacgg gcagtgtaag
       61 tgcatacttt gcgatgcagc cgggatcaat ctgtggaggc ggatcggtat actataagga
      121 taagtccgtc aatgggagca cccgaacctg gacctttgca ggtggaaccc catcgaccag
      181 cactgatcaa caacccgttg taacctacaa tacagtcgga acctattctg cattgatagt
      241 gtcgggcaat agctcctatg gtttagccaa tgctgttata gtcggaaatg ctccggcttt
      301 gaatcaaaat gcatgtccct tgccaaccaa tcctcccggc aatgccggat tacagatgtt
      361 tgcactcaat gggatgacga gtaacagtgg tgtcggttca gatgatggca gtatttatgt
      421 ggatcgttca tgtaccaata ttgctaccct ggaaccttcc accacctata cgatgtcatt
      481 tactgttgga aattgtgcca gtaatcccat tatttatgaa aatattaagg cttatattga
      541 ttacaatgat aacggagaat ttggtggggg agaattagtg gtttccagtt cttcaaatta
      601 ctattgtggc acagtgaatt ttaactttac aacacccgca agtccaatta tgaataaact
      661 tttaagactc agggtgatta caagcccgtc cggcgtttca ggaccgtgcc aaaacatcac
      721 caatggtcag gtcgaggact tttccatcat atttaaacaa aatattccgc taccactaaa
      781 cctgctgtca tttaccggca gaaatgaagg aacagtcaat atacttcaat ggtcaggtat
      841 tgatgaaaga aatgtccggg aatatcttgt cgagagaagt cgggatggta agctcttcag
      901 cccaatcggc ttcatgacgg cccaaaataa accatctgta tcctatactt ttacagatat
      961 atctgccgaa acaaatcact tgcagtatta tcgtctgatg atcatcgatc aatcgggcaa
     1021 cgtctccttc agcaagattg tgacaataga cgccggaaag cgtagcttca ccctcgatcg
     1081 cttgattagg ttgattccgg caggtggcaa gttgtccggg aatattatat gtcaaaaaca
     1141 aggaaaaata gaaatcggct tgtttgacat gttgggcaaa cctttggtgc tcaaaaccat
     1201 cactgtagga ataggggtcg aaaaacttga aataaatatg gccgattttg ctgccgggac
     1261 atatattctt actctgagag atgagtcggg tacggtgatt acggaaaaag tagttaaggg
     1321 agaataaaag cattacaaga tacgcccctt gaagaaaata cggtgggatg taagcctgaa
     1381 aaaagttttc cggaagaaac cggacttcga tagaagatgc aggagccggg aagatttatg
     1441 tccctataag ggaaggtatt gctactgaat gaagctattg aaagggaacg ataactatat
     1501 ctatgcctgc atgtgactca tacccttgat cagaaaggag cagtgctcac ctgtcaaaac
     1561 ttcacaaacg atattacaac ctgtccggga ttggaagata ctccatcagt caaaattttc
     1621 gatacaggca tttgaaaaat gaaaaccgtt gagccttaaa agaaattaat gtgttggttt
     1681 gagtcgctat gaaggattta aatttgcatg attgacaggc ataaggggtc atagttagca
     1741 aggaggttga taaaactata aataaaagct gctaatgtgg agtcagaatg ctgaccggaa
     1801 aggaacttcc gaccagcatt ttgatgtgca agaatttgtt ttacttcaga aaaaacgcat
     1861 taatgcatcc ctttttatcg attccttaaa acttccccga tattcttttg tttgagatgg
     1921 aataatgttc taacttagcg tgttagacaa aatttaacat gcttaaaaaa atatttagat
     1981 caatctaaaa aatcaactat gagtatcgtc gaaatgcgtg tgcccaccat cggagaatcc
     2041 atcaatgaag taacactctc ccaatggctt aaggcagatg gtgccatcat caacatggat
     2101 gaaccaatat gtgaattcga gtcagacaag gcaacactgg agttccctgc agaggcagcc
     2161 ggaaaattga tttatgttgc cggggagggt gacgacctga agataggcga ccttgtagcg
     2221 aagatagaca cttcttttgc aggaagtacg actgaaacca aacctgtcga gacttcggtt
     2281 actgctgaag aaaaacatgc aaagccggaa cctgcaactt ctaattatgc aaccggaacg
     2341 ccctcaccgg cagcaggcaa gatcctttca gaaaaaggca tcgaccccgg atccgtaaca
     2401 ggtacaggaa aagatggcag gattaccaag gatgatgctc agaaagctct aaaccaatca
     2461 gtacagccat ccagggcaga agaacaaaaa tccgtagcag cgactgaatc aaaccaaaca
     2521 accgctttct cccgcaatga acgcagggaa aagatgagcc gcatgcgtcg aaccatagcc
     2581 aaccgcctgg tttccgccaa gaacaacacg gccatgctaa ccaccttcaa tgaggtagac
     2641 ctgaccaata tcaatgcgat gagggccaaa tatcaggatg cattcgttgc aaaatatggc
     2701 atcaagctcg gctacatgtc cttgtttgcc aaggcttgtg ccgcagtttt gcttgaaatg
     2761 ccggaaatca acgccgccat cgatggtgaa gatgtcatct accacgatta tgccgatata
     2821 tctatcgcag tttccacacc gacaggtctg gtagtgccgc ccgtacataa tgtggagagc
     2881 ctgaatctgc acgaaataga atttaaaatt aaggaacttg cagagaaggc acgcaacaac
     2941 aaactgacat tggaagaaat gcgcggaggt acatttacaa ttaccaacgg cggaatcttc
     3001 ggaagtatga tgagcacccc aatcctcaat gaaccacaat cagcaatcct gggtatgcat
     3061 gccatcatca acaggcctat tgcagtaggc aatcaggtag agatcaggcc gatgatgtat
     3121 cttgccatgt cctatgacca caggatcatt gacggttcca gctcagtaac ctttctggtg
     3181 agagtgaaga agctcctcga agatccgact ctgctgttcc tgggattggt ataagaccat
     3241 ggcatgtggt catttctcag tgccacagca agcgggattt ttctttcaat actgaagtaa
     3301 tcaaagacac gaaatggaaa taatcatttc atgtcttaaa gttgccaaaa attaaaaggt
     3361 tctttcctta aggaatccct gagatgttat accaggagga tttaaataat agtccataaa
     3421 tttttggtat acttgtcccg agagctaagc gattcgggga tgccgaggtt acagtcgtat
     3481 agatcaatga aattggtcta taacgaatgt accttcggta ttaaaatttg tattgaaggc
     3541 gtagggtaaa cacatcatct tccatatctt gaacatacgc atttagattg gaagtctttt
     3601 cgttgatgat gtgatcataa tatccagtca gcttcaagtc atcgcggatg tgccacataa
     3661 ggccgcaacc aatgttctca aaagccacat ctcccgtacc agtgccattc aggccgatgt
     3721 cattgtcggc aacatcagtg ttggggtcgt aaatatcata tttgacaatc actgcaagcg
     3781 gcatgtgccc cacatcctgt acaaatgtga tataaccacc ccggaaaggt cttatgtagg
     3841 tatcggtggt aggcaatttt gaataattcg gactttggga atcttgttca tctcccggtt
     3901 gtacacctgt gagaaactca ccatacaacc ttgtcatacc gagcctggtg gtagcttcaa
     3961 actgtagatc cacaccacag tattcccttt tggtaaaggc tccaaggttg gtggtatcat
     4021 gactcagcag aaatgagcgc cctttcatgg tatacacatt gtagctacct tgatataccc
     4081 cacccaaata ataggacaag cctccactca ggttgatctt ttcgccgaag gctttattaa
     4141 aggacaccct ccctaaaaaa tccttccgat tatccatgtc gatccgtatg ctgttgcccg
     4201 cgattattgc tgcctccagt ttgaaaaaat tccacataga agccttgggt ccttgaaggg
     4261 tcagcatcgc ccccagatct ctttcgttag gaaacaggat tgtgttgaca agtgcccttt
     4321 ccggagattc ccgcctggaa gaagaatagg caacctcata cccgaagggc cgattgaata
     4381 caccggccct gattgaataa atgccccaat gaggatcttg tatggatata tatgcatcct
     4441 tcaatgagat tgccctttca gtgatgtcaa gctgaaagac accggaaaca atgttttttg
     4501 tataggtcaa tttaactctg cctcttctga tgccaaaacg attaaaacga tgatcaggat
     4561 ctgtattttt attgcctacc cgaagcgttg cgtccttgct tccgctttgt atttgtgcct
     4621 ggatgtatcc ggaaaccttg aactgattta aattttgatt atcattctgc acttctgata
     4681 cctgctgctc tttttcattg attgtgtctt ttaaattctt ttgagcgaag cacgagacag
     4741 taagcaccat tatacaaccg gccaaagtaa ttctcttcat cagaatataa atttgattgt
     4801 atgcaaagat tggaatacga tatgaccaca tgatccaagg atggttacga ttagtttaaa
     4861 gtcgaggcga aaatattcaa ttactttaaa atcttaaagt tacaaatttg ctttgatgtc
     4921 tattgacaaa gttttatgat caaattttaa tataatcttg ccggtatttt ccttgtaaaa
     4981 atgcttgaat attggcattc aatccattgc gttcctttct gatgataaag gtgacttgcc
     5041 tgcagagatt gaaaaccagg gtaatgaagg atcctattta tcggtcgaac ctttaaaata
     5101 aaagaccaaa tcatgagtca ctttgatgaa ttcaatgcct gtcaagttga gacaaataac
     5161 aggaagtgac aaagtctgag tgacaggaaa accgggctga cctgcgtgaa cattcatttg
     5221 gagtattgaa ccacccaaac ggttttccac aataagatag caattatcat tcgttggatt
     5281 ttcaaaagcc gaatagcgtt atgcctggtc atttccgaaa cgtgagccta tgatataaaa
     5341 gatgtgatgt tgaatccggc acataacgat tggaatagct atctccggct atcagatatt
     5401 cagttgaatt aacttctcat gaatattgca ttgaacgtgc ctgaaaagaa ctatcctgac
     5461 tagcagaaag agaaacatta cgaatctgaa tcaggatttc aaaacaggtc gcttgtggaa
     5521 cctaaaaatc agataagtcg ctgaaattat agatatgaaa attctgacaa aataaggtgt
     5581 cctgtcctga ttccaatttt ggaccaaaag aaatatcata cctgttgcaa ataggtacaa
     5641 cgcaatacag gtcaggctta tatgcaatgg aaaaccattg tcttcttcca cgatgtcttc
     5701 cggatcggca tatttgagtt cttccggatc ccggaaatga aacctgacat cgtcttccat
     5761 agattcagca tgatccggaa aatgcaaaac agaccagtca ttgtgaggca taagcctgat
     5821 ttcttttgaa atttctcccg gcaaaaccat ggttggaaaa aagaattaaa gttgaacatt
     5881 catttaaaat cccattggta gttttcaaaa aaatattcag aaggccttgt ctcaattacg
     5941 gtaaggacca caattcagtt cttactgtat tccaatcaaa taagggggag tatatcaaaa
     6001 gaaagaggtc ggtggcgggt tcgaaccgcc gtagaaggtt ttgcagacct ttgcctaacc
     6061 gctcggccaa ccgaccctgt ttcgtaagcg gtgcaaatat aattcttttc aaacaaatac
     6121 caagagaggc tgttcatatt ttcaaatttt taaataaata aaagtatgat atttactgat
     6181 tgccaaatat ttaaaatcca tcacaatttt tatacatttg attcttaaat gaattaaaca
     6241 tgacgaacga caggctcatc caaaaataca ttttcctatt tctgatcgtt gggctcgggg
     6301 tgacaattgt gtcaaacctg tccgctttca taacagcagc cttaggtgca agtatctttt
     6361 atattctatt tagacggcag atgagccgtc tggtggaaaa attcaagatc ggcaagactg
     6421 tttcagccct tattatcatt cttttatcat tttttattgt agtcctgcca tttacgatga
     6481 tgggtcttct gattgccaac aaagtcatct attatatcaa tcaacctcag gccttcaaca
     6541 atatcctgga tttagtcctc gagaagatca agttgttgcc gtttgaaatc aaggttggcg
     6601 agatgatctc gcggatgact tcattggtag gctcattgtt gacaggcgtc cttaacaaca
     6661 tattcggtgt acttacaacc atcgtggtca tgtatttcct cttgtattat ttcctggtca
     6721 attatggcaa actggagaaa tcgcttgtca ggtatttgcc tatgcgggag aataatctga
     6781 aattattggg catcgaatta cacaacatga cttttatcaa tgccgtgggg gttccaatca
     6841 ttgctgtcgt ccagggcttg attgccttct tcttatacct gtatgccgga gttgaagatg
     6901 ccggtctttg ggctatactt acaggagcgg cctccatcat tccattggta ggtaccggta
     6961 tcatttgggt tcccatcacc gtcatacttc tcgctatggg aaagatcacc gaaggccttg
     7021 tggtcggcat cggatgcctt atcgtccttg gcaatgtgga taatcttatc aggatgtacg
     7081 tttccagcaa gatgggcgat gttcatccag ttgtgacttt tttgggtgtt ttcatggggc
     7141 tgaatatatt cgggctgccc ggactggttt tcggcccgtt gattatttcc tattttttga
     7201 tattcatcaa aatgttcaaa gcggagtatg gcactattga gtcccgtata attactgatc
//
LOCUS       DAOQQN010000137         7260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4162_length_7260_cov_5.399722, whole genome shotgun sequence.
ACCESSION   DAOQQN010000137 DAOQQN010000000
VERSION     DAOQQN010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7260
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4162_length_7260_cov_5.399722"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1068)
                     /locus_tag="PLG24_06465"
     CDS             complement(160..1068)
                     /locus_tag="PLG24_06465"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HMZ24566.1"
                     /translation="MRNNLYPFVISLVLIGVIQLIGINKNNYTNDIFIEKNTNNPIRK
                     ELIAYTANFNPALMDSIAYGRIDEASDTLDQVGPVIVTEELYKRFFRPQVEDGSGIGS
                     EMIDSAVIGAFGKLAVSGGKTVYIQLKNFGNPAYTSFMSVMEKLAGNLGIDTLYLDLR
                     NCGNGVDIEVAKIANQFFFQKGVLMMEANFFNGSKDIYNSTGKVFFPVKHIYVLINEK
                     VSGVAILLARILKNNGITTIVGDVKHTIPPVVRHFPLSDGNYFRMPVGKYSFSVEKEF
                     LDEQSSELNPDLIMGIEGINRLLGKR"
     gene            1178..1666
                     /locus_tag="PLG24_06470"
     CDS             1178..1666
                     /locus_tag="PLG24_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HMZ24567.1"
                     /translation="MNKDHDLDRIDKQILSILMKDANSTYAEIASQLEVSGGTIHVRM
                     KKLEQIGVIKAMHLIADHTKLGFEIKAFVGVSFSEGTDYKAVIEAFRKIPEITSVNLT
                     TGRFSAIVQLICKNNFHLKNILHGKIINIKGIANTETLISLDEGLSRQIDVIEIEKNK
                     DY"
     gene            complement(1837..2439)
                     /locus_tag="PLG24_06475"
     CDS             complement(1837..2439)
                     /locus_tag="PLG24_06475"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HMZ24568.1"
                     /translation="MEITQTIDKPEGKYRHMGRFAFFIESLKNIRTVGTVTRSSRFLC
                     QSVVRHADLKKAHVVVELGAGDGVMTRHLLKAMSKDSYLISFEINKVFCERLSKINDS
                     RLIIINDSAETLAQVIESRGLDKVDVVVSALPFSVFPEDLTKAIVGQCHALMRAGGRF
                     VQIHYSLKTKEIYRNIFGNVGSAFEVRNIPPAYVLYCFKT"
     gene            complement(2439..4535)
                     /locus_tag="PLG24_06480"
     CDS             complement(2439..4535)
                     /locus_tag="PLG24_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24569.1"
                     /translation="MGSFMSFGQKASLKLKDDADRSFKNKDYQKAVTLYRKYLGSGGD
                     KNIYKNLGISLYYNNQLQEAESFLAEYYNIKPNDSEVVYYLGKVFHHEMKFEKAIEFN
                     KYYLSLTKPSGPRYQQVVADVKRCGNAKNIRYQEQEAISENSGREVNTTYDEFNPVLS
                     PNFFGRVYYTSNQPVDTGRGSEVRLLPPSYNMFGADVQIGSGLMGRGSLLNINLTTRQ
                     HEVLYGFNKDGRVLYFGRGDDLGQLSIMAENTNFDPEDSSRIYKLDATFSKLGEARDV
                     FIFSDSVLIFSSDMAGGVGGYDLYYSEYKNGKWGEPINLGDRVNSPFDERSPFLAKDG
                     RTLYFSSNNYNTIGGFDVFTTYYLDKNMEWSDPVNVGLPINSPGDELYFKLTEDGLKA
                     VFSSDRKSGFGGFDLYNAFFKSVRKEQLVEALPSVFFQVPDFKLNSQEYRDEINQSKI
                     VALTLEPMYYTDDASILQAKNKKQLDQLVELAERFPSVTFNFEINSESNVSSEIELYF
                     GIKRGEIISNYMISRGVKGGRITIQSLGNLYPIARNIVDGKPSISGQNLNRRIEVRLS
                     HLDSLPLKVTYKQPFVSDLLKAGDGARLRQRTNGLSYRVQFISLRQMYTGGIYSLNPD
                     LLIESLGGSGLYRYMSGLFDNYADAKAFKDILINRGLLDSFVVPYIDNERYAPETLTE
                     AMMNKYPDLRKYFLDL"
     gene            complement(4582..5922)
                     /locus_tag="PLG24_06485"
     CDS             complement(4582..5922)
                     /locus_tag="PLG24_06485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HMZ24570.1"
                     /translation="MALYELVMPKMGESIMEATILNWAKNVGDHIDIDETILEIATDK
                     VDSEIPSPVEGTLSKILFNVNDVVPIGTVIATIETGAPVGSAVTDESASSEEHSASKV
                     PFVPEEGTKQSITVSLGDGARFYSPLVRNIASKEGISITELDAIKGTGQDGRVTKHDL
                     LDYIKVKAAIPATQVHSQQPVNPSTPVAQVESKPQAQGTPKSSGSTTSLDGDVEIIEM
                     DRMRRLIADHMVLSKHTSPHVTSFVEVDVTSMVRWREKVKKEFNQKYNQNITFTPIFI
                     EAIVKAIHDYPMINASVDGSNIIRKKNINIGMAAALPSGNLIVPVIKNADEMNLIGLT
                     KSVNDLASRARNNQLKPEEIQGGTFTLTNVGSFGNVMGTPIINQPQVAIMAAGAIKKK
                     PAVLETPDGDVIAIRHMMFLSLSYDHRVVDGSLGGSFLRRVADYLEAFDGNQTI"
     gene            complement(6619..>7260)
                     /locus_tag="PLG24_06490"
     CDS             complement(6619..>7260)
                     /locus_tag="PLG24_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24571.1"
                     /translation="IIFKPINNILIGAAIHTPSFISVTDNFSTDLRYVFTFNSEKKNN
                     YKESPEGTIDYKVTTPMRLLGSIGGIIGKFGFVNAEVEYQDYSGSKIRIDSDNLYDKQ
                     VEEQINGEIKANYTSTVKVNLGIEVAAIEHFRIRAGVGFAQTPFVGQRNFYPNFGLGL
                     GYRSDVFFLDLGFRQWRSENGYQPYYITQASQTLVNNKLTNSAVLLTIGFKLR"
BASE COUNT         2218 a         1653 c         1222 g         2167 t
ORIGIN      
        1 taacaaagcc cggaatgaat tatcaacact tgattcaacg ctattaaaca tctacctact
       61 tcagggtggc cacccccacc aaaccctaaa actcaaatct cttagttgat gaaacctttt
      121 cccccattta aagatacccc cctgatctta caaatacatc taccgcttcc ccaagagccg
      181 attgatccct tctatcccca tgatcaaatc tggattcaat tccgatgatt gttcgtccaa
      241 aaattctttc tcaacactga atgaatattt gcctaccggc atcctgaaat aattgccatc
      301 cgacaacggg aagtgcctga ccaccggagg tattgtgtgt tttacatccc ccacaatggt
      361 cgttatgccg ttgttcttga ggatgcgtgc caaaaggatt gccactccgg ataccttctc
      421 attgattagg acatatatat gtttgaccgg aaaaaatacc tttcctgttg aattgtaaat
      481 atccttgctt ccattgaaaa agttagcttc catcatcaac acccccttct gaaagaagaa
      541 ttggttcgca attttagcaa cttcaatatc cacaccattt ccacaatttc ttaaatcgag
      601 ataaagggta tctattccca aatttccggc gagtttctcc atcacactca taaatgacgt
      661 gtacgcagga ttcccgaaat tcttcaactg aatataaacc gtcttccctc ccgatacagc
      721 aagtttccca aatgccccaa tcacagcgga atcaatcatt tcactcccga taccactgcc
      781 atcctccacc tgaggcctga aaaatctctt gtacaactcc tccgtaacga taacaggacc
      841 aacctgatca agcgtatcac ttgcttcatc aatccgtccg taagctatcg aatccatcag
      901 ggccggattg aaattcgctg tatatgcaat caattccttc cgaatcgggt tgtttgtatt
      961 tttttcaata aaaatatcat ttgtataatt atttttatta atcccaatca attgtatcac
     1021 cccaatcaaa acaagagaaa tgacaaatgg atacaaatta ttccgcataa tgattaaact
     1081 tattatacca ataatagata tttattaaac atttttagta tattcgcgtt ttaatcaagc
     1141 aaaaataatc tatttaatgt tataaaattg tttaaatatg aacaaagatc atgatttgga
     1201 tcgaatagac aagcagattt tgtccatttt aatgaaagat gcaaattcaa cctatgccga
     1261 aattgcaagt caattggaag tatcaggggg tactatccat gtcaggatga aaaagttgga
     1321 acagatcggt gtcatcaaag caatgcacct aattgccgac cacaccaaac tgggatttga
     1381 aatcaaggcc ttcgtaggtg tcagcttttc cgaaggtacg gactacaaag cagtaataga
     1441 ggctttcagg aagatacccg aaataacgtc cgtcaacctg actacaggta ggttcagtgc
     1501 cattgtgcaa ttgatttgta aaaacaattt ccatttaaaa aatatcctac acggcaagat
     1561 cattaatatt aaaggaatag ccaatacaga aacactgata tccctggatg aaggtcttag
     1621 caggcaaatt gatgtgattg aaattgaaaa aaataaggat tattaatcaa acctactcaa
     1681 ccagggtttc ttaccttctt tttcctttgt agcctggaat atccaacaac tccggcctaa
     1741 ttcaagttgt gtaaaggaaa tgctctctct aattaattga taagaatacg aaaaaaccaa
     1801 tctttcaaat tctttgataa atctaggata aagcatctat gttttaaaac agtataatac
     1861 gtaagccggt ggtatattcc gtacctcaaa agccgatcct acattaccaa aaatatttcg
     1921 gtaaatctct ttggttttta agctatagtg aatttgaaca aatctacctc ccgccctcat
     1981 caaagcatga cactggccaa ctattgcctt ggtaagatct tccggaaaaa ccgaaaaagg
     2041 cagggccgat accacgacat ctactttatc caggcctcgc gactcaatca cctgagcaag
     2101 cgtctcagca gaatcattga tgatgataag ccgggaatca tttatcttac ttagcctttc
     2161 acaaaaaacc ttatttattt caaaggatat caaatacgaa tcttttgaca tcgccttaag
     2221 caagtgtctt gtcatcactc catcccctgc accaagttca acaacaacat gagctttttt
     2281 caggtctgca tgtctgacaa ccgactgaca caaaaaacgt gaactccgag tgacagtccc
     2341 tacagtcctg atatttttca gactttcaat aaaaaaagca aaacggccca tatgtcggta
     2401 tttcccttcg ggtttatcta ttgtttgagt tatctccatt aaagatcaag aaaatatttt
     2461 cgcaaatcgg gatacttatt catcattgcc tcagtcaatg tttcaggggc ataacgctca
     2521 ttatcaatat agggtacaac gaatgagtct aataaaccac ggtttatgag tatgtccttg
     2581 aatgcctttg catcggcata gttatcaaat aagccggaca tatacctgta caatccacta
     2641 ccaccaagac tttctataag taaatcagga ttgagggaat aaattccccc cgtgtacatc
     2701 tgacgcaatg aaataaattg taccctgtat gaaagcccat tggttcgctg ccgcaatctt
     2761 gcaccatcac ctgcttttaa caagtcactg acgaaaggtt gcttataggt gactttcaaa
     2821 ggcaatgaat ccaaatgact caaccgcact tcaattcttc tgttgaggtt ttgacctgaa
     2881 atactcggct tcccatctac tatatttctg gctatcggat acagattgcc cagactttga
     2941 attgtaatcc tgcctccttt aacacctctt gaaatcatat aattactgat gatttctcca
     3001 cgcttaatac cgaaataaag ttctatttcg gaagaaacat tcgattcgct attgatttca
     3061 aaattaaatg taacagaggg aaatctttca gccaattcta ccagctgatc taactgtttt
     3121 ttattcttgg cctgcaggat tgatgcatcg tccgtataat acatgggttc gagggttaat
     3181 gcaacaatct tgctttgatt gatctcgtcc cgatactcct gactattcaa tttaaaatcg
     3241 ggcacttgaa aaaagaccga aggaagtgcc tctaccaact gttccttcct gaccgattta
     3301 aaaaaggcat tatacaagtc aaatcctccg aatcccgatt ttcgatcaga actaaatacc
     3361 gctttcaaac catcctcagt caacttgaaa tacaactcat ctcccgggga gtttatgggg
     3421 agacccacat tgacagggtc gctccattcc atatttttat ccaggtagta ggtagtaaat
     3481 acatcaaaac ctcctattgt attataatta ttactcgaaa aatacaaggt ccggccatct
     3541 ttggccaaga aaggacttct ttcatcaaat ggagaattca ccctgtctcc caaattgatt
     3601 ggttcacccc atttgccatt tttatactct gaataataca aatcatagcc tccaaccccc
     3661 ccggccatgt cagaactgaa tatcagaact gaatcagaaa aaataaatac atcccttgcc
     3721 tctcctaact tcgaaaatgt tgcatccaac ttatagatcc gactcgaatc ctcagggtca
     3781 aaattggtat tttccgccat tatactaagc tgccccaaat catcgcccct gccaaaatac
     3841 aacacacgac catccttatt aaaaccatac aatacctcat gttgtcgggt agtgagattt
     3901 atattcaaaa ggctccctct tcccattagg ccacttccaa tctgaacatc tgctccaaac
     3961 atattataac ttgggggcag caagcgcact tcactacctc tacccgtatc tacaggctga
     4021 ttggatgtat aatagacccg cccgaaaaaa ttgggactta gcacaggatt gaattcatca
     4081 taggttgtat ttacttcgcg ccctgaattc tcactgatag cttcctgttc ctgatatctt
     4141 atattctttg cattcccaca ccgcttcaca tcggcaacca cctgctgata ccggggaccg
     4201 gatggcttag ttagggacag ataatattta ttaaattcaa tggctttttc aaatttcatt
     4261 tcatgatgaa aaacctttcc aaggtaatat acgacctcgc tatcatttgg cttaatgttg
     4321 taatattctg ccaaaaagga ttcagcttct tgtaattgat tattgtaata caaggaaatg
     4381 cccaggtttt tgtaaatatt cttatcgcct ccacttccta aatatttcct atagagcgtg
     4441 acagcttttt gataatcttt attcttgaag ctcctgtcgg catcatcctt cagttttaaa
     4501 gaagcttttt gcccaaagga cataaaacta cccatgagta gaaagattat agagcacaat
     4561 agcttaatcc gccccattaa atcaaattgt ctggtttccg tcaaaagcct caaggtagtc
     4621 tgccactctt cgtaaaaaag agccccccaa acttccgtct acgactctgt gatcatagga
     4681 aagggacagg aacatcatgt gcctgatagc aattacatca ccatcagggg tttcgagaac
     4741 agctggcttc tttttgatag ctcctgcagc cataatggca acctgtggct gattgatgat
     4801 gggagtgccc ataacatttc caaacgaacc tacattagtc aacgtaaatg taccaccctg
     4861 aatttcctcc ggtttgagct gattattcct tgctcttgaa gccagatcat tgaccgactt
     4921 ggtaagtccg attaaattca tttcatcggc attcttgatg acagggacga tgaggttacc
     4981 ggaaggcaac gctgccgcca taccgatgtt gatatttttc ttcctgatta tattactgcc
     5041 atctaccgat gcattgatca tgggataatc atgtatcgcc ttgacaattg cttctataaa
     5101 tatcggggtg aatgttatgt tttggttgta tttctgatta aactcctttt ttactttttc
     5161 acgccatcgg accatggacg taacatcgac ttccacaaat gaagtgacat gtggtgacgt
     5221 gtgtttcgac aaaaccatat gatccgcaat caaacgtctc atccggtcca tctcgattat
     5281 ctcaacatct ccatcaaggg aagttgtgga accggaagac ttaggggtac cctgggcttg
     5341 aggctttgat tccacttgcg caacaggagt tgaaggattg acaggttgtt gagaatggac
     5401 ctgtgtagct ggaatagctg ccttgacttt gatataatcg agcaaatcat gcttggtcac
     5461 ccttccatcc tgaccggtgc cttttatcgc atccaattct gtaatactaa taccttcttt
     5521 tgatgctata ttcctgacca acgggctata aaacctcgca ccgtctccca gacttaccgt
     5581 aatcgactgc ttcgtacctt cctccggtac aaaaggtacc ttagaagcag aatgctcttc
     5641 tgaagaggca ctctcatcag taacggcact accgactggt gcccctgttt ctatagttgc
     5701 tatgactgta ccgatgggaa ctacatcatt gacattgaac aatatttttg acaatgttcc
     5761 ttcaacggga gaaggtatct cgctatctac cttgtcggtt gcaatttcaa gaatagtctc
     5821 atcaatatca atatgatccc ctacattctt agcccaattg aggatggttg cctccatgat
     5881 actctctccc attttaggca taaccaattc gtacaaagcc atagttctgt tttaatttcg
     5941 aaaattcatg caaaaataag gttttaacgc ctaatatttt tacatttgaa tccctcatta
     6001 ttacgaatat gtttattctt atatatacaa ttttgggtta taatccatta caatcaaacc
     6061 caccctgttt tctatcataa ataataatgg taggaacctc aagtatatta ttcgatattt
     6121 catttgacat tattttataa gactccaatt agatagggcc ggggcgcaat acagcgattg
     6181 gaagatggag gcttggcaat ttcaattgta atacttgaaa gtgcggatta ttgacattca
     6241 aatgatttag ttggggtgcc catttgaagt gatcgggctt aaaaaatgtt acatgtgcct
     6301 tagaaacggc tttctggcct ttatactcga aaatcgctgt agcggatcta ccgattaaca
     6361 aattcaaact actatcaata ctttaggtat aacatcaatt tcggctgatt aggcctataa
     6421 aactcttaca actggtaaaa gttagacggc caagaaatcg gcagaaaact catcctacaa
     6481 ccaaatatga ataaattact ataaaacagg aataaataaa gagaatttaa acataaggca
     6541 tttcacgggt attgaatcac ccaaagtcct tatatcacag ccatatcggg ctccttagag
     6601 atcctgtgaa tgcattgttc aacgcaattt gaatcctata gtcaacaata ctgcactatt
     6661 ggtcaattta ttattgacca gggtttggct ggcctgggtt atatagtagg gttggtagcc
     6721 attttcagac ctccattgtc tgaagccaag atccagaaag aatacatcac tccgatatcc
     6781 caatccaagg ccaaaatttg gatagaaatt acgctgaccc acaaagggag tttgcgcaaa
     6841 accaacaccg gcacgaatcc tgaagtgctc aatagcagcc acctcaatac caagattgac
     6901 cttaactgta gaggtataat ttgcttttat ctccccattg atctgctctt caacttgctt
     6961 atcatataaa ttatccgaat cgatcctgat cttacttcca gaataatcct gatactccac
     7021 ttcagcattg acaaaaccaa acttcccgat aataccacca atacttccca gtaaccgcat
     7081 cggagtggta actttatagt caatggtccc ttcaggggat tctttataat tgttcttttt
     7141 ttcactattg aaggtaaata cataccttaa atcagtgctg aaattgtctg ttactgagat
     7201 aaagcttggt gtatgtatgg ccgctccgat gagaatatta ttaataggct tgaaaattat
//
LOCUS       DAOQQN010000138         7251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4169_length_7251_cov_4.933574, whole genome shotgun sequence.
ACCESSION   DAOQQN010000138 DAOQQN010000000
VERSION     DAOQQN010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4169_length_7251_cov_4.933574"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1799
                     /locus_tag="PLG24_06495"
     CDS             <1..1799
                     /locus_tag="PLG24_06495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMZ24572.1"
                     /translation="KTFGKVKRKIRTRLKMLEIYLILLIQFVASCTSRPPEPVISASP
                     IKPVITKGYFRSPVDHEIKLAGTFCELRPNHFHTGVDIKSSNGSQGDLIYAIADGFVS
                     RIGISPTGYGYALYIDHPNGFTSVYGHLLHYSSEIEHIAAAEQERQKRFDIQYYPDST
                     ILRIKKGQVIGRMGNSGSSNGAHLHFEIRETETEIPINPLLFGFTSTDKMAPGISYAR
                     IYDLDENFNEFNATNVMAGKRGQIFYPVKGDTITTLSPKIGLGINTRDGQEGNWNKNG
                     VYDITMHVNDTLVYQITMDSVSFDLTRMINAHLDYPEQRLKKNYVHKCYSVPGNILPI
                     IKEQKGHGVIHLQDNEKKKIKFSVKDFNGNMSVMEFYVKKLPGDPVFKYSPLNYFLPY
                     NKKNIIQDTGINIVFTEKTFFKNQNFLFKNEKEKSGRIYSNMYTLGNPNIPVFDYYSC
                     TMDGDQVPEHLKNKAFIASCDEYNRESNWGGTWNENKLTASLRAFGTFYITLDTIPPT
                     VVPVVFGKNMVKNSRIIFRIDDNITPAGQTPYLNYQAFIDDIWIPMVYDLKTKTITYV
                     FPKMFPKGEHSLRLEVFDALKNTKVYTSTFIK"
     gene            1806..2267
                     /gene="bcp"
                     /locus_tag="PLG24_06500"
     CDS             1806..2267
                     /gene="bcp"
                     /locus_tag="PLG24_06500"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HMZ24573.1"
                     /translation="MTRQLQVGDKAPDFKLKDQNGEYMETLAQRGKKYVLFFYPKDNT
                     PTCTKEACNLRDYYSDLKEHGLEIFGISPDSVKSHARFSEMHQLPYSLLADEDHKVAE
                     AYGVWGEKQMYGRKYMGILRTTFVINEEGIIEQAIHKVIAAEHADQILKEC"
     gene            2304..2822
                     /locus_tag="PLG24_06505"
     CDS             2304..2822
                     /locus_tag="PLG24_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HMZ24574.1"
                     /translation="MMSEKLVNALNAQIGMEGYASFLYLSMSGWCEAKGLKGCSAFMR
                     RQSNEEYQHMMKIIDYMLDVDQKPLIPAIAQPASEFNSIVSLMQQVYEHEKKVTRSIH
                     QLVEISNETDDFDTFNFLQWYVTEQREEEALMREILDKINLIGDGPQSLYYIDKELEA
                     INNRQLAAEGKA"
     gene            2848..4227
                     /gene="glmM"
                     /locus_tag="PLG24_06510"
     CDS             2848..4227
                     /gene="glmM"
                     /locus_tag="PLG24_06510"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HMZ24575.1"
                     /translation="MTLIKSISGMRGTIGGHPGENLTPVDIVEMAASFGQMIKKNNSH
                     PSIVIGRDGRISGNMVSQLCIHTLVAQGFIVYDAGLSTTPTIEMAVTKLGADAGIIIT
                     ASHNPIQWNALKFLNEKGEFISAAQGQELIRIAHEKQYLFNEIHNLGKIIPAAGLIEH
                     HIDSILNLPLVNKNIVADKKFKVVVDCINSTGAISVAPLLEALGCEVILINEEMTGNF
                     AHNPEPLAQHLQELCERVISEKAEMGIAVDPDVDRLSFICEDGSLLGEENTLVSVANY
                     ILQHTPGETVSNLSSTRGLKDITLRYGMEYHAATVGEVNVVEKMKEVNAIIGGEGNGG
                     IIYPALHYGRDALVGIALMLTYMAENNLSLTQIKASLPQYFISKNKIDLQPGWNVTAA
                     INGLIDKYSEEEINTSDGLKIDFAKGWVQLRQSNTEPIIRVYSESYDEATANRLAEKI
                     IGEFKELIG"
     gene            4322..5461
                     /locus_tag="PLG24_06515"
     CDS             4322..5461
                     /locus_tag="PLG24_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HMZ24576.1"
                     /translation="MDRIYLDNAATTPLDPEVLDIMCKTLQNDFGNPSSTHSEGRKAR
                     ALIEAARKSIASCIHASPSEIIFTSGGTESNNMALKCAVRDLHVKRIISSRLEHHCVS
                     HTLTYLQRNHGTEVILLDNDEYGRIDLIQLEEYLANSPDKKLVSLMHANNEIGTVADI
                     EAIGNLCRQYDALFHSDTVQTVGHFPLDVSKINIHFLTGTGHKIHGPKGSGFLYIRSG
                     HILEPLIDGGSQEKNLRGGTENLHGIIGLAKSMELAVNHLPERRTYITGIKNYFIEQL
                     RQHIPEVDFNGDLSEDSLYSVLNVSFPAHPKNEMLLLHLDIHGVSVSGGSACSSGADR
                     GSHVIEALDKDPMRKAVRFSFSHHNTFEEVDKVVRMIRSIYYGNA"
     gene            complement(5468..5869)
                     /locus_tag="PLG24_06520"
     CDS             complement(5468..5869)
                     /locus_tag="PLG24_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24577.1"
                     /translation="MKKLILVLCIIIGIGGMFLPWFSLPLIESPSGIELGYSWLGLAL
                     MGINLIPVFLASFDKKSSGIWASLILVSGLLACGYGLLNYFSYKSDILDMGDGNFIAK
                     AVSDTISLGAGYIMYMAAGLVIFLIGLLSKK"
     gene            complement(5925..7130)
                     /gene="sucC"
                     /locus_tag="PLG24_06525"
     CDS             complement(5925..7130)
                     /gene="sucC"
                     /locus_tag="PLG24_06525"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HMZ24578.1"
                     /translation="MNLHEYQGKELLAGYGVAVQRGFVAENVDQAVEAFKRVNEELGS
                     QFAVVKAQIHAGGRGKGGGVKLVKNLDELKAEAGRIIGMYLRTPQTPGGMEGPGKLVR
                     KVFIAEDSYAPDFAACKEFYVSVLLDRGTGKNVIVYSTQGGMDIEKVAEETPELVLKE
                     MVDPTLGLLPFQARKIAFNLGLSGKAFKEFVVFIDRLYKAYVGSDASLFEINPLIKTG
                     DDRLVAVDAKVSIDDNALYRHKDLEEAYDKLEEDPADLEAKDADLNYVKLDGNVGCMV
                     NGAGLAMATMDMIKLIGGSPANFLDVGGTADAARVEKALNLILKDPSVKAILINIFGG
                     IVRCDRVAGGVIEAYKNIGNIPVPIIVRLQGTNAEIAKKMIDESGLKVLSAIELKEAA
                     EQVSKALAG"
BASE COUNT         2298 a         1555 c         1474 g         1924 t
ORIGIN      
        1 gaaaaacatt tggaaaagtt aaaagaaaga ttcggactag attgaaaatg ctggaaatat
       61 acctgatact gttgattcaa tttgtagcct cttgtactag cagaccacct gaacctgtga
      121 tctctgccag ccctattaaa ccagtaataa caaaaggata tttcagaagt cctgtcgatc
      181 acgaaattaa gctggcgggc accttctgtg agttaaggcc aaatcatttc cacacaggtg
      241 tagatatcaa gtcctcaaac ggcagccagg gtgatcttat ctatgccata gcagatggct
      301 ttgtgtcacg gataggcatc agccctaccg gatatggcta tgctctatat atcgaccatc
      361 caaatgggtt taccagtgta tatggacacc tccttcatta cagcagtgaa attgagcata
      421 ttgcggctgc agaacaggaa aggcaaaaac gttttgacat ccaatattac ccggacagta
      481 cgatattgag aataaaaaaa gggcaggtca taggtaggat gggtaattca ggttcatcca
      541 acggcgcaca tcttcatttc gaaataagag aaactgaaac ggaaattccc atcaatccat
      601 tattgtttgg ttttacctca accgacaaga tggctcccgg aatcagctat gccaggatat
      661 acgatctgga tgaaaatttc aacgagttca atgcaacgaa cgtaatggcc gggaagcgag
      721 gacaaatatt ttacccggta aaaggagata ccataactac cctttccccg aaaattggcc
      781 tgggtatcaa taccagagat ggtcaggaag gcaactggaa caagaatggg gtatatgaca
      841 ttacaatgca tgtcaatgac accctagtct atcaaataac aatggattct gtatcatttg
      901 atttaacccg gatgatcaat gctcatctgg actatccgga acaaaggctc aaaaaaaatt
      961 atgtacataa atgttactct gtcccgggta atatactgcc aatcatcaaa gagcaaaaag
     1021 gtcatggtgt aattcatctt caggataatg aaaagaaaaa aataaaattt tctgttaagg
     1081 attttaacgg gaatatgtca gtaatggaat tttatgtcaa aaaactcccc ggtgatccgg
     1141 tatttaaata ttcaccctta aattattttc ttccttacaa caagaaaaat attatacagg
     1201 ataccggtat caacatagtt ttcacagaaa aaacattctt taaaaatcaa aatttcctgt
     1261 tcaaaaatga aaaagaaaaa tccggacgga tttattcaaa catgtacacg ctggggaacc
     1321 caaatattcc ggtctttgat tattactcat gcacaatgga tggagatcag gtaccggaac
     1381 atttgaagaa caaggcattt attgcctcct gtgatgaata taaccgagag tccaactggg
     1441 gcggaacatg gaatgaaaat aaactaactg cttcattgag ggcatttggt acattctata
     1501 taacgttgga tactatcccc cctactgttg tacctgttgt tttcgggaaa aatatggtta
     1561 aaaactcccg catcatattt cggatcgatg ataacattac cccggcaggt caaacaccat
     1621 atctcaatta tcaggcattt atcgacgata tctggattcc tatggtatat gatctgaaaa
     1681 caaaaacaat tacttatgtg tttccaaaaa tgtttcccaa gggtgaacat agtctaaggc
     1741 ttgaagtatt cgatgccctg aaaaacacga aggtgtacac ttctacattt attaaataat
     1801 tgaaaatgac cagacaattg caagtaggtg acaaagctcc ggatttcaaa ctgaaggatc
     1861 aaaatggaga atatatggaa accttggccc agagagggaa gaaatatgtt ttattttttt
     1921 atccaaagga caatacaccg acttgtacta aagaagcctg caatctaaga gactattata
     1981 gcgatcttaa agaacatgga ttggaaatct tcggtattag tccggattca gtaaaaagtc
     2041 atgcccgttt cagtgagatg catcagttgc cctattcact cctggctgat gaagatcaca
     2101 aggttgcaga agcgtatggt gtatgggggg aaaaacaaat gtatggaaga aaatatatgg
     2161 gcattctgcg aacgactttt gtcatcaatg aagaaggaat catcgaacag gcaatacaca
     2221 aagtaattgc tgcggaacat gccgatcaaa ttttaaaaga atgttaaaaa cctcattttg
     2281 aaaaataaaa tattcagtta tctatgatgt ccgaaaagtt agttaatgcg ttgaatgcgc
     2341 agattgggat ggaaggctat gcctcctttt tatacctgtc catgtcagga tggtgcgaag
     2401 ccaaaggcct aaagggatgt agtgcattca tgcgacgcca gtcaaatgaa gaatatcagc
     2461 atatgatgaa gatcatcgat tatatgctgg atgtggatca aaaaccgttg attcccgcta
     2521 ttgctcagcc agcctcagag ttcaattcca tcgtttcgtt gatgcaacag gtatatgaac
     2581 atgaaaaaaa agtaacccgt tccatacatc agctcgtgga aattagtaat gaaacggatg
     2641 atttcgatac attcaacttc ctgcaatggt atgtaacgga acaaagggaa gaggaagctc
     2701 ttatgcgaga gatattggac aagatcaacc tgattgggga tggcccacaa tctctttatt
     2761 acatagacaa ggaattggag gccatcaata accgtcagtt ggctgcggaa ggaaaggcct
     2821 gattgtttta ctcttaagaa caaacttttg actctaatca aatctatatc agggatgcgt
     2881 ggtacgatag gcgggcatcc cggagaaaac ctcaccccgg ttgatatcgt ggagatggcc
     2941 gcatcgtttg gccaaatgat caaaaagaac aattcacacc cttcaatagt aattggcagg
     3001 gatggcagga tatccggcaa tatggtatcc caactatgca tacacacctt agtggcgcaa
     3061 ggttttattg tatatgatgc aggactttcc accacgccga cgatagaaat ggcagttaca
     3121 aagctgggag ctgatgccgg aattattatc acagcaagcc acaatcccat tcagtggaat
     3181 gctttgaaat tcttgaacga aaagggagaa ttcatatcag ccgctcaagg tcaggaattg
     3241 atccggattg ctcatgaaaa acagtatcta tttaacgaaa ttcacaatct aggtaagatc
     3301 attcctgctg caggcttgat agagcatcat attgattcca ttctgaatct tccattggtc
     3361 aataaaaata tagttgcaga caaaaaattt aaagtcgtgg tcgattgtat caattcgacc
     3421 ggagcgattt cagttgcacc actgctggag gctttgggtt gcgaggtcat cctaattaat
     3481 gaagagatga caggcaactt tgcccacaat ccagagcctt tggcacaaca cctgcaggaa
     3541 ttgtgtgaga gggtgatatc agaaaaagcc gaaatgggta tcgccgtgga tcctgatgtg
     3601 gatagattaa gctttatatg tgaagatggc agccttttgg gagaagaaaa tactttggta
     3661 tcagttgcaa actacatact ccaacatacg cccggtgaaa cggtttccaa cctttcgtca
     3721 accagagggc tgaaggatat tacccttaga tatggaatgg aatatcatgc tgctactgtc
     3781 ggagaggtta atgtggtaga gaaaatgaag gaagttaatg ccattatcgg tggagaagga
     3841 aatggcggca tcatttatcc tgccctgcac tatgggcgtg atgcactcgt gggaatagcg
     3901 ctcatgctca cctatatggc ggaaaataat ctcagtttaa ctcagataaa agcttctttg
     3961 ccccaatatt ttatttcaaa aaataaaatt gatctgcaac cgggctggaa tgtcacggca
     4021 gcaatcaatg gcttgattga caaatattcg gaggaagaaa tcaatacctc agatggcttg
     4081 aaaatagact ttgccaaagg ctgggttcaa cttcggcagt ctaatactga gcctattatt
     4141 cgggtttata gcgaatctta tgatgaagct accgcaaata gactggcaga gaaaatcatt
     4201 ggtgagttca aggaattgat tggatgacat ccgggatcct caaaaacatc attgatccta
     4261 acttcatgtt acttaaccgg ttataaaaat gacaatcttt catttcaaac aattgtaatc
     4321 catggacaga atatatcttg acaacgcagc caccacccca ctcgaccctg aggtgctcga
     4381 cataatgtgt aagacattgc aaaacgactt cggcaatccc tcaagtacac attcagaggg
     4441 ccgtaaggca agggccttga tcgaggcggc ccgcaaatct atcgcttcct gtatccatgc
     4501 ttctccaagt gaaatcatct ttacctcagg cggtactgaa tccaacaata tggctttgaa
     4561 gtgtgcagtt cgagaccttc atgtcaaaag aatcatatct tccagattgg agcatcattg
     4621 cgtcagccat acactcactt atctgcaaag gaatcatggc acagaagtca tcttactcga
     4681 caacgacgag tatggcagaa tagacttgat ccaactggaa gaatatctgg ccaatagccc
     4741 ggataaaaaa ctggtctccc ttatgcacgc caacaatgaa attggtacag tcgcagatat
     4801 tgaagccatt ggcaatcttt gccgtcagta tgatgcacta tttcattcgg atacagtgca
     4861 aacagtaggt catttccctt tagacgtgtc taagattaat atccatttcc tgacaggaac
     4921 aggtcacaaa atccacggcc cgaaaggcag cggatttctg tatatccgtt ccggccacat
     4981 tctcgaacca ttgattgatg gtggttcgca agagaaaaat ctccggggag gcactgaaaa
     5041 cctccatggc atcataggtc tggcaaaatc tatggaactt gctgtcaacc atttgccgga
     5101 aagacgtacc tacattacag gcatcaagaa ttattttatc gaacagttaa gacaacatat
     5161 tccggaagtg gatttcaatg gtgacctcag tgaagattcg ctttatagcg tactgaatgt
     5221 ttctttcccg gcgcatccca aaaacgaaat gttgcttttg catctcgaca ttcatggcgt
     5281 ctctgtctcc ggaggaagtg catgctcttc aggtgctgac cgaggtagcc acgtcatcga
     5341 ggctttagac aaggatccaa tgaggaaggc tgtgcgattc tcgttctctc accacaatac
     5401 atttgaagag gtggacaagg tcgttaggat gatcagatcc atttattatg gaaatgctta
     5461 aggcgactta tttcttcgaa agtaatccta tcaggaaaat aaccagtcct gcagccatat
     5521 acataatata accggcacct aaggaaatgg tgtcagatac tgcctttgca atgaaattgc
     5581 catcacccat gtctaaaata tctgacttat aactgaaata attcaacagt ccataaccgc
     5641 aggctagcag gccggatacc aaaattaagg aagcccaaat tccggaagat tttttatcaa
     5701 atgacgctaa aaataccggt attaaattga tacccatcaa tgccaatcca agccaggaat
     5761 atcccagttc gattcctgaa gggctttcaa tcagtggcaa agaaaaccag ggcaagaaca
     5821 tacctcctat accaatgatg atacataaaa ccagaattaa cttcttcaat tcaattcaaa
     5881 aatttgaagg aaaaaaaagc cggaaataga tccggccttt atttttaacc tgccagagct
     5941 ttagatacct gttccgcagc ttcttttaat tcgatggcag aaagtacctt tagccctgat
     6001 tcgtcgatca ttttcttagc aatctcggca ttggtgccct gaagccggac gatgattggt
     6061 acaggaatat tcccaatatt tttataggct tctataacgc ctccggcaac acggtcgcag
     6121 cgcacgatcc ctccgaaaat attgatcagg atggccttta cggaaggatc cttaagaatg
     6181 agattaagcg ctttttccac cctcgcggca tctgcagtac ctccaacatc caggaagttg
     6241 gccggagagc ctcctatcaa cttgatcata tccatagtag ccatggccag acctgctcca
     6301 ttgaccatac agccaacgtt tccatccagc ttgacgtaat tgagatcggc gtccttagcc
     6361 tcaaggtctg ccggatcttc ctccagctta tcataagcct cctccagatc tttatgtctg
     6421 tacagagcat tatcgtcaat ggagaccttt gcatcgacag caaccaaacg atcatctcct
     6481 gttttaatca gcggattaat ttcaaacaaa cttgcatcgg agcctacata agccttatat
     6541 aagcgatcaa tgaatacaac gaattctttg aaagccttac ctgacaatcc aagattaaag
     6601 gcaatctttc ttgcctgaaa tggaagaagt cccaacgtag gatctaccat ctccttcaaa
     6661 accaactccg gagtttcttc tgccactttt tctatatcca ttcccccttg tgttgaataa
     6721 acgatcacat tctttccggt ccctctgtca agtaaaacag aaacataaaa ttccttacat
     6781 gcagcaaaat ccggtgcata ggagtcctct gctataaaaa cttttctaac cagttttccg
     6841 ggaccttcca tgcctccggg agtctgaggt gtacgcagat acataccgat gatacggccg
     6901 gcttctgcct tcaactcatc cagatttttg accagcttta caccaccacc ctttcctcgg
     6961 ccaccggcat gaatctgagc cttcaccaca gcaaactgtg aacccaactc ttcattgacc
     7021 cttttaaatg cttcgacagc ctgatcgaca ttctctgcta caaatcctcg ctgaacagcc
     7081 acgccgtatc cggcaagcaa ctcttttccc tgatattcgt gtaaattcat tgtatattgt
     7141 ttataactga tttaatataa tgctgcaatg atacaaaagt atattcagat aaaaggaacc
     7201 caaatgtcat taaataacag tgccccaaaa attatcccgg ggcattatca t
//
LOCUS       DAOQQN010000139         7245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4176_length_7245_cov_5.893046, whole genome shotgun sequence.
ACCESSION   DAOQQN010000139 DAOQQN010000000
VERSION     DAOQQN010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7245
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4176_length_7245_cov_5.893046"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="PLG24_06530"
     CDS             complement(<1..1049)
                     /locus_tag="PLG24_06530"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMZ24579.1"
                     /translation="MTKIEEMAIRLLTLLTIGGLFTSCGSKKSIDLLVYNATIYTVDS
                     TFSIAEAFAVDKGKIVETGRSEDLKSKYNARESLDAGGKYVYPGLIDAHAHFIGYGQS
                     LNRVSLVGTTSWEDCVNRTREFAKKNDVEWIQGRGWDQNDWAEKEFPDNQALNELFPD
                     IPVFLTRIDGHAAIANDKALQITGIKAGDKIDGGIIEVKDGKLTGVLVDNAVDLVSSK
                     IPPMNVSELTEALLEAQLNCFAVGLTTIDDCGLDHEVIDVIDSLQKSGQLKMRLYVML
                     SDVRKNYKYLIDKGKIKTDKLNVRGFKIYSDGALGSRGACLLHPYSDKPGWTGFLLSS
                     KEHFDSVANVIYTHG"
     gene            complement(1538..2530)
                     /locus_tag="PLG24_06535"
     CDS             complement(1538..2530)
                     /locus_tag="PLG24_06535"
                     /inference="COORDINATES: protein motif:HMM:NF024599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCMD domain-containing protein"
                     /protein_id="HMZ24580.1"
                     /translation="MNTGSNSIRKLLRYSCVIVFLLAACHAAFSQTRFIPFAFGDMDK
                     WINRDVKESFVIGGKTQTLYEIADTRDGVSPNHAYDNKFSPWATSSVLATINGITKGS
                     VTVFPEKRGDGFAARLETRVERVKVLGILNINVLASGTIFLGKVAEPITDTDNPFSKL
                     IMGIPFTGKPAWLQFDYKVTAGGSSRKINGLSSNGTATGKADNAIVHVYLQKRWEDKD
                     GNVYALRIGTGGEIFSQTINDWQKSHRVKIHYGNVSNYGNLPAIFTRLKKGNDAFYTR
                     NSKGKMVPIHEEGWGSPNNEVTHIILQFSSSDGGPYVGSPQNKLWVDNVGTVYD"
     gene            complement(2789..3226)
                     /locus_tag="PLG24_06540"
     CDS             complement(2789..3226)
                     /locus_tag="PLG24_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24581.1"
                     /translation="MLRLRCVEPEDEENTRTELPEKLSTFEELEVNGEAESFQKAFDV
                     ITGKRPDSAFMDIKLIGGSIFILLEKLISHGIPIPNIFIATGFSENLLTTDNAFHNYI
                     VRHLIKPYMDDWEVKLSKAIDALQTTRIRQLITTEGNKLPESV"
     gene            complement(3341..3733)
                     /locus_tag="PLG24_06545"
     CDS             complement(3341..3733)
                     /locus_tag="PLG24_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24582.1"
                     /translation="MARKATVTLNVIDSLAKKIKSLLNNTLQKVSFHNLRKEMYLSSS
                     YLFILFIAFEKSFINYEGKDSGRTKAPSFSSKATQFGAVRLRELENKYNQNNLLLLLQ
                     TTRVEIIADKSGSHHITRIVTRILKKIN"
     gene            4558..7023
                     /locus_tag="PLG24_06550"
     CDS             4558..7023
                     /locus_tag="PLG24_06550"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HMZ24583.1"
                     /translation="MLDLTARKICEIVEGTLYGLSERQLIRRISFDTRQIFHAHEVMF
                     IALPGEHRDGHNYIKKAWDAGVRCFLVSRKVDFESFGDSVFILTENTLLGLQAIAAYH
                     RSLFKIPVIGITGSNGKTIVKEWLYQMLSGEINIAKSPKSYNSQLGVPMSVITLRPEN
                     ELAIFEAGISQKGEMDRLEAIIQPTIGILTYMGAAHSEGFESKTAKIEEKLKLFKHAD
                     FVIYQKKDNTHEAIQRVLPETRKFSWGVNVDADVIFIIEKVKTRKTIASVKYLGNIYK
                     IKIPFTDDIYLHNAFTCITYLIHAGYSIELIESMIGKLQPLPMRMELKTGIFNSVLIN
                     DTYSSDINSIALAFAAARKEAGKGRMAFIVSDFAYSDNHTHDDYEVLVSLVNEFRPAL
                     FIGVGEKVRNIRIKMDPEIRASFFHNTESLLQGMDITQFRDTTVLLKGARRFGFEQIV
                     DRLEQRAHEAALEINLSALEHNLNRYRAKITYPVKLMVMIKASAYGSGSVEVARVLQQ
                     QGIDYLAVAYIDEGIYLRKAGISLPIMVMNSGMDFVERMVEYDLEPEIYAPAQLDVLI
                     QYLFSADERLKCHIKLDTGMHRLGFTKDEIPFLIEKLKGNSHLEVVSVFSHLAGSGES
                     LFDEYTHFQAAYFEEMVSSISRGLRTHPLRHILNSSGIARFPQYMYEMVRLGIGLYGF
                     DPSGFVSDLHTVMTLKARISRIKTIPTGSTVGYDRRWKAEKDSIIATISIGYADGLRR
                     SAGNGRWKVLIHGQPATIIGGVCMDMAMVDATHIEGVREGDEVVIFGPTNRADELAKV
                     YETIPYEVFTGISSRVKRTYILE"
BASE COUNT         2139 a         1350 c         1506 g         2250 t
ORIGIN      
        1 aagccatggg tatatatgac attggcaact gagtcaaaat gctctttaga actcaacaga
       61 aaaccggtcc atccgggctt gtccgaatat gggtgtagca gacaggcgcc acgggaaccc
      121 aaagcaccgt cagaatagat tttgaagccg cggacattta gcttatccgt cttgatcttt
      181 cctttgtcga tcaggtattt gtagttttta cggacatcac tcaacatcac ataaagccgc
      241 atctttagtt gacctgactt ttggagtgag tctatcacat caataacctc atgatccaga
      301 ccacagtcat cgatggtagt caagcctaca gcaaaacaat ttaactgtgc ttcaagtaat
      361 gcctcagtca attcggatac attcataggt ggaattttgg agctgaccaa atcaactgca
      421 ttgtccacca ggacaccggt caattttccg tcctttactt cgatgatgcc gccatcgatt
      481 ttatcgcctg ctttaatgcc tgtgatttgt aatgccttat cattggctat ggccgcatgg
      541 ccgtctatcc tggtcagaaa aaccggtatg tctggaaaaa gttcgtttag tgcctggtta
      601 tcgggaaatt ccttttccgc ccaatcattc tggtcccagc cacgcccctg gatccattcc
      661 acgtcatttt ttttggcgaa ttccctggtt ctgttgacac agtcctccca acttgttgta
      721 cccaccaggc taaccctatt aaggctttgt ccgtagccta tgaaatgggc atgtgcatcg
      781 atcaggccgg gatagacata ttttccgcct gcatccaaac tctcccgtgc attgtatttg
      841 cttttcagat cttctgacct gccggtttcc actattttgc ccttgtccac agcaaatgcc
      901 tctgcaatgc tgaatgtgct gtcaactgta tatattgtcg cattatagac tagcaggtcg
      961 atggactttt tacttccaca tgacgtaaac agaccgccaa tggtcaaaag agtcaacaat
     1021 ctaatagcca tttcctcaat tttagtcatt caaattagtt tttgaagaga atacattacc
     1081 ttgacttttt cattttcgtt ggtgctacaa atgtaatcag ccttaaccaa agaatcgaaa
     1141 gagactgcca aaaaatcaaa actagcctta attcatgatg tggcagcaac acagagagtt
     1201 tgttcctggc taatactcgt gtattataat gatactattt gtaatacaaa agtaagtgtg
     1261 acgaccgaca aacattgtat atcatggata gatctatata gaataatgat gatttcaaat
     1321 gaaggaagag tacatataga tcattattgg gtagaaagct gatacgatta attggagttc
     1381 ctgcaaaaaa attgattaca tatttgggca aaattcaccc ggaacccaaa ggttattcaa
     1441 ttgctgtacg gggtcaggat acatggtaat tcaaaatcaa atctccggtc tcccatctca
     1501 aaatatgtaa tatggaaata aatttccaac atacatttca atcatacact gttccgacat
     1561 tgtccaccca tagcttgttt tgagggctac cgacataggg tccgccatca cttgatgaaa
     1621 actgcaagat gatgtgagtg acttcattgt tggggctacc ccagccttct tcatggatgg
     1681 gaaccatctt ccctttactg tttcgggtat agaaggcgtc atttcccttt ttgagacggg
     1741 taaaaatggc aggcagattg ccatagttgg agacattgcc gtaatgtatc ttgacacggt
     1801 gacttttctg ccagtcattg attgtctgag agaaaatctc gccgccagtg cctatccgga
     1861 gagcatatac attgccatcc ttatcctccc atcgtttctg gagatagaca tgtactattg
     1921 cattgtcggc cttgccggta gcagtaccat tgctgcttaa gccattgatc ttacgtgatg
     1981 atcctccggc cgttacttta tagtcaaatt gtagccaagc gggctttccc gtaaatggga
     2041 tacccataat aagcttactg aatggattgt ctgtatcagt tatgggttct gcaaccttgc
     2101 ccagaaatat ggtgccggag gcaagaacat tgatgttgag tatacctagc accttaacac
     2161 gttctacgcg tgtctccaga cgtgccgcaa agccatcacc ccgcttttcc ggaaagacag
     2221 tcacactgcc cttggtgatg ccgttgatgg tagcaagtac agaagaggtt gcccatggag
     2281 aaaacttgtt gtcgtaggca tgattaggtg agacaccgtc tcgagtgtca gcgatttcgt
     2341 aaagtgtctg tgtcttacca ccaatgacga atgattcctt tacatcacgg ttaatccatt
     2401 tgtccatatc cccaaatgca aaggggatga acctggtctg actaaaggct gcgtggcagg
     2461 ctgctaataa aaatactatg acacagctat atctaagcag cttccgaatg gaattagaac
     2521 ctgtattcaa gagtctatct ttgaaattga ataatttttt gagcaattgt atgatgattt
     2581 taaaatgtaa cgatgaaata accgagatta tgataaaaga ggacagataa aaatttacct
     2641 cattctaaat cttattgcct ggattaattg ttattgcaaa tgttatgata gaatatgatt
     2701 gtgatcttgc cttttacatc gtttttagat atcatacatc aagatattgt tttctgatta
     2761 ggttcttccc ggattgtatg gatgaatatt aaactgattc cggaagtttg ttcccctctg
     2821 tcgttatgag ttgtctgatt cgggtagttt gcagggcatc aatggccttg ctaagtttga
     2881 cctcccaatc atccatgtaa ggtttgatga ggtgccggac aatgtagttg tgaaatgcat
     2941 tgtccgtagt caataggttt tctgaaaatc ctgtggcaat aaaaatattg ggaattggga
     3001 tgccatggga tattagtttt tcaagaagta tgaaaatgct acctccgatc aatttgatat
     3061 ccataaatgc cgaatcgggc ctttttcctg ttatgacatc aaaggccttc tggaaacttt
     3121 ccgcttcgcc gtttacttcc agctcttcaa acgtggacaa cttctccggc aactcagtcc
     3181 gcgtattttc ttcatcttcc ggttctacac atcttaatct caacaagtta gtcgtaaact
     3241 aaattttgga tttatagata tgttgacaat tttttactat agggttatac taagggtctt
     3301 taaaaattat ccataggcgt tatttttttt caggtaatta ttaatttatt ttttttagaa
     3361 ttctcgtcac aatcctcgtt atatggtgac ttcctgattt atctgcaata atttcaacac
     3421 gagttgtttg caataaaaga agtagattgt tttgattgta tttattttca agttctctga
     3481 gcctgactgc tccaaattga gttgcttttg aactaaaaga aggtgcttta gtgcgtccag
     3541 aatctttgcc ttcgtaattg atgaagcttt tctcaaacgc gataaacaga atgaataaat
     3601 aactggagga tagatacatc tctttcctta ggttgtgaaa actaactttt tgaagggtat
     3661 tgttcaatag ggatttgatt tttttagcaa ggctgtcaat cacatttaaa gtcacagtgg
     3721 cttttcttgc catgaccgga agctttagca aggtattgtg gatgaaataa ggattgggcc
     3781 aattgaaagg tgcataaacc ctatggacgt ccatatccct atacatcatt ggaaaatttg
     3841 tcttttgatg ggatatcatg aatttatgct tataaatttt tatttacttg tcacacaaaa
     3901 ataggcttac atattcagta gtaaaaagaa tgacccgcca accgtgtaat ttgacccaca
     3961 aaaggggtga aatgacgaac gaaagccttt ttgcctacaa aataatccca ttcttctttg
     4021 aacattgtct tacataatat cttctgcaat tcaagtttag gtacatcacg tgatcttcat
     4081 acatctaagg catccaggtc cataattcag aggatattat attgacagat tgtaatagag
     4141 taaactcgct gagatggtat tgaaatggaa cataatgtat ggttcacatt acaggataat
     4201 tattctttca aaatttataa aatctatatc aaatgaaatt attcagtctt ttcttcctca
     4261 ctcaagaatg ggttctaaat ggtttcatcg ctcagtagta tgcaaggttt tattttcact
     4321 ctgaaaggta atagcttgaa atagtgacta attatccgag caaactttct taatcagaca
     4381 gaaaaaatga aagaaaagat aatgaatcat gtgaagattg aaaaggataa tacatagaga
     4441 tcagtaattg gaagaatata gtagacgaat tataactacc aatactaaat ttggattcaa
     4501 tgcttataaa tatatcaaaa atatatagtt tagtaataac tttgtattgt tgcaaatatg
     4561 ctagatctaa ccgccaggaa aatttgcgaa attgtagagg ggacactcta tggtttgtct
     4621 gaaagacagt taattcgtcg gatatcattt gatacaaggc aaatttttca tgcccatgaa
     4681 gtaatgttta tcgccttacc aggcgaacat cgtgacgggc ataattacat aaaaaaagca
     4741 tgggacgccg gcgttcgatg ttttttagtg agcagaaaag ttgatttcga atcattcggt
     4801 gactcggttt ttattttgac cgaaaatacc ttgttgggat tgcaggcaat tgccgcttat
     4861 cacaggagtc ttttcaaaat ccctgttata ggcatcacag gaagtaatgg aaaaaccatc
     4921 gtcaaggaat ggctttatca gatgttatcc ggtgagatta acattgcaaa aagcccgaaa
     4981 agttataata gccaactcgg agtgcctatg tcagtcatca cgctccgtcc ggaaaacgaa
     5041 cttgccattt ttgaagcagg tatttctcaa aaaggagaga tggataggct ggaagccatc
     5101 attcaaccaa caattggtat tctgacttat atgggagcag ctcacagtga gggttttgag
     5161 tcgaagacgg ccaagataga agagaaatta aaacttttca aacatgccga ttttgttatt
     5221 tatcaaaaaa aagataacac acatgaagct attcaaagag tacttccgga aacgaggaaa
     5281 ttcagttggg gggtaaatgt agatgcggat gttattttca tcatagaaaa agttaagaca
     5341 cgcaagacaa ttgcatctgt aaaatatttg ggcaatattt ataagattaa gattccattt
     5401 actgatgata tatacttaca taatgccttt acatgtatta catatctgat tcatgccgga
     5461 tatagtattg agttgattga atccatgata ggtaaattgc agccactacc gatgagaatg
     5521 gagcttaaga caggtatatt taattcggta ctaatcaatg atacctatag ttcagacatc
     5581 aattccatag cattggcatt tgctgcagcc agaaaagaag ccggcaaagg aaggatggct
     5641 tttattgtct ctgattttgc atattcggac aatcatacac atgacgatta tgaagtgttg
     5701 gtgagtcttg tgaatgaatt tcgcccggca ctgtttatcg gggttggaga aaaggttagg
     5761 aatatcagga tcaaaatgga tcctgagata cgcgcttcat tttttcacaa cactgaaagt
     5821 ttgttgcaag gtatggatat cacacagttt cgggacacta ccgtgttact gaaaggtgcg
     5881 cgtcgtttcg gttttgaaca aattgttgac aggcttgaac aaagggctca tgaagctgct
     5941 ttggaaatca atctttcggc tttggagcac aatctaaatc gctaccgagc taaaataacc
     6001 taccctgtca aactcatggt gatgattaag gcaagtgcat atggttcggg ttctgtggag
     6061 gtggcccgtg ttcttcagca acaaggcatt gattatcttg cggtagccta tattgacgaa
     6121 ggaatatatt tgaggaaagc cggaatctcc ttgccaatca tggtaatgaa tagtgggatg
     6181 gattttgtcg agcgaatggt tgaatatgat ctggagcctg aaatatatgc acctgcacaa
     6241 ttggatgtat tgattcaata cctttttagt gctgatgaac gattaaagtg tcatattaaa
     6301 ctggataccg gtatgcatcg gcttggattt acaaaagatg aaataccttt tttaatagag
     6361 aagttgaagg gcaattcaca tttggaggtg gttagcgtat tttctcatct tgccggtagt
     6421 ggagaaagcc tttttgatga atacacgcac tttcaggcag catattttga agaaatggtc
     6481 agttctattt ccaggggttt gagaacccat cctttgcgac atattttaaa ttccagtgga
     6541 atcgctcgtt ttccacaata catgtatgaa atggttcgcc tggggattgg gttatacgga
     6601 tttgatccaa gtggctttgt ttcagacctg cataccgtta tgaccctcaa ggcgagaata
     6661 tcgcgtataa agacaatacc gacaggcagt accgttgggt acgatcgacg ttggaaggca
     6721 gagaaggatt caatcatcgc aactatttca attgggtatg ccgatggttt gcgtcgttca
     6781 gcgggtaatg gcagatggaa ggtgcttatt catgggcaac ccgccaccat tattggaggg
     6841 gtgtgtatgg atatggctat ggttgatgca actcatatag aaggggtaag agagggtgac
     6901 gaagtcgtaa tttttggccc tactaaccga gctgatgagt tggcaaaagt gtatgaaaca
     6961 ataccttatg aggtatttac aggaataagt tcaagagtaa aaagaactta tattttggaa
     7021 tgattataat tatcattact tttattctgc tgaaaatcag tacgtacttg tatcgtgaag
     7081 catttccctt gtaagatttc aattagaagg taattgctca gtgaagttag aatcgaattg
     7141 gtagaggttc agcaattaat caattcagat agattttacc ttttttactt tcatcggcct
     7201 atgcctgacc attctggaga tgatgtcatc caggatttca ccttt
//
LOCUS       DAOQQN010000140         7143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4276_length_7143_cov_5.181433, whole genome shotgun sequence.
ACCESSION   DAOQQN010000140 DAOQQN010000000
VERSION     DAOQQN010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4276_length_7143_cov_5.181433"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PLG24_06555"
     CDS             complement(<1..279)
                     /locus_tag="PLG24_06555"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HMZ24584.1"
                     /translation="MKILMVCLGNICRSPLAEGIMRAKAESAGLKIKVDSAGTSDYHI
                     GEAPHRNSVRIASEHNIDITGLKARQFTKADLETFDLIYVMDESNYKNV"
     gene            complement(362..793)
                     /gene="ybeY"
                     /locus_tag="PLG24_06560"
     CDS             complement(362..793)
                     /gene="ybeY"
                     /locus_tag="PLG24_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HMZ24585.1"
                     /translation="MPTTKIHFFSEGEHPPIRFSKAKFRAVAGKVFADHHKIVGYVNI
                     IFCNDEYLLEINRSYLDHDYYTDIITFDYDDDRVESDIYISTERVLENAGKADDGGTR
                     ELYRVMLHGCIHLCGFGDKTEKEVAVMRQKENYYLEMVNSD"
     gene            complement(793..1200)
                     /locus_tag="PLG24_06565"
     CDS             complement(793..1200)
                     /locus_tag="PLG24_06565"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ24586.1"
                     /translation="MKQVNVIRISSRLKSVSEVETFAKTLQRELNLDEELFPKILISV
                     TEAVNNAIIHGNQIDENKEVTLQSYCKEDKLTICIKDEGPGFEVAKVPDPCCKENIEK
                     PGGRGVLIMRSLSDDIRFKNNGSCVEMTFNIKK"
     gene            complement(1197..4538)
                     /locus_tag="PLG24_06570"
     CDS             complement(1197..4538)
                     /locus_tag="PLG24_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 domain-containing protein"
                     /protein_id="HMZ24587.1"
                     /translation="MESGKQYNLLIQKLDQFIRKFYLNRIIRGSLYLLGFVTVYFMAI
                     ALLENFFYFGTAGRKFLFYSFLLASLFTLIRWIIVPAMKFFRLGSTISNEQAAAIIGD
                     HFGNVKDKLLNVLQLNEQARNNPDSSLLIAGIEQKTKELQPIPFKNAIDLSSNKKYLK
                     YSVPPVLALLLMLFTAPSLIKKPVNRLINNDKEFEREAPFTFQILNKNLKVAEFDNYT
                     LEVKLEGNSIPDQIDVVYHDNPYRLVKDSRGIFTYTFANVQDAFDFRLKAAGFNSKVY
                     RLDVMKKAGMTDLNLKLVYPGYIGRPEEYVKGSGDIIVPFGTKVYWDIRTRLADRVEL
                     EFEGNHAVSAQRAGDNRFTFLKTAIQDQNYKIFTGNQALPVSDSMTYSIHVVMDENPV
                     ISLDQYVDSANQKLVYLSGNVSDDYGFTKLQMVQQIKSEDGKVRTRTLPLQKPATKQG
                     FYSYLFDIESLKLKPGDELTYFFEVFDNDGAKGPKSARTSVMNFKKPSVKEMEKNIDK
                     STREIQSDLEKMVKESKKVNDAINKLREKLLQQKSLNWQDKEQLNKLLEKQKELQDQL
                     KETQKKLEEKRKAEEEIKQPDPEMKEKQEKLDEMIKEMQNPEEQKLMDKIRELMDKLD
                     KKESLKQLEQMSQQNEQQHKSLDRLMELMKQLELEAKVQDQIDKLNELAEKEEKLAEQ
                     TENNQKDQEQLKKEQDQINKDFQEVKKKMDEIQKENKELKAPKKLNDQKEEQQSTEQD
                     LQDAKEQLEKKENKSASKSQKKAGQKMKQMAQNMQNDMAASEKEQIELDMKAVRQLLE
                     NIVGLSFNQEDLIKQYNATMINTPRYIGLNREQKKIKSDFKMVDDSLQELSKRVMQIQ
                     SFIIKKVAEVNDNLDKTMDNLEDRRKPEAAVNQQQSMTSLNDLALMLSEALQQMQMQA
                     QSMPGNASCSKPGGSNPQDGKTGKPGEMPQMDKISQGQQKMTEGMKQQMEQMKNGKKP
                     GSEEFGQLAAQQAKIRKMLQDRDAQMRQNGQGKKQIQDIIDAMDQIEKDLVNRRLTNE
                     MLRRQQDIMTRLLEEERAEREQDKDEQRKATQAQQQQNKIPSSMQEYIKKREAGIDDI
                     RPVYPALTPYYKRLVEQYYNQLSGR"
     gene            complement(4636..4836)
                     /locus_tag="PLG24_06575"
     CDS             complement(4636..4836)
                     /locus_tag="PLG24_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24588.1"
                     /translation="MKKILTYIVLFVSIAAFSASVSSCSRKSGCPAYDEAVSKPKKNG
                     NFNKGKSQLFDKKMRKRMAQRG"
     gene            complement(5031..6089)
                     /locus_tag="PLG24_06580"
     CDS             complement(5031..6089)
                     /locus_tag="PLG24_06580"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HMZ24589.1"
                     /translation="MGSFIKGLGYYVPENIVKNEDLVKYMDTTDEWIQERTGIKERRY
                     ATRHKESTVSLGAEASRKAIADAGIQPSDIDFIIFATLSPDYFFPGCGVLLQRELGIT
                     DTECGALDVRNQCSGFIYALSVAHQFASSGMYRNILVVGAEVHSMGLDYTTRGRNVTV
                     IFGDGAGAAIVQPTAESGEGILATCMHSNGTHAEELAFINPGCHGGRYGGIENYGYLD
                     EKEYGGTFLTQKMMDDALIYPNMNGQLVFKNAVQKMPEAIMEVLNKAGYKPSDLKMLI
                     PHQANLRIAAFVQHTLRLNDDQVYNNIQKYGNTTAASIPIALTEAYQQGLFSSGDLVC
                     LTAFGSGFTWGAVLMRWA"
     gene            6607..>7143
                     /locus_tag="PLG24_06585"
     CDS             6607..>7143
                     /locus_tag="PLG24_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24590.1"
                     /translation="MKLLKPGKYLFGCIVCLLGMIPLTTFGQVPAPNLYCVKGDTLRW
                     DIPTVACGPVLSYEIFYSTNYNGPYQLLETITNLTQTEYIFSASPGGTLYYYMQTTAN
                     CSGQTALSSDTLDNASPLPVVIQSVSVAGNDIILSWSKGLSPETSAYVIYQGLGGGVV
                     KALDTVTTLIYTDTNRKTA"
BASE COUNT         1789 a         1668 c         1332 g         2354 t
ORIGIN      
        1 gacattctta tagttggatt catccatcac ataaattaaa tcaaatgttt caagatctgc
       61 ctttgtaaat tggcgtgcct tcaaccctgt gatgtcaata ttatgctctg acgcaatgcg
      121 cacactgttt ctatgtggag cctctccgat atgataatcg cttgtccccg cactatctac
      181 cttgatttta agaccggcac tttctgcttt agcccgcatg atgccttctg ccaagggact
      241 tctacagata ttgcccaggc agaccattaa aattttcata ggattaaaat tgttgtagtc
      301 taaactttat attgaaactc tttattattt atacaatatt aatattcaaa ttgtttcaaa
      361 attaatcaga atttaccatt tcaaggtaat aattttcttt ttgtcgcata actgccactt
      421 ccttttctgt tttatcgccg aatccgcata agtgaatgca cccgtggagc atcacccgat
      481 acagttctcg tgttccgcca tcatctgctt tacctgcatt ttctaaaact ctttcggtgc
      541 tgatatagat atcagattct actctatcat catcataatc aaaggttatt atatccgtat
      601 agtaatcatg gtcgaggtag ctccgattga tttccagaag atactcatca ttacagaaaa
      661 tgatattgac ataacctaca attttatgat ggtctgcaaa aactttaccg gctaccgctc
      721 tgaacttcgc tttcgaaaac ctgattgggg gatgttctcc ctcgctaaaa aaatgaattt
      781 tagttgtcgg cactactttt tgatattgaa tgtcatttcg acacaggagc cattgttttt
      841 aaaccgaata tcgtcactca atgagcgcat gatcagtact cctctccccc ccggtttttc
      901 aatattctcc ttgcagcaag gatcaggtac ttttgcaact tcaaatccgg ggccttcatc
      961 tttgatgcag atggtcagct tatcttcctt acaataggat tgcagtgtta cctccttgtt
     1021 ttcatcgatc tgattgccgt gaattattgc attattgacg gcttctgtca cgctgatcag
     1081 gattttcggg aacaactctt catcgagatt gagctctcgt tgcagggttt ttgcaaatgt
     1141 ctcaacttca cttacacttt tgagccggct tgatatcctg attacattta cttgtttcat
     1201 ctgcctgaaa gttgattata atattgttca accaatcgtt tgtaataagg tgtaagggca
     1261 ggatatacag gccggatatc atcaattcct gcttcccttt ttttgatata ttcctgcatg
     1321 gatgaaggaa tcttgttttg ttgctgttga gcctgtgtcg cctttcgttg ttcatccttg
     1381 tcctgttctc tttccgcacg ttcttcttcc aaaagtcttg tcatgatatc ctgctgtctt
     1441 cgcaacattt cattagtaag gcgtctgttg accagatcct tttcgatttg atccatggcg
     1501 tcgataatat cctgaatttg ctttttgccc tgaccattct gtcgcatctg agcatctcgg
     1561 tcctgaagca tcttacgtat tttagcctgt tgggcagcca actgtccaaa ttcctcactc
     1621 ccgggttttt taccattttt catttgctcc atctgctgct tcattccttc tgtcatcttt
     1681 tgctgaccct gggatatctt atccatctgg ggcatttcgc cgggtttgcc ggtcttccca
     1741 tcctggggat tacttcctcc gggtttgcta caactggcat tacccggcat agattgtgcc
     1801 tgcatctgca tttgctgcaa tgcctcactt agcataagtg caagatcatt gaggcttgtc
     1861 attgattgct gttgattgac agcagcctcc ggcttacgtc tgtcttcgag attatccata
     1921 gtcttgtcca ggttgtcatt gacttcagcg actttcttga tgataaacga ctgtatctgc
     1981 ataacccgct tactaagttc ctgcagactg tcatccacca tcttgaagtc agacttgatt
     2041 tttttctgtt ccctgttcag accgatgtat ctcggtgtgt tgatcatggt tgcattatac
     2101 tgtttgataa ggtcttcctg attaaatgac agaccaacta tattttccag caactgacgg
     2161 acggctttca tatccagctc tatctgttcc ttttcagatg ccgccatatc attctgcata
     2221 ttctgagcca tctgcttcat cttctggccg gctttctttt ggctcttaga agcggattta
     2281 ttttcttttt tctccaattg ttccttggca tcctgaaggt cctgctccgt actttgctgc
     2341 tcttcttttt gatcattcaa cttcttaggc gccttcaatt ccttgttttc tttctgtatc
     2401 tcatccattt tcttcttcac ctcttgaaag tccttattga tctggtcttg ttccttctta
     2461 agttgctctt gatccttttg attattctca gtctgctcag caagcttttc ttccttttcg
     2521 gccagttcat tcagtttatc tatctgatcc tggaccttag cttccagttc aagctgcttc
     2581 atcaattcca taagccgatc cagactctta tgctgctgtt cattttgttg agacatttgc
     2641 tccagttgtt tgagggactc ctttttatcc agtttatcca tcaactcccg gatcttatcc
     2701 atcaactttt gttcttccgg gttctgcatt tccttgatca tttcatcaag tttctcctgt
     2761 ttttccttca tctcagggtc aggctgcttt atctcttctt ctgccttgcg tttctcctcc
     2821 agttttttct gggtctcctt tagttggtct tgaagctcct tttgtttctc cagcaactta
     2881 ttcaactgtt ctttatcctg ccaattcaat gacttttgct gcagtaattt ttccctgagc
     2941 ttattgattg catcattgac ttttttcgat tccttcacca ttttttccag atcactttgg
     3001 atttccctgg tggacttatc gatatttttc tccatctcct taacagaagg cttcttaaag
     3061 ttcatcactg aagtcctggc agatttgggc cctttggcac catcattgtc gaatacctcg
     3121 aaaaaataag ttaattcatc acccggtttc aactttaacg attctatatc aaaaagatag
     3181 ctatagaatc cttgtttagt tgcgggtttc tgtagtggca gtgtcctggt tcttactttt
     3241 ccatcttcgg atttgatctg ttgcaccatt tgcagctttg taaagccata atcatcagag
     3301 acattgccac tgagatagac cagtttctgg ttggctgaat ccacatattg atcaagactg
     3361 attacagggt tttcatccat cacgacatgg atcgaatatg tcatgctgtc acttaccggg
     3421 agtgcctgat tgcctgtgaa gatcttataa ttttgatcct gtatggctgt cttcaaaaat
     3481 gtaaatctat tgtcgcccgc ccgttgtgcg ctcacggcat gattgccttc aaattccaac
     3541 tcaacccgat cggcaagtct ggtcctgata tcccagtata cttttgtgcc gaatgggacg
     3601 atgatgtctc cggagccttt gacgtattcc tcaggtcttc caatatagcc cggatagacg
     3661 agtttcaggt tgaggtctgt cattcccgct tttttcatta catccaatcg atacaccttg
     3721 ctattaaatc cggcagcctt cagtctgaaa tcaaaagcat cctggacatt agcaaaggtg
     3781 taggtgaaaa tgccccgact atccttgacc aacctgtatg gattatcatg atagaccaca
     3841 tcaatctgat ccggaatcga attcccctcc aatttgactt ccagtgtata gttatcaaac
     3901 tcggcgacct tcaggttttt gttcagtatc tggaaggtaa atggtgcctc ccgttcaaac
     3961 tccttatcgt tgttgatcaa tcgattgaca ggcttcttaa tcagggaggg ggcggtaaaa
     4021 agcataagaa gcagcgccaa taccggcggc acactatact tgagatattt tttattggag
     4081 ctcaggtcaa tggcattttt aaagggaatt ggctgcaact cttttgtttt ttgctctatg
     4141 cctgcgatca aaagggagga atcggggttg ttacgtgctt gctcattgag ttgcaataca
     4201 ttgagtaatt tgtccttgac attgccaaaa tgatctccta tgatggcagc cgcctgttca
     4261 ttggaaatgg tggagcctaa ccggaaaaac ttcatagccg gaacaatgat ccaccgtata
     4321 agagtaaaca gggaggcaag taagaatgag taaaaaagga acttgcggcc cgcagtcccg
     4381 aaatagaaaa aattctccag caaggctatt gccatgaaat atacggtaac aaagcccagc
     4441 aaataaagcg aaccacgaat aatcctgttc agataaaact tgcggataaa ttgatccagt
     4501 ttttgtatca atagattata ttgctttcct gactccataa ataatcaact ccaaattatt
     4561 ttaaccgaaa atccgacagt ttcgtttcag attataaaga tcaatgatgt gataaaaatt
     4621 aaattaagtc ccgaatcagc ctctctgcgc cattctttta cgcattttct tgtcaaaaag
     4681 ctgagactta cctttattaa aatttccgtt cttcttcggc ttcgaaacag cttcatcata
     4741 agcagggcat cccgatttcc ttgagcagga gctcactgat gcagaaaaag cagcaatgga
     4801 aacaaaaagg acaatgtacg tcagtatttt tttcataggc aaatgcttgt cagcgactac
     4861 atgttaaatt gaatttctcc ctataaaacg ctgttaaaat ctaaatagtt cgtaatcaac
     4921 atgattaacg gcataattaa cactttatct tcaagaaggt tcaatgatta attatttggt
     4981 aatctctatg caaatgctat tccgtaattc taatatagat ctaattggat ttatgcccag
     5041 cgcatcaaca ctgcacccca ggtaaagcca ctaccaaagg cagtaagaca tacgaggtct
     5101 ccggaagaga acaagccctg ctgataggcc tctgtcagtg caatagggat ggaagcggct
     5161 gtagtgttgc cgtatttctg aatgttgttg tacacctggt cgtcattgag tctaagggtg
     5221 tgctgaacaa aggccgcaat acgcagattg gcctgatgtg gaatcagcat cttcaggtct
     5281 gatggtttgt atcctgcctt attcaatact tccatgatgg cctccggcat cttctggaca
     5341 gcattcttaa acaccaattg tccattcata ttaggatata tcaatgcatc gtccatcatt
     5401 ttctgtgtca gaaaggtacc tccgtattcc ttctcatcga gataaccgta attttcaata
     5461 ccgccatatc ggccgccgtg gcaacccgga ttgatgaagg ccagctcttc cgcgtgcgtc
     5521 ccattactgt gcatacaagt agctaagata ccctctcctg attctgctgt tggttgtacg
     5581 atggccgctc cggcaccatc accgaagata acggtcacat tcctgcctct tgtcgtataa
     5641 tcgagaccca tggaatgcac ttccgcgccc accacaagaa tattcctgta catacctgaa
     5701 gaagcgaact gatgcgccac acttagcgca tatataaagc cggaacattg gtttctgaca
     5761 tccaaggcgc cacattcagt gtcagtgatg cccaactccc gctgtaacaa caccccacat
     5821 ccgggaaaga agtaatccgg gctcagcgta gcgaagatga tgaagtcgat gtcggatggc
     5881 tgaatccctg cgtccgcaat ggctttcctg gaagcctccg cccccaagga gaccgttgat
     5941 tccttgtgtc gtgtagcata tcgcctttcc ttaattcctg tcctttcctg gatccattca
     6001 tcggttgtat ccatatattt gacaagatct tcatttttta caatattctc cggaacgtaa
     6061 tatcccaatc ctttgataaa agaacccata tttgattatt tattttccca aagaaaatga
     6121 tttttaggca aatacagaag gatttcacac tattttagca agacgaaatg acaaatattt
     6181 attaatacga atatatataa tattgtatta taatcatata tcaggatgtt cgaaatatta
     6241 gacattgttg caagccccat ttcgtcatct catataccgg ctgctgaggg aggcgaattc
     6301 ttgcttcata ctcccgggac atactggtta tgtgggtaaa gtttgaaacg aatttgagag
     6361 ctcattgaat tctttaattg catctatacc aaatcatgga ttcctgctcc cttaggaaga
     6421 tttcctgcct gcccgaagat ttactcaaaa atagactgca cggcaggaag catatcttta
     6481 caaattatta taattctgaa aacataagga tttacaagac ttaattcaat tcaatccttc
     6541 aattaacata agttacaggg gaagtttatc gttttcatta tggaatttgg cataaatttg
     6601 tcccatatga aattattgaa acccgggaaa tatttgttcg gctgtatagt gtgccttttg
     6661 ggtatgatac cactaaccac ttttggtcag gtgcctgcac ccaatctata ttgtgtaaag
     6721 ggagacacat tgcgctggga tattcccacc gtcgcttgcg gccctgtcct atcctatgaa
     6781 attttctatt ctaccaatta caacggcccg tatcagctat tggagaccat cactaacctt
     6841 actcagacgg aatacatatt ttccgccagt ccgggaggta cactctatta ctacatgcag
     6901 actacagcca attgttccgg gcagactgcc ttatcatctg acaccttgga caatgcatct
     6961 ccccttccgg tggtaataca atccgtctca gttgccggca atgatatcat cctttcatgg
     7021 tccaaaggcc ttagtcctga gaccagtgcc tatgtcatct accagggtct gggaggggga
     7081 gttgtcaagg cattggatac cgtcaccaca ctaatatata ccgatacaaa caggaagacc
     7141 gcc
//
LOCUS       DAOQQN010000141         7130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4290_length_7130_cov_4.965230, whole genome shotgun sequence.
ACCESSION   DAOQQN010000141 DAOQQN010000000
VERSION     DAOQQN010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4290_length_7130_cov_4.965230"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(318..1073)
                     /locus_tag="PLG24_06590"
     CDS             complement(318..1073)
                     /locus_tag="PLG24_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HMZ24591.1"
                     /translation="MEPYVHTTIKEDRIEIEFFHPSGNSLPGESLSRLVSEIQHAEKA
                     KPNVILLKSGGERTFCAGASFDELTSITNEHEGLHFFSGFANVINAMRQSPKLILTRV
                     QGKAVGGGVGLISGSDHVVATKYASVRLSELAIGIGPFVIGPAVERKTGVSAFARMAL
                     TPDEWQTAQWARDHNLLQEVFEDIRQVDAYIEALIAKWNTYSPDAIRELKKVIWQGTD
                     HWDELLKERAAISGRLVLSQFTRDAIEAFRNKA"
     gene            complement(1074..3122)
                     /gene="paaZ"
                     /locus_tag="PLG24_06595"
     CDS             complement(1074..3122)
                     /gene="paaZ"
                     /locus_tag="PLG24_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HMZ24592.1"
                     /translation="MQIVQNYIAGHWISGDGDGQSLIHAISGEVFGSVSSKGLDFQSM
                     FDYGRNVGNKNLRRMTFQQRALMLKALALYLFDRKERYYEVSYKTGATRMDSWVDIEG
                     GIGNLFANASLRRKLPDQPFTIDGEYAQLSKGGSFIGQHILVPKLGVALHINAFNFPV
                     WGMLEKIAVNLLAGMPAIVKPAAITSFLTEAVFKDIIDSGILPEGALQLVSGSAHNLI
                     EYVREQDVVTFTGSAETGRKLKANPAIIDYAVPFNMEADSLNCCILGEDVEVGSVEFD
                     IFIKEVRREMITKAGQKCTAIRRIIVPEQKVDVVAEALKEVLSKVVIGDTREKEVKMG
                     SLVGKNQVEEVLSKVALLLKDADLVCGMSDHVNLFGEQVSGDAFMEPVLLKARNTDAN
                     SSVHEVEAFGPVSTIIGYKSLEEAIDLANKGKGSLVSSMVTYDDGIATDYVLGAAPYH
                     GRILVLNRDCAKESTGHGSPMPLLVHGGPGRAGGGEEMGGLRGIKHYMQRCAVQGSPT
                     TLTEITQVYQPGGKYKEPAKHPFRLHFEELTIGDTLITHKRTVTEADIVSFANVSWDH
                     FYAHTDVTSLDGTIFTERVAHGYFVLSAAAGLFVDPGKGPVLLNYGLEECRFLKPVYA
                     GMTIGVKFTVKEKVNQEKKSDDDIAKGIVKFYVEVYDETDETVAVATILTMIKKINQE
                     "
     gene            complement(3449..4888)
                     /locus_tag="PLG24_06600"
     CDS             complement(3449..4888)
                     /locus_tag="PLG24_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMZ24593.1"
                     /translation="MAEGQPGFKQSLKLTDATMLVAGSMIGSGIFIVSADTGRMLGSS
                     GWMMAAWLFAGILTLIAALSYGELAAMFPKAGGQYVYLREAYNPLIGFLYGWTMFTVI
                     QAGTIAAVAVAFAKFTSYFVPWFGEDNILFGIGSWHFKGSQVLAILSILVLSGINLRG
                     INVGKVVQTSFTLAKIIALFGLILLGLTLGYNADVFSANLKDMWHAGRWDGTGFVQVT
                     GSALLIAFGGAMVGTLFSSDSWNNVTFIAGEIKDPKRNIPRSLFLGTLIVTILYLLTN
                     LTYLALLPLQGSPDGVDAVSRGITFAVNDRVGTAAASLIFGASAAAIMSILIMISTFG
                     CNNGLILSGSRLYYAMAEDKLFFPKAGVLNDKAVPGFGIKAQAIWASALCLTGTYSDL
                     LDYCIFAALLFYILTIAGIFILRRKRPEIVRPYKAFGFPILPAFYIIAGLVIAVILLI
                     DKTENTLPGLVIVLLGIPVYFLLRKKLSN"
     gene            complement(4939..5322)
                     /locus_tag="PLG24_06605"
     CDS             complement(4939..5322)
                     /locus_tag="PLG24_06605"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HMZ24594.1"
                     /translation="MKKTQFEIEFLFRASPTMVYSFITSPTYLIRWFCDEADVESDVY
                     TFRWKDSEETAVLVDDFEDELLRFRWEDAVSDNEFLEFHIETSPVTEETILRIIGWAE
                     PSDHQFQIDFWTTQLKSLKKAMGEN"
     gene            5583..6452
                     /locus_tag="PLG24_06610"
     CDS             5583..6452
                     /locus_tag="PLG24_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ24595.1"
                     /translation="MRQLKITKSITNRESQSLEKYLQEIGKVDLLTPEEEVDLAKRIK
                     QGDQEALERLTKGNLRFVVSVAKQYQNQGLSLSDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNKVGSLNKINRAFSELEQQYEREPSAEELAG
                     ILDIPTEEVETTLGVAARHVSMDAPFVDGEDNSLMDVLENNSTPGTDTALEYFESLRR
                     EIERSLSTLTDRQCDVIKLYFGIGIDHPMSLEDIGDKFGLTRERVRQIKDKAINKLRS
                     VSRSKLLKHYLGN"
BASE COUNT         2172 a         1668 c         1348 g         1942 t
ORIGIN      
        1 ctataattcc tgaatatcct atccggatca atgagtttga gcccggtgaa atttcgtacc
       61 tatagagcgg accggcaatc aagtcttctg gccttcttcc tgccaatgct tctcccctat
      121 ggtgagaaat aaaactccaa aattccgtga gttttacgcg acattatcag gatccagccc
      181 atcagtgata aataaatacc aggaatggca atatggcaga gttggaataa ttacaaccct
      241 aaatagtgac tactaatcct ttcatattgc tttgaaatgg caagataaaa aagcgtcgaa
      301 ggatgtagga acgctattca agccttattc ctgaatgcct ctatcgcatc gcgtgtaaat
      361 tggctcaaaa ccagacgacc cgatattgca gctctttcct tcaacagttc atcccagtga
      421 tctgtccctt gccagataac tttctttaat tcacggatgg catccggact ataggtattc
      481 cattttgcaa tcaatgcttc gatatatgca tctacctggc ggatatcttc aaagacctcc
      541 tgaaggagat tatgatccct tgcccattgg gcggtctgcc attcatcagg agtgagcgcc
      601 atcctggcaa atgctgaaac accggttttt ctctccaccg ccggcccgat tacaaatggt
      661 ccgatgccaa tagccaattc actaagacgg acagaagcat atttggtagc cacgacgtga
      721 tccgacccgg atatcagtcc cactccacca ccaacagcct taccctgtac ccttgtcaaa
      781 attagcttcg gactttgacg catagcatta attacattgg caaatcctga aaaaaaatga
      841 agcccttcat gctcatttgt tatcgaagta agttcatcaa agcttgctcc ggcgcaaaac
      901 gtcctttcac ctccactttt caacaagatg acattcggct tggctttttc tgcatgctgt
      961 atctctgata ccaacctgct taatgattca cccggaagtg aattgccgga tgggtgaaaa
     1021 aattcaatct caatcctgtc ttccttaatt gttgtatgga cataaggttc catttattcc
     1081 tggtttattt ttttaatcat tgtgagaata gtagctacag caaccgtttc gtcagtttcg
     1141 tcatatactt ccacatagaa cttgacgata cctttagcga tatcgtcgtc tgactttttc
     1201 tcctggttga ccttttcttt aactgtaaat ttgactccga tagtcattcc tgcatacacc
     1261 ggcttaagga aacgacattc ttccaatccg taattcaaca ggacagggcc tttgccggga
     1321 tcaacaaaaa gccctgcagc cgcagaaagt acaaaataac catgtgccac cctctccgta
     1381 aaaattgttc catccaggct cgtcacatcc gtatgtgcat aaaaatgatc ccagcttaca
     1441 ttggcaaatg atacaatatc tgcttcagta acagttcttt tatgagtaat cagagtatct
     1501 ccgatggtca attcctcaaa atgcagtcgg aatggatgtt ttgccggctc tttgtatttt
     1561 cctcccggtt gatacacttg agtaatttca gtcaatgtgg tcggtgagcc ttgcactgca
     1621 catcgttgca tatagtgttt aatcccacga agccctccca tctcttcacc accaccggct
     1681 ctaccgggtc ctccatgtac taagagaggc ataggacttc cgtgtccggt gctctctttg
     1741 gcacaatccc ggttaagaac aagtatccta ccatgataag gcgctgctcc caagacataa
     1801 tcagtagcaa tcccgtcatc atatgtgacc atggaactaa ccagtgaacc ttttccttta
     1861 tttgccagat caatggcctc ctctaagctc ttgtaaccaa tgatggtcga cacaggaccg
     1921 aatgcctcca cttcatgtac gctgctattg gcatctgtat ttctggcctt gagtaaaaca
     1981 ggttccataa aggcatctcc acttacctgc tccccaaata gatttacatg gtccgacata
     2041 ccgcagacca gatcggcatc cttgagcagc aatgccacct ttgagagtac ctcctcaacc
     2101 tgattcttgc caactaaaga acccatctta acttctttct ctctcgtgtc gcctatgact
     2161 accttggaaa gaacttcctt caaagcttcc gccaccacat ccaccttttg ttccggtacg
     2221 atgatgcgcc tgatagccgt acatttctgt ccggctttcg taatcatctc acgtcgaacc
     2281 tctttaataa aaatatcgaa ttcgacactt cccacctcca catcttcacc caatatgcag
     2341 caattgagcg aatcagcttc catattgaaa ggtacagcat agtcgatgat ggcaggattg
     2401 gcctttagtt tacgacctgt ctccgcagag ccggtaaagg taacaacatc ttgttctctc
     2461 acatactcaa tcaggttgtg agccgagccg cttacaagct gtaaagctcc ctccggaagg
     2521 atgccactgt cgatgatatc cttaaacact gcttctgtca gaaaagaagt tatggctgcc
     2581 ggcttaacaa tagcaggcat accggcaagc aggttgacag caattttctc caacatgccc
     2641 cataccggaa agttaaaagc attgatatgc aaggccacac ccaatttcgg aacgagaata
     2701 tgctgtccaa taaaacttcc accttttgat agctgtgcat attcaccatc gattgtaaaa
     2761 ggctgatccg gcagcttacg cctcaaactc gcattggcaa acaggttccc gatgcctccc
     2821 tcgatatcca cccagctatc catccgggtc gctcctgttt tataggagac ctcataatac
     2881 ctttcctttc tgtcaaacag atataatgcg agagccttca gcatcaaggc tcgttgctgg
     2941 aaagtcatcc tgcgcaggtt tttattgccc acattgcgcc cataatcaaa catggactga
     3001 aaatccaagc ctttggagga gacagaacca aatacttcac cactgatggc atggataaga
     3061 gactgtccat cgccatcgcc tgaaatccag tgtccggcaa tataattctg aactatttgc
     3121 atcttaggaa tttaatttaa taaacggcct taagtaaccg tttctttaat acaaaggaaa
     3181 aaaattaaat ttttatcacc aaaagaaatt aagaagattg ggaaaatgac caccaaagac
     3241 aaatctagaa gggacaaaga tgataaaatc acgaaacaat aaaggtgcga atcacaaaag
     3301 aggtcgaagg aatctttaca actactttca acattgggag tttgattaca tcaggtatca
     3361 atgatacgat gcttttaaga gcacatgtat cttacttcaa aaatttgttg ataaaataac
     3421 tgcttgaaga gttaattggc aacacaattc aatttgataa tttttttctc aataaaaaat
     3481 aaaccggaat tcccagtaaa acaatcacca aacctggcaa ggtattttca gtcttgtcta
     3541 tcaataatat cacggcaata accaaaccgg caatgatata gaatgctggc aggatgggga
     3601 atccgaaagc tttgtatggt cgtacgattt caggcctttt tctgcgcaaa atgaatatac
     3661 ctgcaattgt caatatataa aacaagagtg cagcaaagat gcaataatcc agcaagtcgg
     3721 aataagttcc tgtgagacaa agtgcagaag cccatattgc ctgagctttt atgccaaagc
     3781 ccggaaccgc cttatcattc aaaactccgg cctttggaaa aaacaattta tcttcagcca
     3841 tagcataata taatctactt ccggacaaga tcaaaccgtt attgcatcca aaagtagaaa
     3901 tcattatcag gatgctcatg atagcagcag cagatgctcc aaaaatgagt gaggcagcag
     3961 ctgtacccac cctatcattg actgcaaagg ttattcccct gcttactgca tcaacgccgt
     4021 caggacttcc ctgtagtgga agcagagcga gataagtcaa attagtcaaa agatataata
     4081 tagtcactat caaagtgccc aagaataagc tcctcggtat atttctcttt ggatccttaa
     4141 tctcgccggc aataaaagta acattgttcc aggaatcgct ggaaaacagc gtacctacca
     4201 tggcacctcc aaaagcaatt agcaatgcgc tgcccgtaac ctgaacaaaa ccggtgccat
     4261 cccaccgtcc ggcatgccac atatctttca gattggcaga aaaaacatca gcattgtatc
     4321 ccagtgtaag accaagaagg atcaaaccga aaagagctat gattttcgca agtgtaaagg
     4381 aggtttggac aaccttaccc acattaatcc ccctcaagtt gatacccgac aggactaaaa
     4441 tactcaaaat ggccagtacc tgggaaccct taaaatgcca gctacctata ccaaaaagta
     4501 tattatcctc accaaaccat ggtacaaaat aggaagtaaa cttggcaaag gccactgcaa
     4561 cagccgcaat ggttcctgcc tgaatcacgg taaacatagt ccatccgtag agaaagccaa
     4621 tgagaggatt gtatgcctct cttaaataaa cgtattggcc accggcttta ggaaacattg
     4681 ccgccagctc gccataactc aaagctgcta tcaaagtaag aataccggcg aacaaccagg
     4741 ctgccatcat ccaacctgag ctacccagca tacggcccgt atcagcgctt acgataaaaa
     4801 ttccggatcc tatcatcgac cctgcaacca acatggtggc atcggtcaac ttgagtgatt
     4861 gtttgaagcc cggttggcct tctgccatat acttgggagt tagttgtgta aaaaatgata
     4921 cttttaataa caaagggatt aattctcgcc catagctttt ttgagcgact tcaattgcgt
     4981 agtccaaaaa tcaatctgaa attgatgatc acttggttca gcccacccta taatcctgag
     5041 gatcgtctct tcggtaactg gggaagtttc aatatgaaac tccaaaaatt cattatcgga
     5101 tactgcatct tcccaacgga aacgcaatag ctcatcctca aaatcatcaa ccaaaacggc
     5161 agtttcttcc gaatctttcc atctgaaagt atatacatca ctttcaacgt ccgcctcatc
     5221 gcaaaaccat ctaatgagat aagtcggcga tgtaataaag gaatagacca tcgtcggtga
     5281 tgccctgaaa agaaattcta tttcgaactg cgtttttttc atttccaatt aaaattattt
     5341 tacctcttaa aaaaattact ttttgtactg tccaaaagcg gcaaatatta aaattcgggg
     5401 gtaaaataaa tattatttta atatgcaaat ggttttttag gccaaaatat tattttttcc
     5461 actttattca ggcatagtgg aacttttgat caataataaa cattatatca tttttagatg
     5521 ttacagactc aaaaacgcga ggaaactcta tctttgcgtc cgtttttaaa taaaatcttt
     5581 taatgaggca gttaaaaatt accaagtcaa tcaccaacag agagagtcaa tcccttgaaa
     5641 aataccttca ggaaataggc aaagtggatt tgctaacgcc tgaggaagaa gtcgatctgg
     5701 cgaaacgcat caaacaaggc gatcaggagg cattggaaag attgaccaag ggtaaccttc
     5761 gttttgtagt ttctgttgca aaacaatatc agaatcaggg actttcatta agcgacctta
     5821 tcaacgaagg gaacctcggg cttatcaaag ccgctcagcg ttttgatgaa accaggggat
     5881 tcaagtttat ttcctatgct gtatggtgga tcaggcagtc tatcctccag gcacttgcgg
     5941 aacaatcacg tatagtccga ctcccgttga acaaggttgg ttcactcaac aagatcaaca
     6001 gggcattctc cgagctggag caacaatacg aaagggagcc atcagcagaa gaattggcgg
     6061 gaatactcga tataccgacg gaagaagttg agacaactct tggggtggct gcacgacatg
     6121 tatccatgga tgctcctttt gtggatggag aagacaattc attgatggat gtcctggaaa
     6181 acaattccac accgggcacg gatactgctc ttgaatattt tgaatcactg agaagggaga
     6241 ttgagcgctc tctgagcaca ttgacagaca ggcagtgtga tgtaatcaaa ctctatttcg
     6301 gtataggtat cgaccatcca atgtctctgg aagatatagg tgacaaattt ggcctgactc
     6361 gggaaagggt tcgccaaata aaggataaag caatcaacaa gttgcgttca gtttcacgca
     6421 gtaagttgtt gaagcattat ttaggcaact aatacaaatt tttatatcgg ttgctgtacc
     6481 ggttataact gaaagtacaa ggtagttagt tacaaaactt ttatagatta aagtctttgt
     6541 ttacttcctt gtactttttc aattctcagg atcttttacc caggtaaaat tgatggaata
     6601 tgggtaatga gttctctccg aactaggtag atgtttaata gccgatgtcg caccaatatc
     6661 gggcatgatc attacttcat cctaaaaact tacccaaatt atgtcgtttt attttttaac
     6721 atccacataa aaggctgatg atagctggtt ttcaagttat tactttccta cctacttcgt
     6781 tttttccggc aacaactcaa gcagaacacg aaggacgcga aaacaaaaga tgtgagagcc
     6841 aatgctctcc ccttgaacta ggtagatgat taatagccgc aatggcaatg atatagggca
     6901 tgatcattac ttcatcctaa aaactcaacc aaatcaagtc gttttatttt tgacaacctc
     6961 atgaaaagct gatgatagct ggttttcaag ttattacttt cctacctact tcgttttttc
     7021 cggcaacaac tcaagcagaa cacgaaggac gcgaaaacaa aagatgtgag agccgatgcc
     7081 cttcccttga actaggtaga tgattaatag ccgcaatggc aatgatatcg
//
LOCUS       DAOQQN010000142         7121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4304_length_7121_cov_5.292811, whole genome shotgun sequence.
ACCESSION   DAOQQN010000142 DAOQQN010000000
VERSION     DAOQQN010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4304_length_7121_cov_5.292811"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLG24_06615"
     CDS             <1..297
                     /locus_tag="PLG24_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ24596.1"
                     /translation="QVNEEYRQRREKAFNVLELLNCTYAVDQCGMFVWARIPTWYKDG
                     YELSDKILYHAGVFITPGGIFGNAGDKYIRISLCSSVSVLEEACRRIDSLKKQR"
     gene            297..1148
                     /locus_tag="PLG24_06620"
     CDS             297..1148
                     /locus_tag="PLG24_06620"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HMZ24597.1"
                     /translation="MSQRKQIAIVGTGLIGGSLALRLHEKKLSSHLIGVDNDEKHAEK
                     AIAMELVDEILPLEEAIQRSEVIILAIPVDAIKRELSGILDRIDGQIVIDLGSTKQQL
                     IESVKNHRNRGRYVAAHPMWGTEYSGPESALREAFTAKAVVICDPDACDADALVWTRH
                     MFEKIGMRILDMEAEAHDLHAAYVSHISHVTSFALANTVLEKEKEDNAIFNMASGGFE
                     STVRLAKSNPLMWLPIFLHNRVNVLDVLNEHIGQLEKFRDALYSGDESKLIHWMENAN
                     RIRRIVG"
     gene            1185..2285
                     /locus_tag="PLG24_06625"
     CDS             1185..2285
                     /locus_tag="PLG24_06625"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HMZ24598.1"
                     /translation="MNTKTTEQTTMTTMDALWKKRPIIISGPCSAETEEQMLATARRL
                     ADTGKVDMLRAGIWKPRTKPGLFEGVGAKGLPWLKKSGDITGLPVTVEVANAKQVQDA
                     LAFEIDVLWIGARTTVNPFSVQEVADALRGVDIPVLIKNPVNPDPELWSGAVERIARA
                     GINRIGLIHRGFTSYGNSEFRNAPMWHLAIDMKLRYPDMLFINDPSHICGRRDNLREV
                     MQKAIDLDYDGFIIESHCDPDHAWSDASQQVTPETLSLMLDQIKWRKEDTSSAEFHIA
                     LEKLRNQINQLDDELMQILSRRMKIADQIGRYKKDNNITILQTTRWNEVMDRAVSGGA
                     GLGLSKEFILRYFDAVHIESINHQKRIMDGEE"
     gene            2343..2843
                     /locus_tag="PLG24_06630"
     CDS             2343..2843
                     /locus_tag="PLG24_06630"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HMZ24599.1"
                     /translation="MNERPKIKLEQTSIDKTFEILGWTSVIAIWILTITNYSNLPEII
                     PTHYNGAGQPDGFVGKANILTLPLIATVLFVGLTILNKYPHIFNYPTGVTQENALRLY
                     TNATRMIRYLKFIIVAIFGLITFKTIQNVNGEAKGLGNMFLPVSMGLIFIPLAYFLLK
                     SFRTTK"
     gene            2843..2965
                     /locus_tag="PLG24_06635"
     CDS             2843..2965
                     /locus_tag="PLG24_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24600.1"
                     /translation="MTERESMEFGGYCKNKDHLLWNQQQGEMKVRNGQTISTAS"
     gene            3084..4358
                     /gene="aroA"
                     /locus_tag="PLG24_06640"
     CDS             3084..4358
                     /gene="aroA"
                     /locus_tag="PLG24_06640"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HMZ24601.1"
                     /translation="MKSIIKPSRLAGTITAPTSKSSMQRACAAALLNKGCTIIRNPGL
                     SNDDKAAVEIIQALGADVRISKGEMIVKSNGINPISKEIHCGESGLGIRMFTSIAALS
                     EKEITITGSGSLMSRPMDFFDEILPQLGVKVESNGGKLPLKVRGPLVPGNITVDGNLS
                     SQYTTGLLMAYAAAGAKGVSIHVRNPTSKPYIDLTLEVMRSFGMEVPENRHYEEFVFH
                     GKPEKNTAPGQCQYTVESDWSGGAFLLVAGAIAGPITVCGLRQESTQADKAIMKALKS
                     AGAGIKVHEDKIQIYPSELKGFLFDATDCPDLFPPLVALAAYCRGETIIRGAERLTHK
                     ESNRALSLQSEFGKMGVVIDLAGNNMTIHGSCEVQGAEVSSWHDHRIAMACAVAALKA
                     KGGMTITDAEAVGKSYPGFFEDMRTLGADLEA"
     gene            complement(4631..5917)
                     /gene="fucP"
                     /locus_tag="PLG24_06645"
     CDS             complement(4631..5917)
                     /gene="fucP"
                     /locus_tag="PLG24_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HMZ24602.1"
                     /translation="MANPTDTTPTTSSSTKGYLLPLVFITSLFFFWGLIHNIDPTLIA
                     HLKKTYTLTNLQSSLIDFSVFIAYFLMAIPAGLMMRRWGYKAGIVFGLLLFAGGAFLF
                     LPAADTHQYVYFLGALFVVASGLTFLETAANPYVTVLGPPDTSSFRLNLSQSFNGIAA
                     VIAPIIGKYTILTHAADVSNEDIASMDATAKQEFIRQASESVKGPFMILGFIILAVAI
                     IFLFLKLPEITEEKSVKKRSFLEVLKVKHTRNAVIAQFFYVGAQICFASFFLVYAEKA
                     SGLDHLRASDYLAAYGLLFMLGRILGTIILKFVAAPRLLFIYALSASALTVIAIFGYG
                     AYTIYCMLGIAFFLSIMFPTIFALGVAGLGEDTKTASSLIVMSIVGGAILPLGLGYLA
                     DITGNIQYGYLVPLICLLVVALFGLRGYKPQQGLVT"
     gene            6081..6956
                     /locus_tag="PLG24_06650"
     CDS             6081..6956
                     /locus_tag="PLG24_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24603.1"
                     /translation="MDKIALRNGILAFILVLLLMPIGHAIMILNEVLLGDAKYYGAIA
                     MGFLGVALIVIGLLKNGNPTFATLMGFLGGVLVWTGWVEFSFMWVAEKNNVANLMENG
                     NIVTKREYLVMLSSMGLLCTTVLFYLFSRSNCTFFIWFQKVLGFRNQILTQTGYKKPF
                     SIIVFTETIMLLWFFYILLLAIYDDHIAGDRHWITYTVAWGSLFWSLYLINRLVRIRS
                     FDYAVRYAVPTVIIFWNFVEILGRWDLFKEIWIHPMEYWLEVLLLCLAFVTLMILFIV
                     NPTFHRKGNKETAAT"
BASE COUNT         2155 a         1381 c         1771 g         1814 t
ORIGIN      
        1 caggtgaatg aagaatatcg gcaacgccgg gaaaaagctt ttaatgtgct ggaattattg
       61 aattgcacct atgctgttga tcaatgcggc atgttcgtct gggcaaggat acccacctgg
      121 tataaggatg ggtatgaact gagcgataag attttgtatc atgcaggtgt attcatcaca
      181 ccgggtggca ttttcggcaa tgccggggac aaatacataa ggattagcct ttgttcgtcg
      241 gtttcagtat tggaagaagc ctgtcgaagg atagattcac taaaaaaaca acgatgatga
      301 gtcaaagaaa gcaaattgcg attgttggaa ccggcctcat cggtggttca ctggcgttgc
      361 gacttcacga aaaaaagctt tcatcccacc tgataggcgt ggataatgat gagaagcatg
      421 cagaaaaggc tattgctatg gagttggtgg atgaaattct gcctttggag gaagcaatcc
      481 agaggtcgga agtcatcatc ctggcgatac cggtagatgc cattaagcgg gaattgtccg
      541 ggatattgga tagaatcgat ggtcagattg taatcgatct tggatcgacc aagcaacaac
      601 tcattgagtc tgttaagaac catcgcaatc gcggcagata tgttgccgct caccctatgt
      661 ggggaacaga atacagtggg cctgaatccg ctttgaggga ggcatttact gcaaaagccg
      721 ttgttatttg tgatccggac gcatgtgatg ccgatgcctt agtgtggaca aggcatatgt
      781 ttgaaaagat cggcatgcgt attctcgata tggaagccga agcgcatgac ctgcatgctg
      841 catatgtcag tcacatctcg catgtaacat cctttgccct ggccaatact gttctggaga
      901 aggaaaagga agacaatgcc atcttcaaca tggcttcagg cggatttgaa agtacggttc
      961 gattggcaaa aagcaaccca ctgatgtggt tacccatctt ccttcataac agggtgaatg
     1021 ttttagacgt attgaacgag cacataggtc agctggaaaa attcagggat gcgctatatt
     1081 caggcgatga gtcgaagttg atacactgga tggaaaacgc aaacaggata aggagaattg
     1141 tgggttgaca taaatcccgc attaataaca aaaaaacaaa aaacatgaac acaaagacaa
     1201 cagaacaaac aacgatgaca accatggatg cgctatggaa aaaacgcccc ataatcatca
     1261 gcggcccgtg cagcgctgaa accgaagagc aaatgcttgc tacagcgcgc cggctggctg
     1321 ataccggcaa ggtggatatg ttgcgtgccg gcatctggaa gcccagaacg aagcccgggt
     1381 tattcgaggg cgtgggcgca aaaggcctcc cttggctgaa gaaatcggga gatattacag
     1441 ggttgcctgt gaccgtagag gtagccaatg ccaaacaggt tcaggatgca ttggcctttg
     1501 agattgatgt gctctggatc ggagcgagga caacagtcaa tccctttagt gtacaggaag
     1561 tggcggatgc cttacgagga gtggatatac cggtattgat taaaaatccc gtcaatcctg
     1621 accccgaact ttggagtggc gctgtggaaa ggatagcaag ggccggcatc aacagaatag
     1681 gactgattca cagaggattc accagttatg gcaactcgga attccgcaat gcacccatgt
     1741 ggcatcttgc tattgatatg aagttgcgat acccggacat gttgtttatc aacgacccat
     1801 ctcatatctg cggccgtcgt gacaatctca gggaagtcat gcaaaaggca atagacctgg
     1861 actacgatgg ttttattata gaaagtcatt gtgatcctga ccatgcatgg agcgatgcct
     1921 cgcaacaggt gacacctgag actctgagcc tgatgttgga ccaaatcaaa tggcggaagg
     1981 aagacaccag ctcagcggaa ttccatattg ctctggaaaa acttcgcaac cagatcaatc
     2041 agctggatga tgaactgatg cagattctca gtcgcaggat gaagatagct gatcaaatag
     2101 gccgttacaa gaaagataac aatatcacca ttctgcagac gacacgttgg aatgaagtga
     2161 tggaccgagc ggtatccggt ggagcaggtc ttggtttaag caaagaattt atcctacggt
     2221 attttgatgc agtccacatc gaaagtatta accatcagaa acgtataatg gatggagagg
     2281 agtgatgggt aacatggtag atataaccca atcctggata ttcggtatat aatggtatga
     2341 ttatgaatga aaggccaaaa attaaattgg aacagacatc gattgacaag acctttgaaa
     2401 ttcttggttg gacctctgtt attgcaattt ggattctgac tattacaaac tattcaaact
     2461 tacctgaaat tattcctacc cattataatg gtgcaggaca accggatggg tttgttggga
     2521 aagcgaacat tttgacgcta cctttgatag caactgttct attcgttggg ctgacaatac
     2581 taaataagta tcctcacata tttaactatc caaccggtgt aactcaagaa aacgcattaa
     2641 ggttgtatac aaatgcaaca agaatgatta gatatttaaa attcataatt gttgcgattt
     2701 tcggattaat tactttcaag acaattcaga atgtgaacgg tgaagcaaag ggactaggaa
     2761 atatgttttt accagtgtca atgggactga tttttattcc tttggcgtat tttttattaa
     2821 aatcatttag gacgacaaaa taatgacaga gcgagaaagc atggaatttg gtgggtactg
     2881 taagaataaa gaccatctgt tatggaatca gcaacaggga gaaatgaagg tgcgcaatgg
     2941 acagactatt tctactgctt cctgaatatg tggatagtta cgaagcaatc aattcaatct
     3001 ttgaaggatg aagatgctga ccaacgccaa aaacatttac ctttgaaata aacgataaca
     3061 acaataacag tcaacaagaa tcgatgaaat caatcataaa gccatcgcga ctggccggta
     3121 ccatcacagc acctacttcg aaaagctcaa tgcagcgtgc ttgtgcagcg gcactattga
     3181 acaaaggttg cactataatc cgcaatcctg gcttgagtaa tgacgataaa gcggcagtgg
     3241 aaattattca ggcacttggt gctgatgtcc gaatttcaaa aggcgaaatg attgtcaaaa
     3301 gtaacggtat aaatccgata agtaaggaga tacactgtgg cgaaagcggc ctgggtatcc
     3361 gcatgtttac ttcaatagcg gcattatctg aaaaagaaat aaccataaca ggaagtggca
     3421 gcctgatgag ccgaccgatg gacttctttg atgaaatact gccgcagctg ggagtgaagg
     3481 tcgaaagcaa cggcggcaaa cttcccttaa aagtacgagg gccgctggtt cccggaaata
     3541 tcaccgtaga tggcaatctt agttcgcagt ataccacggg attgttgatg gcctatgcag
     3601 cagcaggagc aaaaggcgta tccattcatg tcagaaatcc aaccagcaag ccatacatcg
     3661 accttacctt agaggtgatg cggtcttttg gcatggaagt acctgaaaac aggcattatg
     3721 aagaatttgt attccatgga aaaccggaaa agaacacggc cccgggacaa tgtcaatata
     3781 ccgtggaatc ggactggagt ggaggagcct tcttgttggt tgccggagca atagccggac
     3841 cgattacagt atgtgggtta cggcaggaat caacccaggc cgacaaggca attatgaaag
     3901 ccttaaaatc agcaggagcc ggaataaaag tccatgaaga caagatacag atctacccat
     3961 cagaactgaa aggcttcctg ttcgatgcaa cggattgtcc cgatcttttt ccaccgttgg
     4021 tggcattggc ggcgtattgc cggggtgaga ccatcatacg aggtgccgaa cgactgacgc
     4081 acaaggaaag caaccgtgca ttatcactac agtccgaatt tggtaagatg ggtgttgtca
     4141 ttgaccttgc cggcaacaat atgaccatac atggcagttg tgaggtgcaa ggagcagagg
     4201 tatcttcctg gcatgatcac cgaatcgcca tggcgtgtgc ggtcgctgca ttaaaggcga
     4261 agggaggtat gaccatcacg gatgccgaag ccgtcggcaa atcctatccc ggcttttttg
     4321 aggatatgag gacattgggg gcggatctgg aggcataaaa ttttgatttg acaaggattc
     4381 cctgctattt ataattattc ggggggtaga ttcatatatt tcaatcgaat tttcgcagag
     4441 ccataccggg tagttttcca ataccgaccg accgtcagat ttttaaagaa gtatacgcaa
     4501 aatagaaaga caagaaagtt tccggataaa gaaagctttg taaagcaacg gattatctaa
     4561 atatattcag tatacccatg ctgtagtgtg agtttggaag gaaggcgtga agaggagaaa
     4621 aggaaagatt ttatgtaacc aatccctgtt ggggcttata accccgcaat ccaaacagag
     4681 ccactaccag aaggcagatc agaggaacca aataaccata ttgaatattg cccgttatat
     4741 cggccagata gcccaagcct aagggtagga tagccccgcc cacaatggac atgacgatta
     4801 ggctggaagc ggttttggtg tcctcgccca atcctgcgac accaagggca aagatagttg
     4861 gaaacatgat ggataggaaa aaagcaatgc ccagcataca gtagatggtg taggcaccgt
     4921 aaccgaaaat agctatgacg gtcaatgccg aagccgacag ggcataaatg aataatagcc
     4981 ggggggcagc aacaaacttc aatatgatag tgcccaaaat ccggccaagc atgaaaagca
     5041 ggccataggc ggccaggtag tcgcttgccc tgagatggtc aagaccggaa gccttttctg
     5101 catacacgag gaaaaaactg gcaaagcaga tttgtgctcc tacgtaaaaa aactgggcaa
     5161 ttaccgcatt tcgggtatgt ttgaccttca aaacttccag aaaacttctc tttttaactg
     5221 atttttcttc tgtgatctcg ggaagtttca aaaacaggaa gatgatggct actgccagga
     5281 tgatgaagcc caatatcata aaaggcccct ttacactttc agaagcctgt cggataaatt
     5341 cttgtttggc cgttgcatcc atcgatgcaa tgtcttcatt actgacatcg gcagcatgtg
     5401 tgaggatggt gtatttgccg atgataggag caatgactgc cgctatgccg ttgaatgact
     5461 gagacagatt gagtcggaaa gaggaggtat cgggtggacc cagcacagtg acataaggat
     5521 tagctgcggt ttccaggaag gtaaggccgc tggccacaac aaaaagtgcc cccagaaaat
     5581 acacatattg gtgggtatcg gcagcaggca ggaagagaaa ggcaccaccg gcaaagagca
     5641 ataaaccaaa cacaatgccg gccttatagc cccatcggcg catcatcagc cccgccggta
     5701 tagccatcag aaaataagcg atgaagaccg aaaaatctat cagggatgat tggaggttag
     5761 tcagggtgta ggttttctta agatgggcga ttagggtcgg atcaatatta tgaattagcc
     5821 cccaaaaaaa gaaaagactt gtgataaaga ctaaaggcag cagatagccc ttggttgaag
     5881 aagatgttgt gggagttgta tcggtagggt tagccatttg tcaggtttta tcttatcaaa
     5941 aataatcatt aggtcgaaat tggtatcatt ttattatccc gatgggaact ttgtggcaaa
     6001 accaaaatat aaaaaacaac accaatgtta tttagactaa atataaatta tatctttgct
     6061 cattaatatt attaaaggat atggataaga ttgcattgag aaacgggatt ctggcattta
     6121 ttctggtact tttactcatg cctatagggc atgccatcat gattctcaac gaggtattgc
     6181 ttggtgatgc caagtattat ggagctatag cgatgggctt tctgggggtg gctttgattg
     6241 taattggctt gcttaagaac ggcaatccga cttttgccac attaatgggc ttcctgggcg
     6301 gtgtacttgt gtggaccggg tgggtggaat tttcctttat gtgggttgct gagaaaaata
     6361 atgttgccaa tctgatggag aatggaaata ttgtcacaaa gcgggaatac cttgtcatgc
     6421 tttcatccat gggtctgcta tgtactacgg tgctgttcta tcttttttcc aggtccaatt
     6481 gtactttttt catctggttt cagaaagttt tgggtttcag aaatcaaatt ctgacgcaaa
     6541 cgggttataa aaagccattt tccattattg tttttacaga gactatcatg ctgctctggt
     6601 ttttctatat tcttttactc gccatatacg atgatcacat agccggtgac cgccactgga
     6661 ttacctacac tgtcgcatgg ggctcattgt tctggtctct ttacctcatc aatcgacttg
     6721 taaggattcg ttcctttgat tatgccgtaa ggtatgcagt gcctacggtc ataatattct
     6781 ggaattttgt agagattctt ggaagatggg atcttttcaa agagatttgg attcatccga
     6841 tggagtattg gttggaagtt ttgttgttat gtttggcctt cgttacattg atgattttgt
     6901 ttattgtcaa tcctacattt caccgaaagg gtaataagga aacggcagcg acctgataat
     6961 ggaaggccgg ttaaatttct gattattgga tagaataggc ttagcgggca ggtgtggagg
     7021 cttgtggtaa atggtggtta gcgtggtcac actgaagtag gtagataaat aatacgaaaa
     7081 tgaatattta aatagaccaa aatgaatcta aagaaatata t
//
LOCUS       DAOQQN010000143         7115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4312_length_7115_cov_5.509773, whole genome shotgun sequence.
ACCESSION   DAOQQN010000143 DAOQQN010000000
VERSION     DAOQQN010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4312_length_7115_cov_5.509773"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="PLG24_06655"
     CDS             complement(<1..1141)
                     /locus_tag="PLG24_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HMZ24604.1"
                     /translation="MADNIYDAIVIGSGIAGGWAAKELAEKGLKVLMLERGRNIEHIK
                     DYVNANKDPWVFEHRGERTREMINTHPVLKRDYPLNEKVLDMWVNDLESPYIEDKRFD
                     WYRGYHLGGRSLLWGRQSYRWNKWDFEANGKEGIAVDWPIRYEDLAPWYSYVERFAGI
                     QGSYEALEVLPDSEFLPAMEMNIVEKDLARNLKEKYKGSRHMFIGRSANITKPMPEQN
                     RVNCQYRNKCWLGCPYGAYFSTQSSTLPAAKATGNLSLEVNKIVTRLLYDKDSKKATG
                     VETLDAINNQTYEYKAKIIFVCASAFNSTWILMNTATDIWPDGLGSSSGELGHNVMDH
                     HFRVGAGGSVEGYEEYIEYGRRSTGIYIPRFQNIYGDKRNYLRGYG"
     gene            complement(1316..2068)
                     /locus_tag="PLG24_06660"
     CDS             complement(1316..2068)
                     /locus_tag="PLG24_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ24605.1"
                     /translation="MNNLQLLLVEDEVIIAENIRQSLVDLGYECVTVCNTFDDAIEVI
                     EKGEIDLAIMDINLDDAEGRTGLDLASRLKKVKPTPFIFITAYSDKETVESASKLGPS
                     AYLVKPVNDKTIFSTIQIAMQNFNSNQTPVHSDSPESPAQYFFTKIGNKTFKIFWSEV
                     YLIRAIKNYVEIKAFGYTQTFLIRTSLTNCLESILPDNQKNYFVRINRAEAIRSEIVR
                     SYDNQKIYTNYGEFKLSSDHVIELQGKMRNIN"
     gene            complement(2065..3705)
                     /locus_tag="PLG24_06665"
     CDS             complement(2065..3705)
                     /locus_tag="PLG24_06665"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMZ24606.1"
                     /translation="MRSNIYLPLLGILFVLTISSCRDDNGVCISDPECLRLRFRYYQS
                     MGDSLSKLKSSTTAIIDSKVYYDSAALIAHGLADSSLLATNYLSLGRMYYDWGTQPDM
                     AIEYIEEAGKLFEEDKDYENHYYESKLLLCKSLLERSESSKVLTILEQLLEKISNSSL
                     DMTTLSGIYLDLGMVALMCHDTILVKKALQGIEILKLKQPDLAVPDPMKYYLLHSEAG
                     FFAKEDNLQPLDSLDKLYSTLGIMQDSLFYAGRLTSLMERSGRFEKAFHYQTILTNLL
                     LHENARYDKLAKQKIEFAERSLDHTRNIEKKTDQLWVIIICWLVLFAIASSYIIIFLR
                     RGSTKYYKLSKDLEFANNNAAILYKELHHRIKNNLHLIFSLLQMQERKTDDKVTKLNL
                     RDARLRIESIAIMHEEMMNLDGKIDFKDFIFRLIQLIINCFSLSTHIISEINFEKVEI
                     PHKYNFPIALIINEWISNSIKHAILGDKYLEITLNVFQENDQVVIEYYDNGDQKAETE
                     PSSGLGTNIVQLLMKQIKAELFKGHRGLYHYKIKMGRI"
     gene            complement(4120..5013)
                     /locus_tag="PLG24_06670"
     CDS             complement(4120..5013)
                     /locus_tag="PLG24_06670"
                     /inference="COORDINATES: protein motif:HMM:NF018431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator N-terminal
                     domain-containing protein"
                     /protein_id="HMZ24607.1"
                     /translation="MPVLTHIPPAPPLTHEGSLKTLVENRTCYTLDNYELSLYETYIP
                     AKSVYFKFDQFMVASMLRGKKVLHRMEKDDIFFKPGQTAIVPPNAAVNIDFPDTNLKN
                     PTQCLTLAIDNHKVAKTLDFLNERYPREGNDNFWKLNFDNFIFQNDEEIATILGKIIS
                     ICQNDTFFKDVLADLSIQELLVKIVQSQSLQSAEAGDINKLSPQLQQVITLIRDNLNT
                     RLDLKFILKKAGMSNSALYRLFKTELGISPREFIMTERIRLAKQFSSLVTRIPISKMS
                     VTKPVLKIVTTLSVLLNIMKV"
     gene            6145..6780
                     /locus_tag="PLG24_06675"
     CDS             6145..6780
                     /locus_tag="PLG24_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24608.1"
                     /translation="MYIFNNFIASLLLLVPAWFANISEDYTKPNLQKTYKILFVGNSL
                     SGTNDLPALFENKAKSKDIDVEVHSLISPAHSLEDHLLGNRIQLTIRNGHFDYVIIQQ
                     GPSPGSSGRDVLIKDGGKIQRMCKQAGSKMAYLMVWPSKKNYNRFDTVISSYTQAAKK
                     NNAILIPAGQVWKDYIDATNDYSYYGSDGFNPSFKGSEVAAEVIFNTLFGK"
     gene            complement(6797..>7115)
                     /locus_tag="PLG24_06680"
     CDS             complement(6797..>7115)
                     /locus_tag="PLG24_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HMZ24609.1"
                     /translation="LDASRCISYLTIELKDEALPDEFKGKMEDWVFGCDICQQVCPWN
                     KFSTPTREADFTPVSGIAEIMPEDWQQMTPERFESIFFQSPIKRTGLEGMKRNVQFVR
                     EEH"
BASE COUNT         2144 a         1547 c         1240 g         2184 t
ORIGIN      
        1 atccatatcc acggagataa ttgcgcttat ctccataaat gttttgaaat ctgggaatat
       61 agatgccggt tgaacgacgt ccatattcaa tatattcttc atatccttca acagagccgc
      121 ctgctccgac tctgaaatga tggtccatga cattatgccc caattcgccg ctactactgc
      181 ccagaccatc cggccaaata tccgttgcgg tattcatcag gatccatgta gaattgaagg
      241 ctgaggcaca cacaaagatt attttagcct tatattcata agtctgatta ttgatcgcat
      301 cgagcgtctc gaccccggtt gcctttttgc tgtctttgtc ataaagcagc ctggtaacga
      361 ttttatttac ctccaaactg agattaccgg tagcttttgc tgcaggtagt gtggaggatt
      421 gagtagagaa atatgctcca tatggacagc ccagccaaca tttattgcga tactgacaat
      481 tgacccggtt ctgttcaggc ataggcttgg tgatgttggc actccgccca ataaacatat
      541 gccgtgagcc cttatacttt tctttgagat tgcgggcaag atccttctcc acgatattca
      601 tctccatggc gggcaggaat tcactatccg gcaatacttc cagtgcttca taagagcctt
      661 gaatacctgc aaaacgctcc acataactat accatggcgc gagatcttca taccggatcg
      721 gccagtcgac agcaatgcct tccttgccat tcgcctcaaa atcccattta ttccagcgat
      781 aactttgtcg tccccacaac agagaacggc cgcccagatg atatccacga taccaatcaa
      841 aacgtttatc ctcaatatat gggctttcga ggtcattgac ccacatatcc agaacttttt
      901 cattcaacgg gtaatcacgc ttaagcaccg gatgtgtatt gatcatctcc ctggtgcgct
      961 cgccacgatg ctcaaacacc cagggatcct tgttcgcatt gacatagtcc ttgatgtgct
     1021 caatgttacg ccctctttca agcatcaaca ctttgagccc tttctcggcc agctccttag
     1081 ctgcccatcc gccagctatt ccagagccga tcactatcgc atcataaata ttatccgcca
     1141 ttcgccattt gatttaattt cccaaattta gtataaaaat tcttctgcca gacaaaaaat
     1201 aatgatatcg atgtccacta ttcttaaaat aaaaccacga attggcctta ttgccaaaca
     1261 aacgccccat cttccaaaac ctttctatca aaaacaatgt cagaaaatag ccaattcaat
     1321 tgatatttct catcttacct tgcaactcaa tgacatggtc tgagctaagt ttaaattcac
     1381 cataattggt ataaattttt tgattgtcgt aagatctgac tatctctgac cggattgctt
     1441 ccgcgcgatt tatcctaaca aaataatttt tttgattgtc aggaaggatg gattcaaggc
     1501 aattggtcaa agaggtcctt attaaaaaag tctgagtata tccaaaagct tttatctcca
     1561 cataattttt aatggccctt atcaggtaca cttccgacca aaatatttta aaagtcttgt
     1621 ttccaatctt ggtaaagaaa tactgtgccg gcgactctgg tgaatctgaa tggaccggag
     1681 tctggttgga gttgaagttc tgcatagcaa tctgaattgt actaaagatg gtcttgtcat
     1741 tgaccggctt gaccagataa gcggacggtc ccagcttaga agcactctcg acggtttcct
     1801 tatcactata tgccgttatg aaaataaaag gtgtaggttt tactttcttc aatctagagg
     1861 caagatcaag ccctgtcctt ccttcggcat catccagatt gatatccata attgcaagat
     1921 cgatttcgcc tttctcaatg acttctattg catcatcaaa agtattacat accgtcacac
     1981 actcatatcc aagatccacc agactctgac gaatattctc tgcaataatc acctcatctt
     2041 ctactaacaa caactgaagg ttattcatat cctacccatt tttattttat aatgataaag
     2101 acctctatgt cctttaaaca attctgcctt gatttgtttc atcaatagtt gcacaatatt
     2161 tgttcccagc ccggaagatg gctctgtttc tgccttttga tccccattgt cataatactc
     2221 gattactacc tggtcatttt cttgaaaaac attcaaagtt atttccaaat atttgtcacc
     2281 caggattgca tgtttaatgc tgttggaaat ccactcattg ataattaagg ctatcggaaa
     2341 attatactta tgtggtattt ctactttttc aaaattaatt tccgaaataa tatgtgtgct
     2401 caatgaaaaa cagttgatga tcagctgaat aagcctgaaa ataaagtctt tgaaatcgat
     2461 cttaccatcc aaattcatca tctcctcatg cataatggca atactttcta ttcttagtct
     2521 ggcatctctg aggttgagtt ttgttacctt atcatccgtc tttctttcct gcatctgcaa
     2581 caggctaaaa ataagatgca ggttattctt gatcctatgg tggagttcct tgtaaagtat
     2641 ggccgcattg ttattggcaa actccagatc ctttgaaagt ttataatact tggtcgaacc
     2701 tctccttaaa aaaataatga tgtaagaaga cgcaatggca aacaatacca gccaacatat
     2761 gattatcacc cacagctgat ctgttttttt ctcaatattt cttgtatgat cgagtgatcg
     2821 ctccgcaaac tcaatcttct gtttcgccag tttgtcatac cttgcattct catgtaagag
     2881 caagttggtc aagatagtct ggtaatgaaa ggctttttca aacctccccg acctctccat
     2941 tagactagtg agccttcctg catagaaaag actatcctgc ataataccga gtgtactgta
     3001 caacttatcc agtgaatcca acggttgaag attatcttct tttgcaaaaa aaccggcttc
     3061 actgtgtaaa agatagtact tcatcggatc agggacagcc aggtccggtt gcttcaattt
     3121 caatatttca ataccttgaa gcgctttttt gaccagtatt gtgtcgtgac acatcaatgc
     3181 caccatgccc aagtcaaggt aaattccgga cagggttgtc atatcaagac tgctatttga
     3241 aattttctcc aataattgct cgaggatagt caggactttg cttgattcac tcctttccaa
     3301 gagtgattta cataacaata actttgattc ataataatga ttttcataat ctttatcctc
     3361 ctcaaacagt ttcccggctt cttcaatata ttcgatagcc atgtcaggtt gtgttcccca
     3421 gtcataatac atcctaccca gagaaagata attggtggcc agtaagcttg aatcagccag
     3481 gccatgagca atcaaggcag ctgaatcata atatacctta ctgtcaataa tggctgttgt
     3541 tgatgattta agcttactca ggctatctcc catgctttga tagtaccgga acctcagtcg
     3601 cagacattcc ggatcggaaa tacacacgcc attatcgtcc cgacatgaag aaatagtcag
     3661 gacaaacaaa ataccaagta atggcagata aatattcgag cgcacaggat tgtaataata
     3721 tatcaaaggc aattattacg tttcttacaa aaattaaaca caaatttata aaaactcaaa
     3781 tgctcattat atttttttaa ataaattatc tttatatttt attgggtttt ctaattccca
     3841 tcaatagcat ccggcgcatg gcccaaactg tttatattat tgcaatcccc cttctcaggg
     3901 ttaatttgat aaagaatccg ctgactgcaa aaaaaattca atctcaacca tcctattatc
     3961 accgattaac atgcaattcc ccttatcccg acctttatta acccaaatgt cacatcagta
     4021 cagatttgac ttgccttacc ttcaggaagc ggatattcaa cgcacagaaa atctagtcgt
     4081 ttgttttact gcggatccct agctggtact gcttcggtgt tataccttca taatgtttaa
     4141 aagcacggat aaagtagtta ctatcttcaa aaccggcttc gtaacagaca tttttgatat
     4201 aggtattctt gtcactaagg aactgaactg tttagctagt ctgatccgct cagtcataat
     4261 aaattccctt gggcttatac caagttcagt cttgaacaat ctgtacaaag cggaattgct
     4321 catacctgcc tttttaagga taaatttaag gtcaagcctg gtgttcaggt tatcccgaat
     4381 aagtgtaatt acttgctgga gctgagggct cagcttatta atatctcccg cttcggcgct
     4441 ttgcaaactc tggctttgga caatcttgac caatagttcc tgaatactaa gatctgcaag
     4501 aacatcttta aaaaaggtat cattttgaca aattgaaata atcttaccca ggatggtagc
     4561 aatctcttca tcattttgaa aaatgaaatt atcaaaattg agtttccaaa aattatcatt
     4621 gccttcccgg gggtatcttt cattaagaaa atccagggtc tttgctactt tatgattatc
     4681 gatagccaat gtcagacatt gcgtaggatt cttcaaatta gtatccggaa aatctatgtt
     4741 taccgctgca ttaggaggta caatagctgt ctgtccgggc ttaaaaaaaa tatcatcctt
     4801 ttccatgcga tgaaggacct tcttccccct tagcatactt gctaccataa attggtcaaa
     4861 cttaaagtaa actgacttgg ccggtatata agtctcatac aaagagagtt cataattgtc
     4921 caaagtatag caggtcctgt tttcaaccaa ggttttcaaa gagccttcat gtgttagtgg
     4981 aggagctgga ggaatgtggg ttagaactgg catggtataa agaggttagt ggtctatata
     5041 taagagatat aatcaatcat gcaaaataaa ataaaaaatc gcaacaatcc tacattgcca
     5101 tgaaataatt ttttaaaccg gacaagcagc catacaaagc ccaaacatat tcaaaatcca
     5161 caataaataa taccacgcaa ttcagcaggc tgaatattaa gaaaaaagct ttatcatacc
     5221 attaattacg ccttcgaaga tagaaaatct gaagcgtcga tatcttcctt tgactacact
     5281 tattgcttaa aaaaaccata ccattgtaca cctgtccaaa gtaaagcaac aatttgaatc
     5341 aaacctacac ctacacaata tatcctaccg attttcaaca ataaggaaaa ttgaaggata
     5401 caaaattgtg caaatgtgct actttttgat tgatttatgc tatttttgac ttgccggcca
     5461 cattaacttt gctttaactt aaggttgaaa gataattttc ttttaacaat tgacatcagc
     5521 tatattattt cgttactgtg tcaaagttaa aataaaataa tttttagccg gaactgatct
     5581 tcggatcaga agttgaagga aataatggta atttaacgaa attatcagaa tcgaccggga
     5641 atcttgatcg ttagaaagat tggcgcatag ataatcgtaa tcatttaatt ctattcgctc
     5701 atttatatca agcctatcgg taatatttga cataatcatt cattcgtcct gtgtatcaat
     5761 aaaatatggg aaaatactac ctctactatg cttgattggt atttattaag acgaaatgaa
     5821 gaagtagaat ggggcatttc gcttttaata aactcatttt attaaggtgg aatgccctgt
     5881 tcaggctttt atgatgaaag ttgaatacat cttttcaatg caatagaaaa aaaatacctg
     5941 ctgtcaggta tgtaagataa ataagtgtgt gaatcgtttt ataaaaccga tagggtacgt
     6001 gttctgtcgg ttttttttat ttacccacga ttctgttctt tctgacatcg atctgtggtc
     6061 aattgtacgc aaaggacaac aatatccctc ccttttgtcc tgaagacata aatttgtatt
     6121 tttgtaagtc aatccgaaat aattatgtat attttcaata atttcatcgc ttctttgtta
     6181 ttgttggttc ctgcttggtt tgccaacatt tctgaagact acacaaagcc caatcttcaa
     6241 aaaacatata agatactatt tgtagggaat agccttagcg ggaccaatga cctgccggca
     6301 cttttcgaga ataaagctaa aagtaaggac attgatgtag aagttcactc gctcatttcc
     6361 cccgcacact ctctggaaga ccatctgctg ggcaacagga tccaattaac catccggaat
     6421 ggccattttg actatgtcat catacaacaa ggaccgtcac caggctcatc aggaagggat
     6481 gtcctgataa aggatggtgg taaaatacaa agaatgtgca aacaagccgg ttccaaaatg
     6541 gcttatttga tggtatggcc gtccaaaaaa aactacaatc gatttgacac agtgatttcg
     6601 tcttatacac aggccgccaa aaagaacaat gcgatactga tcccggcagg acaggtttgg
     6661 aaggattaca tcgatgctac caatgattat agttattacg gaagtgatgg attcaatcct
     6721 tccttcaagg gtagtgaggt ggcagccgag gtgattttca atacgttatt cgggaaatag
     6781 agcctgctcg cccacatcaa tgttcttccc tcacaaactg tacgttgcgc ttcatacctt
     6841 ccagacccgt tcgcttgatt ggtgactgga agaaaatgga ttcaaaacgc tccggggtca
     6901 tttgttgcca atcttccggc atgatctccg ctatacccga taccggggta aaatccgcct
     6961 cccgggtagg agtcgagaat ttattccatg ggcacacctg ctgacaaata tcgcaaccga
     7021 acacccaatc ctccattttc cctttaaatt catccgggag cgcctcatcc ttgagctcaa
     7081 tagtcaaata ggaaatacac ctcgaagcat ccagc
//
LOCUS       DAOQQN010000144         7098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4337_length_7098_cov_5.790146, whole genome shotgun sequence.
ACCESSION   DAOQQN010000144 DAOQQN010000000
VERSION     DAOQQN010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7098
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4337_length_7098_cov_5.790146"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PLG24_06685"
     CDS             complement(<1..533)
                     /locus_tag="PLG24_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24610.1"
                     /translation="MKICVFLLQFFFIFLSFSIQVNAQFDKLKKSATESYKTISNSNI
                     GKEISNRSKNTTAPEKQDNKNTSSNQSSVTNYYIRPDGRGKDGTKENPAKDIAAIAMQ
                     LKPGDVVHVAAGVYKSKADQSSDVFAVPISIIGGYSPDFSKRDPWGQYQTVFSGTNDI
                     SKGLTTERIGILTDKGY"
     gene            1227..2255
                     /locus_tag="PLG24_06690"
     CDS             1227..2255
                     /locus_tag="PLG24_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ24611.1"
                     /translation="MKKNRRDFIKATGLLGAASFLPLNNPLSMLNKADNFGDCVLIPS
                     ETAGPFPLDLTDNTYYFRQDVREDRTGTLLKLKLKIIGLQNCLPMQNVRVNIWHCDRA
                     GLYSGYYNPINIGSTPETANMKWLRGYQLTDANGEVEFITIFPGWYQGRTCHIHFQVH
                     VSSSYSAISQITFDVDTKQAIYAANPAEYTKGPDPTTPSKDNIFSDGYQYQVATLTPG
                     DDGGSYNAYMEIAVKGSGTTAGTGHLEKENAKNFTLGQNYPNPYIDETTIPIRLLHPS
                     SLTLDLYDLQGNKVTSIQKNNLLPGELNIPLNMKTLGLPARSYVYQVTIKNNNGTYKD
                     CKMMTLAK"
     gene            2660..5233
                     /locus_tag="PLG24_06695"
     CDS             2660..5233
                     /locus_tag="PLG24_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ24612.1"
                     /translation="MDDVLSIILKDLRFYTEDSNYQYPGNELRYRTRFDKEELVRLKW
                     DLEVINLNYTKEKQQLDLTIRCTALTKRMQKFSKVLTVELLINDLIKTVSGSLEVGAI
                     HDWEADQYLWEVLIGDKLLMRRTFVLDAKGTVSSHSNPYFDLFSTALCVTDESGTLRY
                     LNTFNSHKTAKIFIRLSFKRKFTESKNLEFNLVIFNGLTGQLVASKQFNFSYPAREGL
                     SVDNALFSCGEDKPGYWAIGSYFYYISFMGTTVSSGQFLVSTEEVEGAPMGIDGNAIK
                     TQTASNTDGLDDALKELDNLVGMTQIKKSIHESIAYLKFNKLRVEKGFSDDGELVLHS
                     IFTGNPGTGKTTLVRLLSKIYKAMGLLSKGHIIEATRADLVGEFIGQTAPKTKKVIVS
                     ARGGILFLDEIYALSRGNNNTDFGAEAVEIILKEMSDGPGDIAIIGAGYPDEVTSFLN
                     SNPGLKSRFSMHYHFEDYVPAELLQIADVALRKEEVALSSEAHDDLAFYLTELYRKRD
                     RTFGNGRLVNHIIDQAKKFMGIRLLRLSDTQEITKEAMSIIQKEDLLKVFAAEQKNKL
                     ELNINQAELTLALDRLHRLTGLDHIKKEVNDRIQLVKFYNETGKNILNQFSLHAIFTG
                     NPGTGKTTLARLLGEIYKALGLLERGHVVEVSRQHLVAGYVGQTAIKTAEVVQKAMGG
                     ILFIDEAYSLNSNGLNDFGDEAIETLLKLMEDERGKFAVIAAGYPDNMNQFVLSNPGL
                     KSRFDTFYNLPDYNPEQLLAIAIQLMAQSNLVPDVETKQFLANYFKSAYESRDKYFGN
                     ARLVRNVVETIIGNQYLRMAHLPPGERTTDSLKTVTLADVGNMKENEIPKTKGIGFKA
                     T"
     gene            5295..5729
                     /locus_tag="PLG24_06700"
     CDS             5295..5729
                     /locus_tag="PLG24_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24613.1"
                     /translation="MKYLPTLLAEHFAKGYNIKLSSPDNYTWDYGLVYPDGSNRFQYV
                     YLSEFTENEKDYLYIRSFVADFDDRFNTAELLREADFGRMCFISLKPYTKTDGTNVEG
                     IYVTCCLPVGVAQSDKEVFKDVIHEVANRADALERKFLNVDN"
     gene            6767..>7098
                     /locus_tag="PLG24_06705"
     CDS             6767..>7098
                     /locus_tag="PLG24_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24614.1"
                     /translation="MMYADVLGRQRNFFRSGETKQIDFRKEQLRKLRDVLQGHEERFY
                     EAIQQDLGKSAFETFTTEISFIYNEIDYFIKNLSSLAKPRKVGTNIINQLGSSKIYWE
                     PFGNTLAIG"
BASE COUNT         2161 a         1325 c         1470 g         2142 t
ORIGIN      
        1 ttatatccct tgtctgtaag tattcctatt ctttcggtag taagcccttt actgatatcg
       61 ttagtcccgc taaataccgt ttggtattgc ccccagggat cccgtttact aaagtcagga
      121 ctgtaacctc caattataga aattggtact gcaaagacat ctgaactctg atccgcttta
      181 ctcttataga caccagctgc aacgtgaacc acatcgccag gctttaattg catggcaatt
      241 gctgcaatat ctttagccgg attctctttg gtaccatctt ttcctctacc atcaggacga
      301 atatagtaat tggtaacact gctttggttc gatgaagtat ttttgttgtc ttgcttttct
      361 ggagcagttg tgttttttga tctattagaa atttcttttc caatattgct gttcgatatg
      421 gttttatagg attcggttgc tgattttttc agtttatcaa attgggcatt cacctggatt
      481 gaaaaagaaa gaaatataaa aaagaattgg aggagaaaaa cacagatttt catgatattt
      541 attgttttag tttaaatttg ataggccaaa gataccgtcc tgaaaaaggg gaataataat
      601 cagatgaagt caacacggaa aaatgttgag tgaagattta aaatgtattt agtgaccttt
      661 ttaaaagcat tttatcttca ttattcccat atcctccact aggagatgga agcacagtta
      721 atggccaaaa aatgtggttt tttattacct tgttacctat cgtcagaatc aatgatagtg
      781 cttcctgtag attgtaccgg caaataacgt attacatgag gtgataggat ttttcaaact
      841 aaagatcaga tatcaacttt tccatattaa atcgatattt caataaaact gagcatgtca
      901 ggaagggtta taacctttat tatataaaga cggacggtaa ctcctgtatt cccaatctgc
      961 aataccgcca ttactccttc ccgacacatt taatataaat gcaactttta atagggtagg
     1021 agaccgcatt atggatagat tttagccaaa tatgttcggt gaaaatgttt ctacgtacta
     1081 tagggtaatc ccaacatttt caattccttt gttctaaaac atgatacagg tatattcatg
     1141 aaataatgta gttggcagtt gcgttgaaag aattattaag tcataaagca tattgtacta
     1201 tatttatgct aaattgaaac atagaaatga aaaagaatcg cagagacttt attaaagcaa
     1261 ctggtctgtt gggtgcagcc tcgttccttc ctctcaacaa tccactcagt atgctgaata
     1321 aggcagataa ctttggtgat tgtgtattga tacccagtga aacggcgggg ccatttccgt
     1381 tggatttgac tgataatacc tactatttca gacaggatgt acgtgaagac cgtaccggta
     1441 ccctccttaa gttaaagttg aaaatcattg gcctgcaaaa ttgcctgcct atgcaaaatg
     1501 tccgcgtcaa catctggcat tgtgataggg caggccttta ttccggatac tataacccaa
     1561 taaacatagg ttccactcct gaaacggcca atatgaaatg gcttagaggc tatcagctta
     1621 ccgatgctaa tggcgaagta gagtttataa ctattttccc gggctggtat cagggtcgta
     1681 catgccatat acattttcag gtacatgtta gttcttctta ctctgctata tcacagatca
     1741 ctttcgatgt agacactaag caggcaatat atgcggctaa tccggcagaa tatactaaag
     1801 gccctgatcc gactactcca tccaaggaca acatcttttc agatggatac caatatcagg
     1861 ttgctacgct gacacccggc gatgatggag gtagttacaa tgcatatatg gagatagctg
     1921 tcaagggtag tggcacgact gcaggtaccg gacacctgga aaaagaaaat gccaagaatt
     1981 ttaccttggg tcagaattac cccaatccat atatagatga aaccaccatc cccatccgct
     2041 tattgcatcc ttccagcctg actctcgatt tgtatgatct tcagggtaat aaggttacat
     2101 ccattcaaaa aaacaatctg ctcccgggtg aattgaatat tcctttaaat atgaagactt
     2161 tgggcctccc ggccagaagt tatgtttatc aggtgaccat taaaaataat aatggcacct
     2221 ataaagattg taaaatgatg accttggcaa agtaataaaa ggatgcctgg ccttttgggg
     2281 cttgttttta aataataaat gctgtcaatc cgccggcatc gtatagaaca gttcatacag
     2341 catattaatt tttctgcccg gcataaccag gtaaaattgc tttattgact agttgcttct
     2401 cgcggtatta atagtttttc tggaggaggg ccttgtcatt acagaataat agccatatag
     2461 aaacattatt ccaaagaaag gttggagttg cgctgccatt ggtataaaaa gctctaaatc
     2521 aggaaaattt tgccctgacc tcaataactt tagttttgtt ttacttggtg aaaggagaga
     2581 aaactttttt attaaaggtt atactttatt cttattttgc aatcatattc aaaccactta
     2641 atcaggaaag tcagcctata tggacgatgt tttgtctatt attttgaaag atcttcgttt
     2701 ttataccgaa gatagcaatt atcaatatcc gggcaatgaa ctcagatata gaaccagatt
     2761 tgataaggag gaattggtcc gtctcaagtg ggatctggag gtaatcaatc tcaattacac
     2821 gaaagaaaag cagcagttgg accttaccat cagatgtacc gctttgacga agcgaatgca
     2881 gaaattttcc aaagttctga cggtcgagtt acttataaat gatcttatta agaccgtatc
     2941 gggctctctg gaagtcggtg ccatccatga ctgggaagct gaccaatatc tttgggaagt
     3001 attgataggc gataaattgt taatgagacg aaccttcgtc ctggatgcta aaggaacggt
     3061 ctcatctcac agcaatcctt acttcgactt gttttccacc gctctatgtg taacggatga
     3121 atccggaacc cttcgatatt tgaatacatt taacagccac aagacagcca aaatttttat
     3181 cagactatcc tttaaacgga agtttacaga aagcaagaat ctggaattca atcttgtcat
     3241 attcaatggc ctgaccggtc agcttgtagc atcgaagcag tttaattttt cctatcctgc
     3301 cagggaaggt ctgtcggttg ataatgctct ttttagttgt ggtgaagata aaccgggcta
     3361 ttgggctatt ggtagttatt tttactatat ttcatttatg ggaacgactg tttcttcagg
     3421 ccaatttttg gtgtctactg aagaggttga aggtgccccg atgggtatcg atggtaatgc
     3481 catcaagact cagacagctt caaatacgga tggactggac gatgcattga aagaactgga
     3541 taaccttgtc ggtatgacac agattaagaa atccatccat gagagtatag cttacttaaa
     3601 gttcaataaa ttgcgtgtag aaaaaggttt ctccgacgat ggagagcttg tgcttcacag
     3661 tatatttacc ggcaatccgg gcacaggaaa gacaacctta gtccgccttc ttagcaaaat
     3721 ctataaggcc atgggcttgc taagcaaagg ccacatcatt gaagcgacac gagccgacct
     3781 ggtaggggaa tttatagggc agacagcacc aaagacgaag aaagttattg taagtgcacg
     3841 gggaggcatt ttatttctgg atgaaatcta tgccctgagt cgcggaaaca acaatacaga
     3901 ttttggcgct gaagcagtgg aaattattct taaagaaatg agcgacggac ccggcgatat
     3961 tgctattata ggggcgggtt atccggacga agtaacgtct tttctcaatt ctaatccggg
     4021 cctgaagtcc agattttcca tgcattatca ttttgaagat tatgtaccgg cggaactctt
     4081 acaaatagcg gatgtcgctc tgagaaagga agaagtggca ctttcttctg aggcacacga
     4141 tgacctggct ttctatctga cggaactata ccgtaaaaga gatcgcacct ttggcaatgg
     4201 tcggctggtc aatcatatca tcgatcaggc aaaaaaattt atgggcatcc gccttctccg
     4261 gctttctgac acacaagaaa ttaccaaaga agctatgagc attattcaaa aagaagattt
     4321 gcttaaagtt tttgccgcag aacaaaagaa caagctggag ctgaacatca atcaggcaga
     4381 actcacctta gcattggatc gactccacag gctcacaggc ctggaccata tcaagaaaga
     4441 agtaaacgac agaatacagc tcgttaagtt ttataatgaa acgggtaaaa atatcttaaa
     4501 ccaattttca ttgcacgcga tattcacagg caatcccggc accggcaaaa ccacccttgc
     4561 ccgcctcctt ggagaaatct ataaggccct gggtttactg gagcgtggtc atgttgtgga
     4621 agtaagcagg caacatttgg tagccggtta tgtcggccag acagctatta aaactgcaga
     4681 agtcgtgcag aaggcgatgg gcggcatttt gtttattgat gaggcttatt cgctgaatag
     4741 caatggcctg aatgactttg gtgacgaagc aatagaaact ctgctaaagt tgatggagga
     4801 cgaacgtggt aaatttgccg taattgctgc aggatatccc gacaatatga accagttcgt
     4861 actttccaat cccgggctga aatcacgctt tgatacgttt tataacctgc cggactataa
     4921 tccggaacaa cttttggcca tagctataca attgatggcc caaagcaatc tggtacctga
     4981 tgtggagact aagcagtttt tggctaatta tttcaaatct gcttatgaat ccagagataa
     5041 atattttggc aatgcccgac tggtgaggaa tgttgttgaa accattattg ggaatcaata
     5101 tcttcgtatg gcacatttac cacccgggga aaggactacc gactccctca aaaccgttac
     5161 cttggctgat gttggcaaca tgaaggaaaa tgagataccg aaaacaaagg gtattggctt
     5221 taaggcaact tagattgcca taataaattg cccgaatgaa tattacaaat taataaccat
     5281 ttaaattaaa cacaatgaag tacttaccga ctctcctggc tgaacatttt gcaaaggggt
     5341 ataatatcaa gctttcttct cccgacaatt atacctggga ttatggcctg gtctatcccg
     5401 atggatctaa tcgttttcaa tatgtttatt tgagtgaatt cactgaaaat gaaaaagatt
     5461 acctctatat tcgaagtttt gtggctgatt ttgatgatag gtttaacacg gcagagctgt
     5521 tgagagaggc tgacttcggt cgcatgtgct tcatatcttt aaagccatat accaaaactg
     5581 atggcacaaa tgttgaaggg atctatgtta cctgttgctt gcctgtagga gtagcacaaa
     5641 gtgacaaaga agtctttaag gatgttatcc atgaagtggc aaaccgcgcg gatgccctgg
     5701 aaaggaaatt cctcaatgtt gataattaat tctctgataa agatagatta aattattacg
     5761 gtagacattg atactcatac atatcattct ttaatattgg tgtaacttta ggttaagtgc
     5821 cctgatttcc atgatttcgg ggtatgacct ggttgtcaca tcgtcaaatt cgacttgacg
     5881 gactttgatt tgatgtggca cctggttttg gatagtatgg gaataaggat tcaagccgat
     5941 atgaaactaa aattccaaat tgaccttttt aatctgtatt ttaaattgga atctaagctt
     6001 agtggctgga aaccggatta ttttattccc ggctatcctc ctcacgctaa gacatacctt
     6061 ccctttgttt gaaatgctga cttattgtct tgattgatgt ctttacagct acaaagattt
     6121 tcgatttatt acaactaaaa ttcacacttt attcaaatta cttcctatga agtagctttt
     6181 attaataaaa tatgagtagg gcatctgtta accttagaat aaataggtag acttttaata
     6241 aaatatacga agccactttt cacgagacca aaacagattc atgatcttta tttgtcaaat
     6301 atttttactt agccggctta ttcgctgcaa tccgcacacc ttgtatttcc caaatgtttc
     6361 aaaagaatat tgaactggaa atggttgggc cggaaaggaa aaatcggtaa agtgggcaat
     6421 tagcctttaa tattcattgc aaaggccgaa agatatccgg aattgaagtt atgaaggtat
     6481 ctaaatgaag atttgaatca gctttctcaa aaggaaaggt gcagcatatc cattggttaa
     6541 tggcaacaat tgtttgcttg tttgtaaagt ctaactatca attggatatg cgcagtcagt
     6601 gattggggaa ggagttaggt tggtggagta tcaaaggacg aatggaatca gctatttggc
     6661 aatttgtttt ttagatttgc cttttcagac tttttgtaaa caaattttca gatttatact
     6721 ttatttgtaa gtctttagta gattttatat aaataaatag tttaatatga tgtatgccga
     6781 tgttctcgga cgacaacgta attttttccg gtccggagag acaaaacaga ttgattttcg
     6841 aaaggaacag ttgcgaaaat tgcgggatgt tctgcaaggc catgaagaaa ggttttatga
     6901 agccattcag caggaccttg gtaagtcggc atttgaaacc ttcactacgg agatttcttt
     6961 tatatacaat gagatcgact atttcataaa gaacctgagt tctttggcta agccccggaa
     7021 agtaggaacc aatatcatca accaattggg cagcagtaag atttattggg agccatttgg
     7081 caataccttg gccattgg
//
LOCUS       DAOQQN010000145         7080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4357_length_7080_cov_4.989039, whole genome shotgun sequence.
ACCESSION   DAOQQN010000145 DAOQQN010000000
VERSION     DAOQQN010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7080
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4357_length_7080_cov_4.989039"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2383
                     /locus_tag="PLG24_06710"
     CDS             <1..2383
                     /locus_tag="PLG24_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ24615.1"
                     /translation="DNPNLLNTLYTPGPNDILNGSVVLILTTLDPDGAGPCNPVSDAI
                     AITITELIIKINPVPDVCIDGKEITLSGTPLGGIFSGPGVTGNIFDPAKAGVGNHIIT
                     YTIEGNCPGSVDFNINVLPLPGVTINPISNLCVGDPAITLTANPPGGVFTGQNVSGDR
                     FDPVQPGVYTITYTYTNNAGCVNSSSVDIQVADCGCADPASADAGKDITICEGETVTL
                     TGTITNAPGAVWSSNGTGLFSDANALSTTYEPSVTDIKKGSVKITLTTLDPDGNGPCH
                     PYSDQLTITIIPLPKVSIITPPNEICQDGAILTLIATPQGGTWSGNGVSNDKFDPANA
                     GIGDHKLTYTYINGTCKGEDSFTLTVKDCGCNTLVNVNAGPDINICDLQPVTLKGSVN
                     GNFPVVWSTNGTGTFSDRYSAVTQYTPTEADREKQIISFTITSPDPDDDGPCEAKTDM
                     MSLSITTLMDVNLKIDDTDCGHDRGSVTVLPPASNRFIFSVDNGNTFTDLPVFGDLAP
                     GNYSLIYKDPISGCQSSKNFTINPPPLVAAQWRLITKACAPVESNFIDIVSTQNLTLP
                     LDVYLDDKYTLTTATLPVKIDHLEIGKHTLKIKDANGCIVNDNFEITESSTIDVDILP
                     VYVVDEGESAVLIPQINGPYTSIVWTPADYLSCTQCPTPTTTPDREITYLVTVTNQQG
                     CEDSIDVRIIIRKKIDVFVPNIFTPNGDGLNDFVTIFTDSHIKIIDNLKIFSRWGELV
                     FERSNFLPNIEAEGWDGNFRGQAMNPAVFAWVATINIPGEGIRIFKGDVTLIR"
     gene            complement(2407..2796)
                     /locus_tag="PLG24_06715"
     CDS             complement(2407..2796)
                     /locus_tag="PLG24_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24616.1"
                     /translation="METTTESKMCLCCSKKINGRADKKFCDDFCRNTYNNRLKGNINN
                     VIRNVNGALTKNRRILESLLPESEMHKFVTKEKLVKLGYRMKYHTHVVNNQKGQTYFF
                     CYDHGYKEADGGNVMIVRSYIPERPSV"
     gene            complement(3146..7069)
                     /gene="rpoC"
                     /locus_tag="PLG24_06720"
     CDS             complement(3146..7069)
                     /gene="rpoC"
                     /locus_tag="PLG24_06720"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HMZ24617.1"
                     /translation="MGYLLGVSSKKLESIIYYERYVVVQPGVKESSGVNRLDLLAEEE
                     YFEILDSLPKENQLLDDTHPDKFIAKMGAEAVRDLLMRIDLDQLSYELRHLASTETSQ
                     QRKSEALKRLRVVESFREGMETTENRPDWAVIQYLPVIPPELRPLVPLDGGRFASSDL
                     NDLYRRVIIRNNRLKRLLEIKAPEVILRNEKRMLQESIDSLFDNSRKSNAVKAEGGRA
                     LKSLSDILKGKQGRFRQNLLGKRVDYSGRSVIVVGPTLKLHECGIPKGMASELFKPFV
                     IRKLIERGIVKTVKSAKKLVERKEPVIWEILENILKGHPVLLNRAPTLHRLSIQAFQP
                     KLIEGKAIQLHPLVCSAFNADFDGDQMAVHVPLSQASIIEAQLLMLSSHNMLNPQDGS
                     PVTLPSQDMVLGLYYLTKPKVSTPESPVRGEGMKFYSSEEVLIALNEGKLDLHAQIKV
                     RERVEENDSMVLKLIDTTVGRVLFNKAVPANVPFVNELLTKKNLKKVINDIIKRTNFA
                     VTADFLDGIKEMGYFWAFKGGLSFNLGDLIIPKVKVTAISSAQNQVDEIMEHYNMGLI
                     TNNERYNQTIDKWTFADNMITNSLMKELSNDKQGFNSVYMMLDSGARGSKQQIKQLCG
                     LRGLMAKPRKSGDTGGAIIENPILSSFIEGLSVLEYFISTHGARKGLADTALKTADAG
                     YLTRRLVDVAQDVVIVEEDCDTLRGIEISALKDNEKIIEPLSNRIVGRFSLHDILHPD
                     TDDLILEAGEYIDETVAEKIEEVGIESVTIRSVLTCETKRGVCTKCYGKNLATGRIAE
                     IGDAVGIISAQSIGEPGTQLTLRTFHTGGTASVSKSDSEIRAKYDSKLEFDGIRTSVQ
                     DDEEGSNAVVVISRTGEIKNVDIKTGKLLHTEHVPYGAKLRFKDGDKVTKGDLICDWD
                     PFNALIISEFAGKAQFESIEEGVTFRVERDDQTGFAEKVIIESKNKRKIPMIKIVDKK
                     GDELRNYSIPVGSYLQISDGDKIKAGQVIVKIPRKLGKISDITGGLPRVTELFEARNP
                     SNPAIVAEIDGVVKFGMIKRGNREVSISSKDGQERKYLIGLSKHILVQDGDFVRAGTP
                     LSDGAIAPKDILDIKGPFAVQSYLVNGVQEVYRSQGIAINDKHIEVIIRQMMRRVEII
                     EPGDTNFLEGEAVEKYYFLEQNDFVFDKKVITDSGDSNKFKPGQLVTIRHIREENSIL
                     KRDDLKLVEYRDAAAATSMPLLLGITKSSLGTESWISAASFQETTKVLSTAAIAAKKD
                     MLAGLKENVIIGKKIPAGTGVRKFNDLLVDVDGNGNANNEAEVMY"
BASE COUNT         1978 a         1615 c         1330 g         2157 t
ORIGIN      
        1 tgacaatcca aatcttctaa ataccttata tacaccaggg ccaaatgata tccttaatgg
       61 ttcagtcgta ttgatcttaa ccactttgga tcctgatgga gcaggccctt gtaatccagt
      121 cagtgacgcc atcgcaataa caataaccga attgattatt aaaatcaatc ctgtccctga
      181 tgtttgtatt gatggcaaag aaataacctt aagtggtact cctttggggg gaatattctc
      241 cggtcccgga gtaaccggta atatattcga ccctgctaag gcaggagtag gaaaccacat
      301 tataacctat acaatcgaag gtaattgtcc cggcagcgta gatttcaata tcaatgtact
      361 gcccttgcct ggcgtaacta ttaatccaat atctaatctt tgtgttgggg atccggccat
      421 taccctgaca gctaatcctc caggaggagt attcacggga caaaatgtaa gtggtgatag
      481 atttgatccg gttcaacccg gagtatatac cattacctac acttatacca acaatgccgg
      541 atgtgtcaac tcctcttcag ttgatattca ggtggcagat tgtggttgtg ctgacccggc
      601 atctgcggat gcaggaaagg atattacgat ttgtgaaggt gaaacagtta ccctgacagg
      661 taccatcact aacgcacccg gggcagtctg gagtagtaat ggcaccggat tattctcgga
      721 tgcaaatgca ctttctacaa cctacgagcc tagtgtaaca gatattaaga aaggttctgt
      781 taaaatcact ctgactaccc tggatcctga tggtaatggc ccgtgccatc catattctga
      841 ccaacttacc attactatca tacctttacc taaagtatcc attattacac ctcctaatga
      901 gatttgccag gatggagcaa tattaacatt aattgcgacc cctcaaggcg ggacatggag
      961 tggaaatggc gtctcgaatg acaaatttga cccggccaac gcaggcattg gcgatcataa
     1021 gcttacatat acttatatca acggcacttg taaaggagaa gattcattta ctttaactgt
     1081 gaaggattgc ggttgtaata ctctggtgaa tgtgaatgcc ggccctgata tcaatatatg
     1141 tgatttacaa ccagttacac tgaaaggttc agtgaatggc aattttccgg tagtatggtc
     1201 cacaaatggc acaggtacat tctctgatag atatagcgct gtgactcaat acacccccac
     1261 agaggctgat agagagaaac aaatcatttc gtttaccatt acaagccctg atccggatga
     1321 cgacgggcct tgtgaagcca aaactgacat gatgtcctta tcgatcacta ccttgatgga
     1381 cgtaaatcta aaaatcgatg atacagactg tggtcacgat aggggcagtg taacggtact
     1441 tccacctgcg tccaacaggt ttatctttag tgtagacaat ggaaatacat ttactgatct
     1501 gccagtcttc ggagatctcg ctccgggtaa ttattctcta atctataaag accctatctc
     1561 cggttgtcaa tccagcaaaa attttaccat caacccgccg ccgttggtag cagctcaatg
     1621 gcgccttatt accaaagctt gcgcaccggt tgaatccaat tttatagata tagtttcgac
     1681 tcagaattta actctgcctc tagatgtata tttagatgat aaatatactc tcacaacagc
     1741 aaccctaccg gtaaagatag accatctgga aataggtaaa catacgctaa agataaagga
     1801 tgccaacggt tgtatagtca atgataattt cgagattact gaatcttcca ccattgatgt
     1861 agatattcta cctgtctatg ttgtggatga aggtgaaagt gccgtgctga tacctcagat
     1921 caacggccct tatacgtcaa ttgtatggac acctgcagat tatctttcct gtacacaatg
     1981 tccgacaccg actacaactc ctgataggga aatcacatat ctggtcacgg ttaccaatca
     2041 acaaggttgt gaggattcca ttgacgtacg catcattatt cgtaagaaaa ttgatgtctt
     2101 cgtaccaaat atcttcacac caaatggaga cggacttaat gattttgtaa cgattttcac
     2161 tgattcccac attaagatca ttgacaatct gaagatcttc agcagatggg gtgaattggt
     2221 gtttgaacga tccaatttct tacctaacat tgaggctgaa ggttgggacg ggaacttcag
     2281 gggtcaggct atgaaccccg cagtattcgc ctgggtagca acaatcaata taccgggtga
     2341 gggaatacga atctttaaag gagatgtaac tttgataagg taaaagacta tcggaatccc
     2401 agatacttac acacttggtc tttccggtat ataacttctg acaatcatga cgttgccacc
     2461 atccgcttcc ttatatccat gatcataaca gaagaaatac gtctggcctt tctggttatt
     2521 taccacatgg gtatggtatt tcattctata acctaatttt accaattttt cctttgtgac
     2581 aaacttatgc atttcagatt ccggtaaaag tgattccagg attcgtctgt ttttagtcaa
     2641 agcgccatta acattcctga taacattgtt gatattgcct tttaaccgat tgttgtaggt
     2701 gtttcggcaa aaatcatcac aaaacttttt gtcagcccta ccattgattt ttttagagca
     2761 gcaaagacac atcttgcttt cggttgttgt ttccataata ataaatattt tgggttggtg
     2821 ataaaataag aaagcttgag aggaattaac tttcaagttt tcattttgca aatataatca
     2881 ttgccttgtg atattccaaa tgtatttgta aatatttaca atcggaagga agaataaata
     2941 ccgaaggtgc attcgttaca gactaatttc attgatctaa acaactgtat cctaggcgtt
     3001 ataccaaaaa tttatggact attatataaa tcatcttggt ataacaactt tgaaaataaa
     3061 aaaaaccttg gaacttttgc aatcggcaat aaaaaaggcc gcttaacatt attaagcgac
     3121 cttttcaggt gatttaacct gactatcaat acattacttc cgcctcatta ttggcatttc
     3181 cattgccatc cacatcgaca agaaggtcat taaattttcg gacaccggta cctgccggaa
     3241 ttttcttacc gataatgaca ttttctttca agcctgccag catatctttc ttggctgcta
     3301 ttgccgcagt actcaaaacc ttggtagttt cctggaatga ggcagcagaa atccagcttt
     3361 ctgttcctaa tgaagacttg gtaattccca gaagtaaagg catagaagtt gccgctgcgg
     3421 catctctgta ttcgaccaat ttcaaatcgt ctcgtttcag aatagagttt tcttctcgaa
     3481 tatgacgaat ggtaaccaac tgcccgggtt tgaacttgtt actatctcct gaatcagtaa
     3541 tgactttctt atcgaaaaca aaatcatttt gttccaggaa gtagtatttt tcaacagctt
     3601 ctccttccag gaagttagta tctcccggtt cgatgatttc aactcttctc atcatctgtc
     3661 ggattatcac ctcaatgtgt ttgtcattga tagcaatacc ctgtgaacga tatacttctt
     3721 gtacaccatt taccaaataa gattgaactg caaaaggtcc cttgatatcc aggatatctt
     3781 tgggtgcgat tgcaccatcg gaaagtggtg ttcctgcacg tacaaagtcc ccatcctgca
     3841 caagaatgtg cttggacaat ccaatcaggt actttcgctc ctgaccatcc tttgagctaa
     3901 tggacacctc acggttacct cttttgatca ttccaaattt gacgacaccg tcaatttcgg
     3961 ccacaattgc aggatttgaa ggatttcgag cttcgaacaa ttcagttact cttggaaggc
     4021 cacctgtgat atccgagatt tttcctagtt ttcgaggaat cttaacaatc acctggcctg
     4081 cctttatttt atcaccatca cttatctgta ggtaagatcc cactggaatt gagtaattcc
     4141 taagttcgtc acctttcttg tcgacgatct taatcatagg aatctttcgc ttgtttttac
     4201 tctctatgat taccttctca gcaaatcctg tctggtcatc tctttccact ctgaaggtta
     4261 ccccttcctc tattgattcg aattgcgcct ttccggcaaa ttcagagata atcaaagcat
     4321 tgaatggatc ccaatcacag attaaatcac cttttgttac tttatctcca tccttaaatc
     4381 taagcttggc tccatatggc acatgctcgg tatgcaacaa tttgcctgtc tttatatcga
     4441 cattcttaat ctctcctgtt cgtgagataa caacgactgc attactacct tcttcatcat
     4501 cttgaacaga tgttcgtata ccatcaaatt caagtttact atcatacttc gctcgtattt
     4561 cactatctga cttggaaact gaagctgtac cccctgtgtg gaaggttcgt agtgtaagct
     4621 gtgttcccgg ttcaccgatt gactgtgcag agatgatacc tacagcatca ccgatttctg
     4681 caatccttcc tgtagccagg ttcttaccat aacacttggt acatactccc cttttggttt
     4741 cgcaggtaag tacagacctg attgtaacac tttcaattcc aacttcttcg attttttcag
     4801 caacggtttc atcgatatac tctcctgctt ccaagatcaa atcatccgta tccggatgga
     4861 gtatatcatg aagagaaaat cttcctacga tcctattact tagtggctca atgatttttt
     4921 cattgtcttt caaagcagaa atttcaatac ctctcagcgt atcacaatct tcttcaacga
     4981 tgaccacatc ctgggcaaca tccaccaatc tcctggtaag gtatccggcg tccgcagtct
     5041 tcaaagctgt atccgcaaga ccttttcggg caccgtgcgt ggaaatgaag tactccaata
     5101 cggaaagccc ttcgataaag gaagaaagaa tcgggttttc gataatagct cctccggtat
     5161 cgccggattt tcttggtttt gccatcaagc ccctcaatcc acacaactgc ttaatctgtt
     5221 gcttggaacc tcgcgctcca gaatcaagca tcatatacac tgaattgaat ccctgcttat
     5281 cattactcag ctccttcatg agtgagttgg taatcatatt atcagcaaaa gtccacttat
     5341 cgattgtttg gttataacgc tcgttatttg tgatcagacc catattgtaa tgctccataa
     5401 tctcatcaac ctgattttga gcacttgaga tcgcagttac cttaactttt ggaattatca
     5461 ggtcaccaag gttaaatgat aatccaccct tgaacgccca gaaataaccc atttccttga
     5521 tgccatccaa gaagtcagcc gtaacagcaa agtttgtcct cttgatgata tcattaataa
     5581 ctttcttaag gtttttcttg gtcaacaact cattgacaaa tggtacatta gcaggaacag
     5641 ccttattaaa caacactctt ccaacggtgg tgtcgatcaa tttgagtacc atgctgtcat
     5701 tttcttcaac tctttcccta acctttatct gagcatgcag gtccagtttc ccttcattca
     5761 gtgcgataag tacttcttct gaagagtaga acttcatgcc ttcacctctt acaggagatt
     5821 caggagtaga tacctttggt ttagtcaaat aataaagtcc aagtaccatg tcctgagagg
     5881 gtaaggtaac cggcgaacca tcctgtggat tgagcatgtt gtgtgaagac agcatcagca
     5941 attgagcttc aatgatggat gcctggctca atggtacgtg tacagccatc tggtcgccgt
     6001 cgaagtcggc attaaatgca ctacatacca aaggatgcaa ttggatcgct tttccttcaa
     6061 tcaatttagg ctggaaagcc tgtatggaca atcggtggag tgtcggtgca cggttgagca
     6121 ataccggatg acctttcaat atattctcaa gaatttccca tataacaggt tcttttcttt
     6181 cgaccaattt tttagcagac tttacagtct taacgattcc ccgctcaatc aattttctga
     6241 tcacaaacgg tttgaacagc tcagaagcca ttcctttagg aataccgcac tcatgcagct
     6301 tgagagttgg gcctaccacg atcacggaac ggccggaata atccaccctt ttacctaata
     6361 agttttgacg gaatcgtccc tgcttacctt tcaggatatc agaaagtgac ttcagggctc
     6421 tgcctccttc ggccttaaca gcattggact tcctggaatt atcaaacagg ctatcaatgg
     6481 attcctgaag catcctcttc tcattccgaa ggatgacttc gggagccttg atctccagaa
     6541 ggcgcttcaa cctgttgttt ctaatgataa ctcttctgta caaatcgttc aaatctgaac
     6601 tggcaaaacg acctccatcc aatggaacca atggccttaa ctctggtgga ataaccggaa
     6661 gatactgaat tactgcccaa tccggtctgt tttcagttgt ctccattcct tccctgaagg
     6721 actctacaac acggagtctc tttaaggctt ctgactttct ttgctgtgag gtttcggtgc
     6781 tggccaggtg tcgcagttca taagatagtt ggtccagatc tattctcatc aaaaggtccc
     6841 tcactgcttc tgcacccatc ttggcgataa acttatcagg atgggtatca tccagcaatt
     6901 gattttcctt tggaagggaa tcaagtattt cgaagtattc ctcttctgca aggagatcca
     6961 atcgatttac accactgctc tcctttacac cgggttgtac cactacatat ctttcataat
     7021 aaatgatgga ctccagtttc ttggaagata ctcccaacag ataacctatt ttgttgggta
//
LOCUS       DAOQQN010000146         7079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4359_length_7079_cov_5.452164, whole genome shotgun sequence.
ACCESSION   DAOQQN010000146 DAOQQN010000000
VERSION     DAOQQN010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4359_length_7079_cov_5.452164"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            452..823
                     /locus_tag="PLG24_06725"
     CDS             452..823
                     /locus_tag="PLG24_06725"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HMZ24618.1"
                     /translation="MNEKEMPDRETLKAENTKAQMRKGVLEMCILGIISGEELYTSDI
                     INKLKENELVVVEGTVYPLLTRLKNGGLLEYTWRESTTGPPRKYYRVTQAGEELLIEL
                     LESWNQLVKVVSQTTNKQSRS"
     gene            820..2958
                     /locus_tag="PLG24_06730"
     CDS             820..2958
                     /locus_tag="PLG24_06730"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HMZ24619.1"
                     /translation="MNKTININLGGFPFTMDDDAYMLMERYLNAIKNHFAVSDSYQEI
                     MSDIESRLGELLHDKVSSKAIVTTADVEHIIKIMGRPEEFGAEAIDLPGEGSDQGTSG
                     DPIGTQRTRRLFRNLADKKLGGVCSGLAAYLGIKNANWIRLLFIILASSGGFGIPLYV
                     IMWIFVPPAKTSADYLAMKGEDINIHNIAEFLETEIKHFSNQVTDFAQDISQQFSNKG
                     KKSFRADRPSIDLEETFRSGTARLVSILRPFGLVLGGIFTLIFLGFWITLIVSYILAF
                     PLLGFINSTSPIINFLTSLNSIVIFAVPVVFVIFWALKIFFKTPVHRSIRQSLAIFWI
                     SNLICFILLSALTFREFKSYGAIEVKQDLGSLKDDQINISAITPDHRDGRLVLGDMEM
                     GNDNQLEINDNVRFNIYPTNGPAWSATKRVTSRGKDGVDAKKNADCVSYAISPKDAHT
                     LDVPTFFSIRPDCKYRFQQVEIDLFIPIGKYVTFPESLEWRLSEVKYDEAQMPNGWKF
                     IPGKRYKMSEMGLTCDDCTDEELTANMHADDYEYISQEDIPANAVVQSTEKEMDIALT
                     KDQLTTVSFDKIMNPTGHSVKFSKINLNIDQSMDDKVRIIRKLKFYSHTPIAVDQLDK
                     IYGIRQNGNDLIFSNTGLIFNDKKAFNPEITIKLLIPVGTKISFDENVNEFVEHVKFD
                     KEKITDNWHFLKNEVLEMGPKGLYCTNCND"
     gene            3192..4382
                     /locus_tag="PLG24_06735"
     CDS             3192..4382
                     /locus_tag="PLG24_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HMZ24620.1"
                     /translation="MEENKTSTSDKILWWLASADAQLLEGNRSEQRKFSLIGLSIFFT
                     WLFATAAWTYFFSTVTDSILIMLLGGFLMGGIVLTIDRVLIAGLQGSGEGSKTGAIAF
                     RVALALCLGAFMAQPALLYLFKKDINIQLTLDREVKKQAAVAEINRSLQPQLKPLLTQ
                     RDSLAGIQASLDSQVVALRNSYLAETDGSGGTGKVGIAAIARAKKAEYDAATLASNQW
                     KSSVAPLLSKINSNIDTMTSIRNKQIAAFDANDNDGFLARIEALQHLIESNLALRWRY
                     LLLLSIIMLIELAPVLSKLLLNTKVYNQRLILEEGYLLNLDREVFEKKYSEARKYSDA
                     MHQSNLDAINKIFEKSNSMMNQSSNDEMNKYELHHGKPATVNSLLTHMKQNLMGRHTS
                     SFEQ"
     gene            complement(4400..4957)
                     /locus_tag="PLG24_06740"
     CDS             complement(4400..4957)
                     /locus_tag="PLG24_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HMZ24621.1"
                     /translation="MIIAFKSFIILSFTKVNIMQILNTFQMNQKIRRISFEILERNLG
                     EEEIYFIGINNSGYALAQLLSDMLNGLEYNEIKSSVTRIRVKADQPTASDIYLEGIDV
                     NELDNKCLILVDDVANTGRTLFYAFQPLLRIIPRKLEVAVMVDRKHKLFPVHVDYVGL
                     SLATTLKENIRVDLSDIKNMTTSLN"
     gene            4950..6287
                     /locus_tag="PLG24_06745"
     CDS             4950..6287
                     /locus_tag="PLG24_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HMZ24622.1"
                     /translation="MIISQILFLILLVWVFKEAVKRYGKVIRNIRLGRNDEPLNNKGG
                     FRRMLLVAFGQQKMFKRPLPAILHLFIYVAFIITQFDLLEQIIDGITGSHRILSHYLG
                     GLYSFMISFIELLSVFAFIATIAFLSRRNLLRIPRFTSKDLAGWARIDANLILIGEIL
                     LLTGIFCLNGADKALETKFPNEYPSTGFFLITGWLGPLLFGGMPEFFLHVISGFGWWL
                     HVLVVFGFILYLPGSKHLHILLAFPNTYLSSDNPRGRMINIESIENEVKNMMEIPLNH
                     APKETLADFGVHDIFQLTQKNLLDAYSCTECGRCTAVCPANLTGKKLSPRKIMMNVRD
                     RMEEVGCKLDDPNFSSMNGEIDNKIGFNDGRDLFNYIEKEEIRACTTCNACVEACPVL
                     INPLEIILQLRRYEILTQSSGPPDWVPMYTALENNGSPWQMTIERDDWIKELN"
     gene            6293..7078
                     /locus_tag="PLG24_06750"
     CDS             6293..7078
                     /locus_tag="PLG24_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HMZ24623.1"
                     /translation="MHIKTMAEFAAEGRDPEVLFWVGCLGSYDARAQRVSAAFAQILD
                     NIGIEFAILGKEESCTGDPARRAGNEFIFQMLANQNIQTLTNYGVKKVVTTCPHCFNI
                     FKNEYPELGATFEVFHHTQFLSQLLQDKRLKIEGGSFDGQRITYHDSCYLGRINGEYE
                     APRELIHALDVDLVEMGRSREKGLCCGAGGAQMFKEDEPGEKRINTERAEEAINTGAS
                     IVAVNCSFCNVMMTDGLKAKEKQDEVKVMDVAEMIWESMKPKN"
BASE COUNT         2161 a         1466 c         1435 g         2017 t
ORIGIN      
        1 actaccccct cactcaaaaa atcgaagtag gtaggaaagt aataccatac aaccaacacc
       61 agacttacat tagagtagac caaagtttgt tttctttcat tttatttcat ccttagtttt
      121 ttccgccttt gtattttact accctgattg aattataagc tattattctt ctacttagtt
      181 cacatcgacc tgccccaata tcgttctttc caattcattc aataatgcta tcgcatctca
      241 acagattaca cactatccaa taattcattt gatgcttaac cgttactttc atcgactttt
      301 acattacatt aatctatctt ttacaaatta ttatcttaaa gatagtacta agcaatacat
      361 aataccatat atttgtgtcg ttattgatat acatgattat acattattca ttgttaccaa
      421 tatttattca cttataaatt tattcaagga tatgaatgaa aaggaaatgc cggatcgcga
      481 gacgttgaag gcagagaata ccaaagctca aatgcgcaaa ggtgtactag agatgtgtat
      541 cctcggcatc attagcggcg aagaacttta tacctccgat atcatcaata aactcaagga
      601 aaacgaactg gttgtagtgg aaggtacagt atacccatta cttacccgac taaaaaacgg
      661 cggacttctg gaatatactt ggcgcgaaag cacgaccgga cctcctagaa aatattatcg
      721 tgttactcag gcaggagaag aacttctcat tgaattactg gaaagctgga atcaactggt
      781 aaaagtcgtg tctcaaacaa ccaataaaca atcaagatca tgaataagac aataaatatc
      841 aacctcggcg ggttcccatt cacaatggat gacgatgcct atatgcttat ggagcgttac
      901 ctcaacgcca tcaaaaatca ttttgctgta tccgacagct atcaggaaat aatgtcggac
      961 atcgaatcca ggctaggtga acttctacat gataaagttt cttccaaggc tattgtaaca
     1021 acagctgatg tggaacatat catcaaaatc atgggaagac ctgaagaatt cggtgctgaa
     1081 gctatcgacc tgccgggtga aggctctgat cagggcactt ccggtgatcc gatcgggaca
     1141 caacggacac gcagactatt tcgcaactta gccgataaga aactaggtgg cgtttgttcg
     1201 ggattggctg cttacttagg tattaaaaat gccaactgga tcaggctgct cttcatcatt
     1261 cttgccagct cgggaggttt tggcattcca ctttatgtca tcatgtggat atttgttccc
     1321 cctgcaaaga catcggcgga ctatctcgcc atgaaaggag aggatatcaa cattcataat
     1381 atcgcagaat ttcttgaaac agaaattaaa catttctcca atcaggtcac tgacttcgcc
     1441 caggacattt cacaacagtt tagcaataag ggaaaaaagt cttttagggc agaccggcct
     1501 tccattgatc tggaagaaac ctttagatca ggcactgccc gccttgtaag tattttacga
     1561 cctttcggac tagtgctggg tggaatattc acattgatat tccttggatt ttggatcact
     1621 ttgattgtca gttatatcct cgcttttcca cttctgggtt tcattaactc cacctctcct
     1681 attattaatt tcctgacctc tctcaacagt atcgtgattt ttgccgttcc cgttgtgttc
     1741 gtcattttct gggctttaaa aatattcttc aaaacccccg tccaccgcag tattcgtcaa
     1801 agtctggcta tattctggat ttctaaccta atttgcttta ttctactgag tgcattgacc
     1861 ttcagagaat tcaaaagtta tggagcgatt gaagtgaagc aggatctggg aagtctgaaa
     1921 gacgaccaga ttaacatctc cgcgataact ccggaccata gagacggacg attggttctt
     1981 ggagacatgg aaatgggcaa tgacaaccag ctggaaataa acgataatgt aagatttaac
     2041 atctatccta ccaacgggcc tgcatggtca gccaccaaaa gggtgacttc acgtggtaaa
     2101 gatggagttg atgcaaaaaa gaatgctgac tgcgtaagtt atgcaatttc gccaaaagac
     2161 gcacacacgc tcgatgttcc tacattcttc agcatcagac ctgattgcaa atatcgtttt
     2221 cagcaagtgg agattgatct tttcattcct ataggtaaat atgtgacatt tccggaaagt
     2281 cttgagtggc gcctgtctga ggtaaaatat gacgaagccc aaatgcctaa tggctggaag
     2341 tttatacctg gtaaaagata taagatgtca gagatgggtt tgacttgcga tgattgtacc
     2401 gatgaggaac ttactgccaa catgcatgct gatgactatg aatatatcag tcaagaagat
     2461 attccggcaa acgctgtagt tcaatctact gaaaaagaaa tggatatcgc cttaactaaa
     2521 gaccaactaa ctactgtttc tttcgataaa atcatgaatc caaccggcca ctcagtcaaa
     2581 ttttccaaga ttaatttgaa tattgaccaa agcatggacg ataaagttcg aattattaga
     2641 aaacttaaat tttatagcca tacaccaatt gctgtggacc aattggataa aatctatggt
     2701 ataagacaaa atggtaatga cttgattttc tccaataccg ggctcatatt taatgataaa
     2761 aaagccttca accccgaaat cactataaaa cttctgatcc ccgtaggcac aaaaatttcc
     2821 tttgacgaaa atgtgaatga atttgtggaa catgtcaaat ttgacaagga aaaaataact
     2881 gacaattggc acttcttgaa aaatgaagtt ttggaaatgg ggccaaaagg tctttattgt
     2941 acaaattgca atgattaatc aattactgtg atttacgaag aatctttata atgcctgtaa
     3001 tacataattt gtattgcagg catctataat attgtatggg ataacaaggt attacactac
     3061 aattgaaacg acagggacat actatttata cgtaataaat atgatttacg gctatgttta
     3121 cgttattcta tttaaatctc tagagcgaca ggccaccttt accaaattta tttttcaact
     3181 tttttaatct tatggaagaa aacaaaactt ccacctcaga taagattctg tggtggcttg
     3241 catccgctga tgcacaattg ttggagggaa atcgatccga gcaacgaaaa ttcagtctga
     3301 ttggactttc tatcttcttt acctggctgt ttgcaactgc tgcctggaca tatttcttca
     3361 gcactgtaac agacagtatt ctgattatgc ttctaggggg cttcctgatg ggaggtattg
     3421 tgctgaccat cgacagggtc ctgattgcag ggcttcaagg ttcaggggaa ggaagtaaga
     3481 cgggcgccat cgctttccgt gtagctctgg ccctttgcct tggtgccttt atggctcagc
     3541 cggctttgtt atatttattc aaaaaggata ttaatatcca gctaacactt gacagggagg
     3601 taaaaaaaca agccgctgta gcagagatca accggtcttt acaacctcaa ctaaaaccac
     3661 tcctcaccca aagagattct ctggcaggca tacaggcatc tttagacagt caggtcgtag
     3721 ccctcagaaa ttcttatctt gctgaaacgg atggtagtgg tggaacaggc aaggtaggca
     3781 ttgctgcaat agcacgcgca aaaaaagcgg aatatgatgc tgctacattg gcttccaatc
     3841 aatggaagtc atcagtagct cccctgctta gcaagatcaa ttccaatatt gataccatga
     3901 catcaatccg gaataaacaa attgcagcct ttgatgctaa cgacaatgat ggctttctgg
     3961 cacgtattga ggcgctccaa catttaatcg aatcaaacct tgccctcaga tggcgttatt
     4021 tattattgct ctccatcatc atgttgatcg agttagcacc ggtgctaagc aagttattgt
     4081 taaacacaaa ggtatataac cagcgattaa ttctcgaaga agggtaccta ctcaaccttg
     4141 acagggaagt gtttgagaaa aaatacagcg aagcaagaaa atattccgat gccatgcatc
     4201 agtccaatct ggatgccata aataagattt tcgaaaagtc caactcaatg atgaatcagt
     4261 catcaaatga tgagatgaat aaatacgaat tgcatcacgg caaacccgct acggtcaata
     4321 gcttgctaac tcatatgaaa caaaacctga tgggccggca cacctcttcg ttcgaacaat
     4381 gaaaaaaatt tgagcgccgt caattgagag aggtagtcat attcttgata tctgacaggt
     4441 ccactctgat attttctttc agggtagtcg ccagtgacag gcctacatag tcaacgtgga
     4501 caggaaacag tttatgtttt ctgtccacca tgaccgctac ctcaagcttg cggggaataa
     4561 tcctcaataa tggttgaaag gcataaaaca acgtcctgcc tgtattagcc acatcatcta
     4621 ccaatattag acatttatta tccagctcat tgacatcaat cccctccaga tatatgtcag
     4681 aagctgtcgg ctgatcagcc ttcacacgaa ttcttgtgac ggaggattta atttcattat
     4741 attccaaacc attaagcata tctgacagca gttgcgccag ggcatatccg ctattgttga
     4801 tccctataaa atatatttcc tcctcaccaa gattccgctc caggatctca aaagaaatcc
     4861 ttctgatctt ttgattcatt tggaaggtat tcaatatttg cattatattt acttttgtaa
     4921 aggataaaat tataaaagat ttaaaggcga tgatcatttc tcagatactt tttttaattc
     4981 ttcttgtctg ggtgttcaag gaagccgtaa agcgctatgg caaggtaata aggaatattc
     5041 ggctcggtag gaacgacgaa ccgttgaata acaagggtgg gttccgccgt atgttgctgg
     5101 tggctttcgg tcagcaaaag atgttcaaac gtccacttcc ggcgattttg cacctattta
     5161 tatatgtggc atttattatc acacaatttg atttgctcga acagataata gacgggatta
     5221 ctggaagcca cagaatatta tcccactatc tgggtggatt atattcattc atgattagtt
     5281 tcatcgaatt gttgtccgta tttgctttta tcgctacaat agcctttttg tcaaggcgca
     5341 atttgctccg gatccctcgc tttaccagta aggatcttgc agggtgggcc aggattgacg
     5401 ccaacctcat ccttataggg gagatccttc tcctcaccgg tattttctgc ctcaatggtg
     5461 ctgataaagc cctggaaacc aaatttccaa atgaatatcc ttccacagga ttctttctga
     5521 ttaccggctg gttaggcccc ttgctctttg gaggcatgcc tgaattcttc ctccatgtaa
     5581 tcagtggttt cggttggtgg ctacatgtac tggtcgtttt cggattcata ctatatcttc
     5641 ccggatcaaa gcacctgcat attttactgg cattcccaaa tacttatctt tcatccgaca
     5701 atccacgagg aagaatgatc aacatagagt ccatcgagaa cgaagtaaaa aatatgatgg
     5761 aaatcccgtt gaatcacgcg ccgaaagaga cattggccga ttttggtgta cacgatattt
     5821 tccagttaac gcaaaaaaac cttctggacg cttattcatg tactgaatgc ggacgttgca
     5881 ctgctgtgtg cccggccaac ctcaccggaa aaaaattatc gccccgcaaa ataatgatga
     5941 atgtccgcga ccgcatggaa gaagtaggtt gcaagcttga tgatccaaat ttcagttcca
     6001 tgaatggtga aattgataat aaaattggat tcaatgatgg ccgagactta tttaattaca
     6061 tcgaaaagga agaaataagg gcctgtacaa cctgcaacgc ctgcgttgag gcctgtcctg
     6121 tcctcatcaa tccacttgaa atcatccttc agctaagacg ttacgaaatt ttgacccaaa
     6181 gctccggtcc accggattgg gtaccaatgt acacagcact tgaaaataat ggaagcccgt
     6241 ggcagatgac cattgagcgg gatgattgga taaaagaact aaattaataa atatgcatat
     6301 caagacaatg gcagaatttg cagcggaagg tagagatcct gaagtgttgt tttgggtagg
     6361 atgtctgggc agttatgacg ccagagcaca gcgagtctca gctgcttttg ctcagatact
     6421 ggataatatt ggcattgaat ttgctatcct tggtaaagaa gaatcttgca caggagatcc
     6481 tgcccgacgc gccggaaatg aatttatctt ccagatgctt gccaatcaaa atattcagac
     6541 tctgacaaat tatggagtaa aaaaagttgt taccacctgt cctcattgtt ttaatatatt
     6601 taaaaatgaa tatccggaac ttggggcaac gtttgaagtg ttccaccata cacagttcct
     6661 aagccagttg ttgcaggaca aacgattgaa aattgaaggt ggatcctttg atggacagcg
     6721 tattacatat cacgattcct gttacctggg acgtatcaac ggtgaatatg aggccccccg
     6781 tgaattaata cacgctctgg atgttgatct ggtagaaatg ggacgaagcc gcgaaaaagg
     6841 actttgctgc ggtgcaggag gtgctcagat gtttaaggaa gatgagccgg gagaaaaacg
     6901 tatcaatacc gaacgtgcag aagaagcaat caatacagga gcgtccatag tcgctgtcaa
     6961 ctgctccttc tgcaatgtca tgatgaccga cggtttgaag gcaaaagaga agcaagatga
     7021 agtgaaagta atggacgtag ctgaaatgat ctgggaatca atgaaaccaa agaattgat
//
LOCUS       DAOQQN010000147         7073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4364_length_7073_cov_5.619692, whole genome shotgun sequence.
ACCESSION   DAOQQN010000147 DAOQQN010000000
VERSION     DAOQQN010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4364_length_7073_cov_5.619692"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PLG24_06755"
     CDS             complement(<1..643)
                     /locus_tag="PLG24_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HMZ24624.1"
                     /translation="MRLINILLLFSIVTFYCMGLHGQVLEGSGSAPGSYLIQFDSGDA
                     AASFSKRMTTERKGVTLESCGINSNILLVTGVDLDWDELTLLVSNSDGVIAFQRDYYL
                     ENRSKEPNDPWFKTKQWDMKTIKGPEAWEITTGGVSPLGHQIVVGVIDNGFFVNNADL
                     HANLYVNPGEIPDNGIDDDGNGYIDDVRGVNIGNGTGIHAEASHGTGVLGIIGA"
     gene            complement(864..2426)
                     /gene="lepB"
                     /locus_tag="PLG24_06760"
     CDS             complement(864..2426)
                     /gene="lepB"
                     /locus_tag="PLG24_06760"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HMZ24625.1"
                     /translation="MSILIFLFVSYILLCLSLKPLFVAAGFPGIDAYIPGKNFATWCK
                     LIGRKPSYALWLLFPVVNIFIFCSMAVDMVLSFGRNSFKDSALAVIYAPLKFFLMHNA
                     GDTYKGPAVTIVRQYHQDLKSAYERKDNFAIKKLESNPPFPKQKAREWTESIVFAVFA
                     AAFIRMFLIEAYVIPTSSMESSLRVGDYLFVSKAHYGIRMPMTVAMVPLIHNRLPLVN
                     RESYLKSPSLPYYRLPSITPVKRNDPVVFNYPEGDSVYVTPGRTYSIYDVRRDPRLAQ
                     GYPLTTRPLDKMDHYIKRCIGMPGDTLKIVAKGVYINGSRIEDPSGMQVSCRVTPSQP
                     VPLSDLADMGVNLNECNPEVGYYSLTKSVMEYIKKEVPSVQIEILYANPQQYAAAMFP
                     HAPKQFPAWTPDNYGPVWIPKKGVTINISEDNIAFYRRVISVYEKNNLEEKNGKFYIN
                     GTETNQYTFKQDYYWMMGDNRDNSEDSRFWGFVPEENIVGKPLFIFFSKYSDMNGSKI
                     RWNRIFSGANKF"
     gene            complement(2562..3131)
                     /locus_tag="PLG24_06765"
     CDS             complement(2562..3131)
                     /locus_tag="PLG24_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24626.1"
                     /translation="MSKRSLYLQVTIVTLLILITAFSRLIPHNMNFTPVGAIALFGGA
                     YLGRNWKAFLIPVLVIFISDLALYQFVYADAGYSFFYDGIAWVYGSYLLIVLLGAYWI
                     KKVNVFRVLTGSLGAAVLFFLVTNFGCWPGTTYTPDFNGLMTCYAAGVPFFKYTLAGD
                     LIYGLVLFGTYALIKNKLSIQSATDIQSA"
     gene            complement(3690..5873)
                     /locus_tag="PLG24_06770"
     CDS             complement(3690..5873)
                     /locus_tag="PLG24_06770"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HMZ24627.1"
                     /translation="MPHFQCSICYKLVMILIFLDVTAQAKVIKMTSPGGNVSSVFQLE
                     SDGEVFYSAAAMGDVFIKKSSLGVVIEGRKWFEANILLHVDSTTVDETWEPVLGEEAT
                     IRNHYKEYVFHLCDLVDKDLHYKIIFRLFDDGLGFRYEFPEQHKLTYFVLKDELTEFA
                     LSQDCMAFWLPGDYEEHSLDYQTTRISKITGLNKSNGLSNNKTAVSDNLIQTPLLLKS
                     PSGLYINIHEAALIDYPAMNLKVDPVGMKLKVNLTADAVGNKAYLQTPCFSPWRTVIA
                     CKKAGDILASRLILNLNTKRKNGDYSWIKPIKSIGVWREMRLGRSTWSYSDKVNVALI
                     SNYLPSLGPNGKHGANEVNVKKYLDFAAGNGFNGVLVEGWNLGWEDWYGHWKERVFDF
                     VTPYPDFNIKAISEYAQSRNIHLLMHHETAGSVADYERSLDTALTFMRTYGYPFVQTS
                     YTGRIIPRGEQLGGQWMSSHYHRTADKLMRKKLMWNNSSMGRPTGLSRTYPNILCEGV
                     VTDKPYGRQSYGVNPDLHTILPFTKSVGGPIFFPAGILQPVKDESGDTHLHTTLARQL
                     ALFITLYSPFQLAGDLPENYELFKDAFRFIKEVAMDWSRTLYLDAQPGEFLYVARKEK
                     HGDRWFVGAITDQTSRSASLPLKFLDAGTKYKATIYADGAGAHFKTNPTAYKITEVLV
                     DSRTKLPIYLAPGGGTAVSLQYASAAEIKKLPKLSGTFLKSWKRK"
     gene            complement(6483..>7073)
                     /locus_tag="PLG24_06775"
     CDS             complement(6483..>7073)
                     /locus_tag="PLG24_06775"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HMZ24628.1"
                     /translation="KMIILASPSGAYYSKPVKLWAEDHFVRAVKGGVGEAKTAGNYAA
                     SLYPAKLVKERGYDQILWLDGIYNKYVQEVGTMNIFFVLNDKVITPAEDGAILKGVTR
                     MSLIQILKSRGIPVEEKLITIDEVFDEYEKGNLVEIFGAGTAAVVTNVASLTYKDKTI
                     TFEESSWSLSGNLKNTINQLRSGEVEDTFHFTVRVE"
BASE COUNT         2259 a         1541 c         1277 g         1996 t
ORIGIN      
        1 tagcaccaat aatacccaga actcctgtac catgactagc ctcagcatga atccccgtac
       61 cattgcctat attgacgcct ctgacatcat cgatatatcc attgccatca tcgtcaattc
      121 cattatcagg aatttctcca gggttgacat ataaattggc gtgtaaatct gcattattga
      181 caaaaaaacc gttatctata acccctacaa cgatctgatg ccccagtgga gaaacacctc
      241 cggtagtaat ttcccatgcc tcaggcccct tgatagtctt catatcccat tgcttggttt
      301 taaaccaggg gtcattaggt tctttcgacc tattctcaag ataataatct ctttgaaaag
      361 ctataactcc atcagaattt gaaactagca aggtaagttc gtcccaatcc aaatcgactc
      421 ctgttaccag taggatgtta ctattgatac cacaggattc taatgtcact ccttttcttt
      481 cagtagtcat tcttttggaa aaggaagccg cagcatcacc cgaatcaaat tgaatcaaat
      541 aactgccggg ggcactccct gatccttcca atacttgacc atgcaggccc atacagtaaa
      601 atgtcactat tgaaaataaa agcagaatat taatgagtcg catatatata gaaattcatt
      661 aatgcaaatc taatcaaaaa ggtaccagaa ggcaaaaaaa taatattttt tcacaaaaag
      721 gaaccattct atatcaaccg acccaaataa tgccatcctg atcaggtatg tatggatact
      781 ccctccgggt gcttacctcc aggcaaccat tatttaaaag aatttccggt ataatgacaa
      841 ctttgtcgac ccttctggcc gtgttagaat ttattggccc cggaaaatat tctgttccac
      901 ctgattttac ttccattcat atcactgtat ttggaaaaaa agatgaaaag aggtttgcct
      961 acaatattct cctcaggcac gaagccccag aacctggaat cttcagaatt atccctgtta
     1021 tcgcccatca tccaataata atcctgtttg aaggtatatt ggtttgtttc agtgccgttg
     1081 atataaaact ttccattttt ttcctccaga ttattcttct catagacact gataactctc
     1141 cgataaaaag caatgttatc ctcactgata tttattgtaa cacccttctt tggtatccat
     1201 acaggaccat agttatcagg ggtccaggca ggaaattgct ttggtgcgtg tggaaacatc
     1261 gccgcagcat attgttgggg gttggcataa agtatttcaa tctgaacaga gggtacttct
     1321 ttcttaatgt actccattac agactttgtc agtgaataat atccaacttc gggattacat
     1381 tcattgagat taacacccat atcggccaaa tcggacaatg gaaccggctg acttggagta
     1441 acacgacagc tgacctgcat ccccgaaggg tcttcaatcc gggatccatt gatataaaca
     1501 cctttcgcta caattttaag agtatctccg ggcataccaa tgcatcgctt gatatagtga
     1561 tccatcttat ccaggggtct tgtcgtaaga ggataaccct gagccagcct gggatctcgc
     1621 ctgacatcgt agatactgta ggttctgcct ggggttacat acacactatc cccttcaggg
     1681 taattaaaga caaccggatc gtttctttta accggagtga ttgatggcag tctgtaataa
     1741 ggcagacttg gacttttcag ataagattca cgattgacta atggaagtcg gttatggata
     1801 agtggtacca tagcaacagt catcggcatc cggattccat aatgcgcctt gcttacaaac
     1861 aaatagtcac ccacccttaa ggaagattcc atggaagaag taggaataac ataagcttca
     1921 atcaaaaaca tgcgaatgaa ggcagctgca aatacagcaa aaacgattga ttcagtccac
     1981 tcacgcgctt tttgctttgg gaaaggtggg ttggattcca atttttttat agcgaagtta
     2041 tccttacgct cataggctga cttcaagtcc tgatggtatt gcctgacaat cgtaacggca
     2101 ggccctttat aagtatctcc cgcattgtgc atcaaaaaga acttgagcgg ggcataaata
     2161 actgcaagtg cactatcttt aaagctattt ctaccaaacg acaataccat atccactgcc
     2221 atactgcaga atataaatat gttaactacc ggaaaaagaa gccataaggc ataggagggc
     2281 tttcgtccga tcaacttgca ccaggtcgca aaattcttac cgggaatata tgcatcaatc
     2341 cccggaaatc cggcagctac aaataatggt tttaaactaa ggcagagaag tatgtatgaa
     2401 acgaaaagga atataaggat actcacaggc aataatgtat ttgatttgaa ttacaaagat
     2461 aaacgtatat tgatagctta ccctttagtt gacggtaaaa ttcgcctaat atcaaatata
     2521 acaagacgaa aagaaatgaa gaagatataa aatcaattta gtcaggcaga ttgtatatca
     2581 gtggcagatt ggatacttaa tttgtttttg atgagagcat aagtcccaaa taataccaag
     2641 ccgtatatca aatcgccagc caatgtgtac ttgaagaaag gcacaccagc tgcataacaa
     2701 gtcattagtc cgttaaaatc aggtgtgtaa gttgttcccg gccagcatcc aaaattcgta
     2761 accagaaaaa acaatacagc tgctccaaga gatcctgtta ataccctgaa cacatttact
     2821 tttttgatcc aatatgcacc taataatacg atcaaaaggt aggacccata cacccaggca
     2881 attccatcat aaaaaaacga ataaccggca tcagcatata caaattgata taaggccagg
     2941 tcactgataa agataacaag aacaggaata agaaaagctt tccagtttct gccaagatag
     3001 gcgccgccaa aaagtgcaat ggcaccaacc ggagtgaagt tcatattatg gggaatcaag
     3061 cggctgaaag cagttattaa aatcaacaaa gtaacaatgg tgacctgaag atataaagac
     3121 cgtttggaca tagtagctgt aattttttac aaagttataa aaaatctgat aagaaaaagc
     3181 ttcatatatt attttgggtt gcatatcgac cggagcacat aggataaaat cctggaatta
     3241 ttcctctttg tttagcccgg atcattcacg attccttcaa catccaaagt ccttattccc
     3301 caaataaaat ggtagttaac attagtcggc aaaacacacc acaataaccg gcaatagaca
     3361 cagtttcctg ttataacctg tagaatacca cttagtacac tgaatttatt ttacgctgcg
     3421 atgcgacatt aaaggcatta cagttgcatt gcacactctt tgggaagaaa aaaaagagct
     3481 ttcctgatca aggaaataat ctttgaatta aaaaatgatt ttaaatagag caaatcattt
     3541 aaaaggtcaa aacggttaat tttatctagt tgccggatgg ttctgacaat tacttggaag
     3601 attaatgcaa aagcaaagca ccaaaaacat ccttccggta gaccaactta cctatttcat
     3661 atcatatact tacaaaacac gtatcctgct catttcctct tccaggattt cagaaaagtt
     3721 ccggataatt ttggcaactt cttaatttca gcggcactcg cgtattgcaa agagaccgct
     3781 gttccaccgc ccggcgccag gtatattggc aattttgtgc gggaatcaac caacacttcg
     3841 gttatcttgt atgcagtcgg gttggttttg aaatgggctc cggcaccatc tgcgtaaatg
     3901 gtagctttat atttcgtacc tgcatcgaga aattttagag gaagtgaagc cgatcttgat
     3961 gtttgatcag ttatagcacc tacaaaccac cggtcaccat gtttctcttt ccttgccaca
     4021 taaaggaact ctccgggctg cgcatccaaa tatagggtac gagaccaatc catggccact
     4081 tctttaataa aacggaatgc atccttaaat aattcatagt tttcgggcag gtcgcctgcc
     4141 aattggaatg ggctgtataa ggtaataaag agcgctagct gacgtgccag ggtagtatgg
     4201 agatgcgtgt cgcctgattc atccttaaca ggttgcaaaa tccctgccgg aaagaaaatc
     4261 gggcctccaa cagatttggt aaatggcaga atagtgtgca aatccggatt tacaccataa
     4321 gattgcctgc catatggctt atccgtaact actccttcac aaaggatatt aggataagtg
     4381 cggctaagtc ctgtcggcct gcccatactt gaattattcc acattaactt ttttctcatt
     4441 aatttatccg ctgtcctgtg ataatgagat gacatccatt gacccccaag ttgctctccc
     4501 cgtggaatga tgcgtccggt atatgatgtc tgtacaaagg gatagccata tgtacgcata
     4561 aaagtcaaag ccgtgtcaag agaacgttca taatcggcca cagaacccgc tgtttcatgg
     4621 tgcattagca aatgtatatt tctggattgc gcatattcag aaattgcctt aatattaaaa
     4681 tcaggatatg gcgtgacgaa atcaaaaaca cgttctttcc agtgaccata ccaatcctcc
     4741 cagccaaggt tccaaccttc tactaaaaca ccattaaagc catttccggc ggcaaaatcc
     4801 aagtatttct tcacattcac ttcattggca ccgtgcttgc catttggtcc aagagagggc
     4861 aggtagttac taatcaatgc aacattaact ttatcggaat aactccatgt agaacgtcca
     4921 agtctcattt cacgccatac tccaatgctt ttaatcggct taatccacga ataatcacca
     4981 ttcttacgtt tggtgttcag gttaagaatc agtcgggatg caagtatatc tccggccttt
     5041 ttacatgcaa tgaccgttct ccaggggcta aaacatggcg tctgaagata agccttattt
     5101 cctacagcat cagcggtaag gttcactttc agcttcattc cgacaggatc aacctttaga
     5161 ttcatagcag gataatcaat caatgcagcc tcgtgaatat taatatacag gccggatggg
     5221 gattttagca acaatggagt ttgaatcaag ttgtcactga cggcagtttt attatttgaa
     5281 agcccattgc ttttattaag tccggtaatc ttggagattc gggtggtttg ataatccaac
     5341 gaatgttcct catagtctcc cggaagccaa aatgccatac aatcctgact cagtgcaaat
     5401 tcagtcagtt catccttcaa aacaaagtag gtcaatttgt gctgctcggg aaactcatac
     5461 ctaaacccaa ggccatcgtc gaacaatcgg aaaataattt tgtaatgaag atctttatcc
     5521 accaaatcac aaagatgaaa tacatattcc ttgtagtgat ttctgatagt cgcttcctca
     5581 cctaaaacag gctcccacgt ctcatcaaca gtcgtagagt caacgtgcag aagaatattg
     5641 gcctcaaacc acttccttcc ttcaataact acacctaaag acgatttctt aataaagaca
     5701 tcgcccatag ccgcagcaga gtaaaatact tcaccatctg attctaactg aaaaacagat
     5761 gacacattac ctccaggcga agtcattttg attaccttag cttgagctgt aacatccagg
     5821 aaaatcaata tcatcaccag cttataacaa atagagcact gaaagtgtgg cattggctta
     5881 ataaatttga ttacaaggta ttcctcatcc gggaacaacc cggctgaaag acttatgatg
     5941 agaccaaacg aatacttact gaactgttat caaattgcaa tttattatac tgatattcta
     6001 atatttatat atattataaa ataatattat atttgaataa acatgaaatc aattgacgac
     6061 aaattcatta caagtataag aaatattcaa ttaatacata taaatggatc atgaatgtat
     6121 aacatttatt gattccctca tcaatcgttt gtgcataaac aaatgatata gtataagtta
     6181 ccaattggca ttgaacagcc ttaagaagag gactaaagcc agatttttgg ctattaagac
     6241 aggaaagtga atccaaccga cactttttcc tctgtacctt gtatttcttt ataacctcaa
     6301 ttaacagagc actctacata gcccataaat tgaagataag tacaaagttc gaaactcaca
     6361 aataagggaa ggcttagaaa tagccataaa aaaatggtcc tgatacaaaa atcaggacct
     6421 taaagtattc ttagtgttat aaattgaata gcaattgatg aaactcattg ccggcagcat
     6481 aatcactcta cccgaaccgt aaaatgaaag gtatcttcca cctctcccga tctgagttga
     6541 ttaatggtat tcttgaggtt gccggaaagg ctccaggatg actcttcaaa tgttatagtc
     6601 ttatccttat aggtcagaga agcgacattg gtcacaacag ctgctgtacc agctccgaag
     6661 atctccacca gattgccttt ttcatattca tcaaatacct catcaatagt aattaacttc
     6721 tcttccacag gtataccccg ggacttcaaa atttggatta aagacatccg ggtcacaccc
     6781 ttcagaattg ctccatcttc ggccggtgta ataactttgt cattcaaaac aaaaaatatg
     6841 ttcattgtac caacttcctg gacgtatttg ttgtagattc cgtccaacca taatatttgg
     6901 tcataacctc tttctttaac caattttgca ggataaagag atgctgcata gtttccggcg
     6961 gttttagctt ctcctactcc accctttact gcccggacaa aatgatcctc tgcccataac
     7021 ttcacaggct tggaataata ggcaccggat gggcttgcca atatgatcat ttt
//
LOCUS       DAOQQN010000148         7053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4395_length_7053_cov_3.933695, whole genome shotgun sequence.
ACCESSION   DAOQQN010000148 DAOQQN010000000
VERSION     DAOQQN010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4395_length_7053_cov_3.933695"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2888)
                     /locus_tag="PLG24_06780"
     CDS             complement(<1..2888)
                     /locus_tag="PLG24_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMZ24629.1"
                     /translation="MASLSTISVNRPVLAIVMNLIILLFGLIGFKTLGVREFPAVDPP
                     IINVRTNYPGANADIIESQITEPLEKAINGIQGIRTITSASNQGSSNITVEFDLSSDL
                     ESAANDVRDKVSQAIRQLPKDLDGVPIVSKADANSDAIISMTVQSNTRNHMELSDFAE
                     NVLGPRLQTIPGVSSLQIWGQKKYAMRLWLDPVKLSSFHLTAIDVTNALQKQNVELPS
                     GKISGDETELTIKTIGRLSTPEEFNNMIIKSFPDGRDIKLKEIGEAMLGVENEETILR
                     EKNIPMIALALVPQPGSNYVAIADEFYKRLDQIKKDVPKDISLNIAMDNTRFIKQSIV
                     EVEETFLIALSLVVLIIFLFFRSWIVAVRPLIDIPVSLIGSFFVMYLMGYSINVLTLL
                     AIVLATGLVVDDGIVVTENIYKKIERGMNPKRAAIEGSNEIFFAVISTSLTLAAVFMP
                     VVFLQGFVGRLFREFGIVLASSVLISAFVSLTLTPMLNAYLTKKSNEHGWFYRITEPF
                     FQKMESGYKNALESFLKVRWWSFVIILGAIGMIYFYMGRLKSELAPLEDRSMFRLSVT
                     APEGSSFEFTDGIIKSIVSTLQDSIPELDVALSVTAPGFMGSGAPNTGFVRIRLVEPA
                     FRIRTQQQVVDHVNRLVKRFTNAKVFVLQDQTISAGGGPRGGLPVSFIIQAPNFEKLE
                     AIIPEFLNKVNADPTFQGNDINLKFNKPELNVTIDREKAKTMGISVGDVAQVLQLAFS
                     GQRYGYYIQNGKQYSVIGQFDRINRDAPLDLKSLYLKTSSGQIVQLDNVVKVEELSSP
                     PQLYHHNLYQSATISAGLAPGKTIAEGIQAMDKIADQVLDESFSTDLSGASRDYAESS
                     SNIMFALVLALILVFLILAAQFESFIDPFIIMFTVPLAFAGAVFSLWYFNQTMNIFSQ
                     IGIIMLIGLVTKNGILIVEFANQIRERGNSKMDAIIEGATARLRPIIMT"
     gene            complement(2891..3955)
                     /locus_tag="PLG24_06785"
     CDS             complement(2891..3955)
                     /locus_tag="PLG24_06785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ24630.1"
                     /translation="MRKYIVLITITAIVAALFVAKFLFFPAQEEQKMGFMAGGKKSGP
                     LGVDIQVLKSEKANGTLQSTGTILANEKVDLSPEAAGKVSGIYFKEGGLVKAGQLLVK
                     LNDADLKAQINKSRLNLKLAEDKAARQEKLLAIQGTSKEDYDIAHNEVLSFKADIAYY
                     QTLIDKTELRAPFSGIVGFRNIALGSYVSPSVVVTTLQQLDPIKIEFTAPEKYLANFK
                     VGQKFNFYSEASKKTLTGTVQTIDPQIDLNTRSVIIRGVAPNVANLLPGNFVKVDINI
                     SQESDVLRIPSSAVVPILKGQKVYLYKNGKVVEKKIEILGRDDKTVQVSNGLNAGDSL
                     IITGIIMLKEDMDVKPDKVK"
     gene            complement(4040..4450)
                     /gene="mce"
                     /locus_tag="PLG24_06790"
     CDS             complement(4040..4450)
                     /gene="mce"
                     /locus_tag="PLG24_06790"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HMZ24631.1"
                     /translation="MIRLEHVGIAVSNLKEANELFEKLFGRKAYKEEHVDSENVTTSF
                     FQLGESKIELVGSDPEVETAISKYISKKGQGIHHLAFEVDDILESMQEMREKGFILLN
                     EEPKPGADNKLICFLHPKSTNGVLVELCQSIKSR"
     gene            complement(4530..6701)
                     /locus_tag="PLG24_06795"
     CDS             complement(4530..6701)
                     /locus_tag="PLG24_06795"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HMZ24632.1"
                     /translation="MNSKYFTLLLLIFCAMNVSAQRSVKNVSTSVGQCVVSPINKSGE
                     TEKTHLVKIETKKNQPIIRGLRKLGLVEEFTNASCGPCAAQNPAFNTLIDANEGKIIA
                     VKYQVWFPGFDPMFETNKEEVQAKWSTYEDYTVGLYGSDNGLGGVPTAFLDGYMGNGN
                     YGGGNWNSAYYGAPVGYNQAAFNYAAAQVSPLDIQVSHSYNNTIDSVNITVIVTNNSD
                     TTFNISGYNLHAYLLESKLNWPVAPGSNGEKDFSHVFRKAASSIFGDNVLPASFASGE
                     SATFTYTVALKNYFFALNQIEALAFIQKTTDLSVMNAALSEPVSFPAGTTVGDPGVAN
                     SSKSSSEKCPAEYTAEPIIKITNQNPDKSAITQVTVAYSLNGEEVEKSFDTNIEFGKS
                     ENISFGSIPVPGGTNQIDVWIVSFNNGAKDVNLINNLTETYSFTYLSEHGAPAPKSYD
                     MEGDGALGKTLFLDDANFDYIFTMTPNLTNPPANRGGYGKSANSIILFFFQWNPASTI
                     ASMSFISDKINMPSNPVYTYDWAYTGYLENGVKYDGDKVEMFYSTDCGATYKKFKTLS
                     GNTMKTAPDKTALFLPTASEWRTDTVALSELANTDDVIFKTTVTSNWGNAGYLDNIFV
                     GQGATNTVKGINSFENVSVYPNPTADQLNVRINAKNPSTATVTILDYTGKTVLTLNNS
                     QTIFNGSNNLSFDVSSLSSGTYLLKIENKEGVNIQRFTVFE"
BASE COUNT         1960 a         1462 c         1454 g         2177 t
ORIGIN      
        1 gtcatgatga taggcctcag ccttgctgtt gctccttcga tgatggcgtc catcttactg
       61 ttaccgcgct cccggatttg attggcaaac tctacaatca ggataccgtt tttcgtaact
      121 aagccgatga gcatgataat tccaatttga ctgaagatat tcatcgtctg gttgaagtac
      181 caaagagaaa aaacggcacc agcaaaagcg agcggtactg tgaacatgat gatgaaaggg
      241 tcaatgaaac tttcgaattg tgctgctaat attaggaaaa ccaatatcaa ggccaggacc
      301 agggcaaaca taatattgga cgaagattca gcgtaatctc tggaggcacc gcttaggtca
      361 gtcgagaatg attcatccag cacctgatcg gcaatcttgt ccatggcctg gataccttca
      421 gcaatagtct ttcccggggc gagtcctgcc gatatcgttg ctgactgata gagattgtgg
      481 tggtatagct ggggcgggct gctcagctct tctactttga cgacattatc caattggacg
      541 atctgtcctg aacttgtttt gaggtaaagt gatttcaggt ccaaaggagc atcccggttg
      601 atacggtcaa actggccgat tactgaatat tgctttccat tctggatata gtatccatat
      661 cgttgtcctg aaaatgctaa ttgtaatacc tgtgcaacat cgccaacaga aatacccata
      721 gttttggctt tctcccgatc gattgtcaca ttcagttccg gcttgttgaa tttcaggttg
      781 atatcatttc cctgaaaggt agggtctgca tttactttgt tgaggaattc aggtattatg
      841 gcttccagct tttcaaaatt aggtgcctga atgatgaatg atacgggcag gccgcctcgg
      901 gggccaccgc ctgcggaaat tgtttggtcc tgtaagacaa agactttggc attggtgaag
      961 cgttttacaa gccgattgac atgatctacg acttgttgct gagtccgtat tctgaatgct
     1021 ggttctacaa gtctgatcct gacaaaaccg gtgtttggag cgccgctacc cataaatccc
     1081 ggtgctgtca ccgataaggc tacatccaac tcaggaattg aatcctgcag cgtagagacg
     1141 atcgatttga ttattccatc cgtaaattca aacgatgatc cttccggggc tgtaactgac
     1201 agccggaaca tactcctgtc ctcaagggga gccagttcac tttttaatct gcccatatag
     1261 aaataaatca tgccgatagc tccaaggata attacaaacg accaccatct caccttcaga
     1321 aaactttcca gggcattttt atagcctgat tccatttttt ggaagaaagg ttctgttatc
     1381 cgataaaacc atccatgttc gttggatttt ttagtcagat aggcattgag cataggagtg
     1441 agggtgagcg aaacgaaggc tgaaatgagt acacttgagg caaggacaat cccgaattca
     1501 cggaataatc taccgacaaa gccctggagg aagactacag gcatgaatac agctgcaaga
     1561 gtgagcgaag tggagatcac tgcgaagaag atttcattgg atccttctat cgctgccctt
     1621 ttgggattca ttccccgctc gattttttta tagatatttt ctgtcaccac gattccgtca
     1681 tctacgacaa gtccggttgc gagaacgatt gccagtagtg taagtacgtt gatagagtat
     1741 cccattaagt acatgacgaa gaaagaacct atcagcgaaa ccggaatatc gatcagtggt
     1801 ctgacagcaa cgatccagct cctgaaaaac aggaaaatga taaggacgac gaggcttaat
     1861 gcaatcagga aggtttcttc cacttccaca attgattgtt tgataaatcg ggtgttatcc
     1921 attgcgatat tcaggctgat atcttttgga acatcctttt taatttgatc aaggcgtttg
     1981 taaaactcgt cagctatggc gacatagtta gatcccggct gtggcaccaa agcaagagcg
     2041 atcatcggaa tatttttttc gcgcaaaatt gtctcttcgt tttctacacc caacattgcc
     2101 tcaccgattt ccttcagctt gatatctctt ccatcgggaa agcttttgat aatcatgttg
     2161 ttaaattctt cgggtgtact caacctgcca atggtcttta tagtcaactc cgtttcatct
     2221 ccggaaatct taccgctggg tagctcaaca ttttgttttt gcagtgcatt ggttacatct
     2281 atggccgtta gatgaaagga tgataatttg acagggtcga gccaaagacg catagcatac
     2341 tttttctgtc cccaaatctg aagagatgaa actccgggga tggtttgcag tctcggaccc
     2401 aacacattct ccgcaaaatc actcaattcc atatgattgc gtgtattgct ctgtaccgtc
     2461 atggaaatta tggcatccga gttggcatct gctttggata ctatcggtac cccatccaga
     2521 tctttaggca actgccttat agcttgtgag actttgtccc ggacatcatt ggcagcactt
     2581 tccaaatcgg aggaaaggtc aaactctaca gtgatgttgg aacttccttg attggaggcc
     2641 gaagtaatgg tacggattcc ttgaataccg ttgatcgctt tctcgagcgg ttcagttatc
     2701 tgggattcaa tgatatcggc attcgccccg ggatagttgg ttcttacatt gatgatggga
     2761 gggtcgacag ccggaaattc tcttacaccc agtgttttga agccaatgag accaaatagg
     2821 aggatgatca ggttcatgac aatagccaga accggtcgat tgacgctgat ggtagaaaga
     2881 ctggccatgg ttattttact ttatccggtt tgacatccat gtcttcctta agcatgataa
     2941 tgcccgtaat gatcaggctg tcacctgcat tcagcccatt gcttacctga acggtcttat
     3001 catccctgcc taatatttcg atcttttttt cgactacttt tccgtttttg tagagataga
     3061 ctttctgacc tttaagtatt ggaacaactg ctgaagaagg gattctgaga acatcgctct
     3121 cttgggaaat attgatatct accttgacaa aatttccggg caagagattg gcgacatttg
     3181 gtgcaacacc tctgatgata acggatcgag tgttgagatc tatttgtgga tcaatggtct
     3241 gcacggttcc ggttagtgtt tttttacttg cctctgaata aaaattaaat ttctgaccga
     3301 ctttgaagtt ggcaaggtat ttttcaggtg cagtgaattc aattttgatt ggatccagtt
     3361 gttgaagtgt tgtaactact actgaaggac ttacatagct gcccaatgca atgttcctga
     3421 atccgacgat tccactgaat ggtgcacgta actcagtctt gtcaatcagg gtttgataat
     3481 aagcaatatc cgccttaaag ctcaatactt cattgtgggc gatatcataa tcctccttgc
     3541 tggttccctg aatagccagc aatttttcct gtcgtgcggc cttgtcttct gccagtttaa
     3601 gatttaacct ggatttgttg atttgcgcct ttaagtcggc atcgttcaat ttgaccagca
     3661 attggccggc cttgaccagt ccaccttctt taaaataaat accggacact tttccagccg
     3721 cttcaggaga aaggtccacc ttttcgtttg ccagaattgt tcctgtagat tgtagtgttc
     3781 cgttagcttt ttcagatttt aatacctgga tatctactcc aagcggcccg gatttctttc
     3841 cacctgccat aaatcccatt ttttgctctt cctgagcagg gaaaaagaga aatttggcta
     3901 caaataatgc ggcgacaatt gctgtaatgg ttattagaac gatgtattta cgcataaaaa
     3961 acgcttattt cttatagttt gatttcgttg aatccgacca tgaaactgac aaaattaaaa
     4021 aaataagttg accaaagaat tatctagact ttattgattg acatagctca accaaaacac
     4081 cgttggttga cttgggatgt agaaaacaaa tcaatttgtt atcagcaccg ggctttggtt
     4141 cttcattcag taatatgaat cccttttccc gcatctcctg catggattca aggatatcgt
     4201 ccacctcaaa agccaggtga tgtatgcctt gtcccttttt cgaaatatat ttggaaattg
     4261 cagtttcaac ttccggatct gagccaacca gttcgatttt gctttctcca agctgaaaga
     4321 acgatgtggt cacattttca ctatcaacat gttcttcctt atatgctttc cggccaaata
     4381 atttttcaaa aagttcgttt gcttctttga ggttacttac tgcgatacct acatgttcca
     4441 gacggatcat aattttaatt ttattctaat cttaatataa aaaaggctgc ctctaatgag
     4501 acagcctata ttttttcaga aaggtcctat tactcgaata ctgtaaatct ttgaatattg
     4561 acgccttcct tgttttcaat tttcaatagg taagtgccac tgcttaaaga actgacatca
     4621 aaactaagat tattggaacc gttaaagatg gtctgactgt tgttaagagt cagaactgtt
     4681 tttcctgtat aatccaaaat agtaacagtt gctgtcgatg gatttttagc gttgattctg
     4741 acattcaact gatcggcagt tggattcgga tatacactca cattctcaaa gctattgatt
     4801 cctttgacgg tattggttgc tccttgacca acgaagatat tatccaggta tcctgcgtta
     4861 ccccagttgg atgtaacagt agtcttgaat ataacatcat ccgtattggc caattcagaa
     4921 agagctacgg tatcagtcct ccattcagag gctgtaggta aaaacagggc cgtcttatca
     4981 ggagcggtct tcattgtatt tccggataag gttttgaact ttttgtaagt agcgccacaa
     5041 tcggttgaat aaaacatttc aaccttgtca ccatcgtatt taactccatt ttcaagataa
     5101 ccggtataag cccagtcata ggtatacaca ggattggatg gcatattgat cttatcgcta
     5161 ataaatgaca tagaagcgat tgttgatgcc ggattccact ggaagaagaa aaggattatt
     5221 gaattagctg atttgccata tccacctctg ttagccggag ggttagtcag attgggggtc
     5281 atagtaaaaa tatagtcaaa attcgcatca tcgaggaaca gtgttttacc taatgcacca
     5341 tcaccttcca tgtcataaga tttgggagcc ggagcaccgt gttcactcaa atatgtgaaa
     5401 gaataggttt ctgtaagatt gttaattaga ttaacatctt tagcgccatt gttgaatgaa
     5461 acaatccaca catcaatctg gttggtaccg ccgggaaccg gaatagaacc aaagcttata
     5521 ttttctgatt tgccaaattc gatattagta tcaaaactct tctctacctc ttctccgttg
     5581 aggctatagg caacagtaac ttgtgtgatt gcagacttat ccggattctg attggttatt
     5641 ttaataattg gttctgcagt atattctgca ggacactttt cagaacttga tttagaactg
     5701 ttcgcaacac ccggatcacc tacagttgta ccggcaggga aggaaaccgg ctcgctcaat
     5761 gcagcattca taacggatag gtcggttgtc ttttggatga aggccaaagc ctctatttgg
     5821 ttcagagcaa aaaagtaatt tttcaaggca acggtatagg taaatgtagc agactctcca
     5881 ctggcaaatg aagcaggaag cacattgtct ccaaatatag aacttgccgc ttttctgaat
     5941 acatgcgaga agtctttttc tccgtttgat cccggagcaa ccggccagtt tagcttagat
     6001 tccaacaaat atgcatgcag attataacca ctaatattaa atgtagtatc actattgttt
     6061 gttacgatta ctgttatatt tacagaatca atggtgttgt tgtaactatg agaaacctgg
     6121 atatctaaag gactgacctg agctgcggca tagttaaatg cagcttgatt gtatccgacc
     6181 ggtgcaccat aataggcaga attccagtta cctcctccat agttaccatt acccatataa
     6241 ccatctagga aggcagtcgg aacacctccc aagccgttat ctgacccgta aagtccgact
     6301 gtataatctt cataggtaga ccatttggct tgaacttcct ctttgttagt ttcaaacatg
     6361 gggtcgaaac cgggaaacca aacctgatat tttactgcta tgattttacc ttcattggcg
     6421 tcaataagag tattaaaagc agggttctgt gcagcacatg gaccgcagga ggcattggta
     6481 aactcttcaa ccaatccaag cttgcgtaag cctctgatta ttggttggtt tttcttcgtt
     6541 tcaattttaa ccagatgggt cttttcggtt tctccggatt tattaattgg agatacaaca
     6601 cattgaccaa cactggttga tacgttctta acagatcttt gtgcgcttac attcatggcg
     6661 caaaaaatca aaagaagtag tgtaaaatac ttactgttca taaatgaata tcttattttg
     6721 ttaaatgaaa gcgcaaaggt acgaattaaa ctactattga cagaatataa atttaattat
     6781 ttaagaaata tttatcattt gcgaaatgat gtccccaggg caacaaatat tatttcgatg
     6841 gagtctaatt tattcgtttg aacaatattt tattttgaat ttgtataaat aaactttgaa
     6901 gatcaaccta tgcccatgat atgctgatgt aattttttta agaactggtc aataaatctc
     6961 tttcgggaaa taattatgtt aaagaatcaa ggtctttttt cttcatagcg gccttttctg
     7021 ctgaatactc tcttccaaac aaccgataaa tcc
//
LOCUS       DAOQQN010000149         7012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4438_length_7012_cov_5.451488, whole genome shotgun sequence.
ACCESSION   DAOQQN010000149 DAOQQN010000000
VERSION     DAOQQN010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7012
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4438_length_7012_cov_5.451488"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..477)
                     /locus_tag="PLG24_06800"
     CDS             complement(46..477)
                     /locus_tag="PLG24_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HMZ24633.1"
                     /translation="MANYLDNIVAVSGLPRLYYMDGSRSNGLILKDMDNGTRQFVSAR
                     KHQFTPLRSIAIYTYSDSTPIEEVFKAISLLKGELKISEILKSDNKTLAVNFRKILPD
                     YDEDRVYPGDMKKVFKWFEFLDARNIITADSEVNTEKHEEE"
     gene            complement(494..2509)
                     /locus_tag="PLG24_06805"
     CDS             complement(494..2509)
                     /locus_tag="PLG24_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HMZ24634.1"
                     /translation="MEIVETSGILIETLPGADIPPVESTLIELYKKLVYPRLIEEKML
                     NLLRQGKISKWFSGIGQEAISVGVTAALDEDEYIFPMHRNLGVFTSRNMPLETLFSQW
                     QGTEGGYSKGRERSFHFGSVPHHIVGMISHLGPQLSYASGVALGHKLAGNRKVTAVFS
                     GEGGTSEGEFHEALNVAAVWSLPVIFVIENNGYGLSTPVNEQYRCERISDKGKGYGMR
                     AITIDGNDIVNVFNTVRILSEEIKADPHPVLLECVTFRMRGHEEASGIKYVPKELLEY
                     WSTQDPILRYEKYLLERNLMNENDFLSIRSKFKEEINEAVNNALSHSPIVPSTQTELD
                     DVFAPFEYNEISGGPVETELRMIDAISDGLNEAMKKHSNLILMGQDIAEYGGVFKITD
                     GFLERYGKDRVRNTPLCESAIIGTALGLSLKGYKAMVEMQFADFVTCGFNQIVNNLAK
                     LHYRWGQHADVVIRMPTGGGVGAGPFHSQSNEAWFTHVPGLKVAYPSNPYDAKGLLLT
                     AFEDTNPVLFFEHKALYRSQIRMVPEGYYNLPFGKAAIARKGEGMTIITYGMGVHWAL
                     KAADDHPDADIEVIDLRTLVPLDVDSILTSVKKTSKVIVLHEDTLFGGLGGEIAAIIS
                     EKAFEYLDAPVRRCAGLDTPIPFSIPLEKNFMADSRLGKIIEELKIY"
     gene            complement(2538..4979)
                     /locus_tag="PLG24_06810"
     CDS             complement(2538..4979)
                     /locus_tag="PLG24_06810"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HMZ24635.1"
                     /translation="MADNNFLKKLKSILASIRIRSNKIDIFLEKRPGLNFVLKAGISA
                     LLTGFICLFVLIFSARIGVFGGMPTDDELKEISNPEGTELFADDGVTLLGRYYIENRS
                     NITYDSLPENLIHALIATEDIRYYEHNGIDWKSWARVLFRTILMQESASGGGSTISQQ
                     LAKNLFKRRTYWRGTTLINKMREIIIAQRLESMYTKEQLLTLYFNTVSFGGNVYGIDI
                     ASKQYFKTSPRFLKTEEAATLVGMLKATTKYNPLNNIDLAKKRRNTVLNKMEKYQYIT
                     QKQLDSLSALDIKLNYVKETHTEGIATYFREFARLDLDKQLAHLKKNDGSSYNLYTDG
                     LKVYTSINIPMQNYAESAVADQMKELQELYYQQMRWIKLPWKDTAFLHKAIMNTDRYK
                     SMVTAGLSQHYIDSVFNVPYEMEVYSPQGPKTMKMSPKDSLIHYLQFLTAGFMAADPS
                     TGFIKAWVGGIDYKYFQYDHVKAKRHVGSTMKPIVYAAALEKGLDPCAYYDNRQKTYP
                     QFENWSPKNFEGGYGGEYSMAGALAKSLNCATVDIMLKTGIDTVVDFAKRIGVTSTIP
                     YEPSVSLGAVDISLYDMITAYSTIIDRGQKPALTYILKVVDSEGNVLIDNTTPEAQEK
                     VQVMNPENADIMRSMMQGVINHGTGGSIKWRYSITEDLAGKTGTSQDCSDGWFIASNP
                     KLIAGAWVGGEFPEIHLTGNFTGAFTALPIVGMFLRSVEKDKSLNDYVKEKFPELSQE
                     AKNRLNCPYSHYVADSSAIDSTGLDSLGNPIITDDDFHEQDDESPKVDTTKKKKFFEG
                     LFNRKEKKKVDNENN"
     gene            complement(5175..>7012)
                     /gene="rpsA"
                     /locus_tag="PLG24_06815"
     CDS             complement(5175..>7012)
                     /gene="rpsA"
                     /locus_tag="PLG24_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HMZ24636.1"
                     /translation="YEKTLTLIKDNEIVKGLVKSISSGDVVLDINYKSDGIVSLSEFR
                     DTPDLKVGDYVDVYVESQEDAKGQLVLSRKKAKLLIAWDTIVDSFNNGTIIKGTVISK
                     TKGGLIVDAYGLETFLPGSQIDIKPIIDYDAYVGKTMEFKVVKINETIKNAVVSHKAL
                     IESDIAEQREHIIAGLEKGQVLEGVVKNITDFGAFMDLGGVDGLLYITDISWGRINHP
                     EEVLQMNDKINVVVLDFDDNKKRISLGLKQLTPHPWDVLAMDISEGSIVKGKIVNVED
                     YGAFLEISPGVEGLIHVSEVSWSNQPVVARDFFKLDQEFEAKVVTIDRDERKMSLSLK
                     QLQEDPWTEIENKYPVNSRHTGTVKNLTPYGVFVEIDNGIGGMVHISDLSWTKRFSHP
                     SEFTKVGDQIEVVILEIDKANRKLSLGHKQIEENPWDAFEAVFPVGSYHEATVIRRDD
                     KGATVQLPYGLEAFAPIKHLKKEDGSTAEVEESLTVKVIEFNRDDKRILVSHSRYLED
                     IKKEADNLIKRDKESADNETKSAIKTQQAKLEKTTLGDIDAFSQLKQQFDEAGEKKAE
                     EAKEELALKAKKSTKATKAKEVVAEETAATEGGDTPEDSEKGEDA"
BASE COUNT         2075 a         1574 c         1189 g         2174 t
ORIGIN      
        1 cagcattata ggcaggcaga actgctacta ctttgaggtt gttaatcatt cctcttcgtg
       61 cttttcagta ttaacctcag agtcggcagt tatgatattt cttgcatcaa ggaattcaaa
      121 ccatttaaag acctttttca tatcacccgg gtaaacccgg tcttcgtcat aatccggtag
      181 gatctttctg aaattcacgg ccagggtttt attatctgat ttcagaattt ctgaaatttt
      241 taattcacct tttaaaagcg agattgcctt aaaaacttct tcaatcgggg tggaatcact
      301 atatgtataa atagcaatcg acctaagggg cgtaaattgg tgctttcttg ctgaaacaaa
      361 ttgccgggta ccattatcca tatctttcaa tatcaatccg ttgcttctgc taccatccat
      421 gtaataaagc ctgggtaaac cacttactgc cacaatgtta tccaaataat tagccattat
      481 cttttatcct tatttaatat attttcaatt cttcaataat cttacctaat ctggaatcag
      541 ccataaaatt cttctctaaa ggaatagaaa aaggaatagg tgtatccagg cccgcgcacc
      601 tgcgtacagg cgcatccaga tattcaaaag ctttttcact aatgatagct gcgatctccc
      661 ctccaagacc accaaatagc gtatcttcat gcaatacaat aaccttactt gttttcttga
      721 cggatgtcaa tattgaatca acatccaaag gtaccaaggt cctgaggtcg ataacttcaa
      781 tatcagcatc aggatgatca tcagctgctt taagtgccca atgaacaccc ataccatacg
      841 taatgattgt catgccttca ccctttcttg caatggcagc ttttccaaat ggcaagttgt
      901 aataaccttc cggaaccatc ctgatttgag atcggtataa tgccttatgt tcaaagaata
      961 atacagggtt tgtatcctca aatgcagtca gtaaaagacc cttggcatca taaggattgg
     1021 atggataggc caccttaagg cccggtacat gggtaaacca tgcttcattg ctttgggaat
     1081 gaaaaggtcc ggctcctact ccaccgcctg taggcattct gataaccaca tcggcatgtt
     1141 gaccccaccg ataatggagt ttggcaagat tattaacaat ctggttgaac ccgcaagtaa
     1201 caaaatcagc aaactgcatc tcaaccatag ctttataacc cttaagacta aggcctaaag
     1261 ctgttccgat tattgcgctt tcacaaagag gagtatttct gactctatcc ttcccatacc
     1321 tttctaaaaa tccatctgta atcttaaaga caccaccata ctcagcaata tcctggccca
     1381 ttaaaatcag attggaatgt tttttcattg cctcattcaa tccatctgag atcgcatcaa
     1441 tcatcctcaa ttcagtctca acaggacctc cactgatttc attgtattca aagggagcaa
     1501 aaacatcgtc cagctcagtt tgagtggatg gcacaatggg tgagtgactg agcgcattat
     1561 ttacagcttc attgatttct tccttaaatt tggatctaat tgaaaggaaa tcattttcat
     1621 tcatcagatt cctttccaaa agatattttt catatctcag gatgggatcc tgtgttgacc
     1681 aatattccaa caattccttt ggaacatatt tgattccgga agcttcttca tgtccccgca
     1741 ttctgaaagt aacacattcc aaaagaaccg gatgcggatc agctttgatt tcttccgaaa
     1801 gtattctgac cgtattaaat acatttacaa tatcattacc gtcaattgta attgcacgca
     1861 tgccatatcc tttccctttg tcgctgatgc gttcacatct atattgttca ttgaccggag
     1921 tggaaagtcc atacccatta ttttcaataa cgaaaattac cggaagactc caaacggctg
     1981 ctacgttcaa tgcttcatga aattctccct cacttgttcc accctcacct gaaaatacag
     2041 cagtaacttt tctatttcca gcaagtttat ggcccaaagc gactccggaa gcataagata
     2101 attgcgggcc taagtgagag atcataccaa caatgtgatg agggacggaa ccaaaatgga
     2161 aagaacgttc gcgccctttg gaatatcctc cttcagttcc ttgccattga ctgaacaggg
     2221 tttcaagagg catatttctt gatgtgaata ctccgagatt acggtgcatt ggaaaaatat
     2281 actcatcttc atccaaggca gcggttacgc caacagaaat agcttcctgg ccaattccag
     2341 aaaaccattt gcttatttta ccctgccgga gaaggttaag cattttttct tcaatcaacc
     2401 tgggataaac caatttttta tacaattcaa tcaaagttga ttcaacagga ggtatatcag
     2461 ctcccggcaa agtttcaatc aatatcccag acgtttcgac aatttccatt tcgattcaat
     2521 ttcttgagaa acaaacatca gttgttctcg ttatcaacct ttttcttttc ttttctatta
     2581 aaaagccctt cgaaaaactt cttcttctta gtggtatcta cttttggcga ctcatcatcc
     2641 tgctcatgga aatcatcatc ggttattatc ggattaccga gcgagtcaag tccggtagaa
     2701 tcgatagccg aggagtcagc gacatagtga gaatagggac aattcaaacg attttttgct
     2761 tcctgtgata attcaggaaa tttctccttg acatagtcat tcaaggactt atctttttca
     2821 acacttctca aaaacattcc aactatcggt aaggcggtaa aagcaccggt aaaattaccc
     2881 gtaagatgta tttcaggaaa ttcaccacct acccatgccc cggcaatcaa tttcggattt
     2941 gacgcaataa accaaccatc actacagtcc tgacttgttc cggtttttcc ggcaaggtct
     3001 tctgtgatgg aatatctcca cttgatagaa ccaccagtac catgattaat aaccccctgc
     3061 atcatggatc gcatgatatc cgcattttca ggattcatta cctgtacttt ttcctgggct
     3121 tccggtgttg tattgtcaat caatacattt ccttcagaat caacaacctt caagatataa
     3181 gtcagagctg gcttttgccc tctatcaatg atagtagaat aggcagttat catgtcatat
     3241 aaagaaatat caacagctcc taagctaaca gatggttcat aaggaatagt cgatgttacg
     3301 ccaattcttt ttgcaaaatc aaccacggtg tcaataccgg ttttaagcat aatatccact
     3361 gttgcacaat tgagtgattt ggccaaagcg cctgccatgg aatattcgcc accatatccg
     3421 ccttcaaaat tcttaggaga ccaattctca aactgaggat acgtcttttg tcgattatcg
     3481 taatacgcgc aagggtcaag tcctttttcc aaagcagctg catacacaat cggcttcatg
     3541 gtagaaccga cgtgtcgctt agctttcaca tgatcgtatt gaaaatactt gtaatcaatg
     3601 ccccctaccc aagccttgat aaaaccggta gaagggtcgg cggccataaa tcccgccgtc
     3661 agaaactgaa gataatggat cagagaatcc ttagggctca ttttcattgt ctttggaccc
     3721 tgcggactat aaacttccat ttcatatgga acattaaata cactgtcaat gtaatgttgt
     3781 gacaaaccgg ctgtaaccat tgatttatac cggtcggtat tcatgattgc tttatggaga
     3841 aaagcggtgt ctttccaagg tagtttaatc cagcgcattt gttgataata tagctcctga
     3901 agttctttca tttgatcggc cactgcagat tcagcataat tctgcattgg aatattaatt
     3961 gaagtataaa ctttcaagcc gtccgtatat agattgtagc tgcttccgtc attctttttc
     4021 aaatgagcca attgtttatc aagatccaat cttgcaaatt cgcgaaaata agttgcaata
     4081 ccttccgtat gtgtctcctt gacataattc aacttaatat ccagtgcaga caatgaatcc
     4141 agttgttttt gggtgatata ttgatacttt tccattttgt ttaaaacagt atttcgcctt
     4201 ttcttggcaa gatcaatatt attgagggga ttatacttgg tggtggcttt aagcatacca
     4261 accaaagtag ctgcctcctc cgtctttaaa aatctgggag aagttttaaa gtattgcttt
     4321 gaagcaatgt caataccgta gacattacca ccaaaggaaa cagtattaaa ataaagcgtc
     4381 agcaattgtt ctttagtgta catactctcc aatcgttgag cgataataat ctcacgcatt
     4441 ttattgataa gggtagtgcc acgccaataa gtccttcttt taaataaatt tttagccaat
     4501 tgctgactaa ttgtgctacc ccctcctgat gcactttcct gcattaaaat tgttctgaac
     4561 aacactcgcg cccaggattt ccaatcaata ccattgtgtt catagtatct gatatcttcc
     4621 gtagctatca aggcatgaat caaattctcc ggcaaagaat cgtaagtgat attagatctg
     4681 ttttcaatgt aatacctacc taaaagcgta acaccatcat ctgcaaataa ttcagttcct
     4741 tcaggattac taatttcttt cagttcgtcg tctgtaggca ttccaccaaa aacacctatt
     4801 cgcgctgaga agattaaaac gaacaagcaa ataaagcctg tgagaagtgc actgataccg
     4861 gcttttaaaa caaaatttaa accgggcctt ttttcaagga aaatatcaat tttattactc
     4921 ctaattcgta ttgatgcgag tatactcttc aactttttca agaaattatt gtcagccatt
     4981 taggtcctta agcgattaag cgataaaaat acaacgattc aaagatagat aacgaaaata
     5041 tatcagggat gttgaaataa acttctcttt ctcacattga caaacaatta cataattgaa
     5101 aagccgtgct tgaaaagaat ttcaaaacac ggcttttgat tatttagctg aatctaaatg
     5161 attttcacaa aaaatcaagc atcttcacct ttctcagaat cttcgggcgt atcacctccc
     5221 tctgtagcag cagtttcttc agcaacgact tcttttgctt tagttgcctt agtagatttc
     5281 ttagccttaa gagctaactc ttccttagct tcttctgctt tcttttcacc ggcttcgtca
     5341 aactgttgct ttaactgaga aaaagcatca atatcaccca gggtcgtttt ctctaatttg
     5401 gcttgttgcg tcttaattgc tgactttgtt tcattatcag cagattcttt gtcacgcttg
     5461 atcaaattat cagcttcctt tttgatatcc tccagataac ggctatgaga aacaaggata
     5521 cgcttatcgt ctctattgaa ttcaattacc ttgacagtaa gtgattcctc aacttctgca
     5581 gtagatccat cttctttctt caggtgcttg ataggagcaa aagcttccaa tccataaggc
     5641 agctgaactg ttgcaccttt atcgtccctt ctgattacgg ttgcttcatg ataagaccct
     5701 acagggaata cagcttcaaa tgcatcccaa ggattttctt ctatctgctt atgtcccaaa
     5761 gacaactttc tattagcctt atcaatttcg aggatgacaa cctcaatctg atcaccaact
     5821 ttagtaaatt ctgaaggatg agagaatcgt tttgtccatg aaaggtcaga aatatggacc
     5881 attcctccaa taccattgtc aatctcaacg aaaacgccat atggagttaa attcttcaca
     5941 gtaccggtat gtcggctatt gactggatac ttgttttcaa tttctgtcca aggatcttcc
     6001 tgaagttgtt tcaagctcag ggacattttc ctttcatctc tgtcgatagt aactaccttt
     6061 gcttcaaact cctgatccaa cttgaagaaa tcacgcgcaa ctaccggctg gttactccag
     6121 gaaacttcag aaacgtgaat caatccttca acgcccgggc ttatttcaag gaatgcacca
     6181 tagtcttcga cattgacgat ctttccttta acaatagagc cttctgaaat gtccatagcc
     6241 agaacgtccc aaggatgagg agtcaactgc ttcagaccga gagagatacg ttttttatta
     6301 tcatcaaagt ccaaaacaac gacattaatt ttatcattca tttggagaac ctcttcagga
     6361 tggttgatcc taccccatga aatatcagtg atataaagga gaccatctac accaccaaga
     6421 tccatgaatg caccaaaatc ggtgatattc ttaacgacac cttcaagaac ctgtcctttt
     6481 tcaagaccgg caataatgtg ttcacgctgt tcagcgatat cgctctcaat aagtgcctta
     6541 tgtgaaacaa ccgcgttctt gatagtctcg ttaattttaa ctaccttgaa ttccatggtc
     6601 ttaccaacat aagcatcata gtcaataata ggcttgatgt caatctgaga gccgggcagg
     6661 aaggtctcaa ggccataggc atcaacaata agtccaccct tggtcttgga aataacagta
     6721 cctttgatga tagtaccatt gttaaaggaa tcaacaatag tatcccaggc aatcaataat
     6781 ttagctttct ttctggaaag caccaactgc ccttttgcat cttcctgact ttctacatag
     6841 acatctacat aatcaccaac cttaaggtca ggtgtgtcac ggaattccga aagagaaaca
     6901 ataccatctg atttgtaatt aatatccaat acgacatctc cactgctaat agatttcaca
     6961 agacctttta ctatctcatt atctttgata agggtcagtg ttttttcgta at
//
LOCUS       DAOQQN010000150         6962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4495_length_6962_cov_5.545823, whole genome shotgun sequence.
ACCESSION   DAOQQN010000150 DAOQQN010000000
VERSION     DAOQQN010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4495_length_6962_cov_5.545823"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLG24_06820"
     CDS             <1..180
                     /locus_tag="PLG24_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24637.1"
                     /translation="TQMLITFHSVPLGIFVTPGCPIINHLPTSCLPPPPYPFSCAYNT
                     FCLNETHGQRDFYKD"
     gene            complement(233..1810)
                     /locus_tag="PLG24_06825"
     CDS             complement(233..1810)
                     /locus_tag="PLG24_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HMZ24638.1"
                     /translation="MIQLNIDNSFTRSLPADPDTTNKVRQVRNAAYSFVNPMHFSKPE
                     LIHVSPEAATLIGLTSADIKSSYFLEIFSGQRIYPNTKPYAMAYAGHQFGNWAGQLGD
                     GRAINLFETLHEGRRIATQLKGAGSTPYSRHADGLAVLRSSIREHLCSEAMHHLGVPT
                     TRSLSVIKTGDQVIRDMFYDGHPAPEPGAVVCRLSPTFLRFGNFEFFAANGDEKNLRK
                     LLEYTIREFYPSIDSTSKSSGLDFFREICRKTIALIIEWQRVGFVHGVMNTDNMSAIG
                     LTIDYGPYGWIDNYDEDWTPNTTDAELRRYRFGRQDSVALWNLYQLANALHTIIGHAD
                     ALRDILDEAEKLIPIGYYSMMIKKLGLRDLDEESIDLVDRVRKLLQQHETDMTIFFRK
                     LSTLDISAFQPDKTGITSWMGEAFYSSPDNNSAVLTDWEKWFVDYIARLKRENIPVHQ
                     RHLDMKSVNPKYVLRNYMAQMAIDAAYKGDYTLVDELYEMLRKPYEDQPEYDRWFVRR
                     PDWARTKAGCSMLSCSS"
     gene            complement(1807..2352)
                     /gene="msrA"
                     /locus_tag="PLG24_06830"
     CDS             complement(1807..2352)
                     /gene="msrA"
                     /locus_tag="PLG24_06830"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HMZ24639.1"
                     /translation="MSNIQKATLGGGCFWCVEAVMQQLRGVESVVSGYTGGKFPAPTY
                     REVCSGLTGHAEVVQVTFDADILSYKDLLMIFMTTHDPTTLNRQGADKGTQYRSVIYY
                     HNDGQKAEIDKVMSELKHIYEDEIVTEISPLPPFFPAEDYHQNYFKENPVAPYCRIVI
                     APKVDKLRRDYTAYLKQETNQ"
     gene            complement(2591..3925)
                     /locus_tag="PLG24_06835"
     CDS             complement(2591..3925)
                     /locus_tag="PLG24_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HMZ24640.1"
                     /translation="MYKVISIKSRWLIAFILLIAWSFTSNGQTLYELQYNYDTEFGLE
                     RYRALLVIYEDGTGFIRIAFKDLESGSDNVVDFDVDEFYDTDQKNNLDDLYYITSNPI
                     QIKGDISYPPDHFVLSKNKNTGYYEPSMVISFDEGEEEIGNFESVTLLKESDLTQDYI
                     SIYFTPEDEIYQNLFINEVKPVPPFMKNIQLHLILVANTLDPIIGKTCVVDRDNILKL
                     FDDVTHFLEIGYDKQVIEGQNFSRKNVDIAISKLNPGPNDIVVFYYTGHGYNPETGNK
                     KYPNLDLRERESDDIGGLNTLNIEDIYRLVQGKNARLNLVLSDCCNNDIFSTNSISSE
                     AASLRSSSVGWNKNFCSDLFLNTKKSVLMTGASKGQLSAGNAIDGGFFTFNLRDNITK
                     SVGKLSNKFSANWEHITKETQKLTMKKAEDSKCRMPDKTVKKCNQTPDFTIK"
     gene            4356..5753
                     /locus_tag="PLG24_06840"
     CDS             4356..5753
                     /locus_tag="PLG24_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ24641.1"
                     /translation="MIEYLKKYTDSIVIIICLIVLSGLCCLGQDYNKKSLLDSLQSAT
                     DPETRVRLLWNLADELQRYDLDSSLILSHQAIVIGRAINYKEGLSRSYGIIGNVYSRM
                     NDFPKALEYYFSKQKIDEQSGNARNLASTLINIGVVFSLQTDFKEALAYFEKADSISH
                     KNNLTEMYYHLDINLGNVYEKLKQADRAAIFYNKALNSALMKGDSLNMALAHIGLANV
                     YSMKGDEFKALNNYDLAIPVIKESKDMEMYSEALLGKAKIYFEFGDNVNALTDALKSY
                     REAKAIESFLKMYESSDFISGLYQKQQAYDSAFAYLKLSKMYGDSLNNLSRLKNIQAL
                     TVNEKIRQNEIAEYKKLEHERAKFRLQVILVTVFIPMLFLVSIILSKRRIKHSVIKFL
                     GVVSLMFAFEFITLLLHPLVENITGHNLFIEVFIFVIIGFILVPAHHRIEKTIIRILI
                     NNRIQLELSKKEPDQ"
     gene            complement(5799..5993)
                     /locus_tag="PLG24_06845"
     CDS             complement(5799..5993)
                     /locus_tag="PLG24_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24642.1"
                     /translation="MRLLTIFLVFAGMSMFIACKQDNASTEPTEAEATTEVKTDAEPM
                     ADTTATDTMINETKPVSRPN"
     gene            complement(6027..>6962)
                     /locus_tag="PLG24_06850"
     CDS             complement(6027..>6962)
                     /locus_tag="PLG24_06850"
                     /inference="COORDINATES: protein motif:HMM:NF033578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HMZ24643.1"
                     /translation="NYYQYFCGECLFVPGIPCQPSELVHFRKRIGERGIELILKESIR
                     VNGKDSDDDNVNIDTTVQEKNITFPTDGKLYKKMIKKCQKIAAQEGLPVRQTYTHTLK
                     KLARDQRFRNHPKNKAKARKADKKIKTIAGRLVRELERNLPPNSICQDLLARFQQILA
                     QTKDSKDKIYSIHEPDVCCISKGKEHKKYEFGNKASFAKTDTGVIVGAMGFRNEYDGH
                     TLPAVIEQIQRMTNKIPKVAKVDRGYRGKIEIEGMKILIPGTPKKKESYYKRNKLSKA
                     HKKRAGIEPVIGHINADHRLKLNNTINKKSNIFGN"
BASE COUNT         1894 a         1363 c         1383 g         2322 t
ORIGIN      
        1 acacaaatgc tgattacttt tcattcggtg cctttgggaa tcttcgtcac ccctggttgc
       61 ccgattatta atcatctacc tacttcgtgt ttgccccccc ctccataccc tttctcttgc
      121 gcctacaaca cattttgcct caacgaaacc catggccaac gagattttta caaggattga
      181 atttcaagtc atccggacaa cttcatacgc ctgattaatt ctctgcccga ttttaagatg
      241 aacaggacag catggagcag ccggcctttg ttcgtgccca atcaggcctt ctgacaaacc
      301 atctgtcata ttccggttgg tcttcgtaag gctttcgtaa catctcatac aattcatcta
      361 ccaaggtata atccccttta taagcagcat caatggccat ctgagccata taattgcgca
      421 atacatattt tggattgacc gacttcatgt ctaaatgccg ttgatgaacc ggaatatttt
      481 cacgttttaa tctggctata taatctacaa accatttctc ccagtctgta agtacagcgc
      541 tattattatc aggagatgaa tagaacgctt cacccatcca gctagtaatt cctgttttat
      601 ccggttgaaa ggctgatata tccaatgtgg acagtttgcg aaaaaatata gtcatatcgg
      661 tttcatgttg ttgtaacaac tttctcactc tgtccaccaa gtcaatactt tcctcgtcca
      721 gatctctaag gccgagcttt ttgatcatca tcgaataata acctatggga atcagttttt
      781 cagcttcgtc caaaatgtcg cgcagcgcat ctgcatggcc gatgattgtg tggagcgcat
      841 tggctaattg gtatagattc catagtgcga cactatcctg tcggccaaat cggtaacgac
      901 gaagttcggc atcggttgta ttgggtgtcc aatcttcatc gtaattatcg atccaaccat
      961 atgggccata gtcaatagtc agaccgattg ctgacatgtt gtcagtattc atgaccccat
     1021 gcacgaagcc tactcgttgc cattcgatga ttaatgcaat agtcttacgg caaatctccc
     1081 ggaagaaatc aaggcctgag gatttggagg tagaatcaat agatggataa aattcgcgaa
     1141 tagtatattc cagcaacttt cgtagatttt tttcatctcc gtttgctgca aaaaattcaa
     1201 aattgccgaa acgcaggaat gttggactta gtctgcacac cactgcaccc ggctccggtg
     1261 ccggatggcc atcataaaac atatcgcgga tgacttgatc gcctgttttg atgacgctca
     1321 aagagcgagt cgtcgggaca ccaagatgat gcatggcttc gctgcaaaga tgctctcgta
     1381 tggaactcct gagcacggca aggccatcgg catgccttga atagggtgtg ctgcctgcac
     1441 ctttcaactg agtcgcgatg cgcctgcctt catgtagggt ctcaaatagg ttgatggcac
     1501 ggccgtctcc caattgaccg gcccaattac cgaactgatg acctgcatag gccatggcat
     1561 atggctttgt attgggataa atcctttgcc cactgaagat ttcgagaaaa tagcttgatt
     1621 tgatatctgc tgatgttagt ccgatgagtg tggcagcttc cgggctgaca tgaataagct
     1681 cgggtttgga aaaatgcatg ggattaacaa atgagtatgc ggcattacgt acctgacgta
     1741 ccttgttggt agtgtccggg tctgccggta gcgatcgggt gaatgaattg tctatattga
     1801 gttgtatcat tgattagtct cctgttttaa atatgccgta taatctctac gcaacttgtc
     1861 taccttaggg gcaattacaa tacggcaata gggtgcgacc gggttttctt tgaaataatt
     1921 ttgatgatag tcttcagccg ggaaaaatgg tgggagaggg ctgatctccg ttacgatctc
     1981 atcctcataa atgtgtttta attcagacat taccttatct atctccgcct tttgcccatc
     2041 gttatggtaa taaatgacgg aacggtactg tgtgcctttg tcggctccct gcctgttgag
     2101 tgtcgtggga tcgtgagttg tcatgaagat catcagcaaa tccttataag acaggatgtc
     2161 ggcatcaaag gttacttgca ccacctcagc atgtccggtc aatcctgaac atacttcccg
     2221 gtaagtaggt gcgggaaact tgcctccggt atagccacta accacactct ccactccgcg
     2281 aagctgttgc atcaccgcct ccacacacca gaagcatcct cctcccaagg tcgctttttg
     2341 aatattagac atggttgtta ttgtttcttt agttttgaaa gacacattga tttatgtatc
     2401 ttttttatat taatgcctta ggttaataga atgtttaata gggttgactt cacggaaagt
     2461 tcaagttatt cggaaatttc tttttattac tgttaaatcg taaaaggata tctaatgtag
     2521 tttcttcata atgataaaaa tggtgtagtg tgtcggacat ttctagttgc taccttaatg
     2581 gttggcctta ttatttaatg gtaaagtccg gagtctgatt acactttttg actgtcttat
     2641 caggcattct gcatttgctg tcttcagctt ttttcatagt cagtttttga gtttctttcg
     2701 tgatatgctc ccagtttgca gagaatttgt ttgataattt tccaactgat ttggttatat
     2761 tgtcacgtaa gttgaaggtg aagaaaccgc catcgattgc attaccggct gaaagttgtc
     2821 ccttggatgc gcctgtcata agtacagatt tttttgtatt caggaaaaga tcggagcaaa
     2881 agtttttatt ccatcccact gaagatgatc gcagacttgc tgcttcagaa cttattgaat
     2941 tagtagagaa aatatcatta ttacaacaat cactcaagac taaattaagc cttgcatttt
     3001 ttccttgaac aagtcgatag atgtcttcaa tgttgagtgt atttaacccc ccgatatcat
     3061 ccgattctct ttctctaagg tcaaggtttg gatatttttt attgcccgtc tctgggttgt
     3121 acccgtggcc tgtataatag aaaacaacaa tatcattcgg tcccggattt aattttgaaa
     3181 ttgcgatatc gacattcttt ctggaaaaat tctgaccctc tatgacttgt ttatcatatc
     3241 ctatttccaa aaaatgagta acgtcatcaa atagcttaag tatattatcg cgatctacaa
     3301 cacatgtttt gcctatgatg gggtcaaggg tgttggctac aaggatcagg tggagctgaa
     3361 tatttttcat gaagggaggc accggtttga cctcatttat aaaaagattt tgatagatct
     3421 cgtcttcggg ggtaaaatat atggaaatat aatcctgagt aaggtcagat tcttttaata
     3481 atgttacact ttcaaagttt ccgatttcct cttctccttc atcaaaagaa attaccatgg
     3541 acggttcata atagccggta tttttgtttt ttgagagtac aaaatgatca ggcggataag
     3601 aaatatcgcc tttgatctgg atgggattgg atgttatata atacaaatca tcgagattgt
     3661 tcttttgatc ggtatcgtaa aattcatcga catcaaagtc taccacattg tcacttccgg
     3721 attccaaatc cttgaatgca attcgaataa accccgtgcc atcttcataa attactagca
     3781 aagccctgta tcgttcaaga ccgaattctg tatcgtaatt gtattgtagc tcatagaggg
     3841 tttgtccatt ggatgtaaat gaccatgcaa tcaacagtat gaatgcaatc aaccatcttg
     3901 attttattga aattacttta tacatatcct tgtctttgaa gtgtaaatat acggaatcgc
     3961 aaagattagc aggattattt taatcaataa ttcagtgttt gtagaataat gttttgtatt
     4021 attggcttat ggtaaaatat gtataattga caattaaaat gatttgattt ttgatgcctt
     4081 ggtgtgtatg aaggatgtgg ttttgataat tttttaaggg attcagggag atctgtttac
     4141 catattttta cggcatcatc ttttttccac agggatttat caacattcac tgtgatatcg
     4201 acaaacaatg cttcttcggt agacaagttg aagagttggg ctatttctta tttaagcatg
     4261 gcagtacgta attcattcag gtatagagat atttggagaa taggttaact ttagatttta
     4321 ataatacagc cgcactaact acaaatacaa atgatatgat cgagtattta aagaaatata
     4381 ctgattctat agttattatc atatgcttga ttgttttatc aggcttgtgt tgcttaggtc
     4441 aggactacaa taagaaaagt ttgcttgact cgctgcaatc cgccacggat cctgagacaa
     4501 gggtgaggtt actatggaat ctggctgatg aattacaacg ctatgatctg gattcttcgt
     4561 tgatactcag ccatcaggca attgttatcg gacgggctat aaattataag gaaggtcttt
     4621 ctcgatccta tggcattatc ggcaatgtat atagccgcat gaatgatttt ccgaaagcct
     4681 tggaatatta tttcagtaag cagaaaatag acgaacagag tggaaatgcc cggaatttgg
     4741 caagcacttt gattaatatc ggtgtggttt tttctctgca gaccgatttc aaggaggcat
     4801 tggcttactt cgaaaaggct gattccattt cccataaaaa caacctgact gaaatgtatt
     4861 accatctgga catcaatttg ggaaatgttt atgagaagtt gaaacaggca gaccgtgctg
     4921 ctatttttta caacaaggca ttgaatagtg cattgatgaa aggagatagc ttgaatatgg
     4981 cattggcaca cattgggttg gctaatgtgt attcaatgaa aggtgatgaa tttaaggcgt
     5041 tgaataatta tgatctggct attcctgtca tcaaggagag taaagacatg gagatgtatt
     5101 cagaagcttt attgggaaag gcaaagatat attttgaatt tggcgacaat gtcaatgcac
     5161 ttacagacgc actgaagtca taccgggagg caaaagctat cgaatcattt ttgaaaatgt
     5221 atgaaagcag tgattttatt tcaggcttat atcaaaaaca gcaagcgtat gatagtgctt
     5281 ttgcttactt gaagctttca aaaatgtatg gggattcctt aaataatcta tccaggctga
     5341 aaaatatcca ggcattgacg gtcaacgaga agattcgtca aaatgaaatt gcagaatata
     5401 aaaaacttga acatgagagg gctaaatttc gccttcaggt gattctggta actgttttta
     5461 ttccaatgct gttcctggtt agcattatac tgagtaagcg gaggattaaa cattctgtga
     5521 ttaaatttct tggagtagtg tccctgatgt tcgcctttga atttattacc ttgcttttac
     5581 atccattggt ggaaaatata actggccaca atctgtttat tgaagtattt atttttgtaa
     5641 ttattggttt tattcttgtc cctgcacatc atcggatcga aaagacgatc atcaggatac
     5701 tgattaataa ccgaattcaa cttgaattat caaaaaaaga gcccgaccaa tagggccggg
     5761 ccttttattt atgttcaaag attaatcttg tctgctgatt agtttggcct ggaaacaggc
     5821 ttggtttcgt tgatcatagt atctgtggcc gtggtgtctg ccataggctc agcatctgtt
     5881 tttacttcag tagtagcttc agcctcagtt ggttctgtag aggcattatc ttgcttacat
     5941 gcaatgaaca ttgacatgcc cgcaaaaact aaaaaaatgg ttaataaacg cataattccg
     6001 gttttagtta gttaaaaaat tgtttatcaa ttaccaaata tattgctttt tttattaatt
     6061 gtattgttaa gtttcaatct gtgatccgca ttgatgtgtc cgataacggg ctctattcct
     6121 gctctctttt tatgcgcctt gctcaattta tttcttttgt agtaactttc ttttttcttt
     6181 ggagtgcctg gaattaagat tttcatccct tctatttcta ttttaccccg gtatcctctg
     6241 tctactttgg ctactttagg tattttgttt gtcattcgtt ggatttgttc tattacagct
     6301 ggtagggtgt gtccatcgta ctcatttcga aaacccatcg ctcccactat tacacctgtg
     6361 tcagtctttg caaaagaggc tttattccca aattcatact tcttatgttc ctttcccttt
     6421 gaaatacaac atacatcagg ctcgtgaatg gaataaattt tatcctttga atctttcgtc
     6481 tgtgccagta tctgttgaaa ccgtgccaat aagtcttggc aaatcgaatt cggtggaaga
     6541 tttcgctcta attctcttac caatcttccc gcaatcgtct ttatcttctt atctgctttt
     6601 cttgcttttg ctttgttctt cggatgattt ctgaatcgtt ggtcccttgc cagttttttc
     6661 aatgtatgtg tataagtctg tctcaccggt aagccttcct gtgccgctat cttctgacat
     6721 ttcttaatca tctttttgta aagcttccca tccgttggaa atgtgatatt tttctcttga
     6781 accgtggtgt caatatttac attgtcatca tcagaatcct ttccatttac ccggatactc
     6841 tctttcaata tcaactcgat tccacgctct cctatccttt tcctaaaatg taccaactct
     6901 gatggctgac acggaatgcc gggaacgaaa agacattctc cacaaaaata ttggtaataa
     6961 tt
//
LOCUS       DAOQQN010000151         6927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4523_length_6927_cov_5.026775, whole genome shotgun sequence.
ACCESSION   DAOQQN010000151 DAOQQN010000000
VERSION     DAOQQN010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4523_length_6927_cov_5.026775"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="PLG24_06855"
     CDS             complement(<1..1177)
                     /locus_tag="PLG24_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568727.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HMZ24644.1"
                     /translation="MAKETTPYRPTNHIRIVTSGSLFDGHDAAINIMRRIMQASGAEI
                     IHLGHNRSADEIVNAAIQEDAQAIAITSYQGGHNEFFKYIYDLLKERHAGHIKVFGGG
                     GGTILPEEIKQLEAYGIARIYSPDDGRALGLQGMIDDVLRLCDFPVGDQLNINTGQLN
                     SHNPHVVARIISAAENYPDQHRELLGKLNEKAEASAAPVLGITGTGGAGKSSLIDELI
                     RRFLLEYKDKTMGIISVDPSKRKTGGALLGDRIRMNSINKPRIYMRSMATRQSNQALS
                     AQINAALNILKAAGFDLIILESSGIGQSDTEITQYADVSLYVMTPEFGAASQLEKIGM
                     LDYADAVAINKFDKRGAADALRDVKKQYQRNHQLFDQSPDKMPVFGTIASQFNDPGTN
                     "
     gene            complement(1462..2118)
                     /locus_tag="PLG24_06860"
     CDS             complement(1462..2118)
                     /locus_tag="PLG24_06860"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMZ24645.1"
                     /translation="MNIKYIVISVVLFTLSGFTAFAQRGYHGPIQLSGVVVTQEKGQP
                     VRMPYVTIAVEGTSRGTYTNWDGFFSLVVEKGETIVFTYVGYKTIRYVVPDTLSTNRY
                     SIFQIMTFDNILLPETVIYPWPDKDNYHAEFLAMNINNDITDRVQENLDRRVLASLYD
                     KVPVDGKESTQSFMRQNASTYYYQGQTRPIQLMNVMAWKQFIEAWKAGKFKKKQDDGK
                     "
     gene            complement(2537..3826)
                     /gene="eno"
                     /locus_tag="PLG24_06865"
     CDS             complement(2537..3826)
                     /gene="eno"
                     /locus_tag="PLG24_06865"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HMZ24646.1"
                     /translation="MSAIIDIRSREILDSRGNPTVEVEVLTEDGAVGRAAVPSGASTG
                     KHEAVELRDNDNKRFLGKGVLKACKHVDDEIRDEVVGMDVFLQREIDQVMIEADGTKN
                     KSKFGANALLGVSMAVAKAAAKEAGQPLYRYIGGVNACTLPVPMMNILNGGSHADNSI
                     DFQEFMIMPVGAPFFSESLRMGVEIFHHLKNVLKSKGYSTNVGDEGGFAPNIKSNEEA
                     IETVLMAIEKAGYRPGEDVLIALDAASTEFYNEKEKIYHFKKSDGRKLDSDEMVDFWK
                     KWVKKYPICSIEDGLAEDDWKGWSKLTDSIGKKVQIVGDDLFVTNTERLKKGIKEEAA
                     NAILVKVNQIGTLTETIEAVQLATRHGFNSVMSHRSGETEDTTIADLAVALNTGQIKT
                     GSASRSDRIAKYNQLLRIEEELLESGIFPGLNLFKRK"
     gene            complement(3896..5032)
                     /gene="carA"
                     /locus_tag="PLG24_06870"
     CDS             complement(3896..5032)
                     /gene="carA"
                     /locus_tag="PLG24_06870"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HMZ24647.1"
                     /translation="MTAYSKRQKAILLLEDGRKFEGYAIGYSGITSGEICFTTGMTGY
                     QEIFTDPSFAGQVVTMTNVHIGNYGVSNKENQSARVQIAGLIVRNFSDYYSRIQADAS
                     LQDYLVENRIVAIAGIDTRALVRHIRSKGAMNCVISTIDTEEKELIQKLSDTPSMAGC
                     ELASIVSTGEPYFYGDEHAALKVAVIDYGCKRNILECFAQRGCYLKVFPARTELSTID
                     AWEPDGYFLSNGPGDPAAMDYAIKSARKILDSNKPVFGICLGHQLLALAQGMKTEKLK
                     YGHRGTNHPVLNTSTGLGEITSQNHGFGVVKDSLIGMETEVEVSHYNLNDGSLEGLRW
                     KNKRIFTVQYHPESSPGPFDSRYLFDEYIAMLKVQRGIPVQVNA"
     gene            5759..>6927
                     /gene="putP"
                     /locus_tag="PLG24_06875"
     CDS             5759..>6927
                     /gene="putP"
                     /locus_tag="PLG24_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HMZ24648.1"
                     /translation="MHIYEAISLGLYMLLMLAIGVYSYRKSTSNSEEFLIGGRRLGAA
                     VTALSAGAADMSGWLLMGLPGAMYFFGVSSAWIAIGLTIGAYLNYVVVAPRLRVYTEV
                     AQNSITLPVFFENRFKDKSHLLKISSSIFILIFFTLYTSAGMVSGGRLFESAFGMDYN
                     TGVWITSLVVVIYTFLGGFLAVSLTDFVQGAIMIIALILVPLVALDKIGGVQETVTLI
                     NSKGVQYLDLFKGTTLVSIISLMAWGLGYCGQPHILVRFMAIDKPAQLAKARRIGITW
                     MIFTVGGALCVGLIGIAYLMKFDTATMEVFDKSKNEAETIFIYFTRVLFHPLIGGFLI
                     SAILAAVMSTISSQLLVTSSSLTEDIYRSLVKKDASPGHFLIAGRLSVLIVAGVAL"
BASE COUNT         1810 a         1658 c         1426 g         2033 t
ORIGIN      
        1 cattggtccc cgggtcgttg aattgagaag cgatcgtacc aaatacaggc atcttgtcag
       61 gggactggtc gaaaagctgg tgatttcgct ggtattgctt tttgacatcg cgtaaagcat
      121 cggctgcccc gcgcttgtca aacttattga tagctacggc atcagcatag tccaacatcc
      181 ctattttctc cagctggcta gccgcaccga attccggagt catgacgtaa agcgaaacat
      241 cagcatattg ggtgatctcc gtatcagact gaccgatacc gcttgattca aggatgatga
      301 gatcaaatcc ggcagccttg agtatattca gggcagcatt aatctgtgcg ctcaaggcct
      361 ggttggattg gcgggtcgcc attgacctca tgtatattcg gggcttattg atcgaattca
      421 tccttatccg gtcgcctaat aaagctccgc ctgtttttct tttgctggga tcgacagaga
      481 tgatgcccat cgtcttatcc ttatattcta gtaagaaacg gcggatcagt tcgtcgataa
      541 ggcttgactt gcctgcacca ccggtacctg ttattcccaa taccggagct gccgatgcct
      601 ctgccttctc attaagcttg ccaagcaatt cccggtgctg atccggataa ttctcagcgg
      661 cagatatgat cctggcgacg acatgtggat tatggctgtt gagttggccg gtattgatat
      721 tcaactggtc tccaaccgga aaatcacaga gacgcaacac atcgtcgatc atgccctgaa
      781 gccccaatgc ccgaccatcg tccggtgaat aaattctcgc aataccataa gcttccagtt
      841 gcttgatttc ttccggcaga atggtacctc ctcctccgcc aaaaaccttg atatgtccgg
      901 catgccgctc tttaagcagg tcatatatat acttaaaaaa ctcgttgtga cctccctgat
      961 aggaagtgat ggctattgcc tgagcatctt cctgaattgc agcattgaca atttcgtccg
     1021 ctgacctgtt gtgacccaga tgtataatct ctgcgccgga agcctgcatg attcgcctca
     1081 tgatattgat ggctgcatcg tgaccatcga agagggagcc tgaagtaacg attctgatat
     1141 gattggttgg acggtaagga gtggtttctt tagccatatg ttaatgcttt acatatgccg
     1201 gtagcactca atttaaggaa ggggctccgg ctctatttcc gcacaaagat agtaaaattg
     1261 cccgtgcacg gcaagtacgc cctcaaaata attggacact ccatttccgt attgtacagg
     1321 attctgtggt tggttttctc atgtagatca ttaccaccga ctcccatctg cacaataata
     1381 atgggagttt aatccggcgc caatgcacat cagatagcct atatttctat tactccctac
     1441 tccccaacac cgggcaataa gctacttgcc gtcgtcctgc ttctttttga atttacctgc
     1501 tttccaggct tcaatgaatt gcttccaggc cattacattc atcagttgga tcggccgggt
     1561 ctggccctga tagtaataag tagaggcgtt ctgacgcata aatgactggg ttgattcttt
     1621 tccgtcaacg ggaactttat catataatga agccaggact cttcggtcga gattctcctg
     1681 aaccctgtct gttatatcgt tattaatgtt cattgccaga aattccgcat ggtagttgtc
     1741 cttgtcaggc catggataaa taactgtttc cggtaaaaga atattgtcaa aagtcatgat
     1801 ttgaaagatg gagtaacgat tggtgctcaa tgtatccggt acgacgtatc ggattgtttt
     1861 gtaacccaca taggtaaaca caatcgtttc ccccttttcc actaccaggg agaagaaacc
     1921 gtcccagttg gtatacgtac cccgactcgt accttcaact gcaatagtca cataaggcat
     1981 cctgacaggc tgtccctttt cctgagtgac cacaacacca ctcaattgga tgggtccgtg
     2041 atacccacgt tgtgcaaaag ctgtaaatcc tgagagggta aaaagaacta ctgagatgac
     2101 aatatactta atattcatgt ccttgttttg atgaaggccg gcttgataca agtccgtaaa
     2161 tttatttcat tataacgtac aaaagtaaaa aatgatgccg actccggcaa aagtggaaaa
     2221 tatttcatgc tttatccttt gacctacttt agtacgtttt aatttataga caaaaatgaa
     2281 ttttcggtca acagcaaata tcttatattt gtaaatattt gaattcctat actttgtatt
     2341 taaatcgaaa tatcttcaac tgaaaaaatc ctaagtttat tgatggatgg aaataaaatt
     2401 aaaaagccgg cggaattctc ctgccggcca ttttttaaca cctttttaat tattccaaaa
     2461 gtcgctgatt tttcgctttg tacctttttc ttgtctgtcg actctaataa aagtaatgaa
     2521 ataatgagcc tgcactctac tttcttttaa agaggttgag acccgggaag attccacttt
     2581 caagcaattc ttcctcgatc ctgagtaatt ggttgtattt ggcgatcctg tcagagcgag
     2641 aagcagagcc ggtcttaatc tggcctgtat tcaaagccac tgcaagatca gctatggttg
     2701 tgtcttctgt ctcacctgac cggtggctca tgactgagtt aaagccatgt ctagtagcca
     2761 actgaaccgc ttcaatggtt tccgtaaggg ttccgatttg atttaccttg acgagaatag
     2821 cgtttgctgc ttcttccttg atgcccttct ttagcctctc tgtattagtg acaaaaaggt
     2881 catcacccac tatctgtact ttcttcccta tactatcagt cagtttggac caacccttcc
     2941 agtcgtcctc tgccaatcca tcctcaatag aacatatcgg gtatttctta acccacttct
     3001 tccaaaagtc aaccatttcg tcagagtcga gctttctgcc atctgatttc ttaaaatggt
     3061 aaattttctc cttttcgttg taaaattcgg ttgatgcggc atccaaagcg atcagtacat
     3121 cctctcccgg tctgtaaccg gctttttcga tagccatcaa cacagtctct atcgcttcct
     3181 cattggattt gatattaggt gcaaaaccac cttcatcacc gacattggta gaatacccct
     3241 tggatttgag cacatttttc aggtgatgaa atatctccac acccatacgc aaagactctg
     3301 agaaaaacgg cgcccccacc ggcataatca tgaattcctg gaagtcgatg ctattatcgg
     3361 catgactgcc tccattcagg atgttcatca ttggaacagg cagggtgcag gcgttgacgc
     3421 caccgatata cctgtacaaa ggctggccgg cttcttttgc agccgctttt gctaccgcca
     3481 ttgaaactcc caaaagtgca ttggcaccaa attttgattt attcttggta ccatctgcct
     3541 ctatcatgac ctgatcaatc tcacgttgca ggaagacatc catccctacc acttcatccc
     3601 tgatttcgtc gtcaacatgc ttgcatgcct tcagtacgcc cttcccgagg aatcgcttat
     3661 tatcattgtc tctcaattcg accgcttcat gttttccggt agatgctcct gaaggcactg
     3721 ccgcacggcc aactgccccg tcttccgtga ggacttcgac ttcgaccgtt ggattccccc
     3781 ggctatccag tatttctctt gatctgatat ctatgattgc gctcatttcc taaaattaat
     3841 agtttttgga gataaatata ttaaattttt ttcaatatca atgtttcttt caattttaag
     3901 cattgacttg cacgggtatt cccctttgca ccttcagcat ggctatatat tcatcgaaaa
     3961 ggtaccgact atcaaaaggt cccggactag actccggatg gtactgcacg gtaaatatcc
     4021 ttttattttt ccacctaaga ccttccagac tgccatcatt gagattgtaa tgggagacct
     4081 ccacttcagt ctccattcca atcaacgaat ccttaactac gccaaatccg tgattttgac
     4141 ttgtgatctc acccagaccg gtagatgtat tcaaaacggg atgattggta ccccggtgtc
     4201 cgtatttcag tttttcggtc ttcatacctt gagcaagtgc aagcaattga tgccccaggc
     4261 aaataccgaa tacgggtttg ttggaatcaa gaattttcct ggcagacttg atggcatagt
     4321 ccatggctgc cgggtcaccc ggcccattgg aaaggaaata accatcaggc tcccatgcat
     4381 cgatcgtact taattctgtc ctggcaggaa acaccttcag ataacatcct ctttgagcaa
     4441 aacactcaag gatattacgc ttgcatccgt aatcaatcac agcaaccttc aatgctgcat
     4501 gctcatcacc ataaaaataa ggttccccgg tagatacaat gctggccaac tcacaaccag
     4561 ccatggaggg tgtatcggaa agcttttgta tgagttcctt ttcttcggtg tcaatggttg
     4621 aaatgacaca attcatggcg cccttactcc tgatatggcg caccaaggca cgtgtatcga
     4681 tgccggcaat agcgacaatt ctattctcta ccagatagtc ctggagggaa gcatctgcct
     4741 gaatcctgct gtagtaatcg gaaaaatttc tgacgatcag tcctgctatc tgcacacgag
     4801 cagactgatt ctccttattg gaaacaccat agttgccgat atgcacattg gtcatagtga
     4861 ccacctgtcc tgcaaaggag ggatctgtga atatctcctg gtaaccggtc atacctgtag
     4921 taaaacatat ttcgcctgaa gtgatgccgg aatacccgat ggcataacct tcaaatttcc
     4981 ttccgtcttc cagcaagagg attgcttttt gacgctttga atatgctgtc attggaaaaa
     5041 tttgcctgca aagatatgga tatattgaca actatacctt tgctaagata aaaaaatagc
     5101 agttattcgg acttaacctt tttggaacat tacctccctt gccgtaacta ctcacgtcaa
     5161 acagcatgta atccaaccaa atatcaacct gattgtggcg tcaaactaat tttcatgccc
     5221 gggtgtttca catagaagag ccgaaatatt tagcttctgc aaatggcaat aaatggcccg
     5281 tgaaatcagc ttggccacca aattgagaaa tctgaatgta ttattcttac taatgattcc
     5341 gacaagcaca aaaaatcact ctgcaccatt ggtacatttt agttagtccg gcaaatcggc
     5401 acttcaaagt caaagaaaat aagccggcat taaatgcaaa ggttgaaaag tgatgaccaa
     5461 ttgtttgcca taaaacaact gataactttg cgatacaaac caacgcaaat ctgacttctg
     5521 ttcccaaatc atcttccaat aataatagtt tcagaaaccg acaaggaaat aaaatacacg
     5581 ccggatgcac acttaccttt gactcatcca atcccttggc attgtgttca aattgacata
     5641 cttttgaatc aaccgataaa aaaaaccgaa aaaactatat actactgcca atttcattta
     5701 aattcgatgc ttgatcggat aataaggaga tcctctccta tccaaaaaga atcatattat
     5761 gcatatttat gaagcaattt ccttaggatt atatatgttg ttgatgctgg cgattggtgt
     5821 gtattcctat cgcaagtcaa cctcaaactc agaagaattt ctcattggag ggcgaaggct
     5881 gggtgcggcg gtaactgcgc tgtcggccgg tgccgctgac atgagtggct ggcttttgat
     5941 gggcctaccc ggtgccatgt actttttcgg cgtctccagt gcctggattg ccatcggact
     6001 tacgataggg gcgtatctca attatgtagt agtggcacca cgactgcgtg tatatacgga
     6061 ggtggcccaa aactccatca cacttcccgt ctttttcgag aatagattca aggacaaaag
     6121 tcacctgctg aagatttcat cgtccatctt catcctgatt ttctttaccc tttatacctc
     6181 ggccggcatg gtatccggag ggaggctctt tgaatccgca tttggtatgg attataatac
     6241 cggtgtatgg atcaccagcc ttgtagtcgt gatttatacc tttttggggg gatttctggc
     6301 tgtgagtctg acagatttcg tccagggtgc catcatgatc atcgccctca tccttgttcc
     6361 tctggtcgct ttggataaga tcggtggcgt acaggaaacg gtgacactga tcaacagcaa
     6421 gggtgttcag tatcttgatc ttttcaaggg tacaaccctc gtaagtataa tttccctgat
     6481 ggcctggggg ctcgggtatt gcggtcagcc acacatcctc gtccgcttta tggccataga
     6541 taagccggct caattggcaa aagcccgacg tataggtatt acctggatga tattcacggt
     6601 tggcggggcc ttgtgcgtag gcctgatcgg tatcgcatac ctgatgaaat tcgacacagc
     6661 tactatggag gttttcgata agtcaaagaa tgaggccgaa accatattta tatatttcac
     6721 acgtgtgttg tttcacccat tgatcggtgg ttttctgatt tcagccatac tggcagcagt
     6781 aatgagtacc atctcctcac aattgctggt cacatccagt tcgctgactg aggatattta
     6841 ccgctctctg gtaaaaaagg atgcaagtcc gggccatttt ctcatcgccg gaagactgtc
     6901 cgtgctgatt gtagccggag tagccct
//
LOCUS       DAOQQN010000152         6889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4573_length_6889_cov_5.641791, whole genome shotgun sequence.
ACCESSION   DAOQQN010000152 DAOQQN010000000
VERSION     DAOQQN010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4573_length_6889_cov_5.641791"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..466)
                     /gene="rpsK"
                     /locus_tag="PLG24_06880"
     CDS             complement(77..466)
                     /gene="rpsK"
                     /locus_tag="PLG24_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HMZ24649.1"
                     /translation="MAKAQKKVKKRKVKVESEGLAYIQASFNNLIISLTNRQGEVISW
                     ASAGKAGFRGSKKNTPYAAQVAATDAGKVAYDAGLRNVEVYVKGPGAGRESAIRAIDG
                     AGIKVTRIFDVTPIPHNGCRPPKKRRV"
     gene            complement(500..877)
                     /gene="rpsM"
                     /locus_tag="PLG24_06885"
     CDS             complement(500..877)
                     /gene="rpsM"
                     /locus_tag="PLG24_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HMZ24650.1"
                     /translation="MARIAGVDLPKNKRGVIALTYIFGIGRSLAGKVLAQAGVSEDTK
                     VIDWTDDNIKTISRIVNDEHKTEGQLRSEVQLNIKRLMDIASYRGLRHRKGLPLRGQR
                     TKNNSRTRKGRRKTVANKKKATK"
     gene            complement(906..1022)
                     /gene="ykgO"
                     /locus_tag="PLG24_06890"
     CDS             complement(906..1022)
                     /gene="ykgO"
                     /locus_tag="PLG24_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HMZ24651.1"
                     /translation="MKVRASIKKRSADCKIVRRKGKLYVINKKNPKFKQRQG"
     gene            complement(1038..1256)
                     /gene="infA"
                     /locus_tag="PLG24_06895"
     CDS             complement(1038..1256)
                     /gene="infA"
                     /locus_tag="PLG24_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HMZ24652.1"
                     /translation="MSKQDIIKQDGIIEEALSNAMFRVRLENDHQILATISGKMRMHY
                     IRILPGDKVSVEMSPYDLSRGRIIYRYK"
     gene            complement(1284..2105)
                     /gene="map"
                     /locus_tag="PLG24_06900"
     CDS             complement(1284..2105)
                     /gene="map"
                     /locus_tag="PLG24_06900"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HMZ24653.1"
                     /translation="MFKPRVKAKSAEEIVKIQESCTIVVKALAHVASIIRPGVSGKFL
                     DKEAETLINDLGGKPAFKGYNGFPASLCISVNDAVVHGIPRDKELQEGDVVSVDCGAV
                     MNGYFGDSAYTFALAPVSDDITKLLRITNESLYKGVEQARVGNRIGDIAFSIQEYTEI
                     KHGYGVVRELVGHGIGKNLHEAPDVPNFGRRGNGLCLEENWTIAIEPMINMGTKHVYQ
                     APDRWTILTKDGKPSAHFEHTVAVKPQGGMILSDHRPIVEEIKNNPNIVNISENI"
     gene            complement(2178..3545)
                     /gene="secY"
                     /locus_tag="PLG24_06905"
     CDS             complement(2178..3545)
                     /gene="secY"
                     /locus_tag="PLG24_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HMZ24654.1"
                     /translation="MKRFINTLKNIWAIKELRNRILYTLLLLLVFRVGSFIVLPGVAS
                     GVLTDVANSRSANDLLGLINIFTGGAFNQASIMALGIMPYITASIIIQVLGFAVPYFQ
                     RLQQKEGESGRKKLNQITRVLTVAITLVQGGAYLTYLKGIGAISPQVSLAIFWGANIL
                     ILTTGTIFAMWLGEKITDKGIGNGVSILIMVGILSRLPQALGFEFTAQMASGGLIMFI
                     LELAAMFVVTMFTILVVQGVRKIPLQFPKKAIGGGSMAAGNRDYIPIKVNGSGVMPII
                     FAQALMFLPAIVAQYLVGTEDTGTATHSIIRALNDNYSFSYNLIYFILIIVFTYIYTA
                     LLINPQQYAEYLKRSNAYIPGVKPGKDTEDYIDAITSKITFPGSIVLGIIAVLPAIAV
                     QFGVNPSFALYFGGTSLLINVAVILDTLTQIESHLLMRKYDGLVASGKLQGRGSGGIQ
                     IGSYY"
     gene            complement(3599..4045)
                     /gene="rplO"
                     /locus_tag="PLG24_06910"
     CDS             complement(3599..4045)
                     /gene="rplO"
                     /locus_tag="PLG24_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HMZ24655.1"
                     /translation="MELNNLRPAKGSNKSGKRIARGQGSGRGGTATRGHKGDKARTGF
                     KNKRAHEGGQMPLQMRLPKFGFKNVNRVDYVGINVSTLEALAEIHNVTEINFDFLTSK
                     KVIKKSEKFKILGAGELSKALTVSAHSASKAAQDKITAAGGTFTTI"
     gene            complement(4074..4256)
                     /gene="rpmD"
                     /locus_tag="PLG24_06915"
     CDS             complement(4074..4256)
                     /gene="rpmD"
                     /locus_tag="PLG24_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HMZ24656.1"
                     /translation="MGKLKITQVKSTIDRNKRQKATIEALGIKKVYRSVVHEDTPAIQ
                     GMIRAVAHLVKVEEVK"
     gene            complement(4264..4770)
                     /gene="rpsE"
                     /locus_tag="PLG24_06920"
     CDS             complement(4264..4770)
                     /gene="rpsE"
                     /locus_tag="PLG24_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HMZ24657.1"
                     /translation="MRPVDAELKDKLVVVNRVAKVTKGGRTFSFAAIVVVGDGQGTVG
                     QGLGKARDVSDAIAKAIDDAKKNLITFPILKGTIPHPQKGKFGAGKVLIRPAADGTGV
                     IAGGSMRAVLEIAGVHNVLAKSQGSSNPHNVVKATIDALSKLRDPHTVAQQRGITVKE
                     LFSGEFEK"
     gene            complement(4814..5164)
                     /gene="rplR"
                     /locus_tag="PLG24_06925"
     CDS             complement(4814..5164)
                     /gene="rplR"
                     /locus_tag="PLG24_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HMZ24658.1"
                     /translation="MKKTKEESRSRIHYRIRKKVAGSAERPRLSVFRSNKAIYAQLVD
                     DTNGNVLTSASSALLETKSDNKVEQAKAVGKLIAEKAKDKNIDSVVFDRSGYLYHGRV
                     KALAEGAREGGLNF"
     gene            complement(5194..5751)
                     /gene="rplF"
                     /locus_tag="PLG24_06930"
     CDS             complement(5194..5751)
                     /gene="rplF"
                     /locus_tag="PLG24_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HMZ24659.1"
                     /translation="MSRIGKAPITIPKGVEFNVDKSNVITVKGPNGTLTRKFDADMIF
                     KTEDGVLTVERPTDSKRHRSVHGLSRALLNGMVEGVSSGFTKKMELVGVGYRANNTGQ
                     LLELALGFSHPIMFQLPDEVKVETKNEKGMPPTIILNSFDKQLIGQVAAKIRSFKKPE
                     PYKGKGVRFAGEVLRRKAGKTAGKK"
     gene            complement(5781..6185)
                     /gene="rpsH"
                     /locus_tag="PLG24_06935"
     CDS             complement(5781..6185)
                     /gene="rpsH"
                     /locus_tag="PLG24_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018679504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HMZ24660.1"
                     /translation="MALNDPIADYLTRIRNAQQAGHRIVEIPSSKMKKKITEILYENG
                     YILKYTFDDAAGKQGIIKIALKYNEATKKPVIQELKRVSRPGLRQYRHCEELPRVING
                     LGIAIISTSHGLLTDKQARAQNVGGEVVCFIS"
     gene            complement(6230..6499)
                     /gene="rpsN"
                     /locus_tag="PLG24_06940"
     CDS             complement(6230..6499)
                     /gene="rpsN"
                     /locus_tag="PLG24_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HMZ24661.1"
                     /translation="MARKSVIARERKRERMVAKYAEKRKQLKEAGDYDALDKMPRNSN
                     SIRLHNRCKLTGRPKGYMRDFGICRVMFRKMALDGKIPGITKSSW"
     gene            complement(6525..>6889)
                     /gene="rplE"
                     /locus_tag="PLG24_06945"
     CDS             complement(6525..>6889)
                     /gene="rplE"
                     /locus_tag="PLG24_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HMZ24662.1"
                     /translation="ISGQKAVVTKARKSVSNFKLREGMPIGVRVTLRNNRMYEFLDRL
                     ISISLPRVRDFKGVNDKSFDGRGNYTMGITEQIIFPEIDIDKIAKVLGMDITFVTTAK
                     TDKEAHALLSALGIPFKK"
BASE COUNT         1814 a         1621 c         1262 g         2192 t
ORIGIN      
        1 aaaagctctt gccttcttgg ttgcaggacc tgtatatctt gccataatat agttctactt
       61 tataaaataa tagtgattaa actcttcttt tcttaggagg gcggcatcca ttgtgtggga
      121 tgggggttac gtcaaaaatc ctggtaacct taatacccgc tccatcgatg gctctgatag
      181 cggattctct tcccgctcca ggtcccttga catacacctc aacgtttcta agccctgcat
      241 cataagccac tttgcctgca tcagtagctg cgacttgtgc agcgtaagga gtgttctttt
      301 tagaccctct gaacccggcc ttaccggcag atgcccagga aatcacttct ccttgtctgt
      361 tggtcaaaga aataatcagg ttattaaagc ttgcctgaat ataagccagt ccttcagact
      421 cgactttaac ttttcttttc tttactttct tttgtgcttt agccataatg ataagctata
      481 aaaacaatgt tatttattat tatttggttg ccttcttctt gttagcaaca gttttacgtc
      541 ttcctttacg tgttctggag ttattcttag tcctctggcc tctcaatggc aagcctttac
      601 gatggcgcaa accacggtaa ctggcaatat ccatcaatcg cttgatatta agctgcactt
      661 cagacctcaa ctggccttca gtcttgtgtt catcattgac aattctggag atggtcttga
      721 tattatcatc tgtccagtca ataactttag tatcttcact cacacctgcc tgcgcaagca
      781 ccttaccagc cagtgaacgg cctatgccaa aaatataagt aagggcgatg acaccccttt
      841 tgttttttgg taaatcaact cctgctatac gagccatata attactatat tataattgct
      901 aaatattatc cttgtctttg tttaaactta gggttctttt tgttgattac atacaactta
      961 ccctttcttc tgacaatctt gcaatccgca gatctttttt taattgatgc tctaactttc
     1021 atcagtcaaa attttaatta tttatatcta taaatgattc ttccacggga caaatcataa
     1081 ggagacatct ccacagaaac cttatcaccg ggcagaatac gtatatagtg cattctcatt
     1141 tttcccgaaa tggttgccaa aatctgatga tcattctcaa ggcgtaccct gaacatggcg
     1201 tttgacaacg cctcctcaat aataccgtcc tgcttgatta tatcctgttt tgacatacgt
     1261 cctttttttc ggagtgcaaa tatctaaata ttttctgaaa tattcacaat atttgggtta
     1321 ttttttattt cttccacaat cggtctgtga tctgacagta tcataccacc ttgaggcttc
     1381 actgctactg tatgttcgaa gtgggctgaa ggcttgccat ccttagtgag tatggtccag
     1441 cgatccggag cctgatagac atgctttgta cccatattga tcatcggttc gattgcaatg
     1501 gtccagtttt cttccaggca caatccgttc ccccttcgtc caaagttcgg cacatcaggt
     1561 gcttcgtgga gattttttcc aataccatgg cccaccaact cccttacgac accataaccg
     1621 tgctttatct cggtatattc ctgaatcgaa aaggctatat caccgatacg atttcctact
     1681 cttgcctgct ctacaccctt atacagggat tcattcgtga tacgcagtaa tttagtaatg
     1741 tcgtcgctga caggtgccag ggcaaaagta tatgcggagt ctccaaaata tccattcatc
     1801 actgccccgc aatcaaccga caccacatca ccttcctgca actccttatc cctgggtatt
     1861 ccatgtacta cggcatcatt cactgaaata cacaaagaag caggaaaccc attataccct
     1921 ttgaatgcag gcttgccgcc caggtcattg atgagtgttt ctgcttcttt gtccaaaaac
     1981 ttcccggaca ctccgggcct tatgatcgat gctacatggg caagggcctt aacaactatc
     2041 gtgcagcttt cttgtatctt gacaatctct tcggcactct ttgctttaac tctgggttta
     2101 aacatgagtc cgaagttaaa atgtattaat tgatttcact tcagaaaatc cgatcattcc
     2161 ggtatcccgg tgtttcatca gtaataggat ccaatttgta ttccgccgga tccccttccc
     2221 tgaagtttac cggaagcaac caaaccatca tactttctca tcaagagatg actttctatc
     2281 tgtgttaatg tgtccaaaat tacagcaaca ttaatcaaaa gtgatgtacc accgaaatac
     2341 aatgcaaatg atggattgac accaaattgt accgcaattg ccggaagcac agctatgatg
     2401 cccaatacaa tagatcccgg gaaagtaatc ttggatgtaa tagcatcgat ataatcttct
     2461 gtgtccttgc ccggttttac accaggtata taggcattgg atcttttcag gtattcggcg
     2521 tattgctgag gattaatcaa caaagcagta tatatatagg taaatactat gattagtata
     2581 aaatatatca ggttgtaaga gaatgaatag ttatcattca atgcccgaat aatgctgtgg
     2641 gtggccgtgc cggtatcttc tgtaccaacc aggtactggg cgacgatagc aggcaaaaac
     2701 atcagtgcct gtgcaaagat aattggcatt acacctgagc cattcacttt aatcggaata
     2761 taatccctgt ttccggctgc catacttccg ccaccgatcg ctttctttgg gaactgaagc
     2821 ggaattttcc tgactccttg aacgaccagg atggtaaaca tagtaaccac aaacatagct
     2881 gccaattcaa gaataaacat aatcaatcca cctgatgcca tctgagctgt aaattcaaag
     2941 cccaatgcct gaggcaatct ggaaaggata ccgaccataa ttaagatgga aacaccgttt
     3001 ccgatacctt tatcggtaat cttttcgcca agccacatgg caaagatggt acctgtagtg
     3061 aggattaaga tattggcacc ccagaaaatt gccagagaaa cctgaggcga gatagctcct
     3121 atgcctttta gataagtaag gtaggcacca ccctgcacca atgtaatagc aacggtcaat
     3181 actctggtaa tttgattcaa cttctttcta ccggattcac cttccttctg ctgcaaacgc
     3241 tggaaatagg gaacggcaaa acccaaaacc tgaataataa tcgaggctgt gatataaggc
     3301 atgataccga gggccatgat tgaagcctgg ttgaaggctc caccggtaaa gatattaatt
     3361 aatcctaaca ggtcattagc gcttctgcta ttcgcgacat cggtcaacac tcctgatgcg
     3421 acacccggca atactataaa agaccccact ctaaagacca aaagcagtag aagcgtataa
     3481 agaatacgat ttcttagctc cttgatggcc caaatatttt ttaaagtgtt gataaatctt
     3541 ttcatcctta atcttaatat ttaaatgatc tccctaaggt tattcagggc ttcgtatatt
     3601 aaatggttgt gaatgttcct ccggcagcgg taattttatc ctgtgcagct ttggaggcag
     3661 aatgtgctga tacggttagt gctttggaca actcccctgc acccagaatc ttgaacttct
     3721 cagacttctt tattaccttc ttagaggtca gaaaatcaaa gttgatctca gttacattgt
     3781 gtatttcagc caaagcctcc agtgtgctta cgttgatacc gacataatcc acccgattga
     3841 cattcttgaa accgaactta ggaagtcgca tctgaagagg catctgtcca ccttcatgag
     3901 cgcgcttgtt tttgaaacct gttcgtgcct tatctccctt atgaccacga gtggcagtac
     3961 cgcctctacc gcttccctgc cctctggcaa ttcttttacc tgacttgttc gaaccttttg
     4021 ccggtcttag attatttaat tccatcggaa taatttactt aattatgtaa atattatttt
     4081 acttcttcca ctttcaccag gtgagctacc gctctgatca taccctggat ggcaggtgta
     4141 tcttcgtgga ctaccgaacg gtaaaccttc ttgataccta atgcttcaat ggttgctttt
     4201 tgtcttttat tacggtcaat tgtacttttg acctgagtta ttttcaactt tcccatgatt
     4261 caattatttt tcaaattcac cactgaacaa ttccttgacg gtgattcctc tttgctgagc
     4321 aaccgtatga ggatctctta atttggataa tgcatcaatc gttgccttta caacgttatg
     4381 aggattggag ctaccctgtg acttggccaa tacgttgtgt actcctgcga tctccagtac
     4441 agctctcatg gaacctccgg cgatcacacc ggtaccatct gctgcaggtc tgatcaacac
     4501 ctttcctgct ccaaatttcc ccttttgagg atggggtatg gtacctttca gtattgggaa
     4561 ggtaatcaga tttttcttgg catcgtcaat cgccttggca attgcatcag atacatccct
     4621 tgccttaccc agtccttgtc cgaccgtacc ttgtccatca cctaccacta cgatggcagc
     4681 aaagctaaat gtacgtccac cctttgttac ttttgcaacc cggttgacaa ctacaagttt
     4741 gtccttcaat tcggcgtcaa ctggacgcat tctattttgt ggttgttttg ccattattct
     4801 ttagctaata aatttaaaaa ttcaaaccgc cttcgcgagc tccttctgct aatgctttca
     4861 ctctaccgtg atacaaataa ccgctacggt caaaaaccac cgagtcgata ttcttatcct
     4921 tggctttttc tgcaatcaat ttgccaacag ccttagcctg ttcgacctta ttatctgatt
     4981 tagtttccaa cagagcagaa gacgctgaag tcaatacatt accattggtg tcatcaacca
     5041 actgagcata gatagctttg ttgctacgaa acacgcttaa tcgtggtcgt tcagctgaac
     5101 ctgcaacttt ctttctgatt ctgtaatgaa tccgcgatct ggattcttct tttgtctttt
     5161 tcatatctaa tgatatagag aattaaacgg attttatttc ttacctgcag tttttccagc
     5221 ttttctgcgt aaaacttctc cggcaaatcg aacacccttt cctttatagg gttcaggttt
     5281 cttaaatgag cgaatcttgg ctgcaacctg accgatcaat tgcttgtcaa agctattaag
     5341 tatgatagta ggtggcatac ccttctcatt ttttgtctcc actttcactt catcaggaag
     5401 ttggaacatg ataggatgtg agaaaccgag tgcaagctca agtagttgtc cggtattatt
     5461 cgctctgtaa ccgacaccta caagttccat tttcttggta aaaccgctag atacgccttc
     5521 caccatacca ttgagaaggg cacggctcag tccgtgaaca gagcggtgac gcttggagtc
     5581 ggtaggtctt tcaacagtca atactccatc ttcagtctta aagatcatat cagcgtcaaa
     5641 cttcctggta agggtaccat taggaccttt aacagtaatt acatttgact tatcaacatt
     5701 aaactccact cctttcggga tcgtaatggg agctttacca attcttgaca taattaatta
     5761 tttctttctt tctcaaaaaa ttagctaata aagcaaacaa cttcaccacc gacattctgt
     5821 gcccttgcct gcttatcagt cagcagaccg tgtgatgtag agatgatagc gatacccaga
     5881 ccattgataa ctcgtggtaa ttcttcgcag tgacgatatt gtcgcaatcc cggtcgactt
     5941 accctcttga gctcctgaat aacaggcttt ttagttgcct cattatattt aagtgcaatc
     6001 tttataatac cttgcttacc ggcagcatca tcaaaggtat acttcaggat atagccattt
     6061 tcatacagga tttcagtgat tttcttcttc attttggaag agggaatctc cacaattctg
     6121 tgtccggctt gttgagcgtt tctgattctg gtcaggtaat ctgctattgg atcattcaga
     6181 gccattctat tataatttag tttgctataa ttaatttatg ggaatattgt taccaggagg
     6241 atttcgttat accaggaatc ttgccatcca gagccatttt tctaaacatt accctgcaga
     6301 taccgaagtc acgcatataa ccttttggtc gaccggtcaa tttgcaacgg ttgtgcaacc
     6361 tgatcgaatt agaattgcgt ggcatcttgt ccaaagcatc ataatctcct gcttctttca
     6421 gttgttttct tttctcggcg tatttagcaa ccatgcgttc tctttttcgc tcgcgtgcta
     6481 taactgattt tcttgccatc ctgaaatatt atttgtcttt attattattt tttaaatgga
     6541 attcccaatg ctgacaacaa agcgtgagct tccttatccg tctttgctgt agtaacgaat
     6601 gtgatatcca taccaagtac tttggcgatc ttatctatgt ctatctccgg aaagataatc
     6661 tgttcagtga tgcccatagt atagttacca cggccatcga aactcttgtc attgactcct
     6721 ttgaagtccc taacccgagg cagggatatt gaaataagac gatccaggaa ttcgtacatt
     6781 ctattgttgc gcaatgtcac gcgtacaccg atcggcatac cttctctcaa cttaaagttg
     6841 gagacggatt tgcgggcttt agtaacaacc gccttttgtc ctgaaatgg
//
LOCUS       DAOQQN010000153         6887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4575_length_6887_cov_5.616657, whole genome shotgun sequence.
ACCESSION   DAOQQN010000153 DAOQQN010000000
VERSION     DAOQQN010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4575_length_6887_cov_5.616657"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLG24_06950"
     CDS             <1..275
                     /locus_tag="PLG24_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24663.1"
                     /translation="FLLYVERMKRINFYAHLIQFGDFTSPDVAAIRHKYRIPFRFWDI
                     LTTYCHRTYALHVPDKSSLLKYPKFVVAFFSHLGRLIQTIRFLKST"
     gene            complement(613..2121)
                     /locus_tag="PLG24_06955"
     CDS             complement(613..2121)
                     /locus_tag="PLG24_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24664.1"
                     /translation="MPKINNLEMTNLKYRRQFLFSPIECKKLEGWQTEKVDNYFIYVH
                     PDCKFEKSSGVNDLYLIGYFCDPHHETRSSQDILNNLSQLNNINDFPNKLYSLVGRFV
                     LIIKTENDFIFFNDPCGFKTIYYTKDNDKFFAASQPLLIKEVVDLKQTRFYDDYFNSN
                     YVAENIENWVPSGFTFYKNTYHLIPNHYIKISEPIQVRYYPFKEIELINYSELVIKFS
                     NLLKKIVLAAHRNMDLTFTLTAGWDTRIILSCCKDYIDGMTFYTLKYRGMNAAHPDIK
                     IPMLLSRTLKLNFDIYNCQKEITPEFSDVYKSNTDMAHIDDWGKIAFGMSKSYPDKKV
                     AVKGVCSEVGRCFYYPNGKVNYTITINDLLNLESGWVELGFLQEEIRKWYLSLEKNCY
                     NYNLYDLFYWEHRMGSWQAQSQLEWDIVQEVFTPFNSREIIDLMFSIEASKREKYHPQ
                     LYLDAIKYLWKETLRQPINPIDNKTKIKNTVIKILKKIGLLQGLKSIMKKIS"
     gene            2451..4532
                     /locus_tag="PLG24_06960"
     CDS             2451..4532
                     /locus_tag="PLG24_06960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ24665.1"
                     /translation="MKYTVTLCLLLTFLCFTPASGQWTPTVGPFGSTHVATLLAKDSK
                     LVASTTCGTFIKDAASESWVRYPANRFISYTVKDGDIYACSYFDPHGIWILDPNHVDE
                     IPQSIFPGSTLLIASSDDFLFKYADYSGVSRSADGINWTDYNDGLPQENSGGFAEYDI
                     SAMTVARPFIYCSTDKGIYRNDINPTTWTLANNGLPEEKATFINQFDNTLYTAIGKTL
                     YKSSDQGANWSEFYTAAGQVTAMLKSGGDIYLGTENGVLMSTDGGSQWQSLSDGLKDN
                     HITCLTMYQSEVVCGTDLDGIYILKGSEWQRYGIGLNCEPAFTLTGNADGQSLITASY
                     GRMFSYDNQANTWTALKVPYEGDQTITLTKLGLPVMDQDTIFAFAELLANNSGPKFSL
                     IYSTDNGNTWQGDNNAQPFGYTIPEILKINNRIFGSSGDVFATTNNGGITWKNITPED
                     PLSLDAHNWNGYNGYIYGITGANRIIRFDNNYKIPKWKEVDSDIPADKKILAIANIDE
                     ALFAMAPGEIYVSTDDAASWSRVDVTLPFERIRNWTYYKNILFVTCDQGIFYTKDRGN
                     TWTSLNDGLESPDLTVVKIAFDHIFVVNNAVTVLKRSISELLSTSENLAENQMMLYPN
                     PADLYFTIPFIPGESNLQYRISDMKGAVVRSGKTADGGQVNVNNLTAGMYIVTVMSEK
                     GNLTGKLIIQR"
     gene            complement(4908..6380)
                     /locus_tag="PLG24_06965"
     CDS             complement(4908..6380)
                     /locus_tag="PLG24_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24666.1"
                     /translation="MKAINSNRFYTRIFIFIMFAQGVAAQDYSPVRENLPPYIFETSK
                     EYDESASMFMRGTMDSVINNGPVRDFYPYNTLFFGDNGNLSCLYMLHHSWLGARISVD
                     DSEDAATFLYQNVSNNSFPLITYKLRLEYSGNPGSQWMADNDFSILATVVSVEQTSWK
                     GIIDDSIKIIRLQYSGSNAQIKWSSGHIVISKHHGILSFPDLRYFPFEWNTIELAGGP
                     GFPVFDPSARDMYYMKAGQSYIAASKYGFGVKYPKYKEVRCLEILSDVQNTVTRRVQI
                     INLTYSYTDQKWKSDTSVKVEVIDFDSIIINQPVDRFFKLKGVDNIYGHIIMNKHRQK
                     YYYLSAAPGANNCVPVYTDQCAQYGFDGGFDTYWDCPMFVEFDYNQPVFIDYGDHTWG
                     HDINIDSLLTTRKISKLEGVTISPNPASSHFYMTIPPTVNLSKVFMTDILGNRVAIKV
                     EKMTAEKMELNTDVPGGVYLITGVDEAGNTYFLGKVVIYR"
     gene            complement(6591..>6887)
                     /locus_tag="PLG24_06970"
     CDS             complement(6591..>6887)
                     /locus_tag="PLG24_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24667.1"
                     /translation="DYQYYITSKIFSESDFHHSMNIETEAINALYAGLSAPYALKYPK
                     TSALNDFYIKKAYVALSLGLKEIANDLITQRIKKGLSIKTFLRWSKLKWEYLTQ"
BASE COUNT         2088 a         1336 c         1320 g         2143 t
ORIGIN      
        1 gatttctgct ttatgtggaa agaatgaaaa gaatcaattt ctatgcccac ctcatccagt
       61 ttggcgactt cacaagtccg gacgtggcgg ctatccggca taagtatcgt attccattcc
      121 gattttggga tatattgacc acctattgtc accggactta tgcccttcac gtgcccgata
      181 aatcaagtct tctgaagtat cctaaatttg ttgtggcctt tttcagccac cttggtcgtc
      241 tcattcagac cattcgcttt ttgaaatcaa cctaagcgat ttttttcaaa tattggaaga
      301 ataggatatc tcatgcaatt cattctgcta tcctattgaa gtgctaatat caattgttac
      361 aattgaatac tatcagccag ttgtcggaag tgagttctgt gggggagtgg ccttgttgaa
      421 ttaagcgaat gagacagtga attgccaaaa attagcgaag ggatatacag tagagcatca
      481 gcggagtgca acaatccggc ttcggggatg cagattttac agtcattaag gtcaatgaaa
      541 ttgaactatt acgaatgttc attcggtatt acttttttat caagaaaaaa gtaattaata
      601 acataaaaag tactatgata tttttttcat aatactcttt aatccttgaa ggaggcctat
      661 cttttttagt attttaatta ctgtattttt aatcttcgtt ttattatcaa ttggattgat
      721 tggttgcctt aaagtttctt tccataaata ttttattgca tccaaatata attgaggatg
      781 gtacttttcc cttttagatg cttcaatact aaacattaaa tctattattt ctcttgaatt
      841 gaaaggtgta aaaacttctt gaactatatc ccactctaat tgactttgag cttgccaact
      901 acccatacga tgctcccaat aaaaaagatc atataggtta taattgtaac aatttttttc
      961 taaagataaa taccattttc ttatttcttc ttgtaaaaag cctaattcta cccacccaga
     1021 ctccaaattt aataaatcat ttatggttat agtgtaatta acttttccat tagggtaata
     1081 aaaacaacgg ccaacttctg agcaaacacc ttttacagct acttttttgt caggatagct
     1141 tttagacatt ccaaaagcaa tttttcccca atcatcaata tgtgccatat cggtattaga
     1201 cttatataca tcactaaatt ccggagtaat ttctttttga caattataaa tgtcaaaatt
     1261 taatttcaac gtcctagaca ataacattgg aattttaata tcaggatggg cagcattcat
     1321 tcccctatat ttcaaagtgt aaaatgtcat accgtcgata taatctttac aacaacttaa
     1381 aataattctg gtatcccatc ctgctgtcaa agtgaatgtt aaatccatat tgcgatgagc
     1441 cgctaaaaca atttttttca atagattgga aaattttatt actaattccg aataattaat
     1501 caattctatt tctttaaaag gataatacct tacttgaata ggctctgata ttttgatata
     1561 atgatttggg attaagtgat atgtattttt ataaaatgtg aatccacttg gcacccaatt
     1621 ttctatattt tcagcgacat agtttgaatt gaaataatca tcataaaatc ttgtttgttt
     1681 taaatcgaca acttccttaa ttaatagagg ttgcgatgca gcaaaaaatt tatcattatc
     1741 tttggtatag tagatagtct taaatccaca agggtcgttg aaaaaaataa aatcattctc
     1801 tgttttaata attaatacaa atctcccaac caagctgtat aatttgtttg gaaaatcatt
     1861 gatattatta agttgagaaa gattattaag aatatcttgt gatgatctgg tttcatggtg
     1921 ggggtcacaa aaataaccaa tcaagtacaa atcatttacg ccacttgact tttcaaactt
     1981 gcaatcagga tgtacgtaaa taaaataatt atctactttt tcagtctgcc aaccttccaa
     2041 tttcttacat tcaatcgggg aaaagagaaa ttgacgtcta tactttaaat tggtcatttc
     2101 caaattatta attttaggca atatggcttt ttttaatgaa aaacttaaaa aaatattctg
     2161 aatatgagtt aatgaaagac cagaacggta taatagaaaa aacaaatcat ggactattaa
     2221 attttgtcac actttattgg atagtctctt agttatcgaa ttggtcaaag tcaattcgat
     2281 ctgattttgc ttttaggcta cagcatatgt tcatcataat agtagaaaag tgttggtaat
     2341 tatacacaat ctattgttac tcaaaatatt ttttcatatt tttgttctga taccgtaaat
     2401 tcaaaagtta catttcaggt tatcttttat ttgctttaaa aatcatttca atgaaataca
     2461 ccgttacact ttgtctgtta ttgaccttcc tctgctttac acctgcctcc ggccagtgga
     2521 cgcctaccgt cggacctttc ggatctacac acgtggcgac ccttcttgcc aaggactcca
     2581 aactggtggc atcgaccact tgcggtacct ttatcaagga tgcggcatct gaaagttggg
     2641 ttcggtatcc ggccaatcga tttatctcct atacggtaaa ggacggtgat atatatgcct
     2701 gttcttattt tgatccacat ggtatctgga tcctggatcc caaccacgtc gatgaaattc
     2761 ctcagagtat ttttccgggt tctacattgc tgattgcttc atcagacgac tttttgttca
     2821 agtatgccga ctacagcggt gtatcacgtt ctgcagacgg catcaactgg acagactata
     2881 atgacggact gccgcaggaa aattccggtg gatttgctga atacgacatc agcgccatga
     2941 cggttgcccg gccatttatt tattgcagta cagacaaagg aatttatcgc aatgacataa
     3001 atccaactac atggaccctt gccaataatg gtctgccgga agagaaagct acctttatca
     3061 atcagtttga caatacactt tatactgcca tcggcaagac gctctataaa agtagtgatc
     3121 agggagcaaa ctggagtgag ttttatacgg ctgccggtca ggttactgcc atgttaaagt
     3181 cgggaggcga tatatacctt ggtacggaga acggtgtgct gatgtcaaca gatggcggat
     3241 cacaatggca atccttgtct gatgggctga aagacaatca catcacctgc ctgaccatgt
     3301 atcagtccga agtggtctgt ggtacggatc tggatggcat atacatcctc aaaggaagtg
     3361 aatggcagcg atatgggatt ggtctcaact gtgaaccggc ttttacgctg acaggaaatg
     3421 cggatggaca aagcctgatt acggcttcct atggtagaat gttcagctat gacaatcagg
     3481 ccaatacctg gacagccctg aaagtgccgt atgaaggtga tcagacgatt actttgacta
     3541 aactgggcct tccggtaatg gatcaggata ccatctttgc ctttgctgaa ttgctggcta
     3601 ataatagtgg accaaaattc tcactgatat actccaccga caatggaaat acatggcaag
     3661 gcgacaacaa tgcgcaacct tttggttata ccatacctga aatcctgaaa ataaataata
     3721 gaatcttcgg ttcaagtggt gatgtatttg caaccacaaa caatggcggc ataacatgga
     3781 aaaacattac acctgaggat ccgctcagct tggatgccca taactggaac ggctacaacg
     3841 gctacattta cggcatcacc ggagcgaaca gaattatccg gtttgacaat aattataaaa
     3901 ttcccaaatg gaaggaggtg gactccgaca tcccggctga taagaaaatt cttgctatag
     3961 ccaacatcga tgaagctttg tttgcaatgg ctccgggcga aatctatgtc agcacggatg
     4021 acgctgccag ctggtctcgt gtagatgtaa cacttccgtt tgaaaggatc agaaactgga
     4081 cgtattataa aaatatacta tttgttacct gcgatcaggg cattttctat acaaaggacc
     4141 gtggcaatac ctggacctct ctcaatgatg gtctggaatc acccgatctg actgttgtca
     4201 aaatagcctt tgatcatatt tttgtcgtca acaatgcagt taccgtgctg aaaagatcga
     4261 tttctgagtt gctttcaacc tccgaaaatt tagcagaaaa tcaaatgatg ctttatccca
     4321 acccggctga tctatacttt actattccat ttattcccgg agaaagcaat ttacaatacc
     4381 ggatcagtga catgaaggga gctgtcgtgc gctccggcaa aaccgctgat ggtggtcagg
     4441 taaatgtgaa taaccttact gcaggtatgt atatcgtgac ggttatgtca gaaaagggta
     4501 atttgacagg aaagttgatt attcagagat agcttctcat gtagcgtcaa gtcgattctc
     4561 ccggatcatt tgtcagaatg gtggtcgaat acaatattcg ggtattttca gacaaccata
     4621 actatgaagt ttctatcgat taaccggaat ttgtgtacag gatgaaaaac attttgatct
     4681 ttcgatatgg gagtcgatca taatacaact tggtttttag gcgataaaac atcatccgat
     4741 aacggctaag tgctgcctaa tcacgataat ccaagcaaat cgggcaaaag agacgaaaaa
     4801 aaacaagggt aaattttacc tctattgtca gaacgagtgt aaatatttcg aatttttacc
     4861 ctcgtattag agatgaatgt agcatttaca tttgaatgaa ataaccttta tctatatata
     4921 actactttcc ctaagaaata tgtatttccg gcttcatcca cgcctgtaat caggtaaaca
     4981 ccgcctggaa catccgtatt cagctccatt ttctcagcag tcatcttttc gacttttatg
     5041 gcaactctgt ttccaaggat gtcagtcata aaaactttac tcaggttgac cgttggaggg
     5101 atggtcatgt aaaaatgact ggaggccgga ttggggctga tggtcacacc ttccagttta
     5161 cttatctttc gggtagtcag caggctgtcg atgttgatgt catgacccca ggtatggtcg
     5221 ccataatcga taaacaccgg ctgattataa tcaaactcga caaacatcgg acaatcccaa
     5281 taagtgtcaa aaccgccgtc aaatccatat tgagcacatt ggtcggtata aacaggcaca
     5341 caattatttg ctccgggtgc agcgctaaga taataatatt tttgtcgatg cttattcatt
     5401 ataatatgcc cataaatatt atcaacgccc tttaatttga aaaaccggtc aaccggctga
     5461 ttgatgatga tggagtcaaa gtcaataact tcgaccttaa ccgaggtgtc gctcttccat
     5521 ttttgatcag tataagaata tgttaaatta attatctgaa cccttcgtgt aactgtattt
     5581 tgcacatctg agagtatttc caggcacctg acctccttgt atttaggata cttaacacca
     5641 aagccgtatt tggatgcggc aatataactc tgcccagcct tcatatagta catgtcccgc
     5701 gcactcgggt caaagacagg aaagccggga ccgcccgcca gctcgatggt gttccactca
     5761 aaaggaaaat aacgtaggtc agggaaggaa agaatgccgt gatgcttgct tatgacgata
     5821 tgtcccgatg accatttaat ctgtgcattc gacccggagt attgcagacg aatgatcttg
     5881 atactgtcgt caatgattcc cttccaggat gtctgttcta ctgaaacgac ggtagccagg
     5941 atgctaaaat cattgtctgc catccactga gagcccggat tgccggaata ttcgaggcgg
     6001 agtttatagg taatcaaggg aaagctatta ttggaaacat tctgatacag gaaggtcgct
     6061 gcgtcctcac tatcatctac cgatatacgg gcccccagcc agctgtgatg cagcatgtat
     6121 aaacaactta agttgccatt atccccaaaa aacaaggtgt tgtaaggata aaaatcccgg
     6181 accggaccat tatttatgac ggagtccatg gttcctctca taaacatact tgcactttca
     6241 tcgtattctt ttgatgtttc gaagatgtaa ggaggtaggt tttctctcac aggtgaataa
     6301 tcctgagctg ccactccttg tgcaaacatg atgaaaatga atatccgggt gtaaaatcga
     6361 ttggaattaa ttgctttcat aagccagtga tttttacaga aaatgatgga cccgtgtaaa
     6421 tttaataaaa tattttaata atgacctggt tttatcctat gagatgctga attccagagg
     6481 tttcgacaaa ggaatcgacc cgaattgaca agattgccac cacaattttt taaaacaatg
     6541 atatggaaga agtcagatcg aatagggctt tttggggatt tattattttt ttactgtgtc
     6601 agatattccc acttcaactt gctccacctc aaaaaggtct ttattgatag tcctttttta
     6661 atccgttgag taatcagatc gttggctatt tccttaagtc ccagagacag ggcgacatag
     6721 gcttttttga tataaaaatc attcagggct gaagttttcg gatactttaa ggcgtatggt
     6781 gctgaaaggc cggcatataa ggcgttgata gcctctgttt cgatgttcat ggagtgatgg
     6841 aagtctgatt ccgaaaatat tttggatgtt atataatatt ggtaatc
//
LOCUS       DAOQQN010000154         6885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4577_length_6885_cov_5.977599, whole genome shotgun sequence.
ACCESSION   DAOQQN010000154 DAOQQN010000000
VERSION     DAOQQN010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6885
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4577_length_6885_cov_5.977599"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PLG24_06975"
     CDS             <1..366
                     /locus_tag="PLG24_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24668.1"
                     /translation="KVKPQKKDLLVAKHQELLSFTDLAKAVNRLLSKSGFFYIILPGK
                     EAVEFEKIASSFSIHPVGKLFISSFKGSGVIREITVYQKAVISEIVNSEHLFLYRSGM
                     RNDWSEEYRQMLRKFKKFD"
     gene            complement(370..621)
                     /locus_tag="PLG24_06980"
     CDS             complement(370..621)
                     /locus_tag="PLG24_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HMZ24669.1"
                     /translation="MNITFDELRRIKHSLPTGSINKIAKELNIEEQTVRNYFGGDHYD
                     SGGEVVGLHIEPGPEGGIVYLEDTRIIEAARRILESSSN"
     gene            complement(844..2886)
                     /locus_tag="PLG24_06985"
     CDS             complement(844..2886)
                     /locus_tag="PLG24_06985"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HMZ24670.1"
                     /translation="MTRFINSFLFTAFMATLFLGCVKTDIDEPPYNTNYPTYNANFSI
                     KDLRALHTNGKFETINEDYLIKAVVVADDKSGNYYKTLVVQDSTGGMEVKINAVGLFN
                     TFPVGRRVYLKLKGLTLGDYNGNFQIGGGTYLDDSNNNRLGGIEEPVVNQYILKGEFN
                     VPITPAVKTIAELGDGDINTLVQLNGLEVPEADLGATYADAVNKTTTNRTLKDCSNKT
                     ITLRTSGYATFAGLEMPQGNGTIVAIYTVFGATKQLFLREASDADFSGKRCGDGGVTG
                     ELINISDVRALYQGSTISLPDNKKIKGIVISDKTYANITNKNLVLWQSGNAGITLRFT
                     ETVPFNVGDELEIGISGLELSEFNGLLQINNAPLTNVSRTGTGKTVTPTELSIDNLNA
                     NIKVYESQLVKIKGVTLSKSNGTTFSGTVNAKDATGSIALYTTSYSTFANDNFPTGTV
                     DIVAIASRGGSNSENQLTIRSKDDISGGVVNPGETIDISAVRALYAGAATTVPASKSI
                     KGIVISDKTFSNITSKNVVIQQEGNAGIVVRFSANNTLNLGDEVEIAIGGMELSEFNG
                     LLQLNNVPNANAKVVGTGKSINPVTITLNELVSNLENYESTLIKVVGVTLSKSSGSTF
                     EGTVVMTDATGTANMYTTSYATFAKDNFPTGVVNVTGIVGQFDTPQVNIRSKADIN"
     gene            complement(2917..5709)
                     /locus_tag="PLG24_06990"
     CDS             complement(2917..5709)
                     /locus_tag="PLG24_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ24671.1"
                     /translation="MLRFNLVVFFILTFLFSSFKAYNQSVFVFQGILKEADNSNPVED
                     AMVMLHPLGIGTTTDKVGFFKIEYTGNFSGNIHIKKEGFIDVDYAIQPADVNAAVPVI
                     ITMYRDAAYNARVQSTGTIPVISIDDESANDGSQEISSLLSASRDPFLSIAGYNLGVF
                     RFRLRGYDTEQNSVYLNGIQMNDPENGALVFGEWGGLNDVLRNTDANYGLTPVSFTFG
                     GIGSKTMINLRASEQRKTIRASYAISNRSYRNRIMATYNTGKMANGWSLSASASYRWA
                     QEGYAQGTFYKGISYYLGIEKELNKNHSLGLTIIGAPTQRGKSNGSVQEVYDLTGNNF
                     YNPNWGYQNGEKRNSRIGSTNKPIGILRHDWKLKENISLINLVSYQQGYESNTRLDWY
                     NGRDPRPDYYKYLPSAALDSSAAVLFTEQWKSNSKWNQVDWDYMYEVNRGKNETVNNV
                     DGIEGNNVTGARSYYIVQDQRTDNRIFNYGSILKTQIGQRWTINGGLNYQYYNANYYS
                     LIDDLLGGEFFLDIDKFAERDLSSNPAAAQPNLDKPNHIARKGDRYGFDYDMNVRNYS
                     LWMQHVFVFPHFDYFISGNIGQTINWREGNVKNGRFPDNSLGESARNKFLEYGLKGGV
                     TYKIDGRNYLTVNGGYLSQAPLVNNSFISSRTRNSIVKNLVNEKVATVEGSYNYVAPN
                     FKLRLTGYLTNFYDKSTRTAFFNDVDGTFVNFNMTGIGQQNGGVELGSEIKLNPQWKI
                     NAVASVGRFVYANNPTVTISNDNDEEIKQLTVYQKGYRVENCPQEAYSVGLSYNSSKF
                     WFITLNANYARMSFLDMNPYRRTSDIANPELVEPGSPAWYKILHEEEFPAAFTLDLFG
                     GKSWKIKKNYINLNVGVNNLLNNTNIKSGGYEQARVYYKLNSTSENGPIDVSKFPPKY
                     FYAYGINYFISLTFRI"
     gene            complement(5814..>6885)
                     /locus_tag="PLG24_06995"
     CDS             complement(5814..>6885)
                     /locus_tag="PLG24_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ24672.1"
                     /translation="HVTPDCVCGLTDMAIRFDGSQYIELGDKTSGLIPTLTGDEFTLV
                     LSLTTSRKYGPMTVFSIVEDCDSYGYELIYNPIQSKFTLNIRSQTELTSINFDDDPGA
                     CWQQVAINRLFNRMDIIVNGQLKYTQKWVTNPDFRSLGLPILGGGGCTGKTTTRFIGL
                     IDEVKVYNRILSVDNAKIVTSKVINKLNDHFQVIFKGQSYGVEFITNCPVNVSWLPTQ
                     GVSDPASNIVELSPETTTKYSVSSFYEGCTDVDTILIKVLDESNIQCNKLLLPNSFTP
                     NDDGLNDTYGISTTYAIDGDCLFEIFNKYGGKVFSTKDPNGRWDGKIGNDVAPPGLYL
                     YKVRYTCADKQYTTSGSVNIIR"
BASE COUNT         1901 a         1399 c         1303 g         2282 t
ORIGIN      
        1 aaggtaaagc cccaaaaaaa ggacttgttg gttgctaagc atcaggaatt attatctttc
       61 actgaccttg ctaaggctgt gaacagacta ctatcaaaaa gtggattttt ctatatcatt
      121 ttgccaggta aagaagcagt tgaatttgag aaaatagctt cttcattttc tattcatcct
      181 gttggaaaat tatttatctc aagttttaaa ggctctggtg taataagaga aataacagtc
      241 taccaaaaag ccgtaatcag cgagattgta aattcggagc atttgttttt atatcgttcc
      301 ggcatgagaa atgattggtc ggaagaatat agacaaatgc tccggaagtt taagaaattt
      361 gattaacaat caattggatg agctttccag aattctcctg gcagcttcaa tgattcttgt
      421 atcttcgagg taaacaatgc cgccttcggg tcccggctca atatgcaggc caactacttc
      481 cccgccacta tcgtaatggt cgccgccaaa ataatttcgc acagtctgct cttcaatgtt
      541 tagttccttt gcgatcttat tgatgcttcc ggttggcaaa ctgtgcttga tacgcctcaa
      601 ttcatcaaat gtaatattca tatgtcaaaa tattttaaaa gaatttgata aataaattaa
      661 acatgacaat ataaaacttt aatctgattg ttcaaaataa tttcatgctt attttcacat
      721 tcgtgtaata attttttttc aattttaatt tatctgaatc aaacctggta tacgttcttg
      781 attataaaaa taaaaaaagc cgattggtat ccattcggct ttttttataa tatctgttaa
      841 atattaattt atgtctgctt tacttctaat atttacctga ggcgtatcaa attgccctac
      901 aattcctgtt acatttacaa ctccggttgg aaaattatcc ttggcaaaag ttgcataaga
      961 agtagtgtac atgtttgctg tacccgttgc atccgtcatt acaacagtac cctcaaacgt
     1021 tgagccactt gatttggaca gtgttacccc aacgactttg atcaatgttg attcataatt
     1081 ttccaaattt gaaactaatt cattcaaggt gattgtcact ggattgatag actttccggt
     1141 accaactacc ttagcattgg cattaggcac gttattgagc tgcaaaagac cattgaattc
     1201 tgatagttcc atgccaccaa ttgcgatttc aacttcatcg cccaggttta gtgtgttatt
     1261 tgctgaaaat cttactacga tacctgcgtt accttcttgc tgtattacaa cattttttga
     1321 agttatatta gagaaagttt tatccgaaat tacaatacct ttgatggatt tagatgcagg
     1381 aaccgtagtt gctgctcctg catataaggc tctgacagcg cttatatcaa ttgtttctcc
     1441 cggattgacc actcccccac taatatcatc tttgcttctt attgtgagtt gattttcgga
     1501 attactgccg cctctggaag caatagccac tatgtcaact gtgcctgtcg ggaaattgtc
     1561 attggcgaaa gttgaatatg aggttgtgta taatgcaata gatccggttg catctttggc
     1621 attgactgta cctgaaaatg ttgttccatt ggatttactt aatgtaacgc ccttaatttt
     1681 gaccaattga gattcatata cttttatatt ggcgttgagg ttgtcgatac tcaattcagt
     1741 tggggtaact gtcttgccgg taccggtcct ggatacattt gtcaatggcg cattgttgat
     1801 ttgcaataaa ccattgaatt cagatagttc caggcccgat attcctattt ccagttcatc
     1861 tcctacattg aagggtactg tttcagtaaa tctcaaagta ataccggcat ttccggattg
     1921 ccacagtacc aaattcttgt tggtgatatt agcatatgtt ttatccgaaa tcacgatccc
     1981 tttgattttt ttgttatccg gaaggctaat cgtagaccct tggtacaatg ctcttacgtc
     2041 actgatgttg atcaattctc cggttacacc gccatcgccg catctttttc cactgaagtc
     2101 agcatcggag gcctccctta ggaataattg cttggtagct ccaaagactg tataaatcgc
     2161 aactatggtt ccattgcctt gaggcatttc caggccggcg aatgttgcat agccggatgt
     2221 ccgcaaggtg attgtcttat tgctacaatc tttaagagtt cggttggttg tagttttatt
     2281 aactgcatct gcataagtcg cacccagatc agcttcaggt acttcgaggc cgtttagttg
     2341 caccagggtg ttgatgtcac catctcccag ctcagcaatg gtcttgacag cgggagtgat
     2401 cggaacatta aattcgccct ttaaaatata ttgattgact accggttctt caattccgcc
     2461 cagcctgtta ttgttagaat catcgaggta ggtaccccct ccaatctgaa aattaccatt
     2521 ataatcccca agggtcagac ctttcagttt aagataaacc ctacggccta caggaaaagt
     2581 attgaaaagc cctacagcat tgatcttgac ttccataccc ccggtactgt cctgaactac
     2641 cagtgtcttg taataattgc cggacttatc atcggctact actaccgcct tgataagata
     2701 gtcttcattg attgtttcaa attttccgtt ggtatgtaat gccctcaggt ctttgatact
     2761 gaagttggca ttgtaggtag gataattagt attatatggc ggctcatcga tgtccgtttt
     2821 tacacaacct aaaaacaagg ttgccatgaa ggctgtaaag aggaaggaat tgataaatct
     2881 tgtcatcttt attttaattg attgaaattt aatattttat attctgaaag taagacttat
     2941 aaagtaattt atcccgtagg catagaagta ttttggtgga aattttgata catctattgg
     3001 tccattttcg cttgttgaat ttaatttgta gtatactctt gcttgttcgt acccacctga
     3061 ttttatgttt gtattattga gcaggttatt aactccaacg ttcaggttta tatagttttt
     3121 cttgattttc catgatttcc cgccaaacaa atcaagcgta aatgctgccg gaaattcttc
     3181 ttcatgaaga attttgtacc atgcaggtga accaggttct accaattccg gattggcgat
     3241 atcgcttgtc cgtctgtatg ggttcatgtc caggaaggac attcgcgcat agttggcatt
     3301 cagggtgata aaccaaaatt tagaagaatt ataacttaga cctactgaat aagcttcctg
     3361 tgggcagttt tcgactctgt accctttttg gtaaacggtc agttgtttga tttcttcatc
     3421 attgtcattg cttattgtaa cagttggatt attagcataa acaaatcgtc caacagacgc
     3481 tacggcattg attttccatt gcggattcaa tttgatttca cttcccaatt ccactccacc
     3541 gttttgttgt ccgattccgg tcatgttgaa attgacaaat gttccgtcca catcattgaa
     3601 gaaagctgtt cgggtggatt tatcgtaaaa atttgtcaga tatcctgtca atcttaactt
     3661 aaagtttgga gctacataat tgtaacttcc ctcaactgtt gctactttct cattgaccag
     3721 attttttacg atggagtttc ttgttctgga agagataaat gagttattga ccaggggtgc
     3781 ttgagagagg taacctccat tgacagttaa atagttacgc ccatcgattt tgtatgtcac
     3841 tccacctttc aatccatatt ccaaaaattt attcctggcg gattcaccca aagaattatc
     3901 cgggaatcgt ccatttttta catttccttc cctccagttg atagtttgac ctatatttcc
     3961 gctgatgaag taatcaaaat gtggaaaaac gaatacatgc tgcatccaca ggctataatt
     4021 cctgacgttc atatcataat caaagccgta tcggtcaccc tttctggcga tatggtttgg
     4081 tttgtcaagg ttgggttgcg ctgcggccgg attggaagaa agatctcttt ctgcaaactt
     4141 atcaatgtct aaaaagaact ctcctcctaa tagatcatca atcaaactgt aataatttgc
     4201 attataatac tgataattca aaccgccatt tatcgtccac ctctgcccta tctgagtttt
     4261 taggatggaa ccataattaa aaatccggtt atcggtccgt tgatcctgta ctatgtagta
     4321 agagcgggct ccggtaacat tatttccctc aattccatca acattgttga ctgtttcgtt
     4381 tttacctctg ttgacttcat acatataatc ccagtcaact tgattccatt tgctgtttga
     4441 tttccattgc tctgtgaata aaacagcagc agaagagtca agagcagcgc tggggaggta
     4501 tttataataa tcaggtcttg gatctcgtcc gttgtaccaa tcgagtcggg tattggattc
     4561 gtagccttgt tggtacgaaa caaggttgat cagggaaata ttttctttca atttccaatc
     4621 atgtctcaga atgcctatcg gcttattggt tgaacctatc cggctgtttc gtttttcacc
     4681 attttgatat ccccaatttg gattataaaa attattcccg gtcaggtcgt acacttcttg
     4741 tacagatccg ttgcttttac ctctttgggt tggcgcacct atgatcgtca atcctaatga
     4801 gtgattttta ttgagctctt tctcaatgcc tagataataa gagataccct tataaaaggt
     4861 accttgggca tatccttcct gggcccacct gtatgatgca ctagcagaaa gagaccatcc
     4921 attggccatc ttgcctgtat tgtatgtagc cataatcctg ttgcgataac tgcggttaga
     4981 gatcgcgtag gaagccctga ttgtcttacg ttgttcagaa gctctaaggt tgatcatggt
     5041 tttggaaccg atgcctccaa aagtgaagga taccggagta agaccataat tggcatctgt
     5101 atttctcaac acatcgttca aaccacccca ttctccaaat acgagagctc cgttttccgg
     5161 atcgttcatt tgaataccgt tcaagtaaac tgaattctgt tctgtgtcat atccccgaag
     5221 tctaaaccgg aatacgccca aattataacc tgcaattgaa agaaaaggat cccgggaggc
     5281 tgacaacagg ctgctaattt cctgtgaacc atcgtttgca ctttcatcat caatggatat
     5341 taccggtatc gtcccggtac tttgaactct tgcattgtat gcggcatctc tatacatggt
     5401 tataattacg ggaactgccg cattgacgtc agcaggttgg atagcgtaat ctacatcgat
     5461 gaaaccttct ttcttgatat gtatatttcc ggagaagtta ccggtgtatt caattttgaa
     5521 aaagccaact ttgtcggtag ttgtaccaat gcccagggga tgtagcatta ccatggcgtc
     5581 ctctacagga ttggaattat ccgcttcttt caggatgccc tggaaaacga atacactttg
     5641 attataggct ttaaaggaag aaaataaaaa ggtgagtatg aagaatacaa ctaaattaaa
     5701 tctcagcata aactctatat atattacaaa tgtaaaagag tttatgtaat ttttctacct
     5761 ttttttatca tattaacctt gtatattggg atgcgaatgt tatccacaat ctatcatcta
     5821 ataatattta cactccctga agttgtatat tgtttatctg cacaggtata gcgtactttg
     5881 tataaatata agccgggtgg agccacatcg ttgcctattt taccatccca ccggccgttg
     5941 ggatctttcg tggagaatac cttgcctcca tacttgttga atatttcgaa aaggcaatca
     6001 ccatcaatag cataagttgt agatatccca taggtgtcat tcagcccatc atcgtttggg
     6061 gtaaagctat ttggcaatag tagcttgtta cattggatat tagattcgtc aagtaccttt
     6121 attagaatag tatccacgtc agtacaaccc tcataaaaag aagatacgga gtattttgtt
     6181 gttgtttcag gtgataattc aacgatattg gaggccggat ctgatacgcc ctgagttggg
     6241 agccatgaga cattaaccgg acaatttgtg ataaattcta ctccatagga ttgtcctttg
     6301 aaaattacct gaaaatggtc atttagttta ttgattacct tagaagtaac aatttttgca
     6361 ttgtcaacgc ttagtattct gttatatacc tttacttcat cgatcaaacc gatgaatctt
     6421 gtagttgtct taccggtgca accgccacca cccaagatgg gaaggcccag tgagcggaaa
     6481 tcgggattag tgacccattt ttgggtatat tttaactgcc cgttgacgat gatatccatt
     6541 ctgttgaaaa gtctgttgat ggctacctgt tgccaacaag ctcccggatc atcatcaaaa
     6601 ttgatggaag tcaattctgt ctgagatcgt atattgagtg tgaacttact ctggataggg
     6661 ttataaatca attcatatcc ataactatca cagtcctcta ctatggaaaa cactgtcata
     6721 ggaccgtact tcctagaggt tgtcaaagag agtaccagcg taaattcgtc tccggtcaat
     6781 gttggaatca atccacttgt tttatcccct aactcaatat actgtgaacc atcaaaacga
     6841 atcgccatgt ctgtcaggcc acatacacaa tccggagtta catgt
//
LOCUS       DAOQQN010000155         6853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4623_length_6853_cov_5.839659, whole genome shotgun sequence.
ACCESSION   DAOQQN010000155 DAOQQN010000000
VERSION     DAOQQN010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4623_length_6853_cov_5.839659"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..608
                     /locus_tag="PLG24_07000"
     CDS             66..608
                     /locus_tag="PLG24_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ24673.1"
                     /translation="MSIDLQQPLENDFVGLVPLQVGDFELLYLVASNPKVWEQHPNKD
                     RWKRDVFLTFFQGAMESGGAYRIVNKSDGKTIGSTRFYGHDPDDESIMIGYTFYDPEY
                     WGKGYNPAVKILMMDYIFRFVSKVFFHVGAVNKRSRIAIERLGAVKVGEKEIEYFGEK
                     AAVNIIYCIEKENFLSRYHH"
     gene            complement(614..1636)
                     /locus_tag="PLG24_07005"
     CDS             complement(614..1636)
                     /locus_tag="PLG24_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HMZ24674.1"
                     /translation="MGQRIFPAEWARQSGVAFTFPHANSDWGPYLEIVIPTFVEIITL
                     VSRHEKVLVVCQDAELVSTLLKDAVQENLILVELPSNDTWARDHSAITILEEGHPSLL
                     DFIFNGWGLKFPANHDNLITKRLQAKGIFGDLAVHTYGLAIEGGALESDGTGTLLTTS
                     ECLLSPNRNPHLNREMTENTLREILGVNRFLWLDHGYLAGDDTDSHIDTLARFCSEDT
                     IVYVSCDDKEDEHYESLKAMECQLKTFARSDGSPYNLVPLPMAPARFHPEGYRLPATY
                     ANFLIINGAVLLPVYGDPKQDDMAVRVIQKCFPNRKIHPVDCNSLILQHGSLHCITMQ
                     FPEGVL"
     gene            complement(1810..5295)
                     /locus_tag="PLG24_07010"
     CDS             complement(1810..5295)
                     /locus_tag="PLG24_07010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ24675.1"
                     /translation="MKNILLSVLLIWSATEVYSQCSVDAVNPDLLNQTVCEGESIIPI
                     IINITGSFNVDGLSGTGLQTSFSQNNSQLTIFGTPTVDLINYTVSCSWIGFTGPSGTS
                     VSGTITRVPLPTVDQPSNIFECGGTQVTVIFSGSNATSYSWTNSNTSIGLGSSGSGNI
                     NFVASNVNGTQTATITVTPSNTSCSGSPKTFTITIYPSPDISGNNWVCEGNQEQLTGS
                     GMPAQSNPWTSSNPDVATVDNNGLVTGNVPGTVIITYTALNGCFDQIGFTVYPKAYAG
                     IDQNILCWHLEYAYMAASGTGQWELLTTDPPGLNLNIIDPNDPNTGVNNFSGPGTYIL
                     RWTNEFGCFDEAIITAQDICPCENPPEIILSIDEKSVCGAQAITVDSNIFLNASTLSF
                     FHNGSGTLEHLVFPGNSFMFTYTPTPSDFGKTIQITIVTDDNDGSGPCEPATAIFFLH
                     IDGNPEVGPDIQVDCASRDSAIISATSGEGLWILIDTDPPGMTLDIVDPSNVKTTIKN
                     FSGTGYYTLIRQLTSGCSDTLTVEAKNDCDCTAQPDLNLSATSFTTCDLKTISVPNNT
                     YFNATTVLVSHNGSGNASVTHTSSNTFTFSYTPTQADTGKTITLTLYTDDPDMNGPCY
                     ADTMKFVLQYHLIPHVSSDVAEVCTKSGGVILSASGSGNWNLVSTTPAGLTVSISEPT
                     SANTLVTSFSGPGSYILRWTTSEGCANDLAITAVDCPCAPDIVLSTEGAYCGQNNGLI
                     HVKVSNATYPYSCSLSGAKNKTIDHVSTDTVIFDHLGPGTYKVSVIDAAGCQSASVTQ
                     VHDAGNTLSLILGSVKHSFCGDHNGSFSAYVTGGRPPYTFTVNGSMVSYPVSMDNLSA
                     GSYMLSVVDNAGCTKDTTITLLESNPFTANLTDSGFDCNSATGIISVTAEGGRPPYAY
                     ALNHGVYGENPVFTGLTPDVYTISVVDVDLCYTELTITPFIKADFSAVDDYLQWHFQY
                     NPLIIDVISNDSITNPADYSVRPLSEIITDDDGNEIGKLFNREVGDQRMEFYFDGARQ
                     NLYPELIKMPYLLCETDCPVKCDTGIIYLLQKVPCTDDNGGFVNTITPNGDGRNDVLI
                     IPNFPKCNVTRMAIRIFNRWGNAVYENSEYGNEWDGKNNDGSPLPEGTYYYVLSLKTN
                     DGKDYVIKNFVEIIR"
     gene            complement(6312..6467)
                     /locus_tag="PLG24_07015"
     CDS             complement(6312..6467)
                     /locus_tag="PLG24_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24676.1"
                     /translation="MRRTPVIPQIKGGDPHPVTQPPGNGLPIIKSAEKTVQDDNIRAT
                     VAVLLEI"
     gene            6408..>6853
                     /locus_tag="PLG24_07020"
     CDS             6408..>6853
                     /locus_tag="PLG24_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMZ24677.1"
                     /translation="MSDRMRVTTFDLRNHGRSAHVPDFSIELMASDVYEMLQALHIGS
                     AVIIGHSMGARVAMCFSLAYPAVTDGLIAVDMATKKYERGHDDIFAALEAVDLNAGSR
                     KEVENILAKRIPDEGTLQFLMKNLSRREGGIGFEWKMNLPVIKANY"
BASE COUNT         1870 a         1498 c         1570 g         1915 t
ORIGIN      
        1 aatggaaaat tacagggcac cacaagtaga aaaatggata acacccgaca aaacctgaac
       61 cggtcatgag catagatttg caacaacctc tggaaaatga ctttgtcggc cttgtacccc
      121 tacaggtagg cgatttcgaa ttgctctatc tggtagcctc caatccgaag gtgtgggagc
      181 aacatcccaa caaagatcga tggaaacgag atgtctttct gactttcttc cagggagcta
      241 tggaaagcgg tggagcatac agaattgtca ataaatccga tggcaaaacc ataggtagca
      301 cacgatttta tggtcatgat ccggatgatg agtccatcat gattggttat accttctatg
      361 accccgaata ttgggggaag ggttataatc ctgccgttaa gatcctgatg atggactata
      421 tcttcagatt cgtatcaaaa gtgtttttcc atgtcggtgc ggtcaataag cgatcccgga
      481 ttgccatcga aagacttggg gccgtcaagg tcggcgaaaa ggaaattgaa tatttcggcg
      541 aaaaagctgc cgtcaacatt atctattgca ttgaaaagga aaatttcctg tcaaggtatc
      601 accattaagg tagttaaagg acgccttccg gaaattgcat ggtaatgcaa tgcagtgaac
      661 catgttgcag gatcagtgaa ttacaatcca ccggatgaat tttcctgttc gggaaacatt
      721 tttgtattac tcttaccgcc atgtcgtcct gtttcggatc gccatatact ggtaaaagta
      781 cggcaccatt gatgatcagg aagttggcat aggtcgcagg aagcctgtac ccttccggat
      841 gaaaacgggc aggggccata ggcaatggaa cgaggttata agggctgcca tcggaacggg
      901 caaatgtctt cagctggcac tccatagcct tcaatgactc atagtgttca tcttccttat
      961 catcacacga cacatagaca atggtatctt ctgaacagaa tctggcgagc gtgtcgatat
     1021 gtgagtcggt atcatcaccg gccagataac catggtcgag ccataagaat ctattgacac
     1081 caagaatttc cctcagggta ttttctgtca tctcacggtt cagatgagga ttccggttag
     1141 ggcttaggag acattctgag gtggtcagga gagtgcctgt cccatcgctc tccaaagctc
     1201 cgccttcgat ggccagtcca taggtatgca ctgccaggtc gccaaatatt cctttggcct
     1261 gcaatctttt ggtaatgagg ttgtcgtgat tggccggaaa cttcagaccc cagccattaa
     1321 agatgaagtc aagcaaggaa ggatgcccct cctccaggat ggtaatcgca ctgtgatccc
     1381 tggcccaggt atcattggag ggaagttcga caagtataag attctcttga accgcatctt
     1441 tcaacagggt gctgaccaac tcggcatcct ggcatacaac aaggactttt tcatgccggg
     1501 agaccagcgt gatgatctca acaaatgtcg ggattactat ttccagatat ggcccccaat
     1561 cactgttggc atgagggaag gtaaatgcca cgccactttg acgtgcccat tcagcaggaa
     1621 atattctttg tcccatttat tacgattatt gccacaaaaa taggaatatg cagcgaagtg
     1681 caatgaattt aaaggatttc taagcacaca ataacaatga ggaattatat ctaccacatc
     1741 caccccatgc ccaccacaat acgggttaag tttaacacac cgcttgacaa tactagcttt
     1801 tccttccctt tatcttatta tttcaacgaa attcttgatc acataatcct tgccatcgtt
     1861 ggttttcaaa gacaatacat aataataggt cccttccgga agtggcgaac cgtcattatt
     1921 ttttccatcc cattcattgc cgtattctga gttttcatat acagcattac cccagcgatt
     1981 gaatattctg attgccattc gtgtaacatt gcacttggga aaattcggta ttatcaacac
     2041 atcatttctg ccatcgccat tcggagttat ggtattgaca aaaccaccgt tgtcgtccgt
     2101 acaaggaacc ttctgcaata gatatataat tcctgtatcg catttgaccg ggcaatccgt
     2161 ctcacacaac aggtatggca tctttatcaa ttccgggtaa agattttgtc gggctccatc
     2221 aaaataaaat tccattcgct ggtcccccac ctcacggtta aaaagcttgc ctatctcatt
     2281 cccatcatca tctgtaatga tttctgataa tggtctgact gaataatctg ccggattggt
     2341 tatactatcg ttagatatga catcaataat cagcggattg tactggaaat gccattgcag
     2401 gtaatcatcg actgctgaaa agtccgcctt gataaaaggc gtaattgtca actcagtata
     2461 acagagatcc acatctacca ctgagatggt gtatacatcc ggtgttaaac ctgtaaagac
     2521 tggattttct ccatatactc catggttcag ggcataggca tatggaggcc ttcctccttc
     2581 ggctgtaact gaaatgatgc ccgtggcact gttgcaatca aagcccgaat ctgtcagatt
     2641 agccgtaaac ggattactct ccagaagggt tatcgtagta tctttggtac aaccggcatt
     2701 atcaacgaca ctcaacatat aactgccagc actcaaattg tccattgaaa ccggataaga
     2761 gaccatgctg ccattgactg tgaaggtata cggcggtcgc cctcctgtaa cataagcgct
     2821 gaaactaccg ttgtgatctc cacaaaatga atgcttcact gacccaagga taagacttaa
     2881 ggtattaccg gcatcgtgga cctgtgtgac agaggctgat tgacatccgg cagcgtcgat
     2941 gactgaaacc ttatatgttc cagggcctaa atgatcgaaa attactgtat cggtactgac
     3001 atggtcgatg gtcttgttct ttgcccctga caatgaacag ctatatggat aggtagcatt
     3061 actcaccttg acatggatca ggccattgtt ctgtccgcaa tatgcccctt ccgttgacag
     3121 aacaatgtct ggtgcgcatg ggcaatcgac tgctgtgatt gccaggtcat tggcacaacc
     3181 ctccgatgtt gtccaacgaa ggatataact tcccggtccg gaaaaggatg tcaccagcgt
     3241 atttgctgat gtaggctcag atatactgac agttagacct gctggcgttg ttgaaaccag
     3301 attccagttg cccgaaccgg aagcagatag gataactcct ccggattttg tacagacctc
     3361 cgccacatcg gaagaaacat gaggaatgag atgatattgt agcacaaatt tcatggtatc
     3421 cgcatagcat gggccattca tgtccgggtc atcagtatat agggtcaggg tgatggtttt
     3481 gccggtatca gcttgtgtcg gagtataact gaaggtaaat gtatttgaag aagtgtgagt
     3541 aaccgaggca ttaccacttc cattgtggct gacaagtacc gtagtcgcat tgaaatatgt
     3601 gttgttgggg acggagatgg ttttcaggtc acaagtggtg aatgatgttg cactgagatt
     3661 cagatcgggc tgagccgtac agtcacaatc attctttgcc tcgactgtga gggtatcact
     3721 acaccctgag gttaactgtc tgataagagt ataatatccg gtgccggaga agttctttat
     3781 ggtagtcttg acatttgagg ggtcgactat gtcaagtgtc atgcctggtg gatcggtatc
     3841 gataagaatc caaagacctt ctccactcgt ggcactgatg atggcactgt cccgggaagc
     3901 gcagtcaacc tgtatatcag gtccgacttc gggatttccg tcaatgtgca gaaaaaaaat
     3961 ggcagtcgca ggctcacagg gaccgcttcc atcattgtcg tctgtcacga tggtgatttg
     4021 aatggtttta ccaaaatctg aaggggtggg tgtgtaagta aacatgaatg aattgccggg
     4081 aaagacaaga tgttccagcg ttccgcttcc gttgtgaaag aaagaaaggg tactggcatt
     4141 taaaaagata ttgctgtcta ccgttatggc ttgagccccg caaacactct tttcatcaat
     4201 gcttaatatt atttccggcg ggttttcaca agggcagata tcctgtgccg tgatgatagc
     4261 ctcgtcaaaa cacccgaatt cattggtcca tcttaatata taggtcccgg ggccggaaaa
     4321 gttgttaacc cctgtattgg gatcattcgg atcaataata tttaagttca gacccggcgg
     4381 atcagtagtt agaagttccc attgtcctgt gcctgatgct gccatatagg cgtattctag
     4441 gtgccagcaa agtatattct gatcgatacc ggcataggcc tttgggtaaa cagtaaagcc
     4501 gatctggtca aaacagccgt tgagcgcagt ataggtaatg ataaccgtgc ccggaacatt
     4561 tcccgtgacg agtccattat tgtccaccgt tgcgacatca ggatttgatg aggtccaggg
     4621 attactttgg gctggcatac cactacctgt aagttgttct tgattccctt cacacaccca
     4681 attattacca ctgatgtctg gacttggata gatggtgatg gtaaatgttt ttggactgcc
     4741 ggagcagctg gtattggatg gggtgacggt gatggtggct gtctgtgtac cgtttacatt
     4801 tgacgccacg aagttgatat tgccactgcc ggaggagcca agccctatac tggtattgga
     4861 attggtccag gaatacgaag tggcattact tccgctgaaa ataaccgtta cttgtgtgcc
     4921 cccacactca aagatgttgg agggttggtc gaccgtgggt agaggtaccc gggtgatagt
     4981 cccgcttacc gatgtgcctg aaggcccggt aaagccaatc caggaacaag aaaccgtata
     5041 attaatcaag tcaacggttg gtgttccaaa aatagtcaac tggctgttat tttgagaaaa
     5101 tgacgtttgc aaccctgttc ctgataagcc atccacattg aaggagccgg tgatattaat
     5161 tatgatagga attatagact ccccttcaca aacagtctgg tttaaaagat ccgggttgac
     5221 cgcatcaaca gagcactggg aatagacttc tgttgccgac catattagaa gtacacttaa
     5281 aagtatgttt ttcatagtgg tgaattttga atgatttatg ccaaatcaat gacctcaaaa
     5341 gacaacttgt tactaatcat tagttttccg gtcttacaat tcgttcgtta tccaatcctt
     5401 gcaggttaca tactttcttg tcttcctacg agccggagcc gctcttcctt ttatgaagcc
     5461 tgccaaacta gtgatgccta tgggtgataa aacccctatc cacatgctac gatttgtgtt
     5521 tacaaattta gtgaaatctc gataaaaaag cagattaatt atctgaaatg tttcagtctt
     5581 aggatgttaa taccaaaaat ttatggacta ttatataaat catcttggta taatttcttc
     5641 aactcggttc cagatcctgc aaatagaccg tattttactg ttatgacact gagtcgtcga
     5701 cttttgaaca ggctactctg atcctatctt tttgttggtc ctgtgtttcc taggatggtt
     5761 ttaaacattt accttaatca ggattagtaa tcagggcttc ataggtttaa cggccaaagg
     5821 aggtaatgaa ggattcttgt gtgacttgat tggaggagag agtggccggg atgggctgag
     5881 ctggaaatcc ctggctttta caccatacaa caaataaata tttttatcca attaccttag
     5941 ggtaatcata aaatatggga gtgtgtgctt cccggacttt tgttactgaa tcctctttgg
     6001 tctatcccta aggaccaaag aaatttgact tgcagatagc attgatttat tggcaacatt
     6061 caaaaacaat aagcgcaaaa tcgccctcct gtcttatctg tccctgtata aattaaaaaa
     6121 ccaaacatca gcgcctctga gaaagccatg ggtttatttt aatgatcaaa acgcgctggt
     6181 cttcaaagaa gcctcgcctg ctctcaacac cttaatgcct ctttctcccc tctatcagtt
     6241 tccttgttta ctcggaaatt aatgttttag tatctttgtc gggcaaccaa atcaatattt
     6301 gacctatcga actaaatttc aagcaatacg gcgacagtgg cccggatgtt atcatcctgc
     6361 acggtctttt cggcactctt gataattggc agaccattgc ccggaggttg agtgaccgga
     6421 tgcgggtcac cacctttgat ttgaggaatc acgggcgttc ggcgcatgtt cctgattttt
     6481 cgattgagct gatggcttcg gatgtgtatg agatgctgca agccctgcat attggttctg
     6541 ccgtcatcat agggcattcc atgggcgcga gggtagccat gtgtttcagc cttgcgtatc
     6601 cggcagtgac cgatggcttg attgctgtgg atatggcaac caaaaaatac gaaaggggac
     6661 atgacgacat ctttgccgca ttagaggctg ttgatttgaa cgccgggtcc aggaaagagg
     6721 tagaaaacat tttagcaaaa cgtattcctg acgagggcac tcttcaattc ctgatgaaaa
     6781 atttgagccg aagagaagga ggtatcggct ttgaatggaa aatgaacctg ccggtaatta
     6841 aagctaatta cga
//
LOCUS       DAOQQN010000156         6851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4624_length_6851_cov_5.146410, whole genome shotgun sequence.
ACCESSION   DAOQQN010000156 DAOQQN010000000
VERSION     DAOQQN010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4624_length_6851_cov_5.146410"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="PLG24_07025"
     CDS             complement(<1..1144)
                     /locus_tag="PLG24_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HMZ24678.1"
                     /translation="MKKKFDASIGRIRDTETAYTDYSKCAYPGLEPFLRGPYASMYVK
                     RPWTIRQYAGFSSAEESNAFYKRNLAAGQMGLSVAFDLATHRGYDSDHPRVVGDVGKA
                     GVAIDSVEDMKVLFDGIPLDKMSVSMTMNGAVLPVMAFYIVAAEEQGVSTAQLSGTIQ
                     NDILKEFLVRNTYIYPPRPSMRIVSDIFAYTARHMPKFNSISISGYHMQEAGASAPIE
                     LAYTLADGLEYIRTGIAAGLDIDSFAPRLSFFWGIGMNFYEEIAKMRAARMLWAEIVS
                     GFNPKNNKSLMLRAHCQTSGWSLLAQDPYNNITRTAIEAMAAVLGGTQSLHTNALDEA
                     LALPGDFSAKIARDTQLYLQHETDITRAIDMLGGSEYLERLTSEITA"
     gene            1311..2306
                     /locus_tag="PLG24_07030"
     CDS             1311..2306
                     /locus_tag="PLG24_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HMZ24679.1"
                     /translation="MNGFGRIFRISIYGESHGSEAGILIDGCPPGIPVDEQLLLKDLE
                     RRKPGAKGTTPRVESDYPMILSGVFNGYSTGAPIAIRFENQNTRSEAYEKQRDIPRPG
                     HADFTAHTKYKGYEDFRGSGHFSGRLTVGIVAAGALAKKVTSHVLGKEITYVAEILEV
                     GGMKDIEAGIQKAIQAKDSIGGIIECRIGGLPVGLGEPFFDSVESMISHLAFSIPAVK
                     GIEFGAGFGAAQMFGSDHNDAILDASGTTATNHAGGIAGGITNGNEVVFRVVIKPTSS
                     TPKEQTSYNWETGRIETFSYKGRHDLCIALRVPVIMEAVAAIAIADLALINKAYQ"
     gene            complement(2683..3234)
                     /locus_tag="PLG24_07035"
     CDS             complement(2683..3234)
                     /locus_tag="PLG24_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24680.1"
                     /translation="MRFYFFSIALIFTLLSSCSNDNTRDKDNKQVDVTTPTPVDTPAA
                     KPFRLTSEHYPEYFSFEKEEKGFDPCELLTMNDITVTCKPTAAVTINNRNRDKERVCA
                     YHWMGTDSTLCEVRFSVMTYSMDHDKLVQLFKPDANGIIEHTDWGVRWLVGNKVLMVT
                     YTGELIKKVNILTIKDHVRPFRK"
     gene            complement(3394..4125)
                     /locus_tag="PLG24_07040"
     CDS             complement(3394..4125)
                     /locus_tag="PLG24_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HMZ24681.1"
                     /translation="MKRELHAWHSPALNKTMEIAIYGHFGMPLLLVPTAAADYLEYER
                     FLMIDVIKPFIDSGKVKVFSINSINSESWLNNKMHPRHKSIRHVQFNNYVYDEVVPFI
                     KSQTSEESKIIISGASLGALHSANLYFKRPDLIAGVIAMSGDYDLTSYTDGYYDDDVY
                     FNSPMAYMPGLTDEAYLRRMRNNKHIHILTGSGNYETPDASRRFAAILQSKGIPVDLD
                     VWGYDMHHDWPTWRAMLPYVLETKF"
     gene            4317..4739
                     /locus_tag="PLG24_07045"
     CDS             4317..4739
                     /locus_tag="PLG24_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24682.1"
                     /translation="MFRLFIAMFFFVAYSYGCVQPDSNVEVTPKMITGTWILDSALRD
                     GTPTESLDGLFYTFEQKKVVSNITGDTNETMFEIQGSKIVHLNDDSMVYQIETLNDSI
                     LKVSSNIHNIPFELKFRKFDSTTSTDEHGDLQESDGLK"
     gene            4756..6213
                     /locus_tag="PLG24_07050"
     CDS             4756..6213
                     /locus_tag="PLG24_07050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HMZ24683.1"
                     /translation="MFRWLALVVFFTTALSVQALNVSILISQYYSIDKSLEPYLEVYL
                     HTIASTVDPLPSSSGDGLMEKKIKVLILFKQNNDIVNYDNFIIESGRKKDIEDFVTLK
                     RYGMKPGEYNIEFTLADLANTNNEYRDSVTVRINSAPEHPFLSNLQLVADTTSSADEH
                     IAKNGIFYELIPYGYMSPAINRFGLYFESYNTDQLATDIVNYHVKISKRINNTYKLVG
                     RTALKRRSARIDDILAWFDVTYYESGQYNIMVYLSDKDRNAIDSAEYYFTKSNPVRDE
                     AILSKKFMDEVETSFAGKLSRDQLNYTLRAIAMNVINSDVELVNRLIKDDNVIAKSNY
                     IYKYFKEKSALHPEEYYTQYMEVAKAVDKKYKTGFGYGFESDRGLIFMKYGKPSDMIS
                     ITDDPSCAPYEIWQYNEVPKLMQSNVKFLFFNPFLDGMDYRLLQSNARGEMRNPNWKK
                     ELYKTVATNPENQASERYEVPKGFNRQAEEWLQDL"
     gene            6254..>6851
                     /locus_tag="PLG24_07055"
     CDS             6254..>6851
                     /locus_tag="PLG24_07055"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HMZ24684.1"
                     /translation="MKFKVFSTLCVILLLLSCKREPKPISVDIPVSDVPKIDYRVTGI
                     IPHNTSFFTEGLLIYNGKFLESTGSPEELENVESIVATVDISDGKTEEKIRLDKTRYF
                     GEGIVVLHDKLYQITYKNQKGFIYDAKTFKKLGEFGYPSKEGWGMTTDGTHVIMSDGT
                     DMLSFLLPPEMKVVKTIRVTQNDISVRNVNELEWIKGYI"
BASE COUNT         2073 a         1491 c         1432 g         1855 t
ORIGIN      
        1 gagcagttat ctcggaagtg agccgttcga ggtattctga accgccaagc atatcgattg
       61 ccctggtaat atcggtttca tgttgcagat acaactgagt atccctggct atctttgcac
      121 taaaatctcc gggcaatgcc agtgcctcat ccaacgcatt ggtatgcagg gattgggtac
      181 ctcccaatac agctgccatg gcttcaatcg ctgtacgggt gatgttgttg tacggatcct
      241 gcgccagcaa actccatcca ctcgtctggc aatgcgccct caacatcagt gatttgttgt
      301 tcttcgggtt gaaaccactg acgatttcgg cccaaagcat ccgtgcggca cgcatcttgg
      361 cgatttcttc ataaaaattc atgcctatgc cccaaaaaaa agaaagcctc ggtgcaaaac
      421 tatcgatgtc aagacctgca gcgataccgg tccgtatata ttccaaccca tctgccaggg
      481 tatatgccaa ctctatcggt gcactggcgc cggcctcttg catgtggtag ccactgatgc
      541 tgattgaatt gaatttaggc atgtgtctgg ccgtataggc gaatatatcc gacacgatcc
      601 gcatggatgg tcgcggtgga taaatgtagg tatttcgcac cagaaactct ttcaggatat
      661 cattctgtat tgtgcctgac aattgagctg tgctaacacc ctgctcttca gcagctacaa
      721 tatagaacgc cataacaggc aatacagcgc cattcatagt catggagact gacattttat
      781 cgagtggaat accgtcaaac agcactttca tatcctctac ggaatcaata gcaacgccag
      841 ccttgcccac atcgcccact acacgaggat ggtcgctatc atatccccta tgtgtggcca
      901 ggtcaaaagc aacgctaagc cccatctgac ctgctgcgag attccgctta tagaaggcat
      961 tactttcttc agcagaggaa aagccggcat attggcgtat tgtccagggc ctcttgacat
     1021 acatagaggc gtaaggtcca cgtaaaaatg gttccaaacc gggataagca catttactgt
     1081 agtcagtata agctgtctca gtatccctta tccggccaat ggatgcatcg aatttttttt
     1141 tcaaaataaa aacatttctg aagcgtaaag atagggaata tttggattgg aagggcgcta
     1201 aggctctatc cgtgtaaagt caagtttctc aaagactgaa ccaacaaatt gcgcagcctg
     1261 ctttttcatc ctatctccat tttgaccgaa ctttttctca tctttgtgcc atgaacggtt
     1321 ttggcagaat attcagaata tccatctatg gcgagtcaca cgggtcggaa gcaggtatcc
     1381 tcattgacgg atgcccaccc ggaatacctg tggatgaaca attgttacta aaggatcttg
     1441 aacggagaaa accgggcgcc aaaggcacta caccaagggt ggaatcagat taccccatga
     1501 ttttgagtgg tgtattcaat ggttattcaa ccggtgcgcc aatcgccatt cggtttgaaa
     1561 accaaaatac aaggagcgaa gcatacgaaa aacaaaggga tattccacga ccgggacacg
     1621 ctgatttcac cgctcatacc aagtacaagg gatacgagga tttcaggggt agcgggcact
     1681 tctcgggaag attgaccgta ggcattgtgg cagcaggtgc attggccaag aaagtcacat
     1741 ctcatgtcct gggtaaggaa attacttacg tagctgaaat ccttgaagtg ggtggtatga
     1801 aggatataga agccggcatc caaaaggcta tccaagccaa agattctatc gggggcatca
     1861 tagaatgcag gataggcggg ctgcctgtgg gattgggaga accattcttc gattcggtcg
     1921 aatccatgat ttcgcatctc gccttttcca ttccggcagt gaagggaata gaatttggcg
     1981 ccggatttgg tgccgcacaa atgttcggtt cagatcacaa cgatgcgatc ctggatgcat
     2041 cgggaactac tgccactaac catgcaggcg gaatcgcagg cggaatcacc aatggaaatg
     2101 aagtagtatt cagggttgtt ataaaaccaa cctcttcaac accaaaggaa caaacatcct
     2161 acaattggga aaccggacgg attgaaactt tttcctacaa aggcaggcat gatctttgca
     2221 tcgctctaag ggtacctgtc atcatggaag ctgtagctgc cattgccata gccgatctcg
     2281 ccttgatcaa caaggcttat caataaatca ggattctgca aactacaatc gatcttatgc
     2341 tgatattgta ttttcagagc aacaatcagg cggaaattaa gctataaatc cttagaaaat
     2401 caccaagagg tgttaaagcc tgtaataccg aagatacatt cgttatagac caatttcatt
     2461 gatctatacg actgtaacct cggcatcccc gaatcgctta gctctcggga caagtatacc
     2521 aaaaatttat ggactattat ataaatcatc ttggtataac tcattcgtcc cggaaaaggt
     2581 gcgcaatgct ccaactcttc ctggttctac ctttccccga acagactgcc tgtttaccgg
     2641 accagttaaa ctttggttct gtgattgaat tctacaaacc aattactttc tgaaaggcct
     2701 tacatgatcc ttaatcgtaa gaatattcac ctttttaatc agttcaccgg tataggtaac
     2761 catcaatact ttattgccaa caagccatcg cactccccag tctgtgtgtt cgatgatacc
     2821 attggcatcc ggcttgaaca attgtactaa cttatcatgg tccattgaat aggtcataac
     2881 agagaaacga acctcacaca gtgtgctatc tgtgcccatc caatgataag cgcataccct
     2941 ttccttgtct ctattcctgt tgttaatggt caccgctgct gtcggcttgc aggtaacagt
     3001 gatatcattc atggtcagca actcacatgg atcaaatcct ttttcctctt tttcaaagga
     3061 aaaatactcc ggatagtgtt ctgaagttag cctgaacggt ttggcagcag gagtgtctac
     3121 cggtgttggt gtagtaacat ccacttgctt attgtcttta tccctggtat tatcattgct
     3181 acatgatgat aacaaagtga atatcagagc tattgaaaaa aaataaaatc tcatacagaa
     3241 ttaatcactg atacaaaaat cgtattgtac ttgaagcaga acctacacat ttgaaggcat
     3301 gtcaatatgt gcgtaccgat tcctaaatcc tacttcgtca tgcatatggt ttgcgattat
     3361 cctttatgca ccaaggatgg ttgataccaa aaatcagaat ttagtctcaa gtacataagg
     3421 cagcatggct ctccaggtag gccagtcgtg gtgcatatca taaccccaga catccagatc
     3481 gaccggaatc cctttagatt gcaggattgc tgcaaagcga cgggaagcat ccggggtttc
     3541 atagttacca cttccggtca gaatatggat atgcttgtta ttcctcattc tgcgcagata
     3601 ggcctcatcg gtcaggccgg gcatataggc cattgggcta ttgaaataca cgtcatcatc
     3661 atagtaacca tccgtgtagg aagtcagatc atagtcacca ctcatggcta tcactcctgc
     3721 aatcaggtca ggtcgcttaa aataaagatt ggcactatga agagcgccca acgatgcacc
     3781 ggatataatg atctttgact cttcagatgt ctgactctta atgaatggaa caacttcgtc
     3841 gtagacgtaa ttattaaact gcacgtgccg gattgatttg tgtctggggt gcatcttatt
     3901 gttgagccag ctctcggaat taatactgtt gatggaaaaa actttaactt ttccactatc
     3961 aatgaagggt ttgataacat caatcatcaa aaatctctca tactccaaat aatctgcggc
     4021 cgcggtaggc acaagcaata aaggcatacc aaaatggcca tatatggcga tctccatggt
     4081 tttgttgagc gccgggctat gccatgcgtg caattctctt ttcatccgta actatgtttt
     4141 ttaattgaat ttatttagcc aaagataaaa gaaagtgcat tgattgggca agattaaccg
     4201 acaaattttg aacactttgg ttttttttat gtattactga ttgagactaa atcacaaagc
     4261 gatgtttcaa gtccccaaaa atatttattt ttgtagttta aaccaatttg aattttatgt
     4321 ttaggctatt tattgccatg ttcttctttg tagcttactc ttatggctgt gtgcagcccg
     4381 attctaacgt ggaggttaca ccaaagatga taacaggaac atggatactt gactcagcac
     4441 tcagagacgg cactcctacc gagtcattgg atggtctttt ttatactttt gaacaaaaaa
     4501 aagttgtgag caatattacc ggcgacacca atgaaacaat gtttgaaatt cagggaagca
     4561 aaatagtaca tttaaatgac gacagcatgg tgtatcaaat tgaaaccctg aatgacagta
     4621 tccttaaggt gagcagcaac atccacaaca taccttttga attgaaattt cgcaaatttg
     4681 acagtacaac atcaacagat gagcatggtg atcttcaaga gtccgatgga ctgaaataaa
     4741 taaacacaat caattatgtt ccgttggtta gccttagtgg tgttcttcac gaccgcgttg
     4801 agcgttcaag cattgaatgt cagcattcta atttcacaat attattccat tgacaaaagt
     4861 ttagagccat atcttgaagt ctatttgcac acaattgctt ccaccgtgga ccccttacct
     4921 tccagctccg gtgatggctt aatggaaaaa aagatcaagg tactgatact ttttaaacag
     4981 aataacgaca tcgtaaatta cgataatttc atcattgaaa gtggaagaaa aaaggatatt
     5041 gaagatttcg taaccctcaa aaggtatggt atgaaaccgg gggaatacaa tattgaattt
     5101 acgcttgcag acctggcaaa cacaaacaat gaatacaggg atagtgtcac agtcaggata
     5161 aattcagccc ctgaacatcc tttcctgtct aatctccagt tagtggcaga cacaacttcc
     5221 agtgccgatg aacacattgc caagaacggc atcttttatg agctcattcc ttatgggtat
     5281 atgagccctg caatcaatcg attcggattg tattttgaaa gttacaacac agaccaactt
     5341 gccactgata tcgtaaacta ccatgtcaag ataagcaaaa gaatcaacaa tacctataag
     5401 cttgtcggcc gtactgcact taaaagacgt tctgcaagga ttgacgacat cctggcctgg
     5461 ttcgacgtaa cctattacga atccggccaa tacaatatca tggtatattt gtcggacaag
     5521 gacagaaatg caatagacag tgctgaatat tattttacga agagtaatcc tgtgagggac
     5581 gaagcaattc tttccaaaaa attcatggat gaagttgaaa cttccttcgc aggaaaatta
     5641 tccagggatc aattgaatta cactttgcgc gccattgcga tgaatgtaat caattcagat
     5701 gtggaactgg tcaaccgatt gatcaaggac gataatgtaa tcgccaaaag caactatatc
     5761 tataagtatt tcaaggaaaa gtcagctctt catcctgaag aatattatac ccaatatatg
     5821 gaagtcgcca aagcagtgga taagaaatat aaaacaggat ttggctatgg ctttgaaagc
     5881 gatagagggc ttatatttat gaaatacgga aagcccagcg acatgatatc catcacggac
     5941 gatccttcgt gtgccccgta tgaaatctgg caatacaatg aagtgcccaa actgatgcag
     6001 tccaatgtca aattcctatt cttcaacccc tttttggacg gaatggatta caggctttta
     6061 cagtccaatg caagaggtga gatgagaaat cccaactgga aaaaggagct gtacaaaacc
     6121 gtggcaacca atcccgaaaa ccaggcatct gaacgatatg aagtgccaaa aggattcaat
     6181 cgtcaggcag aagaatggct tcaggacctt tgattgcaaa acttatctta cctttgtgcc
     6241 tcaaatgctg aaaatgaaat ttaaggtttt ttccacgctt tgtgtcatcc tgttgttatt
     6301 atcctgtaag cgcgaaccta agccgatttc cgttgatatt cctgtttcag atgtaccaaa
     6361 aatcgattac agagtaaccg gaatcatacc acacaacaca tcatttttta ccgaaggctt
     6421 gctgatttat aatggtaaat ttctcgaaag cacaggcagc ccggaagaat tggaaaatgt
     6481 cgaatctatt gtggcgaccg ttgatatttc agatggaaag acagaagaaa aaataaggct
     6541 agataaaacc aggtatttcg gtgaaggtat cgtcgtactc catgataaat tgtaccagat
     6601 cacgtataaa aaccagaaag gatttatcta cgatgcaaaa acatttaaaa aactgggaga
     6661 attcggctat ccgagcaaag aaggatgggg aatgaccacc gacggcactc atgtcatcat
     6721 gagcgatggg acggatatgc tttccttcct gcttccacct gaaatgaaag tcgttaaaac
     6781 catccgcgtg acccaaaatg acataagtgt acgcaatgtc aacgaactgg aatggattaa
     6841 aggctacata t
//
LOCUS       DAOQQN010000157         6806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4687_length_6806_cov_6.390905, whole genome shotgun sequence.
ACCESSION   DAOQQN010000157 DAOQQN010000000
VERSION     DAOQQN010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4687_length_6806_cov_6.390905"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PLG24_07060"
     CDS             <1..561
                     /locus_tag="PLG24_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HMZ24685.1"
                     /translation="KSTTAIVGPNDDLILPMDSVKTDWEVELAVVIGRQASYVKQSEA
                     EEYIAGYMLHNDYSEREFQLECGGQWVKGKSCDTFAPLGPFMATRDEIPDPDNLRLWL
                     TVNDKLVQDGNTSDMIFGIPYLISYISRFMTLLPGDVITTGTPAGVGLGQKPEPWYLK
                     EGDTVALGIDGLGSSRQHVKPFKKKK"
     gene            558..899
                     /locus_tag="PLG24_07065"
     CDS             558..899
                     /locus_tag="PLG24_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HMZ24686.1"
                     /translation="MRRYALALDLVDDERLIAEYETYHKDVWPEILESIRSAGIVNME
                     IYRAGNRLFMVMEVNDDFSFEAKAKSDSTNPHVQRWEELMWKYQQALPTASPGEKWIL
                     MDKIFSLSHGG"
     gene            892..1713
                     /locus_tag="PLG24_07070"
     CDS             892..1713
                     /locus_tag="PLG24_07070"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMZ24687.1"
                     /translation="MDSHVHFWNYHPVKDAWITDEMQVIRRDFTPEMLSTILRDNGIS
                     GCVAVQSDQSETETDFLVAYAERFPFIMGVVGWIDLRAGHLEERLGHYSQQHIIKGWR
                     HIIQAEPAGFLLGDEFKKGIKLVGDHGYTYDILIHQGQLREAVEFAACFPNQKFVLDH
                     LAKPDIRQGRDKEEWRNNLRTMASLGNVYCKLSGMVTEADWHGWQPDQLTEYIDTVFE
                     CFGSDKIMYGSDWPVCILAADYSQVKNIVINYISRLSPEEQKAVMGVNATTFYNL"
     gene            1723..2511
                     /locus_tag="PLG24_07075"
     CDS             1723..2511
                     /locus_tag="PLG24_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ24688.1"
                     /translation="MDLQLRDKVMLITGGAMGIGGAICKALVREGAIPVIIDKNAEAG
                     ERLKSEIEDADGSCLFIPGELADPEACRKAVATAAAQFGHIDGLVNNAGVNDGVGLEH
                     GDYHRFIQSLHNNLIHYYMMAHYCLPYLKSTCGSIVNITSKTSVTGQGGTSAYAAANG
                     ARNALTREWAVELLKYNIRVNAIVVSECWTPQYERWIQTLPDAAATLAMIHSRIPLQN
                     RMTTPEEIADTAIFLLSGRSGHTTGQLVYVDGGYVHLDRALIKK"
     gene            complement(2528..4024)
                     /locus_tag="PLG24_07080"
     CDS             complement(2528..4024)
                     /locus_tag="PLG24_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24689.1"
                     /translation="MKPHLIRYYILILLLVFSTASVWSETPVIRFSPEAEHAYKLIFQ
                     LRLREAGRAIDEIKHSDPDNYVVYFLENYIDFFTLFIGENAADLKRLRNNKDVRLNKI
                     NKTDISSPYYLYCRAEINLQWALARLKFEEYTRAFFEVRSAFQDLQTNIRRFPSFAAN
                     KKSMGVLHAAIGTIPDEYRWGVSWIGGMDGTIDQGVRELEEVINYGRRNKFLFKDEAV
                     VMYAFVMLHLNNDPDRAWKIISESGIDPGDSPLGCFAMSNIAIRTGRNDLAISYLTNR
                     PSGPRYLPFYYLDFMLGSAKLHRLDRDANIYLEVYTKNFKGRNYIKEAYQKLAWYDLV
                     HGNTGGYRNKMIKVLKYGQDQLDEDKTALREAKTKVVPNALLLRARLLYDGGYLTRAA
                     KILNSANEAQFVNDNERLEFNYRRGRLAQSMGNDEDALRFYSITISKGRNLPAYFACN
                     AALNTGIIYEGQRRFDLARKNYNLCLSMSPSEYKASLHQKAKAGLNRL"
     gene            complement(4123..4881)
                     /locus_tag="PLG24_07085"
     CDS             complement(4123..4881)
                     /locus_tag="PLG24_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HMZ24690.1"
                     /translation="MAKKELSVTEKLRLLYDLQQIDSQIDEIRILKGELPMEVSDLED
                     EVAGLDTRVHKIETSIQTMENEIKSQQIHIKDCEAMISKYEKQLDKVKNNREFEALNK
                     EIEMQKLEIQLCEKKIREFKSSREGKNEALAAATERKAAKEEALQKKLVELKEIISRT
                     EKDEEKLIAVSEKQQKKIEDRLLKAYLKVRNTYRNGLAVVTVERNACGGCYNAIPPQL
                     QMEIALTKKILVCEHCGRILVDKASIMAEEPAEA"
     gene            complement(4887..5684)
                     /locus_tag="PLG24_07090"
     CDS             complement(4887..5684)
                     /locus_tag="PLG24_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HMZ24691.1"
                     /translation="MKLAELTQYLEAHAPLEYQESYDNSGLITGNPEMEITGATISLD
                     ATEAVVDEAIEAGYNLVIAHHPIVFSGLKRFTGQNYVQRTIIKAIRNDIAIYAIHTNL
                     DNVIQDGVNNKIAEKIGLADCRALLPYPGDLINDRPRGTGLIGMLTAPQEWSFFLENL
                     KQKMQVGVVRYTRPVSMTVSKVAVCGGSGSFLLSRAIAERADVFITADFKYHEFFDAN
                     DRIMIADIGHYESEQFTIELIKQLIVKKFSNFALRSTKIITNPINYL"
     gene            complement(5694..6416)
                     /gene="bshB1"
                     /locus_tag="PLG24_07095"
     CDS             complement(5694..6416)
                     /gene="bshB1"
                     /locus_tag="PLG24_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HMZ24692.1"
                     /translation="MNKIDILAIGAHPDDIELCASGTLLRMISLGYSVGLLDLTMGEL
                     GSNGTGPLRLQEAEDARQLMGAQWRHNLCLKDGFFDVNEVSIMAVAKIIRLTRPSIIL
                     ANAPYDRHPDHGRGAELVDRACFISGLAKVEIKDDGGSVLERWRPKIVLNFIQDRHIP
                     PDIVVDIKDYYRHKMEVISAYSSQFFMNKDRIATPISGEDFWHFLEARSREMGRAGGV
                     EMAEGFVCKTVPVVDDLFHLIH"
     gene            complement(6423..>6806)
                     /locus_tag="PLG24_07100"
     CDS             complement(6423..>6806)
                     /locus_tag="PLG24_07100"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HMZ24693.1"
                     /translation="AWHDAAARGDSAAFFGRMTKEAIYLGTDESERWDRTSMARDLGR
                     YFNGKKAWKFVSYNRHYIALKDRNSILFDECLKTWMGPCKATGLLSREKGSWKIAYYN
                     LSVAVPNDYVKQYTKLLPPGDILTD"
BASE COUNT         1766 a         1628 c         1542 g         1870 t
ORIGIN      
        1 aaatccacca cagcgattgt cgggccgaat gatgacctga tccttcctat ggacagtgtc
       61 aaaacagatt gggaggttga gttggctgtc gtcatcggaa gacaggcttc ttatgtgaag
      121 caatcggaag cggaagaata tattgccgga tacatgttgc acaatgatta cagtgaacgg
      181 gaatttcagt tggaatgtgg tggacagtgg gttaagggaa aaagctgcga cacctttgcg
      241 ccactgggcc cattcatggc gacccgagat gaaatacccg accctgacaa tctgcgtctt
      301 tggcttacgg taaatgataa attggttcag gatggcaaca cctccgatat gattttcggc
      361 ataccatacc tgataagtta tataagccgc ttcatgacct tgctgccggg cgatgtcatc
      421 actaccggca caccggccgg agtcgggctt ggtcaaaaac ccgaaccatg gtatctgaaa
      481 gaaggtgaca ccgtggcatt gggtattgac ggattgggaa gcagccgaca acatgtcaaa
      541 ccatttaaga aaaaaaaatg agacgatatg cccttgcatt ggacctggtg gacgatgagc
      601 gactgatcgc tgaatatgaa acctaccaca aggatgtttg gccggaaatt ctagagagta
      661 tccgcagtgc aggcattgta aatatggaga tataccgagc cggaaatcgc cttttcatgg
      721 tgatggaggt caatgatgat ttctcctttg aggcaaaggc aaaaagcgat tctaccaatc
      781 ctcatgttca acgatgggag gaattgatgt ggaaatatca gcaggcactt cccacagctt
      841 ctcccggtga aaaatggata ttgatggaca agatattctc cttaagccat ggtggatagt
      901 cacgtacatt tttggaatta tcatccggta aaggatgcat ggataacgga cgagatgcag
      961 gtcataagaa gggatttcac tccggaaatg ctcagtacaa tattgcggga caatggtata
     1021 tccggctgcg tcgcagtcca atcggatcaa agcgagacag agaccgattt tctggtagcg
     1081 tatgcagaaa ggttcccatt cattatgggt gtagtaggct ggatagatct gagggcaggc
     1141 cacctggagg aaagattggg ccattacagc caacagcaca tcattaaggg ctggagacac
     1201 atcatacagg ccgagccggc cggcttcctt cttggtgatg aattcaaaaa gggaattaag
     1261 ctggtaggcg atcatggcta cacttatgat attttaatcc accagggtca actcagggaa
     1321 gctgttgaat ttgcggcctg ttttcccaat cagaaatttg tactcgacca ccttgccaag
     1381 ccggatatca gacaaggccg tgacaaggaa gaatggcgta acaacctccg tacaatggca
     1441 tcactcggca atgtgtactg caaactctcc ggtatggtaa cagaggcgga ctggcatggc
     1501 tggcaacctg accagctgac ggaatatatc gacactgtgt ttgaatgttt tggaagtgat
     1561 aagatcatgt acggatcgga ttggccggta tgtatactgg ctgccgatta ttcacaggtt
     1621 aaaaatatag tcattaatta tatcagtcgc ttaagtccgg aagagcaaaa ggctgtgatg
     1681 ggcgttaacg ctacgacatt ttacaatcta taaatcgaaa caatggattt acaattacgt
     1741 gataaagtga tgctcattac cggaggagca atgggcattg gcggtgccat ttgtaaggca
     1801 ttggtcaggg aaggggcaat acctgtaatc atcgataaga atgccgaggc gggcgagcga
     1861 ttgaaaagcg agatcgagga tgccgatggc agctgtctct tcatccccgg cgaactggcc
     1921 gatcctgagg cctgtagaaa agcagttgca accgccgccg ctcaattcgg acacatcgat
     1981 ggtttggtca acaacgccgg tgtcaatgac ggtgtagggc tggaacatgg cgactaccac
     2041 cgctttatac aatccctgca caacaatctg atccattatt acatgatggc acattactgt
     2101 ttgccgtatt tgaagtctac ctgtggcagt atcgtcaaca tcacttccaa aacctcagtc
     2161 accgggcagg gagggacatc ggcatatgcc gcagcgaatg gagctcggaa tgctctcaca
     2221 agggaatggg ctgtggagtt gctcaaatac aatatacgtg tcaatgccat tgtggtctcg
     2281 gagtgctgga caccccagta tgagcgctgg atacagacct tacccgatgc cgcagcaacg
     2341 ctggctatga tccattcaag aattccgtta cagaacagga tgactactcc ggaagaaata
     2401 gcggacacgg cgatattcct gctttccggt cgctcaggtc acacaacggg ccagttggtg
     2461 tatgttgatg gtggatacgt ccatctcgac agggccctga tcaaaaaata gatggccaca
     2521 cctcattcta taatctattc agtccggcct tggctttctg atgcagactg gccttgtatt
     2581 ccgaaggaga catggataag caaaggttgt aatttttcct ggccagatca aaccttcttt
     2641 gtccttcgta gatgatgccc gtgttcaagg cagcattaca ggcaaaatag gcaggcagat
     2701 ttcttccctt actgatagta atggaataga atctgagggc atcctcatca ttccccattg
     2761 attgtgcgag gcggcctctg cggtaattga actccagccg ctcattgtca tttacaaact
     2821 gagcctcatt agcggaattg aggatcttgg ctgctcgggt caggtagcct ccgtcgtaaa
     2881 gcagacgcgc ccgcaacaac agtgcattcg gtaccacctt agtcttagcc tcccgtaagg
     2941 cggttttgtc ttcatccaat tgatcctgcc catatttcag caccttgatc attttgttcc
     3001 gataaccacc tgtattgccg tggaccagat cataccaggc gagtttttga tatgcctcct
     3061 tgatataatt cctgcctttg aagtttttgg tatagacttc aagataaata tttgcatccc
     3121 ggtccaggcg gtgcaattta gccgagccga gcatgaagtc aaggtaatag aacggtaagt
     3181 accttgggcc ggaaggccta ttggtcagat agcttatagc gaggtcattg cgcccggtgc
     3241 gtatggctat gttactcatg gcaaaacaac caagggggct gtcccccggg tcaattcccg
     3301 attccgaaat gattttccag gcacgatccg gatcattatt gagatgaagc atgacaaaag
     3361 catacatcac tacagcttca tccttaaaga gaaatttgtt tctcctgcca taattgatta
     3421 cttcttccag ttccctgact ccctgatcta tggtgccatc cattccccct atccaggaaa
     3481 caccccagcg atattcatcc ggaatggtgc caatagcagc atgaagtacg cccatgcttt
     3541 tcttgttggc tgcaaacgac gggaaacggc gtatattagt ctggagatcc tgaaaagccg
     3601 accttacctc aaaaaacgcc ctggtatatt cttcgaactt cagtctggcg agagcccact
     3661 ggagattgat ttcggccctg caataaagat aataagggga agaaatatcc gtcttgttga
     3721 ttttattcag ccggacatcc ttgttgtttc tgagtctctt caggtcagct gcattctcgc
     3781 caatgaatag ggtgaagaaa tcgatatagt tttcaaggaa atacactaca taattatccg
     3841 gatctgaatg ttttatttca tcgatggctc gtcccgcttc cctcagtcta agctgaaata
     3901 taagtttgta ggcatgttct gcttccggac taaaccgtat gacaggtgtc tccgaccata
     3961 ctgatgcggt ggagaatacc aaaaggagta tgaggatata atatcggatc aagtgtggtt
     4021 tcactgttag ctccgccttc cggctttata aaaaagaaaa agcctcaaac actacgccaa
     4081 aattagcatt tgaggcattc atattcaata accgggaatg attcaagctt cagcaggctc
     4141 ttccgccata atagaggctt tgtcaaccag tattcgtccg caatgttcac ataccagaat
     4201 cttcttggta agtgctatct ccatctgcaa ttgtggtgga atggcattat aacaaccacc
     4261 acatgcatta cgctccaccg tcactaccgc taatccattc ctgtaggtat ttcttacttt
     4321 cagatatgct tttaacagac gatcttctat ctttttctgt tgtttttcag agacagcaat
     4381 gagtttttcc tcatctttct cagttcggct gatgatttct tttaattcaa ccagcttctt
     4441 ctgtaaagct tcctccttgg ccgccttcct ttccgttgca gcagcaaggg cctcattctt
     4501 tccttccctg gacgacttga actcgcggat tttcttctcg cataactgga tttccagttt
     4561 ttgcatttcg atttccttgt tcaaagcttc aaattcccgg ttgttcttaa ccttatcgag
     4621 ttgcttctca tatttggaga tcatcgcttc acaatccttg atgtgtattt gctggctctt
     4681 gatttcattc tccattgtct ggatagaagt ttctatctta tgaacccggg tatctaatcc
     4741 tgcaacctca tcttcaaggt cgctgacttc cattggcaac tctcctttta aaattcgaat
     4801 ttcatcaatt tgagagtcga tttgctgaag atcgtacaac aatctaagct tttcagttac
     4861 tgataactct tttttggcca taatatttat aaataattaa tcgggttagt tataattttt
     4921 gtcgaacgaa gcgcaaaatt actaaatttt ttgacaatca gttgcttaat taactcaatt
     4981 gtaaattgtt cactttcata gtggccaata tcggcaatca taatcctgtc attggcatcg
     5041 aaaaattcgt ggtacttgaa atctgctgta ataaacacat ccgctctctc tgcgattgca
     5101 cgcgacagta agaaagagcc agaaccgcca cacacggcaa ctttggatac ggtcatactc
     5161 accggacgcg tatatctaac aacaccgacc tgcatctttt gtttcaggtt ctccagaaaa
     5221 aatgaccatt cttgtggcgc tgtcaacatc ccgatcaatc ccgtgccacg cggtctatca
     5281 ttgatgaggt cgccgggata gggcaacaga gcacggcaat cggccagtcc tatcttttct
     5341 gcaattttgt tgtttacacc atcttgtatg acattgtcga gattggtatg gatggcatag
     5401 atggcaatat cgttgcggat cgccttgatg atagtacgtt gcacataatt ctgcccggtg
     5461 aatctcttca gtcctgaaaa cacaatcgga tggtgagcga taacaaggtt gtagcccgct
     5521 tcgatagcct catccacgac ggcttccgta gcatcgaggc tgatggtggc accggttatc
     5581 tccatttcgg ggttgccggt gatcagtccg ctgttgtcgt aactttcctg gtactccagc
     5641 ggagcatgtg cctcgagata ttgagttaat tctgctaatt tcataccttt attttaatga
     5701 atgaggtgaa ataagtcgtc cactaccggc acagtcttac agacaaagcc ttctgccatc
     5761 tccacaccac ctgcccggcc catctctcta gacctggctt ccaggaaatg ccagaaatcc
     5821 tctccgctta tcggtgttgc tatcctgtcc ttattcatga agaactggga gctatatgcg
     5881 cttattacct ccattttgtg tcggtaataa tccttaatat ctactactat atctggtgga
     5941 atgtgcctgt cctgtataaa attcaaaaca atcttcggcc gccatcgctc cagaacacta
     6001 ccaccatcat cttttatttc cactttggcc agtccactta taaagcaagc cctgtcaacc
     6061 agctcggctc cgcgtccatg gtcgggatgc ctgtcgtatg gtgcatttgc cagaattatg
     6121 gacggccttg tcagcctgat aatttttgca acagccatga tcgacacttc gttgacatca
     6181 aaaaagccat ccttgagaca caggttgtgc ctccattgtg cacccatcaa ctgacgcgca
     6241 tcctccgctt cctgaagacg caatggccct gtgccgtttg agcctagttc gcccatcgtc
     6301 aggtcaagca ggcctacact gtaccccagg ctgatcatcc tgagcaatgt tcccgaagcg
     6361 catagttcta tatcgtccgg gtgagctccg attgcgagta tgtctatttt attcatattc
     6421 aatcagtccg ttaggatatc gcccggaggc aatagttttg tatattgctt tacataatca
     6481 ttgggaacgg caacactcag gttgtaatag gcaatcttcc agctgccttt ctcgcggctc
     6541 agcaatccgg tagccttaca aggtcccatc cacgtcttaa gacactcatc aaacagaata
     6601 gaatttctgt ccttcagggc gatgtaatga cgattgtagg atacgaattt ccaggctttc
     6661 ttcccattga aatacctacc cagatcgcgc gccatggatg tccgatccca ccgctcggat
     6721 tcatccgtgc cgagatatat tgcctcttta gtcatcctgc caaagaatgc agctgagtca
     6781 ccacgtgccg ccgcatcatg ccatgc
//
LOCUS       DAOQQN010000158         6804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4690_length_6804_cov_5.264039, whole genome shotgun sequence.
ACCESSION   DAOQQN010000158 DAOQQN010000000
VERSION     DAOQQN010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6804
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4690_length_6804_cov_5.264039"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2604
                     /locus_tag="PLG24_07105"
     CDS             <1..2604
                     /locus_tag="PLG24_07105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ24694.1"
                     /translation="IGTKTINYTYQDLFGCTWTETINITVNNCACANPATADAGGDMT
                     SCDGNGVTINGNSNYSVAWSTSGNGFFLPNDQDTTTYYPSSEDFTNGSVVLTMTTIDP
                     DGAGPCLPASSSMTLTFNIINIQISLITQVCETSDNIQLFALPAGGTWTGNGVIMNEF
                     VPSVAGQGIHALTYTVTSNGCKATKTVDITVSPAPTVTLAPFSDLCLNAPAIVLSGGS
                     PTGGQYYINGDFNNPVTTFSPTMIGTFTITYVAGPLNCKGNTTATITVKDCDCVDPVS
                     VNVGSNQTICSNQTAQVLATLNNNIGGTWTTSGDGTFSVPTGYQSEYIPGTNDILSGV
                     VTLTYTTIDPDDAGPCSPASASLQVTIIQLPDIVISPIQELCLDDLPVTLQASPAGGL
                     WTGKGISGDQFDPAKAGPGNHTLTYTVGTQNCTATKAITISVKDCGCPLTIGVDAGKD
                     TIVCQPGILRLNGSLTGISTGSWTSSGTGSFNDADLITPEYTPSPEDINNGSVTLTFT
                     SEDPDGDGPCLSVSDDVVVMIEKAPDVLLLVTQPNCIKNTGSIKINVIAGQNLQYSID
                     NGVTFTTDQTIENIPPGQYQLIVKNPAVLCQATLDFSIFPFTVPQGVWSSISAACSSQ
                     PLNSFTLESTDELTLPFQAVVNGVSYPIINSLPYVFDGLQTGAYQVEITDKNGCKMSK
                     KFVFDPGSSVNVTLDPVIVVDKGASALIDLKISGSYNTITWTPALYLSCSDCSNPIVS
                     PLENTEYTVIVKDENGCQDEATVLVRIRKHVNVFIPNAISPNHDGINDEFTVYTDDEV
                     YQVRSMKIFNRWGGVVFSKENFAPNDPRLGWNGRFKEQELNPAVFVYVIELEMKDGKT
                     KLYKGEFSLIR"
     gene            2771..5584
                     /locus_tag="PLG24_07110"
     CDS             2771..5584
                     /locus_tag="PLG24_07110"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HMZ24695.1"
                     /translation="MLHLKTYTLLFVLLLSWNANPLNLHGQNRLAKELQRLKLLYPIT
                     ELQVYNQAIENQGYTPLNQNHETESLLFETKAESIKKIENNKPKIIRIEIPRPSDRNL
                     MKILLFEYSPFAQGASVFNQNKELMDLETGVFYRGIIEGEENSLVALTFHDGIMEGLI
                     SSADGQYDVGKILNGDDQVIYKSSSLKMEPAFNCYTDDLTLDPGNVMPHNTVTVNNNG
                     CILVYFEITNSIYKYWKSVKAVNDYVGNFFNVVSTLYQNEAIDIKISELMVWTTNDSY
                     PTNSSEDALKSFKAYRTQFNGNFAHLLTYTSNNIGGLAYVDVLCDKDSRLGISNINMS
                     YNNFPNYSWTTTVVTHELGHNIGSKHTHWCGWPGGAIDNCEDTEGDCAPGPPPTNGGT
                     IMSYCHLKSYGINFSKGFGPLPGNLIREKVSQAGCLGPCCVITDITAKIVDVKCNGEK
                     DGGISVNEPTSGTPPYSYVWSNGATSRNMSGLIAGDYTVTITDSKNCVGIATFTVKQP
                     TPITISLKETSVCKGEPKSIAPKVNGGTPGYIISWTGPVNSQPTSTPVIFSDSGKYTI
                     KVQDANNCIISKDFDITYKNKFEASIMDKDGKVLPPSIEFCSRREMASGFFRLNGTIG
                     QTNQYTYYTCSWKWGNGKEETDSTAVRIDFEEQSGDFILTLEAQDKAFCWSYDTISFL
                     IRESPNELFDEVACGVYKIHSDFMFSYPQLPGKVVDLKNGLYFITGSFESKDSISDNF
                     GRCRFNPISKFSSSITDGSLIEDNLVIDECGPFLLAKHNNNFCYTWYKIDETTGTFEL
                     VENMNKSWLKVSQEDITLHQYYAVGRDCNNTNCDSVLVSTRSNTGDVIIPCIENNSKR
                     IMIYPNPNSGQFYATMESFQEGLYNVKIMDILGRVHYNQNIQIARQNELLPFNLPDTQ
                     NGIYFINIKGNDLNSTVRFTINH"
     gene            complement(5598..5954)
                     /locus_tag="PLG24_07115"
     CDS             complement(5598..5954)
                     /locus_tag="PLG24_07115"
                     /inference="COORDINATES: protein motif:HMM:NF016087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE"
                     /protein_id="HMZ24696.1"
                     /translation="MVDVAIHDGHNSLNAVDWVGTYRGTLPCADCEGIETVITLNEDG
                     TYKKNDTYLTNKEGENKFEEAGKFVWDESGSIITLQSSSGESKYKVGENKLFALDMDG
                     KLVEGQLADKYILVKE"
     gene            complement(6210..>6804)
                     /locus_tag="PLG24_07120"
     CDS             complement(6210..>6804)
                     /locus_tag="PLG24_07120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HMZ24697.1"
                     /translation="NAEIGAISIPQRKGDPVVFKEDEEFKNVFFDKIPSLRPAFTPNG
                     TVTAANASTINDGASTLILASGDKVKSLGLKPLARIVSFGDAEHDPEWFTTAPVEAAP
                     IALRRAGLTVDQIDFFEVNEAFAVVAMTFAKSFNIPYEKLNVYGGGVSLGHPLGSSGS
                     RILVTLTSVLQQEGGKYGLAAICNGGGGASSIVIEAL"
BASE COUNT         2174 a         1371 c         1315 g         1944 t
ORIGIN      
        1 attggcacaa agaccatcaa ttatacgtac caggatttat ttggatgtac ctggacagaa
       61 actattaata taactgttaa taattgtgca tgtgcaaacc ctgcaacagc tgatgccggt
      121 ggagacatga cttcgtgtga tggcaatggg gtcacgatca atggaaattc caattattct
      181 gtagcatggt ccacatcggg taatggtttt ttcttaccta atgatcaaga cactacaaca
      241 tactatccta gctcagaaga ttttacaaac ggaagtgttg tacttacgat gaccaccatt
      301 gatcccgatg gagcagggcc ttgcctacct gcatccagca gtatgacttt gacattcaac
      361 attatcaata ttcagattag cttaatcacc caggtatgtg agacttccga taatatacaa
      421 ttatttgcac tgccggccgg aggaacatgg acaggtaatg gtgtcattat gaatgaattt
      481 gttccatctg ttgccggtca gggcatacac gcactgactt atactgttac ctccaatgga
      541 tgcaaggcga caaaaaccgt tgacattacc gtatctcctg ccccaacggt cacattggct
      601 ccattttcag acctgtgcct gaacgctcct gccatagttt tgtccggtgg aagcccgaca
      661 ggcggacaat attatatcaa tggagatttt aataatccgg tgacaacttt cagtccgaca
      721 atgatcggga cctttaccat tacttatgta gccggtccac tgaattgtaa aggaaacact
      781 acagcaacca taactgtaaa agattgcgac tgtgtcgatc cggtaagtgt aaatgttgga
      841 agtaaccaaa ctatatgttc caatcaaact gcacaagttt tggcaacatt gaataacaac
      901 atcggtggaa catggacaac atccggagat ggcacctttt ctgtaccaac cggatatcaa
      961 tctgaataca ttcccggaac aaatgatata ttatccggag ttgttacact gacatacaca
     1021 acaattgatc cggacgatgc aggtccttgt agtcccgcct ctgcttcatt gcaggtcacc
     1081 atcattcagc tacctgatat tgttatcagt ccgatacaag aattatgcct ggacgatctt
     1141 ccggtaactc ttcaggcatc acctgccggt ggcttatgga caggcaaagg aatatcagga
     1201 gaccagttcg atcctgcaaa agccggtccg gggaaccata cgcttaccta tacagtggga
     1261 actcaaaact gcacagcaac aaaggcaata accatatcgg tgaaggattg cggttgccca
     1321 ttaacaatcg gagttgatgc aggtaaagat accattgtgt gccaacccgg tattctgagg
     1381 ctcaatggta gtttgaccgg catctcaaca ggtagctgga ccagttcagg caccggttcg
     1441 ttcaacgatg ccgacctgat cacaccggaa tataccccat ctccggaaga tattaataat
     1501 ggcagtgtaa ccctgacttt tacatcagag gatcccgatg gtgacggacc ttgcctttct
     1561 gttagcgacg atgtcgttgt aatgatcgag aaagcccctg acgttctttt actcgtcact
     1621 caaccaaatt gcataaaaaa tacaggaagc attaaaataa atgtaattgc gggacagaat
     1681 cttcaatact cgattgataa tggtgtaaca tttacaaccg atcagaccat tgaaaatatc
     1741 cctcccggcc aatatcaatt gattgtcaaa aaccctgcag tactttgtca ggcaaccctt
     1801 gattttagca ttttcccatt tactgtacca cagggagtct ggtcatccat ctccgctgcc
     1861 tgcagctcac aacctctgaa tagttttaca ctggaatcta ctgatgagtt gaccttacca
     1921 tttcaagccg tagtcaatgg ggtttcatac ccaatcatca attcacttcc ctatgttttt
     1981 gatgggttac aaaccggggc atatcaagtg gaaataaccg ataagaatgg ctgcaaaatg
     2041 tctaagaaat ttgtatttga tcccggatct tcggtaaatg tgaccttgga tcccgtaatc
     2101 gttgtggata aaggagcaag tgccttaata gaccttaaaa tatcaggcag ttacaacaca
     2161 attacctgga cacctgcttt ataccttagt tgcagtgatt gttccaatcc aattgtatct
     2221 ccattggaaa ataccgaata tacagttata gtaaaagacg aaaatggatg tcaggatgag
     2281 gcaactgtat tggtacgaat aagaaaacat gttaatgtat tcattccaaa tgcgatttca
     2341 ccaaaccatg acgggatcaa cgatgagttt actgtatata ctgatgatga agtgtatcag
     2401 gtgcgttcca tgaaaatatt caaccgctgg ggaggcgtgg ttttttcaaa agaaaatttt
     2461 gcacctaatg acccgagact tggttggaat ggccgtttca aggaacaaga attgaatccg
     2521 gctgtctttg tgtacgtgat agaattagaa atgaaggacg gcaaaactaa attgtacaaa
     2581 ggagaatttt cattgattcg ttaaaagagg tgcagaattc attttagata gcaaagacca
     2641 tcaaatagaa gctatttgct acaaatgtaa aattaattat atttacatcg gttgaggact
     2701 tatcgggtct ttttgatttc cgttagatcc tatacttctt gtaatggcca tttctttaat
     2761 tctttgatct atgttgcacc ttaaaacata tacgttactt ttcgtacttc tgctgagttg
     2821 gaatgctaat cctttgaatc ttcatggaca aaacagactt gctaaagagc tgcaaaggct
     2881 taagttgctg tacccgatta ctgaactgca ggtatataat caggcgattg aaaatcaagg
     2941 ttatactcca ttaaaccaaa accatgaaac agagagttta ttatttgaaa ccaaagcaga
     3001 atcaatcaaa aaaatagaaa ataataagcc gaaaattata cgcattgaga tccccagacc
     3061 ttcggaccgc aatctgatga agattttatt atttgaatat agtccatttg cacaaggcgc
     3121 ttcagtattc aatcagaata aagagttaat ggatcttgaa acaggagtat tctatcgggg
     3181 aatcatagaa ggtgaagaaa atagccttgt tgccttaact ttccacgatg gtatcatgga
     3241 aggattaata tcctctgctg atggacaata tgatgttggc aaaattttga acggagatga
     3301 tcaggtaatc tataagagtt catcattgaa aatggagcct gcatttaatt gctatacaga
     3361 tgatctcact ttagatcctg gcaatgttat gccgcacaat acagttactg taaataataa
     3421 cggatgtatt ttagtgtact ttgaaatcac caattcaatt tacaaatatt ggaagagtgt
     3481 taaggcagta aatgattacg ttggaaattt tttcaatgtt gtttcaactc tatatcagaa
     3541 tgaagcaatt gatatcaaaa tatcagagtt aatggtatgg actacaaatg actcatatcc
     3601 aacaaacagt tctgaagacg ctttgaagtc tttcaaggcc tatagaacac aatttaatgg
     3661 caactttgct catttactga cctatacttc caataatatt ggaggtctag cctatgtaga
     3721 tgttttatgc gataaagatt ccagattggg tatttccaat ataaatatga gttataataa
     3781 tttcccgaat tattcctgga caacaactgt tgttactcat gaactcggtc ataatattgg
     3841 ctcaaaacat acccactggt gtggatggcc gggtggtgcc atagacaatt gtgaagatac
     3901 tgaaggtgat tgcgctccgg gtcctcctcc aactaatgga ggaactatca tgagttattg
     3961 tcatttaaaa tcatatggga tcaatttctc aaaaggattt ggaccgctac ccggcaacct
     4021 tatccgcgaa aaagtatcac aggcaggctg tcttggacct tgttgtgtca ttacagatat
     4081 taccgctaaa atcgttgatg taaaatgtaa tggcgaaaaa gatggaggta tcagcgtcaa
     4141 tgaacctaca tccgggactc caccgtactc ttatgtatgg tccaatggtg caacaagcag
     4201 aaatatgagt ggattaattg caggtgacta tacagtaacc atcaccgatt ctaaaaattg
     4261 tgtaggtata gcaactttta ctgtaaaaca acctactccc attactattt cattaaaaga
     4321 aacatcagtt tgcaaaggtg aaccaaaatc aattgcacct aaagtcaatg gtggaacccc
     4381 cggatacatc atttcttgga caggacccgt caattcacaa ccaacatcaa cacctgtcat
     4441 tttcagtgat agcggaaaat ataccattaa agtccaggat gcaaataact gtataatcag
     4501 taaagatttt gatataacct ataaaaataa atttgaagcc agtataatgg ataaggacgg
     4561 caaggtattg ccgccaagta tagaattttg cagtcgcaga gaaatggcgt caggattttt
     4621 tcggttgaat gggaccattg gtcaaacaaa tcaatatact tattatactt gttcatggaa
     4681 atggggtaat ggtaaagaag aaacagactc aacagctgtc agaatagact ttgaagagca
     4741 atctggtgat tttatcctta cattggaagc acaggataaa gcattctgct ggagctatga
     4801 tacaataagc ttcctaatca gagagtcacc taatgaatta tttgatgaag tagcatgcgg
     4861 agtatacaag atacattccg attttatgtt ttcctatccc caattacccg gaaaagttgt
     4921 ggacttgaaa aatggacttt atttcataac cggttcattc gaatccaagg actctattag
     4981 tgacaatttt ggtcgttgcc gattcaatcc gatttccaaa ttttccagtt caataacaga
     5041 cggttcattg attgaggata atttggtaat agatgaatgc gggccatttc tcctggcaaa
     5101 acacaacaat aacttctgtt atacatggta caagatagat gagacgaccg gaacatttga
     5161 attagttgaa aatatgaata aatcctggtt aaaagtttct caggaagata ttacgcttca
     5221 tcaatattat gcggtcggaa gggattgtaa taacacaaat tgtgattcag ttctggtctc
     5281 tacaagatca aataccggag atgtaatcat cccgtgcatt gaaaataact ccaaaagaat
     5341 aatgatttat ccgaatccca attccgggca gttttatgca acgatggaaa gtttccagga
     5401 aggcttgtac aatgttaaga ttatggatat tctgggcaga gttcattaca accaaaatat
     5461 acaaattgcc aggcaaaatg agttgttacc attcaatttg cctgacaccc aaaatgggat
     5521 ttattttata aatatcaaag gaaatgactt aaactcaacc gttcggttca caatcaatca
     5581 ttgaaccggg ctctgcccta ttcctttact aaaatatatt tatcagccag ttggccttcc
     5641 accaatttgc catccatatc cagagcaaac aatttgtttt ctcctacctt atacttggat
     5701 tctccggaac tactctgtaa ggtgatgatt gagccggatt catcccagac aaatttaccg
     5761 gcctcttcaa acttgttttc gccttcctta ttagtcagat aagtatcatt tttcttatag
     5821 gtaccatcct cattgagggt tatcacagtt tcaatccctt cacaatcagc acatggcaaa
     5881 gtaccgcggt aagttccaac ccaatcaaca gcatttagag aattatgtcc atcatgaatt
     5941 gctacatcta ccatggtatc ctttgcagta ttattgttta aagatttcgt gctattactt
     6001 aggttacaag aaaataatgt acagcttata atagtcagca acaaaacttt taaccttgaa
     6061 ttcataaaga tcatattaat aatattttta atttgaaatg aacaaaaatt tgaatatcaa
     6121 cacattcaat gcacttaata tatcaaaacc aaaggaataa caaaggccat tcactaagca
     6181 gccggaaatt gaatctgcct taggatatgt tacaatgctt caataacaat ggaacttgcc
     6241 ccaccaccac cattgcaaat agcagccaga ccatacttac caccttcctg ttgcaggaca
     6301 gaagtgaggg taaccaaaat cctgctgcct gaagatccaa ggggatgacc taatgaaacg
     6361 ccgccgccat atacattcaa tttttcatat gggatgttaa aagatttggc aaaagtcata
     6421 gctacgaccg caaaagcttc attgacttca aagaaatcaa tctgatcgac agtcaaccct
     6481 gctcttctta acgcaattgg ggcagcttcc acaggcgcag tagtaaacca ttcgggatca
     6541 tgttctgcat caccaaaaga aacgatacgt gccaaaggct taaggccaag agattttacc
     6601 ttatcaccgg aagcaaggat caaggtggac gctccatcat tgatggtact ggcattggct
     6661 gctgtaactg ttccatttgg cgtaaatgcc ggcctcaggg atggaatttt atcgaaaaat
     6721 acattcttaa actcttcatc ttctttaaaa acaacggggt cacccttccg ttgaggtata
     6781 gagatggcac caatctctgc attg
//
LOCUS       DAOQQN010000159         6746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4754_length_6746_cov_4.632790, whole genome shotgun sequence.
ACCESSION   DAOQQN010000159 DAOQQN010000000
VERSION     DAOQQN010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4754_length_6746_cov_4.632790"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1573)
                     /locus_tag="PLG24_07125"
     CDS             complement(74..1573)
                     /locus_tag="PLG24_07125"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HMZ24698.1"
                     /translation="MRNLILITTLLGLYTHGVTAQVIDYVDDKYYKLEDGEVKPYKTI
                     KDPYSSDLASLMVTSAPEFEGDIQTSADGKQGKKDADGKIVIPFIYDALYMATEGLYN
                     ARIKRKWGLVNSAGKVLAPIEYDKPFSFDYELSMAKKNGQWGWIDMSGKTVIPIVYQE
                     AEGFVKKLARVKKGNLWGWVNKTGKEVIPPAYDDSKNFIDGLAFIKKNQKWGLINESD
                     KVVVKPQYDVVVYITYRLTAVGKDNYWGMMNGKGKLVSRIEYDAIGTCDIDDIAPIVK
                     KGKWGLINNHGKIVLPIEYDRLAAFKWNKTADVVKDGKMGRINKSGEVIVPPLYDSLI
                     DRGDNMIAVQLNKKWGLITSSGKEILPANYDEPLTVKSTYYIGKKNGSYGVVSQGQKA
                     VIPFIYDNLEFIDDNHFLVKKDDKFGIITKDNNPSVPIIYEKVLKPSNDKFLVKKEGK
                     WGCIDTENNIKIPFVYDEIRIGGMNTLKVKKEGKWFYIDFTGKCVKDCP"
     gene            complement(1928..5767)
                     /locus_tag="PLG24_07130"
     CDS             complement(1928..5767)
                     /locus_tag="PLG24_07130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ24699.1"
                     /translation="MKHFLLLNTKVLSYLIAALITIGGTGEMLADGSKDLFTPTTSGY
                     RQYLNVTNTLANYNPFPNNGVHKVYVNAGEVIYTGSSVVGLTLSGYGTGSVTIYSPTG
                     VQVAYSNTSTGRIDNRTQELNGPVRPGSTGSNYYTPLSYVATVSGIYEIQFSAFGPVS
                     GNNGTNIPDGPTGNTDLYRPLANASSWTQPVHANSHGLILAWDISVGTSTTNGTLIPG
                     RVYSTCLNLIGPNNRFEQSSFYGKVYVLTPEGFSYLVDNNGMNGASFNFFSNNKGIVS
                     TSGDFTSPPSYKSANTTTFVGELEDLIWDPRDADGSGNVTNKIFYVLPRLDLPASANV
                     HIGGTNTTTWLRQTPTVPTASNGFVDCDNNISFTSNVPGIYTIYIDADDNGNYEDPVD
                     VRLTGEVSSGINMVAWDGKDAAGNSIGANSVAILVRLTLAELHFPFLDVETNLNGIII
                     KRIDPETHLPLDDYDIVFWDDSNISHDNSQATAGASNPNVTGVDGVSSSSNGHKWGGG
                     PGSGDVNYYGNNKTMDTWTFTYAETELEVQFGDCITISGALWNDANASNTQNGGEPGI
                     SGTTSDGSGTITTGQALFVYLVDANGTVIGKSPVASDGTYLIEGVKRLADNLKLMIST
                     ADVPLGSVMTQPTLPDGWESVSENVHGSNTATQSQAGDGIINVTTGTTDISLQNFGIQ
                     QPPFAGNGEQESTNPGGTAQVSVDPSTFTNGDESSDNDGNVTSIRITDFPENATNIIV
                     DGVTYTLDGGNGTTPWPTTGVTVPTDASGNPTVDISVDPDFDGGGVIEIPFVSIDNAG
                     SESKVPGSAVIRFRSNISGNVFNDQNGLTDNTVNGVNITDALIGETLYAILVDDSGIV
                     VDKTTVNPSTGTYDFYGVPNGTYSIVLSTNDAVIGTTEPNPLLPEGWVYTGEHLGATT
                     GDDGLVDGSVHNIVVNNANVTDVNFGINRIPLADDKLYEVDSNVFTNGTPQSGFPVIP
                     GFQGIPMSSTNLVEYYNSSNGSLSGSDPEDCEDAGSCNGNTGGTGATFTIVGINENTK
                     LYYDFGGTTGVIEVTNNTTIPNFDVTRMVIYGEIGSGEAGDELGFTYTMTDKAGFVSS
                     SVADYIIRTNSPLPVQLLSFTAMKKQNTSELKWATASEEKNRGFEVLRSGDGRTWETI
                     GFVHSLATNGTSTQRLNYSMIDNRPAKGNNYYKLKQIDSNGRFGYSDIQRVYFSPEGS
                     IAVYPNPFQHKVNVAGLNGTETIVIYDFTGKEVNRINVKNINEEIMMDRLNPGPYNFV
                     FIASDGTTTTKRLVKVD"
     gene            6446..>6746
                     /locus_tag="PLG24_07135"
     CDS             6446..>6746
                     /locus_tag="PLG24_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24700.1"
                     /translation="MLRFLAHTVVFTLLCIFAGLPRYDLKAQGNSVITYTPEQGVCNY
                     NTSGMIRDHIGRIWVSSGGGVSRYDGREWKCFGIQDGLISIVIYNLKEDKFGNIWL"
BASE COUNT         1917 a         1447 c         1270 g         2112 t
ORIGIN      
        1 taagataatg tttaatatgt cttgaatcgt tacggcctat gaaatactat caccggtctc
       61 tggtgtcaac atcctaagga cagtccttga cacattttcc ggtgaagtca atgtaaaacc
      121 attttccctc ctttttaact ttaagggtat tcatgccacc gatacgtatc tcgtcatata
      181 caaaaggaat tttaatattg ttctctgtgt caatacaacc ccattttcct tcctttttta
      241 ccaaaaattt gtcattactc ggcttcaaaa ccttctcata aataatcggc acacttggat
      301 tattgtcttt agttattatc ccgaatttat cgtccttctt tactaagaaa tgattgtcat
      361 ctataaattc cagattatca tatataaatg gaatgacggc cttttgacct tgtgaaacca
      421 ctccatagga gccatttttt ttgccgatat aataagttga cttgaccgta agcggttcat
      481 cataatttgc aggcaagatt tccttaccgg atgaagtaat caagccccat tttttattta
      541 attggacagc tatcatatta tcccccctat ctataagtga atcatataat ggtggaacga
      601 tgacctcccc gcttttgttg attctaccca tcttgccatc ttttaccacg tcggctgttt
      661 tgttccattt gaatgccgct aacctgtcat actcgatggg aaggacaatt tttccatgat
      721 tattgatcag gccccacttc ccttttttta caattggagc tatgtcatct atatcacatg
      781 ttcctattgc atcatactcg atgcgagaga ccaactttcc ctttccattc atcattcccc
      841 agtaattatc ctttccgact gcggtcaatc tatatgtgat ataaactaca acatcatatt
      901 gaggcttgac cacaactttg tctgattcat tgatgagccc ccatttttga tttttcttta
      961 taaatgccag accatctata aaattcttcg agtcatcata tgcaggtgga atcacttcct
     1021 taccggtctt attaacccag ccccataaat ttcctttttt aacacgtgca agttttttga
     1081 caaatccctc tgcctcctga tacactatcg ggataactgt cttgccggac atatcaatcc
     1141 aaccccattg tccattcttt tttgccatcg acaactcata atcaaaagaa aatggcttgt
     1201 catattcaat cggtgccaaa acttttcctg cactattaac aaggccccat ttgcgtttta
     1261 ttctggcatt gtacagacct tctgtggcca tataaagcgc atcatagata aacggtatca
     1321 caatttttcc gtcagcatct tttttacctt gttttccgtc agctgatgtc tgaatatcgc
     1381 cttcaaattc cggagcggaa gtaaccatta gggatgctaa atctgaagaa tatggatctt
     1441 taatggtttt atagggcttt acttctccat cttctaattt gtagtactta tcatcaacat
     1501 agtcaataac ctgagctgta actccatgag tataaagtcc tagaagtgtc gtgatcaaaa
     1561 taagatttct catactaagt ttcttttgcc attttcttaa atatgaacca aaatacaaaa
     1621 aagaatttaa tgtagaaaag gattaaagta gctttattga ttaaatgtta agcaatggag
     1681 agtctttgta attaatcctc ccttgcatgt cattccggta aattgattat aaccctggtg
     1741 gtacttttaa aataaatgtc gaaaaaattg gtagttatta taacttagtc gtaagttgat
     1801 aggtattgtg agattacatg ctacaccttt ttaaatttct ttttgaaaac tatcctaaaa
     1861 agtaaaaaag acgccatctt ttaaaggatg gcgtctttta tttcatcgaa tggaagtaat
     1921 ggcccgatta gtctacttta acaagccttt tcgtcgtagt agttccatct gaggctatga
     1981 aaacaaaatt ataagggccg ggattaagtc ggtccatcat aatctcttca ttgatattct
     2041 taacgttaat tctgttcact tccttacccg taaaatcata gataacaata gtctctgtac
     2101 cattcaaccc cgccacattt actttgtgtt ggaatgggtt ggggtataca gcgatacttc
     2161 cctcaggcga aaagtagact ctttgaatat cactgtaacc gaaacgaccg ttgctatcta
     2221 tctgcttaag tttatagtag ttgttgccct tggcaggtct gttatcaatc atgctataat
     2281 taagcctttg tgtacttgta ccattggttg ccagagaatg gacaaaacca atggtttccc
     2341 atgtacggcc atccccgctg cgcagcacct caaagcctct gtttttctcc tctgaggctg
     2401 ttgcccattt caattctgat gtgttttgct ttttcatggc ggtaaaactt agcagctgaa
     2461 ccggaagggg cgaattggtc ctgatgatat agtcagcaac cgaagaactt acaaagcctg
     2521 ccttatcagt catcgtatag gtaaagccaa gctcatcacc ggcctcgcca cttcctatct
     2581 caccataaat aaccatcctg gttacgtcaa aatttggaat tgtggtatta ttagtcacct
     2641 ctataactcc ggtagttcct ccaaaatcgt aatacaattt tgtattttca ttgattccaa
     2701 caatagtaaa cgtagctccc gtacctccgg tatttccgtt acatgaaccc gcatcctcac
     2761 aatcttcagg gtcagaacct gaaagcgatc cattgctgct attataatat tctaccagat
     2821 tagttgaaga cattgggata ccctggaatc ccggaatcac aggaaaccct gactgaggtg
     2881 tgccattggt aaatacgtta ctatctacct catatagctt atcatcagca agcggaatcc
     2941 gattgatacc aaagttgaca tctgttacat ttgcattatt tacgacaatg ttgtggacag
     3001 aaccgtctac aaggccatca tctccggtgg tggctccaag atgttctcct gtatataccc
     3061 atccttcagg aagtaaagga ttcggttccg tagtgcctat tacagcatca ttggtagata
     3121 aaactatact ataggtacca tttggcactc catagaaatc ataggtgccg gttgaaggat
     3181 ttacggtagt tttatcaacc actatccctg agtcatcaac caggattgca tataaggttt
     3241 ctccaatcaa agcatcggta atgttgacac cattcacagt gttatcagtc aatccgtttt
     3301 gatcattaaa aacatttcct gatatattgg acctgaaccg gatcacggca cttccgggaa
     3361 ctttactttc agagcctgca ttgtcaatag atacgaatgg tatctctatc acaccgcctc
     3421 catcaaagtc agggtcaaca ctgatatcca cggtaggatt gccactagca tcggttggta
     3481 cagtcacgcc tgttgttggc catggtgtcg taccatttcc tccatccaag gtatatgtaa
     3541 ctccatcaac aataatatta gttgcatttt ccgggaagtc tgtgattcgg attgacgtta
     3601 catttccatc attatcgcta ctctcatcac cattggtaaa tgtagaagga tctacgctta
     3661 cctgagctgt accgcccgga ttggtggatt cctgttcacc attaccggca aacggtggct
     3721 gttgaatacc aaaattctga agcgagatgt cagttgttcc tgttgttaca ttgataatgc
     3781 catcaccagc ctgactttgt gttgctgtat tagaaccatg tacattttca ctgacacttt
     3841 cccagccatc aggaagagta ggctgagtca ttactgaacc aagaggaaca tcagctgtac
     3901 ttatcatcaa ttttaaatta tcggccagac gctttacacc ctcaatgaga taagtaccat
     3961 ctgaggctac cggcgatttc ccaattacgg taccattggc atctacgagg tatacaaaaa
     4021 gtgcttgacc ggtagttatg gtacctgagc catcacttgt tgtaccactt attccgggtt
     4081 cacctccgtt ttgtgtattg ctggcattgg catcattcca taatgcacca ctgatggtaa
     4141 tacagtctcc aaactgtacc tccaactctg tctctgcata cgtgaaggtc caggtatcca
     4201 ttgttttatt atttccgtaa taatttacat caccggatcc cggtcctccg ccccatttat
     4261 gaccattgct ggaggaagaa acaccatcaa ctcctgtaac atttggattg gaggctcctg
     4321 ctgttgcttg tgaattgtcg tgagaaatat tggaatcatc ccaaaaaacg atatcatagt
     4381 catcaagtgg aagatgagtc tctggatcta tccttttgat gatgattccg ttgagattgg
     4441 tttcaacatc caaaaacgga aaatgtaact cagccagggt caacctgacc aggatagcga
     4501 ccgaattggc tcctattgaa ttcccggctg catcctttcc atcccaggca accatattga
     4561 taccggaaga tacttcacct gtaagtcgga catcaaccgg atcttcgtaa tttccgttat
     4621 catcggcatc tatataaatg gtataaattc ccggcacatt cgatgtaaag gaaatattgt
     4681 tatcgcagtc tacaaaacca ttactggctg ttggcaccgt tggagtttgt cgcaaccagg
     4741 tagtcgtgtt tgtcccccca atatgcacat ttgcagaagc aggaagatcg agacgcggaa
     4801 ggacataaaa aatcttattg gttacattcc ctgatccatc tgcatccctt gggtcccata
     4861 tcaaatcttc tagttcgcca acaaaggtgg tagtgtttgc tgacttatag cttggagggc
     4921 ttgtgaaatc gccggaagta ctgacaattc ccttgttatt actaaagaaa ttgaacgaag
     4981 ctccgttcat tccattatta tcgaccaggt atgaaaaacc ttccggagtt aacacataaa
     5041 ctttaccata aaaactgctt tgttcgaatc tgttattcgg tccgatgaga ttcaggcagg
     5101 tagaatacac cctccccgga atcaaggtgc cattggttgt acttgtccca actgagatat
     5161 cccaggccag aatcagacca tgggagttag cgtgtacggg ctgagtccag ctggatgcat
     5221 tagccaaagg gcgatacaag tctgtgttgc ctgtcgggcc atcggggata tttgtcccgt
     5281 tattcccgct aacaggccca aaggctgaaa actgaatctc atatatacca cttacggtag
     5341 ccacataact gagaggagtg taataattcg aacctgtaga tccgggtctt accgggccgt
     5401 tcagttcttg cgtacgattg tctatccttc ctgtacttgt gttgctgtat gccacttgca
     5461 cacctgtggg agagtaaatg gttacacttc cggtaccata ccctgaaaga gtcagaccca
     5521 caacactact tccggtatag atcacctcac cggcattgac atagacttta tgtacgccat
     5581 tgtttggaaa agggttataa tttgccaggg tattggtaac attgaggtat tgtctgtacc
     5641 ctgacgttgt aggagtgaat agatctttac tcccatcagc taacatttca cctgttccac
     5701 caatggtaat caaagcggca atcaggtaag aaagcacttt tgtgtttaaa agtagaaaat
     5761 gtttcatgaa aatagtattt atatatcaat aataaaagag tttaacaaac ccattattac
     5821 gacctgattg tcaatgcagt atgataaaag gaataatgtc tattcagaga agtcgataga
     5881 catttatgca tcagataata aaaggtagta ataaagtagg cgcaaatata atttaataaa
     5941 ataaagtata gcgaaaattt gcagttgagc ccaaaattta gataaaaaaa attgtgttga
     6001 gcggaatata cttctaaaat tggttagtaa aaaaagccct atatgaaatt gtatggtctt
     6061 gtaaatttgt gctatgaatg gtaatgattt gcctcagatg gcgttgcaat cacgaaagcc
     6121 atggtatgtc aaagagacgc aatttgtaac aatttcggag aaggggaggg agttacatag
     6181 ttttttttta gtaccattaa tctgggtata ggtggatgaa aaatagactt tgaaatatca
     6241 gccaactcca tattaattgc agtagttact cttttctcaa taaaaaatta agaagtgtca
     6301 tattgacaaa atacagcctg acacttttct gtttcatggc ttaaaagtcg gcttttgcgt
     6361 ccggaaataa ccttttcgtt ctcataccgg acattatttc agccaaaatg gattatattg
     6421 tctcctgaat attttaaaga atctcgtgtt aagattcctt gctcatactg ttgtttttac
     6481 tctcctatgc atctttgcag gattgccccg ttatgatttg aaggctcagg ggaattcggt
     6541 aataacctat actcctgagc agggggtgtg taattataat acttccggta tgatcagaga
     6601 tcacataggc aggatctggg tctcgtcggg cggaggagtc tcgcgatatg atggccggga
     6661 atggaagtgc tttggcatac aggatggatt aatctctata gtaatatata acctgaaaga
     6721 agacaaattt gggaatatat ggttgg
//
LOCUS       DAOQQN010000160         6745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4755_length_6745_cov_6.170703, whole genome shotgun sequence.
ACCESSION   DAOQQN010000160 DAOQQN010000000
VERSION     DAOQQN010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4755_length_6745_cov_6.170703"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1597
                     /locus_tag="PLG24_07140"
     CDS             <1..1597
                     /locus_tag="PLG24_07140"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF025228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HMZ24701.1"
                     /translation="SVLLLIIAFSLGFSTSRAQTRSGQMPIIPDSVKVFQDNNYTGRG
                     NIDEPVLHIVIPVSEGSRPVLSEVHLNFKGTSHLKDITQCRIYNTGNVNAFHPGKSSG
                     DLLATARIRRSELTLKTRGGLLPGDNHLWLTVDVSPKAGEGNKIDVALESIRVDDRNY
                     PIVNGNPEGSREILLKRVLVLAPGDYGSKNYRIPAIITADDHSLVIVTDKRKDNSIDL
                     PEDIDIIAQRSTDGGKTWSEPVTVAKGEGRYKGYGDACITRTLSGKLVALYVGGPGLW
                     NSTSEKPQGHYVSVSDDNGKSWTGPIDITGQLYGINCPDSVRRHWQSSFFGSGRGLTL
                     RDGRIMAVIAVRESKENHKLNNYVVYSDDEGTTWHVSQKAIDGGDEAKVVELNDGTIL
                     LSSRTAGNRLWAKSTDRGVTWGPKNTWHEIWGNACDADIIRYTSTRDGYDKDRILHTL
                     PNAKNRTNVSMWLSYDEGTTWPVRKTICKGESAYSSITILPDGTIGVYVEEDESVPYK
                     MYFLNFSLDWLTDGKDHFRKAGG"
     gene            complement(1684..2211)
                     /locus_tag="PLG24_07145"
     CDS             complement(1684..2211)
                     /locus_tag="PLG24_07145"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ24702.1"
                     /translation="MDKYSGLLHPISTFIPSGVPQHWADLGCGTGVFTRILAQYLPES
                     SKITAIDKVLQHFPETMGNSINIDFRVGDFENESLPLQHLDGIMMANSLHYVRDKEAF
                     ILQAEKYFAKNPAFLIIEYNRTEANQWVPYPIPFEQLKTLFSRLDYTHIEKIGEQKSL
                     YGGVMYACYVRKEVD"
     gene            complement(2235..2672)
                     /locus_tag="PLG24_07150"
     CDS             complement(2235..2672)
                     /locus_tag="PLG24_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24703.1"
                     /translation="MKLYTLLLCSLLALTACGQTKEKDKNEKIVNTPKTQKIMDLNKI
                     SNTQVKQAIEALQNNDKTAWYSHFTDNAVFSDDGRKSDFKTFFDNAFSHKERFLDIDK
                     VENDGKDIYGNFYAGQWGTFRVYFKFHQQADGKFNRIDIGQAN"
     gene            complement(2675..3025)
                     /locus_tag="PLG24_07155"
     CDS             complement(2675..3025)
                     /locus_tag="PLG24_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HMZ24704.1"
                     /translation="MLIKIINAGLILFAVFMGFKQGWNMLTTKPEMLELFGKWNFDKN
                     FIIINGAVTILSAILILFPKTFVWGNFLMALGILLIICLQLQIKDLRGAAIEVPFLLL
                     NLIIIYLQHPLKNS"
     gene            complement(3271..4815)
                     /locus_tag="PLG24_07160"
     CDS             complement(3271..4815)
                     /locus_tag="PLG24_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24705.1"
                     /translation="MKQFFTCIISIIVLNIGLSQGTVFEFSGPDSYFSERLTVDGDDI
                     YFAFTSESQSIYSTYIARWNTLSEKIEWCRRLTVDNNVSINPVKILLRKDGTILLGAY
                     DYSWKDGFSNGNITFILFDKNGKTLQTKRLGSPTGGIMRNLLADDDDSVVFIADRTNA
                     QSEYKSLVGRLDKDLNVVNMRAVFKDYYTYGLGLDKDKEGNIYTVGHSRPANSGSSRS
                     IVTKWSKDLDHIFTIMHLDKDPNSSFNFIHIDDENQIHLGGNLNNLVKYVRLNKDLKY
                     EYGHEFTFGYPRNIWKDKNGHTNIFIDGPNYFVRLDNKNTAQQFISYGSVGNTSGQQY
                     IPGEERIYNFSYKNSATEPQNRLYISSHTYSADNKCILFDISHDNNKPLSLDSFGITN
                     VTITDESAKAIVPDDIIVSDFDLTVKEICRVEQASTEGERALIPLTLYPNPAFDPIYV
                     KHDDNIRITKGEFIGINGNIINIPHSEIQSKTISTAGLITGIYILRLYSSDGRQIINK
                     VNILKP"
     gene            5734..6027
                     /locus_tag="PLG24_07165"
     CDS             5734..6027
                     /locus_tag="PLG24_07165"
                     /inference="COORDINATES: protein motif:HMM:NF021431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HMZ24706.1"
                     /translation="MRVIAKKILREFWEKHSDCEQQLKSWYQEADKGTWKSLNELKSE
                     YPSASVLEDNRVCFNIKGNNYRLIVKINFNYQMMWIRFIGTHAEYDKIDANKI"
     gene            6033..6389
                     /locus_tag="PLG24_07170"
     CDS             6033..6389
                     /locus_tag="PLG24_07170"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ24707.1"
                     /translation="MKLKPIKTEHDYHQALERLEQIFDSKPGTKEGDELEILGILIEN
                     YENDFFPIDLPDPIEAIKFRMEQLNYSQNDLAEVIGLKSRASEILNRKRKLTLEMIRK
                     LAEKLHIPTEVLIQAY"
BASE COUNT         2118 a         1231 c         1394 g         2002 t
ORIGIN      
        1 gtctgtccta ctccttataa ttgcattcag ccttggattt tccacaagcc gggctcagac
       61 aaggtccggc cagatgccca ttattccgga ctctgtaaaa gtctttcagg ataataacta
      121 caccggaaga ggaaatatcg atgaacctgt cctgcacatt gttattcccg tcagtgaagg
      181 tagccggccg gttttgtcag aggtgcattt aaactttaaa ggcacaagtc atttgaagga
      241 tatcactcaa tgcaggattt ataatacagg caatgtaaat gccttccatc ccggcaagtc
      301 ttcaggtgat ctgctggcaa cagcccgaat acggcggtct gaactgactt tgaagacaag
      361 ggggggcctc cttccgggtg acaaccatct ctggctgaca gtggatgttt caccaaaagc
      421 aggagaagga aataaaattg acgttgcgct ggagtcaatc agggtggacg accggaatta
      481 tccgatagtc aacggcaatc ctgaggggag tcgtgagata ttgttaaagc gagtgctggt
      541 actggctccg ggggattatg gttcgaaaaa ttatcggatt ccggcaatca ttactgccga
      601 tgaccattct ctggttatcg tgaccgacaa gaggaaggac aatagtattg acttaccgga
      661 agatattgat attatcgccc aaaggagtac ggatggcgga aaaacatggt ccgaaccggt
      721 gactgtggcg aagggtgagg gcaggtataa aggttacggc gatgcctgta tcaccaggac
      781 attatccggg aagctggtgg cactgtatgt cggtggaccg ggattgtgga actctacctc
      841 agaaaagcca cagggccact acgtgagtgt cagcgacgac aatggtaagt cctggaccgg
      901 cccaatagac atcaccggtc agttgtacgg aatcaattgt ccggattctg tccgccgtca
      961 ctggcaatct tcgtttttcg gctccggcag gggactgacc ctccgggatg gccgcatcat
     1021 ggcagtgata gctgtgcggg agtcgaaaga aaaccacaaa ctcaacaatt acgtggtcta
     1081 ttcggatgat gaaggcacta cgtggcatgt ctctcagaaa gccatcgatg gtggtgatga
     1141 ggccaaggtc gtggagctga atgacggaac cattttattg agcagcagga ctgccggcaa
     1201 caggctttgg gctaaatcca ccgacagagg cgtcacctgg ggtccaaaga atacatggca
     1261 tgaaatatgg ggcaatgcct gtgatgcaga catcatcaga tatacatcca ccagggacgg
     1321 ttacgataaa gaccggatcc tgcacacctt gcccaacgcc aaaaaccgga ccaatgtgag
     1381 tatgtggcta agttatgatg aaggaacgac ctggccggtc aggaaaacca tctgtaaggg
     1441 cgagtcagcc tattcgtcca taaccattct tccggatgga accatcggcg tctatgtcga
     1501 agaagacgaa agtgtgcctt ataagatgta ttttctcaac tttagcctgg attggttgac
     1561 agatggaaag gatcatttca ggaaggcggg cgggtaaatg attgcgtgat gtggggttga
     1621 atggtttgct tgtggcacta tcttgcaatt gataacccgg attacatgga tgtggcatat
     1681 ttttcaatca acttccttcc ttacataaca tgcatacatc acgccaccat agagcgactt
     1741 ttgttcgccg attttttcaa tgtgcgtgta atcgaggcgg gaaaacaagg tcttcagttg
     1801 ttcaaacgga ataggataag ggacccattg attggcttcg gtgcgattgt attctatgat
     1861 cagaaaggcg ggatttttgg caaagtattt ttctgcttga aggatgaaag cttctttgtc
     1921 tctgacataa tgaagcgagt tggccatcat gattccgtca agatgttgca gtggtaatga
     1981 ctcattttca aaatcaccga ctctaaagtc aatatttata gaatttccca ttgtctccgg
     2041 gaaatgttgc agaaccttgt caattgccgt aattttactt gattccggta aatattgcgc
     2101 caatatccgg gtaaacacac cggtcccgca gcccagatca gcccaatgtt gaggtacacc
     2161 ggaagggatg aatgttgaga tcgggtggag gaggccggag tatttgtcca ttgatttaca
     2221 gaaagaaaat caggttaatt agcctgcccg atatctattc ggttaaattt gccatcggct
     2281 tgttgatgaa atttaaaata aaccctgaat gttccccact gacctgcata gaagtttcca
     2341 taaatatcct taccgtcatt ttctacctta tcaatgtcta aaaatcgctc tttgtgacta
     2401 aaggcattgt caaagaaggt tttaaaatct gattttcttc catcatcaga aaatacagca
     2461 ttatctgtaa agtgtgaata ccaggcagtc ttgtcattat tctgcaatgc ctcaattgcc
     2521 tgttttactt gtgtatttga gattttattc aggtccatga ttttttgtgt tttaggagta
     2581 tttacaatct tttcgttttt atctttttct ttagtctgac cacaggcggt taatgccagt
     2641 aaggaacata aaagtaatgt atacaatttc atattcaact gttttttaat ggatgttgta
     2701 aataaattat tatcagattg agcaataaaa acggcacttc tatggcagca cccctcagat
     2761 ccttgatttg taattgtaaa caaataatca gtaagattcc aagagccatc aggaaattac
     2821 cccaaacaaa ggtttttggg aaaagtataa gtatggctga aagtatggtt acggctccat
     2881 tgattattat aaaattttta tcaaaattcc atttgccaaa caactcaagc atttcgggct
     2941 ttgtggtcaa catgttccat ccttgcttga atcccataaa tacagcaaac aatatcagtc
     3001 ctgcattaat gatttttatc aacatcgtat ataatttatt ttaggaataa gtttaaagtg
     3061 ataaatatat ataaaataat ggccaaaata ttgttaaaat gtcagatttt gtattgattt
     3121 ttaattaaaa tacaaaaatg ttgttttatc acatgcctta aatagcagat attctaattt
     3181 ttttaatgga cagacataac tactggtttt gtaaaatctt ttatccaggt aattggcaaa
     3241 tacccgggat cttatttttg gtcgtagaat ctaaggtttt agaatattta ctttgtttat
     3301 aatctgcctg ccatccgagc tgtagagcct taatatatag attcctgtaa tcagccctgc
     3361 ggttgaaatt gttttacttt gaatctccga atgaggtatg ttgattatat tcccgttaat
     3421 accgatgaat tcgcctttgg ttattctgat attgtcatca tgttttacat aaataggatc
     3481 aaaagccgga ttcggataca gggtaagggg tatcaaagct ctctccccct ccgtagaagc
     3541 ctgttctacc ctgcatattt cctttactgt gagatcaaaa tctgagacaa tgatatcatc
     3601 cggtacgatg gcctttgccg attcatccgt tatggtaaca tttgtgattc caaaactatc
     3661 aaggctcaaa ggcttattgt tatcatggga aatgtcaaat aaaatacatt tattatctgc
     3721 actgtaagta tgtgaagaga tgtaaagcct gttttgcggt tccgttgctg aatttttata
     3781 tgaaaagtta tatatacgct cttcaccggg gatgtattgc tgtccggaag tattgcctac
     3841 ggatccatac gaaataaatt gctgagccgt attcttgtta tcgagtcgta caaaataatt
     3901 cggtccatca ataaatatat tggtatgacc atttttgtcc ttccagatat tgcgtggata
     3961 gccaaaggta aattcatgtc cgtattcata ttttaaatcc ttattcaaac gcacatattt
     4021 tacaaggttg ttcaaattac cgcccaggtg aatctgattt tcatcatcaa tatgtatgaa
     4081 attaaacgag gaattcgggt ctttgtccag atgcatgatg gtaaaaatat gatccagatc
     4141 tttactccat tttgtgacga tacttcttga agaaccagaa ttagcagggc gggaatgtcc
     4201 tactgtgtat atatttccct ctttatcttt atccagtcca agcccatatg tgtaataatc
     4261 cttaaagact gcgcgcatgt tgaccacatt gagatccttg tccaatctgc ctaccagtga
     4321 tttgtattca gactgggcat ttgtcctatc agctataaaa acaacagagt cgtcatcatc
     4381 ggccaacaga tttctcataa tgcctccggt gggacttccc agtcgttttg tttgaagtgt
     4441 ttttccattt ttatcaaaca gaataaatgt aatattcccg ttggaaaaac catctttcca
     4501 ggaatagtca tatgctccca ggaggatggt accatctttg cggagcagga tttttaccgg
     4561 attgatggat acattattgt caacagtaag ccttctacac cattctatct tttcggataa
     4621 tgtattccac ctggcgatat aggtagaata tatggactgg ctttctgaag taaaggcaaa
     4681 gtagatatca tctccgtcaa cggtcaaacg ctcgctgaaa taactatcag gtccgctaaa
     4741 ttcaaacacg gtaccttgag ataaaccaat attcaatacg ataattgaaa taatgcaggt
     4801 aaagaattgt ttcactgtat ttttaatttt aaaggatttt taattattca atatgccggt
     4861 agcgcctaag ttcttccgat gatattgagt atgatggttt ctggttatga cgtgcgaaat
     4921 caacgtggtt gtctctggca atagtgcaaa aaataaatgt aagaaatcaa agaacaggaa
     4981 tcggttttat cccgaaatca gggcttccgc aaatataaaa aaagatagca aaaaacagaa
     5041 tattctaatc ggttttacaa cttataaaca taaaaaataa gttcagtatg tgaataacag
     5101 tacttaagat cacttcatgt atccatggtg tgtatatctg attacccttt tagattcatg
     5161 aaatgaagag atttgcagcg cccggcaaat aataaaacag agttgtatgg gtcatgccaa
     5221 ttagataact tatttctgtt tccttgataa aaacgtaata cagaatgtgc cgtcgtaaaa
     5281 gtccaatttt attgaccgga gaaacacgga ttgttaaaat ggttaaaatg taagagtaca
     5341 ttaatcacaa aaatactatt ctgagacttc cgataaattc ttgtcccggc taagacaagg
     5401 cagattctta ttaaataaaa gacctgaaaa ttaaaatcga atgttgattt atcaggtgga
     5461 tattgatttt gcatagaatc gcataagtaa ccacactgtc cgacaacaga gaatagaatt
     5521 caaattagag ttgggctgat taagaattat aaactgtccg tagcgtagat tggatatatg
     5581 acaaaaagtt gtaactttat ttgcgaaata tacgccatat ggcgtatata catatgttgc
     5641 cagaaatcct atcaaaacac atagcacaaa attttcattg aacacatttc ccaaaatggg
     5701 aacctgttac tatctttgtc aaaagaatta aaattgagag ttatagctaa aaaaatatta
     5761 cgtgagtttt gggaaaaaca ttctgattgt gaacaacaat taaaatcgtg gtatcaagaa
     5821 gctgataaag gaacttggaa aagtctgaat gaattaaagt ctgaatatcc aagtgcaagt
     5881 gttttagaag ataatagagt ttgttttaac ataaagggaa ataattatcg tttaatcgtt
     5941 aaaataaatt ttaattatca aatgatgtgg attcgattta ttggaactca cgctgaatat
     6001 gataaaatag acgcaaataa aatttgacaa agatgaaatt aaaaccaata aaaacagaac
     6061 acgattatca tcaagcttta gaaagacttg agcaaatctt cgactcaaaa cctggaacaa
     6121 aagaaggtga cgaattggaa attttaggga ttttaattga gaattatgaa aatgattttt
     6181 ttccaattga ccttcctgat ccaatagaag ctattaaatt taggatggaa caactgaatt
     6241 attcacaaaa tgatttagcc gaagttattg gattgaaaag tagagctagt gaaattctaa
     6301 atagaaaacg aaaattaacg cttgaaatga ttagaaaact agctgaaaag ttacatattc
     6361 caacagaagt tttaattcaa gcatattaaa aatcaggact tctgctaaca gtgccaaaag
     6421 cagtgcagaa cggattcgac caggacccgg tccatggacg accatgacaa cattcacgag
     6481 tagcgtccat gatggtaaaa ttgccgctcc ttagctgatt tgccggatgt tggcggtcat
     6541 tgtagaacga cacctgaacg aaagatgaga taaaagtgga aatacaacat tcagacattt
     6601 caaaatttga aattaaagaa atagcatggc aaggaccttt ttcgtggaca ggctacgaaa
     6661 atcagaataa ccttgacacc attcctgaca cagaaggtat ttatctttgg acatttcaat
     6721 ataatgacgg atatttggtg tattg
//
LOCUS       DAOQQN010000161         6652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4868_length_6652_cov_4.464757, whole genome shotgun sequence.
ACCESSION   DAOQQN010000161 DAOQQN010000000
VERSION     DAOQQN010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4868_length_6652_cov_4.464757"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3581
                     /locus_tag="PLG24_07175"
     CDS             <1..3581
                     /locus_tag="PLG24_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HMZ24708.1"
                     /translation="DLTESARLLAAELKRCLSFGKPVQVIAHSMGGLVMKQLMIKEKS
                     TWHSFCQSSSNRLILLGTPWLGSHLIMEVLTGHSSKVKLLGLLDKIHDREQIMSTAAG
                     FPGVIQLLPMTDEGFGTSVFWENFRPYTNHLAVPPSHMFDLYNNYKQETAGFRMDEAH
                     LDNVFYIAGHDETTSSYKIEWKFLQGKRLVFRSTDAGDGSVTWKLGIPPELKRSNLYY
                     SEITHGELANKQIIIDTIMDIFLSGKSSRLPQTEPGSRGIQQEGIVPEFDFIPNNDDE
                     LVDALFQGKIGRREAKPQTEPLQVSVYHADLKYSSHPVLVGHFLNDGLYSAEKSLDSY
                     LGNKLSERLRLGYYPGNIGECEITYQSESRPKGALVAGLGLADELTSYRLSLTVESAI
                     IKYAFFFRDNYSRSENKRVASGISTVLVGSSFAGIPINECLRAILSGIQRANERMVQL
                     NIGLQLIRQVEFVDYYEDLAQQCYSILKDFERKSDDFNIEVAPFRKGPGMRRRLTLGD
                     TSSWWRTFNTVTNFDRLTGRPSGFTFSLTSGRARIEQGGVTTDLKLVEYLAKEYSHKN
                     DWNPRLSKTMFELLIPNDFKNIIRNQSNMVWKLDEYAAQFPWELFHDYNDITNAHREK
                     PAFVNTGMIRQLITEDFRRNPTVVTEDKALVIGDPDYSGTVFSQLQGAEEEARVIGKK
                     LVEDNFEVRSMIHSRAGDIITELFSDKYKIIHIAAHGVYGEVAVNDSKEFKAGVVLGN
                     DVYLEASMINQLSTIPEFIFINCCYSGDMTGLDEKYFKERSALAANIGTQLVRMGVKA
                     VIVAGWAVHDGAALTFSTELYDRMLSGFEFGEAVLSARRKCYDKHPDYNTWGAYQCYG
                     DYFYKLTGKSSMRVADAPFVTESHAIIELQNEISLISGRKVDKPIHMRILEGLKARVD
                     AGNVSSGAVKELFANAYALLGEYKVAVELYSKLREVEAADFSVSSLEQYCSLRGKIIL
                     EDFKAGRGTDEAGIERLIEDMRLIDLIGNTSERKALKGSLYKRLSYIRKEDAKKKRSC
                     LGLMKYFFGEAFCLMRSMEEADRVYSLTNYLLAAYLEKGRIEWKDIQKLGIQGYSEVI
                     EMLNGYADRLKSDSEKYKDYYRANALAKIYLVRMLYIDKQESLDQEITIYRKALMDSL
                     VSFANLKQILGEVEFLDYITGMLPKKDKKKIEALIKVKVDVASLLT"
     gene            complement(3718..4386)
                     /locus_tag="PLG24_07180"
     CDS             complement(3718..4386)
                     /locus_tag="PLG24_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HMZ24709.1"
                     /translation="MAHKVKIKSIAPITHDVLRIVAERPEGLIYTPGQAVDISINQEG
                     WDKEIRTFTFTSLPSDDSIEFTIKTYQAHNGVTNYLRELHAGDEINLYDVYGDIHYAG
                     EGIFIAGGAGITPFIAILKQLEAEGKLGGNKLIFANKTKADIIDADRFEKLLGDKFIN
                     VLSDEKIPGYEHGFITAELIRKNSDPQRHYYYLCGPPPMMDAVQRHLASLGIAESHII
                     KEGF"
     gene            complement(4403..5038)
                     /gene="folE"
                     /locus_tag="PLG24_07185"
     CDS             complement(4403..5038)
                     /gene="folE"
                     /locus_tag="PLG24_07185"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HMZ24710.1"
                     /translation="MQKTIPLNHREVSSETKHSPEFNGLTDEEKIRKIKYHFAQIMDA
                     LGLDLSDDSLMDTPERVAKMYVKEVFSGLNPANKPVISLFDNKYQYNKMLIEKDITLF
                     SYCEHHFVPIIGRAHVAYIPDDKIIGLSKLNRIVQYYAKQPQVQERLTIQIADALKEI
                     LQHDNVAVMIEADHLCVASRGIKDTNSKTITSMLSGRFEEDSVRNEFLSYV"
     gene            complement(5042..5689)
                     /locus_tag="PLG24_07190"
     CDS             complement(5042..5689)
                     /locus_tag="PLG24_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ24711.1"
                     /translation="MVIHDILNEKEIISRILNGEKSLYEIIVRRYNPFLYKLGRAYNY
                     NHDDTQDLMQDTYIDAYKNLGQYKGIANFKTWLFRIMLNNCFRKKEKLSYKNEVMQDI
                     NDNSKPLFSHSGHDTERIVHNKELGEIIEKALSEIPFDYRMAFSLREINGLNVAETAE
                     LLDISEANVKVRVNRAKNMLREIIEKNYIGEELFAFNLIYCDAMVNNVMNIVNEL"
     gene            complement(5872..6252)
                     /locus_tag="PLG24_07195"
     CDS             complement(5872..6252)
                     /locus_tag="PLG24_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24712.1"
                     /translation="MATYNETIIKKTTHLLRYTFTILPIAAGTDKFLNMLTDWTAYIN
                     PGFEEILPFSGSVFMMIVGVIEIIAGIIVFTKPKTGGLVVAAWLIIIALSLLAGWNHV
                     DVAVRDLVMAIAAFSLSNLVKASE"
     gene            complement(6394..>6652)
                     /locus_tag="PLG24_07200"
     CDS             complement(6394..>6652)
                     /locus_tag="PLG24_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24713.1"
                     /translation="TALNDWYKNAQDIADFLANANPNWKQEDMRHMMKTHIDQTTEYA
                     VDLLQKDYSKAIKVFDEANEHMLMMADDLSEGIAKQFPEKF"
BASE COUNT         1869 a         1277 c         1516 g         1990 t
ORIGIN      
        1 aagacctgac agaatctgcc aggctgcttg ctgcggagtt gaagcgatgc ctcagtttcg
       61 gaaagccggt tcaggtgatt gctcattcca tgggcggttt ggttatgaaa caactgatga
      121 tcaaagaaaa atcgacctgg cattcatttt gccaatcgtc atcgaatcgc ttaatactcc
      181 tgggtacgcc atggcttggt tcacatctga tcatggaagt gttgaccggg cattcatcca
      241 aggttaagtt gctgggtctt ttggacaaaa ttcatgatag agaacagatt atgtccactg
      301 ccgccggttt ccccggagtt atccagttac tgcctatgac ggacgaaggc tttggcactt
      361 cagtcttttg ggagaatttc agaccttata ccaatcatct ggctgtccct ccttcacata
      421 tgtttgattt atataataat tacaagcagg aaactgccgg cttcaggatg gatgaagccc
      481 acctggataa tgtcttttat attgccggcc atgatgaaac aacctccagc tataaaatcg
      541 aatggaagtt tctacaaggt aaaaggttgg tctttaggtc aacagatgcc ggtgatggca
      601 gcgtaacctg gaaattgggc attcctcccg aactaaaaag gtccaacctt tattacagtg
      661 aaatcacaca tggcgaatta gctaacaagc agatcatcat tgacacaatc atggacatat
      721 ttttgtccgg aaaatcttcc agactacccc aaactgaacc tggcagtcgg ggtattcaac
      781 aagaaggcat tgtacccgag tttgatttta tacccaacaa tgatgatgag ttggttgatg
      841 ctctcttcca gggcaagatt ggccgaaggg aggcgaagcc acagactgaa ccattgcagg
      901 tgtcggtata tcatgcggac ctgaaatatt cgtcccatcc ggtattggtc ggacattttc
      961 tcaatgatgg tctttatagc gctgaaaaaa gtctggacag ttaccttggg aacaagctaa
     1021 gtgagagact tcggcttggc tattatcccg gcaatatcgg tgaatgcgag atcacatatc
     1081 aaagtgaatc aaggccaaaa ggcgcattag ttgccggttt aggattagct gatgaattaa
     1141 catcgtatcg gctttcccta accgtcgaga gtgccatcat caaatacgca tttttcttta
     1201 gggacaatta cagcaggagt gagaataaga gggtagcatc cgggatatct actgtactgg
     1261 taggttcatc atttgcaggt attccgatta acgaatgcct ccgggctatt ttgtcaggta
     1321 ttcaaagagc taatgaaaga atggttcaac ttaatatcgg gcttcagttg atccggcagg
     1381 tggaatttgt cgattattat gaagatctgg cacaacaatg ctacagtatt ttgaaggatt
     1441 tcgagcggaa aagcgatgat ttcaatattg aggttgcacc attcagaaag ggacccggga
     1501 tgaggcgtcg gcttaccctc ggcgatacat cgagctggtg gcggactttc aatacagtaa
     1561 ctaattttga tcggcttacc ggacgaccat ctggctttac tttcagcctc acctccggac
     1621 gcgcaagaat agagcagggt ggggtgacta ccgatctgaa gctggttgaa tatctggcaa
     1681 aggaatattc tcataagaac gattggaatc ccaggttgtc taaaactatg tttgaacttc
     1741 ttatcccaaa tgatttcaaa aacatcatcc gcaaccaaag caacatggta tggaaactgg
     1801 atgaatacgc ggctcaattt ccatgggaat tgttccacga ttacaatgac ataaccaatg
     1861 cacaccggga aaaacctgcc tttgtcaata ctggtatgat aaggcagttg attacagaag
     1921 atttccggcg taacccgaca gttgttactg aggataaggc tctggttatt ggggatcctg
     1981 attattcggg gactgtattt tcgcaacttc agggagcaga agaagaggca agggtaattg
     2041 gaaaaaagtt ggttgaggat aattttgaag tcagatcgat gattcattcc cgggcgggag
     2101 atatcatcac tgagttgttt tctgacaaat acaagataat ccacattgcg gcgcatggtg
     2161 tatatggaga agtggctgtg aatgattcga aggaattcaa ggccggggtc gtattaggca
     2221 acgatgtata tctggaggct agcatgatca accagctatc gacaatacca gagtttatat
     2281 ttattaattg ctgttattct ggcgacatga ccggattgga tgaaaagtat tttaaggaaa
     2341 ggtctgcgct tgcagccaac atcggtacac aattggtacg aatgggtgtc aaggcggtaa
     2401 ttgtcgctgg ttgggctgtt catgatggtg ctgcattgac attctcgacg gagttgtacg
     2461 acaggatgct gagtggtttt gagtttggag aagctgtact ctctgcacgc cgtaaatgct
     2521 atgacaagca tccggactac aacacctggg gagcatatca atgctatgga gattatttct
     2581 ataagttgac aggcaaatct tcaatgcgtg ttgcagatgc cccttttgtg acagaatcac
     2641 atgccatcat cgaacttcag aacgagattt ctcttatttc aggcagaaaa gtcgacaagc
     2701 caatacatat gagaatcctg gaaggcttga aggcgagggt cgatgctggt aatgtttctt
     2761 ccggggcagt caaggaattg tttgcgaatg cctatgcctt gttgggagaa tacaaagttg
     2821 ccgtggaatt gtatagtaaa ttaagggagg tagaagcggc agatttttca gtatcatctt
     2881 tggagcaata ttgcagtttg cggggtaaga tcatcctgga ggattttaaa gccggcaggg
     2941 gcacagacga ggccggcata gaaaggctga ttgaggatat gcggttgatt gacttgatag
     3001 gtaatacgtc tgaaagaaag gcactgaagg gctctcttta caagaggctt tcctatatcc
     3061 gtaaagaaga tgctaaaaag aagagaagct gtctgggcct gatgaagtat tttttcggtg
     3121 aagctttttg cctgatgagg agtatggaag aagctgatcg ggtttattcc ctgactaatt
     3181 atctgttggc ggcctatctg gaaaagggca gaatagaatg gaaagatatt cagaagctcg
     3241 gcatacaggg ttattcagag gtcatcgaaa tgctgaatgg ctatgctgac agacttaagt
     3301 cggacagtga aaaatataaa gattattaca gggctaatgc attggccaag atatatcttg
     3361 taagaatgct ttatatcgat aaacaggagt ccctagatca ggaaatcaca atatatcgaa
     3421 aggcattaat ggattctttg gtcagttttg ccaatctgaa gcaaattttg ggtgaagtgg
     3481 agttccttga ctatattacc gggatgctcc ccaaaaagga taaaaagaaa atcgaggcat
     3541 tgatcaaggt aaaggtggat gtggcaagct tattgactta gacgttttct gcaactactt
     3601 ctatcaacat caactgccaa gcagcatctg gcagcaaagg gaaaattgaa ttaatggtac
     3661 ggaaaaccga ctcaatacaa gatcagtaat actataccgc ctttagtcag tcagagtcta
     3721 gaaaccttcc ttgataatgt ggctttcggc aattcctagt gaagcgagat gacgctggac
     3781 agcatccatc ataggcggtg gaccgcacag gtaataataa tgcctttgcg gatctgaatt
     3841 ttttctgatt aattcagcag taataaatcc gtgttcgtag cccggaatct tctcatcaga
     3901 taaaacattg atgaatttat cccccagcaa tttctcaaaa cgatctgcat caatgatgtc
     3961 agctttggtt ttgttggcaa atatcagttt attgccacct agtttgcctt cagcttccaa
     4021 ttgtttcagg atggcgatga atggagtgat accggcacca ccggcgatga atataccttc
     4081 accggcataa tggatatctc catatacatc ataaagattg atttcatcac ctgcatgaag
     4141 ttcacggagg tagttggtga cgccgttgtg tgcctgatag gtcttgatgg taaattcgat
     4201 agaatcatcc gaaggcaggg atgtgaaggt aaaggttctg atctctttat cccatccttc
     4261 ctgattgatc gatatatcga cagcctgccc cggagtgtat atcagacctt ctggacgttc
     4321 tgccacgatg cgcagtacat cgtgtgtaat aggggcaatt gattttattt ttactttatg
     4381 ggccatttta cttaattttt atttatacat aggataaaaa ctcatttctt acggaatcct
     4441 cctcaaatcg gccggaaagc attgatgtga tcgttttact attggtgtcc ttgatgcctc
     4501 ttgaagccac acaaagatga tcggcttcta tcattactgc tacattgtcg tgttgcagga
     4561 tttccttcaa cgcatcggca atctggatgg taagccgttc ctgaacctgc ggctgctttg
     4621 cataatactg gacgatacgg ttgagcttgg atagtccgat gatcttgtca tcaggaatat
     4681 aggccacatg tgctcttcca atgataggta caaaatggtg ttcacaatac gaaaacaaag
     4741 taatatcctt ttcaatcaac attttgttgt attgatactt attgtcaaac agactaatta
     4801 cgggcttatt tgccgggttc aatccgctga atacttcctt gacatacatt tttgcgactc
     4861 tttctggagt atccataagg ctgtcatccg aaaggtccag ccctaatgca tccataattt
     4921 gagcaaaatg gtatttgatt ttcctgattt tctcttcatc ggtcaatccg ttgaattctg
     4981 gtgaatgctt tgtttcggat gagacttctc tatggtttaa tggaattgtc ttttgcatga
     5041 cttacagttc gtttacaata ttcataacgt tgttgaccat agcgtcacaa tatatcaggt
     5101 taaatgcaaa cagctcctca ccgatataat tcttttcaat gatttcgcgc agcatgtttt
     5161 tagcacgatt gacccttacc ttaacattcg cttccgagat atcaagtaat tcggccgttt
     5221 cagctacatt caacccattg atttcacgaa gggaaaaagc catcctgtag tcgaaaggga
     5281 tttcgctgag tgctttttcg ataatttctc ccaattcctt gttatgaaca atcctttctg
     5341 tatcatggcc ggagtgggaa aatagcggtt ttgagttatc atttatatct tgcattactt
     5401 catttttata gcttaatttt tccttttttc gaaaacaatt atttaacatg atacggaaaa
     5461 gccaggtctt gaaattggct atgcctttgt attggccaag atttttgtag gcatcgatat
     5521 aggtatcctg cattagatcc tgtgtatcat cgtggttgta gttgtaggcg cgccccaatt
     5581 tatagagaaa agggttgtaa cgtcttacta tgatttcata taaagatttt tctccgttga
     5641 ggatccgtga tataatttct ttttcattca atatgtcgtg tattaccatt ttaccccgta
     5701 aaattttagt ttgagtaaca ttaatatgtt tattgtcagg taaatatttt attctcccga
     5761 tatgtttata agattagagt aaatgggaaa gaaaaaggtt acaaaaaatg taatttattt
     5821 tttgtggatt tttgtaacct cttctaaatg aataatatgg atctttataa tttattctga
     5881 tgcctttacc agattagaca gtgaaaatgc agcaattgcc atgaccagat ctcgaactgc
     5941 cacatcgaca tgattccaac cggcgagtaa ggacaatgct attatgatca gccaggccgc
     6001 aacgaccaga cctcctgttt ttggtttggt aaatactata attccggcga tgatttcgat
     6061 tacgccaact atcatcatga aaaccgatcc ggagaaggga agtatctcct caaagccggg
     6121 gttgatgtat gcagtccagt cggtaagcat gttcaggaat ttatcggtac ccgccgcgat
     6181 aggtaggatg gtgaaagtgt atctcaataa atgggttgtt ttctttatga tggtttcgtt
     6241 atatgttgcc attttgttgt tacttattta ggttagaaaa acaccgggga aattcaacca
     6301 ttatccccgg ccggatacta gatgtgatca attcactaag agaaagattt attcgaacaa
     6361 ggaagtcgtg aattttctct gaatagatag cgattagaat ttttcaggga attgtttagc
     6421 aataccttca ctgaggtcgt ctgccatcat aagcatatgt tcatttgcct catcgaaaac
     6481 tttgattgct ttgctgtaat ccttctgaag caggtcaaca gcatattctg tagtttgatc
     6541 aatgtgtgtc ttcatcatgt gccgcatatc ttcctgcttc cagtttggat tagcattagc
     6601 aaggaagtct gcgatatcct gagcattctt gtaccaatca ttcaaggcgg tt
//
LOCUS       DAOQQN010000162         6628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4896_length_6628_cov_5.218013, whole genome shotgun sequence.
ACCESSION   DAOQQN010000162 DAOQQN010000000
VERSION     DAOQQN010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4896_length_6628_cov_5.218013"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3219)
                     /locus_tag="PLG24_07205"
     CDS             complement(<1..3219)
                     /locus_tag="PLG24_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HMZ24714.1"
                     /translation="MRTISFLFSLIFFVTLTSCFSNLKQKDVGGTGNAISESTFDNEN
                     NENMANLSNPTDPKDFVSVDSLISIRQYKSAEKLLGTLLTKYKSLGQWDGYIRAVMTL
                     ARIKSENENGTKDAIAYLESEVASATFPARQILLNVTAHTYASYLNANRYQLQDVTSG
                     EGLQEDDDFETWSKEKLLEKIDLYYDLSIADEKLRTIPIRDIDLITTQSNADLYRPSL
                     YSFLAHQMLDYLADSRNYIEEPVYKFEIRGSEWFAPYEEFIAMKLEAKDSTSRVFHSL
                     KIYQQLLQHHLSNGNEEALRRIDLERLNFVYEHSVDPLKDRDFKVAIQKQVESGAKQD
                     WKAAFEYRLIELELAIDKYSSDNPDQGDRFLLNKMIERLKKLSSDYPNNKYGIKAQNK
                     IASLQARSVSIQGESVQFPGKPFLLAVQYANLTEAEVNIYKLTREQFFQFQFNRYERN
                     SLQEITGKNLFKTVKIDLPDFADHRSHRVEVKMDGLDQGIYLFVPQKLPDNGEYYSET
                     AVVQFSNLAVMRNNPYGTLDHSTVVIDRMSGMPLEGVKTEIYTYSYNTRNIRLLNESK
                     SDKNGFVTPKVSPDNNYFASFTKGGDQLIDLRQSYPYRFGGRHDRSINYNVFTDRAIY
                     RPGQIIYFKAILYSHDEKSVPHIIPNKKVTVSLRDVNNQEVSKGTFVSNEFGSLHGNF
                     TVPSGVLTGVMSIYIDGQHTKGIQVEEYKRPKFEVKIDTITQAYVLNDKVRISGKALN
                     YAGNAVDGAKGVFTVTRRQIWRYYFWNFDYFRPSFPSNEMIIANGEITTETDGSFTIV
                     FDALADESISKEIKPKFSFDISVDITDINGETQSGSTNVLLGYDPYNTDYNIAGEVEV
                     KDLRNLVVDTKNSAFVSVPTNGRLRIELLTSSNQYLRNKLYAYPERVTISREEYKKLW
                     PYESYLNEADRTTWSVKKTIFDRKIKSGTGPVDALKSVDFEPGDYRIVFDVDGMNPVS
                     NKEALYVSVKADGSKGEFALDRFIFAATDKPSYQVGMVSNQTYKTRNTSGKLLYEFYF
                     QGKLLSREWLNAASALGKTLKINEEYRGGVTVIASAYGFGRAQ"
     gene            3989..4312
                     /locus_tag="PLG24_07210"
     CDS             3989..4312
                     /locus_tag="PLG24_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24715.1"
                     /translation="MMTQRMTDLMKLLENDPNDSFLLFAIAKEYEQMSMPDQAEHYYL
                     TLRKANPSYVGLYYHLARLYEDTGKVEDALNIYDEGIFMANKMRDQHALAELKNAKMN
                     LELMM"
     gene            4583..5659
                     /locus_tag="PLG24_07215"
     CDS             4583..5659
                     /locus_tag="PLG24_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HMZ24716.1"
                     /translation="MVQQFYSLEIDFLEHQTEDSTVIGFNTKEVPEGVFDFKAGQNIT
                     IKYYFDGEEIRRSYSICTCPSESRIHIGVRAIKDGLFSNFALKNLKVGDRLDILPPVG
                     SFTIQGKMATSGRYVFFATGSGITPVISMIKDKLEKDPAATCTLFYSNRSVGSMMFRD
                     ELDGIKNKYSTRFQAVYLFSREQLDSDLLYGRMDGDKVRQLMNIFVADKAGSQYFICG
                     PEEQTIAIRNLLTKELGIPSGQVHFELFHTGDLKPEVKSQEEVTDFKGTAEVTVRIDG
                     KETTFHLAYDKESILDAGIRQGLDLPYSCKGGVCCTCRAQLLEGKVHMDQNYALEDDE
                     VEEGFILTCQSHPRAGKLFVDYDV"
     gene            5666..6256
                     /locus_tag="PLG24_07220"
     CDS             5666..6256
                     /locus_tag="PLG24_07220"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ24717.1"
                     /translation="MKQEGNNPRRDYIILKAAEIMRNQGYAGATMRDIAREVGMEVSS
                     LYNHIRSKHDILATICFSIGDEYITGLDTILDRETAYPEKLSCIIGLHIDLAARHKDL
                     MIVFEEEWKHLQDEDLNKFKSMRAYYEKKLENFFRLGMEQGKFKNMDDRTMLLTFLSS
                     IKWLNYFFKDKNNYDIAVLKREVMALISHGLLADPH"
     gene            6291..>6628
                     /locus_tag="PLG24_07225"
     CDS             6291..>6628
                     /locus_tag="PLG24_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaI"
                     /protein_id="HMZ24718.1"
                     /translation="MSIAASANHFQDRIDRDEKIEPNDYMPEEYRKTLIRQISQHAHS
                     EIVGMLPEGNWVTRAPTLKRKAILLAKIQDEAGHGLYLYSACETLGVDRDDLIDELHE
                     GKAKYSSIFN"
BASE COUNT         1970 a         1366 c         1361 g         1931 t
ORIGIN      
        1 ctgtgccctg ccaaatccat aagcagaagc aataacagtg acacctcccc ggtactcttc
       61 attgatcttc aatgtttttc ccaaagctga tgcagcattc aaccactcgc gagaaagaag
      121 ctttccttga aaatagaact cataaagcaa ttttccggaa gtattcctcg tcttgtatgt
      181 ctgattactc accattccta cctggtagct cggtttgtca gttgcagcga atataaacct
      241 atccagggca aattctccct ttgagccatc ggctttcacg cttacataca acgcctcctt
      301 gtttgatacc ggattcatac catcgacatc gaagacaatg cggtaatcac ctggttcgaa
      361 atccactgat ttcaatgcat cgacaggacc ggtgccggat ttgattttcc ggtcgaaaat
      421 agtttttttg acagaccatg ttgtgcgatc cgcttcattc agatagctct cataaggcca
      481 gagttttttg tattcttccc tgcttattgt cacacgctcc gggtatgcgt atagcttatt
      541 cctcaaatat tgatttgaag aagtgagtaa ttcgatacgc agcctgccat tggtcggtac
      601 agagacaaaa gcactattct tcgtgtccac caccaggttt ctgagatctt ttacctcaac
      661 ttcacccgca atattgtaat ctgtattata tggatcatat cctaacagaa catttgtact
      721 gccggattgg gtctctccat tgatatctgt tatatcaacg gatatgtcaa atgaaaactt
      781 cggcttgatc tctttactaa tggattcatc cgccagtgcg tcaaatacaa ttgtaaacga
      841 accgtcagtc tctgttgtaa tctctccatt ggcaataatc atttcattgg aggggaaaga
      901 aggcctgaaa taatcaaaat tccaaaagta ataacgccaa atttgtcgtc gtgtaacggt
      961 aaatacgcct tttgctccat caactgcatt tcctgcgtaa ttgagtgcct tgccacttat
     1021 tcttactttg tcattgagta cataagcctg agtgatggta tctatcttta cttcaaattt
     1081 aggccgcttg tattcttcaa cctgaattcc tttagtgtgt tgtccatcaa tatatatgct
     1141 catgacgccg gtcaagacac cggaaggtac tgtaaaattg ccatgcaagg agccgaactc
     1201 attggaaaca aaggttcctt ttgatacctc ctgattattg acatcacgca gactgacagt
     1261 caccttttta tttggaatga tgtgaggaac agacttctca tcatggctgt acaggatagc
     1321 tttgaaatag ataatctgtc ccggcctgta aatagcccgg tccgtaaata cattgtaatt
     1381 tatagacctg tcgtgccgac caccaaatct atagggatag gattgtcgga ggtcaatcaa
     1441 ttgatctcca cctttggtaa atgatgcgaa atagttgttg tccggtgaga ctttaggtgt
     1501 gacgaatccg ttcttatcag acttgctttc attgagtaac ctaatgttac gtgtattgta
     1561 gctgtatgta tagatttctg tcttcacacc ttctaagggc attccactca tacggtcaat
     1621 gaccactgtc gaatgatcca atgtgccata gggattgttt ctcattactg ccaggttaga
     1681 gaattgcacc acagccgttt cactataata ttctccattg tcggggagtt tctgtggtac
     1741 aaagaggtaa ataccctgat cgagcccgtc catctttact tccacacgat gagaacgatg
     1801 gtcagcaaag tccggcaaat ctatcttcac cgttttgaag aggttcttac cggttatttc
     1861 ctgcaatgaa ttcctctcat atcggttgaa ttgaaattga aaaaattgtt cacgggtcaa
     1921 tttgtaaata tttacctcag cctcggtaag attggcatat tgcacagcca gcagaaaggg
     1981 tttgccgggg aattggacac tttccccttg aatcgataca gagcgggcct gaaggctggc
     2041 aattttgttc tgcgccttga tgccgtattt attgttgggg tagtccgaag aaagcttttt
     2101 caacctttca atcattttgt ttagcaaaaa ccgatcccct tgatcaggat tatcggaaga
     2161 atatttatcg atggcaagct ccaattcaat cagcctatat tcaaaggcag ctttccagtc
     2221 ctgtttagct cctgattcaa cctgtttttg tattgccact ttgaagtccc tatccttcag
     2281 gggatccacc gaatgctcat atacaaaatt caacctttcc aggtctattc tccgtaaagc
     2341 ctcctcattg ccgttgctca gatgatgttg caacaattgt tggtagattt ttagactatg
     2401 aaatacccgc gatgtagaat cttttgcctc gagcttcatg gcgataaatt cctcatatgg
     2461 agcaaaccat tcactgccac ggatttcaaa tttatatacc ggttcttcaa tataatttct
     2521 gctgtcggca agatagtcca gcatctgatg agccagaaaa ctatatagtg aaggcctgta
     2581 taggtctgca ttactttggg ttgtaatcag gtcgatatcc cggataggta tggtcctgag
     2641 cttttcatcg gctatggaaa ggtcatagta taagtcaatt ttttccaaaa gtttttcttt
     2701 agaccaggtc tcaaagtcat catcttcctg caggccctca ccggatgtta catcttgaag
     2761 ttggtaccgg ttggcattca gataggaagc ataggtatga gcggtgacat tcagaagaat
     2821 ctgacgagcc ggaaaagtgg cggaagcaac ctcactttca agatatgcta tggcgtcctt
     2881 ggtgccattt tcattctcag actttatcct ggccaaggtc atgaccgcgc ggatatagcc
     2941 atcccactgt cctaaactct tgtatttagt caacaaggtg cctaacaatt tttcggcact
     3001 cttatattga cgtatactga tgagactgtc tactgaaaca aagtcttttg gatcggtagg
     3061 attggataga ttggccatat tttcgttatt ttcgttatca aatgtacttt cagaaatagc
     3121 attaccggta cctccaacat ccttttgctt cagatttgaa aaacaacttg tcagggtgac
     3181 gaaaaatatc aggctaaata aaaaactgat agttcgcatg gttgtattat taaggttgaa
     3241 caatgattgt ccggttataa atccattttc ttttccataa cgtaatttta agtcataaag
     3301 ttattgaaga atgaaacttt ctcaaaaaac attttaattt actaaaaaac tttttcattt
     3361 aaaaaaaatg aaaggactac caagcttgat ggtgttcact ttggcaggcc ttcctgatag
     3421 attcccggaa actattcatt tgattaataa caatatcatc gtaggttaat cagattgaag
     3481 gtacaaatac caagcggcat tattgcagga aaatttgttc tgatcaaact tgagcaacac
     3541 tgatttttat ttaaagcgaa aaaaggaaga cgcagaagga tttttgcttc ttttatttca
     3601 ttaagtgatt catattcaac tcaatacacc catattgagt gtatccgttc ttcaaactgg
     3661 ttcataaaac tatttggcga aattaatcag gttgataatt actttagtga aattttcatg
     3721 ggtatcaagt agtaaacccc tcataatcag gtagcaatta taagaaatca ggcaaggtta
     3781 accagattcg ttacaaataa tatgagataa aacagaaatg tgattattat ttcttatatt
     3841 tacgggaaca aggaaaaata gtcattcgga tcaaaggcaa agaaacagat tgtgaaacaa
     3901 ctattcacta aacgacaaaa aaggtaggtc gctatcattt ttgtgtgaat gattagatct
     3961 gtttattgtt aaagaaaaat gaagagatat gatgacgcaa agaatgactg atctgatgaa
     4021 gcttttggaa aatgacccca atgacagctt ccttttgttt gccatagcca aggaatatga
     4081 acaaatgagc atgcctgacc aggcagagca ttattacctg acactccgta aggccaatcc
     4141 ttcctatgtg ggactttatt accacctggc caggttatat gaggataccg gaaaggtgga
     4201 ggacgccctg aatatatatg acgaaggaat atttatggca aataagatgc gcgaccaaca
     4261 tgcccttgct gaattaaaaa acgcaaaaat gaacctcgag ttgatgatgt agtgattcag
     4321 ctcttctcaa cttttgcagc attgctgggt tactcttacc cggaaatcca atacattgtg
     4381 gatgttccta aacgacattt tttactgacc cggtcatcca atttcttaat gagtccgtaa
     4441 aacgactttt caattgaatc cagacaacct gctttctctc cttaaaaaca atcaagaaca
     4501 ttttcgcgct aaacttgttc gacctacgaa caattgttcg catatcatca tatttttcta
     4561 tttttgcgca aaaattcaac cagtggttca acagttttat tcactcgaaa tagattttct
     4621 ggaacatcag acagaagaca gtacagtcat aggtttcaat accaaggaag tacctgaggg
     4681 cgtattcgat tttaaagcag gacaaaacat caccattaaa tattatttcg atggggagga
     4741 gatccgcaga tcatacagta tctgcacctg tccttcagag agtcggatcc atatcggagt
     4801 aagggccatc aaggacggtc ttttttccaa cttcgctctt aaaaatttaa aagtcggtga
     4861 cagactcgat attcttcctc cggttggcag tttcacgata caaggcaaga tggcaaccag
     4921 cggccggtat gtattttttg ccacaggaag cggcatcaca ccggtgattt ccatgataaa
     4981 ggacaaactg gaaaaagatc cggctgctac ctgtacctta ttttacagca accgttcggt
     5041 aggttcgatg atgttccggg atgaactgga cggtatcaag aataaataca gtaccagatt
     5101 tcaggctgta tatctgttca gccgagaaca actggattcg gatctcctgt acggaaggat
     5161 ggatggagac aaggtgaggc aattgatgaa catctttgtt gccgataaag caggtagcca
     5221 atattttatt tgcggaccag aagaacagac cattgccatc aggaatctcc tgacaaagga
     5281 attgggcata ccttccggtc aggttcactt cgaacttttc catacgggcg acctaaagcc
     5341 ggaagtaaag tcacaggagg aggttaccga cttcaaaggt acagccgagg tcacagtcag
     5401 gatcgatggt aaggaaacca ccttccatct cgcttatgac aaagagagca ttctggatgc
     5461 cggaataagg cagggcctgg atctcccata ttcctgtaag ggaggtgtgt gctgtacatg
     5521 cagggcacaa ttgctggagg gcaaggtaca tatggatcag aattatgcat tggaggatga
     5581 tgaggttgaa gaaggcttta tcctgacttg ccagtcacat ccgcgtgcag ggaaattatt
     5641 cgtcgattat gatgtataat ggtgcatgaa gcaggaaggt aacaatccgc gccgggatta
     5701 catcatcctt aaggcagcag aaatcatgag gaatcagggt tatgccggtg ccaccatgcg
     5761 cgatattgcc cgggaagtag gtatggaagt cagcagtcta tacaaccata tccgctctaa
     5821 acatgatatc ctcgctacca tttgcttttc aatcggggat gaatatatca caggtcttga
     5881 caccatcctg gatcgggaaa cagcttatcc cgaaaagctt tcctgtataa tcgggctaca
     5941 tattgatctg gcagcaaggc acaaggactt gatgattgta tttgaagaag aatggaaaca
     6001 tttgcaggac gaagatttaa ataaattcaa aagcatgcgt gcttattatg aaaaaaagct
     6061 ggaaaacttt ttccgattgg gaatggaaca gggaaaattt aaaaacatgg acgaccggac
     6121 catgctgcta accttccttt cttccattaa atggctcaat tatttcttta aagacaaaaa
     6181 taattacgat attgctgtcc tcaaaagaga agtcatggcc ttaatcagtc atggattgtt
     6241 ggccgacccg cactaataca aattaaaatt aacaattcaa attaaggaat atgtcaatag
     6301 ctgcatccgc aaatcatttt caggacagga tcgaccggga tgaaaagata gaacccaatg
     6361 attacatgcc ggaagaatac agaaagacac taatcagaca gatttctcag cacgctcatt
     6421 cagagattgt aggtatgcta cctgaaggta attgggttac aagggcccct acattgaaga
     6481 gaaaggccat cttgctggcc aaaatccagg atgaagcagg acacggactt tacctgtata
     6541 gcgcctgcga aacactgggt gtggacaggg atgatctaat agatgaattg catgaaggaa
     6601 aggctaaata ctccagtata ttcaacta
//
LOCUS       DAOQQN010000163         6605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4924_length_6605_cov_4.366718, whole genome shotgun sequence.
ACCESSION   DAOQQN010000163 DAOQQN010000000
VERSION     DAOQQN010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4924_length_6605_cov_4.366718"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLG24_07230"
     CDS             <1..239
                     /locus_tag="PLG24_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ24719.1"
                     /translation="GIQCFPNPAHNDLVINVNSLKFKGLSWRIIDVSGKLILKGELNA
                     PYQRINIAGLTAGAYRMLISDSQHVVSVHSFSRI"
     gene            282..998
                     /locus_tag="PLG24_07235"
     CDS             282..998
                     /locus_tag="PLG24_07235"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HMZ24720.1"
                     /translation="MIDPQELKKRIVFEDNHLIAINKRPSWLVQGDKTGDMPLLDALK
                     DYIKKEYNKPRDIFLGSIHRLDRPVSGLVVFARTSKALERMNALFAGREVVKTYVAIT
                     EGKPPVSVGTLRHFLLKDNHNNTVNVVNPSRKGLKDVKEAILDYVEVNRHGLHTLIKV
                     VPHTGRPHQIRVQLASMGCPILGDVKYGARAPLSDQSIALHSFSLQFVHPVRKEALCL
                     VAPLPETAWWKPFDTDPLNK"
     gene            complement(1135..2910)
                     /locus_tag="PLG24_07240"
     CDS             complement(1135..2910)
                     /locus_tag="PLG24_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ24721.1"
                     /translation="MDTTIEKFKGGEFLIKDYSFGSDYIPERMNEDQKMIRDTVREFV
                     EKELWTRGSKLEEQPALMDEAGELGLVGAHIPAEYGGQEFDTIINTILLEELGGGDAS
                     FSTTFAAHTGIGMLPILYFGTEEQKKKYLPELSSGKVRAAYCLTEPGSGSDALGAKTK
                     AILNDAGTHYLLTGQKMWISNAGFADVFIVFAQVDGDKFTGFIVEKGTEGMTLGEEEH
                     KLGIKGSSTRQIFFENAPVPKENVLGEIGKGHLIAFNVLNMGRFKLGVLVGGGCKVTT
                     TRAVNYAMEREQFKTPIGRFGAIQHKMAQMAINAYVLESTNYRIANLIEDWKASAIED
                     GVPYQQALLDAAEEYAIECAITKIYGSECIDYNVDELLQIHGGYGFSEEFVPAGLFRD
                     TRINRIYEGTNEINRLLLVNMLLRRVMKGQLDMVGPAWEVQKELASMPKMESFEGAYP
                     EETKALKNFKKILLMVAGAGAKYQMDGKVDLKEEQEILMNIADIMIDVFNAESVLSRL
                     KEVKNMDDGLLEEKEAMLKVFFHDAQDRIAKNAVDALASFATGDELKIMLMGVKRYSK
                     LEPVNVKNLRKKIAERIIEAGRYPF"
     gene            complement(3251..4147)
                     /locus_tag="PLG24_07245"
     CDS             complement(3251..4147)
                     /locus_tag="PLG24_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HMZ24722.1"
                     /translation="MAPSQVRPKSAIIKSQIRSALLIILGIFSAGFGLESFLVPNGYI
                     DGGITGISLLTKVIYNKLPLSVLLIVYNTPFILLGFRQVSKEFALKSIIAITGLAIVI
                     QFVHFPILTDDPLLIASFGGLFLGAGIGFAMRGGSVLDGTEVLAIYLSRKSMLSIGDI
                     ILIFNIIIFLTAAYLLSIDIALYAILTYFVASRAVTYLIEGIEEYTGVTIISDNSEEI
                     REMLVHDLRFGVTLYSAKKGYGKRGHQSTEQIDVVYTVVTRLEISKLQDGVKAIDPSA
                     FIIMSSVKDTVGGMIHRRGGVH"
     gene            5022..>6605
                     /locus_tag="PLG24_07250"
     CDS             5022..>6605
                     /locus_tag="PLG24_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HMZ24723.1"
                     /translation="MKKQDKKASPPVAAKRPIHLEKHGDIRVDSYYWLKEREDPEVIK
                     YLEDENTYMEQSLSHTKGLQDKLFGEITSRIPQIDMSVPYLKNGYYYYTRYESGKEYP
                     IYARKIDTLDAREQVLINVNEYAQGYNYYNIKQIAVDPFNKYMAYSEDTLSRRIYTIK
                     VMDMVTGKFTGDILHENSGNIVWFNDGKAFLYVVKDPETLREYKVYRHLVGDDSRNDK
                     LLYEEKDETFGVGIFKSRSNAFIYIGSYSTLTSEFRYCDANDSDAEFKLFELRKRDHL
                     YSVADDGRHFYIVSNDKAINFRLFKTDLDKTERSNWLEMIPHRKDVLIEDVECFNDFI
                     VVEERMKGMIRHRLISGEQDCYLDFDEDAYMTFLVDNHEFNTRKLRYSYTSLTTPLSV
                     FDYDLDTKEHILLKEQQVTGGYDKSLYTSERIEVIARDGTRVPVSLVYKKSLKKKRNN
                     PLLLYGYGSYGISIDPSFSSVRLSLLDRGFIYAIAHIRGGQELGRQWYEDGKLLKKNN
                     TFNDFVDCGRYLVTRGYTSP"
BASE COUNT         1886 a         1310 c         1312 g         2097 t
ORIGIN      
        1 atggcattca atgttttccg aaccctgccc acaatgatct tgtcataaat gtcaactctc
       61 ttaaattcaa gggcttatcc tggcgcatca tagatgtatc cggtaagttg atccttaagg
      121 gagagttgaa tgctccgtac caaagaatta atattgccgg tttgactgca ggagcatata
      181 ggatgcttat tagtgattct cagcatgtag tttcagtgca ttctttttcc aggatttgat
      241 tgcttcacgg gccggtaata tgaaagataa atcttctaaa agtgattgac cctcaggaat
      301 taaagaaaag aatcgtcttc gaggataatc atcttattgc gatcaacaaa cggccttctt
      361 ggctcgtaca aggcgataag acaggtgata tgccgcttct cgatgctttg aaggattata
      421 taaagaaaga gtacaacaag ccgagagata tttttttagg aagcatccac cgactggaca
      481 gaccggtttc aggattggtt gtatttgcgc gtacaagcaa agcacttgag aggatgaatg
      541 ctctttttgc cggccgtgaa gttgtaaaga cctatgtcgc gataacagaa ggcaaacccc
      601 cggtctccgt aggtactctc cggcatttcc tgttgaaaga caatcacaac aatactgtca
      661 acgttgtgaa tccttcccgc aaaggcttga aggatgtaaa ggaggctatt ttggattatg
      721 tagaagttaa caggcatggg cttcatacct tgattaaagt agtcccacat acaggcaggc
      781 cccatcagat tagggttcaa ctggcttcta tggggtgtcc tatactggga gatgtcaagt
      841 acggggcaag ggcgcctttg tcggatcaat ccatcgccct gcatagtttt tcccttcaat
      901 ttgtccatcc ggtaaggaag gaagcgttgt gtttggtcgc tccacttcct gagactgcct
      961 ggtggaagcc atttgataca gatcccttga ataagtaaaa gcattggatc cattttgatg
     1021 gttatagtgc tggtcagcat cgttaaataa aaaaaactcc ggaaatttcc ggagttttct
     1081 acaacgtaaa atctaaactt aataccttaa aaaaacatct ttatctggct gcagttagaa
     1141 aggatatctt cctgcttcaa taattctttc ggcgattttc tttcgtaggt tcttgacatt
     1201 gaccggttct agcttactat aacgcttgac ccccatgagc atgattttga gctcatcacc
     1261 ggtagcgaag gaggcaagtg catccacagc attcttggct attctgtcct gggcatcatg
     1321 gaagaatact ttaagcatcg cctctttttc ttcaagtaaa ccatcatcca tatttttaac
     1381 ctcttttaag cgggataaga cggattcagc attgaataca tcaatcataa tatcggcaat
     1441 attcatcaat atttcctgtt cctccttcag atcaactttt ccatccattt gatatttagc
     1501 gcctgcacct gcaaccatca gtaaaatttt cttgaaattt ttcaacgctt tggtttcttc
     1561 agggtaggct ccttcgaaag attccatttt tggcatggaa gcgagttcct tctgtacttc
     1621 ccaggcaggg cctaccatat caagttggcc tttcatgact ctgcggagca gcatattgac
     1681 caataaaagg cggttaatct cattggttcc ttcatagatt ctgttgattc gtgtgtctct
     1741 gaacaatcct gcagggacga attcttctga gaaaccgtat ccgccatgga tttggagtaa
     1801 ttcatcgaca ttgtaatcaa tacactcact tccatatatt ttagtgatgg cgcattcgat
     1861 ggcatactcc tcggcagcat ccagaagtgc ttgctgatat ggcacgccat cctcaatagc
     1921 cgatgctttc cagtcttcaa tcaagttggc tattcggtag tttgtagact cgagtacata
     1981 agcattaatt gccatttgtg ccatcttatg ttgaatcgct ccaaaacgac cgatgggtgt
     2041 cttgaattgc tccctttcca tagcataatt gactgccctt gttgtagtga ccttgcagcc
     2101 tccaccaacc aaaacgccca acttaaacct tcccatgttg agcacattga atgcaattaa
     2161 gtggcctttt ccaatttctc ccaggacatt ttcctttggt acgggggcgt tttcaaaaaa
     2221 gatctgccgt gtcgatgaac ccttgatgcc gagtttatgc tcctcctcgc cgagtgtcat
     2281 gccttctgtt cctttttcta cgataaatcc ggtaaactta tccccatcaa cctgtgcaaa
     2341 tacaatgaag acatccgcaa atcctgcatt ggaaatccac atcttctgcc cggttagcag
     2401 gtaatgcgtt cctgcgtcgt tcaaaatagc tttggttttt gcacccaaag catcacttcc
     2461 tgatcctggt tcagtaaggc aataggcagc acgtactttt ccggaggata attccgggag
     2521 atactttttc ttttgctctt ctgtgccaaa atataagatt ggcagcatac caataccggt
     2581 atgggctgca aaggtcgtac taaaagaggc gtctccgcca ccaagttctt ccagtaagat
     2641 tgtgttgatg atggtatcaa attcctgacc gccgtactct gccggaatat gtgcgcctac
     2701 taaacccaac tctcctgctt catccatcaa tgcaggttgt tcttccagtt ttgagcctcg
     2761 ggtccataat tccttttcta caaattcgcg aaccgtatca cgaatcattt tctgatcttc
     2821 attcatgcgc tcagggatgt aatccgagcc gaatgaataa tcttttatta aaaattcacc
     2881 acctttaaat ttttcaatag ttgtgtccat gtgttgatta aattacttat ttgtaaagtt
     2941 aacacatttt ggaggtaaat aattaaaaaa atgccaaaat tatttaatcg actatttcaa
     3001 tgcaatacat aataccgaaa acaaagaaat attatataat aaacaaaata atatacggta
     3061 gcttgtattt tacaaagtat aatgagagtc tacattagtg attaattccg agttgaattt
     3121 taattaaatg aatgtataat atttaacaga atagaataaa tattagaaaa agtaatttta
     3181 taataaggat ttttccttag acctcctctc attttcatag tgaaggtatg tccttttccc
     3241 ctaccaaaag ttagtgtact ccgcctcgtc tgtggatcat accaccgact gtgtctttga
     3301 cgctgctcat gattatgaat gcagatggat cgattgcctt tacaccatct tgcaatttgg
     3361 agatctccaa acgtgtcact acggtataga ctacatcaat ttgttctgtc gattgatgtc
     3421 ctcttttgcc atatcctttt ttcgcagagt ataaagtaac cccgaagcgc aggtcatgta
     3481 ccagcatttc cctgatttct tcgctgttat cacttattat ggttacaccg gtgtattctt
     3541 caataccttc gatcaggtaa gtgaccgctc ttgaagcgac gaaatatgtc agaatggcgt
     3601 ataaggcgat gtctatcgac aataaataag ctgcagttaa aaatataatt atgttgaaaa
     3661 ttagaataat gtctccgatt gataacattg attttctgct caaatagatt gcaaggactt
     3721 ccgtcccatc caatactgac ccacctcgca tggcaaagcc aatgcctgct cctagaaata
     3781 atccaccaaa actcgcaatt aacaatggat cgtctgtcag tattggaaag tggacaaatt
     3841 ggatgacgat tgcaagtccg gttatggcga tgatgctttt cagggcaaat tctttgctca
     3901 cctgtctgaa accgagtagg atgaaaggcg tattgtacac tattaataaa acagacaggg
     3961 gtaatttgtt gtaaatcact ttggtgagaa ggctgatacc tgttattcca ccatcaatgt
     4021 atccattggg aactaaaaaa ctttctagtc cgaaaccggc cgaaaatata ccaaggatta
     4081 tcaaaagtgc tgacctgatt tggctcttaa ttatagctga tttgggacga acttgcgagg
     4141 gtgccatatt taaattttaa attaccgatt gggtaaaaat ataatttgtt attttattct
     4201 caaaatattt taatcattat taaattttat tgtttatgaa taccctaatt aatgggggtt
     4261 ggtaattcgt aaaaaaaagg ttagacattg actgtaaatc aataatatac atagtataat
     4321 ttaaagattg tgatttgttt ttatctctac cagtcctttt cttgattctc ttatttctaa
     4381 tctcttccca aataattgtt cagccctttg gctctttctc tctatacagg caaatcaatc
     4441 aggctattgg tcctcttata atccggatag ctttgaggtc ctctggtttt tgaattttgt
     4501 tctaacaaaa accaaagtca aaccctcagg cttttatata attgggttcc tttattatat
     4561 attaggcaac ggttaggata aatgatgttc tgagatcttc tggttaacat ccgcatctgt
     4621 tgttgatctg aggaatttat actttttact tctttaaata tttttatcga ctgcgtgata
     4681 tccagttttt taattcttat ttaggaaata gtttgccttg aagtattcaa aagggttaaa
     4741 ggggaagtgg tgccatcctt taagatatgg atcgatgata gtattcactt ggtttgggcg
     4801 gttatttcgg ataagttaac cttgacaacc gttggtcagt cactcacatt aattcctgaa
     4861 aacattgctt agattcgttt ccttttttat ttggatatta catgcttcgt tttatcttta
     4921 gctgtaaata catggatatg ggaaaatctt ttgttgcccg tttttttctt cttctcttat
     4981 tatctttctg catatcattg cagggtcaaa tcaattctaa tatgaaaaag caggataaaa
     5041 aggcttcgcc tccggttgca gccaagagac cgatacattt ggaaaagcac ggtgatatac
     5101 gagtcgatag ttattattgg ctcaaggaaa gagaagaccc cgaagtaata aaatatcttg
     5161 aggatgagaa tacctatatg gaacaatccc tcagtcatac caaaggtctt caggacaaat
     5221 tgtttggtga aattacaagt aggatccctc agattgatat gtccgttccc taccttaaaa
     5281 acggatatta ttactacacc agatatgagt ccggtaagga atatcctatt tatgccagaa
     5341 aaattgacac tttggatgcc cgggagcaag ttctgatcaa tgtcaatgaa tatgcccaag
     5401 gttataatta ttataatatc aagcagattg ctgtcgatcc cttcaataaa tatatggcct
     5461 actcagagga tactctttca cgccgaatat ataccatcaa ggtaatggat atggttaccg
     5521 ggaagtttac cggagatatt ctgcatgaaa acagtggtaa tattgtgtgg ttcaacgatg
     5581 gtaaagcctt cctttatgtc gtgaaggatc ctgagactct gcgggaatac aaggtgtaca
     5641 gacatttggt gggtgatgat agtcgtaatg ataagctgct ttatgaggaa aaggatgaaa
     5701 cttttggcgt tggaatcttt aaatcccgaa gtaatgcatt tatttatatt ggtagctaca
     5761 gtactctgac ctctgaattc agatattgcg acgcgaatga ttctgacgca gagtttaagt
     5821 tgttcgagct ccgaaaacgt gatcatttgt attcagttgc ggatgatgga agacactttt
     5881 atattgtttc caatgacaaa gccatcaatt ttaggctttt caagactgat ctcgacaaaa
     5941 cagagaggag taactggctt gagatgattc ctcacagaaa agatgtcctt attgaggatg
     6001 ttgaatgttt caatgacttt atagttgtgg aggaacgaat gaagggaatg atcaggcaca
     6061 gactcatttc cggggaacaa gactgctatc ttgacttcga tgaggatgcc tatatgactt
     6121 ttttagttga taatcatgag ttcaataccc gaaaacttcg atattcctac acctcgttga
     6181 cgactcctct ttctgtattc gattatgatc tggacacaaa agaacatatt ctgttgaagg
     6241 agcaacaggt aaccggtggc tatgataaga gcctctacac atccgagcgg atagaggtca
     6301 ttgcgcgcga tggcacacgg gtacctgtat ccttagttta taaaaaatca ttgaaaaaaa
     6361 agcgtaataa tcctttatta ttatatggct atggttctta tgggataagc attgaccctt
     6421 cgttttcttc tgttaggctt tcgttgcttg acagaggttt tatatatgca atcgctcata
     6481 tcaggggagg gcaggagctg ggcaggcaat ggtatgagga tggcaaactg ctaaagaaga
     6541 ataacacatt caatgacttt gtggattgcg gacgttatct ggtgacaagg ggttatactt
     6601 caccc
//
LOCUS       DAOQQN010000164         6604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_4925_length_6604_cov_6.101390, whole genome shotgun sequence.
ACCESSION   DAOQQN010000164 DAOQQN010000000
VERSION     DAOQQN010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6604
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4925_length_6604_cov_6.101390"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PLG24_07255"
     CDS             complement(<1..525)
                     /locus_tag="PLG24_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ24724.1"
                     /translation="MFFQHIYDKSLAQGSYLIGCQATKEAIVIDAKRDIDTYLDIARQ
                     NNLNITHITETHIHADYLCGSRELAQVTGANMYLSDEGGEGWQYEFSHIGLRDGDIIK
                     VGNLSLRVIHTPGHTPESISFVLTDHPASDEPVMVFTGDFVFVGDIGRPDLLEKAAGL
                     AGTKEVGAHQMYHSL"
     gene            complement(587..1093)
                     /locus_tag="PLG24_07260"
     CDS             complement(587..1093)
                     /locus_tag="PLG24_07260"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HMZ24725.1"
                     /translation="MTKVTEHRGIFAQLCIIINKSKEIMTEEMTYQIDLKWNEGRIGT
                     VSSPNLDQAITCATPPEFNGGVPNIWSPEHFYGAAINSCFMTTFLAIAEMSRVAFESF
                     ECKTIINLKQIERKYMITQAEISPVIKLADPEKDSERISKVLEKTKANCLVTNSMKTE
                     VTIIPTIL"
     assembly_gap    1625..1724
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2608..4074)
                     /locus_tag="PLG24_07265"
     CDS             complement(2608..4074)
                     /locus_tag="PLG24_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ24726.1"
                     /translation="MKSTIISFKIGFLFSILISVGTTHAQNLFFQRVFDETLAQASYV
                     IADKNTGEAIVIDPKRDIDTYVQIAKENNLIINKVTETHIHADYLSGARELAQVTGAE
                     LLLSGEGGADWQYMFAHKPLMDGDVIRVGSLVLEVMHTPGHTPESITFLLMDKTRKMP
                     VKAVTGDFIFVGDVGRPDLLEKAAGYIGTQEKGAKDLFASVQKFLRFHDDLEIWPGHG
                     AGSFCGKSLSNIPQSTLGQERLTNPALQFGRDEAAFVRYILDGQPDPPKYFAMMKHLN
                     KTPRPLMIMVPKHPILTAQEYHKARANNLLVIDARTLNDHLAGYEAGSLQLNGGKSFS
                     TWAGSMIDYSQQLVIIADRGREEELTRTLMRIGMDNIYGFVNDPSILQLQKPDLLSLK
                     EFKSYLGRKDVEIIDVRSETEYKAGHIKGVKHIPYPDLAGYMTRMDKNKEIVVHCQSG
                     VRSVIAYSMLKANGFKKIRNFAGGVNEWTAGNNEVMIP"
     gene            4426..4935
                     /locus_tag="PLG24_07270"
     CDS             4426..4935
                     /locus_tag="PLG24_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HMZ24727.1"
                     /translation="MIKNVLIAGLFALLSVGAASAQRVAVVDIAGLLENMPEYAAAQK
                     QLDDLSATWRAEINAEMDKIKGLYNKYQAELPLLNDDMKRKREDEITNKEKEVRDLQR
                     ARFGTDGALFAKREELVKPIQDKVYKAIDDYAKERGFDLILDKSSAAGVLFVSDGIDK
                     TADIAKRIK"
     gene            5125..5724
                     /locus_tag="PLG24_07275"
     CDS             5125..5724
                     /locus_tag="PLG24_07275"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HMZ24728.1"
                     /translation="MNRILLFCMITLAFGASSAIAQKVAYINSAVLMQDLPEIKQADS
                     NLNAFQTQLQKKGEQMVTALQAKYQEIAKKQNAGEISPKQLEEESAKLKEEEAKITQF
                     EQDMNKQVAEKRQALYQPILDKINGFIDEVAKKLGYDYVLDSSTGILLFAEDKYDITA
                     DVRHKMEESAKTPAKEETVPAPTQETAPKKSTPAKGGKK"
     gene            5711..6538
                     /gene="murI"
                     /locus_tag="PLG24_07280"
     CDS             5711..6538
                     /gene="murI"
                     /locus_tag="PLG24_07280"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HMZ24729.1"
                     /translation="MAKNNHPIGIFDSGYGGLTVFRSIRDFLPQYDYLYLGDNARAPY
                     GNRSFDTVYEYTLQSVEWFFKQGCPLVILACNTASAKALRTIQQNDLAQRYPDKRVLG
                     VIRPTAEVIAGYSRSGKIGILGTIGTINSQSYCIEINHFSPSTTVYQQACPMWVPFIE
                     NGSHIGNEMDYFIESDLKALFEQNAEIDTILLGCTHYPLIIDKIRQYVPPHVTIVSQG
                     NIVASSLVDYLLRHPDMASRLTKNGMTEFCTTDDPVNFATQGRMFLDEEIEAKHVRL"
BASE COUNT         1909 a         1441 c         1349 g         1805 t
ORIGIN      
        1 cagtgaatgg tacatttgat gtgctccaac ttcctttgtt ccggccaatc cggctgcctt
       61 ctccagcaaa tcaggccgtc cgatatcgcc aacaaagacg aagtcacctg taaacaccat
      121 gactggctca tccgatgcag ggtggtcggt cagcacaaag ctgatacttt caggtgtatg
      181 tcccggtgta tgaatcactc tcagggacag gttgcccacc ttgataatat cgccatcacg
      241 aaggccgatg tgtgagaact catattgcca gccttctcct ccttcgtcag agagatacat
      301 attggcacct gtcacctgag ccagttcacg ggaaccacac agataatcgg catgaatatg
      361 ggtttcggtg atatgtgtga tgttgagatt gttttgccgg gcgatgtcga ggtaggtgtc
      421 gatatctctt ttggcatcga tgacgatggc ttctttcgtt gcttgacaac caatcagata
      481 actgccttgg gccaaacttt tatcataaat gtgttgaaaa aacatgtctt tattctgaat
      541 taatagttaa taaatgtgga aaatacaaac gagccaaatc tcgatatcag agaattgtcg
      601 gaatgattgt cacttcagtc ttcatagagt tagtcactaa gcaattggcc ttagtctttt
      661 ccagcacctt ggatatacgt tcgctgtcct tttcggggtc ggcaagttta attaccggag
      721 aaatctccgc ttgggtgatc atatatttcc gctcaatctg tttgaggtta ataatggttt
      781 tacattcaaa agattcgaag gcaactctag acatttcggc gatggcaaga aaggtcgtca
      841 tgaagcaact attgatggca gctccgtaaa agtgttccgg ggaccaaata tttggcacgc
      901 cgccattgaa ttccggtggg gttgcgcagg taatggcctg atccaggttt ggtgaagata
      961 ctgttcctat tctaccttcg ttccatttga ggtcaatctg atatgtcatt tcttctgtca
     1021 tgatttcctt ggatttatta attatgatgc aaagttgagc aaagattcct ctgtgttctg
     1081 ttacttttgt cacataacta tttttaggcc gaaaaatatt gagattatgc agttcctgat
     1141 tttcagaaag atggtttggt ggccaaaggt gaagaaaaat cgaagtatgt acttaattaa
     1201 tagccgaaaa accgcgatac ttcctgcatt gccaagttca gtttaataaa agtgattact
     1261 tccatggctg gttaataaaa caagtcgtct gtgtagacca tgtgcactca agcagggact
     1321 attaaacatc tacctacttc gctacgaacc accataacag ccgattccgg ccttacaccc
     1381 ggaaaaatcg aagtatgtac ttaattaata gccgaaaaac cgcgatactt cctgtattgc
     1441 caagttcagt ttaataaaaa tgtttacttc catggctggt taataaagca aatctccgat
     1501 gtagaccatg tgcactcaag aagggactat taaacatcta cctacttcgc taagaaccac
     1561 cataacagcc gattccggcc ttacacccgg aaaaatcgaa gtatgtactt aattaatagc
     1621 cgaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgattc cggccttaca
     1741 cccggaaaaa tcgaagtatg tacttaatta atagccgaaa aactgtgaca cttcctgcat
     1801 taccaagatc aggttaataa aaatgtttac ttccatggcg gattaataaa gcaaatctcc
     1861 cgtgtacacc atgtgcactc aagaagggac tattaaacat ctacctactt cgctgcgaac
     1921 caccagaaca gccgattccg gccttacacc cggaaaaatc gaagtatgta cttaattaat
     1981 agccgaaaaa ccgcgatact tcctgtattg ccaagttcag tttaataaaa atgtttactt
     2041 ccatggctgg ttaataaagc aaatctccgg tgtacaccat gtgcactcaa gcaaaggcta
     2101 ttaagcatct acctacttcg ctgcgaacca ccctggcagc cgattccaac caaatgcaat
     2161 tttattaata tcaaaacaat tcaactgaat acaatctcgg cttctgatat actcctcact
     2221 caaatatatg ctggtggtgg ttaagacaca atgatctttc tctgttgatg tctttgttct
     2281 tgagtgtggc accgactcaa cagttcaagg ctgcttcatc tttgataaac atatttatac
     2341 aactactgaa tatcagccaa tacgctgatt cttgaagcac cctgtatgag aaagaggtat
     2401 tgagaaacaa attaaccctg ataatgggtt ttgcatctga tttacccgat gtaagtcaga
     2461 tccatttatg tttaataatg tcagaaattt tgtagctttt tcaccaatca atgaaaacaa
     2521 caagcccccg gattaaaccg gaggcttatt atcttgactt atttgaaacg ctgttgttta
     2581 ctgaaccggg aatctattgg tttgggatca agggatcatt acttcattat ttccggcagt
     2641 ccactcattc acccctccgg caaaatttct aatcttctta aaaccattgg ccttgagcat
     2701 tgaataggca ataacagacc taacgccact ctggcaatga acgacaattt ccttgttttt
     2761 atccatacgg gtcatataac ctgcaagatc aggatagggt atgtgtttca cccctttgat
     2821 atgtccggct ttatattcag tttcactacg tacgtcgatg atttcaacat ccttgcgtcc
     2881 caggtatgat ttgaactcct tcaggctcaa caggtcaggt ttttgcagtt gaagtatcga
     2941 gggatcgttg acaaaaccat agatattatc cattcctata cgcatcaggg tacgggtcag
     3001 ttcttcctct cttcccctgt cggctataat gaccagttgt tggctataat caatcattga
     3061 acccgcccag gttgaaaatg atttgccgcc attgagctga agactaccgg cttcatatcc
     3121 ggccaggtgg tcattaagtg ttcgtgcgtc aatgacaagc aggttatttg ctcgtgcttt
     3181 atgatattcc tgtgctgtca gaattggatg cttgggaacc attatcatca aaggcctggg
     3241 tgtcttgtta agatgtttca tcattgcaaa atatttagga ggatcgggtt ggccatcgag
     3301 gatatatctg acgaatgccg cttcatctct tccaaattga agggccggat tggtcaatcg
     3361 ttcctgcccc aatgtggatt gcggtatgtt gctaaggctt ttaccacaaa atgagccggc
     3421 accatgtccg ggccatatct caagatcgtc atgaaaacga aggaatttct ggacggaagc
     3481 gaacaagtcc tttgctcctt tctcttgtgt gcctatatat ccggctgctt tttcaagcaa
     3541 gtcaggcctc ccgacatcgc ctacaaaaat aaagtctcct gtaaccgcct taacaggcat
     3601 tttccgggtc ttgtccatta agagaaatgt tatactctcc ggagtgtgcc cgggcgtatg
     3661 catcacctcc agcaccagac ttccaacacg gatcacgtca ccatccatca acggcttatg
     3721 tgcaaacata tattgccagt cggcaccgcc ttcaccggat aacaacagtt cagcacctgt
     3781 cacctgggcc agttcacgcg caccactcaa atagtctgca tggatgtgtg tctccgtgac
     3841 cttgttaatt atcagattgt tttctttggc tatctgcaca taggtatcga tgtccctttt
     3901 ggggtcaatg actatggctt cacccgtatt tttatcggct ataacatagc ttgcctgtgc
     3961 cagtgtctca tcaaagactc gctggaaaaa taaattctgg gcatgggtgg ttccgacaga
     4021 tatcaaaatt gaaaacaaaa atccgatctt aaaacttatt attgtcgatt tcatcattaa
     4081 ggaatttatt taaaagaatt ttatttcgaa atgccggtgc aaagaaacaa ctaatccact
     4141 ccccctaatg ttacttttgt cacacaactc tttctcccct ttcagagtat atatgtgcgc
     4201 ttgctttcaa caaataaagt tgatgacatt ggcattgaaa tatccgcaga tatggttaaa
     4261 attgagttaa aacgaaatcc caaacaatga tttgcaatga aacgaagttg atagaatgaa
     4321 aattgatgaa tttaggccaa ttttgtaaaa attggttaaa attacatgag taaccaaaag
     4381 agcacttatt tttgaacaat ttttcaataa aaccaaatta aaaagatgat taagaatgta
     4441 ttgatagccg gcttatttgc cttattgagt gtcggggcgg cctcggcgca gcgtgtcgct
     4501 gtagttgata ttgccggact tttggaaaac atgcctgaat atgcagctgc acagaagcaa
     4561 ttggacgacc tgagtgccac ctggcgtgcc gaaatcaatg ccgaaatgga caagatcaag
     4621 ggtctttaca ataaatatca ggcagaactt cccttgctca atgacgacat gaagcgcaaa
     4681 cgcgaagatg agatcaccaa taaggaaaaa gaagttcgcg atctgcagag agcaagattc
     4741 ggcacagatg gtgcactttt tgcaaaacgg gaagaactgg taaagcccat tcaagataag
     4801 gtctataagg cgatcgatga ttatgccaag gaacgaggct ttgacctgat cctggacaaa
     4861 agtagtgctg ccggtgtgtt gttcgtctct gacggcatag acaagactgc tgatatcgca
     4921 aagagaatca agtaaagggc tgacggcctt taggcaaatt agaattcccg actgaaaata
     4981 agaacggttc ttcaccagtc agacaactct gttatcccgg tgtagtcatc ataatcgaac
     5041 catttgtgat gaagggatga taatttggca aaatgaaagc ttttgattac ttttgcacac
     5101 ttttttaaac caacaagaac aaaaatgaac cgaatattgc ttttctgtat gatcaccctt
     5161 gcttttggag catcaagtgc tatagcccag aaagttgctt atatcaacag tgctgtgcta
     5221 atgcaagacc tgccggaaat caaacaggcc gactccaacc tgaatgcatt ccagacacaa
     5281 ctccagaaaa agggcgaaca aatggtaact gcccttcaag ccaaatacca ggagatagcc
     5341 aaaaagcaaa acgccggcga aatctctcct aagcaactgg aagaagaatc agccaagttg
     5401 aaggaggaag aagccaaaat tactcaattt gaacaggata tgaacaagca ggtagctgag
     5461 aaacgtcagg cactctacca gcctatcctg gacaaaatca atggctttat cgatgaggtg
     5521 gccaaaaaac ttggctacga ttatgtgttg gacagtagta caggtattct tctttttgct
     5581 gaagataaat atgacatcac agcagatgta aggcataaaa tggaagagtc tgcaaagact
     5641 cctgctaagg aggaaaccgt gccggcgcca actcaggaaa ccgcgccaaa aaaatccaca
     5701 cctgccaagg gtggcaaaaa ataaccatcc gataggcatc tttgattccg gttatggcgg
     5761 cctgaccgtc ttccggtcga tcagggattt tttgccacaa tacgattatt tatatttggg
     5821 cgacaatgcc cgcgcgccct atggcaaccg ttcattcgac actgtgtatg agtatacgct
     5881 acagagtgtc gaatggtttt ttaagcaagg ttgcccgctt gtcatccttg cttgcaacac
     5941 ggcgtctgct aaggcattac gcacaatcca acaaaacgac ctggcacaac gctatcccga
     6001 taagcgagta ttaggcgtta tccggcctac tgcagaagtc attgcaggat attccagatc
     6061 cggcaaaatc ggtatccttg gaacaatcgg aacgatcaat agtcaatcct actgtattga
     6121 gatcaatcac ttttcccctt cgacgacagt ttatcagcag gcctgcccca tgtgggttcc
     6181 cttcatcgag aacggcagcc atatcggtaa tgagatggat tacttcatag aatcagatct
     6241 aaaggctctt tttgaacaaa acgccgaaat agacaccatc ctgcttggtt gtacccacta
     6301 cccgttgatc atcgataaga tacgtcaata tgtcccgccc cacgtcacca tcgtatctca
     6361 gggcaatatc gtggcgtcca gccttgtcga ttatctgttg aggcatcccg atatggcaag
     6421 ccgattaacc aaaaatggta tgacagaatt ttgtacaaca gatgatccgg tcaatttcgc
     6481 tacacaggga cgtatgttct tagatgagga aattgaagca aaacatgtga gactttaagg
     6541 agataatgaa tttttttcga aggggccgac tatttgatgg cacaattgaa atactaacat
     6601 ttcg
//
LOCUS       DAOQQN010000165         6529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5011_length_6529_cov_6.048347, whole genome shotgun sequence.
ACCESSION   DAOQQN010000165 DAOQQN010000000
VERSION     DAOQQN010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6529
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5011_length_6529_cov_6.048347"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PLG24_07285"
     CDS             <1..568
                     /locus_tag="PLG24_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HMZ24730.1"
                     /translation="EIGDMSLEAQAKVLRALQEKRITRVGGEKDICVDVRVIAATNKD
                     LLKEIEAKRFREDLYHRLAVIIVHVPSLSDRIEDIKLLSEHFINNICADYGIPPKAIT
                     NDAFKVLEKYNWTGNIRELHNVIERLIILSGDKITADDVINFVQNDPHRKSRKVTDIF
                     EQFSTIDEVQKFIEVAFTDYKSGKGVKK"
     gene            909..1700
                     /locus_tag="PLG24_07290"
     CDS             909..1700
                     /locus_tag="PLG24_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24731.1"
                     /translation="MNFNNIVLKTQPTSLLLLSLAFIAIFSACEKELDTGASEKYKEQ
                     LKESYRYYPLELNKSWIYSIDSIYYSDNLGIIEVDTVRGIYRETLVDTFRNENDQLVY
                     RCLKEKKNVNQTWSVLRVYSLTVLTNQLLRTEDNVTLVDLIFPIRSGSRWDTRVFIDS
                     DANYLIRGKAINLFKDWPDTYVANEYNGEYNGTATPMIDIIDVEADNNIILYKSSHRT
                     YARGVGMIRKEQAFFTTQRTEDPDLPWEQKAEIGFKAIQTLLEYK"
     gene            1700..2239
                     /locus_tag="PLG24_07295"
     CDS             1700..2239
                     /locus_tag="PLG24_07295"
                     /inference="COORDINATES: protein motif:HMM:NF013907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24732.1"
                     /translation="MSRQHLKEYTEVGKVRKSYGLEGVLRAVLYPATVKAAVEAEYFF
                     VEEYGQKLPLFIQWMEGEGDDFHIKFEDIDDPQTAGQLATAPLFMDKRIVEELLPPDE
                     DTLPFDQKDLIGYRIKDLTSGYLIDIQDISQYPEQLMAHIVINNREEMVPLVDDLIEG
                     IDHQQRLITVRFPEGMFGL"
     gene            complement(2363..3016)
                     /locus_tag="PLG24_07300"
     CDS             complement(2363..3016)
                     /locus_tag="PLG24_07300"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HMZ24733.1"
                     /translation="MYLVKTPSFVKRFIPLALWGIRPGEKKIFLTFDDGPIPEVTPWV
                     LDRLDEFDAKATFFCVGENIHKHPEVFNDVIERGHGVGSHTYNHLNGWETDNLEYLLN
                     VKKAAQMSHSVLFRPPYGKLKPKQAQFLHKHYQIVMWDVLSGDFDQNISKEQVYKNVV
                     NNAVDGSIIVFHDSLKAKERMEYALPRVLEYFSEKGFSFAAIQENEAYSPALVPQYA"
     gene            3439..3849
                     /locus_tag="PLG24_07305"
     CDS             3439..3849
                     /locus_tag="PLG24_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HMZ24734.1"
                     /translation="MKDFLDLLGRIFIATIFIYEAYDSIYYFHMTKATMSMYGLTWRQ
                     DLLLVGAIILLVLGGLMVLIGYRSSFGAILLILYYLPVTLIVHSFWNDPVKVQRIQAI
                     MFMKNIAIMGGLLMIIVNGSGRYSIKRLLATLKV"
     gene            3868..4755
                     /locus_tag="PLG24_07310"
     CDS             3868..4755
                     /locus_tag="PLG24_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HMZ24735.1"
                     /translation="MKTDFKKIFLYLMGVALISIASITLHDAFEQEPLPQAIKSPSIN
                     RPFDFAGELVPVANQDVRDRLDKELVMNTYLHGSTLLNLKMAGRYFPLIERILMEENI
                     PDDFKYLVPVESNFSNATSPAGAKGFWQLMPSTAKELGLIVNDKIDERYNVELATRAA
                     CKFIKTLYNRFGNWTLVAAAYNGGGGRIDRELKAQKVNNFYDLYLNPETSKYIFRILA
                     IKEVMSSPSNFGYDIDQVELYKPLTTTRQIEVTGAIPDLTAFAIENGSTYRMLRYLNP
                     WIIDRDLPATGKKLIILLP"
     gene            complement(4925..5905)
                     /locus_tag="PLG24_07315"
     CDS             complement(4925..5905)
                     /locus_tag="PLG24_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24736.1"
                     /translation="MEERGFNNTYKAISITIILLLLALSGYLLYDKSQSANTIANQEA
                     MIVEKEKSRADLEKTYYEALANLEQMKGDNEELNGRIDSLKTELKKQRDQIVSLMQTS
                     KDYQAIKGKIASMKAESEEYKKQIEALRSENETLSRANQQLNTEKEILTGEINKERMT
                     NDEISARNASLETEKANIETERARLNKKVDKASVIKISDILVEGYKLSSKGKEVSKRF
                     AKNIDGLKICFKTNQNELVEAGREKFFIRIINPTGETVSVLNAGSGVIKASEDGAEIP
                     YSLLKECNYENADAVACANWQPNFQFIKGSYEIHVYNKGYMAGKSTFTLK"
     gene            6077..>6529
                     /locus_tag="PLG24_07320"
     CDS             6077..>6529
                     /locus_tag="PLG24_07320"
                     /inference="COORDINATES: protein motif:HMM:NF021613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HMZ24737.1"
                     /translation="MSSSVLLFIDIETVSTVGEYLELNPAMQAFWRKKASGWIRQSDE
                     VNDDLYGNLFKDKAAIFAEFGKIICISIGYNTFDSTTKQAAFRVKSFYGHDEKQLLTD
                     FLNLVAKHFHDPKKHTFCGHNIKEFDIPYICRRAMIHQLHLPPTFQLNG"
BASE COUNT         1897 a         1206 c         1324 g         2102 t
ORIGIN      
        1 tgaaatcgga gatatgagcc ttgaagcaca ggccaaggtc ttaagagctc ttcaggaaaa
       61 acgcatcaca agagtaggag gggagaagga tatttgtgtc gatgtgcggg taattgcagc
      121 aaccaacaag gatttattga aggaaattga agcaaagaga tttcgcgagg acctttatca
      181 tcggcttgca gtaataatcg tacacgtacc atccctttca gatcggatag aagatataaa
      241 gttgcttagt gagcacttta tcaacaatat ttgcgctgat tatggtattc cgcccaaagc
      301 aattaccaac gacgctttca aagtacttga aaaatacaat tggacaggca acataaggga
      361 attgcacaac gtgatagagc gactaattat cctgagtggc gataaaataa ccgccgatga
      421 tgtgattaat ttcgttcaaa atgacccgca tcgtaaatca aggaaggtaa ccgacatctt
      481 tgagcagttc tctactattg acgaagtaca gaaatttatt gaagtggcct ttacggatta
      541 taaatcaggt aaaggggtta agaagtaatc tgataaatca ggacaccaga tttttatcct
      601 tgttgacaac tgtaccttta tccggatgtc gacctcatac taccttctcc ggctaaaggc
      661 cgggaaagta tcgcatttac aatggattgg ataggacagg ataatgtaag agttttcaaa
      721 gaagatgggc tttcgtcgta aactattgtt tttagccttt tgttagatct ctataaaatt
      781 ttcgacattg tcttcaatgt tatatacttt tgtggcgaaa tgatttctgt gtttgtgtat
      841 aagctataag cgtctgacga ttcggatatg acttagattt gaatatcgaa cccgtaattt
      901 tgtgtcattt gaattttaat aatattgtat tgaaaaccca acctacatct ttattactgc
      961 ttagtttggc ctttattgcc atattttcgg cttgtgagaa ggaattggat accggagcga
     1021 gtgaaaaata taaagagcaa ttgaaggaat cctatcgata ttatccattg gaattgaata
     1081 agagctggat atacagcatt gactcgatct attattcgga taatcttggg attattgaag
     1141 tcgatacagt ccggggtata tatcgtgaaa ctttagtaga cacgttcaga aatgaaaacg
     1201 accagttggt atatcggtgt ctcaaggaaa agaaaaatgt caatcagacc tggtctgtct
     1261 tgcgtgtcta tagccttacc gtcttaacca atcaactgct caggaccgaa gacaatgtaa
     1321 cccttgttga tcttattttc ccgatccgat caggaagcag atgggacacc cgggtattta
     1381 ttgactccga tgccaactat ctgattcgtg gcaaagctat caatttattc aaggattggc
     1441 ctgataccta tgtcgccaac gaatataacg gagaatataa tggcactgcc acaccgatga
     1501 ttgatattat tgacgtggaa gcggataata atattatctt gtacaaatca tcacatcgta
     1561 cctatgccag gggtgtgggc atgatcagaa aggaacaggc attttttaca acacagcgga
     1621 ctgaagatcc tgatttgcca tgggaacaaa aagccgagat cggttttaaa gcaatccaga
     1681 cactattgga atataagtaa tgtcccgtca acatcttaaa gaatatacgg aagtaggaaa
     1741 ggtaagaaag agttatggcc ttgaaggagt cttgcgcgct gtactctatc ctgcaacggt
     1801 caaagctgct gtggaagctg agtacttctt tgtggaagag tacgggcaga aactgccact
     1861 cttcattcaa tggatggaag gagaaggtga tgattttcat atcaaatttg aagatatcga
     1921 tgatcctcag acagccggtc aattggcgac agctccatta ttcatggata agcgaattgt
     1981 agaggaatta ctgccgccgg atgaagatac acttcctttt gatcaaaagg atctgatagg
     2041 ctatcgtatc aaggatctga catcgggata tcttattgac atccaagaca tttctcaata
     2101 tccggagcag ctgatggctc acattgtaat caacaatcgc gaagaaatgg tgcctttggt
     2161 cgacgatttg attgaaggaa ttgatcatca acaaagactg atcaccgtac ggtttcctga
     2221 aggtatgttt ggcctttgat ttggtcagtg tgaaataaaa aaagccggga tcctatcacg
     2281 gcttttttat tattcagaaa ttgttgagat tatcctgggg aggtattttt caacttaatg
     2341 cccctgtcca atgttgcaat cgtcaggcat attgaggcac aagggccgga ctgtaggctt
     2401 cattttcctg gattgccgca aaactgaatc ctttttcgga aaaatactcc agtacacgag
     2461 gcaatgcata ctccattcgc tcctttgcct tcaaactatc gtgaaagaca ataatggaac
     2521 catcaacagc attattgaca acatttttat aaacctgctc tttcgagata ttttggtcaa
     2581 aatcaccact caacacatcc cacatcacga tttgataatg cttatgcaga aattgtgcct
     2641 gtttaggctt tagctttccg tatggaggcc tgaacaggac cgaatgactc atttgtgctg
     2701 ccttttttac gttcagcaga tattccagat tgtcagtttc ccaaccattc aagtggttgt
     2761 atgtgtgact gcctacaccg tgtccgcgct caataacatc attgaacacc tctggatgtt
     2821 tgtggatatt ttctccaaca cagaagaaag tagccttggc atcaaactca tccagcctat
     2881 ccagcaccca aggagtcact tccggtatcg gtccatcatc aaatgttaaa aatatttttt
     2941 tttctcctgg ccgtattccc cataatgcca aagggataaa tcttttgaca aatgaaggag
     3001 tttttactaa atacataaaa aaatgttcta aaagtggatt aaattgttga taatattatt
     3061 aacgataatt gcaacgatta cgtttgtgag tgttcagaaa aaaaatcaca gattatcagt
     3121 aattaatata tagcaaattc tatgccgatt ttaattattt gttaaataat ttgagcggca
     3181 atttagcgta tattattgaa atacaatata gtatgctata caaatatttt taataaaatt
     3241 tgctcatttt ttgatctcag ttaaagctaa ttcgtaaaaa catgatattc aatagtttgt
     3301 gttgttgtgt gaaagattgt tatttcctga ggaaggatgc tgtttaagga taaaaatgta
     3361 tttttgtaat taggcaggat tcagaggatt ttgcttaatt gaaatggtga tttttatcat
     3421 tagcctaatt caatcacaat gaaggatttt ctggacctgc tgggccgtat tttcatagcg
     3481 actattttta tttatgaagc ttatgactcc atatattatt ttcatatgac aaaagcaacc
     3541 atgtcaatgt atggtctgac atggagacaa gatctcttgc tcgtaggtgc cattatattg
     3601 cttgtgttgg gtggtttgat ggtgttgata ggttacagat catcttttgg tgcaatcctt
     3661 ttgattttgt attatctgcc ggttactttg attgtccatt ctttttggaa tgatccggtg
     3721 aaggttcagc gtattcaggc tattatgttt atgaagaata ttgctatcat gggcggcttg
     3781 ttgatgataa tagtcaatgg ttcaggtcgt tatagtatca aaagattatt agctactttg
     3841 aaggtttgat tttttaatat ctcaggaatg aaaacagatt ttaaaaagat tttcttatat
     3901 cttatgggtg ttgctttgat ttcgattgca tccataacac ttcatgatgc ctttgaacag
     3961 gagccactac cccaggctat taaatctcca tccatcaatc gaccttttga ttttgccgga
     4021 gaacttgttc ctgttgccaa tcaggatgta agggatcgac ttgataagga gctggtaatg
     4081 aatacttacc tccatggctc caccttgctc aacctcaaga tggcaggaag gtattttcca
     4141 ttgatagaaa gaatattgat ggaggagaat attccggatg atttcaaata tcttgttcct
     4201 gtcgaaagta atttcagtaa tgccacttcc cctgcaggtg caaaaggttt ttggcaattg
     4261 atgccctcca cggcaaaaga gctgggctta atcgtcaatg ataagataga tgaacgatac
     4321 aacgtagagc ttgcgacccg ggctgcttgc aagtttatca aaaccctgta caacagattt
     4381 ggcaactgga cactggttgc tgctgcatat aatggcggag gtggtaggat tgatagagag
     4441 ttgaaggcac agaaagtcaa taatttctat gatctgtatc tcaatccgga gacctctaaa
     4501 tacatcttcc gtattctggc tattaaagag gtgatgagta gtccttccaa tttcggttat
     4561 gacattgacc aggtagaact ttataaacca ttgactacaa caaggcagat agaagtaacc
     4621 ggggcaatcc ccgacctgac agcatttgcc atcgaaaatg gttcgactta ccggatgctc
     4681 aggtatttaa atccatggat tattgatcgg gatcttccgg caacagggaa gaagttgatc
     4741 attcttcttc cataagtctc ttgcttatca ggataaattt atcccggaaa tgaaccgatc
     4801 acgcttttaa gaatacacta cattcaatca ttgaagtaat tttgggctgc aatgacggga
     4861 agatacaaag catttggatc caatctttat gcctttacgt ccttcaatat tcgcactgaa
     4921 gcatctactt cagagtgaaa gtacttttcc ctgccatata ccctttatta tatacatgaa
     4981 tctcatagga accctttata aactgaaaat tgggttgcca gttggcgcat gctacagcat
     5041 ctgcgttttc gtagttacat tccttgagta gcgaatacgg gatttcggcg ccatcttcgg
     5101 atgccttgat tacccctgag cctgcattta aaacactgac tgtttcgcct gttggattga
     5161 ttatcctaat gaagaatttt tcacgccctg cctccacgag ttcattttgg ttggttttaa
     5221 aacaaatttt caatccatcg atattttttg caaacctttt tgaaacttcc ttccctttgg
     5281 aagacaattt gtaaccttcg acgagaatat cagaaatttt aataacggat gccttatcca
     5341 cttttttatt cagtcttgcc ctttcggttt caatattggc tttttccgtt tcgagggagg
     5401 cattgcgggc cgaaatttca tcatttgtca ttctctcttt gttgatttca ccagtgagaa
     5461 tttccttttc ggtattaagc tgttgattgg ccctggataa ggtttcattt tcacttctga
     5521 gggcttcaat ttgttttttg tattcttcgg attcggcctt catggaagca atttttcctt
     5581 taattgcctg atagtctttg ctggtttgca tcagggagac aatttggtcc cgctgttttt
     5641 tcaactctgt cttgaggcta tctatcctgc cgtttaactc ttcattgtcg cccttcattt
     5701 gctcaaggtt ggccagagct tcataatagg ttttctccag gtctgctctg cttttctcct
     5761 tttcaacaat cattgcttcc tgattggcaa tggtattggc gctttggctt ttgtcataca
     5821 gcaggtagcc actcaaagcc aataatagca atataattgt aatacttata gctttgtaag
     5881 tattgttaaa acctctttct tccattttga tagatttaaa ttaatatgaa aatctttttt
     5941 gatataaata agtttcaaaa tgcaaatgta ttgtattttt aattgataaa attaatagta
     6001 taccactatt atttggatgg cgggaagtca gtattttttc tcctttcttc attaatttaa
     6061 tataattaag gttgttatga gctcatctgt tttgttgttt atcgatatcg agacagtttc
     6121 cacagtagga gagtatcttg aattgaaccc tgccatgcag gcattttggc ggaaaaaggc
     6181 atccgggtgg atcagacaat ccgatgaagt caatgatgac ctatatggta atttgttcaa
     6241 ggataaagca gcaatttttg ctgaattcgg gaaaataatc tgtatttcaa tcggctacaa
     6301 cacttttgat tcaaccacaa aacaagccgc tttcagagtt aaatcctttt atgggcacga
     6361 tgagaaacag ttgttgacgg attttttgaa tctggtagcg aagcattttc acgatccaaa
     6421 gaaacatacc ttttgtgggc acaatatcaa ggaatttgat atcccgtata tatgtaggag
     6481 agcaatgatt catcagttac atcttccgcc aaccttccag ctgaatggg
//
LOCUS       DAOQQN010000166         6518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5022_length_6518_cov_5.362525, whole genome shotgun sequence.
ACCESSION   DAOQQN010000166 DAOQQN010000000
VERSION     DAOQQN010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5022_length_6518_cov_5.362525"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PLG24_07325"
     CDS             <1..671
                     /locus_tag="PLG24_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24738.1"
                     /translation="KQNTAIDFYGCNKLFGIKVLDLADLKKDGRQLYYIEDGKKIGIE
                     KIYNRVIFDELEKRKDIVSEFNFKDEVDVEWIGHPNWFFKISKYTMPFIRNNPYIPET
                     RFLNTIQHIPEDLENFVLKPLFSFSGEGVKFHVTKKDIEGVKDPENWILQEKVNYYPA
                     IKTPNPDEPVKFEIRMMHTWEQGAPAPLLVNNLIRLSKGDMVGVKYNKDKDWIGASIG
                     FFEQ"
     gene            726..1139
                     /locus_tag="PLG24_07330"
     CDS             726..1139
                     /locus_tag="PLG24_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24739.1"
                     /translation="MYDIPIERLIPQRPPFVLVDGIKEHETAVTKAHFLIPKDHILIE
                     HGKLSAYGLLENMAQAAALRSGYESLEKGENPKTGFIGTITNAIVHALPEAGTEVITT
                     VHQTNQILNFILIECTAKSGDLVLAECAMKIVLLD"
     gene            1593..2204
                     /locus_tag="PLG24_07335"
     CDS             1593..2204
                     /locus_tag="PLG24_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ24740.1"
                     /translation="MDTKDLLGYKAFEIFVRYGFRAVTMDDLAKEMSISKKTLYQHFN
                     NKEDVIRQALNVYHKRQKTKMENIQKEAQNPIEVIFLVFKILIDESKDVNIMNFLDLK
                     KYYFDIWQNSANSNEEYFCNRISTNLERGMAIGFYRPDLNRDIIAKLFYHLALALTSQ
                     DNNLLNEYPLRDFIRHIVTYHLNGVCTDEGRTILENYIKQYFD"
     gene            2226..3557
                     /locus_tag="PLG24_07340"
     CDS             2226..3557
                     /locus_tag="PLG24_07340"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ24741.1"
                     /translation="MKRLFTYLLLIACWTSSLPISAQQSFSLNQAVEYGIKNAAATKK
                     DLLKIADAEQQIKAVRASGFPQFNGSVEYQYFAEVPEMVIPKEFDPTGQGGTVSFQKS
                     NNLNLGINTGFVLFDGAYLVGLKAAKFYRELVHAQAEITPINIRKNITDSYYTVLIAM
                     KNKEQLEKNITVLQKMRGEAEQIYKNGFAEKMDIDRLDLSISNLQSTMNNLMRNIEIL
                     KNILKFQMNYPLEQEITLTDEFDVLFLQASKDDGKSIEQFNINKRPEYRAMGRGIELA
                     ALDVKRQQQGYLPSLSFYASFTRGLQSDNIFKPGGFWIPTTVIGLKLNVPIYDSGMKK
                     AVIQRARIVVEQNTLDRSEFERAGNIEVTNTQKAYLNSIETVKEKQKSLDLAEEIFKV
                     AQTKYKEGVGTSLEISQAETDLYGAQAALIQAQGDVINARFAVEKALGNFN"
     gene            3585..4736
                     /locus_tag="PLG24_07345"
     CDS             3585..4736
                     /locus_tag="PLG24_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ24742.1"
                     /translation="MKNNILLSMIILTSLSLVSCSNEGNSSKSLAAKQKALDKKIALR
                     SQLDKEIEALEKEIAALDTTSIKEVTKIVGITNINTGSFSSQVEVMGKVDVANNASLS
                     AAQGGIVTHIFVKEGDHVKTGQTLAQLDNDVMNKSLIQARQAVAFTTDLFNKQKALWD
                     QKIGSEVQYLNAKNNMESAQNQLNIALQQNELFKIKAIYSGVVDLVDIKKGQVVAPGM
                     PAFRIVATKGLKLIANVPESYIGKIKQGNTVQVYFPDLNKEITGKVDYLSRVIDPLSR
                     TINAEILLPDNQSEIKTNMVGILRIKDYNNANAITIPVKNLLKNAEGYYVFIAEKSGE
                     KLVAKPRQIKTGAANGDKIEVTEGLKAGDQLITTGFQSIHEGDVLIMSE"
     gene            4788..>6518
                     /locus_tag="PLG24_07350"
     CDS             4788..>6518
                     /locus_tag="PLG24_07350"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMZ24743.1"
                     /translation="MQDINKEFKPTTWSIKNKTSIYLATLFITILGIATYFSLPKENF
                     PEVVIPQIFVATVYAGTAPADMENVVTKVLEKELKAISGVKKVTSNSFQDYSTIIVEF
                     NTGIKIDVAKQKVKDAVDKAKPDLPNDLTKEPDVIDINLSDLPIMNINLSGDMDLLQL
                     KEYAEDVKDRLESIKEINRIEIVGALEREIQVNVDLFKMAAAGASFMDIENAIKYENM
                     TISGGEINMDGTKWAVSVKGEYRNPLLMNDLMVRTSSGGTIYLRDVATVLDATAEQES
                     YARLDGKNVITLNVIKRAGENLISASHSIKQELENMRGRVLPANIKATITQDQSIETE
                     ITLHDLINTIILGFLFVTFVLMFFMGTSNALFVGLSVPISAFLTFLVLQGAGYTMNMI
                     VLFSFLLGLGIVVDDAVVVIENTHRIFANGKVNIFKAAKMAAGEVFLPVLTGTVVTLT
                     PFIPLLFWPGIIGDFMYYLPFTLIIMLLASLLVAYLINPVFAVDFMKPHKSHKDTIAL
                     DKSFIVTSAAFVAAAIFGYLMNVGFGNLVVTVYVFFVLNKFVFSWLIDKFQTNVWPKF
                     QNVYYNIVKSS"
BASE COUNT         2165 a         1310 c         1337 g         1706 t
ORIGIN      
        1 aaaaacaaaa tactgcgata gatttctacg gatgtaacaa actgtttggg atcaaagtac
       61 tcgacctggc agacttaaaa aaagatggca ggcaacttta ttacatcgag gatgggaaga
      121 aaatcggtat tgagaaaata tacaatcggg ttatcttcga cgaactggag aaaagaaaag
      181 acatcgtttc agaatttaat ttcaaggatg aagtggatgt ggaatggatc ggccatccca
      241 actggttttt caagatcagt aagtacacca tgcctttcat aagaaataat ccttatattc
      301 ctgaaaccag atttctgaat accatccaac acatcccgga agatcttgaa aattttgttc
      361 ttaaaccatt gttttccttc agcggcgaag gggtcaagtt tcatgtaacc aaaaaagata
      421 ttgaaggtgt aaaagatcct gaaaattgga ttcttcagga aaaagtaaat tattaccctg
      481 caataaagac cccgaatccg gacgaaccgg tgaagtttga aatccgtatg atgcatactt
      541 gggaacaggg agcccctgcc ccacttttgg tcaacaacct gatacgattg agcaaaggtg
      601 atatggtagg agtcaaatac aacaaagaca aggattggat aggcgccagc atcggcttct
      661 ttgagcaata gcttacatgc caggctgagc agattgaata gtagctacta caaattaaca
      721 atgaaatgta tgatattcca atagaaagat tgattcctca aaggcctccc tttgtattag
      781 tggatggaat aaaagagcat gaaacagcgg ttacgaaagc acactttctg atccccaaag
      841 atcatatttt gattgagcat gggaaacttt ctgcatacgg tctcctggaa aacatggcac
      901 aagcggcagc actccggtcc ggctacgaat cattggaaaa gggagaaaac ccaaaaaccg
      961 gatttatcgg caccataacc aatgcaatag tacatgctct gcctgaagcc ggtactgaag
     1021 taattacaac cgtccatcaa accaatcaaa tcctgaactt catcctgatt gagtgcaccg
     1081 caaaatccgg agatcttgta ttggctgaat gtgccatgaa aattgtcctt ttggattgaa
     1141 cttttgcttc cttgcccgat tttaaataag ctcttccctg ctgaaattat acttttagca
     1201 caatacaata acttaataaa gctattctgt aagaacggtt gacaaagtac gacctcttta
     1261 atcaacaaaa atgacattgt caagataata cgcgtgtgat atttttccct tttatttgaa
     1321 aaaataatta ataatcatca gtcagtattg aagattaatc tgaatattta tttatttcat
     1381 cgaaaagcat aacactacag aacgaaatat ataccaatta tttattttta cataaatatt
     1441 ttattctgta tttctaatat ttttgttata tgattttata aattgatgaa tatttagttt
     1501 tttatacaca ggaaacgttt tttgtttttt tgtttccatt ttatcaaaat agtttatttt
     1561 tgcacatcaa aagtagagtt ttaagattca caatggatac aaaggactta ctaggctata
     1621 aagcatttga aatttttgtc aggtacggat ttcgggcggt cacaatggat gacctcgcta
     1681 aagaaatgtc tatctcaaaa aaaacgctat atcagcattt caacaacaag gaagatgtga
     1741 tcaggcaagc actgaatgtt tatcacaaac gccagaagac caaaatggaa aatattcaga
     1801 aagaagcgca aaatcctatt gaagtaatct ttctggtttt taaaattctc attgacgaat
     1861 caaaggatgt caatattatg aatttcctgg atctcaaaaa atactatttt gatatctggc
     1921 aaaattctgc aaatagcaat gaagagtatt tctgtaacag aataagcaca aatcttgagc
     1981 gcggtatggc cataggcttt tacagacctg atctgaaccg ggacatcata gcaaagctat
     2041 tctatcatct ggcactggcg ctgaccagcc aggacaacaa ccttctcaac gaatatcctt
     2101 tgcgggattt tattcgacat atcgtaacct accatctcaa tggtgtatgt accgatgaag
     2161 ggaggacaat tttggaaaat tacataaagc aatattttga ttaacaacat aatacatcag
     2221 taaatatgaa gcgattgttc acatatctgt tactcatcgc atgctggaca tccagcctgc
     2281 ccatctctgc ccagcaatca tttagcctca atcaggcggt ggaatatggt ataaagaatg
     2341 ccgcagcaac taaaaaggat ttactgaaaa tagctgacgc agaacaacag atcaaggcag
     2401 tgagagcttc cggcttcccg caatttaatg gttctgtgga ataccaatat tttgcagaag
     2461 ttccggaaat ggtcatacca aaggaatttg accctacagg acagggcggt actgtatcat
     2521 tccagaaaag caacaatctt aatctgggca tcaataccgg ctttgtactt ttcgatggcg
     2581 cctacctggt tgggctgaaa gctgccaaat tttacagaga gttggtccat gctcaggccg
     2641 agattacacc catcaatatt cgtaaaaata ttacagactc ctattatact gttctgatcg
     2701 ccatgaaaaa caaagaacag ctggaaaaaa acattaccgt gttacagaaa atgcgtggcg
     2761 aagcagaaca aatttataaa aacggttttg cagaaaaaat ggatatcgac aggctggacc
     2821 tgagcataag caaccttcag tcaaccatga acaacctcat gcgaaatatt gaaatcctga
     2881 aaaatatcct gaaatttcag atgaattatc cactggaaca ggaaatcaca ttgacggatg
     2941 agtttgatgt attattcctt caggcgagta aggatgatgg aaaatccatt gaacaattca
     3001 acatcaacaa acggccggaa tacagagcaa tgggcagagg aatagaatta gcggctcttg
     3061 atgtaaaaag acaacaacaa ggataccttc ccagcctttc gttttatgcc tcatttaccc
     3121 gaggattgca atccgacaat atattcaaac cgggcggatt ttggatccct accacagtca
     3181 taggactcaa actcaatgtg cctatatatg acagtgggat gaagaaggct gtcatccaac
     3241 gagctagaat cgtggttgaa caaaacaccc tggatcgctc agaatttgaa cgtgccggaa
     3301 atattgaggt taccaataca caaaaggctt atttaaattc catcgaaacc gtcaaggaga
     3361 aacaaaaatc tctagatctc gcggaagaaa ttttcaaggt cgcccaaaca aaatacaagg
     3421 aaggagtagg tacgagtttg gaaatctcac aggctgaaac ggatctctac ggcgcacagg
     3481 ctgcattgat tcaggctcag ggcgatgtca tcaatgcaag atttgcagtg gaaaaagccc
     3541 tgggtaactt taactaattc aaaataaaga aaaaacgata taacatgaaa aataatatcc
     3601 tgctttcaat gatcatcttg acatcactat ccctggtgtc ctgcagcaac gaaggaaaca
     3661 gtagtaagtc cctggctgca aagcaaaagg cactggacaa gaaaatcgct ctcagaagtc
     3721 aactggataa ggagatagaa gccctggaaa aagaaatagc ggccctggac accacttcga
     3781 taaaggaagt tacaaaaatc gtaggaatta caaatataaa tacaggaagc ttttcctctc
     3841 aggtagaagt catgggtaag gtagatgtgg ccaacaatgc aagcctgtca gccgctcagg
     3901 gaggtatcgt cacccacata tttgttaagg aaggagatca tgttaagaca ggtcagaccc
     3961 tggcccagct tgataatgat gtcatgaaca aaagcctgat ccaagcgcga caggcagtag
     4021 cttttacaac cgaccttttt aataagcaaa aggcattgtg ggaccaaaag ataggcagcg
     4081 aagtgcaata cctcaatgcc aaaaacaata tggaaagcgc gcaaaatcaa ctcaatattg
     4141 cactacaaca gaatgaactt ttcaagatta aggcaatata ctcaggcgtg gtggacttag
     4201 tcgatattaa gaaaggccag gtggtagctc ctggaatgcc cgctttccgt attgtcgcaa
     4261 ccaaaggtct caaactgatt gccaatgtcc ctgaatcgta tattggcaag ataaaacaag
     4321 gcaatactgt ccaggtttac ttcccggatc ttaataaaga aataaccgga aaagttgact
     4381 atctaagcag ggtcatcgat ccattgagca ggaccatcaa cgcggaaatc ctcttaccgg
     4441 ataatcagtc ggagataaag accaatatgg tggggatact cagaatcaaa gactacaata
     4501 atgccaatgc aattacgata ccggtaaaga acctgttgaa gaatgcagag ggatactatg
     4561 tctttattgc ggaaaaatcc ggagaaaaat tggtcgcaaa accaaggcaa ataaagaccg
     4621 gtgctgccaa tggagataaa attgaagtaa ctgaagggct gaaagccggt gaccagttga
     4681 taacgaccgg attccagagt attcacgaag gcgatgtcct gatcatgagc gaataatccg
     4741 gctccaatca tccggcaaac ttttcaatat catttagaaa tacagatatg caagatataa
     4801 ataaggaatt caaacctacc acctggtcca tcaagaacaa aacgtccatt taccttgcga
     4861 ctttgtttat aaccatcttg ggaatagcaa cctacttcag ccttcccaag gaaaatttcc
     4921 ccgaagttgt cattccccag atattcgttg ccactgtcta tgcaggtaca gctccggctg
     4981 atatggagaa tgtagtgacg aaagttctcg aaaaagaatt gaaggcaatc agcggagtaa
     5041 agaaggttac atcaaattct tttcaggatt attcaaccat catcgtcgaa ttcaataccg
     5101 gtatcaagat agatgtagcc aaacaaaagg tcaaggatgc tgtagataag gcaaaacctg
     5161 atctgcccaa tgatctgacc aaagaaccgg atgttatcga tatcaacctg tcagacctgc
     5221 ccatcatgaa catcaacctg tcaggagata tggacttgct acaattgaag gagtatgcag
     5281 aagatgtcaa ggacaggtta gaatccatca aggaaatcaa tcggatagaa attgtaggag
     5341 ctcttgaacg ggaaatccag gttaatgtgg atttgtttaa gatggcggct gccggagcat
     5401 ccttcatgga tatagagaat gccatcaagt atgagaatat gaccatctcc ggaggagaaa
     5461 ttaacatgga tggtaccaaa tgggctgtaa gcgtaaaggg agagtatcgc aatcctctgt
     5521 tgatgaacga ccttatggtg aggacaagca gcggaggaac catttatttg cgcgatgtag
     5581 ccactgtgtt ggatgcgaca gccgaacaag aatcatacgc ccgcctggac ggcaaaaacg
     5641 tcattacgct taatgtaata aaaagagccg gtgagaacct tatttccgcc tcccactcca
     5701 ttaagcaaga actggaaaac atgagagggc gggtattgcc ggccaacatc aaggcaacaa
     5761 taacccagga ccagtccatc gaaacagaaa ttaccctgca cgatcttata aatacaatca
     5821 ttctgggctt tctcttcgtg acctttgtct taatgttttt catgggaacc agcaatgccc
     5881 tttttgtagg acttagtgtc cccatttctg cattccttac cttccttgta cttcagggag
     5941 caggatatac catgaacatg attgttttgt tttcattcct gcttgggctc ggaattgttg
     6001 ttgatgatgc cgttgttgtg atcgagaaca cacacaggat ctttgcaaat gggaaggtaa
     6061 atattttcaa agctgctaaa atggctgcgg gagaagtatt cttacctgtt ttgaccggaa
     6121 cagtggttac cctgacccct ttcataccgt tattgttttg gcctggcatc ataggagatt
     6181 ttatgtacta cctgcctttt acgctcatta ttatgcttct ggcatccctt ttggtagcct
     6241 atctcatcaa tccggtattt gccgttgact tcatgaaacc ccacaagagc cacaaggaca
     6301 ctattgcgct tgataaaagt tttatagtca ccagcgcagc ttttgtcgcc gctgcaatat
     6361 tcggatatct catgaatgtc ggttttggca acctggtcgt cacagtctat gtattctttg
     6421 tattgaataa atttgtgttc tcctggctga tcgacaagtt ccagacaaat gtttggccaa
     6481 agtttcaaaa cgtctattat aatatcgtca agagttcc
//
LOCUS       DAOQQN010000167         6517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5023_length_6517_cov_5.474621, whole genome shotgun sequence.
ACCESSION   DAOQQN010000167 DAOQQN010000000
VERSION     DAOQQN010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5023_length_6517_cov_5.474621"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="PLG24_07355"
     CDS             <1..960
                     /locus_tag="PLG24_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMZ24744.1"
                     /translation="FWTCFILGMLCLLHIKSVGQGRVISNFNQNGFSRHFDTSSNALY
                     INHNDKVMSYDSRGLFYFARYLPGDMWKLAKSPFQKENLEGLGLVLISTAALLPFDQY
                     FLDKVRQASRSINLADNTSFGVAVGKGEIILVKYPRNVNSALYQLGEGGTSMYLAGGL
                     WIYGKIARDYRAVQTAGDLVETFIVMGVTTQILKRISGRESPYVSTQPGGAWRPLPSL
                     KEYQSHVPRYDAFPSGHLATMMATVTVLHNNYPEKKWILPVGYSLMGMSAWAMMNTEV
                     HWISDYPLALALGYISGKISTMRHNRSQSEVMKNRVLNPEMTP"
     gene            complement(987..1457)
                     /locus_tag="PLG24_07360"
     CDS             complement(987..1457)
                     /locus_tag="PLG24_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HMZ24745.1"
                     /translation="MIEINNLASQSVRLAVENVLNEGGPFGCIIVRNGEIIATGVNRV
                     TFDNDPTAHAEVMAIRAACTKLHTYQLDDCDLITSCEPCPMCMGAAYWARVKAVYYVA
                     TKADAASAGFDDSFIYEEMEVPYHNRKIPFIRVELNENSAPFEAWLKKENKKLY"
     gene            complement(1507..2346)
                     /locus_tag="PLG24_07365"
     CDS             complement(1507..2346)
                     /locus_tag="PLG24_07365"
                     /inference="COORDINATES: protein motif:HMM:NF025007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HMZ24746.1"
                     /translation="MILEPIERRTNLSREEFKKEYLDPGKPVVIEDLAKDWAGLQKWS
                     FGFFKEKYGDLMVPLVDQSFHDPGARYMEHRNIPFRDYIEAIEKGPTDLRLFLFNIFD
                     KAPELREDFIWPTIMDGFVKSLPFMFFGGAGSITPMHFDIDCPSNFLTHFHTRKKIIL
                     FDRNQSRYLYHQPFTVQSQVDINNPDYDQFPALRKARGIEAILHHGDTVYIPPHWWHY
                     IEYVDSGFSLALRAHDRISNKFVAVQNLIQHSLIDKGMNKILGKKWKNWKVNAARKRA
                     ESA"
     gene            complement(2613..4481)
                     /locus_tag="PLG24_07370"
     CDS             complement(2613..4481)
                     /locus_tag="PLG24_07370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HMZ24747.1"
                     /translation="MKRFLISTFVIIICWVGTNAQDSLDSLWFRNNYTKMEKMIPMRD
                     GTKLFTSIYIPRDLSGPHPIIMRRTPYSCRPYGLDNYAQFYNGYFRNYLRSNYIIVTQ
                     DVRGRFMSEGDFIDIRPLLAERKSDQDIDEATDTYDTVEWLVNNLENNNGKVGIMGIS
                     YPGFYSTVAALSGHPAIKAVSPQAPVTDWFRGDDFHHNGALALIDAFTFYLNFGRPRP
                     KPTMGSGWSNTQFGPDAYRFFLDAGPLSDIKHKYFGDSIQFWNDLFAHPNLDKFWEDR
                     NPLHHIKDVKPAIMVVGGHFDAEDCYGAWETYKSFARKSPQTNTTLVMGPWFHGNWGG
                     RGSGEQFGPIRFGSKTAEWYQNEFEIPFFKKYLEDNPTPTIGDKVNVFITGSNHWAAF
                     NQWPPEGVEEFRLFLSENNNLRNDKSTQSGVYDQYTSDPSHPVPYRPDISWNRNREYM
                     IEDQRFASQRPDVLTYQTDVLTKDVTMLGPVLADLFVKLTTTDADFVVKVIDVFPDDF
                     NEYTSRDVPMGGYQMLVRGEIFRGRYRKSFSHPEGFKPGKIEQVNFSLPDIAHTFKKG
                     HRIMVQIQSSWFPLFDLNPQQMVDIYRCKKKDFISSTIQIIRSKEYPSSVSFYVKK"
     gene            complement(4799..>6517)
                     /gene="sprA"
                     /locus_tag="PLG24_07375"
     CDS             complement(4799..>6517)
                     /gene="sprA"
                     /locus_tag="PLG24_07375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HMZ24748.1"
                     /translation="IRQLNADFSFDRLYSKWNYLKKIEKPESKSTSKISQPKQKGSNT
                     KPVTDDPGNKDALGKQDSTDAKLSKAERLKLRKEKEKEKRKLAREERRKNYQPSLAER
                     ILIRPLLSLRKARLTYTENLNSTIPGYVPSTSSMGMDQWSAPGFGYIAGWQPDDAYFE
                     RAVAPNNNWITKNIFQNQLVILGQNQAIDAKINLEPFNDFKIDLNANRTYQNTHTELF
                     KVKAIGEDFGRYAGRDIGSYTTTYFTLNSFFDDMRSVFKRFESNRTVISNRLGTTPDP
                     NVPGYTTGYGKIQQDVLIPAFLAAYSNTDPTKQNLDIFSTFPKINWQLTYNGLTKLEL
                     FKKYFSSVNLTHAYKSTLNVNSFNTSPNYDPEDPYIFNPITNNYYSRLEIPAVSITEG
                     LSPLIRLDVKTKNDITFNIDYKKVRNLSLSFVDNLVNETKSSDITIGAGYTIHSVKMP
                     FMKEFQLKKNDKKKKDKGKDPSKTSTGPGPKGGNKAAENDLIIRLDVSYRDDATVSHF
                     IDQDLTRTSRGAITLRLAPNIEYQYNKNLTLRLFYDYNLTNPKTSQQFKTISQRGGIT
                     FRFLLN"
BASE COUNT         1721 a         1307 c         1409 g         2080 t
ORIGIN      
        1 ttttggacgt gcttcattct gggaatgctc tgtttgcttc atataaaatc agtgggccag
       61 gggagggtaa tttcaaattt caatcagaat ggattttcca gacattttga tacttcttcg
      121 aatgctttat atatcaatca caatgacaag gtgatgtctt atgattcacg cggtcttttt
      181 tactttgcaa gatatctccc cggcgatatg tggaaactgg ctaaatctcc ttttcaaaaa
      241 gaaaacctgg aaggtcttgg actggttttg atttcgaccg ctgctcttct tccatttgac
      301 caatacttcc ttgacaaggt tcggcaggca tcccggagta tcaacctggc agacaacact
      361 tcctttggtg ttgcagttgg taagggagag attatacttg taaagtatcc tcgaaatgtc
      421 aattcggctt tatatcagct tggagagggt ggtacaagta tgtatctcgc cggggggctg
      481 tggatttatg gtaagatcgc cagggattac agagcggttc agacagcagg tgatttggtg
      541 gaaacattta ttgtcatggg agttacaact cagatcctga aacgaatttc aggaagggaa
      601 agcccctacg tatcgactca accgggagga gcatggaggc ctttgccatc cctgaaagag
      661 tatcaatctc atgtgccaag gtatgatgct ttcccatccg gccatctggc taccatgatg
      721 gctacagtga cggttttaca taacaattat cctgagaaga aatggatttt acccgttgga
      781 tattcattaa tgggtatgtc cgcctgggct atgatgaata cagaggtaca ctggattagt
      841 gattatcctt tagccttggc attgggatat atatccggca aaatttctac catgcgacac
      901 aacaggtcgc aatcggaagt tatgaaaaat cgggttctta atccggagat gacaccttga
      961 tttcaagttc taaaggcaat atattttcaa tagagttttt tattttcctt ctttagccaa
     1021 gcctcgaacg gggcactatt ttcatttagc tccacccgga taaacgggat tttgcgattg
     1081 tgataaggga cctccatttc ttcgtaaatg aaagagtcat caaacccggc agaggcggca
     1141 tctgccttag tagccacgta ataaacagct tttacccttg cccaatatgc agctcccatg
     1201 cacatgggac agggctcaca agaggttatc aggtcgcaat cgtccaactg gtaggtatgt
     1261 aactttgtgc aggctgctct gatagccata acctctgcat gtgcagtagg atcattatcg
     1321 aaggtcaccc tgttgacacc tgttgctatg atttcaccat tcctgacaat aatacaaccg
     1381 aatggtcctc cttcattcaa aacattttca actgctaacc gtaccgattg ggatgccaga
     1441 ttgttgattt cgatcatttt ttattttgtt cttcaattgg ttttggacaa tatgtcctga
     1501 tattaatcaa gcagattccg ctctcttacg tgcagcgttg accttccagt ttttccattt
     1561 tttacccaga attttattca tgcccttgtc gatcaacgaa tgttgaatca aattttgaac
     1621 ggcgacgaat ttattggata tcctgtcatg tgctctcagg gcaagggaaa agccactatc
     1681 tacatattcg atataatgcc accaatgagg gggtatgtac actgtatcac catggtggag
     1741 tatggcttca attccacgtg ccttgcgaag tgccggaaac tgatcataat ccggattgtt
     1801 gatgtccact tgactttgaa cggtaaacgg ctggtggtaa agatacctgg attgatttct
     1861 gtcaaagagg atgatttttt tcctggtatg aaaatgagtc agaaaattag aagggcaatc
     1921 tatatcaaaa tgcatcggcg ttatacttcc tgctcctccg aaaaacataa agggcagtga
     1981 cttgacaaat ccgtccatga tagtaggcca gatgaaatct tctctaagtt ccggagcctt
     2041 gtcgaagata ttgaataaaa aaagacgcag gtctgttggg cctttctcga tggcttcaat
     2101 gtaatccctg aacggtatat tcctgtgttc catgtagcga gcacctgggt catggaagga
     2161 ctggtccact aatgggacca tcaaatctcc atatttttct ttaaaaaacc cgaacgacca
     2221 tttttgcaga cccgcccaat ccttggcaag atcctctatt acaacaggct tccctggatc
     2281 caggtattct ttcttgaatt cttccctgga tagatttgtt cttctttcta ttggttcaag
     2341 aatcatttct tttagtaata gggttaaaca aaacgattac ctatttgatg tcaagcatcc
     2401 ttttgatgta acgaatttca aattgttcag gttttttatt ttattaaaca atatgttaaa
     2461 ttgcattaat tgcatcattc attcaaagtt atattgccaa ttatcgacca ttctttactt
     2521 cgattagctg tctttttgtg tccacttcgg gagagatact acgtttgaaa tatatttctg
     2581 tcagactatg tgatactttt ccccgggcga attcattttt tgacgtaaaa agagaccgat
     2641 gacggatatt ctttgctacg gatgatctgg atagtagatg agatgaagtc tttcttttta
     2701 catcgatata tatcaaccat ttgttgtgga ttgaggtcga agagtgggaa ccagctactt
     2761 tggatctgta ccatgatgcg atgtcccttt ttgaaggtat gagcaatgtc aggcaaactg
     2821 aaatttacct gttctatttt tcccggtttg aaaccttcag ggtgggagaa tgatttgcgg
     2881 tatctacctc tgaatatttc acctctgacc agcatctggt aacctcccat cggtacatct
     2941 ctgctcgtat attcattgaa atcatccgga aatacatcaa ttaccttgac gacgaaatct
     3001 gcatctgtcg tagtcaattt aacaaaaaga tcagccagaa cgggtcccaa catagttaca
     3061 tctttggtaa ggacatctgt ttgataagtc agtacgtccg gccgttgaga agcaaatctt
     3121 tgatcttcaa tcatgtattc cctgtttctg ttccaactga tgtcgggcct gtaaggtaca
     3181 gggtgtgatg ggtcgcttgt atattggtca taaactcctg attgggttga tttgtcgttg
     3241 cggagattgt tattttccga gagaaataat ctgaattctt caacaccttc gggaggccat
     3301 tggttaaagg cggcccaatg gttagagccg gtgataaata cattgacctt gtctccaatg
     3361 gtaggagtcg gattatcttc caaatatttt ttaaagaagg gaatttcaaa ttcattctgg
     3421 taccattctg ccgttttcga accgaaacga attggcccga attgttctcc actgccccgt
     3481 ccgccccaat tgccatggaa ccaagggccc atgaccaggg tagtattagt ctggggactt
     3541 tttcgagcaa aagatttgta ggtctcccaa gccccataac aatcttcagc atcgaaatgt
     3601 cctcctacaa ccatgatggc cggtttgaca tccttaatat gatgtaaggg gtttctgtct
     3661 tcccagaact tgtccagatt tggatgagca aacaggtcat tccaaaattg aatggaatct
     3721 ccaaaatatt tatgcttaat atctgaaaga ggtcccgcat cgagaaagaa tcgataagca
     3781 tccggtccaa actgggtatt ggaccatcca ctgcccatgg ttggtttagg tcttggtcgc
     3841 ccgaaattaa gataaaaggt aaatgcatcg attaatgcca aagctccgtt atgatggaag
     3901 tcatctcctc gaaaccagtc agtgaccgga gcctgtggac ttacagcctt gatggcagga
     3961 tgtcctgaca gtgcggctac agtggagtag aagccggggt atgaaattcc cattattcca
     4021 actttgccat tattgttttc cagattattg acaagccatt cgacggtgtc ataagtatcg
     4081 gttgcttcat caatatcttg atcagacttt ctttcagcga gtaggggacg gatatcgata
     4141 aaatcgcctt cgctcatgaa ccttcctctt acatcctgtg tgacaatgat gtagttgctt
     4201 cttagataat tcctgaagta accgttataa aattgagcgt agttgtctaa gccataaggt
     4261 ctgcaggaat agggagtacg acgcatgatg atgggatgtg gtccggaaag gtccctgggg
     4321 atatagatac ttgtaaaaag tttggttcca tccctcattg gaatcatttt ttccatcttc
     4381 gtgtagttgt tcctaaacca aagtgaatcc aatgaatcct gggcatttgt cccaacccaa
     4441 catataatga ttacaaaagt cgaaatcaag aatcttttca tgaatacaga aattgaaatt
     4501 tatatagata aatgttcgga aataactgca tagcctgtga atgggcagta gtggcttgca
     4561 gattcaattc cttctgttcg tcttataaga aaccaatgat tttcaaaaat aggatttctt
     4621 tcggaattac ggaatatttg attttgtgta aattttacct gtagatcttt caatactaac
     4681 aggtcttagc ataaggtggc ttttgtcaca atcaagtctc ttgtttgggt gcggctccaa
     4741 aaaatactaa aggctgcaaa tcaattttgc agcctttgga tattaatatt cagagttgtc
     4801 aattgagcag gaacctaaac gtgataccac ctcgctggga gattgttttg aattgttgtg
     4861 aagttttcgg gttggtaagg ttataatcgt agaacagccg gagggtcagg ttcttattgt
     4921 attgatattc gatgtttgga gcgagcctta atgttatagc tccgcgcgat gttctggtaa
     4981 gatcttggtc tataaaatga ctgactgttg catcatcccg ataagataca tccaaccgga
     5041 taatcaggtc attttcggct gctttattac cacctttagg gcctggtcct gtagatgttt
     5101 tggatgggtc ctttccttta tccttctttt tcttgtcatt tttcttaagc tgaaattcct
     5161 tcataaaagg catcttaaca ctatggatgg tatatccggc accaattgtg atatcggatg
     5221 acttggtctc attgaccaga ttatcaacga atgagagtga aagattacgg actttcttgt
     5281 aatcaatatt gaatgtgatg tcgttctttg tctttacatc caaacgaatc aatggagaca
     5341 acccttccgt gatggatacc gccggaatct ccagcctact gtaataattg tttgttatcg
     5401 gattgaagat ataaggatcc tccggatcat agttgggcga agtgttgaaa ctgttgacat
     5461 ttaaggttga cttatatgca tgggtcaggt tgacacttga gaagtatttt ttaaacaact
     5521 ctaacttggt aagaccgttg taggtcaatt gccagttgat cttagggaag gtactgaaga
     5581 tatccagatt ctgttttgtc gggtccgtat tggagtatgc ggccaggaag gccgggatga
     5641 gtacatcctg ttgaatttta ccatagcctg tagtataacc cggtacgttt ggatccggtg
     5701 ttgttcccaa tctattggaa atcacagttc tgttactttc aaatcgtttg aaaacacttc
     5761 tcatatcatc gaagaaggag ttgagggtga agtatgtcgt tgtatatgaa ccgatatccc
     5821 gacctgcata tctaccaaag tcttcaccaa ttgcctttac cttgaatagc tcagtatgag
     5881 tattctggta ggtacgattg gcattcaggt cgattttgaa atcattgaat ggctcgagat
     5941 tgattttagc atcaatggcc tggttctggc caagtataac cagttggttc tggaagatat
     6001 ttttggtaat ccagttgtta ttgggtgcta cagccctttc aaagtaagca tcgtccggtt
     6061 gccagcccgc tatataaccg aagcccggtg ccgaccattg atccattccc atcgaggaag
     6121 tcgaagggac atagcccgga atcgtggagt tcagattttc cgtataggtc aaacgtgcct
     6181 tgcgcaagct tagcagtggt cttatcaaaa tcctttcggc tagagagggt tgataatttt
     6241 ttcttcgttc ttcacgggca agctttctct tttccttttc tttctctttc ctgagtttta
     6301 gcctttctgc ctttgaaagc ttggcatcgg tagaatcctg tttgcccaat gcatccttat
     6361 ttccgggatc atcggttaca ggcttggtgt ttgagccttt ttgttttggc tgtgaaattt
     6421 ttgacgttga tttactttcg ggtttttcaa tttttttcag gtaattccat tttgaatata
     6481 accgatcgaa actaaaatca gcattcaact gtctgat
//
LOCUS       DAOQQN010000168         6456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5098_length_6456_cov_5.648492, whole genome shotgun sequence.
ACCESSION   DAOQQN010000168 DAOQQN010000000
VERSION     DAOQQN010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5098_length_6456_cov_5.648492"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PLG24_07380"
     CDS             <1..851
                     /locus_tag="PLG24_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24749.1"
                     /translation="DPDLQDFVQSTINILSDGIDIDDINGLKLESKGNARRMMSFYDK
                     IKSKSSLWDINISQTQAKNNALNIAAQGKNILTVVDNGSVGICKDDPAFQLDVDGLVG
                     STGRVGNFAIGKVPADGEWHILINDMSGTVGFEAFAHIINDKEDRYGLTYGVLLMSEE
                     KGFKNRIRSVEASSKWLWGKWFNKIVFRWAFDTSNTSVTERRYRLEMKSRTRQGLPGQ
                     ETPYIYYRLAKIWDKQYETGIDLNLTNVHSTTYGRAESVPDEPIRNIPQESVSQPKRS
                     ISIKTK"
     gene            866..1033
                     /locus_tag="PLG24_07385"
     CDS             866..1033
                     /locus_tag="PLG24_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24750.1"
                     /translation="MNKYRLILNAEEVKKILDILFERPFKEVYELIGRINEQLVEQEE
                     YNAESDDEEED"
     gene            1055..3343
                     /locus_tag="PLG24_07390"
     CDS             1055..3343
                     /locus_tag="PLG24_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24751.1"
                     /translation="MPPVLTIKKFPEYSEATDYAGLVRDYLDACQRYSGKVWTDYNVH
                     DPGVTSLELICLAITELGLKSETDIFRIIFNSFKMDFDADDYALYGPLQVFPCTPLTY
                     EDYRKLLVDKFIDVVDNIWIQPNIFTKEGASYKGLLNVLVKPKPGVEKDALSERIYNY
                     FKSIKNIGEDIYKVIILNPVKITINLVASIKGNILPEEVQARLRMLIFNEVERPITFD
                     SYLHALQNGEEINEVLSGPLLENGRIQDASIRNSDLDAQNKIYKSKILHLIAASEFID
                     RVISMDLFVNGVAIDSEGIELTETEFPSFDFYESGFELYKDDNLLYINKETQEYIYKS
                     NYRTKKVFSKKSLVEYLLPNIKSNISLKEITEFYSIQNQFPKIYGISSYGQVQQTEKA
                     RLSRVRFKAYLAMFEQIMQNYLSQLANIDKLFSLKRYGDYTYFYDYPEGIPDFQEVTF
                     DKKEQFIAKLGTIINKYDDRFPRRNMFIDHLCARFGEDLSGNLIAGEYIESTYDLNFK
                     YLKEIKLKSTFLRNYVDLGMYRTKSRMGDSKDNTGLSSLQTRLFILLGIENFQKRIVT
                     KFPDFKEEKTKNKLKVEIKSNEDLKSFFKDAVDLNNYSIEEKKNKYLLKFSMDNAREY
                     KIVESTAYVECENTMEDIRNHSIALNEHCEYFYLVENILLRPEVESLATSDPYSNICS
                     IVVPNWPYRFQSSYFKNNFKNLVLANAPIHIKIDLFFIDYSQMAHFEKLYYQIEEANE
                     KRAFCENEKKALTEFLLSLNAY"
     gene            3345..>6456
                     /locus_tag="PLG24_07395"
     CDS             3345..>6456
                     /locus_tag="PLG24_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24752.1"
                     /translation="MHRIGQITASVKVNDLALGYELNHLLADLLDSDLDGELSALLDE
                     YSTNSLIKLFEQVSIKIEIAEQENHRLRIKEEILRQLREILMKDVYDFENHLSSDRKG
                     EFNIANPNISGSGKVISEVSLLKQYLTRFFQSGSSLELARFRSDELRRQIQQLIDTPS
                     FEFIEYINTLVRQDERATLRIANFFTTHEIEAFLVEIAGRYSFSIVTINQLKDMLLNL
                     TGTDDELGKKLILLSIMAYLKETSEKQLDSEIKHVRKRILSFQISSDTKKSDLIGADK
                     QAGFKSTILQELILFEAYDFSAKVLQNTGKQLKILKEHFPQRDYNLKTDDFPVIQGII
                     ESRQTQPTVQRSAWSYDIRHYLFSSFEFIVQHGTFPFWLRDDESQKLFLQLLRNILDS
                     DVSSFKEKVKNLLQGIHDPMKIYSNIVSGFPSEVRTSLVNTLSENLPDTLQLFRHYYL
                     NFKKRIIEERHVFSAAVKHFFEKDFSEFKMPIIDFMLAGFLAKKTFRTIKEIFSVFLI
                     IHTEEPDGLTGPMEDMNYLFGQDFKVPDGEGEIKSIMDQGRVKSKNVPDNDLIDSTPE
                     RFTKGQNLNFELKIFEDFTENIQEQKAETYMEEYIQWMVDVASIDDYKLPDDIRLFVL
                     HSGMKEMEKRKLAMAEFLHLIFTQDSWLISNKEFLNTAETFYREITSILGDIKDDIGY
                     LKTLQAKIDQHPSDFKEFFVRANNYLGTSLSGFALWKDYIKQAKPSLLFSEDSVNDIR
                     SFLALVSHLDQSDLLSFVSILDHEARGMYARIFMPDAHRPLSFPDKNKLPDQIIFDQI
                     VHSSAGLHFSIDDETIQKAKQFFTKIFRLHRDLKYLRELEGITKDTQPRVVILNILEE
                     RQVADKSLKEIAETSGETTHLFDEALFVQLRSKYGELIAKIGPTELVKRLDTHLSTGE
                     LLKLSDTFISVSDIEFNLKRYVIYVGEKYGVPLHYVALALLDKLLNLGSSQFIFPQYK
                     EVLYMMSFHLYDLALKLINAPKFISKDKEEEKVYNMPAWNLESDENMSIRVTNAGLVI
                     CNPFIVL"
BASE COUNT         2126 a         1229 c         1321 g         1780 t
ORIGIN      
        1 ccgatccgga cttacaggac tttgttcagt ccacaatcaa tattctcagc gatggtatag
       61 atatcgatga tatcaacgga cttaaacttg agtccaaagg caacgcgcga aggatgatga
      121 gtttctatga caaaatcaag agtaaatctt cgctatggga tataaacatc agtcagactc
      181 aagccaaaaa caacgcattg aacattgctg cccaaggcaa aaatatcctt actgtcgtag
      241 ataatggcag cgtggggata tgcaaggatg atccggcatt tcagctcgat gtggacgggt
      301 tggttggctc aaccggaaga gtcggcaact ttgcgatagg gaaggtgcct gccgatggtg
      361 agtggcatat cctgatcaat gacatgtcag gaactgtagg ctttgaggcc tttgctcaca
      421 tcattaatga caaggaagat cggtacggcc ttacttatgg ggtcttgctc atgtcggagg
      481 aaaagggttt caaaaatcgt atccgttcag tagaagcgag cagtaagtgg ctgtggggca
      541 aatggttcaa caagattgtc tttcgatggg cttttgacac ctccaatact tcggttactg
      601 aaagaaggta tagattggag atgaaatcca gaacaaggca aggtcttccc ggccaggaaa
      661 ctccctatat ctattaccgt ctggcgaaaa tctgggacaa acaatacgaa accggcatcg
      721 atctcaacct gacgaacgtc cacagtacaa cctatggaag agcagaaagt gtgccggatg
      781 agccgataag aaatattcct caggagtcgg tatcacaacc caagagatcg attagtatca
      841 agacaaaata agcggataga atctgatgaa caaatacagg ctgatattga atgcggaaga
      901 ggttaaaaaa atccttgaca tccttttcga gagacctttc aaggaggtat acgaattgat
      961 tggtaggata aatgaacagc tcgtggaaca agaagagtac aatgccgaaa gcgatgatga
     1021 agaggaagat tgataattac taaatgagta tattttgcca cccgtactta ccataaagaa
     1081 atttccggag tattccgagg ctactgatta cgcaggactg gtcagggatt atcttgatgc
     1141 atgtcaaaga tattccggca aggtatggac ggactacaat gtccacgatc ccggagtgac
     1201 atcattggag ttgatttgcc ttgccatcac ggaactgggg cttaagtcgg aaactgatat
     1261 tttcaggatc atcttcaatt ctttcaaaat ggatttcgat gcagatgact acgccctgta
     1321 cgggccattg caggtatttc catgcactcc attaacctat gaagattatc gaaaactgct
     1381 ggtcgataaa tttatcgatg tggtggacaa tatttggata caacccaaca tatttacaaa
     1441 ggaaggtgct tcatacaaag gccttctcaa tgtattggtc aagcctaagc cgggtgttga
     1501 aaaggacgca ttgtcagaaa ggatttataa ttattttaaa tccatcaaaa acataggtga
     1561 agacatttat aaagtaataa tcctcaatcc agtaaaaata accatcaatc ttgtcgcatc
     1621 catcaaagga aatattctcc cggaagaggt tcaagccaga ttgagaatgc ttatctttaa
     1681 tgaagtggaa cggccaatca cctttgacag ctacctgcac gcactgcaaa atggagaaga
     1741 aatcaatgaa gtcctgtccg gcccactgct ggaaaacgga agaatacaag atgcttccat
     1801 caggaactcg gaccttgatg cacagaataa aatttacaag tcaaagattc tgcacctcat
     1861 agcggcttct gaatttattg acagagtcat ctccatggat ttgtttgtca atggtgttgc
     1921 gatcgacagc gaaggtattg aactcaccga gaccgaattt ccctcttttg atttttatga
     1981 atccggattt gaactataca aagacgataa ccttctatac atcaataaag aaacccagga
     2041 gtatatatac aaaagcaatt atagaaccaa aaaggtgttc agcaagaaaa gcctggtgga
     2101 atacctgctt ccaaatataa aatccaatat atcactcaaa gaaattacag aattctatag
     2161 tattcagaac caatttccga aaatctacgg tatctcctct tatgggcagg tgcaacaaac
     2221 agaaaaggcc aggctgagtc gtgtacgatt caaggcttat cttgccatgt ttgaacagat
     2281 catgcagaac tatctttcac aacttgccaa tatagataaa ttattctccc tgaaaagata
     2341 cggcgattac acttattttt atgactaccc tgaaggaatt ccggattttc aagaagtaac
     2401 ctttgataaa aaagaacaat tcatcgcaaa actgggtacg ataatcaaca aatatgatga
     2461 tcgttttcca agacggaata tgtttatcga tcacttatgt gcacgatttg gagaggatct
     2521 ttcgggaaat ctgatagccg gcgaatatat agaatccacc tatgacctga atttcaagta
     2581 cctgaaagaa ataaaattaa agtccacctt cttgcgtaat tatgtcgatt tgggaatgta
     2641 ccgaaccaaa agccgcatgg gagattcaaa agacaatacc ggattatcat cattgcagac
     2701 aaggttgttt attctgctgg gcattgaaaa ttttcaaaag cgcatcgtaa ctaaattccc
     2761 ggattttaaa gaagaaaaaa caaaaaataa attaaaggtc gagattaagt ccaatgaaga
     2821 tctgaagtct ttctttaaag atgcagtcga tttaaataat tatagcattg aagaaaagaa
     2881 aaacaaatat ttgctaaaat tttcaatgga caatgcgaga gaatataaaa tagtcgaatc
     2941 aactgcctat gtcgaatgtg aaaataccat ggaagacatc agaaaccatt cgattgctct
     3001 gaatgaacat tgtgaatatt tctatctggt cgagaatata ttgcttagac ctgaggtaga
     3061 gagccttgcc accagtgatc cctattccaa tatttgcagt attgttgtgc ctaactggcc
     3121 ttacaggttt caaagttcat atttcaaaaa taatttcaaa aacctcgtcc ttgcaaacgc
     3181 cccgatacat ataaaaatag atctattctt catagattat tctcagatgg cacacttcga
     3241 aaagctatat tatcaaattg aggaagcaaa cgaaaaaaga gccttctgcg aaaatgaaaa
     3301 aaaggcactt accgaatttt tactttctct gaacgcttat tgacatgcac aggataggac
     3361 aaataaccgc ttcggtcaag gtgaatgatc ttgcactcgg ttatgagttg aatcatttgc
     3421 tggcagacct tttggatagc gatttggacg gtgaactgag tgcattgttg gatgaataca
     3481 gcactaatag tctgattaaa ttgtttgaac aggtatccat caagatagaa atagctgaac
     3541 aggaaaatca tcggctcaga attaaagaag aaatcctgcg tcaattaaga gaaatcctca
     3601 tgaaggatgt atatgatttt gaaaatcacc tcagttctga cagaaaggga gaatttaata
     3661 ttgcaaatcc taatatttcc ggctcaggga aggtcatttc agaagtaagc ctcctcaaac
     3721 aatatctaac ccggtttttt caatcaggtt cttcccttga gcttgcaagg tttagatccg
     3781 acgagttgcg ccggcagata caacaactaa tcgacacccc ttcctttgaa tttatcgagt
     3841 acatcaatac gcttgtcaga caggacgaaa gagctacatt gcggattgcc aattttttca
     3901 caacacatga aattgaagca tttttagtag aaatagcagg cagatattct ttttccattg
     3961 tgacgatcaa tcaactaaaa gatatgctat tgaacctgac cgggactgat gatgagctgg
     4021 gaaaaaagtt gatcttattg tcaatcatgg catacctcaa agaaacctca gaaaaacaac
     4081 tggattcaga aataaaacat gtaagaaagc gaatactctc cttccaaatt tcctccgata
     4141 ccaaaaaatc agatctgatt ggagctgaca aacaagcagg atttaaatcg acaatattgc
     4201 aggaattgat attatttgaa gcttatgatt tttccgcaaa agtattacag aataccggca
     4261 agcaactaaa gatacttaaa gagcacttcc cgcaaagaga ctacaatcta aaaaccgatg
     4321 attttcctgt aattcaaggt ataatagaat cccgacaaac tcagccaacg gtccaacgat
     4381 cagcatggag ttatgatatc aggcattatt tgttttcttc ctttgaattc attgtgcagc
     4441 atgggacctt ccccttttgg ctcagggatg acgaaagtca gaagcttttt ctccaattgt
     4501 tgcgaaatat attggacagt gatgtcagtt cgttcaagga aaaggtaaag aatcttctac
     4561 agggaataca cgacccgatg aagatatatt ccaatatagt ttccggcttt ccttctgaag
     4621 tcaggacttc ccttgtaaac actttgtctg aaaacctgcc ggacacattg caacttttcc
     4681 ggcattatta tttaaatttc aaaaaacgca tcattgaaga aagacatgta ttttccgctg
     4741 ctgtaaagca cttttttgaa aaggatttca gtgagtttaa gatgccgatt attgacttca
     4801 tgctggctgg ttttttagca aagaaaacat ttaggaccat taaggagatt tttagtgtat
     4861 tcctgataat tcatactgaa gagccggatg gattaaccgg accaatggaa gacatgaatt
     4921 atctattcgg acaagacttc aaggtccctg acggagaagg tgaaatcaaa agtataatgg
     4981 atcaaggcag ggtgaagtct aaaaatgtac cggacaacga cttaattgat tcaactcccg
     5041 aacgctttac caagggtcag aatctaaatt ttgagctgaa gatcttcgaa gattttactg
     5101 aaaatattca ggaacagaag gccgaaacat acatggagga atatatacaa tggatggtgg
     5161 atgtagcttc cattgatgac tataagctgc cggatgatat cagactattt gttttgcata
     5221 gtggtatgaa ggaaatggaa aaacggaagt tggctatggc tgagttttta catttgattt
     5281 ttactcaaga cagctggctt attagtaata aagaatttct gaacactgca gaaacattct
     5341 atcgggaaat cacctccata ttgggagata tcaaggatga tatcggttac ttaaagacat
     5401 tacaggcaaa gattgaccaa catccttctg atttcaagga gttttttgtt cgggcgaata
     5461 attatttagg aacaagcctg agtggctttg ctttatggaa agactacatc aaacaagcga
     5521 aaccttcatt attgttttct gaagattctg tcaacgatat aaggtctttt cttgccttgg
     5581 tttcgcacct tgatcagagt gatttgctct cctttgtttc aatcttagat catgaagcac
     5641 gtggcatgta tgcaagaatt ttcatgccgg atgcccatcg tcccctctcc ttccctgaca
     5701 agaataaatt gcctgatcag ataattttcg atcagattgt ccattcaagt gccggcttgc
     5761 atttttcaat agatgatgag acaatacaga aggcaaaaca attttttact aaaattttcc
     5821 ggctacaccg cgatttaaag tatctgaggg agttggaggg aattacaaag gatactcagc
     5881 cccgagtagt gatccttaat attttagagg aaaggcaggt tgcagataag agtttgaagg
     5941 aaattgccga aacatccgga gaaacaacac atttgtttga tgaggcatta tttgtccaac
     6001 tacgatccaa atacggtgaa ttgattgcca aaatcgggcc gacagaactc gtcaagagat
     6061 tggataccca tctgtcaaca ggcgaattat tgaaactttc cgatactttt atctcggttt
     6121 ctgacatcga gttcaatctg aagaggtatg taatatacgt tggagagaag tacggcgtcc
     6181 cactccatta tgtagccctg gcgctgctcg ataagctact caatcttggg tcttctcaat
     6241 ttattttccc gcaatacaag gaagttttat acatgatgag tttccatctg tatgatctgg
     6301 cccttaaact tatcaacgcc cccaaattca tatccaaaga caaggaagaa gaaaaagttt
     6361 acaatatgcc tgcatggaac ctcgaatccg atgaaaatat gagtatccgg gttaccaatg
     6421 ccgggcttgt tatctgtaat cctttcattg ttctgc
//
LOCUS       DAOQQN010000169         6446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5109_length_6446_cov_5.735566, whole genome shotgun sequence.
ACCESSION   DAOQQN010000169 DAOQQN010000000
VERSION     DAOQQN010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5109_length_6446_cov_5.735566"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PLG24_07400"
     CDS             complement(<1..481)
                     /locus_tag="PLG24_07400"
                     /inference="COORDINATES: protein motif:HMM:NF018648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HMZ24753.1"
                     /translation="MHKRNNKLNPFLAFIDKEKVGSILLGISVILALIIANSPLAPYY
                     YEILDKKLGLSIDGRMFQEHDILHWINDGLMAVFFFVVGLELKREFVGGELASPRQAL
                     LPIGAAIGGMVVPALIYIIFNYGTETAHGWGIPMATDIAFALGVLHLVGNRVPIGAKV
                     "
     gene            complement(638..2527)
                     /locus_tag="PLG24_07405"
     CDS             complement(638..2527)
                     /locus_tag="PLG24_07405"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HMZ24754.1"
                     /translation="MNLRTGLIFVFLAFYLARANAIQGVLPEYERILSFRSEIVIDSS
                     GKLIVEETILVNALNISINRGIFRALPTERNVNDHTIQVSYQIISIKKNGVEEPYHDQ
                     YENGYKIIYIGDEDIYLSPGIYQYKITYSTERQIGFFDHYDELYWNITGNKWAFPIDT
                     AIATVTLPEAAQILQSSCYTGSYGSTSRNCNAEQKTPNSMTWTAFNLGTNEGLTVAVG
                     FTKDIVSRPVIPDSMKPIALMKWFLYVGLFLFVIMAWLWYRHGVDLPAPAVYPQFDTP
                     EGLSPAAIGYLEGGKYRNTLMAASLINLAIKKLIKIEEVPKSGFFGRSKYIITRLDTQ
                     DVPLSTEESNVLYNMVGYKGSTLELDGTYDSKVQQTVQSFKDSIKQQYNSIIKEGSNR
                     KYVFFVFLFISLAYLPVLNLIHKTYYYAGEQSNGAGFFVVYIILYFIAHFSGYRTSRW
                     IWLIPLLLTIALFYFILTSRHYINSYWVLILFYGMIMTGISFFSYAIRQPGKRFLELQ
                     SLIAGFRMYLGAAENQLIKFHNPPKMTPEVFEKYLPYALVLGVDNIWGRKFETALKEQ
                     GKEYQNQWYVGPNSFSNSFAGSFSQNLSSRMTSASTQPSSSSSGSGGGGFSGGGGGGG
                     GGGGW"
     gene            complement(2532..3086)
                     /locus_tag="PLG24_07410"
     CDS             complement(2532..3086)
                     /locus_tag="PLG24_07410"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HMZ24755.1"
                     /translation="MKFLLFFFLLTVVILFFFISIYNKLVRAKNLMLEGWSGIDVFLK
                     KRHDLIPNLVETVKGYATHERETLDSVVQARAQALNAGSISEKQAAENNLGKSLVNLF
                     AVAEQYPDLKANANFQALQGQLVNIEGDIEKARRYYNGTARDYNIAIESFPGNILAGA
                     FTFGPASYFEIEDSGERSVPQVKF"
     gene            complement(3399..3620)
                     /locus_tag="PLG24_07415"
     CDS             complement(3399..3620)
                     /locus_tag="PLG24_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC family transcriptional regulator"
                     /protein_id="HMZ24756.1"
                     /translation="MEELNMKDVLEKSAFGVCTYIGEKVGIATSRVRLYFIYLSCATL
                     GSSMILYLFMAFWVNIRKSLKRNDRTAWE"
     gene            4461..4543
                     /locus_tag="PLG24_07420"
     tRNA            4461..4543
                     /locus_tag="PLG24_07420"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4495..4497,aa:Leu,seq:gag)
     gene            complement(4624..>6446)
                     /locus_tag="PLG24_07425"
     CDS             complement(4624..>6446)
                     /locus_tag="PLG24_07425"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HMZ24757.1"
                     /translation="KTSGMFYVNYTEKDEGNTIIARYRISSDSNRAVKASEEILLEID
                     QPFANHNGGNIEFGHDGYLYIGMGDGGSGGDPQDNSQNKQKLLGKMLRIDVSSSPGYD
                     IPADNPFVGDSEYRPEIWSLGLRNPWRFKFDELTGDLWIADVGQNKWEEIDFEEAGDG
                     GRNYGWRCYEGYVPYNTAGCGDILGYTFPVAAFSHDEGHCSISGGVVNRKQPNSSLYG
                     YYYSVDYCSGVFWGTKRNEDKSFTTIELARTNIDGFVAFGYDKDKNIYAARDNGQIYR
                     IDTFLLCAPELVIEGTDTEVGCKIDSVVLSTQEISNGQYTWFLDDEPINGSNASTIVV
                     TKPGKYSVQFDSEECSATSNVDYVLEQNKEVDVTILDVPENYCFNGAPLVFHGQPAGG
                     GFFGTGMVDSVFYPSISDIGTFFITYSYEDEEGCNGFDVGVITVNHPPGTKILSTPPQ
                     LCQFATPYVLEATPAEGSWSGPGLTGSTFYPYLAGVGVHKIKYTYNPWPGCFAYDSIY
                     ITVIDCTNGVGEADSPEISISPNPVKDKVVVALKNRNYNPENISIINSLGKEVIRKKL
                     DTHNSAEKLVELSVEGLTPGVYHVIINVGEKRLQRKIIKI"
BASE COUNT         1964 a         1501 c         1176 g         1805 t
ORIGIN      
        1 acaccttcgc cccaatagga acccgattgc caacaaggtg cagtacgccc aatgcaaatg
       61 cgatgtcagt agccatcgga attccccagc catgggctgt ttctgtaccg taattgaata
      121 tgatataaat caaggcaggt acgaccattc cgccaattgc ggctcctatt ggtaataaag
      181 cctgacgggg cgatgccagt tcaccgccta caaattcccg ctttagctcc agtcccacta
      241 caaagaaaaa aacagccatc aacccatcat tgatccaatg caggatatca tgctcctgaa
      301 acatcctgcc atcaatcgag agccccaatt ttttgtccaa tatttcgtaa tagtaaggcg
      361 ctaaaggact attggctatg ataagtgcca ggattacact tatgcccaac aaaatactcc
      421 caaccttctc cttatcgatg aatgccagaa aggggtttaa cttattattt cttttgtgca
      481 taatgaattg ttttaataca cgactgatac aaaaccatta catgagtaag aatcaatcga
      541 accatgcaaa ccaatttttc cgccaattat aatccaaagt aaaataaaaa aattggtttt
      601 cccaatatta aaaccaattt ttccaaatat tatagggtca ccagccacca ccgccaccac
      661 cgccgccacc gccgccggag aagcccccac ctcccgatcc gctggatgaa gaacttggct
      721 gggtactggc agacgtcatc ctgcttgaaa gattctgact aaaactgccg gcaaaactat
      781 tggagaatga atttggacca acataccatt gattctgata ctcctttccc tgctctttga
      841 gtgcagtctc gaacttcctg ccccagatat tatcgacacc cagaactaaa gcatagggaa
      901 gatacttctc aaaaacttca ggcgtcatct tcggaggatt atgaaatttg atgagttgat
      961 tctctgcggc accaagatac atcctgaaac cggcaatcaa ggattgaagt tcaaggaacc
     1021 tcttaccggg ttgacgaatg gcataactaa aaaaggaaat tcccgtcata atcatcccat
     1081 aaaataaaat caatacccaa taagaattga tataatgcct tgatgtcagg ataaaataaa
     1141 ataatgcaat agtcaacaac aaaggtatta gccaaatcca tcgcgatgtg cgatatcccg
     1201 agaaatgggc aatgaagtaa aggataatat atactacgaa aaaaccagca ccattacttt
     1261 gttctccggc ataataataa gttttgtgga taagattcaa aaccgggagg tacgcaagac
     1321 taataaacag aaagacaaag aacacgtatt tcctgttgga tccttctttg ataatcgaat
     1381 tgtattgctg tttgattgaa tccttgaatg actgtactgt ttgctgtact ttggaatcat
     1441 aggttccatc gagttccaat gtcgagccct tgtatcccac catgttatac agaacattcg
     1501 attcttctgt actcaaagga acatcctgcg tgtccaatct tgtgataatg tatttacttc
     1561 taccgaaaaa gcccgatttg ggaacttctt cgattttaat caacttttta atggcaagat
     1621 tgatcagaga agcagccatc aaggtatttc tgtattttcc gccctcaaga tagccaatcg
     1681 ctgccggact caggccttca ggagtatcaa actggggata gactgcagga gcaggcaaat
     1741 ctacaccatg tcggtaccac aaccaagcca tgataacaaa caagaacaga ccgacataca
     1801 aaaaccactt catcaatgca atgggtttca tgctgtccgg gataacaggt ctggatacaa
     1861 tatccttagt gaagccaaca gcaactgtca gtccttcatt tgttccaagg ttgaaggctg
     1921 tccatgtcat agaattggga gttttttgtt ctgcattgca attcctggaa gtactgccat
     1981 aagatcctgt ataacaggat gattggagaa tctgtgccgc ttcgggaagc gtaactgttg
     2041 caatagcagt atcaatcgga aatgcccatt tattgccggt tatgttccaa tacaattcgt
     2101 cataatggtc aaaaaaaccg atttgtctct cagtactata ggttatctta tattgataaa
     2161 taccaggact taagtagata tcctcatcgc cgatataaat aatcttgtaa ccgttttcat
     2221 actgatcgtg ataaggctct tcaacaccgt ttttctttat ggagatgatc tgataactta
     2281 cttgtatggt atggtcattg acattccgct cagtgggaag ggccctgaat ataccccgat
     2341 tgatgcttat atttaaagca ttgacaagaa tcgtttcctc tacaatcaat tttccggaag
     2401 aatctatgac aatctccgac ctgaagctta aaatacgctc atattccggc aagacacctt
     2461 gaatagcatt agctctggca agataaaatg ccagaaagac aaatatcaat cctgtccgga
     2521 gattcatagg atcaaaactt aacctgcggt acgcttcttt ctcctgaatc ttcaatctca
     2581 aaataagagg ccgggccaaa tgtgaaagct cctgcaagaa tattccccgg aaaggattca
     2641 atagcgatgt tgtaatcgcg tgctgtacca ttgtagtatc gccttgcctt ctcaatatca
     2701 ccttcaatat tgactaactg cccttgcagg gcctgaaagt tggcattggc tttcaggtcc
     2761 ggatattgtt ccgctacagc aaaaaggtta acaagagact ttcccaggtt attctctgcc
     2821 gcctgctttt cacttatgga tcctgcatta agtgcctgtg ctctggcctg caccacggaa
     2881 tccagtgttt cccgctcatg ggtagcatat cccttgaccg tctcaacaag attgggaatc
     2941 agatcgtgcc tctttttcaa aaacacatcg atgccgctcc aaccttccag cattaggttc
     3001 ttggcacgca ccaacttgtt atagatactg atgaagaaaa agagtatcac aacggtaagt
     3061 aggaaaaaaa agagtagaaa tttcataaca ctaaagttta agcaaccaaa tataaacaaa
     3121 aatcgtgtaa ttctcggttt tatacattgg aatatcattt ttccgtgctt acaagcttta
     3181 aaggacttag attgcctgat aaagctccgg atgtgccgag taaaaatttt tgaaatgggt
     3241 cttggataag aaagattcaa gtcggctacc ttaccgggct aaatggcagg taaaataatt
     3301 acaaaaaggg agaaatgcgg ggcatttaat catagggaat aaaaccgcca tatacaggta
     3361 atttcaagat tccttatcgt ataattcaaa atagcaacct attcccaggc tgttctgtca
     3421 tttcttttta aggacttgcg tatattgacc caaaacgcca tgaaaagata tagaatcatg
     3481 gaggaaccca gggtagcgca actaagataa ataaaatata gtcgaacgcg tgaagtagct
     3541 atccctactt tctctccgat ataagtacag acaccaaatg ccgacttctc aaggacatcc
     3601 ttcatgttca attcttccat tgctatgtat ttaatccatg catagaaact acaaatttac
     3661 cataaatcac agaatttcca ataaataacg cagattgtgt ggataaaaaa tgtacttttt
     3721 actcacgttt agatgtaaag atgcctggac cctaaaagca aaatcaaata gtatcagata
     3781 ttcaattggt cataaccaat cgtagagtac catattgcaa tttatttttt cttgctataa
     3841 aaataatgcc atatgacatt cccaatagtc caatttcatc gacccaaacg actgctacaa
     3901 cggcatctcc ggaccccgga ttgtttcacc cccccggaca gagcaaagtt ctggcgctta
     3961 gctctcggga caagtatcca agtatttttt atactgtttc atgattctcc ttggtataac
     4021 aagaatcaag gccatgtaga atactttgtg cttttatgaa aataaatcat tcaaaaaaaa
     4081 cttaacgcac tgtatatatg actccacgct cacttcaggg tatatccttt tgttattttt
     4141 ttgaccattt acctacgcat ccgttaccgc aggaaccaat tatggtaggg tttattttac
     4201 ctttagttca atctccggtg aaattgtagg cttgttataa ggccggatcc atgcagaact
     4261 tactttcgga caatgcttga tttttatagt tgtgcaagat atcgtacaca tctttttaat
     4321 ttaattatgg ttgaaataaa atagattttc tatcttcacg cccgtttttg aataaatcat
     4381 ttcaaaaacc taccggaaaa agtcaccggc agaaatacga taaatagcat cctattatcg
     4441 tgaaacattg acaccatcac gcccacgtga tgaaattggt agacatgcaa tcttgagggg
     4501 gttgtggact atgtccgtgc cggttcgaat ccggccgtgg gcactactta gaaaagaaat
     4561 tccataaaac aaaacaggcc cggaaagatt aaaccggacc tgttttgttt tatggaatac
     4621 agattaaatc ttgattatct ttctctgcag cctcttttct cctacattta tgatgacatg
     4681 atagactccg ggcgtcaatc cttctacgga taactctaca agtttttcag cagaattatg
     4741 ggtatcaagt tttttcctga taacctcctt tcccagacta ttgatgattg atatattttc
     4801 cggattgtaa tttctatttt tcaaggctac caccaccttg tccttaacag gattgggcga
     4861 aatactgatc tcgggtgaat cagcctctcc cactccattc gtgcaatcaa tgactgtgat
     4921 atagatacta tcataagcaa aacaaccggg ccatggattg taggtatact tgattttatg
     4981 cacaccgaca cctgccagat aaggatagaa tgtgcttccg gttaagcccg gcccactcca
     5041 cgaaccttct gccggagtag cctccagtac ataaggcgtt gcaaactgac ataattgagg
     5101 tggggtagac aggatcttcg taccgggcgg atggtttacc gtaatcacac ccacatcaaa
     5161 accattgcag ccctcctcat cctcatagga ataggttatg aaaaaggtcc cgatatcgct
     5221 gatggaagga taaaacaccg aatcaaccat acccgtacca aaaaatccgc cacctgcagg
     5281 ttggccatga aatacaagtg gcgcaccatt aaaacaataa ttctccggca catcaagaat
     5341 cgtcacatca acttccttgt tttgctcgag cacataatct acatttgatg tagcactgca
     5401 ttcctccgaa tcgaattgta ccgaatattt gcctggttta gtaacaacaa tcgtacttgc
     5461 gttgcttcca ttgataggct catcatccaa aaaccaggtg tactgcccat tgctaatctc
     5521 ttgtgtggaa agaacgacgg aatcaatctt acatccaact tcagtgtcag tgccctcaat
     5581 gacaagttcc ggggcacaca gtaagaaagt atcgattcgg tagatctgtc cattatctct
     5641 tgctgcataa atattcttat ccttatcata accaaatgcc acaaagccat cgatattggt
     5701 ccgggccagt tctatggtcg taaaggattt atcctcattg cgcttggtcc cccagaatac
     5761 tccggaacag taatctaccg agtaataata accatacaag gaactattcg gttgcttcct
     5821 gttcacaacc ccacctgaga tgctacaatg accttcatca tgactaaatg ctgctacagg
     5881 aaaagtatag cctaatatat caccacaacc agccgtatta taaggcacat aaccctcata
     5941 acaccgccat ccgtagttac gtcctccatc cccggcttct tcaaagtcta tctcctccca
     6001 cttgttttgc ccgacatcgg cgatccaaag gtcgcctgtc aattcatcaa atttgaatct
     6061 ccacggattc ctcagaccca atgaccagat ctccggccta tattccgaat ctcctacaaa
     6121 cggattgtcg gcgggaatat catatcccgg actgctactc acgtcaatcc gcaacatttt
     6181 gcctagcaat ttctgcttat tctgagagtt gtcctgtgga tcaccgccac ttcccccatc
     6241 acccatacca atgtacaaat acccatcatg accaaactca atatttcctc cattatgatt
     6301 ggcaaatggt tgatcaatct ccaggagaat ttcctctgag gctttgaccg ccctattgga
     6361 atcagatgaa atacggtacc gggcgatgat ggtgttacct tcatccttct cggtataatt
     6421 gacatagaac atgccactcg tcttgt
//
LOCUS       DAOQQN010000170         6438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5125_length_6438_cov_4.684004, whole genome shotgun sequence.
ACCESSION   DAOQQN010000170 DAOQQN010000000
VERSION     DAOQQN010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6438
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5125_length_6438_cov_4.684004"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PLG24_07430"
     CDS             complement(<1..306)
                     /locus_tag="PLG24_07430"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HMZ24758.1"
                     /translation="MQLFWGKRINDNVILVEGDEVLHLTKSLRKKVGDAVDLIDGSGN
                     IFHSVITEIQRGKLVAVNMSETPKVNHWKKRLHIAIAPTKNADRMEWFVEKAVEVGID
                     "
     gene            433..1770
                     /gene="rseP"
                     /locus_tag="PLG24_07435"
     CDS             433..1770
                     /gene="rseP"
                     /locus_tag="PLG24_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HMZ24759.1"
                     /translation="MDVIVMIAQLILSLSILVILHELGHFIPAKLFKTRVEKFYLFFD
                     PWFSLFKFKKGDTEYGVGWLPLGGYVKISGMVDESMDKEFMKRPPEPWEFRSKKAWQR
                     LIIMIGGVTVNFILGFLIFGLILYFYGEQKLPVSEMQYGIATTQLAKDIGLKDGDKIL
                     QIGNNDFKYFNSGELAKDILLDNAKTIRVNRNNLIMTVHIPDSAVQKIQSNQKEAIGM
                     IAPRMPIIVDEVMQGSGGEKAGLQPNDRVISVNNVSTHFFHEMTNELQANKGKKVNIG
                     VIRDGNTVMLEQVQLSDQGLVGFSPQPISHFYKLDTIKYSLGKALPAGFRNGYDFLAT
                     QISAFGQMFSGKIKASESLGGFASITKLFPTEWDSQKFWNITAILSLILGFMNLLPIP
                     ALDGGYIMFLLWEVITGRKVNDKVMEIATTAGMILLIGLLLYANGLDVIRALK"
     gene            1770..2282
                     /gene="hscB"
                     /locus_tag="PLG24_07440"
     CDS             1770..2282
                     /gene="hscB"
                     /locus_tag="PLG24_07440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HMZ24760.1"
                     /translation="MDYFEWFGLDRKFNIDQAGLRKAFLTKSMELHPDKTGLNENKAM
                     ELSEYNNKAYGILKDSYKTLDYILHLEGIVLADEKYQLPQDFLMEMLELNMEIEDAGE
                     SGDKEKLNKIKSDLETKGIEEDANMEILKNQYDSRSIDPQGYESLKKFYFIRKYLLRI
                     EESINTFASH"
     gene            2314..2397
                     /locus_tag="PLG24_07445"
     tRNA            2314..2397
                     /locus_tag="PLG24_07445"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2348..2350,aa:Leu,seq:caa)
     gene            complement(2760..4118)
                     /locus_tag="PLG24_07450"
     CDS             complement(2760..4118)
                     /locus_tag="PLG24_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HMZ24761.1"
                     /translation="MYFKNILETIGNTPLVRINKITSDLPCTVLAKVETFNPGHSIKD
                     RMALKMVEVAESEGKLKPGGTIIECTSGNTGMGLALVAAVKGYKAIFTTSDKQSKEKM
                     DMLKAMGAEVIVCPTNVEAADPRSYYSVARRLSQEIPNSYWFNQYDNPANSQAHYETT
                     GPEIWEQTEGKITKLVVGVGTGGTVSGTAKFLKEKNPDIEIIGIDTYGSVFKKYKETG
                     IFDENEIYPYITEGIGEDILPENVDFNLIDYFEKVTDKDGALMARRLAREEGLLLGYS
                     AGSAMAGLLQVKNRFRPDDVVVIIFHDHGSRYVAKIFNDDWMRDRGFISMDLRLSDLV
                     KRKPKSGLITAQKGSKLREVFQMMKENDISQVTVNDQGEIVGSLTESDIISSLLSNPD
                     NIDNVSVGEIMNEPFPVLEEALSVKELSRYISKKIPAVLVRDGAGVNHILTQYDIIQH
                     LG"
     gene            complement(4328..5344)
                     /locus_tag="PLG24_07455"
     CDS             complement(4328..5344)
                     /locus_tag="PLG24_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HMZ24762.1"
                     /translation="MPSRKLLYVVSWVFVISLFSQKAGATGDSLSVLTKNDTIFIQRD
                     SLGVIYFYHRVMPKQTMYSLCRFYGIKQSQLMTLNPVLKVRGIQIDELIRVPISARLI
                     RVESEPKKRENFAPVVYEVSPGEGLYSVSRNYFGIKPEKLMQINGMSAPDLKLGQILL
                     LGWIPLDVFNKINENVVADKGRPKSGTKADNAKTDTKKPGPNALGTTSTAKEREIAKE
                     AVQDAVEKTTSEEAKSTPASNREKFESYEGTKAFEQGLAFWHKELKGSNGLFVLHRTA
                     PRGSIIKITNPMFNSTVYAKVVGTVSPNSYSDDVMMVVSPEVAIKLGARDARFFAKIA
                     YIKG"
     gene            complement(5824..>6438)
                     /locus_tag="PLG24_07460"
     CDS             complement(5824..>6438)
                     /locus_tag="PLG24_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ24763.1"
                     /translation="QQNRLELFAVTAGLTHLKHSFPDLFDDVEIDYDLNGLFKYIVMD
                     QNDLKMVVIGNFDVTDGSKAITFPTDEWYYNYMGRDSIKGNTSLRQITLKQGEYKVYL
                     NKKVENPGITLSDKGTLIQDVEFTVYPNPAESELHIQLPERIFYETVSVTVSDISGRI
                     VYHDNRLSGQTTITIPLNSVKPGVYLVRVSNPSVFGVKKVMIVR"
BASE COUNT         1951 a         1325 c         1273 g         1889 t
ORIGIN      
        1 atctattcca acttcaacgg ctttctcgac aaaccattcc attctgtcag cattcttggt
       61 gggcgctatt gctatatgga ggcgtttttt ccaatgatta accttaggag tctctgacat
      121 attcacggcg accagcttac ccctttgaat ttcagtgatg acggaatgaa agatattgcc
      181 ggacccgtca atcaagtcaa cagcatctcc tacctttttt ctaaggcttt tggtcaaatg
      241 aaggacctca tcaccttcta ccaaaatgac attatcattg attcgtttcc cccaaaatag
      301 ctgcataatc acttaaatta cctgcaaatc tttagataaa gctaaagaaa cttttacctt
      361 tgcgctcttc gaaacacaac taaatattca aaacagtgtt ttgacatggt aaaaataaaa
      421 taaaagtaat taatggatgt aatagtcatg atcgcccaac tgatattgag cttatcgata
      481 ttggtaatct tacacgaatt agggcatttt attccggcca agttattcaa gacaagggta
      541 gaaaaatttt atctgttttt tgatccctgg ttttcgttgt tcaaatttaa aaaaggagat
      601 acagaatatg gtgtaggatg gttacccttg ggagggtatg ttaaaatatc aggtatggtg
      661 gatgaaagca tggacaaaga gtttatgaaa aggccaccgg agccatggga gttccgttct
      721 aaaaaagcct ggcagcgtct cataatcatg attggaggtg tgacggtcaa ttttatttta
      781 ggttttctga tcttcggatt gatattgtac ttttatggtg agcaaaaact gccggtaagt
      841 gagatgcaat atggaatagc caccacccaa ttagctaagg atatcgggct gaaggatggt
      901 gataaaatcc tgcaaatagg aaataatgat ttcaaatatt tcaattcagg agaactggca
      961 aaggatatcc tgctggataa tgccaaaacg atccgggtca acagaaataa tctgataatg
     1021 accgtacata ttccggacag tgcagtccag aaaatacagt ctaatcagaa ggaagcaatt
     1081 ggcatgattg caccgcggat gcctattata gtcgatgagg tcatgcaggg atccggtgga
     1141 gaaaaggccg gactccaacc taatgaccgt gtcatcagtg tcaacaatgt ttcaacacac
     1201 ttttttcacg agatgaccaa tgagttgcaa gcaaataaag gcaagaaggt taatataggt
     1261 gtcatcagag acggcaatac tgtgatgctg gaacaggtgc aactctctga tcagggcttg
     1321 gttggcttca gtcctcaacc aatatctcat ttttataaat tggacaccat caaatactcc
     1381 ttgggaaagg ctcttccggc agggtttcgc aatggttacg acttccttgc tacacagatc
     1441 tccgcttttg gccagatgtt cagtggaaag atcaaggcgt cagaatcatt gggaggattt
     1501 gcaagtatca ctaaactgtt cccgacagaa tgggacagtc aaaagttttg gaacatcact
     1561 gccatattgt ctctgatttt agggttcatg aacctactgc ctattccggc attagatgga
     1621 ggatacatta tgttcctgct ttgggaagtt attacaggcc gaaaggttaa tgacaaggta
     1681 atggagatag cgactacggc aggcatgata ctgcttatcg gattactgtt gtatgccaat
     1741 gggttggatg taattagagc attgaagtaa tggattattt tgaatggttt ggcctggata
     1801 ggaaattcaa tattgatcaa gccgggttga gaaaagcatt tctgacaaaa tcaatggaac
     1861 ttcatcctga caaaaccggt ctgaatgaaa acaaggctat ggaactgtcg gaatataaca
     1921 acaaagctta tggaatactc aaggattcct ataagacgct ggactatatt cttcacctgg
     1981 aaggcattgt tctcgctgat gaaaagtacc aattacctca agatttcctg atggaaatgc
     2041 ttgaattgaa tatggaaata gaggatgccg gagagagtgg agataaagaa aaactgaata
     2101 agatcaaaag cgatctggag acgaaaggaa ttgaagaaga tgctaacatg gaaatactta
     2161 aaaatcaata tgatagtaga agtattgatc cacaaggcta cgaaagtttg aaaaaatttt
     2221 attttattcg caaatatctc ttgcgaattg aagaaagtat taatactttt gcatcccatt
     2281 gaagctaaaa gcagtttctg agaataattt attgccgctg tggcggaatt ggtagacgcg
     2341 ctggattcaa aatcctgtgg ccgtgaggtc gtgtgggttc gattcccacc ggcggtacaa
     2401 ataaaaggag ttttcggact cctttttttg ttttaagaca catcaaaacc aacacaaacc
     2461 caccaaaagg attccttcaa taagtctcgt ctttgttgta aaatatttat cagctataga
     2521 acgatctgat aaaatccgtt agaataagga ttttagtaca ccacatcttc aatcaattcg
     2581 taatgttgat tggcacaaaa gcttccggat tagtcttatt ggaatttagg ccatgttaat
     2641 gtatattttt cattgtctgg ctgtgacctc agaaaaatct tacctttgaa aaatcattgc
     2701 cagcgtacaa aacgaaacac ttcgaagtac cttttataaa aaatcccttt cggccagaat
     2761 tacccaagat gctggatgat atcatattgt gtcaggatgt gattgacacc tgcaccatct
     2821 ctgacaagta ccgcggggat ctttttagag atatacctac taagctcttt gacagataat
     2881 gcctcttcaa ggacaggaaa tggctcattc atgatttcac ctacggagac attgtcaata
     2941 ttatccggat tggacaacag gcttgaaatg atgtcacttt cagtcaatga gccaactatt
     3001 tccccctgat catttacggt cacctgagag atatcatttt ccttcatcat ctgaaagact
     3061 tccctgagtt tgctgccttt ctgtgctgta atcagtcctg acttaggctt tctcttaacg
     3121 agatcagaaa gtcggagatc catgctgata aaaccgcgat ctctcatcca gtcatcgttg
     3181 aagatctttg caacataacg gcttccatga tcgtggaaaa taataaccac cacatcatcc
     3241 ggcctgaatc tattcttaac ctgtaacaat ccggccatag ccgatcctgc gctataccca
     3301 agtaataggc cctcttcacg agccaatctg cgtgccatca aagctccatc cttatcagtt
     3361 accttttcga aataatcgat cagattaaaa tctacattct caggcaagat atcttctcct
     3421 atcccttcgg tgatataggg ataaatctca ttttcgtcaa aaatgccggt ttccttatat
     3481 ttcttgaata cagaaccata ggtatctatt cctatgatct ctatatccgg attcttctcc
     3541 ttgagaaatt tagcggtacc gctcactgtt ccaccggtac ccactcctac aacaagcttg
     3601 gtgatcttac cctccgtttg ttcccaaatc tccgggccgg ttgtttcata gtgtgcttga
     3661 gaattggccg ggttatcata ttgattaaac caataggaat ttgggatttc ctggcttagt
     3721 cggcgagcta cagagtagta tgaacgcggg tcagcagcct caacattggt cggacagact
     3781 attacctcag ctcccatggc tttgagcata tccattttct ctttggattg tttatcactt
     3841 gtggtaaata ttgctttata ccctttgacg gctgcgacta aggccagtcc catgccggta
     3901 ttgcccgaag tacactcaat gattgtgccg ccgggcttca atttcccttc ggactcagcg
     3961 acctcaacca tttttaacgc catccgatcc ttgatcgagt gaccggggtt gaatgtctct
     4021 accttagcca aaacagtgca aggcaagtct gatgtaatct tattgatacg aaccaatggt
     4081 gtattgccaa tggtttccag gatattttta aaatacatgt gaaagaatct aaattttcgg
     4141 caaaagtagg gatttatttt caaagctgat ggcagagctt gatgtgtgtt tttattaaat
     4201 tacccaaaag aaaaattaaa aatacacgga agtaattgct gcgtggaaac catccttata
     4261 cggcacaaac aaatttaaac ttaataagcc aaacgtagaa aagctccgat caatcccagg
     4321 tccgatatca ccctttgata tatgcaattt tggcgaagaa ccgggcatcc ctggcgccta
     4381 atttgatagc aacttcagga gaaacaacca tcataacatc atctgaatag ctattggggg
     4441 atactgttcc cacaactttg gcgtacacag tagagttgaa catcggattg gttattttga
     4501 tgatagaacc tcgcggggca gtacgatgca agacaaacag accattgcta cctttgagct
     4561 ctttgtgcca gaatgcaagt ccctgctcaa aagccttagt cccttcatat gattcaaatt
     4621 tttccctatt ggaagcaggt gtcgattttg cctcctcact ggtcgtcttc tctacagcat
     4681 cctgtactgc ttcttttgca atttcacgtt ctttagcggt ggatgtcgta cccaaagcat
     4741 tgggcccggg ctttttcgta tctgttttag cattgtctgc ctttgtgccg gattttgggc
     4801 ggcctttgtc agcgacgaca ttttcattga ttttattaaa gacatcgaga gggatccagc
     4861 ccagcagaag tatttgccca agttttaaat caggggctga cattccattg atctgcatca
     4921 gcttttccgg ttttatacca aaataatttc tggaaacact atataatcct tctccgggac
     4981 ttacttcata aacaacagga gcaaagtttt cacgcttctt tggttcagac tcaaccctta
     5041 tcaaacgagc actgatagga actctgatta actcatcgat ctgtatcccc ctaaccttca
     5101 gtaccggatt gagtgtcatc aattgagatt gcttaatgcc atagaacctg cacagagaat
     5161 acatcgtttg cttcggcatt acccgatgat aaaagtatat tacacccaga ctatctcttt
     5221 gaataaatat agtatcattt ttggtcaata cggataatga gtctcccgtc gctccggcct
     5281 tttgagagaa gagtgaaata acgaagaccc atgaaacaac atataataac tttctcgatg
     5341 gcataacctt aagtgtatga gaccttttaa aggcaaatgt aagtattttt tgtaaatgaa
     5401 atatctgcct gaaaagttca ataaatcagg aaatatttta atataaaaaa gcgaataggc
     5461 cgaagaaaaa acaggaagaa tcatgaagta aaactaaaaa tgggctactc ttacaacgaa
     5521 aatatttgta gcatggcgta taatcataac aaatggatgg tacagaaacg cagggaaaac
     5581 cggattggtt atcaagcttg cctgacatac ctacaggact atttaaccaa tctttttgct
     5641 agaataagtc cttactttgg gtagttccac ggtgtagttt accgattgga caaaagaata
     5701 ctgcgatccc ggtaacaaga cttcatttac gagaaaccgg tcaataatac tcaatcaaga
     5761 catgatcacc attttacatc aaataaaatg aatccttcaa atcaccattt attttgaaaa
     5821 aagtcatcgc acgatcataa ccttcttgac cccaaatacg gatggattgc ttacccggac
     5881 cagatatacg cccggtttaa ccgaatttag gggaattgtg atcgtagttt ggccggataa
     5941 ccggttgtca tgataaacaa ttctacctga aatatcagaa acggtcactg aaacggtttc
     6001 ataaaaaatc ctttccggca attgaatatg cagttcactt tcagcaggat tcggatagac
     6061 ggtaaattca acatcctgta tcaaagtacc tttgtctgaa agagtgatcc ccggattctc
     6121 aaccttctta ttgaggtaga ccttgtattc tccttgtttc aaggtaattt gacggagaga
     6181 agtattacct ttgatgctat ctctacccat gtaattataa taccattcgt ccgtaggaaa
     6241 ggtaattgcc ttactgccat ccgtgacatc aaagtttcct ataactacca tctttaagtc
     6301 attttgatcc attacgatgt atttgaataa gccattcaag tcataatcta tctcaacgtc
     6361 atcaaacaaa tcaggaaaac tatgtttcag gtgtgttagc cccgccgtca ctgcaaacaa
     6421 ctccagtcta ttctgttg
//
LOCUS       DAOQQN010000171         6425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5140_length_6425_cov_6.031397, whole genome shotgun sequence.
ACCESSION   DAOQQN010000171 DAOQQN010000000
VERSION     DAOQQN010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5140_length_6425_cov_6.031397"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="PLG24_07465"
     CDS             <1..414
                     /locus_tag="PLG24_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HMZ24764.1"
                     /translation="KTLELRMMRQCDNAVQIASYLNNHPAVKAVYHNSIPDHPHHELA
                     GRQMKHYAPLMSFELKGGLEAVSQFMRRIRIITHAPTLGDLDTLVLHPDTSSHRNIPV
                     EVKSKMGITPGLIRLSTGIEALDDLLADLDESLNV"
     gene            complement(482..1654)
                     /locus_tag="PLG24_07470"
     CDS             complement(482..1654)
                     /locus_tag="PLG24_07470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ24765.1"
                     /translation="MKINILASRFPYPLEKGDKLRLYHQIRLLSQHHELYLFCITDHK
                     TEEKDINHMRQFCKEVKLYPITMLKRIWLVFTGIFRGLPFHVGYFYQHSLKPMIHRDL
                     SATGADLTYCQLVRMVPYIKGFNSVIWLDFMDSFSRNLYNRLEKSGWWQKIWLRWELR
                     KMIEIEKASLGLTEHLSIISEQDRASLDPERKYNIVVLPNGVDTEYFHTNEYKDEIYD
                     LCFTGNLGYEPNIRAANYLIRRIGKRLKEDITIRIAGARPGRQLRSLNSSRFVVEGWV
                     EDIRDVYWTGKLFVAPLFTGSGQQNKILEAMACGLPVITTSQVNNAIGAEVGTEIFIA
                     DNLRTFVAQIDDLLDDEDLRHEVAYQARRFVCDNYTWEIIGEKLEDFLRGTERIGG"
     gene            complement(1677..2309)
                     /gene="pdxH"
                     /locus_tag="PLG24_07475"
     CDS             complement(1677..2309)
                     /gene="pdxH"
                     /locus_tag="PLG24_07475"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HMZ24766.1"
                     /translation="MDLHSYRQQYKKAGLDRHDLPEDPVQFFQALFIKAKEGGVIEPN
                     AMTLATGNADGFIDARMVLLKEVKNGDFVFFTNYNSVKGHQLASNPFAALVFWWYAIE
                     CQVRVRGTVTKLDESESDAYFNSRPHDSRAGAIASNQSEVVADYDKMMDNFEDLRNKS
                     DAELLRPSNWGGFCLQPAEIEFWQGRPNRMHDRFLYLREGEQWRINRLSP"
     gene            2597..2983
                     /locus_tag="PLG24_07480"
     CDS             2597..2983
                     /locus_tag="PLG24_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HMZ24767.1"
                     /translation="MNLTFGSKMDNITIGRLGEDIATAFLESKGYEILERNWRWLRIE
                     VDIIVMKDNTLTVVEVKTRTSNQYGDPDYFVDDRKLAILLDAAAHYAEKISHTWSIGI
                     DIVGILLHKDGSHSLKHIEDVYFPTF"
     gene            complement(3390..4376)
                     /locus_tag="PLG24_07485"
     CDS             complement(3390..4376)
                     /locus_tag="PLG24_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HMZ24768.1"
                     /translation="MGYRKGDKVIYLPTSEAATVTRVVSNELIYIRMESDREELPAFS
                     DDVAPVVSDNRPDRKSKSETQNIAEVFYKEEIREGLYLIFQYKMGQMDADSVLPVFLY
                     NHSNQAIEFDMNYFSNNMPAQQYKGRLDPGVIDLILHLPVSSLEHQPTFEIDWNLKAD
                     PGIKKQGDIVIRPAVFFKKYGHIAIINTSGSMYYVQPVKAEKKAESLAGYTRAERKLH
                     IPKPRREQSRTVREKAEFDPVLDLHIEKLYPNPKSLKKGEILPLQLKCFDKFLENAIR
                     LKIQQVFIIHGMGNGKLKDEIATRLVLHPGVNTFKNEYHPSFGFGATEVWLY"
     gene            4575..5207
                     /gene="queE"
                     /locus_tag="PLG24_07490"
     CDS             4575..5207
                     /gene="queE"
                     /locus_tag="PLG24_07490"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HMZ24769.1"
                     /translation="MAYTIKEIYYTIQGEGRHTGRPMIFCRFAGCNLWNGREQDRMQA
                     VCKFCDTDFIGTDGANGGKYDSTALVEKILTLWPKDCGIQPAVVFTGGEPALQIDETL
                     IAEMKKSEIYIAVETNGTIPLPDGLDWICVSPKGDAVIKQKTGNELKLVYPQPENQPK
                     QFEGLDFKYFYLQPLEDEFWIQNTAAAVEYVKSHPKWRLSVQTHKYINIP"
     gene            complement(5292..6029)
                     /locus_tag="PLG24_07495"
     CDS             complement(5292..6029)
                     /locus_tag="PLG24_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HMZ24770.1"
                     /translation="MAGKVSNHHEKRFLVGPRSRLKEMLYAFDVFKEFIKGFRALHFI
                     GPCVTVFGSARFDESNEHYQKARELSASISKLGFTILTGGGPGIMEAANRGAKDAGGL
                     SVGCNIVLPHEQSTNPYLDKYVTIKYFFVRKTLLIKYSYAFIICPGGFGTLDEFFEAL
                     TLIQTGKIEKFPVVVFGSMFYNGIKEHIEKMLNEKTISQQDRDLLIFTDSIDEAINHI
                     RSKMVRNFGFRLKANPILGEKELLYNQ"
BASE COUNT         2050 a         1350 c         1145 g         1880 t
ORIGIN      
        1 aaaaccctgg agttgcgaat gatgcgacag tgcgacaacg ctgtacaaat tgcctcttac
       61 ctaaacaacc acccggctgt aaaggcagtg tatcacaatt caattccaga ccatccacat
      121 cacgagcttg ccggaagaca aatgaaacac tatgctccct tgatgagttt cgaactcaaa
      181 ggcggattag aagctgtctc ccaatttatg cgaagaatac gcatcatcac acacgcgccg
      241 acacttggag atctcgatac cttggtgtta catcctgata catcctcaca ccgaaatata
      301 cctgtcgaag taaagtctaa aatgggtatc actcccgggc tgattagatt atcaacaggt
      361 atagaggctt tggatgattt attggctgat ttggatgaat ctttgaatgt ataaattggt
      421 ctactgggca tttttattaa ttactacaaa atcatcaaat ctcgagatat cttcaatctt
      481 ctcaccctcc aatgcgttct gtgccgcgaa gaaagtcctc cagtttttca ccgataatct
      541 cccaggtata attatcacat acaaatctcc ttgcctgata ggctacttca tgtctcaagt
      601 cctcatcatc cagcaagtcg tcgatttgag caacaaacgt ccgaaggtta tctgcaatga
      661 atatttccgt acccacctcg gcaccaatgg cattatttac ctgagatgtt gtgatgacag
      721 gcagtccaca ggccatagcc tcaagtatct tattttgttg cccacttcct gtaaacaagg
      781 gtgcaacaaa caatttaccg gtccaataca catctcgaat atcctccacc catccttcca
      841 caacaaaacg cgagctattc aggctgcgta actgcctgcc cggccttgca ccggcaatgc
      901 gtatggtaat atcctccttc aacctttttc cgatacgccg aatcagataa ttagccgctc
      961 tgatgttggg ctcatagcct agattgccgg taaagcacaa gtcatagatc tcatccttat
     1021 actcattggt atgaaagtat tcagtatcga ccccatttgg cagtacaacg atattgtatt
     1081 ttctctccgg atcgagggat gctctgtctt gttcagaaat gatacttagg tgttcagtca
     1141 aacctaagga agctttctca atctcaatca tttttctcaa ttcccaacgg agccatatct
     1201 tttgccacca tcctgacttc tccagcctgt tatacagatt ccgagagaaa gaatccataa
     1261 aatccaacca gatcacacta ttaaaccctt tgatataggg taccatacgg accaattggc
     1321 aataggtcaa atcggcaccg gtagcggaca agtctcggtg tatcataggt ttgagactat
     1381 gttggtagaa atatccgaca tgaaatggaa gaccgcgaaa tattccggta aaaaccaacc
     1441 atatcctttt caacattgta atgggatata gcttcacttc cttgcaaaat tgcctcatgt
     1501 gattaatgtc cttttcctcc gtcttgtgat ctgtgataca gaacaaataa agttcatgat
     1561 gctgtgacaa cagacggatc tgatggtaga gacggagctt gtcgcctttt tccaatggat
     1621 acgggaagcg ggaagcgaga atatttatct tcaaagtatc atcttttggt aaaaaatcaa
     1681 ggactaagtc gattgatacg ccattgttcg ccttctctaa ggtataaaaa gcgatcgtgc
     1741 atacgattag gtcttccctg ccaaaattca atctctgcgg gttgcaggca gaagccgccc
     1801 cagttagaag gacgaagaag ttctgcatcg cttttgttac gcaggtcttc aaagttatcc
     1861 atcatcttat cataatcggc cacgacctca ctctgattgg aagcaattgc acctgccctg
     1921 ctgtcatgcg gcctgctgtt aaaatatgcg tctgattcag attcatcaag cttggtgact
     1981 gtgccgcgaa ctcgtacctg acactctatg gcataccacc aaaaaactaa tgcagcaaaa
     2041 ggatttgatg ctaattgatg ccctttgaca gaattatagt tagtaaaaaa gacgaaatca
     2101 ccattcttaa cctcttttaa caaaaccatc ctggcatcaa taaaaccgtc ggcattgccc
     2161 gtagcaagag tcattgcatt gggttcaatt acaccaccct ctttggcctt gatgaaaagg
     2221 gcttgaaaaa attgaaccgg atcttcaggc aaatcgtgcc tgtctaatcc tgctttctta
     2281 tactgttgcc gataactatg gagatccatt atgcttttgg gataaaagcc aaagataaaa
     2341 cagaaatcac aatttgcgaa taaagtttga agtaaaatcg gcaacttaaa acattcccag
     2401 gtacattaca aaacaatcgt agctaaaaca gaaaaaatcc gggcacctaa ccgatgtccg
     2461 gattaaacca tgaaattaaa ataaaacgat ttaagacaat taccttataa ttctgttgaa
     2521 caagtcttcc aaattacaaa caacaaaata tagacgtatc aatattaggc tatattgcct
     2581 gcaaaatgtt aattttatga atttaacatt tggttccaaa atggataata ttaccatcgg
     2641 acgattagga gaagatatcg caactgcttt ccttgaaagc aagggatatg aaattctgga
     2701 acgcaactgg aggtggctcc ggattgaagt cgacatcata gtaatgaagg acaacacact
     2761 tactgtcgta gaagtaaaga ctcgaacttc caatcaatat ggcgatcctg attattttgt
     2821 tgacgaccga aagttggcaa tattgctgga tgctgctgca cattatgcag aaaaaatcag
     2881 ccacacatgg tctattggga ttgatattgt tggcatactg ttacacaagg atggctccca
     2941 tagcttaaaa cacatagagg atgtatattt ccctacattc tgattgattc aacaattcat
     3001 ttaaatctaa tgagataacc cttcctaaag tagcctggaa tgcagcacac acatcatttg
     3061 atatgattag gaggagaggt aaagcggaac cttaaaggac atggtcagca aagggaaatt
     3121 cgaagtatgt acatgattaa taacctccca tttatgaatt accgaccatt caagatgcta
     3181 aattttattc aaactctcgt attcggcaat aaatggatat tacttcctca aattatccta
     3241 cataacaata attagggtta ttattttcct acctacttca tattgacaaa aacgctcttt
     3301 tttttcatgc cttggcaatt ctatctcctc aacattctgt gctctatatt attgataaaa
     3361 cgttttattt ctcttcgtaa tcttacagat cagtataacc atacctcggt cgctccaaag
     3421 ccaaaacttg gatgatattc atttttgaac gtgttgacgc cgggatgcag tacgagtctg
     3481 gtcgctattt catccttcaa ctttccattt cccattccat gaatgatgaa tacctgttga
     3541 atcttcaatc gtatagcatt ctccaaaaac ttatcgaaac atttcagttg caacgggagt
     3601 atttcacctt tctttaatga ctttggatta ggatacagtt tttcgatatg taagtcaagt
     3661 acgggatcaa attctgcttt ctcacgtact gtgcgcgatt gttcacgtcg gggtttagga
     3721 atgtgcaatt tcctttcagc tctggtatag ccagccaatg actctgcctt tttttcagcc
     3781 ttaactggtt gtacataata catacttccg ctggtgttga tgatagctat atggccatat
     3841 tttttgaaaa aaacagcagg cctgatgact atgtcacctt gtttcttaat acccggatca
     3901 gccttcaaat tccaatcaat ttcaaaggta ggctggtgtt ccaatgaaga aactggtaga
     3961 tgtaaaatca ggtcgataac acccggatcc agccggcctt tatattgctg agctggcata
     4021 ttattcgaaa aataattcat atcaaactca attgcttgat tggaatgatt gtatagaaat
     4081 acaggcagca cagaatctgc gtccatttga cccattttat actggaaaat aagataaagg
     4141 ccttcacgaa tttcctcttt atagaagact tctgcaatgt tttgagtttc cgacttactc
     4201 tttctatccg gcctgttatc ggaaacgaca ggagcgacat catcagaaaa agcaggcaat
     4261 tcctcacgat cactttccat acggatataa atcaattcgt tgcttacaac tcttgtaact
     4321 gttgctgcct cacttgtagg caaatagatt actttatctc ctttccggta tcccattgtg
     4381 tataaattct cttttcgctt tcaaaatgaa taatctacat tcatattgtc atttaaatta
     4441 ataattagat aaggaaagat tgttataaaa ctcccttact gcataaattt gataataaat
     4501 tgaaccaaac tcaaactact tgaacaaaaa agccagataa agagttttat ctcatcaatt
     4561 aaatatgcaa tagagtggct tatactataa aagaaatata ttatacaatt cagggggaag
     4621 gcaggcatac cggaaggccg atgatcttct gtcgcttcgc gggttgcaac ctatggaatg
     4681 ggcgtgaaca ggatcgtatg caagccgttt gcaaattttg tgatacagac tttatcggaa
     4741 cagatggtgc caatggcggg aaatacgatt ctactgcact ggtagaaaaa atcctaactc
     4801 tatggccgaa agattgcggt atccagcctg ctgtggtatt tactggcgga gaacctgcat
     4861 tgcaaatcga tgaaacattg attgcagaga tgaaaaaatc agaaatatat atagctgttg
     4921 aaacaaatgg cactatcccc ttgccagatg gattggactg gatatgtgtc agcccaaaag
     4981 gagacgctgt gatcaaacaa aagaccggca atgagttaaa attggtctat ccgcaacccg
     5041 agaatcagcc caaacaattt gaaggactgg attttaaata tttctatctt cagccccttg
     5101 aggatgaatt ttggatacaa aacactgcag ccgctgtaga atatgtaaaa tctcacccaa
     5161 aatggagact tagtgtccaa acacataagt acatcaatat tccgtagttc ctttgtaaag
     5221 gcacaatccc tatttattat aaaaatgcag tagcatgaat aataaaaata gtctatttca
     5281 ataagttcaa tttattgatt ataaagaagt tctttctcgc ctagaatagg gttagccttt
     5341 aaccgaaaac caaaattacg gaccattttt gatcggatat gattgattgc ttcatctatt
     5401 gaatccgtaa atatcaacaa atctctatcc tgttgagaga tggttttttc attgagcatc
     5461 ttttcaatgt gttctttgat accattataa aacattgaac caaacacaac aaccggaaat
     5521 ttttcaattt taccggtctg tattaaggtc aatgcttcaa aaaattcatc caatgttcca
     5581 aaaccacctg ggcatattat aaatgcatat gaatatttaa tcaataaagt ctttctgaca
     5641 aaaaaatatt taatggtgac gtatttgtca aggtaaggat tagttgattg ctcatgaggc
     5701 aaaacaatat tacatccaac agatagccct cccgcatctt tagcgccccg attggcagct
     5761 tccataatcc cggggccacc acctgtcagt attgtgaatc caagctttga aatacttgct
     5821 gagagctctc tagctttttg atagtgctca ttactttcat cgaatcgggc ggaaccaaat
     5881 actgtaacac atggcccgat aaaatgcaaa gccctgaaac ctttgataaa ttccttgaaa
     5941 acatcaaagg catataacat ttctttcaac cttgatctgg gaccaaccag aaaacgtttt
     6001 tcatgatgat tagatacttt acctgccata aaatccacca tttaattcaa aaatattgta
     6061 agataaggtt ttcctgcata gagtacaacc atacttatac aatttacttc aaaatcttcc
     6121 atctaaaaag aagagatatc aaaatatact aatgtatata ctattaacaa tctatatctt
     6181 ataaaataat aaaagaagaa agaaaggagt attattttaa cgtactattg agccagccag
     6241 taaagagaaa tgaattaaat aattgaaatt ttcaaatatt tgaatgacag cggacagtag
     6301 attcaaagaa ttactcaaca ccgcaaaata aaaaacccct tgaaattcaa ggggttaaat
     6361 gcaacttttt gtagcccgta ggggaatcga acccctcttg caagaatgaa aatcttgagt
     6421 cctaa
//
LOCUS       DAOQQN010000172         6425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5141_length_6425_cov_5.931711, whole genome shotgun sequence.
ACCESSION   DAOQQN010000172 DAOQQN010000000
VERSION     DAOQQN010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5141_length_6425_cov_5.931711"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1746
                     /locus_tag="PLG24_07500"
     CDS             <1..1746
                     /locus_tag="PLG24_07500"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMZ24771.1"
                     /translation="NDDNYLFTMTQWYPRMCVYSDFQGWQNHQFSGRAEFALTFGDFH
                     VEMTVPSDHVVCATGECQNYDKVLTPAQLERWKKAQTASDVMEIVTLDEAKAAEKKKS
                     RTSKTYIFEAKNVRDFAWGSSRKFVWDALPVDIEGKKVMAMSFYGKEAYGLYRPFSTK
                     VVAHTIRTYSHFTIPYPYPVAQSVEASNGMEYPMICFNYGRTEEDGTYSEGIKNGMIG
                     VIIHEVGHNFFPMIINSDERQWTWMDEGLNTFVEYLTEELWDNKFPSKRGPASSIVNY
                     MKLPKDELEPIMTNSDNIVSFGPNAYAKPATGLNILRETIMGRELFDHAFRNYCRKWA
                     FKHPTPADFFRSMEDASGEDLDWFWRGWFYSTDACDISLDTVKYAVADVNATVKTSRP
                     MKRSVQGPLLNDFDDISKVRNREDKNIVFETDRDTTLRDFYWKYARNLETQDTTKFNV
                     LWPESVEPLDESLKKQFEKFHAYELTLSNKGGLVMPVIIEWTYTDGSKEVERISAQIW
                     RKNENKITKSFIKEKEVASILIDPFKETADIDESNNGWNVIPQPSKFKAFKQRKTMEK
                     GEGTDINPMNVKKRT"
     gene            complement(2158..3072)
                     /locus_tag="PLG24_07505"
     CDS             complement(2158..3072)
                     /locus_tag="PLG24_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24772.1"
                     /translation="MNRFFLIIITTALFILLSTFAWAQGCSDAGFCTINSLKPNNADG
                     ITSFQNQFKIGAYYGAASNSINVYGGYLEYNRFLNNKFSIDAKLTTMAQSGNGISTFG
                     LSDFYVNSTYTASQYFFVTAGVKIPLAKSNKSKDNLPLPMDYQSSLGTFDVILGIGID
                     IKKLQVTAAFQQPLTANDNQFTTAVYPENSKFQNFQSTNQFKRKGDVLLRIAYPIRLG
                     SKLRITPSILPIYHLSNDLYTDDKNVEQEIIGSKGLTLNGNLFLDYRINDRNNIQLNG
                     GMPFIVREARPDGLTRSLIATLEYAIRF"
     gene            complement(4480..5610)
                     /gene="hemW"
                     /locus_tag="PLG24_07510"
     CDS             complement(4480..5610)
                     /gene="hemW"
                     /locus_tag="PLG24_07510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HMZ24773.1"
                     /translation="MSFSGGLYIHIPFCRQACHYCNFHFTTSLHYKSRLVEAINREIR
                     IRAAHFEKPELKTIYFGGGSPSLLSISELNTIMESIHRVFNTSGVVEITLEANPDDLT
                     PEYIRLLKTIGIDRLSIGIQSFYEEDLMWMHRAHNNIQAMNCIPMAQDIGLGDISVDL
                     IFGFPLLTDEKYMENLKKLTDWNIPHISAYSLTVEDKTPLAKSIRKGIDSRPDEGKSA
                     RQFVLTHHILEGKGYEHYEISNFSLPGWHAIHNSNYWLGVPYIGVGPAAHSFYEDCRY
                     WNIANNQKYMHLIESGQETGEYEIINPDTAYNELILTRLRTKWGVDRADILRIGTKYA
                     DHFDSIIGVLIDNGIVEVNANNYKLTFEGQMMADHWTEELFI"
     gene            complement(5610..6206)
                     /locus_tag="PLG24_07515"
     CDS             complement(5610..6206)
                     /locus_tag="PLG24_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HMZ24774.1"
                     /translation="MVEDLLVSDDVVKEHFACDLNACKGACCWEGDFGAPLDKKELKI
                     LDDIKDKVSSYLSEASNKVLAKHGAYTYFEEMEDYGTTLMPDGACVFMTTNELGIAGC
                     GIEQAYRDGVVDWPKPESCHLYPIRVTKNAEGNDYALNYDRWSICRNACSKKDAPQIR
                     LFEFVKDGLIRRYGKEFYEQLDYLAGVVNGPDVPESNG"
BASE COUNT         1941 a         1169 c         1323 g         1992 t
ORIGIN      
        1 aatgatgata attatttatt taccatgaca caatggtatc ccagaatgtg cgtttatagt
       61 gactttcagg ggtggcaaaa tcaccagttt tccggaagag cagaatttgc actaaccttc
      121 ggggattttc atgtggaaat gactgtccca tcagatcatg ttgtttgcgc aaccggggaa
      181 tgtcaaaatt atgacaaggt ccttactcca gcacaacttg aacgttggaa gaaagcccag
      241 actgccagtg atgtgatgga gattgttaca ttggacgaag ctaaagccgc agagaagaag
      301 aaatccagaa catccaagac atacatcttt gaggctaaga atgtccggga ttttgcatgg
      361 ggttcatcca gaaagtttgt ttgggatgcc ctcccggtag atatagaggg aaaaaaggta
      421 atggcaatga gtttctatgg taaggaagcc tatggcttat atcgaccttt ttcaaccaag
      481 gtagtggcgc ataccattcg cacttattcc cattttacga ttccttaccc ttatccggtt
      541 gctcaaagtg ttgaagccag caacggtatg gaatatccga tgatatgctt caactatggg
      601 cgtactgaag aagatggaac ttattcggaa gggataaaaa atgggatgat tggtgtcatt
      661 atccatgaag taggccataa tttcttcccc atgataatca atagcgatga gcgccaatgg
      721 acttggatgg atgaaggatt aaatactttt gttgaatatc tcactgaaga attatgggat
      781 aacaaatttc catccaaacg cggacctgct tccagcatcg ttaactatat gaaattgccg
      841 aaggacgagc tggaacccat catgaccaat tctgacaata tagtgtcctt tggtccaaat
      901 gcctatgcaa aaccggctac agggttgaat atcctgagag aaaccattat ggggcgggaa
      961 ttgtttgatc atgcctttcg gaactattgt cgtaaatggg cattcaagca tccaacaccg
     1021 gcagattttt tcaggtcgat ggaagatgcc agtggcgaag atcttgattg gttctggcga
     1081 ggctggtttt atagtaccga tgcatgcgat atctcattgg atacggtgaa gtatgctgtt
     1141 gccgatgtta atgccactgt taagacatcc aggcctatga agcgatcagt gcagggtcca
     1201 ttgctcaatg attttgatga tatatctaaa gtgaggaatc gggaagataa aaacatagtt
     1261 ttcgaaacag atcgggatac gaccttaaga gacttttatt ggaaatacgc ccgtaatctg
     1321 gaaactcaag atacaacgaa gtttaatgtg ctctggccgg aaagtgttga acctttggat
     1381 gagagtttga agaagcagtt tgaaaagttt catgcctatg aattgacttt gtccaacaag
     1441 ggaggcttgg tcatgcctgt catcatagag tggacttata cggatggaag taaagaagtc
     1501 gaacgcattt cagcccagat atggcggaaa aatgaaaata aaattaccaa atcattcatt
     1561 aaagaaaaag aagtagcttc tattttgatc gatccattta aggagactgc agatatcgac
     1621 gaatccaaca atggatggaa tgtcatacct caaccatcca aatttaaagc gtttaagcaa
     1681 agaaagacca tggagaaagg tgaagggaca gatatcaatc caatgaatgt taagaaaagg
     1741 acttaattgg ttttaggtaa gtgtgaatag taaagccggg agcatttttt ccggcttttt
     1801 atattatcca atagtatgat ttaaataccc cgattagctt atgtctttaa ttataaacac
     1861 tatttctccg ggataggagc aaaaattaag aagagattta tcagtaatga attctcatct
     1921 ttgatttata agttgtaatg gaacataact attaagatag atgttattct tggcattccc
     1981 aatttgtacg ataaagacat tcatttttcc ggatattttt tcaaaatacc tgcattcttt
     2041 ttttgtacga aattcagaca aaattttcaa agccatggta ataacgaatg ttccttcggc
     2101 attaacccaa aaatttatga actattatat aaatcatctt ggtataacac gtgtagatca
     2161 aaatctgatc gcatattcca aagtcgctat caaacttcgc gtcaggccat ccggtcgggc
     2221 ttccctgaca atgaaaggca tccccccatt aagttggata ttgtttctgt cattgatcct
     2281 ataatccaga aacagatttc catttaaggt cagacctttc gaaccaatga tctcttgttc
     2341 tacattctta tcatccgtgt acaagtcatt tgacagatgg taaataggta gaatacttgg
     2401 ggtgatcctt aacttggaac ctaaacggat ggggtaggct attctcaata atacatctcc
     2461 ttttctttta aattgattgg ttgactgaaa attttggaat ttggaatttt caggatatac
     2521 agctgtcgtg aattgattat cattagctgt caatggttgt tggaacgctg ctgtgacctg
     2581 gagtttcttg atatctattc caatgcctaa aataacatca aaggtgccca gactggactg
     2641 ataatccata ggcaggggaa ggttgtcttt tgatttgttg gatttagcca ggggtatctt
     2701 aacacctgcc gttacaaaga aatactgact tgctgtataa gtggaattta cataaaaatc
     2761 agataggcca aaagtagaga taccattgcc gctttgtgcc atagtagtca attttgcatc
     2821 gatactaaat ttgttgttta gaaatctatt atactccaaa tagccgccat aaacattgat
     2881 ggagttactt gccgcgccat agtaagcgcc aatcttgaat tggttttgga acgaggtgat
     2941 tccatcggca ttatttggtt ttaaactatt gatggtgcaa aatccggcat cactacaacc
     3001 ctgagcccat gcgaaagttg acaataaaat aaaaagagca gtcgtaatga tgatgagaaa
     3061 gaatctattc attttcctat atttttttaa tgatgcaatg taccttcttg ccaataatta
     3121 ttgtaagagt cttagattca atttttgaaa atatgtattt gtaacttact ggttgtcaat
     3181 aagtaaattg tagttttgtc catttgcttc atcagatttt caagatttta ataattgaaa
     3241 atatagcaaa ccctgtctat taactttatt atctgaaata tccgggacat ctttcaggta
     3301 gcttgtatag gtcgaccgat tgttgaataa aaatttgcag gatattaaat gcttgccctc
     3361 aaaatgagaa aaatagggtg taaaaaagct actaatagta ccatcaattt tctgattcac
     3421 tctattgatt tggcaataat ccaatctttg gaaagtcgta aaaagaatag gagaatttga
     3481 atattcaact attttgggcg caatgtctat acgcagaatt tcaaaatccc gctgccagct
     3541 caataagtgt gttacattat ttaccgggcc gcattctgtc tcaataagtt gtacattcgg
     3601 ctgagtggtt gaataataca catagtcata tatataagga tttaaaacca ttgtcatcta
     3661 catcaattga tattcaaaag ttactttttc ccatgtatcg gatgtccggt gtgtttttaa
     3721 taaccactta ttctgaaaaa tatactgcaa cattatgtaa atattgtgag ttgttcaaaa
     3781 gtaaacattg acaatgattt atccaacatc cggtgagaaa atttatttca actcatttat
     3841 gggaaaggat gagcttttga ttgatttttt caaaattctt ttgtggaatg aataccggtt
     3901 aatcccctga aacatttgtt tgtcctactt tctttgttga gtttaatcag tacagatatt
     3961 tcaaacatca gtacatttat ttttgtcaat cataagtggt gtatcttaca tttttgattt
     4021 tactgtctta taataaatga tcctattcat ttggattcag tatataaatg gccatgaatc
     4081 aagctgtaaa aaggacattg gatgaagtta aggatgaaag aattgtggga atgtctttcc
     4141 gctatttaaa aaacctgaga acaagtaaaa cggctaatat ttatggcttt atggagaaaa
     4201 cgaagctcga aataatttca catatgtgat gcccggatca aggttgaagg aaatcagcta
     4261 agcaaaattg acgttaacgg gaggatgcca aagccgttat ataggttgac gaatgatttg
     4321 aagtttatat catgcataac gtgaacgact catttggtgg gaggtgatgt ttcattattc
     4381 agaaaggggt tggatagtca cgtggagatc ctgattgtcg ggcgcctgag ctatttgaat
     4441 cattaattga atgcttctaa tgtggaatgt catgtttctc taaataaaca attcttctgt
     4501 ccagtgatca gccatcattt ggccctcaaa agtcagctta taattatttg cattgacttc
     4561 cactattcca ttgtcaatca gcaccccaat gatactgtca aaatggtctg catattttgt
     4621 gccaattcgc aggatgtctg cacgatcgac accccatttt gttcggaggc gtgtcaggat
     4681 caattcattg tatgcggtat ccgggtttat tatttcatat tcaccggttt cctgaccgga
     4741 ttctatcagg tgcatatatt tttggttatt ggcgatattc caatatcggc agtcttcata
     4801 gaaggaatga gctgccggcc caacacctat atagggcaca ccgagccaat agttgctgtt
     4861 gtgaatcgca tgccatccgg gtagtgaaaa attggatatt tcgtagtgtt cataaccttt
     4921 accttccaag atatgatgcg tgagtacaaa ctgtcgagca gatttgcctt cgtccggtct
     4981 tgaatctata ccttttcgaa tgcttttggc caggggtgtt ttatcctcta ccgttagact
     5041 gtaagccgaa atgtgaggaa tgttccaatc ggttagtttt ttaagatttt ccatatattt
     5101 ttcatccgta agcaacggaa agccaaagat gaggtcaaca gaaatatccc ccaggccgat
     5161 atcctgagcc ataggtatgc aattcattgc ctgaatatta ttgtgggcac ggtgcatcca
     5221 catcaagtct tcttcataaa aggattggat gccaatactc aggcgatcga tgccaatcgt
     5281 ttttaataga cggatatatt caggggtaag atcgtccgga ttggcttcca atgtgatttc
     5341 cactacacct gaggtattga atacgcgatg aatggattcc ataattgtgt tgagctctga
     5401 aatactgagt agggatggac tgccaccgcc aaagtagatg gttttcaatt ccggtttttc
     5461 aaagtgagct gcccgtatcc ttatttccct attgattgct tccaccagtc gggacttgta
     5521 atgaagggaa gtggtaaaat gaaaattaca ataatgacat gcttgcctgc aaaatggaat
     5581 atgaatgtat agccctccgg aaaaagacat taaccattac tttcaggaac atccggcccg
     5641 ttgactacgc ctgccagata atccaactgt tcataaaact cctttccgta tcttcgtatc
     5701 aaaccatcct tgacaaactc aaataacctt atctgtgggg catctttttt gctgcatgcg
     5761 tttcgacata tggaccatcg atcatagttg agtgcatagt cgttaccttc agcatttttc
     5821 gtcacccgga tgggatataa gtggcagctt tccggtttgg gccaatctac cacaccatcg
     5881 cgataagctt gttcgatacc gcacccggcg atgcctagtt cattcgttgt cataaagaca
     5941 catgctccgt caggcattag ggtcgtacca taatcctcca tttcttcaaa ataggtataa
     6001 gccccatgtt tagctaacac tttattggac gcttctgaaa ggtaagagga tactttgtcc
     6061 ttaatatcgt ccaatatctt aagttctttt ttgtccaaag gcgctccaaa gtcaccttcc
     6121 cagcagcaag ctcccttgca tgcattaagg tcgcatgcaa aatgctcctt gactacatca
     6181 tcgctcacca gtaggtcttc caccattaac ataattcaat caggatattt cgacaaaggt
     6241 aatgataatt gtgttgcgtg ctgaaaagaa aaaaggaagg aactgtactt tgaaatattt
     6301 gaatcagtgt tttatatgtt ggctggttgt ataatcagct atctctatac agggattgaa
     6361 gggtatggtg aaaaagtgag gggtgcatga gttggcggag ttttaaaatc gaagtaggta
     6421 cgaaa
//
LOCUS       DAOQQN010000173         6396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5181_length_6396_cov_5.664879, whole genome shotgun sequence.
ACCESSION   DAOQQN010000173 DAOQQN010000000
VERSION     DAOQQN010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6396
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5181_length_6396_cov_5.664879"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(416..901)
                     /locus_tag="PLG24_07520"
     CDS             complement(416..901)
                     /locus_tag="PLG24_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24775.1"
                     /translation="MKNLLLSMVILGFLVLGCRNSPSSSSDGGPVAISLPETEVDSVQ
                     VILDGNYCYRYMIHRDTYDIHLTLSQGQVRGEMNFDKQEDQNTSGSIEGYIKEDVMNL
                     KYRHRIDGTPKVRELSFRIRGNLLISAEGEELTEGDSTFFRDPASVRYEGLVYSRIDC
                     P"
     assembly_gap    1293..1392
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1673..3055
                     /locus_tag="PLG24_07525"
     CDS             1673..3055
                     /locus_tag="PLG24_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24776.1"
                     /translation="MSRFQTFSASLFPGEVMILSRHSGMSDPEKLDILKRLLHSAGNP
                     EETFEVKPGTDKRKLSYVKTWCIDKLKAVDVDVRLSRLLQWEQQILLDNIGSDEEKTF
                     LQTIRKTNASDFNFIKCYEIAHRYRHYLQVRFRKKEVATVVDFLDTFHRDYEKSKQLS
                     DEIHVATRDILILYEKKQELNTRWIGILSDIFFDEGNDGYTRILAWIRLVLIANSSND
                     YSFLEEHFKLFEAKLLDGTFYSKRIMANFYSQYLLFYANRQDFKKAAYYGRLSIKLKN
                     NDYLYYANNLVAVLLRDKNTAEALDILKSTATIASESTNFHNRLTHISYFIRALTDSG
                     KAVHAESRGFVFYNLFKKEIFNYRWYLFFSVWLRAMAYCGKFAGLLKMYKLYPGIGAE
                     EMRQNKRQGGFPFITFMSHVARYRLNEISLSELKHTAKNLGLQGGMNVPDDVADFIRV
                     VLKKEWSEGI"
     gene            complement(3131..5551)
                     /locus_tag="PLG24_07530"
     CDS             complement(3131..5551)
                     /locus_tag="PLG24_07530"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HMZ24777.1"
                     /translation="MANDNNKHTIYLPLEDVDSEHCAMIVDKGLEGVQGVASHRVELN
                     NKRAVITADNPEVVAEAVRAIKGLGYGVTTVRRSFPVLNMSCASCAVSAESIMKSTEG
                     VVDASVNFANGLLTVEYLPNMTNPEALQKIQQSVGYDLLLSEESSQQDTLEELHNKKF
                     RRLRTKTIWAIALAIPLVIIGMFFMHMSYANEIMWLLATPIVVWPGKDFYINAWKQAR
                     HRSANMDTLVALSTGIAYLFSVFNMLFPQFWLSRGMHVHVYFEAAGVIIAFILLGKML
                     EEKAKGNTSAAIKKLIGLQPKTVILVDENGNERHIAIDEVLEGDILLVKPGEKIAVDG
                     VVAFGESYVDESMLSGEPIPVLKNPGEKVFAGTINQKGSFRFKAEKVGKDTMLAQIIK
                     MVQDAQGSKAPVQKLVDRIAAVFVPVVIGIALLTFIIWLILGSENALVHGLLAAVSVL
                     VIACPCALGLATPTAIMVGVGKGAEKGILIKDAESLELAKKVDAIILDKTGTITEGRP
                     EVTDIAWSNENEETRQVLFSLERKSEHPLADAVAKFLAGTTAVDLSEFESITGKGVRG
                     VYNGKSWLAGNRGLLLDHAIAIPDDLANAAEQWGSQSKTVIWFADDVRALAVLAITDR
                     IKDSSPEAIQKLQQMGIEVHMLTGDNQSTAKAIAAQVGIHEYRAEMLPRHKADYVKDL
                     QSKGKVVAMVGDGINDSTAMATADVSIAMGQGSDIAMDVAKMTIISSDLKKIPLAIHL
                     SKQTVATIKQNLFWAFIYNIIGIPIAAGVLYPINGFLLNPMIAGAAMAMSSVSVVSNS
                     LRLKWKVD"
     gene            5849..>6396
                     /locus_tag="PLG24_07535"
     CDS             5849..>6396
                     /locus_tag="PLG24_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24778.1"
                     /translation="MIRNLLLISFTYFFFLAGVSSQTTLIDFEDATKTVYASGTVTLS
                     GHSFDMTDALIGTSASDRKNGNQSARIRAQGSITQLDNFTTGIGNVDVYHAKYGTDGN
                     STWKLSYSTDNGSTWTQAGSDVTTSGTTLVKQTFTINQSGNVRIKIEKLTTNASNNRT
                     NIDDITVYSYSSPCTPPSTYPTG"
BASE COUNT         1778 a         1508 c         1236 g         1774 t
ORIGIN      
        1 gataagatac aattcctcga tgacgtatat atcattggga atattctgac cgaaaaggat
       61 ggaatcatac tggaaacttc tggcggcaat ttccatcgac taaaagccca ggggtggcag
      121 catttcaatc agtgattctg actgaaacct tgaaaagacc cattcgaggc agcttggtaa
      181 gccaatcagt cactaatagg atgtttttta gttgtcttca aactatatta aaatatcttc
      241 gaccgttagt atgcattcgc aaaataatta cccaatctgt acaaaactgt ttgtctattt
      301 gttacaaacc cggaagcttt agttaagata taaaaactgc ttttgtacat cccggctatc
      361 gcaaacggac cttcatcatg taacccaaaa cgggaatctc cattaatcaa ccgactcaag
      421 gacaatcgat cctggaatat accaaccctt cataacgtac tgatgcagga tctctgaaaa
      481 aagtcgaatc gccttcggtc aattcttccc cttcagcgct gataagaagg ttaccacgga
      541 tcctaaagga cagctccctg actttaggtg tcccgtcaat cctatgtcgg tatttaaggt
      601 tcatgacatc ttccttgata taaccttcaa tgctcccact tgtattttgg tcttcctgtt
      661 tatcgaaatt catctccccc cttacttgac cctgtgacaa ggtaagatga atgtcgtacg
      721 tgtctcggtg tatcatataa cgatagcagt aattgccatc cagaattacc tgtacactgt
      781 ccacttccgt ttccggaaga ctgatggcaa caggccctcc atctgaagac gaagaaggac
      841 tattccggca tcccaatacc aaaaaaccaa gtatcaccat agacaaaagt agattcttca
      901 taaaaatatt actttttgaa aataaaaaat aggtattgct atctgcgaat ataacgctaa
      961 atctcacttt aatacttcaa taaatattca gaaaaaatag cctcctacca tcctgattta
     1021 cagcacattg taaagaaaag acggttgggg cgtggttttt cctgaagtag gtagatgatt
     1081 aatagtcacc ctgccaatag atatgctcct cttcgggtgc cattacgtaa attctattca
     1141 ggattgacca tattttttat tcgtccgggg caaatggttc actttacctt ggtcagcggt
     1201 tattaaacat gtacatactt ctttttttcc aaaaaaatta accgaacacc cactactgta
     1261 acgcactcct gtagtaggta gatgattaat agnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnttaaccga acacccacta ctgtaacgca ctcctgtagt aggtagatga
     1441 ttaatagaca gaaagaaagc gaggccgtac ttttaatatt ccaaacagaa aaattatctt
     1501 catatagatg attgtagttt tccgccttcc tccaaatcct ttgctatacg atccttttca
     1561 gctattactt tcgtacacac ttcgattttt ccccacaaaa caacctctct caaattttta
     1621 tttattgaac cactatctat gttttttcat cctttcacct tattttcggc gtatgtccag
     1681 atttcaaaca ttttcagctt ctctgttccc gggtgaggtc atgattctca gcagacattc
     1741 aggtatgtca gatccggaaa aattagacat cctgaaaaga ttactccata gtgccggcaa
     1801 tccggaagaa accttcgaag taaagccggg tacagacaaa cggaagctaa gctatgttaa
     1861 aacctggtgc atcgacaaac tgaaagccgt cgacgtggat gtacgcctaa gtcgcctgct
     1921 ccaatgggaa caacaaatcc tgctggacaa tattggaagt gatgaggaaa aaacctttct
     1981 acaaaccatt agaaaaacca atgcttcgga tttcaatttt attaaatgtt atgagattgc
     2041 gcaccgttat agacattacc ttcaggtgcg gttcaggaaa aaggaggtcg ctactgtggt
     2101 tgattttctg gacacatttc acagagatta cgaaaaaagc aagcaactga gtgatgaaat
     2161 tcatgttgct acacgggata tcctgattct atatgagaaa aaacaggaac ttaacacccg
     2221 gtggattggc attctaagcg atattttctt cgatgagggc aatgatggct atacgaggat
     2281 tctggcatgg atcagactgg tgctgatcgc caacagcagc aatgactatt ctttcctgga
     2341 agagcatttc aagctgtttg aagcaaaatt gttggatggc actttctact caaagcgtat
     2401 catggccaat ttctacagcc agtacctgct tttctatgcc aataggcagg attttaaaaa
     2461 ggcagcctat tatggtcgtt tgtcgattaa actcaagaac aacgactatc tttattatgc
     2521 caataacctg gttgctgtat tactgcgcga taaaaatacc gctgaagctc ttgacattct
     2581 caaatccacc gcaacgattg cctccgaatc gaccaatttt cacaatcggc ttacccacat
     2641 ctcttatttc attcgcgccc ttaccgactc cggaaaggct gtacatgcag agagccgtgg
     2701 cttcgtcttc tataatcttt tcaaaaagga aattttcaat taccgatggt acctgttctt
     2761 cagtgtttgg ttaagggcaa tggcgtattg cggcaaattt gcaggcctgt tgaagatgta
     2821 caaactttat ccgggcatag gtgcggaaga aatgcgacag aacaaacgac aaggaggatt
     2881 cccttttatt acgttcatga gccacgttgc gcgctaccgg ctcaatgaaa tcagcctctc
     2941 cgaactgaaa cacacggcaa aaaacctggg gttacaaggt ggaatgaacg tcccggatga
     3001 tgtagctgat tttattcgtg tcgtgttgaa aaaggaatgg agcgaaggta tataataatt
     3061 tccttacctc taaccccggt aaaaaacctt cgtggcaatt ccttgcctac ccgccttttc
     3121 taccctcaac tcaatccact ttccacttaa gccgcaggct gttgctgact acactgacac
     3181 tgctcatggc catggcagct cccgctatca tcgggttgag caagaaaccg ttgatggggt
     3241 atagaacacc tgccgcaatc ggaataccta tgatgttgta gatgaaggcc cagaaaagat
     3301 tctgcttgat cgttgcaaca gtctgcttgg acaaatgaat agccaatgga attttcttca
     3361 gatcggatga tataattgtc atcttggcga catccatagc aatgtcgctg ccttgaccca
     3421 tggcgatact gacatcggct gttgccatcg ccgtactatc attgatgcca tcgcctacca
     3481 ttgctacgac cttgcccttg ctttgcagat ccttgacata atcagcctta tgccggggca
     3541 gcatttctgc ccggtattcg tgaataccga cttgagcggc gattgccttg gcagtcgatt
     3601 ggttatcccc ggtcaacata tgcacctcaa tacccatttg ttgcaacttt tggatggctt
     3661 caggcgaact gtcctttatc ctgtcagtaa tagcaagcac tgcaagagcc ctgacatcat
     3721 cggcaaacca aatgaccgtt ttggattggc tgccccattg ctctgcagca ttggccaagt
     3781 cgtcaggaat tgcaatggca tgatcaagga gaaggcctct gttacctgcc aaccagcttt
     3841 ttccgttata tacaccacgc acacccttgc cggtgatact ttcaaactca ctcaagtcca
     3901 cagcggtagt gcctgcaagg aatttggcaa ctgcatctgc caaaggatgt tcggacttgc
     3961 gttccaggct gaagagtacc tgccttgttt cttcattttc atttgaccag gctatatcgg
     4021 tcacctccgg tcgtccttca gtgatggtac ctgtcttatc caggataatg gcatccactt
     4081 ttttcgccaa ttcgagactt tcggcatcct tgataaggat acctttttca gcaccctttc
     4141 cgacacccac cataatggct gtaggtgtgg caaggcccaa tgcacacggg caggcaatta
     4201 caagcacgga cactgcagcc agcaaaccgt gtacaagggc gttttcactg cccaatatca
     4261 accagataat aaaggtaagt agtgcaatgc cgatcaccac aggtacaaag accgccgcaa
     4321 tcctgtcaac cagtttctga accggcgcct tacttccttg tgcatcctga accatcttga
     4381 tgatctgagc cagcatcgta tccttgccta ctttttctgc tttgaatcgg aagctgcctt
     4441 tctgattgat ggtcccggca aagacctttt ctcccggatt cttcaatacc ggtattggct
     4501 caccactgag catactctca tccacatagg attcaccgaa agccacaaca ccatcaacag
     4561 caattttttc accgggtttt accaataaaa tatcgccttc caggacttca tcaatggcaa
     4621 tatgacgctc attgccattc tcatcaacca gaattaccgt ctttggctga agaccgatca
     4681 atttcttgat tgctgcggaa gtattgccct tggctttttc ttctaacatc tttcccagaa
     4741 ggataaatgc aatgataacc ccggcagcct cgaaatatac atgtacatgc atgccacgac
     4801 taagccagaa ttgtggaaaa agcatattga atacgctgaa cagataggcg attcccgtac
     4861 tcaaggctac cagagtatcc atgttggccg accgatgacg cgcctgtttc caggcattga
     4921 tgtaaaagtc tttgccgggc cacacaacga tcggagtcgc cagtagccac attatctcat
     4981 tggcatagga catatgcatg aaaaacatac caataataac cagaggaatg gctagcgcta
     5041 tcgcccagat agtttttgtc ctaagccttc tgaatttttt attgtgaagc tcctccagtg
     5101 tatcttgttg agaagattcc tcactgagca acaggtcgta gccgacagac tgctggatct
     5161 tttgcaaagc ctcaggattg gtcatgttag gcagatattc gaccgtgagc aacccattgg
     5221 cgaagttgac cgaggcatct accacacctt cagtggactt catgatggat tcagcactaa
     5281 cggcacatga ggcacaagac atattgagca ccggaaaaga acgtcggact gtagtgactc
     5341 catatccgag gcctttgata gccctcaccg cctcggctac cacttcagga ttgtcggctg
     5401 ttatgaccgc ccttttattg ttcagctcca ccctgtgact tgcaacacct tgtactcctt
     5461 cgagaccctt gtctacgatc atggcacagt gctcactgtc cacatcctcc aaaggcagat
     5521 atatcgtatg cttgttatta tcgttagcca tataatgatt tcagaataaa gaaatgtgta
     5581 atcgtccttt caggcagcct ttatcaggca tgtatgaggg aatttggcaa tgattaaacc
     5641 attggcagca tcaaatggtt cagcggataa ggcaaaccca taaacctcag tcatctctag
     5701 cgttccataa tttaaccatt tcttatcatc ttatcgctct tacatcttcg caaggattat
     5761 tatctttgca cccgaactac tagcaagttg cttcgaccgg tccggtcatg ctactatatt
     5821 ttttcattct tttttaaatt attattctat gattagaaat ttactcttaa tttcatttac
     5881 ttacttcttt tttttagccg gagtctcatc acagacaacc ctgatagatt ttgaagatgc
     5941 gacaaaaacc gtttatgctt caggtactgt cacgttatcg ggacattcct ttgatatgac
     6001 tgatgctttg ataggaactt cagctagtga cagaaaaaac ggaaatcaat ctgcccgtat
     6061 cagggctcaa ggatctataa cccagttaga taattttacg accggcattg gcaatgtgga
     6121 tgtttatcat gcaaaatatg gaacggatgg taattctacc tggaagttga gctattccac
     6181 tgacaatgga agcacctgga cgcaagcggg aagtgatgtc accacgagcg gtacaacatt
     6241 agtcaagcag acatttacca ttaatcaatc cgggaatgtc agaatcaaga ttgaaaagct
     6301 aactacaaat gcaagtaaca acagaaccaa tatcgatgat ataacggttt attcatattc
     6361 ttcaccctgt actccaccat ctacctatcc cacagg
//
LOCUS       DAOQQN010000174         6379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5204_length_6379_cov_5.051866, whole genome shotgun sequence.
ACCESSION   DAOQQN010000174 DAOQQN010000000
VERSION     DAOQQN010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6379
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5204_length_6379_cov_5.051866"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(298..3798)
                     /locus_tag="PLG24_07540"
     CDS             complement(298..3798)
                     /locus_tag="PLG24_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24779.1"
                     /translation="MLIRFRKYVSYIIPALLLSISAKAQVMGPLGNLRSIFFHPDSLP
                     LSIPETPVPGTWSFYLQDRSTLVLNDFFEISNNIVRRRIPLPDSLSMETVVLSYRIFS
                     KNLSLPYRLMDTSMILQVWDPTLSERELRYASSPLIPERNTMNYRGAFTRGISLGNTQ
                     SLALNSAFNMQLSGELGDDIKIVGALSDNSIPIQPEGNSQQLQEFDKIYIVLSRKNSS
                     LTVGDFELNRPPGYFLNYFKKLQGVQASTTATLKGNKLSTSGGIAVTKGKFNRFTLTV
                     TEGNQGPYRLPGAEGEAFVIVLAGTERVYLDGIQLVRGFDRDYIIDYNSGEITFTPTR
                     IITKDSRIVVEYEYSNQQYLRSFANLNVRYQTKKWDFNLNAYNERDSKNSDRVRELSA
                     EDRMTLQQAGDSISNKLVNSIREIDPSNTSVVVSYQLNDTIINNQPFSFLVFPWKEGS
                     KRYLADFTDVGQNNGYYDRVNDGINGRVYRFAGIDADGRPKGRYNPVSRLTAPAQRQM
                     FTAGAAWKPDTNTVIRLETALSGQDNNLFSSEDDADNYGLGVHVYAERKNILVSDKRK
                     WTASVGGEYEHTGLQFNPITPYRSQEFNRDWNIASIPLESQQYLRLFLNQNIGTQLRL
                     NYALSGLFQPIHFKGVKQEAALNYKLKKFEFRADASYLKSESINETSAFLRPNVSIRR
                     AFPRLGGLNLIATYQGEYNRRRSVSSDTLLATSFAFHVMRLSAQNTFSKNFSSEIYIQ
                     RRNDYLPFTHDLLLANHALEYGIKSTLKSGLSSVLNLTATVRTFDADRIKPGAKFNSK
                     DTYLGQLDYLFSALKNRLSGNTLFEVSSGQEQKTEFNYLAVQPGTGQYVWNDYNNDSI
                     QQVNEFTTEGFPEMRNYVRVNILTNQFIPTLNTSFNQSFRWQGKSTGKNKTARFFNRF
                     SSQTGYRFDRKIKDTTKKGFWNPFYTNIPDTSLVTYLQNFRHTFFYNRGDSRYEIQLN
                     ANTNNVRNVQITGYERRRTDVYGLVIRYSLGAPYTLLFNYNRSHRFSDSEAEAFKLNN
                     FSIDADEIRPEFNWIIASDYRISLNYRFLKQKNLLAPHYEKAASNDIKLNFDFNQNSL
                     SNLRLSASISMIRFDGIRNSAVEFAMLEGLKNGRNYLWSVNYDLNLKNNVQLSFGYDG
                     RKSEGINVVHTGNASIKAYF"
     gene            complement(4015..5073)
                     /gene="selD"
                     /locus_tag="PLG24_07545"
     CDS             complement(4015..5073)
                     /gene="selD"
                     /locus_tag="PLG24_07545"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HMZ24780.1"
                     /translation="MTEEKIRLTKYSHGGGCGCKIAPQVLDEILKGSVSGITSGKLIV
                     GNGSRDDAAVYRLNDNEALISTTDFFTPIVDDPFIFGKVAAANAISDVYAMGGDPIMA
                     LAILGWPIDKLPVEAARAVLDGAITICNEAGIPLAGGHSIDTPEPIFGLAVSGRVSMR
                     HLKRNDTAREGDLLLLTKPLGVGILSTAGKRGAITEEDLEILFSQLTRLNSVGGQLGK
                     LDSVHAMTDVTGFGLGGHLVEMVEGSGLSAVLEYSKVPILAGVHQYLAQNIMPDATYR
                     NWNGYGAKIELSPEINIMDAFRILPDPQTNGGLLIAVSPDGLEAAREILTDNGYDAFT
                     EPIGRITAKGEKAITVLA"
     gene            complement(5070..6110)
                     /gene="mnmH"
                     /locus_tag="PLG24_07550"
     CDS             complement(5070..6110)
                     /gene="mnmH"
                     /locus_tag="PLG24_07550"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HMZ24781.1"
                     /translation="MAVQQIDIEQFLQLAEEHPVFDVRSPSEYGHAHIPGALPLPLFS
                     DDERREVGTTYKQRSREAAIKIGLDYFGIKMRQMVCLVEDILHHKGTNTVLLHCWRGG
                     MRSGAVSWLLDMYGFRVFSLRGGYKKYRNWVLDQFSRPYSLRILSGFTGSGKTEILLE
                     MARLGVPTVDLEGLAGHRGSAFGALGMPPQPTVEQFENKLAQELYTISREHPGKTIWL
                     ESESNRIGCVNIFHTFFNQMKAAPRIHTEVSHEARLGYITAGYGQFSTEKLKDAVLRI
                     QKRLGGLETKNALAHLDAGCIRDAFDILLKYYDKFYASGGIYQPAIMTIELPGTNAYE
                     NARLILQKTKDL"
     gene            complement(6177..>6379)
                     /locus_tag="PLG24_07555"
     CDS             complement(6177..>6379)
                     /locus_tag="PLG24_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24782.1"
                     /translation="YFTNGQGFSYQSPDHIGKSISQSINHLPTSVALVICHIFDLGRP
                     LRLVYQHMQWNIPAIAGETVFL"
BASE COUNT         1735 a         1470 c         1415 g         1759 t
ORIGIN      
        1 ttctttgacc gttttttaac ataatttgtt gaataatgcg caacttgggt taatgctcat
       61 aatcgtggga ggcctttcta tgactatctt tgaagccaat caaaggttat gtataaaaaa
      121 gttattttat tgtggaattc caatcactaa aaaaatggat ttgtcaatgt cattcaatgt
      181 tatatatgcg gagctgatag acggcagttt ttaagaggtg aaaaaagaaa aaaagaatct
      241 ttaggggagc gacatatcaa gcctcaaagc aacgatcaaa gtgctcaccg ggtgccctca
      301 aaaatatgcc ttgatgcttg cattgccggt atgcaccaca ttgattcctt ctgacttcct
      361 gccatcgtaa ccaaagctca actgcacatt gtttttcaaa ttgagatcgt agttgaccga
      421 ccaaagatag ttgcgtccgt ttttcaagcc ctcgagcatc gcaaattcaa cggcagagtt
      481 tctgattcca tcaaacctga tcatcgaaat agaagcggaa agccggaggt tggataaaga
      541 gttctgattg aagtcaaaat ttagcttaat atcattggaa gcggcttttt catagtgggg
      601 tgcaaggaga ttcttttgtt tcaggaagcg atagttcagt gaaatacgat aatcggatgc
      661 aatgatccag ttgaattcgg gccgaatttc atcagcgtca atcgaaaaat tgttgagctt
      721 gaatgcctcc gcctccgaat cactaaaccg gtgcgacctg ttgtaattga acaacagggt
      781 atatggtgca cccaatgaat atctaatcac caatccatac acatccgtcc ttcgtcgctc
      841 gtatcctgtt atctgtacat tccgaacatt gttagtattg gcattcaact gtatctcata
      901 acgcgaatca ccccgattgt aaaaaaaggt atgtctgaag ttctggagat aggtcaccag
      961 tgatgtatcg ggtatatttg tgtagaatgg attccagaat ccctttttag tggtatcctt
     1021 tatttttcgg tcaaaccggt atcctgtctg ggaagaaaac cgattgaaga agcgggcagt
     1081 cttgttctta ccggtggatt ttccctgcca gcggaagctt tgattgaatg aagtattgag
     1141 cgtggggata aattgattgg taagaatgtt gacacggaca taattccgca tctccggaaa
     1201 gccctcagta gtaaattcat tgacttgttg aatagaatca ttattataat cattccacac
     1261 atactggcct gttccgggtt gtacggcgag ataattgaac tctgtttttt gttcctgacc
     1321 tgagctgact tcaaagagtg tattgccgga tagcctgttt tttaaggcgg aaaacagata
     1381 atcaagttgc cccagatagg tatccttgga gttgaatttg gctcctggtt tgatgcgatc
     1441 agcatcgaac gttctcactg tggccgtaag gttgagcacg gaagaaagac cggacttcag
     1501 ggttgatttg atgccgtatt ccagggcatg atttgccagg agcaagtcat gagtaaatgg
     1561 aagatagtca ttgcgccgct gtatataaat ctcagaactg aaattcttgg aaaaagtatt
     1621 ttgagcagat aatctcatta catgaaaggc aaaggaggtg gcaagtaagg tgtcggaaga
     1681 gacggagcgc cgcctgttgt attcgccctg ataagtagca atcaggttta gtccgcccaa
     1741 tcgggggaag gctctcctga tgcttacatt tggtctgagg aatgctgagg tttcattgat
     1801 actttccgac ttgaggtaac tggcatcagc acggaactca aactttttta atttgtaatt
     1861 gagggcagcc tcttgtttta ctcccttgaa atgtatcggc tgaaacaaac cggacagtgc
     1921 ataattgagt cttaactggg tgccgatgtt ctgattcagg aaaagtcgta aatactgttg
     1981 ggattcaagg ggaatactgg cgatgttcca gtcacgattg aattcctggc ttcgatatgg
     2041 tgtgattgga ttgaattgaa gccctgtatg ctcatattct cctccgacac tcgctgtcca
     2101 tttcctttta tctgatacca gtatgttttt tctttcggca tatacatgaa cgccaagccc
     2161 atagttatct gcatcatctt cgcttgaaaa aagattgttg tcctgccctg acaatgctgt
     2221 ttccaatcga attaccgtat tggtgtccgg cttccacgct gcacccgcag taaacatttg
     2281 ccgctgtgct ggagcagtaa gcctggaaac aggattatat ctcccctttg gcctcccgtc
     2341 tgcatctatg cctgcaaatc tatatacacg gccattgatt ccatcattca cacgatcgta
     2401 ataaccattg ttctgaccga catccgtaaa gtcggccaga tacctcttac taccctcctt
     2461 ccatggaaaa actaaaaagg aaaacggctg attgttgatg atcgtatcat tcagctgata
     2521 tgaaacaacc acacttgtat tggaagggtc tatctcccgg attgaattga ccaatttatt
     2581 cgatatgctg tccccggctt gctggagcgt cattctgtcc tctgcactca attcacggac
     2641 acggtccgaa tttttgctgt cgcgctcgtt ataggcattc aggttaaaat cccatttttt
     2701 ggtctgatag cggacattca ggttggcaaa ggatctcaga tattgctgat tggagtattc
     2761 gtattccacc acaatcctcg aatccttggt gatgatgcgc gttggcgtaa aggttatctc
     2821 accgctgtta taatcaataa tgtaatcccg gtcaaaaccg cgcaccaact ggattccgtc
     2881 cagatacaca cgctctgtac ccgctaaaac aataacaaaa gcctctcctt ccgcgccggg
     2941 aagacgatac gggccctgat taccttccgt aacagttagg gtaaatcgat tgaactttcc
     3001 tttggtaacg gcaataccac cacttgtact caacttgttg cccttgagag tggccgtggt
     3061 ggaagcttgc actccctgga gttttttgaa ataattgagg aagtagccgg gggggcgatt
     3121 gagctcgaag tcacctactg tgagactgct gtttttcctg gaaagcacaa tgtaaatctt
     3181 gtcgaattcc tgcagttgtt gtgaattacc ttccggctga atcggaatag agttgtcgga
     3241 cagagcacct acaatcttaa tatcatcacc caactcaccg gagagttgca tgttgaatgc
     3301 actgttgagt gctaaacttt gggtgttacc aagtgaaatg cctcgggtaa atgctccgcg
     3361 ataattcatg gtgttgcgtt caggaataag aggcgaagag gcataccgca gttctctttc
     3421 agagagggtt gggtcccaga cctgcaatat catcgaagtg tccatcaacc tgtatggaag
     3481 agaaaggttc ttagaaaata tgcgatagct caataccacg gtttccatgg aaaggctgtc
     3541 cggcaaggga attctgcgtc tgacaatatt gttgctgatt tcgaagaaat cgttcaacac
     3601 aagagtcgac ctgtcttgca ggtaaaacga ccatgttccc ggaaccggcg tttctggtat
     3661 cgataaaggc aaactatcag gatgaaaaaa tatggaccga aggttgccca gtgggcccat
     3721 cacttgcgcc tttgccgata tcgacaataa caaggcagga atgatatagg acacatattt
     3781 tctaaaccgt atcaacaagc aaatccggaa taattttaca atcagagtat aaaattaacg
     3841 aataatatca caaagatggt attgcctgat aacaggtgca aggacaaagg gaatatgcat
     3901 ggaattaaag aattgaaaac aatgatgcca tgaaacacaa atccatcctc ctgcatgaaa
     3961 tatcgatacc cggactaaca ctttgtaata agtccggagc atcaaacaag cgttctaagc
     4021 aaggacggtt attgcttttt cacccttagc tgttattctt ccaataggtt ctgtaaaggc
     4081 atcgtaacca ttgtctgtta atatttctct ggcagcctcc aggccgtcgg gagatactgc
     4141 aatgagtaag cctccgttgg tctgtgggtc tggcaggatg cgaaatgcgt ccattatatt
     4201 gatttcaggg ctcaactcga tcttagcgcc ataaccattc cagttacgat aggtcgcatc
     4261 cggcataata ttctgtgcaa gatattgatg cacaccggca agaataggca ctttcgaata
     4321 ctcaagaacc gctgataagc ctgagccttc caccatctct accaggtggc cgcccagtcc
     4381 aaatccggtc acatcagtca tagcatggac tgaatccaac ttacccaatt gtccaccgac
     4441 actgtttaat cgagtcagct ggctgaataa aatctccaga tcttcttcgg tgattgcccc
     4501 tcgcttgccg gctgtggaaa gaattcccac tccaagtggc ttggtcagaa gcagcaggtc
     4561 gccttcccgg gctgtatcgt tgcgcttgag atgacgcatc gataccctgc cactaacggc
     4621 aagcccaaat atcggctcag gagtatctat gctgtgtccg ccggccaacg gtattccggc
     4681 ttcattgcag atcgtgatag cgccatcgag gactgcacgg gcagcttcca ctggcagctt
     4741 atcaatcggc cagcccagga ttgccagtgc catgatgggg tctcccccca ttgcataaac
     4801 atcactgatg gcattggcag ccgcaacttt cccgaagata aaagggtcat caacaattgg
     4861 ggtaaagaag tcggttgttg aaataagtgc ttcgttgtcg ttgagcctat atactgccgc
     4921 atcgtccctg gagccattgc caacgataag cttacctgaa gtgatgccac tgacactgcc
     4981 cttcagtatt tcatccagca cttgtggagc gatcttacaa ccacagcctc caccatggga
     5041 atatttggtc aaacgaatct tttcttcagt cataaatcct ttgttttttg gaggatgagt
     5101 ctggcatttt cataagcatt ggtaccgggc aattcgatgg tcatgatagc cggttgataa
     5161 attccaccgg aagcatagaa tttatcataa tacttcagca ggatgtcaaa ggcatcacgg
     5221 atacaaccgg catcaagatg agccaatgca ttctttgttt caagcccgcc cagccgcttt
     5281 tgaatcctca aaacagcatc cttcagtttt tctgtactaa attggccata gcctgcagta
     5341 atataaccca ggcgtgcctc atgggacacc tcggtgtgaa tccttggtgc cgccttcatt
     5401 tgattgaaaa aagtatgaaa gatattaaca cagccaatcc tgttgctctc actttcaagc
     5461 caaatcgtct tgccggggtg ttccctacta atcgtataca actcctgtgc aagcttgttt
     5521 tcaaattgct ccaccgttgg ttgaggcggc atgcccaaag cgccaaatgc cgatcctcga
     5581 tgccctgcaa gaccttcaag atccaccgtc ggtacaccca gccgagccat ttccagaaga
     5641 atttccgtct tgccgcttcc ggtaaaacca ctcaatatcc taagtgaata aggcctggaa
     5701 aactggtcca atacccaatt cctgtatttt ttgtagcccc ccctgagcga aaagactcta
     5761 aatccataca tgtcgaggag ccagctcacc gcaccactgc gcatgcctcc gcgccaacag
     5821 tgcaaaagaa ctgtattggt acccttatga tgcaggatat cctccaccag gcagaccatc
     5881 tgcctcatct tgataccaaa ataatccagg ccgatcttga tggctgcttc ccgacttctt
     5941 tgcttataag tcgttcccac ctccctcctt tcatcatctg aaaagagcgg cagtggcagt
     6001 gctccgggta tatgcgcatg tccatactcc gaaggactcc gcacatcgaa aaccggatgt
     6061 tcttcggcca gttgcaggaa ttgctcaata tctatttgct gaactgccat aatgcaaagc
     6121 tattaatttt caaaaacaat caggcaaaca atggtgtagg atcacgccaa taccatctat
     6181 agaaaaacag tttctcctgc aatggccggg atgttccatt gcatgtgttg atacaccaac
     6241 ctgagaggtc tgccaaggtc aaaaatgtgg cagatgacaa gtgctaccga agtaggtaga
     6301 tgattaatag attgcgaaat tgatttccct atatgatcgg gcgattggta tgaaaatcct
     6361 tgcccatttg tgaagtaaa
//
LOCUS       DAOQQN010000175         6363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5227_length_6363_cov_5.797876, whole genome shotgun sequence.
ACCESSION   DAOQQN010000175 DAOQQN010000000
VERSION     DAOQQN010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5227_length_6363_cov_5.797876"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..3097
                     /locus_tag="PLG24_07560"
     CDS             326..3097
                     /locus_tag="PLG24_07560"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMZ24783.1"
                     /translation="MNLLFRINRLKTAFFVGLALMILPSLIQAQVEGFVKGPSVEGIT
                     EYTLTKNGLKVLLFPEPSKPTITVNITYLVGSRHEGYGETGMAHLLEHMVFKGTPNHP
                     DIPSELTKHGARPNGTTWLDRTNYFETFAATDENLEWAIDLEADRMVNSFIAKKDLES
                     EMTVVRNEFESGENNPISILMERVISTAYLWHNYGKSTIGARADIENVPIERLQAFYK
                     KYYQPDNAVLVVAGKIDVNKTLELIKKHFGKISNPDRTLAPLYPTYTLDPVQDGERSV
                     VLRRVGDVQVVMAAYHVSPGAHPDFVALDLLASILTDNPSGILYKELVENNKATSVDG
                     FGFELKEPGIMMFGATVPLDKSLEEAKMILLGRLDKVKGMTFTQEELDRQKQKMLKSW
                     DMTFNNVERIGTGLSEYIAQGDWRLMFINRDNIKKVTLEDLQAVATKYFKNSNRTIGE
                     FIPDKNPDRVEVPGPIDVAAVVKDYKGNAAVASGEAFDPSPNNIDERTTVAKSKSGNF
                     EVAMLPKQTRGNVVIANLTIRFGELNSLKNKSSIGDLTAAMLDKGTKTRTKQQITDEF
                     DKLKSRVSFSGGATSCNVRIETTKENLKPTLAIVADILKNPSFPQNEFDIMVTQEVSG
                     LEEQMSDPQGLAGIEFARAMSSYPKDDPRYTPTLSENIEMLKAVKLGDLKKFHSDFYG
                     ASKATASVVGDFSKAEIEKMLLETFGNWKSKMPYKRIASPYQPTKAGEKTINTPDKSN
                     ALYLAGYSIEISDTDPDYPAMVLANYILGGGFLNSRLATRIRQKEGLSYGVGSGFSAN
                     ALDKTGRFMVYAISAPENSERVNICVKEEITKANKEGFTAEEIEAAKSGLLQSRQVSR
                     AQDASLAGTLNSNLFLGRTMQFDGKIDDAIKALTPDQVNKAFNKFISADKLVVVRAGD
                     FEKDVEKKP"
     gene            complement(3306..4943)
                     /locus_tag="PLG24_07565"
     CDS             complement(3306..4943)
                     /locus_tag="PLG24_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HMZ24784.1"
                     /translation="MKKCFFLFLFIGLGTWSVAQRNIITDQIIVKIKDCYLQNSRDKS
                     NLAPSITQSFTLLGERFKALDTKNLYLGKNHSMAYFTILFPPGSDIDEIITEYKKLEE
                     IEYAEPDYIGYGAGGDGFIPDDKFYVKQWGLNNNGTFQHAQGIAGIDVNMEESWSITT
                     GDSNIIVAILDSGGKLDHPEFAGRIWQNHKEISGNNVDDDENGFTDDFQGWDFVNKDN
                     NPSDDHGHGTNVGGIVGSTGNNGRGFAGVDWNCKLMFLKVLNSNNTGYQSSFIAAIYY
                     AVDHGARVINMSIGGGQSDIYRDAVNYALQKNVTIVACMMNHNTEAPYYPAAFPGVIA
                     VGSIDPNGMRTQPFFWNSSSGSNFGSHISVVAPGNYIYGLSHSSNSNYDTYWGGTSQA
                     TPHVAGVVSLMLAINPNLTPKEIKNILETTARDGIGRPEEDTPGWDKYHGWGLIDSYK
                     ALTYSVSSRKNPVHFPDFSVFPNPARSALQLIFQTPVTEGEVYILDLLGRQVYSNQKI
                     NGDKLEIDCSEIPTGIYTIVLQNKGSTHTGGKLLIIH"
     gene            complement(5140..5598)
                     /locus_tag="PLG24_07570"
     CDS             complement(5140..5598)
                     /locus_tag="PLG24_07570"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HMZ24785.1"
                     /translation="MKKLILPSLIIALFLVACNFNNKTPSNDTKSVKLPPDPSSIVYN
                     TDWILKDMNGKEVKISPDAPKKAMIHFDKEDHVTGSGGCNLFSGNCKFIDNEKIAISY
                     GAATQMACPEMDIESEFFATLGKAEKYKLDGNKFYLMDKNGTVLLSFTKD"
     gene            complement(5738..>6363)
                     /locus_tag="PLG24_07575"
     CDS             complement(5738..>6363)
                     /locus_tag="PLG24_07575"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMZ24786.1"
                     /translation="THGFQMCTHAIGDSANRVLLETYAKYLKGPNDLRWRIEHAQVID
                     ANDFKFFSDYNIVPSVQPTHATSDMYWAGERLGKERLINAYAYHHLMEQNGWIPLGTD
                     FPVEDISPFKTFYAAVVRKDAKGYPEGGFQPENALTRKEALRGMTIWAAKSNFEENEK
                     GSLEIGKWADFIITDRDLMTTEESDILGTKVLKTFIAGEKVFEADKK"
BASE COUNT         1846 a         1282 c         1323 g         1912 t
ORIGIN      
        1 cctgattcta tgggactttc gggagagctg ggtaaaatat cgtttcttta tggagcagat
       61 aaatggaaaa gcacaccagg attgcccatg gttacagacg attgggttat taatcatcta
      121 cctggttcat atttttaccc caacaccctg taatatttat tctcatccat cgaactgaat
      181 gtgcgactag tttttctcct actacgtaaa attaatctag tgtcatttgg tcgaacgatt
      241 cccttcattt gacgattaaa aaataggaaa gtaagctttt ttgtaaatta gcgtcatttt
      301 ataacctaac ttaaccttaa ttcaaatgaa tttacttttt aggataaacc gattgaaaac
      361 ggcgtttttc gtcggtttgg ctttgatgat tttgccttct ttgatccagg ctcaggtaga
      421 gggcttcgtt aaaggacctt cagttgaagg aatcactgaa tacacactca ccaaaaatgg
      481 actaaaagtg ttgctttttc cggaaccttc aaaaccgacc atcacagtta atattacgta
      541 cttagttggt agccgccatg aaggatatgg tgagaccggg atggcacact tgttggaaca
      601 tatggtgttt aaagggacac caaatcaccc tgatatcccg tccgaactga caaaacacgg
      661 tgcaagacca aatggaacaa cttggttgga tcgaaccaat tatttcgaaa cctttgcagc
      721 tacagatgaa aacctggaat gggcaattga tttggaggct gaccgtatgg tgaattcgtt
      781 cattgcaaag aaagacctcg aaagtgagat gacagtggtt agaaacgaat ttgaaagcgg
      841 agaaaataat ccgataagta ttttgatgga aagagtcatc tcaacggcat atttatggca
      901 taactacggg aagtcaacca ttggtgctag agcggacatt gaaaatgttc caattgagcg
      961 gctacaagcc ttttacaaga agtactatca acctgataat gcagttttgg ttgtagccgg
     1021 taaaattgat gtaaataaaa cacttgaact gattaagaag catttcggaa agataagtaa
     1081 tccagacaga accctggcgc ctctttatcc aacctataca cttgatcctg ttcaggatgg
     1141 ggaacgtagt gtcgtattac gacgtgttgg tgatgttcag gtagttatgg cagcttatca
     1201 tgtaagcccg ggtgctcatc ctgactttgt agcattagac ctcctcgctt caatccttac
     1261 cgataatcca agtggcattc tttataagga actggtagag aataacaaag cgacctcagt
     1321 ggatggattt ggatttgaat taaaagaacc cggcattatg atgtttgggg caaccgtacc
     1381 tttggacaag tcccttgaag aagcgaagat gattttactt ggccgtcttg ataaggttaa
     1441 gggtatgacc tttacacagg aagaactcga ccgccaaaag cagaagatgt tgaaatcctg
     1501 ggatatgacc ttcaacaatg tagaaagaat aggtacaggt ttgagtgaat atatcgccca
     1561 gggagactgg aggcttatgt ttatcaatcg tgataatatt aagaaagtta ccttggaaga
     1621 tcttcaggcc gtagctacga aatattttaa aaacagtaac cgtactattg gagaattcat
     1681 cccggataag aatccggatc gagtggaggt tcccgggcca attgatgttg ccgcagttgt
     1741 aaaagattac aaaggaaatg ctgcagttgc ctcaggtgag gctttcgatc cttcaccgaa
     1801 caatatagat gaaagaacaa cagttgctaa atccaagagt ggtaattttg aggtcgctat
     1861 gttgcccaag cagacaagag gcaatgtcgt tattgccaat ttgacaataa gatttgggga
     1921 gttgaattcc ttgaaaaata aatcctctat tggagacctt accgcagcaa tgcttgacaa
     1981 ggggacaaag acacgaacca agcaacagat tacggatgaa tttgacaagc ttaaatcaag
     2041 agtttccttc agtggcggtg ccacttcatg taatgttcgg atagagacga ccaaggaaaa
     2101 tttaaagcca acacttgcca ttgtcgcgga tattttgaaa aatccgtcat tcccacaaaa
     2161 cgaatttgat attatggtta cgcaagaagt tagcggattg gaagaacaaa tgagtgatcc
     2221 tcagggatta gcgggaattg agtttgcaag agctatgtcc agctatccaa aagacgatcc
     2281 ccgttataca ccaacattat ccgaaaatat tgaaatgctt aaggcagtca agttgggtga
     2341 tctaaagaag tttcatagtg atttctatgg agcatccaaa gctactgctt cagtagtagg
     2401 tgatttttca aaggcagaaa ttgagaaaat gctcttggag acatttggca actggaaatc
     2461 aaagatgcct tacaagcgca tagcaagccc ttatcaacct actaaggcgg gtgaaaaaac
     2521 aattaataca cctgacaaat ccaatgctct ttatttagcc ggatattcta ttgaaatatc
     2581 agatactgat ccggactatc cagcaatggt tttggcaaac tatattttgg gaggaggctt
     2641 tctcaactca agacttgcca cgcgtatccg ccagaaagaa gggctgagct atggtgtcgg
     2701 atctggattc tcggctaacg cattggataa aacagggcgt tttatggtat atgcaatctc
     2761 tgctcctgaa aactcagaaa gagtaaacat ttgtgttaag gaggaaatta ctaaagcgaa
     2821 taaagaaggc tttaccgctg aagaaatcga agctgccaaa tctggcctat tgcaatctcg
     2881 ccaggtaagc cgggcacagg acgcatccct ggcaggaaca ctcaatagta acctttttct
     2941 tggtagaacc atgcaatttg atggtaagat cgacgatgcc attaaggcac tgacaccgga
     3001 tcaggtcaat aaagcgttca ataagtttat ttcagcggat aaattggtag ttgtccgggc
     3061 aggtgacttt gagaaggatg ttgaaaagaa accttgatag atatattttc atttgtcatt
     3121 gtgaaaaggc cggaattgct ccggcctttt ttatattcca agatgacgtt atttattaat
     3181 ccatctatgt ctgctggcct gctttattct tccatactat cttcaatcct ttacccatag
     3241 tgttttgtgt catccggtga tcacattttg aaggaaaatg gaagaacaaa attggttaaa
     3301 tgaatttagt ggatgatcaa taacttgcct ccggtatgag tactgccttt attctgaaga
     3361 acgatagtgt agataccggt tgggatttca ctgcaatcaa tttctaactt atctccattt
     3421 attttttgat ttgaatagac ttgtctgcca agaaggtcca gaatatatac ctctccttct
     3481 gttacgggtg tttgaaagat aagctgtaat gcactgcgag ccggattagg gaaaacagaa
     3541 aaatccggaa aatgtactgg atttttccgg cttgatactg aatatgtcaa ggccttgtat
     3601 gaatcgataa gtccccatcc gtgatatttg tcccatccag gagtgtcctc ttcgggcctt
     3661 ccaattccat cacgggccgt cgtttccaga atatttttga tttcctttgg cgtcagattg
     3721 ggatttattg ccaacatcag cgatacaaca cctgcaacat gaggagttgc ttgagatgtt
     3781 ccgccccaat aagtatcata attcgaattg gaagaatggc tcaatccata aatgtaatta
     3841 cccggtgcca caactgaaat atgacttcca aaattgcttc cactgctgct gttccaaaaa
     3901 aatggctgcg tcctcatacc gttcggatcg atagagccta cagctattac tccgggaaaa
     3961 gcagccggat aataaggggc ctctgtattg tgattcatca tgcaagccac aatagttacg
     4021 tttttttgca aagcataatt tactgcatcg cggtatatat cggattggcc cccacctata
     4081 gacatgttaa tcaccctggc accatgatcc accgcataat atatggcagc gatgaagctt
     4141 gattgatacc cggtattgtt tgaattcaat actttcagga acataagttt gcagttccag
     4201 tcaacaccgg caaagcccct gccattatta cccgtagagc caactatgcc tccgacattg
     4261 gttccatggc catgatcatc ggaaggatta ttatctttgt ttacaaagtc ccatccctga
     4321 aaatcatctg taaatccatt ctcgtcatca tccacattat taccggagat ttccttgtga
     4381 ttctgccaaa tgcggccggc aaactccgga tgatccaact tcccgccact atccaatatg
     4441 gccacgataa tgttgctgtc gcctgttgtt atggaccatg attcttccat gttgacatca
     4501 attcccgcta ttccctgtgc gtgttggaaa gtaccgttgt tgttcaagcc ccattgtttt
     4561 acataaaact tatcatccgg tataaatcca tcgccaccgg caccatagcc tatataatca
     4621 ggttcggcat attcaatctc ttcgagcttt ttgtattcag ttataatttc gtcgatatct
     4681 gatcctggag ggaaaagaat ggtgaagtaa gccatgctgt ggttcttacc caaataaaga
     4741 ttctttgtgt ccagagcttt gaatctttct cctaatagag taaaggattg ggttatcgat
     4801 ggagctaaat ttgatttgtc acgggaattt tgaaggtagc aatccttaat tttgacaatg
     4861 atctggtccg taataatatt tctttgcgct actgaccatg taccaaggcc aataaaaaga
     4921 aataaaaaga agcatttttt catggcaaga attttgtcgg tattgtattt tttattctct
     4981 cccttcaaac tctttgtaat cttaaaaatt tacttgattc agaacatcaa tggcctatgg
     5041 tttgctttaa gcagaggttg attatggtca aaccgaaatt ctctttcatt cgaaagaaat
     5101 gttgataatt agaaaatgaa agcttaaact gtccggtcat caatcttttg tgaaggaaag
     5161 cagtacggta ccgtttttat ccatcaggta aaatttattt ccgtcaagtt tgtatttttc
     5221 agctttgcca agtgtggcaa aaaattcact ttctatatcc atttccggac atgccatctg
     5281 tgtggcagct ccataagaaa tcgcaatctt ttcattgtcg ataaatttgc aattacctga
     5341 aaataagttg cagcccccac tacctgtaac atgatcttcc ttgtcaaaat gtatcattgc
     5401 cttctttggc gcatcaggac tgattttaac ttccttgccg ttcatgtcct tgagtatcca
     5461 gtctgtatta tacacaatgg atgaaggatc cggtggtaat tttacacttt tagtatcatt
     5521 ggatggagtt ttgttgttga agttacaagc tactaggaat aaggcgatta tcaaggatgg
     5581 aagaatcagt tttttcattt tattcaatat ttaattttta tttcattttg tcaggaattt
     5641 actttccaac aatatcgatg ccggaaagat tcaattgtca cattctaatt gacagaattt
     5701 cataacctga ctatcttttc atctacggga atactgttta ttttttatct gcctcaaaga
     5761 ccttttctcc ggcaatgaaa gttttcagaa ccttggtccc taatatatca ctttcttccg
     5821 ttgtcatcaa atctcggtcg gtaatgataa aatctgccca tttccctatc tcaaggcttc
     5881 ccttctcatt ttcttcaaag ttgcttttag cagcccagat ggtcatacca cggagcgctt
     5941 cttttcgcgt cagggcgttc tcgggctgga atccaccctc agggtagcct tttgcatctt
     6001 tgcgcaccac ggcggcgtag aatgtcttga atggcgaaat gtcctctacc ggaaagtccg
     6061 tccctagtgg aatccagcca ttctgttcca tcagatgatg ataggcatag gcatttatca
     6121 atcgttcttt gccaagcctt tctccggccc aatacatatc tgaagttgca tgggtaggct
     6181 ggaccgatgg cactatgttg taatcactaa aaaatttgaa atcatttgca tctatgactt
     6241 gtgcatgttc tattctccag cgaaggtcat ttgggccttt aaggtatttg gcgtaggtct
     6301 ccagcaatac tctgttggcc gagtctccga tggcgtgagt gcacatctgg aagccatggg
     6361 tat
//
LOCUS       DAOQQN010000176         6346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5250_length_6346_cov_4.524559, whole genome shotgun sequence.
ACCESSION   DAOQQN010000176 DAOQQN010000000
VERSION     DAOQQN010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6346
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5250_length_6346_cov_4.524559"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="PLG24_07580"
     CDS             complement(<1..1273)
                     /locus_tag="PLG24_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HMZ24787.1"
                     /translation="MKRYFFTASLFIFLLTNLHAQNITGDWNGTLNAMGFKLRLVFHI
                     TSQNDSLVAMMDSPDQGASGIPTSLVTFRHPDLHIEVGKGMIVYKGSMVTPDSIEGTF
                     SQSGRTFDLALKRGNVETRRPQEVKPPFPYKSEDITFANPTAGITLAGTLTMPEKGMK
                     FPAVVMISGSGPQDRNETIMGHKPFLVIADYMTKRGIAVLRFDDRGTASSQGKFDGAT
                     SADFATDVEAAVAYLRERKEIDSDKIGLIGHSEGAIIAPMVAAKDKGIAFIVMLAGTG
                     FDGADILVMQNEALGRASGLDEKDIEKATKINRILYNELVTGPGDSTLVEKTFVSLKP
                     LLSEKMSDEEILSFTDKSIKQLNTPWILYFLKNNPATFLEKVKCPVLAVGGSKDLQVP
                     ARTNLDAIEKALHKGGNKRVTIREFTGLNHLF"
     gene            complement(1270..1848)
                     /locus_tag="PLG24_07585"
     CDS             complement(1270..1848)
                     /locus_tag="PLG24_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24788.1"
                     /translation="MKQILFNPFRTIAGAKALLIGWIIMLITACIAMYSQCHFDGAID
                     VHIGRNTTAIAYFLEPLVNWSVLTVIFYFTGLLLSTSHIRWIDFAGTVALARYPYLLA
                     AFIAFAMPTLDPESLTNFRDIDTSTLASLMITSILILLVSIWMIGLLYRAFTISGNLR
                     ERQAVWGFIIALILSELITGIIFHYLYKNLFL"
     gene            complement(1848..2120)
                     /locus_tag="PLG24_07590"
     CDS             complement(1848..2120)
                     /locus_tag="PLG24_07590"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HMZ24789.1"
                     /translation="MLNKRMPHSCPACTAALEVTQLGCPACGTVISGNFPLPELLTLN
                     SDDQQFVLDFVRSSGSLKLMAQNLKLSYPTVRNMLDDIIEKLNQYP"
     regulatory      3470..3514
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3512..4147
                     /locus_tag="PLG24_07595"
     CDS             3512..4147
                     /locus_tag="PLG24_07595"
                     /inference="COORDINATES: protein motif:HMM:NF008406.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HMZ24790.1"
                     /translation="MHIKKQTTKGLALILALIHVLIIAASNYLVQFPITVVGFHATWG
                     AFTFPFIFLTTDLTVRLFGAPMARRIIFYAMLPALLVSYVVTVLFRSGTWLGLETLMT
                     FNLFVFRIALASFSAYVVGQVMDIFVFNWLRQHQQWWHAPLASAIIGNLVDTYVFFIL
                     AFHNTSDVYLAENLWEIAAVDYTFKILVNAVIFLPMYKVILDKVSRRILVG"
     gene            4932..>6346
                     /locus_tag="PLG24_07600"
     CDS             4932..>6346
                     /locus_tag="PLG24_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24791.1"
                     /translation="MKDVMSFLLFLTFSMLGNIGMAQHGKVLMLDGDNDYMSVADHED
                     LDINSGESFSITCWVKTFESADFYRIASKRSNAAGGSVGYEMITNKSGGQFGINLRSA
                     AGVNAGPPFGEIPVTDGKWHHLALIFDAQSKASRIYVDGNLDNFLNSNVIGVQEFSND
                     VALLFGTSIGLTGFWNGWMDEIRIWSKALTISEIKDDMPAILNGPVDNLIAAWDFETV
                     QNGIVPDITGNHPGQLIGDAKTVDDNSGSATITAVTRYQSAIPIGQGNKDERLASANF
                     KVSGEGNNLVVEYLRFKVESDANTIGRCKLYYNGINERLNLVTAELLGEADVSGGYVE
                     FSFNKTLVEGNNVFWLCGDIPGSASEGAQIGSWLSFHTVSGIEAVMDDPATPVFRTVL
                     LQHQLLFSGGDFGSATYRIPAIASRGDRIVVVADARINNNGDLPNNIDLFARYSEDGG
                     KTWSGPVTVADFGDSGASDPA"
BASE COUNT         1686 a         1202 c         1419 g         2039 t
ORIGIN      
        1 ggaacaaatg gtttaatccc gtaaattcgc ggatggtcac acgcttattc cctcccttgt
       61 gcaaggcttt ttctatagca tccagatttg ttcgggcagg cacctgcaag tctttcgatc
      121 caccgactgc taataccggg catttaactt tttccaaaaa agtggcaggg ttgtttttca
      181 gaaagtatag aatccaaggt gtgttcagtt gtttgattga tttgtctgta aatgagagga
      241 tttcttcatc gctcattttt tcacttagaa ggggcttaag agagacaaaa gttttttcta
      301 caagtgtgga atcacctggt ccggtgacga gttcattgta cagaatgcga ttaatcttgg
      361 tggctttttc tatatccttt tcatccagtc ctgatgccct gcctaatgct tcattttgca
      421 tcactaagat atcagcccca tcaaaaccag tgccggcgag cattactata aatgcaatcc
      481 ctttatcctt tgctgccacc atgggggcga tgatggcacc ttcactgtga ccgattaaac
      541 ctattttgtc tgagtcaatt tcttttcttt cgcgcaggta tgccactgcg gcctctacat
      601 cagtggcaaa atccgctgat gtagctccgt cgaatttacc ctgcgaggat gcagtgcccc
      661 tatcgtcgaa ccgtaatacc gcgatgcctc gctttgtcat ataatcggca atgactaaaa
      721 acggtttgtg tcccatgatg gtttcgttcc tgtcttgggg cccactgcct gaaatcataa
      781 ctacagccgg aaatttcatt cctttttccg gcatggtcag ggtgccagcc aatgtgatac
      841 ctgctgtggg attggcgaag gtaatgtctt cgcttttata gggaaatggc ggtttcacct
      901 cctggggtct tcgagtctca acattaccgc gctttaaagc cagatcgaag gtcctgccgc
      961 tttgtgagaa tgtcccctca atgctatcgg gtgtgaccat acttccttta tatacaatca
     1021 tacccttgcc tacttcgata tgcaggtcag gatgtcggaa ggtgaccagt gaagtaggaa
     1081 tgcctgaagc tccctggtcc ggactgtcca tcattgcgac caggctatcg ttttgtgaag
     1141 tgatgtggaa taccagtcga agtttaaacc ccatagcgtt gagggttccg ttccaatccc
     1201 ctgtgatgtt ctgtgcatgg agattagtca aaaggaagat gaataaagat gcagtgaaaa
     1261 aatatctttt cataggaata aatttttata caaatagtgg aaaatgattc cggtgataag
     1321 ttcagataaa atcagagcaa taataaagcc ccagacagct tgcctttcgc gcagatttcc
     1381 agagatagtg aatgcgcgat acaaaagtcc tatcatccag atcgaaacca acaatattaa
     1441 gattgatgtg atcatcagac ttgccaaagt ggaagtgtcg atatcccgga aatttgtcag
     1501 gctttccggg tcgagtgtcg gcatagcaaa agcaatgaat gccgccaata aatacggata
     1561 tcttgccaaa gccactgtac ctgcaaaatc gatccatcga atgtgggaag tagaaagcaa
     1621 cagaccggtg aagtagaaaa ttacggtcaa tactgaccaa ttcaccaatg gttccagaaa
     1681 atatgctatt gcagtcgtgt tccggcctat atgtacatcg atggctccgt caaaatggca
     1741 ttgactatac atggcaatgc atgcggtaat cagcatgata atccatccta tcaaaagggc
     1801 ttttgcgccg gcaatcgtcc ggaaaggatt gaacaagatc tgcttcatta tggatactga
     1861 tttaattttt cgatgatatc gtccagcata ttgcgaacag tcggatagct caattttaag
     1921 ttttgcgcca tgagtttgag gctaccgctt gagcggacaa aatcgaggac aaactgttga
     1981 tcgtcgctat tcaaagtgag taattccggg agtggaaagt tacctgagat taccgtacca
     2041 caggccggac agcccaattg tgttacttcg agtgccgcag tacatgcagg acaggaatga
     2101 ggcattcgtt tgtttaacat tatttatttt tatattcaca aaaataaata attatttaat
     2161 atagttaaac tataaataat aattagttaa ttcaacataa gatactatta taaatgaatc
     2221 tgctaaatta ttatgagggc ttccgaatag atacaggagg agacggattg atcattcttc
     2281 gacttcaatc atgtgtttga gcctaaatag ttgccgaaca tcttggagtt gatgggtgtg
     2341 acaatacttc atgagattgg gtttacggtt attatcatct ccggcagtgt catgatatgg
     2401 cggaatgatc attaagatgg tgggatgatg gcatataaag aatttcagta ggtttttgaa
     2461 atagatatgg gagttgccgc ctccgaagta ggtagatgat taatagtcac aattattgca
     2521 ggtattgcct tatgggttga cctttccaaa attcaggtaa tttattcggg aattaaattt
     2581 ttcatttgtc cccaaatctt tatgtatggc agtttttgaa ttattacttt cctacctact
     2641 tcgatttttg gggaatgggg gtggtgccgc ctctgaagta ggtagatgat taatagtcac
     2701 aattattgca ggtattgcct tatgggttgg ccttgccaaa attcaggtaa ttattcggga
     2761 attaaatttt tcatttgtct ccaaaacctt gtgtatggcg gtttttgaat tattactttc
     2821 ctacctactt cgatttttgg gagaatgggg gtgatgcagc ctccgaagta ggtagatgat
     2881 taataaccac catgcttgac agggcggcct ttcggggtgg ctttgccaaa attcagatga
     2941 tttactcggg aattaaattt ttcatttgtc cccaaatcct tgtgtatggc agtttttgaa
     3001 ttattacttt cctacctgct tcaatttttg gagggacagg gttatgccgc cttcgaagta
     3061 ggtagatgat taatagtcac aattcttgcc ggtactgctt ttcgggatgg ccttgccaaa
     3121 attcaggtaa ttattcggta gttaaatttt tcatttgttc ccaaatcctt gtgtatggcg
     3181 gtttttgaat tattactttc ctacctactt cgatttttgg aagaaaaaaa ttgaaatcaa
     3241 caccataata cacagtcttc ttatctcgtc gacctacaga aaagcctttc atgcattttg
     3301 tcagtttaat tgcgttacaa tctgaaagct gccctgctct tgagccttgg tttgtaattt
     3361 ttggagtaga tcactttgaa cttttactat ttggtgataa ttatcccgga acacctatct
     3421 ttgttgcgat atttaattta ttgatagcag aaatctttca ggacattgga aagacgtggt
     3481 tcgaaatcct cccacgataa aaaactaagg tgtgcatatc aaaaagcaga cgactaaagg
     3541 ccttgcattg atccttgcgt tgatacacgt cttgatcata gctgcaagta attacctggt
     3601 acaatttccg attacggttg ttggcttcca tgcaacatgg ggtgcattca cttttccttt
     3661 cattttcctg acgaccgatc tgacggttcg tctttttgga gcccccatgg cgaggaggat
     3721 catattctat gctatgttac cggcattgct ggtatcttat gtcgttacgg tattgttccg
     3781 atcgggcaca tggcttggcc tcgaaactct gatgaccttc aatctcttcg tattcaggat
     3841 cgccttagcc agtttttctg cttatgtcgt aggacaagtc atggacattt tcgtattcaa
     3901 ttggctgagg caacatcaac agtggtggca tgcgccactg gcttcagcga taataggcaa
     3961 tcttgtcgat acttatgtgt tttttatact tgctttccat aatacttctg atgtctatct
     4021 ggccgaaaac ctttgggaaa tagcggcggt ggattataca ttcaaaattc tggtcaatgc
     4081 tgtgattttt ctgccaatgt ataaggtgat attggataag gtaagccgta ggatactggt
     4141 gggttgatgt gcaagtgtct ttcagattac aagacaacta tgcatcaaag ttcaggattt
     4201 ttttggacac ttttttcctt ttattacaaa ttcgtttgga atcgaaccat gaaaggctga
     4261 tgctttcttt catggatgtt atctgatctt ttttcagaga tagaccaatc gccggagact
     4321 ttaagaactg gtcaggcctt tcaaaatagg tatttaccta gatacttctt ctatcaacta
     4381 ttgatttagc aaatgggctg gctttaaata tatcggtaaa tttttgggaa ggagcttagg
     4441 atcttgattg gtcgtaaaaa gtattggtga tgctttagtc ctgattcgct cttcaataat
     4501 ttctatactg ctaagcttat ttttttcgga atcatcataa tagaggagtg cttttcatca
     4561 tgaattaccc atccagaact tctcttttaa atttttttag aatgaatgcc cttaaatatt
     4621 caattcattc agtagtccaa cttttaagga gtattatatt cttcgttcat ttaccaagcg
     4681 tagcattaat tcatcttttc ccgggtttga gttcatgcaa aactgattac ctttcaataa
     4741 agttttttcg gttgatggtt ttatagtaag ttaaggtaat ccaccggatt gtctctatcc
     4801 aacaaacact cctatcgatg cggaatatct ttgtatcaaa attgaatctc tcaattttcg
     4861 tgcattttca ttatttttac aaaatcaatt ttgacagcac ctccatttat atttcaaaaa
     4921 aatgtaattg tatgaaagat gtgatgagtt ttttactttt tttaacattc tctatgttgg
     4981 ggaatattgg tatggcacaa catggtaaag tactgatgct tgatggggat aatgattata
     5041 tgtcggtagc cgatcacgaa gacctggata tcaattcagg tgaaagtttt tccatcacat
     5101 gctgggtaaa aacctttgaa tctgctgatt tttaccggat tgcatccaag cgttctaatg
     5161 ctgcaggtgg ttccgttggg tacgagatga ttaccaataa gtcaggaggc cagtttggaa
     5221 tcaatttgag aagtgcagca ggggttaatg caggtcctcc tttcggggag atacctgtca
     5281 ccgatggcaa atggcatcat ctggcgttga ttttcgatgc tcaatctaag gcttcaagaa
     5341 tctatgtaga tggcaatttg gataatttcc tgaactcgaa tgtgatagga gttcaggagt
     5401 tcagtaatga tgtggctttg ttgttcggaa cttcaattgg tcttaccgga ttttggaatg
     5461 gttggatgga tgagatacgt atctggtcga aggcgcttac tatatctgag ataaaagatg
     5521 atatgcctgc tatcttgaat ggacccgtgg acaatttgat tgctgcctgg gattttgaga
     5581 ctgtgcaaaa tggtatagta ccggacatca cgggtaatca tcccggacaa ttgatagggg
     5641 atgcaaagac tgtagatgat aatagtggta gtgccacaat tacagctgtc acaagatatc
     5701 aatcggctat tccaatcggg cagggcaata aagatgagcg gttggcttca gctaatttta
     5761 aggtatcagg tgaaggaaat aatctggttg tggaatatct gcgtttcaag gttgaatcgg
     5821 atgcaaatac tattggacga tgtaaattgt attataatgg aattaatgaa cggctgaacc
     5881 ttgtcacggc agaattgctg ggggaagctg atgtgtcagg tggctacgtt gaattctctt
     5941 tcaataagac tctggtcgaa ggcaacaatg ttttttggct ttgtggggat atcccgggga
     6001 gtgcatctga aggagcacaa attggctcct ggttgtcctt ccatactgtt tcaggtattg
     6061 aagctgtcat ggatgatcct gcaactccgg ttttcaggac cgtattactt cagcaccagc
     6121 tgttgttttc aggaggagat tttggttcgg ctacttatcg cattccggcc attgcttcca
     6181 ggggtgatcg gattgtggtt gttgcggatg ccaggatcaa caacaatgga gacttgccta
     6241 acaacattga cttattcgcc cgatacagtg aggatggtgg caagacctgg tcagggccgg
     6301 taacggttgc tgattttgga gatagtggcg ccagtgatcc ggcatt
//
LOCUS       DAOQQN010000177         6302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5295_length_6302_cov_4.883144, whole genome shotgun sequence.
ACCESSION   DAOQQN010000177 DAOQQN010000000
VERSION     DAOQQN010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5295_length_6302_cov_4.883144"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLG24_07605"
     CDS             complement(<1..185)
                     /locus_tag="PLG24_07605"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HMZ24792.1"
                     /translation="MKTERLKIAILSGAGISAESGISTFRDQNGLWENHDIMEVASIT
                     GWIKNPALVLDFYNKRRA"
     gene            complement(182..1693)
                     /locus_tag="PLG24_07610"
     CDS             complement(182..1693)
                     /locus_tag="PLG24_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HMZ24793.1"
                     /translation="MLKILNKSQIAHLENEVKISRHLTESQLVSQAGKAFYHEFTKII
                     KDIDLTILILCGNGNNGADGLEIANLLSSAGYNVEVGVILIAKKSSDTFRHKLENLFS
                     SIPTIELDVQDLHDFTLPFYDVLIDGILGSGINRELPTELQSLITRINQNKQPVFSID
                     IASGLSCEGTLPKVAIKPHYTIAMGTIKPAYFLAEGVDHVGILIFARIQSFEECLSQI
                     KSHEFWINPSSAKKILKPYNRLDHKYIHGIASIIGGSYGMSGCIKLSGEAALRSGCGV
                     VYLHVPYNSVDFLQSSLPEAIIHPDDFKYHIGSFENNMRFTAIGIGPGMGYKIKQSEM
                     FGGLLDDNKTIPMVIDADALTILSGWDDWPRRINCPVILTPHKGEFERLFGKFETQQQ
                     RVDAMRNHSTKYGHVILLKGADTVISGPDGSVYFSNTGNSGMATAGSGDVLTGILTGL
                     LAQGYSPIDTAILGSQIHGLSGNIAEANWGKRSIIAGDLVKNIGLAIQSIENQ"
     gene            1778..2806
                     /locus_tag="PLG24_07615"
     CDS             1778..2806
                     /locus_tag="PLG24_07615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMZ24794.1"
                     /translation="MEQKCLPKLFGVILFLSIYTLGFSQNEIHYSQFYNSTLNLNPAL
                     TGIFNGDKRISANYKSQWRSVPVNYFTLSGNYDQKIYSNKNEKIFFGAGANFNFDQAG
                     DGKFGLLNLNFLGSFTYMINRHFFVTPGLGIGFGQRSINSAGLNFGDQWRGDYFDKNP
                     TSGEKVDLDKMSFLYPETSLGLNIRLQNSARTKLDLGGGFCHLNSPKIQFYDGSDSKL
                     PVTMSFYGLGSIYIAQPFDLILNAAYRKSDTYNETLLGAALKVYLSTKRGKEFAVVGG
                     INLRTKDAWVPTIELHVNQWRAGISYDINNSPFEIATEKKGGLEVSLQYIITEVKPLR
                     DYRSCPVY"
     gene            2835..5246
                     /locus_tag="PLG24_07620"
     CDS             2835..5246
                     /locus_tag="PLG24_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24795.1"
                     /translation="MRYIFLMVICCGLMMGTMSAQTKKQYLKAAEKNLSMKDYKSAMV
                     NYQIASEFDPGNADILFNIAEAARKFHAFTKAEQYYALLLLNENKDDPKYKDVPFHLA
                     EVQTIQGKYQEAQDNFNLFISKNDGSDNPNLDVARKRLASLDFAIKNSSDSAQYYKVV
                     QLGSDINSPYNDFAPFEKNDSLFYASNRFTNPTNKWLVGKTMLKDASSNAELGSEFNE
                     NTKHNVNMTYSPDGKRVYYSNCSNLNFSEIRCELYYRNINPDGTFGPAVRLPDPVNMA
                     GYTTTQPSIGYVAGMHKTVLFFVSDRPGGEGKDDIWCIDVNGDSFGTPYNLKDLNTAE
                     SEVTPYFHEGTQTLYFSSFGLNGFGGYDIYRSTYENGKWNAAIQEPAPLNSSYNDIYF
                     SLNKSGKTGYFASNREGSLYLDPTEEACCNDIYKVDVLKVNLLAYVFDASTRDSLPGA
                     KVSLYKKGGPEMVSQQIDTRSSYLFGLDPGFEYMAIATKPGYYPDTVYFTTKDLKDNS
                     DIVKLFYLKSKELDLDVLTFAKKSGLPLNGTTIRIIDMDDPSKEQLIQLTESTNRHVF
                     PIERGKRYKIIATKKGFAPDTVMLNTKDIADNVNRIEQKLYLGKGSLEDYLPIELFFD
                     NDEPGRRSRAVSVTKTFPETFAPYYNKKWLYRDRYIQPLSGDERIQAEKIMNNFFDNR
                     VKKGNDDLIDFTEALEEVLERGEEVQIIIQGFTSPLASTAYNEKLGLRRVSSVVNYLK
                     TASNGAIAPYLKSGKLKITEVSFGETKSPADVVSSYSDLRNSVYSVSASKERRVEIVN
                     INRKK"
     gene            5280..>6302
                     /locus_tag="PLG24_07625"
     CDS             5280..>6302
                     /locus_tag="PLG24_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24796.1"
                     /translation="MYKYLMIKNNNTSHKTFIAGIGKLVVAIILFMVGSFQAHATHIV
                     GGEVTYTCLGNNKYRITLTVYRDCFYADPNVTFDNPAWLGFYSTKSKTLVSNVGALGV
                     VNIPYDATDTLDQILTSECNIEGQDVCVHRAVYDTVVTLPYLVGGYTIVYQRCCRNQT
                     LMNIDEPLNTGAIFSVEITDEALLACNSSPRYEFWPPIYVCAGTPLNYDHGAIDNDGD
                     SIVYRVCNPFVSGDTAEGRIYPPPGPPFDTVTWANGFGLHNLLGGPDPLKINPSTGFI
                     TGTPVIIGQFLVGICAEEYRNGVLLSRIRRDFQYNVRNCSNPTEACFKIPDTLCNTTV
                     IPFINCS"
BASE COUNT         1899 a         1167 c         1296 g         1940 t
ORIGIN      
        1 gcgcgacgtt tattataaaa atcaagtacc agagcaggat tctttatcca gccggttatg
       61 ctggctactt ccatgatatc atgattttcc cacagtccgt tttggtctct gaaggttgat
      121 atgccgctct cagcgcttat tcctgctccg gagagtatag ctattttcag tcgctctgtt
      181 ttcattgatt ttcgatggat tgaatggcaa gtccgatatt tttgacaagg tcacctgcga
      241 tgattgatcg ctttccccaa ttggcctcgg caatattgcc ggacaacccg tgtatttgac
      301 taccaagaat tgctgtgtct atagggctgt atccctgggc caataacccg gtcagaattc
      361 cggtcaggac atcgccggat ccggcagtgg ccataccgct attgccggtg ttggaaaagt
      421 atacagaacc atccgggccg gatattaccg tatcggctcc tttaaggagt ataacatgac
      481 catatttggt tgaatgattg cgcattgcat caaccctctg ttgttgtgtt tcaaatttac
      541 caaacaaacg ttcgaactca cctttatgag gagtaagaat gacagggcag tttattctcc
      601 ggggccagtc gtcccagcct gatagaatgg tcaaggcatc agcatctata accatcggaa
      661 tggtcttgtt gtcatcaagc agtcccccaa acatttccga ttgttttatt ttgtagccca
      721 tgcctggtcc aattcctatt gctgtaaaac gcatgttgtt ttcaaaggaa ccgatatgat
      781 atttaaaatc atcaggatgg ataatggcct ccggaaggga tgattgcaga aagtcaacag
      841 agttgtaagg tacatggaga tatacaacac cacaccctga acgcagggcg gcctctcctg
      901 aaagtttaat gcagccggac atgccataac ttccaccgat aattgaagca ataccatgta
      961 tgtatttgtg atccagtcta ttatatggtt tcaatatttt tttagcactg gagggattta
     1021 tccaaaattc atgagatttg atctggctaa gacattcctc gaacgattga atcctggcga
     1081 agatcaatat gccgacatga tctacgcctt cagccagaaa ataggcgggc tttattgttc
     1141 ccatggcgat ggtataatga ggctttatcg caaccttggg taaagtacct tcacagctta
     1201 aaccactggc aatatcaatg gaaaaaacag gttgtttatt ttgatttatt ctggtaataa
     1261 gtgattgtaa ttcagttgga agttctctgt taatacctga acctaagatt ccgtctatca
     1321 aaacatcgta aaagggtaga gtgaaatcat gtaggtcctg tacatcaagt tcaatggtag
     1381 gaattgaact gaacaggttt tcgagcttat gcctgaatgt atccgatgat tttttagcaa
     1441 taaggattac accgacttca acattatacc ctgcactgga tagtaagttg gcaatttcaa
     1501 gaccatcagc accattattt ccgtttccgc acaaaatcag aattgtaagg tcaatgtcct
     1561 taataatttt ggtaaactca tggtaaaatg ccttaccggc ttgagaaacc agttgacttt
     1621 cagttaaatg tctggaaatt ttaacttcat tctccaggtg tgcaatttgt gatttgttaa
     1681 gaatcttcaa catatatata tcattttttg cattgaattt atataaaagt agtaattttg
     1741 atgaaagata atttgtaaac ccataaacac atttattttg gaacaaaaat gtttacctaa
     1801 actatttggt gttatacttt tcctgagtat ttatacgctt gggttcagcc aaaatgagat
     1861 acattattct cagttttata attcaacact aaatttaaat ccggctctca ccggaatttt
     1921 caacggagat aaaagaattt ccgctaatta caagagccag tggagatcgg tcccggtcaa
     1981 ttattttacc ttgagtggca attatgacca aaaaatttac tccaataaga atgaaaaaat
     2041 cttctttgga gcgggtgcca attttaattt tgatcaggct ggtgacggga agtttggtct
     2101 attgaatttg aatttccttg gttcatttac ttacatgatc aatcggcatt tctttgttac
     2161 accaggtctg ggaattggct ttggacaaag gagtatcaac tctgccggtt tgaattttgg
     2221 tgatcaatgg cgaggtgatt atttcgataa aaatcctact tcaggcgaga aagttgattt
     2281 ggataagatg tcatttcttt atcctgagac ttctctgggt ttgaacatcc ggctccagaa
     2341 cagtgccagg actaaattgg acctcggagg aggcttttgt catctgaatt ctcctaaaat
     2401 tcaattttac gatggaagcg attccaagtt gccggttaca atgtcatttt atggacttgg
     2461 ttccatatat attgctcagc catttgattt gatattgaat gcagcttata gaaaatcgga
     2521 tacttataac gaaactctat tgggtgcagc attgaaggtt tatttaagta ccaagagagg
     2581 taaagaattt gctgttgtag gcggaatcaa cctccgaaca aaagatgcat gggtgcctac
     2641 cattgaactt catgtcaacc agtggcgtgc aggtataagt tatgatatca ataactctcc
     2701 tttcgaaata gcaaccgaga aaaaaggcgg attggaagtt tcattgcagt atattattac
     2761 agaagtgaaa ccactgcgag attacagatc ttgcccggtc tattaaacaa tagtaatcct
     2821 aaagaataaa aactatgaga tatattttct taatggtcat atgctgcggg ctgatgatgg
     2881 gtaccatgtc ggctcagacc aaaaagcagt atttaaaggc agccgagaag aatcttagca
     2941 tgaaagatta taaaagtgct atggtcaatt atcaaattgc ctctgagttt gatcccggaa
     3001 atgccgatat tttattcaat attgctgaag ctgcaagaaa gtttcatgca tttaccaaag
     3061 cagaacagta ttatgcatta ctgctattga atgagaataa ggatgatcct aaatataagg
     3121 atgtcccttt tcatcttgct gaagttcaaa ccatacaggg taaatatcag gaagcacagg
     3181 ataatttcaa tttattcatt tcgaagaatg atggttcaga taatcccaac cttgatgtgg
     3241 cacgtaaaag attagcatcc ctggattttg ctataaagaa ttcttccgac tcagctcaat
     3301 attataaagt ggttcaattg ggtagtgaca tcaattcacc atacaacgat tttgcccctt
     3361 ttgaaaaaaa tgactctctc ttttatgctt caaaccgatt taccaatcct acaaataaat
     3421 ggttggtagg gaagacaatg ctaaaggacg cgtctagtaa cgctgagttg gggtctgagt
     3481 ttaatgaaaa taccaagcat aatgtcaata tgacctattc tccggacggg aagagagtct
     3541 attattccaa ttgttctaat ttgaattttt cagagattcg ctgcgaattg tattatagga
     3601 atattaatcc tgatggtact tttggaccgg cagtcagact tcctgatcct gtcaatatgg
     3661 ctggttatac cactacacaa ccaagtattg gttatgtagc cggaatgcat aagacggtat
     3721 tgttttttgt ttcagacagg cccggtggag aaggtaaaga tgatatatgg tgtattgatg
     3781 tcaatggtga ttcctttggc acaccatata atttgaaaga tttgaatacg gcagaatcgg
     3841 aagttacacc atattttcat gaaggtaccc agacacttta tttttcatcg tttggcttga
     3901 atggttttgg tggttacgat atttatagat ctacctatga aaatggtaaa tggaatgccg
     3961 caattcaaga acctgcacct ttgaacagta gttacaacga tatttacttc agtctcaaca
     4021 aaagtggtaa aaccggttat tttgcttcta accgcgaagg ttcactttat ttggacccta
     4081 cagaagaggc atgttgtaat gacatctata aggttgatgt cttgaaggtc aacctacttg
     4141 cctatgtatt tgatgcttca acgcgtgatt cattgcccgg agccaaggtg agtttgtata
     4201 agaaaggcgg tccggaaatg gtaagccagc aaatcgatac acgatcatcc tatctttttg
     4261 ggcttgatcc cggatttgaa tacatggcta tagctaccaa acccggctat tatcctgata
     4321 ctgtatactt tacaactaaa gacctgaaag acaattcaga tatagtgaag ttgttctatc
     4381 tcaaatcaaa agaactcgat cttgatgtac tcacttttgc taaaaagtcc ggattgccac
     4441 tcaatggaac gaccatcagg attattgata tggatgatcc ttcaaaggaa cagctcatac
     4501 agttgaccga gtctactaac cgtcatgttt tcccgataga aagaggtaag agatataaaa
     4561 tcattgctac aaagaaagga tttgcacctg acactgtcat gttgaatacc aaagatatag
     4621 cagataatgt aaatcgtatt gaacaaaagt tatatctcgg aaagggaagt ctcgaggatt
     4681 atcttccaat cgaattattc ttcgataatg acgaaccagg aaggagatcc agagctgtgt
     4741 cagtaacgaa gaccttcccg gaaacttttg cgccatatta taataaaaaa tggctttatc
     4801 gtgatcgtta catccaacct ttatccggcg atgaaaggat tcaggctgaa aagattatga
     4861 ataatttctt tgacaataga gttaagaaag gaaatgacga tctgattgat tttacagagg
     4921 cacttgagga agtattggaa aggggagaag aagtccaaat catcattcag ggctttacct
     4981 ctccattagc ttcgacagca tataatgaaa aacttggact cagaagggta agcagcgtgg
     5041 ttaattattt gaaaacggct tcaaatggtg cgattgctcc atatttgaaa agtggaaaat
     5101 tgaaaattac tgaggtttca tttggtgaga caaaatctcc tgctgacgta gtttcaagtt
     5161 acagtgattt aaggaattca gtttatagcg tctctgcatc caaggaaagg cgtgttgaga
     5221 ttgtcaatat taacagaaag aagtaatccc atatttattc aataaaagta ataatatcca
     5281 tgtacaaata tttaatgata aaaaataata atacttcgca taaaaccttt attgcaggta
     5341 taggcaagct ggtcgtagcg attatattat tcatggtcgg ttcctttcag gcccatgcta
     5401 cccatatcgt tggaggggaa gtcacatata cttgtctggg aaataataaa taccggatca
     5461 cactgacggt ttatagagac tgtttttacg ctgaccccaa cgtaaccttt gataatcctg
     5521 catggcttgg attttatagt acaaaatcca aaaccttagt atctaatgtc ggtgctctgg
     5581 gtgtggtcaa catcccatat gatgctacag atacattaga ccagattctt acttctgaat
     5641 gtaatattga aggtcaggat gtctgtgttc acagagccgt ctatgacact gtcgtaactc
     5701 tcccttattt ggtaggaggt tatacaatcg tctatcagcg ctgttgtcgt aatcagactc
     5761 taatgaatat cgatgaacca ttgaatacag gagctatatt ttctgtagaa attacagatg
     5821 aggctctctt ggcttgtaat agttctccaa gatatgaatt ttggccgcct atctatgttt
     5881 gcgcgggtac accattgaat tacgaccacg gtgcaattga taatgatggg gatagcatag
     5941 tttatagagt atgtaatccg tttgtgtcag gtgatacagc agagggaagg atctatccac
     6001 ctcccggacc accattcgat accgttacat gggctaatgg ttttggactg cataatcttt
     6061 tgggtggtcc tgatccactt aaaattaatc cttcaaccgg ttttattacg ggaactccgg
     6121 taattatagg gcagtttctg gttggtatct gtgctgaaga atacaggaat ggcgtactat
     6181 tatccagaat aagacgagac tttcagtata atgttcgtaa ttgctccaat ccgactgagg
     6241 catgttttaa gattcctgat accctatgta acactactgt aattcctttc attaactgtt
     6301 cc
//
LOCUS       DAOQQN010000178         6234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5354_length_6234_cov_5.556563, whole genome shotgun sequence.
ACCESSION   DAOQQN010000178 DAOQQN010000000
VERSION     DAOQQN010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5354_length_6234_cov_5.556563"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLG24_07630"
     CDS             <1..308
                     /locus_tag="PLG24_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ24797.1"
                     /translation="VGRPKERWEIAISMLKKVGLEEYHARRYPHEFSGGQRQRIGIAR
                     ALALNPKFIVCDEPVSALDVSIQSQVLNILKDLQKEFGLTYLFIAHNLSDYCEPKMS"
     gene            520..1707
                     /locus_tag="PLG24_07635"
     CDS             520..1707
                     /locus_tag="PLG24_07635"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HMZ24798.1"
                     /translation="MKRNFSIYLLLAFMLGGFCLNAQDDLINKIKGNQSANAKFEFTK
                     IIDLENTSIKDQGSSGTCWSYSTVSFLESEMARIGKKPVDLAEMFIVRNQYIQKAENY
                     VRMHGGLLYGQGGAAHDVIKIYGQFGIVPQNVYTGLNYGTTRNNFNELEAVLKGFLAP
                     LVGDNVKMLTPVWKTAFTRIVDTYLGEVPEKFMYEGKEYTPKTFADQVVGLKAEDYVE
                     FSSFTTSPFYKPTVLMVPDNWSFDMVYNVPIDDLTAIVDYALKNGYTITWGTDVSDRG
                     FSWKNGVAYVLPMDYDAMSEEQKKDIFNGPQPEGKVTQEERQRQFDNYQTTDDHGMQI
                     VGLYKDQNGREYYKVKNSWGQRNDFDGYLFVTKNFLKLKTTDLLLHKGGVPKDLLKKC
                     GIQ"
     gene            1860..2513
                     /locus_tag="PLG24_07640"
     CDS             1860..2513
                     /locus_tag="PLG24_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24799.1"
                     /translation="MSSTIIRYSDEELEEFRVLIEEKLSVARNELEFMKEQITEMNEN
                     ASEKQGGNWFDDSSIHTELEMLNNMVIRQQQFIHNLENALIRIQNKTYGICSVTGQLI
                     DKNRLKLVPHATKSVVAKEIENAEKAKQEAIARAEKEAAKASEKSGESADVKPKIITK
                     IVKRSNKPATKTAPKEEDLEDLDLDLDLEEEESDYDDTLINGMDMDDIADDQDYFDK"
     gene            2697..2852
                     /gene="rpmH"
                     /locus_tag="PLG24_07645"
     CDS             2697..2852
                     /gene="rpmH"
                     /locus_tag="PLG24_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HMZ24800.1"
                     /translation="MKRTFQPSRRKRVNKHGFRARMSTKNGRRVLASRRAKGRHKLTV
                     SDEMKTK"
     gene            2921..3301
                     /gene="rnpA"
                     /locus_tag="PLG24_07650"
     CDS             2921..3301
                     /gene="rnpA"
                     /locus_tag="PLG24_07650"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HMZ24801.1"
                     /translation="MKRPFSFPKSQRLKNPLSISILFQSGDNVFSYPVKMVFSFEADP
                     VCHPSFKAAVSVPKKSFKKAVDRNRLKRHMREAIRLNQSLIPVREGHTLRFIIIYAAR
                     EVLPAQQIHKAVRYLFEKLNKDEN"
     gene            3291..3848
                     /locus_tag="PLG24_07655"
     CDS             3291..3848
                     /locus_tag="PLG24_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24802.1"
                     /translation="MRTNIYLIIDLLFFRLVVCAIFLFSMNNVMGQRYLQVEKHHKKV
                     KLKYTAGDDITFRLRQSDQWLTSVLTDIDFDNNILFFTNLTVPLDSINGIKSHKPLVA
                     PELGMAVLRSGLSGLGTSLLYTLAFQPSNAKEFLLFFGGVSASGLGLTQLSKKRRVFD
                     IGNKFNLRITDLNIYIPQSPPVPGG"
     gene            3930..4541
                     /locus_tag="PLG24_07660"
     CDS             3930..4541
                     /locus_tag="PLG24_07660"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMZ24803.1"
                     /translation="MSKFIGISIFLVPLVFSILAFRTYDNGAYTIGDTVEDFTLTNYD
                     GSMISLSGYNNAKGLIIVFTGNQCPFDQAYESRLIDLDRKYRADGFILLAINPNDAGM
                     SAVDNLEAIRARAQQKSFTFPFLFDESGSLRRKFGVVRSPHAFVLDGARKIVYSGAID
                     DSVTEPEKIRSRYVEEAIIALKEGKSIVKPLTKPVGCTVKADF"
     gene            complement(4547..5356)
                     /locus_tag="PLG24_07665"
     CDS             complement(4547..5356)
                     /locus_tag="PLG24_07665"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMZ24804.1"
                     /translation="MENQDNYLEINRRLWDAKTAYHLNSEFYDVKTFLDGRNSLTEIE
                     LALLGDISGRSVLHLQCHFGQDTISLSRMGASATGVDFSSKAIETARQLAQESGSTAQ
                     FICSDIYDLPNQMDKKFDLVFTSFGVIGWLPDLKKWAEVIAHFLKPGGKFIFAEFHPV
                     AWMFDNNFSSIQYNYFNVEAIREEAVGTYADRDAPIRNESITWNHSLSETLNALIESG
                     LEIKHFEEFDFSPFNCFNEMILSDDQKFRIRQLDNKIPLTFALTAVKKIRN"
     gene            5715..5957
                     /locus_tag="PLG24_07670"
     CDS             5715..5957
                     /locus_tag="PLG24_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24805.1"
                     /translation="MISRNEGHQLLIFKAFSLVFVCTGPLLGVPFQINDLSSYKDLGR
                     QSSLKNLEEVYMLRFVYRPAYPGGTRLFIPIVVAVN"
BASE COUNT         1890 a         1143 c         1301 g         1900 t
ORIGIN      
        1 acgtggggcg ccccaaggaa cgctgggaaa tcgccatcag tatgttgaag aaggtggggc
       61 ttgaagaata ccatgcccgc cgctatccgc atgagttttc tggcggtcaa cgtcagcgca
      121 tcggcattgc ccgggctctg gctttgaacc ccaaatttat cgtttgcgat gaaccggttt
      181 ctgcgcttga tgtttccatt caatcacagg tattgaacat cctcaaagac ctgcaaaaag
      241 aattcgggct gacctacctt ttcattgctc ataacctcag tgattattgt gaacctaaaa
      301 tgtcataaat tttaaatcga tgctgttatt tttaaagaat ttctctgttg gcaatctact
      361 gacatcacta tggaatggag aaattaacaa ttaagtttgt aattttttct gtacttttat
      421 ttatacttat taatattgca ccgaattttc aaaatgtgag tttgattatt cggaaaatta
      481 tcgtttgtcc aatcggataa taacctataa aacattataa tgaagagaaa tttttccatt
      541 taccttttgc tggcattcat gttgggggga ttctgcttaa atgcacaaga tgacctgatc
      601 aataagatca aggggaacca aagtgccaat gccaaatttg aatttactaa gattatcgat
      661 ctggaaaata cttccattaa ggatcagggt tcatccggaa catgttggag ttattcaacg
      721 gtttcttttt tagagtcgga aatggccagg ataggtaaaa agccggttga cttggcagaa
      781 atgtttattg tacgcaatca gtacattcaa aaagctgaaa attatgtgag gatgcatggt
      841 gggctgcttt acggtcaggg aggtgctgca catgacgtta tcaagatata cggccagttt
      901 ggtattgtac cacaaaatgt atatacaggt ttgaattatg gtacaaccag aaataacttc
      961 aatgaactgg aagcagtact gaagggattt ctggctcctc ttgttggtga caatgtgaaa
     1021 atgcttactc cggtgtggaa gacggccttc accagaattg tagatactta cttgggagaa
     1081 gttcctgaga aatttatgta tgaaggtaag gaatatactc ccaaaacctt tgcagatcag
     1141 gtcgtgggct tgaaggcgga ggattatgtc gaattttcat cgtttactac gagccctttt
     1201 tataagccta ctgtattaat ggttcctgac aactggagct tcgacatggt gtacaatgtg
     1261 cctatcgatg acttgacagc catcgttgat tatgccttga agaatggata taccataaca
     1321 tggggtactg atgtcagtga ccggggtttt tcatggaaaa atggcgtggc atatgtgttg
     1381 cctatggatt atgatgcaat gagtgaagaa cagaaaaagg atatttttaa tggcccgcag
     1441 cctgagggaa aggttacaca agaagagcgt caaaggcagt ttgataatta tcaaacgacc
     1501 gatgaccatg gcatgcagat agtagggtta tacaaagacc agaatggcag ggaatattat
     1561 aaagtaaaga atagctgggg acagagaaat gattttgacg gatatctttt tgttaccaag
     1621 aattttttga aactaaaaac cacagatttg cttttgcata aagggggagt tcccaaagat
     1681 ttacttaaga aatgcggtat tcagtagaat aaatataatg atatgtgtat ctttcgttgt
     1741 atttatcaaa aatgatactt tcaattaaga atgaaaattt aatgaactta taagacgtaa
     1801 aatacgtttt tgtctttatc cgaaactgag attgaataaa ttgttaaaaa ccaaacatta
     1861 tgtcatctac cattatacga tattctgatg aggagcttga agaattcaga gtattgattg
     1921 aagagaagtt atcagttgcc agaaatgaac ttgaattcat gaaggagcag ataactgaaa
     1981 tgaatgaaaa tgccagtgaa aaacaagggg gtaactggtt tgatgattcc agtattcata
     2041 cggaactgga aatgctgaat aatatggtga ttcgtcagca gcagtttata cataatcttg
     2101 aaaatgccct gatacgaatc cagaacaaaa cgtatggtat ctgctcggtg accggtcaac
     2161 taatcgataa aaacaggctg aagttggttc ctcatgctac gaagtctgta gtggccaaag
     2221 agatcgaaaa tgcagaaaag gccaagcagg aggctatagc cagagctgag aaagaagcag
     2281 caaaggcttc tgagaaaagt ggagagtctg ctgatgtgaa accgaagata attacaaaga
     2341 ttgtcaagag atccaataag cctgctacca aaactgcgcc taaagaagaa gatttggagg
     2401 atctggactt ggatcttgat ttggaagaag aagaaagcga ctatgatgat actttgatca
     2461 atggtatgga catggacgat atcgcggatg atcaggacta ttttgataaa taatagagag
     2521 caatcacata caaaagccct acatttcgtg gggcttttta ctttgtgaat agttcgtccg
     2581 atcatcctcc ggaacaatgt tgcttaaaaa agtaataaaa aaatggattt aaagtttttt
     2641 taatcaaagt ctatttaata cctttgtccc actttttgaa aaaataacca tattctatga
     2701 aacggacgtt ccagccttca aggcgtaaaa gagtaaataa gcacggattc agagccagaa
     2761 tgagcactaa aaatggtaga agagtgctgg caagcagaag agctaaagga agacataaat
     2821 tgactgtttc tgacgagatg aagacaaagt aagttattcc ttataaaaac tattgatagg
     2881 ctattgcgca agtcagcaca atagcctatt tttattttca gtgaaaagac cattttcatt
     2941 tcctaagagt caaaggctta aaaacccttt gtccatttcg attcttttcc agtcaggcga
     3001 caacgttttc tcctacccgg tcaagatggt gttttctttt gaagcagatc ccgtctgcca
     3061 tccctccttc aaggcagcag tttcagtgcc aaagaaatct ttcaaaaaag cggttgacag
     3121 gaatagactg aaaagacata tgcgtgaagc cattcgcctg aatcagtccc tgattccggt
     3181 tagagaaggc catacactcc gcttcattat tatttatgct gccagagagg tcctcccggc
     3241 tcagcaaatc cataaggcag tgcgttattt atttgagaaa ctgaataaag atgagaacta
     3301 acatatactt gattattgat ttgctgtttt ttcggttggt agtttgtgct attttccttt
     3361 tctcaatgaa caatgtaatg gggcaaaggt atcttcaggt tgaaaagcat cataaaaagg
     3421 taaaactcaa atatacagca ggggatgaca ttacattccg cctccgacaa agcgaccaat
     3481 ggcttacttc agttctcacg gatatcgact ttgataataa tatccttttc tttacaaatc
     3541 tgactgtgcc gttggactct atcaatggta tcaaaagcca caaaccactc gtggcgcctg
     3601 aactcggtat ggctgtattg cggtcaggct tgtcaggcct gggaacatcc ttgctttata
     3661 cccttgcttt tcaaccatca aatgctaagg aattcctgct ttttttcgga ggtgtatccg
     3721 ccagcggcct tggtttaaca cagttatcca aaaagagaag agtatttgat attggaaaca
     3781 aatttaatct tcggatcacc gatctcaaca tctacatacc tcaatcgccg ccggttcccg
     3841 gggggtaaat tttctttcga gaacttgatt atagttttat ttgttgagtt atcatgggtt
     3901 ttaatgttaa aaatagaaat attaattcga tgtctaagtt tatcggcata tctatctttt
     3961 tggttccgct ggttttttcc attttggctt tccggaccta tgacaatggc gcttatacta
     4021 ttggggatac tgtggaagat ttcaccctta cgaattatga tggcagcatg atttcactat
     4081 ctggttataa taatgctaag gggcttatca ttgtttttac aggcaatcag tgtccttttg
     4141 accaggcgta tgagagcaga ttaattgacc tggatagaaa gtatcgcgcc gatggtttca
     4201 tactgttggc tattaatccg aatgatgcgg gtatgtcagc tgtggacaac cttgaagcga
     4261 tccgtgccag agcacaacag aaatcattta cctttccttt tctttttgat gaaagcggaa
     4321 gtctacgccg aaaattcgga gtagtaaggt ctccgcatgc ctttgtgctg gatggtgccc
     4381 gtaaaattgt ttattccggc gccattgatg acagtgtcac tgaacctgaa aaaatcagga
     4441 gtcgatatgt tgaagaggcc attattgctc tgaaagaggg aaaaagtatt gtcaaaccgt
     4501 tgaccaaacc tgttggatgt actgtcaaag ctgattttta aaccccttag tttcttattt
     4561 tttttacggc agtcaaagca aaggtaagtg ggattttgtt gtccagttgc ctaatacgga
     4621 atttttgatc atccgataat atcatttcat tgaagcaatt gaatggcgaa aaatcaaatt
     4681 cttcgaaatg tttgatttca agaccacttt caatcagagc gttgagtgtt tcactcaaac
     4741 tatgattcca ggttatactc tcgttccgga tgggcgcgtc cctatcggca taggtgccga
     4801 ctgcttcttc ccggattgct tccacattaa aataattgta ttgaatactt gaaaaattat
     4861 tgtcaaacat ccatgcaacc ggatgaaatt cggcaaaaat gaattttccg ccaggcttca
     4921 aaaaatgagc tatgacttca gcccattttt taagatcagg tagccatcca atcacgccga
     4981 aactcgtaaa tacaagatca aactttttgt ccatttgatt gggcaaatca tagatatctg
     5041 aacaaatgaa ttgagcagtc gaacctgact cttgtgccaa ttgtcttgct gtttcaatgg
     5101 ctttggagga aaaatcaact ccggtagcag aagcccccat tctgctcaat gagatggtat
     5161 cctgaccgaa atgacactga agatgcaata cactcctacc gcttatatct cccagcaatg
     5221 ccagctcaat ctcagtcagc gaattccttc catcaagaaa tgtcttgaca tcgtagaatt
     5281 cagagttaag atggtacgcg gtcttggcat cccacaatct tcggttgatt tccaaataat
     5341 tatcttgatt ttccataatg actttatttg attttttttc tgagcactaa cttaccggct
     5401 caataattaa tacgtttgtt ttttacccga aggatttaat ttttttagtt taggaatgaa
     5461 ataaagtgcg ccattacaag ctgataggag attttttacg ctcacacttc atgacatcac
     5521 aaccatcgat tgaaaaaggc atatttacac tgagtatatg gaaataaatc ttttatcgtg
     5581 attagtcctg caaagaaaag tatttaattg gttatccatc aaaaaatgtt tgatctgcca
     5641 tcatcagtac ttgcctttgc caaatcgtaa ttgtttcaat catcatatcc tttgatttgg
     5701 atgaaccctg gctcatgatc tccagaaatg aaggccatca gttactaata ttcaaagcct
     5761 tcagtctggt ttttgtgtgt acaggaccct tattgggcgt tccattccaa atcaacgact
     5821 tatcttcgta taaagacctg ggccggcaat cgtccttaaa aaatttagag gaagtatata
     5881 tgctccgatt tgtctatagg cctgcatatc cgggtggaac ccggcttttt atccctattg
     5941 ttgtagctgt caattgattt gcttcaaaaa atgtcataga atggactccc atgattttga
     6001 gcattaattt tgtctgttaa gctgcttttt ttgaaatgct tttccggaga attcaattta
     6061 ccggtacccg ggttaatagg ttggccccgg attttagaaa agccggattt tcagatctgg
     6121 ttgatggtaa tcgttgttat accaagatga tttatataat agttcataaa tttttggtat
     6181 aattgtccgg acagctatgc taccagagct ctgctctgtc ccgggagtga aaca
//
LOCUS       DAOQQN010000179         6231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5358_length_6231_cov_5.073672, whole genome shotgun sequence.
ACCESSION   DAOQQN010000179 DAOQQN010000000
VERSION     DAOQQN010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5358_length_6231_cov_5.073672"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PLG24_07675"
     CDS             complement(<1..423)
                     /locus_tag="PLG24_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMZ24806.1"
                     /translation="MTYVLHFKNGRIKREGRGLSFFYFAPNSSIAAIPMGSNDLPFIF
                     NESTNDYQTVNIQGQISYQISNPKTLADILDFTVNDNGQYKKNDIEKLNQRIINEAQT
                     ATSSFIHEIKLKDAIRSAKSIEEKILEGLRSSQAIAMLG"
     gene            1581..4019
                     /gene="pheT"
                     /locus_tag="PLG24_07680"
     CDS             1581..4019
                     /gene="pheT"
                     /locus_tag="PLG24_07680"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HMZ24807.1"
                     /translation="MRISHNWLKELLPHQYKTEEIAETLTDIGLEVEGVEVVESIRGG
                     LKGVVIGKVLTRAQHPNADRLSVTTVDIGGDTPLNIVCGAPNVAAGQKVLVATIGTEL
                     YDHEGKSFTIKEAKVRGELSQGMICAEDELGLGESHEGILVLPEDAVIGMNASEYFQI
                     TSDEVIEIGLTPNRADANSHYGTARDLRAALHLHKGYNQALNRVKTLDTAGLPTSDFK
                     ITIENEVACKRYTGILIEQVKVGPSPDWMKTRLEAIGQRSVNNIVDITNYVMFELGQP
                     LHAFDADKIAGRHIRVKNVAAGTPFLTLDGSSKNLDADDLLICDGNGDPMCIAGVYGG
                     KDSGVTDTTVNIFLESAWFNPASVRRTSFRHNLRTESASHFEKGTDPEQCDEALLRAA
                     YLMKEYAEAEVRFTFLDCYPGKSERTKVNINLPRVRTLIGKHLTAQEISATLTSLNID
                     IEEKDTDNWQVTIPSHKTDVSRQADVVEEIVRIYGLNNIEETGSFNFSMPDKVSNEDI
                     EARHTVLQYLADAGYSEAMGLSIINSNNWAKITDNPDIKGIRINNTSNVQLDLMRPDP
                     LATALETIAYNQSRQQSDIRMYEFGSIYDIDQKGYLEATFISLFGTGSLHEESWHIGK
                     RKNLDPYYFKTTIQHLLKRIGIRKYQERNGTDERFENCLEIVLGPKVLAVTGNVRKKL
                     VKNAGIKGSVVFAHIYWEEVLKLIHQKPIIFKEFSRFPVVRRDLAVVVPSTTDFEQIK
                     TLIQKTAAPYLSDLKLFDVYSDEEQLGANKDSLSIALYFTNYEKTFDDKSIEEIMKKV
                     MKAVEEKLDVLIRK"
     gene            4115..4414
                     /locus_tag="PLG24_07685"
     CDS             4115..4414
                     /locus_tag="PLG24_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24808.1"
                     /translation="MIQILAERLESIDKKLKKLAQKYELVKKENTLLMEENINLKQSA
                     GKHKAELEQVGKNLSESGKDLIKATANAQENEKVRKKLDEYIKEIETCIELLENL"
     gene            4418..4708
                     /locus_tag="PLG24_07690"
     CDS             4418..4708
                     /locus_tag="PLG24_07690"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HMZ24809.1"
                     /translation="MAAEESKQISVIIAGRGYPLKIKEGEEDAIMMIADEVNKRIREY
                     QEVYPNREKQDWLAMAILSYAIERFQSEKAMTDSKLVQRFDEVEHYLDDLLK"
     gene            4803..>6231
                     /gene="rny"
                     /locus_tag="PLG24_07695"
     CDS             4803..>6231
                     /gene="rny"
                     /locus_tag="PLG24_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HMZ24810.1"
                     /translation="MDSTMIIIFGIIGGAAIGFFVGNLILKKRMQNQVDEMNAKADLQ
                     IQEARLTAKRIVDDAEVNAEKIISKAERENEKKKQQKIAEAKENFERLKSQFEGEKDK
                     FYSKLKEREIEVVKKETDIKQTQQNLQQQKENLDSKETELKLIRENLDTQLKIVNRRK
                     DELEASHEAHIKQLESISKLSQQEAKEQLIQSMTAKAENEAMSLVKDTIEQAKQTANK
                     EARKIIIQSIQRMGAEITIENTVTVFNLESDDIKGQIIGREGRNIRAIEAATGVEIVV
                     DDTPEAIILSSFDPIRREIARLSLKKLVTDGRIHPARIEEVVAKTKKQIEEQIIEIGE
                     RTVIDLDIHGLDPYLIKMVGRMRFRSSYGQNLLKHSIETSNLCAIMASELGLNPKQIK
                     MAKRAGLLHDIGKVSEEESELSHAILGMKIAEKHGEVKDVINAIGAHHDEIEMDNIIS
                     PIIQACDAISGARPGARREILESYMK"
BASE COUNT         2095 a         1226 c         1292 g         1618 t
ORIGIN      
        1 tccaagcatt gcaattgcct gtgatgatct aagtccctcc aaaattttct cttctattga
       61 ttttgctgaa cgaatagcgt ctttcaattt tatttcgtga ataaaggaag acgtcgctgt
      121 ctgtgcttca tttattattc tttgattcag tttctcaatg tcatttttct tgtattgccc
      181 gttgtcgtta accgtgaagt ctaaaatatc tgccagagtt ttgggattac ttatctgata
      241 acttatctgt ccttgaatat taacggtttg ataatcgttt gtcgattcat taaatataaa
      301 aggtaaatca ttacttccca tgggaatagc tgcaatagag ctattcgggg caaaatagaa
      361 aaatgaaagt ccacgacctt ctctcttaat tcttccgttc ttgaaatgaa gaacgtatgt
      421 cattgagtca aatttgatgt gttttattcc taacataata ttttgtttct aatgcttaac
      481 atggtttatt atcactagtg tctttatata attcatcatg aatacagctg acacatttta
      541 ttattgttca cagagggaca ttgacagtgt ctttggttta tgctttctaa gctcttccag
      601 ccttcaatac cgaagaaaca ttcgttatag accaatttca ttgatctata cgacagtaac
      661 ttcggcatta taccaaaaat atatggactg ttatataaat catcttggta taacacccga
      721 aaccggaatg taaagctacg gagggatctg tgcactcatg gaaaaggttt cggctgaaaa
      781 agtttttaat tttgatggtt gtgcaattgc cggaagggga actgctctat tgtgtaaaaa
      841 acttttaatg ccttatacct cattaaaaaa tcaaatctct accgttttgc ataatagctt
      901 ttccatttcc cttgaattaa caagcatact ttatatacat cgttgggcat aagccaatta
      961 agcttcggag ggggcgctta taactataaa aatcaaagaa cttctttatt tcagtttttg
     1021 ctcaagcaat attactaaat ttttttgtct ttcattctta aacaattctt ttattgattt
     1081 attgaagcgt tctgccacag cattacccaa agcccccctt ggatagtccg gactatcaaa
     1141 taccggaggg ttgattcctt taacatatac ttttcgggta aaagtatcca actcatgccc
     1201 tgatattgca aatttaatgg taattgcctc aaaaaggatg tactaaggca tttgcttacc
     1261 catctcaagt attctttacc ccggaattat caccaaataa tccgaatcac agtggctcac
     1321 tttactccgg aatatcgccc tacacccaat tccacaatcc taagacatat tttattcaag
     1381 gccttccggc aaggcaacct tcctttcaat tataatatca ctcaagatag ttatgcattt
     1441 atcattgaat atcatttatt tacgtacata agataattga tttcccctct ttggcatcca
     1501 cagccatgtt tgacaggctg aaccatcacc gagtcaacaa aatataattt tacattatct
     1561 ttgggcaatt tttaattgaa atgagaatat cacataactg gcttaaagag ttattgccac
     1621 atcagtacaa aacggaagaa atagctgaaa cccttacgga tattggcttg gaagtggaag
     1681 gagtcgaagt ggtcgaatca atacggggtg gcctcaaagg tgttgttatc ggcaaagtac
     1741 ttaccagagc acaacatccc aatgctgacc gcctttcggt cacaacagtc gatataggag
     1801 gagatacacc cttaaacatt gtatgcggag ctcctaatgt tgccgccggc caaaaggtcc
     1861 tggtcgcaac cattggcaca gaattatacg atcatgaagg caagtcattt acaatcaagg
     1921 aagccaaggt cagaggcgaa ctatctcaag gtatgatttg cgctgaggat gaactcgggt
     1981 taggtgaaag tcacgaaggc atcttggtac ttccggaaga tgccgtaata ggcatgaatg
     2041 cttctgaata tttccagata acttccgacg aagtcataga aattggcctg acacccaacc
     2101 gggctgatgc caactctcat tacggcactg cgagggatct ccgggctgca cttcacctgc
     2161 ataaaggata caatcaagcg ctcaatcgcg tcaaaaccct tgacaccgca ggcctcccca
     2221 cttctgattt taagataacc atcgaaaatg aagttgcttg caagagatat accggaatct
     2281 tgatcgaaca ggtcaaggta ggtccttcgc cggattggat gaaaacaaga ctggaagcca
     2341 ttggtcagcg gtcagtcaac aatatcgtgg acattaccaa ctatgtgatg ttcgaacttg
     2401 gccaaccgct gcacgcattc gatgcagata agatagcagg acggcacata agagtaaaga
     2461 atgttgcagc cggcactccc tttttgaccc ttgatgggag ttcaaaaaat ctggatgccg
     2521 atgacttgtt gatctgtgat ggcaacggtg atcccatgtg tattgcagga gtgtatggtg
     2581 gaaaggattc cggtgtaaca gatacgactg tcaatatttt cctggaatca gcctggttca
     2641 atccggcttc tgtccgccgt acttcgttca gacataacct gagaactgaa tctgcatctc
     2701 atttcgaaaa aggaaccgat cctgaacaat gtgacgaagc attacttaga gccgcatatc
     2761 ttatgaagga atatgctgaa gctgaagtac gttttacgtt tttggattgt tatcccggca
     2821 agtcagagag aacaaaagtc aatatcaatt taccgagagt aaggacactg atcggcaaac
     2881 atttaactgc gcaggaaatc agcgccacac taacatctct gaacatcgat atcgaagaaa
     2941 aagatacaga taactggcag gtaaccattc cttcgcataa gacagatgta agcaggcaag
     3001 ccgatgtcgt agaagaaatt gtcagaattt atggactgaa taatatagaa gaaacaggaa
     3061 gttttaactt ttccatgccg gacaaagtct caaatgaaga tatagaggca aggcacacgg
     3121 tgcttcaata tttggcagat gccggataca gtgaagcaat gggattatcc attatcaaca
     3181 gtaataattg ggcaaaaata accgacaatc cggatattaa gggcatcaga atcaacaata
     3241 cttctaatgt ccaacttgac ctaatgagac ccgaccctct ggcaactgct cttgagacaa
     3301 tcgcatacaa tcaatccaga caacagtcgg atatccggat gtatgaattt ggaagtattt
     3361 atgacatcga ccagaaaggt taccttgagg caacctttat atcgttgttt ggaaccgggt
     3421 ccttgcatga ggaatcctgg catattggca agcgaaaaaa cctggatcct tattatttca
     3481 agacaacgat tcagcatttg ctgaaaagga ttggtatccg gaaatatcag gagagaaacg
     3541 gaaccgatga aaggtttgaa aactgtctcg aaatagtatt gggacccaaa gtactggctg
     3601 taaccggcaa tgtacggaaa aaattggtaa agaatgccgg tatcaaagga tcggtggtat
     3661 ttgcgcatat ttattgggaa gaagtattga agctcataca tcaaaaacct attatattta
     3721 aggaattcag ccgctttcct gtggtaagaa gagatcttgc agtagtcgtc ccttccacta
     3781 ctgacttcga acaaataaaa acgctgattc aaaaaacagc tgctccatat ttatcagatc
     3841 taaaactctt tgatgtgtat tcagatgaag agcagcttgg tgccaacaag gacagcctga
     3901 gcatagcctt gtacttcacg aattatgaga aaacattcga tgataagagc attgaagaga
     3961 tcatgaagaa agtcatgaaa gcagtggaag aaaaacttga tgtattaatt agaaaataaa
     4021 tagtattttt gcaaattgta tgagtgtttt gaaatattat accattaatt aggattaaat
     4081 ttcattacat tgaaaaatat ttttgaggca taaaatgata cagatattag cagaaaggct
     4141 tgaatcgata gacaaaaagc ttaaaaagct tgcccaaaaa tatgagctgg tgaaaaaaga
     4201 aaacaccttg cttatggaag agaatatcaa cctcaagcaa agtgccggaa aacataaagc
     4261 ggagttggaa caagtgggaa agaatctgtc agaatctgga aaagacctga tcaaagccac
     4321 tgcgaatgcg caggaaaatg aaaaggtcag aaagaagctt gacgagtata ttaaggaaat
     4381 tgaaacttgt atagaacttt tagaaaacct gtaagaaatg gcagcggagg aaagtaaaca
     4441 gatatctgtg attattgccg gccgtgggta tccccttaaa atcaaagagg gagaagaaga
     4501 tgccattatg atgatagcag atgaagtcaa caaaaggatc agagaatatc aggaagtata
     4561 tcccaacagg gaaaaacagg actggttagc catggcgata ctatcttatg cgatagaacg
     4621 ctttcaatcc gaaaaggcga tgacagactc aaagttagta caaagatttg acgaagtaga
     4681 acactacctt gacgatcttc tgaaataaac cttcatatac cactttccga cccggttagt
     4741 attaattgtt taaccctggg agtggcagca agtttgcgcc agccccttca aaagaccgag
     4801 tcatggatag cacaatgatt attatctttg gaataattgg cggagcagca ataggatttt
     4861 ttgtcggtaa tctgattctg aaaaaaagaa tgcaaaacca ggttgacgag atgaatgcca
     4921 aggccgattt gcagattcag gaagcccgac ttacggctaa aagaatagtg gatgacgctg
     4981 aagtaaatgc cgaaaaaatc atctctaagg ctgaaagaga gaatgaaaag aaaaaacagc
     5041 aaaagatcgc tgaagcgaag gaaaattttg aaagactgaa atcccagttt gaaggcgaaa
     5101 aagataaatt ctactcaaag ctcaaagaaa gggaaatcga agttgtaaaa aaagagaccg
     5161 acattaaaca aacccaacaa aaccttcagc aacagaaaga aaacttagac tctaaagaaa
     5221 ccgaacttaa gctgatccgg gaaaatcttg acacccaact aaagattgtc aataggagaa
     5281 aagacgaact ggaagcctct cacgaagccc acatcaagca acttgagtcc atatccaaac
     5341 tgagccagca ggaagccaag gagcaactta tacagtccat gacggccaag gcagaaaatg
     5401 aagccatgtc ccttgtcaaa gataccattg aacaagccaa acagactgcc aataaggaag
     5461 cccgaaaaat catcatccaa tccatccagc gtatgggtgc tgaaatcacc attgaaaaca
     5521 cagttacggt cttcaatctg gaatctgacg acattaaggg ccagatcatc ggacgtgaag
     5581 gtcggaatat cagagccatt gaagcagcta ccggcgtaga gatcgtcgta gatgatacgc
     5641 ctgaggcaat catactttcc agttttgatc ctatcagaag ggaaatagca agactttcac
     5701 tcaagaagtt agtgactgac ggccgcatcc atcctgccag aatcgaagaa gttgtggcaa
     5761 aaaccaaaaa acagatagaa gaacaaatca ttgaaattgg agagcgaaca gtaatcgatc
     5821 ttgacattca cggacttgat ccatatctta taaagatggt aggaagaatg agattccgat
     5881 catcatatgg acagaaccta ctgaaacact ctattgaaac aagtaacctt tgcgcaatca
     5941 tggccagtga gctcggtctt aaccccaagc agatcaaaat ggccaaaaga gcaggattgc
     6001 tccatgacat cggtaaggta tcggaggagg aaagtgaatt gtcacacgcc atcctcggca
     6061 tgaagattgc agaaaaacac ggcgaagtaa aagatgtcat caacgcaatc ggagcacacc
     6121 atgatgagat cgagatggac aatattatct ccccgattat tcaggcttgc gatgccatat
     6181 caggtgccag accgggcgcg cgacgagaaa ttctggaaag ttacatgaaa c
//
LOCUS       DAOQQN010000180         6198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5407_length_6198_cov_5.070324, whole genome shotgun sequence.
ACCESSION   DAOQQN010000180 DAOQQN010000000
VERSION     DAOQQN010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5407_length_6198_cov_5.070324"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PLG24_07700"
     CDS             complement(<1..500)
                     /locus_tag="PLG24_07700"
                     /inference="COORDINATES: protein motif:HMM:NF019145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24811.1"
                     /translation="MKKLIIYTILCILPMYSFGQLGKKKPSVNKTNSPAQLNISFEKL
                     RQHQAGTSNTFDIPFYPKSFGTMLASGPGARIKAYSITGLPVWIEGKLMDIPVNNRQD
                     VSTTALEFIHVNQATFSIKNAKEEFRITNTSSDDIGNVHVRMQQEYKGIEVWNSEVMV
                     HTREGDP"
     gene            complement(500..931)
                     /locus_tag="PLG24_07705"
     CDS             complement(500..931)
                     /locus_tag="PLG24_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24812.1"
                     /translation="MKFPVFILTIFIISFIGCKSTQLPPDKYDKSSITFGSGGGFSGA
                     YHEYILLDDGRLYATKLDGSSPDYIKNVENKITGQLFDNIRTFGLDKFNYNEPQNLYY
                     FLKYKSQRDSSNIVWSGMPEGKIAIAYSMYRILIEQTQNKK"
     gene            1078..1509
                     /locus_tag="PLG24_07710"
     CDS             1078..1509
                     /locus_tag="PLG24_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24813.1"
                     /translation="MKGLFFVLAFVGFVGFSNLNAQSCCAKKTASVEKVSDEDIQKAA
                     SLDKTVKVKKEKDGTITYTRKEKDPVTGKKVRVPVVYDAATAKFVNQPAETTGKACAG
                     EMKEGKSCSKKEGAAASGSCCSKKGSASVDKPHPAESSSKM"
     gene            2039..3268
                     /locus_tag="PLG24_07715"
     CDS             2039..3268
                     /locus_tag="PLG24_07715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ24814.1"
                     /translation="MQANSTANNKHIIFLILVASLGYFVDIYDLVIFSIVRIPSFADL
                     GIPEQQMRVGGEFVLNMQMGGLLIGGVIWGIIGDKYGRIKVLFGSILLYSLANIANAF
                     VNDLHSYAIIRFIAGLGLAGELGAGVTLVNETMHKEKRGYGTMLIATIGVLGAVAAFY
                     IAEHYAWRNAYIAGGIMGLLLLLLRLGTFESGMFKKSMDDHVAKGSITLLFNNRERRM
                     RYLYCLLIGLPIWFVVGIFVTQAPEFGKALGAPVTLSAGKGILYTYIGISLGDLLAGL
                     LAQITKSRKFTVFVFQLAIIVSSLIYLGSDGISESKFMWIAFFMDLGVGYWATFVTIA
                     SEQFGTNLRSTVTTTAPNFVRGALIPSTFLFEFFVRHYGILTAGYIMIFLLTAIAIWA
                     LSRLRESFNKDLDYLEV"
     gene            complement(3376..5571)
                     /locus_tag="PLG24_07720"
     CDS             complement(3376..5571)
                     /locus_tag="PLG24_07720"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ24815.1"
                     /translation="MSTSAQTFTFADVAIIKNGKRLVNALDGGMNSPQFSQFDIDGDG
                     NLEIILFDKVGDVFSVYRLNTSTNEYEFWQDPPVVFPLVNDMALIRDYNGDGIMDMFA
                     IPTDDIIGFAVWKGVKKGAVTMLERVQLNKWYFNVLSYPGNNGRLNVYTTSIDIPDIQ
                     DIDGDGDLDIFSFNEEGSHMVFYKNNSVELGFGKDSLLYTLEDYCYGKFLEGGLNNDI
                     TLSPDPNKCARNFDGEDPIELRHSGSTILIKDIDHDGKKDILLGDVSFNNVVYLRNTG
                     SNTKAHVTEEVKNFPEGDEAVDLGPFPSVYALELGQEKSECIVATSNAGLIEAERQLN
                     WLYLLDTTRENNFRLVQKDFIINNSFDMGLENNPAILDVDADGRPDLVIGNKFFKEKD
                     NSQYGQLVYLRNISEGGKLSFEIMDEDFAGLKSFGIKHFGFKPTFTDIDHNGFMDMLI
                     GTEKGTLLHYESKNPIGQPIEFELKSESYQNIKVPSRAAPEALDFDGDGDIDLIIGDK
                     NGNFCLFVNTGSATQPIFTPSFQDAPNVYPFGKVSVKEVGNTEGDACPSIIKLNGKNI
                     LVSGSYSGKIFAYEGLEQGSNTTLSPVSINSGNIYTGRQNNPVFGDLDGDGLLEMIQG
                     NIRGGFTIMRTNITTEGNLSVNNSGVKETIRIFPNPINSGSLLYVKNYKEITQADVFS
                     LTGKLVETYKEIHKNGAISLPIGLEPGIYVLRIRLKNSQVFSEKIIVVD"
     gene            complement(5654..>6198)
                     /locus_tag="PLG24_07725"
     CDS             complement(5654..>6198)
                     /locus_tag="PLG24_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ24816.1"
                     /translation="TGTLLIVDEIQTGCGRTGSWFAFQQMDIIPDILLLAKGFGGGLP
                     LGAFIAGKKIMRVLSEKPILSHITTFGGNPVCCASSLATLKYIEENNLVESVAARSSI
                     IDKVFTGYTIRKSGLLAAIQLNSFDEVHKVCMSCMADGVLIDWFLFNDSSIRLAPPLT
                     ISESELENSLAIVKKNIEKL"
BASE COUNT         1742 a         1196 c         1175 g         2085 t
ORIGIN      
        1 ggatctcctt cgcgtgtatg aaccatgact tccgagttcc aaacttcaat ccctttatat
       61 tcttgttgca tgcgaacatg gacatttcct atatcatctg aagaagtatt agtaattctg
      121 aactcttctt tcgcattctt tatactaaag gttgcctgat tgacgtggat aaattccagg
      181 gctgttgttg atacatcctg tctgttattg acaggtatat ccattaattt tccttctatc
      241 cacaccggta gtccggtgat actgtaagcc ttgattcttg ctcccggtcc tgatgcgagc
      301 atcgttccga aggatttggg ataaaatgga atatcaaaag tgttggaggt gcctgcctga
      361 tgttgtctca gtttctcgaa gctgatatta agttgagcag gactattggt tttattaacc
      421 gatggttttt tcttaccaag ctggccaaat gaatacattg gcagaataca tagaatcgtg
      481 taaatgatta gctttttcat cattttttat tttgagtctg ttcaattaaa attctataca
      541 ttgaataggc gatggctatt ttgccttcag gcattccaga ccagacaata ttactggaat
      601 cgcgctgaga tttatatttc aaaaaataat agagattctg aggttcgttg taattaaatt
      661 tatcaagacc gaatgttctg atgttatcaa agagctgccc ggtaatttta ttttcaacat
      721 tttttatata atcaggacta ctgccatcca attttgtagc atatagtctt ccgtcatcca
      781 gcaggatgta ttcatggtag gcgccagaaa acccgccgcc agatccaaat gtgatggaag
      841 atttgtcgta tttgtccgga ggtaattggg tagacttaca accaatgaat gatataatga
      901 atattgtcaa tataaatacc ggaaatttca tagatttaaa ttaacatttg aagactaaat
      961 aaggaatata attaaaattt agttaaaaac atttcaatta tacttgcttt atgccaaaga
     1021 taacaattta gacattatat ttgcgttaaa ttataaagtt aaaattaata gactattatg
     1081 aaaggattat ttttcgttct cgcatttgtt ggatttgttg gattttcaaa tctaaatgca
     1141 caaagttgct gtgcaaagaa gactgcctcc gttgaaaaag tgtctgatga agacattcaa
     1201 aaagctgcat ctcttgataa aactgtcaag gtaaagaaag aaaaagacgg aacaatcact
     1261 tatacccgga aggagaaaga tccggtgaca ggtaagaagg ttcgtgtacc ggttgtctat
     1321 gatgctgcca ctgctaaatt tgtaaatcaa cctgctgaaa ctaccggaaa ggcttgcgca
     1381 ggtgaaatga aagagggtaa gagctgctct aagaaagaag gtgctgctgc ttctggtagc
     1441 tgctgctcta agaaaggtag tgcgagtgta gataagccac atccggcaga gtcttcttcc
     1501 aaaatgtaat tattgaataa tcagaaatat taagcacggg ctcaacccgt gctttttttt
     1561 gagtttacca atctcaaaag cgttttttaa ggaagatttt ctttgtgtaa atctcagcag
     1621 gattggataa tatacacctt ttcagttttt agcctggtcc cttaattgta ggaaaaggga
     1681 caaactctat attcacctgg ttcttaaatt attttgtgaa acacgcaggt tcatttttcc
     1741 ttggttcaac ctctttgcaa caaccagttt aacttagcta tattttttgc gaatccgtga
     1801 tttggattaa gcaatagatc tctacctgta ctttccttag attgcacaca ctctttcctg
     1861 tattcagatt ttgaagttac cgcgcacctc cagtttctac cttgattgct ccaattaacc
     1921 ataactcata ggtttcaatc atccttattt tgcttatcat tcaacttcgg gatgtatttt
     1981 ttttgattta aattaatctg tttcggtaat tttaattttt aaactgaatt gatgaaccat
     2041 gcaggctaat tcaacagcta acaataagca tattattttc ttgatcctgg tcgcttcttt
     2101 gggttacttt gtggatatct atgaccttgt cattttttct attgtaagga ttccaagttt
     2161 tgccgacctc ggaattcctg aacagcaaat gcgtgtcggt ggagagtttg tgttaaatat
     2221 gcagatgggc ggtttgttaa taggtggtgt catttggggg attatcggtg ataaatacgg
     2281 cagaattaaa gtactgtttg ggtccattct gttgtattct ctggcaaata ttgcaaatgc
     2341 ctttgtcaat gatttgcatt cctatgctat catacgtttc attgccggat tagggcttgc
     2401 cggtgaattg ggggctggtg tcacattggt taatgagacc atgcataaag aaaaaagagg
     2461 ttatggcacg atgttaattg cgaccattgg agttttggga gctgtagctg ctttttatat
     2521 tgctgagcat tatgcatggc gtaatgccta tattgccggt ggaattatgg gcttgttgct
     2581 tttattgctt aggttgggta cttttgaatc cggtatgttt aagaaatcga tggatgatca
     2641 tgttgcaaag ggaagcatca cgttgctttt caataatcgg gaacgaagga tgagatatct
     2701 atattgttta ctaattggcc ttccaatttg gtttgtcgta ggtatttttg ttacccaggc
     2761 acctgagttc ggaaaagccc tcggagctcc ggttacccta agcgcaggta agggtattct
     2821 ttatacttat attggaattt ctttgggaga tttattggca ggattattag cccagattac
     2881 taagtccagg aaattcactg tatttgtctt tcagcttgct attattgtta gcagtttgat
     2941 ttatcttgga tccgatggaa ttagtgagtc taagtttatg tggatagcat tttttatgga
     3001 tcttggtgtg ggctattggg cgacctttgt cacaatagca tccgagcaat tcggtaccaa
     3061 tctccgatct acagtaacga ctactgctcc aaattttgtc cgtggggcct tgatacccag
     3121 caccttttta tttgaatttt ttgtcaggca ttatggtatc cttactgctg gctatatcat
     3181 gatttttctg ctgacagcaa ttgctatatg ggcattaagc aggttacgag aaagcttcaa
     3241 taaggatctt gattatttgg aagtttgagc atgatcaaag gatattgaga gcaacttgtt
     3301 agatacagtg tggactatga atatttgctg atattttact caacgatccg gagtgacatg
     3361 tgtcgacccg gatctttaat ctacaacgat tatcttttct gaaaatactt gactattttt
     3421 caatcttatc cttagaacat atatcccggg ttccagccca attgggagag atattgcccc
     3481 atttttatga atttccttat aggtttcaac taattttccg gtcaggctaa atacatcagc
     3541 ttgagtgatt tctttgtaat ttttaacgta gagcaatgag ccggagttga ttggattcgg
     3601 aaagattctg attgtttctt tcaccccact gttattgact gataggtttc cttctgtggt
     3661 aatattcgtt ctcataatgg taaaaccgcc tctgatgttt ccttgaatca tttcgagaag
     3721 gccatctcca tccagatctc cgaagacggg attattctgc cttcctgtat agatgttacc
     3781 cgaattgata gaaacaggag acaacgttgt attactgcct tgttcaagac cttcgtatgc
     3841 aaaaatttta ccactatagg aaccggacac caaaatattc ttaccattca atttgataat
     3901 tgacggacag gcatcccctt ctgtattccc aacttcctta acactaacct taccgaatgg
     3961 atagacattg ggcgcatcct gaaaagatgg cgtaaatatt ggctgtgtag cacttcccgt
     4021 atttacaaac aaacaaaagt taccattctt atctccaata atcaggtcaa tatcaccgtc
     4081 accatcgaaa tccaacgctt ccggagcggc acgagatggt acttttatgt tttgatatga
     4141 ttctgatttt aattcaaatt cgataggttg gccgatgggg tttttacttt cgtaatgcaa
     4201 caaggtccct ttttccgttc ctataagcat gtccataaat ccattatgat caatatccgt
     4261 aaacgttggt ttgaatccaa aatgtttaat tccgaaactc ttaaggcctg caaagtcttc
     4321 atccatgatt tcaaatgaaa gttttccgcc ttcagaaata tttcttaaat acaccagttg
     4381 cccatattga ctattatctt tttccttgaa gaatttattg cctattacaa gatcaggtct
     4441 tccatcagca tcaacatcta agatagcagg attattttcc aatcccatat cgaatgaatt
     4501 gttgataatg aagtccttct gtaccaaccg gaagttattt tcccgggttg tatccaataa
     4561 atacagccag ttaagttgcc tttcagcttc tatcagacct gcattgctgg ttgctacgat
     4621 acattccgac ttttcctgcc caagctctaa ggcatagacg gatggaaatg gcccgagatc
     4681 caccgcttca tctccttcag gaaaattctt tacttcttct gttacatgtg ctttggtatt
     4741 ggatccggta tttctaaggt atactacatt gttaaaagaa acatcgccaa ggagaatatc
     4801 cttcttacca tcatggtcaa tatccttaat aagaatggtc gatcctgaat gccttaattc
     4861 tatggggtct tctccgtcaa aattcctggc acatttattt ggatccggac ttagtgtgat
     4921 atcgttgttc aatccacctt caagaaactt accataacaa taatcttcca gggtgtaaag
     4981 taaagaatcc tttccaaaac caagctctac actattgttt ttataaaaga ccatatggga
     5041 tccctcttca ttaaaagaaa aaatatcaag atctccgtcg ccatcaatat cctgtatatc
     5101 cggaatatct atggaggtag tgtaaacatt taacctgcca ttattgcccg gatagcttaa
     5161 tacgttaaaa taccatttgt ttaactgaac tctttccaac attgtaacag ctcctttttt
     5221 tacccctttc catacagcaa atcctatgat atcatctgtc ggaatggcaa acatatccat
     5281 tattccatct ccgttataat ctcttatcag ggccatatca ttgaccaggg gaaacactac
     5341 cggcggatcc tgccagaatt catattcatt ggtggaagta tttagtctat atacgctgaa
     5401 tacatcacct accttgtcga ataggatgat ttcaagattt ccgtcaccat ctatatcaaa
     5461 ttgcgaaaat tgaggactgt tcatcccgcc atccaaagca ttcaccaacc gctttccatt
     5521 ctttattatt gcaacatcgg cgaatgtaaa tgtctgggct gatgtactca taattcccat
     5581 gaggcaaaaa atgattataa gctgcctgta aatgttttta gaattgaaat ttgatattag
     5641 ttggttcatg agactatagt ttttctatat ttttcttgac gattgccaag gaattttcca
     5701 gttcactttc tgatattgtt aatgggggag ccagacggat gctactgtca ttaaaaagaa
     5761 accaatctat aagtactcca tcggccatac aggacatgca tactttatgt acttcatcaa
     5821 aggaattgag ctgtatggct gctaaaaggc ctgacttcct tatggtataa cccgtgaata
     5881 ccttatctat gatgcttgac ctggcagcaa cactttccac aaggttattt tcttcaatgt
     5941 attttagtgt tgccaatgat gatgcacaac ataccggatt acctccaaag gttgtgatat
     6001 ggcttaaaat gggtttttct gacaaaacac gcattatttt ttttcccgca atgaaggctc
     6061 ccaaaggtaa tccccctcca aatcccttag caagcagcag gatgtcagga attatatcca
     6121 tctgttgaaa ggcaaaccag cttcctgttc tgccacatcc tgtctggatt tcatcgacaa
     6181 tcaacaaggt gcctgttt
//
LOCUS       DAOQQN010000181         6169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5447_length_6169_cov_4.145077, whole genome shotgun sequence.
ACCESSION   DAOQQN010000181 DAOQQN010000000
VERSION     DAOQQN010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5447_length_6169_cov_4.145077"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PLG24_07730"
     CDS             complement(<1..305)
                     /locus_tag="PLG24_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HMZ24817.1"
                     /translation="MPHLEESFLKEAVDENGLHISPVLDDDIKNYLIDIDGTICDDVP
                     NEEPERMVTVEPYYDALEMCNKWYDEGHIITFFTSRTEEHRVITEEWLKQYGFKYHG"
     gene            646..1605
                     /locus_tag="PLG24_07735"
     CDS             646..1605
                     /locus_tag="PLG24_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ24818.1"
                     /translation="MYSSDASGVDALAQATKDLKKEIGKVIVGQESVVQAVLISLFSN
                     GHSLLVGVPGLAKTLLISTISEVLDLEFKRIQFTPDLMPSDITGTEILDDNRQFKFTK
                     GPLFANIILADEINRTPPKTQSALLEAMQERVVTVAGQRYSLPKPFFVLATQNPIEQE
                     GTYPLPEAQLDRFMFNIPLDYPEYQDEIEVVRSTTSDKVANLRHILNADQIIYFQQLV
                     RKVPISDNVLEYAVNLAVKTRPGTERATDEVNKYISWGAGPRASQYLVLGAKCHAAIR
                     GKYSPDIEDVRAIAEYVLRHRIVRNYKAEAEGINESAIIRGLL"
     gene            1618..2025
                     /locus_tag="PLG24_07740"
     CDS             1618..2025
                     /locus_tag="PLG24_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HMZ24819.1"
                     /translation="MKIAVFRTAHFNAAHRLHNPSWSDERNVQEFGVCNNPHYHGHNY
                     ELEVRLAGEVGPETGYLINLDKLKSIIEADVIQYLDHKNLNLEIDEFRTLNPTVENIC
                     YIIWSRLRKALDDRYELRVRLYETPRNYAEYPA"
     gene            complement(2319..3632)
                     /gene="ahcY"
                     /locus_tag="PLG24_07745"
     CDS             complement(2319..3632)
                     /gene="ahcY"
                     /locus_tag="PLG24_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HMZ24820.1"
                     /translation="MEVTDNKLAYKVKDITLADWGRKEIQLAEAEMPGLMSLREQYGT
                     KKPLKGARIAGCLHMTIQTAVLIETLVELGAEVSWSSCNIFSTQDHAAAAIAAAGIPV
                     FAWKGMNEQEFDWCIEQTLHAFSGGQPLNMILDDGGDLTNMVLDRYPELVPGIRGISE
                     ETTTGVHRLYERVENGTLPLPAININDSVTKSKFDNKYGCKESLVDAIRRATDLMMAG
                     KVAVVAGYGDVGKGSAASLRGAGCRVIVTEIDPICALQAAMDGYAVKTMAHAIPEANI
                     VVTATGNLNIVTAEHFKAMKDKTVVCNIGHFDNEIDMAWLNENYGKTKVEIKPQVDKY
                     TIDGKDVIVLAEGRLVNLGCATGHPSFVMSNSFTNQSLAQMELWANSDKYKNQVYMLP
                     KHLDEMVARLHLAKIGVELETLSKEQADYIGVKVEGPFKPDYYRY"
     gene            4208..5875
                     /gene="glgP"
                     /locus_tag="PLG24_07750"
     CDS             4208..5875
                     /gene="glgP"
                     /locus_tag="PLG24_07750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HMZ24821.1"
                     /translation="MNDKFSNFKVPYSPAKQFSTRVAYFSMEFALDQSLKTFSGGLGF
                     LAGSHMRSAYELKQNMIGIGILWKFGYYDQVRNSDNNMAVLWQQKSYNFLEDTGIRFQ
                     VDVSDHPVWVAVWYLPPERFESVPMFFLTTDVPENDYLSKTITNHLYDYNPEAKIAQS
                     IILGVGGAKLIDIINFMPEVYHLNEAHGLSAAFYLYNKYKSAEEVRKRMVFTTHTPVE
                     AGNEKHNFVDLEKMSFFQSIPVAEIRQITGITGNIFDHSLVALRLSKIANGVSAIHGD
                     VARRMWGGYEGIAPITSITNSQNKKYWADYKLEKAASENNVTALEARKKELKYRLFIN
                     LADQTGKWFDPNVLTIVWARRFAGYKRANLITKDLDRFKQVFCNPQRPIQVIWAGKPY
                     PKDNGAIEMFNELVRLSANYPNIAIMTGYEMRLSKLLKQGSDVWLNNPRIPMEASGTS
                     GMTAAMNASINLSTWDGWIPEFVENGKNGFVVPPVDYQNLTEQQQDDQDLINLLNIIE
                     KDIASLYYNNPKSWYKLAIRAVKEVVPEFESGRMATEYYEKLYKYKR"
BASE COUNT         1865 a         1346 c         1241 g         1717 t
ORIGIN      
        1 ataccatgat atttgaagcc gtattgtttc agccattctt ccgtgatgac acgatgttct
       61 tcagtccggg aagtgaaaaa agtgatgata tgaccttcat cgtaccattt attacacatc
      121 tccagagcat cataatacgg ttcgaccgtc accattcttt ccggttcttc attgggcaca
      181 tcatcacaga ttgtgccatc gatatcaata agatagttct taatgtcatc atcaagaacc
      241 gggctaatgt gaaggccgtt ttcatcaact gcttccttta aaaaggactc ttcaaggtgt
      301 ggcatggata atatttaatt cattgttcca agcagcaaag atagatagaa tgattcaatg
      361 aataatcaat tttaatgaat atttaaggaa gcaattggaa tatttagcaa ataacaatta
      421 taaatccaat atcctgatat gaataatcta ttatgaatgc ccaatttatt aaaggccgct
      481 gttgccttct caggcaaaga aaactgagaa ggcggacaaa aaaccatgaa tcaacaaaag
      541 cagctaaaca attccaactt tgtattgttt tttgattttc cggtacttta caggaaaatt
      601 atgcgttata tttggcgcca ttatttattc atttaaaata tcaagatgta ttcttcagat
      661 gcaagcggtg tggacgcttt ggcacaggcg acgaaagacc tgaaaaaaga aattggaaag
      721 gtcattgtcg gacaggaaag cgtagttcag gcagtattga tttcattatt ctcgaatggg
      781 cacagccttt tagttggtgt acccggactg gccaaaacgt tgttgatcag cactatctca
      841 gaagtactcg atctggaatt caaaagaata cagtttactc cggacctaat gccttccgac
      901 atcacaggta cggaaatcct ggatgataac cggcaattta aattcactaa aggtccgtta
      961 tttgctaata tcatcttagc cgatgaaatc aaccgtacac cgccaaagac gcagtcagcc
     1021 ctgctggaag ctatgcagga aagggttgtg acagttgccg gacagcgata ctcactaccc
     1081 aagccgtttt ttgtattagc tactcagaac ccaatcgagc aggaaggtac ttatcctcta
     1141 ccagaagccc agttagaccg attcatgttc aacattccgt tggattatcc ggaatatcag
     1201 gatgaaattg aggtggtcag gagtaccact tctgataagg tggccaactt aaggcatatc
     1261 ctcaatgccg atcagatcat ttatttccag caacttgtac gcaaagtccc catctccgac
     1321 aatgtcctgg agtatgccgt caatctcgcc gtaaagacca gacctggaac ggagagggcg
     1381 acagatgaag tcaataaata catttcctgg ggtgccgggc cgcgggcatc gcagtatctg
     1441 gttctcggag ccaagtgcca tgcagccatc agaggcaaat attccccgga tatagaagat
     1501 gtaagggcga tcgctgaata tgtcctacgc caccgtatcg tgaggaatta taaagctgaa
     1561 gcagaaggca tcaatgaaag tgccatcatc agagggctcc tttaatccga atcttccatg
     1621 aaaattgcag tatttcgaac agcacatttt aacgccgctc acaggctcca caatccctcc
     1681 tggagtgacg aacgcaatgt ccaagagttc ggagtatgca ataaccctca ttatcacgga
     1741 cacaactacg aacttgaagt cagattggcc ggagaggtcg gtcccgaaac cggttatctg
     1801 atcaaccttg ataagctgaa atccatcatc gaagccgatg tcatccaata tctcgaccat
     1861 aaaaacctca accttgaaat cgatgaattt cgtacattga atcccactgt tgagaacata
     1921 tgttacatca tctggtccag acttcgcaag gcgctcgatg atagatacga actaagggta
     1981 aggttgtatg agacaccgcg gaattatgca gagtatccgg cgtgattaac attagtccgg
     2041 tgtatggcat gattgaattt tgacaaggtc atacctttca aatttcattt attttatatg
     2101 ataaagtgaa aatggcttac tatgaatgat gcattatcgt gttttcatca taagtgaact
     2161 tacatttatc ccctatttct catgtcaagg taatgttcaa aatagtttct ataaaattat
     2221 tcccattgcc aactgacatc catcaaaaaa gccattcccg acagcaggaa tggcatttgt
     2281 ctatttgaat attggcaggt tagaagctgc caaatggatc aatatctgta ataatccggc
     2341 ttaaaagggc cttctacctt aacgccgatg taatcggctt gttctttact cagtgtttca
     2401 agctcgacgc caatcttagc taaatgcagc cttgcaacca tttcatcaag atgcttcggt
     2461 agcatataaa cctggttttt gtacttatcg ctattggccc aaagttccat ctgggcaagg
     2521 ctttgattag taaatgaatt ggacatcaca aaggaagggt ggcctgttgc gcatcccaga
     2581 ttgaccaggc gaccctcagc caaaacaatt acatccttgc catcgatggt atacttatcc
     2641 acctggggct tgatctcaac cttggtctta ccgtaattct cattgagcca tgccatgtcg
     2701 atctcattat cgaagtggcc gatattgcag acaactgttt tgtccttcat agccttgaag
     2761 tgctcggcag taacgatatt caggttaccg gtagcagtca ctacgatgtt ggcttccgga
     2821 atggcatgag ccatagtctt gaccgcataa ccatccatgg cagcttgcag ggcacatatc
     2881 gggtcaattt cagtaacgat gacgcggcag cctgcaccac gtagtgacgc ggcagagccc
     2941 tttcctacat caccataccc tgcgacaaca gcgaccttac cggccatcat caagtcagtt
     3001 gcgcgacgta tagcatccac aagggattct ttacatccat atttgttgtc aaacttggac
     3061 ttcgtgacag aatcattgat attgatagca ggtagtggca gagtaccatt ctcaacccgc
     3121 tcataaagcc tgtgcacacc ggtagtggtt tcctcactga ttccgcgaat gcccggaacc
     3181 aattcaggat atctgtccaa taccatatta gtcaggtcac caccatcatc caggatcatg
     3241 ttcaacggct gaccaccaga aaatgcgtgt aaggtttgct cgatacacca gtcaaattct
     3301 tgctcattca tacctttcca ggcaaagacc ggaataccag cagcggcaat tgctgcagcg
     3361 gcatgatcct gcgtagagaa aatattgcat gaactccagc tgacctcagc acccaactcc
     3421 acgagtgtct cgattagtac agctgtctgt atagtcatat gcagacaacc cgcgatccgc
     3481 gcccctttca agggtttctt ggtaccatac tgctccctga gtgacataag accgggcatt
     3541 tcagcctccg ccagttggat ttcttttctc ccccagtctg ccagggtaat atctttgact
     3601 ttgtaagcca atttgttatc cgtaacttcc attaaatatt ctgatttttt aatattggcc
     3661 gcaaaggtac gactttatag gaaaatattc gaaccaatat atgccaattt tataacaagg
     3721 attaaaccct tgagtattcg cctctcagta caagccggac aaaacattga atcaattaaa
     3781 tcgaatacaa ccggatctca aagcagttta aaaaaaagca tttaaagcca ttatataaac
     3841 aaattaatta tacatatatg cctaattatc tatattttac aatagaactg aaactcaatc
     3901 acagttgaat aactgattgt aatattaagt tcaacacacc aattcctgta cataacctgg
     3961 tagaggtctc agcttattgc tgaacaattc tcctgatttt ccccatcaaa atatgtggct
     4021 aaataatatt acctgattca ttccatgttt ttggttgttc gctttaaatt ttcaagacct
     4081 gctgtatatt cttaatctac cggtttgccg atggtatggc caaatacaat attcaacgtt
     4141 taggatatta tttttagtta tatatttatt tattctattt ttgcccttta ctatatgatt
     4201 ttaatcaatg aacgacaagt tttcaaattt taaggttccg tattctcctg ctaaacaatt
     4261 ttcgaccagg gttgcctatt tctccatgga atttgcactg gaccaatcgc tgaagacgtt
     4321 tagcggcggc ttgggtttcc tggccggatc acatatgcgc agcgcttacg aattaaaaca
     4381 aaacatgatt ggaatcggga ttctgtggaa gttcggttat tatgatcagg tacgtaattc
     4441 ggataacaat atggctgtac tttggcagca gaaatcctat aattttctgg aagataccgg
     4501 cattcgcttc caggtcgatg tcagtgatca tcctgtctgg gttgctgttt ggtatctgcc
     4561 acctgaacgc tttgagagtg tacctatgtt tttcctgacc acagatgtac cggaaaacga
     4621 ttatttatcc aaaacaatca ccaatcacct ctacgattac aaccctgaag ctaagatcgc
     4681 ccaaagtatc atcttaggtg tcggaggtgc caaactgatt gatatcatca atttcatgcc
     4741 tgaggtctat cacttgaatg aagcacatgg actttcagct gctttttatc tatataacaa
     4801 atacaaaagt gctgaagaag ttcgtaagag aatggtattc accacccata caccggttga
     4861 agctggaaat gaaaagcaca actttgttga tttggaaaaa atgagttttt tccagtctat
     4921 tccggtggct gaaatcaggc aaataacagg tataaccggc aatattttcg accattcctt
     4981 agtggcactc cggctttcca agatagccaa tggcgtttca gccatccacg gagatgtagc
     5041 acgaaggatg tggggcggat atgaaggcat cgcgccaatc accagcatca ccaatagcca
     5101 gaacaaaaag tattgggctg attacaaact cgaaaaagca gcttcagaaa acaacgtgac
     5161 ggccctggaa gccagaaaga aagaactgaa atatcgtctc ttcatcaatc tggccgatca
     5221 gacaggcaaa tggtttgacc caaatgtcct gactatcgta tgggcaaggc gttttgcggg
     5281 atataagcgt gccaatctga ttaccaaaga cctggatcga ttcaaacagg ttttttgtaa
     5341 cccccaaagg cctatccagg ttatttgggc gggtaaacca tatccgaagg ataacggggc
     5401 cattgaaatg tttaatgaac tggtccgttt aagtgccaat tatcccaaca tcgccatcat
     5461 gacaggctat gaaatgagat tgtctaaatt gcttaaacaa ggaagtgacg tttggctcaa
     5521 caatccaagg atacctatgg aggcttccgg caccagcggc atgacagcag caatgaatgc
     5581 tagtatcaac ctttcgactt gggacggatg gattccggaa tttgtcgaaa acggcaagaa
     5641 tggttttgtt gttcctccgg tcgattatca gaacctgact gaacaacaac aagacgacca
     5701 ggacttaatt aatctgttga atatcataga aaaggacatc gcttcacttt attataacaa
     5761 cccgaaatcc tggtacaaac tcgctatccg ggcagtgaaa gaagttgttc cggaatttga
     5821 atcaggcagg atggcaacag aatattacga gaaactatat aaatacaagc gttagtaaaa
     5881 tataagttgg aagtacaaag gtctcgaact gtggattatg attcaacggc atttaattaa
     5941 aacaatgaac ccgatcggga tttaatattc ataaaaaaaa atcatgggaa aacaacagta
     6001 cgacaattta agagtcgaat ttattttctc actaaagctc aatgataaat aatattcttt
     6061 tgaggccaaa aaccatatcc aggcttgacc ggtcattacg aatcatggga gaggggcaaa
     6121 tttccaattt ttcatgatta aggattcaat caattattag gttagtaca
//
LOCUS       DAOQQN010000182         6129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5504_length_6129_cov_6.536878, whole genome shotgun sequence.
ACCESSION   DAOQQN010000182 DAOQQN010000000
VERSION     DAOQQN010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5504_length_6129_cov_6.536878"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..671
                     /locus_tag="PLG24_07755"
     CDS             225..671
                     /locus_tag="PLG24_07755"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMZ24822.1"
                     /translation="MKNLMTDKSIFVKSLVLVVCAAIVILLPSAASIKTVNLNGKIKY
                     DQSTVASDAAVSLLNETDLSLVTSVITDREGNFRFAKLKPGKYFVAAGAFGNKLEVFG
                     PYEVSGKDRNITIEELIIKRPSGTTGPIIKAKKDHAPVINRSIRIS"
     gene            complement(950..1570)
                     /locus_tag="PLG24_07760"
     CDS             complement(950..1570)
                     /locus_tag="PLG24_07760"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HMZ24823.1"
                     /translation="MPITTDIPEGYERIKKTIDIYRANIYGFLILIPVSAMYLLPYYL
                     VWQKIPAIGSILSDQGHPPIRVLIYTIVALVAGVVLHELIHGITWSFFTRHGFRSIRF
                     GIMSKALTPYCHCKEPLSKNQYILGAIMPSILLGVIPGIWAIAMGNAVALYWGVFFTI
                     AAVGDFMIISLIRKEARNVFILDHPTEAGCYVFRRKTTPEKDESIL"
     gene            complement(1687..2499)
                     /locus_tag="PLG24_07765"
     CDS             complement(1687..2499)
                     /locus_tag="PLG24_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ24824.1"
                     /translation="MQVKDKTVVITGGGNGMGREVVLNMLHKGARVAAVDVNGSALEE
                     TKRLAGANADRLSIHVLDITDKDAVFAFPQIVIAEHGSVDIVINNAGIIQPFVRVNDL
                     PYDKITRVFDVNFFGLLYMTKSFLPHLLERPEAHLVNVSSMGGFLPVPGQSIYGASKA
                     AVKLLTEGLYAELRHTRVHVSIVFPGAIGTEITRNSGVDVPNMAAGVDAKKQAARTTS
                     PDKAAEIIVNGILKNKNRIFVGGDASFLDRLYRLAPTFATNFIAGKMKDLLK"
     gene            complement(2668..5439)
                     /locus_tag="PLG24_07770"
     CDS             complement(2668..5439)
                     /locus_tag="PLG24_07770"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ24825.1"
                     /translation="MRSALFLLIFVLGALTAHSQNYRILGVVNDQIDSSSLIGASVFL
                     KSLPDSNTVAVATTDAGGRFEFKDLKAGPYVIQSTYVGYKTFSKAVELGFSAAFSIEI
                     MMRGDGITLGGMEVIGRTPPVTVKEDTSEIRANQFKVNPDANAEDLVTKMPGIQKVDG
                     KIQAQGEEVKRVLVDGAVFFGSDPSATLKNLPAEMIDKVQVFDKASDQAQFTGISDGN
                     EEKTINIVTKPDYRQGKFGRVFAGYAPDDKYKAGGVFNSFNKAQRLTIIGQSNNVNEQ
                     NFAADDMAAMQTTSGGGMRGHGGPGGPPRGGGEFGRNNNPFMTNTSGGIVTTHALGVN
                     YTDKWADKVVFQGSVFGNLTNTNLIQSSFTEYFTGQNSKDNTVSDRDGKSFRFNARVE
                     YTIDSFQSVIYTPSYNYGYNTSNYINNSDIFTGETPLSKYDNKSSSYGKNVSFNNELM
                     YRKRFSTQGRTISLRLDQSYSNNLPRTDQNLLTISYDTVNIEILEKQYVESNNYNRSY
                     SAEVNYTEPIAKDHFLMFGYNYSIKDNDIDKRLYDLGNGEQPGEQNLITSLSNVTDNL
                     YQSHRPRLDYRFQNKKLNLSAGVGYQYSILNTDKTFPQVEHVNKTFKNVLPSAMVRLS
                     ISKTKNFRLFYRAGTQEPSVSQLQSVIDNSNTLQLTSGNPTLDQSTTHRMMMFFSSTN
                     QQTGSNFFIGGGGMATKDYIASQVIYSDKDTILAGYGNMPAGARYTYPINLDGYYSIR
                     GFSSFGFPFYLIKSNVSLNVNGSMINAPSKINSQINHSRSKNVGGGVTISSNFSQNLD
                     FTIATQVGFSDVTNDINTQADNTYFNQNVSGRFNWIFFKNFVFSTDAAYYYNKGLESV
                     SQTNYTIWNAAFGYKFLKKNAAEIRIQAFDILNNNTALTRNVTETYYADVSTNVLNRY
                     FMLQFSYKF"
BASE COUNT         1729 a         1276 c         1322 g         1802 t
ORIGIN      
        1 ctttgaccgt tttttaacat aatttgttga ataatgcgca acttgggtta atttggatga
       61 aaaattaatt cgtatatcgt gaataagtac gatttcatcg aaaaccacat accatcgtgt
      121 agtagttcgc tacatgccat acgtttcaac actacaaaag tcaattaata aaagagcaca
      181 attccttttg acatagattc taattaacct taaaaaattc taaaatgaaa aatctaatga
      241 ctgacaaatc catttttgtg aaatcactgg tgttggtagt atgtgctgcc atcgtaattt
      301 tacttccatc cgctgcttca atcaaaacag ttaacctgaa cggaaaaatc aaatatgatc
      361 aatcaacagt tgcctctgat gctgcagtaa gtctgctcaa tgaaactgat ttgtcgttgg
      421 ttacttcagt tattacggac agggaaggta attttcgatt tgcaaaactg aagcctggca
      481 aatactttgt ggcggcaggc gcttttggca acaaactgga ggttttcggt ccctatgaag
      541 tatccggaaa ggaccgcaat ataacaatag aagaactgat tattaagcgt ccatctggaa
      601 ctacagggcc gatcatcaaa gcaaagaagg accacgcacc ggttataaac cgctcgatca
      661 gaatatcata aaccatgcaa aaagcgtaaa gtcttgttat catctgttgg ccggctattc
      721 ccagaaattg aaatccctca atgaacatag agaactgaat taattttttg cctgaatttt
      781 tgttgattgc tgctcaaaag agagtggaaa tcaactccga gaaagggttg gctgctaagc
      841 tgcccttttt tttataacag aagtttcgga gtacctcaaa ctgtttattg attggatatc
      901 ccggagatat tgattcaaag ttttttgaaa acacatgaga ctgaaaagtt cataagatgg
      961 attcatcctt ttccggcgtc gtcttcctcc tgaacacata acatcctgct tcggtgggat
     1021 ggtccaaaat gaagacattc cgggcttctt tcctgatcaa gctgatgatc atgaaatctc
     1081 ccaccgccgc aatggtaaag aaaacacccc aatacaaagc aactgcatta cccatggcaa
     1141 tcgcccaaat gcccggaatc acgccgagta ggatggaagg cattatggct ccgagaatgt
     1201 attgattctt gctgagcggt tctttgcaat gacaatatgg cgtcagcgcc ttagacatga
     1261 tgccgaatcg tatcgaccgg aatccatgcc tggtaaagaa ggaccaggtt attccatgaa
     1321 tcagctcgtg gagaacaaca cctgccacca atgcgacaat cgtgtagatc aatacacgga
     1381 taggaggatg tccctgatct gataaaatac tgcctatagc cggtattttt tgccacacca
     1441 gataatatgg caagaggtac atggcagata ccgggatcag aatcaaaaag ccgtaaatat
     1501 tagccctgta aatgtcgatc gtcttcttta tcctttcata cccttcagga atatctgttg
     1561 ttatcggcat ggtaaaaagt catttgaatc aagttataaa atcaggccga agccttatac
     1621 gcgttagtga tacggcaaat atttcacaaa acaggctgaa ctcaacaata aaaacacgtc
     1681 ccgcctttac ttcagcaaat ccttcatttt accggcaatg aaattggttg caaaggtcgg
     1741 ggccaatcgg tataaccgat ccagaaacga ggcatctcct cctacaaaaa tcctgttttt
     1801 atttttaagg ataccgttga cgatgatttc ggcggcttta tcgggcgaag tcgttctggc
     1861 agcttgtttt ttagcatcga caccggcagc catgtttgga acgtccaccc ctgaattcct
     1921 ggttatttcg gtaccgattg caccgggaaa tacaatactc acatggacac gggtatggcg
     1981 cagctctgca tacaacccct cggtgagtaa tttgactgcc gccttgcttg caccatagat
     2041 actctggccg gggactggaa gaaatccgcc catactggat acattgacca ggtgggcttc
     2101 aggcctttcc aggaggtgag gtagaaacga cttagtcatg tagagcaggc cgaaaaaatt
     2161 gacatcgaag acccgggtta tcttgtcata agggagatca ttgactctga caaagggttg
     2221 gatgatgcct gcattattga taacgatgtc cacgcttcca tgctctgcaa tgacgatttg
     2281 cggaaaggca aaaacagcat ccttgtccgt gatatccagc acatggatgg ataatcggtc
     2341 agcgttggcg ccggcaagcc tttttgtttc ttctaaagcg gatccattta catcgacagc
     2401 agcgactcgt gcccctttat gcaacatgtt aagcactact tcacggccca taccgttacc
     2461 gccaccggtg attacaactg ttttgtcctt tacctgcata agttttttct gacaatataa
     2521 tgaatttttt tgtcataagc aaataatcgt atcatatttt acgagacgga aataaaatag
     2581 aagagaggcc cgtagttacc cgacctctct ttgtattgat tggaacatat agttttaaaa
     2641 tccggaaatg aatgccggga gttccagtta gaatttatac gagaattgta gcatgaaata
     2701 cctgttcaaa acgttggtgc tgacatcggc gtaataggtt tcagtcacgt ttcgggtcag
     2761 ggcagtattg ttatttaaga tatcaaatgc ctgaattctg atttcggctg cattcttttt
     2821 gaggaattta tagccgaatg ctgcattcca gatggtgtag ttggtctggc tgacactttc
     2881 cagtccctta ttgtaataat atgcggcatc cgtactgaac acaaaattct tgaagaagat
     2941 ccagttaaac cgacctgaca cattctgatt gaaataagta ttgtcggctt gcgtgttgat
     3001 atcgttggta acatcgctaa atcctacctg tgtggctatg gtgaagtcca gattctggct
     3061 gaaattggaa gagattgtca cgccgccacc gacattcttg gaccgtgaat gattgatctg
     3121 gctgttgatc ttgcttggag cgttgatcat cgagccattg acattaaggc ttacattgga
     3181 tttgatcagg taaaacggaa atccgaagga tgaaaatcca cggatactgt aatacccgtc
     3241 gagattgatc gggtaagtat atcttgcacc ggccggcatg ttgccataac ccgccagtat
     3301 tgtatcctta tcggagtata tcacctggct ggcgatataa tcctttgtgg ccataccacc
     3361 accacctata aaaaagttag agccggtctg ttgattggtg gaggagaaga acatcatcat
     3421 tctatgcgtt gtactttgat caagggtagg atttcctgat gtcaactgga gggtgttgct
     3481 attatcaatt acgctctgca actggcttac cgaaggctct tgagtgccgg cacgatagaa
     3541 cagtcggaaa ttcttagtct tgcttatgct gaggcggacc atggcagaag gcaatacatt
     3601 tttaaatgtt ttattgacat gttccacttg tgggaaggtc ttgtccgtat tcaggataga
     3661 atattgataa cccacaccgg ctgaaagatt gagtttttta ttttgaaagc gataatcaag
     3721 tctgggtcga tgactttgat aaagattgtc ggtaacattg gacagggaag tgatcagatt
     3781 ctgctctccg ggttgctcac cattgcccag gtcataaagt cgtttgtcga tatcattatc
     3841 tttaattgaa tagttgtaac cgaacattaa gaaatgatcc ttggcgattg gctctgtata
     3901 gttgacctct gcactatacg atcggttata attgttgctt tccacatatt gtttttccag
     3961 gatttcaata ttgacagtat cataagatat agtcaacagg ttttgatccg ttcgtggaag
     4021 attattggaa taagattggt ccagccttaa agaaatggtt cttccttgtg tactaaaccg
     4081 ttttctatac atcaactcat tattgaaaga tacatttttg ccataggatg aagacttgtt
     4141 gtcatacttg ctcaggggtg tttcacccgt aaagatgtca gaattgttga tgtagttgct
     4201 tgtgttgtat ccatagttat acgaaggtgt atagataacg gattggaaag agtcgatggt
     4261 gtactcaacg cgtgcgttga agcggaagga tttaccatcg cgatcggaca cggtattgtc
     4321 ctttgagttc tgacccgtaa aatattccgt aaatgatgat tgtatcaggt tagtattcgt
     4381 aaggttgcca aacacacttc cctgaaacac gactttgtca gcccatttgt cggtatagtt
     4441 gacccccaga gcatgtgtgg taacgattcc gccggaggta ttggtcataa atggattatt
     4501 gttacggccg aattcgccac caccccttgg agggccaccc ggtcctccgt gtcctctcat
     4561 cccgcctccg cttgtagttt gcattgctgc catatcgtcc gctgcaaaat tttgttcatt
     4621 cacattattg ctttgcccga taatggtcag acgttgtgcc ttgttgaagc tgttgaatac
     4681 tccacctgcc ttgtatttgt catcaggagc atatcctgca aacactctgc cgaacttgcc
     4741 ctgacgataa tccggcttgg tcacgatatt gatggttttt tcctcatttc catcagagat
     4801 acccgtaaat tgggcttggt cacttgcctt gtcaaagacc tgcactttgt cgatcatctc
     4861 agcgggaagg tttttcaacg tagccgatgg gtcggatcca aaaaaaacgg caccgtctac
     4921 caggacacgt tttacctctt cgccttgtgc ctgaatcttt ccatccacct tttgaatacc
     4981 cggcatcttg gttacgaggt cttcagcatt agcatcggga ttgaccttaa attgattggc
     5041 acgaatctct gaggtatctt ccttgacagt gacaggaggt gtacggccta tgacttccat
     5101 gccacccagg gtaatgccgt ctcccctcat catgatctca atactgaaag cagcgctgaa
     5161 cccgagttcc accgctttgc taaatgtttt atacccgaca taggtcgatt gtattacata
     5221 aggtccggct ttcaaatcct tgaactcaaa ccgccccccg gcatccgtgg tggctacggc
     5281 tacagtattt gaatcaggca aagacttaag gaagacactt gctccgataa gagaggaaga
     5341 atcaatctgg tcattaacaa cgcctaaaat gcgataattt tgagaatggg cggtcagtgc
     5401 tccaagcaca aaaataagta ggaataatgc agatctcaag atttgttacg ttttattttg
     5461 ctgcaaaaaa agtgaataat aatgcgattc tgtgtttcca aactgttaag aaatcccttg
     5521 aaaccagtat ttagacagag gtatcaaatt ttcggattga ataccgggtt tatccgacac
     5581 atgcacaatg attttctatc ggtgttttgt gtcatgcaat aaataaacaa cggccttgtc
     5641 aatcaacaag tcatattaaa tgtttgatgc agcaatttgt ttaaggttta acgtaataaa
     5701 aaaatttttt cgatttttga aacaaatggt gattgtatat cgaaatggaa gatggaggga
     5761 tatcctgagt ctcccatgaa aggaacctgt atttctgcta aatagtatga ataataccga
     5821 aggtgcattt gttatagacc aatttcattg atcgatacga cggtagcctc ggcatcccgg
     5881 aaccccggat tgttttgctc ccgggacaga gcagagctct agtaacatag ctgtccggaa
     5941 aggtatacca agaatttatg gactattata taaaatcatc ttggtataac atttggaatg
     6001 atatgagcca attggtcacg atatggttat aatagatctg tttttttaca ggggaataca
     6061 gctattgaat gatatctccg aaatattccc tggaacagat tgattcaaat ttatgatttg
     6121 taagctttc
//
LOCUS       DAOQQN010000183         6117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5526_length_6117_cov_5.218080, whole genome shotgun sequence.
ACCESSION   DAOQQN010000183 DAOQQN010000000
VERSION     DAOQQN010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5526_length_6117_cov_5.218080"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PLG24_07775"
     CDS             <1..619
                     /locus_tag="PLG24_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HMZ24826.1"
                     /translation="ENSTNKGGGNFYTNAEIEPISVLCHRKGLALHLDGARIFNVLVE
                     TGDSPAAVGALFDSISVCLSKGLGAPVGSVLMGNRDYIREARRIRKVLGGGMRQAGYL
                     AAAGIYALDNNIPQLKIDNQRAAEIGRFLSQQAYVQEVLPVRTNIVIFELAENLSADT
                     FLQTLDSIGIKASPFSATSIRFVFHRDITQEQYDAIIPSLSRITA"
     gene            complement(609..1265)
                     /locus_tag="PLG24_07780"
     CDS             complement(609..1265)
                     /locus_tag="PLG24_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ24827.1"
                     /translation="MIEAEHIHKSFGNVHVLNDVSFSVGKGEVVAIVGKSGAGKSTLL
                     HITGTLEQPDEGNILFEGRNLNKLSSNKLSEFRNNSLGFIFQFHHLLPEFTALENVCI
                     PAYIKKTDRNIAETRAMELLDHLNLSHRLHHKPKELSGGEQQRVAFARALINQPKLIL
                     ADEPTGNLDEKSANELHDLILKFRTEYELTFILVTHSPLLAARCDRVIEMSEGSIVMQ
                     "
     gene            complement(1280..1759)
                     /locus_tag="PLG24_07785"
     CDS             complement(1280..1759)
                     /locus_tag="PLG24_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24828.1"
                     /translation="MRRKFILLTISLLIVQAGWGQKLKTKDVPPRIITTLDKQFKGNK
                     RASWEKADSLYIVNFEYDDQKGMAIFHEKGRMISSEVSVNNEELPFSVNHYLRKNYPD
                     DVSDSIIKARDATKAITYRIIIAGDRLEFDNEGRFLSKEKVQDDLNEINMESNHSEK"
     gene            complement(1854..4817)
                     /locus_tag="PLG24_07790"
     CDS             complement(1854..4817)
                     /locus_tag="PLG24_07790"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HMZ24829.1"
                     /translation="MKNIYLLCAMLASTVMMGQTKEYMAFQRTSPDHKYKFSEVFGDP
                     AKARFYKLDNGLTVILAENHLEPQIMALIATKAGSKNDPADHTGLAHYLEHMLFKGTD
                     KFGTKDYAKEKVELDRIDELYELYGKTTDEAERKAIYHRIDSVSNVASKYAVANEYDK
                     MMSDIGSRMTNAFTSFENTTYMESFPSNNLEKYLKVQQERFRNPVLRLFHTELEAVYE
                     EKNISLDNGDEKIFEAMFGSLFKKHPYGTQTTIGTIEHLKNPSLKEIRNYYNKYYVAG
                     NMAIILAGDLDPDATIAIVEKYFGNMPAKNAPGFAFQAEDDVDTTTKITVYSPDEEKV
                     AIGYRLPGALEKEAPIANLVSSILYNGKSGLIDKDLVIDQKILEGYAFTYLLKDHGIF
                     WMQGKPKDGQSLDQVKELFLEEINKLKRGEFDTTIIQGTVNNMKLDLIRGTEKATNTA
                     FLLHESFVTGRPWVDYLNDMNVMSKITKADIMRFAAKWFQNNYTVIYKKTGNSDVPKV
                     VKPEIHPVELNRDAQSDFLKSIVEAPVSEIQPRFLDFDKDVQKSTLKKGIPLWYVPNQ
                     QNQLFSVYYKYDYGTNANPKLDLAMNYLNYIGSTTRSNSQINKELYNLGLNWGVSVGQ
                     EEMNIYLTGLEENFDKGIEIIEDLLNNPKPDKAALAKLINNQIKARQDNLLNKETILY
                     SGLQNYLKYGAKNPFNSTLTNAQLRQITAEELVAIIKANAGLEHRIMYYGARPLNDLT
                     KSMAARHKTTSMLKPAPKGMEFTPAPSPENTVYFVDYDMVQADINYKANEEKYDPKLE
                     TMARAFNEYYGGGMASIVFQDIRESRALAYSAYSRFGVPNKKNKPFDATFYVGTQADK
                     FNDAMEAINNLLHNMPELTKSWDLCKKAILTEIQTQRINKEGILFAYETALNKGLNYD
                     IRKDIYKNIGSVTMAELKAFHENHFKNQKWNIGVMGSKDKIKVEELKKYGNVKVLTLK
                     DIFGYDEKDFPLIKP"
     gene            complement(4877..5749)
                     /locus_tag="PLG24_07795"
     CDS             complement(4877..5749)
                     /locus_tag="PLG24_07795"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HMZ24830.1"
                     /translation="MDFGRLDDISEVDFSLPEIDHFYFRNNGNNHLELLLGAPGWSEP
                     KWKGSIYPAKAKPSEFAAYYSRQFATIEFNTTYYQVPKPALMERWYDLTEPDFVFCPK
                     MFAGISQSNSFALGDSILPDFLLRMGMLKEKLGLIYMQLPQSFSFVKLESLKKFIRQW
                     RPGIPLAIEIRHESYFKSQNAPVLAEEMAMANISWMITDVAGRRDASHSYITAPFMAI
                     RFVANNDESDFTRIDNWVDRLVVWQTNGVELVYFFLHTKGQPPIVLSEYLAKKWKEKS
                     THDIKKPTVIDREK"
     gene            complement(5751..>6117)
                     /locus_tag="PLG24_07800"
     CDS             complement(5751..>6117)
                     /locus_tag="PLG24_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24831.1"
                     /translation="YRLDIGQLPAGNYRYMARVLFQGKELTSGGDFSVKNNDKELFDL
                     MADFGLLRQISEDSGGRFISVNELDKLADMIKSNDAIKPIIRSDKRTDPLINLKWIFG
                     LLAFLLSAEWALRRYLGRY"
BASE COUNT         1518 a         1298 c         1322 g         1979 t
ORIGIN      
        1 ggagaattct acgaataaag gaggcggtaa tttttatacc aatgcggaga ttgagccaat
       61 cagtgtactg tgtcaccgta agggcttggc gttgcatttg gatggagcca ggatattcaa
      121 tgtgttggtg gagaccgggg actcacctgc ggctgtcggt gccttgtttg attctatttc
      181 ggtttgtttg tccaaaggtc ttggtgcacc tgtaggttcc gtactcatgg gtaacaggga
      241 ttatatcagg gaggcaagac gtatccggaa agttttagga ggaggtatgc gccaggccgg
      301 ttatctggct gctgccggca tttatgcact tgataataat attcctcagc tcaaaattga
      361 taatcaaaga gcagcagaga taggccgttt tttgagtcaa caggcctatg tacaggaagt
      421 acttccggtc cgtacgaata ttgttatatt tgaacttgct gaaaacctgt cagccgatac
      481 attcctccaa acattggatt caataggtat taaggcttcg cccttcagtg caacaagtat
      541 cagattcgtt tttcacagag atatcacaca ggagcaatac gatgctatca ttccttccct
      601 tagccggatt actgcatgac gatgcttccc tcggacattt cgatgacgcg atcgcacctt
      661 gcagcgagca aagggctgtg agttaccagg atgaaagtta attcatattc tgtcctgaat
      721 ttgaggatga ggtcatgaag ctcattggcc gatttttcat ccagattgcc ggttggttca
      781 tcagccagga taagtttggg ttgattgata agcgctcttg caaaggcaac acgttgctgc
      841 tccccacctg ataattcctt gggtttgtga tgaagccggt gagaaaggtt taggtggtcc
      901 agtaattcca ttgcccgggt ttccgcaata ttccgatccg tctttttgat gtatgccgga
      961 atacagacat tttccagagc agtaaattca ggtagcagat gatggaactg gaagataaac
     1021 cccagcgaat tgtttctgaa ttctgataat ttattactgg ataatttatt caggttgcgg
     1081 ccttcaaaga gtatattccc ctcgtcaggt tgttctaagg tacctgtgat gtgtaacaag
     1141 gtactcttgc ccgcaccact tttaccgaca atggctacaa cctcgccttt tccaacacta
     1201 aaggagacat cattcaatac atggacattg ccaaatgact tatggatatg ctcagcttct
     1261 atcatgacag gacggtgttt tatttttctg aatgattgga ttccatatta atttcattca
     1321 aatcatcctg tactttctct ttggacagga atctgccttc attgtcgaat tccaacctgt
     1381 ctcctgcgat gataattcgg taagttattg ccttggtggc atctcttgct ttgatgatgc
     1441 tatcagatac atcatccgga tagttttttc ttaaataatg attgacactg aaggggagtt
     1501 cttcgttatt taccgatacc tcagatgata tcattcttcc tttttcgtga aatatcgcca
     1561 ttcctttttg atcatcatat tcaaaattga caatatagag ggaatctgcc ttttcccaac
     1621 tggctctttt atttcccttg aattgcttat ccaatgtagt aatgatcctg ggaggaacat
     1681 ccttggtttt gagcttctga ccccagcccg cctgaacgat taataagctg attgtgagta
     1741 ggataaattt acgcctcatt tgtttgattt ttatttgtat attacaatat gccccggtga
     1801 ttatagaaat gagcagggac gaatgaaaat acctccctgc ccattttcta ttgttatggt
     1861 ttgattaagg ggaaatcctt ttcatcataa ccaaagatat ccttcaaggt caaaactttt
     1921 acattgccat acttcttgag ttcttcaacc ttaatcttgt ctttgctgcc catgacaccg
     1981 atattccact tttgattttt gaagtgattt tcgtggaatg ctttaagttc agccatagtc
     2041 actgaaccga tatttttata aatatctttt ctgatatcgt agtttaatcc tttgttcaaa
     2101 gctgtctcat aggcaaacaa gattccctcc ttattgattc tttgagtctg aatctcggta
     2161 aggatggctt ttttgcacaa atcccatgac tttgtaagtt caggcatatt atggaggaga
     2221 ttgttgattg cctccatggc atcattgaac ttatcggcct gtgtccctac atagaatgtc
     2281 gcatcaaatg gtttattctt cttattaggt acaccaaagc gactgtatgc agagtaggcc
     2341 agagcccttg attcgcgaat atcctgaaat acgatggatg ccatgccgcc tccataatat
     2401 tcgttgaaag ctcgtgccat ggtctccagt tttggatcgt atttttcttc atttgccttg
     2461 tagttgatgt cggcctgaac catgtcataa tccacaaaat aaacagtatt ttcgggcgat
     2521 ggtgccggag taaattccat gccttttggt gcaggcttaa gcatcgatgt ggttttatgc
     2581 cgtgctgcca ttgatttcgt caaatcgttg agtggtcggg caccataata catgattcta
     2641 tgttctaagc cggcattggc tttgataatg gcaaccagtt cttcggcagt gatctgacgt
     2701 aattgcgcat tggtcaaggt gctgttgaaa gggtttttgg ctccatattt cagataattt
     2761 tggagtccgc tatagaggat ggtttccttg ttgagcagat tatcctgcct tgccttgatt
     2821 tgattattga tcaatttggc caatgcagcc ttgtccggct ttggattatt caaaaggtcc
     2881 tcgataattt ctatgccctt gtcaaaattt tcttcaagac cggtcaggta gatattcatt
     2941 tcttcctggc caacactgac tccccagttc aaccccagat tgtacaattc cttgttgatt
     3001 tgtgaattgg atcgggtggt cgagccgata tagttcagat agttcatggc cagatccagc
     3061 tttggattgg cattggtgcc gtaatcgtat ttgtaataga cactgaataa ttgattctgt
     3121 tgattaggga cataccatag cggtattccc tttttcaagg tagatttctg tacatcctta
     3181 tcaaaatcca ggaagcgggg ctggatctca gatacgggtg cttctacaat ggactttaaa
     3241 aagtcggatt gtgcatcccg attcagttcg actggatgga tttccggctt cactaccttt
     3301 ggtacatcgc tgttgcctgt ctttttgtag ataacagtat agttgttttg gaaccatttg
     3361 gctgcaaatc tcatgatatc agcctttgtg attttggaca tcacattcat atcgttgagg
     3421 taatcgaccc aggggcggcc cgtgacgaag gactcatgca gcaggaaggc agtattagtt
     3481 gccttctcag tgcctcggat tagatccagt ttcatgttgt tgacagtacc ttgtatgatg
     3541 gtggtgtcaa attcacctct tttcaatttg ttgatttcct ccaggaaaag ttcttttacc
     3601 tgatcaagac tttggccatc tttcggtttg ccctgcatcc agaatattcc atgatccttc
     3661 aacagatagg taaacgcata cccttccaga attttttggt cgatgaccag atccttatcg
     3721 attaggccac ttttaccatt atataggata gaggaaacta gatttgcgat gggggcttct
     3781 ttctccaatg ctccggggag acggtagccg attgcaactt tttcttcatc cggactatag
     3841 actgtaattt ttgttgtagt atccacatcg tcttcggcct gaaatgcaaa accgggggcg
     3901 tttttggctg gcatattgcc aaaatacttt tccactatgg caatggtggc atcggggtcc
     3961 agatcgcctg caaggatgat ggccatgttg ccggcgacgt aatatttgtt gtaataattc
     4021 ctgatttctt tgagcgaagg atttttcaga tgttcgatgg taccgatggt tgtctgagtg
     4081 ccgtatggat gcttcttaaa caatgatcca aacatggctt cgaagatctt ttcatctcca
     4141 ttgtccagtg aaatattctt ttcttcatag actgcctcca actcagtatg gaacaatctc
     4201 aaaaccggat tgcggaacct ttcctgttgt actttcagat atttttccag attattggat
     4261 ggaaagctct ccatataggt tgtattctca aaactcgtga aagcattggt catccgcgaa
     4321 cctatatccg acatcatttt gtcatattca ttggctactg catattttga agctacattg
     4381 cttacgctgt ctatgcggtg atagatagct ttccgctctg cttcatccgt tgttttccca
     4441 tacaattcat acaattcgtc gatcctgtcg agttccactt tttcctttgc ataatccttg
     4501 gtaccgaact tgtctgttcc cttaaacagc atatgttcca ggtagtgtgc caggcctgta
     4561 tggtctgcag ggtcattctt tgaacctgct ttagtggcga taagcgccat aatttgtggc
     4621 tcgagatgat tttcggccag aatgacggtg aggccgttgt ccaatttgta aaatctggct
     4681 ttggccggat caccgaatac ttctgaaaat ttgtacttgt gatccggaga agtccgttgg
     4741 aaagccatat attctttggt ctggcccatc attacagtgg aggccagcat ggcacatagc
     4801 aaataaatat ttttcatata tgaatttttt aaatgacaaa gctaatgttt aagtcgaaaa
     4861 tttcgggaat ttatgattat ttttccctat caataacagt cggtttttta atatcatgtg
     4921 tgcttttctc cttccatttt tttgccaggt actcagatag aacgattggc ggttgtccct
     4981 tggtatgcag gaaaaaatac actaactcca caccatttgt ttgccaaacc accaatctgt
     5041 ctacccagtt gtctattctt gtgaaatcac tttcatcatt attggcgaca aatctgatag
     5101 ccatgaatgg cgctgtgatg taactatggc ttgcatccct tctccctgca acatcggtaa
     5161 tcatccagct tatgttggcc atggccattt cttccgccag gacaggagcg ttctgtgact
     5221 taaaataact ttcgtgacgg atttctatgg ctaatggtat gccgggccgc cattgacgga
     5281 tgaatttttt caatgattcc agcttgacga aggaaaaact ttgtggtaac tgcatataga
     5341 tgagtccgag cttttccttc aacatcccca tccgtaacag aaagtcagga agaatggagt
     5401 cgccgagtgc aaacgaattg ctttggctga taccggcaaa catcttcgga caaaacacga
     5461 agtcaggctc cgtgaggtcg taccaacgct ccattaacgc tggcttaggc acctgatagt
     5521 atgttgtatt gaattctatt gtggcaaatt ggcggctgta ataagcagcg aattcactgg
     5581 gctttgcctt ggcaggatat atggatcctt tccatttggg ttcggaccag cccggtgcgc
     5641 cgagtaataa ttcgagatga ttgttgccat tatttctgaa atagaaatgg tcaatttccg
     5701 gcaatgaaaa gtcaacttcg gaaatatcgt ctaatctgcc gaaatccata tcagtatctg
     5761 cccagatatc ttctcagtgc ccactcggct gataaaagaa aggccagcag gccaaatatc
     5821 cacttcaggt tgatgagagg atcggtcctt ttatcacttc ggataatcgg tttgatagca
     5881 tcattggatt tgatcatatc agcgagctta tccaattcat taacactgat gaagcggcca
     5941 cccgaatctt cacttatctg cctcagaaga ccgaaatctg ccatcaggtc aaagagttcc
     6001 ttgtcgttat ttttgaccga aaagtctcct cctgaggtga gttccttccc ttggaacaag
     6061 acgcgtgcca tatagcggta attgccggcc ggaagctggc caatgtccag tctgtat
//
LOCUS       DAOQQN010000184         6073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5588_length_6073_cov_4.959953, whole genome shotgun sequence.
ACCESSION   DAOQQN010000184 DAOQQN010000000
VERSION     DAOQQN010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5588_length_6073_cov_4.959953"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PLG24_07805"
     CDS             complement(<1..226)
                     /locus_tag="PLG24_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HMZ24832.1"
                     /translation="MVDYKKAVAKKLLQINAINLNYNQPFTWASGIISPIYCDNRKSL
                     SDIDARNLICERFVAESERFGSFDVVAGVAT"
     gene            247..870
                     /locus_tag="PLG24_07810"
     CDS             247..870
                     /locus_tag="PLG24_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HMZ24833.1"
                     /translation="MYKIYINEMPVTLIKSENLGQYARVGENILTLPYLGKPKSIHQV
                     IRLSESSNNYKEIILYANDPMVILKAFQSEIKEIVAAGGIVFNEFNEVLFIYRRGYWD
                     LPKGKLDPGETLEEAAVREVEEETGIKNIQLLQYIDDTYHLFKTQKNGNYFLKKTYWY
                     KMQANKQPIKVQKEEDIDEATWLTISTFLKECRPIFNNIVDILGKVK"
     gene            903..1673
                     /gene="kdsB"
                     /locus_tag="PLG24_07815"
     CDS             903..1673
                     /gene="kdsB"
                     /locus_tag="PLG24_07815"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HMZ24834.1"
                     /translation="MIQESNENKIVALIPARLESSRFPGKLLKKLGHKSVILHVHDNM
                     VATGLFDHVAVVCNHRDIYDDIIRGGGSAYMSKKEHENGTSRISEIALELDYPIVFNV
                     QGDEPFVNESILQKLINIFKADKDKNIDIVSPMTILSNEQEIQNPNNVKVVTDNDRNA
                     MYFSRSPIPYHRNQSAPRNCFKHIGIYGYRKEALEKIQKLPYSNLEDQELIECLRYLD
                     YGMKIKMAETPSQGVSIDTPEDFEKAIEFYKILQSGIK"
     gene            1971..2525
                     /gene="ruvC"
                     /locus_tag="PLG24_07820"
     CDS             1971..2525
                     /gene="ruvC"
                     /locus_tag="PLG24_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HMZ24835.1"
                     /translation="MKLSESIIMGIDPGTIKMGFAIIKNAKPIPILLDYGTLNFRHLS
                     DAHDKLHQIFIKIEQLTNKYAVNELAIEAPFYGKNVQSMLKLGRAQGVVISAAKYFGL
                     DTTEYSPRKIKQSVAGNGNASKEQVARMIEKQFGVDLGEAGYDATDALSVAICHAFSK
                     RNILIKNGSKNWDDFIKNNPDRII"
     gene            complement(2534..2929)
                     /locus_tag="PLG24_07825"
     CDS             complement(2534..2929)
                     /locus_tag="PLG24_07825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HMZ24836.1"
                     /translation="MASIFSRIISGELPSYKVYEDDYFFAFLDIRPMVMGHTLVIPKK
                     EVDYIFDLESEEYIGLMLVAQKVAKALKKVVPCTRIGVAVVGLEVPHTHVHLMPINTV
                     SDMNFKNPVVQLTPEKFKSLAEDISTAIE"
     gene            complement(2935..3408)
                     /gene="greA"
                     /locus_tag="PLG24_07830"
     CDS             complement(2935..3408)
                     /gene="greA"
                     /locus_tag="PLG24_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HMZ24837.1"
                     /translation="MANYTYLTQDGLDKLNAELDELKTTGRQEAARAIAEARDKGDLS
                     ENAEYDAAKNAQGMLELRINELEKVLANARLIDASQLDPTIVTLLSTVEIENLKTGKI
                     FTYTLVSEAEADLKAGKISGTSPIGQGLLGKKEGETAIITTPAGKLEFKIIKLSR"
     gene            complement(3514..4575)
                     /locus_tag="PLG24_07835"
     CDS             complement(3514..4575)
                     /locus_tag="PLG24_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HMZ24838.1"
                     /translation="MKRLFTIICLIAFSQIGFAGNPDRQGSAGAGELLINPWARSAGL
                     HTMNTAGISGIEATRLNIAGLASVKSMEIGYSHGRYLVGTGISTNALGLAKKIGKKGV
                     FGLDFMSVNFGDIPVTTVNFPEGNGANFNVAWTNIGLSYAHNFFNEESLGKVSVGLGV
                     RLVMESIPGISATGVAFDAGVQYSNGSKDNFKLGLALRNLGTPMRFRGEGFTVTRPNP
                     NVQNVNLAYDQRSANYELPVVLNIGTSYDVYFADDAVRLTPMINFTSNSFSRDQLGGA
                     VEVSAFKDLVQVRVAYKAEIQKTTEVIKDDVYSGLAFGATANLTINKETKSKVALDYA
                     YMASKVWSGTHNIGVRINL"
     gene            complement(4648..>6073)
                     /locus_tag="PLG24_07840"
     CDS             complement(4648..>6073)
                     /locus_tag="PLG24_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24839.1"
                     /translation="LQYSDATKDNWLSMLRDGGEGVTDNPNISLILSEALNWINYVEK
                     FSGNPDPKGVYEKLGFWPLTLALKEPQDVAVNEFLQPVPPSSNIASTVSKSNTLADLN
                     NVDIVFTNDRSKWSKCIVVEASNTKYSGEGLPPVGSAPQFSLRKSRSITKDFDADGNP
                     VLNANPDSTGMSWFPGYAIDVETGMRLNIFFAENSSYDPAIFGDLIQEVGDNPNCGQD
                     MIFNPGGNLFINASNNLLSAANFFAGGQHYIYVTKTKYNECATFLNNLKGQNPSDINK
                     SRVLAQVTWVGIPLGFNMLSFKDGLIPQETVYKLRVNNSYKVPALADGRTGVNNGMPM
                     YKIEIKDKAADTKSPTIAPSALDKISVVPNPYYAYSSYENTEISNIVKITNLPPKCVV
                     TIYSLDGKFIRQFNRNEVRREVSGEARGVRYAQVTPDIEWDLKNGSGITVGSGIYLIH
                     IKSDDGERVIKWVGAIRQLEISGF"
BASE COUNT         1965 a         1243 c         1083 g         1782 t
ORIGIN      
        1 cagtagccac tcctgccaca acatcgaaag acccaaatcg ttctgattct gcaacaaatc
       61 tttcgcaaat taaatttcgt gcatctatat cgctaaggct tttacgattg tcgcaatata
      121 tcggagaaat gatacctgac gcccaggtaa atggttggtt ataattaaga tttattgcgt
      181 taatttgcag aagctttttg gctacagctt ttttatagtc aaccatatta tctgttttat
      241 cctcaaatgt ataaaatata tatcaacgaa atgcccgtca ctttgataaa aagtgagaat
      301 ctgggtcaat atgcccgggt aggtgaaaac atacttaccc tcccctactt aggaaagcca
      361 aaatcaatac atcaagtaat tcgtctgagt gaatcttcca ataactataa agaaattatc
      421 ctttatgcca acgatccaat ggtaatttta aaggcatttc aatctgaaat caaggagata
      481 gttgccgccg gtggcattgt tttcaacgag ttcaacgaag tgctttttat ctatcgacgt
      541 ggttattggg atcttcccaa aggcaagtta gatccgggtg aaactctgga agaagctgct
      601 gtgcgtgagg tagaagaaga aacaggtata aaaaatattc aacttctaca atatattgat
      661 gacacctatc atctattcaa gacccagaaa aatggaaatt attttcttaa aaaaacctac
      721 tggtacaaaa tgcaagccaa taaacagccg atcaaggtac agaaggaaga agacattgac
      781 gaagccacat ggttgacaat ttcaaccttt cttaaagaat gtaggccaat attcaacaat
      841 atagttgata ttttaggcaa ggtgaaatga agtagcaaca gtattcccac actctccatg
      901 caatgataca agaaagcaat gaaaataaaa tagtagcttt gattcctgcc aggctagaat
      961 caagcaggtt ccccggaaaa cttttgaaaa aattggggca taaatctgtc atacttcatg
     1021 ttcatgacaa tatggttgca accggacttt tcgatcatgt agccgtagtc tgcaatcatc
     1081 gggacattta cgatgacatc atccggggag gaggcagtgc ttatatgagt aaaaaggaac
     1141 atgaaaatgg aacttcacgt atttccgaaa ttgcgcttga attggattat cctattgtat
     1201 tcaatgtgca gggcgatgag ccatttgtca acgaaagcat acttcagaaa cttatcaata
     1261 ttttcaaggc tgataaggat aaaaacattg atattgtatc accaatgact attctgtcca
     1321 atgagcagga aattcaaaat ccaaataatg taaaggtagt tacagataac gacaggaatg
     1381 cgatgtattt ttcccggagt cctataccct atcatcgtaa tcaatcagca ccacgaaatt
     1441 gctttaaaca tattgggatc tatggatatc ggaaagaagc tctggagaaa atccagaaat
     1501 taccttatag taatttggaa gaccaggaat tgatagagtg ccttagatac ctggattacg
     1561 gtatgaaaat caaaatggca gaaactccgt cccagggagt cagtatcgat actccggaag
     1621 attttgaaaa agcgattgaa ttttataaaa tcttacaatc aggtatcaaa tagtaaatat
     1681 tagcatgtat tgtactaaaa catattaaca atagccaagg attctagttt tgaacagata
     1741 atgcgtaaat aatttgtaac ggttcaggat ctaggctata attaaagcaa acaatcaaaa
     1801 caaagtaggt aggaaactaa tagtcgtgaa tatcttttta aaggaatcgg tgaatgtccc
     1861 ataatataaa acaaataggt gggaatcata tattgattca tattccttcc tctggaatta
     1921 tgccatataa tgaaaaatga taatataata aaaacctgta tctttatggc atgaaattgt
     1981 cagagtcaat tatcatgggt atagacccgg gaacaataaa aatgggattt gccatcatca
     2041 agaatgcaaa acctattcca atattattgg attatggaac tttaaatttc cgacatcttt
     2101 ctgatgctca tgacaaattg catcagatat tcatcaaaat tgaacaacta accaataagt
     2161 atgcggtaaa tgagttagcg atagaagcac cattttatgg aaaaaatgta caatcgatgc
     2221 tcaaattggg acgagctcaa ggtgtggtga tatctgctgc aaaatacttt ggacttgata
     2281 caacagaata ttctccgcga aaaatcaagc aatctgtagc cggcaatggg aacgcctcaa
     2341 aggaacaagt tgcccgcatg atagaaaaac agtttggagt ggatttggga gaagcgggtt
     2401 atgatgcaac ggatgctctt tctgtagcta tatgccatgc attttccaaa aggaacatac
     2461 ttattaaaaa tggaagtaaa aactgggatg attttatcaa aaataatccg gacagaatta
     2521 tttgatcaga gcatcattca atagctgtgg atatgtcttc agccaaggat ttaaattttt
     2581 ccggagttaa ctgaacaacc ggatttttga aattcatatc acttacagta ttgattggca
     2641 tcagatgaac atgcgtgtgc ggaacttcaa gcccgacaac agctacaccg atacgcgtgc
     2701 aaggcactac ttttttcaag gcctttgcca ctttttgtgc aacaagcatc agccctatat
     2761 attcctctga ttcaaggtca aaaatataat ctacctcttt tttgggaatg accagagtgt
     2821 ggcccatcac cataggtctg atatcaagga aagcaaaaaa ataatcatcc tcatacacct
     2881 tataagaagg taattccccg gagataattc tactaaatat acttgccata tctattatcg
     2941 ggacagttta ataattttaa actccaactt tcctgccgga gtagtaatga ttgccgtttc
     3001 tccttctttt ttacccaaaa gtccctggcc aatgggtgag gttccggaaa ttttacctgc
     3061 cttcaggtca gcctcagctt ctgaaaccag agtatatgta aaaattttcc ctgttttcaa
     3121 attttcaatc tcgactgtag acaacaaagt tacaatagtt ggatccaatt gtgaagcatc
     3181 aatcaacctg gcattagcca agactttttc caattcgttg atacgcaatt ccagcatgcc
     3241 ctgagcgttt ttagcagcat catactcagc attttcggag agatctcctt tatctcttgc
     3301 ctcagcaatg gcgcgtgctg cttcctgtct tccggttgtt tttaactcat ccagttcagc
     3361 attcaactta tccaatccat cttgagtcaa atatgtataa tttgccataa tattaatggt
     3421 ttagaataaa aaactaaaca ccccctgctg ggagtgttta gtatatttta caaagttact
     3481 taaaattatt taatataaga aaatatccga cacttagaga ttgattctca cgccaatatt
     3541 atgtgtgcca ctccagactt tggaagccat ataagcataa tctaaagcaa cttttgattt
     3601 tgtctcctta ttgatcgtta aattagctgt agcaccgaat gccaggccgc tatagacatc
     3661 atcctttatc acttctgtag ttttctgaat ttctgcctta taggcaaccc taacctggac
     3721 aagatcttta aaggctgata cttcaacagc tccgccaagc tggtctctgc tgaaggaatt
     3781 agaagtaaaa ttaatcattg gagtcaatct cactgcatca tcagcgaaat atacatcata
     3841 tgacgtacca atattcaaca ccacaggaag ttcatagtta gctgaccttt gatcataagc
     3901 aagattgacg ttttgaacat tagggttagg tcttgttact gtaaaacctt cacctctgaa
     3961 tctcatagga gtacctagat tccgcaatgc caatccaagt ttaaagttat cctttgatcc
     4021 gttggaatat tgcacaccgg catcaaacgc gactccggta gcagatatgc cagggattga
     4081 ttccataaca agacgtacac ccagacctac agaaacctta cctaatgact cctcattaaa
     4141 gaaattatgt gcataagata atccgatatt agtccatgcg acattgaaat tagccccatt
     4201 gccttccggg aaattgactg tagttacagg gatatctcca aagttgactg acatgaaatc
     4261 aaggccgaaa accccttttt tcccaatttt cttagccagt cccaatgcat ttgtgcttat
     4321 acctgttcca accaaatatc gaccatgact ataaccaatc tccatacttt taacactggc
     4381 taatccggct atattaagac gggttgcttc aataccgctg attcctgcag tattcatggt
     4441 atgcagtccg gcacttctag cccatgggtt gattaaaagc tcaccggcgc cagctgaacc
     4501 ctgacgatcg ggattaccgg caaaaccaat ttgtgagaat gctattaagc atattatcgt
     4561 aaataatctt ttcactttaa attctttttt ggtcctaaaa aatgttcgtt ttaatggttc
     4621 cggataactc cggaaccatt tatatcatta gaatcctgat atttcaagct gacggattgc
     4681 cccaacccac ttgattaccc tttctccatc atcagatttg atatggatta gataaatacc
     4741 actaccaaca gtaataccac taccgttctt caaatcccat tcaatgtcag gcgtcacctg
     4801 tgcatatcgc acaccccttg cttcaccact cacttccctt ctgacctcat tcctgttaaa
     4861 ctgtcgaata aacttgccgt caagggaata aatggttacc acacatttgg gcggcagatt
     4921 agttatttta acaatatttg atatttccgt attttcatag gatgaatagg cataataagg
     4981 atttggaaca accgatatct tgtccaaagc acttggagca atagtcggtg attttgtgtc
     5041 ggcagcctta tctttgattt ctatcttata catcggcata ccattattga cacctgttct
     5101 accatctgcc aatgcaggaa ctttataact attattgaca cgaagcttat agacagtctc
     5161 ctgagggatc aatccatctt taaatgacaa catattaaaa ccaagaggta tgcccaccca
     5221 ggtcacctga gcaagaaccc tggacttatt aatatcggaa ggattctgac ctttcaaatt
     5281 gttcagaaaa gtagcacatt cgttgtattt agtctttgtg acgtatatat aatgctgacc
     5341 ccctgcaaag aaattagctg cacttagtaa gttgttacta gcattaataa ataaatttcc
     5401 acccggattg aaaatcatat cctgaccaca attagggtta tcgccgacct cctgaatcag
     5461 atcaccgaaa atagcaggat cataactgga gttctctgca aagaaaatat taagcctcat
     5521 tcctgtctct acatcaattg cataaccagg gaaccaactc atacctgtgg agtcaggatt
     5581 ggcatttaaa acaggattgc catcggcatc aaaatcctta gttatggacc gtgacttacg
     5641 aaggctaaac tgaggtgcac taccaacagg tggaaggcct tctcctgagt acttagtatt
     5701 ggacgcctcg accacaatac actttgacca ttttgatcta tcattggtaa atacaatatc
     5761 gacattattc aaatctgcca aagtatttga tttagatact gtggatgcaa tattggatga
     5821 aggtgggacc ggttgaagaa actcattgac tgccacatcc tgaggctctt ttaacgccaa
     5881 agtcagtggc caaaaaccaa gtttttcata tactccttta ggatccggat taccactaaa
     5941 tttctcaacg taattaatcc aattcaatgc ttcggacaag atcagggaaa tattaggatt
     6001 atctgtaacc ccttctcccc catctcttaa catagaaagc cagttatcct tagtcgcatc
     6061 actgtattgc aat
//
LOCUS       DAOQQN010000185         6068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5597_length_6068_cov_5.982039, whole genome shotgun sequence.
ACCESSION   DAOQQN010000185 DAOQQN010000000
VERSION     DAOQQN010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5597_length_6068_cov_5.982039"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLG24_07845"
     CDS             <1..262
                     /locus_tag="PLG24_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ24840.1"
                     /translation="NTFRLPAYHRGDLSVIYTMSPRWGESNLTLSIYNVYDRRNPYFL
                     TLVTEYKDINQDGVTIKIPEKISARQVSLFPILPSLTWNFNF"
     gene            466..951
                     /locus_tag="PLG24_07850"
     CDS             466..951
                     /locus_tag="PLG24_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24841.1"
                     /translation="MHLQYKRSKLPDENRMLISVDIPDNLDILKKEAISMDDSNMYTA
                     MASIFQEKCEREDIDSESYIDDRWFDVRFISNNTQRRYDLFVSYIFVPENILNEQKLY
                     IAPKVLSKYPSTVYQQGSFQSQLIYLVSPDEKIGFKAVLINERQLEMDKLNRDGENYL
                     S"
     gene            1351..2208
                     /locus_tag="PLG24_07855"
     CDS             1351..2208
                     /locus_tag="PLG24_07855"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HMZ24842.1"
                     /translation="MNKLIPCLALMLTLTSCNLSKEVDIDLPQYTSQPVVECYLIPGQ
                     RYELLLTHSNSFFDPLTVDNPTAYLASLLETGAMVRIIHGNDTVVLEEKVAFNSQTGF
                     ISNYSNEAIVPEDFNGEFSLSITLRNGSQIAANTHIPSPVGLDSIRAEYSNQEKTRAI
                     LFAYYRDNRSSVDYYRRLIHIASLDSVPMQNYVIDDKINTSENVAFGAFYERRDKRPV
                     VGDTLIFSLYHITEEYYRFSQSKDNANIANGNPFGQPGQIKSNVVGPSNPIGIFTGFV
                     PRRDTLILQ"
     gene            3009..4037
                     /locus_tag="PLG24_07860"
     CDS             3009..4037
                     /locus_tag="PLG24_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HMZ24843.1"
                     /translation="MKAVRFVGAKKPLELHEVPLPEIGQGDVLVRVRAAGICHSDVHY
                     RTGIAKMDRYPITLGHEVAGVIEAVGENVGTLHIGDRVCLHYLITCGTCDYCFTGHEQ
                     FCSQANMLGKDVDGGYAEYISVPAKNAVILPDAIPFEEGATLMCASATALHALLKSRM
                     SEGNTVAIFGIGGLGISAVQLAIAMGATAVFAIDLNGDKLALAESYGAIGINPIKTDP
                     VQMIRKLSGGKGADVVLEMIGLPITQNQAIRSASPMGRVVMVGLSDQPVLVDIYREIL
                     GKEIELIGSNDHNLHELSLLMQYASDGRLDTSRVVSRIVPLDAVAINEVLDSLERFDG
                     GVRSVIVP"
     gene            4049..4474
                     /locus_tag="PLG24_07865"
     CDS             4049..4474
                     /locus_tag="PLG24_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HMZ24844.1"
                     /translation="MTDEAYIHRCEELGRSAKENGNPPVGALIVMEDRIISEAEEGGR
                     SKNDITCHAEIEAIRVAVNFLHTNDLSDCILYTSHEPCIMCSYVIRFHRIRKVVFLHK
                     VEYLGGYSSSMALLTGMDVPPSWSKPPEVVCLDNTEEEQ"
     gene            complement(4567..5955)
                     /locus_tag="PLG24_07870"
     CDS             complement(4567..5955)
                     /locus_tag="PLG24_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HMZ24845.1"
                     /translation="MKYFFLFATLIYSFTLSAQEYDRLNIDLLGRWSVPTEKEEEFGT
                     RYSSCWAFEYNGREYAIVGCGNGTAYIDITDPKNPVFIDFVPSNIKDAIWREYKTYKH
                     YAYHVSDDGGPNTFDIVDFSYLPDSVHVVYSGSKEYFRTGHTIFMDSDKMYIGLHNHR
                     SMSVFSMENPVDPKFLRLLSSDYPGDVHDMFVRNDTVYASKGFEGALQIMTFKDDKFT
                     QIGNFTNYPFAGYNHSSWLTPDGKTLIFTDEVPKSLPAKSLDVSDPSNPILLDTFYSQ
                     SGRATLHNPFESPNNTFIMTSYKDGVQVFDYSDPGDIKLRGYFDCYPQQDGEATDGDY
                     DGAWSGYAMLPSKNMIVVDISNGLFVLDATKAYYPETNTVKAATKAIKLETYPNPSTD
                     AFYLKVPEGLKGPMEVRIVTLSGQLVYQNILEASLMAKHRFNTNTYSPGTYIITLKHG
                     KDIYSSEQIIVK"
BASE COUNT         1813 a         1175 c         1375 g         1705 t
ORIGIN      
        1 gaataccttt agattgcctg cttatcaccg aggtgatctc agtgtgattt ataccatgtc
       61 cccacgctgg ggggagagca acctgacgct gagcatttac aacgtatatg accggagaaa
      121 tccatatttt ctcacattgg taacggaata caaggatata aatcaggatg gtgtgacgat
      181 caagattccc gagaaaatta gtgcccgcca ggtttcactt tttccaattc tgccttccct
      241 gacctggaat tttaattttt aagaagtgaa agcaggacga tgaaatcaag tatattcttt
      301 catggtaaga tttgaaaagg agaaggtgca aaagaaggaa tgttgtgttg ggtgaatccg
      361 gcaacttata taatagatga accacgttat gattatctca gaaaatgaat aaatattttg
      421 aggacctacg aggcattgat caaacaggct aacttaatct aaaagatgca tttacaatac
      481 aagcgaagta aattgccgga tgaaaatcga atgctgatat cggtggatat acccgacaat
      541 ttggatatcc taaaaaagga ggcaatctca atggatgata gcaatatgta cacggctatg
      601 gcatcaattt ttcaagaaaa atgtgaaagg gaagatattg attcggaaag ttatatcgac
      661 gacaggtggt ttgatgtccg ctttatttcc aacaacaccc aaagaagata tgacttgttc
      721 gtttcttata tttttgttcc cgaaaatatc ttgaatgaac aaaaactata cattgcccct
      781 aaggtgttga gcaaatatcc atccactgtt tatcagcaag gttcattcca atcacaactt
      841 atctatttgg tttcgccgga tgaaaagatt ggttttaagg cagttttgat caatgaaagg
      901 cagttagaaa tggataaatt gaacagagat ggcgagaact acctgagctg agaactaagt
      961 atgcaggatt tttctataaa tacattaagg cggtatcggg tgttagcaaa tttacttttg
     1021 ttggacgaaa ggtatgacgg caacgaacta taccttgaaa aatgctgctt tttttgcaag
     1081 aacggagaaa tagcatcgtt ggaatcagtg gataatgttg gaatcagaga aatgaagact
     1141 tatatcactc ctaaagtatg tttagtttgg aatccggtga atataacaat tgaaaagcgc
     1201 ttccgttctc aattaatata ccagcttgtt tgacttgaaa ataaatatca tgtattttat
     1261 taaatgtaaa aagtacgatt ataataatgg tctaacacaa tccccaacat cggccatatt
     1321 tcttcacaag attttaaaaa aagtaggaaa ttgaataaat taatcccttg cctggcattg
     1381 atgctgactc tgacttcttg caatctcagt aaagaagttg acatcgattt gcctcaatat
     1441 acatcccaac cggtggtgga atgttatttg attccggggc agcgctatga actactattg
     1501 acacatagca atagtttttt tgatccactg acagtggaca atcccacagc ctaccttgct
     1561 tccttgctgg agacaggggc aatggtccgt attatacatg gcaacgatac agttgtactg
     1621 gaagaaaagg ttgcgtttaa tagtcaaacc ggatttatct ccaattattc caatgaagct
     1681 attgtgccgg aagactttaa cggcgaattt tcactcagca tcaccctcag gaatggcagc
     1741 caaatagccg ctaatacaca tatcccttca cctgtaggcc ttgacagcat tcgtgccgaa
     1801 tacagcaatc aagagaagac gcgggccatc ttgtttgcct attacaggga taacagaagc
     1861 tccgttgatt attacagaag attaatccat atagctagct tagatagtgt accgatgcag
     1921 aactatgtca ttgatgacaa gatcaacacg tcggaaaatg tcgctttcgg tgccttttat
     1981 gaacgaagag ataaaaggcc tgtagtgggc gataccctaa ttttttcgtt atatcacatc
     2041 acggaggaat attatagatt cagtcaaagt aaggataatg ccaacattgc caatggcaat
     2101 ccttttggtc agcccggcca gataaagtcg aatgtcgtcg ggccatccaa tccgattggg
     2161 atattcacag gctttgtacc acgtcgtgat accttgatat tgcagtgagg aactgtggca
     2221 agaagaggta aggtgtggac tctccgaagt aggtaggaaa ataatagcgg gcgaatcggc
     2281 agtgacaggt attggggaga aggtcatcag aaaaaccgtc aattttctga aataattaga
     2341 aaggaatatt ttggagatgc acggtgtccc tcccattgag gttattaatc atctacctac
     2401 tacgattttt gaaaacagta ccccaacaaa ctatgacagg agactataga catgaatttg
     2461 gttgggagta aaagtggcgt gtggcggact atccgaagta ggtaggaaaa taatagcggg
     2521 cgaatcggca gtgacaggta ttggggagaa ggtcatcaga aaaaccgtca attttctgaa
     2581 ataattagaa aggaatattt tggagatgca cggtgtccct cccattgagg ttattaatca
     2641 tctacctact acgatttttg aaaacagtac cccaacaaac tatgacagga gactatagac
     2701 atgaatttgg ttgggagtaa aagtggcgtg tggcggacta tccgaagtag gtaggaaaat
     2761 aatagcggtc gaatcggcag tgacaggtat cggggtgtag gtcatcagaa aaacagtcaa
     2821 ttttctgaaa taattagaaa ggaatatttt ggcgatgcat ggtgtccccc tcttcgaggt
     2881 tattaatcat ctacctacta caaaaaatcc ccgacctttc tgtatttcca tcacccttca
     2941 tgatttatcc tcccaaaggc cgattctttt aagattcata gtatatttga ttggctatat
     3001 tttttgaaat gaaagcagtt agatttgtcg gtgcgaaaaa accattggag cttcatgaag
     3061 tgccgcttcc tgaaattggg cagggagatg ttctggtaag ggtaagagca gccgggatct
     3121 gccattcgga tgtacattat cggacaggta tcgctaagat ggacagatac ccaataacac
     3181 ttggacatga agtggccgga gtgatcgagg ccgttggtga gaatgtcggg acacttcata
     3241 ttggtgaccg tgtctgtctg cactatttga tcacctgtgg cacctgcgat tattgcttta
     3301 cggggcatga acagttttgt tcgcaagcca acatgcttgg caaagatgtc gatggaggat
     3361 acgccgagta catttctgtt cctgcaaaaa atgctgtcat tttgccggat gctataccct
     3421 ttgaggaggg tgccacgttg atgtgtgcct cagcaaccgc cttgcatgcc ttgttgaaaa
     3481 gccgaatgag tgaaggcaat acggtggcca tcttcggtat tggtggcctg ggcatttctg
     3541 cagttcagct ggcaatcgca atgggcgcaa ccgctgtatt tgccatagat ctaaatggag
     3601 ataaactggc attggctgaa agctatggag ccattgggat taaccccatt aagaccgatc
     3661 ctgtccaaat gattaggaag ttgtcaggtg gcaagggagc agatgtcgtc cttgagatga
     3721 tcggcctgcc catcacacaa aaccaggcta ttcgcagcgc ctcacccatg ggtcgtgtcg
     3781 tcatggttgg actgtcagat caaccggtct tagtggacat ctatagggag atactgggta
     3841 aagaaataga acttattggt tctaacgacc acaacctgca cgaattgagc ttgttgatgc
     3901 aatatgcctc cgatggcaga ctggatactt cccgggttgt atcccgtata gttccgttgg
     3961 atgctgttgc gatcaatgag gtattggatt cattggagcg tttcgatggg ggcgtgcgta
     4021 gtgttatcgt gccttaaaag cagagattat gacagacgaa gcatatatac atcggtgcga
     4081 ggaactgggg cgcagtgcca aagaaaatgg aaatcctccg gtaggcgcct tgatagtcat
     4141 ggaagatcga ataatcagcg aggctgaaga aggtggccgc agtaaaaatg acattacttg
     4201 ccacgccgag atagaggcaa tcagggttgc agtaaatttt ctgcatacca acgacctgtc
     4261 agactgtatc ctttatacgt cccatgagcc atgcatcatg tgttcgtatg tcattcgttt
     4321 ccatagaatc aggaaggttg tctttttaca taaggtcgaa tatcttggag gatattcctc
     4381 gtcaatggct ttgttgactg gtatggatgt tcctccatca tggagcaagc caccggaagt
     4441 agtctgcttg gataacacag aggaagaaca ataaatatcc tatgcatgtg gggataccga
     4501 acgtctctgt gcatatcaaa agagaagttc agtaccatgc tatcgttgga tattttctga
     4561 aaagatttac ttcacgataa tctgttctga agaatagata tctttaccgt gtttcaatgt
     4621 aattatatag gttccaggag aataggtatt ggtattgaac cgatgtttag ccatcaagga
     4681 tgcctcaagg atgttttgat ataccagttg gccggacaaa gtgacgatcc gcacttccat
     4741 tggtcctttc aagccttccg gcactttcag ataaaaagca tcggttgaag gattgggata
     4801 ggtctcgagt ttaatggctt tggtcgcagc cttgactgta ttggtctccg gataataggc
     4861 tttggtggca tctaatacaa ataagccatt gctgatgtcc acaacaatca tatttttact
     4921 cggcaacatt gcatagccgc tccaggcacc atcataatcg ccatccgtag cttcaccatc
     4981 ctgttgcgga tagcagtcaa aatagcctct cagcttgata tcgcccggat ccgaataatc
     5041 aaatacctga acaccatcct tgtagcttgt catgatgaat gtgttgttgg gagattcaaa
     5101 tggattatgc aaggtggctc tccctgactg agaataaaag gtgtccagca ggatgggatt
     5161 ggaaggatcc gatacatcca aagactttgc aggaaggctt tttggtacct catcggtaaa
     5221 tatcaaggtc tttccatcag gtgtcaacca ggaactgtga ttataccctg cgaatggata
     5281 attggtaaaa tttcctatct gagtaaattt atcatcctta aatgtcatga tctgcagagc
     5341 cccttcgaaa cctttcgatg catacacggt atcattcctt acaaacatat cgtgaacatc
     5401 tccgggataa tcactggaca aaagtcgtaa aaatttaggg tcgaccggat tttccatgct
     5461 gaataccgac atggaacggt gattgtgtaa cccgatgtac attttgtcgc tatccatgaa
     5521 tatcgtatgt cctgtcctga aatattcctt actacccgaa tatacgacat gtacactatc
     5581 aggcaggtaa gaaaaatcaa cgatatcaaa tgtattaggt ccgccatcgt cgcttacatg
     5641 ataggcgtaa tgcttatagg tcttatattc ccgccaaatg gcatctttga tgttgcttgg
     5701 tacaaaatca ataaaaacgg gattcttcgg gtcagtaata tcaatgtatg ccgtcccgtt
     5761 gccacagcct acaatcgcat attccctgcc attgtattca aaagcccagc aggaactata
     5821 acgggtaccg aattcttctt ctttttcagt aggaaccgac caacgcccca gcaaatcaat
     5881 gttgagcctg tcatattcct gtgcagaaag ggtgaaagag tatataaggg tagcaaaaag
     5941 aaaaaaatat ttcatttcta taaaaattaa atttagtgcg ccaaatgtaa gccttttata
     6001 tcttttaatc ggaagccttg tcgtgaaatt ttgtattgta aatcttaatg atattataat
     6061 tctcaatt
//
LOCUS       DAOQQN010000186         6008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5680_length_6008_cov_5.164623, whole genome shotgun sequence.
ACCESSION   DAOQQN010000186 DAOQQN010000000
VERSION     DAOQQN010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5680_length_6008_cov_5.164623"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PLG24_07875"
     CDS             <1..747
                     /locus_tag="PLG24_07875"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HMZ24846.1"
                     /translation="PSDNLFELLLFIDAAKRASAGYITAVIPYFGYARQDRKDKPRVP
                     ISAKLVANLIQAAGADRIMTMDFHADQIQGFFDIPVDHLKSEAIFTPYLESLDLKNVK
                     FASPDVGGVKRARVYAKYFKKDLVICDKYREKANEVAGMTVIGDVNEADVILVDDLVD
                     TAGTLCKAADALLAKGAKSVRAICTHPVLSGKAFENIEKSSLLELIVTDTIPLTQKSS
                     KIRVLSTSKLFARAIRNTHEHRSISALFVE"
     gene            784..2190
                     /locus_tag="PLG24_07880"
     CDS             784..2190
                     /locus_tag="PLG24_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HMZ24847.1"
                     /translation="MKHVFYFLLPFLFSLSSYPGHSQPFVTGKYQTASEKDILYGQAI
                     NFSGREQPLFMDITYPTDDSPPACGRPLLIAVHGGAFMAGSRTDGNIAGWIRDFAKKG
                     YVAASIEYRLGMFQRPELLQCAIDNYGCLSVADTSEWHRGLYRGAQDVCGAIRYLIAN
                     KEKYNIDPNNVFLTGESAGGFICLEVAYGDDNDPEYTAAAALEKVNKPNAAYNNGCLP
                     PYSYPIDSMNLDRPDLGPRKGTLHPDAGDYRIRGVASFYGGIMNDLFNDVGAVDLPAL
                     YMYAQPNDLVVPFHYGKVMQGVASCAVNSVGCPWIFGNAFSIGNGGIVNFLKNKKAQE
                     FTIPEYQADFTGNSADCILQTLFPQLSGHIIDDYGLRTTNMAKFFASKIDTSCQINAV
                     SHAFDEMNVFPNPAGNQITVRSMEGQNCAINILDLSGKSVISASRFPVNIETLNPGVY
                     FITGIERGRYRMAKFVKK"
     gene            complement(2222..2875)
                     /locus_tag="PLG24_07885"
     CDS             complement(2222..2875)
                     /locus_tag="PLG24_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HMZ24848.1"
                     /translation="MNNYFFLVIESSEIFAQGVKCILAGGFGEGSHVRLIHKKSDLVV
                     PSEVKCIIVGNGLGVEMNFYEILECIKSLRVKAPKLCIPEMITSKMAKALYTDKLVDG
                     IMHRDCSAEEMIHGINVLMRGDVYIGKNIWLHQTKEQSVKATKKDDPFYLVQTLTDQE
                     KLIMEYVIEGKSSNDISQILFRSVHTIKTHRRNLLNKLGVSNTIELITYLDSIGYKK"
     gene            complement(2891..3874)
                     /locus_tag="PLG24_07890"
     CDS             complement(2891..3874)
                     /locus_tag="PLG24_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HMZ24849.1"
                     /translation="MKRRSFIINSGLMLGTGGLDMTAFQQEGPVKGKPIVISTWNFGL
                     EANKEAWRILSANGNALDAVEKGVMVAESDLNNTSVGRGGNPDRDGIVTLDASIMDQH
                     GNAGSVAALEDIEHPISVARLVMEKTPHVMLAGDGARQFALENGFKPIDLLTEKSKAN
                     YKKWLETSKYKPVINIENHDTIGMLALDASGNFSGACTTSGLAFKMHGRVGDSPIIGA
                     ALYVDNNYGAAVCTGIGELVMKNLSSFLAVEQMRSRKSPQQACEYAIRRIMEKTPDVS
                     NAQVGILAINRKGEIGACAIQEGFTYAVRSVDVEKVFNAAHRYKRSKSTSQ"
     gene            complement(3898..5916)
                     /locus_tag="PLG24_07895"
     CDS             complement(3898..5916)
                     /locus_tag="PLG24_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ24850.1"
                     /translation="MKILTVPILILLTMAASTVLAQPLKTIPYQARIEKADEYADRNN
                     YASQLDELEEVYKERKDKSMLPLMAQLNYKLRDYGRAENLYKRIVDGDKKGTDIEARY
                     MYGKMLKLNGKYQEAAEVLREVKNTDDDVYAPLAIMELKGILLAVSTDTVKQISVVPA
                     GPKINTKSGEYSPTLYNNELYFTSTALAAGEEPGNTYVVKLLKSQRDKDGGWSKASEV
                     DALISASSPAVGNVAFSQADQIMYLTKVTFDGEEVATSRVYYSKREGDAWTDPALVKG
                     LPDSFIVKNPMPGELYGRDVLFFSSNAPGGRGGFDIFYATKISEGIFDSPVNLGPTVN
                     TAGDEITPFYKEGKLVYATDGLPSYGGFDLYSTEWNGTSWSTPANMGPGFNSPADDMY
                     YTIDEEGYEGLLVSNRVGTTSLRGKTCCDDIFTFSIAKPVVELEVYVLDENKPLRHGE
                     LSIMEQFKPETKVTEGKDDTYHFSYELDINKGYFIKVTRIGFFPDSLYVKTTGIKEDS
                     TMIVKINLKPKPEVEVISINQPIRLNNIYYNYNDAKILKAAMPDLDYLYELMQQYPDM
                     VIELSSHTDSRGNDEFNMKLSQRRADSAMNYLLAKGIAPERIQAVGYGESMLLNNCGN
                     DVKCSEEEHQLNRRTEFKIISGPTNIEIKKQITRERGKIIDTKTIKEK"
BASE COUNT         1587 a         1399 c         1294 g         1728 t
ORIGIN      
        1 ccatccgaca acctgtttga actcttgctt tttattgatg ctgccaagag ggcctctgca
       61 ggatatataa cggctgtaat tccttatttc ggctatgcac gtcaggatcg caaagacaaa
      121 ccaagggttc ccatttcggc caaactggtg gccaatctga ttcaagcggc aggtgccgac
      181 cggatcatga ccatggactt ccatgccgat cagatacagg gattctttga tattccggtg
      241 gaccatctca agtcggaagc gatttttaca ccataccttg aatccttaga tcttaaaaat
      301 gtcaaatttg cttcacctga tgttggtggg gttaagcgtg ccagggtcta tgccaaatat
      361 ttcaaaaaag atctggtcat ctgcgacaaa taccgtgaaa aagcgaatga agtcgccggt
      421 atgaccgtca taggtgatgt aaacgaagcg gatgtcatat tggttgatga ccttgtagac
      481 actgcaggca ccctttgcaa ggctgccgat gcattacttg ccaaaggtgc caagagtgtc
      541 agggctatat gtacacaccc tgtcctgtca ggaaaggcat ttgaaaatat cgaaaaatcc
      601 agtctccttg aattgattgt gaccgatacc ataccgttaa cccaaaaatc ttctaaaata
      661 cgggtattgt ctacgagcaa gttatttgcc cgggcaatcc ggaacactca cgaacacaga
      721 tcaatctccg cacttttcgt agaataaagg ataaaatttc ataaccagca gaaaaccttt
      781 aaaatgaagc acgtttttta ctttctgctc ccctttctat tcagcctgag ttcttatccg
      841 ggacacagcc agccgtttgt aaccggtaaa taccagacag cctccgaaaa agatatcctc
      901 tatggacagg caatcaattt ttcaggcagg gaacaacctc ttttcatgga catcacttat
      961 cctacagacg actcacctcc ggcatgtggc aggcctttgc tcatagccgt ccatggaggt
     1021 gcctttatgg ccgggtcgag gacagacggg aacattgcgg gttggatcag ggactttgct
     1081 aaaaagggtt atgttgctgc ttccatcgaa tatcggttgg gtatgtttca aaggccggaa
     1141 ctgcttcaat gtgccatcga caactatggc tgcctgagtg ttgccgacac ttccgaatgg
     1201 catcggggct tgtatcgggg agcacaggat gtatgtggag caatcaggta tctgatagca
     1261 aataaggaaa aatacaacat cgacccaaat aatgttttcc tgacaggaga aagtgccgga
     1321 ggcttcattt gccttgaagt ggcatatgga gatgataatg atccggaata cacggctgca
     1381 gcagcattag aaaaggtcaa caagccgaat gctgcctata ataatggttg ccttccaccc
     1441 tatagttatc cgatagattc catgaacctc gatcggcctg atttagggcc cagaaaaggt
     1501 accctccatc cggatgcagg cgattacagg attcggggtg ttgcctcctt ttacggtggc
     1561 attatgaatg atcttttcaa tgatgtcggt gctgtggact taccggccct ttacatgtat
     1621 gctcagccga atgatttggt ggtacccttt cattatggca aggtgatgca aggtgtagca
     1681 agctgtgccg taaattcggt cggatgtccc tggatcttcg gcaacgcatt ttccattgga
     1741 aatggaggca ttgtcaattt tttgaaaaat aaaaaagcac aggaatttac cattccggaa
     1801 taccaggctg atttcacggg caattcagcc gactgtattc tccagacgct ttttccgcaa
     1861 ttatccggcc atatcattga tgattacggg ctcagaacaa cgaatatggc caaatttttt
     1921 gcctctaaaa tcgacacgtc atgccaaatc aatgctgttt cacatgcttt cgatgaaatg
     1981 aacgtatttc caaaccctgc cgggaatcag attactgttc gttcgatgga aggccaaaat
     2041 tgtgccatca atattctcga cctctcagga aagagcgtta tatccgcttc ccgatttcct
     2101 gtcaatattg aaacattgaa tcccggagta tattttatta cagggataga gcggggaagg
     2161 taccggatgg caaaatttgt aaaaaaataa gatcaacaga cgttcctgtc agggccgatc
     2221 tttacttctt atacccgatt gaatccaggt aggttatcaa ttcaatggta ttggagaccc
     2281 ccagcttgtt gagcaggttg cgccggtgtg tcttaatggt atggactgac ctgaacagga
     2341 tttgggagat atcatttgac gacttaccct caataacata ttccatgatc agcttttcct
     2401 ggtcagtaag ggtctggacc aggtagaatg ggtcgtcttt tttcgttgct ttcacacttt
     2461 gctccttggt ctgatggagc cagatatttt taccgatata tacatctcca cgcatcaata
     2521 cattgattcc gtgaatcatt tcctcagcag aacagtcacg atgcatgatg ccgtccacta
     2581 atttatccgt ataaagtgcc ttggccattt tggaggtaat catctcagga atacaaagct
     2641 ttggtgcctt aacacgcaag gacttgatac attccagtat ttcgtaaaaa ttcatctcga
     2701 caccaaggcc attgccaacg atgatacact taacttcaga tggaacaacc aagtcacttt
     2761 ttttatggat cagacgaaca tgagaacctt ccccaaaacc gcctgccagg atacacttca
     2821 caccctgagc aaatatttca ctgctttcga tgactaagaa gaagtagttg ttcatgggtg
     2881 gaggttttta ttattgtgaa gtcgatttgc ttcttttata gcgatgggca gcattaaaaa
     2941 ctttctccac atcaacagaa cggactgcat aggtaaatcc ttcctgaatg gcacatgcac
     3001 ctatctcacc ttttctattg atggcaagga taccgacctg agcattgctg acatccggtg
     3061 ttttttccat gatgcgcctg atggcgtact cgcaagcctg ttgaggggat ttgcgactgc
     3121 gcatctgctc aacagccagg aaggaagaaa gattcttcat gaccagttca ccaatcccgg
     3181 tgcatacagc agcgccatag ttattatcga catacaaggc ggcccctata atcggtgaat
     3241 ccccaaccct gccatgcatc ttaaatgcca aaccgcttgt tgtacaagcg cctgaaaaat
     3301 ttcccgaagc atcaagggca agcatgccga tggtgtcgtg attttcgata ttgatcacgg
     3361 gtttatattt ggatgtttcc aaccatttct tataattggc tttacttttt tctgtcagca
     3421 gatcaatggg tttgaagcca ttttcaaggg caaactggcg tgcgccgtca ccggctagca
     3481 tgacatgggg tgttttctcc atgaccaatc tagccactga tatcgggtgt tcgatgtctt
     3541 caagggctgc tacagaaccg gcattaccat gttgatccat gatgcttgca tcaagggtca
     3601 cgattccatc ccggtcggga ttgccgccac gtcctaccga agtattgttg aggtcagatt
     3661 cggctaccat cacccccttc tccactgcat cgagggcatt gccattggct gacaggatac
     3721 gccatgcttc cttgttggct tcaagaccga aattccaggt agagataaca atcggctttc
     3781 ctttgacagg gccctcctgt tgaaaagctg tcatatcaag accaccggtg ccaagcatca
     3841 ggcccgaatt gatgataaaa ctccttctct tcatgacaag cttttaatat caaagaatta
     3901 tttttcctta atggttttcg tatcaatgat ttttcctctt tcccgtgtga tttgtttctt
     3961 aatttcaata ttagtcggtc cggaaataat cttgaattca gtacgcctgt tgagttgatg
     4021 ctcttcctca ctgcacttga catcatttcc acagttgttg agcaacatgg attccccgta
     4081 tcccaccgct tgtattctct ccggagcaat gcctttggcc aacagataat tcatggcaga
     4141 gtccgctcta cgctgggaca atttcatatt gaattcatca ttaccacgcg agtcggtgtg
     4201 tgaggacaat tcgataacca tatccggata ttgttgcatc aattcgtaca aataatccaa
     4261 atcaggcatt gcagccttca gaatttttgc atcgttataa ttataataga tgttgttgag
     4321 acggataggc tgattgatgc taattacctc aacttcaggt ttcggtttta ggttgatttt
     4381 gacaatcatg gtactgtctt ccttgatacc ggttgtcttt acataaagag aatcggggaa
     4441 aaatccaatc ctggtcacct taataaaata acctttattg atatccagtt cataagaaaa
     4501 atggtaggta tcatcctttc cttctgtaac ttttgtttcg ggtttaaact gctccataat
     4561 ggacaattca ccatggcgca atggtttgtt ctcatcgagg acatatactt ccagttctac
     4621 cacaggcttt gctatggaga aggtaaagat atcatcacag catgttttgc cgcgtaatga
     4681 ggtagtgcct accctattgg ataccagcag gccttcgtaa ccttcttcat cgatggtata
     4741 gtacatatca tcagccggag aattaaaacc cggacccata ttggcaggag tggaccagga
     4801 ggtgccgttc cattctgtgg aatacaggtc gaaaccaccg tatgaaggaa gtccgtctgt
     4861 tgcatacacc aattttcctt ctttataaaa gggcgtaatt tcatcgcccg cagtattgac
     4921 agttggtcca agattgaccg gtgaatcgaa tatgccctcg gaaatcttag tcgcatagaa
     4981 gatatcgaat cctcctcttc caccgggggc attagaggag aagaacagta catctctgcc
     5041 gtataattct cccggcattg gattcttgac gataaaagaa tccggcagac ctttgaccag
     5101 tgcgggatca gtccatgcat ctccttcccg cttactgtaa tacaccctgg atgtagctac
     5161 ttcttcgcca tcaaaggtaa ccttggtcag atacataatc tgatcggctt gcgaaaaggc
     5221 cacattgccc acagccggac tggatgcaga tatcagtgca tcaacctcag aagccttact
     5281 ccatcctcca tccttatccc tttgtgactt cagcaatttg accacataag tatttccggg
     5341 ctcttcaccg gcagcgaggg ctgttgaggt gaagtacaat tcattgttgt atagagtggg
     5401 tgagtattct ccgctttttg tattgatctt gggtcccgca ggcactacgg atatttgctt
     5461 tacggtatcc gtacttactg cgaggagaat acccttgagt tccatgatag caagaggggc
     5521 atatacatca tcgtcggtat tcttaacctc tctgagcact tctgcagctt cctggtattt
     5581 gccattgagt tttagcattt taccatacat ataccgggct tctatatccg tacctttctt
     5641 gtcgccatcc acaatccgtt tatacagatt ctccgcacga ccatagtctc tcagcttata
     5701 attcaattgc gccatcagag gcaacatgga tttgtccttt ctttccttat acacctcttc
     5761 cagttcatcc agttgactgg cataattatt cctgtcggca tattcatcag ccttctcaat
     5821 gcgagcctga tacggaatgg tcttcaatgg ctgggccaac acagttgaag ctgccattgt
     5881 caacaaaatc aatataggaa cggtcaatat cttcataggg aatgagatta aattctggga
     5941 caaaggataa ccgggttgtc cttcggtttt ttatagatca taaaaatatg gctgactgaa
     6001 atctcaag
//
LOCUS       DAOQQN010000187         5992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5708_length_5992_cov_5.390601, whole genome shotgun sequence.
ACCESSION   DAOQQN010000187 DAOQQN010000000
VERSION     DAOQQN010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5708_length_5992_cov_5.390601"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="PLG24_07900"
     CDS             complement(<1..1018)
                     /locus_tag="PLG24_07900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HMZ24851.1"
                     /translation="MHFLFPAFLIASLVVSIPILIHLFNFRRYKKVFFSNVAFLKSLQ
                     QASNSRKKINRWLILAARCLALLALVFAFAQPYFETKGKVERGTNYSVIYLDNSFSMG
                     NKASEGNLLEQGKESAREIINGFGPDDKFQVITNDNDGFRQSWLNKDEALASVVEKSI
                     SPQFKPMSAIFRSMQQSFKFSGSGKHNAYFISDFQRAATDLSAIRDTGIMTHLIPMRS
                     ATISNVYIDSVYFKDQVSVPGVADRLIYKIKNDGQAIHTIKPVLKYNGNSKPLPIVEL
                     AAGKEYTDTVSLLALKPGFQSAELIINDAPITFDDNYHIAWDAVGKIRALVINEGNQN
                     RYLTS"
     gene            1113..1334
                     /locus_tag="PLG24_07905"
     CDS             1113..1334
                     /locus_tag="PLG24_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24852.1"
                     /translation="MEQWINGIGVTMILLAFILLTVKKISSDSLTYKLLNFLGAGLAC
                     IGAWLVGIKAFVILEGTWSVVALISIFKK"
     gene            2338..2826
                     /locus_tag="PLG24_07910"
     CDS             2338..2826
                     /locus_tag="PLG24_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24853.1"
                     /translation="MKYFILILFFLGIYQTGTSQNAEEYFSYLEGLENSGQHSQAEYL
                     RGLYKNLVPTMYFDDGELKTYGPPYPQRLLTNNRYVYLGEKAIVSNYDMSNVEIIIIK
                     YDEGENIQQLLLDPAFVQSLTALKYIVFVTSYNAQISKFDEIIPQIPANVKRLVRISI
                     EE"
     gene            2851..>5992
                     /locus_tag="PLG24_07915"
     CDS             2851..>5992
                     /locus_tag="PLG24_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24854.1"
                     /translation="MKNKIYKSELLNWLGKLSIIIFLAISFSIKSEAQLVVPFAKTPD
                     QIFSVKGDYTMFGNSLLIKSPYSNDGQNGNNDMMYVDIDTDVNTWNSSSANFDFAVEP
                     GVNPNCTQILYAGLYWTGRANNGGTAGNTVDATRFYNGNMENRTFTKNHIKLKFGTDS
                     YQNITGNFIANPPTDYYQIYSCYADVTGYVQSKGAGTYTVADMALVQGNADGVGYFGC
                     WSMVVVYQNSKMKTKNIAIFQGHAFNSTNNIVDIPIVGFQAVQSGDVNVKLGFMAGEG
                     DRDLIGDYAKIQELNSGTFKSLSHSGNSTSNFFNSSINTGGNARNPSYLNNYGIDVAM
                     FYLDNGNDNDPGTPPVNNVIGNGQTSTIIRVGTTSDIYTMSVLVFGVDAYQPETDEII
                     KLTAIDGNPPVVPYTCTPGQELTYCVDIKNVGTEPVKNYKLSVPIPSNTDYVTNSVTA
                     TVHPLAGTPNTPKPIYNASTRSLEWDFGNLPQPVDPTTILASFCFKLKATTNCLLLSN
                     SCGQDIPVTGYANGTGVITNTHIVNQKFILGYVDSGECTNEKINGNLVTHIDGAAYVT
                     SNCDANLQDGILNLEIVQNPSGYPVANVAPYFPPGTKFYSAYPVVPGTIEYNASNPFP
                     AVDGTTKTYWAVPPGPNACPIKIILTCVNNCSFSVNCGNQPSGNYDCKRPVPGAVSSL
                     SAFNSTFGASQGPNPCHPIKMKVIEDSAFNPCTSLEYKRTYRIYQDNNGNNQYDSGEP
                     FVDCIKSYIWLRDQTKPHYSCDPATPVLIGCRNNGPTAQDAITAAGFTDNCTATNQLT
                     IVATPLDITTNVACYKIQNWKVEATDQCGNYAQVIVQVKWTTDTQAPVFADPNDKTID
                     LGCNPTAPNAAKAITDAGAATDNCSVSVTASAGSVTDTCKKTQVWTVKAKDDCDNTTT
                     KKVTYTWTSDTQKPTFSNCQSGQNIILDGALPPTPEDAIAFVGTISDNCTPYANLVIT
                     ATGGQIVPSGGTGCKFTQNWTVSATDECGNTKTCTFGLIWGSDTEAPVFANCPTGKIL
                     LGCNPTKPTTNDAIANA"
BASE COUNT         1885 a         1227 c         1140 g         1740 t
ORIGIN      
        1 ctgatgtaag ataacgattc tgattgccct cattgatgac caaagcccga atctttccta
       61 ctgcatccca ggcgatatga taattgtcat cgaaggtaat cggtgcatca ttgataatca
      121 actccgcaga ttgaaatccc ggctttaatg caagtaagga aacagtatcc gtatactcct
      181 tcccggctgc aagttcgacg ataggtaacg gtttggagtt tccattgtat ttcaatacag
      241 gcttgattgt atggatggct tgaccatcgt ttttaatttt ataaatcagg cggtcagcaa
      301 cacccgggac agaaacctgg tccttgaaat acaccgaatc tatatataca ttagatatag
      361 ttgcagatcg cataggtatc aggtgcgtca taatgcctgt atccctaatg gcagaaagat
      421 cagtggcggc ccgctgaaaa tcactgataa aataagcatt gtgtttgccg gatccggaaa
      481 atttaaaact ttgctgcatc gaccggaaga tagcgctcat gggtttgaat tgtggggaaa
      541 tgcttttttc taccacactc gccaatgcct cgtccttatt gagccaggac tgcctgaaac
      601 cgtcattatc attggtgatt acctgaaact tatcatccgg tccaaaaccg ttaatgatct
      661 cgcgtgctga ttccttaccc tgctcaagca aatttccttc ggacgcctta ttgcccatag
      721 agaagctgtt gtccagatat atcacggaat aatttgtccc acgttctacc ttcccctttg
      781 tctcaaaata tggttgagca aaggcaaata ccagagccag gagagccaga caccgtgcag
      841 ccagaatcag ccatctgttt atcttcttgc gactattgga agcttgttgt aaggatttta
      901 aaaaagcaac attggagaaa aacacctttt tatatcgtct gaaattaaaa aggtggataa
      961 gtatcggtat ggatactacc agtgaagcta tcagaaaagc aggaaataaa aaatgcatcg
     1021 ccaaaagtat aattttatac ggatattgaa cattccaatt attaacgtat aacaatcatc
     1081 caatgctata tttgttcaaa aattcattaa aaatggagca atggatcaat ggtatcggag
     1141 tcacaatgat attgttggct tttatattac ttactgtaaa aaaaataagt tctgatagtt
     1201 tgacatacaa attactgaat ttcctgggag ccggtctggc gtgtattggt gcctggttag
     1261 tgggcataaa agcatttgtt atccttgaag gtacatggag tgtggttgct ttaatttcaa
     1321 ttttcaaaaa atagttacca aatatcacct aaaaagtagt gaagcatggc agtctcgata
     1381 aacaaatgac cggaccggaa gaacaattag taatgaatgg atacgaatgc ctgctaaaaa
     1441 accagatact ctctctgcaa attgaagaat attgacataa aaacatcgtg ttgtaaataa
     1501 aaaacattca aaattcacat ttcaaaaaac gccccttcat tgaccgcatt catatttata
     1561 tatatcatac aagaatgtat atatgtctga tacaaatgat gattcaatgt aaaaaataat
     1621 ataatacatg aaaatagagc cgattccggt cgcttcttga caattaattg tagtaaaata
     1681 tctacatcgt ttcattttta tcaattaata ataataaaat atgttgcaat taaaataaca
     1741 tatacatctg tttatctgat acttacatca taaacatgaa tatcctatta taataaaata
     1801 gatttgattt ttttacaaaa attaatttat ttttatacac ccttataaaa aacagacaaa
     1861 ttttttaata ttttttatat ttaaatagtc atatatattg aaataattct acataaagta
     1921 cgaatcctta aaatttttct tgcatgccat attatctttg ccccaacgat tgaaattgtt
     1981 ttttgacaca cactatttaa atttcaatgt agcaacagac cataaaagtc tgaagcattc
     2041 cgaaacctta gagaactaca aaatgtatga tatgatactt atatagtatt tcaaatccat
     2101 ctatacttac ctgacatttt tatagtgtgt ggcaaaagtc aaaataaacg cgacacacac
     2161 tatgattttt ttctttccac ttcaaagcta ctgttttagg cagtaagatt tgccaaccac
     2221 aaccaaaagg atatttatgc atatccggag gggtggttgg gtttcctgag tgactgattt
     2281 agcatggttt gtgtgtttga ttgtatgatt tttatctcaa ttaaaccata tttaactatg
     2341 aagtatttta ttttaatttt gttctttttg ggtatttacc aaacaggaac aagccagaat
     2401 gcggaggaat acttttccta cctggaaggg ttggaaaatt ccggacaaca ttctcaggcg
     2461 gaatacttga ggggactcta caagaatctt gttccgacaa tgtatttcga tgatggggag
     2521 ttaaaaactt atggtccgcc ctatccgcaa agactgctta ccaataatcg ctatgtttat
     2581 ctgggagaga aagcaattgt ctccaactac gacatgtcaa atgtcgagat tatcatcatc
     2641 aagtatgatg aaggagaaaa catccagcaa cttctcctgg atcctgcctt tgtgcagagt
     2701 cttaccgctt taaagtatat cgtctttgtg acgtcataca acgcgcagat tagcaaattc
     2761 gacgaaatta ttcctcaaat tccggccaat gtcaagaggc tggtaaggat ctctatcgaa
     2821 gaataatatt aagcattaaa attggtaacg atgaaaaata aaatatacaa atccgagctc
     2881 ctaaattggc tgggaaaatt atccatcatt attttccttg caatttcttt ttcgataaaa
     2941 agtgaagctc aattggtggt tccatttgcc aagacacctg atcagatatt tagtgtcaag
     3001 ggagattaca ctatgtttgg caactccttg ctcattaaat ctccatattc aaacgatgga
     3061 cagaacggga acaatgatat gatgtatgtt gatattgaca ctgatgtcaa tacatggaac
     3121 tcatcatctg caaattttga ttttgccgtt gagccgggtg tcaatcctaa ttgtacgcaa
     3181 atcctgtatg ccggtcttta ttggacaggc cgggcaaaca atggaggcac agccggtaat
     3241 accgttgatg caaccaggtt ctacaatggt aatatggaaa acagaacatt tacaaagaat
     3301 catatcaaat tgaagtttgg taccgattct taccagaata ttaccggaaa cttcattgca
     3361 aatcctccta ccgactatta tcagatatac tcctgctatg cggatgtaac aggttatgtc
     3421 cagtccaagg gcgcaggtac ctatactgtt gccgacatgg ccctggttca gggtaatgca
     3481 gacggtgtcg gttacttcgg ttgctggtcc atggttgttg tatatcagaa cagtaagatg
     3541 aagactaaaa acatagccat cttccaaggc catgcattca actctaccaa taacatcgtg
     3601 gatattccta tagttggctt ccaggctgtt cagagtggtg atgtcaatgt taaactaggc
     3661 ttcatggcag gagaaggtga ccgtgacctg ataggagact atgccaaaat acaggaattg
     3721 aattccggca ccttcaaaag tttgtcacac agtggtaatt caaccagcaa cttcttcaat
     3781 tcatccatca atacaggcgg caatgccaga aacccgagct atcttaacaa ctatggtatt
     3841 gacgttgcaa tgttctatct ggacaatggt aatgacaatg acccaggtac tcctccggtt
     3901 aataatgtaa tcggcaacgg acaaaccagt acaatcatac gagtaggtac aacaagtgat
     3961 atttacacga tgtccgttct ggtatttggt gtggatgcat atcagccgga gacagatgaa
     4021 attattaaat tgacagctat agatgggaat cctccggttg taccttatac atgtactccc
     4081 ggacaggaat taacctattg tgttgatatt aaaaacgtag gtaccgagcc ggtgaagaat
     4141 tataaactct ctgttccgat accttccaac actgattatg tcactaacag tgttactgcg
     4201 acagtccacc ctttggccgg cactccaaac actccaaaac cgatttataa tgcttcaacc
     4261 agatcactgg aatgggactt tgggaacctg ccgcagccgg ttgacccaac caccattttg
     4321 gcaagtttct gtttcaaatt gaaagcaaca accaactgtc ttttattaag caatagttgt
     4381 ggacaggata tacctgttac cggatatgcc aatggaacag gtgtaataac caatactcat
     4441 attgtcaatc agaaattcat ccttggctat gtagattcag gtgagtgtac caatgaaaag
     4501 atcaacggta atctcgtcac ccatattgac ggtgcagctt atgtcacctc caactgcgat
     4561 gcaaatcttc aggatggaat tcttaatctt gaaatagttc agaatccttc aggctatccg
     4621 gtcgccaatg tggcacctta tttccctccg ggaacaaaat tctactcagc ctatccagtc
     4681 gtaccaggaa ctattgaata taatgctagt aatccatttc cggcagttga cggtactaca
     4741 aagacatact gggcagtacc tcccggaccg aatgcttgtc caatcaagat catattgact
     4801 tgtgtcaaca attgttcttt ctctgttaat tgcggcaatc aaccttccgg aaattacgat
     4861 tgtaaacgtc ctgtaccggg agccgtttct tccttgtctg cattcaactc tacatttggt
     4921 gcatctcaag gaccaaatcc atgccatccg atcaaaatga aagtcattga ggattcggca
     4981 ttcaatcctt gtacttcgtt agagtataag cgtacatatc gtatctatca ggataacaat
     5041 ggcaataatc aatatgacag tggtgaacca tttgtcgatt gtatcaaatc atatatttgg
     5101 ctcagagacc agacaaagcc gcactattca tgtgatccgg ccacacctgt gcttattggt
     5161 tgtagaaaca acggaccaac cgctcaggat gcaatcactg cggcgggatt taccgataac
     5221 tgtacagcaa ccaatcaact gaccatagtt gcaaccccac ttgatattac aactaatgtc
     5281 gcttgttata aaatacaaaa ctggaaagta gaagctactg atcaatgtgg caactatgca
     5341 caggtaatcg ttcaggttaa atggactacc gatacccaag ctcctgtatt tgctgacccg
     5401 aacgacaaga ctattgacct gggctgcaac cctacagcac cgaatgctgc aaaagccatt
     5461 actgatgcag gagctgcgac cgataactgt agtgtttcag ttactgcctc agcaggtagt
     5521 gtcacagata cttgtaaaaa gactcaagtt tggactgtca aggcaaagga tgattgtgat
     5581 aacacaacta ccaagaaggt tacctatacc tggacgagtg atacccagaa accaaccttc
     5641 tccaattgcc agtccggaca gaacatcatc cttgatggtg ctttacctcc aactccggaa
     5701 gacgccattg cctttgttgg tacaatttca gacaattgta caccctatgc caacctagta
     5761 attactgcca caggaggcca aatagttcct tcaggaggaa ccggctgtaa attcactcaa
     5821 aactggactg tatcagcgac tgatgaatgt ggaaatacaa agacttgtac atttggtttg
     5881 atttggggtt ctgatactga agctccggta tttgccaact gtccgacagg taaaatcttg
     5941 ttgggttgta atccaaccaa acctactaca aatgacgcca ttgccaatgc cg
//
LOCUS       DAOQQN010000188         5980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5735_length_5980_cov_4.550042, whole genome shotgun sequence.
ACCESSION   DAOQQN010000188 DAOQQN010000000
VERSION     DAOQQN010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5735_length_5980_cov_4.550042"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="PLG24_07920"
     CDS             <1..1210
                     /locus_tag="PLG24_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HMZ24855.1"
                     /translation="YFVYFAVLIGVVGVTISTLIGKFGAGWYLLYPLPFKGTFWPGWS
                     TLLIIISLVILGVAWLIGNIHVVFALAREYGGLGNILGWQYFGKKEPERELPPVVLIS
                     AVSLIPGILAFIAGAIFLIMYLLQNFEPGLTFDNLLVKNLIFFFGHTLVNITLYCCLG
                     WVYTIMPEYTHREWKVNKLLVWAWSGTFFFILFAYFHHLYMDFAQPKGMQYAGQLASY
                     FSAIPATAVTMFGMIAQVYHAKLKWSIVPLAFLMGVAGWAIGGFAAIIDSTIALNKIL
                     HNTLWVPAHFHTYMLGGVVLFILGYLFYITHSKEELLKNNMAKFGFWSFLLAAHGFLA
                     FFYSGGLYSIPRRYSDYKGIPMEETHNIGANIAAVSTIFISFVLIGLLIMYVSLFKKI
                     LAKNTQDGII"
     gene            1255..1878
                     /locus_tag="PLG24_07925"
     CDS             1255..1878
                     /locus_tag="PLG24_07925"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HMZ24856.1"
                     /translation="MRNVFIPILSLIICLTVIGVWTLGFSSFTIFSYTLKKAGELPRP
                     LPDIQMINHEGIVFNPGEIKKYKLINFVYLNCPFVCHKINNRLEQIYNKIDTNLVPNE
                     IEFLTVSFDLKNDDVKKIKNYHKLFKGEISGWTFALPYQSTDRYFTDFLSKLGIWKYT
                     VPSTGLINHSIYIFLVNRQNQIVRVFDPARESDEMIIQGITQCLKEK"
     gene            1908..2417
                     /locus_tag="PLG24_07930"
     CDS             1908..2417
                     /locus_tag="PLG24_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24857.1"
                     /translation="MIWLLSFTLNEWLSETMPRHQLIQLPSMFFIGVLMGITASHHLK
                     PDLPWSIAVLIFVMFSLLFWMIPHSIDYAVISPGFNRIMHANMLLAGFLTISSIRHTI
                     PEVKIIFLGMVAAMIIAGGIALLSFNILLCSAFTIEQQKQTGTLLTLLGLALYFFMAF
                     ILFSSLSKK"
     gene            2994..3227
                     /locus_tag="PLG24_07935"
     CDS             2994..3227
                     /locus_tag="PLG24_07935"
                     /inference="COORDINATES: protein motif:HMM:NF022969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HMZ24858.1"
                     /translation="MENKDIWLESLITATPQEGRELAIKMARKTIAAIQTDPEIRKKL
                     RSDYANDTTQLIASANVVAIEFQTIAQANNYWK"
     gene            3408..4178
                     /locus_tag="PLG24_07940"
     CDS             3408..4178
                     /locus_tag="PLG24_07940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ24859.1"
                     /translation="MEITDFAKAQGHWILAKMGKRVLRPGGKELTKKLVSELHIQHDD
                     DLVEFAPGLGYTANLALHHAPHSYIGVDADEDAVRELKNRIKGRNTKFQIGNAAETHL
                     PDESKDKVFGEAMLTMHADHRKSDIIKEARRILKKGGLYAIHELGLVDVDDTLKSEIQ
                     KELALAIKVNARPLTEEEWRALLEKEGFKVNKVYTNGMFLLEYRRILEDEGFFRTLKI
                     GFNIITHPKALKRILGMKKIFRKYQRHMNAIVIISEKI"
     gene            4197..4913
                     /gene="ric"
                     /locus_tag="PLG24_07945"
     CDS             4197..4913
                     /gene="ric"
                     /locus_tag="PLG24_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HMZ24860.1"
                     /translation="MNITKESIIGDLVAHDYRTASVFKKNKIDFCCNGNRSIEEACDK
                     KNIESKGIIEQLQAATAQAASSGIDFNAWPLDLLADYIEKTHHRYVENKIHEIMPFLE
                     KIVRVHGDRHPELAEIAQLFKESAEELSAHMKKEELVLFPYIRQMVLARQRGETVSAH
                     FGTIQNPIRNMMHEHDTEGERFRKISDLSNNYTPPADGCTTYKVTFSMLKEFEDDLHL
                     HIHLENNILFPKSIELEMAF"
     gene            4956..5480
                     /gene="azu"
                     /locus_tag="PLG24_07950"
     CDS             4956..5480
                     /gene="azu"
                     /locus_tag="PLG24_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HMZ24861.1"
                     /translation="MKKIIFLFAVTLFFASCGGNSSAPATDETATEATAEEGGEEATA
                     DEADANIAEVTIKGDDAMKYDLSEIKVKEGQTVHLTLVHSGKAPVAAMGHNWVLMKPG
                     TDVEKFATAAINAKDNDYIPADMAADVIAHTKTIGGGETTDVEFTAPAKGTYDFLCSF
                     PGHHATMKGKFIVE"
     gene            complement(5729..>5980)
                     /locus_tag="PLG24_07955"
     CDS             complement(5729..>5980)
                     /locus_tag="PLG24_07955"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA
                     nucleotide-binding domain protein"
                     /protein_id="HMZ24862.1"
                     /translation="GALVIQKDRILKASCILPVSDNINVPPSAGLRHRAALGISEVSN
                     AKTFIVSEETGRMSYTARGIIEMNVDEERILNLLIEALK"
BASE COUNT         1850 a         1185 c         1194 g         1751 t
ORIGIN      
        1 ctattttgtt tattttgcgg tattaatagg tgtagttggg gtcaccatct ccaccttaat
       61 cggaaaattt ggtgccggct ggtatctttt gtacccgctt ccattcaaag gaactttctg
      121 gcccggctgg tccacccttt tgatcatcat ttctcttgtt attttaggag ttgcctggct
      181 gataggaaat atccatgttg tgtttgccct ggccagggaa tatggtggcc ttggcaatat
      241 acttggctgg caatattttg gaaagaaaga acctgagagg gaattacctc ccgtggtgct
      301 tatttctgca gtaagtctta tccccggcat actagccttt atcgccggtg caatcttcct
      361 gattatgtat ctgttgcaga attttgaacc gggattgact tttgacaatc ttttagtcaa
      421 gaatctgatt ttctttttcg ggcacacgct tgtcaatata acgctatatt gctgtcttgg
      481 atgggtctat accataatgc cggaatatac tcacagagaa tggaaagtca acaaactgtt
      541 ggtttgggca tggagtggga ctttcttttt tattttattt gcatatttcc accatttata
      601 tatggatttt gctcagccaa agggaatgca atatgcaggg caactcgctt cgtatttcag
      661 tgccatccct gccacggcag taactatgtt cggcatgatt gcccaggtgt atcatgccaa
      721 gttaaaatgg agcatagttc cactggcctt cctgatggga gtggcaggat gggcgattgg
      781 aggatttgcc gcaatcatag actctaccat tgcattgaac aaaatcctcc ataataccct
      841 ttgggtgccg gctcatttcc atacctacat gctaggagga gtggtacttt ttattttagg
      901 atacctattc tatatcacac acagtaagga ggaattgctc aaaaacaata tggccaaatt
      961 tgggttctgg tccttcctgc tggccgctca cggctttctg gcattcttct attccggagg
     1021 attgtatagc ataccaagga ggtactccga ctataaaggc ataccaatgg aagaaaccca
     1081 caatataggt gccaatatag cagccgtttc gaccatattt atcagttttg tattgattgg
     1141 attattgatt atgtatgttt ctttgtttaa gaaaatactg gcgaagaata cgcaggatgg
     1201 aataatctga cacctgcttc tgagtaattt ttcacagcat tatcctgtca gtagatgaga
     1261 aatgtattta ttccaattct ttctttaatc atttgtctga cagtaatagg agtctggacc
     1321 ctgggttttt catccttcac cattttctca tataccttga aaaaggcagg tgaattaccc
     1381 aggcctcttc cggatatcca gatgatcaat cacgaaggta ttgtttttaa tccgggtgaa
     1441 ataaaaaagt acaaactcat caactttgtt tacctcaact gtccttttgt ctgtcacaag
     1501 atcaacaacc gcctggagca aatttacaat aagattgata cgaatttagt tccgaatgag
     1561 atcgagttcc tgactgttag ttttgatctg aagaacgacg atgtaaagaa aatcaagaat
     1621 tatcacaaac tatttaaagg cgaaatatcc ggctggacct tcgccctacc ctatcaaagt
     1681 actgaccgat attttacgga cttccttagt aagttgggta tctggaaata taccgtacca
     1741 tctaccgggt tgatcaatca ctccatatac atcttcctcg tcaatcgaca aaatcaaata
     1801 gtaagggtct tcgatcccgc cagggaaagc gatgaaatga tcattcaagg aattacacag
     1861 tgcctgaaag aaaaataaag attgcgatat tgagcctctt aggtataatg atctggctcc
     1921 tgtcattcac tctcaacgaa tggctgagtg agactatgcc cagacatcaa ttgattcaat
     1981 taccatcaat gttcttcata ggtgttttaa tgggaattac ggcatcccat cacctaaagc
     2041 cggatttgcc atggagtatt gctgtattga tcttcgtaat gttctctctc ttgttctgga
     2101 tgattcccca ttcgatagat tatgctgtca taagccctgg attcaaccgt atcatgcatg
     2161 ccaacatgct tctggcaggc ttccttacca tttcttccat acgacacacc attccggaag
     2221 ttaaaatcat cttcctgggt atggtggcag ctatgatcat cgccggcggc attgctttgc
     2281 tgtccttcaa tattttactg tgtagtgcat ttacaatcga acaacaaaag cagaccggca
     2341 ccctgcttac cttattgggc ttagctctgt attttttcat ggccttcata ttattttcaa
     2401 gcctttcgaa gaaataatca gaggatcata ccaggaaaat ttaatggttt gaacaagaac
     2461 atgcttcatc attgcaaagc tttaaaacaa ttcaagcata tgaaaaatgc gcatgaggaa
     2521 tatccgaatt ggttcaaagg gcaaccgagc atttttataa agaacatttg ttgcctcaat
     2581 tacaggatcg ctaaatacca cccttattta ccattaggcc gtataaaata cactggtaga
     2641 gacaattcac gttttgaaac atattaccag tattaaaact gaaatcgccc tgaacacaaa
     2701 gaatttcgcc gaaagtattt ggaattattc taaataatgc aataaatata taacgatata
     2761 tttgtcttta atacaaaatg gttttacatt tgcagtcgaa atctaaatct tggggcagaa
     2821 aataataaat aaggttttta gagagttgag atttgagatt tacgatttga gatttacgat
     2881 ttgagattta agatttgaga tttaagattt gagatttaag atttacgatt taagatttac
     2941 gatttaagat ttacgattta agatttacga tttaaaaaca ataatttata aatatggaaa
     3001 ataaagatat ctggctcgag tctttaatta cggctactcc acaagaagga cgtgaactgg
     3061 caattaaaat ggccagaaag acaattgcgg ccatacagac tgatcctgaa atcagaaaaa
     3121 aactcagatc agactatgcc aatgatacta cgcaactgat tgcatcggcc aatgtcgtcg
     3181 ccatcgagtt tcaaaccata gctcaggcaa acaattactg gaaatagtaa gcaaccattg
     3241 tcaagacaat aataggaatc agtttatatt gacaaaatcg ttgaagccct tatgcattca
     3301 atcgatatat acctgccatg acttattaca agataaaatt gtcttatttt cgaattttcc
     3361 caaaatattt tgacaaatcc ggagatcatt cactttgaag tattacgatg gaaattactg
     3421 attttgcaaa agcacagggt cattggatct tagctaaaat gggaaagcgg gttttaagac
     3481 ccggaggtaa ggaattaaca aaaaaactgg tctctgagct tcatatccag cacgatgatg
     3541 atctggtaga gtttgccccc ggccttggtt ataccgccaa cctcgcttta catcatgcac
     3601 cccactccta tattggcgtt gatgcagacg aagacgctgt cagagaactt aaaaatcgga
     3661 tcaaaggcag gaacaccaaa ttccaaattg gaaatgcggc tgagacccac ttgccagatg
     3721 aatccaaaga taaagtgttt ggcgaagcaa tgctgactat gcatgccgat cacagaaaat
     3781 ccgatatcat caaggaagca agacggatac taaagaaagg tggactatac gccattcacg
     3841 agttggggct ggtagatgtg gatgatacac ttaaaagtga gattcaaaag gagctagctt
     3901 tagccattaa agtcaatgcc cgtcccctca cggaagaaga atggagagca ttgttggaaa
     3961 aggaaggttt caaggtcaat aaagtatata ccaatggaat gttcctactc gaatatagac
     4021 gtatcctgga ggatgaagga tttttcagga cactgaagat tggtttcaac atcattaccc
     4081 acccaaaagc attgaaaaga attctgggaa tgaaaaaaat tttcagaaag tatcaacgac
     4141 atatgaatgc aattgttatc atttcagaaa agatttaagt aaaaatatac aacactatga
     4201 atattactaa agaaagtatc atcggcgatc ttgtggctca tgattacaga actgcttctg
     4261 tatttaaaaa gaacaagatt gacttctgtt gcaatggcaa tcgaagtatc gaagaagcat
     4321 gtgataaaaa gaatattgaa agtaaaggga ttattgagca gctacaagca gccaccgctc
     4381 aggcagcatc ctcaggtatt gacttcaacg catggccatt ggacttactc gctgattaca
     4441 ttgaaaagac gcatcaccgc tatgttgaga ataagattca tgaaatcatg cccttcctcg
     4501 aaaaaattgt acgtgtccat ggtgacagac atccggaatt ggcggaaatc gcacaattgt
     4561 tcaaggaatc tgctgaggaa ttgagtgctc acatgaagaa ggaagaactc gtgttattcc
     4621 catacatccg tcaaatggtg ttggcccgac aaagaggtga gacagtaagt gcacatttcg
     4681 gcactattca gaacccaatc cggaatatga tgcatgaaca cgacactgag ggagagcgtt
     4741 tcagaaagat ttccgatctc agcaacaact atacaccgcc cgctgacgga tgtacaacat
     4801 ataaagttac attttctatg ctaaaagaat ttgaagatga cttacatttg cacatccatt
     4861 tggagaataa catccttttc cccaaatcga tagaattaga aatggctttt taaccattat
     4921 atcatattta ataaccaaac ttaattttaa atcaaatgaa aaaaattatt tttctttttg
     4981 ccgtgacact attttttgca tcatgcggtg gtaacagttc tgcgcctgct actgatgaaa
     5041 ctgcaacaga agctactgct gaagaaggag gtgaagaagc aactgctgac gaagcagatg
     5101 ctaatatcgc tgaagttacc atcaagggtg acgatgcaat gaaatatgat ctttctgaaa
     5161 tcaaggtcaa ggaaggtcag actgttcatt tgacacttgt acactcagga aaggcacctg
     5221 ttgcagctat gggtcacaat tgggttctga tgaagccggg tacagatgtg gagaaattcg
     5281 ctactgctgc catcaatgcc aaagacaacg actacattcc tgctgacatg gccgcagacg
     5341 ttattgctca cactaagacc atcggaggcg gtgagacaac agatgttgaa tttactgcac
     5401 ctgcaaaggg tacttatgac ttcctttgtt ctttccctgg tcatcatgct acgatgaagg
     5461 gtaagttcat cgttgagtaa ttggaaaacg ttatcaaatg ttggatcggg acattttcgt
     5521 atggatgatg ttaccgacat ataacgaaaa ctaaactaag aagcggtata tgataaaaat
     5581 ataccgcttc atttttttaa cccctgacga tgtggaaccg gtttgcgaca atttcagttt
     5641 caaaacattc tttgctttca ttaacacacc tgcttccaac cgaaataaag aaaaacatat
     5701 aatccaaccg gcttcagctg tattttgttt acttcaatgc ttcaatcaac aggttaagaa
     5761 tcctttcctc atcgacattc atttcaataa tgcctcgtgc agtgtaagac atacgaccag
     5821 tttcttcaga tacaataaaa gtctttgcat tactgacttc gcttattcct agagccgccc
     5881 tgtgccttag tccggcgctg ggtggaacat tgatattgtc tgaaacggga aggatgcaac
     5941 tggcttttag aatccgatcc ttttgaatta ccaaagcccc
//
LOCUS       DAOQQN010000189         5957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5764_length_5957_cov_4.864114, whole genome shotgun sequence.
ACCESSION   DAOQQN010000189 DAOQQN010000000
VERSION     DAOQQN010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5764_length_5957_cov_4.864114"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3285)
                     /locus_tag="PLG24_07960"
     CDS             complement(<1..3285)
                     /locus_tag="PLG24_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HMZ24863.1"
                     /translation="MLDSIIKFSIRNKLIIGMMTIVLVIWGIWSATKLPIDAVPDITN
                     NQVQIITVCPTLAGQEVEQLVTFPIEQAIANLPNLEETRSISRFGLSTITVVFREDVD
                     IYFARQLISERLKEAEEDIPKGIGRPELAPVSTGLGEVYQYILHPKKGSESKYTASDL
                     RTMQDWIVARQIYGTEGVAEVNSFGGALKQYEVAVDPNRLKAMGVSVSDIFNALEKNN
                     QNTGGAYIDKKPNAYFIRGIGLVTTLEDIRNIAVKDETGSVPIFIKDVADVRFGSAIR
                     YGAMTYNGEKDAVGGVIMMLKGANSNEVVNSIKAKIPGIQKSLPDDVVIEPFLDRTDL
                     VKRAISTVERNLLEGALIVIFVLVLFLGNLRAGLIVASAIPLSLLFALGMMNIFGVSA
                     NLMSLGAIDFGLIVDGAVIIVEAILHLMSLEKTKTVLNSEEMDLVVEASAEKMMNSAA
                     FGQIIILIVYLPILSLTGIEGKMFTPMAMTVSFAILGALILSVTYIPMMSSKFLSRKT
                     THRETLSDRMMKIFQKFYQPLLQRAFRFKYLVVSAAIGLFLISLLAFRTLGGEFIPQL
                     QEGDFAFHCILPQGSSLTQSIETSMHASRIIKQFDEVKMVVGKTGSAEVPTDPMPPEV
                     SDLIILLKPQEEWKEQKSYNKLAEEILEKLESIPGIFFEANQPIQMRFNELMTGIRQD
                     VAVKIFGENMDTLLTYAEQSARVIQQISGVTTPQIEKVSGLPEINVEYDRTRLANYGL
                     NVEDVNDILSTAFAGRVAGQVYEDERRFDLVVRLDTPFRKNIDDVNSLMVPIRGGAQI
                     PLSQIARVSYKLGPAQISRESGKRRIVVGFNVSGRDVESVVSDIQEKLEQQVHLPSGY
                     YFTYGGQFENLQQASKRLLVAVPISLLLIFILLYFTFHSLKQAVLIYTAIPMSAIGGV
                     FALLIRGMPFSISAGIGFIALFGVAVLNGIVLIGTFNQLEKDGIRDLYQRVMEGTRSR
                     LRPVLMTATVASLGFLPMAVSHGAGAEVQKPLATVVIGGLVTATILTLLVLPLLYIIF
                     NTKIKRRMKPDGIVAFVAMLLMSFSSQAQTLVIDNADQAVEWAIKNNPGLRAKNLETL
                     AAHSLIR"
     gene            3525..3746
                     /locus_tag="PLG24_07965"
     CDS             3525..3746
                     /locus_tag="PLG24_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24864.1"
                     /translation="MSFKKEGGINILTCLKGGQRTAATTVAKRTTKVRMVLVIMGAAR
                     NLRQLIAMGFRMMNVLTRHDRQCQEINAE"
     gene            complement(3963..4772)
                     /gene="dapF"
                     /locus_tag="PLG24_07970"
     CDS             complement(3963..4772)
                     /gene="dapF"
                     /locus_tag="PLG24_07970"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HMZ24865.1"
                     /translation="MKISFYKYQGAGNDFIMLDGFSTSYDSGLDHRVVAALCDRRFGV
                     GADGLIIINPLEELLDFEMLYYNADGKPGSMCGNGGRCAFQFARDLRIISDRSEFLAT
                     DGPHEAFLHDDGNISLKMSDVKQVERLDARTFVVDTGSPHFVRIVDDIDAVDVFREGR
                     AIRYSDAYKEKGINVNFIEILSDRIRIATYERGVEDETLACGTGVTASALVSMELNLL
                     NGKIRVEAKGGTLFVEAERNTDNGQYESVWLTGPAIKTFEGTIETDDIQRS"
     gene            complement(4883..>5957)
                     /locus_tag="PLG24_07975"
     CDS             complement(4883..>5957)
                     /locus_tag="PLG24_07975"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HMZ24866.1"
                     /translation="VYDRNSGDLLCLFASHNGLFASTPTNKIRFNVARSSDFGKTWEA
                     PKEFSSQIYQSGWYAAWVASGSAHQMESGRIVAAIGARKNSSNTISNFMIYSDDGGLT
                     WNTAGQASATGDEAKIVELDDGRLLMAIRTSVRRRVTHSSDGGNTWETPIPVAELVEP
                     GVNGDLIRYTSVRKGFDKSRLLFSIPNHPTQRRNLTIFISYDEGKTWPVKRVISPIEA
                     AYSALTTFDDGTIGIFYENGEYENYQLNFARFSLDWLSEGTDSWSPSSAVAALSDSEE
                     MLSVSPNPASSTVEIKYSALQGRESLIEISDVSGRLVKKIVLEKPGRSSIIWHPEVST
                     DGVCFVKLITGGKMVVRKVMIVK"
BASE COUNT         1676 a         1479 c         1149 g         1653 t
ORIGIN      
        1 tcttatcagg ctatgtgcag ccaatgtctc taaattttta gctcttaagc cgggattatt
       61 ctttatggcc cattctactg cctgatcagc attatcaatg accaaggtct gtgcctgtga
      121 tgaaaagctc attagcaaca ttgccacgaa agcgactatt ccgtcaggtt tcatcctccg
      181 tttaatttta gtattgaaaa tgatatataa caatggtagc accagcaatg tcagaatggt
      241 tgccgtcacc agcccaccta tcacaacagt tgcaagtggt ttttgtactt cagcgccagc
      301 cccatgagac acagccattg gaaggaatcc cagagaagcc accgttgccg tcatcaacac
      361 cggcctcaac ctcgatctgg taccttccat gaccctttga tacaagtccc ttatcccatc
      421 cttttccagt tggttgaagg tgcctataag cacaatacca ttaagcaccg caacgccaaa
      481 cagggcaata aatccaatcc ctgcactgat actgaatggc atgccgcgta ttaataatgc
      541 aaacactccg cctatggcac tcattggtat cgctgtatag atcaggactg cctgttttag
      601 ggaatgaaag gtaaaataaa gcagtataaa aatgagtaac aaggatatcg gtactgctac
      661 caacaacctt ttactcgctt gttgcagatt ttcaaactgt cctccataag taaaataata
      721 tccggaaggc agatgtactt gctgttccag cttttcctga atgtcagaca ccacactttc
      781 tacatctcga ccggaaacat taaaacctac tacaatcctt cgtttgccac tttcccgact
      841 gatttgtgca ggcccaagct tatagctgac ccgggcaatt tgagagagag ggatttgagc
      901 accacctctg ataggaacca ttaggctgtt gacatcatcg atattttttc tgaatggagt
      961 atccaatctc acgactaaat caaagcgcct ttcatcttca tagacctggc ctgcaaccct
     1021 tcccgcaaag gcagtactta gtatatcatt tacatcttcc acattcagac cataattggc
     1081 caatcttgtg cgatcatatt caacattaat ctccgggaga ccggacactt tctcgatttg
     1141 aggtgtcgta acacctgaaa tttgctgaat aacccttgcg gattgttcag cataggtcag
     1201 tagcgtatcc atgttttcac cgaaaatctt aaccgctaca tcctgcctga tacccgtcat
     1261 caactcattg aacctcatct ggatcggctg attggcttcg aaaaatattc ctggaatgct
     1321 ttccagtttt tccaggattt cttcggccaa tttgttgtac gacttttgtt ccttccattc
     1381 ctcttgcggc ttcaacagga taatcaggtc ggaaacctca ggcggcattg gatctgtagg
     1441 cacttccgcc gagcctgtct taccaactac catcttcact tcatcgaatt gcttaatgat
     1501 acgtgaggca tgcatggatg tttcgatact ttgggttaaa gaactcccct gaggcaggat
     1561 gcaatgaaat gcaaaatcgc cttcctgaag ttgcggaata aattccccac caagcgttct
     1621 gaacgccagg aggctgatta aaaacaatcc aatggcagct gaaacaacca aatatttaaa
     1681 cctaaatgcc ctttgaagca atggttgata aaatttctga aatatcttca tcatccggtc
     1741 cgaaagcgtt tccctatgtg tagtctttct tgaaagaaac ttggaactca tcatcggaat
     1801 gtaagtcacc gaaagaatga gggcaccgag gatggcaaag ctcacggtca tagccatagg
     1861 cgtaaacatc ttaccttcaa taccggtaag tgaaagtatc ggcagataga caataaggat
     1921 gataatctgc ccgaatgctg cagaattcat catcttctct gcacttgcct ccaccacaag
     1981 atccatttct tcgctattaa gtactgtctt tgtcttttcc aaagacatga gatgaagtat
     2041 ggcttccaca atgatgactg ctccatccac aatgagaccg aagtcaatgg ctcccagact
     2101 catcaggtta gcactgactc caaatatatt catcattccc aaagcaaata aaagggaaag
     2161 tggaattgcc gatgctacaa ttaaacccgc tcttaaatta cccaaaaaaa ggaccaggac
     2221 aaaaatcaca atcaaggcac cttcaagcag gtttctttcc actgtactta tagcacgctt
     2281 gaccaggtct gttcggtcca gaaaaggttc gatgacgaca tcatcaggca gagacttctg
     2341 tatgcccgga atcttggcct tgatactatt gacgacctcg ttggagttgg ctcccttgag
     2401 catcataatg acaccgccaa cggcatcctt ctcaccgttg taagtcatag caccatatcg
     2461 gatagcgcta ccgaagcgta catctgctac atctttaata aaaataggca cgcttccggt
     2521 ttcatctttc acggcaatat tccgaatatc ctccagtgta gtgaccagcc ctatgcctct
     2581 gataaagtat gcattcggct tcttgtcaat ataagcgcca ccggtattct gattgttttt
     2641 ctccagggcg ttaaaaatat ctgagacact cactcccatt gctttaagcc gattggggtc
     2701 cactgcaact tcatattgct tgagtgcgcc accgaaactg ttgacctctg ccactccttc
     2761 cgtaccgtat atttgccgtg ccacgatcca gtcctgcatg gtcctgagat cagaggcggt
     2821 atatttggat tcgcttcctt tctttggatg caggatatat tgatacacct cacccagacc
     2881 agtactgacc ggtgccagct cagggcgtcc aatacctttt ggaatgtcct cctcagcttc
     2941 cttaagcctt tcactgatca gttgtcgtgc aaaataaata tcgacatcct ctctaaatac
     3001 tactgtaatc gtagaaagac caaagcgcga gatgcttctg gtctcttcaa gattcggcag
     3061 attggcaatc gcctgttcaa ttggaaatgt caccaactgc tccacctctt gaccggcaag
     3121 ggtggggcat acagtgatga tctgcacctg attgttggtg atgtcgggta ccgcatcgat
     3181 aggcagtttc gtggcactcc atatgcccca gatcaccagg acaatggtca tcatgccaat
     3241 gataagcttg tttcttatac tgaatttaat aatgctgtcc aacatacatg aaactcattt
     3301 gaaaatgatt agatcggttg gtgggcaatc tgattcaaaa acatctgttg caattcctgc
     3361 gttttattgc cgaataagta catcggtatt catctcaaac cattatgatt gaagtgcagg
     3421 cagaacaggc cttatatttt tcaaccaatt ctaggaggtt gccaaatatt tgcattgaac
     3481 gaagaaacaa aggtgatatc ccttacaata aaatatttag aaaagtgagt ttcaaaaaag
     3541 aaggtgggat taatatactc acttgtctta aaggcggtca acgtaccgca gcaacaacag
     3601 ttgcaaaaag gactacaaaa gtcagaatgg tcctggtcat tatgggtgca gcaagaaatc
     3661 tccgtcaact catcgctatg ggattcagaa tgatgaatgt cctcacaagg catgatcgac
     3721 aatgccaaga aataaatgct gaataaaatt accaggattt tcatctcgca gcaaatttat
     3781 ataatattta tgtttgcatt cactgtaaat tgcagtttgt acggaatcta aattgtaatt
     3841 gtcagtgggt cgcatcactt gtattcattg tcggacatcc aaccgtctta atgctcaggc
     3901 tggtacggta tttaaatgat attcggatat ttataccggc aaattgaagg ggacggagaa
     3961 tgtcaacttc tttggatatc gtcggtctca atagttccct caaaagtttt tattgcaggg
     4021 ccggtcagcc agacggattc gtattgaccg ttgtctgtat ttcgttcggc ctcgacaaac
     4081 agcgtccctc ctttggcttc aaccctgatc tttccattca acaaattaag ttccatggac
     4141 accaaagcag atgcggtcac accggttccg caagcaaggg tttcatcctc aacacctctc
     4201 tcatatgtcg ctatcctaat cctgtccgac aatatttcaa tgaaattaac attgattcct
     4261 ttttccttat aggcatcgct atatcgaatt gctctgccct ccctgaatac atcgactgca
     4321 tcaatatcat ccacaatacg aacgaaatgc ggagaccccg tgtcgacaac aaaggtccgg
     4381 gcatcaagcc gctcgacctg ctttacatct gacatcttta atgaaatatt gccatcgtca
     4441 tgcaggaaag cttcatgggg cccgtcagta gcaaggaatt ccgacctgtc gctgattatt
     4501 ctaagatctc tggcaaattg gaaggcacac cttcctccat taccacacat ggaacccggc
     4561 ttgccatcgg cattatagta taacatttca aaatcaagga gctcctccaa tggattgatg
     4621 ataatcaaac catcagcgcc cacaccaaat ctgcggtcac acaacgcggc cacaactctg
     4681 tgatcgagac cgctatcata ggaggtacta aaaccatcca gcatgataaa atcattacct
     4741 gctccctgat acttataaaa agagattttc acctattatc aaaatttcca attattagga
     4801 ttgtggcaac acaattcact ttttgatacg cttaaaatca taatgggatg ccttcgatca
     4861 tttcatgata ttgcattggc catcatttta caatcatcac cttcctaact accatctttc
     4921 cgccagtaat cagtttaaca aaacaaacac cgtccgtact gacttcagga tgccagatga
     4981 tgctacttct gcccggtttt tccaaaacaa ttttcttaac aagtcgtcct gacacatccg
     5041 aaatttctat caaggattct cttccctgaa gtgccgagta tttgatctca acagtggaac
     5101 ttgccggatt gggagacacc gaaagcattt cctccgaatc ggacaacgcc gccacagcac
     5161 tggaaggaga ccaggagtcc gtgccttcac ttagccaatc aagactaaaa cgggcaaaat
     5221 ttaactggta gttttcgtat tctccatttt cgtagaaaat accgatggtg ccatcatcaa
     5281 aggtcgtaag tgcggaatat gctgcctcta tcggacttat gacccttttg accggccagg
     5341 ttttgccttc atcatagctt atgaagatcg tcagattacg acgctgcgtt ggatgattcg
     5401 gaattgagaa taacaaccga ctcttatcaa acccctttct aaccgaagta tatcgtatca
     5461 aatcaccatt gacaccaggt tctaccagtt ccgctacagg tattggcgtc tcccaggtgt
     5521 ttcctccatc agaagagtga gttacccttc tcctaacgga tgtcctaatg gccataagaa
     5581 ggcggccgtc atccaattcg acaatcttgg cctcatcgcc tgtagccgaa gcctgaccgg
     5641 cagtattcca tgtaagacca ccatcgtcac tgtatatcat aaaattgctg atggtgttgc
     5701 ttgaattttt tctggcacct atagcagcga caatccgccc actctccatt tgatgggcgc
     5761 ttcccgaagc gacccatgct gcataccaac ccgactggta tatctgacta gaaaattcct
     5821 ttggtgcttc ccaggtcttg ccaaaatcac tacttcttgc tacattaaat cgtatcttat
     5881 tggtcggagt ggaagcaaac aagccattgt gagaagcaaa aagacacaac aggtctccgc
     5941 tgttccggtc ataaact
//
LOCUS       DAOQQN010000190         5946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5775_length_5946_cov_4.787982, whole genome shotgun sequence.
ACCESSION   DAOQQN010000190 DAOQQN010000000
VERSION     DAOQQN010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5775_length_5946_cov_4.787982"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1252)
                     /locus_tag="PLG24_07980"
     CDS             complement(101..1252)
                     /locus_tag="PLG24_07980"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HMZ24867.1"
                     /translation="MIFQRRYILLFFTVIILVAVIYFLGDIVTYIIGAWVLALIGTPI
                     KKAILRIPGLSKWKYGNTIASTLTILFFLFLIGFGFFLIIPILSEQFSVFASIDYNKI
                     AATLQEPLSKVEIYLKKAGILQDSVSLLQEFSAEIERVLIPTKVSDFFNGIVSTVANI
                     GLAVLSILFIGFFFLKDQGLFDSGIRSFVPDKYEAAISKALDDIAMLLSRYFSGIIIQ
                     SCAIWLIISTSLSILGFSNAWLIGFFAGMVNVIPYLGPWIGAGFGVLVTINANINLDF
                     SQEILPMIYQLILVFGITHLIDNFLIYPLLFSNRVIAHPVEIFIVVILGAKIGGLIGM
                     IVAIPSYTIIRVLAGVFFSEFKVVQKITAGIRKATEDSPDQSPPTPKAE"
     gene            complement(1327..2058)
                     /gene="fabG"
                     /locus_tag="PLG24_07985"
     CDS             complement(1327..2058)
                     /gene="fabG"
                     /locus_tag="PLG24_07985"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HMZ24868.1"
                     /translation="MNKYALVTGGSRGIGRAICMRLAEEGYNIIATYKSNDDAANELL
                     MLLNQNGRDHHLLKYDVADPRQTEAVLNDWIASHPGAEIDILINNSGIRKDNLLIFMP
                     ENDWNEVINTNLNSFYYTTKIVSSGMVKRRKGRIINIVSLSGIKGMPGQTNYSASKGG
                     LIAATKALAQELAKRNITVNAVAPGFIKTDMTGDLDEAELVKLIPANRFGTPQEVAEL
                     VAFLAGDKSGYITGQVISINGGLYS"
     gene            complement(2068..3597)
                     /locus_tag="PLG24_07990"
     CDS             complement(2068..3597)
                     /locus_tag="PLG24_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HMZ24869.1"
                     /translation="MIVLGKEALSVSEFEAIVLNKESIGIDAAALRHCIDNHEFLIKF
                     SENKIIYGINTGFGPMAQYRVDDRQRKDLQLNLIRSHCTGSGNLLPDSLVRGILLSRL
                     NTLLLGYSGVHPSVIHLLADFINKEIYPNIREHGSVGASGDLVQLAQVALALIGEGRS
                     NYNGSIMDTSAAFAENNLTPIEVVAREGLAVINGTAAMTGIGLVNNIRSKQLVRLAVM
                     ASCLINEIVNSFDDHFAKVLNDVKKHQGQKEIARMMRENLDGSNCIRQRDSIYYNNIP
                     ENAVFTDKVQEYYSLRCVPQILGPVMDTINYSEKILMDEINSVNDNPVISHEEEYVFH
                     GGNFHGDYISLEMDKLKIAITRLTMLMERQLNFLLSDNLNQKLPPFVNLGKPGFNFGL
                     QGAQFTATSTTAENQTLSFPMYVHSISCNKDNQDIVSMGSNSALLARKVIENAFEVIS
                     IHLMTIVTAIEYLHIQDKLAPATKKFYLSIREFLPPVVEDRPLSDIIDKATRKLKDFD
                     F"
     gene            3790..4908
                     /locus_tag="PLG24_07995"
     CDS             3790..4908
                     /locus_tag="PLG24_07995"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ24870.1"
                     /translation="MNLKKAILIIFLVNFCLLLSGQSKFPMSGDFLYYQVDRSPDIKD
                     VPNKGKVKFWDYSFLQSPYLKTHKFLANSKKDFLPPKSNLTMSVGNEDKYFFQSAKTG
                     FFLAGYSLEDPFGLGYRANVAYTNPLKTNLSGLKMGAVATEVSETYINYGIDNIKDDF
                     LKKLPVLPDSIRLNVLITRRTRLDGSGRLFLPDITLNTCYRVTREETYDYTIDTKTGN
                     LRWIDATDIFRENINNLPGGKTQILFYNDKYTLPVLFLTLDTESQKISKAEYLVPAKV
                     ALLKNKPTGNPDITLYPNPAYTKEIKLEVTNIQPGRYTFKIYSLIGKLEKAIPYYINN
                     SKFDTIDISDFKPGIYIYALVDGSGKIMLSKRLTIISP"
     gene            complement(4976..5290)
                     /locus_tag="PLG24_08000"
     CDS             complement(4976..5290)
                     /locus_tag="PLG24_08000"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HMZ24871.1"
                     /translation="MRRLMFFIFLTLSSCTKQKAHLTEEDVKFADVMVDIYMANGAAN
                     QIKAGNKDSLRNALVYDILMHQGIDTNAFYQKLRTMEKNPERFKLLTDTIVKKLERLS
                     NN"
     gene            complement(5287..5775)
                     /locus_tag="PLG24_08005"
     CDS             complement(5287..5775)
                     /locus_tag="PLG24_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HMZ24872.1"
                     /translation="MKTIYLIRHAKSSWDIHTIEDHERPLNDRGRRDAERMAEYLKGL
                     DIKPQLALCSTAKRTKETFSYFTRHFDSLPLQLTKKLYLAPVSVLEEEIHSISNEYDN
                     VILFGHNNGVSDLVSIILEKTVDLPTCSFTQIEFPVNDWKEADLKQARIAAAIAPKDL
                     PE"
BASE COUNT         1853 a         1209 c         1105 g         1779 t
ORIGIN      
        1 atattgattt ttaacatctt atgaaatttg taaaatttaa gtgccgaagt caggagcagc
       61 cagatacaga tacatttata tcacccggtt atttgcactc ctattctgcc tttggggtcg
      121 gagggctctg atccggtgaa tcctcggtag cttttctgat tccggcggta attttttgaa
      181 caaccttaaa ttcactgaaa aagacgccgg caaggaccct gataatggta tacgatggga
      241 tggctacaat catgccaatt aaccctccaa tcttggctcc gagaattacc acaatgaata
      301 tttccacagg atgagctatt acccggttgg aaaacaacaa tgggtaaatc aggaaattat
      361 cgatcaaatg tgtaatcccg aatacaagga tcaattgata aatcatcggt aggatctcct
      421 ggctgaaatc cagattaata tttgcattaa tggtcaccag aacaccaaaa ccggcaccaa
      481 tccaagggcc cagataaggt atgacattga ccattccggc aaagaatcct atgagccagg
      541 cattggaaaa tcccaaaata ctcaaggaag tggatatgat aagccatata gcacagctct
      601 ggataatgat gccactaaag taccgcgaca gaagcatggc tatatcatct aatgcctttg
      661 aaatagcggc ttcgtactta tcgggcacaa aggatctaat accactgtca aacagaccct
      721 gatccttcag gaagaaaaat ccgataaata gaatagagag gacagcaagg ccaatattgg
      781 ctacagtact gacaattcca ttgaaaaaat cagaaacctt ggtaggaatc aagacacgct
      841 caatttcagc agaaaattcc tgtaatagtg aaacactgtc ctggagaata ccggcttttt
      901 tcagataaat ctcaactttc gacaagggct cttgaagggt ggctgctatt ttgttatagt
      961 cgattgaggc aaatacactg aattgctcgg ataatatggg gataatcagg aaaaaaccaa
     1021 atcctatcag gaacagaaaa aacaatatgg tcaatgtaga ggcaattgta ttgccatatt
     1081 tccacttact gaggccgggt attcttagta tcgctttttt aatcggtgtg ccaatcaagg
     1141 ccagtaccca tgcgccaatg atataggtaa cgatgtcgcc caagaaatag ataacagcaa
     1201 ccaaaatgat gactgtaaaa aataaaagta tatacctacg ttgaaaaatc attggttgca
     1261 ggaggattga taatctcctt aattatgcaa aggtaattat tgtattcaga ataaaccgaa
     1321 aggatattat gaatacaacc cgccgttaat ggaaatgacc tgaccggtaa tataacctga
     1381 tttatcacca gctaaaaatg caaccaactc agctacttcc tgaggtgtcc caaatcgatt
     1441 ggcaggaatc agcttgacta gttctgcttc atctaagtct ccggtcatat ctgtcttgat
     1501 gaagcccgga gcgactgcat tgacagtaat atttcttttg gccagttcct gtgcaagagc
     1561 tttggttgca gctataagac cacccttgga tgctgaataa ttggtctgcc cgggcattcc
     1621 tttgattcct gaaagagaaa caatattgat gatgcgcccc tttcttcttt ttaccattcc
     1681 cgatgagaca attttggtag tataataaaa agagttcaga ttggtattga tgacttcatt
     1741 ccagtcattt tccggcataa agatcagcaa gttgtccttt ctgatccctg agttattaat
     1801 cagtatgtct atttccgctc ccggatggct cgctatccaa tcattcaata cagcttctgt
     1861 ttgtcttgga tcggcaacat catacttcaa aagatgatgg tctctgccat tctgattaag
     1921 aagcatcaac aattcattgg ctgcatcatc attactcttg taggtggcaa tgatgttgta
     1981 tccttcttca gcaagtcgca tgcagattgc cctgcctata ccacggctgc cacctgtaac
     2041 cagtgcatat ttattcattt cggacattta aaaatcgaaa tcttttaatt ttctcgttgc
     2101 cttgtcgatg atatcagata aaggtctgtc ttcaacaaca ggaggaagaa actccctgat
     2161 acttaggtaa aacttctttg ttgccggagc taatttatct tggatatgaa gatattcaat
     2221 agccgtgacg atggtcatca aatggatgga tattacctca aaggcattct ctataacctt
     2281 tcgggctaat aaagccgaat tactacccat gcttacaata tcctggttat ccttattaca
     2341 agaaatacta tggacataca tcgggaatga cagggtttga ttctctgctg ttgtggaggt
     2401 tgctgtaaat tgtgctccct gaagaccgaa attgaatccc ggtttgccaa gattcacaaa
     2461 gggaggaagc ttttgattca agttatcact cagcaggaaa tttaactgac gttccatcaa
     2521 catagtcaat ctggtaatag caatctttaa cttgtccatt tccagagaaa tatagtcacc
     2581 atggaaattt cctccatgga agacatattc ctcctcatgt gagattaccg gattgtcatt
     2641 gacggagttt atttcgtcca tcaggatttt ttctgaataa tttatagtgt ccataacagg
     2701 accgaggatt tgaggcacac acctcaacga ataatattcc tgcactttat ctgtaaaaac
     2761 agcgttctcg ggtatgttat tgtaataaat actatcccgt tgtctgatgc aattgctacc
     2821 atcaagattc tcacgcatca tccgtgcaat ttctttctgg ccctgatgtt ttttgacatc
     2881 attcaatacc ttggcaaaat ggtcatcaaa tgaattgaca atttcattga taaggcaaga
     2941 agccatcaca gcaagcctaa caagttgttt agacctgata ttattgacca atcctatgcc
     3001 ggtcatagcg gcagttccat tgataacggc aaggccttca cgtgctacaa cctcaatcgg
     3061 agtaaggtta ttctctgcaa aggcagcact agtatccatt atacttccat tgtaattact
     3121 tcttccttcg ccaatcaaag ctaaagcgac ttgtgccagt tgaacaagat caccgcttgc
     3181 cccgacactt ccatgttccc ggatattggg atatatttcc ttgttgataa aatcagctaa
     3241 aagatggata acagaagggt gtacgccact gtaacccagt aaaagggtat tcaacctgga
     3301 caataatatc ccccggacca atgaatcagg caataagttc ccgcttccgg tacaatgaga
     3361 tctgatcaaa ttaagctgca aatccttcct ttgcctgtca tcaacgcggt attgagccat
     3421 ggggccaaaa ccggtattga tcccatatat tattttattt tcggaaaact ttattaaaaa
     3481 ttcatgatta tcaatgcaat gtctcaaagc agcagcatca atcccaatgc tttccttatt
     3541 gagaacaatc gcttcaaact ctgaaaccga caacgcttcc tttcctagta cgatcattat
     3601 aaatatttct tgggtattaa agaggcgcaa aactacaaaa aagtgataaa tgttaaaatt
     3661 gaaaaatatt cggttcaatg ataatgccag aaccaattgt gccgtttgta ttttatcttc
     3721 gaaattctaa agaagttttt agttggaaat atttagtttt gcaaggttta ttgctaacaa
     3781 acaacacggt tgaatttgaa gaaagccata ctcattattt tcctggttaa tttctgcctt
     3841 ttactatccg gtcagtctaa gttcccaatg tcgggcgatt tcctgtatta tcaggttgac
     3901 agatctcctg atataaagga tgttcctaat aaaggaaagg tgaaattctg ggattattcc
     3961 tttctacaat ccccttattt aaagactcat aagtttctgg ccaattccaa gaaagatttc
     4021 ctgcctccaa aatccaatct tacaatgtca gtaggaaatg aggataaata ttttttccaa
     4081 tcggccaaaa cgggcttttt tcttgcagga tatagtctgg aagatccttt cggacttgga
     4141 tatagggcta atgtcgctta taccaatcca ttaaaaacta atttaagcgg attaaaaatg
     4201 ggtgcagtcg caacggaagt ttctgagaca tacatcaatt atggcataga taatattaag
     4261 gatgactttt taaaaaaact tcccgtcctg cccgatagca tcagattgaa tgtcctaata
     4321 accagaagaa ccagacttga cggatccggc aggcttttct taccggatat cactttgaac
     4381 acttgttatc gggtgacccg ggaagaaaca tatgattaca ccatcgatac aaagacaggt
     4441 aatcttcgtt ggatagatgc cactgacata ttcagagaga atataaataa tttacccgga
     4501 ggcaagacac aaatactatt ttataacgac aaatatactt tgccggtttt gttcctgacc
     4561 ctggatacag aaagtcagaa aatttcaaaa gcagaatatt tggtacccgc aaaagtagct
     4621 ctgttgaaga acaagccgac cgggaatccc gatatcacac tgtatcccaa tcctgcctat
     4681 accaaagaga taaaacttga agttacaaat atccaaccgg gaagatatac ttttaagata
     4741 tattccttga tcgggaaact cgaaaaagct ataccatatt acatcaataa ttcaaaattc
     4801 gatacaattg acatttctga cttcaaaccc ggtatttata tctatgccct ggttgatggt
     4861 tccggcaaaa tcatgctatc caaacggctg accattatct ctccataatg aagatgatag
     4921 acaacaatgg ttaatataaa gctctgagta ttaatttact tgaaatcttt caaaatcaat
     4981 tgttactgag cctttcgagt ttttttacga tggtatctgt aagaagttta aatcgttccg
     5041 ggttcttttc catagttctt agtttttggt aaaaagcatt ggtatcgatt ccttgatgca
     5101 tcaatatatc atatacaaga gcattgcgaa gtgaatcctt gtttccggct tttatttgat
     5161 tggcggctcc gtttgccatg tatatatcga ccatgacatc agcaaatttt acatcttctt
     5221 cggttaaatg agccttttgc ttggtacagc tagacaaggt caggaaaatg aaaaacatca
     5281 accttctcat tccggcaagt ctttaggtgc tatagcagca gcaatcctgg cttgtttgag
     5341 atctgcttct ttccagtcat tgaccgggaa ttcaatttgg gtaaatgaac aggtagggag
     5401 gtcaactgtt ttctccaata tgatggaaac caggtcactt acaccattat tgtgtccaaa
     5461 aagaatcaca ttgtcatatt cattgctgat actatgtatt tcctcttcca gaacagaaac
     5521 aggggccagg tataacttct tagtcagttg taatggcagg ctgtcaaaat gtctggtaaa
     5581 atatgaaaat gtctccttgg tccttttcgc tgtactgcac aaagctagtt gaggcttaat
     5641 atccaaacct ttaaggtatt cagccattct ttctgcatcc cttcgaccac gatcattcaa
     5701 aggccgctca tgatcttcaa tggtatgaat atcccatgaa gattttgcat gcctgattaa
     5761 ataaattgtt ttcataatgc caaaattaag gataaatcac taacttgccc gatttaatta
     5821 gattttttaa tgtattgagt gaaattaaat actgttatga cccaggaatg ttacggtatt
     5881 aatacattgg ccaattttta aaatgtgaaa aaaagaatcc gaatcagaga ttttgaatcc
     5941 tatttc
//
LOCUS       DAOQQN010000191         5944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5776_length_5944_cov_6.792494, whole genome shotgun sequence.
ACCESSION   DAOQQN010000191 DAOQQN010000000
VERSION     DAOQQN010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5944
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5776_length_5944_cov_6.792494"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="PLG24_08010"
     CDS             complement(<1..1296)
                     /locus_tag="PLG24_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ24873.1"
                     /translation="MNLFLKLLRFSRPYHHYIPEYIIYVFFFTIFGLLNFVMIIPLLD
                     FLFQENPISEPSSFPAFSFSASYLQDLFKYYAWQFSAGENQKFKLLAYITLILLTVTS
                     LKNFFGFMSQKVLARMRVNLVRKIRNQLFEKLTNTSVGFYHNRQKGHLLSTLSNDVGE
                     IENTVVTSIQVLFRDPFAIILTFTALFIISRELTFFTLLFFPLSGIAINKISRGLRKK
                     SVESQSLLGSLMSYTDEMISGNRVIKVFNAEGFVQRQYRMLNDKYTRVAKRIATNKEL
                     ASPLSEVLGVGVIAVIIIYGGHLVLAGQGELTASMFLGYLGLYFSILNPAKNIGQAYA
                     NLQRGMVSGARVFEILEQKDTIQNRPGAVAIDGFKEGIRFEHVGFRYSGETVVLNDFC
                     LNIPKGKTIALVGESGAGKSTVADLIPRFYDVTNGRVLMD"
     gene            complement(1710..2198)
                     /locus_tag="PLG24_08015"
     CDS             complement(1710..2198)
                     /locus_tag="PLG24_08015"
                     /inference="COORDINATES: protein motif:HMM:NF025675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HMZ24874.1"
                     /translation="MELFKIIVDIKKEDIIALFKYHQKQKRVRTNLIYVGLIILFTFF
                     FKSVKVEIGEAVLLAAILVLVFAFLNPRLGRLRYLKQFEANKNLQQPLEYHIFADRYM
                     VKTSIAESEVQWSTRYNISQNDDYIFIFHTEEMTSMLPKRCMTDDQLTFFGELIKRFG
                     KA"
     gene            complement(2312..3910)
                     /gene="pckA"
                     /locus_tag="PLG24_08020"
     CDS             complement(2312..3910)
                     /gene="pckA"
                     /locus_tag="PLG24_08020"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HMZ24875.1"
                     /translation="MSTTKNTDPRSICKTTGIENPARVYYNATPEQLIEETILRGQGL
                     LSDKGALVIKTGEFTGRSPKDKFIVTDAKTENEVNWGGFNTRFDADKFEKLEKKMLRY
                     FDGKDVFIRDNYVCADENYRLNVRTITEYPWSNMFANNMFLRPSAADTSEPEWTILCA
                     PEFYADPAEDGTRQHNFSIVDFTRKKVLIGGSGYTGEIKKGIFSVLNFLLPVDKGVFP
                     MHCSANTGRQGDTAIFFGLSGTGKTTLSADPERNLIGDDEHGWTNEGVFNFEGGCYAK
                     TVHLTEENEPEIYHAIKHGAILENMKFVEGSRTPDFEDVSITENTRVSYPMHHIGNAL
                     PVSKGPHPANIFFLTCDAYGVLPPISRLTKGQAMYHFISGYTAKVAGTEAGVKEPQNT
                     FSACFGAPFLPLHPARYAAMLGEKMEKFSTNVWLVNTGWTGGPYGVGSRMKLKYTRAM
                     ISQAIKGKLNNVAYHKHEIFGLNIPVSCPDVPSEILDPRNTWSDKEAYDRTAKKLAKD
                     FNDNFSKFADLASQEIRDAAPKHD"
     gene            4161..5564
                     /locus_tag="PLG24_08025"
     CDS             4161..5564
                     /locus_tag="PLG24_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HMZ24876.1"
                     /translation="MKSKKTSVIVLLAILFLVSILHVVDNFFSANVDKGILSVSHLLL
                     SLSFIYFAIMRRNLTTWILISMVIGALIGHEFPVVGQNMRVLSQVFLKLIKTIIAPIL
                     FSTLVVGIAGHSNLRQVGRMGWKSIVYFEIVTTFALFIGLIAINISKAGVGIQMPPDI
                     HETLPEVQQQTWQDIILHIFPENIARSIYHAEVLPIVVFSVLFGIGLAMVDEKKKRPM
                     LEFCESLAETMFKFTNIIMYFAPVGVGAAIAYTVGHMGLGILVNLAQLLMTLYVALIL
                     FILLVLLPVALIAKVPIVNFLKAVLEPMSIAFATTSSESALPKAMQAMERLGVPRKIV
                     AFVIPTGYSFNLDGTTLYLSLASIFVAQAAGIHMDIPTQLFLVFTLMLTSKGVAGVPR
                     ASLVILLATAAQFNLPTWPILIILGIDELMDMARTATNVLGNCLASCVVARWEGEFEP
                     RDPTIPLESELRQPQSA"
     gene            complement(5648..5845)
                     /locus_tag="PLG24_08030"
     CDS             complement(5648..5845)
                     /locus_tag="PLG24_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24877.1"
                     /translation="MRYLNNSAESAEVLPDTCPDSYATRALLCPGSETIRGSGMPRLQ
                     SYRPMKLVYNECTFGIRKNTG"
BASE COUNT         1708 a         1252 c         1201 g         1783 t
ORIGIN      
        1 atccataagg acgcgaccgt ttgtcacatc atagaatctc gggataagat cagccaccgt
       61 tgatttcccg gccccgctct caccaaccag cgctatcgtc ttccctttgg gaatgttgag
      121 gcagaagtca ttcaatacca ccgtctctcc cgaatatctg aaaccaacgt gttcaaaccg
      181 gattccttcc ttaaatccat ctattgcaac ggctcccggc ctattttgga ttgtatcttt
      241 ctgttccaga atttcgaata ccctcgctcc ggagaccatt cctctttgaa gattggcata
      301 tgcctggcca atattcttag cggggttcag aatggagaaa tataacccca aataccccag
      361 aaacatggat gccgtcaatt ctccttgacc tgcaagtacc agatgtcctc cataaatgat
      421 gattactgca atcacaccta cccccaatac ttcagacaag ggagaagcca gctccttatt
      481 ggtggcgatc ctctttgcaa cacgtgtata cttgtcattg agcatcctgt attgtctttg
      541 tacaaatcct tcggcgttga agactttaat cacacggtta cctgaaatca tttcatcagt
      601 ataggacata aggctaccta acaaactctg gctctcaaca gatttctttc ttagccccct
      661 ggaaatttta ttaatggcta tccccgataa tggaaaaaac aaaagggtga agaaggtcag
      721 ttcgcgggaa atgataaata atgcggtgaa ggtcaaaatg attgcaaaag gatctctgaa
      781 caatacctga atggaagtga ccacggtgtt ctctatttct cccacatcat tgctaagggt
      841 cgataacaag tgcccctttt gtctattatg ataaaaaccc acggatgtat tggtcaactt
      901 ttcaaacaat tgattcctta ttttccggac caaattgacc ctcattctgg ctaaaacctt
      961 ttggctcata aacccaaaaa agtttttcag ggaagttacc gtcaataata tgagtgtgat
     1021 ataggcaagc agtttaaatt tttgattttc gccagcactg aactgccagg cataatattt
     1081 gaataaatcc tgaagatagg acgcactgaa tgaaaatgcc ggaaaagacg atggttcact
     1141 gataggattt tcctggaaca agaaatccag cagcgggata atcatgacaa aattcaacaa
     1201 gccgaatatc gtaaaaaaga aaacatagat aatgtactcc ggaatataat gatgatacgg
     1261 ccgggaaaat ctcaatagtt ttaaaaataa attcatccgc tatttattcc atcagaatag
     1321 taatattcag aatagtaggc gtaaaaatac taatttgtcg tcattcgtta catttcaaaa
     1381 acagaacggc aaaaaagacc tgccaaaaag gctgtacttc gggcttccag aggtcaaaat
     1441 acaaccgaaa tttgaagtgg ggtggtgaaa aacgaagtgg gtaggaaatt aatagacgga
     1501 aatatacacc agaactcaat tggtaaaaat ctgctaaaaa attccgtcac ttttaaatct
     1561 tttgttagag tatatagatt ctgaagtgtt gtggcttaca cattatcacc tattaggcat
     1621 ctacctactt cgtgataaaa ttcaattcta ataacatcct caaaaacaag cccttcacaa
     1681 aatttacaaa atacggcatc tatatacgct caagcttttc caaacctttt gattaattct
     1741 ccaaaaaaag tgagttggtc atcggtcatg cacctttttg gcagcatgga tgtcatttcc
     1801 tctgtatgaa aaataaagat ataatcatcg ttctggctga tattatacct ggtcgaccac
     1861 tgtacttcag actcagcgat agaagttttt accatatatc ggtctgcaaa aatatgatat
     1921 tcaagaggtt gctgaaggtt tttgtttgct tcaaactgtt tcaaataacg taatcggccc
     1981 agtcgagggt taagaaatgc gaatacaaga accaaaatgg cagccagcag cacggcctct
     2041 ccaatctcca cttttacaga tttaaagaaa aaggtaaaaa gaatgatcag ccccacgtat
     2101 atcaggttag tgcggactcg cttttgtttc tgatgatatt tgaaaagggc gatgatgtct
     2161 tctttcttga tatcaacaat gattttaaat aattccatcc atttatattt gaaaagattc
     2221 gtctacgatt atccgtagaa taattggcca tttaaataaa aaaacgccac agacaagttg
     2281 tggcgtttca agattatatt tcattaaagt atcagtcgtg ctttggtgct gcatctctaa
     2341 tctcctggct agccagatcc gcaaacttgg agaagttgtc gttgaagtct ttcgccaact
     2401 tcttagcggt tctgtcatat gcttccttat ctgaccatgt atttcgtgga tccaggatct
     2461 cagacggcac atccgggcaa gataccggaa tattgaggcc aaatatttca tgtttatgat
     2521 aagctacatt atttagtttg cctttgatag cctgagaaat catcgctcgg gtatatttta
     2581 atttcatcct actgccgaca ccatagggtc cgcctgtcca gcctgtattt accagccaaa
     2641 cattggtact gaatttttcc atcttttcac caagcatagc agcatatctt gccggatgaa
     2701 gcggaaggaa cggggctcca aagcaggcag aaaaagtgtt ttgaggctct ttgacgcccg
     2761 cttctgtccc tgcaaccttg gcggtatatc ctgaaataaa gtgatacata gcctgtcctt
     2821 ttgtcaatct tgaaatcgga ggaagcacac catacgcatc acaggtcaaa aagaaaatat
     2881 tggcaggatg ggggcctttt gaaaccggaa gtgcattgcc gatatgatgc ataggatagg
     2941 aaacacgtgt gttttcagta atcgaaacat cttcaaaatc aggagtgcgg ctgccttcta
     3001 caaatttcat gttttcaaga atggcgccat gtttgattgc atggtatatt tcgggctcat
     3061 tctcctcagt caggtgtact gtcttggcat aacatccacc ctcgaagtta aatactcctt
     3121 cattggtcca tccgtgttca tcgtcgccta tcagatttcg ttcagggtcg gcagagaggg
     3181 ttgtcttacc tgttccgcta agtccgaaga atattgccgt gtctccttgg cgaccggtat
     3241 tggcggagca atgcatcggg aatactccct tatcaaccgg cagaaggaaa ttcaagacag
     3301 aaaaaattcc ttttttgatt tctccggtat aacctgagcc ccctattaaa actttttttc
     3361 gtgtaaaatc aacaattgaa aaattatgct gtcgagtacc atcttctgcc ggatcagcat
     3421 aaaattcagg ggcacaaaga atcgtccatt ccggttctga agtgtctgct gctgaaggtc
     3481 tgagaaacat gttattggca aacatattgc tccaaggata ctcagtaatc gtacggacat
     3541 taagtctgta attctcatcc gcacaaacat aattgtctcg aatgaatacg tccttaccat
     3601 cgaaataccg caacatcttt ttctccagtt tttcgaattt atctgcatca aagcgagtgt
     3661 taaagccgcc ccaatttact tcattttccg ttttagcatc cgtgacaata aatttatctt
     3721 ttggcgatcg cccggtgaat tcgccggttt taatgaccaa cgcgccttta tcagaaagca
     3781 ggccctgacc tcgaaggatg gtttcttcaa tcaattgttc cggagtggca ttataataaa
     3841 ctcttgccgg attttcaatg ccggtagttt tacaaatgga tctgggatcg gtatttttgg
     3901 tagtgctcat agagagaatt ttaaatttaa agacgacaaa tttagaagaa aaggccgaag
     3961 gttcaaaata aattttggaa ttttgatgta attaagatta tttgttttga aaaaatagaa
     4021 ttacattgca tcatttttga atgaattttc cttttattaa aaaaatcaaa aggaatcgag
     4081 acgcgaacca caaaataatt aattttaacc gttttaatta aattgttggc aatttttttt
     4141 aagatctgaa aaacatacgg atgaagtcaa aaaaaacaag tgttatagta ctgttagcaa
     4201 ttctattttt agtcagcatc ttgcatgtgg tagataattt cttcagtgcc aatgtcgata
     4261 agggaatctt atctgtatcc catcttttat tgagcttaag ttttatctat tttgccatta
     4321 tgcgtcgcaa tctgacgacg tggatactga tatcgatggt catcggggcg ttgattgggc
     4381 atgaatttcc ggttgtgggt caaaacatgc gtgtccttag ccaagtattc ttgaaattga
     4441 taaagactat catagcacca attctgttct cgacgttggt tgtaggaatt gccgggcatt
     4501 ccaaccttcg tcaagttggt cggatggggt ggaagtctat cgtttacttt gagattgtta
     4561 ctacttttgc cctctttata ggattaatcg ctatcaacat atcaaaggcc ggagttggta
     4621 ttcaaatgcc cccagatatt cacgagacat tgccggaagt tcaacaacaa acctggcagg
     4681 atattattct gcatattttt cctgagaaca tagctcgttc tatttatcat gcagaagtct
     4741 taccaatcgt tgtctttagt gtgctttttg gaatcggtct ggctatggtg gatgagaaga
     4801 aaaagaggcc tatgctggaa ttttgtgaga gccttgccga aacaatgttc aagtttacta
     4861 atatcatcat gtatttcgcc ccagttgggg ttggtgcggc aatagcatat actgtcgggc
     4921 acatgggatt ggggatattg gtcaatttgg cgcaactttt aatgacactt tatgtagcat
     4981 taatcttgtt catcctgctt gtacttcttc ctgttgcatt gattgccaag gtgccgatag
     5041 tcaattttct taaggcggtc cttgaaccaa tgtcaattgc ctttgccacc acatcttcag
     5101 aatcggcact tcccaaggcc atgcaggcta tggagcgttt gggtgtacct agaaagattg
     5161 tagcttttgt gatacccacc ggctatagtt ttaatcttga tgggacgact ctttatttat
     5221 cccttgcgtc catttttgta gcgcaggcag ccgggattca catggatatc ccgacacaac
     5281 tgtttcttgt cttcacactg atgcttacca gtaagggtgt agccggagtg ccacgggcat
     5341 cactggttat tttactggcg actgccgccc aatttaatct gccaacatgg cccatactta
     5401 ttatcctcgg gattgatgag ttgatggata tggcacgtac agccaccaat gtgcttggta
     5461 actgcctcgc cagttgtgtc gtggcacgat gggaaggaga atttgagcca cgggatccga
     5521 caattccatt ggaatcggag cttaggcagc ctcagtccgc ctgaattatt tcccatgaaa
     5581 ggcaggacag cccaagtata tcacaagaaa aataagtgac aaaagcttta tagagatttt
     5641 agccttgtca acctgtattt tttctaatac cgaaggtaca ttcgttatag accaatttca
     5701 ttggtctata cgactgtaac ctcggcatcc cggaaccccg gattgtttca ctcccgggac
     5761 agagcagagc tctagtagca tagctgtccg gacaagtatc cggcaatact tcggcacttt
     5821 cagcagaatt gttcaaatat ctcatgctgc ctttttacaa gacatcggcc tatgcctgac
     5881 cattctggcg atgatgtcat ccaggatttc gcctttcatg cggtgcctgt tgtagcggta
     5941 gcag
//
LOCUS       DAOQQN010000192         5943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5780_length_5943_cov_6.181895, whole genome shotgun sequence.
ACCESSION   DAOQQN010000192 DAOQQN010000000
VERSION     DAOQQN010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5780_length_5943_cov_6.181895"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLG24_08035"
     CDS             <1..202
                     /locus_tag="PLG24_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24878.1"
                     /translation="NIIGVLYAVRSGSEVYSYAIKSSVLIEFIQSFNKDIVITRPNTI
                     KNQPLVSKVNTLKDYVALVKVW"
     gene            269..1750
                     /locus_tag="PLG24_08040"
     CDS             269..1750
                     /locus_tag="PLG24_08040"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HMZ24879.1"
                     /translation="MTQEEFFTRYSIDFDNGWLGEGSFGTVYKAYDNYLNIWKAVKIA
                     KVRIIDGKQFSLVSEFQAIKSLPLHPNIAYYENVYQFQMSNGLFDYAVMQYYKDGNLK
                     QFLQSRTLDYESKRQVVEGLLQGVIFLHENNIIHRDIKPGNVMIAIHPQGMIIPKITD
                     FGLSRIKDGDISFMTGSIGGGTLEYSSPEQLYGKPISFNTDLWSFGVIVFEVMTGMKP
                     FYDDMSGNTEAQRLKLYQNITTGNISEKINTIPTPYKEIIELCLEADVRKRVDKGNIL
                     LNYLRKYDKVGATTGDMDEETYVMTKAEREEYEHIRDSHDINDLRMFVGKFPNSIFYG
                     EISARISSIQGNKHNGNQHPQSGLHKSTDKREHSKDTLTPHQSNSMAHISLKDRINRL
                     LARYRRYSYRDFRTSNTKFLYLIPILFFSFIVGYWAYSHFSKVEIYSEKGKYGLQSQL
                     KGIIYEAVFDTIIPLENGHFRVIQFNKTFIVDGMGNSQVDDTK"
     gene            1955..2470
                     /locus_tag="PLG24_08045"
     CDS             1955..2470
                     /locus_tag="PLG24_08045"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ24880.1"
                     /translation="MSHSDNTTSDLSLETNRLLIRPIQETDAAFMLILMNTRGWLDYI
                     GDRHITSLLLAKKYIAGLLREGKSHYFVVSEKDSGKEVGVLSLVQRDYLEYVDLGFAF
                     LPQFHGKGMAYEAASSLMARVKDINQTILAVTTPDNTASQALIEKLNFSFLKTIFRHD
                     QPLLLYIHTTN"
     gene            complement(2782..5133)
                     /locus_tag="PLG24_08050"
     CDS             complement(2782..5133)
                     /locus_tag="PLG24_08050"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HMZ24881.1"
                     /translation="MKLIFTIFSMVSLMVLGYGQPIKKVFIEEGSGTWCTNCPRGDVY
                     VRELTKKYPGQFVFVTVHLSDPMEIKEYADAVPFTGIPAGWFDRSSIYTLLPFTDLDS
                     DMAAHLSKEAPAEVTVQTGWNADTREITIKVSAEMAGNLSGDFRLAAIVVEDGVTGTG
                     PQYDQVNIYSGGALGPMGGYENLPNPVPASRMVYNHVARHLAGGINGAENSLPADLNA
                     GQSYSYVYKYVLPEDYNEEYVKVAGLLIDANKGTVLNAGQSSYLNGSDNARPFFHSVP
                     QESAFVGLDYQYVIITHDPDFDSLTITQTGDLPEGISFKDEGNGTAQLYGKPTVTGTF
                     PITLKLSDGRHEIEQSFILEVSDAKEDWIQVGKPGFSTFEATSISLKINKEGTAYVLA
                     ADISGKNIVVYSKDKNTEPWKQLGGVIKGDVFHSSMTLSDQGFPVVFSDKKVVGWNGT
                     EWKAIGTDLPGSACIYPAIVTAGDGTLYLVYFDIDKSTLTFKFDGSNWQPLGTVDKKY
                     TVWNQFKTDFAGRPMLIYGTEGVNISYSKVAILDNDQWVVQGGGYIEPASQTYFYHDA
                     VASSTGNIYAGLTIGAAEQGLNIYQLKDDKWELIKANVSGGASNYCNLNVGLGGKLIA
                     TFRDGKNDNKVSAMMYDGIEWQYLGKPGFTNASSYISMAIDPEGNPWVAYQDFGANKR
                     LSVKKYINLVSSVKNPKNEEPAIWLFPNPTNGEFTLRYSAGSHYRIFDICGKTLIKGT
                     LHQAGTNQTIKEQTIDAGNLGSGIYFITVEQGGLSKTLKFIKL"
     gene            5503..>5943
                     /locus_tag="PLG24_08055"
     CDS             5503..>5943
                     /locus_tag="PLG24_08055"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HMZ24882.1"
                     /translation="MAVNILPLFKEQFESTLISKASELTGENFENSKSAINSIAVAFL
                     EKVTVKTSTQKGSLAVLSLIGQHCFDEMAALISNKDKEYDFNKIMNLGLILIKNIFAQ
                     KSGALIDWVSSDSGVKSSSAASIMSMVAPFIMCPIGQQVKSNGMN"
BASE COUNT         1740 a         1261 c         1156 g         1786 t
ORIGIN      
        1 aaatattata ggcgtactgt atgctgttcg gagcggttcg gaagtttatt cttatgccat
       61 aaaatcatcc gtcctgatcg aattcataca atcatttaac aaggatatcg tcatcacacg
      121 acccaatacg atcaagaatc agccattggt ttcgaaggtc aataccttga aagattatgt
      181 agcgttggtc aaggtttggt gatttgttga atttcgtcat ttaaatttat atcactattt
      241 ttacttcaaa aagtaaaaat aagcactctt gactcaggaa gaattcttca cgcgatattc
      301 gattgacttt gacaacggat ggttgggaga ggggtctttc ggtaccgtgt acaaggcata
      361 tgataattat ctcaacatat ggaaggcagt caagattgca aaggtgcgca tcattgacgg
      421 caagcagttt tcattggtgt ctgaatttca ggctattaag tccctcccgc ttcatcccaa
      481 tatagcatat tatgaaaatg tataccagtt tcagatgtcc aatgggctat tcgactatgc
      541 tgtcatgcag tattacaagg atggaaatct gaaacaattt cttcaaagca ggactcttga
      601 ctatgagagt aaaaggcagg ttgtagaagg cttgttgcag ggagttattt tcctgcacga
      661 aaacaacatc atccatagag atatcaaacc gggcaacgtc atgattgcca tacatcctca
      721 aggaatgatc atacccaaga tcacagattt cgggctcagc cgaatcaagg atggagacat
      781 ttcatttatg acaggaagca ttggcggggg gactctggaa tattcctctc ctgaacaatt
      841 atatggtaag cccatcagtt tcaatacaga tctttggtca ttcggcgtaa ttgtctttga
      901 agtcatgacc ggcatgaagc cattttatga tgatatgtcg ggcaatacag aggcgcagag
      961 attgaagctt tatcagaata taacaaccgg caatatctcc gaaaaaatca atactattcc
     1021 aacgccatac aaggaaatta ttgaactgtg ccttgaagct gacgtaagga aaagggtcga
     1081 taaaggaaac atcctgttga attatttgag gaaatatgat aaagtcggtg ccacaaccgg
     1141 tgatatggat gaggaaacct atgtgatgac caaagccgaa agagaggagt atgaacacat
     1201 ccgggacagc catgatatca atgacttgag aatgtttgtg ggaaaatttc cgaatagtat
     1261 attttatggc gaaatctctg caagaataag ttctatccag ggaaacaaac ataacgggaa
     1321 tcagcatcca caatccggat tacataaaag taccgacaaa agagaacatt caaaagacac
     1381 tttaaccccg catcaatcca attctatggc tcacatttct ctaaaggaca gaataaatcg
     1441 tcttcttgcc cgatatcgca ggtatagtta tcgcgatttt cgtacatcca atactaagtt
     1501 tctgtatctg attcccattt tattcttctc atttattgtc ggatactggg cctattccca
     1561 tttttccaaa gtggaaatat attcagaaaa aggcaagtat gggcttcaat cccagttaaa
     1621 agggattata tatgaagcgg tgtttgacac catcatccct ttggaaaatg gtcattttag
     1681 ggtaatacaa ttcaataaaa cattcatcgt tgacggaatg ggcaattctc aggtggatga
     1741 tacaaaatga tgtttttttt aataaaataa atttgctttc aattgcggtt ggtactctat
     1801 tacagaaaag acaatagact tagaatgaaa ttgcaaagtt ttttttactg ccaataatga
     1861 aagtatcctg cttggagaac acaatgattt ttaccgcccg ccaattgact tttttgtatt
     1921 taccagtttt gtaaatcaat ccattgactt tttaatgtca cactcagaca acacgacttc
     1981 tgacttatcc ctggaaacaa acagactgct gatcagaccc atacaggaga ccgacgccgc
     2041 gtttatgctt atcctgatga atacccgggg atggttagat tatatcggtg atcggcatat
     2101 cacttcgtta ttgctggcaa aaaaatacat tgccgggcta ctccgggagg gcaaaagcca
     2161 ttatttcgtg gtttcggaaa aagattccgg taaagaggtc ggtgtattat ccttggtaca
     2221 acgggattat ctggagtatg tagacctggg ttttgctttt cttccacagt ttcatggaaa
     2281 aggaatggca tatgaagcag cgtcctccct gatggccaga gtgaaggaca ttaatcaaac
     2341 tatccttgct gtgactacac ctgataatac ggcctctcag gcattaatag aaaaattgaa
     2401 tttttctttt ttgaaaacca tctttagaca cgatcaaccc ctccttttgt acatacatac
     2461 tacaaattaa tcgatggggt attgaacagt tgcctgcctg ctgaacaaat aagccggcaa
     2521 taacacggtt agtatcgcat gtcattttcc gacccttgtt ataccaagat gatttatata
     2581 atattccata aatttttggt atacttgtcc cgagagctaa gcgattcggg gatgccgaag
     2641 gtacagtcgg tattatatac ttttttccat tactccggag agtccttgcc ttccttttcc
     2701 aatttttggc aaaaggccat gtcggtctaa ttttttatta caaataaaat tgggccggtc
     2761 tcggcgcgcc ctcttttgtt cttataattt gatgaatttg agggtttttg ataatccgcc
     2821 ctgttctaca gtaataaaat aaatgccgct tcccaggttt ccggcatcta tcgtctgttc
     2881 ttttattgtt tgatttgttc cggcctgatg cagcgttcct tttatcaggg ttttaccaca
     2941 gatatcaaaa atccggtaat gacttcctgc ggaataacgc agggtgaact caccattggt
     3001 ggggttggga aacaaccaaa tggcaggctc ctcattttta gggttcttaa ctgaggatac
     3061 caggttgata tattttttga cacttaaacg cttattggca ccaaaatcct gatatgccac
     3121 ccagggattg ccttcgggat caatggccat ggatatatag gatgaagcat tggtaaatcc
     3181 gggtttgcca agatattgcc attctatacc atcatacatc atcgcactga ccttgttatc
     3241 atttttccca tccctaaatg tagctattag cttccctcct agaccgacat tcaggttgca
     3301 atagttggac gccccaccgc taacattcgc ttttatcaat tcccatttgt cgtctttcag
     3361 ttgatagatg ttaaggccct gttcagctgc ccctatagtc aatcccgcat atatgtttcc
     3421 ggtggatgag gcgacggcat catgataaaa atatgtttga gaagcgggct caatatagcc
     3481 gccaccctga actacccatt gatcgttgtc caaaatagcc acctttgaat aggaaatatt
     3541 tactccttcg gttccgtaaa taagcatcgg cctgccagca aagtctgttt tgaattgatt
     3601 ccatactgta tacttcttat caactgtacc caagggctgc cagtttgaac catcaaactt
     3661 aaaggttaac gtgcttttat cgatatcaaa atacacaagg tataaagttc cgtctccggc
     3721 agtcacgatg gcaggataaa tacaggcact gcccggaagg tctgtgccta tggctttcca
     3781 ttcagtacca ttccaaccta caactttttt atctgaaaaa acaaccggaa aaccctggtc
     3841 ggataaggtc atcgaactgt ggaacacatc tcctttgatc acgccaccca gttgtttcca
     3901 tggttctgtg tttttatcct ttgaataaac cacgatattt ttacccgaaa tgtctgcggc
     3961 cagtacataa gccgtccctt ctttatttat ttttaagctt atggaagtcg cctcaaaggt
     4021 ggaaaaaccc ggttttccca cctggatcca atcttcctta gcatcagaaa cttccaatat
     4081 aaaggactgt tctatttcgt gccttccatc cgataattta agggtaatag gaaaggttcc
     4141 ggttactgtc ggcttaccat acaattgagc ggttccatta ccctcatcct taaacgaaat
     4201 gccttccggt aaatctcccg tctgtgtgat ggtcaaagaa tcaaaatcgg ggtcgtgagt
     4261 tataattaca tattgataat ccaatcctac aaaagcgctt tcctggggta cagaatggaa
     4321 aaaggggcgt gcattatcac tgccattaag atatgagctt tgtcctgcat tcaacacggt
     4381 ccctttattg gcgtcaatca acaaaccagc cactttgaca tattcttcat tgtaatcttc
     4441 tggaagcaca tatttataaa catatgaata tgattgccct gcgttcagat cagcaggaag
     4501 ggaattctct gcgccattga tcccgccggc aagatggcgg gcaacgtggt tatataccat
     4561 tcgtgaagct ggaaccgggt tgggtagatt ttcatatccg cccatgggcc ctaaagctcc
     4621 gcctgaatag atatttacct ggtcatattg tggtcctgtt cctgtaactc catcttcaac
     4681 cacaatagcc gccaatcgaa aatcacccga gagattcccg gccatttcgg cactgacttt
     4741 aatagtaatc tcgcgggtat cagcattcca cccggtctgc actgttacct cagccggtgc
     4801 ttccttactg agatgcgctg ccatgtctga atccaaatcg gtaaagggca acaaagtata
     4861 aatggaagaa cggtcaaacc aacctgcagg aattcctgta aagggcaccg catcagcata
     4921 ctcctttatc tccatggggt ctgataaatg caccgtcaca aatacaaatt gacccggata
     4981 tttttttgtc aactcccgga catagacatc tccccggggg caattggtgc accaggtgcc
     5041 acttccctcc tcaataaaca ctttcttaat aggttgtccg tatcccaaaa ccattaagga
     5101 taccattgaa aatattgtaa aaattagttt catgatcatt ttggttacct gtaaataaaa
     5161 aaccaaatta cagcttttct atcaattgat tccgataagg taagtatgta tttagacgca
     5221 tttttatatc ctttttagtt gctcaaatca tcggcgtact ttattacccc gccagccttc
     5281 cgtatccttg ccaatttatt catgaatatt tgccgccatt tccagatact cacatttaat
     5341 tgaattttaa ttctttaatc cctgatcatt tgttattttt acaaattata aattatacaa
     5401 aaacatcttc aaacgagtga ctttttaatt tttttacatc taataagatg gtgaggcatg
     5461 tattatgtgc ttttacgact aatttttaat atgttataaa ccatggcagt caatattttg
     5521 ccacttttta aagagcagtt tgagtcaacc cttatatcaa aggcaagtga attaaccggg
     5581 gaaaattttg aaaattcaaa gtctgcaatc aactccattg ctgtcgcttt ccttgaaaaa
     5641 gtcacggtga aaacatcgac acaaaagggt tcgttagctg tgctgagcct gattggtcaa
     5701 cattgttttg atgagatggc cgccctaatc tcgaataaag acaaagaata cgattttaat
     5761 aaaatcatga atctgggact tattctaatc aaaaatatct tcgcacaaaa gtcaggagct
     5821 ttgattgatt gggtctcatc ggattccggg gttaaatcat catcggccgc ctccatcatg
     5881 agcatggtcg ctcccttcat catgtgtccg ataggacaac aagtaaaaag caatggcatg
     5941 aac
//
LOCUS       DAOQQN010000193         5908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5841_length_5908_cov_4.464377, whole genome shotgun sequence.
ACCESSION   DAOQQN010000193 DAOQQN010000000
VERSION     DAOQQN010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5841_length_5908_cov_4.464377"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /gene="gltX"
                     /locus_tag="PLG24_08060"
     CDS             <1..1284
                     /gene="gltX"
                     /locus_tag="PLG24_08060"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HMZ24883.1"
                     /translation="PYRQSERLDIYRKYAGELIESGKAYYAFDTPDALENARKNAESS
                     GGAFLYGSANRLNMHNSLSMSPEEVKQALDAGEHYVIRLKVEADEVIEFTDLIRGQVQ
                     FSSNELDDKVLMKGDGFPTYHLANIIDDFLMRITHVIRGEEWLSSTAHHILLYRAFGW
                     EDQRPAFSHLPLILKPSGNGKLSKRDGDKLGIPVFPLSWEHPDLGKFFGFREAGFLPE
                     AMLNFIALLGWSPGENIEMMNMGEMIDHFSIEKIGKSGSRFDFDKAQWFNQKYIMQSC
                     ADKLALLVEGRFREAGFHGEVAELERIIDLFKERVTNINDFVTISRFIFTEPESFDTA
                     PIIKKWNDAVAEVFLAIIDKIRTIQPFQDSVLEPEIKGLIEAQGVGLGSIFPALRVAL
                     AGTAKGPDLFTMMELMGRNLVERRLENGFEMFNNK"
     gene            1288..1482
                     /locus_tag="PLG24_08065"
     CDS             1288..1482
                     /locus_tag="PLG24_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24884.1"
                     /translation="MGRKKKDELSPRPAIDLDIHINEFGEIMHGFDIDKINEFLNQNT
                     KDWRIKAENENESPFFDEEE"
     gene            complement(1580..3043)
                     /locus_tag="PLG24_08070"
     CDS             complement(1580..3043)
                     /locus_tag="PLG24_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HMZ24885.1"
                     /translation="MEVYARCSNINWILIIILISCHPGYGQTGAPLPVYPIPSPQQLA
                     WHRMEMNAFIHISINTFTDQEWGYGNEPAMVFNPTAADPDQWAKALSENHFKGIILTA
                     KHHDGFCLWPDENTEYSIRNSPYKDGKGDLVKETADAAAKYGMKFGIYLSPWDRNRGD
                     YGSKSYIKYYRKQLKTLLTNYGPVFEMWFDGANGGDGYYGGKNTNVYVNKQRYYNWPK
                     TIKTAEKLQPDILFFSDAGPDIRWVGNEKGQAGITNWNTINNDTLYAGKEGINAILAS
                     GSPDGKKWIPAEVDVSIRPGWFYHDKENSKVKSPEQLFDIYLTSVGRGAVLLLNVPPD
                     KNGLISDADLIALKGFSRLLKQRLGKNLAKNATVTASNVRGGAMEYAPERMLDDDMDS
                     YWCTDDGVTSASFDISLDQNQMVKYVLVQEYIPLGQRVKSFEIEALDGDQWKVVGQGT
                     TIGYKRIIEIPETITSKLRIWIKDAKACPLISNVQVY"
     gene            complement(3053..4513)
                     /locus_tag="PLG24_08075"
     CDS             complement(3053..4513)
                     /locus_tag="PLG24_08075"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HMZ24886.1"
                     /translation="MKIKFAESCQRGFDSTVSIITDQKQIKKVLAKLEIKAKASIIKD
                     QLKENGEMITTIDPQKGKVVFLKIDENKCNGNSSKVIRQTLIKNRNLFGSKITFLLPD
                     GDSESDERWARNVVRACMLSTNYLGKYKFKPNGLPRPQDVVICTISGNVPDAGVLEEE
                     KTIANARLEMMDLINTPSNIKTPEFIADHVARNGKRNGYKVEVFSEKDIKKFNLAALQ
                     SVNNGSDHPPRFVICHYKPKNAGKVKTLGLVGKGVTFDTGGISIKSSTNMHFMKSDMS
                     GAALVLTFTDLCARLQLPVEVITAVPLTENMVDGSSTKPGDVVGSYIGKSIEVIDTDA
                     EGRIILADGLGYLTKNYKTDAVIDFATLTGSIIQTLGYQVAGLFSNNDELAGQLYQAG
                     QTTSEKCWRLPIWDHYAEDLKSDIADVKNFNGKPVAGAITAALFLKEFTQDHPNWAHM
                     DIAGVSFTDNEYGSMRNATGFGISLLLEFVQKNMID"
     gene            complement(5298..>5908)
                     /locus_tag="PLG24_08080"
     CDS             complement(5298..>5908)
                     /locus_tag="PLG24_08080"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ24887.1"
                     /translation="ISEGIRYVFSNKVILSAITLDLFAVLFGGATAILPAFASEILHV
                     GAVELGWLRAAPSVGSAILLFLLSIRPPESKSGIKLLLAVTGFGLCMIAFSLSTNLWL
                     SIFFLFASGAFDAVSVVIRSTILQIYTPDHMRGRVSAVNTMFVGSSNEIGAFESGTAA
                     RLLGTAPSVLIGGSITLLVVALVARFAPTLRKLELNTIKLRE"
BASE COUNT         1611 a         1207 c         1262 g         1828 t
ORIGIN      
        1 ccttacagac aaagtgaacg attggatatc tatagaaaat atgcgggtga gctgattgaa
       61 tcaggcaaag catattatgc ctttgatacg ccggatgcct tggaaaatgc ccgcaaaaat
      121 gctgaatcat caggtggtgc tttcctttac ggatccgcca acaggctcaa catgcacaac
      181 agtctttcca tgtctcctga agaggttaaa caggctttgg acgcaggtga gcattatgtt
      241 atccgcctga aggtggaggc tgatgaggtg atagaattta ccgatctgat tagaggtcag
      301 gtgcaatttt cttccaatga gctggatgac aaggttttga tgaaaggtga cggtttccct
      361 acatatcatc tggccaatat catcgatgat tttctgatga ggattaccca tgtcattcgt
      421 ggtgaggaat ggctttcaag cacggcacac catatcctgc tatatcgtgc ctttggatgg
      481 gaggatcaga ggcctgcctt ttctcatctc ccactcatcc tgaagccttc tggcaatggc
      541 aaactgagta agcgggatgg cgataagctg ggaataccag tatttccatt gtcgtgggag
      601 catcccgact taggtaagtt ctttggattc agggaagcag gttttttgcc ggaagcaatg
      661 ctcaatttca ttgctttatt gggttggagt cccggagaga atatcgaaat gatgaatatg
      721 ggtgaaatga tcgatcattt ttccattgaa aagatcggaa agtcgggaag tcgctttgat
      781 tttgataaag ctcaatggtt caaccaaaag tacattatgc aatcctgcgc cgacaaattg
      841 gctttgctgg tggaaggcag gtttagagag gccggattcc atggagaggt ggctgagctg
      901 gagcgcatta tcgatctgtt caaggagcgt gtcactaaca tcaacgattt tgtaactata
      961 tcccgtttta ttttcacgga acctgaatca ttcgatactg ctccaattat aaagaaatgg
     1021 aatgatgcgg tagcggaagt ttttcttgcc attattgata aaataaggac tatacagcca
     1081 tttcaggatt cagtgctgga acccgagatt aaagggctca tagaggctca gggagtaggg
     1141 ttgggttcga tatttcctgc tttaagagtg gctttggcag gtactgcaaa ggggccggac
     1201 ctttttacca tgatggagtt gatgggtcgt aatttggttg aaagacgcct tgaaaatgga
     1261 ttcgaaatgt ttaataataa ataggcaatg ggaagaaaga agaaagatga attatctccc
     1321 cgcccagcca tcgatctgga tattcacatc aatgagttcg gtgagattat gcatgggttc
     1381 gatatcgaca aaatcaatga attcctcaat caaaacacca aggattggcg aatcaaggca
     1441 gaaaatgaaa atgaatctcc gttttttgat gaagaggaat agcaattctg tttctatagc
     1501 tgtcgtatag tccggttgca gccctgattt tagtggcagt aactaactaa tatggagtca
     1561 gatttgattt tagccgaaat taatatacct gtacattaga tatcaacggg caggctttag
     1621 catccttaat ccatattctc aatttggatg tgattgtttc gggtatttca atgattcttt
     1681 tatatccaat tgttgtaccc tggccgacaa ctttccattg gtctccatca agtgcttcta
     1741 tttcaaagga cttcaccctt tgtccaagcg ggatatattc ctggaccagg acatatttga
     1801 ccatttgatt ttgatcaaga ctgatgtcaa atgaggctga tgttacacca tcgtccgtac
     1861 accaatagga atccatatcg tcatccagca ttctctccgg tgcgtattcc atagctcctc
     1921 ctctgacatt ggaggcagtt acagtagcgt tttttgccag attttttccc agtctttgtt
     1981 ttagtaatct ggaaaagcct ttcagggcta tcagatctgc atcactgatt agcccattct
     2041 tatcgggtgg aacattgagc aataggacag ctcctcttcc tacagaagtc aggtaaatat
     2101 cgaatagttg ttccgggctt ttgactttcg aattttcctt gtcatgataa aaccatcccg
     2161 gccgtatgga aacatctact tctgcgggaa tccatttttt accatcaggt gatccggagg
     2221 ctaatatggc gttgatgcct tctttccctg cgtagagggt atcattattg attgtattcc
     2281 aattggtgat tcctgcctgt cctttttcat tgcctaccca tctgatatca gggcctgcat
     2341 cgctaaaaaa aagtatatcc ggctgaagtt tttcagcggt tttgatggtc ttgggccaat
     2401 tgtaatacct ttgcttattt acatatacat tggtattctt gcctccgtaa tagccatccc
     2461 caccattggc tccgtcaaac cacatctcaa agaccgggcc ataattggtg agtagtgttt
     2521 tcagttgttt ccgataatat ttaatatagc ttttactgcc gtaatctcct ctgtttcgat
     2581 cccatgggga taggtaaata ccgaatttca tgccatactt tgcagcagca tcggctgtct
     2641 ctttgaccag gtcacccttt ccgtccttat aggggctatt cctgatgctg tattcggtat
     2701 tttcatccgg ccaaaggcag aacccgtcat gatgcttggc tgtaagtatg attcctttga
     2761 aatgattttc gctaagtgct tttgcccatt ggtccggatc ggcagccgta gggttgaata
     2821 ccattgccgg ttcgtttcca tatccccatt cttgatcggt gaacgtattg atggagatat
     2881 gtatgaaggc attcatctcc atacgatgcc atgccagttg ctgaggagag gggatcgggt
     2941 agacaggcag cggggcgcct gtttggccat atcccggatg acaggatatt aaaataatga
     3001 ttagaatcca gtttatgttg gagcaccttg cgtaaacttc cataggaaaa tgttaatcta
     3061 tcatattttt ttgaacgaat tcaagcaaga ggcttatgcc gaaacctgta gcattcctca
     3121 tagatccgta ttcattatct gtaaacgaaa ctccggcgat atccatatgc gcccagttgg
     3181 gatgatcctg agtgaattct ttcagaaaaa gagcagcagt gatggctcct gcaacaggct
     3241 ttccgttgaa attctttaca tcggcaatat ccgacttcaa atcttcggca taatggtccc
     3301 agatcggcag tcgccagcat ttttcacttg tggtctgacc tgcctgatac agctgacctg
     3361 cgagctcgtc gttattggaa aaaagccctg caacctgata tcctaatgtc tgtataatcg
     3421 acccggttaa tgttgcaaaa tcgatgactg catccgtctt gtaatttttg gtcagataac
     3481 ccaatccatc tgccagaata atcctccctt ctgcatcggt atctatgact tctatgcttt
     3541 tgccaatata actgcctact acatcacccg gtttggtcga actgccgtcc accatgtttt
     3601 ctgtcaaagg aactgccgtt atcacttcga caggcagttg gagccgggca catagatcgg
     3661 taaatgtaag taccaaagct gctcccgaca tatcactctt cataaaatgc atattggttg
     3721 aactcttgat gcttatgccg ccggtatcga aagttacgcc tttgccgacg agcccaaggg
     3781 tctttacctt acctgcattt ttgggtttat aatggcaaat gacgaatctg ggcggatgat
     3841 cactgccatt gtttactgat tgaagtgctg caagattaaa ctttttaata tctttttcag
     3901 agaagacttc cactttgtag ccattccttt taccgtttcg ggcgacatgg tccgcaatga
     3961 attcgggagt cttaatgttg gaaggtgtat taataaggtc catcatctcc aaccgtgcat
     4021 tggctatggt tttttcctct tccaggactc ccgcatcagg cacattgcct gaaatagtgc
     4081 aaatcacaac atcttgtggc cggggtaatc cattaggttt aaatttatac tttccaagat
     4141 aattggttga cagcatgcac gcccttacca catttcgggc ccatcgttca tcggattccg
     4201 aatcgccatc cggtaatagg aaagtgattt tcgaaccgaa cagattcctg tttttgataa
     4261 gtgtctgcct gattacttta ctgctattgc cattgcattt gttttcatct attttaagga
     4321 agacgacttt acctttttgt gggtcaatgg tggttatcat ctccccattt tccttcaatt
     4381 ggtccttgat aatggaagct tttgctttaa tctccaattt ggccaagacc tttttgattt
     4441 gtttttgatc agtgatgatt gaaactgtag agtcaaatcc tctttggcaa gattctgcaa
     4501 atttaatttt catctaattt ttctttgttt taaaatcctg ataattctga ctttaacaga
     4561 ttgactatta gggtaatgga tttttatatc cggtctgtaa atgatgatct aaacatttaa
     4621 ccgaaaggat tccggccctt ataccgaaag aaaattttgt attcagcctg tttaatacat
     4681 ttttaacctt aataatggtg caaattatgg aaaaaatatc agttgagata aaatatattc
     4741 ctcaatgtga gctgaataaa atgaatcctt tcttctgccg ggaagaacag gtggattata
     4801 aaagatttta cgggttatga aaaagtgata atgtatagat aatatttatt ttatatatta
     4861 atataaatta aaatatatta tggaataggt caattaatta aagttgaata gtatacttaa
     4921 tggggtaata aaaacttagg aaacttcacg ctgctcttta aaactttgct attagcagct
     4981 ttgtcattag tcagattcaa tgtccggtct ttcttgaatc aaacaagtgt taagccggca
     5041 aaccgcccta gagcatcagg ttattatcaa tttaatacgc tgtttttttg attaggcacc
     5101 gattaatctt ttcctaagga acaattgata tggggcaatc tttcattttg cttattttcc
     5161 aattaatacc aaaaatttgt gggcaattat acaaatcatc ttggtataat ttgtaagtgc
     5221 aatattggac aggtctacac tgaactagtt cttatcagat gctttatcga tttggagcta
     5281 ttcgttgtaa gcacgcctta ttctcgcaac ttgattgtat taagctccaa ttttctgagc
     5341 gttggagcga acctggcaac cagggccacc acgagcaatg taatggatcc accaataaga
     5401 acagaaggtg ctgttccaag taaccttgct gcagtacctg attcaaaggc accgatttca
     5461 ttggaagatc caacaaacat agtattgact gcactaacac ggccacgcat atggtcaggg
     5521 gtatatatct gaagaatggt gcttcggatc actacactga cagcatcgaa ggctccactg
     5581 gcaaatagaa aaaatatgga taaccaaaga ttggttgaga ggctgaaggc aatcatgcac
     5641 aatccaaatc cggttactgc caacagtagt ttgattccgg atttactttc aggaggcctg
     5701 attgacagaa gaaataacaa aatagcggat ccgacggaag gtgcagctct caaccaccca
     5761 agctcgacag ctcctacatg tagaatttca ctggcaaagg ctggaaggat tgccgttgca
     5821 ccaccaaaaa gaacagcaaa taagtccagg gtgatggctg aaagtatgac tttgttactg
     5881 aatacatatc tgattccctc gctgattc
//
LOCUS       DAOQQN010000194         5878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5902_length_5878_cov_5.132749, whole genome shotgun sequence.
ACCESSION   DAOQQN010000194 DAOQQN010000000
VERSION     DAOQQN010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5902_length_5878_cov_5.132749"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLG24_08085"
     CDS             <1..207
                     /locus_tag="PLG24_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ24888.1"
                     /translation="IGRKKAVAEVFGVKFGGFFAWLTWLFVHLMSILGTKNKLMIFIN
                     WTVAYFTRDQSLRLIIKAKENKSN"
     gene            complement(223..2166)
                     /locus_tag="PLG24_08090"
     CDS             complement(223..2166)
                     /locus_tag="PLG24_08090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ24889.1"
                     /translation="MQLSIIIVNYNVRFFLEQCLSSIQKAGRTLDMEVFVVDNNSPDD
                     SVKMVRERFPMVNLIENKENFGFSAANNQAILQAKGKYILLLNPDTILSEDTLRICLD
                     FMETHDDAGAVCVKMIDGSGQYLPESKRGKPTLSASFFKMTGIYKLFPKSGFFNAYYS
                     GDISPNQIASIEVMTGAFMFLRQEVVRMIGLLDDTFFMYGEDIDYSYRILEAGYKNYY
                     LPTTTIIHFKGESTRKSTFNYTRTFYNAMIIFVKKHYKKQGRFYILFLQAAVIFKAIS
                     SYFLERILKILPLLIDFGLVYKALIGLKKWWGEWYFSNPDHINRQFDIVNAPLYAGIW
                     VLSLLAAGYYRARGAWTELLKGITGGTGVILIIYALLGQALRNSRSIIIWGALTTTLI
                     VIITKWLLNLLKYKRFGFKAERQIKYAIISGHSESERIRSLMSKNNRTYLFAGRICPD
                     DSIEEGTIGNLKHIEEIVDAYALSEVVFSQKDVAANKMMESMAQLTNEVDFRVAPDDT
                     LSIISSGDKNKQGELLTVGLNLNICTGGNKLKKRILDFTIAILLFMISPVIVLFQKKK
                     DNFFKNIFSVFYGQKTWVGYIEPIDSRILPTIKPGVLPCSGELTENSDADTVEKENLY
                     YARNYTWWKDLEVILKNLSNLSE"
     gene            complement(2246..3583)
                     /locus_tag="PLG24_08095"
     CDS             complement(2246..3583)
                     /locus_tag="PLG24_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HMZ24890.1"
                     /translation="MEQLKGKQSVGAGFVALGLIFMFSGLIFGTIGAIQYVIPGLFKP
                     GLGFDKVRPLHVSSVVFWILMGACGSVLHYISESQGRLWSERLAKWQRFLFATSMIAI
                     LVSYVMGMFGGREYWEFNPLLAIPIIAAWLLFIINVFKSIKSFRHQPVYVWMWLTGAA
                     FFLFTYIESYLWLIPAFRSGIIHDMTIQWKSYGSMVGGWNMLIYGTSIFLMDKISGTQ
                     RYSYSGIAFSIYFLGLFNLMFNWGHHIYTLPGTKIIQYISYLVSMTELLLLGRIIYYW
                     RSSVSTAMRHYHILPYRFMFAADIWIFLTLILAIAMSIPAINAYTHGTHITVGHTMGA
                     TIGINTFLLLAFAADIFDMHIAKPSRTISISLYIAVGALFIFWISLVVAGIHKAYWQM
                     HTPEEAYSIMMYSLRPYFIIFMISGIILASALTVIIVSLLKRYFKMVFDFLLK"
     gene            complement(3564..3962)
                     /locus_tag="PLG24_08100"
     CDS             complement(3564..3962)
                     /locus_tag="PLG24_08100"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ24891.1"
                     /translation="MADKIKVGILAVLSILFFCYTAYLYNRNYDPNAHSGLMSDKGKQ
                     IWQDKNCTACHQIYGLGGYLGPDLTNVYSHRPKDFIISRIQYGTDIMPAYQLSETQIE
                     EILAYLKSLDESGIASPAKLKLNIDGTIER"
     gene            4376..>5878
                     /locus_tag="PLG24_08105"
     CDS             4376..>5878
                     /locus_tag="PLG24_08105"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF542 domain-containing protein"
                     /protein_id="HMZ24892.1"
                     /translation="MSATNETVINVQEIEPRLRHLTIFQAFDSLEEGESLVIHNNHDP
                     KPVYYQLARLRGEVFDWEYLEAGPEWWDVKVTKRPDADTYFENQTYEEIVIDVPSLEP
                     RVKHATIFQTFNQLRPGQSFIIHNNHDPRPVYYQLMDLRGNIFLWEYLQQGPEWWDIR
                     VTRTVPMIPMEEGRMIINVPAIDPVHKHGTIFHVFENMNPGESFIIHNDHDPKPLFYQ
                     LKDRHGDSFTWDYIMQGPHWFDILITIKEPSITADKEEVIIDIPSIEPAKKHATIFRM
                     FEELEEGKSFIIHNNHDPKPVYYQMLEMFGDIFDWEYLRQGPQWWDIRVTRRKSVTQP
                     NQRQPIDNDDAIIVDVPSLEPRLKHVTIFETFDKLEPGESLIIHNDHDPKPVYYQLQA
                     ERGDVFTWEYLQQGPQWWDIRVTRKGTETNETIGEMVAKDLRKADIFKKYGIDFCCNG
                     KKTVRQACADKGIDPVVVERELQQPTQESAYGHQLNYNEWTPDFLADYIIN"
BASE COUNT         1801 a         1141 c         1187 g         1749 t
ORIGIN      
        1 atcggacgga aaaaggcggt agctgaggtc tttggggtca aattcggcgg attctttgcc
       61 tggctgactt ggctttttgt ccacctgatg tcaattctcg gcactaagaa taagcttatg
      121 atctttataa actggacagt tgcatatttt acaagagatc aatctctgag acttattata
      181 aaagccaagg aaaataaatc gaattgaggc agtcggaatt aattattctg acaaattgga
      241 caagtttttc aggattactt cgagatcttt ccaccaggta taatttcttg catagtaaag
      301 gttttccttt tcaacagtgt ctgcgtcaga attttctgtt aattcaccgg aacagggtag
      361 gacaccaggc ttgatcgtcg gtagaattct ggaatcaatt ggctctatat atccgaccca
      421 tgttttttgc ccataaaaca ctgagaatat gttcttaaag aaattgtctt ttttcttttg
      481 aaataaaact ataaccggac taatcataaa aagcaatata gctattgtga agtccaaaat
      541 cctctttttc aatttgttgc ctcctgtgca gatgtttaag ttaagaccaa ctgtaagtaa
      601 ctctccttgc ttgtttttgt ctccactact gatgatgcta agtgtatcat cgggtgcgac
      661 cctgaaatcc acctcattgg tcagttgagc catactttcc atcatcttat tagccgctac
      721 atccttttgt gagaaaacga cttcactgag tgcataagca tccactattt cttcaatatg
      781 cttcaggttg ccgattgtgc cttcttctat ggagtcgtca gggcaaatcc ttcctgcgaa
      841 aagataagtt cggttatttt tggacatcag ggaacgaatt ctttctgact cagagtggcc
      901 tgaaataatg gcatatttaa tctgcctttc tgccttaaaa ccgaatcttt tatatttcaa
      961 aaggttcaaa agccatttgg tgattatgac tatcaaagta gtcgtcaatg ccccccagat
     1021 gatgatagac ctggagtttc gcagcgcctg acccagcagc gcataaataa taagaattac
     1081 acctgtgcct cctgtgatac ctttcaagag ctccgtccag gcacctctag cccgataata
     1141 accggctgca agaaggctca atacccaaat gccggcgtac aacggcgcgt ttacaatatc
     1201 aaattggcga tttatatgat ccggattgga aaaataccat tcaccccacc attttttcag
     1261 tccgatcaat gctttatata ccaatccgaa atctatgagc aatggcagaa ttttgagaat
     1321 tcgctctaga aaatacgatg aaattgcttt gaatatgaca gcagcctgaa ggaataatat
     1381 ataaaaccta ccttgtttct tgtaatgctt tttgacgaag atgatcatcg cattgtagaa
     1441 agtgcgggtg tagttaaacg tggatttcct ggtggattca cccttgaaat gaatgatggt
     1501 cgtggtgggt aaataataat ttttataacc ggcttccaaa atcctgtatg aatagtcgat
     1561 atcttcgcca tacataaaga atgtatcgtc taacaaacct atcattctta ctacttcttg
     1621 ccttaaaaac ataaaggctc ctgtcatcac ttcaatggag gcaatttgat ttggtgagat
     1681 gtctcctgaa tagtatgcat tgaaaaatcc gctttttgga aaaagcttat aaataccggt
     1741 cattttaaag aaggatgctg atagggtcgg tttgcctctt tttgattcgg ggagatactg
     1801 tccacttcca tcgatcattt taacgcatac tgctccggca tcatcatgag tttccatgaa
     1861 gtcgagacaa atcctgagtg tatcttcaga aagaatggtg tcagggttga gcaaaaggat
     1921 gtattttcct tttgcttgga ggattgcctg attgttagca gcggagaaac cgaaattttc
     1981 tttgttctca atgagattta ccattgggaa tcgctctctt accattttga cagaatcgtc
     2041 cggagagttg ttgtccacta caaaaacctc catgtccaga gtacgtcctg ccttttgtat
     2101 ggaggacaaa cattgttcca gaaagaaccg gacattgtaa tttactatga tgatggacaa
     2161 ttgcatacag ggtgcaaatt agtcaaaacc aaacaatcat tgccaaataa tgaatgcttg
     2221 gtttatttct ttggtgagaa tttatttatt tcaacagaaa gtcgaaaacc attttaaagt
     2281 atcttttcaa caaggagaca atgatgaccg ttaaagcgga agcgagtatg ataccggata
     2341 tcataaaaat gatgaaatat ggtcgtaggg aatacatcat gatactgtaa gcttcttcag
     2401 gcgtatgcat ctgccagtat gccttatgta ttccggctac aacaaggctg atccagaata
     2461 tgaataatgc acccactgct atataaaggc tgatgcttat ggtgcgggaa ggcttggcaa
     2521 tgtgcatgtc aaaaatatct gctgcaaagg ctagtaaaag aaaagtgttg atgccaatgg
     2581 tggcccccat tgtatgtcct acagttatat gtgtgccatg tgtgtaggca ttaattgcag
     2641 gaatggacat ggcaatagcc aaaattaagg tcagaaatat ccagatatca gctgcaaaca
     2701 taaagcgata ggggaggatg tggtaatgcc tcatcgctgt actgaccgaa gaccgccagt
     2761 aatatatgat tctgcccagt aataaaagct ctgtcatgct gaccagatag ctgatgtatt
     2821 ggataatctt tgttccgggt aaggtgtaga tatgatggcc ccagttgaac atcagattga
     2881 agaggcccag aaaataaatg gaaaaggcaa tgccggagta actataacgt tgtgttccgg
     2941 aaatcttatc catcagaaag atgcttgtcc cataaattag catgttccac ccgccaacca
     3001 tcgagccata tgatttccat tgtatggtca tatcgtgaat aataccggaa cgaaatgcgg
     3061 gaatcaacca aagataagat tcgatatagg taaatagaaa aaaagctgct ccggttagcc
     3121 acatccatac atatacaggt tgatgtctga atgatttgat cgacttaaag acattgatga
     3181 tgaataacag ccaggcagca ataataggaa tcgccagaag aggattgaat tcccagtatt
     3241 ctctgcctcc aaacataccc ataacatatg aaaccaggat ggcaatcata gaggtggcaa
     3301 aaaggaagcg ttgccattta gccaaccttt cagaccataa tctgccctgt gactctgaaa
     3361 tgtagtgtag tacagaacca catgctccca tcaaaatcca gaataccaca gatgaaacat
     3421 gtaaaggcct gactttgtca aaccccaaac cgggtttgaa taatccggga attacatact
     3481 ggatggctcc tatggtgcca aaaatcagtc ccgaaaacat gaagattaga cccaacgcca
     3541 caaaccctgc acctacagat tgtttacctt tcaattgttc catcaatatt cagttttaac
     3601 ttagcaggag aagcaatccc actttcatcc agacttttaa gataagccaa tatttcttcg
     3661 atttgcgtct cactaagctg gtaagcgggc attatatctg ttccatactg aattcttgag
     3721 atgataaagt cttttgggcg atgagagtat acattcgtta gatcaggtcc aaggtatcct
     3781 ccaagtccat atatttgatg acaggcagta caattcttgt cctgccatat ttgcttgcct
     3841 ttatcagaca tcaatccgga gtgtgcattc ggatcgtagt tcctgttgta caagtaggca
     3901 gtataacaaa agaataaaat gcttaatact gctaaaatcc ccacttttat tttgtcagcc
     3961 atctatgtgt gaattttcca ggcgtgaata taaggcaaat ttcaattcaa gttgggtaat
     4021 caaaacttct taatgagatt tttaaaagat tatttaatta aatatatttt ataaattaca
     4081 gaaaaacagt ttttcattat tattatatta aagtaatact ttaatattaa cctcctgagt
     4141 tcaaagcgct attttacttt tttcgtttgt gtcttaatgc tgaaaaatat tacctggcat
     4201 ttcttagctt gtttttcttt ctactttatc tatataggat cactccgtcc gtttttttca
     4261 ggaaaatttt ataatgtaga tttatattat agaataaaag tacttaaata cttttatttg
     4321 taaaattgaa ttatatttgc attgattttt taagctatta actgtataca taattatgtc
     4381 tgcaaccaat gaaactgtaa taaatgttca ggaaattgaa ccaagattga gacaccttac
     4441 catcttccag gcttttgata gtcttgagga aggagaaagt ctcgtaattc ataataacca
     4501 tgatcctaaa ccggtgtatt accaattggc tcggttgaga ggagaagttt tcgattggga
     4561 gtatcttgag gcaggcccgg agtggtggga tgttaaggtg acaaaaagac ccgatgcaga
     4621 tacttacttc gaaaatcaaa cttacgagga gattgtaatc gatgtcccat ctttggaacc
     4681 aagagttaag catgctacaa tttttcagac tttcaaccag ttaagacccg gacaatcctt
     4741 tataattcat aataatcatg atccaaggcc ggtctactac caattaatgg atctacgggg
     4801 caacatcttt ttgtgggagt atttacagca aggcccggaa tggtgggata ttcgcgttac
     4861 ccgtacagtg cctatgattc cgatggagga gggcaggatg attatcaatg tgccagccat
     4921 cgatcctgta cacaagcacg gtacgatatt ccatgtcttt gagaacatga accccggtga
     4981 atcattcatc attcataatg atcatgatcc caagcctctg ttttatcaat tgaaggacag
     5041 gcatggagat tcttttacct gggattatat catgcagggg cctcactggt ttgatatctt
     5101 gattaccatt aaggaaccgt ccataacagc ggacaaggag gaggttatta tcgatattcc
     5161 ttcaattgaa cccgcaaaaa agcatgccac tatcttccgt atgtttgaag aattggagga
     5221 aggaaaatca ttcatcattc ataataatca tgatcccaag cctgtatatt accagatgct
     5281 ggagatgttc ggtgatatat ttgattggga gtatttgcgt caaggtccac agtggtggga
     5341 tatccgtgtg acaaggagaa agtcggtaac acaaccaaat caaaggcaac ccatcgacaa
     5401 tgatgatgca atcattgtcg atgtaccatc cctcgagcct aggctgaagc atgtaaccat
     5461 ttttgaaact tttgacaagt tagaaccagg tgaaagcctt attattcaca atgaccatga
     5521 ccctaagccg gtttattatc aattgcaggc tgagcgtggc gatgtgttta cctgggaata
     5581 tctgcaacaa ggcccacagt ggtgggatat ccgcgtgaca agaaagggaa ctgaaaccaa
     5641 tgaaacaatc ggtgagatgg ttgccaaaga cttgcgcaag gctgatattt tcaaaaaata
     5701 tgggatagat ttctgttgca atggcaagaa gacagttcgt caagcctgcg ctgataaagg
     5761 tattgatcct gtagtcgtcg agcgggaact gcagcaacca acccaagaat ctgcatatgg
     5821 tcatcaactg aactataatg aatggacccc tgattttctt gctgattata ttattaat
//
LOCUS       DAOQQN010000195         5847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5953_length_5847_cov_5.265366, whole genome shotgun sequence.
ACCESSION   DAOQQN010000195 DAOQQN010000000
VERSION     DAOQQN010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5847
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5953_length_5847_cov_5.265366"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLG24_08110"
     CDS             <1..384
                     /locus_tag="PLG24_08110"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HMZ24893.1"
                     /translation="NPVYSDVVTEVLDYFIQKLEHFTQIGLVDVIFDPGFGFGKTLEH
                     NYTLLNNMEVFRITGKPILAGLSRKGMLWRPLNSDPSKVLPATIAVNLLALQHGATFL
                     RVHDVPENVQLLETFQIIKQFENKY"
     gene            400..5310
                     /locus_tag="PLG24_08115"
     CDS             400..5310
                     /locus_tag="PLG24_08115"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HMZ24894.1"
                     /translation="MEENNNKIKATKDKRSLPGLISHFLWSLTKYSFIFLILMMMGVY
                     FLIQLPSFQNYAATKATEFLSRELKSEVTVGRIRINFFQKVLLEDFVVKDRQQDTLLN
                     IGELKAELKGGFLGLLDGKINITSAFIRNLDAHLVENCPKYDNNYQFLLNYLENGHPD
                     SLFSRPTGKQNKISLNLNNVELQDVSFNYLSKSKGKIVIASLDRLTADFNEFNLDSNL
                     ILVNRLKIDAPVYKIENIEPIPCKEVRYLNPYASLEAELKKRRKPKPNLHIEAQDISI
                     EEGIFSYHNFRHPPIRANAGKVLDFQNIDLSDVNVGLETFIFEGNSITAALTQLAVKT
                     GTPFNINEFSADRISYSPTKIGVENFDLKTDYSHLQDTLSLHYKSMEDFQDFVNKVRM
                     AGRFKNSEISVKDIMVFDPKLETVDFLRHNKDKEIGLDGSFAGTVNNLKGNNLKLEVG
                     KYASLDGNFSLHDITNIQQATLNLNLNTLKTNADGLTAIIPGFKAPEALKKLGTINYS
                     GKLDGYILDFVTYGKVKTAQGDANVDIHLDIRDGAENAKYSGIIGLSQFNLGNLTGNQ
                     DLGKVTGTFTIKDGQSFSLESISTELSTEVQSFTYKGYDYKNFKIDGKLLKNTFDGKL
                     VISDPNANLKFDGAIDFSKDRKVLNFNSEIYNLDLSKLNLANLSWKIKGTINSNLTLS
                     DLSDMEGSFIAQNLDIKDTSGHHLELDYLNFNAIKSGNGLKEYTLTSNMVDLDLIGKF
                     EIEQIGQALIKVFHYNHTALANQLGIQSRVQNIYNNDFNFNLNVKDSKELLNLLGTSL
                     QPVQGSRISGYFSNIDSTKYRININGNIPVLRSDNFNFNYLFFEGIGNQESSEYFIFA
                     NSGNILDRSLSQMDLSATLIVDSVRFNIKTPQIQKIAENLSIAGIYTVENGYNILKFN
                     QSKFDFFDDGWTVNKDNLVKFGKNELYLKNLTATNGRQEVIFNSYGKQGLEVNVKNFN
                     AAVLDSLLANADLKIIGTGDINLRMDSVFTQQNILLTSHFDSVNVNGIALGKLNLEAN
                     AQSLKSKVGFDLSMGELNKELHVTGLYTLPQYEAFDYPPNYIDLLISSESYPLVIADI
                     FLGEMIKDTRGTFTTNFRIKGKTNQLDLNGTALVQNMGTTIRYLGTRYYVPKHTVRIN
                     NNMIDLDGLTLLDERGNIASVSGGIKHNRFKNLNADIEINSDNFLMLKTTEKDNPNYY
                     GTASGRFTGKFKGPFDQLNIEIEATTGNGTDLFLPIGTSKEVDALSFIEFKNRFKEDV
                     EKEINKVVTSKGLAFKMNAEVNEKATLNLIIDKQTGDIIKSKGNGILEIAIPRNGNLS
                     MYGGYEVAGGEYRINLIPLYNLSIFGAKFLIKKGGTLIWNGDPINAQVNIDADFKGVN
                     TSPYNFISEYIPEGDTKLQSEANRPTPVDLTLNLRGDLLKPEITFKIDFPQLSPDLRT
                     YVDSKLRSLEQDPNELYKQAASLVSFGSFIPKDNFNLSAVTATAYNTLSDLISSQLTQ
                     ILSPMLTAAVADGKILTGVDLNLNYNFYSANTSQGTNIQNRIGSELLIGPSLKFFDGR
                     LIVNTGVKSGEQGKNQNYVAGDVDLQYYLTTDRKYVLRLYQNNDAVLEGRRIKSGVGI
                     SFQKSMDSIAELFRREQKKKHTKTRD"
     gene            5327..>5847
                     /locus_tag="PLG24_08120"
     CDS             5327..>5847
                     /locus_tag="PLG24_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HMZ24895.1"
                     /translation="MTIKIQHICLQIILVISSYFQVFGQTTNNRIEIFFTRPITARYQ
                     NPNLFPYGSGGSVTKQKIIDEIKNAKSTVDVCVYNNTENDIVDALKLVKAKGVRIRYI
                     TDSGTGNSALKGNPGFPILYLGKNGGIMHNKFAIVDAKRVETGSFNYTNHASEANNEN
                     QIYLSAPAVVARYR"
BASE COUNT         1965 a         1157 c         1148 g         1577 t
ORIGIN      
        1 aacccggtct acagtgatgt agtgacagaa gtattggatt atttcataca aaagctggaa
       61 catttcacac agatcggctt ggtagacgtt atttttgatc ccggattcgg gtttggaaag
      121 acattggaac ataattatac gttattgaat aatatggagg tttttcgaat tacaggcaag
      181 ccaatactgg caggactgtc ccgtaaggga atgttgtggc gaccattgaa ttccgaccct
      241 tcaaaggtat tgccagctac tattgcggta aatctgttag ctttgcaaca tggtgctaca
      301 ttcctgaggg tacatgacgt gccagaaaat gtacagttgt tggaaacgtt ccagatcata
      361 aaacaatttg aaaataagta ctgataccgg ggaaaatagg tggaagagaa taataacaaa
      421 ataaaggcga ccaaggacaa aaggtcatta ccgggcctga tatcccattt cctgtggagt
      481 ctgaccaaat attcctttat tttccttatt ctgatgatga tgggcgtgta tttcctgatt
      541 cagctaccct cctttcagaa ttacgctgca acaaaagcaa cagagtttct aagccgtgaa
      601 cttaagtctg aagtaacagt cggaagaata agaattaatt tctttcagaa agtcctgctt
      661 gaagactttg tcgtaaaaga cagacaacaa gacactttac tgaatatagg ggaattgaag
      721 gctgaattga aaggtggctt tttgggcctc ctcgatggta aaattaatat cacatccgca
      781 ttcatcagaa atctggatgc ccacctcgta gaaaattgcc cgaaatatga taataattac
      841 caattcctgc tcaattatct cgaaaatggg cacccggatt ctcttttttc cagaccgacc
      901 ggcaaacaaa acaaaataag cctaaaccta aataacgttg agttacagga tgtatcgttc
      961 aattatttaa gtaaatccaa aggaaagatt gttattgctt cgctggatcg cctcactgcc
     1021 gatttcaacg aattcaacct ggacagcaac ctgattctgg tcaacagact caaaatcgat
     1081 gccccggtgt acaagataga aaatattgaa cctataccct gtaaagaagt cagatacctc
     1141 aatccatatg cttccctgga ggcagaatta aaaaagaggc gcaaacccaa accaaacctg
     1201 catattgaag cacaggatat aagtattgaa gaaggaatct tttcttatca caatttcagg
     1261 catccaccga tcagggccaa tgccggcaaa gtcttggatt tccagaatat agatttgagt
     1321 gatgttaatg tcggcctcga aacttttatc ttcgaaggta attcaatcac cgctgccttg
     1381 acgcaactgg ctgtaaagac aggtacgccg ttcaatatca atgaattcag cgctgatcgt
     1441 atttcttact ctcctacaaa aattggggtt gaaaactttg acttgaagac agattattcc
     1501 cacctgcaag atacgctcag ccttcattat aaatcaatgg aagacttcca ggactttgtt
     1561 aataaggtca ggatggcagg tcgattcaaa aatagtgaga tctctgttaa agacatcatg
     1621 gtattcgatc ctaaattgga gactgttgac ttccttcggc acaataagga caaggagatt
     1681 ggactcgacg gatcctttgc cggaaccgtc aataacctga agggcaacaa cttaaaactt
     1741 gaagtcggaa aatatgcaag cttagatgga aatttttcat tacatgacat tactaatata
     1801 caacaggcaa cgctcaacct aaacctaaat actttaaaaa caaatgctga cggccttaca
     1861 gcaatcattc ccggatttaa agcaccggaa gccctgaaaa aactgggcac catcaactat
     1921 agtggaaaac tcgatggcta tatccttgac tttgtaactt atggcaaagt aaaaactgcg
     1981 cagggggatg ccaatgtgga tattcatctt gatattaggg atggtgcaga aaatgcaaaa
     2041 tacagtggaa ttatcggatt aagccaattc aaccttggaa acttgactgg caatcaggac
     2101 cttggaaaag tcaccggaac ctttacaatt aaagatggac agagcttttc actggaatca
     2161 atcagtacag aattgtctac cgaggtccaa tcttttacct ataaaggtta tgactacaaa
     2221 aactttaaaa ttgacggaaa gcttctgaaa aatacttttg acggaaaatt ggtcatttcc
     2281 gatcccaatg ctaatttaaa attcgatggt gccattgatt tttccaaaga caggaaggta
     2341 ttgaatttca attcggaaat ttacaatctt gatctttcaa aattaaacct tgccaattta
     2401 agctggaaaa ttaaaggaac cataaactca aatctgacac tgagcgacct aagtgatatg
     2461 gagggaagtt ttattgctca gaatttagat attaaggaca cctccggtca tcatcttgag
     2521 ttggattatc tcaatttcaa tgctattaaa agcgggaatg gtttaaaaga atataccctt
     2581 acttccaaca tggtcgattt agacctcata ggcaagtttg aaatagaaca aatcggacaa
     2641 gctctgataa aagtctttca ttacaaccat accgcattgg ccaaccagtt gggcatccaa
     2701 tccagggtgc agaacatata caacaatgat ttcaatttca atcttaatgt caaggattca
     2761 aaagaacttc taaatctatt aggcacatcg ttgcaacctg ttcagggaag ccggatttcc
     2821 ggctatttct ccaacattga ttctactaaa taccggatca acatcaatgg gaatataccg
     2881 gtcttacgtt ccgataattt taacttcaac tatctttttt tcgaaggtat tggcaatcag
     2941 gaatcctctg aatacttcat ctttgcaaat agcggcaata ttctggacag atctcttagt
     3001 caaatggact taagtgcgac attaattgtg gacagcgtcc ggttcaatat taaaacacct
     3061 caaattcaaa aaatagctga aaatctaagt atcgcaggta tctatactgt agaaaacggc
     3121 tataatattt taaaattcaa tcaatcaaaa ttcgatttct ttgacgatgg ctggacagtc
     3181 aataaggata acctggtgaa attcgggaag aacgaattgt atttgaaaaa cctaacggct
     3241 actaacggca gacaagaagt gattttcaat agttatggca aacagggatt ggaggtaaat
     3301 gtcaagaact ttaatgcagc ggtgttggat agtttattag ccaatgctga tcttaagatt
     3361 attggaacag gagacattaa ccttaggatg gacagtgtat tcactcaaca aaatattctg
     3421 cttacttccc actttgacag cgtaaatgtc aatggaatag cattgggcaa attgaacctc
     3481 gaagcaaatg cacaatcact gaaaagcaaa gtaggattcg atctgtcaat gggagaatta
     3541 aataaagaac tacatgtaac aggcctttac accttgccac aatatgaagc attcgattac
     3601 cccccgaatt atatagacct gctgataagt tctgaatcat atcctcttgt cattgcagat
     3661 atttttctag gtgaaatgat aaaagataca cggggaacat ttaccactaa tttcaggata
     3721 aaaggcaaaa caaatcaact cgatctgaat ggaactgctc tggtgcagaa tatgggtacg
     3781 accatcagat accttgggac aagatattat gtacctaaac ataccgtccg gatcaataac
     3841 aacatgatag atctggatgg cttgacccta ttggacgaac gaggaaatat tgcatctgta
     3901 tcaggaggaa tcaaacacaa cagatttaaa aaccttaatg ccgatatcga aattaattcc
     3961 gacaatttcc tcatgctgaa gactactgaa aaagacaatc ccaattatta tggaacagct
     4021 tcgggacgtt ttacgggtaa gtttaaaggg ccttttgatc agctcaatat tgaaatcgaa
     4081 gcaacgacag gcaatggtac ggatctattc cttccaatag ggacatcaaa ggaagtagat
     4141 gcactttcct ttattgaatt taaaaataga tttaaggagg atgtggaaaa agaaatcaat
     4201 aaagtcgtaa catcaaaagg cctggccttc aagatgaacg ccgaagtgaa tgaaaaagca
     4261 accctgaatc tgatcatcga caagcaaacc ggtgatatca tcaaatcgaa gggaaacggt
     4321 attcttgaaa ttgcaattcc ccgaaatggc aatcttagca tgtatggagg atatgaagta
     4381 gccggcggtg agtatagaat caatcttatc cccctgtaca atctttctat ttttggtgca
     4441 aaattcttga ttaaaaaagg gggaacgctg atatggaatg gcgacccaat caatgctcag
     4501 gtcaatatcg atgccgattt taagggggtc aatacttcac cgtataattt tatcagtgaa
     4561 tatatacctg aaggtgacac aaaattgcag agtgaagcca atcggcctac cccggtggat
     4621 cttactttga accttagggg agacttactg aaaccggaaa taacctttaa aatagacttc
     4681 cctcaacttt cccctgacct aagaacctat gtagatagca aattgcgttc gctcgaacag
     4741 gatccaaatg aattgtataa acaggcggca tcgttggttt cttttggttc atttataccc
     4801 aaggacaact tcaacctgtc ggcagttact gcaactgcat acaacactct ttctgacctt
     4861 atttcctccc aattgacaca aatcctttct ccaatgctga ctgcagcggt agcagacgga
     4921 aaaatattga ccggagtgga ccttaacctc aattacaatt tttattcagc caatacaagc
     4981 cagggaacta atattcagaa caggatagga agcgaattac ttataggtcc cagtctgaag
     5041 ttcttcgatg gtcgcctcat tgtcaataca ggcgtaaaaa gtggtgagca gggcaaaaac
     5101 caaaattatg tcgcaggtga tgttgacctg caatactatc tgacaactga tagaaaatat
     5161 gttcttcgtc tttatcagaa caatgatgca gtattggaag ggcgtcgtat caagagtggg
     5221 gtcggaatct ccttccaaaa atctatggat tcaattgcag aattattcag aagagaacaa
     5281 aagaaaaaac acaccaaaac cagagattaa ttttttaatg caagcaatga ccataaagat
     5341 tcagcatata tgccttcaaa ttatattggt aatctcttct tactttcaag tattcgggca
     5401 aacgacaaat aacaggattg aaatattttt tacccggccc attactgcaa ggtaccagaa
     5461 tcccaatttg tttccatatg gctccggggg atctgtcacc aagcaaaaaa taattgatga
     5521 aatcaaaaat gccaaatcaa ccgtcgatgt gtgtgtttac aataatactg aaaatgatat
     5581 agtagatgct ttaaaactcg tcaaagcaaa aggagtaagg atcagatata taacagattc
     5641 cggaactggc aactcagctc ttaagggcaa tcccggtttt ccgatacttt acctcggaaa
     5701 aaatggtgga atcatgcaca acaaattcgc cattgtggac gccaaacggg tcgagaccgg
     5761 ttcgttcaat tatacgaatc acgccagcga ggccaataat gagaatcaga tttacctttc
     5821 cgcccccgcc gtcgtggccc gctaccg
//
LOCUS       DAOQQN010000196         5843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5960_length_5843_cov_5.009502, whole genome shotgun sequence.
ACCESSION   DAOQQN010000196 DAOQQN010000000
VERSION     DAOQQN010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5960_length_5843_cov_5.009502"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1387)
                     /locus_tag="PLG24_08125"
     CDS             complement(2..1387)
                     /locus_tag="PLG24_08125"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HMZ24896.1"
                     /translation="MSNNEYIAANRDRFLNELVEWLKIPSISSDSRAKEDMGRAAEYL
                     KARLVEQGVDRAEIYPTAGHPIVYAEKITDPAKPTVLVYGHYDVQPPDPLDLWDSPPF
                     EPVIKITDEHPEGAIFARGSCDDKGQVYMHVKAFEVMAKNNDFPCNIKFMIEGEEEIG
                     SVHLGKFCEDHKDMLKSDVILISDTSIIDNQTPSITTGLRGLAYMEVEVTGANRDLHS
                     GVYGGAVANPINVLAKMIAGMHDENNHITIPGFYDAVENLSREERDAMAEAPFSLEGY
                     QRDLGIDSIYGENGYTTLERASIRPTLDVNGIWGGYIGEGAKTVLPSKAFAKISMRLV
                     PHQNSTVISKLFSDYFTSIAPKGVKVKVTPHHGGEPVVTPVDSLEYKAAAMAMKDTFG
                     TEPIPQRSGGSIPIVALFESVLGTKSVLMGFGLDSDDIHSPNEHYGVFNFFKGIETIL
                     RFYPNYAALKQ"
     gene            complement(1696..2541)
                     /gene="prmC"
                     /locus_tag="PLG24_08130"
     CDS             complement(1696..2541)
                     /gene="prmC"
                     /locus_tag="PLG24_08130"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HMZ24897.1"
                     /translation="MNTPSRDLPSLVSALTGHYGNRESENILRILYEDYLILPRKTAL
                     EDIPPSKVIKLKAALDLLLKDYPIQYITGKVWFYGYLFLVGPGVLIPRPETEELVELA
                     LKNINKSDSCRVIDIGCGSGIIPIVIKRQRPGVMVDAVDISQQAIEFTRKNAELLQVE
                     LNIFEANILHPEWTFFEDYDFIISNPPYILEDEIPEMSVSTVLYEPHIALFSGEAMTF
                     YKAILFFGQKKLKIGGKILLECSEFHAEEVRNLALSMGYSEAYLHKDLQGKDRMIEVV
                     NSNSF"
     gene            complement(2641..3117)
                     /locus_tag="PLG24_08135"
     CDS             complement(2641..3117)
                     /locus_tag="PLG24_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24898.1"
                     /translation="MTKYFDSISSDEKKVLKDTFVYITLLIASAENKLTLAEKEAAIK
                     TIKVRGYEANSLFNEFYDEINNTFAEELEQASAEHKISRDEIDYFSRKISQVNEVLEK
                     LPSYVARQLYNDYKRFSHRIANADGGILGFAKVSIEERNVVDLPMIHQIEFDDEEE"
     gene            3239..>5843
                     /gene="mutS"
                     /locus_tag="PLG24_08140"
     CDS             3239..>5843
                     /gene="mutS"
                     /locus_tag="PLG24_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HMZ24899.1"
                     /translation="MTKSNKEQVGNKNDGVTPLMRQYNDIKSKYPDALLLFRVGDFYE
                     TFGDDAIKVAGILGIILTSRNNGGSDTELAGFPHHSLDIYLPRLVKAGYRVAICDQLE
                     KPSKEKKIVARGVTNVVTPGLTLDDSILQSRTNNYLCAIDIRQTDHFGIAFLDVSTGE
                     FMVAEGGEAVIESLFQRFKPAEVIFNKKDKKNIQQLIKSQFPVFPLDDWVFSHESSYN
                     KLTTHFQSNTLKGFGIEELHSAQIAAGAILQYLEMNENKEISHINRIARIPQESYMWL
                     DRFTIQNLELVSELHSDGRSLVDILDHCNTPMGTRQLRKWVLLPLLSVEAIEYRLTIV
                     EYFVRHSPERDGFIQLLKQIGDLERLISKSAMGRIQPREVLQLKKALIAISELKKSLD
                     NTESVELQHIGDALHDCHQLISKIDSTLRDDAPTLVSKGNVIKNDVDAELDEYRYLIG
                     HSKDVMAEILQKETEASGIQNLKLGFNNVFGYYLEVTAKHKHTTVPDHWIRKQTLANA
                     ERYITEDLKILETKILQAETVIQEIEERLFTRLIIQINEYIESIQANCAQIARLDCLI
                     SFAYQAGRYHYNKPEFNDDGIIRIIQGRHPIIEVMLGHEKKYIPNDLYLDQESQQIII
                     LTGPNMAGKSAVLRQTGLISLMAQIGSFVPAESASLSIVDKLFTRVGASDNISGGEST
                     FMVEMNETASIMNNLTDRSLILLDEIGRGTATYDGISIAWALAEYLHDNDAGRPKTLF
                     ATHYHELNELTYKLPRVKNYNLSVKETKDKVIFLYKLAEGGSEHSFGIHVARMAGMPQ
                     RIIDRSNQIMKHLEDQSGSLSGSKKKETKEILQTLKAESYQLSIFETDDPNIGEIRNI
                     LLDIQPEIMTPI"
BASE COUNT         1776 a         1270 c         1157 g         1640 t
ORIGIN      
        1 tttattgctt caatgctgca tagtttggat aaaaccgcag gattgtctca atacccttga
       61 agaaattgaa tactccgtaa tgttcattgg gcgaatggat atcgtctgaa tccagtccaa
      121 agcccatcaa taccgattta gtacctaata ccgactcgaa aagggcaact atcggaatac
      181 ttccaccact acgctgagga ataggttccg taccaaaagt atccttcatg gccatcgcgg
      241 cagccttata ctccagtgag tccaccggtg taacaaccgg ttctccccca tggtgtggag
      301 taaccttgac cttcacgcct tttggggcta tggaggtaaa gtaatcacta aataatttac
      361 tgattaccgt agaattctga tgaggaacca gcctcatgct gatcttggcg aatgccttgg
      421 acggcagtac cgtcttggcg ccctcaccga tatatccacc ccagatgcca ttgacatcga
      481 gagtcggcct aatagacgcc ctttctaaag ttgtatatcc gttctcacca tatatcgagt
      541 caataccgag gtcacgctgg tagccctcca aagaaaaagg tgcttccgcc attgcatctc
      601 gttcctcgcg ggacagattc tcgaccgcat catagaatcc gggtatagtg atatggttat
      661 tttcatcgtg catcccggcg atcattttgg ccagaacatt aattggattg gctaccgccc
      721 cgccatatac tccggagtgc aaatccctgt tggctcctgt cacttcaacc tccatatatg
      781 caaggcctcg taaacctgtc gtgattgaag gcgtctgatt atcaataata ctcgtgtcag
      841 aaatcaggat aacatcagat tttaacatgt ctttatgatc ttcacaaaat ttgccgagat
      901 gtacggagcc gatctcctcc tctccttcaa tcataaattt gatgttacac gggaagtcgt
      961 tgttctttgc cattacctcg aaagccttga catgcatata cacctgtcct ttatcatcgc
     1021 aagatccccg tgcaaagata gcgccttccg gatgctcatc cgtaatcttt ataaccggct
     1081 caaatggtgg tgaatcccat agatccaacg gatccggtgg ctgaacatca taatgaccgt
     1141 ataccagaac cgttggttta gccggatcgg taattttttc agcatataca atgggatgtc
     1201 cggcagttgg atagatctct gcccggtcca ctccctgctc aaccaatctt gctttcaagt
     1261 attcggcagc acgtcccata tcttcttttg ctcttgaatc tgagcttatg gacggaattt
     1321 tcaaccattc gaccaattca ttgagaaaac ggtcgcgatt ggctgctata tattcattat
     1381 tactcatatt attaaatttt tataataaag agtgcaaaag tacaaaaaat agctaatgtt
     1441 ttgtggctat taaaaagtta taacggaaaa aagatatgga aattttcagg gatgaataat
     1501 tattgcaaaa agggagataa atatagtccg acaatataat atctttatta catgtttatt
     1561 ttactaaaat atttaaattt ataaatataa ttaatatatt ttatttaata aattgttgat
     1621 cataatttta tccttactct cattgaggtc aataattgaa tatttccaat tttaaggtga
     1681 aaggaaattt cgtgttcaaa aactgtttga attcaccact tcgatcatcc tgtcctttcc
     1741 ctgcagatct ttatgcaaat aggcttcgga ataccccatg ctcagggcaa gattcctaac
     1801 ctcttctgca tgaaattcgc tgcactcaag cagtatcttt ccgccgattt taagcttttt
     1861 ttgtccaaaa aacagaattg ccttatagaa agtcattgcc tcccctgaga atagggctat
     1921 atgaggctca tacaagacag tagatacgga catctcaggg atctcgtcct ccagtatata
     1981 gggtggattg ctaataatga agtcgtaatc ttcgaaaaat gtccactcag gatgaagaat
     2041 atttgcttcg aaaatattga gctccacttg aagtaattca gcattttttc gggtaaattc
     2101 aatcgcttgt tgagatatat caacagcatc aaccatgaca cccggccttt gtctcttgat
     2161 gactatcgga atgatgccgc taccacaacc gatatcgata acccggcagg aatcagactt
     2221 attgatgttt ttgagggcta attcgaccaa ctcttcggtt tccggtcttg gaatcaatac
     2281 accggggccc actagaaaaa ggtagccata aaaccagact tttccggtaa tatattggat
     2341 tgggtaatcc ttcaacaaca agtccaatgc tgctttcaac ttaattactt tggatggggg
     2401 aatatcctct aatgccgttt tacgtggcag aatcaagtaa tcctcataga gaatccgaag
     2461 gatattttct gattcccgat tgccataatg cccggtcaga gccgatacaa gtgaaggtaa
     2521 gtcacgggaa ggagtgttca ttcttttgct tcgtaaggca attactcaaa gaatattgtc
     2581 cggccaggca tctccaccga aatacaagtc ctgacaaagg atgaatcctg tcaatctcag
     2641 ttactcttct tcgtcgtcaa attcgatctg atggatcata ggcagatcca ctacatttct
     2701 ctcttcgatg gacaccttgg caaatccaag aattccgcca tcagcgtttg ctatacggtg
     2761 agaaaaccgt ttgtagtcat tgtaaagttg tcttgcaaca taagaaggca atttttcaag
     2821 tacttcattg acctgactga tcttacggga aaaataatca atttcatctc tgcttatctt
     2881 atgttccgcg cttgcctgct ctaattcttc tgcaaatgta ttattgatct catcataaaa
     2941 ctcattgaat aagctatttg cttcataacc tctcacctta atagtcttga tagcagcttc
     3001 cttttcagca agcgtcaact tattttctgc agaggcaata agcagggtaa tgtaaacgaa
     3061 agtgtccttt aaaactttct tttcatcaga ggaaatcgaa tcaaaatatt tcgtcatgtt
     3121 taactattta tgactaaatg aattaatgaa aaatcactta ttcgaatgaa taaatttata
     3181 taaatttttc gcaaatatat agtgttgttt ctaattttaa ccgtttaaaa taaaaatttt
     3241 gacaaaatca aacaaagagc aagtcggaaa taagaatgat ggtgtgacac cattaatgcg
     3301 gcaatataac gacatcaagt ccaaatatcc ggacgcattg ctgttgtttc gtgtgggtga
     3361 tttttatgag actttcggag atgatgccat aaaagttgcc ggcattcttg gcatcatctt
     3421 gacaagccgc aataatggcg gttcagatac tgagttggca ggatttccac atcattcgct
     3481 ggatatatat ctccccaggc ttgtcaaggc aggctatcgg gtcgccatct gtgatcagct
     3541 tgaaaaaccc agtaaggaaa agaagattgt cgctcgtggg gtaaccaatg ttgtcacacc
     3601 cggattgacg ttagacgatt ccattcttca atcccgtacc aataattact tatgcgccat
     3661 tgatatccgc cagactgatc attttgggat cgcttttctg gatgtatcaa ccggcgaatt
     3721 catggtagca gaagggggtg aagcagtgat agaaagcctt tttcaaagat tcaaacctgc
     3781 tgaggttatt ttcaacaaga aagacaagaa gaatattcaa caactgatca aatctcaatt
     3841 tcctgttttc ccgttagacg attgggtttt cagtcatgaa tcttcataca acaagttgac
     3901 gactcacttt caaagcaaca cactaaaagg attcggcatt gaagaattac attcagccca
     3961 gatagcggca ggtgccatcc tccagtatct tgaaatgaat gagaacaagg aaatcagcca
     4021 catcaacaga atagcaagaa ttcctcagga atcctatatg tggctggacc gatttaccat
     4081 tcaaaatctc gaattggtca gtgaactgca cagcgatggc cgcagtttgg tggacattct
     4141 tgatcattgc aatacaccga tgggcacccg acaacttcgg aaatgggtat tgttaccttt
     4201 attatcagtg gaagcaatag aatacaggct cacaatagta gaatattttg tccggcattc
     4261 cccagaaagg gatggattta tccaattgtt gaagcagatc ggtgaccttg aacgattgat
     4321 ttcaaaatca gctatgggca gaatccaacc gagagaagtt cttcagctta aaaaggcact
     4381 cattgcaatt tcagaactga aaaagagcct tgacaataca gaatcggtag aactgcaaca
     4441 tatcggcgat gcattgcacg attgccacca actcatcagt aaaatcgact caaccttgag
     4501 ggatgatgct cctaccctcg tatcaaaggg caatgtgatc aagaatgatg tcgatgccga
     4561 attggatgaa tacagatatc taatcgggca tagcaaagat gtgatggccg aaatactcca
     4621 aaaggaaacg gaagcttcag gtatacaaaa cctcaagctg ggattcaata acgttttcgg
     4681 atactatttg gaagtaaccg caaaacacaa acacaccaca gtacccgacc attggatcag
     4741 gaagcagacc ttagccaatg cagagaggta tattacagaa gatcttaaga tcctggaaac
     4801 caaaatattg caggctgaaa ctgtcattca ggagattgaa gaacgcttgt ttacccgctt
     4861 gattattcaa atcaatgaat atatagaaag tattcaggct aattgtgccc aaatagcgag
     4921 gcttgactgc cttatctcat ttgcatatca agcaggcaga taccattaca acaagccgga
     4981 attcaatgac gatggaatca tccggatcat tcagggtcgg catcccatta ttgaagtcat
     5041 gctcggccat gaaaagaaat acatccccaa tgatctttat ctcgatcagg aaagtcaaca
     5101 aatcatcatc ctgacaggtc ccaatatggc cggaaaatcg gctgtactgc ggcagacagg
     5161 actaatttcg ctaatggcac aaattggttc ttttgtaccg gcagaatcag ccagcctgag
     5221 tatcgtggat aaactattca cccgcgtagg tgcatctgat aatatttcag gtggtgaatc
     5281 aacctttatg gtcgagatga acgaaacagc cagcattatg aacaatctta ccgaccgcag
     5341 tcttatcttg ctggatgaaa tcggacgggg cactgccacc tatgatggaa tctccatcgc
     5401 ctgggcattg gccgaatatc tgcatgacaa tgatgcaggc agaccaaaga cgctcttcgc
     5461 tacccattat catgagttga atgaactaac atacaaattg ccaagagtca aaaactacaa
     5521 tctttctgtc aaggaaacca aggacaaggt gattttcctg tataaattag cagaaggcgg
     5581 cagtgaacac agctttggga tccatgttgc gcggatggcc ggtatgcctc aaaggatcat
     5641 cgatcgtagc aatcagatca tgaaacacct ggaggatcag tctggttcgc tatccggctc
     5701 taaaaagaaa gaaaccaaag aaattctcca gacattaaaa gcagaatcct atcagctttc
     5761 catctttgag actgacgatc ccaacattgg ggagatcaga aatatcctcc ttgatattca
     5821 accggaaatt atgacaccga tag
//
LOCUS       DAOQQN010000197         5829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_5990_length_5829_cov_5.611015, whole genome shotgun sequence.
ACCESSION   DAOQQN010000197 DAOQQN010000000
VERSION     DAOQQN010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5990_length_5829_cov_5.611015"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="PLG24_08145"
     CDS             complement(<1..1345)
                     /locus_tag="PLG24_08145"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HMZ24900.1"
                     /translation="MKRRNFLRISTPAVLGPLVLEKSILKPFATGSLARIFNCEDVND
                     RILMIVQLKGGNDGLNCIVPTNQYDTYKNFRPTIGTSLDQLIPLDNTLSNDDHISLHP
                     GLISFKELYDQGCFTLVQAVGYANSNKSHFKGTDLWLSGGDSTPAHFNYKSGWMGRYL
                     DASYPGLAGEPTVDHPDPLGIQLGSKQQFLGFHTEHQHEAGINLSGQDPAGFYSLVSE
                     LGGIAPAHIPTSDYGNNIEYIIDIEHSTSRYSNRISEVFKKGINMGTYPDYDLADQLK
                     TVARMISGGGKTKIYVVSIGSFDTHQNQVEGSNPLDGTHGKLLTQLSESILAFQNDLR
                     MMQLDDRVVGVTFSEFGRRPKENGSKGTDHGTVAPMFLFGTPVRAGIVGKNIDLSQVG
                     SGNDIVGMGNDYRDVFTALLQDWLGASDRVLEDTYFGDYVTTKAPILESQYVVSPE"
     gene            complement(1375..2991)
                     /locus_tag="PLG24_08150"
     CDS             complement(1375..2991)
                     /locus_tag="PLG24_08150"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HMZ24901.1"
                     /translation="MASLELRKGILGERLAGHLLRRATYRYDIGRIKEFAQYTASEAV
                     DKLMLPVTFTLDQPIDPATGAPWINNGTSTTTAQPALRNYVRSWVLNEMRFDETIHGK
                     MVFFLHKCLVTTYNQSTSERFFDYLALLYKFTTGSFKVLARKMTMDNLMLIYLNNTNN
                     NKNAPNENYAREFLELFTIGKGPQVGPGDYTNYTEEDVVVGAKLLTGIRANTRLATID
                     PDTGLSTGRFAFGQHNTEDKTFSAAFDNRTIKGAKSVDEMPRELEEYVDMVFSKIETA
                     RLYCRRLYRYFISSNITPEIESDIIHPLAQQLMDNNYETASAIKTLLTSVHFYDEDDS
                     DNKDEIIGALIKSPLELFLGSVNFLHQKWPDQTTETASLYNFINRLAIAVILDNGALP
                     FFAPYDVAGYSAYYQEPVYDESWFTANTIVSRYKLCEMLLTGTSYLGGKSTKVTLDLP
                     AFVRNSGFFSNPGDPIQLTKEMANGMLPEPPDDDRINYFLNEMLDGIPPADWTYEWVA
                     YTSSGDPENVKIGLSKLLTAIMYSQEYQLL"
     gene            3252..5273
                     /locus_tag="PLG24_08155"
     CDS             3252..5273
                     /locus_tag="PLG24_08155"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HMZ24902.1"
                     /translation="MKKIFLQVFICLMCFSAINAQINYSDDFEAYAAGTTIVTNNPTN
                     WRTWGSTAGGAADDAYITDQFAASGKNSIKIKATSDNGGPEDIILKIGNNMKAGTIDV
                     SWNMLVAEGKVGYFNFQTTPNPGTGWAMEFFMNKDGSIDVVTNSKPFSNRLSYNPGKW
                     FNFRVVIDASNNLWQVYIDGECKGVFRNNLTTVSSIDFFAIANGEYYIDDVVLNSDAN
                     DVKVYSGVEAGIMNLNITEGIQIAGTNIKTSVQLYNVGADTITSIEYSIKNGGDVIEE
                     KLEGLSLAPNKSQNIAIPTAIKLADGDNALILTLTKINGKEDELSCNNILNASAYAVQ
                     PAKNRKVLLEEGTGTWCGWCPRGAVFLSQIYPSYDGYLVPIAVHNGDPMTVSTYDKEV
                     TSFNGFTGFPGMIVDRRIVVDPSAALAPGLGYLREEPDATLLIGATEPEGDGSMKVSV
                     SLDYLNEVPKGYSVVLTIVEDQVKGSTAQYNQANYYSGGGSGQMGGYENLPNPVPAAQ
                     MVYEHVARTHDHSVKNMDAYPAGEKAVLNYTVKLNSDWNQDEIKIVAFLLNTANQVSN
                     VDGVTLKDAYNNGFVSSTKEKILVNSGLTVYPNPVQGTTTIAINLDKPTDVTTNILDA
                     MGRIVLTKNYGKQNGHAVMNLDVNNLAPGSYTVLVRTTDGIATHKMVVQ"
     gene            5543..>5829
                     /locus_tag="PLG24_08160"
     CDS             5543..>5829
                     /locus_tag="PLG24_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMZ24903.1"
                     /translation="MSNYLTRRKSLTSLLSDASESEKGLKRTLSSTSLVALGIGAIIG
                     AGLFSLTGIAAAENAGPAVTISFVLAGIGCAFAGLCYAEFASMIPVAGSAYT"
BASE COUNT         1618 a         1173 c         1328 g         1710 t
ORIGIN      
        1 attccgggct cactacatac tgactttcaa ggataggagc tttggtcgtg acgtaatctc
       61 caaaataggt atcttcgaga acccggtcac tggcaccaag ccaatcctgc aaaagagcag
      121 tgaatacgtc acggtaatca ttgcccatgc ctacgatatc attgcctgaa ccaacttgcg
      181 aaagatcgat atttttgcca acaatgcctg cccggaccgg tgtaccgaat aagaacatcg
      241 gagcaactgt gccatggtcc gtgccttttg agccattttc tttcggtctt cttccgaatt
      301 cagagaaggt cactcctacg accctgtcgt caagctgcat catcctcagg tcgttttgga
      361 aagcaaggat ggactcagaa agctgagtaa ggagcttgcc atgtgtgcca tcaagcggat
      421 tggatccctc tacctgattt tgatgggtat cgaagctgcc gatagacacc acataaatct
      481 tggtcttccc tccgccacta atcatccttg ccactgtctt gagctgatct gccaaatcgt
      541 aatcaggata agtacccata ttgataccct tcttgaatac ttcggatata cggttggagt
      601 agcgacttgt agaatgttct atatcgatga tgtactcaat attattgcca taatcagaag
      661 tcggaatatg tgcaggtgca ataccgccta actcggaaac caatgaatag aaaccggccg
      721 gatcctgccc tgaaaggttg attccggctt cgtgctggtg ttctgtatga aaacccagaa
      781 actgttgttt acttcctagc tgtatcccta atggatcagg atgatctact gtcggttctc
      841 ctgccagtcc gggataggat gcatcgagat accggcccat ccatccgctt ttgtaattga
      901 agtgagccgg tgtgctgtcc cctcctgata gccaaaggtc tgttcccttg aaatgtgatt
      961 tgtttgaatt ggcataacct acagcctgaa caagggtaaa acaaccctgg tcgtagagtt
     1021 ccttaaagga aatcaggccc ggatgaaggc ttatgtggtc atcattggat aatgtattgt
     1081 ccaggggaat caattggtct aaagaggttc caatggtagg tctaaagttt ttatacgtgt
     1141 catattgatt agttggtaca atacagttga ggccatcatt gcctccctta agctggacaa
     1201 tcatcaggat ccggtcattg acatcctcac aattgaagat gcgggcaagt gatcctgttg
     1261 caaatggctt taggatactt ttttcaagaa ctaaaggacc taagactgca ggtgttgata
     1321 ttcgaaggaa attacgtctt ttcatgatta ttgatttatg ccagaataat aggattataa
     1381 caattggtat tcctgggaat acatgattgc cgtaaggagt ttggacaatc cgatcttaac
     1441 attttcagga tcgccgctgc ttgtataggc cacccattcg taggtccaat cggcaggcgg
     1501 tattccatcg agcatttcat tgaggaaata attgattcgg tcatcatccg gtggttcggg
     1561 caacatgcca ttggccattt ccttcgttaa ctgtataggg tctcctggat tggagaaaaa
     1621 gcctgaattc cggacaaagg caggtaaatc cagggttacc ttggtgctct tgcctccaag
     1681 ataggatgtc ccggttaaca acatttcaca tagtttgtac ctagaaacga tagtattggc
     1741 agtaaaccag gattcatcat acacaggttc ctgataataa gctgaatagc ctgccacatc
     1801 gtatggagcg aaaaaaggca gtgcaccatt gtccagaatc acagctattg ctaatctatt
     1861 gataaagttg tacaaactgg ctgtttctgt agtctgatcc ggccattttt gatggaggaa
     1921 gtttacactg ccaagaaaga gttccagagg gctttttatc aaagcaccga ttatttcatc
     1981 cttgttgtct gaatcatcct catcataaaa atggacagaa gttagtaagg tctttattgc
     2041 tgaggctgtc tcataattat tatccatcaa ttgctgcgcc aatggatgga tgatatcgga
     2101 ttcaatttca ggggtgatat tggatgaaat gaaatacctg tataagcgac gacaatatag
     2161 tcgtgccgtc tctatcttgc tgaagaccat gtccacatat tcttcaagtt ctcttggcat
     2221 ttcatccact gatttagcgc ctttgatggt gcggttgtcg aaggctgcac tgaacgtttt
     2281 atcctcggtg ttgtgttggc cgaaagcaaa tcgcccggtt gacaatccgg tgtcggggtc
     2341 gatggtggcc agccttgtat tggctctgat tccggtcaga agtttggctc ccacaacgac
     2401 atcttcttct gtataattgg tgtaatcgcc gggacctacc tgtggtcctt tcccaatggt
     2461 aaacagctcc agaaactctc tggcatagtt ctcattgggg gcattcttat tgttatttgt
     2521 attgttcaga tagatgagca taagattgtc catcgtcatc ttccttgcta aaaccttaaa
     2581 gctacctgtc gtgaatttgt acaataatgc cagatagtcg aaaaatcttt ccgacgtact
     2641 ttgattatag gttgtcacca ggcatttatg tagaaaaaag accatcttgc catgaatggt
     2701 ttcatcaaat ctcatctcgt tgagcaccca ggatcgtaca tagttgcgga gggcgggttg
     2761 tgctgtggtc gtacttgtac cattgttgat ccatggtgct ccggtggctg gatcgatggg
     2821 ttggtccaga gtaaatgtga ccggcaacat cagtttgtcc actgcctctg atgcagtata
     2881 ttgggcaaat tcctttatcc tgccgatatc ataacggtag gtagcccgtc ttagaagatg
     2941 acctgcgagc cgctctccca ggatgccttt tcttaattcc aaagaagcca taataagatt
     3001 ttatctgtta acaaattgat gcttgaaggg tgaaaatttc cctgtcaaca attgatttga
     3061 tatgctaaaa gtgaaagagt ttaatgacca ggtaaatatt tttcttttgc gttatagcaa
     3121 cggtaaaatt aaaatactat tcctgtaata tcaaatattt tgaaatatta tttctcttga
     3181 aattgaaatg gaaaatatat atctttgcga cttgtttttt cttcaaacca aaatttaact
     3241 tctcaaaaag tatgaaaaaa atctttttac aagtatttat ctgtttgatg tgcttttctg
     3301 caatcaatgc acagatcaat tattctgatg atttcgaggc atatgctgcc ggtacgacaa
     3361 ttgtcactaa caaccctaca aactggcgta cctggggatc tacagcaggt ggtgctgctg
     3421 acgatgcata catcaccgac cagtttgctg cgagcggtaa gaacagtatt aagattaaag
     3481 ctaccagtga taatggaggt ccggaggata taatcctgaa gatcggaaat aatatgaaag
     3541 caggaacaat tgatgtctcc tggaatatgc tggtggctga aggtaaggtc ggttatttca
     3601 atttccagac cactcccaat ccgggtacag gatgggcaat ggaattcttt atgaataaag
     3661 acggtagtat tgatgtcgtc actaactcca agccattttc caaccgtctt agttataatc
     3721 cgggcaagtg gtttaacttc agagtagtga ttgatgcttc caataattta tggcaggtat
     3781 atatcgatgg tgaatgtaag ggtgttttca ggaataatct gacaactgtc tcatctattg
     3841 actttttcgc cattgccaac ggagaatact atatcgatga tgtggtactc aattctgatg
     3901 ctaatgacgt gaaggtatat agtggggtag aggcaggtat tatgaacctg aatataacag
     3961 agggtatcca gatcgcaggc accaatatca aaacaagtgt tcaattatat aatgtaggag
     4021 cagatacaat cacaagcatt gaatatagca taaagaatgg cggtgatgtg atcgaagaga
     4081 agttggaagg gctttctctg gctcctaata agtctcagaa tattgcaatc cctacagcga
     4141 tcaagctggc agacggggat aatgccttaa tacttacatt gaccaaaatc aatgggaaag
     4201 aggatgagtt gtcatgtaat aatatactta atgcgtcagc ttatgcagtc caaccggcca
     4261 aaaacagaaa ggttctgtta gaggaaggga caggtacatg gtgtggttgg tgcccaagag
     4321 gtgctgtatt tctgtcacag atatatccaa gctatgacgg atatctggtt ccgatcgctg
     4381 ttcacaatgg tgatcctatg acggtaagca cctatgacaa agaggttact tctttcaatg
     4441 gatttaccgg attcccaggg atgatagttg atcgtcgtat tgtggtggat ccttctgccg
     4501 ctttggcacc gggacttggt taccttaggg aagaaccgga tgcaacacta ctgattggtg
     4561 caacagagcc tgaaggtgac ggaagtatga aagtttcggt aagtctggac tacctgaatg
     4621 aggtgccaaa aggctacagt gtcgtcctta ccatcgttga ggatcaggtc aaagggagta
     4681 ctgcacaata taatcaggcc aattattatt ccggtggtgg aagcggtcag atgggcggtt
     4741 acgaaaatct tcccaatccg gttcctgcag ctcagatggt ttatgagcat gtggctcgta
     4801 cacacgacca ctctgtgaag aatatggatg cctatccggc aggagaaaag gccgttctca
     4861 attacaccgt aaagctcaat tccgactgga atcaggatga gattaaaatc gttgccttcc
     4921 tgcttaacac tgccaaccag gtttccaatg tagatggagt tacactcaaa gatgcttata
     4981 acaacggctt cgtgtcatca actaaggaaa aaattctggt caacagtggt ctgaccgtgt
     5041 atccgaatcc tgtacaagga actactacaa tcgctattaa ccttgacaag ccgactgatg
     5101 tcactactaa catcctggat gcaatgggac gaattgtttt aacgaagaac tacggcaagc
     5161 agaatggtca tgcagttatg aatcttgatg tcaacaatct tgctccgggt tcttatactg
     5221 tgctggtacg taccactgat ggcattgcta cgcataagat ggttgttcaa taaattatac
     5281 attcgttgag ggcaataatg ccttgacggg tgaaaggaaa tcctttgata gggccatggt
     5341 aagatgccat ggctcttttt tttgaattat gattagagaa agcatgtatc gtataggcgt
     5401 tgtcagaagg tacaaatttt tttgttggtg ccgtggttga ttttcaatat tttattgcaa
     5461 taatgtctaa tttttcaaaa aaaatatgga tgcgacttgt gttttccata tatttaccga
     5521 tcaaaaatat aaataacgaa aaatgtcgaa ttatttaaca cgtcggaaat cattgacctc
     5581 cttattatcg gatgccagtg aaagcgaaaa aggtttaaaa agaaccttat cctccacttc
     5641 attggttgcg ttgggtattg gtgccatcat cggtgcagga ttgttttccc ttaccggtat
     5701 tgcggcagca gaaaatgccg gtcctgcagt gaccatatcc tttgtcctag caggtatagg
     5761 gtgtgctttt gccggattat gttatgctga atttgccagt atgatccctg ttgcaggaag
     5821 cgcttatac
//
LOCUS       DAOQQN010000198         5790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6062_length_5790_cov_5.759372, whole genome shotgun sequence.
ACCESSION   DAOQQN010000198 DAOQQN010000000
VERSION     DAOQQN010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6062_length_5790_cov_5.759372"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PLG24_08165"
     CDS             <1..883
                     /locus_tag="PLG24_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ24904.1"
                     /translation="LLAGWVLLCTIYYFIFMQERLEHEIIKKIDVDMDRCLTHRLEPA
                     PLEIVDETGGYSIKLRGNIHCKWKSAKPEFYCRDQFRSNFTNLKHYLEDHPDKALRIN
                     GNYSKEEYNQTELGDLGYTRAEVLRQYLIDSMGYRIEMINIGTGVKETNDLIVLDKML
                     YRPYVLTIEDIITFTANSDLEYVQPIYFDFDFRSLIPSDSLLNYLGNIASKYKNKKSY
                     LLTITGHTDYMGSNEYNMKLGMDRAKFIEALIQEKFNYQNTVCRSEGEENPKYDNSTS
                     QGRRMNRRVDLQIEKTE"
     gene            1023..3317
                     /locus_tag="PLG24_08170"
     CDS             1023..3317
                     /locus_tag="PLG24_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24905.1"
                     /translation="MNRQTHKYPLILICLFLWVTLDAQCIKDAFVRIDTIELPAEIKN
                     RLQIKITRFYQAYKAQSRVSDFTIVENYHGTDRYLKIMDIAQVRDTMNVLVHIDHISD
                     QNNINLIIDAFNRLNQSEKSTVFLPTIIGDSLNFYKIKKFNSMNNVLAVNDFKQKRYS
                     LDQRAIRGNLNTLHSTSCYIWLIDSKNIYNIHATIGKIFPNENKNKQAAPLFVPYLIS
                     DIPTSDYSQLRDIMQKVPCSIDSVIIDINLKKFHRHLKDIKSANFPVSYDVFFDPAKP
                     FYLGENRQIVLTWRSNLGKDLIVLRDTATYSFGSSDFPVNVKELKKDSLFWFSSAFIG
                     ILFLLVLFFILSILYPKIEERKFFKEYVSPYKPVNNIIKLDNITNEPIKEGTLVVQRC
                     KEVVPFYIWKSLGNQCPSYPECMDILGCDGCGKSDINYKFFSKSGSLKKYNWLFFGAC
                     GGFFAWVFTLLTIPVFHRIHLADLFQGLYEKKELINSSLMFFKENVINQSAVGFGIAL
                     GLAGVLTMVDYFSSIVRQHIIINILKIVDLAIVAALFFGLVNAFSYRFDITPFFSGLI
                     SWTGFALLFAITLSLKSATIRFKRALIASLMGVLITYLFFYISSSIVNIGYENTTIFR
                     QIIMESLKILRFQILGMLIGYITMNVISRQEDYELELQSPDSVRGKVIPLTKALNTNN
                     AITIGTSPKNVIRIKWIDAGAEEYHASINYSAEGPVLETFAEVIVNREYLPKGTRYIL
                     QDRDMIQLGRHSSTLFLFIKKYHS"
     gene            3365..4858
                     /locus_tag="PLG24_08175"
     CDS             3365..4858
                     /locus_tag="PLG24_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24906.1"
                     /translation="MLVLTSLSFQTVSPDRISVRNLGISNKEEVEIFLNFSKDDIVSF
                     NFKSSDEQNVSDKSMTYSLSKIPISLDNSVVREKQVSEASKPVKILSDGQYLLTVRTS
                     QKGVQFFNLNLDKMNKPWNSNAPLIKIHASIMDRPGRNQKKLRLSYSVQEKSSINIIG
                     AGKSADIFTLEENSFRINTTLDKGINISEKSKPEILIYLDEENLKKKNLWQIVESLFK
                     KGRYPNLKNLAEISFGVVQNSTAESSKDDLVFSKPSSNQTGTDQKGQGGQSRGSQSGT
                     GNSFAGTNNSGMPLSRIPQDTANLFDTPEKITIGSNFQRIDSSMYLSPVFDYSADQKR
                     KCKSISKMIGVGEDDMLGFWVGISTRQLLEYNRQREVYKGKMIGYWSPLMQYADVLYI
                     RKMMPMAMNIETRHLPLYAFTTMLDNIEIAIVDEQNKELFEKQGDYKSYIDSPYNGLY
                     KTSGQMFGFIKLPDESTELYLCSCNRNKYNGVRLYTIFETFSKNELQ"
     gene            4855..>5790
                     /locus_tag="PLG24_08180"
     CDS             4855..>5790
                     /locus_tag="PLG24_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24907.1"
                     /translation="MTIIKSYRLIILISITALVSSQLNAQSFFNRSWAKKFKNLFSSS
                     EKLPEPDIYVLKKQSLVMVDEPIWLIKNKSYEKFYTNLMTSVVIGNYDINLLNGEARN
                     DEGTTWFDDGFSGVRDSLSNVYSKLLEKNKHLEFYLLVTSYGDIIDQSYLSSIYNSFL
                     NEKESQNKMIDNLRTIIRNLQLKLKIQEHQIGIIFDIQNIPKDGIQSEKVVNFYTYIK
                     EQLFPKGSLSKIGLKLPNLVEENSIYYDGNTLGKIGPLFDFVIYKNYCLDFEKVASPE
                     YLEALTSHNTANLRKGIDSLSRMGINKHDIIIEFAY"
BASE COUNT         1878 a         1102 c         1121 g         1689 t
ORIGIN      
        1 actattggca gggtgggttt tactatgcac gatttattat tttatcttca tgcaggaaag
       61 actggagcat gaaattatta aaaaaatcga tgtggatatg gaccgatgcc tcactcacag
      121 acttgaaccg gcccctttgg agatcgttga tgagactggc gggtattcca ttaagctgag
      181 agggaacatc cattgtaagt ggaaatctgc caagccggaa ttttattgca gggatcaatt
      241 cagaagcaac tttaccaatc tgaaacatta tcttgaggat catccggaca aagcattgag
      301 gatcaatggc aattactcaa aggaggagta caatcaaact gaattaggcg atttaggcta
      361 tacaagagcc gaagtactaa ggcagtattt gattgattca atgggatatc gaatagaaat
      421 gatcaatatt ggtactgggg tcaaggaaac aaatgattta attgtactgg acaaaatgct
      481 gtacagaccc tatgtactta ccattgaaga cataataacc ttcaccgcca attctgattt
      541 agagtatgtg cagcctatct attttgattt tgattttcga tccctgatcc catcagattc
      601 cttacttaat tatttaggca atattgccag taaatataaa aataaaaaat cctacctatt
      661 gaccattact ggtcataccg actatatggg cagtaatgaa tacaatatga aactcggcat
      721 ggatagggct aaatttattg aagccttaat acaggaaaaa ttcaactacc aaaacactgt
      781 ctgcaggagt gagggcgaag aaaaccctaa atatgacaac agtacaagtc aaggccgcag
      841 gatgaacaga agggtagatt tacaaattga aaaaacggaa taaaaatgat tacatattta
      901 gaattgctga ttatgcttct gggcactacc attattggac tttttgccgg aatttacatt
      961 ggaaaggtaa aatatgggaa aagtcaggac aattttataa aaatgcatag aagaaaagta
     1021 ggatgaatcg tcaaacccac aaatatcctt taatcctgat atgccttttt ctatgggtaa
     1081 ccctggacgc ccagtgcatt aaggatgcat ttgtcaggat tgatacaata gaattacccg
     1141 ctgaaattaa aaatcgcctt caaataaaaa ttacaagatt ctatcaggcc tataaggcac
     1201 aaagcagggt aagcgatttt accattgtgg aaaactatca tggtacggat cggtatctca
     1261 agatcatgga tattgctcaa gtgcgtgata caatgaatgt ccttgtgcac atcgaccata
     1321 taagcgacca gaataatatt aacctaataa tagatgcctt caatagatta aatcaatcgg
     1381 aaaaatctac tgtcttttta cctaccatca taggtgatag tctgaatttc tacaagatta
     1441 aaaaattcaa ttccatgaac aatgttctgg cagtgaatga ctttaaacag aaacgatact
     1501 cacttgacca aagggcaata agaggcaacc taaacacttt gcattctact tcctgctata
     1561 tatggctcat tgattcaaag aatatttaca atatacacgc tactatcgga aaaatttttc
     1621 caaacgaaaa caagaacaaa caagcggcac ccctttttgt cccttatctt atttccgaca
     1681 ttccgacttc ggattattct caattgaggg acattatgca gaaggtgcca tgctcgattg
     1741 atagtgtcat catcgatatt aatctgaaaa aattccatag acatctaaaa gatatcaaat
     1801 ccgcgaattt cccggtctcc tatgatgttt tctttgaccc agcaaaacct ttttatttgg
     1861 gtgaaaacag acagatcgta ctaacctgga gatcaaattt ggggaaagac ctgattgtcc
     1921 tccgggatac tgccacttac tcatttggca gcagcgattt tccggtcaat gtcaaggaat
     1981 tgaaaaaaga ttcgcttttc tggttttcaa gtgcatttat tggcattctg tttttattgg
     2041 tactgttctt catactcagt atactttatc ctaagattga agaaaggaaa ttttttaaag
     2101 aatatgtttc accctataag ccggtgaata acatcatcaa actggacaat ataactaatg
     2161 aaccaataaa agagggtact ctcgtggttc agaggtgtaa ggaagtggtg cctttttata
     2221 tttggaaaag cctcggcaat caatgccctt cctatcctga atgtatggac attctggggt
     2281 gtgatggttg tggcaagtct gatatcaatt ataaattctt ctccaagtca ggcagtttga
     2341 aaaagtataa ctggcttttc tttggagcct gtggtggttt tttcgcctgg gtatttacct
     2401 tgctcaccat acctgtgttt caccggattc atttggcaga tctgtttcag ggcttatatg
     2461 aaaaaaagga gcttatcaac tccagtctga tgtttttcaa ggaaaatgtc atcaatcaat
     2521 cggcagtcgg tttcggcatt gctcttggcc tggcaggtgt attaaccatg gtggattact
     2581 tttccagtat tgtgagacaa cacataatta tcaacatact caaaattgtc gaccttgcca
     2641 tagtagctgc attgttcttt ggattggtca atgcattttc ctaccgattc gacatcacgc
     2701 ctttcttttc cggccttatt agctggaccg gatttgcttt gttatttgcc atcacccttt
     2761 cgctgaaaag tgcaacaatc agattcaaaa gagctttgat cgcttccttg atgggtgttc
     2821 ttataacata ccttttcttt tatatcagct cctcgattgt caacataggg tatgaaaaca
     2881 caactatatt tcgtcagatc attatggaat ctctaaaaat tctcagattc caaatacttg
     2941 gcatgttgat tgggtatatc acgatgaatg taatatcaag gcaggaagat tatgaattgg
     3001 agctacagtc gcctgactcg gtaaggggta aagttatccc gctgacgaaa gcactgaata
     3061 ccaacaatgc catcactatc ggaacttctc caaaaaatgt catcagaatc aagtggattg
     3121 atgcaggtgc tgaagaatat catgcttcca tcaattattc ggcagaaggg cctgtacttg
     3181 agacctttgc tgaggtgata gtcaacaggg aatatctacc taaaggcacc cgctatattt
     3241 tacaggacag agatatgatt caattgggtc gacatagttc aacgctattc ctttttatta
     3301 aaaaatatca ttcatgatgt tcctttacac acaatcgttt tacgggaggt taataatttt
     3361 aaccatgctt gtgcttacat cactgtcatt tcaaactgtg agtccagaca ggatttcggt
     3421 caggaatctg ggcatctcca acaaggagga agtcgaaata tttctaaact tcagcaagga
     3481 cgatattgtc tcgttcaatt ttaaatcatc agatgaacaa aatgtgtcgg ataaaagcat
     3541 gacctattct ctcagcaaaa ttcctatatc tctggacaat tcggttgtca gagaaaaaca
     3601 agtatctgag gcctccaaac cagtcaagat attatctgac ggtcaatatt tattaactgt
     3661 ccggactagt cagaagggtg ttcaattttt caatctgaat cttgataaaa tgaataagcc
     3721 ctggaatagc aatgctcctt tgattaagat tcatgcatcc atcatggaca gaccgggcag
     3781 aaatcaaaag aaactaaggt tatcctattc agtacaggag aagtcttcca tcaatatcat
     3841 aggtgcaggc aagtccgcag atatcttcac actggaagag aattctttta gaatcaatac
     3901 aaccctggat aaaggaatca acatctctga aaagtcaaaa ccggagatat tgatttacct
     3961 cgatgaagaa aacctgaaga agaagaatct ttggcaaata gtggaatctt tattcaaaaa
     4021 aggaaggtat cccaatctta agaatctggc agaaatcagc ttcggcgttg tccaaaattc
     4081 cactgctgaa tcttccaagg atgaccttgt atttagcaag ccttcttcaa atcaaacagg
     4141 gacagatcag aaaggacagg gaggacaatc aagaggcagc caatcgggaa ccggcaattc
     4201 atttgcggga accaacaatt ccggtatgcc gttaagcagg attccacaag acactgccaa
     4261 tctcttcgat acaccggaga aaatcactat cggctctaac ttccagagga ttgactcttc
     4321 gatgtatctt tcccctgttt ttgattactc tgctgatcag aaaagaaaat gcaaatctat
     4381 ttcgaaaatg atcggagtag gtgaggatga tatgcttgga ttctgggtag ggatctcaac
     4441 ccgacaatta ttggaatata accgccagcg agaggtctat aaagggaaaa tgatcggcta
     4501 ctggagtcca ttgatgcaat atgccgatgt actatatata aggaaaatga tgcctatggc
     4561 tatgaatatt gagacaagac accttccatt atatgctttt acaacaatgc tggacaatat
     4621 tgagattgca atcgtggatg agcagaacaa agaactcttt gaaaagcagg gcgattacaa
     4681 atcatatata gactctccgt acaatggtct gtacaagact tccggtcaga tgttcgggtt
     4741 tattaaattg ccagatgaaa gtaccgagtt gtatctgtgc tcctgcaata gaaacaagta
     4801 taatggggta aggctttata ccatctttga aaccttttca aaaaatgagc ttcagtgaca
     4861 atcattaaat catatagact aatcattctg atatctatca cagccctggt atcaagtcag
     4921 ctcaatgctc agtctttttt taaccggagc tgggctaaaa aattcaagaa cttgttttca
     4981 agcagtgaaa aattgccgga gccggatatc tatgttctta agaaacaaag tctggtaatg
     5041 gtcgatgagc ctatctggct tattaaaaac aaatcttacg aaaagtttta taccaatctg
     5101 atgaccagtg tagtcatcgg caattatgac atcaaccttc ttaatggcga agcacggaat
     5161 gatgaaggca ccacctggtt tgacgatggc ttttccggcg taagagactc cttgtccaat
     5221 gtgtattcca aattacttga aaaaaacaag catttggaat tttatctcct ggttacctct
     5281 tatggtgata tcatcgatca aagttatcta tccagtattt ataattcttt tttgaatgag
     5341 aaggaaagtc aaaataaaat gattgacaac ctcagaacaa ttatcaggaa ccttcaactt
     5401 aagcttaaga ttcaggagca tcaaataggt ataatctttg atatacagaa tattcctaag
     5461 gatggtattc agtcggaaaa ggttgttaat ttttacacat atattaaaga acagctcttc
     5521 ccaaaaggca gcctttccaa gattggacta aaattgccca atctggttga agaaaattca
     5581 atttattatg acggaaatac ccttgggaag ataggaccgc tttttgattt tgtcatttat
     5641 aaaaattatt gcctcgattt tgaaaaagtg gcatcccccg aatacctgga agctctaacg
     5701 agccacaata ccgccaacct ccgcaaggga atcgactctc tatccaggat gggtattaac
     5761 aaacacgata ttatcatcga atttgcctat
//
LOCUS       DAOQQN010000199         5784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6076_length_5784_cov_6.202479, whole genome shotgun sequence.
ACCESSION   DAOQQN010000199 DAOQQN010000000
VERSION     DAOQQN010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6076_length_5784_cov_6.202479"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLG24_08185"
     CDS             complement(<1..509)
                     /locus_tag="PLG24_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24908.1"
                     /translation="MQKMMFRNFGLLGFLFLLISVANAQDIEEAYKKLSYGKTDEAVN
                     IAKSVANADPAIMANKFSLYSLLNAAERYDEAGKVLSEIKTSDESGPYGKTADVLMKL
                     DQGANPDDLTVEIDKAIRKGKKMKGFLYRTVGEYFLFNKKTNPVKAIQYIKMAIDDYG
                     LKNASTRML"
     gene            complement(522..1463)
                     /locus_tag="PLG24_08190"
     CDS             complement(522..1463)
                     /locus_tag="PLG24_08190"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HMZ24909.1"
                     /translation="MKVKIGSVAGIILSLMIASCNQAPTTQYRDTPTSGEAHIAGDFL
                     YKTMLDSEIQIFTHEYQKASIAVDYMSEALAVKAFLSDSIDGIVIGRELNASETKTFR
                     DKKKYDPKTVRLATDAVAVIVHPDNKAEDLTIDLLRNILNGSTTDWGDGRKIEVLIDN
                     EGSGIITFLRDSVMAGSSFGKNVYTLPQGTTMIDYVATHPNAMGFVGVNQITSFDGDA
                     NQVFNEKIKPLAIGKVAGLKSYKPYQAYVASRNYPLLRFTNFIFNESHNGLTSGFATF
                     MASDKGQRIILKDGLVPANAPIRLIQVNTSANDINSR"
     gene            complement(1465..1746)
                     /locus_tag="PLG24_08195"
     CDS             complement(1465..1746)
                     /locus_tag="PLG24_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24910.1"
                     /translation="MEFFPVAILFLRLIDKLTKPGNQINQSFQLLATVLALIIGIFFT
                     GFGISILTGLIKMPNDDGPIMKIFGGLLVIYGIFRLIRVYFKYKSYREE"
     gene            complement(1782..2627)
                     /locus_tag="PLG24_08200"
     CDS             complement(1782..2627)
                     /locus_tag="PLG24_08200"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HMZ24911.1"
                     /translation="MKSEDILKADMLDIIFDGRNKEYGAYDLRRKYPKFLGRALIICI
                     LIVSAILGYALLKERFAGIFEKEVQVEANLADLEAPPPMEENEPPPPPETAPPPPPER
                     ASVQYVPPVVKEVVAQEAEIATVEELKDKDPGVKTVEGDPNAKVIIDTKADAAPGLFE
                     SSEKKVVAKVEDDNKIFTVVEQQPEFPGGIGAMYKWIGENLKYPSEARNNGLQGKVIL
                     QFTVEKDGSIANVKVVRDGVGGGAGDEAVRVVKKMPAWKPGKQNGKSVRVQYTLPVTF
                     KLEGE"
     gene            complement(2702..3271)
                     /locus_tag="PLG24_08205"
     CDS             complement(2702..3271)
                     /locus_tag="PLG24_08205"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMZ24912.1"
                     /translation="MAQMDTASSGGGNKKGGSKTKKLSTRVDLTPMVDLAFLLITFFM
                     LTTTMNKPQAMQINMPVERNPNDPPPPEVPAAKVLTLILGANNVIWYYEGLDSPEIKN
                     TDYTEIRNIILDKKKRVEATIKPDDKIKSVIVIIKPTDDSNYKNVIDMLDEMAIDEIG
                     TYTMVPVTPQELALVDKFKNGGGTPPPAN"
     gene            complement(3304..3948)
                     /locus_tag="PLG24_08210"
     CDS             complement(3304..3948)
                     /locus_tag="PLG24_08210"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMZ24913.1"
                     /translation="MPKHKIARKSTAVDMTAMCDVSFLLLTFFMLATKFKPPDPVEVV
                     TPASISDTKVAETNLIMITIDKNGRVFFDMDGIPTKMDLINQINDNMRLGLTEEEKKN
                     FALSSGVGVPFSQLKALLKHSPTEQKSFNSTGIPVDTSLTDSNELFKWVAYGRLSNPE
                     ARLSVKGDGSVKYPVVKAVFSTLANKKIQGNKFYAVTSLKANPNKPVEEPEKTN"
     gene            complement(4003..4818)
                     /locus_tag="PLG24_08215"
     CDS             complement(4003..4818)
                     /locus_tag="PLG24_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HMZ24914.1"
                     /translation="MANAPKQNEKKTNSGSILPYIAIPLAFVIGIILYKFVFGAASHF
                     KNGDPNNDPLPGDYMGVIYKGGFIVPILLGLLFITIIFSIERFITIMRAAGTGPVDKF
                     VRNVQYSLSKGDINGAIAACDKQKGSVGNVVRSGLTTYNKMTSESGMTKEQRIAAIQK
                     EVEEATSLELPSLEQNLPIIATIATIATLIGLIGTVLGMIRAFAGMATSGAPDASALA
                     TGISEALINTALGITTSAIAIVAYSYFTTRIDGMTYRIDEAGYSMQQQFSSQY"
     gene            complement(5543..>5784)
                     /locus_tag="PLG24_08220"
     CDS             complement(5543..>5784)
                     /locus_tag="PLG24_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ24915.1"
                     /translation="DRGVKLYPNPASERLTILLIGETETVAKYSILSMSGRNQMQGIL
                     PDKQSTIDVSGLAQGMYCLTIDMPGKTITTKLIKL"
BASE COUNT         1586 a         1229 c         1092 g         1877 t
ORIGIN      
        1 aaaagcattc tggttgatgc attcttcaat ccataatcat caatggccat cttgatgtat
       61 tgaattgcct tcaccggatt ggtttttttg ttaaaaagaa aatattcgcc gacagtacga
      121 taaaggaatc ccttcatctt cttacccttt ctgattgcct tgtcgatctc aacagtcagg
      181 tcatcaggat tggctccttg atccaatttc attaagacat cggcagtctt accgtaaggg
      241 ccactttcat cacttgtttt aatttctgaa agcaccttgc cggcttcatc atatctctct
      301 gctgcattca gaagactata taaggaaaat ttatttgcca taatagccgg gtcagcattg
      361 gcgactgatt tagcaatatt caccgcctca tcagttttgc cataagatag ttttttatag
      421 gcctcctcga tgtcctgagc attcgctaca cttattaaca ggaataggaa gccaagcaaa
      481 ccaaaatttc taaacatcat tttttgcatt gttaattgat tttatctgga attaatatca
      541 ttagccgaag tatttacctg aatcagacgt attggcgcat tagcaggtac cagtccatcc
      601 ttaaggataa tacgttgccc cttatcggat gccataaagg tagcaaatcc actggttagt
      661 ccattgtgtg attcattgaa aataaaatta gtaaatctca acaaggggta atttcgggat
      721 gccacatagg cctggtatgg tttatagctc tttagaccgg cgactttacc aatagcaagt
      781 ggttttatct tttcgttgaa aacctgattg gcatcaccat cgaaactggt gatttggttg
      841 acaccgacaa aacccatagc attaggatga gttgccacat aatcgatcat ggtggtacct
      901 tggggcaaag tatatacatt tttaccgaag gagcttccgg ccattaccga atctctcaga
      961 aaagtaatga taccactccc ttcattatct atcaatactt ctatcttacg gccgtcaccc
     1021 caatcggttg tagaaccatt caatatattt ctaaggaggt cgatggtgag atcttcagcc
     1081 ttattgtccg gatggacgat caccgctaca gcatcagtgg caagacggac agtcttggga
     1141 tcatattttt tcttatctct gaatgttttt gtctcagaag cattcaattc ccttcctatt
     1201 acaattccat cgatactgtc actcaggaag gctttgactg ctagagcttc agacatatag
     1261 tcaacagcaa tggatgcttt ttggtattca tgggtaaaaa tttgaatttc actatctagc
     1321 atggtcttat acaggaagtc accggcaata tgtgcttctc ctgaggtagg ggtgtctctg
     1381 tattgcgtgg ttggtgcctg attacatgat gcaatcatta gcgaaaggat gattccggcc
     1441 actgaaccaa tttttacctt catgttactc ttcccgatag gatttatatt tgaaatatac
     1501 cctgatcaac ctgaaaatcc cgtaaatcac cagcagacct ccgaatatct tcatgattgg
     1561 accgtcatcg ttaggcatct tgattaaacc ggtcaggatg cttataccga aaccggtaaa
     1621 gaatatgccg attatcaatg ccagcacggt ggccaatagc tgaaaagatt gattgatctg
     1681 atttcctggt ttagtcaatt tgtcaattaa tcgtaaaaac aaaatagcaa cagggaagaa
     1741 ttccatattg ctattttctt ataaaaattt taacgcgaat gttattctcc ctccaacttg
     1801 aaagtcaccg gtaacgtata ttgtacgcga acagacttac cattttgttt tcctggtttc
     1861 caggctggca tttttttcac taccctgaca gcttcatcgc cggcaccgcc acctacgcca
     1921 tcacgtacaa cttttacatt ggctatggaa ccgtcttttt caactgtaaa ctggagtata
     1981 accttacctt gcaaaccgtt attacgtgcc tctgatgggt acttcaggtt ttctccgatc
     2041 cacttgtaca tagctccgat cccacctggg aattccggtt gttgttccac cacggtgaag
     2101 attttgttat catcttctac tttagctaca accttttttt cggaagattc gaataatccg
     2161 ggtgcagcat cagcctttgt gtcaataatc actttggcat tcgggtcacc ctcaactgtt
     2221 tttacaccgg ggtccttgtc cttcaattct tcgactgtgg ctatttccgc ttcctgagca
     2281 actacctctt taacgaccgg tggcacatac tgaaccgaag ctctttccgg tggtggagga
     2341 ggtgcagttt ccggaggtgg gggtggctca ttttcttcca taggaggagg tgcttcaaga
     2401 tcagccagat tggcctctac ttgtacctct ttttcaaata ttcctgcaaa cctttctttc
     2461 aaaagggcat atcctaaaat tgcagaaaca atcaatatgc agatgatgag ggcccgaccc
     2521 aaaaacttag gatactttcg cctcagatca taagctccat attctttatt tcgaccatca
     2581 aaaatgatgt cgagcatatc cgcttttaaa atatcttcac ttttcatgac gcctttttat
     2641 ttgtataacg caaaattaag ttatataatt tcattttaca actatttttt acgttatatt
     2701 tttaattagc cggaggcggc gtgccacctc catttttaaa tttatccacc agggccaact
     2761 cttgaggtgt cacaggtacc atcgtataag tacctatttc atcaatcgcc atttcatcca
     2821 gcatatcgat tacattttta taattggagt catcagttgg tttgatgatt acaattacag
     2881 atttgatttt gtcatccggt tttatcgtag cctccactct tttctttttg tccagaataa
     2941 tatttctgat ttcagtataa tcagtatttt ttatttccgg actatccagt ccctcatagt
     3001 accaaatgac attatttgct cccagtatca atgtaagaac cttagctgca ggtacttccg
     3061 ggggtggggg atcattggga ttcctttcca ccggcatatt gatttgcata gcctgaggtt
     3121 tattcattgt tgtggtgagc atgaagaatg taatcaatag gaaagccagg tccaccattg
     3181 gcgtaaggtc aacgcgggtt gataactttt tggttttact gccgcctttc ttatttcctc
     3241 cgccggaaga ggcggtatcc atttgtgcca taacaatttg gttattttac acagattaat
     3301 aatttaattt gttttttcag gctcttcaac cggcttatta ggattggctt tcaggctggt
     3361 aacagcataa aacttatttc cttgaatttt tttgttagcc agggtagaaa atacagcctt
     3421 tacaaccgga tacttgaccg aaccatcacc tttcacggag agtcttgctt ccggattaga
     3481 taaccttcca tatgcgaccc atttgaataa ttcattggag tccgttagac ttgtatcgac
     3541 cggaatcccg gtggaattga atgatttctg ctcggttggc gaatgcttta acaatgcttt
     3601 caactgacta aaaggcactc caacaccaga actcaaggca aagtttttct tttcctcttc
     3661 ggtcagacca agcctcatat tgtcattaat ctgattgatc aaatccattt tcgtcggaat
     3721 accatccata tcgaaaaaga cccttccatt cttatctatg gtaatcatga taagattagt
     3781 ctccgccacc ttggtatctg aaatagaagc gggggtgaca acctcaacag gatccggtgg
     3841 cttgaatttg gtcgccaaca taaagaatgt caaaagcaga aaggatacat cgcacatggc
     3901 cgtcatgtcc actgcggtac tttttcttgc gattttatgc ttaggcatta tttaattttt
     3961 tggggttaaa gaattgtcca tcaaaccgga accaatacgc tatcaatact gagagctgaa
     4021 ttgctgttgc atactatagc cggcttcgtc gatgcgatac gtcatgccat caattcttgt
     4081 tgtgaagtag ctataggcaa ctatcgcaat ggccgatgtg gtaatcccca atgcagtatt
     4141 gatcaatgcc tcagaaatac cggtagcaag tgctgatgca tccggagctc cactggttgc
     4201 catacctgca aaggctcgaa tcatacccaa taccgtaccg atcagaccga tcaaggttgc
     4261 aatggttgca atggttgcaa tgatgggaag gttctgctca aggctgggga gctcaagaga
     4321 agtggcctct tcgacctctt tctggatagc ggcaattcgt tgttccttag tcatacccga
     4381 ctcacttgtc attttgttgt acgtggtaag cccggagcgc accacatttc ctacagaacc
     4441 cttttgctta tcacaagcag ctatggctcc attgatatcc ccttttgaaa gagaatattg
     4501 tacatttcga acaaacttgt ctacagggcc cgtaccggct gcccgcataa tcgtaataaa
     4561 ccgctcaata gagaaaataa tagtaataaa taataatccg agcagaatag gcacgatgaa
     4621 tccaccttta tatataacac ccatgtaatc accgggtagt ggatcgttgt tggggtcgcc
     4681 attcttaaaa tggctggctg cgccaaatac gaatttgtac aaaataattc caatcacaaa
     4741 agccaacgga atagcaatgt agggcagtat agaaccggaa ttggttttct tttcgttctg
     4801 cttaggtgcg tttgccatgt taaatctttt ttaattttta caatgatatt ttaaaactta
     4861 gaatgagaag caaatatatt tttttgatta caactaaaca aaatattttt tgtaaaataa
     4921 aaatcattga aatggaatat tttgataaaa cgccccaaaa catataacta tcaataaatt
     4981 ataagttatt taaaaactta ttgtttgttt ataaattttt atttgcccga tataataata
     5041 atatttcttg gtcctttatt gcgttttcat tcatctttta catcttcatt tagaatattt
     5101 tgaaaaaccg ttagctcctt tttgcaaccg gagccatttt tccggttttt ttagaatcat
     5161 acaggatctt atgttgattg taaagctcaa tgaccatcct gtacatttcg ttttacaagg
     5221 attggatttt taaactgcag ataatcaata ccatatgttt attgtctcac ctgctgtaca
     5281 agatttcctt atttaaaatc aatgtttgat aattgatctc gctgacaatt tataagatta
     5341 ttatctgttg ttgtcctatg tggtcaaact caaaatttta cattcttata ttatctgtga
     5401 ttgagtcgga catttcaaaa ttcaggtcag gttaaacaac aaggaccatc ggcttatact
     5461 tcactccatt tagcaagaat gctccggaag ctaaatgatg cctcgatgag caaagtattt
     5521 tgggcaagtg acattctatt cactatagtt tgatcagttt ggtagtgatt gttttgcccg
     5581 gcatatcaat agtaagacaa tacattcctt gtgcaagccc ggagacatcg attgttgact
     5641 gtttgtccgg caggatgcct tgcatctggt ttcttccgga catggatagg atggaatatt
     5701 ttgctacggt ttctgtttca ccgatcaaca ggatagtcaa cctttcggat gcaggattgg
     5761 ggtaaagctt gaccccacgg tctt
//
LOCUS       DAOQQN010000200         5779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6082_length_5779_cov_6.137491, whole genome shotgun sequence.
ACCESSION   DAOQQN010000200 DAOQQN010000000
VERSION     DAOQQN010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6082_length_5779_cov_6.137491"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PLG24_08225"
     CDS             <1..822
                     /locus_tag="PLG24_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HMZ24916.1"
                     /translation="AWKEGIRLTLRTFREQQRKKDNGPYKFQRTTAWATDGVPIAGYG
                     YPCKPVGLIHSMFRPSDDATIYPFLIPSNFFAVRSLRQAAEMLSAIHHDNLMAGECIA
                     LADEVEAALKKYAVARHPSFGKIYAFEVNAYGSANLMDDANVPSLLSLPYLDTVSLKD
                     KNYRNTRKYILSNENPFFFKGKAGEGIGGPHTGMDMIWPLGITMRGLTSSDDHEIRFC
                     IRQLLATHGGTGFMHESFHKDDATNFTRKWFAWSNTLFGEFLWKIYREKPALLDV"
     gene            complement(1257..3164)
                     /gene="acs"
                     /locus_tag="PLG24_08230"
     CDS             complement(1257..3164)
                     /gene="acs"
                     /locus_tag="PLG24_08230"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HMZ24917.1"
                     /translation="MGKYKIKNLEKYFKEYRKSIKDPTGFWEKIAAENFDWHSRWNKV
                     LDFDMKEARFEWFRNAKLNITQNCIDRHLQTQGDKTAIIFEPNDVNEPAQHITYRELY
                     ARVAKMANVLTDQGIRKGDRVCIYLPMIPELAVSLLACARIGAVHSVVFAGFSASALA
                     ARINDCDCKTVITSDGSFRGNKTIDLKHIVDEALHQCPGVERVLVVRRTGGDVVMKKG
                     RDQWLQPLLDEAAALHVAEVVDAEDPLFILYTSGSTGKPKGMLHTTAGYMVFMGYTFK
                     NVFGYKKKDVYWCTADIGWVTGHSYIVYGPLLNGATTLIFEGIPSYPDYGRFWQVIEK
                     HKVNIFYTAPTAIRSLAKESLDFVQRQDLSSLKVIGSVGEPINEEAWHWYNENIGHRR
                     CPLVDTWWQTETGGILISPLAFVTPTKPSHATLPLPGIQPVLMDELSNEIHGNQSGGN
                     LCIKYPWPGMARTIYGDHQRYKDTYFSTYPGKYFTGDGALRDETGYYRITGRVDDIII
                     VSGHNLGTAPIEDVINLHDDVAESAIVGFPHDIKGNALYGFVILADEAKGKKYDILTA
                     EINALIAEQIGPIAKLDKIQFVSGLPKTRSGKIMRRILRKIAENDLNNLGDTSTLLNP
                     EVVEEIKDNRI"
     gene            complement(3275..3937)
                     /locus_tag="PLG24_08235"
     CDS             complement(3275..3937)
                     /locus_tag="PLG24_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24918.1"
                     /translation="MIGIRLFIMICWLICIIDTLPAQSESPSSATGQAALLKKGFLIV
                     RLPSFDKQEKYYNAIIGDSLSGKKERERAQAQLHALTTRRDGLIRVLRHTIDSVYRFS
                     EVRYISQRDFNSPDKDSINQSFLTPETFKDPSLSIGDRPYMVLAYMDRTSEAGTNMLN
                     LRLLLADGNEANQAIQFPGVGNIINILLDNGKFDAAEKLLLKRMMKFQNRLQKQYANS
                     VK"
     gene            4326..4871
                     /locus_tag="PLG24_08240"
     CDS             4326..4871
                     /locus_tag="PLG24_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMZ24919.1"
                     /translation="MSIIEFNKKIDNISQYLHSFALNLTKNSEDAKDLYQETTYRALV
                     NKDKFAPDTNLKAWLFTIMKNIFINNYRKKVKANTIMDSTANLYYLNAGGSEHNQGET
                     NIFLKELSDMIDQLDDSLRVPFILHYEGFKYQEIADELELPLGTIKSRIFFARKELKK
                     LIKDRYSNIFDIRLKDKKKAV"
     gene            complement(5317..>5779)
                     /locus_tag="PLG24_08245"
     CDS             complement(5317..>5779)
                     /locus_tag="PLG24_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HMZ24920.1"
                     /translation="LIDGRPFEIFTGLADDEDGILIPRWVNQGLIIKNKEADGTSRYD
                     FQFINGRGYKTTIEGLSYKFNPEYWNYAKLISGALRYSMPIEKVVDLINSLQLDESIN
                     TWKNGVARALKRYIPDGTEARKAKCSNCSSTNLHYQEGCLTCKDCGNSKCG"
BASE COUNT         1605 a         1217 c         1278 g         1679 t
ORIGIN      
        1 gcctggaagg aaggtataag actgacactc aggaccttcc gcgaacaaca gcgtaagaaa
       61 gataacggtc cttataagtt ccagcgaaca acagcctggg cgacggacgg ggtgccgatt
      121 gccggatatg gatatccttg taagccggtc ggcttgatac actccatgtt cagaccgagt
      181 gatgatgcga ctatctaccc tttcctgatt ccctccaatt tttttgcggt tcgttcattg
      241 cgtcaggccg cagagatgtt aagtgccatc catcatgaca atctgatggc cggagagtgt
      301 atcgcgctgg ctgatgaggt ggaagctgca ctgaagaaat acgcagttgc aaggcatccc
      361 tcattcggta agatttatgc attcgaggtt aatgcctacg gatcggccaa tctgatggac
      421 gatgccaatg tgccaagtct gctgtcatta ccctatttgg acactgtcag cctcaaggac
      481 aagaactatc gtaataccag gaaatacatc ctgtccaatg aaaacccatt tttcttcaag
      541 ggtaaggccg gagaaggtat tggcggtccg cataccggga tggatatgat atggccgctg
      601 ggcatcacta tgagaggact cacgagctcc gatgatcatg agatacgatt ttgtatccgt
      661 cagttgctgg caacacatgg tgggacaggt ttcatgcacg aatcgtttca caaggatgat
      721 gctacaaatt ttacccgaaa atggtttgcg tggtccaata ccctgttcgg agaattcctg
      781 tggaagattt atcgggagaa gccggcactg ttggatgtat aagaagagag taggcgccta
      841 cattggcact aattttattt gaattgttgt actgatcttt catgccgggg aaagttcatc
      901 ctgatggaat tttttcaaag attgattaat agcctcctcg catcggcctc gcccgacaat
      961 atggttttgg ggggagttgc aatattatca aagaagggat gtttttcacg gtacccgggt
     1021 tggacggatt ttgaaaacat actatcgaac cccatcgtag taaagaaatt ctcatcgcac
     1081 tgacatatgt aacatgccgc atatctcttc tcagaagttg gcatttattc cagtaactct
     1141 taatgcaccg ggtttttgat tcgcgcctgc aaataatacc gaaggtacag catagctatc
     1201 cggacaagta taccaaaaat ttatggacta ttatataaat catcttggta taactttcag
     1261 atcctgttgt ctttgatttc ctctacgacc tcaggattaa gcaaagtact ggtatctccc
     1321 agattattta agtcattttc tgctattttc cggagaatac ggcgcatgat tttaccgctt
     1381 ctggtcttag gcagccctga tacaaactga attttgtcca atttggcgat tggcccgatc
     1441 tgttcagcta tgagggcatt tatttcagcc gtcaaaatgt catatttctt accttttgct
     1501 tcatcggcca gaatgacaaa gccatatagt gcattgccct tgatgtcatg tgggaagcct
     1561 acaatagcac tttcggcgac atcatcgtga aggttgatga cgtcctcaat cggggctgtg
     1621 cctaagttgt ggcctgaaac gatgatgatg tcatcgactc ttccggtaat tctgtagtaa
     1681 ccggtctcgt cccttagagc accgtcaccg gtgaagtatt ttccgggata agtcgaaaaa
     1741 taagtatcct tatatcgttg atggtctcca tagattgttc tggccatccc cggccatggg
     1801 tattttatac aaagatttcc gccggattga tttccatgga tctcattgct aagttcatcc
     1861 ataaggacag gctggattcc cggaagaggg agtgtagcat gacttggctt agtgggggta
     1921 acgaaggcca ggggactgat caggatgccg ccggtttctg tctgccacca ggtgtccaca
     1981 agagggcaac gtctgtgccc gatgttttca ttgtaccagt gccaggcttc ttcgttgatg
     2041 ggttctccga ccgatccaat caccttcagg ctggataagt cttgacgctg tacaaagtca
     2101 agactttcct tggccagtga tcggatagca gtaggtgccg tataaaaaat attgaccttg
     2161 tgtttttcaa tcacttgcca gaatcttccg tagtccgggt aggatgggat tccctcaaag
     2221 atcaatgttg tcgcaccgtt gagcaaagga ccatatacga tgtagctatg ccctgtcacc
     2281 caaccgatat cggctgtaca ccaatacacg tctttcttct tataaccgaa gacattcttg
     2341 aaggtatagc ccatgaagac catataaccg gctgttgtat gcagcatacc cttgggcttg
     2401 cccgtacttc ctgaggtata aaggataaat agcgggtctt cagcgtccac cacctcagct
     2461 acatgtagag cagcagcctc atccagcaat ggttgcaacc actgatcacg tccttttttc
     2521 atgacgacat cacctccggt ccgcctgacc accaggacac gttctactcc ggggcattga
     2581 tgcagagcct catctacgat atgttttagg tcgatggttt tattaccccg aaaagatcca
     2641 tcggaggtga tgacagtttt gcaatcacaa tcattgatcc tagctgcaag agccgatgca
     2701 gaaaatcccg caaaaacaac agaatgaacc gccccaatcc gggcacaagc cagcaacgaa
     2761 acagccagtt cgggaatcat aggtaggtaa atgcacaccc gatctccctt tctgataccc
     2821 tgatcggtca atacattcgc catttttgca acgcgtgcat acaattccct ataggtgatg
     2881 tgttgcgccg gctcattgac atcattgggt tcgaagatga ttgccgtttt atcgccttgc
     2941 gtctgcaggt gcctgtcgat gcagttctga gtgatgttga gctttgcatt cctaaaccat
     3001 tcaaagcgag cttccttcat atcaaaatca agtaccttat tccaacggct gtgccagtca
     3061 aaattttccg ctgcaatttt ttcccagaat ccggttggat ctttaattga tttccggtat
     3121 tccttgaagt atttctcgag gttttttatt ttatattttc ccatgatcca tgtgcatttg
     3181 aatcataaaa ttaaggaata atccactata atcaaaaggg atcctttttt gacagataaa
     3241 aacagaccag tgattcgaaa ttaatgaccg agttttactt tactgaattg gcatattgtt
     3301 tttgcaacct gttctggaat ttcatcatac gcttgagcag taatttctcg gcagcatcga
     3361 acttgccatt atcaagcagg atgttgatga tattaccaac tccgggaaac tggatggctt
     3421 ggtttgcctc attgccgtct gccaataaaa gcctgagatt cagcatattg gttcctgctt
     3481 cagacgtgcg gtccatatag gcaagcacca tatatggccg gtcaccgatg gaaagcgagg
     3541 ggtctttgaa cgtttcagga gttagaaagg attgattgat actatcctta tcagggctgt
     3601 tgaaatccct ttgagagata taccgcactt ccgaaaatct atatacactg tcaatagtgt
     3661 gtctaagaac cctgattaag ccgtcccttc tggtagttag cgcgtgcaat tgcgcttgtg
     3721 ctctttcgcg ttcctttttt cccgataagc tgtcgccaat gattgcgtta taatattttt
     3781 cttgcttatc aaaggatggt agccttacaa tgagaaagcc tttcttcagc agtgctgcct
     3841 gccctgtggc tgaagaaggg gactcgctct gggccggcaa tgtgtcaata atgcatatca
     3901 accaacatat cattataaaa aggcgaatac cgatcataaa agattttgaa taagaggtaa
     3961 aaataagtga atccgggaag taatttcggt aacagaaaca ttaagttaac aaattatgga
     4021 aactaatgag tagattagaa tcgttctaaa tatgatcctg taaaaagtgg caggggacac
     4081 tgtcaaaatg gctaccatgg taatatggag ccattattgc agtatttagg acaaactttc
     4141 aattattttt gaaactttaa caaaacatta attgtggaat aatatactga ttatcaaaaa
     4201 gatgatggtt tattttttcg gatttatatg gttaagtgac aggatattga gaggtttgtt
     4261 taacgcatag tgcattttcg ttaaacaaag tcttatattt acggaagcaa actaaagtac
     4321 aagctatgtc aattatagaa tttaataaaa agatagacaa catatctcag tatctgcatt
     4381 cctttgcatt gaatctgacc aagaattcgg aggatgcaaa ggacttgtat caggaaacaa
     4441 cctaccgagc cctcgtcaat aaagataaat ttgcaccgga taccaaccta aaggcatggt
     4501 tatttaccat tatgaaaaat atctttatca acaattaccg taagaaagtg aaggccaaca
     4561 ccatcatgga ctctactgcc aatttgtatt atctcaatgc gggcggttct gaacacaatc
     4621 aaggtgagac caatatcttt ctgaaagaat tgagtgatat gatagatcag ttggatgatt
     4681 cactcagggt gcctttcatt ttacattatg aaggatttaa atatcaggag attgcggatg
     4741 agttggagtt gccgctgggt accatcaaga gtcgtatatt ctttgcgcgc aaggagttga
     4801 agaaattgat caaggatcga tatagtaata tttttgatat cagattgaaa gataaaaaga
     4861 aagccgtata ggcataatgg gtttattaca aaagcagaga acaaggcatt gtaatttgtt
     4921 ctctgctttt ttttacaccc aagtgtactt tattttacca tactcatggt gaataacaat
     4981 gggttgagcc ggttgtgata agggctggcg acaatcaaat ccttcggcat gaagtaggta
     5041 ggaaaataat aatctaaatc ataagagggg aaggagtctt gttgataatg gaaataaatt
     5101 gatgcctaaa tatgctggaa atatttgggc aattagacca gaatgtccgg aaataaaggt
     5161 aagtaggtta ttacttatct acctaattcg cttagccccc taaacttcac ttctacttag
     5221 atacaattga ttttgtctgc ttgtcgcata acctggcaat atcaaataaa aaagccgatg
     5281 ctgaagacat cggctttttc gtaatatatt tggatattat ccacatttgc tgttaccgca
     5341 atctttacag gtcaggcatc cttcctggta atgcagattg gtggatgagc agttagagca
     5401 tttagccttg cgcgcttccg tgccatccgg tatatatcgc ttgagcgcac gggccacacc
     5461 attcttccag gtattgatgg attcgtctaa ctgaaggcta ttgatcagat ccaccacttt
     5521 ttcgatcggc atggagtaac gcaatgctcc ggatatcaac ttggcatagt tccagtactc
     5581 agggttgaat ttataagata atccctcaat tgtggtcttg taaccgcgac cattgataaa
     5641 ctggaagtcg tagcgagaag tgccgtcagc ctctttgttt ttgatgatca ggccttgatt
     5701 gacccacctg ggaatcagga ttccgtcctc atcgtcggcc aatccggtga atatttcaaa
     5761 ggggcgtcca tctatcaaa
//
LOCUS       DAOQQN010000201         5746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6133_length_5746_cov_4.993674, whole genome shotgun sequence.
ACCESSION   DAOQQN010000201 DAOQQN010000000
VERSION     DAOQQN010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6133_length_5746_cov_4.993674"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1984
                     /locus_tag="PLG24_08250"
     CDS             <1..1984
                     /locus_tag="PLG24_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HMZ24921.1"
                     /translation="YSSGNIKNFFFDDIEIDRYKADTVGPVVVSVVPTGQSSVRLIVN
                     EEIVEEDFRNPENYKMYDSFGRELTIQHVATSGLTADITITETFKPDVYFLTISGLHD
                     LAGNLMNKQTLKFNYFAYATPERYDVLINELMADPSPVVTLPESEYIELWNTSSKILL
                     ISQLKIVKGITFYDLPKRALAPDEYLIIVPSGDVDKWNGYNNVVGISPFPTLNNDGAM
                     LTLIDSAGNLIHHVSYDLSSYKDNSKKDGGWSLEHTVKEGICDGNDVWRASTDASGGT
                     PGRVNSNTVIASENVLLTGMELLDDRNLQLCFSKYLNFSDSVTQAAFSSVNTPSFQIF
                     FSQEVDNCCIIEFEESLMDAVWYELGISGYIVNCLGNYIEADTLIGFGWGFPKPGPND
                     IIINEILFDPNTGGSDFVEFYNRTSTTFDLRGLNIVNTQGNKISTINKTALLKPNDYL
                     AFCPDKNWLAANYTLRNPQNIIEIGIPSFNSDQGNVSLMDGISLIDSFRYNQKLHNVF
                     LTKVKGVSLERVSADGDSNQKSNWQSAASDAGGATPGYVNSQTRTLKNVDKILTKPED
                     HFSPNNDGFLDQFIVQYLLDKPGYILRWRVFDAAGRLIKSDQSGVLTGTDGFITWDGS
                     TDDLKKALPGIYVFKFQFTHPDGTTKSEHISCVLTY"
     gene            complement(2042..3400)
                     /locus_tag="PLG24_08255"
     CDS             complement(2042..3400)
                     /locus_tag="PLG24_08255"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HMZ24922.1"
                     /translation="MKLTPSVRQIMAIALPIMIGSAAQNVLQLSDSIFLYHLSETDFA
                     AIGFVGVFYLMVNAIGYGFSKGGQIIIANLAGRQDVVGIRRNFQALGLFEAMLALVMF
                     LFMFFAGPWFFSLFLQNQKVLDACNEFIKYRSFGVFFSYIGITLIALYTGIARPKFIM
                     IETIFLLIVNGLLNYALIFGKWGLPAMGIAGSALASTIAEALGAIVFLLYLYFDKKIK
                     YLRIFAKPRIEFKLIRDQFNLAAPIVAQNFVSLGAWFVFFAIIEDMGERALAISNLVR
                     VVYLILSIPTWGFSSATNTIVGNLMGQGNFLSILPSMKKTAILCFILTMMIALPIIIW
                     PDILLYPLLGSADMTLISESRVTLFVLLSIITFNCIISIYINGIAGTGATLFGLKIQV
                     VSAIVYLIAIYVVVDIMHSSLNVAWATELLYWGVQLIGVFLFFYKIDFEKLKYGKKSL
                     TA"
     gene            complement(3571..4695)
                     /locus_tag="PLG24_08260"
     CDS             complement(3571..4695)
                     /locus_tag="PLG24_08260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ24923.1"
                     /translation="MSWTIAFILILFAIYAFSIFLITIEWLKTRILNTNPELDGISFS
                     IIIPFRNEEKNIEQCIKSIVDQQYDPGKFEIICVDDQSSDRSAEIISTFAAKYKMIRL
                     IDSNGNGKKAALQTGIEVSRFNHIITTDADTDRGKHWLSAYSTMFRYRGIKVVIGTLL
                     MVGKGNSLLGAMQSLDYMGMMVFSMVASRKKWFYNGSGGNLAFTKDVYFDYLESAHDR
                     DIVSGDDVFLIEFVSSKFDKSVLFPRNQEIIAYTQPEPDFVSFLNQRKRWANKSFSYR
                     NNNILAFWAFLWIVNVSFIIALILAIINGGLFIKVFAAALFGKLIVDYFMLMPMGKFF
                     RRPINKFILADFYHILYVVLIGFLAFFNKSFVWKERKFSR"
     gene            complement(4724..5260)
                     /locus_tag="PLG24_08265"
     CDS             complement(4724..5260)
                     /locus_tag="PLG24_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HMZ24924.1"
                     /translation="MEIKKVQKNNSARKNISEDKTVEIPLNDKLFSEVKMIFSEFLEK
                     NQCRKTPERYAILEEIYRRGDHFDAETLYFHMKNHNYRVSRATVYNTLELLVNCDLVK
                     KHQFGKNLAQYEKSYGYKQHDHLICEKCGKVVEFCDPRIQNIKNMIGEMLNFNITHHS
                     LNLYGTCLGGCEAIQSEE"
     gene            complement(5290..>5746)
                     /locus_tag="PLG24_08270"
     CDS             complement(5290..>5746)
                     /locus_tag="PLG24_08270"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HMZ24925.1"
                     /translation="NKSFLLINGESADKFSYQFATCCHPLPGDDIFAFVTTANTLKIH
                     RASCPNSEHLLASYGYRVLKAEWANFVSSSFLEELVITGTDKGQGVILAITETINKFG
                     LNIKSFTIAGNEGVFEGLVKLEIRDLDQLNALIKALKNIPYISNVRRSN"
BASE COUNT         1812 a         1133 c         1060 g         1741 t
ORIGIN      
        1 atactcatcc ggtaatatta agaatttctt ttttgatgat attgaaattg acagatataa
       61 agccgacacc gttggcccgg tagttgtttc agtagttcca accggacagt cctcggtaag
      121 attaatagtt aatgaagaaa ttgttgaaga agattttcga aatcctgaaa attataaaat
      181 gtatgatagt ttcggaaggg aattaactat tcagcatgtt gccacttctg gtttgactgc
      241 ggatattaca attactgaaa cattcaagcc ggatgtctat tttctgacta tctcaggttt
      301 gcatgacctg gcaggaaatc ttatgaataa gcagacattg aaattcaatt attttgccta
      361 tgcaacacca gaaagatatg atgtcctgat caatgaactg atggcagatc catcgccggt
      421 tgttaccttg cctgaaagcg aatatattga attgtggaac acgagtagta agattctgtt
      481 gatcagtcaa cttaaaattg tcaaaggtat caccttctac gacctgccca agagggcttt
      541 agcacctgac gaatatctga tcatcgtccc ttccggagat gttgataagt ggaatggata
      601 taacaatgtt gtcggcattt cacccttccc tacactcaat aatgacggag cgatgctcac
      661 ccttatagat tctgccggta atttgatcca ccatgtaagt tatgacttat cgtcctacaa
      721 ggataattcg aaaaaagatg gaggctggag tcttgagcat accgttaagg aaggaatttg
      781 tgatggtaat gatgtctggc gggcttcaac ggatgcttca ggtggtacgc ccggcagagt
      841 caactccaac actgttatcg cttctgaaaa tgttttattg acaggaatgg aattacttga
      901 tgacagaaac cttcaattgt gcttttctaa atatttaaat ttttcagatt cagtgaccca
      961 agctgccttt tcttcggtca atacaccatc attccagata ttcttttctc aggaagtgga
     1021 taattgttgt atcattgaat ttgaagaatc tttgatggat gcggtatggt acgaattagg
     1081 gatttccgga tatatagtca attgtcttgg aaattacatt gaagcagata ctttgatagg
     1141 gtttggctgg ggttttccaa aacctggacc aaatgatatt attatcaatg aaattttgtt
     1201 tgatccaaat accggtggat ccgatttcgt ggagttctat aacaggactt ctactacctt
     1261 tgatctaaga gggttgaata ttgtaaatac ccagggaaat aaaatttcta caatcaacaa
     1321 aacagctctc cttaaaccca acgattatct tgcattttgt cctgataaaa attggctggc
     1381 tgcaaattat acactcagaa acccacaaaa tattattgaa attggcatcc cttcgtttaa
     1441 tagtgaccaa ggcaatgttt ctttaatgga tggtatttca cttatcgata gttttaggta
     1501 caaccaaaag ttgcataatg tattcctaac taaagttaag ggtgtttccc ttgaacgagt
     1561 ttctgcggat ggagattcaa accaaaaatc caattggcaa tccgctgctt ccgatgccgg
     1621 tggtgccact ccgggatatg tcaacagcca aacaaggacc ttgaaaaatg ttgataagat
     1681 attgacaaag cctgaagacc atttttcccc caataatgat ggttttctcg atcaatttat
     1741 tgttcaatac cttcttgata agcccggcta tatattacgt tggcgcgtat ttgatgcagc
     1801 aggccggctg attaaatcag atcagtccgg agtgttgacc gggacagatg gctttatcac
     1861 ctgggatgga agcactgatg atctgaagaa agcattgccg ggaatctacg tctttaagtt
     1921 ccagtttaca catccggatg gtacaaccaa aagcgaacat atcagttgtg tgctgaccta
     1981 ttaagattta attttatgct ttaaacaaag gactataagc gcctcttatt caatcaataa
     2041 attacgcggt caaactcttt ttaccatatt tgagcttttc aaaatctatt ttataaaaaa
     2101 agaggaaaac tccgatgagc tggacacccc aatataacaa ttccgttgcc caggcgacat
     2161 tcagtgaact atgcatgata tcgacaacca cataaattgc aatcaaatat acaattgcac
     2221 tgacgacttg tattttaaga ccaaataggg tggcgcctgt tccggcaatt ccattgatat
     2281 agatggaaat gatgcaattg aaagtaatga tactaagaag tacaaagagt gtaactctac
     2341 tttcactaat aagcgtcata tcagctgaac ccaacagagg atacaataat atatccggcc
     2401 aaataataat cggtaaggct atcatcatag ttaagataaa gcaaaggatt gctgttttct
     2461 tcatgcttgg cagaatgctc agaaaattgc cctgccccat taagtttccg actatggtat
     2521 tggtggcaga actaaagccc catgtaggta ttgataagat taaatagacc acccgaacta
     2581 aatttgaaat ggccagagcc ctttcgccca tatcttcaat aatggcaaag aaaacgaacc
     2641 aggcgcccag actaacaaaa ttttgtgcaa ctattggcgc agcaagatta aactggtctc
     2701 tgattaactt aaactcaatt cttggttttg cgaatattcg taagtatttg atttttttat
     2761 caaaatagag gtaaagcaga aagactatgg cacctagcgc ttctgctatt gtactcgcca
     2821 atgcacttcc agctattccc attgcaggta gcccccactt accaaatatc agggcgtaat
     2881 taagtaatcc gttgacaatc agcagaaaga tggtttctat catgataaac ttgggcctgg
     2941 ctattccggt atacaaagca attaaagtga taccgatata ggaaaaaaat acaccaaagg
     3001 atctgtattt aataaattcg ttgcatgcat caagtacctt ttgattttgc agaaaaagac
     3061 tgaaaaacca aggccctgca aagaacataa acaaaaacat aacaagggcc agcatcgctt
     3121 caaacagacc caacgcctgg aaatttctcc taatcccaac tacatcctgg cgaccggcaa
     3181 gattggcaat aattatttga ccgcctttac taaaaccata accgatggca ttgaccatga
     3241 ggtagaaaac accaacaaat cctatggccg caaagtcggt ttcacttaaa tggtacaaga
     3301 atatactgtc actcaactga aggacatttt gtgccgcact ccctatcatg atcggcaaag
     3361 cgatagccat aatctgtctg acggaagggg taagtttcat accatttgct gattgttcat
     3421 gaagcttatg ctaaaaatta atcttatatt cattttggac taactaagcc ttatccaaat
     3481 atagaaattt tcgtccgaat aaaacttacg gattgctcaa attctatttg gacctctaat
     3541 ctacattgaa gataatatta atttaaagaa tcaccttgaa aacttccttt ctttccaaac
     3601 aaatgattta ttgaaaaatg ccagaaatcc gataaggaca acatatagaa tatgatagaa
     3661 atcagctaaa ataaatttat taatcggacg tcggaagaat tttcccattg gcatcagcat
     3721 aaaatagtcg acaatcaact taccaaaaag ggcagctgca aataccttga taaaaaggcc
     3781 accattaatg atcgccaata tcaaggcaat aatgaaacta acattaacaa tccataaaaa
     3841 ggcccagaag gccaatatat tgttattcct gtagctgaat gacttattgg cccacctttt
     3901 ccgctggtta agaaaagata caaagtccgg ttccggttgt gtataggcta taatttcttg
     3961 attacgagga aataaaacag atttgtcaaa tttagacgaa acgaattcga tgagaaaaac
     4021 atcgtctccc gaaactatat cccgatcatg tgcagattcc aaataatcga aataaacatc
     4081 ctttgtaaaa gcaaggttac ctccacttcc attataaaac catttttttc tggaagctac
     4141 catcgagaat accatcatcc ccatatagtc cagggattgc atagctccca atagggaatt
     4201 acccttcccg accataagta atgtacctat aacgacctta atccccctat atctgaacat
     4261 agttgagtag gcggacaacc aatgtttgcc gcggtctgta tcagcatcag ttgttattat
     4321 atgattgaag cgtgacactt cgatgcccgt ttggagtgca gctttcttac cgttgccatt
     4381 actgtcaatc agcctgatca ttttgtattt ggctgcgaat gtgctgatta tttctgcaga
     4441 gcgatcacta gattgatcat caacacagat tatttcaaat ttacccggat catattgctg
     4501 gtctactata cttttgatgc actgttcaat atttttttct tcatttctga atggtatgat
     4561 aatcgaaaaa cttatcccgt ccaactcagg gttggtgttc agaatcctgg tcttcaacca
     4621 ttcgatggtg ataagaaaga tactgaaagc atatatagca aacagaatca gaataaatgc
     4681 tattgtccag gacatttagt attgtattta tctcccggga gaattattct tcagactgaa
     4741 ttgcttcaca tccacctaaa catgtgccat ataaattgag agaatggtgt gtaatattaa
     4801 aattgagcat ttcgcctatc atattcttga tattctggat gcgagggtca cagaactcaa
     4861 ctacttttcc acatttttca catatcaaat ggtcgtgttg tttgtaacca tatgattttt
     4921 catattgtgc aaggttcttg ccgaattggt gttttttgac cagatcacaa ttgacaagca
     4981 attcgagagt attgtatacc gtagctcttg aaaccctgta gttgtgattt ttcatatgga
     5041 aatacaatgt ctcggcatca aaatgatccc ctcttcgata tatctcttca agtattgcat
     5101 acctttcagg agtttttcga cattgatttt tctccagaaa ttccgagaat atcatcttaa
     5161 cttcagaaaa cagtttatca ttcaaaggaa tttcaactgt tttgtcctct gaaatatttt
     5221 tcctcgcact attgttcttt tgtacttttt taatttccat atttttaatt tacgagaaag
     5281 ctttgtatgt caattacttc ttctaacatt tgaaatataa ggaatgtttt tcaatgcctt
     5341 aattaaggca ttcaattgat ccaaatccct gatctcaagt ttcacaagac cttcgaacac
     5401 tccttcattg cctgctattg tgaaagattt gatattgaga ccaaatttgt tgattgtctc
     5461 tgtaatggca aggataacac cctgaccttt gtctgttcct gttattacca attcttccag
     5521 gaaagaagaa gaaacgaaat tagcccattc ggcctttaac acccgatatc cataggaggc
     5581 aagtagatgc tcactgtttg ggcatgatgc ccggtgaatc ttcagcgtat tggctgtggt
     5641 gacgaaagca aaaatgtcgt caccggggag ggggtgacag caggtggcaa actggtaact
     5701 aaatttatca gcgctttccc cattgatgag aagaaacgat ttattc
//
LOCUS       DAOQQN010000202         5718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6179_length_5718_cov_4.241215, whole genome shotgun sequence.
ACCESSION   DAOQQN010000202 DAOQQN010000000
VERSION     DAOQQN010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6179_length_5718_cov_4.241215"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="PLG24_08275"
     CDS             complement(<1..1364)
                     /locus_tag="PLG24_08275"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HMZ24926.1"
                     /translation="MPDINHPIEFVKGIGPRKAELLKKAFNVTTIGDLIMQFPFRYID
                     KTTVKPINQVTINDEWVLIKAKVLYTELAGKGRGRLIAHVSDGSGVMELVWFQGIKYI
                     ETLLEKGKEWLFYGKINVFNGKINLPHPELETPSVEGQPDFLLPIYSSTEGLTRAGFN
                     MKFRRELMSKWLNELTHNDFPEMIPEELIRTMQLPTRFGSIRHMHFPTSLKTAEQAKR
                     RLIFEEFFVNQTTLIMQKLYRKNYIHGYNFSIIGNYFNKFYNNHLPFDLTNAQKRVIK
                     EIRTDMGSGFQLNRLLQGDVGSGKTIVALMSALIAVDNGFQTCIMAPLEILAQQHYKN
                     ILRYTEPLGLKIALLTGTTKKKEREQILAELANGEISIIIGTHALIEPIVRFHKLGLV
                     IIDEQHRFGVEQRAALWSKATPLPPHVIVMTATPIPRTLAMSVFGDPDVSIIDELPPG
                     RKPI"
     gene            1619..2416
                     /locus_tag="PLG24_08280"
     CDS             1619..2416
                     /locus_tag="PLG24_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HMZ24927.1"
                     /translation="MNDVNLKQYFDRIGFAGTPDVGWDTLVHIQQNHIASIPFENLNP
                     LMRIPVELDGEAIFEKIVRKHRGGYCFEHNLLLQVVLERIGFTVRGLLGRAGQEPESV
                     GRTHMVLLVTLEGRDYLVDVGYGGLVPTGPLLLEPDIVQTTPNEDYRIISPDGEYRLE
                     IKLNGHWKRLYEFNLRKQIIQDFQVANWYTSTSPECIFTHTLVAARSEQGARYTLKDY
                     ELSIYQPDREIERRTLTSVDDVKAVLETIFYIDMSGLPKLEKYLRNA"
     gene            complement(2533..2847)
                     /locus_tag="PLG24_08285"
     CDS             complement(2533..2847)
                     /locus_tag="PLG24_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24928.1"
                     /translation="MGTQVMTYAFSGQTIDVTMTGSVLDRAYKLSVISFDQVKNKIVA
                     RTMRNKEVIYAVIFIKDIRHNRATIFKKEFSTLKEANEFGIPPANSTDSRGWNVFIKN
                     GN"
     gene            3214..4470
                     /gene="metK"
                     /locus_tag="PLG24_08290"
     CDS             3214..4470
                     /gene="metK"
                     /locus_tag="PLG24_08290"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HMZ24929.1"
                     /translation="MSYLFTSESVSEGHPDKVADQISDALIDHFLAYDETSKVACETL
                     VTTGQVILAGEVKSNAYLDVQEIAREVIHKIGYTKSEYMFEARSCGVLSAIHEQSPDI
                     NRGVERSDPENQGAGDQGMMFGYATNETANYMPLALDLAHKLLEVLAEIRRESKEISY
                     LRPDAKSQVTIEYDDDNRPVRIDTIVISTQHDDFDEDDSRMVEQIKSDIINILIPRVK
                     SQLKPATQALFDDKINYHINPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFS
                     GKDPSKVDRSAAYAMRHIAKNMVAAGLCNEVLVQVSYAIGVAEPCGVYVNTYGTSRVN
                     MTDGEIARKIQEIFDLRPYAIEQRLKLRNPIYSETASYGHMGRENRTVEKVFNKGKAN
                     EKKVTVELFTWEKLDYVDKVKEVFGI"
     gene            4552..5670
                     /locus_tag="PLG24_08295"
     CDS             4552..5670
                     /locus_tag="PLG24_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HMZ24930.1"
                     /translation="MIEQIKNTVRSLVVKTITFNLNTLDDDGRDIYLVGTFNDWNPQD
                     ERFKMERVKKGKYRLKVKLDENFPFPFEYKYTKGGWENEELTARGLKTKNRVALKMRR
                     SYTDRVARFLCNGSCSEARFRPIIEKFDKFPFPYLGVVRTVRVMLPYNYNADPHKHYN
                     VLYLQDGQNLWDPKAPFGNWDLEHKMTQMAMAGKTEVIIVAIDHGEADRIREYTPISG
                     TRIGRGDGQKYLSDVAKAVKPFIDNRYRTLRGREYTGLGGSSMGGLITLYGGLFYPDV
                     FGKLMIFSPSLWLIRDAQTEIPRFYTPFPTKVYLYAGGKESASMLPSMKRLKKALEEQ
                     HFFEKLVDVKLTIDPKGRHNENKWGREIVAAITWLYYK"
BASE COUNT         1578 a         1084 c         1437 g         1619 t
ORIGIN      
        1 ttaattggtt ttctgccggg tgggagctca tcgatgatgg agacatcggg atctccaaat
       61 acggacatgg ccagtgtccg tggaattggg gttgcagtca tcacaatgac gtggggcggg
      121 agaggggtgg ctttggacca aagggccgct ctttgttcca ccccgaaacg gtgttgctca
      181 tcgatgatga cgagccctag tttgtggaat cggacaattg gctcgatcag cgcatgagtg
      241 ccgatgatga tggaaatttc accgttagct aattctgcca atatctgttc tcgttccttc
      301 ttcttagtag ttcctgtaag cagggcaatc ttcaatccca ggggctcagt ataccggagt
      361 atattcttgt aatgctgttg tgccagtatc tccagggggg ccatgataca agtctggaat
      421 ccattgtcca cagctatcag cgctgacata agtgcgacga ttgtctttcc gctccccaca
      481 tcgccctgga gcaggcggtt aagttggaac ccgctcccca tgtcggtacg tatttccttg
      541 ataaccctct tttgggcatt ggtgagatca aatggcaggt gattattgta gaatttgttg
      601 aaataattac cgatgattga gaagttgtat ccatggatat agttctttct atacagcttc
      661 tgcatgatca aggtagtctg attgacgaag aattcttcga agatcagtct tcttttggct
      721 tgttcagcgg tttttaggct ggtcggaaaa tgcatatggc ggatacttcc gaatctggtc
      781 ggcaattgca ttgttcgaat gagctcttcg ggtatcattt ccggaaaatc attgtgggtg
      841 agttcattaa gccatttgga catcaattct ctgcggaact tcatgttaaa tccagccctg
      901 gtaagtcctt cggtggatga atagataggg agtagaaaat ccggctgacc ttcgaccgaa
      961 ggtgtttcca gctcgggatg tggcaggttg attttaccgt tgaagacatt gatctttccg
     1021 taaaaaagcc attccttgcc cttttcgagt aaggtctcga tgtacttgat tccctggaac
     1081 cagaccagtt ccatgactcc tgacccgtca ctgacatgtg cgatgaggcg gcctcgccct
     1141 tttcctgcta attctgtata gagtactttg gccttgatca acacccattc atcattgatt
     1201 gtaacctggt tgatgggctt gacagtagtc ttgtcaatgt aacgaaaagg gaactgcatg
     1261 atcaggtctc cgatggttgt cacattgaag gccttcttga gcagttcagc ctttctcggt
     1321 ccgatgccct tgacgaattc tataggatgg tttatatccg gcaaaatatt tcctgattga
     1381 cagaacaaag taaatgattt aaggcgatat atgcaataaa tcaatatatc acctgtcgta
     1441 ttaatgccaa ttgcttttag gcggttttgc tgcccgaaac gaacaaatat cggggtatgc
     1501 tgttagatta cacatcatca ttaccggcca tcttccgtca tgtagattag attggcaatg
     1561 gatgggtgat aatgccggag taatgatgtc catagagggc aaatactgat gtatggctat
     1621 gaatgacgta aatctaaagc aatatttcga ccgaataggc ttcgccggaa cacctgatgt
     1681 cggatgggac acattggtcc acatacaaca aaaccatatt gcctccatcc cgtttgagaa
     1741 tctcaatccg ttgatgcgta tccctgtaga gcttgatggt gaagccattt ttgaaaagat
     1801 tgtcaggaag catcgcggag gatattgttt tgagcataac ttgttgttgc aagttgttct
     1861 ggagcgtatc ggctttacgg taagggggtt gttgggcagg gcggggcagg agccggaaag
     1921 tgttggaagg acacacatgg tattgctggt cacactggaa ggcagggatt atttggtaga
     1981 tgtaggctat ggaggattgg ttcctaccgg tccgttattg ctcgagccgg atattgtaca
     2041 gaccacaccc aatgaagatt acaggatcat cagtccggat ggtgaatatc gtcttgaaat
     2101 taagctgaat ggccattgga agaggctata tgaattcaat ctcaggaaac agataatcca
     2161 ggattttcaa gttgccaatt ggtatacatc gaccagcccc gaatgtatct tcacccatac
     2221 attagttgcg gccaggtcgg aacagggggc cagatatacg ctgaaagatt atgagttgtc
     2281 catatatcaa cccgaccggg aaattgaaag aagaacgctc acatcggttg atgatgtaaa
     2341 ggcggttttg gagactattt tttacatcga catgtctggt ttgccgaagc ttgagaaata
     2401 tctgaggaat gcatgaataa ttgatacaca tttattaaaa ggagtcctta ggaaggtcgc
     2461 aattgcccgg catgaatttt gtgtctgaaa ataaatctca aaacagaatg tccctacgga
     2521 atgaatgcta cattaatttc catttttaat aaaaacattc cagcctctgc tgtctgttga
     2581 gttggcagga gggataccaa attcatttgc ttccttcaaa gtggaaaatt cttttttaaa
     2641 tattgtggct cgattatgcc tgatatcttt gatgaagatg actgcataga ttacttcttt
     2701 attcctcatt gttcttgcta cgattttgtt tttaacctga tcgaaagaaa taacggagag
     2761 tttataagct ctgtccagca cgcttcctgt cattgtaaca tcaatggtct ggcctgaaaa
     2821 ggcataagtc atcacttgag tccccatcag gggaattttg aacgaccagg aatattttcc
     2881 gctgatggga ataaatgctt cggccgttgg tgatatcggc agtcttgcag atcttatgaa
     2941 acagttgtct gagggccatt ttattgggat ggaatcgggc gatatgtcgg agtaattcac
     3001 ccatacaaga atgggtatta aaaacaagga aagtttcatc tatacatcat tgtttcttag
     3061 aaatgcaagc aatcaaactg attatggcgt caataattgt gtgaagacgc atattttcaa
     3121 tgaacgattg ccattcaatt cggttcaata aatatttgga aacatatatc aatttttgtt
     3181 atttttgccc ctcgaatcta aaaaattctt aaaatgtcgt atttatttac ttcagaatct
     3241 gtttctgaag gccacccgga taaagttgct gaccagattt ccgacgcact gattgatcac
     3301 tttctggctt atgatgagac gagtaaagtc gcatgtgaga cacttgtaac gaccgggcag
     3361 gttattcttg caggtgaagt aaaatccaat gcttatctgg atgtccagga aatagcaagg
     3421 gaagtaatcc ataagatcgg ctatacaaag agtgaatata tgtttgaggc gaggtcatgt
     3481 ggtgtacttt ctgcgatcca tgagcaatcg cctgatatca atcggggtgt agaacgttct
     3541 gatcccgaaa atcaaggtgc cggagaccag ggcatgatgt ttggttatgc aaccaatgaa
     3601 actgccaatt acatgccatt ggcgctggat cttgctcata agttgttgga ggtattggcc
     3661 gaaatcagga gggaaagtaa ggagattagc tacctgagac ccgatgcaaa gagtcaggtc
     3721 accattgaat acgatgatga taacagacct gtgagaattg atacaatcgt catttcgacg
     3781 cagcatgatg attttgatga agatgatagc cgaatggtgg agcaaatcaa gtctgatatc
     3841 atcaacattc tgataccgcg tgtgaagagt cagctgaaac ctgctacaca agctcttttt
     3901 gatgacaaaa taaattatca tattaatccg accgggaaat ttgttatcgg cggaccgcat
     3961 ggtgacacag gattgaccgg aaggaagatc atagtggata cttatggcgg taagggagct
     4021 cacggcgggg gagctttctc cggaaaagac ccttcgaagg tagatcgttc ggcagcctat
     4081 gcaatgaggc atattgccaa gaatatggtt gcagcaggtc tttgcaatga agtattggtt
     4141 caggtatctt acgcgatagg tgttgccgag ccttgtggtg tgtatgtcaa tacctacggt
     4201 acttccaggg tcaacatgac cgatggtgaa attgccagga agatacagga aatattcgac
     4261 ctaagaccgt atgcgatcga gcagcgttta aaattgcgta atccgatata cagcgagacg
     4321 gcatcctatg gccacatggg acgcgaaaac aggacggttg agaaagtatt caataaaggc
     4381 aaggctaatg agaagaaggt taccgttgaa ttatttacct gggaaaaact cgattacgtg
     4441 gataaggtaa aagaagtatt cggtatttaa ttgcctgata atcaataaag aattgaatgc
     4501 atctgtctgg taggtaatgg ccgggtagat gcattttttt acagatttct tttgatagag
     4561 caaataaaaa atacggtccg gagtcttgtt gtcaagacca tcacgttcaa cctcaatacg
     4621 ttggatgatg atggtcgtga tatttatctt gtgggtacct tcaatgactg gaatcctcaa
     4681 gatgaacgct ttaagatgga gcgtgtcaag aagggtaagt accggcttaa ggttaagttg
     4741 gacgaaaatt ttcccttccc attcgaatat aaatatacta aaggaggctg ggagaatgag
     4801 gaattgactg cgcgaggatt gaagaccaag aatagggtgg ctcttaaaat gcgaaggagt
     4861 tatacggacc gagtagcacg atttctatgc aacgggtctt gttcagaagc cagatttagg
     4921 cccatcatag agaagttcga caagttcccc tttccatacc tcggtgttgt gaggactgtg
     4981 cgtgtcatgt tgccctacaa ctataatgct gatcctcaca agcattacaa tgtgctttac
     5041 cttcaggatg gtcagaatct ttgggatccg aaagcccctt ttggcaactg ggatctggaa
     5101 cataagatga cacagatggc tatggccgga aagacagagg tgatcattgt ggccatcgac
     5161 catggtgaag ctgaccggat aagagaatac actccaatca gcggaactcg tattgggcgt
     5221 ggtgatggcc agaagtacct gagcgatgtt gctaaggctg tcaagccttt tattgacaac
     5281 cggtacagga ctttgcgtgg tcgtgagtat accggactag gcggcagttc tatgggaggg
     5341 ttgattacct tgtatggagg tttattttat ccggacgtat ttggaaagct gatgattttt
     5401 agcccttcgt tgtggttgat ccgggatgcc cagacggaga taccacgttt ttacactcca
     5461 tttccgacca aggtatatct atatgcaggg gggaaggaga gtgcgtcaat gttgcctagt
     5521 atgaaacggt tgaagaaggc attggaagaa cagcattttt ttgagaagct tgtcgatgtc
     5581 aagttgacca ttgacccaaa aggccggcat aatgagaata aatggggtag ggagatcgta
     5641 gctgctatca cgtggttgta ttataagtaa tcgacaagtt atagggagat ttaggaggga
     5701 gtcctgaaga agggaaat
//
LOCUS       DAOQQN010000203         5681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6255_length_5681_cov_5.617846, whole genome shotgun sequence.
ACCESSION   DAOQQN010000203 DAOQQN010000000
VERSION     DAOQQN010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6255_length_5681_cov_5.617846"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1727
                     /locus_tag="PLG24_08300"
     CDS             <1..1727
                     /locus_tag="PLG24_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HMZ24931.1"
                     /translation="KPQIPHQSSATGMQAIPATGAALGMWYKEQQGMDQGDIPPLVVC
                     SIGDAAVTEGEVSEAFQMAALKSLPIVFLVQDNKWDISAFVDEVRSWNAAEYVRGIRG
                     LDSVSIDGSDFNESYDTLRHVFSIVRKERRPFVVHARVPLLNHHTSGVRKEWYRFDLE
                     EAVLDDPYPKLTTFLLEEGLISQRELEVLESRIRISIKQQYEQASNSEDPKPEDLYTH
                     IFAPTPITEEQGNRHGKDNTVKNMVDSGIFAIRELMLEDERCVLYGQDVGARLGGVFR
                     EAATLGEQFGSHRVFNTPIQEAFIIGSTVGMSAVGLKPIVEVQFADYIWPGLNQLFSE
                     VSRSNYLSNGKWPVSMVLRVPVGAYGSGGPYHSSSVESIVANIRGVKVVYPTDAADLK
                     GLMKAAYYDPNPVVIFEHKGLYWAKLPGTENAKCIEADSDYVVPIGKANILVESKDIN
                     DQPTCTVIAYGMGVHWAFNAEKAFPGRVEIIDLRSLVPLDTETIYQSVNKTGRCLVIT
                     EEAVDGSYAQSIAARVQENCFTALDAPVRTIGSENVPAVPLNETLEKTMLLNIDKVTK
                     AIESLLVY"
     gene            1741..2316
                     /locus_tag="PLG24_08305"
     CDS             1741..2316
                     /locus_tag="PLG24_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24932.1"
                     /translation="MLRQLLKLTFVFALLLTGLNLSAQKNVVFELDGGYSMIGDNSQT
                     GFGLNADLGYTFWGRLTPTVGYQFHTFANQRNHYDTNANIDLAYLAVKVTALKFKWFS
                     LDLGPLAFRRMWKQEYKVHDGYTVYKDGLTMDGNNIYSYYKPQWGYGGVAGVKFDLFK
                     KSGVKLDVTYQIDTDRKDSMMFYSGGVFFRF"
     gene            2809..4029
                     /locus_tag="PLG24_08310"
     CDS             2809..4029
                     /locus_tag="PLG24_08310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ24933.1"
                     /translation="MKKLFLILFVFVLNCPVFSQDPVFTLVKVGFDNFNFFYDIKASP
                     EGTLWVCSNEGNVFRKLSSSTTWEHISDYPGTGAEAKALFVAADDDVWIISYGNGIFH
                     FNGTKWEEVSLTNVPETNKWNCVSGDINGNVWFGSNDKGLYKYSAGQWTNYSMSNSGI
                     LSDQINDLLATPSGKLFVGGYGTLQTFESNNWVDLGFTSNIGFGERIYSFAIDNDGLV
                     YIATTKGLFKFKDDKLEIYGDTKNRDVYYAVFDRNNVLWFFGEEQAFDNYLYRCDNGT
                     CKKLDDKNLPHQCSGLAVGSDNLLYLIGSLGPNILVIEDKTTNSDKELLRQPMVEISP
                     NPASDFLSIKSPQTLKEISLFNSAGQSLFAAKNINQTHFMLNLQGRGKINPGVYVLLI
                     KTEIGTCRKKIMIQ"
     gene            complement(4344..5198)
                     /locus_tag="PLG24_08315"
     CDS             complement(4344..5198)
                     /locus_tag="PLG24_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24934.1"
                     /translation="MKLRLLLIFLTVSICSNAQTFQEGTNLQAEYSKISQPYFFEAPC
                     YTSWIEGANLLLIGDRPDQNDCDFVFLALHDTTLIGVYKTQEPHYFIFDTEGNSILSK
                     PTAYFQLPLWTVMKSIHIDSSDMKVLKVLDKFYEMTLQDNYLDLNAATLKEFNKYQTN
                     TSLANRHIVLLFYNYLSFIGQAAEGGEQPPPDICLQLIKSLSDACFSLYKKIPVIVRV
                     YSVEALINAGMIDEAREYIKNSLCFYPGSIPLMVYNYKLESDNDMKKRQLVLLKKKYG
                     SHWMVKDL"
BASE COUNT         1708 a         1058 c         1250 g         1665 t
ORIGIN      
        1 acaaaccaca gattcctcat cagtcatcag ctaccggtat gcaggccata ccggctacag
       61 gagctgcgct gggcatgtgg tataaggagc agcaagggat ggatcagggt gatattccgc
      121 cattggtcgt gtgttccatc ggggatgcag ccgtgacgga gggtgaagta agtgaagctt
      181 tccagatggc ggccttgaag agcttgccga ttgtattcct ggtccaggat aacaaatggg
      241 acatatcggc cttcgtggat gaagtgcgca gctggaatgc agcagaatat gtaagaggaa
      301 tccggggact ggattctgtt tcgatcgatg gttctgattt caacgagtcg tacgatacac
      361 tcagacatgt attttctatc gtcagaaagg aacggcgacc ttttgtcgta catgccaggg
      421 taccattgct taatcaccat acttcgggag tgcgtaagga gtggtatcgg tttgatcttg
      481 aagaagctgt acttgacgac ccttatccca aactgactac tttcctgttg gaggagggat
      541 tgattagtca gagggagttg gaagtactgg agtcccgtat caggatttcg atcaaacaac
      601 aatacgagca ggcaagcaat agcgaagacc caaagcctga agacctgtat acacatatct
      661 tcgctcctac acccattacg gaggagcaag gcaacaggca cggcaaggat aatactgtca
      721 agaacatggt ggatagcgga atattcgcca tccgagagct catgttggaa gacgaacgtt
      781 gtgtccttta tggccaggat gtaggtgcca gattgggagg tgtatttcgt gaggcagcta
      841 cacttgggga acaattcgga agccatcgtg tattcaatac tcccatccag gaagcattca
      901 tcatcggaag tactgtgggc atgtcggcag taggccttaa gcccattgta gaagtgcagt
      961 ttgccgatta tatatggccc ggacttaacc agttgttttc agaagtcagt cgatccaact
     1021 acctgtccaa cggaaaatgg cctgtcagta tggtgctcag ggtgcctgta ggggcatacg
     1081 gcagcggtgg accttaccac tccagcagtg tggagtctat agtagctaac atacgagggg
     1141 tgaaggtcgt gtatccaacc gatgcagcag acctgaaagg attgatgaaa gcggcctatt
     1201 acgatcccaa tcctgttgtg atattcgaac acaaaggctt atactgggct aaattgccgg
     1261 gtacggaaaa tgctaaatgt attgaggcag acagtgacta tgtggtccct atcggcaagg
     1321 caaatatttt agttgaatcc aaagatatca acgatcagcc aacttgtacg gtcattgcct
     1381 atggcatggg cgtgcactgg gcgtttaatg cagaaaaagc cttcccggga agggtggaga
     1441 tcatcgacct gagatcgttg gttccattgg atacggaaac aatctatcaa tcggtaaaca
     1501 aaacaggccg ttgtctcgtc ataacagaag aagctgtcga tggctcgtat gctcaaagta
     1561 tcgctgccag ggtccaggaa aattgtttca ctgcccttga cgcgcctgta cgtacaattg
     1621 gctcggaaaa tgttccggct gtgccattga atgagacgtt ggaaaaaacc atgttgttga
     1681 atatcgacaa agtcaccaaa gctattgaat cattgttagt ttattaaaat tctaaaataa
     1741 atgttacgtc aattactaaa actcactttt gtatttgccc tgttgctaac aggtttgaat
     1801 ctttccgctc aaaaaaatgt cgtctttgaa ttggatggcg gatactccat gattggagac
     1861 aacagccaga ccggcttcgg gctaaatgct gaccttggat atactttttg gggtcgtttg
     1921 acacctactg tcggttacca gtttcacacc tttgccaatc agcgtaatca ttatgatacc
     1981 aatgccaaca tcgatctggc ataccttgca gtcaaggtta ccgcattgaa attcaagtgg
     2041 ttttcccttg atctaggccc tttggcattc cgtcgcatgt ggaagcagga gtataaagta
     2101 catgatggat acacagtcta taaggacggt ctgaccatgg atggcaacaa tatttattcc
     2161 tactacaagc ctcaatgggg ctatggtggt gttgccggtg taaaattcga tctattcaag
     2221 aaatccggtg tcaagctgga tgttacttat cagatcgata ccgaccgtaa ggatagtatg
     2281 atgttttact caggcggtgt attctttaga ttttaaggag tcagaaaccc tctgtctatc
     2341 gccaatcgac ggatgatatt catccgtaaa gtcggttgtc aggctgtaat cttttaagat
     2401 ggcagacaag tattggcaga atataataca agtcattggc gtccaacagt acaaagaaag
     2461 gaatattctc ctgacttcgg catttagtaa attatgaaaa ttataaatcg ctaataatcc
     2521 atagcttggc aaccgatcgg gcaacaacac gtctttttgg ggtgtacatc ccgggatcaa
     2581 cttgtgtatc cggttgatag gatgaggtgt ttccaccggg tatgcatggc tagttgataa
     2641 catccgttct aaaataacgg gcaaatcatc ctctgtctta tcttacaatc cccggtaagc
     2701 agattttgca gtttaatgaa aggaataatg catgatgaat tataaaattc aaattattat
     2761 tgatatattt gaatctctca ttatacaata actaaaaatc aattaattat gaaaaagcta
     2821 tttcttattc tctttgtttt cgttttaaat tgtcccgttt tctcacagga tcccgtgttt
     2881 actcttgtta aagtcggctt tgacaatttt aatttctttt atgacataaa agcgagtccc
     2941 gaaggaacat tatgggtgtg ttctaatgaa gggaatgtat tcaggaaatt aagttcctct
     3001 acgacctggg agcatattag tgactatccg ggaactggtg ccgaggcaaa agcattattt
     3061 gttgctgcag atgatgatgt ttggattatt tcatatggga atggtatttt tcattttaat
     3121 ggcacaaaat gggaagaagt ctctcttacc aatgttcctg aaaccaacaa atggaattgt
     3181 gtttcaggag atataaatgg aaacgtatgg ttcgggtcca atgataaggg cctttataaa
     3241 tatagtgccg ggcagtggac aaattacagt atgtccaatt cgggtatttt gtcagatcaa
     3301 ataaacgatc tgttagcgac accttcgggt aagttgtttg tgggaggtta cggaacttta
     3361 cagacttttg agtcaaataa ttgggtagat ttaggcttta cttccaatat tggttttggt
     3421 gaacgcatct atagttttgc catcgataat gatggcctgg tctatattgc cacaactaag
     3481 ggactattta agtttaaaga tgacaaatta gagatttacg gagataccaa aaatagagat
     3541 gtttattacg cggtttttga ccgtaataat gttttgtggt tttttggtga agaacaggct
     3601 tttgataact acctgtatcg ctgtgacaat ggaacttgca agaaactaga cgataaaaat
     3661 ttacctcatc aatgttcggg tctggctgtt ggaagtgata atttattata tttaatcggg
     3721 tctttaggac ccaatatctt agttattgaa gacaagacaa cgaattcgga taaagaatta
     3781 ttgagacaac caatggtaga gatttctcct aatcccgctt cagatttttt atcaataaaa
     3841 tccccgcaaa cgttgaaaga aatttcacta tttaatagtg caggtcaaag tttatttgca
     3901 gctaaaaaca ttaaccaaac ccattttatg ctgaacttac agggacgtgg taaaataaat
     3961 cccggagtgt atgtcttatt gattaagaca gaaattggaa cttgcagaaa aaagataatg
     4021 attcaataaa tagttaacaa tacttcggaa agttaaaata aaatatatgt taaatattcc
     4081 ggacaaattg ggccatcaat tccggtcaca agtgagccta tatcacgaac tatttggaaa
     4141 ttcaataaaa gaatgagtaa caacatgaag ccttcatagc ccactaagaa tttatttttt
     4201 catattccct ccgcggaggt attctccttt gagatcaatc ctgattgccg taaatgaccg
     4261 aacaacaacc tcttacccaa tgtcatacta taatgacata atcacagcaa aaaaactttt
     4321 accatttcat aagcataaga attctataag tcctttacca tccaatgcga accatacttc
     4381 tttttcaaaa ggactaactg tcttttcttc atatcattat ccgactccag cttataattg
     4441 taaaccatta aaggaataga cccgggataa aaacacaaag aatttttaat atattctctt
     4501 gcttcgtcta tcatacctgc atttatcagg gcttcaacac tatatacgcg tacaattacc
     4561 ggaatttttt tgtacagtga aaaacacgca tcagacaagg attttataag ttgaagacaa
     4621 atatccggtg gaggttgctc ccctccttca gcagcctgtc caatgaagga cagataattg
     4681 taaaagagta aaacgatgtg cctgttggcc aaagaggtat tcgtttgata cttattaaat
     4741 tcttttaaag tggcagcgtt gagatcaaga taattgtctt gtaaggtcat ttcgtagaat
     4801 ttatccagga ctttcaaaac cttcatatca gaagaatcta tatgtatact cttcattaca
     4861 gtccataaag gaagctgaaa gtatgcagta ggtttactga ggatggagtt tccttctgta
     4921 tcaaaaataa aataatgtgg ttcttgtgtc ttgtacactc cgattaaggt cgtatcatgc
     4981 agggcaagaa atacaaagtc acagtcgttt tggtcaggtc ggtcaccaat caaaagtaaa
     5041 ttggcaccct caatccaact tgtataacag ggtgcttcaa agaaatatgg ctgactaatt
     5101 ttactgtact ccgcctgaag atttgtgcct tcctgaaaag tctgtgcatt agaacagatt
     5161 gaaacagtaa ggaagattaa aagtaatcgc agcttcatct ctgcatctat taagtattat
     5221 cccggtaatt aaagaacaaa tataaccatt tgatacaatt gattttggga tgaagatgta
     5281 accgaccagg caactacacc tcattttttc ggatatatcc ggggagcagt tcatgtatcc
     5341 ggtttaaagg gtgcggcgtt tccaccggct attcatggag gttgatccta atatctttta
     5401 acgatccgcc acctcttcgg agttcttgat agtgcatata ttactgatta ggtatgaccg
     5461 tagatggctt aaaaagatgt gccttggcgg ttgattacgg aaatttgggg gaaatgatta
     5521 tatttatacg gaaagaggct gtcccaaaag gatggccttt tttatgcaat taatttctga
     5581 gcggaggggt tgaaatggaa atttttgttt ttgaaaatga tatttttcaa attttgaaaa
     5641 taatagccta aaaatgagcc taaattaatt ggagaggaag g
//
LOCUS       DAOQQN010000204         5670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6276_length_5670_cov_4.585574, whole genome shotgun sequence.
ACCESSION   DAOQQN010000204 DAOQQN010000000
VERSION     DAOQQN010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6276_length_5670_cov_4.585574"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PLG24_08320"
     CDS             <1..1009
                     /locus_tag="PLG24_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24935.1"
                     /translation="NRYLTSALQASGLFEVTNEEITRVNYSRLGDYSLIIINEVINVS
                     SGLLDELSKALSGGANVAIFPGIRSQSAISQLSGKFSLGTAGLMKNVSQQVSTIDTKD
                     YIFQNVFEGRTSQLKLPTSQSSMDINVRSTKGAIPLLTYRSGKAFLVRYPRNGGQIFL
                     CAAPLDPEYSDFVKNGEILVPFLFKAGFSNLRKQLAFIIGRNESFEINQFLEGRDAVL
                     KFTGPQTFIPEQLRKGRKTLINTGRESILPGVYSLEGAGLYAFNYDRQESRMDFLSAD
                     QLKTYENQFIKVWDHKDQVNLASIIREEDTGLSLWKYLLIGALLFLLAETALLRSGQK
                     A"
     gene            1083..2309
                     /locus_tag="PLG24_08325"
     CDS             1083..2309
                     /locus_tag="PLG24_08325"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HMZ24936.1"
                     /translation="MSIETIFLLFSLFVWPGCNADKPILDNKVTEHVLGEAEGLPLYT
                     GADQVDKYMPILKGARVGLLINQTSMVGDKSLVDVMVENGVRVQAIFAPEHGFRGEAD
                     AGAEIPDGKDKLTGIPIISIYGQKKAPDAGDLKDIDVVVFDVQDVGARFYTYLSTLKY
                     LMEACARNNVSLLVLDRPNPNGHYVDGPILEPAFTSFVGVIPIPIVHGMTLGELAGMM
                     NGEGMLAEGIKCRLKVVTCQNYNHHVPYKLPVKPSPNLPNQLSVLLYPSLCLFEGTNF
                     SVGRGTDKQFQIYGSPDAGEGDFYFTPQPKPGAMKPFLEGKKCRGFDLSGMKEVEVMK
                     EGRLNLSYILKAYASCKEKKTFFLKNNFIDKLAGTDKFRKQVVSGLSEDQIRESWQPG
                     LSTFKEMRKKYLLYPD"
     gene            2361..3506
                     /locus_tag="PLG24_08330"
     CDS             2361..3506
                     /locus_tag="PLG24_08330"
                     /inference="COORDINATES: protein motif:HMM:NF005293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HMZ24937.1"
                     /translation="MTNFNEVYITRISTFLPNQPIGNDEIEQILGFAGGRPSRSRAII
                     LRSNGIKRRYYALDSNGNFRYSNAEMVARAIEGLFDEDIPQSKMEVLACGTSSPDMLL
                     PSLASMVHGELNAPALDIISAAGACCTGIQALKYAYYAIVTGEAETAVATGSERVSQY
                     LQGKKFDLEMEDMKALEDNPILAFEKDFLRWMLSDGAGAFLLRNKPQPGSINFKIEWI
                     EIGSYADQLDTCMYLGADRNGAARLRSFGEMEVDDWGKRSVFSYKQDVRALGDNIVPF
                     GVKFLKSVLSKRKIDTTEIDYFLPHLSSMFFWKKIVEEFDKEGVDIPESKWFINLPEV
                     GNIGAASAYAMLQGLTERKDLVKGNKVMLFVPESARFSYAVVYLTVC"
     gene            complement(3992..4810)
                     /locus_tag="PLG24_08335"
     CDS             complement(3992..4810)
                     /locus_tag="PLG24_08335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ24938.1"
                     /translation="MTQLKNFIKFYLKATTLYNVHSSMVYQLLHFLFNNKIPESRASA
                     YASVRKNLLNNNNTIENHDLGAGSHKNIRGNTISISHIAKTSMSGQRKMQWLYNMSAY
                     FRPSTAIELGTSLGLSGSHIAANAERLITIEGNSAIAAVAEQTFRMLNNSNIQCLIGN
                     FDRVLPELLTIEQPDFIYLDGNHTYQATTEYFHLILEKTAGRRPVLVFDDIYWSSGMV
                     KAWNEIKAHPEVRLTIDLYHFGLVLFNEDIIAPRHYKVVPYLWKPWHIGFKPVH"
     gene            4892..5401
                     /locus_tag="PLG24_08340"
     CDS             4892..5401
                     /locus_tag="PLG24_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24939.1"
                     /translation="MRLLFAMLFFFLWLAAACGQKYRTAAGIRIGTEFGITVQQLILE
                     NSTLEGIIQKGFFNDQTTISVLFEQHQKLVFKGLNFYFGGGPHVGLYDANDRNGRKNS
                     FGLSAIGGVEMRFGKVLASFDYKPAINFFGGEHIFDSQSAISLRYIIVPAKKKEAKWK
                     FWEKKKKKK"
     gene            complement(5478..>5670)
                     /locus_tag="PLG24_08345"
     CDS             complement(5478..>5670)
                     /locus_tag="PLG24_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24940.1"
                     /translation="YVEAHEKVDECWLIDMKGTVLKRQKINETLTEINISQLSPGNYL
                     ILPIKDNKAYRGLMIQIVR"
BASE COUNT         1601 a         1051 c         1318 g         1700 t
ORIGIN      
        1 gaatcgttat cttacatcag cactgcaagc ttccggatta tttgaggtta ccaatgaaga
       61 gatcacacgt gtcaattata gcaggttggg agattattct ctcatcatca ttaatgaagt
      121 aataaatgta agttccggac tgctggacga actttcaaaa gcattgtccg gtggtgctaa
      181 tgtggctatt tttccgggca tcaggtcaca atctgccata agtcaactat ccggaaagtt
      241 ttctcttggg actgcaggat tgatgaaaaa tgtttcacag caagtaagta ctattgacac
      301 gaaagattat atcttccaga atgtttttga aggcaggacc agccaattga agcttccgac
      361 cagccaaagc agtatggata tcaatgtacg gtccaccaag ggagcgattc cgttacttac
      421 ctatcggagt ggaaaagctt ttttagtaag atatccgaga aatggaggac agatttttct
      481 ttgtgcagca cctttggacc ctgaatatag tgactttgtg aagaatggag agatcctggt
      541 tccctttctg tttaaagccg gattcagcaa tctcaggaag caattggctt tcatcatagg
      601 gcgaaatgag agtttcgaaa tcaaccaatt tttagaaggc agggatgcag tattgaaatt
      661 tacgggtccc cagacattta ttcccgaaca attgcgtaaa ggtcgtaaga cactgatcaa
      721 taccggacgg gaaagtattt tacccggtgt ttattctctt gaaggtgccg gcctgtatgc
      781 ttttaattat gacagacagg aaagtcggat ggactttctg agtgccgatc agcttaagac
      841 gtatgagaat caatttatca aggtatggga tcataaagac caggtcaatc ttgcttcaat
      901 catccgggaa gaggatacag gattgtcttt gtggaaatac ttgctgatag gagccttatt
      961 gttccttctt gccgaaactg ctctgcttcg aagtgggcaa aaggcctgat tttacatacg
     1021 gaattacacc tttggcaatg ggtgtatgta tattatctta aatttgagga aaaatcattc
     1081 aaatgtcaat tgaaaccatt ttcttattgt ttagcttatt tgtgtggcct ggttgtaatg
     1141 ctgataaacc tatattggat aataaagtca cggaacatgt tttgggtgaa gcagaagggt
     1201 tacccctcta taccggtgcg gatcaggtag ataagtatat gccaattctc aaaggagcca
     1261 gagtaggcct gcttatcaac cagacttcga tggtagggga caaatcattg gtcgatgtga
     1321 tggttgaaaa tggtgtccgg gtacaagcaa tcttcgcgcc tgaacatgga tttcgcggag
     1381 aagcggatgc gggtgctgaa atcccggatg gcaaagacaa gctgaccggg attccgatta
     1441 tctcaatata tggtcaaaag aaggccccgg atgccggtga tttgaaggat atcgatgtag
     1501 tggtttttga cgtccaggat gtgggggcaa ggttctatac ctacctgtct accttgaaat
     1561 atctgatgga agcttgtgcc agaaacaatg tgtccttgtt ggtactggac agacctaacc
     1621 ccaatggcca ttatgtcgat ggacctatcc tggaacccgc ttttacatct tttgtaggtg
     1681 tgattccgat tccaatagtt catggtatga cactcggcga gttggctgga atgatgaatg
     1741 gagagggcat gcttgccgaa ggaatcaagt gcaggctcaa ggtggtaact tgccaaaact
     1801 acaatcatca tgtgccatat aagcttcctg taaaaccatc cccaaatctg cccaatcagt
     1861 tgtcggtttt gctttatcca tccctttgcc ttttcgaagg aactaatttc agtgtcggtc
     1921 gtggtacaga caagcaattt cagatctatg gcagccccga tgcaggggaa ggcgatttct
     1981 acttcacacc acagcccaaa cccggagcaa tgaaaccttt tctggaaggg aagaagtgca
     2041 ggggttttga tcttagcggt atgaaggaag ttgaagttat gaaggagggg agacttaacc
     2101 tttcctacat cctcaaagca tacgcatcct gtaaggagaa aaaaactttt tttctgaaaa
     2161 ataatttcat agacaaattg gcaggaacgg ataaattccg aaaacaagtt gtctcaggcc
     2221 tttctgaaga tcagattcgg gaaagctggc aaccaggtct ttcaacattt aaagaaatgc
     2281 gaaaaaagta tttattatac cctgattgaa aatttgctaa ttttgcggtc tcttatcaga
     2341 gtgtattgta atttaaaata atgacgaatt ttaacgaagt atatattaca cgcattagta
     2401 cttttttgcc caaccagcca attggcaatg atgaaataga gcagatactt gggtttgcag
     2461 gtggtcgccc ttctcgttca cgggctatca tacttcggag caatggtatc aaaagaaggt
     2521 attatgccct cgattccaat ggaaacttca ggtattccaa tgccgagatg gtagcgagag
     2581 ctattgaagg cttgtttgat gaagatatcc ctcaaagtaa aatggaagtt ctcgcttgtg
     2641 gcacatctag cccggatatg ttgctacctt ctcttgcctc tatggtgcat ggcgaattga
     2701 atgcgcccgc attggatatc atcagtgccg ccggggcttg ttgtacgggt attcaggcat
     2761 tgaagtatgc ctattatgca attgtgacag gtgaggctga aacagcagtt gcgacaggta
     2821 gtgagcgtgt ctcccagtat cttcagggta aaaagtttga cctggagatg gaggatatga
     2881 aggcacttga agataatcct atacttgcct tcgaaaagga ttttctgaga tggatgctgt
     2941 cagatggagc cggtgcattc ctcttgcgca ataaaccgca accgggtagt atcaatttca
     3001 agattgagtg gatcgagatt ggatcttatg cggaccagtt ggatacatgt atgtacctgg
     3061 gtgccgatag aaacggggca gccaggttaa gatcctttgg agaaatggaa gtggatgatt
     3121 ggggaaaacg aagtgttttt tcatacaaac aggatgtaag ggctttgggt gataatattg
     3181 ttccttttgg tgtaaagttt ctcaaaagcg tcctgtccaa gcgtaaaatt gatacaacgg
     3241 agattgatta ttttctgcct catctatctt cgatgttttt ctggaagaaa atagtcgaag
     3301 agtttgataa ggaaggagtg gacatacctg aaagcaaatg gtttattaac cttccggagg
     3361 taggtaatat tggcgcggct tccgcctacg ccatgctaca gggactcact gaaagaaaag
     3421 atttggttaa aggcaacaag gttatgctgt ttgttccgga gagtgcccgc tttagctacg
     3481 ctgtagtcta tctgacggtc tgttgattgt attaatttat gttccttaca atagcaaatg
     3541 caaccttgtt gcataataag gagttgacaa aatttcgaaa gcagttgttg ggttgtggag
     3601 gttgttacca ttacgaatta ggtagatgaa taataccgat ccatccacct taggcaagtc
     3661 attagtcggt ctctttcaac aaatcagtcg gtatagagga gtaaaataaa ctcggaggtt
     3721 gctgcatgca gtctatgact gaatttgtag gtattaacca actacctaat tggatttttc
     3781 cgggtcattt tccgaattag gtagatgaat aataccgatc catctatctt aggcaagtca
     3841 ttggccggtc tctttcaaca aatcagtcgg aatagaggag taaaattaaa tcggaatttc
     3901 cagtatgcag tctatggctg aatttatagg tattaaccta ctacctaatt ggatttttcc
     3961 gtacatcttt ccaattcact cgtcttttcc gttaatggac cggtttgaag ccaatatgcc
     4021 atggtttcca aaggtagggc actaccttat aatgccttgg tgctatgata tcctcattga
     4081 acaagacaag gccgaaatgg tataggtcaa tcgtaagccg aacttccgga tgggctttga
     4141 tttcattcca ggccttaacc atgccggatg accaatatat atcatcaaat accagtacag
     4201 gccttcgacc tgctgttttt tcgagtatta agtggaagta ttcagttgtg gcctgatagg
     4261 tatggtttcc atcaagatag atgaagtccg gttgctcgat ggtcaataat tccggtaaaa
     4321 ctctgtcaaa gttgccaatc aggcattgta tgttgctgtt atttaacatc ctgaaagttt
     4381 gctcagcgac cgcagcgata gcagaatttc cttcgatggt gatgagcctt tcagcatttg
     4441 cggcaatatg tgatccggaa agccctagtg atgtgcccaa ttctattgct gttgatggcc
     4501 tgaaatatgc agacatgtta tacagccatt gcattttacg ctgtccggac atcgaggttt
     4561 tggcaatatg gctgatgctt attgtatttc cgcggatgtt tttgtggcta ccggcgccaa
     4621 gatcatgatt ttcgattgta ttgttattgt tgagtaaatt tttacgcacg gatgcatatg
     4681 ctgaagcccg actctccggt atcttgttgt taaatagaaa gtgtagcaat tggtacacca
     4741 tggaagagtg aacattgtaa agggttgtgg ctttaagata aaactttatg aaatttttaa
     4801 gctgtgtcaa caaatcaggt atattattat ttgataagct atgcgtttaa agttcgaata
     4861 tcttattatt cttgcgaaac aaaaatacaa aatgagatta ctttttgcga tgttgttctt
     4921 ctttctatgg cttgcagcag cttgtggtca gaaatacagg acggccgccg gaatcagaat
     4981 aggtacagag tttggtatta cagttcagca actgatttta gagaattcaa ccctggaagg
     5041 tattatacaa aaaggttttt ttaacgacca gacaaccata agtgtccttt ttgaacagca
     5101 tcagaaactg gttttcaagg gcttgaattt ttactttggc ggaggtcctc atgtaggatt
     5161 atatgatgca aacgatcgaa atggaagaaa gaatagtttt ggcctttcag caattggggg
     5221 cgtggaaatg cgatttggca aggtgttggc atcatttgac tataaacctg cgataaattt
     5281 tttcggtggc gaacatatct ttgacagcca atccgctatt tctctacgtt atattatcgt
     5341 tcccgctaag aaaaaggaag caaaatggaa gttttgggag aaaaagaaaa agaagaaatg
     5401 atcatccatt catttgtgac catttcttct tttcattcct atcgggtaca tatcgtgttg
     5461 atgtacaagc atcatatcta gcgcacgatc tgaatcataa gtccccgata tgccttatta
     5521 tccttaatag gcagaattaa ataattgccg gggctaagtt gggaaatatt aatttccgtt
     5581 agcgtttcat tgattttttg tcttttcaat actgtccctt tcatatcaat cagccagcat
     5641 tcgtccacct tctcatgtgc ttccacatag
//
LOCUS       DAOQQN010000205         5660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6292_length_5660_cov_4.485281, whole genome shotgun sequence.
ACCESSION   DAOQQN010000205 DAOQQN010000000
VERSION     DAOQQN010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6292_length_5660_cov_4.485281"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLG24_08350"
     CDS             <1..274
                     /locus_tag="PLG24_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMZ24941.1"
                     /translation="AIVMESHPHLLPEWEEIASTATAVENMWLMATALGIGSYWSTPG
                     IINHLQPFLSLSDNQKCIGLFYMGYTDVELPASKREDISTKVQWRE"
     gene            298..1440
                     /locus_tag="PLG24_08355"
     CDS             298..1440
                     /locus_tag="PLG24_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HMZ24942.1"
                     /translation="MNSLKQFFLLRDDVTFLNFGSFGACPRPVLEEYQRFQLEMEEEP
                     VAFIIDKSPHYLKESREALGRYLNCDGDDVVCVTNPSYAVNIVARSLDLKPGDEVLAT
                     NIEYGACDRTWRYYCKKRGAIYRQQPIRFPLEDKEDFIRQFMAGVNDRTKLIFISHIT
                     SSTALRLPVEEICALARERGIMTFVDGAHGPGQVEVDLRQMGADIYTGASHKWMMTPK
                     GSSFLYIRKGLQDLFDPIIISWGYEAVAPSSSRFLDYHEVQGTRDLSAFCTIPAAIRF
                     MEEHNWRSVASECRKLVQKNASTLTTITGHLPIAPINDDFTAQMYAAQIDTTDSQALH
                     DRLYRTFNIQIPVMAQGDQFYIRYSINGFNEQADLDKLFDALRILL"
     gene            complement(1530..2114)
                     /locus_tag="PLG24_08360"
     CDS             complement(1530..2114)
                     /locus_tag="PLG24_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HMZ24943.1"
                     /translation="MKRFMTRPFFISLILTLSGIIFLFSSTKNDTGVHQSIYDFNATT
                     LDGAPFHFADLKGKTILVVNTASNCGFTPQFESLEALYRKYADRGLVIVGFPCNQFGG
                     QEPGGKEETEKICFAKYDVTFPMMEKTDVNGRNAHPLFVFLKDRLPGFPGKKIKWNFT
                     KFLINAEGKPIKRFAPWDKPEKIDHYLENHIWKK"
     gene            2183..2362
                     /locus_tag="PLG24_08365"
     CDS             2183..2362
                     /locus_tag="PLG24_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24944.1"
                     /translation="MEGNNPAFLKLQNYTNYKKDSSPDSETEKGIDNAHGNTLRKHPL
                     FIDIEKKKVAYFHGP"
     gene            complement(2543..2791)
                     /locus_tag="PLG24_08370"
     CDS             complement(2543..2791)
                     /locus_tag="PLG24_08370"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HMZ24945.1"
                     /translation="MDWKVEDNQLRKEFRFSDFKAAMAFMQLVAFEAEFLGHHPEWTN
                     VYNTVRIRLYTHDAGNVITDRDHRLADAIDRIYTDYFA"
     gene            complement(2838..3785)
                     /gene="rsgA"
                     /locus_tag="PLG24_08375"
     CDS             complement(2838..3785)
                     /gene="rsgA"
                     /locus_tag="PLG24_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HMZ24946.1"
                     /translation="MKGIVTKSTGSWYTVRSEQGEHLECRIVGKFRLKDFELTNPVAV
                     GDEVEYKWDEVEGKGVITRIGPRRNYIVRQSPRMKHNLHLIASNIDQAVLIVTIVQPD
                     LKPGFVDRYLLMTEPHDIPAIIVFNKSDLYDEGATALLDYFRNLYENIGYETFAVSAL
                     KHDGLEALKSRLIGRTTLIGGQSGVGKSSLVNAMFSGFDLRTGEISDYSGKGVHTTTF
                     AEMYSLEEGGSIIDTPGIKTLSFNNLTPMDVAHNFRELFKMSEHCRFGGSCTHRNEPG
                     CAVREAVDKGIINEIRYQNYQAIIDELNEQNYWERKKKM"
     gene            complement(3782..4552)
                     /locus_tag="PLG24_08380"
     CDS             complement(3782..4552)
                     /locus_tag="PLG24_08380"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HMZ24947.1"
                     /translation="MSGYIGNIYNRLSEDRAHGQKRFAVLIDPDKLRMSNLDQVLKAS
                     VDAGVDYFFIGGSLIVSDVLDQLLMSIRQATNIPTVLFPGNSYQLSYKADGILFLSLI
                     SGRNPELLIGNHVLAAPYLKFSPLEILPTGYILIDGGVNTTVSYISNTRPIPANKPDI
                     ASCTAMAGEMLGLRLIFMDAGSGALNPVPAEMISAVNNVISSPLIVGGGIRTPEAARE
                     AARAGADLIVVGNAIEKDPGLIEDIASAIHTSRTISSL"
     gene            complement(4759..5040)
                     /locus_tag="PLG24_08385"
     CDS             complement(4759..5040)
                     /locus_tag="PLG24_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HMZ24948.1"
                     /translation="MNKGDLINKIADSAKITKTQAGNALDATIKAISSSLKKGDKVTL
                     IGFGTFSVSKRSARTGRNPQTGKEIKIAAKKVARFKPGKSLADTVASGK"
     gene            5327..>5660
                     /locus_tag="PLG24_08390"
     CDS             5327..>5660
                     /locus_tag="PLG24_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ24949.1"
                     /translation="MGKEEQIEKSASTEEKIKEAARRVFTRKGYAATRTRDIAEESGY
                     NVALINYYFRSKEKLFDLIMLEHLQLFVSSVHDHINDRTTSLPQKVEILISHYIDMLI
                     KNPDLPVFV"
BASE COUNT         1602 a         1307 c         1223 g         1528 t
ORIGIN      
        1 cgccattgtc atggagagtc atccccacct tctgcctgaa tgggaggaaa tcgcttcgac
       61 agcaaccgca gtggagaaca tgtggctgat ggcaaccgcc ttaggcatcg gatcgtattg
      121 gtctactccg ggtattatca atcatttaca accatttttg agtctttctg acaatcaaaa
      181 atgtatcggc ctcttctaca tgggctacac cgacgtagaa ctgcctgctt caaaaaggga
      241 agacatctct accaaggtac aatggcgtga ataaccattc actttaaaat tgaaatgatg
      301 aacagcctca aacagttttt cctactgagg gacgatgtaa ccttcctcaa tttcggatca
      361 ttcggcgcat gcccccgacc tgtcctggaa gaatatcaac gattccagct ggaaatggaa
      421 gaagaaccgg tcgcatttat aatagataaa agccctcact acctaaagga atccagggaa
      481 gccctcggcc gctacctcaa ttgcgatggt gatgacgtgg tgtgtgtcac caacccgtcg
      541 tatgcggtaa atattgtggc gcgaagcctc gatctgaagc cgggtgatga ggttttggct
      601 accaatatcg aatatggtgc gtgtgatcgt acatggcgat actattgtaa aaaaagaggt
      661 gccatatacc gacaacagcc aattcgcttc cctttagaag ataaggaaga tttcatccgg
      721 caatttatgg ccggtgtcaa tgacaggaca aaactaatct tcatcagtca catcacaagc
      781 tctacagccc tgcgtttgcc tgtggaagag atctgtgccc ttgcccggga acgcggcatc
      841 atgacctttg tggatggtgc ccacggaccc ggccaggtag aagtggacct taggcagatg
      901 ggagcggata tatacaccgg agcgtcacat aagtggatga tgacgccgaa aggctcttca
      961 tttctgtata tccgaaaggg acttcaggat ctcttcgatc cgataatcat tagctggggc
     1021 tatgaagcgg tggcgccctc ttcctcccgt ttcctggatt atcatgaagt gcagggcaca
     1081 cgtgatctgt cggccttctg cacgatacct gccgcgatac gctttatgga agaacacaat
     1141 tggcggtcag tcgcttccga atgtcgtaaa ttagtgcaaa agaatgcatc aaccctgaca
     1201 accatcacag gccatctgcc aatcgcacca atcaatgatg attttacagc acagatgtat
     1261 gccgcgcaaa tagatacaac ggatagccag gcactacatg acaggttgta tcgtacattt
     1321 aatatccaaa ttcccgtgat ggcccagggt gatcagttct atatccggta ttcaatcaat
     1381 ggattcaacg aacaagctga tctcgataag ctttttgatg cattgaggat tttattatga
     1441 gcaggattca agtcaccaac agtttttgat agtggatact gacctgcaaa aagcgaaatt
     1501 gaaacctgac aaagatcctg acaattgatc tatttcttcc agatatgatt ctctaaataa
     1561 tggtcaattt tctcaggctt atcccaggga gcaaaccttt taatcggctt gccttcagcg
     1621 ttgatcagaa acttggtaaa gttccacttg attttctttc cgggaaagcc gggaagccgg
     1681 tccttcagga aaacgaatag tggatgagca tttcgaccat tgacatctgt cttctccatc
     1741 attgggaaag ttacatcata tttggcaaag catatcttct ctgtttcttc tttcccgccg
     1801 ggttcctggc ctccgaactg attacacgga aatccgacaa taactaaacc gcggtcggca
     1861 tatttccggt acaaggcttc aagactttca aattgcggtg taaatccaca attacttgct
     1921 gtgttgacga ccaggatggt tttgcctttc agatcagcaa aatggaaagg agcgccatct
     1981 aaggttgtgg cattaaaatc gtatatgctt tggtgcacgc cggtatcgtt tttggttgaa
     2041 gaaaaaagaa aaataattcc tgataatgta aggatcaggg atataaaaaa aggtctggtc
     2101 atgaatcttt tcattatcaa tataaatgta gtcgcggcta agaaatgccg gcaaaccttt
     2161 cagtattcac acagaaagca tcatggaagg taacaatccc gcctttctaa agttgcaaaa
     2221 ttacaccaat tacaaaaagg attcatcccc ggactctgaa acagaaaaag gtatagataa
     2281 cgcccatgga aatactctcc gaaaacatcc tttgtttatt gacatcgaga aaaagaaagt
     2341 ggcttatttt catggaccgt gataataccg aaggaacatc cggtattacg ccccggaaaa
     2401 ccgagctttt cattatcggc tccgccatat caaatccacc ccattgacta aaaaaaatcg
     2461 atcggtccat aaatacatac gatttggaac aaatagacca aaggcaggcc cctgaccatc
     2521 gactttaaaa aaactcgtaa tcttaagcaa aataatcggt gtaaatcctg tcaatagcat
     2581 ctgccaaacg atgatcacgg tctgtgataa catttccggc atcatgtgtg tacagtctga
     2641 tacgcactgt attatacaca ttggtccact ccggatgatg tccgaggaat tcagcttcaa
     2701 atgccaccaa ctgcataaag gccattgctg ccttgaaatc agaaaaccgg aactcttttc
     2761 tcaactgatt atcttcaact ttccaatcca taacatgatt tgttataaaa aaatgtacgc
     2821 aaagcataca gtcaaggtta catctttttc tttcgttccc aatagttttg ctcattcagc
     2881 tcatcgatga tggcctgata attctgatac cggatctcat tgataatgcc cttgtctacg
     2941 gcttcccgca ctgcacaacc gggttcattc ctatgcgtgc aagacccgcc aaaacggcaa
     3001 tgttctgaca tcttgaataa ttccctaaag ttgtgagcaa catccatcgg cgtgagattg
     3061 ttgaaagaca gggtcttgat accgggcgta tctatgatag agcctccctc ttcgagtgag
     3121 tacatttctg cgaaagtagt tgtatgtaca cctttgccac tgtaatccga tatctcacct
     3181 gtcctgagat caaatcctga gaacatggca ttgaccaggc tgcttttgcc gactcctgac
     3241 tggcctccga tgagggttgt ccggccgatg aggcgcgatt tcagggcttc caggccatcg
     3301 tgcttcaagg cagaaacggc gaaggtttca taaccgatat tctcgtacag attgcggaaa
     3361 tagtcgagca aagctgtggc cccctcatcg tataggtcgg acttgttaaa gactatgatg
     3421 gccggtatat cgtgtggctc cgtcatgagg agatatctgt cgacaaagcc gggcttgaga
     3481 tccggctgga caatggtgac aatcaatacg gcctggtcga tattggatgc aatcaggtgg
     3541 aggttgtgct tcatgcgcgg tgactggcgg acgatgtagt ttcttcgcgg cccaatcctg
     3601 gttatgacac cctttccttc cacttcatcc catttatact ccacctcatc acctactgcc
     3661 acgggattgg tcagttcaaa atccttcagt ctgaacttgc caacgatgcg gcattcaaga
     3721 tgttctccct gttcagaccg aacagtgtac caggaaccgg tagatttggt gactatgccc
     3781 ttcacaggga ggaaattgtc cttgatgtat gaatagccga agcgatatct tcaatcaggc
     3841 ccggatcttt ttctatggca ttacccacga ctatcaggtc tgctccggcc cgtgccgcct
     3901 cccttgccgc ctccggggtg cgtataccgc ctccgacaat aagaggggag ctgattacat
     3961 tattgacagc cgatatcatt tccgcaggca ccgggtttag cgcaccgctt ccggcatcca
     4021 taaagatcag gcgtaggccc aacatctcgc cggccatcgc cgtacaactc gctatatccg
     4081 gcttatttgc cgggatcggc cgggtgttgg agatataact tacggttgta ttaacacctc
     4141 catcgatcag gatgtagccg gtcggaagga tttcaagcgg actgaactta aggtagggcg
     4201 ctgccaatac atgattgcca atcaataatt caggattgcg acctgatatc aaggacagga
     4261 ataggatgcc gtcagccttg taactcaatt gataggaatt gcccggaaat agaactgttg
     4321 gtatattggt cgcctgccga atggacatga gcaactgatc caggacatcg ctgactatca
     4381 ggctaccccc aataaagaaa tagtctacac ctgcatccac cgaggcttta agtacctggt
     4441 ccaggttgga catgcgtagt ttatccggat cgatgagtac agcgaaccgt ttctgaccgt
     4501 gtgcccggtc ttcagacaaa cgattatata tatttcctat gtatccggac atttcatcac
     4561 aaaggtatga aatttgcgac aacaatgtac ggtaaatgcc taatgacaag atcaatgaca
     4621 agaatatctg ctacgaaatc aaataagaaa cctgcttatt cattccctca aacaactctg
     4681 tcacctgggc gatctattga agataaaaaa agggcaaccc ttgcggatcg ccctttaatc
     4741 tctttcagtg tcttctgatt acttaccgga agcaactgta tcagcaaggc tcttacctgg
     4801 cttgaatctt gcaactttct tagcagcaat tttgatttct ttgccagtct gagggtttct
     4861 accagttcta gcgcttctct tagaaacaga gaaagtaccg aaaccgatca aagtaacttt
     4921 gtcacctttc ttcagagaag aagagatagc tttgatagta gcatcaagag cgtttccagc
     4981 ctgagttttg gtgatctttg cagaatctgc aatcttgttg atcaaatctc ctttgttcat
     5041 gattgaattg ttttagttat tattaaattt tttaatgagg caaagatagg cctatatttg
     5101 gtttcttgca cacttttaaa cttttatttt tgcctgcaag gtgcatattt actaaattta
     5161 gctaaacatt aacacttgaa agcctcattt aaagcagaaa ggggtagttt ttgaggtctg
     5221 aaattcaatt atagcaagcg aaacgcaaaa ttaacatttt ccatatatga aatactgtta
     5281 aaaatttatt tcctaattca aaaaattaat tttacctttg ccttgaatgg gcaaagagga
     5341 gcaaatagaa aagagtgcat ccaccgagga gaagataaaa gaagccgcgc gcagggtctt
     5401 tacccgtaaa ggttacgcag ctacacgcac gcgagatatt gccgaggaat cgggttataa
     5461 tgtagctctg atcaattatt actttaggag caaggagaag ctttttgacc ttattatgct
     5521 ggaacattta caattgttcg taagcagtgt gcatgatcac atcaacgacc gtaccacatc
     5581 attgcctcaa aaagtggaaa tactcatcag ccattacatc gacatgctga taaaaaatcc
     5641 tgacctaccg gtctttgtac
//
LOCUS       DAOQQN010000206         5657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6298_length_5657_cov_5.433238, whole genome shotgun sequence.
ACCESSION   DAOQQN010000206 DAOQQN010000000
VERSION     DAOQQN010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6298_length_5657_cov_5.433238"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLG24_08395"
     CDS             complement(<1..214)
                     /locus_tag="PLG24_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24950.1"
                     /translation="MPEISIQSTVRDLNLHLWEMSESLEWFASRTILTPPENLELESM
                     MGKRRKEFIVQRFLLQQQFGDALPILR"
     gene            1090..1989
                     /locus_tag="PLG24_08400"
     CDS             1090..1989
                     /locus_tag="PLG24_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24951.1"
                     /translation="MESIELKIKHFNSLLGSETSIYAMAQNTENNGFFTGGGSGEIVL
                     WPNFLGEEGLLYARTESPVLCLHADAVDNLLLIGTLDGQLNWIDLNKKSTVKRQKWMK
                     GGLYAIQSDEYSYYISGHGGIVTRISRDAKQATGGLQVSGIRCRALTQNSQFLISGTS
                     EGKIYMFDKLRFNNPIISSHKHQGSVFDIIQIENNYISCGKDGKLFKWDTALKKVDEV
                     QAHNSTINTLAPIGNSGLFATGCRDGSIRIWDSHDFELLKVIDLFLHSGHLRSVNKLI
                     WVDHQNLLMSCSDDRQIKMWKVQ"
     gene            complement(2132..3001)
                     /locus_tag="PLG24_08405"
     CDS             complement(2132..3001)
                     /locus_tag="PLG24_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HMZ24952.1"
                     /translation="MNSKLILSIAIFLFSFFNMSRAQVNQVKNIQLQLEGMVLDTFTL
                     EIRGSEVYINGRIADSSHVKYLLVKKDIATGNKIISEHGIEYQAEMKSAPVQIVEPPY
                     KGSPWLGLVTVQDGKGIKVVEVVKNGPASKTRILPGDYISTIDELKINNLDQLSDLVN
                     AKSPGEKIEIKLIRDETVKIVPVIIGDKNSGEIKRSYSKVDQPQMNGGRQIGMQLKLT
                     QDSKYWKVVSITSGSRAQSAGLNVNDIILEMNGVALKPGTDLAELMSEIGAGTEILLK
                     VKRAGKVLNIKMP"
     gene            3510..4178
                     /locus_tag="PLG24_08410"
     CDS             3510..4178
                     /locus_tag="PLG24_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ24953.1"
                     /translation="MFWKELVRQMYDIGIGSLVIVALICIFIGAVTAIQFNYQLDGTL
                     VPKYYIGYIVRDITLIEMAPTITCLVLAGKVGSNMASELGGMRQKEHIDAMDIMGVNT
                     YNFLIGPKVLGGVLVIPMLVTVGGFISIIGGYLASVPTGMLSNEQYIQGLNSFFVPFN
                     VTLMLIKAVVFSYILTSISCYQGYYVKGGSIELGKASTRAVVISDIAILVADYILALL
                     LATK"
     gene            4197..4955
                     /locus_tag="PLG24_08415"
     CDS             4197..4955
                     /locus_tag="PLG24_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ24954.1"
                     /translation="MIRALNIKKSFGNNLVLKGISSTFESGKCNLIIGRSGAGKTVLL
                     KILVGLLLPDEGEIWFDEINALNLNKSDTRKLRSKMGMLFQGSALFDSMSVEENIRFP
                     LDMFTTMTYKEKRARVDECLERVDLANKNKLYPSELSGGMQKRVGIARAIVLKPQYLF
                     CDEPNSGLDPKTSIVIDQLIYSITRENNITTVINTHDMNSVMEIGDTINFIDGGNLAW
                     HGIKEDVLDSDSEELQKFIFASPFLQKLKARAEK"
BASE COUNT         1833 a         1143 c          978 g         1703 t
ORIGIN      
        1 ttctaaggat gggcaaagca tcaccgaatt gctgctgtaa aagaaatcgc tgaacgatga
       61 attcctttcg cctcttaccc atcatacttt caagctctaa attttccggc ggagtcagga
      121 ttgttctact agcaaaccat tccaatgact ccgacatctc ccacaagtgg aggttcaaat
      181 ccctgactgt cgactgaatg gagatttccg gcattgataa tttaaattat agcacaaaaa
      241 tgaaaatatc ttgtgagaaa acaaacattg cttcctcaac cctattattg ttaacaacaa
      301 gacttttaat gttatatttt acatatatta tgttatttaa atatctattt atgttaatct
      361 aacaattgat aaatatcaaa acaataaaat attaataccc aaattgagca aaaaagaaat
      421 tttgaaagat gtaaaaacta ctaatccacc tgagctattt caagtaagct ggcacatagc
      481 agatgtaaat tttattaaat tggtgtaaat gaatatcaaa gaatgctcaa agacaacaac
      541 ctaatacctc atggattcct ggaaattaag gatagatagg agcactatgg gaccaattcc
      601 ggggtcaatt tgcccgaatc cttccaaaaa tcctcagcta tcacttactt accgatattt
      661 agcacctgtt tcttcggaaa ccttccaaac caatctgaat gctaagcacc aaacttctca
      721 aaaatccaag taggtagaaa aataataact gataagtaca tgccatttgg atcctctatg
      781 agtttgaaaa taaatgacgc cactttgtta caattcccgg taactatatc ttcttcttcc
      841 gcactaacac tgaccaatcg gctattactt ttctacctac ttcagttcaa ccttcctcct
      901 cacaccacat cctgatctac caatattcct atcaacccgg aaaatgccag tcatcaagaa
      961 tcatggattt aaataaattt acgcacttcc ataaacaata cccgccattt agcctttcta
     1021 aaagcataat cgatgattaa atctatctgg tagtcataca caaaattcct tgaaaatatt
     1081 tttgtcacaa tggaaagtat cgaattgaaa atcaagcatt ttaatagctt attaggcagt
     1141 gaaacatcta tttatgctat ggctcaaaat accgaaaata atggcttttt caccggaggt
     1201 ggaagtggtg agatcgtcct ttggccgaac ttcctcggtg aggagggatt gttatatgcc
     1261 aggacggaga gccctgtatt atgcctgcat gctgatgctg tcgacaatct acttcttatt
     1321 ggaacccttg acggtcaact taactggatc gatctgaaca aaaagtccac tgtcaaaaga
     1381 cagaaatgga tgaaaggcgg attatatgcc atacaaagcg atgaatatag ttactatatc
     1441 tccggacatg ggggcattgt cactcgcata agcagagatg ctaaacaggc aaccggtggc
     1501 ttgcaggttt caggtatcag atgtcgtgct ttgacccaaa atagtcaatt tctgattagt
     1561 ggaacttcag aaggaaaaat atacatgttt gataaactcc gttttaataa tccaatcatc
     1621 agttcccaca aacatcaggg gagtgtcttc gatatcatcc agattgaaaa taactatatc
     1681 agttgtggca aggatggcaa gctattcaaa tgggatactg cgcttaaaaa agtggatgaa
     1741 gtgcaggctc ataactccac aatcaataca ttggcaccaa ttggtaatag cggattattt
     1801 gctacgggtt gccgcgatgg aagtataaga atatgggatt cgcacgactt tgaactactc
     1861 aaagtcatcg atttgttctt acattccgga catctgcgct cagtgaataa attgatctgg
     1921 gttgatcacc agaacttatt gatgagttgc agtgatgaca ggcaaatcaa aatgtggaag
     1981 gtgcaataag aacaaatccc ttacaatttg gcaaacgctt aactacagta cttcctgaat
     2041 aaaattccgc ctccggtaat tctacagatt acccaatctt aatcattttg caagagacaa
     2101 aaaaccggtg tctaacccaa tcatctaatt atcacggcat cttgatattc aagaccttac
     2161 ccgctcgctt taccttgaga agtatctccg taccggcgcc tatctcactc atcaactccg
     2221 ccaaatcagt acctggcttt agcgctacac cattcatctc gagaatgatg tcatttacat
     2281 tcaggcccgc actttgtgcc cttgaacctg aagtaatcga cacaaccttc caatatttac
     2341 tatcttgagt tagtttaagt tgcatcccta tctgcctccc accattcatt tgcggctgat
     2401 cgaccttcga gtaagatctc ttaatctcac cgctattctt atcaccaatt attaccggta
     2461 cgatcttgac cgtttcatcc cgaattaatt tgatttcaat cttttctccc ggcgatttcg
     2521 cattcacaag atcgctcagc tgatctaagt tattgatttt caattcatca atagtagaga
     2581 tatagtcgcc cggcagaatc ctggttttgg aagccggccc gttctttacc acttccacca
     2641 cttttattcc ctttccgtcc tgcacagtaa ccaaacccag ccagggcgaa cccttgtatg
     2701 gcggttcaac tatctgtacc ggagcgcttt tcatttcagc ctgatactca ataccatgtt
     2761 ctgaaataat cttattaccg gtagcgatat ctttctttac aagtaaatat ttaacatgtg
     2821 aagaatctgc tatcctacca ttgatataaa cctcacttcc ccgaatctca agggtgaagg
     2881 tatccaatac cattccttcc aattgcagct ggatattttt cacctgattg acctgggccc
     2941 ggctcatatt gaaaaaacta aagaggaaga tggcgattga caagatcagt ttagagttca
     3001 taataactaa attttaatga tttaaaatgt tttcaataaa aacgcacagc gactttactc
     3061 cactaaagta ccataatctg ggatacaatc ctttaaaaaa tagttaaatt tttaaacatg
     3121 gcaaaagcga aaatccataa gcacattgag ctatgtatta caacttgtct tacaacacat
     3181 tatggccaat gccctctatt tttaccaaat aactttgaaa ggccgccatt ctattaaagt
     3241 tatcatttgt ttcattgatt aattatctaa tgtgaacttc cggttaaatc tcggaaccag
     3301 ttaaatattt ttatagatat gattttgtaa atcagaccat tatatttaaa tttaaaaaat
     3361 ataataaaat tattatatat gtaattaaaa atgaaactat atttacccga taattcttga
     3421 accaaacaat gttcaaaaat ccgctatacc atttaggaag gtacttaacc atgatgcgac
     3481 tggtaataag ccggcccaat aggttcagta tgttttggaa ggaattggtc cgtcagatgt
     3541 atgatatagg gattggctcc ctggttatcg ttgcgttgat atgcatattc ataggcgctg
     3601 tgacggcaat tcaatttaat taccagctgg atggcactct ggtccctaaa tattatatcg
     3661 gctatatcgt aagggacata acacttatcg aaatggcacc aaccatcacc tgtcttgttc
     3721 tggcaggcaa ggtaggttca aacatggcat cggaactggg aggcatgcgc caaaaagaac
     3781 acatagatgc catggacatt atgggggtca atacctataa tttcctgatt ggccccaaag
     3841 tactcggagg ggtactggtc atcccaatgc tcgtaacagt tggtggattt atcagtatta
     3901 ttggaggtta tctggcaagt gttcctacag gcatgctatc caacgaacaa tatatacagg
     3961 gtctgaattc ctttttcgta ccattcaatg ttaccctgat gctaatcaag gcggttgtat
     4021 tcagttacat tcttacatca atatcctgct accagggata ttatgttaaa ggcggaagta
     4081 tcgaattggg gaaagccagt accagggcgg tagttatcag tgacattgct attcttgttg
     4141 ccgattatat tcttgcatta ttgttagcca caaaataaaa aatagaagga ataataatga
     4201 ttcgagcttt aaatatcaaa aaatccttcg gcaacaatct tgttttaaaa ggaattagtt
     4261 cgaccttcga atccggtaaa tgtaatttga ttataggacg gagtggtgca ggtaagaccg
     4321 tattgctgaa aattctcgtc ggcttattac ttcccgatga aggggagata tggtttgatg
     4381 aaatcaacgc tcttaatctg aacaaatcgg ataccagaaa actccgaagc aaaatgggta
     4441 tgctgttcca aggttctgcc ctttttgatt caatgtcagt agaagaaaat attcgttttc
     4501 cactggatat gtttaccacc atgacctata aggaaaaaag ggcccgcgtg gatgaatgcc
     4561 ttgaacgagt ggaccttgct aataaaaaca aactctaccc ttctgaattg agcggcggaa
     4621 tgcaaaaaag agttggtata gcacgggcaa ttgttctgaa gcctcaatat ttattttgtg
     4681 acgaacctaa ttcagggctg gacccgaaaa catctatcgt tattgaccaa ctcatttatt
     4741 ccattaccag ggaaaataat ataacgactg ttatcaatac acatgatatg aattccgtga
     4801 tggaaattgg cgacacgatc aatttcattg atggaggaaa tcttgcctgg catggaatca
     4861 aagaggatgt gttggatagt gatagtgaag aattgcagaa atttatcttt gcttctcctt
     4921 tcctgcaaaa attaaaagcg agagcagaaa aataaaaaag acccgcagac tgcctttgca
     4981 atcccgggtc tgaagactaa ggtctcaaaa taagaaaact acttctttag gctggcgccc
     5041 aaattcttta tatttgaaaa aatcatatat aattaaatcc tatattatat aaccgaattt
     5101 tattcaaatt tgtttcattt atcatttaag actgccgaac atttccgaca tccttcaagt
     5161 atgcaaatat aatgccaaga ataatcagta aactaatatt gaataaatat aatatgataa
     5221 atatttaaaa atatgcagca atattatatt tattctaaat atattttcca ttataggata
     5281 ttacagtaat tattagggtc aatattttta ataattttat tttgaaaaaa cattttactt
     5341 gaaaaaaaat actgaaataa taagactgac cggtggctgt acatggggag atgtgggtaa
     5401 aaaaattaca atctttaaca tctcatttga gtccgaacaa ataatttgtc aacaaaatca
     5461 cccccaccat ttctttttga acactcctta ttcatctaaa acccgcttca acttgttcag
     5521 ttgctcgttg tagaataaac cgatcttgcc aaccaatttg ttcagccatc cataaatata
     5581 catgtcttct ctgactcagt atttcaaacc gttgccctat catttataca cgatggaaca
     5641 aaattgacag gttcatt
//
LOCUS       DAOQQN010000207         5643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6319_length_5643_cov_7.015390, whole genome shotgun sequence.
ACCESSION   DAOQQN010000207 DAOQQN010000000
VERSION     DAOQQN010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6319_length_5643_cov_7.015390"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PLG24_08420"
     CDS             complement(<1..602)
                     /locus_tag="PLG24_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24955.1"
                     /translation="MIGKALLPFVFIMAITTAGFGQFAIDVGYSGSTIINNPLRPIIH
                     SFNSYLPEKARTMRRMYYLSSIHAGIGMVDQPVNFNLEYNGTFSSNIAKKLLRTGGAE
                     YNYILNYSVNAFGPSFNFALGDKLCLGAAGLYNIISIKDYYTDTKDRTSLASSNIISV
                     KPFLGINLGSGEARVTTSLRFFANLPFKAVDITNVYSKLDP"
     gene            691..1818
                     /gene="dprA"
                     /locus_tag="PLG24_08425"
     CDS             691..1818
                     /gene="dprA"
                     /locus_tag="PLG24_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HMZ24956.1"
                     /translation="MSLNSTSYPEEEIYYGLALALIPGIGHIYAKTLLSYNGSFRQIF
                     HSKGSQLLKTPGMGKELIRRILNFTDFDKVEEELKYIQDHQISPLFYNHPDFPQRLLY
                     FNDSPFLIFRKGNAGLNSTRTLGIVGTRSPSQAGIRWVNDLISDLKDSGIAIISGFAY
                     GIDITAHRASIREGLETVGVLAGGLENIYPALHRKYIDDICRHGSIITESFSHVLPDK
                     QRFPMRNRIIAALSDGLIVVESGAKGGSIITAEFAFSYNKNLMAVPGKPCDLMSKGCN
                     ILIKTQKASMVEDSSDVFREMDWHLGNQSDKYAIQTEMFNSLSDKEQSLITFLIEKGE
                     VSVDEIIKNLDIRNSELASMILSLTLDGLIVQLPGSRLAIG"
     gene            complement(1819..2265)
                     /locus_tag="PLG24_08430"
     CDS             complement(1819..2265)
                     /locus_tag="PLG24_08430"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HMZ24957.1"
                     /translation="MNSGNMLEMMPEVQASDKVLAALHDSLEIELDTMMTRFRNNYNN
                     ALKAVNEGTMTKIKQAEVEKELQDEQARIAEYQKQAQLVVGQRRQKLLAPVIDKLNAA
                     IIEVGKEKGYSFIFDVSSGGMLYVNKSEDVSSLVASKLGLKLDDKN"
     gene            complement(2414..2623)
                     /locus_tag="PLG24_08435"
     CDS             complement(2414..2623)
                     /locus_tag="PLG24_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HMZ24958.1"
                     /translation="MFSSLLLLGLGVPEGILIFFALLLLFGGKKIPELMRGLGKGIRE
                     FNSARANIEEEIKTGMREEDKGKKE"
     gene            complement(2708..3661)
                     /locus_tag="PLG24_08440"
     CDS             complement(2708..3661)
                     /locus_tag="PLG24_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HMZ24959.1"
                     /translation="MSSRAIPLVDLSKYTQGTEEEKEQFVEALGNAFHNVGFVGVINH
                     GIPKQLVDEFYSYSKAFFALPLESKEGYEISGLAGQRGYTAFGKEHAKQSKVGDLKEF
                     FQIGQTVTDDDPIKSQYPANVRVAEMPEFTETGDSLYKAFEKAGGHLLAAIGEYLGLG
                     ADYFKAKVHNGNSILRAIHYPPITKEPESAIRSEQHEDINLITLLVGASAGGLQVLNL
                     QGEWLDVVPEADEIVVNVGDMLQRLTNKYLISTTHRVVNPPREEWHRPRLSIPFFLHP
                     RSEMDLTCLESCVTPDNPLHFEPITAGEYLNERLREIGLKK"
     gene            complement(3788..5221)
                     /locus_tag="PLG24_08445"
     CDS             complement(3788..5221)
                     /locus_tag="PLG24_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HMZ24960.1"
                     /translation="MKKLLIIALIPFLLLTSCVNSLEDYNINSKSPSKVAPDPMFAYS
                     TKVLADALNSVNVNTNVFRFYAQYLTATTYLDEPRYDMTTRTIPQALWNTLYSSTLTD
                     LNEAKKLINADNAIVPEVKANKLAAIQIVEVYTWSVLVNTFGNIPYTQALDVNNSLPK
                     YDDAKTVTADILSKLDAALASIKVDAGGFGSSDLLYGGDMSKWVKFGNSLKLKLAMVI
                     ADADAATAKTKASEAASNLSNLITSNGDNALFPYLSGAPNNNPFSNDVPPLSARQDFV
                     AANTIVDLMNNLNDPRRASYFKALADGTYKGGQYGFTSQYSAHSTISTKVSATTLPGD
                     FLDAAEVNFLLAEAVERGFISGNAEEFYNNAIRASFEYWGADGADDYLAQNGVSYATA
                     GSTWQEKIGIQKWVALYNRGYDAWTDWRRLDFPELKKPDAPNVPEIPKRLIYPIIENT
                     LNATNTKDAGAAIGGDDSATRLWWDVK"
     gene            complement(5241..>5643)
                     /locus_tag="PLG24_08450"
     CDS             complement(5241..>5643)
                     /locus_tag="PLG24_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24961.1"
                     /translation="NPVRNTLEEGGGIILPGVKADGTPNDVRVDASNSDITPFGIANN
                     PNEAFIYDASFVKLRELNLTYSFDKSLFTNSKFVKGADISLTGRNLWILYKNLPYADP
                     EDVYSAGNLSGHQGGAYPTVRTFGFNVKLKF"
BASE COUNT         1674 a         1244 c         1082 g         1643 t
ORIGIN      
        1 ggatccagtt ttgaatatac gtttgtaata tcaacggcct taaagggcag attggcaaag
       61 aagcgcaagg atgttgttac ccttgcttct ccggagccaa gattgattcc taaaaatggc
      121 tttacggata taatattgga actcgccaga gaggttctat ccttggtatc cgtataataa
      181 tccttaatgg aaataatgtt gtacaatccg gctgcgccaa ggcagagttt atctccgagc
      241 gcaaaattaa agcttgggcc aaaagcattg accgagtaat tcaggatata attatattcg
      301 gcaccacctg tacgcaacag ttttttagca atatttgaag aaaaggtgcc gttgtattca
      361 agattaaaat ttaccggttg atcaaccatc ccaattccgg catgaatgct gctcaggtaa
      421 tacatccgcc tcatggtcct cgccttctca ggaaggtagg aattgaatga atgaatgata
      481 gggcgtaacg gattgttgat aatggtcgat ccggaatatc cgacatcaat ggcaaactga
      541 ccgaatccgg ccgttgtgat ggccataata aagacaaacg gcagtaaagc ctttccaatc
      601 atatctatct tttttaaaaa aatgccggtt aaagactata atcagcaaaa ataagattaa
      661 atttgggttt taatcaccaa atgatatttc atgtcactta attcaacatc ctatccggaa
      721 gaagaaatat attatggact tgccttggca ttgatacccg gcatcggaca tatatacgcc
      781 aaaacactat tgagttataa tggtagcttt aggcagatct ttcattccaa aggttcccaa
      841 ttgctaaaga ctcccggcat gggcaaggag ctgatccgcc gaatcctgaa cttcacagat
      901 tttgacaaag tggaggagga gttgaaatat atccaagacc atcagatttc gcccttgttt
      961 tataaccatc cggattttcc acaaaggcta ttgtacttca atgacagccc tttcttaatt
     1021 ttcagaaaag ggaatgctgg gctcaactcc accagaactc taggtatcgt gggcaccagg
     1081 agtccaagtc aagcgggtat ccgatgggtc aatgacctga taagcgatct gaaggattct
     1141 gggattgcaa ttatcagcgg gtttgcttat ggtatcgata ttacggccca tcgggcaagt
     1201 ataagggaag gcctcgagac cgtaggcgtg cttgccggtg gacttgagaa tatttacccg
     1261 gcccttcacc gaaaatatat cgatgatatt tgccgacatg gatccatcat taccgagtct
     1321 ttttctcatg ttctaccgga caaacaacgg tttccaatgc gcaacagaat catagctgca
     1381 ttatcagacg gattgattgt ggttgaatcc ggtgcaaagg gaggcagtat cattactgca
     1441 gaatttgcct tcagttacaa taaaaatcta atggctgtcc ccggcaaacc ctgcgacctg
     1501 atgtcaaaag gatgcaacat cctgattaaa acccagaaag catcaatggt tgaagacagt
     1561 tcagatgtct ttcgggaaat ggattggcat ttgggtaatc aaagcgacaa gtacgcaata
     1621 cagacagaaa tgttcaatag cctttctgac aaggaacaat ccttaatcac ttttctcatt
     1681 gagaaaggcg aagtgtccgt ggacgaaata atcaaaaacc ttgatattag aaacagtgaa
     1741 cttgcttcta tgatcttatc gcttaccctt gatggcctga ttgttcagtt gccgggaagc
     1801 agattagcta taggctgatt aatttttatc gtccagttta aggccaagtt tggaagcaac
     1861 cagggagctg acatcttccg atttattgac ataaagcata cctcccgagc taacatcgaa
     1921 gataaaggaa taacctttct ccttacccac ttcaataatc gctgcattga gcttatcaat
     1981 cactggagct aaaagctttt gtcttcgttg gccgactact aattgtgctt gtttttgata
     2041 ctcagcaatt cgggcctgct catcttgcaa ttccttctcc acttcggctt gcttgatctt
     2101 ggtcatggtg ccttcattga ctgctttcaa tgcattgtta taattattcc taaatctggt
     2161 catcatcgta tccaactcta tttcaagcga gtcatgcaat gcagccaaaa ccttatcgga
     2221 agcctgaacc tccggcatca tttccaacat attgccggaa ttcatatgtc cgaacttctg
     2281 ctgactaata gatacaaatg gaatacacaa aatcagtgta aatagtaaaa tcgaattttt
     2341 aaacataata tagcttatca atattgccgg gaaacggcaa agtcaaaaat taaagaaaaa
     2401 gcaataactt caattattcc ttttttccct tgtcttcctc acgcattccg gttttgattt
     2461 cctcttcaat atttgctcgg gctgaattaa actcacgaat gccttttccc aacccccgca
     2521 tcagctcagg aattttcttc ccaccaaaca ataaaagaag tgcaaagaaa atcaaaatac
     2581 cttccggcac tcccaatccc aacaacaata gcgatgaaaa catgatcaag atttatactt
     2641 tatggttata atttaccctg taaaatgata aacgtttatt ggggtaaaaa tggttaatta
     2701 cttttcttta ttttttcaaa ccaatctccc ttaacctttc gttaaggtat tcaccggcgg
     2761 taataggctc aaaatgcaat ggattgtccg gtgtaacaca actttccaaa caagtaagat
     2821 ccatttcgct cctgggatga aggaaaaacg gaatcgagag tctcggccgg tgccattctt
     2881 cacgtggagg attaaccacc cgatgggttg tagatataag atatttattc gtcaatcgct
     2941 gaagcatatc gcccacgttg acaacaatct catccgcttc aggtacaaca tcaagccact
     3001 caccttgcag attcaacacc tgaagtcctc cggccgatgc tcctaccaac aaggtaatca
     3061 aattaatatc ttcatgttgt tcggaacgga tagcactttc gggctcttta gtaattggtg
     3121 gatagtgaat agcacggagg atactattac cgttgtgcac ctttgctttg aaataatcag
     3181 cgccaagtcc taaatattca ccaatagcag ccagcaggtg tcctcctgcc ttttcaaatg
     3241 ccttatacaa gctatcgcct gtttctgtaa actcaggcat ctcagctaca cgcacattgg
     3301 caggatattg agattttatt ggatcatcat ctgtaactgt ctggcctatc tggaaaaact
     3361 ctttaaggtc acctaccttg gactgttttg catgttcttt cccaaaagct gtataacctc
     3421 tttggcctgc aagtcctgat atttcataac cttcctttga ttccagcggc aaggcaaaaa
     3481 atgctttgga gtaagaataa aattcatcta ccaattgttt ggggatgcca tggttgatta
     3541 ccccaacaaa gccaacattg tgaaaagcat ttcccaatgc ttcgacaaat tgttcttttt
     3601 cctcttcagt cccttgtgtg tatttactta aatcaaccaa aggaatagca cgtgaactca
     3661 tttttttccg atttgaggga caaagataat aaaaatcaag ttcttagagt ctattcttaa
     3721 ctccaattgg accaaaaaaa aggtggtcga aattcgacca cctcattatt atccgttaat
     3781 acttaactta tttaacgtcc caccacagtc ttgtagcaga gtcatctcca cctattgctg
     3841 caccagcatc cttagtgttg gttgcattca atgtattttc aataattgga tagatcaaac
     3901 gcttaggtat ttcaggaacg ttcggagcat ccggcttctt gagttcaggg aagtccagtc
     3961 ttctccagtc agtccaggca tcatagcccc tattgtatag agctacccac ttttggattc
     4021 caattttctc ctgccaggtg cttcctgctg tagcataact tacaccattc tgagcaagat
     4081 aatcatcagc cccatcagct ccccaatatt cgaaagatgc tctaatcgcg ttattataga
     4141 actcttctgc attaccggaa ataaatcctc tttcaacagc ttctgcaagc aagaaattta
     4201 cttcggctgc atccagaaaa tcacccggca aagttgttgc agacaccttc gtactaatgg
     4261 ttgaatgcgc cgaatactgg ctggtaaagc cgtactgacc tcctttgtaa gttccatcag
     4321 caagtgcctt aaagtatgaa gcacgtcgag ggtcgttcaa gttattcatc aaatccacaa
     4381 tagtattcgc tgcaacaaag tcctgacgtg cactcaaagg tggcacatcg ttagaaaatg
     4441 gattgttgtt aggtgcacca ctaagataag ggaacaatgc attgtctccg ttggatgtaa
     4501 tcagattact cagattggac gcagcttcgc ttgccttggt cttggcagtt gcggcatcag
     4561 catcagcgat taccatagcc aatttcaatt tcaatgaatt gccaaattta acccacttgg
     4621 acatatcccc accgtataac aagtctgaac ttccaaatcc tccggcatca accttgattg
     4681 aagccaaagc agcatccagc ttactcagga tatcagctgt aaccgtcttc gcatcatcgt
     4741 atttaggcaa agaattgttc acgtctaaag cctgagtgta cgggatatta ccaaaagtgt
     4801 tgaccaaaac tgaccatgta taaacttcca cgatttgaat tgcagccaac ttattggcct
     4861 taacttccgg aacgatggcg ttatccgcat tgatcagttt cttcgcttca ttcaagtcag
     4921 tcaaagtaga actatacagt gtattccaaa gagcctgagg aatagtcctt gtagtcatgt
     4981 catatcttgg ttcatccaga taagtagtag ctgtaagata ctgtgcatag aatctgaata
     5041 cgttggtatt cacattcaca ctgttcaaag catctgcaag aacttttgta gaatatgcaa
     5101 acatcggatc cggtgcgacc ttagaaggcg acttcgaatt gatgttataa tcttccagac
     5161 tgttgacaca gctggtcaac aaaaggaaag gaattaaagc tattattaat aattttttca
     5221 tcatttttta atttaatgga ttagaacttc aatttaacat taaatccaaa ggttcgaaca
     5281 gtaggataag cacctccttg gtgtccggaa aggttacccg cgctatatac atcttcagga
     5341 tcggcatatg gtaggttctt ataaagaatc caaaggttac gtcctgtcaa tgaaatatcg
     5401 gcacctttta caaacttgga gttggtaaac aagctcttgt caaaagaata agttagattc
     5461 aattctctca atttaacaaa tgatgcatca tagataaatg cttcgttcgg gttgttagca
     5521 ataccgaatg gagtaatatc agagttgctg gcatccactc tcacgtcatt tggtgttcca
     5581 tcagccttaa cacctggaag gataattcca ccaccttctt caagtgtgtt tctaactggg
     5641 tta
//
LOCUS       DAOQQN010000208         5635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6336_length_5635_cov_5.707348, whole genome shotgun sequence.
ACCESSION   DAOQQN010000208 DAOQQN010000000
VERSION     DAOQQN010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6336_length_5635_cov_5.707348"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..823)
                     /locus_tag="PLG24_08455"
     CDS             complement(128..823)
                     /locus_tag="PLG24_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24962.1"
                     /translation="MAFRIFILLLFCSVAVSAQEVIVREEKIIRDGFHIAPVGFTSKG
                     YTVGVGYSRPIAPALMLHGTAGYMLSSYFEQEECSASGVQITPSLLYFPDNSDNYKGI
                     CLGLELPIFYYKVSRSWWVSNTSLNEISTFEYQKYHTANATAIQGGVAARFGFRTHKL
                     NHKFFWQPNISGGILFQKLNGYKEQYNYSSDNLPLNESLHRQQSGMAPYFRFEIAFGL
                     YRYKAETVKVIEM"
     gene            complement(823..1566)
                     /locus_tag="PLG24_08460"
     CDS             complement(823..1566)
                     /locus_tag="PLG24_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24963.1"
                     /translation="MKNLVFTLFVLALSACSLKDGGFFDSGSSGNSKSGSTATILTYG
                     NYMYALDNNKLRTFDLVDPAHPQQTSQQTISATAETLFAYDNFLYIGTTTGVLMYSLA
                     DKSNPRFQNTVFHWPARDPVVVEGNYAYSTTRWGDEFQNPSGRLTVLDVKDKANAFVI
                     GTYDQKFPFGLGVSNGYLYVCNGHFGLNIFRIINGGQQLEFVKNIATDDIYDCIPSEE
                     LLVCQTKTGIWVFNIENPGDPVLTHKINN"
     gene            complement(1586..2122)
                     /locus_tag="PLG24_08465"
     CDS             complement(1586..2122)
                     /locus_tag="PLG24_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24964.1"
                     /translation="MMNRLFYFIVLIIFAAGFISCDFDEAGPPSSVRGYAPVYFPKTN
                     NDTLKFVGPQPTEKAGKIVLKGDLLYQVEQYKGIHIIDFRDANNAKKIGFYEIFGCTE
                     LTIKDHYMYTNSGTAFIVIDIADLFNPRVVDYRPDYWVGLSFPPPPGNGYFECPDPQK
                     GIVISWSETILHNPKCKL"
     gene            2892..4529
                     /locus_tag="PLG24_08470"
     CDS             2892..4529
                     /locus_tag="PLG24_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HMZ24965.1"
                     /translation="MMNKVLKLDPKDNTLVALTNLHKGETVILDGRETVLVDDIPAKH
                     KFFEDNMKAGSEVYMYGVLVGKVQTDILKGMRMTVENTKHAADPYHYRPYHYQWRGPD
                     VEKFRHRTFNGYHRSNGEVGTANYWLFIPTVFCENRNLTLIREALYNALGYAVDDKYK
                     NYAEALLKAFQAGEDLNKVALSNITPGANRPFRHVDGIKFLIHQGGCGGTRQDANTLS
                     RLLAAYANHPNVAGVTVLSLGCEHLQSSQLKDDIFALDPNFDRPLIFFNQQKSECEES
                     LIQRAILETFHGLMEINKIERKPAPLSKLCIGVKCGGSDGFSGISANPAVGHCADLLV
                     ALGGKVLSAEFPELCGVEQELLDRMPDRDVAEKFIRLMTEYDDLAHKVGSGFYMNPSP
                     GNIRDGLITDAIKSAGAAKKAGTAPITDVLDYTEPARKPGLSLVCTPGNDVEATTGKA
                     ASGATLILFTTGLGTPTGNPVCPTIKLATNSALARRMSDIIDIDTGSIIDGSKTIEQM
                     GEEILEYCIRAASGEVTPKAVQLNQDDFIPWKRGVSL"
     gene            4565..5329
                     /locus_tag="PLG24_08475"
     CDS             4565..5329
                     /locus_tag="PLG24_08475"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HMZ24966.1"
                     /translation="MFSLNNKKAVITGGGSGIGQAICVLFARQGAHVFVLELTEEAGR
                     ETRAMITDEGGTAEIIACNVAIQQEVDRAFTSIGTVDILVNNAGIAHIGKADTTSEAD
                     FDRVMSVNVKGVYNCIHAAIPRMREVGGGTILNMASIAALVGIPDRFAYSTAKGAVKT
                     MTMSVAKDYLADHIRCNSISPARVHTPFVDGYLRDTYPDNIQEMFEKLSATQPIGRMG
                     TPMEVAALALFLCSDEGSFITGCDYPIDGGFTTLNN"
     gene            5339..>5635
                     /locus_tag="PLG24_08480"
     CDS             5339..>5635
                     /locus_tag="PLG24_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HMZ24967.1"
                     /translation="MKLVRYGVAGHERPGVFIGEDIFDASILAADYDETFFANEGLER
                     LKKAADEGLLKKVGADVRLGPPIARPSKLICVGLNYRDHAAETNAKIPAEPILFF"
BASE COUNT         1541 a         1285 c         1297 g         1512 t
ORIGIN      
        1 tcttgttaag gcaaggtata tcttcccgtt ccaatgacct ccggtttttg tatgtgtacg
       61 ccatttacct gtcaaataaa ggccctacat catttttgaa taggtgaata attacacccc
      121 ccggctccta catctcgatt accttgactg tttccgcctt ataacgatac aggccaaagg
      181 caatctcaaa cctgaaatac ggtgccatac cggattgttg cctatggaga ctttcattca
      241 aaggaagatt atccgatgaa tagttgtatt gttctttata tccattcagt ttttgaaaca
      301 ggatacctcc ggaaatattg ggttgccaaa aaaatttatg attcagttta tgggtgcgaa
      361 acccaaatct ggctgccact ccgccctgta ttgcggtagc atttgccgtg tgatacttct
      421 gatattcaaa ggtcgatatt tcattgagtg atgtgttgct tacccaccag gaccgactga
      481 ctttataata gaatatcgga agttccaggc ccaggcatat tcccttataa ttgtcgctat
      541 tgtcaggaaa atacaacaac gatggtgtta tctgtactcc ggatgccgag cactcctctt
      601 gttcaaaata actgcttaac atatagcccg cggtaccatg cagcatcagg gctggtgcaa
      661 ttggcctgga atacccgaca cccaccgtat aacctttgga agtgaagcct accggggcaa
      721 tatgaaaacc atccctgatg attttttctt ccctgacaat gacctcctga gcgctgaccg
      781 caacggaaca gaacaaaagc aggatgaaaa tacgaaaagc catcaattgt tgattttatg
      841 agtcaggaca gggtcccccg gattctcgat attgaagacc cagatgccgg tttttgtttg
      901 acataccaac agctcttcgc ttggaataca atcgtagata tcgtcggttg caatattctt
      961 gacaaattcg agttgttgac caccattgat gattctaaag atgttcagtc caaaatggcc
     1021 attacataca tagagataac catttgaaac acccaggcca aatgggaatt tctgatcata
     1081 ggttccgatg acaaaagcat tggctttgtc tttgacatcc agcactgtaa gtcttccgga
     1141 tggattctga aattcatcac cccatcttgt tgtggagtat gcataattcc cttcgacaac
     1201 gacagggtcc cttgccggcc agtggaatac tgtattttga aatcttggat tcgacttgtc
     1261 tgccagacta tacatcaata cgccagttgt ggtgccgata tacaggaaat tatcataagc
     1321 aaacaaggtt tcagcagttg cagaaatagt ttgttgtgat gtttgttgtg ggtgtgctgg
     1381 gtctaccagg tcgaaagtcc tgagtttgtt gttgtccaag gcatacatat agttgccgta
     1441 agtcagtatt gtggctgtcg atccggattt gctgttgccg gatgaaccac tgtcaaaaaa
     1501 tccaccatct ttcagtgagc atgctgacag tgcaagcaca aacagggtaa acacgagatt
     1561 tttcatagta ttatatttta aaaatttaca atttacattt aggattgtgc aatattgttt
     1621 ccgaccagct aattacaata cctttttgag ggtcgggaca ctcgaaatag ccattgcccg
     1681 gaggtggcgg aaatgaaagt cccacccagt aatccggcct gtaatctacc acacgcggat
     1741 taaacaggtc tgcaatgtct atgacgataa atgccgtgcc tgaattagtg tacatataat
     1801 gatccttgat ggtcagttca gtacatccga agatttcata gaaaccgatc ttcttggcat
     1861 tgtttgcatc ccggaaatca attatgtgta tgcccttgta ttgctccacc tggtacaaca
     1921 aatctccttt caacacaatc tttccggctt tttcagtagg ctgaggaccg acaaatttca
     1981 aggtgtcatt gttggtcttc gggaaataca ccggggcata tcccctaacc gatgacggcg
     2041 gtcctgcttc atcgaagtca caggaaataa atccggcggc aaaaatgatc aacacaataa
     2101 aatagaatag gcgattcatc ataattgaat gataattggt ttcaagattt tttgtcccat
     2161 tacacagaaa tatttaccat gtaatattac taacgaagat agcaactaat tggttggtat
     2221 ccaaaaattt tatccatccg gattattttt taacaaaata ttaatgcccg gcttatttcg
     2281 aggctctaca tccgatcaag tcaggttggt ccatttcact atactttagg acatcctttc
     2341 atcggtttgc agctgcccgg ggtggcgatt ccgaagctat acactgtcaa gccagataca
     2401 aattttgatt gttgcacaaa gcttgggtta accctttttc tttatttgtt tcgggaatct
     2461 gtcaaatttt tatattcagt tcaagtcgtc cgccaaagcc aagttcaatg ttcttttaag
     2521 tgtcgaaatt cgcccttctt ttaaattatt ctcaaccttt caactgtagg atttagttgt
     2581 cccacctttt tctgcacgtt gttcctgtgt cattttcatt tcaaatacga gtgtcgtgca
     2641 taaaaacacc ggtttaaatg cttcataatc cggttaagcg gcttgaatgt tcgccccgag
     2701 taatggcgtg agaaggtctc tatgctttgc tcctacttgc ttttgttggt cgtgttgtgg
     2761 cctgttggca agctgcggag caagtcattt cttagtaatt cattctcccc ctgttaaaga
     2821 aattaatttt gactaaattt gcttctgcct gccggcctgc aagagaagtt ccggctaaac
     2881 ttctttattt gatgatgaat aaagttctga aattagaccc taaagacaat accttagtgg
     2941 cattgaccaa cctacacaaa ggcgaaaccg tcatcctgga tggtcgtgag acggtgttgg
     3001 tggatgatat tcctgcaaaa cacaagttct ttgaggacaa catgaaagcc ggaagtgaag
     3061 tctatatgta cggagttctt gtgggcaaag tacagacgga tatcctgaag gggatgcgca
     3121 tgacggtcga aaacacaaaa catgccgctg acccatatca ttacaggcct tatcactatc
     3181 aatggcgggg gcctgatgtt gaaaagtttc gccacagaac tttcaatggt tatcaccgta
     3241 gcaatgggga ggtcggcact gccaattatt ggttatttat cccgacggtc ttttgcgaaa
     3301 acagaaacct gacgctcatc cgcgaggctt tgtataatgc tctgggctat gcggtggatg
     3361 acaagtataa aaactatgca gaggcactac tcaaggcttt tcaggccgga gaagacctga
     3421 ataaagttgc cttatcgaat ataactccag gggccaatcg tcctttcagg catgtggatg
     3481 gtatcaaatt cctgatacac cagggaggat gtggcggtac acgtcaggat gccaataccc
     3541 tgagcaggct gctcgccgcc tatgccaatc accccaatgt tgcaggtgtg accgttctga
     3601 gcttgggctg cgaacacctg caatcttcac aactgaaaga cgatattttc gccctcgacc
     3661 ccaattttga caggccgttg atcttcttta atcaacaaaa gagtgagtgt gaagagtccc
     3721 tcattcaacg agccatcctc gaaaccttcc atggactgat ggaaatcaac aaaattgaac
     3781 ggaaaccggc gcctctgagc aaattgtgca tcggtgtgaa atgcggtggc agcgatggat
     3841 tcagcggtat ttccgccaat ccggccgttg gtcattgtgc cgacttgctg gtagcgctcg
     3901 gaggcaaggt tctttcggca gaatttccgg aactgtgtgg tgttgaacag gaattgctgg
     3961 accgtatgcc tgacagggat gtcgctgaaa aattcatccg gctgatgacg gaatatgacg
     4021 atctggcaca taaagtcggc tccggatttt acatgaaccc ctcaccgggc aatatccgcg
     4081 acggattgat tacagatgcc atcaaatcgg ccggagcagc taaaaaagca ggcaccgcac
     4141 cgattacgga tgtactcgat tatactgaac ccgccagaaa gcccggcctc agtctcgttt
     4201 gcactcccgg caacgatgtt gaggcgacca ccggcaaggc agcctccggg gcgaccctca
     4261 tcctgttcac taccgggctg ggcacaccta caggcaatcc ggtttgccca accatcaagc
     4321 tggctaccaa ttccgctttg gcacgcagaa tgagcgacat catcgacatc gacacaggaa
     4381 gcatcattga tggtagtaag accatcgaac aaatgggtga ggaaatcctc gaatattgca
     4441 tccgggccgc aagtggtgaa gtgactccca aggccgtcca attgaatcag gatgatttta
     4501 tcccctggaa aagaggcgta agcctataaa ataagcaagg cattcatgtt ttaaacaaaa
     4561 gaaaatgttt tctcttaata ataaaaaagc ggttattacc ggtggcggct ccgggatagg
     4621 ccaggctata tgtgtgctgt ttgcacgtca aggcgcacac gtgtttgttc tggagttgac
     4681 ggaggaggcc ggcagagaaa ccagggccat gataacggac gaaggcggca cagcagaaat
     4741 cattgcctgc aatgtggcga tacaacaaga agtggaccgc gccttcacca gcattggcac
     4801 ggtcgatatc ctggtcaata atgccggcat cgcacacatc ggcaaggcgg ataccacaag
     4861 cgaagcggac tttgatcggg tgatgtcggt caatgtcaag ggagtatata actgcatcca
     4921 cgccgccatc ccccgaatga gggaagttgg tggaggcacc atcctcaaca tggcttcaat
     4981 agcggcattg gtaggcattc cggatcgatt tgcctacagc acagccaagg gagcagttaa
     5041 gacaatgacg atgagtgtgg ccaaggatta tctcgctgat catatccgat gcaattccat
     5101 ctcacccgca cgcgtccaca caccttttgt ggacggttat ctgagagata cctatccgga
     5161 taatatacag gagatgtttg aaaaattatc agcaacccag cctattggca ggatgggaac
     5221 tcctatggaa gtggcggcac tggccctgtt cctgtgcagc gatgaaggca gcttcatcac
     5281 cggatgcgat tatccgatcg atgggggatt tacaacatta aacaattaaa accagccgat
     5341 gaaacttgta agatacggtg tagcaggaca tgagagaccc ggtgtgttta ttggtgaaga
     5401 tattttcgac gcttcaatcc ttgccgcgga ttatgatgaa acattctttg caaatgaagg
     5461 cctggaaagg ttgaaaaagg ctgccgatga aggcttgctg aagaaagttg gagccgatgt
     5521 tcgacttggt ccgccaattg cacgaccgtc aaaactgata tgtgtcggct taaactatcg
     5581 tgaccacgcg gcagagacca atgcaaagat acccgcagag ccaatcctct ttttc
//
LOCUS       DAOQQN010000209         5635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6337_length_5635_cov_5.426703, whole genome shotgun sequence.
ACCESSION   DAOQQN010000209 DAOQQN010000000
VERSION     DAOQQN010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6337_length_5635_cov_5.426703"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..1440
                     /locus_tag="PLG24_08485"
     CDS             388..1440
                     /locus_tag="PLG24_08485"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HMZ24968.1"
                     /translation="MIKRSFIIISLAFMSIMTGSTQTIMHRIAIGSNLDQEGSQRIID
                     LIARRKPDAFISLGNIITGGSANQVEMREKYGTLSEWQGLKKLKESTKFFATWDDHEY
                     GESEGGKHFSDKGAYKQLFLDFAGDPDNSLRKSHEGVYNAYGYGNAGSRVSIILLDTR
                     TFRDNLLPYQGDRRDDPRFFHPLQYSPYKSADSTLLGEDQWIWLERQLMEPALVHLIV
                     SPIPFSAEYNGMESWANFPHEQNRLFKLLDKTRALGVVFLSSHAGYSEISKLKRDGYY
                     PIYDYTCAGLNTQKYFATANANRIEGPIMEPNFGLLTFDWAPGKVTMTMECIDANNNS
                     RFEYIVKLTHLWPDSE"
     gene            complement(1517..2089)
                     /locus_tag="PLG24_08490"
     CDS             complement(1517..2089)
                     /locus_tag="PLG24_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HMZ24969.1"
                     /translation="MKHLAIFASGGGSNAQAIMKHFRNHPTIQIALVLYNKKDAGVKN
                     HAAHFGIPARFWSGKAMADQERTESLLGEYRITGIILAGYLSLIPEYLINQFPDKILN
                     IHPALLPSFGGKGMYSHFVHEAVSKAGHIETGITIHLVNKEYDKGRILFQKAISIRPH
                     EDSGVIARQVLKIEHQHYAHVIEDYFESLH"
     gene            complement(2105..3031)
                     /locus_tag="PLG24_08495"
     CDS             complement(2105..3031)
                     /locus_tag="PLG24_08495"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HMZ24970.1"
                     /translation="MKRMIMRCTFLIALLLIFFSFKGFSQEFAFTVKVNTPTLQIADP
                     GIFKNFEKSILEFLNNQRFTDMDLRDEEKIKVTLNFNLVKEQSEKDFVFSLAILAVRP
                     VYGSSYESVIFQYVDDKVVFSYEPGNALIFNPNSYNDNLTSILGYYAYIILGLDRDSF
                     AEKGGDQMFVNAQTVIGSLPTGLSNGDEYGWGANKAGLNKSRSSFVENLLSPKLNDFR
                     IGFYEYHRQGLDLASTNIDEARKNIINAMKSIEKSYDTYTNSIEINSFGTVKGNELVE
                     IFKPASKAEKQDIYKIMSKLDPAGLQKYIDLR"
     gene            complement(3028..4236)
                     /gene="coaBC"
                     /locus_tag="PLG24_08500"
     CDS             complement(3028..4236)
                     /gene="coaBC"
                     /locus_tag="PLG24_08500"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HMZ24971.1"
                     /translation="MELQGKKIVLGICGSIAAYKAAFLCRLLIKKGAEVRVVMTPSAS
                     DFITPVTMSTLSNHSVYNSIHDGNTWAGHVDLGLWADLILIAPASANTIAHMANGICQ
                     TMLDAVCLSAKCTVCVAPAMDLDMWKHPATVANINRLRSFGTQIIPVEHGFLASGLIG
                     DGRMAEPEHILAYVIDYFNRGKDLLGQKVLITAGPTHEKIDPVRFIGNASSGKMGIAL
                     AFECLRRGATVDLVLGPASENPAAALGMTIHHVTDSEEMYLKCFNLYPDTDIAILAAA
                     VADYRPIEPSEQKIKKTEDTLSITLQKTKDIAAELGRIKPAGKIHVGFALETDNELEY
                     AIAKRKRKNFDLIVLNSTRDKGATFGSDNNKVTILGESGIIRKTELLPKTEIATIIID
                     ETIKLLKKSL"
     gene            complement(4279..4614)
                     /locus_tag="PLG24_08505"
     CDS             complement(4279..4614)
                     /locus_tag="PLG24_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HMZ24972.1"
                     /translation="MTDIKTKVQGIDPNSHARNIRGMASKTGNIYEAICVISARARQL
                     QVGIKRELNSKLDEFTVATDTIEEIQENKEQIEISKFYEKLPNPAIIATEEFINGELS
                     FRYKEEHQD"
     gene            complement(4574..5452)
                     /gene="bamD"
                     /locus_tag="PLG24_08510"
     CDS             complement(4574..5452)
                     /gene="bamD"
                     /locus_tag="PLG24_08510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HMZ24973.1"
                     /translation="MKNKYLHYLCALMMRKHSLVLILLAMMAFASCKSEYEKLRASSD
                     VALVYKKALEYFSKKDYYKAQNLLEQILGRYRGKKEAEDLYVKYAYTHYYQGNYILAA
                     SYFQNFANTYLTSSLREEAAYMSAYSNYLQSPDYKLDQTYTGKAIDELQIFVNTFPKS
                     ERVKEANKLIDELRAKLEKKAFEEGMLYYNLKQYQSATITFNNMLKEFPDSKNVEKIR
                     YFIVKSNFTLAENSIYAKKLVRYQETLLSAELFKEKFPRSKDRKEIDSILKETKIHIK
                     KLENDGHQDKSAGNRS"
BASE COUNT         1617 a         1199 c         1119 g         1700 t
ORIGIN      
        1 gccacaatga acctacccgt acctttgagc tgatttcagg atcctgggca tcaaaccgaa
       61 gcgtccaaaa cgaattgcac ccttctccta tcccgaaaaa aattttgtgc tgcgaacaac
      121 aagttgaatt tagctcaata gacaataata aacctcatcg gttggtacta ttcctactta
      181 tcactaaaaa tggacccatg aatatccggg caagttcttc agaaaacaac atgattaggt
      241 tgacaaattt taacagagtg ataattttac aacgaaccca atataagtta ttatatatat
      301 taaggttaaa tataaaatat aatattaatg caatgacaag aaattcaacc ggatagtcgg
      361 gtcaggataa atttatattt ttgacctatg ataaagagaa gttttattat tatctccctg
      421 gcttttatgt caattatgac cgggagtacc caaacaatca tgcacaggat agccatagga
      481 agcaatctgg atcaggaggg ttctcaaaga attatcgatc tgatagctcg caggaaaccc
      541 gatgcattca tttctctggg aaatattatt acaggtggaa gtgcgaatca ggtcgaaatg
      601 cgcgaaaaat acggtacttt gtcagaatgg caaggcctaa aaaaattgaa ggaatcgaca
      661 aaattttttg ctacatggga tgaccatgaa tatggcgaat cagaaggtgg caaacacttt
      721 tctgacaaag gagcatacaa gcaacttttc ctggacttcg caggtgatcc ggacaactcc
      781 ctgcgcaaaa gccatgaagg tgtctataat gcttacggct acgggaatgc cggctctcgc
      841 gtcagcatca ttttacttga tacccggaca tttcgcgaca atcttttgcc gtatcagggt
      901 gatagaagag acgacccaag attcttccat cctttgcaat attctcccta taaatctgct
      961 gacagcacac ttctgggtga agatcaatgg atttggctgg aaagacaatt gatggaacct
     1021 gccctggttc acctcattgt atcacctata ccgttcagtg cagaatacaa tggcatggaa
     1081 agttgggcaa atttcccgca tgaacaaaac cggttgttta agctccttga taagacaagg
     1141 gctcttggtg tagtcttctt atccagtcat gccggttact ctgaaatcag taaattaaaa
     1201 agggacggct actaccccat ctacgactat acctgtgccg ggctcaacac gcaaaagtat
     1261 tttgctactg ccaatgctaa caggattgag gggccgataa tggagcccaa ctttggtctg
     1321 ttgacattcg actgggctcc aggcaaggtc actatgacca tggagtgcat tgatgccaac
     1381 aataacagtc ggtttgaata tatagttaaa ctcacacatc tttggccgga cagtgagtaa
     1441 tctgaagtta ctaaatgcat ctgccgaata gaactaagtt atcgacaggt gattcgcgac
     1501 tctggtaaaa caaattttag tggagggatt cgaaataatc ctcaatgacg tgggcatagt
     1561 gttgatgctc gattttgaga acttgcctgg caatgacccc ggaatcttcg tgcggtctta
     1621 tggagattgc cttttgaaac aagattctgc ccttgtcgta ttccttattg accaaatgaa
     1681 tggttatacc ggtctcaatg tgaccggctt tacttacagc ttcatgaaca aagtggctat
     1741 acataccctt accaccaaag gaaggtagta gtgccgggtg aatattaaga attttgtccg
     1801 gaaattgatt gataagatat tcaggaatca aggaaagata tccggccaaa atgatacctg
     1861 taatccggta ttcaccaagt aggctttcag tcctttcctg gtcagccatt gcttttccgg
     1921 accaaaaacg agccggaata ccgaaatggg ccgcatggtt ttttaccccg gcgtcctttt
     1981 tgttataaag aaccaatgca atttgaatgg ttgggtgatt ccggaagtgc ttcatgatgg
     2041 cttgagcatt gctgccgcct ccggaagcaa agatggcgag gtgcttcatc gtacccatat
     2101 catttcaacg aagatcaata tacttctgaa gacctgccgg atctaactta gacataattt
     2161 tgtaaatatc ttgcttttca gcttttgatg caggtttaaa aatctccacc agttcattcc
     2221 cttttacggt accaaatgag tttatttcga ttgagttggt atatgtatca taagatttct
     2281 caatactttt catggcattg atgatattct tacgagcttc atctatattg gtcgaagcca
     2341 gatcaagccc ttgtctgtga tattcataaa atccaatcct gaaatcattg agtttgggac
     2401 tcagcaaatt ttccacaaaa gaagaacgcg acttattcaa tccggcttta tttgctcccc
     2461 atccatattc atccccgttt gacagcccgg taggtaatga gcctatcacg gtctgggcgt
     2521 tgacaaacat ttgatcgccg cctttttccg caaaggaatc ccggtccagg ccgagaataa
     2581 tatatgcata ataaccaaga attgaagtca ggttgtcatt gtacgaattc ggattaaaaa
     2641 tcaatgcatt acccggttcg taagaaaaca caactttgtc atcaacgtat tggaaaatga
     2701 cggattcgta actgctgcca tatacagggc gaacagcgag aatggcaagg ctgaaaacaa
     2761 agtctttttc agactgctcc tttaccagat taaaattgag ggttaccttg attttttcct
     2821 catcacgcaa atccatatcc gtgaaccgtt ggttgttgag aaattccaaa attgactttt
     2881 caaagttctt gaaaatcccg ggatcggcta tctgcaaggt gggtgtattg accttaaccg
     2941 taaaagcgaa ttcctgactg aatcctttaa aactaaagaa tatcagcaat aaagctatta
     3001 aaaaagtaca tctcatgatc atacgcttca taacgatttt tttaataatt tgattgtttc
     3061 atcgataatg attgtggcaa tttctgtttt aggcaacaat tctgttttcc gaattattcc
     3121 gctctctcca aggatggtaa ccttattgtt gtcactgcca aaagttgcgc ccttatctct
     3181 tgtagaattc agtacgatca ggtcaaaatt ctttcgcttt ctttttgcta tcgcatattc
     3241 caattcatta tcagtctcaa gggcaaaacc gacatggatc ttaccggcag gttttattct
     3301 gcccaattca gctgcgatgt ccttcgtttt ttgcaatgta atggaaaggg tatcctctgt
     3361 tttctttatt ttctgttctg acggctctat cggtcgatag tcagcaacag cagcggccag
     3421 aatggctata tcggtatcag ggtacaaatt gaagcatttg aggtacatct cctcagaatc
     3481 tgttacatga tgtatagtca tcccaagcgc agcagccgga ttttctgatg caggaccaag
     3541 gacgagatca actgtagctc ctcttcgcag gcattcgaat gcaagggcta tacccatctt
     3601 accgcttgaa gcattgccaa tgaatctcac agggtcgatt ttttcgtgtg tcgggcctgc
     3661 agtaataagt actttttggc ctaagagatc ctttccccgg ttgaaataat cgatgacata
     3721 tgccaaaata tgctcaggct cggccatcct gccatcacca atgagccctg acgcaaggaa
     3781 tccatgttct accggaatga tttgggtgcc gaagcttcgc aatcgattga tattggcaac
     3841 tgtcgcgggg tgtttccaca tatcgagatc catggccgga gctacgcata ccgtacattt
     3901 ggcactaaga caaacagcat caagcatggt ttgacaaata ccgtttgcca tatgggctat
     3961 ggtgttggct gatgccggag caataagaat caaatccgcc cacagcccca aatcaacatg
     4021 cccggcccag gtgttcccat catggatgga attatacacg gaatgattgc taagcgtgga
     4081 catggtaaca ggtgtaataa aatcagaagc agaaggggtc atcacaaccc tgacttctgc
     4141 tcctttcttt ataagcaatc tgcacaggaa agccgcctta taggctgcta tactgccaca
     4201 tatgcccaat actatttttt taccctgcag ttccattttt aataggaatt ggcaatcagg
     4261 caaggcctat ccgggaaatc aatcctgatg ctcttcttta taccgaaatg aaagttctcc
     4321 gttgataaat tcttctgtcg caattatcgc cggattaggc aacttctcat agaactttga
     4381 tatttctatc tgctctttat tctcctgaat ttcttcgatt gtatcagttg caactgtaaa
     4441 ttcatccagt ttgctgttta gttcccgctt tatgccgacc tgaagttgac gtgctcttgc
     4501 agaaataaca cagatcgctt cgtagatatt tcctgtcttt gaagccatcc ctctgatatt
     4561 tcgtgcatga ctattaggat cgattccctg cacttttgtc ttgatgtccg tcattttcca
     4621 gttttttaat atgaattttt gtttctttga gtattgagtc tatctctttt ctgtccttgc
     4681 ttctaggaaa cttctcctta aacaattcag cggacaataa agtttcctga tatcgtacca
     4741 gcttttttgc gtaaatactg ttttccgcaa gagtaaaatt agattttaca atgaaatacc
     4801 tgattttttc aacgtttttg gaatcaggaa attccttcaa catgttattg aaagttatcg
     4861 tggccgattg atattgtttc agattatagt atagcatacc ttcctcaaac gccttctttt
     4921 caagcttggc acgcagttcg tctatcaatt tattggcctc ttttactctc tcgcttttcg
     4981 gaaaagtatt cacgaagatc tgtaattcat caatcgcctt gccggtatat gtctgatcaa
     5041 gcttatagtc aggactttga aggtaattgg agtaagccga catataggca gcttcctctc
     5101 ttagagagct tgtcagatag gtattggcaa agttttgaaa ataagatgct gccaggatat
     5161 aattcccctg gtaataatgg gtatatgcat actttacata caggtcctca gcttcctttt
     5221 ttcctctata gcgaccaagg atttgctcca gcaggttctg cgccttgtaa taatccttct
     5281 tggaaaaata ttccagtgct tttttgtaaa ccaatgctac atcactgctg gcccgaagtt
     5341 tttcatactc cgacttacag gatgcaaagg ccatcatagc aagtaaaatc aggacaagtg
     5401 aatgctttct catcataagg gcgcaaagat aatgcaagta tttgtttttc aaagtaaaaa
     5461 ttatttgatt cagaaagtac cttagtatca acgaaaaaat ttccggctct gatgcctata
     5521 agtaaacata atgtgccaaa attgcttaaa tggccattag aatgtatgct atttcaaaaa
     5581 taggttcatg atgacagata caatcaacat ccttacaatg caggttttaa gaatc
//
LOCUS       DAOQQN010000210         5616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6369_length_5616_cov_5.736558, whole genome shotgun sequence.
ACCESSION   DAOQQN010000210 DAOQQN010000000
VERSION     DAOQQN010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6369_length_5616_cov_5.736558"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..1075)
                     /gene="ruvB"
                     /locus_tag="PLG24_08515"
     CDS             complement(26..1075)
                     /gene="ruvB"
                     /locus_tag="PLG24_08515"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HMZ24974.1"
                     /translation="MLSIIYIVQNELLNSDTSHLSKEDKIIEKTLRPGYLGDFKGQRK
                     IVDNLEVFIKAAKLRGEALDHVLLHGPPGLGKTTLSNIIANELGTNLKVTSGPVLDKP
                     GDLAGLLTNLEPNDVLFIDEIHRLNTVVEEYLYSAMEDYKIDIMIDSGPNARSIQLAL
                     NPFTLIGATTRQGLLTAPMRARFGISFHLDYYDIPTLEGIIKRAAGILNVRTERNGIH
                     EIARRSRGTPRIAYALLRRVRDFAQIKGEGVITLDIARMGLNALNVDESGLDEMDNRI
                     LRSIIEKFNGGPVGISTISTAVGEESGTIEEVYEPFLIMSGFIQRTPRGRIVTRKAYD
                     HLGIKPGPIQPSLFD"
     gene            1812..3242
                     /locus_tag="PLG24_08520"
     CDS             1812..3242
                     /locus_tag="PLG24_08520"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HMZ24975.1"
                     /translation="MKVLHVAAECYPAAKAGGLGDVVGALPKYLNRIGMQAGVIIPKY
                     GTKWLWEHQFKTIFTGTVRLNRSYIRYSIEQEVDDILGFTLFAVHVPGMFDRPGVYAD
                     PTTGYGYSDELERYLVFQQAVLQWLISSAERPNVIHCHDHHTALIPFMTNYCPEYESL
                     AYIPKVLTIHNGVYQGIFSWNNKDLLPYFRTYAAPILDWNGMINSLATGIKCAWRVTT
                     VSYSYMEELSNKSNGLESLIRSEWHKCRGILNGIDNETWNPATDPRIAYKLKKDVRSF
                     KSYNKLVISEKFRINPNLPLIVFIGRLVAEKGADLLPDLIFRCAQSGRKVSFVVLGTG
                     DRNLHQSFRHLQWHLQGMFDCSLEYNETLAHQLYAGADFILMPSRVEPCGLNQMYAMR
                     YGTVPIVRAVGGLIDTVWDHGDWEGRGFRFQQFTLDDALHAIDRAISVFHDQKEFARL
                     QAHDMALDFSWDSAAHNYLNLYKELT"
     gene            3298..4563
                     /locus_tag="PLG24_08525"
     CDS             3298..4563
                     /locus_tag="PLG24_08525"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HMZ24976.1"
                     /translation="MIKMISLILGGGAGTRLYPLTKHRSKPAVSIGGKYRLIDIPISN
                     CLNSGVKRMFVLTQYNSASLNKHIKNTFQFDKFSHGFVDILAAEQTPSSKQWFQGTAD
                     AVRKSMHHLVNHDYDYVLILSGDQLYQMDFEEMVMAHIEKNAELSIATIPVVARDATG
                     FGIMKVDDTSRITSFVEKPNAEDLPEWVSPMSESNQKAGKNYLASMGIYIFNRPTIEE
                     LFEQNPAADDFGKEIIPIAISEGRKVVSYQYEGYWTDIGNIRSFFEANLELTDDVPKF
                     NLFDNARMIYTRARMLPPSKIFGSKIQHSIISEGAILHAESIIRSVIGIRSRIGANTT
                     IKASIVMGNDFFETIEDIVSNKGRIPMGIGNNCYIENAIIDKNARIGSDVRIQGSTTK
                     ANVEEEGYCIVDGIVVVKKNAVIPSGTVI"
     gene            4621..>5616
                     /locus_tag="PLG24_08530"
     CDS             4621..>5616
                     /locus_tag="PLG24_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HMZ24977.1"
                     /translation="MATFLCIASYYKGNDFLIACKEAGNKVYLVTSKGLEDKPWARSH
                     IDNIFYIQEDQNDEWNLDDLIKGTAFLMRSEKIDRVVALDDFDVEKAAALRETFRLPG
                     MGQTTAHHFRDKLSMRMKAFEKGIKVPAFSGLFNDDEVNHYLSTVKAPWLIKPRGEAS
                     ATGIVKCHTREDAWTVIQSLGDKRYQYLIEQFRPGDVYHVDAINDKKKVVFAQVSKYV
                     NTPMEVSHGGGIFRSHTVEYDSYDDKELQKLNADIMKAFGMNYSATHTEFIKCHEDGH
                     FYFLETASRVGGAHLAEMVEFATGVGLWTEWAKVESHSASGTEYRIPKKEKFHAGI"
BASE COUNT         1683 a         1016 c         1195 g         1722 t
ORIGIN      
        1 gtttcagcgt gcctaaagcg caggcttaat cgaaaaggga aggttgaatc ggtccgggtt
       61 ttattccaag gtgatcatag gcttttcttg tgacaatcct accgcgtggt gtcctttgta
      121 tgaagcctga catgatgaga aaaggctcat atacttcttc gatggtgcca ctttcctctc
      181 ccactgctgt gctaatggtg ctgataccta caggtccgcc attgaatttt tcgatgatgc
      241 tacgcaaaat ccggttgtcc atttcatcca aaccgctttc atcgacattc aaggcattca
      301 ggcccattct ggcgatgtcg agtgtgatca caccctctcc tttgatctga gcaaaatcac
      361 ggactcttct tagtaaagca tacgcaattc tgggagttcc gcggcttctt cttgcgatct
      421 catggattcc gtttcgttcc gttcttacgt taagtattcc tgctgctctt ttgataattc
      481 cctccagggt aggtatgtcg taataatcca ggtggaaact tatcccaaac ctcgctctca
      541 tcggtgctgt caacaacccc tggcgtgtag tagccccgat tagtgtaaaa ggattaagtg
      601 ctaactgaat gcttcgggca ttaggtccgg aatcaatcat gatatcgatc ttataatcct
      661 ccatggcaga atatagatat tcttccacaa ctgtattcag tcggtgtatt tcgtcaataa
      721 acaggacatc attcggttca aggttggtta gtaaaccggc caggtctccg ggcttatcca
      781 ggactggtcc tgaagttact ttaagattgg tgcctagttc gttggcaata atattggaca
      841 aagtagtctt ccccaatccc ggaggtccat ggagtaaaac atgatcaagg gcctctcctc
      901 gcagtttagc tgctttgata aagacctcaa gattatctac aatcttcctt tgccctttga
      961 agtcaccaag ataaccgggt cgaagcgtct tttcgatgat tttatcttcc ttgctcagat
     1021 ggctggtatc ggaatttaat aattcgtttt gcacaatata tatgatagaa agcaaagata
     1081 tgattttttt aatattaaat ctgattggta tagttagtat atattacaaa ttgcacaaat
     1141 aataaataaa caagtgcttt catagactgt gtttttgcgt atgattggtt ttttgttata
     1201 cccggcaatg aatacaataa aagtcccaaa accaaaatag cgaatattag ggtgttaaaa
     1261 ggttctataa ttggcccatc catgcattta ccatagtaag tttttttatt gacagcaaag
     1321 aatctaaata aactaacgat ttataatgtc agataaatta ttagttaata tacagagccg
     1381 cagtttatta tactttttat gtttagtgaa ttgtcaaaca attatatagt ctttttttaa
     1441 aattataaat atattacgag attattatca gtttgttata attgatttac tatgtcccca
     1501 atgctgtttg aatctggttt ttcttctttc cattcaggaa tgagtaattt ttgattgaaa
     1561 tttaatccat aattgcctta tagtataatt caggttgaat caacaaatga tatgcattaa
     1621 aatgcggata ttaaagggac tttgtgatat tatagggtga atcctgcgct atgtaataaa
     1681 aaattatttt ccaattaaga ttttttagtt cctacaatat ctatatattt atttcaaaat
     1741 ttgaattgat ttcgcatcaa aaattttaaa caaactcgtt ttataataaa ttaatatata
     1801 attatcctct aatgaaagta ttacatgttg ctgccgaatg ttatcccgct gcaaaagccg
     1861 gaggattggg tgatgttgtc ggagcgttgc caaaatatct gaaccgtatc gggatgcaag
     1921 ccggagtcat cataccaaag tatggaacca aatggttgtg ggaacatcag tttaaaacta
     1981 tttttaccgg tactgttcga ttaaatagaa gctatatcag atatagcatt gagcaagaag
     2041 tcgatgatat ccttggcttt acgttatttg cagtccatgt acccggtatg tttgatcgcc
     2101 ccggtgtgta tgctgatcca acaaccggtt atggttacag tgacgagctg gagagatatc
     2161 tggtattcca acaggctgta ttacaatggc ttatatcaag tgctgagagg ccaaatgtca
     2221 tccattgtca tgatcatcat actgccctga ttccttttat gacaaattat tgcccggaat
     2281 atgaatcact tgcctatatc ccaaaagtct tgaccatcca taatggtgtg tatcagggaa
     2341 tcttcagctg gaacaataag gacctcctgc cctattttcg aacctatgct gctccgatac
     2401 ttgactggaa cggtatgatc aacagccttg ccactggaat taaatgcgcc tggcgagtca
     2461 ctaccgtctc ttattcatac atggaagaac tttcgaataa gagtaacgga ttagaatctt
     2521 tgataagatc ggaatggcat aaatgtagag gtattttgaa cggaattgac aatgagacat
     2581 ggaacccggc aacagatccc cgaattgcct acaaactcaa aaaagatgtc cgcagcttta
     2641 aatcgtataa taaattggtt atcagcgaaa aatttaggat taatcccaat cttcctttga
     2701 ttgtgttcat aggccgcctt gttgctgaga aaggtgcaga tctcttacct gacttgattt
     2761 tcagatgtgc ccagtcaggc agaaaagttt cctttgtagt attgggtaca ggcgacagaa
     2821 atcttcatca gagttttcgg catcttcaat ggcacttaca aggtatgttt gattgttcgt
     2881 tagaatataa cgaaaccctt gcgcatcagc tttatgccgg agcggatttt atcctgatgc
     2941 catcaagggt cgagccttgt ggtcttaatc agatgtatgc catgcgatat ggtacggtgc
     3001 cgatcgtccg cgccgttgga ggattaattg acacggtttg ggatcatgga gactgggaag
     3061 gaagaggatt tcggtttcaa caattcacgc ttgatgatgc tttacatgca attgacagag
     3121 caatttccgt atttcatgat caaaaagaat ttgccagatt gcaggcccat gatatggctt
     3181 tggattttag ttgggacagc gctgcacata attatttgaa tctttataag gaattaacat
     3241 aatttttaaa aattatatta atattacaag tcgaatgaat atataaaatg attgactatg
     3301 attaaaatga tcagccttat tttaggcggg ggagccggga cacgacttta tcctttaact
     3361 aagcatcgtt ccaagcctgc tgtatctatt ggtggtaaat accgtttgat tgatatacct
     3421 atttctaatt gtctgaattc aggggttaag agaatgtttg tgttgaccca atataattca
     3481 gcatcgctta acaaacacat aaagaataca tttcagtttg acaaattctc tcatggattt
     3541 gtagatattc ttgctgccga acaaacgcca agtagtaagc agtggtttca aggtacggct
     3601 gatgcggtcc ggaaatcaat gcaccacctg gtcaatcacg attatgatta tgtcttaatt
     3661 ctgtcaggtg atcaattgta tcaaatggat tttgaggaaa tggtcatggc tcatatagag
     3721 aagaatgcag aattgtcaat tgcgactatt cctgttgtag ctcgtgatgc cacagggttt
     3781 ggcattatga aagtcgatga tacttcccga ataaccagtt ttgtagaaaa gccaaatgca
     3841 gaagatttac ctgaatgggt atctcccatg tctgaatcca atcaaaaagc aggtaaaaat
     3901 tatcttgcat cgatgggcat ttatattttc aataggccta caatcgaaga gctatttgag
     3961 caaaatcctg ctgcagatga ttttgggaaa gaaatcattc ccatcgcaat atctgaaggt
     4021 agaaaggtag tcagttatca gtatgaaggc tactggactg atattggaaa tattcgatcc
     4081 ttctttgaag ccaatttgga attgacggat gatgttccaa aattcaatct ttttgacaac
     4141 gcaagaatga tttataccag ggcaaggatg cttcctccct caaaaatttt cggaagcaag
     4201 attcaacaca gcataatatc agaaggtgca attctgcatg cagaatccat tatccgttcg
     4261 gtcatcggta ttcgttcaag aattggagcc aatacaacta taaaggcttc aatcgtcatg
     4321 ggaaatgatt tttttgaaac gattgaggat atagtgtcta ataaaggtag gatcccaatg
     4381 ggaatcggta ataactgtta tatagaaaat gccatcatcg ataaaaatgc aagaattggc
     4441 tcagatgtcc gaattcaggg gagcacaacg aaggcaaatg tagaagagga aggatattgt
     4501 atcgtcgatg gcatagtggt tgtaaagaaa aatgctgtca taccgtccgg aacagtgata
     4561 taaaatattg attaatcaga gcctgaataa attataaata agaaaattaa tgtaagcaat
     4621 atggctactt ttttgtgtat cgcgagctat tataagggaa atgatttttt gatagcctgt
     4681 aaggaagccg gtaataaagt atatttggtt acttccaagg gattagaaga taagccttgg
     4741 gcgaggtccc atatagataa cattttttac atccaggaag atcaaaacga tgagtggaat
     4801 ttggatgatt tgatcaaagg gactgctttt ctgatgcgtt ctgaaaaaat tgaccgggta
     4861 gttgcgttgg atgactttga tgtggaaaaa gctgctgccc tacgcgaaac tttcagattg
     4921 ccgggaatgg gtcagactac tgcacatcac tttcgcgata aattatccat gaggatgaag
     4981 gcgtttgaaa aaggcatcaa ggtgccggca ttttcaggct tgttcaatga tgacgaagta
     5041 aatcattatc tttcaactgt taaagcgccc tggttgataa aacctcgggg tgaggcttct
     5101 gcgaccggta tcgtaaaatg ccataccagg gaagacgcat ggacggttat tcagagtctg
     5161 ggggacaaaa gatatcaata tctgattgaa caattcaggc ccggcgatgt ctaccatgtg
     5221 gatgccatca acgataaaaa gaaggttgta tttgctcagg tgagcaagta tgtcaatact
     5281 ccgatggaag tatctcatgg tggggggata ttccgctcac ataccgtaga gtatgatagt
     5341 tacgatgaca aagaacttca aaagctcaat gcagatatta tgaaggcctt tggtatgaat
     5401 tatagtgcaa cacataccga gtttatcaaa tgtcatgaag atgggcattt ttattttctt
     5461 gaaactgcct cacgagttgg cggtgcccat ctggcggaaa tggtcgagtt tgctaccgga
     5521 gtaggccttt ggacggaatg ggccaaggtt gagtcgcatt cagcatccgg aacagagtat
     5581 aggataccta aaaaagaaaa gtttcacgca ggtatt
//
LOCUS       DAOQQN010000211         5598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6402_length_5598_cov_5.099224, whole genome shotgun sequence.
ACCESSION   DAOQQN010000211 DAOQQN010000000
VERSION     DAOQQN010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6402_length_5598_cov_5.099224"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PLG24_08535"
     CDS             complement(<1..680)
                     /locus_tag="PLG24_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HMZ24978.1"
                     /translation="MIPEKLTIEGLYSYREKQEIDFARLTEAGLFGIFGMVGSGKSSI
                     LEAITFALYESIERLGYNDTRYYNMMNLRSNRMYIDFEFRNYEDRQFRITKEIKRNSR
                     NFLDIRTPVSSFYEKISGSWTPIESPNIKDIIGLSYDNFKRTIIIPQGRFREFLELGH
                     KDRTAMVMEIFDLKRFDLYDKGKKIEAANKSRLDQLQGQLGTYEDVTVDALDVLKESI
                     TRAEGELQR"
     gene            complement(686..1891)
                     /locus_tag="PLG24_08540"
     CDS             complement(686..1891)
                     /locus_tag="PLG24_08540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HMZ24979.1"
                     /translation="MKILHTADWHLGKKLDSFSRIDEQMEVLAEICEIADTERPDMVI
                     VAGDLFDAFNPSTEAIELLYRTLKRLTANGTRPVIAISGNHDSPALIDAPEPLARECG
                     IIMAGYPDAEIKPLELPGFSIIKSAPGFIELKIGHLNHPVRVILTPYANEVRMKEYFG
                     DEKDKMLNQSLHDRWHNLADHYCDDTGVNILTAHLYMMNRQGAILEEPEGEKPIRIGN
                     ADLVYSESIPSLIQYTALGHLHGFRNVGTEDRPAIYSSSPLPYSFSEAGQDKYVVIID
                     VEPGKNALVRKSVLQKGRRLYRKTFDDIDLAVQWLDNHPNALIELTIESDSYITASDR
                     KRLHQAHDGIIHLIPKIRNNDVEGVETSEINISQDIRQLFSDYFKTKNAGQEPNEEIM
                     NLFNEILQQ"
     gene            complement(1983..2849)
                     /locus_tag="PLG24_08545"
     CDS             complement(1983..2849)
                     /locus_tag="PLG24_08545"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HMZ24980.1"
                     /translation="MEGHHINNHGLIACCVNSRAGNGKAVKLAGRVSDILKEKAIAHQ
                     VLMEPWPDQLDGFSEAWIFGGDGTVNYFVNQYPEIDIPLAVFAGGTGNDFAWKLSGEV
                     QLTQHVESVLTVQPRRIDAIRCNERLALNGIGLGFDGEVLKFIDSIRFIGGHLGYQIA
                     VLFKIFTFREIEFTVYCDGIRTDDKFLLLMICNSSRMGGGFMVAPMAEISDGWLDLIM
                     VKPMGVMQRLFNLPKLEKGLHINTEYTTTKKIRGITITPSTRCPIQIDGELFYYDQLD
                     IEILPKKFRFKY"
     gene            complement(2865..4331)
                     /locus_tag="PLG24_08550"
     CDS             complement(2865..4331)
                     /locus_tag="PLG24_08550"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HMZ24981.1"
                     /translation="MKKLLYFLAIFTVTTSLPVCAQDFLPIFQRINDDVLKNGRAYEN
                     LREATTSIGHRLTGSDNGSKAEDYAFNLLKSYGYDNVQYQEFNVEAWTRGTVSLIIGT
                     GNNAKSIPVVALAHSPVSAHVSGKIIDLGDGLEEDFIKAGDGVRDKLVLVNINIQSPD
                     NKGKKNLHRSEKTALAIKYKAKGIIIANGVKGGVLLTGTASVTGQLIPIPAVCISLES
                     GQEIRKFLSEDSALDARIDMTNASRPVRARNIIATLPGTSPETGQEIIIIGGHLDSWD
                     LATGAVDNGIGSFSILDMARVFKQLRLKTARTIQFVLFMGEEQGLLGSKHMVSELNKS
                     GEIKDVRLMINLDMNNNTRGFNAGGNTTLLPTLEQIGTVIKQVDTSYANDIKNSTGLH
                     SDHQPFMLSGIPTATSVGSLPPGAWGCYHADCDHFSLIIKDQLDNNVRYVSMVLYALA
                     NISHLPVAAMSDTETRDFLIKNNLKAELILGKEWRWEE"
     gene            complement(4652..>5598)
                     /locus_tag="PLG24_08555"
     CDS             complement(4652..>5598)
                     /locus_tag="PLG24_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HMZ24982.1"
                     /translation="ENIKRALEIAAAGGHNVILIGPPGAGKTMLAKRLPTILPQLSLK
                     EALETTKIHSVAGILPRDASLITQRPFRSPHHTISDVALVGGGSNPRPGEISLAHNGV
                     LFLDELPEFKREALEVMRQPMEDRVVTISRARQTIDYPASLMLVASMNPCPCGYYNHP
                     EKDCVCAPGVVKKYLNKISGPLMDRIDIQVEVTPVSYDKLAEAGPSLERSDDIRARVE
                     SARKIQEERFHDVKNVYSNAQMPGKMVRDVCRLPEAGLALLKNAMIKLQLSARAYDRI
                     LKVSRTIADLEGCEDIRTEHLAEAIHFRNLDKESWAPA"
BASE COUNT         1356 a         1309 c         1313 g         1620 t
ORIGIN      
        1 cgctgcagtt cgccttcggc ccgggtgatg ctctctttca acacgtcgag agcatcgaca
       61 gtcacatctt cgtacgtacc gagttgacct tgtaattgat cgaggcggga tttgttggcc
      121 gcctcaattt tttttccttt gtcgtatagg tcaaatcgct tcagatcaaa tatttccatt
      181 accatggcag tacgatcctt gtgccctaat tcaagaaact ctctgaacct gccctgaggt
      241 atgatgatgg tacgtttgaa gttgtcataa ctcaggccta tgatgtcttt gatatttggt
      301 gattcaattg gcgtccagct gcctgatatt ttttcataaa aacttgacac cggtgtcctg
      361 atatccaaga aattgcgtga attgcgctta atttcttttg tgatacgaaa ctgacgatcc
      421 tcataatttc ggaattcaaa atcaatgtac atcctgttgg accgtagatt catcatgttg
      481 taatatcggg tatcgttata tccgagccgt tcaatgcttt cataaagcgc aaaggttatt
      541 gcttccagga ttgaagattt gcctgagcca accattccga atatgccgaa caagccggct
      601 tctgtaagtc gtgcaaagtc tatttcctgt ttttcccgat aggaatacaa gccctcgatg
      661 gtcagttttt cagggatcat agggcttatt gttgcaggat ttcgttaaac aagttcataa
      721 tttcttcatt tggttcctga ccggcatttt tagtcttgaa atagtcgctg aacaattggc
      781 ggatgtcttg actgatattg atttcagaag tttcaacgcc ttcaacgtca ttgttccgga
      841 tctttgggat aagatgtatg attccatcgt gtgcctggtg tagccgcttt cggtcggaag
      901 ctgtgatgta ggaatcgctt tctatggtca gttcgatcag ggcattaggg tgattgtcca
      961 gccattgcac cgccagatca atgtcgtcaa atgttttccg gtaaagcctc cggccttttt
     1021 gcaagacact tttccggaca agggcgtttt tcccgggttc tacgtcgatg ataacaacgt
     1081 acttgtcctg acccgcttcc gaaaagctat atggcaaggg agaagaggaa taaatagccg
     1141 ggcggtcttc ggtgccgaca ttgcgaaaac cgtgcaaatg acccagggct gtatattgga
     1201 tcaggcttgg aatggattca gaatatacaa ggtcggcatt gccgatgcgt atgggttttt
     1261 caccttcagg ctcttcgagg atggcgccct gacggttcat catgtaaagg tgtgccgtca
     1321 gaatattgac tccggtgtca tcgcaatagt gatccgcgag gttatgccac cggtcgtgca
     1381 gactttgatt aagcattttg tccttttcat cgccgaagta ttccttcata cgcacttcgt
     1441 tggcataagg tgtgaggatg acgcgtacgg gatgattcag gtggccaatc ttcaattcaa
     1501 tgaaccccgg ggcggactta atgatggaga atcccggtaa ttccaagggt ttgatctccg
     1561 cgtccggata accggccata ataatgccac actctcgtgc caagggctca ggagcgtcga
     1621 tcagagcagg tgaatcgtga ttgccactga tagcgatgac cggtctggta ccgttagccg
     1681 tcaatcgttt gagcgtgcgg tacaatagct cgattgcctc agtggatgga ttgaatgcat
     1741 cgaaaagatc acccgcgacg atgaccatat ccggcctctc cgtgtcggct atctcacaga
     1801 tttcggccag tacttccatt tgttcgtcga tacgcgaaaa ggaatccagt tttttcccaa
     1861 ggtgccagtc ggcggtatgt aatattttca tttaagctaa tatgagaaaa attcgcaaaa
     1921 agagagaatt tgttttttgt caaatgaatt tcgcagttct gaagaacaaa atcttcatcg
     1981 gttcaatatt taaaccggaa ctttttcggc aaaatttcga tgtccagctg gtcgtaataa
     2041 aacaattcgc catcaatctg gatgggacag cgggttgaag gcgtgatggt tattccgcga
     2101 attttttttg tcgtggtgta ttctgtattt atgtgaagac ccttttccag ctttggtaaa
     2161 ttaaaaagtc tttgcatcac gcccatcggt tttaccataa tcagatcgag ccatccatcg
     2221 ctgatttcag ccatcggggc gaccatgaag ccaccgccca tgcgcgatga attgcagatc
     2281 atcaacagca ggaatttgtc atcagtcctg ataccatcac agtaaacggt aaattcaatt
     2341 tcccggaaag taaatatctt aaatagaaca gcgatttgat atccgagatg gccaccgatg
     2401 aatcgtattg aatcgatgaa tttcaacacc tcgccatcga atcctaatcc gataccattg
     2461 agggccagcc gttcattaca ccggatggca tcgattcgtc ggggttgtac agtaagtacg
     2521 ctttcgacgt gttgggtcaa ttggacttcg cctgacaatt tccacgcaaa atcatttccc
     2581 gtgccgcctg caaatacagc cagggggata tcgatctctg gatattgatt gacaaaataa
     2641 ttgactgtgc catccccgcc aaatatccag gcctcggaaa atccgtcgag ttggtccggc
     2701 cagggctcca tcagcacttg gtgtgcaatg gccttttcct tcaggatatc gctgaccctc
     2761 cctgccagct taaccgcctt gccattgccg gcccgggagt tgacacagca agcgattagt
     2821 ccatggttgt ttatgtgatg tccttccatt ctctttgatg tggcttattc ttcccagcgc
     2881 cattccttgc ccaggatcaa ctccgccttc aaattgtttt ttatcaggaa atccctggtt
     2941 tctgtatctg acatggcggc aacaggaaga tgggaaatat tggccagcgc gtacaacacc
     3001 atgctgacat atcggacgtt gttgtccagt tgatccttta tgatgaggct gaaatgatca
     3061 caatctgcgt gataacaacc ccaggcaccc ggaggaagtg agccgacgga tgtggcggtt
     3121 ggtattccgg agagcatgaa gggttgatga tcgctgtgca acccggtgga attctttata
     3181 tcattggcat aggaagtgtc tacctgcttt attaccgtac caatttgttc aagtgtcggc
     3241 agcagggtgg tgttgccacc tgcattgaaa ccccgggtgt tgttattcat gtcgagattg
     3301 atcatgagtc ttacatcctt gatttcgccg gacttattca gttcgctaac catgtgtttt
     3361 gagcccagca gaccttgttc ttcacccatg aaaaggacaa attggatggt acgggctgtt
     3421 ttaagtctga gttgtttgaa cacccttgcc atgtcaagga tagaaaagga gccaatgcca
     3481 ttgtcgacag cacctgtggc aagatcccag gagtccaggt gtccacctat gatgatgatc
     3541 tcctgacctg tttccggaga ggtccccgga agagtggcta tgatattgcg tgccctgaca
     3601 ggccggcttg cattggtcat gtcgattcgg gcatcaagcg cagaatcttc agagagaaat
     3661 ttcctgatct cctgacctga ttccagggaa atacatacag ccgggatagg tatgagctga
     3721 ccggttacgg atgctgttcc ggtgagcagg actccgccct tgacaccatt ggcgatgatg
     3781 atgcctttcg ctttgtattt gattgccagg gctgtctttt ccgagcggtg caggttcttt
     3841 ttacccttgt tgtctgggga ttgaatattg atattgacca ataccagttt gtcccttacc
     3901 ccatcgcctg ctttgataaa atcttcttcc aggccatcac ccaggtctat tattttccct
     3961 gaaacatgtg ctgacacagg tgaatgggcc agagctacaa ccggaatcga cttggcatta
     4021 tttcctgtcc cgattattaa ggagacggta cccctcgtcc aagcctcaac attgaattcc
     4081 tgatattgaa cattatcata gccataagat tttaacagat tgaaggcgta gtcttcggct
     4141 ttgcttccgt tatcacttcc ggtcaaccta tgaccgatag aagttgttgc ttcccgtagg
     4201 ttttcatagg cacgtccatt tttcagcaca tcgtcattga ttctctgaaa tatcggcaga
     4261 aaatcctgag cacataccgg aagagaggta gtgaccgtaa aaatcgcgag gaaatacaat
     4321 agttttttca tagtaaaaaa taaatactcc aatacaattt gacaaaaaga aataaatgta
     4381 gtaaaagtct tgcaattatt tcactacacc caaaactact gttaatggtc atgctttttg
     4441 acttctacag gtaaatttat tttatcattg cttgccgggt gttttaatgg tcaatataga
     4501 tatcgccctt cagaaccagg cgtcatatgc ttgttttaaa ttttattcat ccaaactatc
     4561 ctgatgaata aatatactac tctgatggat caatccaaat ctggggaaga tccgtatatt
     4621 atagatatgg gcaacaagat gtcaattctg tttatgccgg tgcccaagat tccttatcca
     4681 gattcctgaa gtggatcgcc tctgccaggt gttccgtcct gatatcctcg caaccctcca
     4741 ggtctgcaat ggttctgctg actttcagga ttctgtcgta ggcacgggcg gacagttgca
     4801 acttgatcat ggcatttttg agcaatgcca ggccggcctc gggcaatcgg catacatctc
     4861 tgaccatttt accgggcatc tgcgcattgg aatatacatt cttaacatca tgaaagcgct
     4921 cttcctgaat ctttctggca gactcgactc tggcgcggat gtcatcactt ctttccaggg
     4981 aaggcccggc ctcggctagt ttgtcgtaag atacaggtgt tacctcaacc tgaatatcga
     5041 ttctatccat caagggtcct gagatcttgt tcaggtattt cttgactacg ccgggtgcac
     5101 atacacaatc cttttcagga tgattgtaat agccgcacgg acagggattc attgaggcaa
     5161 cgagcatcaa gctggcggga tagtcgatgg tctgtctggc cctactgatg gttactaccc
     5221 tgtcctccat gggctggcgc atcacttcca gtgcctcccg cttaaattca ggtaattcgt
     5281 ccaaaaaaag gacaccgttg tgcgccaatg atatttctcc cggcctcgga tttgatccac
     5341 ctcctaccag agccacatcg ctgatggtat ggtgcggaga acggaaaggc cgctgagtaa
     5401 ttaatgaggc gtcgcgtggc agaataccgg ctactgaatg gatcttggta gtttccagcg
     5461 cttcctttag cgatagttga ggcaggatgg tcggcagcct cttggccagc atggtcttgc
     5521 ccgcacccgg cgggcctata aggatgacat tgtggccgcc tgcagccgct atttccaggg
     5581 ctcgcttgat attctcct
//
LOCUS       DAOQQN010000212         5557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6482_length_5557_cov_5.647946, whole genome shotgun sequence.
ACCESSION   DAOQQN010000212 DAOQQN010000000
VERSION     DAOQQN010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6482_length_5557_cov_5.647946"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PLG24_08560"
     CDS             complement(<1..372)
                     /locus_tag="PLG24_08560"
                     /inference="COORDINATES: protein motif:HMM:NF025118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HMZ24983.1"
                     /translation="MTTDIKSYTLLELNTYIKRVLALNFDNELWVRAEILNAAEKRGH
                     YYLTLIQKSSVSNDIVAQLEAVVWASDFQRIKKENPDLVNFFTSGFEIMSHGQPVFHE
                     RFGLQFRISDINTEYARGALDR"
     gene            complement(369..1304)
                     /locus_tag="PLG24_08565"
     CDS             complement(369..1304)
                     /locus_tag="PLG24_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24984.1"
                     /translation="MFISSISYTASSFSEDKGFFKISGPDYSGHFEKNEIRRLSPIII
                     TAVGCGLETNTNTNRELCGIITGTGRGCLSKTEQFLRDITTYKETALNPTHFIQSTNN
                     TIGGMIALKLNVNTYNMTYVNQGLTVLNVLTDTEILVKENPGKNVLFGFFEENTEFNE
                     KIHRDSGFLFEKDDNKIFYGEGTSFFLISDQKDNAIAMISEYTLFVNSEKKISDDIHT
                     YLTNIPWDSNSAIYAGYNNEFEKHHFYKDISSFATGRKIPEITFKHQTGDFDTASGHA
                     MAMAVDDITKGKYNRIVIFNHYKKKSLHSIIISKP"
     gene            complement(1304..2518)
                     /locus_tag="PLG24_08570"
     CDS             complement(1304..2518)
                     /locus_tag="PLG24_08570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HMZ24985.1"
                     /translation="MGQNNAEVWITGIGILSALGNGFEENWAALKSGKSALRPIELSG
                     SIHDGKFFAGMVSLSDEELVKQLSELNIRYPHNRPTLMSLYTARQALRNADIEMFTDK
                     IVLISVTTVGGISSTEKHFDNLSLNKTKATQEFLSEMDCGSISRNVCSYLGLSKPHYM
                     ISTACSSSNNAIMLGARLIKAGKAEVVLAGGSDSLSKFVLNGFHSLKNLDPKPCTPFD
                     KDRFGLNLGEGAAYLVLESSKHATNRGKSSLAVVAGYGNISETYHITGSSPHGMGAYG
                     AMSLALTSANLNPQEIDFIHSHGTSTIDNDLAESNAIKRLFGNDIAFGSSKGYTGHTL
                     AAAGAISSVLSIGMMQHGFIFPTINFRHPIPETGLSPVTTIKENQHLRHIMVNSFGFG
                     GNNTSLIFSKAA"
     gene            complement(2520..2789)
                     /locus_tag="PLG24_08575"
     CDS             complement(2520..2789)
                     /locus_tag="PLG24_08575"
                     /inference="COORDINATES: protein motif:HMM:NF006617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HMZ24986.1"
                     /translation="MQNSMEQLIDKLKSEIIEALNLQDMSPDDIDTDAPLFGTGLGLD
                     SIDSLELMVLLERNYGIKIEDPREGRKVLESVRSMAQYIESFSNT"
     gene            complement(2957..5419)
                     /locus_tag="PLG24_08580"
     CDS             complement(2957..5419)
                     /locus_tag="PLG24_08580"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24987.1"
                     /translation="MLRDKANTPVGVLGVNWDITVLKEAERKLQDALEKENAVAQAQL
                     TALQSDEGKFKEQEASLNQQKYTFNKQKSELTDQLASLESHADELQEEIKVRNEELKL
                     LGEKREETAEKKKQVERKIDRVSHEIRLLKSLIDNQEGYPEAVRFLSTQQKNTETLLL
                     ADLINCEEKYRTPLENFLNPYLTHLVVNDYEDAFRMTDALSAGQKGKAGFIILEAFKE
                     KPIDSPEIEGALSLTSIVQCHPKFNNLFRHLLKGAYIVTDIKRQHLEAMNADHSLHFI
                     SEDGSMVLSHGHASGGSIGLIEGSLLGRKLRLESLQEELIELKKSDSEFYVELANIDD
                     KIGTISDEGKGPYLDKILKEVQQVKDALLQISVKAEYNQLQLDKISSDDVLLKKKVND
                     FSELIQRNLPSIASLKIEQESLLTSVQGKKETIRERSEELSSTGSLLNAAKFNALQVE
                     NHKTSLLREIDLFTQQVSGLVEQEKQILNIQSSGVVEMATLETQIQECENGIVEVAEK
                     IKASQSELTEAERVYFTEKKQVNEDEQAIKLMQRSLAGIHTIANEWKDKWHAAKFEMQ
                     RIEDNFSIEFGSTLPDEATTDESPDDTVLKELENQNQKIRHSIHQFGEVNPLAVEAYN
                     EIKARYDEIVSQKNDILDSKDKLLQALNELENTSTTKYLAAYEQVRENFKMVFRSLFT
                     EDDDCDLVIENPGTPLDSDIGIIARPKGKRPQSLSQLSGGEKTLTAIAFLFSLYLLKP
                     APFCIFDELDAPLDDVNVEKLNKIIRKFSKESQFIIITHNKATMAEVDVMYGVYMEQQ
                     GISGLSMVDFRTFEHNLVLEKV"
BASE COUNT         1418 a         1186 c         1091 g         1862 t
ORIGIN      
        1 ccgatccaat gcccctcttg catactctgt attgatatca gaaatcctga attgcaggcc
       61 gaatctttcg tgaaataccg gttgcccatg gctcatgatt tcaaatcctg acgtaaagaa
      121 attgaccaga tcaggattct cttttttgat tctttggaaa tccgatgccc ataccactgc
      181 ctctaattga gctacgatat cattggagac ggaacttttc tggatcaaag tcaggtagta
      241 gtgacctctt ttttcagccg catttaatat ttcagctctt acccacagct cattgtcgaa
      301 attcaaagca aggaccctct tgatgtaggt attcaactcc aataatgtat aggatttgat
      361 atcagtagtc atggctttga tattataatc gaatgcagtg atttcttttt ataatgatta
      421 aagataacga ttctgttgta tttcccctta gttatgtcat cgactgccat tgccattgcg
      481 tggccggaag ccgtatcaaa atctccggtt tggtgcttaa atgtaatttc cggtatcttt
      541 cttccggtag caaaagatga aatatcttta tagaaatgat gtttttcgaa ctcattgtta
      601 taaccggcat aaattgctga attgctatcc catggaatgt tggtcaggta agtgtgtata
      661 tcatccgata ttttcttttc cgaattaaca aatagagtat attcggagat cattgcaatg
      721 gcattgtctt tttggtcgga gataaggaaa aaactggtgc cttcgccata aaagattttg
      781 ttgtcatctt tttcgaaaag gaaacctgaa tctctgtgta ttttttcatt aaactcggtg
      841 ttctcctcga aaaatccaaa caagacattt ttgcccgggt tttccttgac cagtatttcg
      901 gtgtcagtca ggacatttag cacagtcagc ccttggttga cataggtcat attgtatgta
      961 ttgacattca atttaagagc gatcataccc ccaatggtat tattggtgga ttgaataaag
     1021 tgggtaggat tcagcgctgt ttctttatag gtcgtgatat cccttagaaa ttgttctgtt
     1081 ttggataggc agcctctacc tgttcctgtg atgattccgc ataattctct gttagtattg
     1141 gtgtttgtct caaggccaca accgacagca gttattatga taggactgag cctccgtatt
     1201 tcgttttttt caaaatgtcc tgaataatcc ggccctgaga tcttaaaaaa gcctttatct
     1261 tccgagaaag atgatgctgt ataggatatg ctggaaataa acatcatgcc gcttttgaga
     1321 atatcaggga cgtattattg cccccaaaac caaatgaatt taccattatg tggcgtaaat
     1381 gttgattttc tttgatagtg gttacagggg aaagtcctgt ttcaggaatt gggtgccgga
     1441 aatttatagt tggaaatatg aatccatgtt gcatcatacc aatggaaagg actgacgaaa
     1501 tggctccggc tgcggcaagt gtgtggcctg tatacccttt gcttgatccg aatgcaatgt
     1561 cattgccaaa aagtctcttt atggcgttgg actctgccaa atcgttatca attgtacttg
     1621 tcccgtgaga atggataaag tcaatttctt gaggatttag gttggctgag gtgagggcta
     1681 aactcatcgc gccataagct cccatcccat gtggtgacga gccggtgatg tgataagttt
     1741 cactgatatt accatagccg gcaacaacgg ccagggaaga ctttcccctg tttgtcgcat
     1801 gtttgctgct ttccagtacc aggtatgccg ctccttcacc cagattcagt ccaaatcgat
     1861 ctttatcaaa tggagtgcag ggtttgggat ccagattttt taatgaatgg aaaccattga
     1921 gtacgaactt actcaatgaa tcgcttcctc cggccagtac tacttcggct ttaccggctt
     1981 taatcaacct ggcaccaagc atgatggcgt tattagagga agaacaagca gtgctgatca
     2041 tataatgtgg cttgctcagg ccaagataac tacacacatt acgactaata ctgccgcaat
     2101 ccatttcact gagaaactcc tgtgttgcct tggttttatt cagactcagg ttgtcgaaat
     2161 gtttttcggt tgaagaaatg cctcctactg tagttacgct gatgagtaca atcttatcgg
     2221 taaacatttc aatatcagca ttccttaatg cctgtcgggc agtataaagc gacatcaatg
     2281 taggcctgtt gtggggataa cggatattta gttcggagag ttgtttgaca agttcttcat
     2341 cactcaaaga aaccattccg gcaaaaaatt tgccatcgtg aattgagcct gacaattcga
     2401 tcggtctgag tgcgcttttt cctgatttga gtgcagccca gttttcttca aagccatttc
     2461 caagggctga taagatgcca atgccggtaa tccagacttc agcgttattt tgtcccattt
     2521 taagtattac taaacgactc aatatattgg gccatggatc gaacactctc cagtactttc
     2581 cggccttctc tgggatcttc tatttttatt ccgtaatttc tttccagcaa aaccattaac
     2641 tccaaagaat caatactgtc gagacctagc cctgttccaa acaatggggc atccgtgtcg
     2701 atatcatctg gactcatatc ctgcaggttc aatgcttcaa taatttcgct tttcaatttg
     2761 tcgatcaatt gttccatact attttgcaga gaatttttta ataattggta ttttattttt
     2821 gctgttcgat cgctgattgt cttgataatt cactatgcta atccaatcgc gtgaattccc
     2881 ggagaataag cactctgtct gttccattat tagatacaaa catattaatt tttttctaaa
     2941 aatgtttaca ttttcactaa accttttcca acacaagatt atgttcgaaa gtcctgaaat
     3001 ccaccatact taacccactg atgccttgtt gttccatgta aacaccatac atgacatcaa
     3061 cttctgccat ggtagccttg ttatgtgtga tgatgataaa ttgcgattcc ttgctgaatt
     3121 ttctgattat cttgtttaat ttttccacat tgacatcatc aagtggagca tccaattcgt
     3181 cgaaaataca aaaaggagcc ggttttagca aatacaatga gaaaagaaaa gctatggcag
     3241 tgagcgtttt ttctccccct gacaactggc tgagactttg cggccttttg ccctttggcc
     3301 tggctatgat gccgatatct gaatccaagg gtgttccggg attttctatg accagatcgc
     3361 aatcatcatc ttctgtgaaa agactcctga acaccatttt aaaattttct cttacttgtt
     3421 cataggcggc aagatacttc gtagttgaag tattttccag ttcattgagg gcctgtagta
     3481 atttgtcttt tgaatctaat atgtcatttt tttgacttac gatttcatca tatctggcct
     3541 tgatctcatt gtatgcttct acggcaagag gatttacttc tccgaactga tggatggaat
     3601 gtcggatttt ttgattttgg ttttccagtt cctttaatac tgtatcatca ggactttcat
     3661 ctgtagttgc ttcgtcgggc aaggttgaac cgaactcaat ggagaaatta tcttcgatcc
     3721 gttgcatttc gaacttcgct gcatgccatt tatctttcca ttcattggcg attgtgtgta
     3781 tgcctgcgag tgacctctgc atcaatttga tagcctgttc gtcctcgttt acctgcttct
     3841 tctcagtaaa atacaccctc tcagcctcag tcagttccga ttgagacgct tttatttttt
     3901 cagccacttc aactatgcca ttttcgcatt cttgaatttg agtttccaat gttgccattt
     3961 caacaacacc tgaactttgg atattgagga tttgtttttc ctgttcgacc aacccggaaa
     4021 cttgctgggt gaataaatca atttcccgaa gcaatgaagt tttatgattt tcaacttgaa
     4081 gggcattaaa cttagctgcg ttcaacaaac ttcctgtact actcaattct tctgatcgtt
     4141 ctctgattgt ttcctttttg ccctggactg aagtaagaag ggattcttgc tctattttca
     4201 acgaagcgat agacggaaga ttcctttgaa tcaactcgct gaagtcattg accttttttt
     4261 tcaacaatac gtcgtctgac gatattttat caagctgaag ctggttatat tctgctttta
     4321 ccgagatctg cagcaatgca tctttgacct gttgaacttc tttgagtatc ttgtcaagat
     4381 aaggcccctt tccttcatca gagatggttc ctatcttgtc gtcgatgttg gccaattcca
     4441 cataaaattc agaatcagat ttcttcaact ctatcaactc ctcttgcaat gattccagtc
     4501 tcaacttacg tcccagtaga ctgccttcga tcagaccgat ggatccacct gatgcatgac
     4561 catgagaaag aaccatgctt ccgtcttcag aaatgaagtg taagctatgg tcagcattca
     4621 ttgcttcaag atgctgcctc ttaatatcag tgacgatgta agcgcccttg agtaaatgcc
     4681 tgaacagatt attgaatttg ggatgacatt gcacaatgga tgtaagtgac aatgctccct
     4741 caatttcagg cgagtcgatc ggtttttcct tgaatgcttc taatatgata aatccggcct
     4801 tacccttttg acctgcagaa agagcatcgg tcattcgaaa tgcatcctca taatcattga
     4861 ccaccaagtg cgtgagatat ggatttaaaa agttttccag aggagtccgg tatttttcct
     4921 cacagttgat aagatcagcc aaaaggagtg tttctgtatt tttttgttgg gtgctaagga
     4981 aacgtactgc ttcgggatat ccttcctgat tatcaatcag ggactttaat agtcttatct
     5041 catgacttac ccggtctatt ttacgttcaa cctgcttttt cttttcggcg gtttcctccc
     5101 tcttttcacc aagcagtttc aattcttcat ttctaacttt gatctcctct tgcaactcat
     5161 cggcatgtga ctccaacgaa gcaagttgat ctgtcaattc ggacttttgt ttgttaaagg
     5221 tatatttttg ttgatttaag gaagcctctt gttccttgaa cttcccttcg tcactttgga
     5281 gtgctgtaag ctgagcctgt gcaacggcat tttccttttc aagggcatcc tgcaattttc
     5341 gctcagcctc tttcaagacc gtaatatccc agttcacacc aagcacaccc accggcgtgt
     5401 ttgctttatc gcgcagcacg atggctctta cctttagata atgggtcgag ctgtcttgcc
     5461 agattatgcg aaattctgtg tcgagtggat tctctccgtg tatggcagca tcgcgtactc
     5521 ccagaagatt atgtctgagg gtcgggttta ttcaggc
//
LOCUS       DAOQQN010000213         5546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6505_length_5546_cov_6.189765, whole genome shotgun sequence.
ACCESSION   DAOQQN010000213 DAOQQN010000000
VERSION     DAOQQN010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6505_length_5546_cov_6.189765"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PLG24_08585"
     CDS             <1..391
                     /locus_tag="PLG24_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HMZ24988.1"
                     /translation="GALSWRPGEDMVAGFSNYGAKNVDLFAPGVDLYSTVPNQSYKKE
                     SGTSMAAPVVAGVAVLLRSYFPTLSAKQTKEIILQSVHKVNAMVKKPGTDELVPFSSL
                     SVTGGYVDAYEAIKLAAKKTGVKLAMP"
     gene            complement(496..2697)
                     /locus_tag="PLG24_08590"
     CDS             complement(496..2697)
                     /locus_tag="PLG24_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ24989.1"
                     /translation="MEMDHLWRDRFWWEAVLKYSGSIAGCDLFGSLKFYRQEPPKFIV
                     KDTAFCGSGTYRIPVEIVNHAGELYYSFEGQGFHMPVDIGLNYIDLDFNASGGVYIDS
                     LKTQYRTCNDLQSDTLDVRIENMPESLLPDTITLCNTYNRDGKPVYFLPDLIVSGERS
                     GNWNLSSLSGITISNDSIDMSHVAVGTYKVNFRTNTAVLPCKDVTYATVFKVVDCSCN
                     RPALNKLKDTYRICSNYGSLNLDSLAGVNVPVQWFDLTSGSKEAIGGSNLLINDQFVG
                     VHSILLQTVADWEGACADTILFELQVVPRFYAGDDYLMDICNGEDYLIDLDTLLINSL
                     NDGAWKAQKPDYPGFSIAFDPQTNTISTKKLFPGDYMLDKIVAGSDVCPSDTASLTIK
                     VYPLPEILVSGTNYLDCESQVGEIKVDPGASSSIRTDWIFQNDIIHTSISLDSAIIDK
                     AGTYYFSSQDQITGCRDTVSFLVGDNSVPIDTVTYRLIGGCQENGSALILDYPIGGTP
                     PYSYSLDNSSFTSYTQFYDLNAGLHQLRVLDSKGCRFLLDFQVDDFVSGSLHLGRDTT
                     IQLGDEIWVQVDFPQELIEWIGVSLNGTPTVIKPEGEWLKPIETTTIQVLIRDKNDCL
                     HEASRVIFVENDTKLFMPNIFSPNADGRNDVFFVPANRKVEMIMELHIYDRWGNQVFS
                     LSNFVPGEEANGWDGTFHGRKMNPGVFVYYVRYRTIDGKEALAKGDFTLLR"
     gene            complement(2618..4399)
                     /locus_tag="PLG24_08595"
     CDS             complement(2618..4399)
                     /locus_tag="PLG24_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HMZ24990.1"
                     /translation="MRYLSALLFLVLFHFSGKGQCVFNLNDTIVADADFYIQGSVSGL
                     VNDDLSANQALCGVKLQFRHQFIGNLQIELVSPAGQKILLVGPPLAGNLTNGTLWDIT
                     FVRCIDIPSPDPAIPPQYDNGSPWAIGATYSGSYFPYAGCLEDFNVGSANGIWQLHII
                     NGSFYEGNLVSFSLIFCDPTGVDCNPCKANPGKFTLNERSFCQNDVNLLSFQPPIDYT
                     PDGQNTTLYNYEYVVTFNGIIQRIEYVNDWSVLSPGTYLIYNIAYLKEYESVVKSYIG
                     DPYSVLLNKINSTQICADVTGSAMQLNIFRVFDNPVRNILLCSGDSLVVSGKTYLADT
                     FIRDTLKSLYGCDSIINIDLRFTNIHINIEADTIDCINFLASIDTSGFRMDVPGGLVL
                     GYEWRDNGGNFISNAGGITVNTPGLYFLYMNILYGNLQCEYLIPKKVESQVFPPERPT
                     ILPFSPCSEYTLKVLLSADTLRDEAHWSITGTSDFRRIGDTLFVTWHQTGTFKVCVWA
                     TNECGTSDTLCQDVTVGQKPVFDILTDSISCNGLFHIEVKSQFQNLTWVDIQGNSIFS
                     VNGDGSLVAGSILVGSGVEIQWEYCRM"
     gene            complement(5108..>5546)
                     /locus_tag="PLG24_08600"
     CDS             complement(5108..>5546)
                     /locus_tag="PLG24_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ24991.1"
                     /translation="EIQFDVFHDCRVKDVIRILSAYRFKEGDSYSKSEMFFENDTYPI
                     ATEYLPFKLAGSKNIAPEKVESFPNPASNEVNMSFSSNISGKFPVTIYDNAGKSVGET
                     IVEVKSGDNKLTFNKALFGSAGSYIIQLNLGNRILTSRATFVN"
BASE COUNT         1781 a         1241 c         1000 g         1524 t
ORIGIN      
        1 aggtgcttta agctggaggc caggggagga tatggtcgcc ggattttcta actatggcgc
       61 aaaaaatgtt gacctgttcg ctcccggagt cgatctgtat tcaactgttc ccaatcaaag
      121 ctacaaaaag gaaagtggta caagtatggc tgctcctgta gtggcaggtg tcgcagtttt
      181 gcttcgctcc tatttcccta cactatctgc taagcaaacc aaggaaatca tattgcaatc
      241 cgtccataag gtgaatgcta tggtgaagaa accaggcact gatgaattgg ttcctttcag
      301 cagcttgagc gtcaccggtg ggtatgtcga tgcttatgaa gcgatcaaac tggccgcaaa
      361 aaaaaccggg gttaaattag caatgcctta ataattcaag catataagga cttgaaactt
      421 ggtccttcct ggaatcagaa agccggcatg cccggctttt tttattgacc attggatcct
      481 aagccactga attccctacc tcaacaaagt aaaatcacct tttgccaaag cttccttacc
      541 atcaatagta cggtacctga cataataaac aaaaacacca gggttcatct ttctgccatg
      601 aaaggtacca tcccatccat tagcttcttc gcccggaaca aagttgctta aactgaatac
      661 ctgattgccc catctgtcat agatatgtaa ttccataatc atctcaacct tccggttagc
      721 cggaacaaag aatacatcat tacgcccatc agcattgggc gaaaaaatat tgggcataaa
      781 caacttcgta tcattctcga caaatataac ccgacttgct tcatgcaggc aatcattctt
      841 gtcgcggata agaacctgaa ttgttgtcgt ttcaattggc ttcagccact ccccttccgg
      901 cttgataacc gtcggagtcc cattgagact gactcctatc cattcaatta actcttgcgg
      961 aaagtcaact tgaacccaaa tctcatctcc taactggatg gttgtatcac gccccagatg
     1021 aagacttcct gaaacaaaat catctacctg gaaatcaagc aggaatcgac accccttact
     1081 atccaacacc cgaagttggt gaagacctgc atttaaatca tagaattgtg tataggaagt
     1141 aaatgaggaa ttatccaggc tgtaactgta aggtggagtc cctccaatag gataatccag
     1201 aattaatgca cttccatttt cttgacaacc tccaatcaaa cgataagtaa cggtatctat
     1261 agggacagaa ttatcaccga ccaaaaaaga aaccgtatcc cggcatcccg taatctgatc
     1321 ctgagatgaa aaataatagg tacctgcctt atcaataatc gccgaatcca aactaataga
     1381 ggtatgtata atatcattct gaaagatcca atcagtgcga attgaagatg aagccccggg
     1441 atctactttt atttccccga cctgagactc acagtccaga taatttgttc cggataccaa
     1501 tatttccgga agcggatata ccttgatggt aagactcgca gtatctgaag gacaaacatc
     1561 acttccggcg acaatcttat ccaacatata atctcccgga aaaagctttt tcgtactgat
     1621 agtattcgtc tgtggatcaa aagctatcga aaatccgggg taatcaggtt tttgagcctt
     1681 ccaggctcca tcattcaaag aattgatcag caaggtatcc agatctatta aataatcctc
     1741 gccattacat atgtccatca aataatcgtc accggcataa aaacgaggaa caacctgcaa
     1801 ctcaaacaaa atggtatctg cacaagcacc ctcccaatcc gctacagtct gcaataggat
     1861 agaatgtaca ccgacaaact ggtcattaat aaggagattc gatccaccaa tggcctcttt
     1921 gctcccggaa gtaaggtcaa accattgtac cggaacatta actccggcca atgaatccag
     1981 attcaaagac ccataattac tgcaaatcct gtaggtatcc ttcagtttgt tcagcgcagg
     2041 tctgttgcag gaacaatcaa ccaccttgaa tacagtcgca tatgtaacat ccttacaagg
     2101 cagtacagct gtgttcgttc taaaattaac cttgtaagtc ccgacagcta catgcgacat
     2161 atctatcgaa tcattggaaa tggtaatacc tgacaatgaa ctcagattcc aatttccgct
     2221 cctttctccg gatacaatca aatctggaag gaaataaacc ggctttccat ccctgttgta
     2281 agtattacat agagtaattg tatcaggaag caatgactcc ggcatatttt caatcctgac
     2341 atcaagcgta tccgattgca gatcgttaca ggttctgtat tgcgttttca aggaatcaat
     2401 atacactcca cccgatgcat taaaatccag atctatataa ttgagtccga tatcaaccgg
     2461 catatgaaaa ccttgaccct caaaagaata atacaattcg ccggcgtgat tcacaatttc
     2521 caccggaata cggtaagtac ctgatccgca gaatgccgta tcctttacta taaacttagg
     2581 gggctcctgt ctgtaaaatt taaggctgcc aaacaaatca catcctgcaa tactcccact
     2641 gtatttcaac accgcttccc accagaatcg atcccgccac aagtgatcca tctccattaa
     2701 cggaaaatat tgaattccct tgtatatcca cccaggtcag attctgaaat tgacttttta
     2761 cttcaatgtg gaacaatcca ttgcaagaaa ttgaatcggt aagaatatca aaaaccggtt
     2821 tctgaccgac cgtgacatcc tgacaaagcg tgtcggatgt gccacattca ttcgtcgccc
     2881 atacacagac cttgaaagtt cccgtttgat gccacgtcac aaaaagcgta tcccctatcc
     2941 tcctgaaatc tgatgtcccg gtaatcgacc aatgcgcttc atccctcaac gtatcggcag
     3001 ataataaaac tttaagggta tattctgaac aaggagaaaa agggagtatc gtaggcctct
     3061 ctggcggaaa aacctgtgat tcaacttttt tagggatgag atattcacat tgaaggttgc
     3121 catacaggat attcatataa agaaaatata atcccggtgt attgaccgtg atgccaccgg
     3181 cattggagat gaagttgccg ccattatccc tccattcata tccgagaact aagcctccag
     3241 gcacatccat tcgaaatccg gaagtatcaa tcgaagccag gaagttgatg caatcgatag
     3301 tgtctgcttc aatattaata tgtatattgg taaacctcaa atcaatatta atgatactgt
     3361 cgcaaccata taatgactta agcgtatctc ttatgaaggt atcagccaaa taagtcttac
     3421 ccgataccac cagactatcc ccactacaaa gcaggatgtt ccttacaggg ttatcaaaga
     3481 ccctgaatat attcaactgc attgcactgc cggtaacatc agcacaaatt tgggtagaat
     3541 tgattttatt caataaaaca ctgtaaggat ctcctatata ggattttact actgactcat
     3601 attctttcag ataagcaata ttataaatga ggtaggtacc gggacttagg acagaccagt
     3661 cattcacata ttctatcctt tgaataatcc cattgaaggt aacaacatat tcatagttgt
     3721 ataatgtcgt attctggcca tcaggcgtat aatcgatagg cggctgaaat gaaagtaaat
     3781 taacatcatt ttgacagaaa gacctttcat tcaaggtaaa ttttccagga ttggctttgc
     3841 atggattaca atcaacgccg gtagggtcac aaaaaataag actaaaactg acaaggttac
     3901 cttcgtaaaa agacccatta ataatatgca actgccaaat accatttgca gaccccacat
     3961 tgaaatcctc aagacatccg gcatatggaa aatatgaacc ggaataagtt gccccaattg
     4021 cccagggtga accattgtca tattgcggtg gaattgctgg atccggtgat gggatatcaa
     4081 tacacctgac aaaggtaatg tcccataatg taccattggt caaattgccc gccaaaggtg
     4141 gccctaccag tagtatcttt tgtccggcag gagaaactaa ttcaatttga aggttcccga
     4201 tgaattggtg tctgaattga agcttgaccc cacaaagcgc ctgattggca gaaagatcat
     4261 catttaccag tccgctgaca cttccctgga tataaaaatc cgcatccgcc acaatagtgt
     4321 cattgaggtt gaatacgcat tggccttttc cggaaaaatg aaaaagaacc aaaaacaaaa
     4381 gtgcagaaag gtatctcaac ttttgtatga ttatgggatt aataagcaaa tatacatcta
     4441 atttcaacga tttgggtaaa gcaaccctgt taatgacttt tatccacagc ctttaaatta
     4501 tcatttttca aaagcatcaa tataataatg tatatcccaa agatagatta ggctgctttt
     4561 ccttataaaa aataacttaa gttttcacat tactctgact atgttactga tcaattatgt
     4621 tcctttcatg agacctgaat cacctgataa attgttccta tagcaaggag ataatgtcaa
     4681 aggaatggtt ccaacttcaa gggatcatga tcccatgaca accaatgtat ttttttgaat
     4741 actcactatt tacttaattt cagaaaaagg aataacaaaa caaggccttg tcatgtcatt
     4801 gaattcctta tacggttgag agatatgggt gtcgtttgcg ggcctaagag aatttctcct
     4861 acaataaaaa ctggcacctg caagaatttt ccattagcag ataaaaagac agggtacatt
     4921 agttgaccgg tttaaattgt tgtcggataa aacacttttg ccccgtcaac cttaaagatc
     4981 ttgaattagt aaaatgtaat tggtaaagtg taaaacagga gaaatataaa aaaggaactt
     5041 ccaaatggca gttccttttc taacagattt gattaaaaat tcagattatt cctacaagtt
     5101 gatctgatta attcacaaag gtcgccctgg aggtaaggat gcgattacca agattcaact
     5161 gaataatata actccccgca gaaccaaaca gtgctttgtt gaatgtcagc ttattatccc
     5221 ctgatttaac ttcaactatc gtctctccta ccgatttgcc ggcattatca taaatagtca
     5281 ccggaaactt tcctgatata ttagaagaaa aactcatatt aacttcattg gatgcaggat
     5341 ttggaaacga ttccactttt tccggagcga tattcttgct tccggctaac ttaaacggaa
     5401 ggtattctgt tgcaatcgga tatgtatcat tttcaaagaa catttcactt ttactatagg
     5461 aatccccttc cttaaaacga taggcagaaa gaatcctgat tacatcctta acgcgacaat
     5521 catgaaaaac gtcaaattgt atctca
//
LOCUS       DAOQQN010000214         5543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6510_length_5543_cov_4.793003, whole genome shotgun sequence.
ACCESSION   DAOQQN010000214 DAOQQN010000000
VERSION     DAOQQN010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6510_length_5543_cov_4.793003"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..893
                     /gene="pyrF"
                     /locus_tag="PLG24_08605"
     CDS             93..893
                     /gene="pyrF"
                     /locus_tag="PLG24_08605"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HMZ24992.1"
                     /translation="MNQRQLTELIRSKGSFLCVGLDPDMSRIPAPYSDADDSLFEFNK
                     MVIDATRDHCVAYKPNTAFFEAHGASGWLSLEKTIRYIGSSHFVIADAKRGDIGNTAN
                     MYAEAFFKELDVDAITLSPYMGHDSVSPYLKYPDKWAIILVKTSNTGSSDFQTLVTEN
                     DSPLYIDVLRKCLAWGDIDNTMFVVGANNLSELKGIREIAPDHFFLVPGFGAQGGQLK
                     DIQPYLSSDTVGILANYSRQVIYAGDFAQINEAAAAIHRQMREMLKTL"
     gene            1312..1854
                     /locus_tag="PLG24_08610"
     CDS             1312..1854
                     /locus_tag="PLG24_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMZ24993.1"
                     /translation="MTDEQLVEMLLDKARQEQGFRMLMHQYRERLYSVIRKIVLTHEN
                     ADDVFQNTMIKIFTHIGQFGNKSSLFTWMYRIATNEALSFLRRQKKVGADGEVKPELT
                     EMWLQADPFFDGDELQIELMKAVALLPSKQKEVFNLRYFDEMKYSDLSELLGVSEGAL
                     KATYYHATKKIEAYFSQLNK"
     gene            1859..2299
                     /locus_tag="PLG24_08615"
     CDS             1859..2299
                     /locus_tag="PLG24_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24994.1"
                     /translation="MEKDIKNLRKDNFRIPDGYFDEVQMKISARLEQGNGRILPLESG
                     KGLSPGIVKIRKLVFIMGVAAACLAGFIIFKPQSVPAHEINLNGISNEDIATYLKENV
                     MDIDLEMIANTDPTLTATSNNPDNLSDEEYDLLIDEISLDDIID"
     gene            2405..2758
                     /locus_tag="PLG24_08620"
     CDS             2405..2758
                     /locus_tag="PLG24_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24995.1"
                     /translation="MERIQAQRVAYITNELSLTPAESEKFWPVYKEYKQKVHQLNQSQ
                     RPSKPIEQMSDAEKTSFINTMLDNEIKAAEIKRDYYVSFQKILPVAKVAKLEMAERSF
                     KKELIGKMAGENRRR"
     gene            complement(3007..4407)
                     /locus_tag="PLG24_08625"
     CDS             complement(3007..4407)
                     /locus_tag="PLG24_08625"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HMZ24996.1"
                     /translation="MNNTRKRDLLVYGICDALMAFTAWAIFSYKFVINRNFVLLENPT
                     LQAWGTAGLIVVGFILLYTIFDFYTDLYRQSRLATLTRTIFISLLGCLLVVLLIRDVF
                     MGNTISLWFHFFLWHFFLTALSRMILLTRASRRLKAGSVTFNTIIIGGDENALELYTE
                     IKSRQKGLGYKFIGFIDSNGSSRNLLEGHLPLLGKLDQLAAIIVQHKVEEVIIAIETQ
                     EHDKLRDILNVLFDFSSNILIKVIPSMYDIMLGTVKMNHVYGAVLIEIEQVMMPKWQV
                     LVKRGLDIVISIIVIVLLSPMYLYIYIRVKMSSPGPAIFLQERIGLNHRPFSIYKFRS
                     MYTNAEEDGPQLSNENDARCTPWGAVMRKWRLDELPQFFNVLKGEMSIVGPRPERRFY
                     IEKIMERAPHYKHLLKVRPGITSWGQVKYGYASNVEQMIQRLKFDILYIENMSLALDF
                     KIMFYTLLVLFQGKGK"
     gene            complement(4414..5340)
                     /locus_tag="PLG24_08630"
     CDS             complement(4414..5340)
                     /locus_tag="PLG24_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24997.1"
                     /translation="MINFSPEYDILFYTFAHSFEMRFHITGTYLLTTILLTTLAGCSK
                     EESSVPTYLSFGQANIIFQQNAGSNHQNIEDYWLIINGQTIAVASPDRTVPVIGNGIQ
                     EITVQPGIRVNGQRDEAQIYSFLEPYKTTVSLCACADTIHLIPEFKYKPTALFSFIED
                     FENSNRFSVDLDDNIATSSTTQTQTVAEGSFSLALNVDATNKINSVTTQDFYTDFESN
                     GRDLFLEFEYKSDFTFLIGLQANTGSGHFTEYAAGAYATPTWKKMYVNFTDLALASKS
                     TGGYRPVFTINHAGGTQGGGNLYLDNIKLVHY"
BASE COUNT         1685 a         1037 c         1186 g         1635 t
ORIGIN      
        1 ggtggggtgg aacaggtcct taaaacccgg gttcccgaga tcataagtat agaagtagaa
       61 ggcgcgctct gattcaagaa taaaaatgtg atgtgaacca acggcaatta accgaactca
      121 tacgaagcaa agggagtttt ctttgtgttg gattggatcc ggatatgtcc agaatacccg
      181 caccttattc agatgcagat gattctttat ttgagtttaa caaaatggtc attgatgcta
      241 caagagacca ttgcgtggcc tacaagccta acacagcttt tttcgaggct catggggctt
      301 caggttggtt aagtcttgaa aagacaattc ggtatattgg cagttcacac tttgtaattg
      361 ccgatgcaaa gcgtggcgac ataggcaata ctgcaaatat gtatgcagag gcgtttttca
      421 aggagttgga tgttgacgca atcacacttt cgccttacat gggccacgat tctgtgagtc
      481 cttacctgaa gtatccggac aagtgggcca ttattctagt caagacatcc aataccggta
      541 gcagtgactt tcagacgtta gtcactgaga atgactcacc actttatatc gatgtactga
      601 ggaaatgcct tgcatggggc gatatcgaca atacgatgtt tgtcgttgga gcgaacaatc
      661 tgtcagaact gaaagggatc agggagattg cacctgacca tttttttctg gtccccggat
      721 ttggcgcaca aggcgggcag ttaaaagaca tccagccgta tctttcttca gatacagtgg
      781 gcattttggc caattacagc cgtcaggtaa tttatgccgg agattttgca caaatcaatg
      841 aagccgcagc cgccatccat agacaaatga gagagatgct caagactctt tgaaagcagc
      901 aaccccgtaa tacattaatt taataggaat ctttaggata ttgaagaatt cttctactat
      961 gcaagaattg gtctggaaat acaagaaacc ttttcgtttt cctggtgtat cgttgagcca
     1021 agaaatttta agagatcatg acacatgtta ttgggtgttg tgtatctttt atctgtatta
     1081 atatgggcta atttatttta gatgaggagg acttctacgg ttttattttc ttccaattgt
     1141 ggattaaagg ctgttatcat tgaatttgcc ctctgtttta acacctattt tcttacattt
     1201 atttttaata attaaacctt ttagagaaat ccgcatcaaa tggttgtgcg tctgaagaaa
     1261 aagtgacaat caaaaagcga gattgagttg ataaatctat tagagaatac aatgactgat
     1321 gagcagctgg tagaaatgct acttgataag gcccggcagg aacaagggtt tcggatgctg
     1381 atgcatcaat atagagagcg actgtacagt gtcattcgta agatagtgtt gactcatgaa
     1441 aatgccgatg atgtatttca gaatacgatg atcaagatat tcacccatat cggccagttt
     1501 ggaaataaat cgtcactttt tacatggatg taccggattg cgactaatga ggcattgtcg
     1561 tttttaagaa ggcagaaaaa ggtgggtgct gatggagagg ttaagccgga gttgaccgaa
     1621 atgtggttac aggcagatcc gtttttcgat ggtgatgaac ttcagattga attgatgaag
     1681 gcagtcgcat tattgccgag taagcagaaa gaggttttca acctgagata ctttgatgag
     1741 atgaaataca gcgatttaag tgaattgttg ggagtaagtg aaggtgcttt gaaagctact
     1801 tattaccatg caactaaaaa aatagaggct tattttagcc aactaaataa atgagaatat
     1861 ggaaaaagat ataaaaaatt tgagaaagga taacttcagg atacctgatg ggtattttga
     1921 tgaggtgcag atgaagatat ctgcacgtct tgaacaaggt aacggcagga tattgccctt
     1981 agagtccggg aagggtttaa gtccgggaat agtaaagatt cggaagttgg tttttattat
     2041 gggggtagca gctgcttgcc tggccgggtt tataattttc aaaccacaga gtgtcccggc
     2101 acatgaaatc aatttgaatg gtatctccaa tgaagatatt gctacatatt taaaggaaaa
     2161 tgtgatggac atcgatctgg agatgattgc aaacaccgat ccaaccttga cagctacatc
     2221 aaacaatccg gacaatcttt ccgatgaaga atacgatctt ctgatagatg aaatttcact
     2281 cgacgatatt attgactaaa tttcttaact tttaaatttt attaaattga aaaccaaact
     2341 atcatttttt gtgatttgca ttttatttac ctctttttct gtttatgcac agaaaggtaa
     2401 aggaatggaa aggatacagg cacagagagt tgcctatatc accaacgagt tatcattgac
     2461 tccggctgaa tcagaaaaat tctggccggt ttataaggaa tacaagcaaa aagtacatca
     2521 attgaatcag agccaaagac cttccaagcc gatagaacaa atgagtgatg ctgaaaagac
     2581 aagttttatc aacactatgc tggataatga aattaaagca gccgaaatca agcgggatta
     2641 ctacgtaagt tttcaaaaaa ttcttcctgt agctaaagtt gcgaaactgg aaatggcaga
     2701 acgttccttt aagaaagaat tgattggcaa gatggcagga gaaaatcgaa gaagataatt
     2761 cttattttat tgaaatgcaa tgaataatgg agcatagggt cgggagatct tgtgctccgt
     2821 ttttttgttg caaccgaccg tttgtcattt caagccgcaa tcggtatcac cgttacaact
     2881 catccatagg atttataaat tttatggttg tccatccgga tcacaagtcg ttgtgaaatt
     2941 aaaattacgg agtggactct tctcttcagt ttttgaataa gaaattttcc aatttcactc
     3001 tactgactat ttccctttcc cctgaaaaag taccagcaag gtatagaaca tgattttaaa
     3061 atccagggcc agagacatat tctcaatgta tagaatgtcg aatttgagtc tttggatcat
     3121 ctgttccaca ttggatgcgt agccgtattt gacctgtccc caggaagtga tacccggtct
     3181 gacttttagc agatgcttat agtgaggtgc cctttccata atcttttcaa tataaaaacg
     3241 cctttcaggt cgtgggccga cgatggacat ttcgcccttg aggacattga agaactgtgg
     3301 aagctcatca agacgccatt ttcgcatgac agctccccat ggtgtgcatc gggcgtcatt
     3361 ttcattactg agttgggggc cgtcttcttc ggcattggtg tacatcgacc tgaatttata
     3421 gatagaaaaa ggacgatggt ttagacctat cctttcctgt aagaatattg ccgggcccgg
     3481 actgctcatt tttacccgaa tatatatata caaatacata ggactgagca gtacaatgac
     3541 aatgatgctg atgacgatgt ccaggccccg tttgacaagt acctgccact taggcatcat
     3601 aacctgctct atttctatca gaaccgcgcc atatacatgg ttcattttta cagtgcctaa
     3661 cattatgtcg tacatgctag ggatgacctt aatcaggata ttagaactga aatcgaacag
     3721 aacgttgagg atatctcgca gcttgtcatg ttcctgagtt tcaatagcta tgatgacttc
     3781 ctctacctta tgctgcacga tgatggctgc caattgatcc aacttgccca gtaatggcag
     3841 gtggccttct aacagattcc tggaggagcc attactgtca ataaaaccta tgaacttata
     3901 acctaatcct ttttgtctgc ttttaatttc ggtatatagt tccagcgcgt tttcatcccc
     3961 tccaatgatg atggtattga aggtaacgga ccctgctttg agtcttctgc tggcacgggt
     4021 aagcagaatc attctactca gcgccgtcag gaaaaaatgc caaaggaaaa aatggaacca
     4081 caaggaaatc gtattcccca tgaaaacatc tctgatcagc aaaaccacaa gaagacaacc
     4141 aagcagggaa ataaatatcg tacgtgtaag agttgccagt cttgattgcc ggtaaagatc
     4201 ggtatagaag tcaaaaatgg tgtatagcag gatgaaaccc acaacaatca agcctgcagt
     4261 gccccaagct tgcaatgttg gattttccag taaaacaaag ttacggttga tcacaaattt
     4321 gtacgagaaa atagcccagg cagtgaatgc catcaaggca tcgcaaatgc cataaaccaa
     4381 taagtcccgt tttctcgtat tgttcataac cgattaataa tgaactagtt taatgttgtc
     4441 taagtataaa ttccctccac cttgcgtgcc accggcgtga ttgatggtaa ataccggcct
     4501 gtacccaccc gtactcttgg atgccagggc gagatcggta aaattgacat acattttctt
     4561 ccatgttggt gtggcataag caccggcagc atattcagta aagtgtccgg aaccggtatt
     4621 ggcttgcaat ccgatcagaa aggtgaaatc acttttatat tcgaattcca gaaaaagatc
     4681 acgtccatta gattcgaaat cagtataaaa atcctgggtt gtgaccgagt tgatcttatt
     4741 ggtagcatct acattcaagg caagactaaa gctaccttct gctacagttt gtgtttgagt
     4801 cgttgacgat gtcgctatgt tgtcatcaag atcaaccgaa aaccggttgc tgttctcaaa
     4861 atcttcgatg aagctaaaca atgcagtagg cttatatttg aattccggaa ttaaatggat
     4921 ggtatcggca catgcgcaca atgacacagt tgttttatat ggctccagga aggaatatat
     4981 ttgagcttca tccctttgac cattcactct gatccccggc tgaaccgtga tttcctgtat
     5041 cccgttgcca ataacaggta cggtacgatc cggtgatgcc acagcgattg tttggccatt
     5101 aataatcaac cagtaatctt ctatattttg gtgattactt cctgcatttt gttgaaaaat
     5161 gatatttgcc tggccaaaac taagataggt tggtactgaa ctttcttcct tactgcatcc
     5221 cgccagggta gtgaggagta tcgtggttag caaatatgtt cctgtaatat gaaatctcat
     5281 ctcaaatgaa tgcgcaaaag tataaaataa aatgtcatat tccggactaa aatttatcat
     5341 tccatctttg tgctaatttt tcttgtatat tgtaactaat attattattt gtaactttgc
     5401 agccgatttt tgaaaccttg tttctttcca attgtattat atggctaaag tcatttgaca
     5461 atgaggttaa gaatgagtgg gaaacaatca aaaaatgaaa atttgtaaaa agatattaaa
     5521 tgaattttta tcttaatatg ttc
//
LOCUS       DAOQQN010000215         5535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6519_length_5535_cov_4.418248, whole genome shotgun sequence.
ACCESSION   DAOQQN010000215 DAOQQN010000000
VERSION     DAOQQN010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6519_length_5535_cov_4.418248"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PLG24_08635"
     CDS             <1..203
                     /locus_tag="PLG24_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ24998.1"
                     /translation="GGYGLQSVLLLSLVYGVCSILSIMLMIYLVYKGSKIFANNEFIE
                     HNEKRITGLVLIIVGLLSFFLH"
     gene            213..1073
                     /locus_tag="PLG24_08640"
     CDS             213..1073
                     /locus_tag="PLG24_08640"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HMZ24999.1"
                     /translation="MKRSVLFLFSFFSFLHLYGQKASIHGADVSTSDTLGITSFNIRY
                     GEANDGINSWPNRKSLIKNYVQSTRPDILGVQEALIGQIAYLDSIPGYEFVGVGRDNG
                     MTEGEYCAIFYNADELEVRESGTFWLSETPGVPTKGWDGACPRICTWAKFKNKNGKEF
                     CVFNTHLDHMGRKARMEGARLIISKINLLNPRIPVFLIGDFNATSEDLPIRIISDFLA
                     DSREISQFPDTTDGFTFNGFKEEGQATERIDYIFVNNFVRVISFHTNQLRPDNKYLSD
                     HYPIEGTFIY"
     gene            complement(1463..2800)
                     /locus_tag="PLG24_08645"
     CDS             complement(1463..2800)
                     /locus_tag="PLG24_08645"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HMZ25000.1"
                     /translation="MGSVIIRNARIVNEGTIIEGDVKVINGYFEQIGRIRHEGDTPEI
                     DFEGDYLIPGIIDDQVHFRQPGLTHKADIRTESMAAAAGGVTTFMEMPNTYPPATTLE
                     LLEDKYSIAEEASIVNYSFFLGASNNNLDEILKADYSQICGVKIFMGSSTGDLLVDNE
                     TALENIFENCPALIATHCEDEATIRQNMEEYIAKYGDNLDAHFHPLIRSAEGCYLSSS
                     RAINLAKKHNSRLHILHISTGKETELFGNDVPLKNKRITAEACVHHLYFSDRDYTRLG
                     NKIKCNPAIKTQEDREAIFKALKSGKLDIVATDHAPHTLDEKNQPYLKAPSGLPLVQH
                     SLNIMLSFVRKEKATLEEVVHWMCHAPAICFRIKGRGYIREGYHADFVRLNLSTNTLV
                     DEESLFYKCGWSPLEGESLPGKVISTWVNGVKVYEDGIIITNQGKRVEFEVDY"
     gene            complement(2896..3828)
                     /gene="mdh"
                     /locus_tag="PLG24_08650"
     CDS             complement(2896..3828)
                     /gene="mdh"
                     /locus_tag="PLG24_08650"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HMZ25001.1"
                     /translation="MSKVTVVGAGNVGATVANVLAHGDFVNEVVLLDIKGEMAQGKAL
                     DSWQQAPIDGYSTFLIGSSDYADTANSDVVVITAGLPRKPGMSRDDLISTNAGIVNGV
                     TKSILQYSPNAIIIVVSNPLDVMTYAAHKTSGLSSSRVIGMAGILDTARYRAFLAEEL
                     KVSPKDIQAILMGGHGDTMVPLPRYTTVGGIPVTELVAEEKLNAIVERTKKGGGELVQ
                     LMGTSAWYAPGAAAAQMVTAILKDERRIFPCCIKVEGHYGIDGVYVGVPVVLGRNGVE
                     KVIELDLNESEKALLAESTAAVKSVMDVYDGMGL"
     gene            3976..5523
                     /locus_tag="PLG24_08655"
     CDS             3976..5523
                     /locus_tag="PLG24_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ25002.1"
                     /translation="MRIFLTTSWLSLFFIFCHTLEAQQVRGYCGTQSSPVADAQLDAN
                     IAYLAAHPDVLNTRGITYVPVRFTIVRSASGNGGVNPKSVFRMFCKLNEDYLDQDIQF
                     YMKDDFKYLNNDLANTDPQSSSGGSILKNVKDKTALNIFFVEEIKSSSPGSVLGFFSP
                     ENDYLVVRNSEANYESQTTSHEMGHFFSLRHTFYGWECEPWNVNDHGQIVTFTIAPCF
                     SAQVELVNGSNCSTAADKVCDTPPDYNFGFGWPNDCRPFNVDVYDKNNDLIRPSQNNF
                     MSYFFGCTGWHFSQGQKNLIRTDLNSSKRSYLKSTYTPPSTTVSGEVNYQYPINNQEA
                     LRNAPIVLQWENVPGATFYIVEYSRRKSFDSNFTVTEMVRTNQVTIPANYFPSGAATG
                     YWRVIPMNEAVTCNVPANGSEQSFTLVNALGTKSIDGLQHFVVKPNVLRSGNSIQVEV
                     SAGKSMDCMVQITDINGRVVYHYGQYKMNLGMNKIVLDEINLSTGIYIVSIKNKSGIN
                     SQKLIVL"
BASE COUNT         1500 a         1102 c         1176 g         1757 t
ORIGIN      
        1 caggtggcta cggtcttcaa agtgtactgt tgttgtcgct ggtttatggt gtttgcagta
       61 ttctttcgat tatgcttatg atttatctgg tctataaggg aagcaagata tttgccaata
      121 atgaatttat tgaacacaat gaaaagcgaa ttaccggatt ggtattgatt atcgtaggct
      181 tattgtcttt ctttttacat taaatgataa aaatgaagcg ttctgtctta ttcttgttct
      241 catttttttc atttctgcac ctttatgggc agaaagcctc catacatggg gctgatgttt
      301 ctacatccga tacgctcggg attacttcct ttaatattcg ttatggtgag gccaatgatg
      361 ggatcaatag ttggcctaat agaaagtcat tgattaaaaa ttatgtacag tcaacccgtc
      421 ctgatatcct gggagtgcag gaagcactga ttggacaaat agcgtatctt gactcaatac
      481 cgggttatga attcgtcggt gttggccgtg acaatggaat gacagaaggt gaatattgtg
      541 ccatattcta taatgccgat gaattagaag tcagagagtc ggggacgttc tggttatctg
      601 agacgccggg tgttcctaca aagggatggg atggtgcgtg cccccgtatt tgtacctggg
      661 cgaaatttaa aaataaaaat ggaaaagagt tttgtgtatt taatacgcat ctggaccata
      721 tgggccgcaa ggcgaggatg gaaggagcaa gactgatcat ttcaaaaatt aacttgttga
      781 atcccaggat tccggtcttc ctcatcggtg actttaatgc gacttccgag gatttgccta
      841 tcagaatcat ttcggatttt ttagctgaca gcagggagat ttctcaattt ccggacacca
      901 cggatggttt taccttcaat ggctttaagg aagaaggaca ggcaacagag cgcattgatt
      961 acatcttcgt aaataatttt gttcgtgtga tctctttcca taccaaccaa ctgcggccgg
     1021 acaataaata tctttctgac cattatccaa ttgaaggtac ttttatttat taaaagtttt
     1081 tggaaacaga acatttattt tgatttagca agacgctatt atactgtaag cattgtgatt
     1141 ttttgagcat cattgtcaag gccaactgct aatgctacat ttttattctc atgctccggg
     1201 tatttcaaat tctgaaattt aggatgcaat taaagtcatc cttgttacta ttgtgattcc
     1261 gacaatcctt caacagactc ttgcaactgt tgataaagct attggggttg tgggtgatga
     1321 gcagtaaggc attggattga aatagttcgg aggcgcaatg atgggaaata aggcagataa
     1381 tttcaaaaga aattcaggca attcccgttg aatttatacg gataatttat tccctcaggt
     1441 gattttatat tggtatcgga tttcaatagt caacctcaaa ttctactctc tttccctgat
     1501 tggttatgat gatgccatct tcgtatactt tgacgccatt tacccaggtt gagattactt
     1561 tgcctggaag agattcgcct tccaaaggtg accatccaca tttgtagaac agggattctt
     1621 catcaaccaa tgtgtttgta ctcaggttga ggcgaacaaa atctgcatgg tacccttcgc
     1681 ggatataccc tcttcctttt attctaaagc aaatagccgg ggcgtggcac atccagtgga
     1741 caacttcttc cagggtggct ttttccttcc tgacaaaact aagcatgata ttaaggctgt
     1801 gttggaccaa tggaagtcct gaaggggctt tcaaataggg ctgatttttt tcatccagcg
     1861 tatgaggggc gtggtcggtg gccactatat ccagttttcc tgactttaat gctttaaata
     1921 ttgcctctct gtcttcctgt gttttaatgg cgggattaca ttttatttta ttgcctaatc
     1981 gggtataatc cctgtctgaa aagtaaagat ggtgtacaca ggcctctgca gtgattcttt
     2041 tgtttttgag aggcacatca ttgccaaata gctccgtctc cttgccggta ctgatgtgca
     2101 ggatatgcag tcgtgagttg tgtttctttg caagatttat cgccctggaa gatgaaaggt
     2161 agcatccttc tgcacttcga atcaatggat gaaaatgagc atccaggttg tcaccatatt
     2221 ttgctatgta ttcctccata ttttgtctga tagtagcttc atcctcgcaa tgtgttgcta
     2281 tcaacgccgg acaattttca aaaatatttt ccagggcagt ttcattatcg actaaaaggt
     2341 cgccggtact gcttcccata aatattttga caccacagat ttgagagtag tcggctttca
     2401 ggatttcgtc caaattatta ttacttgctc cgagaaaaaa ggagtaattg acaatacttg
     2461 cctcttcagc tatgctgtat ttatcttcaa gtaattccag ggtggttgcc ggaggatagg
     2521 tattgggcat ttccatgaag gtggtgactc ctcctgctgc ggctgccatt gattctgtcc
     2581 tgatatcggc tttatgagtc aatccgggtt gtctgaagtg gacttggtca tcaatgatac
     2641 ccgggatgag ataatcacct tcaaagtcaa tttcaggcgt atcaccttca tgccggattc
     2701 tgccaatttg ttcaaaatag ccattgatga ctttgacatc tccttctata attgtgcctt
     2761 cgttgactat ccgtgcattc ctaatgatta ccgatcccat acatttatgc ttagtttgca
     2821 aaaataatgt ttataacgca aaacagccac ccaaataaga gtggctgtcc tggcatatgt
     2881 tatcattgtg gattatcaaa gacccattcc gtcatataca tccatgactg acttgacagc
     2941 agctgtcgat tcagccagga gtgccttctc agactcatta aggtctaact cgattacttt
     3001 ttcaacacca tttcgtccca acacaaccgg aacacctaca tatacaccat cgataccata
     3061 atgtccttct actttgatgc aacaagggaa gatccttctt tcatccttta agatggcagt
     3121 taccatttgt gctgctgccg ctccaggtgc ataccatgct gaagtgccca tcaattgtac
     3181 cagctcacca ccgcctttct tggtcctttc tactatggcg tttagctttt cttcagctac
     3241 cagctcagtt accggaatac ctccgactgt agtgtatcgg ggtagtggaa ccattgtatc
     3301 accgtgacca cccatgagga tagcctggat atctttagga gaaactttga gttcttcagc
     3361 caggaaggca cgatagcgcg ctgtgtctaa tataccggcc attccgatta ccctggatga
     3421 agaaaggccg gaagttttgt gcgcagcata agtcataaca tccaatggat tggaaacaac
     3481 tatgataatg gcgtttggcg aatattgaag aatacttttt gttacgccat tcacgatgcc
     3541 tgcattggtt gagatcaggt catcacggct cataccgggt ttgcgtggaa ggcctgcagt
     3601 aatcactact acatccgaat tcgctgtatc ggcatagtcg cttgagccga tcaaaaaggt
     3661 gctatatcca tcgatgggtg cttgttgcca ggaatcaagg gcttttcctt gtgccatttc
     3721 accttttata tccaataaaa cgacttcatt gacgaaatcg ccatgtgcca atacgttggc
     3781 tacagtggca cccacattgc cggcgccaac tacagttact ttactcattg ctaatattat
     3841 tgatgtttgg aatttaattt caggctgcaa aagtatgact ttaaatagta aataaatagt
     3901 aaattttgtt ttatttaaaa aggcatttcc ggtttttgcg tatcttgcag ctttataatt
     3961 ctaatctcgt tctctatgcg aatatttttg acaacctcct ggttaagttt gttttttatt
     4021 ttttgtcata cgcttgaagc tcagcaggtg cggggctatt gtgggacaca aagcagtcct
     4081 gtggccgatg ctcagctgga tgctaacatt gcctatctgg ccgcacaccc tgatgtattg
     4141 aatactagag gaattactta tgttcccgtt cgatttacga tagtcagatc tgccagtgga
     4201 aatggtggtg tcaacccgaa atcagttttc agaatgttct gtaagttgaa tgaagactat
     4261 ttggatcagg atattcagtt ttatatgaag gatgatttca aatatcttaa caatgatttg
     4321 gcgaataccg atcctcaaag ttcaagtggt ggaagtatcc tgaagaatgt aaaggataaa
     4381 acagcattaa atatcttttt tgtagaagaa atcaaatcca gcagcccggg aagcgttctt
     4441 ggatttttta gtccggagaa tgattacctt gtcgttagaa acagtgaggc taattacgaa
     4501 tctcaaacca cttcacatga gatgggacac tttttcagcc ttcgacatac tttctatggt
     4561 tgggaatgcg aaccgtggaa tgtaaatgac catggccaaa tagtcacttt tacgattgcc
     4621 ccttgtttta gcgctcaggt tgaattagtc aatggttcca attgttctac agcagcagat
     4681 aaggtttgtg atactccacc ggactacaac tttggctttg gctggcctaa tgattgcaga
     4741 ccattcaatg ttgatgtata tgacaaaaat aatgacctga tcaggccctc tcaaaacaac
     4801 tttatgtcat acttttttgg ttgtacaggt tggcatttct ctcagggtca gaaaaacctt
     4861 atcaggactg atctgaatag ttcgaagcgg agttacctga aatctacata cacaccaccc
     4921 tcaactaccg tgagtggtga agtaaactat cagtatccta ttaataacca ggaagcgtta
     4981 agaaatgctc caatcgtact tcaatgggag aatgttcccg gggctacttt ttatatcgta
     5041 gaatattcaa gaagaaagtc atttgattct aattttactg taacagagat ggtaaggacc
     5101 aatcaagtta caattcctgc caattatttt ccatccggag ctgcaacagg ttactggaga
     5161 gtaataccga tgaatgaggc cgttacttgc aatgttccgg ctaatggaag cgagcaatct
     5221 tttacccttg tcaatgcttt aggtactaaa agtattgatg ggctgcagca ttttgttgtc
     5281 aagcccaacg tcttacgctc cggcaattcc attcaagttg aggttagtgc aggcaaatcc
     5341 atggactgta tggttcagat tactgacatt aacggtaggg tagtctatca ttatggacag
     5401 tataagatga atttaggtat gaataagatt gtcttggatg agattaatct gtctaccggg
     5461 atctatatcg tcagtattaa gaacaaatca ggtatcaact cgcaaaagtt gattgtactt
     5521 taatcaagat gtatg
//
LOCUS       DAOQQN010000216         5527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6533_length_5527_cov_6.411001, whole genome shotgun sequence.
ACCESSION   DAOQQN010000216 DAOQQN010000000
VERSION     DAOQQN010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6533_length_5527_cov_6.411001"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..3246)
                     /locus_tag="PLG24_08660"
     CDS             complement(142..3246)
                     /locus_tag="PLG24_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ25003.1"
                     /translation="MKKHQSALLYLLVIALIFNSCASPHKKKEDVGLLGRAYQNMTAY
                     YNGYFNANELLDAAMLEIESKHPDNFQEQLIIYPYLLDQDSTTASGTLNAAIEKLKKD
                     IYLHPASHWVDDSYFQMGKALFLKKDYERAENAFKYVVQNYSEAQLARDKANRMSPGK
                     KKKAIEKERQEVKKEKEAEKEKVREEKADAKKAKEKEAKQKAADRKKAAKERKKAAQK
                     KAKERKKAAAAKKKGKSTPKTAPKTTKAPESKPAGTTTPDSKPTDNKTEVKTEKADTQ
                     PVKSVKEDEDNVVATKKDKPSIRPKNYLFKHRPIFQQAQLWLAKTYIQRSNYEDGERI
                     LRSLLNEGGTYDLVRREALLVSAQVDMKKESYTQAIENLNKAIPMVKEKKRRARLTYI
                     LGQLYIKAGNYDMATQAFERVERLKPSYEMAFNASLSKFGCSVATGKISDEELNSRLN
                     KMLKDLKNDEYQDQIYFMLADINLRNNDIAAGIKNLQKGIQVSKKGPEAKAESYLKLA
                     DIFYKRDQFDLAYHYYDSTATAYPKTKERFKEIVDRRDNLKDIAYNIETIQYQDSLLM
                     VGRMTPEEQEALAKKLIKEAREKEKLATASNTGSTFNPKVGRGGSQPAAIGGGPAFGN
                     PGQIGGAKPTAFFAYNDKTLKDGKRQFDKDWNNRPLADNWRYSALLQSFDNNTSTDKE
                     EGLSALFISKSEIKKVLADVPTDAATIARSEAAIMDAMNALGVLYPDKIERYDKAIEV
                     LEDLLQRFPDNPHEPQAFYQLYFAYMMKGNSQKANYYADLLKTKYNGNQYSLYFSDPQ
                     YIKNKADKEAELNDYYLSTYTSMEKGDYATAFNMAQKSDSIFGIKNILRPKFAMVTAM
                     SLGYLKGKETYISALKDLVQAYPNTMEEKRAKEMLRLLGTGSNANEQQLAEAEKIYKA
                     TDADQHFILIIFKPEITGTEEVRAQVTDYNTRYFSSANLKIATITLKDGNQDRQVLLV
                     RTLDNKIKAMEYYNQALNKKGDFISARFPYDMYAISNANYRELLRLRSAELYDIWFEK
                     YYRN"
     gene            complement(3460..3687)
                     /locus_tag="PLG24_08665"
     CDS             complement(3460..3687)
                     /locus_tag="PLG24_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25004.1"
                     /translation="MHNNWNFLRILRLVLGAIILVEGIRSGEVFFLIFGAIFTLMPLL
                     NVGCCSAAYCNPRQQRTSAYKDEEIHFEEVK"
     gene            complement(3785..3997)
                     /locus_tag="PLG24_08670"
     CDS             complement(3785..3997)
                     /locus_tag="PLG24_08670"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HMZ25005.1"
                     /translation="MKKNLGTGDKFIRLLLAVLILALYLTNAISGNMALLLLFFAIVF
                     ALTSYISFCPIYQIFGINTCKAKEQK"
     gene            4267..>5527
                     /locus_tag="PLG24_08675"
     CDS             4267..>5527
                     /locus_tag="PLG24_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HMZ25006.1"
                     /translation="MNLIIRFFFGIAMIALAHMVNGQSVKPIHTVHGSFTPPPKEVVL
                     QNHESRNPSREKIVWIQLADDTINGFRDSIVNRLSPVDFIDDRTFAVRVDAVGLKDLA
                     ANEAVVSIFEMPAALKLDRKVVDADDSEKLEVRISLYGAMSDSEIENLIGPVKIISHH
                     VSGDKTYLVIACDRPSALEIAAGAQVRAISENISAFTPLNFYKRSLEAVQGANASLQN
                     GGLGLNGAGITIGHGDTGGFFHEDLNDRVVHFALDSVTAHAPHTAGSMIGAANLKDKH
                     RGIAQEATLVNATYFDIISYDNQFYNDFNVRVTNNSYAVLDKNIDCNSMGEYNIFSEE
                     ADQSTYEYQDLLHVWASGNSGTLACGDHIAGYGTVLSGPQCNKNGITVGAIWRLEGTP
                     LADFSSKGPTRDGRIKPEIVAQGTNVTS"
BASE COUNT         1344 a         1238 c         1207 g         1738 t
ORIGIN      
        1 agatatcaaa aagttttttc cctatgccga actggcaaca gtcgccggtg ccggacattg
       61 ggtacatgct gaaaggccgg atgccatcct tacattgata gaacaattcg ttgcagaatt
      121 ataatcctat ttcaaagatc cttaattcct gtaatacttt tcgaaccaga tatcgtaaag
      181 ttcagcggac cttaaccgca gcaattccct gtaattggca ttgctgatgg catacatatc
      241 gtatgggaag cgtgccgaaa tgaagtcccc ctttttattg agtgcctgat tgtaatattc
      301 catcgctttg atcttgttgt caagtgtcct cactaacaag acctgcctgt cctgattgcc
      361 atccttaagc gtgatcgtgg ctatcttgag gttggcgctg ctgaaatatc gcgtattata
      421 atcggttacc tgggctctaa cctcttctgt tccggtaatt tccggcttga aaatgatcag
      481 gatgaagtgt tgatctgcat cggtagcctt gtatatcttt tcggcttcgg ccagttgttg
      541 ctcgttggca tttgagccgg tacccaggag tctgagcatt tccttggctc ttttctcttc
      601 catggtattc ggataggctt gcaccaaatc cttcaatgcg gagatatagg tttctttccc
      661 tttcaggtat cccagagaca tggccgtgac cattgcaaac ttaggtcgaa ggatgttttt
      721 gatgccgaag atgctgtcgc ttttttgtgc catattgaaa gctgtggcat aatctccttt
      781 ttccatggag gtgtacgtgg aaaggtagta gtcattgagc tctgcctcct tatcggcttt
      841 atttttaata tactgcgggt cggagaaata aagcgaatat tgattcccgt tgtatttggt
      901 cttcagcagg tcggcatagt aatttgcttt ttgagagttg cccttcatca tataagcaaa
      961 gtacaactga tagaaagcct gtggttcatg tgggttgtcc ggaaaccgct gtagcaggtc
     1021 ttccagcacc tctattgctt tatcatagcg ctcgatttta tccggataaa gcacgcccag
     1081 ggcattcatg gcatccataa ttgctgcttc ggaacgagca atcgttgcag catcggtcgg
     1141 cacgtcagca agtaccttct ttatctctga cttggaaatg aataaggcag agagaccttc
     1201 ttccttatcg gtgcttgtgt tgttgtcaaa actctgaagt aatgccgaat acctccagtt
     1261 gtcggccaga ggtctgttgt tccagtcttt gtcaaactgt cttttgccgt cctttagcgt
     1321 cttatcatta taggcaaaga aggctgtcgg cttggcgcca ccgatttggc ccggattgcc
     1381 gaatgcaggg ccgcctccaa tagcagccgg ctgagagccg cctctgccta ctttagggtt
     1441 gaatgtcgag cctgtattgg atgcggtggc aagcttttcc ttttcacgtg cttccttgat
     1501 gagtttcttg gcaagagcct cctgttcttc cggtgtcatc ctgcccacca tcaacaggct
     1561 gtcctgatac tggattgttt caatattata ggctatatcc ttcaggttgt ccctcctgtc
     1621 aacgatctcc ttgaatcgtt cctttgtttt cggataggca gttgctgtgg agtcataata
     1681 atgatatgct aaatcaaatt gatcccgttt atagaaaata tcggccagct tgaggtagct
     1741 ctcggccttg gcctccggac ctttcttgct gacctggata cctttctgca ggttttttat
     1801 tccggctgcg atatcgttat tgcgtagatt gatgtctgcc agcatgaaat aaatctgatc
     1861 ttggtattcg tcgtttttaa ggtctttcaa catcttattc agcctggaat tgagttcttc
     1921 atcagatatc tttcccgtag cgaccgaaca cccaaatttg ctcaaagaag cattgaatgc
     1981 catttcgtag gagggcttaa gccgctcgac ccgctcaaaa gcctgagtag ccatatcata
     2041 attgcctgcc tttatataca actgtcccag gatatacgtc agtcgtgccc ggcgtttttt
     2101 ctctttgacc atcgggatgg ccttgttcag attttctata gcctgcgtat aactttcctt
     2161 tttcatatcc acctgggcag aaaccaacaa tgcttcccgc cgtactaagt catacgtacc
     2221 gccttcattg agcagcgacc ggaggattct ctctccatcc tcataattac ttcgttgaat
     2281 gtaggtcttc gccaaccaaa gctgtgcctg ttggaatatc gggcggtgtt tgaaaaggta
     2341 gtttttaggt ctgatggaag gcttgtcctt tttagttgct accacattgt cttcatcttc
     2401 cttaacggac tttaccggtt gtgtatctgc tttttccgtc ttcacttccg tcttgttgtc
     2461 agtcggcttg ctatccggag ttgttgtgcc tgcgggcttt gattccggtg ctttggtggt
     2521 tttcggggcg gtttttgggg tggatttgcc cttcttctta gctgctgctg cttttttcct
     2581 ttccttggct ttcttttggg ccgctttttt tctttctttg gctgccttct tacggtccgc
     2641 cgccttttgc ttggcttcct tctcctttgc ttttttagca tcggcctttt cttcccgaac
     2701 tttctccttt tcggcttcct tctctttttt tacttcctgc ctctcctttt caatggcttt
     2761 tttcttcttg cccggcgaca ttctgttggc tttgtcacga gcaagctgtg cttcactata
     2821 attctgaaca acatatttaa aggcgttttc tgctctttca taatcctttt tcaggaacag
     2881 ggcctttccc atctgaaaat aactgtcgtc cacccaatga ctggcaggat gaaggtaaat
     2941 atctttcttg agtttctcaa tggcagcatt cagggttcct gaagcggttg tggagtcctg
     3001 atccagcaaa tacgggtaga taatcaattg ttcctgaaaa ttgtccggat gcttgctttc
     3061 gatttccagc atggccgcat ccaacaattc attggcattg aaatacccgt tgtagtatgc
     3121 agtcatgttc tgataggcac gacccaacaa gccgacatct tcctttttct tatgtggaga
     3181 ggcacaactg ttgaatatca gcgctatgac gagtaaatac aggagtgccg attgatgttt
     3241 cttcaatgta tatgttcctt ttgttataat gttttataag tcagtagact taataacgtt
     3301 atttgtctga aaattcgcac aaagttacat caaaattttt acaaattgga cagattggcg
     3361 gatatgagtt gtcgccaaac aggtaaaaaa gacacctgca ttgttatcca taggatgatg
     3421 tctatcctgt aatatccttc aaaacccgac ggaggtttct tacttaacct cctcaaagtg
     3481 aatttcttcg tccttatatg ccgaagtcct ctgctgacgg ggattacaat atgccgcgga
     3541 gcaacatccc acattcaaca atggcatcaa ggtaaatata gctccaaaaa tcaggaaaaa
     3601 cacctctcca cttcgaatac cttcgaccag gataatggct ccgagaacaa gtcgcaatat
     3661 gcgtaaaaaa ttccagttgt tatgcatggc ataatgttat atggataccg gggatattat
     3721 tatcaatcgg taattatcca tatataacgg atattatgcc gattggttta atggctgatc
     3781 ctgcctattt ctgctccttt gccttacacg tgttgattcc gaaaatctgg taaatgggac
     3841 aaaaacttat ataggatgtc aatgcgaata caatggcaaa gaaaagtagc agcaaggcca
     3901 tattgcctga aatagcattt gttagatata aagcaaggat caaaactgcc aatagcaagc
     3961 gaataaattt atcgccggtt cctaaatttt ttttcatgtt ttctatttca ttaaatgatt
     4021 gataaaatta aatcattttt tcaaatgaca agtcatattg tggcaacaaa tggaaaatat
     4081 tgtcttgcag cacgaatcat tcatgccgcc aaatgagtat ttgatgggat tgggtccccg
     4141 atacccgccc tttcttaatc cttgaatatt gctaaatgac atggtcaaag gtgtacttta
     4201 tttcatattt ttatactaag tttactgaaa aatacttctt atttcatgtt attaaaaata
     4261 ctccaaatga acctcatcat cagatttttt ttcggtatag ccatgattgc tcttgctcac
     4321 atggtcaatg gccaatctgt caaaccgata cataccgtcc atggcagttt taccccacct
     4381 cctaaagagg ttgtccttca aaatcatgaa agccgcaacc cttcgcgtga aaaaattgta
     4441 tggatacaac ttgcggatga taccataaat ggttttcggg attcgattgt caatcggttg
     4501 agtcctgtgg attttatcga tgacaggacg tttgctgtga gggtcgatgc agtcggattg
     4561 aaagatcttg ctgcaaatga ggctgtagtg tcgatctttg aaatgccggc agcattaaaa
     4621 ctggaccgta aggtggtgga tgcggatgac tccgaaaaac tggaggttag gatcagctta
     4681 tatggagcta tgtctgattc agagattgaa aatctgatcg gaccggtaaa aataatctct
     4741 catcatgttt ccggtgacaa aacttacttg gtgattgcct gtgaccggcc atctgccttg
     4801 gagatagctg ccggtgctca agtcagagcc atctccgaaa atatatctgc atttacaccc
     4861 ctcaacttct acaagcgctc actggaagca gtacaaggag caaatgcatc cttgcaaaat
     4921 ggtggattgg gactaaatgg cgccggtatc acaatcggtc atggtgacac aggggggttt
     4981 ttccatgaag acctgaatga cagggtagtc catttcgctc tggattcagt gaccgcccat
     5041 gctcctcata ctgcaggaag catgatcggg gcggctaacc tcaaggataa acaccgtggc
     5101 atagcacagg aagcaacact ggtcaatgcc acttattttg atatcatctc gtacgacaat
     5161 caattttaca atgacttcaa tgtccgcgta accaataatt catatgcggt gctggataag
     5221 aatatcgatt gcaattcaat gggcgaatac aatatattca gtgaagaggc ggatcaaagt
     5281 acctatgagt atcaggacct tttgcacgtg tgggcgtcgg gcaacagtgg cacgcttgcc
     5341 tgtggtgacc atatcgccgg ttatggaact gtcctttcag ggccgcaatg caacaagaac
     5401 ggtatcaccg taggcgccat ctggaggctg gagggtacac ctcttgccga tttttcaagc
     5461 aagggtccga caagagacgg cagaatcaaa ccggaaattg tggcccaggg gactaatgtt
     5521 accagct
//
LOCUS       DAOQQN010000217         5482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6621_length_5482_cov_6.637737, whole genome shotgun sequence.
ACCESSION   DAOQQN010000217 DAOQQN010000000
VERSION     DAOQQN010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6621_length_5482_cov_6.637737"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1083
                     /locus_tag="PLG24_08680"
     CDS             115..1083
                     /locus_tag="PLG24_08680"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HMZ25007.1"
                     /translation="MKSIIGCIVFALCLASHVAKGQLFVPADTFRQGRAIAITGTGLA
                     VYSGGMYLLGKAWYKDNLTNSFKFFNDNGEWLQMDKVGHVFSAYNEAGFVHDLYRSAG
                     VRERKALWLGIGAGLLAQTSIEIFDGFSDRWGFSWGDVAGNFAGLALFGFQQAAWHEQ
                     KLKLKVSSDFRSYSTSDILPLSGSESDNLHRRAGELFSNNLAGRLLKDYNAQTYWLSF
                     SPADFSSQRFWPAWLDISIGYSGENLFGGYGNTWDYQGNTYQLSGRDFPRYRQFLLSL
                     DLDLTRIPCRSAFLRDVFRVLNIIKIPAPTLELNSLGKLKGHWIYF"
     gene            1159..1608
                     /locus_tag="PLG24_08685"
     CDS             1159..1608
                     /locus_tag="PLG24_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25008.1"
                     /translation="MKRFLRKLLLYVIGFGIGLLILANVTSYSKGERTGHIVKLEKKG
                     LLFKTWEGSLDMSIFQGARPNKGNVENTLWTFTVSDDKIAQQIQDANMRGNRVILHYK
                     EKYMKLFWIGDTKYIVIAVDEVKENPPQSPSDNPPIMGTQPGTGEKL"
     gene            complement(1627..2721)
                     /locus_tag="PLG24_08690"
     CDS             complement(1627..2721)
                     /locus_tag="PLG24_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25009.1"
                     /translation="MKNLFTLNYIGQALLAMLLIQQVDAQVTQFYKRPVSDSTLSEGF
                     SLHGSSLLYFRNNEYFSGAYPGFTALGANNELSGNYRHGRHFISTGMMYSLAFGQPMR
                     HRVLPVITYQYSLARHLNIVMGTLSGPAEHKLPQELLGLDRYLYHPLEYGLQFTYQPG
                     WMDADIWIDWRNNIMHGDSAQEEFTQGSRFIFHLLNKGGWNIDANAILLFYHNGGQID
                     RSDAPTMTRINYSVGMNLSRQISRWKYSISGQYFGFYNDEDFTAFPWKEGNAYSISLQ
                     AENATLCLGTAFKNFHHFFTLNGDQVFSIFNQYESRIYGESKKLVTGFARIQLMNIKN
                     FKIGFGAELYHDLNSRHTDYNYNIIGRFLF"
     gene            complement(2718..4475)
                     /locus_tag="PLG24_08695"
     CDS             complement(2718..4475)
                     /locus_tag="PLG24_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ25010.1"
                     /translation="MSKNIDFNLLLRVVRFSFDYKRMFYAALIMALMLAPLGALRPYL
                     INLGVDKYILNSDMSALLTLCWVIVSLVIVEAGLRFFFNYLTESLGIHVTRDLRIKIF
                     NKIVGQRVRQFDQTPTGMYITRIINDIESIKTIFSEGLLTIIADIITIIAILIMMFVT
                     SWQLTLACLVVMPLMVIATVIFNKKVKGSFQRVRAQLANMNAFLQEHLSGMTIIQAFN
                     AQKREAARFREINRNYTRANLDSVLYYAVFFPVVDIISAAVLGLMVWWGARGIMDGVV
                     TLGAMVAFPIYINTLFRPIRMLADKFNSMQQGLVAAERIFNLMDDTSVNEDSGHLQLK
                     NVEGHIQFDHVSFEYVEGNPVLHDVSFEVPRGTTLAIVGSTGSGKSTIINLLMRFYDY
                     NQGFIRLDGIELRDISKTSLRSHISLVLQDVFLFSGNVRDNITLKNPDITDEAIFKAA
                     KMLDAHRFIMALPGGYDFLVSERGYNLSSGQRQLISLVRALVYNPSILILDEATASID
                     TETEAIVQFAIDKLLRGRTAIIIAHRLSTIRNADNIMVLEHGRILEQGSHDMLLQNSG
                     SRYSELYNIQFEHKLSAGL"
     gene            complement(4877..>5482)
                     /locus_tag="PLG24_08700"
     CDS             complement(4877..>5482)
                     /locus_tag="PLG24_08700"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25011.1"
                     /translation="SRIFHAEAKNIFRTYFGGAVNLPGIGLELSLHQNIVTNHTFFDN
                     DWRMQQTAKAISVTGIQAQYNLKAGPFHLDNSVLWQASTDKDIRVPGLAGSHAAYWEA
                     SLLKRRLLINIGAEVRYLTAFDRIGYSPLLFSFYNTDAGFQSSMLTYDAFISYKIRLL
                     RGFIRADNINSLYDNSAQYQIAPYPIDAFAIRIGFDWIFNN"
BASE COUNT         1561 a         1105 c         1296 g         1520 t
ORIGIN      
        1 actggatccg gaagcaaggg ccgggttgaa ggatgatgac cttaatccga agtttaccag
       61 cgtaggagta agtctgatta tccacaacag aatgtgatga atggctgccg gcaaatgaaa
      121 tcaataatcg gatgtattgt atttgccttg tgcctggcct cccatgtcgc caagggtcaa
      181 ttgttcgttc cggcggatac cttcaggcaa ggcagggcaa ttgccatcac aggaacagga
      241 ttggctgtct atagtggagg aatgtacttg ttgggtaagg catggtacaa ggataacctg
      301 actaattcct tcaaattctt caacgataat ggcgaatggc ttcagatgga taaggtgggg
      361 catgttttca gtgcatacaa tgaggccggg tttgtgcacg atctgtaccg atcggctggc
      421 gtaagagaaa gaaaggcttt gtggttaggc ataggagcag gcttactcgc acaaacaagc
      481 attgaaattt ttgacggttt ttccgatcga tgggggtttt catggggtga tgtagccggg
      541 aattttgccg gtttggcatt gtttggtttt caacaggcag cctggcacga gcagaaattg
      601 aagcttaaag tgtcttctga tttcaggagt tattctacat ccgacatatt gccattgtcc
      661 ggttctgaga gtgacaatct acacagaagg gcaggagaac tgtttagcaa caacctggcg
      721 ggtcggttgt taaaggatta taatgctcag acctattggc tcagcttcag cccggcagat
      781 ttttcatcac aaagattttg gccggcctgg ttagatatat ccataggtta tagtggagaa
      841 aatttatttg ggggctatgg caatacctgg gattatcagg gcaatactta ccagctaagt
      901 ggccgggatt ttcctcggta tcgtcaattt cttctttcat tggatctgga cctgacgcgc
      961 atcccttgtc gttcagcatt tctcagggat gtctttcgtg tgctcaatat tatcaaaata
     1021 cctgcaccta cactggaatt aaattcattg ggaaaattaa agggtcactg gatatatttt
     1081 tgaaatagaa atgaatatat caatctgaat gaatcatttc attgctatct ttgttaattg
     1141 gattaaatat caaaacgaat gaaaagattc cttaggaaac tcctgttata tgttatcggt
     1201 tttggaatag gattattgat tttggctaat gtgacaagct atagtaaagg tgagcgtaca
     1261 ggacatatcg tcaaacttga gaagaagggc ttgttgttta agacctggga gggaagcctc
     1321 gatatgagca tttttcaggg agccagaccc aataagggca atgtagagaa tacactctgg
     1381 acttttaccg tatcggatga caagattgca caacaaatcc aggatgccaa catgagaggt
     1441 aacagggtca tcctgcatta taaggaaaag tacatgaagc tcttctggat aggtgatacc
     1501 aaatatattg ttattgcggt ggatgaggtt aaggagaacc ctcctcaatc gccttctgac
     1561 aatcctccta ttatggggac gcaacccgga accggagaga agttgtagtc taatggatat
     1621 ccattctcag aataggaatc gtcctataat attgtaatta taatccgtgt gcctggaatt
     1681 aaggtcgtgg tataattcag cgccaaaacc aatcttgaaa ttcttaatat tcatcaactg
     1741 aatacgggca aagccggtga ccaacttttt ggattctcca taaatccggg attcgtactg
     1801 attgaagatg ctgaatacct gatcgccatt gagggtgaag aaatggtgga aatttttaaa
     1861 ggcagtacca aggcataatg tagcattttc tgcctgcagt gaaatactgt acgcattgcc
     1921 ttctttccag ggaaaggcgg taaagtcttc atcattgtag aatccgaaat actgaccaga
     1981 tattgagtat ttccaccggg agatctgcct ggaaaggttc atcccaacac tatagttgat
     2041 tcgggtcata gtcggcgcat cgctacgatc gatctgaccc ccattgtgat aaaaaagaag
     2101 aatggcgttg gcatcgatgt tccatccccc tttgttcagc agatgaaaga tgaaacggct
     2161 tccctgtgtg aattcttcct gagcagaatc tccatgcatg atgttatttc gccaatcgat
     2221 ccatatatcg gcatccatcc accccggttg gtaggtaaac tgtagtccgt attcgagggg
     2281 atgataaaga tatcggtcca aaccaaggag ttcttgtggt aatttatgct ctgcagggcc
     2341 ggataaggtg cccatgacga tattcagatg cctggccaga ctatattggt aggtaatgac
     2401 aggaagtacc cggtggcgca tgggctgacc gaaagccaga ctgtacatca tgcctgtact
     2461 gatgaagtgt ctgccgtgtc gataattgcc tgacagctca ttgttagctc ccaatgctgt
     2521 aaaccctgga taggcaccgg agaaatattc gttattcctg aagtacaaca aagaagatcc
     2581 atgcaaagaa aagccttcgg aaagagtaga atcagagacc ggacgcttgt agaattgggt
     2641 cacctgcgca tcaacttgtt gaatcagaag catcgccaaa agagcctggc ctatataatt
     2701 caaagtaaaa agatttttca tagcccggca cttaatttat gctcaaattg aatattgtat
     2761 aactcactgt acctgctgcc ggagttttgc agcaacatgt catggcttcc ctgttccagt
     2821 atacgaccgt gttccagtac catgatgtta tcagcgttac gtatggtgga caagcgatgg
     2881 gcgatgataa tggctgtcct accccgcagc aacttatcaa tggcaaattg gacgatggct
     2941 tccgtttcgg tatcgatgct ggctgtggcc tcatccagga ttaggatgga agggttgtac
     3001 accagggcgc gaaccagaga tataagttgt ctttggccgg agctgagatt atatccgcgt
     3061 tcggaaacca ggaaatcata accaccgggc aaggccatta tgaatctgtg ggcatcgagc
     3121 attttagcag ccttgaagat tgcttcatct gtgatatccg gatttttcag ggttatattg
     3181 tcccggacat tgcctgagaa aagaaagaca tcctgcaata caaggctgat gtgcgaacgg
     3241 aggctggtct tacttatatc gcgcagttcg ataccgtcca gacggatgaa tccctgattg
     3301 taatcgtaaa aacgcatcag caggttgata atggttgatt tgccgcttcc ggtacttcca
     3361 acgatggcta aggtagtacc tcttggaacc tcaaaggaaa catcgtgcag gacaggattg
     3421 ccttcgacat attcgaagct tacatggtcg aattgaatgt gcccttcgac atttttcagt
     3481 tgcaagtgac cggagtcttc attgacagat gtatcatcca tcaggttaaa gatgcgttct
     3541 gcagccacca agccttgctg catgctgttg aacttgtcgg ccagcatcct gatcggcctg
     3601 aacaatgtat tgatataaat ggggaaggcc accatggctc ccagggtcac cactccatcc
     3661 atgattcccc gtgctcccca ccagaccatg aggcccagca cggcagcaga aatgatatct
     3721 acaacaggaa aaaatactgc atagtagagt acagaatcaa ggttggcacg ggtataattt
     3781 cggttgattt ccctgaacct ggcagcttct cttttctgtg cgttgaaagc ctgaatgatg
     3841 gtcattccgg acagatgttc ctgaagaaag gcgttcatat tggccaattg ggcacggaca
     3901 cgctgaaacg aacctttgac ctttttgttg aagataaccg tggcaatgac catcagcggc
     3961 atcactacca gacaggcaag agtcagttgc cagctggtca caaacatcat aatcaggata
     4021 gcaatgatgg taatgatatc agcaatgatg gtgagcaaac cttcagaaaa aatcgtcttg
     4081 atactttcaa tatcattgat gatgcgggtg atgtacatac cggttggagt ctggtcaaac
     4141 tgtctgactc gctgacctac aattttattg aatattttga tacgcaggtc tcgggtaaca
     4201 tgaattccga gagattcagt gagataatta aagaaaaaac gaaggcctgc ttccacaatg
     4261 accaggctta caatgaccca acagagtgtc aataatgcgg acatatcgct atttagtatg
     4321 tatttgtcca ccccgagatt gatcaaatag ggtcgcaatg cccctaatgg agccagcatg
     4381 agagccatta tcaaggcagc atagaacatc cttttgtagt cgaaggaaaa tcttactacc
     4441 cttaaaagta aattaaagtc aatgtttttg gacatatagc acaacgggaa tcatatcgac
     4501 tgacaatatg atttcattgt aaaattagga ataaattaat aattggacac tgcagaacga
     4561 attatggtgt gtttagcatc tttcatacta atttcattat gaagaaaagt tatgctgcta
     4621 tttaatagta agatcattgc aaaaagtgac aacaagcaga taccatttat ttgtagtagc
     4681 ttctgttcaa cccttcaaat gacacatttc ataaggctga acctgcggag attttattta
     4741 tagctaaatc cggaaattta atgagtgcaa tttgttattc gtacaaagaa cttttatcaa
     4801 agtttgttta gaaggaatag cagccgcatc ctttttccgg cattcggttt aatactgtta
     4861 tagaagcaag tcggtattaa ttgttaaata tccagtcaaa tccgattcgg atagcaaatg
     4921 catcgattgg atagggggca atctggtatt gggcgctgtt gtcatacagg ctgttgatgt
     4981 tgtcagctct gataaaacca cgcaacaacc ttattttgta agatataaag gcgtcgtagg
     5041 taagcatgga ggattggaat ccggcatcag tattgtagaa gctgaaaagc aaagggctgt
     5101 agcctattcg atcaaaagct gtgagatatc tgacttcagc accaatatta attaatagcc
     5161 ttcttttcaa tagtgatgcc tcccaatagg cggcatgact tcctgccagg cccggaactc
     5221 ggatgtcctt gtcggtactt gcctgccaca agacactgtt gtccagatgg aatgggcctg
     5281 ccttcagatt atattgagcc tgtattccgg ttacggagat agccttcgct gtttgctgca
     5341 ttctccaatc attgtcaaaa aaggtatggt tggtaacgat gttctgatgc aaggataatt
     5401 ccaggcctat gcccggcagg ttaacagctc caccgaaata ggttcggaaa atgttttttg
     5461 cctcggcatg gaatattctt ga
//
LOCUS       DAOQQN010000218         5462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6661_length_5462_cov_6.096357, whole genome shotgun sequence.
ACCESSION   DAOQQN010000218 DAOQQN010000000
VERSION     DAOQQN010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6661_length_5462_cov_6.096357"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PLG24_08705"
     CDS             <1..441
                     /locus_tag="PLG24_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25012.1"
                     /translation="DMKILARQPLTIACAYLWIGFVGAISLMETWINTKINGSTPTNE
                     LGRIVIIYLNKIEWVFAISIILNIVFYRNRTGLYSYILLFLTMLILTGQTFWLIPELR
                     DNLLDHVHGHEIPPNYLHIFYLGGDAIKVICLFLLSFTMYRQID"
     gene            602..1192
                     /locus_tag="PLG24_08710"
     CDS             602..1192
                     /locus_tag="PLG24_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ25013.1"
                     /translation="MLIDYDILLTYGGLARKYKKGAVIFHEDDTPHFYFQVLEGSVTV
                     LSTNSEGKDLIQGIYSPGDGFGEPPLLANKPYITTAIANCDSVIIKVGKENFLNILHD
                     FPEITLRILNYLGERLYQKYYTSRLLTSHTPEEKISYFLNNLKPGPAKEPIAKVPYTR
                     QQIADFTGLRVETVIRTLIKMKKEGKVKIINHKLYY"
     gene            1198..2406
                     /locus_tag="PLG24_08715"
     CDS             1198..2406
                     /locus_tag="PLG24_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25014.1"
                     /translation="MNNSIPTKYFRYSVISLFIVAVLGTLMRYKIAFSFPYFDQKNLL
                     HAHSYFAFSGWISHFLYCGLASIILKYITTKRYNILITANLVCATGMLVSFAIKGYNI
                     TSIIFSTCGILVGLLFAIQLIGDWRKVKEEHPSKNWSIAAVALNIIASAGPLFLAYMI
                     FSHTTTHKTYLGSVYYYLHFQYSGWFFFASMAMIVDRIQNKTSILNKYFLVFAITVIP
                     TFFLSILWANLPMWLYAITVAATFVQLIAWYHLLYKYFKDVNHYLKENFPSWVRLLCY
                     SAILAISIKFLLQAISVIPSLSQLVFGIRPIVIAYLHLVLLGVYTLFFLAYLVMNNYL
                     KQKPIIKKATMAFLTGVILNELLLGIQGFAAFFYIPIPYVNLLLLLAALVLLISAGTL
                     MASQFSTGED"
     gene            2676..2822
                     /locus_tag="PLG24_08720"
     CDS             2676..2822
                     /locus_tag="PLG24_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25015.1"
                     /translation="MGAQRKTWSKEEKLSAISMAKTESFANASKRYNVSVTTLYYYSD
                     KKNE"
     gene            3386..3712
                     /locus_tag="PLG24_08725"
     CDS             3386..3712
                     /locus_tag="PLG24_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25016.1"
                     /translation="MWEKLKNLSEILFRPLVIGYGLYFLYILYNCFKGNKYVEDKSIL
                     IQYLILKLLPALLILGVAFILKAKGNTKLAGLVLGVPALYVIVASLFFPVLWYVLSVV
                     RIIFGK"
     gene            3843..4358
                     /locus_tag="PLG24_08730"
     CDS             3843..4358
                     /locus_tag="PLG24_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25017.1"
                     /translation="MNYSGEMIYSLPDNGKGIYSELPVKYTLNCPMEIFTNTDIKSGP
                     CRITSVSNDLADTPDPFYADPMPKGMGKILYDENSPIPKNVITLNLAQNVKPDKVKIF
                     NSVNLCTMELEIKGTDKYVMDFSTYDPGFYSVSFFLFEKLIYSFTLIKCYPLVVLQDK
                     ANKLITTPTIW"
     gene            4398..5195
                     /locus_tag="PLG24_08735"
     CDS             4398..5195
                     /locus_tag="PLG24_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25018.1"
                     /translation="MGQGVNQFLGYLEITSITNFSLKNNEIPEDCKFTLGNFKFVQYP
                     DCQQLILWLPDHGDNYDSITLYRLKPEKEVWKKKISDILSGSIKIILDTSEIEPGKYK
                     IVIIKYADLKHEISFNKSADYFKPPEDMVTILNDELEKPIVYSDGSGNKIPDEDLIMR
                     ETLYNKTVEKLMRKVRYENFGRGGYAIFVEGSRSIKFEMEYGGGNCSLILFIPSIDNW
                     EALTGFSIGEREEIIMFVAERVLGDQFVSSNVIYKISDSDIMYLKNQ"
BASE COUNT         1823 a          986 c          957 g         1696 t
ORIGIN      
        1 gatatgaaga ttcttgccag gcaacctctg acgatagcat gcgcttatct ctggattggc
       61 tttgtcggcg caatcagtct catggagacc tggatcaata ccaaaatcaa cggttctact
      121 ccgacaaatg aattggggcg gattgttata atttacttaa ataaaattga atgggtattt
      181 gccatttcaa ttatactgaa catcgttttc tacagaaaca ggaccggcct atactcctat
      241 attttacttt ttttaacaat gctaatattg acagggcaaa cattctggtt gattcccgaa
      301 ctgagagaca accttttgga ccatgtccat gggcatgaaa tcccccccaa ctacttacac
      361 atcttctatc tgggaggtga tgcaatcaag gtaatatgcc tgtttttact ttcctttacc
      421 atgtacaggc aaattgatta ggcccattaa atttaaacca aaccacaaca aacaaaaata
      481 atattataac ttttccctta aattcaaaga aaacaataaa attttcaaaa aactacaaaa
      541 cttccaagaa tactgtattt ttatggcaat aacacgtcgc cctattaatt aatggagtgt
      601 tttgctgatt gattacgaca tattgttgac ctatggcgga ttggccagga agtataaaaa
      661 aggcgctgtc attttccacg aagatgatac acctcacttt tactttcagg ttttggaagg
      721 gagtgtaaca gtactatcga ccaattcgga aggaaaagac cttatacagg gcatctattc
      781 tccgggtgac gggtttgggg aacctccact tctggccaac aaaccatata ttaccaccgc
      841 aatagccaat tgcgatagtg tcatcataaa ggtaggtaaa gaaaattttc taaacatcct
      901 gcatgacttt cctgagatta cattgcgtat ccttaattat ctcggcgaaa ggctttatca
      961 aaaatattac acatccagat tactgaccag ccacactccg gaagaaaaaa tcagttactt
     1021 tctcaacaat ttaaaacccg gaccggccaa agaacctatc gcaaaagttc cctatacgag
     1081 acagcaaatt gccgacttta caggcttgag ggtagaaaca gtcataagga cactcatcaa
     1141 gatgaagaag gagggcaagg tcaaaataat taatcacaaa ttatactatt gagaaccatg
     1201 aacaactcaa tcccgacaaa atattttagg tattcagtta tcagtctgtt tattgtcgct
     1261 gtacttggca cactgatgcg atacaagatt gccttcagct ttccttattt tgaccaaaaa
     1321 aacctccttc atgcccattc ctatttcgca ttttcaggat ggatctccca ctttttatat
     1381 tgcggattgg catccatcat cctaaagtat atcacgacca agaggtataa tatcctgata
     1441 acagccaatc tcgtatgtgc caccggcatg ttggtttcat tcgcaatcaa aggttataat
     1501 atcacatcca ttatcttctc cacttgtggt attctggttg ggttattatt tgccatccaa
     1561 ttgattggtg actggagaaa agtcaaggaa gagcatccgt caaaaaactg gtcgattgct
     1621 gcagtcgccc tcaatatcat cgcttcagca ggaccattat tcctggctta tatgatattt
     1681 agccatacaa ccacacacaa aacttatctt ggatcagtat attactacct gcattttcaa
     1741 tacagcggct ggtttttctt tgcatcaatg gccatgattg ttgaccgaat ccagaataag
     1801 acttccatcc tcaataaata cttccttgta tttgccatta cagttatccc tactttcttc
     1861 ctttcaattt tgtgggccaa tcttccgatg tggctttatg ctataacggt agcagccacc
     1921 tttgtccagt taatcgcctg gtaccatctt ttatacaaat attttaaaga tgtaaatcat
     1981 tatcttaagg agaatttccc ttcctgggta aggttattat gttattcagc gattctggcc
     2041 atcagcataa aatttttact acaggcaatt tccgtcatac cgtcattaag tcaactggta
     2101 tttggaatcc ggcctattgt catcgcctat ctgcacttgg tattattggg agtctatact
     2161 ctttttttcc tggcatattt agtaatgaac aattatctaa agcagaagcc aatcataaaa
     2221 aaagctacaa tggccttcct tacaggtgtt atattaaatg aattgctact cggaatacaa
     2281 ggctttgccg ctttttttta cattcccata ccatatgtca acttgttgtt gttacttgct
     2341 gcattggtgt tactaatcag cgcgggcact ttgatggcct cccagttcag taccggagaa
     2401 gattgaattc caaactttgt atgcaaattg aaactaatgg tgctgataat tggatataaa
     2461 cgcaaaaaag gatatactaa agtgtctgac ttaccacata cgactataca atcagttgaa
     2521 taaccataca agtttaaaat attccgttag tgtacacatg caatagtgta aataagcctt
     2581 caaagcaagt tttataattc atagaaaact ttctaacccc ttaaaaaacc ggacacaaat
     2641 taaaattaaa tagtttaaaa aaaataaatt tgtgtatggg tgctcagaga aaaacatgga
     2701 gtaaagaaga aaagctgtca gccataagta tggccaaaac agagagcttt gccaatgcgt
     2761 caaaaaggta caatgtttca gttacaacac tatattatta ttcagataag aaaaatgaat
     2821 agaatttatt tacagaaggc aatccggaag aaatgtttcc aaataacagg atgggaaaaa
     2881 ataatgaaaa tcttataaaa caaataacat acatctctaa agatggagat actctttcat
     2941 tgattaatta taaatattga gataccaata gtgaaaacca attaacattc ttgcttaccg
     3001 atgaacttaa tcagaatgaa acaatcataa cctatcattt tcaaaacaat tgagatgcaa
     3061 ccttctacat tttttacgag tccactatta aacaagcttt attggccact tatggcaata
     3121 tatataatta ttctgttagt cctcattatg ctgtatttta ctcaaataaa caactgggcg
     3181 gatcggggtc attataatat gatgaatttt agaagaatcg gtttcgtagc catattaatt
     3241 ggcgcctcct tgtatatgaa gttttgggga aatattaagg cagcaaacat gattctttat
     3301 ataccgacat tcatcttgct tttgattttg gtgcttggta taataattat gtatatattt
     3361 tcaacatcaa taacaccaaa atagcatgtg ggagaaatta aaaaacctaa gcgaaatttt
     3421 attccggcct ttggtaatag gatatggttt gtatttttta tatatccttt ataattgttt
     3481 caaaggaaat aagtatgttg aagataaatc aatcttaata caatatttaa ttttaaaatt
     3541 attgccagcc ttgttaattc tcggagttgc ttttatctta aaagcaaagg gcaatactaa
     3601 attagccgga ttggttttag gagtgcctgc actatatgtg atagttgcct ccctgttttt
     3661 tccggtatta tggtatgttc tttctgttgt caggataatt ttcggtaaat aaaactaata
     3721 attcattgcc cctaatagca cttaaagagc cctttatcag gcattatgca ggaagtgtat
     3781 tttttcttcc gcaaccgatt tttagctcta aatgggatga agatgcaggg attacggacc
     3841 gaatgaatta cagtggtgaa atgatttatt cattaccgga taatggtaag ggaatttatt
     3901 ctgagttgcc ggtaaaatat accctaaatt gcccaatgga aatatttaca aatacagata
     3961 tcaaatcagg accatgcagg attacatctg ttagtaatga tttagcagat acacctgatc
     4021 ctttttatgc tgacccaatg cctaaaggta tgggcaaaat tttgtatgat gaaaattctc
     4081 caatacccaa aaatgttata actctgaatc tcgcccaaaa tgtcaaacca gataaagtga
     4141 aaatcttcaa ctctgtaaat ctttgtacaa tggaattgga aattaaagga acagataaat
     4201 atgtgatgga tttttcaact tatgatccag ggttttatag tgtttcattt tttctgtttg
     4261 aaaaattaat ctatagtttt accctgatta agtgttatcc attggtggtt ttacaggata
     4321 aagcaaataa attgataaca acaccaacga tctggtaaaa agtatgtgca atattttatc
     4381 taaaataaaa actaatcatg ggtcaaggtg tcaatcagtt tttaggatac cttgaaataa
     4441 caagtattac taattttagt ttaaaaaata atgaaatccc ggaagattgc aaatttacgc
     4501 tcgggaattt caaatttgta caatatccgg actgtcagca attgattcta tggcttcctg
     4561 accatggtga caattacgat tcgatcacat tatacagact aaaaccagaa aaagaagtat
     4621 ggaaaaagaa aatcagcgat atactaagtg gaagcattaa gataatttta gatacttccg
     4681 aaatcgaacc aggtaaatat aaaattgtaa tcataaaata cgcagatttg aaacatgaaa
     4741 tcagttttaa taaatctgct gattatttca agccgccgga agacatggtc acgattttaa
     4801 atgatgagtt agaaaagccg attgtgtata gtgatggatc aggaaataaa atcccggatg
     4861 aagatttaat tatgcgagag acattgtaca ataaaactgt cgaaaagcta atgagaaagg
     4921 taagatatga gaattttgga agaggtgggt atgcaatttt tgtggaagga agcagaagta
     4981 ttaaatttga gatggaatat ggaggcggaa attgcagttt aattcttttc ataccatcaa
     5041 tagataattg ggaagcttta accggttttt ctattggtga aagagaagag attataatgt
     5101 ttgtggcaga aagggtattg ggagatcagt tcgtttcatc aaatgttatt tataaaattt
     5161 cagattcaga tataatgtac ctcaaaaatc aatgagcaga ttaaagcaaa atgtggggac
     5221 agtaaacaat taatactgtt agtccgtgat gaatcagccg gcgtagcctt gcctactcga
     5281 ccatctcagg caaaaacacc tgaggataat tctccggcca taatctttct tgcgatgatg
     5341 ctgcatgcct cttccaacag atcaaacacc tgtctgtaac catcatatcc accataccag
     5401 ggatcgggaa cacttgcatt ggatcccgga ttgacctcat tcagcaaata gagaaccttc
     5461 tc
//
LOCUS       DAOQQN010000219         5436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6705_length_5436_cov_6.539119, whole genome shotgun sequence.
ACCESSION   DAOQQN010000219 DAOQQN010000000
VERSION     DAOQQN010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6705_length_5436_cov_6.539119"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLG24_08740"
     CDS             complement(<1..275)
                     /locus_tag="PLG24_08740"
                     /inference="COORDINATES: protein motif:HMM:NF025028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY domain-containing protein"
                     /protein_id="HMZ25019.1"
                     /translation="MKFLLLLLIFLIFIVIFAGCETGYVKKNGTWVWVTIDENYGKRN
                     HAIVGVDQGTFKVLKNKNYASDHMNVFFRGKKVNYADPKTFECLTFD"
     gene            complement(289..1077)
                     /locus_tag="PLG24_08745"
     CDS             complement(289..1077)
                     /locus_tag="PLG24_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25020.1"
                     /translation="MDDNTKYPGQIEIDRVDNISAGPIGTEGLNNFSLEHVSTTHYSD
                     CQQIIVYLPVSYVHYLSVSIINIENGEMIFVDDVNNLVLGSVKLILASDWIHPGKYRM
                     EILSRENIQHHICFTKRPDMLSSTDSGPLSEEKKEDQTPMEYRDGFGRTIKNSDLDMR
                     KNAIEQTLGKIFRSVEFVNQGKEGTVIYHEFSRTIKFYMELGGNDIVFYLNIPSASDW
                     EHVTGFSLQERDSIIEFVANETQKHQAPNCTYSIEEATIYFIHK"
     gene            1493..3100
                     /locus_tag="PLG24_08750"
     CDS             1493..3100
                     /locus_tag="PLG24_08750"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HMZ25021.1"
                     /translation="MLRYLFLLCCFFSYDASARIFYIDPVNGSMDNDGSYDYPWRTLE
                     EVVAADLIQSYSYTPLPYDPNSSTLIIKNENGPVEPGDTLMLRSGMHGEFLLRGYINP
                     KMITIMADEGHKPILKQIRLQACKNWRFENLIISTEPYGYYLNDRLVFLETHGWHGPT
                     SGISILNCEIYSGEQPWETAEEWLANASSGIIIEGDSMIIENNYLKNVVMGITASGDH
                     IHAINNVIENFSADGMRMQGNDILFEGNTIMNCYDVDENHDDGIQSFIIGEGVFRNNI
                     IRSNIILNYVDQDQKLLGALQGIGCFDGFYENWLIENNVVIVNHWHGITLLGARNCTI
                     RNNTVLDPTPDIRPGGSWIMIDSHKDGRPSEGCIVANNVANSINANAQVNNNVVMDTY
                     EAYGSNFMDYKSFDFHLKEGSILIDSGSLQNAPETDIDGVVRPQGGGVDIGAYEWIVM
                     TSERHTIQNDIRIFPNPSTGHIHVEHLEGSGVVEVYDMRGHRLHQEKYTSQSLRLNLQ
                     DLKPGVYWIKIRSERIGTFHACRFIKL"
     gene            3654..4517
                     /locus_tag="PLG24_08755"
     CDS             3654..4517
                     /locus_tag="PLG24_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25022.1"
                     /translation="MKVKQIDRVELKRRGFISSFSGSSGIIYSDGFVYIIADKSNVLS
                     VFDVNDQTLKKEISLKADGSLEANVEKKYKPDFEAFSRHGNRYFIFGSGSAKNRFDLV
                     ILDERFDFVERLSIKNLYLAMMESTGIDRDNFNIEGVILEEDFALFFNRGNGPDRKNG
                     IIKVRNWLTDEPEVAGYSSIVLPDINGRHAAFSDAILHDGCICFLANGEDTQSVYDDG
                     AVAGSAFGMMDLSTFELLEFHIIDHSMKLEGLAVYKDLKEEHKMVFIVCDDNDFEHQT
                     IISQLTVFWRD"
     gene            4924..>5436
                     /locus_tag="PLG24_08760"
     CDS             4924..>5436
                     /locus_tag="PLG24_08760"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HMZ25023.1"
                     /translation="MRNRSIACIFALFLGGIGVHKFYLGKVGQGVFYAIFSMTFIPVF
                     LGFIDFIRLATMSEDEFNRLYNDYIPFTTPKEWQTKHRYRNAKQDTVVFENRRNEPGR
                     DKDSFSQKERRKKRPMPLTANRAYKDGVKLLEDYRYREALEQFERARKASGDHPEILF
                     RMACCNAVLEQ"
BASE COUNT         1635 a         1025 c         1165 g         1611 t
ORIGIN      
        1 tcatcaaaag tcaggcattc aaaggttttt ggatcggcat aattaacttt tttcccccta
       61 aaaaatacat tcatgtggtc agatgcatag tttttatttt tcaacacctt aaaggtaccc
      121 tgatccacac caacaatagc atggtttcgt ttaccatagt tctcatcaat ggtaacccaa
      181 acccaagtgc catttttttt gacatatccc gtttcacaac cagcgaaaat gactatgaaa
      241 atcaggaaga ttaacagcaa taacaggaat ttcattttgt cttaagtttc atttgtgaat
      301 aaaatatatt gtagcttcct ctatagaata ggtgcaattg ggggcctggt gtttttgcgt
      361 ttcatttgct acaaattcaa taatagaatc tctttcttgt aatgaaaaac ccgtgacatg
      421 ttcccagtca gatgcagagg gaatatttaa gtaaaagaca atatcatttc ctcccaactc
      481 catataaaat ttaattgttc gggaaaattc atgataaatg accgtacctt ccttaccttg
      541 attgacaaac tctacgctcc ggaatatttt tccaagggtt tgctctattg cattctttct
      601 catatccaga tcagaatttt taattgttct gccaaatcca tcgcgatact ccatgggtgt
      661 ttgatcctct tttttttctt cagacaaagg tccggaatca gtggaagata acatgtccgg
      721 tctttttgta aaacaaatgt gatgttgaat attttcccga gacaatattt ccatcctgta
      781 tttgccggga tgaatccaat cacttgcaag gattaatttc acactaccca atacaagatt
      841 attcacatca tcaacaaata tcatctcgcc attttcaata tttattatgc tgaccgataa
      901 ataatgtaca tatgatacag gaagataaac aataatttgt tgacaatcgg agtaatgggt
      961 agttgaaaca tgttcaaggg aaaaattatt caaaccctcc gtccctattg gtccggcgct
     1021 aatattatca acccggtcaa tttcgatctg acccggatat ttagtattat catccataat
     1081 acaccaacag caaccttgta caaaaatagt gataatcccg aacctgaatt ataatccgga
     1141 tatctctgat gacacttttt cagctatatt tagcggtagt ggtgtgtggc tcctaaaaag
     1201 tattggcaga tatagttgtt tataagtatt gggatagata ccagttaatt tttactttta
     1261 gcttgataag caagtacatt cgtaataatc gggtttcatt gccttgttca atatggtaga
     1321 gaggtacacg gccagttcta agggaacgta atttcggtgg gtatttgatt taatttgtat
     1381 agtatgtatt acaacaaacg gacgtatttt cttaccgaat cgttgtaaat gtgttatatt
     1441 gcatggaaga tgtagtggca atatagtttt agtttaacta accatttttc caatgttacg
     1501 atacctcttt ctgctctgtt gttttttttc gtatgatgcc tcagcccgta tcttttatat
     1561 tgatccggta aatggcagta tggataatga tggttcgtat gattacccct ggcgcaccct
     1621 ggaggaagtg gtggcggccg accttattca atcctattct tatacacccc tgccttatga
     1681 cccgaatagc agtaccttaa tcatcaagaa tgagaatggc cccgttgagc cgggggacac
     1741 gctgatgctt agaagcggca tgcatggaga gttcttgctt cggggctaca ttaatccaaa
     1801 aatgatcacc atcatggctg acgaaggtca taagccaatc cttaagcaga tccgacttca
     1861 ggcctgtaag aactggcgct ttgaaaacct aattattagt accgaaccat atggatatta
     1921 tctcaatgac aggctagttt ttctggagac gcacggatgg cacggaccga catccggaat
     1981 cagcatcctg aactgtgaga tatatagcgg ggaacagccc tgggagactg cagaagaatg
     2041 gcttgccaat gccagtagcg ggatcatcat cgaaggggat tccatgatta ttgaaaataa
     2101 ttatttgaaa aatgttgtca tgggcataac agcctccggc gatcatatcc atgctatcaa
     2161 caatgtcatt gaaaattttt cagccgacgg gatgaggatg caaggcaatg acatcctgtt
     2221 tgaaggcaac accatcatga attgctacga tgtcgatgaa aatcatgacg acgggattca
     2281 atcctttatt attggagaag gtgtattccg aaacaacatc atccgctcca acatcatcct
     2341 caactatgtc gatcaggatc agaaactcct tggcgcactt cagggaatcg gctgctttga
     2401 cggattttat gaaaactggt tgatagaaaa caacgttgtc attgtcaatc actggcatgg
     2461 aatcacttta ctgggagcac gaaactgcac catccgcaac aatactgtgt tggatccgac
     2521 tcccgatata cgcccggggg gctcctggat tatgatagac agccacaaag atggcaggcc
     2581 gagtgaaggt tgtatcgtcg ccaataatgt cgccaattcc atcaatgcaa atgcgcaggt
     2641 aaataataat gtggtgatgg acacctatga ggcgtatggt tcaaatttta tggattacaa
     2701 aagttttgat tttcatctga aggaaggcag cattttaatt gattcagggt cgttgcaaaa
     2761 tgctccggaa acggacatcg atggggttgt ccggccacaa ggaggtggcg tggacatcgg
     2821 cgcttatgaa tggattgtta tgacttcaga aagacataca atacagaatg atatcagaat
     2881 atttcccaac ccctcaaccg gtcatatcca tgtagagcat cttgaaggat caggggtagt
     2941 tgaagtttat gatatgcggg gacataggtt gcatcaggaa aaatatacct cacaatcact
     3001 ccggttgaat ctgcaagacc tgaagccggg agtctactgg atcaagatca ggagtgagag
     3061 gattggaacc tttcatgcct gtcgttttat aaagctttag aaataagcga aggaggatta
     3121 gatatattat gatttaggcc ttgaatttac ctcaaaagtt gattgataat ggcttttaat
     3181 caaagtgtgt aaacaggcat aacatatcgt tgatatgggt cgttgctgta aaatttcctt
     3241 ttaaatggat aaatccttat ccccctattt aaagaatatt cattccattc attgtaccta
     3301 cagcggcaaa acacagccat ttcaaagccg tcccggaaat attcgctccg ggcgtatgat
     3361 ttagaagtga tggtgatatt gcagctatta ttgtttaaaa aatggattgg tgattgaaat
     3421 attttgactt ctttcctcca agaagcatag gcactctaca tgtccacctc agacggaaga
     3481 aagtgaaaac ctatttattt gtatcgtttt tggcaaaaaa atattttggc agactccata
     3541 ccggagagga gctatcaggc tgaaacaaag aagtatccat gcggttagaa aatgcagttt
     3601 tagttccgga aattttaatc agacagttag aaaaacacat aattttgacc gctatgaaag
     3661 taaaacagat tgacagagta gaattaaaaa gaagaggctt catttcttcc ttcagtggaa
     3721 gttcagggat tatttattcc gacgggtttg tctatattat tgctgacaaa tccaatgtac
     3781 tatcagtctt tgacgtcaat gatcaaacgc tcaaaaagga aatcagcctt aaagcggatg
     3841 gatcgctgga agctaatgtg gagaaaaagt acaagccgga ttttgaggct ttctctcgtc
     3901 atggcaatcg gtattttatc tttggttcgg gatcggcaaa aaacaggttt gatctggtca
     3961 tacttgatga aaggtttgat ttcgtcgaac ggctatccat caagaatctt tatctggcaa
     4021 tgatggaatc gaccggaatt gaccgggaca actttaatat tgagggtgta atattagagg
     4081 aagattttgc ccttttcttt aatcggggta acgggcctga tcggaaaaac ggaataatca
     4141 aagtcagaaa ctggctgacc gatgagccgg aagtggctgg ttattcaagt atagtgttgc
     4201 cggatatcaa tggccggcat gcagcttttt ctgacgccat cctccatgat ggctgtatct
     4261 gtttcctggc aaacggggaa gacacgcaat cggtgtatga tgacggggcg gtcgcgggaa
     4321 gcgcattcgg aatgatggat cttagtacat ttgaacttct ggaatttcac atcattgatc
     4381 atagtatgaa gctggagggg cttgcagtat ataaggatct gaaggaagag cacaaaatgg
     4441 ttttcattgt atgtgacgat aacgattttg aacatcaaac catcatctcc caacttactg
     4501 tgttttggcg ggattgattg gtcagaatta agccggcaat tacggactga tcacattttc
     4561 gatgaaacac taaaaatccg gcaataaggt aatcataaaa ttacctgtta tataactcat
     4621 acatatcatt tctttaaatg ttccgatcag gtagatcgaa aatgtcagat cgggtcatca
     4681 caatcccgat tgctttttcg gagctttcgt tcataacaaa atcggcttcc ttgtttcccc
     4741 tcaccggtta ttttttttaa gctcctgaat tcgattgtct gaaaatactt taattttatc
     4801 gaataaaaag cgatatttgg aatattctat tgtcctttat tgttgaaaaa agaaatagcc
     4861 aggtaatttt acaacaagct gtatttattg aaaaagatga atgtaccaaa taatacatat
     4921 ccaatgagaa acaggagtat cgcgtgcata tttgccctgt ttttaggagg gataggtgtc
     4981 cataagtttt atcttggcaa ggtaggacag ggcgtttttt atgcaatttt ttccatgact
     5041 tttatcccgg tcttcctggg ttttatcgat tttatacgcc tggcgacaat gagcgaggat
     5101 gaattcaaca ggttgtacaa tgattacatt ccgtttacta cgccaaagga atggcaaaca
     5161 aagcatcgtt acagaaatgc gaagcaggat acggtcgtct ttgagaatcg ccggaacgaa
     5221 ccgggcaggg acaaagacag tttcagtcaa aaagaacgaa gaaaaaagcg accgatgccg
     5281 ttgaccgcca accgggctta taaagatggt gtgaagttgc tggaagacta taggtatcgg
     5341 gaggctctgg agcagtttga acgtgcacga aaggcaagcg gggatcatcc ggagatactt
     5401 tttaggatgg catgttgtaa tgcagttctg gagcag
//
LOCUS       DAOQQN010000220         5406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6771_length_5406_cov_5.091385, whole genome shotgun sequence.
ACCESSION   DAOQQN010000220 DAOQQN010000000
VERSION     DAOQQN010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6771_length_5406_cov_5.091385"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLG24_08765"
     CDS             complement(<1..278)
                     /locus_tag="PLG24_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25024.1"
                     /translation="MSIGSRINYFLKRLRKEYKIIVLDEASMVQKKAILTTGMKTLGV
                     VVLLLIIFGVLTTLLTTQTGLLRYLGPRPRAILGNDYLSLKSQIDSLQ"
     gene            complement(314..970)
                     /locus_tag="PLG24_08770"
     CDS             complement(314..970)
                     /locus_tag="PLG24_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25025.1"
                     /translation="MLPLKIIGTQFITHNVKQITLEKPPGFIYRPGQSGHFSVNEEGW
                     VDKIRPFTFTSLNDWPYLELIIKIYKEREGVTNRIGKLHIGEELLLHDVFGSIEYKGP
                     GIFIAGGTGITPFIAIFRALYYSQNLRSIGLIYSNRTIDDIILGQELFKMLGPAYVNV
                     FTRQGVIGFKERKIDRNLLIELIVNFETRFYVCGPKNFTEEISSILIDLGVNPQALII
                     "
     gene            complement(1177..1686)
                     /locus_tag="PLG24_08775"
     CDS             complement(1177..1686)
                     /locus_tag="PLG24_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HMZ25026.1"
                     /translation="MNKPLYEFFTSDHRRIEDMLDKATENPDDIRMDYYHEFRTGLLK
                     HIKMEEKILFPAAQKANNGKPIPLAARLRLDHGALTSLMVVPPNAGVIRVIRHILERH
                     DVLEEEPGGMYDICEGLTEKETEYILIQLEAVTEVPVHPHNLAGYALEATKRAVERAG
                     YDYDTIAND"
     gene            complement(1853..2209)
                     /locus_tag="PLG24_08780"
     CDS             complement(1853..2209)
                     /locus_tag="PLG24_08780"
                     /inference="COORDINATES: protein motif:HMM:NF039419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6370 family protein"
                     /protein_id="HMZ25027.1"
                     /translation="MRLVVTFLAFFIFSAVGYGQIATTVNAKPEPGKKLMTVEASCGE
                     CNFGMDGNNCDLAVRIDGKPYFVDGVAITEFGHPHDKNGFCVAIRKAEVQGEVVNGRF
                     KATYFKLLGIDKKDSK"
     gene            complement(3006..4358)
                     /locus_tag="PLG24_08785"
     CDS             complement(3006..4358)
                     /locus_tag="PLG24_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ25028.1"
                     /translation="MKYINSNSINTNPTGDNHSLYFNQKAIKKTFFAFIALLLLLLHA
                     ACQSDTGHISDKAAGDKVEAAAHNHREMQPMVTSLSLEQMKTIGLEFGTLQYKELAAT
                     FRANGMLTVPNNHKGHATSLYSGVIKSIPVQIGDYIRKGQVIATITNPQFIQLQEEYL
                     TLNSKIILAEQELQRQNELNQENAGTLRNLQSATAELNTLKTRKASLTEQIKMMGINP
                     ATITNQSLRSVLSVTSPISGTVSKLYGKIGSYVDVSSPVAEIVDNSSLHLDLHVFERD
                     LGLLKVGQIIHFTITNNPVNEYDAVIYSIGTAFENDSKTIPVHAAVKGNKAGLIDGMN
                     ITGVVSLDKATTQAVPDGAIVESEGKFFIFIQTDKEPEDHQHEGEDDGHGHGIATVEK
                     KHLPTDMNFEKIEVAKGVSNMGYTALTLVKDIPADAKIVTKGAFFINARLTNAGEDGH
                     "
     gene            complement(4369..>5406)
                     /locus_tag="PLG24_08790"
     CDS             complement(4369..>5406)
                     /locus_tag="PLG24_08790"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ25029.1"
                     /translation="IQLGQYSSIRFDNAFSVSQNIPFPTVFKAKREFAESVEAGKDIQ
                     AKVSINELRHSVRKMYHSIQCFINIERQLMYLDSLYLEFTEVAKLRHKTGDTKMVDVS
                     TAETKRGEIDLKIKQNTVNMSNAYHNLRVLLNVRDSIRILPEEDYSPLTLSLYTDTLA
                     LAHHPTIMAYYLESLILEKSKAVEKAQGLPEFTVGYTNQSLIGNQIIDGQEHFFGAGK
                     RYGYYTAGIAIPLTFGATKARIQSIEYSRAASEANAIQQQNDLQAQLQSLIRQYEHCL
                     LQYKHYNEKALPNAGEIVRAARLGYRTGDISYMEYLYALQTAADVRFNYIESICQLNQ
                     IIIEIYRLLNQ"
BASE COUNT         1532 a         1189 c          992 g         1693 t
ORIGIN      
        1 ttctgcaggc tatcgatttg gctctttagt gaaaggtagt cattgcccaa aatagcccgg
       61 ggccttggtc caaggtacct aagcagaccg gtttgagttg taaggagggt cgtcagaaca
      121 ccaaatatta ttaataaaag aacaaccact ccgagtgtct tcatgccggt cgtgagtatc
      181 gctttctttt gtaccatgga agcttcatca agaactatta ttttatattc cttgcgaagt
      241 cgctttaaaa agtaatttat tctagatcct attgacatta taatttatta aggcctgaac
      301 actcaacagc caatcagata atcaatgcct gaggattaac ccccaggtcg atcaagatac
      361 tgctaatctc ttcagtgaaa tttttagggc cacaaacgta aaatctggtt tcaaagttaa
      421 caattaactc aatcagtaaa tttctgtcta tcttacgttc tttgaaacca attacgccct
      481 gtctggtaaa tacattgaca taggccggtc caagcatttt gaacagttcc tgccccagaa
      541 tgatatcatc aatagttcga ttggaataaa tcaaacctat acttctaaga ttctgactgt
      601 aatacagggc tcgaaaaata gcaatgaagg gcgttatgcc ggtgcccccg gcaataaaaa
      661 tgccaggacc cttatattcg atggagccaa atacgtcatg caataatagt tcttctccta
      721 tatgtagttt tccgatgcga ttggttacgc cttctctttc tttgtatatt ttaataatca
      781 gttcaagata gggccaatca ttcagtgatg taaaggtaaa agggcgtatc ttatccaccc
      841 aaccctcttc atttacagaa aaatgacctg attgaccggg acgataaatg aaccccggcg
      901 gcttttccag agttatttgc tttacattat gtgttatgaa ttgtgtaccg attattttta
      961 atggcagcat agcacctaac ttgtttaaat aaggattcaa ccggcaaatg gaagattatt
     1021 actaatactc ccaatagtca atttttatcc ggagctaata ttgaatctga aatttcccgc
     1081 tttgaagtcc taattcaaag atataaaaac atccggaaat cagccttcaa ttctatacaa
     1141 ttgcaccatg ccacttccgg tttggaaagg attttttcaa tcatttgcaa tggtatcgta
     1201 atcgtaacca gccctctcta ctgcacgttt cgtggcttct aaggcatatc ccgccaaatt
     1261 gtgaggatgt acaggcactt cggtgacggc ctccaattga atcaaaatgt actctgtttc
     1321 tttttctgta agtccttcgc aaatatcata catacctccg ggctcttcct caagtacgtc
     1381 atgcctttcc aatatatgac gaataactct gatgacgccg gcattaggcg gcacaaccat
     1441 aagggaggtt agcgccccat gatcaagacg gagcctcgct gccaaaggaa taggtttacc
     1501 attgttggct ttctgagcag caggaaaaag tatcttttcc tccatcttga tatgcttcaa
     1561 caaacccgtt ctgaactcat gatagtaatc cattctgata tcatccggat tttcagttgc
     1621 tttgtcaagc atatcttcta tcctccgatg atcggatgta aaaaattcat atagcggttt
     1681 gttcatactc ccaataattt actgaatact atgtttcaaa taaaaaacac ttcccccttc
     1741 ccgaatggaa caccactcct accaggcact ttaggttcaa cttgtaacat tgaccgatct
     1801 gcgtatttca cttcaaatga agctgaatgc tgctttaatg tataaaaaaa atttatttag
     1861 aatccttttt gtctatgccc aacagcttga aataggttgc tttgaagcgt ccgttgacca
     1921 cttcaccctg aacttcagct tttctgatag cgacacaaaa cccgttctta tcatgaggat
     1981 gaccaaactc tgtgatagca acgccatcaa cgaagtaagg cttaccatca atacgtacag
     2041 ccagatcaca gttattgcca tccattccaa aattacattc accacaagaa gcttcaacgg
     2101 tcatcaattt cttaccgggt tccggcttag catttacagt tgtagctatc tggccatatc
     2161 caactgcaga aaatataaaa aatgccaaaa aagtaactac taatctcatc tctaatcttt
     2221 tttggcaaag ataatcaatg aatcccgctt gtcgctatga ttttcaaaaa aaaagataat
     2281 gtaatcctat attaattgag cagtaactaa ttggtgacaa atattccata acattttgat
     2341 aatcttcgta ataaataata caatattttg atatttatac gggattttgt accagtctcg
     2401 tttaaatctt gcataaattc tatcattccg caaactaaaa acctgaccag tcggtatatt
     2461 atcactgcgt aatgatatgt cctgtcaaga ccatccatca taccattaag tcaatatagc
     2521 taaccggaat attttggatc atgccattcg tcattaatgc agcaaataag ttggatggcc
     2581 ggccaaaccg acttgcctga tgattggatt gttgttatcc ggatgtttca aatgcgagaa
     2641 taaatgaaca gggaagtgac aaccttttca atcaaatttt atgttcacaa tagccccagt
     2701 tatttcctac agaatgatgc acaattggga ataatctttt tgaaagtttt acaaagttca
     2761 ttgccatcca ttttcaaccc attattgatt ttgcaaacta ttctatcatc gatgtttggt
     2821 ccaggtcttt ccaaaataaa atttgaactg tatttttaat gattgaccac ctataatctc
     2881 tgtatgatta atctgtccct cctccgacac ccaacttgca aagaagcagc acccttgtca
     2941 gtccaatttt cggggaatcg acaagatatt taacttttcc ttccccggaa attttcattg
     3001 tatcatcagt gtccgtcttc accggcattc gtgagcctgg cattgataaa aaaagcgcct
     3061 ttggtgacta ttttggcatc agcaggaata tcctttacaa gtgtcaatgc tgtatagccc
     3121 atattggata cacctttggc tacctctatc ttttcaaaat tcatatcggt tggtaaatgc
     3181 ttcttctcta cagttgctat gccatgtcca tgtccatcat cttcaccttc atgctggtga
     3241 tcttcaggct ccttgtccgt ttggatgaag atgaaaaatt tcccttcgga ttcaacgata
     3301 gcaccatcag ggactgcctg ggttgtggct ttatcaagac ttaccacccc ggtaatattc
     3361 atcccatcaa taagtccggc cttattccct ttgacggctg catgtaccgg aatggttttg
     3421 ctgtcatttt caaacgctgt cccgatactg tatatcacgg catcatattc gtttaccgga
     3481 ttgttcgtaa ttgtaaaatg gatgatctgc ccaactttca acaatcccag gtctctttca
     3541 aatacatgta agtcaaggtg cagcgagcta ttatccacaa tctctgcaac gggagaagaa
     3601 acatcgacat agctgcctat cttcccatat aatttactga ccgtacccga aatagggctt
     3661 gtaacggaca ataccgatct taaggattga ttggtgatgg tagcagggtt gattcccatc
     3721 atctttattt gttctgtcaa agaagctttt cgcgtcttca aggtgttcaa ttccgccgtc
     3781 gcgctttgaa ggtttctgag agtaccggca ttttcctgat tcaattcatt ctgacgttgt
     3841 aattcctgtt cagccagaat aattttactg ttcaacgtca gatattcttc ctgcaactgg
     3901 ataaactgag gattggtgat ggtagcgatt acctgacctt tcctgatata gtcccctatc
     3961 tgaaccggaa tagactttat cacaccactg tataaggacg ttgcatggcc cttatgattg
     4021 tttggaacag tgagcatgcc atttgcccgg aaggttgccg ccaattcctt atattgtaag
     4081 gttccgaatt caagtccgat tgttttcatt tgttccaaag acaatgaagt gaccataggc
     4141 tgcatttccc ggtggttatg ggcagccgcc tcgaccttat caccggcagc tttgtcggaa
     4201 atatgtccgg tatctgattg gcacgcagca tgcaacagaa gtaataacaa tgcgataaat
     4261 gcaaagaatg tcttcttaat ggccttttga ttaaaatata aggagtgatt atcgcctgtt
     4321 ggatttgtat tgatactatt tgaatttata tatttcattt caggaaaatt attgattgag
     4381 gagtctatag atttcgatga tgatctgatt gagttggcag atgctttcaa tataattgaa
     4441 tcgtacatca gccgcagtct gcaaagcata caaatattcc atataactta tatcaccggt
     4501 gcgatagccc agtcgtgcag cccttacgat ctcgccggca tttggcaatg ctttctcatt
     4561 ataatgtttg tattgtaata ggcaatgctc gtattgccgg atcaaacttt gcaactgcgc
     4621 ctgcagatca ttttgctgtt gaatggcatt tgcttccgaa gcggctctgg aatattcaat
     4681 ggactgaatc cgggctttag tggcaccgaa agtcagcggt attgcaattc ctgcagtgta
     4741 atagccatac ctcttaccag ctccaaaaaa atgttcctgt ccatcgataa tctggttgcc
     4801 aatcagagat tggttagtat agcctaccgt aaattccgga agtccctgag ccttttccac
     4861 cgctttgctc ttctccaaaa ttaaggattc caaatagtat gccattatgg tcggatggtg
     4921 agccaatgcc agggtgtcgg tatagagaga aagtgtcagt gggctatagt cttcttccgg
     4981 aagaatccgg attgagtctc tgacattcag caacacacga agattgtgat aggcattgct
     5041 catattaact gtgttctgct ttattttaag atcaatctca ccccgcttgg tctcagccgt
     5101 actgacgtca accatttttg tatcacctgt cttgtgacga agcttagcca cttcagtaaa
     5161 ttccagataa aggctatcca gatacatcaa ttgcctctcg atgtttataa aacactggat
     5221 ggaatgatac atctttcgca cactatgtcg gagttcattg atggaaacct ttgcctgaat
     5281 atccttacct gcttcgacag attctgcaaa ttccctcttc gccttaaata ctgtcggaaa
     5341 tggtatattt tgagaaacgc tgaaagcatt gtcaaacctg atgctactat actgccccaa
     5401 ttgtat
//
LOCUS       DAOQQN010000221         5402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6782_length_5402_cov_4.899196, whole genome shotgun sequence.
ACCESSION   DAOQQN010000221 DAOQQN010000000
VERSION     DAOQQN010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6782_length_5402_cov_4.899196"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PLG24_08795"
     CDS             <1..1137
                     /locus_tag="PLG24_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HMZ25030.1"
                     /translation="PNTVIVKNFSFSPSELTIKEGETVTWNFEEGHHNVNGKQSAFPD
                     NPDSFFSGPAADAPFTYSFTFTKVGDYSYQCDPHATSMKGKIHVLPKTPSVSYPVRTI
                     GEISQVDGQGVADSLGRLCEVKGIVYGVNMRATGISTTIIDSKRDGIGLFSNSKTFGY
                     TVNEGDEVTVRGKVDQFNGLTQIQIDTMWVNSSGNTIFTPRTTTVLGEEEESDLVMLT
                     GMSLKDPGQWKKGSTFNATITNGTNEFTLRVVNTTTLSQIDAPSGSFTVVGIGGQFDS
                     SNPYTDGYQLLPRKSEDLVPTVSTRNLIGDQVSVFPNPSQDQLFVSSSLNILKLEVLS
                     ETGKWISTESNNSRFVDIHNLLPGNYLLRITTAEGIGVKKFIKL"
     gene            1205..2023
                     /locus_tag="PLG24_08800"
     CDS             1205..2023
                     /locus_tag="PLG24_08800"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMZ25031.1"
                     /translation="MKVFLWIGVLIASVPFSMKGEYNVRIYSEKENGEVRYYVDNNEW
                     CPVSVVFDFELKNMKSSKGPEFTVVIPAQSSKFPVTTLKPYKENELYGFKYKTTIILG
                     NVHAVHDQGYVYELPFPPGKTYKIFQGYNGRLSHVGQNALDFSMAVGDSVLAARSGVV
                     VDVVQHNHQSCMDPGCEKFNNYILVYHEDGTFGEYAHIRKDGSLVMPGDSISAGCAIA
                     LSGDVGFTSGPHLHFIVYLPGKKGKKSVKTLFRTQNNTSEYLVEGASYYRTKNN"
     gene            2028..2744
                     /locus_tag="PLG24_08805"
     CDS             2028..2744
                     /locus_tag="PLG24_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMZ25032.1"
                     /translation="MKKSALLFLYVFIGIVCLNAQDAKVKYLLSAEQFSDKLKENPQA
                     TLLDVRTPDEFAKSHLKDAINIDWRSKGFLNDASALPKDRQVYIYCLSGGRSHSAADK
                     LRTQGYTVYEMEGGILQWQKAGLPEVKGDGKKGKDGMSLNEFDKMVSSHPYVLIDFSA
                     PWCGPCKKMKLYIDAIEKEMNGALKVVVINTDENMTLVKKMKIYSIPYLQLYRDGKLK
                     WENMGFTDEKYLREVVNDFK"
     gene            complement(2806..3471)
                     /locus_tag="PLG24_08810"
     CDS             complement(2806..3471)
                     /locus_tag="PLG24_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ25033.1"
                     /translation="MGDQRLYALRDISFKVNRNEYVALMGPSGSGKSTLMNILGCLDT
                     PTSGKYLLNDHDVSHMEDKELAHVRNKEIGFVFQTFNLLPRVSALENVAVPLIYAGVS
                     RKERLERAKVAMDMVGLSDRMDHKPNELSGGQRQRVAIARAIVNDPAIILADEPTGNL
                     DSKTSLEIMAIFEKIHSSGNTIILVTHEPDIAEYAHRIIRLRDGLVENDEQNAHPKRQ
                     LSR"
     gene            3664..4305
                     /locus_tag="PLG24_08815"
     CDS             3664..4305
                     /locus_tag="PLG24_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HMZ25034.1"
                     /translation="MENEKPIPKHIAIAGNIGAGKTSLSALLGKHFDWEVHYEDTEDN
                     PYLSDFYDDMTRWSFNLQIYFLNNRYRQILDIQNGDHTVIQDRTIFEDAHIFAPNLHE
                     MGLMSKRDFDNYFDLFSTMSSQVKAPDLLIYLRASVPTLVDHIQTRGRDYEGNMSLDY
                     LKKLNQRYEEWIETYNHGPLLIVNTDRLNFIQNKEDLGTIIEMIDGELNGLFS"
     gene            4631..>5402
                     /locus_tag="PLG24_08820"
     CDS             4631..>5402
                     /locus_tag="PLG24_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HMZ25035.1"
                     /translation="MSSKYNLRGVSADKEDVHAAIRSLDKGLYPSAFCKILPDLVGND
                     PDFCNIMHADTAGTKTSLAYMYWKETGDISVWKGIVQDAIVMNTDDMACVGCVDDIVL
                     SSTIGRNKALIGKEVLSALIDATSEFIDNMKNLDVNIYLSGGETADVGDIVRTADVGF
                     TAFARMKRSDLIVNRIRPGQVIVGLASYGKASYETEYNSGIGSNGLTMARHELFGGDY
                     VDRYPETFAPQTNREYVYSGKGRLTDEINIAGQSYPLGK"
BASE COUNT         1607 a         1006 c         1194 g         1595 t
ORIGIN      
        1 cccaatactg ttatagtaaa gaatttttca ttttcacctt ctgaactgac tattaaagag
       61 ggagaaacgg ttacatggaa ctttgaggaa ggacaccata atgtcaatgg taagcaatct
      121 gcctttccgg ataatcccga ttcctttttc agcggtcctg ctgctgacgc gccatttact
      181 tatagcttta cctttaccaa ggtaggtgat tatagttatc aatgcgatcc gcacgcgact
      241 tcgatgaagg gtaaaatcca cgttttgccg aagacacctt ctgtatccta tccggttagg
      301 acaattgggg agattagtca ggttgacgga cagggtgtcg ctgattccct tggaagactc
      361 tgtgaagtaa agggaatcgt ctatggagta aacatgcggg caaccggtat tagcacaact
      421 attattgact caaaaaggga cggaatcgga ttattcagca acagcaaaac atttggatat
      481 actgtgaatg aaggtgatga agttacagtc agagggaagg tcgaccagtt caacggcctg
      541 actcaaattc agattgatac aatgtgggtc aattcatccg ggaatacaat cttcacgccc
      601 aggacgacaa cagttctggg agaggaagaa gaatctgatc tcgtcatgtt gacaggtatg
      661 tcgctcaagg atcccggtca atggaagaaa ggctctacat tcaatgctac gataaccaac
      721 ggaacaaatg aatttaccct gagggtagtg aatacgacaa ccctttctca aatcgacgct
      781 ccatccggat cctttactgt agtgggtatt ggcggacagt ttgattcatc caacccttat
      841 actgatggat atcagttact cccacgaaag agtgaggatc ttgtacctac cgtaagtact
      901 agaaatttaa ttggcgatca agtgagtgtt tttcccaatc cttctcaaga tcagctgttt
      961 gtgagcagta gtctgaatat tttaaaatta gaggtactca gcgaaaccgg aaaatggata
     1021 agtacagaaa gtaacaattc ccgttttgtt gacattcata atttgttgcc cggaaactac
     1081 ttgctgagaa ttaccactgc ggagggtatt ggcgttaaga aattcataaa actatagaat
     1141 tatttttaga ataataatat tttaagctgc attcgggtta tcattcgaat gtagcttatg
     1201 ttttatgaag gtatttcttt ggattggtgt tttgattgct tctgtgccat tttcaatgaa
     1261 aggcgaatat aatgtcagaa tatattctga aaaagaaaat ggggaagtca gatactacgt
     1321 tgataataac gagtggtgtc cggtgtcggt ggttttcgat ttcgaactta agaatatgaa
     1381 atcgtctaaa ggacctgaat ttactgttgt catcccggca caatccagta agtttcctgt
     1441 aactactttg aaaccttaca aagaaaatga attatatggc tttaagtaca aaactacaat
     1501 tatcttaggc aatgttcatg cagtgcatga tcagggatat gtatatgaat taccctttcc
     1561 acccggtaaa acctacaaaa tatttcaggg atacaatggc agattatcac atgttggcca
     1621 gaatgcgttg gatttttcta tggctgtagg cgattctgtt ctggcggcca ggtcaggcgt
     1681 tgttgttgat gttgtccaac acaaccatca aagttgcatg gatcccggtt gcgagaagtt
     1741 caataattat atccttgtct atcatgagga tggtactttt ggtgaatatg cccatatcag
     1801 gaaggatggg agccttgtga tgcccggaga ctcaatcagc gccggatgtg ccattgcctt
     1861 gagtggagat gttggattta cgtcaggccc acatcttcat tttattgtat atttgcccgg
     1921 caaaaaaggt aagaaaagtg tcaagaccct ttttcgcacc cagaataata catcggaata
     1981 tctggtggag ggagcatcat attataggac aaaaaacaat tgataaaatg aaaaaatccg
     2041 cacttctgtt cctttatgta tttataggaa ttgtctgcct gaatgcccag gatgctaaag
     2101 taaaatatct tctgagtgct gaacaatttt ccgacaagct taaggagaac ccacaggcta
     2161 ctcttttgga tgtccgtact cccgacgaat ttgccaagag tcatttaaag gatgcgatca
     2221 acattgattg gcggagtaag ggatttttaa atgatgcgtc tgcacttccg aaggatcgtc
     2281 aggtatatat atactgcctt agtggcggtc gtagtcattc tgcagccgat aaattgcgga
     2341 cacaaggtta tacggtttat gaaatggaag gcggtatcct tcaatggcag aaagccggac
     2401 ttccggaagt aaagggagat ggtaagaaag gaaaggatgg gatgtcactc aatgaatttg
     2461 ataaaatggt ttcttcacac ccttatgtcc tgattgattt cagcgctccc tggtgcggac
     2521 cttgcaaaaa aatgaagctg tatattgatg caatagaaaa ggaaatgaat ggagctctta
     2581 aagtcgttgt gattaatacc gatgaaaata tgactttggt taagaaaatg aaaatatact
     2641 ccatccctta cctgcaactg tatcgtgatg gaaaattgaa atgggagaat atgggcttta
     2701 cagatgagaa atacctaagg gaggttgtca atgactttaa gtgattatca gtccaaatga
     2761 ttgttaagtc acagattacc ttttaaattt tattagtaaa tttacttatc gggataactg
     2821 tctttttgga tgagcattct gttcgtcatt ttcgactaat ccatctcgca aacgaatgat
     2881 tctatgagca tattcggcga tatccggttc gtgagtcact agaatgatgg tgttgccgga
     2941 tgaatggatc ttttcaaaga tagccatgat ttcaagagaa gtctttgagt caagattacc
     3001 ggtaggctca tctgcaagta taattgccgg atcattgact atcgcacggg caatggcaac
     3061 ccgttgtctt tgtcctcctg aaagctcatt cggcttgtgg tccatcctgt cggaaagccc
     3121 taccatatcc attgctacct tagccctttc aagtctttcc ttcctggata cacctgcata
     3181 tatcaatggt acggcaacat tctccagtgc cgaaaccctg ggcagcaaat tgaaagtctg
     3241 aaagacaaag cctatttcct tgttccgcac atgagcgagt tccttatctt ccatatggct
     3301 tacatcgtga tcattcagca gatacttgcc tgatgtgggt gtatcgagac agccaagaat
     3361 attcatcaaa gtggatttac ctgaccctga tgggcccatg agtgccacat attcatttcg
     3421 gttgactttg aaactaatgt cccgcaaagc gtaaagtcgt tggtctccca tgatatagac
     3481 cctggaaaga tgttctaatt ctatgacatt ttgcgtattc aaatcgttca agttgattgc
     3541 ttttatctgc aatattagtt tgaaatctca ataaaaccag gattattcga tgtgaatcaa
     3601 aaaattatcg tcaaaaatca ccttttgctt tgcaaaccca ttttctggaa gtaaatttga
     3661 cagatggaaa atgagaaacc aattccaaaa catattgcaa ttgccgggaa tatcggtgcc
     3721 ggtaaaacgt ccctttccgc tctgttgggt aagcatttcg actgggaagt ccactatgag
     3781 gatacagaag acaatcctta tttgtctgat ttttatgatg atatgactcg atggtccttc
     3841 aaccttcaga tatattttct aaataatcga taccgacaga ttctggatat tcaaaatgga
     3901 gatcatacag tcattcaaga tcggacaata ttcgaggatg cccacatttt tgccccaaac
     3961 cttcatgaga tgggcttgat gtcaaaaaga gactttgata actattttga tttgttttct
     4021 actatgtcat ctcaggtgaa ggcacctgat cttctgatct atctgagagc gtccgtgccg
     4081 acactcgtcg atcatatcca gacacgaggg agggattacg aaggaaatat gagtcttgac
     4141 tatctgaaaa aactcaatca acgatatgaa gaatggatcg agacctacaa ccacggacca
     4201 ctattgatcg tcaataccga caggttgaac tttattcaaa ataaagaaga cctgggtaca
     4261 atcattgaaa tgattgatgg tgagttgaac ggtctgtttt cataattaag gtgttgcgtt
     4321 gatagcagtg gccggaccgg caatattatt cctgctcggt atctgtttcg tcgagagaag
     4381 caagaaagat aatattacta aaaatagaat aaacgcaaga attgcatttt ttttcatcac
     4441 ctttgcctgt gataattgat acattgacct atacatgata aataggtttt aggataataa
     4501 ttgttgtatt ttcaacggaa aaatgacgcg aagtagttca taatcgggca attcaatatt
     4561 gtaatttaag atttctttga cgcaaagata acatattatt tttatctata atatataatt
     4621 tgtatttttt atgtcatcaa agtataattt gaggggagta tccgctgata aagaagatgt
     4681 tcatgccgca attcgcagct tggacaaagg tttgtacccc agtgcttttt gcaagattct
     4741 tcctgatttg gtaggcaatg atccggattt ttgcaatatc atgcatgctg atacagccgg
     4801 caccaaaaca tctctggcat acatgtactg gaaagagacc ggtgacatct cagtctggaa
     4861 gggcattgtt caggacgcta tagtgatgaa tacagacgat atggcttgtg ttgggtgtgt
     4921 ggatgacatt gtcttgtcca gcacaattgg gcggaataag gccttaattg gtaaggaagt
     4981 tttatcagct ctgattgatg ccacctctga attcattgat aatatgaaaa atctggacgt
     5041 caatatatac ttgtccgggg gcgaaacggc agatgtagga gacattgtta ggacagccga
     5101 tgttggattt actgcattcg ccagaatgaa aaggtcggat ttgatcgtca atagaatccg
     5161 tcccggtcaa gtaatcgtcg gattggcctc ctatggcaag gcttcttacg agaccgaata
     5221 caatagtgga attggctcta acggcctgac catggcaaga catgaactct tcggtgggga
     5281 ctatgtagat aggtatccgg aaacctttgc ccctcagact aatagagagt atgtgtatag
     5341 cggtaaaggc aggttgacag atgaaataaa tattgccgga cagtcgtatc ctttgggtaa
     5401 gc
//
LOCUS       DAOQQN010000222         5393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6805_length_5393_cov_5.391532, whole genome shotgun sequence.
ACCESSION   DAOQQN010000222 DAOQQN010000000
VERSION     DAOQQN010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6805_length_5393_cov_5.391532"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="PLG24_08825"
     CDS             complement(<1..852)
                     /locus_tag="PLG24_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25036.1"
                     /translation="MKRLIFGILMYGFVLANSVSAQSSLDVLRLSGQNLLGTARAAGT
                     NTTMSGVGADFTTLSSNPAGIAQYRLSEIMVTPSVVDNRTKSRLADGSERPETSDSKT
                     KFRLPNLGLVIAKQNSDRMSYAFGFGINGYVDNSQNSLFRGRSKGSIVERFAALANGL
                     SLEQLDNYEAGLAWDTEALVQQRPDGSYSFDYEDYKDQELNKYQSISASNRYNDIAIS
                     GGINLNNKILVGASIGIPTFSYNERKIYQEQDDIGAVPYFDQLQFREYKSASGVGVNG
                     KLGIIFKP"
     gene            complement(1155..2204)
                     /locus_tag="PLG24_08830"
     CDS             complement(1155..2204)
                     /locus_tag="PLG24_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25037.1"
                     /translation="MFGTIAFAQFDDVYFNPKKDVLPGSYTSSSNSKATDSDYGYSYN
                     SGNTGAINGGYEGNNTINSDEYDDQSYEYYEDYTSRINRFHRYNVSGFGYYDPCYTGG
                     FGYNSWSFRPSVTFMYDPFFNYNRYYNNSFWGSPYYGWYNDPFFDYGYPYYGYGYGYG
                     YPYYGYGYPYYGYSPYYGGHNGWYGNGWYGDSDKNKVSRVYGARYGGAVVGGSDGYRD
                     RSKMTNGTINPNDSRSRLNNGSKPELEVRNTNTNRGRVDGSSSRPDRSNKTNNPDRGT
                     RINTERPRSTPQRETIPTRESMPSRTYEAPTRSRSTETYSPSRSGNSSGSYNSNSNSS
                     GTERGTSGSSSRSRG"
     gene            2884..4350
                     /gene="proS"
                     /locus_tag="PLG24_08835"
     CDS             2884..4350
                     /gene="proS"
                     /locus_tag="PLG24_08835"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HMZ25038.1"
                     /translation="MSKEITPRDENYSAWYNDIVIKAGLADHSAVKGCMVIKPYGYAI
                     WEKMKDQLDAMFKDTGHVNAYFPLLIPKSFLSKEAEHVEGFAKECAVVTHHRLMNDPN
                     GKGVVVDPSAKLEEELIIRPTSETIIWNTYRDWIKSYRDLPLLINQWANVMRWEMRTR
                     LFLRTAEFLWQEGHTAHATADEAIEEARTMLNVYADFAENYMAMPIIKGVKSANERFA
                     GALDTYTIEALMQDGKALQAGTSHFLGQNFARAFDVKFLTDKNTEELVWATSWGVSTR
                     LIGGLIMTHSDDLGLVLPPRLAPIHVVIVPIPKANDDIDKIALDILAQLKLKGITAKY
                     DNDEKRRPGFKFAEYEMKGVPIRIGIGARDLANGEVEMARRDTLEKSQVKLEGLVDTI
                     VNTLDLIQNNLLERSRQYRDQHITDVNSFDEFKDVLTNKGGFVRAFWDGTTETEEKIK
                     ELTKATIRCIPLDSKEEEGICVYSGKKATQKALFAIAY"
     gene            4363..4713
                     /locus_tag="PLG24_08840"
     CDS             4363..4713
                     /locus_tag="PLG24_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HMZ25039.1"
                     /translation="MSFEISGKLVKKYDVESKTATFQTREFVIVTEETYPQFIKFQLS
                     QERVNLIDAYNENDVIKVHFDLRGREWQGKYFTNLNAWKIEGVTASNSASGHPGNDVP
                     FEINDEPFENDLPF"
     gene            4773..5291
                     /locus_tag="PLG24_08845"
     CDS             4773..5291
                     /locus_tag="PLG24_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25040.1"
                     /translation="MALQAQTDSTELNISGLSIAREDDFTDWDFYGNTDDPIGYLRLR
                     WIFQKDWSEWDYRIGEETGSIKQRTKSDPNIWELRSGNVILTLKTIYTGDFNQWRITD
                     DSQTLTFSTLYENVYDGWKTSSKAGQFEIYTQWEMDPRDWIVYDKLKPEISNPFKIAM
                     IFLASYYSSPKY"
BASE COUNT         1641 a         1133 c         1057 g         1562 t
ORIGIN      
        1 aggcttgaaa attataccca atttgccatt taccccaaca ccagatgctg atttgtactc
       61 cctgaattgt aactgatcaa aatatggtac agcccctata tcatcttgct cctgatatat
      121 cttcctctca ttgtaactaa aagtcggtat tcctatcgat gctcccacca gaattttatt
      181 attcagattg ataccgccgg atattgctat atcattatac cggttgcttg cactaatgga
      241 ttggtacttg ttcaactcct ggtctttata gtcttcatag tcaaacgaat acgagccgtc
      301 aggcctttgt tgtacaagag cttctgtatc ccaggccagc cctgcctcat aattatccaa
      361 ctgttcaaga ctcagaccat ttgccaatgc ggcaaaccgc tccactatcg accccttgct
      421 cctccctcta aatagcgaat tttggctatt gtcaacgtat ccattgatgc cgaatccaaa
      481 agcatagctc atcctgtcgc tattttgctt ggctataacc aatcccaaat tgggcaaacg
      541 aaattttgtt ttactgtcac ttgtttccgg acgttcgctt ccatcggcca atctcgactt
      601 cgtacggtta tccactacag aaggagttac catgatttca gacaatctgt actgagcaat
      661 tccggccgga ttggacgata acgtggtaaa gtccgctccc acaccgctca tggttgtatt
      721 agtcccggct gcacgggcgg ttccaagaag attttgtccg gacaagcgaa ggacatcaag
      781 cgatgactgt gctgatacgg aatttgctaa aacgaaacca tacatcaata ttccaaaaat
      841 taatcttttc atggctttac taaataatat tgttaaacta tttgttgagg caatagttta
      901 ttggatcaaa aaaataccag gttgcggttt gtcattgcgc ctcattcaat agacacgcct
      961 ccctgattca tcatcttcat ttattgcctg atggatgatt attcgctttc catcaaatgt
     1021 ttaaacacca tttatcatac ccgactgtat taatgaggac agaatcagtc cggtatagaa
     1081 taaaataagg tggcaaaata tgatcgtacc accttatttg agttataaaa ataaaattga
     1141 cattatcaat actatcaacc ccttgacctg gaagacgaac cagatgtacc tctttcagtt
     1201 ccacttgaat tgctgttgga attataagaa cctgaggagt tgccggatct tgatggtgaa
     1261 taggtttcag tcgatcttga tctggtaggc gcctcatagg ttctcgaagg cattgattca
     1321 cgtgtaggta tggtctctct ctgtggtgtt gatctcggac gttctgtatt tatccttgtc
     1381 ccccgatccg gattatttgt tttgtttgac ctgtctggcc tgcttgaact tccatccaca
     1441 cggccccggt ttgtatttgt attcctcact tcaagttcag gtttggagcc attgttaagt
     1501 cggctacgac tatcatttgg gttgattgtg ccgtttgtca tttttgacct atcccgatat
     1561 ccgtctgagc cacccactac agcaccacca tatcttgctc cataaaccct gctaacttta
     1621 ttcttatcgg agtctccgta ccaaccattt ccgtaccagc cattatgccc accataatag
     1681 ggagagtaac cataataagg ataaccatac ccatagtagg gatatccgta gccataacca
     1741 taaccatagt aaggataacc ataatcgaaa aacggatcat tataccaccc atagtatggg
     1801 cttccccaaa aactattgtt ataatatctg ttatagttga agaacggatc gtacatgaag
     1861 gttacacttg gcctgaatga ccaactgtta tacccaaatc ctcccgtgta acaagggtcg
     1921 taatagccaa acccactgac attatatctg tggaatctgt tgattcttga tgtatagtct
     1981 tcataatatt cataactctg gtcatcatat tcatcggaat taatggtatt attgccttca
     2041 taaccaccat tgattgcccc cgtattgccg gaattataac tatatccata atcagaatct
     2101 gttgccttac tatttgaaga tgaggtataa gatccgggca agacatcctt tttgggattg
     2161 aaatatacgt catcaaactg ggcaaaagct atagtcccaa acattaggaa cattatcata
     2221 ggaaataaga atcgcaaagc tttcatttta atgaatttat tattaatcac aacttgttta
     2281 tcaattcaac agctaaagta ttacttttat ccccaagaat tagttaaatt attgataata
     2341 tttattttca gttagttaaa actttattta tccaaaatca ttttcacgaa aataaccact
     2401 ggtaaatgat ttgcaactat tcgccgctcc ggttctcaag ttaccgattt tattgctaat
     2461 cccccgcaat ggctctatat actattgatt cccaagtgag gcagtttgtc agattgtcat
     2521 acgccttgtc ctttgtttat gtattaattt ctttgttgca caattcagaa aacacacggg
     2581 cctcgcaatt ttgtcaaaat atcacggtta aagtgtttta tagccgaaac aaaaagatga
     2641 tattatacga taaataatta caaagatttt acacgctgtg caattcaatc aggacaaact
     2701 tattaatata tttattcgct tttatcaact aaaatttaaa gaaaatacct aatttaattt
     2761 tcttttaagg aattaattaa atttgcgcct gaatacggaa ggattattta tctgcaatac
     2821 tggaattaag acattttcag tgaagtaaag cgaagtttca atttcaacat tttaaacagg
     2881 aaaatgagta aagaaataac tccaagagat gaaaactact ccgcatggta caatgatatc
     2941 gtgatcaaag ccggcctggc cgatcattct gcggtcaagg gatgtatggt tatcaaaccc
     3001 tatgggtatg ccatctggga aaaaatgaag gatcaactgg atgccatgtt taaggatacc
     3061 ggtcacgtca atgcctactt tcctctccta atcccaaaaa gtttcttgag caaggaagca
     3121 gaacatgtgg aaggttttgc caaggaatgc gcagtggtca ctcatcacag gcttatgaat
     3181 gatccgaatg gaaaaggggt agttgtagat ccttctgcca agcttgaaga ggagcttatc
     3241 atcaggccca cttctgagac catcatttgg aatacctacc gagactggat caagtcctat
     3301 cgtgacctgc cattacttat caatcaatgg gcaaatgtca tgcgatggga aatgcggaca
     3361 agactttttc tcaggactgc ggaatttctg tggcaagaag gacatacagc acatgcaact
     3421 gcagatgaag caattgaaga agccaggacc atgctcaatg tttatgcgga ctttgctgaa
     3481 aattacatgg caatgccaat tatcaaaggt gtaaaatcag ccaatgaacg atttgccggc
     3541 gcactagaca catacaccat tgaagctttg atgcaagatg gtaaggcact gcaagcgggc
     3601 acctcacatt tcctgggaca aaattttgcc agagcttttg atgttaagtt ccttactgat
     3661 aaaaataccg aagagttggt atgggcgact tcatggggtg tatccaccag gttgataggt
     3721 gggttgatta tgacccattc ggacgacttg ggattggttc tgcctccacg tttagctccg
     3781 attcatgttg taatagttcc aatacctaag gcaaatgatg acatcgacaa aattgctctc
     3841 gatattcttg ctcaactcaa actaaaagga attactgcca aatacgacaa cgatgaaaaa
     3901 agaagacccg gattcaaatt tgccgagtat gaaatgaaag gcgtgccgat cagaatcggt
     3961 atcggtgcca gagatctggc caacggcgag gtggaaatgg ccagaaggga tactttggaa
     4021 aaatcacagg tcaaactgga aggattggtc gacacgatcg taaatacttt ggatttgatt
     4081 caaaacaatc ttttagagag gtccagacaa tatcgagatc agcacattac agatgtaaat
     4141 agttttgatg aattcaagga tgttttgacc aacaagggtg gttttgtcag ggcattctgg
     4201 gacggtacta ccgagaccga agagaaaatc aaggaactaa ccaaggctac cattcgttgt
     4261 attcctttgg actctaagga agaagaagga atttgtgtct attcaggcaa gaaagcaact
     4321 cagaaagccc tatttgcaat cgcttattaa atccaaaaaa atatgagttt tgaaatttcc
     4381 ggtaaactgg taaaaaaata tgatgtcgaa tctaaaactg ccacatttca gacacgtgag
     4441 tttgtgattg tcacagagga aacctacccc caattcatca agttccagtt gtcacaagaa
     4501 agggtcaacc tgattgatgc atacaacgaa aacgatgtaa tcaaggtaca cttcgacttg
     4561 cgaggacggg aatggcaggg taaatacttt accaatctga acgcctggaa gattgaaggt
     4621 gtgacagcaa gcaacagcgc ctccggacat ccgggaaatg atgtcccgtt tgagattaat
     4681 gatgagccgt ttgaaaacga cttgccgttt taaagataac ttccaacatc acgatgcgct
     4741 ataacctgtt gctcataatg gtttttatcg gaatggcatt acaagcacag accgattcga
     4801 cagaattgaa catttccgga ttgagtattg ccagagagga tgacttcacc gattgggatt
     4861 tctatggcaa tacagacgat ccaataggat accttcggct tcgatggata tttcagaaag
     4921 attggtccga atgggattac cgcattggtg aagaaacggg ctcaatcaag caacggacta
     4981 aatcagatcc caatatatgg gagttgcgtt caggcaatgt cattctcacc ttaaaaacca
     5041 tttataccgg agactttaat caatggcgaa tcacagatga ttcacaaacc ctaacatttt
     5101 ctaccttgta tgaaaatgta tatgatggtt ggaagacaag tagtaaagct ggccaattcg
     5161 aaatatacac ccaatgggag atggatccca gggattggat tgtttacgac aaattaaaac
     5221 ccgaaataag caaccccttt aagatcgcta tgatttttct ggcaagttac tattccagcc
     5281 ccaaatattg accgactcca cggttcaagc tgccggtaag catgattgcc agaaggtgaa
     5341 tactatgtaa ttgcccatct tgtgaatgtc gttcccttgt accaaacatt cct
//
LOCUS       DAOQQN010000223         5389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6821_length_5389_cov_4.512373, whole genome shotgun sequence.
ACCESSION   DAOQQN010000223 DAOQQN010000000
VERSION     DAOQQN010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6821_length_5389_cov_4.512373"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PLG24_08850"
     CDS             complement(<1..316)
                     /locus_tag="PLG24_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HMZ25041.1"
                     /translation="MAKVTFKFEDKQPDVTIEDVEEGVSILEVAEENDIHLNHNCGGV
                     CACSTCHVYIYEGEDDLEEISDKEEDFIDRAINPKLNSRLGCQCIILSDDAEIVVEIP
                     DQS"
     gene            658..3498
                     /gene="polA"
                     /locus_tag="PLG24_08855"
     CDS             658..3498
                     /gene="polA"
                     /locus_tag="PLG24_08855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HMZ25042.1"
                     /translation="MSSDKRIFFIDGHAMVYRAHYAFINRPLMNSKGQNVSAINGFIR
                     AIWDLIVNQKPTHLGVVFDPKGGTFRSDLYEEYKAQRDAQPEDISFAIPYIIKILEGM
                     NIPVEIIPNYEADDVIGTLAKKAEKEGFEVYMVTPDKDYAQLVSENIFQYKPARFGND
                     IEILGVEEVLKKWEIKRVDQVIDILGLMGDSVDNIPGLPGVGPKTAVKLIEEFDNLEN
                     VLANADKMKGKLQETVQTKADLARLSKTLATIHIDAPVDFDYNHYEVNEFNNDVLLEV
                     FKELEFKTLAATILSYNSKTIGKSTSTTKAASTKKQDSEVYQGSLFDNKDTVEEKETL
                     SEELSNAQFNITNTPHEYKLVATDDDWKALRTEITARKFFCFDTETTSLDPIVAELVG
                     ISISTEAHKAWYISVPEDQQVAQVIANQLKPLWENPEIDKIGQNLKYDISVLKKYGID
                     IKGRLFDTMLIHYLIEPELRHGMDYMAEAYLKYRPIPITELIGEKRERQLSMRDVALE
                     QIKEYASEDADITLQLYNYLVPGLKEIQAYDLYNNIEGKLITVLADLEMAGVNIDAEY
                     LNHYSVELENRIVELERKIYQEAGSEFNINSPRQVGDLLFGTMKIPYRWAKTKQGQHS
                     TDEAKLSELALEYAFVQDILEHRSLSKLKSTYVDALPKMVHHSTGKVHSSFNQALAAT
                     GRLSSNNPNLQNIPIRTREGREIRKAFIPSGQDNILLSADYSQIELRIIAELSKDKNM
                     VEAFQLGHDIHTATASKVYGVAIEEVTPDQRRNAKTVNFSIIYGAGSLNLSRQLGIKR
                     TEAKEIIDQYFLQFNGLKSYMDRMVHEAHEKGYVTTLMGRRRYLRDINSRNAMARSAA
                     ERIAVNSPIQGTAADMIKVAMIGINESFKQAGLSSKMILQVHDELVFDVKKSELDTVR
                     AIVIDKMKNAIPNLSVPIEVSTGTGANWLEAH"
     gene            complement(3516..3986)
                     /locus_tag="PLG24_08860"
     CDS             complement(3516..3986)
                     /locus_tag="PLG24_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HMZ25043.1"
                     /translation="MKAIVNDMFEIDLDSLENQDVSVVVDGDNILARINGVNYRMRLA
                     ESPHNHKKYEIEINNRIYCVRLENDLDQMVNSMGLKGKRTQTVKELKSPMPGLVFRIL
                     KSEGDEVVAGETILILEAMKMENSIKSPGNGRIKSIPVKEGQSIEKGGVLVQFE"
     gene            4458..>5389
                     /locus_tag="PLG24_08865"
     CDS             4458..>5389
                     /locus_tag="PLG24_08865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HMZ25044.1"
                     /translation="MREHLQEDYHRFANALNDVPPVSIRLNQGRHFTIPERSTPCPWE
                     PNAYYLKERPSFTYDPNFHSGAYYVQEASSMIIGKILDMLDKSSWHLIIDLCAAPGGK
                     TTHILDKCPESVFVLANESVKTRLGSLKHNLIKWGRSNCAVISHPVENIAATGLKADL
                     VLVDAPCSGEGLFRKDKESVKHWSEKNLGICQRRQEDILHHIPGMVKEAGYLIYSTCT
                     FNPKENIEQLNKLVKSGYFKSLPINLNPEWGIEEILGKGSIGYQCYPHRVKGEGFFFS
                     LLQRTEKVFIQSNNKKFHQTRNLKKIGKEALNNIP"
BASE COUNT         1803 a         1074 c         1075 g         1437 t
ORIGIN      
        1 tgctttgatc gggaatttcg acaacaatct ctgcatcgtc cgaaaggata atgcactgac
       61 aacccaatcg gctgttgagt ttcggattga tagcccggtc tataaaatct tcctctttgt
      121 cagaaatctc ttccagatca tcctctccct cataaatata cacgtggcaa gtactgcatg
      181 cgcaaacacc tccgcaattg tgattcaggt gaatatcgtt ctcttcagct acttcaagga
      241 tgcttacacc ttcttcgacg tcctcaatag tgacgtcagg ctgcttatct tcaaatttaa
      301 aagttacttt agccatttgt ggaaacaaag atttgcggtt tatttatatc ttttctaaat
      361 taagaaaaaa tcgtattgct aaacaaattc ctaacaggaa agttcattga tggaaatact
      421 gccggatata aaaaaatttt aacaatcata aaagtaaagt ctaccctact gtaggtccca
      481 taccaaaagg ataaatattt tattttcatt ctttttgaaa atcaatatga aaaaaataaa
      541 aatcagaacg ggataccttt aaatgctgtt ccaaaacctc ccaaacgaat aataaattgt
      601 tcaaaattta atgtaagccc cgcacaatac cttactttta cccataaaat taaagttatg
      661 tcttcagaca agaggatttt tttcattgat ggccatgcga tggtatacag agcccattac
      721 gcattcatca acaggccact gatgaattcc aaagggcaaa atgtctctgc aatcaatgga
      781 ttcataagag ctatctggga tttgattgtc aaccaaaaac caacgcatct gggtgttgta
      841 tttgatccaa aaggcggcac attcagaagc gacttatatg aggagtacaa agctcaaaga
      901 gatgcccagc cagaagatat atcatttgct attccataca tcataaagat ccttgaagga
      961 atgaatattc cggtggaaat tattccaaat tatgaagcgg atgatgtcat cggaacattg
     1021 gccaaaaaag cagaaaaaga aggatttgag gtctatatgg tcacgcctga caaggattac
     1081 gcgcaacttg tgtccgaaaa tattttccaa tataagccgg cccgattcgg taatgacata
     1141 gaaatcctgg gtgtagagga ggtgttaaaa aaatgggaaa tcaaacgtgt tgaccaggta
     1201 atagatatcc ttgggttgat gggagatagt gtagataata ttccaggcct cccaggtgtt
     1261 ggtccgaaga ccgccgtcaa attgatcgaa gaatttgaca acctggagaa cgtactggcc
     1321 aatgccgaca agatgaaggg gaaacttcag gaaactgtgc aaaccaaggc agacctggct
     1381 cgactatcca agacactggc aacaattcac attgacgcac cggtagattt cgactacaac
     1441 cactatgaag tgaatgaatt taataatgat gtattgttag aggtctttaa ggaactggag
     1501 tttaaaaccc tggcagccac catattatcc tataattcta aaaccatagg aaagtcaacc
     1561 tcaacaacca aagcagcttc cacaaaaaaa caagattcag aagtatatca agggtccctt
     1621 tttgacaata aagatacagt tgaggaaaag gaaacgctat cggaagaatt atccaatgcc
     1681 caatttaata ttaccaatac accacatgaa tacaagttgg tcgcaacaga tgatgactgg
     1741 aaggcattaa ggacggaaat taccgcaagg aaattctttt gtttcgatac ggaaaccaca
     1801 agtcttgatc caatagttgc tgaattagtg ggtatctcaa tcagcactga agctcataag
     1861 gcatggtata tatcggtgcc tgaagaccaa caagtcgctc aggttatagc aaatcaactg
     1921 aaaccgctgt gggaaaaccc cgaaattgat aaaatcggtc aaaatctcaa atacgatatc
     1981 agtgtcctga aaaaatacgg aatcgacatc aaaggaagac tattcgatac catgcttatt
     2041 cactacctga tcgagccaga actaaggcac ggcatggact atatggcaga agcatatctt
     2101 aaatacagac cgataccaat tactgaattg atcggagaaa aaagagaaag acaactatcc
     2161 atgcgggacg tggctttgga acaaataaag gagtatgcct ctgaggatgc cgatattacc
     2221 ttacagttat ataattatct tgtaccaggc cttaaggaaa ttcaagcata cgatctttat
     2281 aacaacattg agggcaagct gataacggtt ttggccgatc tggaaatggc cggtgtaaac
     2341 atcgatgcag aatatcttaa ccattacagt gtggaattgg aaaacaggat tgtggaactg
     2401 gaacgaaaga tttaccagga agcagggtca gaatttaaca ttaatagtcc gcgacaggtt
     2461 ggtgatctat tgttcggcac catgaaaatt ccttaccggt gggcaaaaac caaacaaggt
     2521 caacattcaa cagatgaagc caaattatcc gaattagcat tagagtatgc atttgtgcag
     2581 gatattctgg agcaccgcag cttaagcaag ctcaagtcta cctatgtcga cgcactccct
     2641 aagatggttc atcattcaac aggaaaggtt cacagcagtt ttaatcaggc acttgctgca
     2701 acaggaagac taagttccaa caatccgaac ttgcagaaca taccgatccg tactcgggaa
     2761 ggccgggaaa tcagaaaggc gttcattcct tccggccagg acaacatact cctttctgca
     2821 gattattctc aaatcgaact tcgcatcata gcagagctca gcaaggataa aaatatggta
     2881 gaggcattcc agcttggaca cgatatacat actgcgactg cttcaaaagt gtacggtgtg
     2941 gccatagaag aggtaacacc tgaccaaaga cgtaatgcca agaccgtaaa tttcagtatc
     3001 atttatggtg ccggctcatt aaatcttagt cgtcaactgg gtataaaacg gacagaagcc
     3061 aaggaaatta ttgatcagta ctttttgcaa tttaatggac ttaaaagcta tatggaccgg
     3121 atggtgcacg aagctcatga aaaaggctat gtaacaacat taatgggtag aaggcgctat
     3181 ttaagagata taaattcaag aaatgccatg gccagaagtg ccgccgaacg aatagcggtc
     3241 aacagtccga ttcaggggac tgctgctgat atgataaaag tggcaatgat cggtatcaat
     3301 gaatcattta aacaagccgg attatcatca aaaatgatac ttcaggtaca tgatgaactg
     3361 gtatttgatg tcaaaaagag tgagttggac actgttcgtg caattgttat tgataaaatg
     3421 aaaaatgcaa ttcctaatct atctgtgcca atagaagtca gtaccggaac cggagctaat
     3481 tggctggagg ctcactgatt ccggtttact tccgattatt caaactgtac caggacacct
     3541 cctttttcaa tggattgtcc ttccttgacc ggaatggatt tgattctgcc gttgccggga
     3601 gactttatag aattttccat tttcattgct tccaggatca ggatggtttc accggccact
     3661 acctcatctc cttccgattt aagaatcctg aacacaaggc cgggcattgg tgacttcaac
     3721 tctttgactg tctgagtgcg cttgccttta aggcccatgg aattaaccat ttgatccaga
     3781 tcattttcca gcctgacaca ataaattctg ttgttgattt caatttcgta ctttttatga
     3841 ttgtggggtg attctgccag cctcattctg tagttcaccc cattgattct ggccaaaata
     3901 ttatcaccat ccacgacgac tgaaacatcc tgattctcca atgagtccaa atctatttcg
     3961 aacatatcat ttacaattgc cttcatcctg ttttttgtac aaagaaacaa aagaaaaagg
     4021 gatatgagaa gaaaaatacg aatcatggaa tcagacatag agaacaaaat gacgggttga
     4081 atgcgggtgg tgggagggtg agagggagaa ttatgaatta ggtagataac taatacaaat
     4141 catccctttc attgtgggat attagaatca aatagataaa attcagaccg tatttcaaga
     4201 tttaaaattt tttcttacct taaaagtatt tataaatcaa tgattgttaa gtattaagca
     4261 aatacctaat tcattcagga acaacaaaat ccaacgacaa atgctaccca cacattgcct
     4321 caatccgggg ctccgtatga gtaaaaatca gcaaaatcta caggttttat aaacaaattg
     4381 acatcagaat ttattatgca ttcattaata ttcaaaaatc gatgccggtt tcattaccag
     4441 aagaatttat aatccgcatg cgcgagcatc ttcaagaaga ttaccatcga tttgccaatg
     4501 ctcttaatga tgtgccgccg gtctcaatca gattgaatca aggacgtcac tttacaattc
     4561 cagaacgatc tacaccttgc ccatgggagc caaatgcata ttaccttaag gaaaggccct
     4621 catttactta tgaccctaac tttcacagtg gtgcttatta tgtccaggaa gcaagtagta
     4681 tgattattgg taagattctt gacatgcttg ataagagtag ctggcatctg atcattgatc
     4741 tttgcgcagc accgggcggt aagactactc acatccttga taaatgccct gaatcagtgt
     4801 ttgttttagc caatgaatcg gttaaaacca gattaggttc attgaaacat aatctgatca
     4861 aatggggcag atctaattgt gcagtgattt ctcacccggt tgaaaacatt gctgcaaccg
     4921 gcctgaaagc agatctggtg ctggtagatg caccatgcag cggagaaggc cttttcagaa
     4981 aagacaaaga gagcgtcaaa cactggtctg aaaaaaatct cggcatttgc caaaggcgcc
     5041 aggaagatat attacaccat attccgggaa tggtaaagga agccggttat ctgatttaca
     5101 gtacctgtac ctttaatcct aaagagaata ttgagcaact gaataaatta gttaaaagcg
     5161 gatattttaa gtcccttccc atcaacctga atccggaatg gggcatagag gaaattttag
     5221 gcaaaggttc catcggatat caatgctatc cacaccgtgt caaaggagaa ggatttttct
     5281 tttcactcct ccaaagaaca gaaaaagtgt ttattcaatc caataataaa aagtttcacc
     5341 aaacaagaaa tctaaaaaaa attggtaaag aagcccttaa taacattcc
//
LOCUS       DAOQQN010000224         5371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6859_length_5371_cov_6.191309, whole genome shotgun sequence.
ACCESSION   DAOQQN010000224 DAOQQN010000000
VERSION     DAOQQN010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6859_length_5371_cov_6.191309"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PLG24_08870"
     CDS             complement(<1..927)
                     /locus_tag="PLG24_08870"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HMZ25045.1"
                     /translation="MFFSNKILICTATVLLCLIFPSLMNSQSLTGKVFENDSKETVVG
                     ATIRIVGYDKGTVSDEYGAYQLYGLKTGDIIIFTSVGLKSRSVTYDGQTTLDIYLERE
                     DKLLDEMVVVGYGSQKRSDISGSVSVIKSDEITSSPALRVEQALQGRASGVLVTQNSG
                     APGAPLTVRIRGTGTINNSDPLFIVDGVPVGGLDFLNPSDIESVNILKDAASAAIYGA
                     RGANGVILITTRSGKHNQEGVINYSSYYGIQNAWRKPRLLNAREYAIISNEAHINAGV
                     KPLPEFADPDALGVGTDWVSAVMGSAPITEHQL"
     gene            complement(1041..2576)
                     /locus_tag="PLG24_08875"
     CDS             complement(1041..2576)
                     /locus_tag="PLG24_08875"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25046.1"
                     /translation="MNRLLFLFFIMILVKSGIVAQDQIILDFETTETTTTFSYFDNGD
                     WPDAQTSVVVNPDKSGINTSDNVLYFVRGVGGQTWAGAYGNPKPTRKIDLTNHNRLKC
                     KVWAEKPGTVQLKLEGGPAGGADNWEKVLPIPATGQWVEMEWDVTLPGDAGEKVPAFG
                     KIYDNIVIFANFLQETTERVYLYFDDFATGEGEAAQPVPVTFVLNAAGYNGNISSASV
                     AGTFNGWDSGANVMAEVSAGSKLYTVTVDILPGVIEYKFVINGNTWEEGLNALDECVT
                     NNNGFINRKASISGETNLIPVCWNKCYNCGEYVSLTVNLGFGAANPPSPDGVWIAGGE
                     KFEAPGGKYRMEDSDGDGVYSINLTRGIGFKSHFTFANGNCADYSCKERLDDQACGDA
                     GNYWDRKLLPVTQDTVVYACFGDCSEVSCLASTKKVLDNPGFMIIPNPASSAVTLKFD
                     ENLRDRGSVLIRNIYGSVVKTVSYTPGMDVIKIEIESLTPGIYMASYVKDGMTGTKLF
                     IKQ"
     gene            2899..>5371
                     /locus_tag="PLG24_08880"
     CDS             2899..>5371
                     /locus_tag="PLG24_08880"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HMZ25047.1"
                     /translation="MTNSFLSFGQNFELGTPPIRNFTKKIYAASPQNWQIGQRSDGMM
                     LFANNLGLLTFTGNSWDLYPIANRTIVRSLLVHPDQRIYIGGQGEFGFFDQKENGRLK
                     YKDLTPLIPQSTRNFADVWQLGYQDKYVLFQTTKEVLIYDGKSVRVYQPPATIKKMFS
                     YRRANYINLSDGSLLKFNGNSFEPVFIAENLPADIVNICDISNGNLRIAGYRSGIFDC
                     VGTKCSLNAFTLNPLYKNLRITVMQRLRDGHIMIGTMDHGLIIVSESGNVLLSLKKEN
                     GLINTSILAAMEDRNGNIWVSTESGIDFIEYKSPFRFLFPDIPYNGTGYSMALFRDRL
                     YLGTNNGLYYTEMLQDRSGPAIFREVAGSKDICWDLDVIDDNLWLGHNEGASIVADDH
                     LLPAFRGKGVWRFIKQDSQNIIFGSYEGFGMITQEGQQYVASILPGFDESSRIVLIDS
                     LFNIWMSHPYRGVYKLKKPHMGSPLSFRKYGLSGGLESDHENYIIKTNTRIYASNDKG
                     IFIYDPKRDQFRRDPTLESLIGYNTGTKLISADEYQNIWFRKGLKIGFLEPYKDEWKV
                     EYRQHILPALPESLAGGFEKVYPLTKDIFLFNCESGFLLFDKRKLIPDLQYTTYLYKC
                     TILSNRDSILLKGVEPNFIPYSKNKPLTLNHDQNSLRFDFSCFSFSDLQVEYKYTLTG
                     SSKSVSAWTSEPFVIYNNLDHGNYQISVQTRIGNVLQKGSIAFHFTIQPAWYNTLWFR
                     GSVLLCMIASIFGLFLLQDKRFKTEKKQLTQKHKRLVSEHADKVAQSEEEVIRLKNEQ
                     LQKDIQFKNNELASLAMHLAHKKDFI"
BASE COUNT         1520 a         1263 c         1082 g         1506 t
ORIGIN      
        1 aagttggtgt tcggttatcg gcgccgaacc cataactgca ctaacccagt ctgtaccgac
       61 acccaaggca tcaggatcag caaactccgg caagggcttg acccctgcat tgatatgtgc
      121 ctcattggat atgattgcat attcgcgggc attgagaagt cggggttttc tccaggcgtt
      181 ttggatacca taatatgatg aatagttgat tactccctcc tgattgtgtt tgccggagcg
      241 tgtggtgatg agaatcacac cattggctcc tctcgctcca tatattgcgg cggatgcagc
      301 atctttaagg atattgactg attcgatatc agacgggttc aggaagtcaa gaccaccaac
      361 cggtacgcca tcgacaataa acaaaggatc actgttgttg atcgtgcctg tacctcttat
      421 tcttacagtt agcggtgcac cgggcgcacc ggaattttga gtcaccagaa cgccggacgc
      481 cctcccctgc aaagcttgtt caacacgaag agccggtgag gatgtgattt cgtctgattt
      541 tattacagat acggatccac ttatatcgct tcgtttttga gatccgtatc ctacgacaac
      601 catttcgtcg agcaacttgt cctctctttc cagataaata tcgagcgtgg tttgcccatc
      661 ataagtaact gatcgggact tcaatcctac cgaggtgaag atgattatgt ctccggtttt
      721 gagtccgtat agctggtagg caccatattc atccgatacc gtacccttgt cgtaaccgac
      781 tatccggatg gtagcaccaa ctacggtttc ttttgaatca ttttcaaata ctttccccgt
      841 taatgattgt gaattcatca acgaagggaa aatcaagcac agcaacactg tggcagtgca
      901 tatcaagatt ttattggaaa aaaacatctg atttttgtaa aaatttaacc cgggggtgcc
      961 gaatggaatc ccctaaaaat ccaaagtatc catgaaaaaa atgcccggca tattcataat
     1021 gccgggcaac atttaatcaa ttattgctta ataaataact tagtacccgt cataccatcc
     1081 ttaacatatg aggccatata tattcccgga gtcaaacttt caatttcaat tttaataacg
     1141 tccattcccg gagtataaga aactgttttt actacacttc cataaatatt acggattaat
     1201 acagaacccc tatcgcgtaa gttctcgtca aattttaggg taacagcact tgaggccggg
     1261 ttgggaatga tcataaagcc cggattgtcc aagaccttct ttgttgaagc aagacaagag
     1321 acttcagaac agtccccgaa gcatgcatat acaacagtat cctgagttac cggcaacaac
     1381 tttctgtccc aatagttacc tgcgtctcca catgcctggt catccagtct ttccttgcag
     1441 gaatagtctg cacagttacc attggcaaaa gtaaaatggg acttgaatcc aatgcctctt
     1501 gtcagattga tagagtaaac accatctccg tcagagtcct ccattcgata tttgcctccc
     1561 ggagcctcaa atttttcgcc accggcaatc caaacgccat ccggcgatgg tggattggcc
     1621 gctccgaagc caagattgac cgtcaggctt acatattctc cacagttata acacttgttc
     1681 cagcatacag gaatcagatt ggtttcccca ctaatggagg ccttccggtt gataaatcca
     1741 ttgttgttgg tgacgcactc atccagcgca ttcaaccctt cttcccaggt atttccgttg
     1801 atgacaaatt tatattctat gacacctggc aatatgtcca ccgtaactgt atataacttg
     1861 gatccggcag aaacttcagc cattacattg gctccggaat cccaaccatt gaaggttccc
     1921 gctacactgg ccgaggaaat attgccattg tatccggctg catttaaaac gaaggtaacg
     1981 ggtaccggtt gtgccgcttc gccttctccg gtagcaaagt catcaaaata caaatagacc
     2041 ctttcggtag tctcctgaag gaaatttgca aagattacaa tgttgtcgta tatcttacca
     2101 aatgccggaa ccttttcgcc tgcatcgccc ggtaatgtga catcccattc catctcaacc
     2161 cattggccgg ttgccggaat tggcaaaact ttttcccagt tgtcggcacc tcctgcaggg
     2221 ccaccctcca acttaagctg aacggtaccc ggtttttctg cccatacttt acacttaagc
     2281 ctattatggt tggtcagatc aatttttctg gtaggtttag gatttccata tgcaccggcc
     2341 catgtttgac caccaacacc acggacaaaa taaagtacat tgtcactggt gtttattccg
     2401 gacttgtcgg gattaacaac cactgaagtc tgtgcatccg gccagtcgcc attatcaaaa
     2461 taactgaacg tcgttgtggt ctcagtcgtt tcaaaatcca gaattatttg gtcttgagcc
     2521 acgatccctg actttactaa gatcatgatg aagaaaagga ataaaagtcg attcataata
     2581 ctaaattttt taagttgata aatgattaaa atgatggaag gctatacgac atatgaaaaa
     2641 atcaccccat aatctcccct gttcgatacg gccgcactgc caaataaaaa ataccgggca
     2701 ccgccttata tcgcaacaaa tataccgaac atgcttaaga attcaaaatt attcactact
     2761 tctttactac gacattgttg tttatttttt acttttactg cttatttttg aaatacggga
     2821 atgcattttt actacgacaa gtactttttt taccctctcc gctacctacc cacccgtatt
     2881 cttttatttt gttttctgat gactaattct tttctctcct ttggccaaaa ttttgagctt
     2941 ggaacaccgc caatcagaaa ttttaccaag aaaatctatg ccgcctctcc ccaaaactgg
     3001 cagatagggc agcgtagtga cggcatgatg ctgtttgcca ataatcttgg gcttcttacc
     3061 ttcaccggca attcctggga cctgtatccc atcgccaacc ggaccatcgt ccgatcacta
     3121 ttggtccacc ctgaccaaag aatatacata ggtgggcagg gagaattcgg cttttttgac
     3181 caaaaagaga atggccggct gaaatacaag gacttaaccc cgttgattcc gcaatctacc
     3241 cgaaattttg cagatgtttg gcaacttggt tatcaggata aatatgttct ctttcagaca
     3301 actaaggagg ttctgattta tgatggtaaa agtgtccgtg tatatcagcc ccctgctaca
     3361 attaaaaaaa tgttttccta tcgccgggct aattatatca acctttctga tggctccctg
     3421 cttaagttca atggtaatag ttttgaaccg gtatttatcg cagaaaatct ccctgctgat
     3481 atcgtaaaca tatgcgatat aagcaatgga aatctgagga ttgcaggtta ccgctccggt
     3541 atttttgact gtgtcggtac aaaatgtagc ctgaatgcat tcaccttgaa tcccttatat
     3601 aaaaatttgc ggattactgt gatgcaacgt cttcgggatg ggcatatcat gataggaacc
     3661 atggaccatg ggctgattat tgtgagtgaa tccggaaatg tccttttgtc tctcaaaaag
     3721 gaaaatggct taatcaatac ttccattctg gctgctatgg aagacaggaa cggcaatatt
     3781 tgggtatcta cggaatccgg aatcgacttt atcgaataca aatccccttt ccgatttcta
     3841 tttcctgata tcccctataa tggcaccgga tactcaatgg ctctgtttcg tgacagatta
     3901 taccttggca ccaacaacgg gctgtattac acagagatgc tgcaggacag gtctggccct
     3961 gccatattca gagaggtcgc cggcagcaag gatatttgtt gggatcttga tgtcattgac
     4021 gacaacctgt ggttggggca taacgaaggg gcttccattg tagctgacga ccatttactg
     4081 cccgcttttc ggggcaaagg tgtgtggcga ttcattaaac aagatagtca aaatataatc
     4141 tttggatcct atgaaggatt tggaatgatc actcaggaag gtcaacaata tgtagcatcc
     4201 atattgccgg gatttgatga gtcaagcagg attgtgctta tagattcctt gttcaatatc
     4261 tggatgtctc atccctatcg gggagtgtac aaactgaaga aaccacatat gggatccccc
     4321 ctttccttcc gtaaatacgg tttgtccggg ggccttgagt ccgaccatga aaattatatc
     4381 attaaaacga atacccgtat atatgcatcc aacgacaaag gaatttttat ctacgacccg
     4441 aagagggatc aattcaggcg cgacccgacc ctcgaaagcc ttatcggata caatacggga
     4501 acaaagctca tctccgctga tgaatatcaa aatatttggt ttagaaaggg tctgaaaata
     4561 ggcttcctgg agccctataa ggatgagtgg aaagtcgaat accggcagca tatccttcct
     4621 gcactgcccg aaagtctggc aggcggcttc gaaaaggtat atcctttgac caaggatatc
     4681 ttcctcttca attgtgagtc gggcttcctt ttattcgata aaagaaaact gattcccgac
     4741 ctccaataca caacatacct gtataaatgt accattctga gcaaccggga ttcaattctg
     4801 ctcaaaggtg tcgaaccaaa tttcattcct tattcgaaaa acaaaccact gactctgaac
     4861 catgatcaga atagcctgag gtttgacttc agctgcttca gcttcagcga cctacaagtg
     4921 gaatacaaat atacgctcac gggaagtagt aagagcgttt cggcctggac atcagaaccc
     4981 tttgtcatct acaataatct ggatcacggc aactatcaaa tctccgttca gactcgcata
     5041 ggaaatgtac ttcaaaaagg cagtatagca ttccatttca ccattcaacc ggcatggtac
     5101 aatacgctgt ggttccgcgg tagcgtgctt ttatgtatga tcgcctccat ttttgggcta
     5161 ttcctgttgc aggacaaaag atttaaaacg gaaaaaaaac aacttaccca aaagcataaa
     5221 aggcttgtca gtgaacacgc tgataaggtc gcacaaagcg aagaagaggt tatccgtctc
     5281 aaaaacgaac aactgcaaaa agatattcaa ttcaaaaaca acgagttggc atctcttgcc
     5341 atgcacctgg cacataaaaa ggattttatc c
//
LOCUS       DAOQQN010000225         5339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_6923_length_5339_cov_5.506813, whole genome shotgun sequence.
ACCESSION   DAOQQN010000225 DAOQQN010000000
VERSION     DAOQQN010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6923_length_5339_cov_5.506813"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1579)
                     /locus_tag="PLG24_08885"
     CDS             complement(20..1579)
                     /locus_tag="PLG24_08885"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ25048.1"
                     /translation="MALYPPPTQEKIYDFVVIGSGMAGLVCATYLTRQGRSVAVLEQN
                     HQVGGCLQIFSREKRIFDTGVHYIGGLGDDQSLMKLFDFLGIREKLHCERLDMDGFDR
                     IIIRQAGVEIPLAQGWDRFQENLTAQFPSERQALQAFVEKIKTTVDRFGPYHLQPADP
                     KLLPTEVFSEGAWDVLNRYFKDDLLKAALGGNSILYSGDRDKTPFYAYALILNSYVEG
                     AYRLVRGGSGLSKALSGLIRIQGGAIVKRCKVTSIGRRDDGVFTLEVADGKHFCAGKV
                     ISAIHPSSLLQLLPEKSVRAGYRHRIETLSNTPALISVHFVVKPEALPYFNNNYYILE
                     TADDAWTIRSLPDEGWPYNYMVSTGCEETGQEWCNTVNVLAYCDYSEFATWEGSFNTV
                     MYPSGRGNDYEAFKNELIHKIKVKLEGQFPGFQEMVTGAYCSTALTYRDYINVPQGSP
                     YGIEKDFNNILSTFVSPKMTIPNLYQTGQNTDLHGVYGASISALLTLSQLEEGKDVFD
                     EIQHFLSTEQG"
     gene            complement(1580..>5339)
                     /locus_tag="PLG24_08890"
     CDS             complement(1580..>5339)
                     /locus_tag="PLG24_08890"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HMZ25049.1"
                     /translation="AGIILYCVLGISRLQWNNDVFSIFPLHASSADYTAQVANMRANN
                     DIMVLLHTESPSDSSLRVLVDNAEWWEHNVNSSSYLSSVIPDAAFRMTDSLQRVLYDS
                     VFFSLPLLLNGNDLDTVRQRLDNAAIERIIARNNRLNSGFSGFAMRHFNQLDPLHLAP
                     IVLKKLETFQTVPGLKQANGFQVSKDERNLFFSFNAIYKDKEDPGYRALNDSLTAMRQ
                     VSEKLFPGVKTYLFGAPIASEINKERSFQDGNLTGSLSILLTLGLLYYFFRRKRITMM
                     VLVPSVVGYGIALATFGFAGVPIVAMAMSSATIILAMGINYSVHLVNARLHSDNIEDA
                     IREIVNPMITGNVTTVAAFALLIMSDSLLLQQFGILASISLAAGVLTALLVLPQWLVQ
                     TNTTKAEVSIPWFGRLANYEFHKNKWIIGLVIIGSIAMFFPASRVQFRGDLTDLNYIP
                     REDRAGYNILENDLGFNLSRTNLRVNAPAPDSALALYNEARRDLLQIDSTALIPDLSA
                     IRPTATEQYNNQAGWSELWADSLLSEKKFFAASLHGGQDVVRFLDRVKTFDTIAVPER
                     LTEEFSRTILSKFMKNEADGSVSLNVPVTGHFDLGNQDSDKPYHLFNGRYFATQTAKY
                     LQQDFNNILAYSAIIVFLLLFLVYGRLELAILAFIPMTLSWFWILGIAYLVGIQIHIV
                     NLILCTFIFGLCDDYSIFMIDGLTKEYATGKKVINKDKKVIIISVMVTMIGLGALLAG
                     RHPAFHSFAILALIGLAVVLILSLTIQPALYHYFVTYRTNKGNAPVTILSFCVSIITF
                     STFIALGLILSIVGMILKWTGLMRLAFIRYTFHLLVRISCHFVIWITPTAHHVKEDMH
                     YIDWKKPGIIIANHQTHIDLLIMIGLHTKIVVLTNEWVYNNPVYGGLVRAAGYLPGFS
                     GMEHLLEQARKQIDLGYFIVIFPEGTRSESGEIKRFHKGAFFLAEQLNVPIYPVLLHG
                     LNLGLTKGDQNIKKWSSVIRALPAVAPNDGRFSPEYSLRGKEINHWMRREFEKIKEEK
                     EVPSYYFDTLRKNYLYKGPVLYWYAKIKMLGEKLYSHLHELIPRQATILDLGCGYGMM
                     DYMLAMCSSGRAITGVDYDEEKVAVAANNFYAKRLNIRFESGDIRHYAITNYDVFLLY
                     DSLHYLKKEEQTALLDKCAQCLNPGGMIIIREGVSGSDKRRHFNTRLTERFSTKILRF
                     NKTTGSLKFIDEEDLYELARRFNFTVEKRKDSRISSNTLFIIRNQRPV"
BASE COUNT         1441 a         1264 c         1221 g         1413 t
ORIGIN      
        1 aacagtctca atgccttctt catccttgtt cagtgcttaa gaaatgttgg atttcatcaa
       61 aaacatcctt gccttcctcc agttgagaca aggtcagcaa ggcagatatg gaggcgccat
      121 agacgccatg caggtctgta ttctggccgg tctggtatag attgggtatc gtcattttgg
      181 gtgaaacaaa ggtagacagg atattgttga aatccttctc tatgccataa ggcgaaccct
      241 gaggcacatt gatatagtcg cggtaagtca gtgctgtcga gcaatacgcg ccggtcacca
      301 tctcctggaa tccgggaaat tgtccttcca gttttacctt gatcttgtgg atcaattcat
      361 ttttgaaagc ttcgtaatca tttcctcgcc cggaaggata cataacagtg ttgaaacttc
      421 cctcccaggt ggcaaattcg gaatagtcgc agtatgcgag gacattcact gtattgcacc
      481 actcctgacc tgtctcttca cagccggtgc tcaccatgta attgtagggc cagccttcat
      541 cgggaagtga ccgtatcgtc caggcatcat ctgcagtctc aaggatgtaa tagttattgt
      601 tgaagtaagg cagagcctcc ggctttacga caaaatgcac cgatatcagt gcaggtgtat
      661 tgcttagtgt ctcgattcgg tgccgatatc cggcccgtac acttttttca gggagcaatt
      721 gcaacaagga cgaaggatga atggccgaaa tcaccttgcc ggcgcagaag tgctttccat
      781 cagctacttc cagggtaaat acaccgtcat cacgcctgcc gattgaggta actttacacc
      841 ttttgacgat ggcccctccc tgaatacgga tcaatcctga caacgccttc gagagacctg
      901 agcctccacg gaccaatcga taggcacctt ctacataact gttgaggatg agggcgtaag
      961 cgtagaatgg tgtcttatca cgatctccgc tataaagaat ggaattgccc cccagggcgg
     1021 ctttgagcag gtcatcctta aaatatcggt tcagtacatc ccatgcacct tccgaaaata
     1081 cttcagtagg taataatttc ggatcggctg gttgtaaatg atagggacca aacctgtcaa
     1141 cagtggtctt tattttttca acaaaggctt gcaatgcctg tctttctgaa ggaaattgag
     1201 ccgtcagatt ttcttgaaac cgatcccagc cctgggccaa cggtatttca acgcctgcct
     1261 gcctgatgat gatccggtca aagccatcca tatccaaccg ctcgcagtgt agtttttccc
     1321 gaatgcctaa aaaatcaaac agcttcatca gggattgatc atcaccaagg ccgccaatat
     1381 aatggacacc ggtatcaaat atccttttct cccgggaaaa tatctggaga catcctccta
     1441 cctgatgatt ctgctccagt acggccactg atcgaccctg ccttgtaaga tatgtcgcac
     1501 acaccaatcc ggccatgccg ctcccaatga cgacaaagtc atatattttt tcttgagtcg
     1561 gaggtggata caaggccaat cagacaggtc gttgatttct gatgatgaat agcgtattgg
     1621 atgaaatccg cgaatctttt cttttctcta cggtaaaatt aaaccttcgt gccaattcat
     1681 acaaatcttc ttcatcgata aatttcaggc tgccggtggt cttgttgaac ctcaaaatct
     1741 tcgtcgaaaa ccgttctgtc agccgggtat tgaaatgacg ccgcttgtct gaaccgctaa
     1801 ccccttccct gatgataatc ataccaccgg gattgagaca ctgagcgcat ttgtccaaaa
     1861 gggcggtttg ttcttccttt ttcagatagt gcaggctgtc atacaacaaa aacacatcgt
     1921 aattggtgat ggcgtaatga cgtatatcac cactttcgaa gcgtatattc aatctcttgg
     1981 cataaaaatt gttggcagca acggccacct tttcttcatc gtaatccact ccagtaatgg
     2041 cccttccact gctgcacatg gccagcatat agtccatcat gccgtagcca cagccaaggt
     2101 caaggatggt ggcctgcctc ggtatcaatt catgtaaatg tgaataaagc ttttcgccca
     2161 acatctttat cttggcatac cagtatagca ccgggccttt atataaataa tttttacgca
     2221 gggtatcaaa ataatacgaa ggaacttcct tctcctcctt tattttctca aattcccggc
     2281 gcatccagtg gttgatctcc ttaccgcgta atgaatattc cggactgaat cgtccgtcat
     2341 tgggagctac agcagggagg gcccgaatga cagaactcca tttctttatg ttttgatcac
     2401 cttttgttag gcctaagttg agaccatgaa gcaatacagg atagattggc acattcaatt
     2461 gctctgccag aaaaaaagct cctttgtgaa accgcttgat ctctccactt tcggaacgtg
     2521 taccctccgg gaaaatgacg atgaaatacc caagatctat ctgcttacgc gcttgttcca
     2581 acaaatgctc catccctgaa aatcccggca gataacctgc tgcacgaacc aatccgccat
     2641 atacaggatt attgtacacc cactcattcg tcaaaacaac gattttggta tggaggccta
     2701 tcattatgag caggtcgatg tgtgtctggt gattggcaat aataatcccg ggttttttcc
     2761 agtcgatata gtgcatatct tccttgacat gatgggctgt cggagtaatc caaatcacga
     2821 aatggcacga tattcttaca agaaggtgga aagtatatcg gataaaagca agccgcatca
     2881 atccagtcca tttcagaatc attccgacta tgctcaatat taatccgagt gcaatgaagg
     2941 ttgaaaaggt gatgatgctg acacaaaaac tcaaaattgt tactggggca ttgcccttat
     3001 tggttctgta ggtgacaaag tagtgatata atgcgggttg aatggtcaat gaaagtatca
     3061 atacaacggc cagtcctatc aatgccagta tggcaaagga atggaaagcg ggatgcctgc
     3121 ctgccagcaa tgcaccaagt cctatcattg ttaccattac ggatatgatg attaccttct
     3181 tgtctttgtt gattaccttc ttccctgtcg catattcttt cgtgagccca tcgatcataa
     3241 atatcgaata atcatcgcat agaccgaata tgaaggtgca caatatcagg ttgacgatgt
     3301 ggatctgtat gccgaccaga tatgcgatgc caagaatcca gaaccagctc agtgtcatgg
     3361 gtatgaaggc aagtatagca agctcaaggc ggccatacac cagaaataac agtaaaaaga
     3421 caatgatggc actatatgca aggatgttat taaagtcctg ttgcaggtat ttggctgttt
     3481 gggtggcgaa gtagcgacca ttgaagaggt gatatggctt atcactgtcc tgattgccta
     3541 aatcaaaatg acccgtcacg ggaacattga gtgatacact tccatctgct tcatttttca
     3601 tgaatttgga gaggatggtc ctggaaaact cttctgtaag tcgctcagga acggcaatgg
     3661 tgtcaaatgt tttgacccgg tccagaaagc ggacgacatc ctgcccgcca tgtaaggatg
     3721 ccgcaaaaaa tttcttttca gacaacaacg aatccgccca aagttcagac catcctgcct
     3781 ggttgttgta ctgttcagtg gcagtaggcc gaatagctga taaatccgga atcagggctg
     3841 tactgtctat ctgaagcaaa tcccggcgag cctcattgta cagggcaagg gcagaatccg
     3901 gcgctggagc attgacgcgg agattggtcc gtgacaggtt gaatccgagg tcgttttcca
     3961 gaatattata accggctcta tcttcccgtg gtatataatt caagtcggta agatcgcccc
     4021 ggaattgaac cctggatgcc ggaaagaaca tggcaatgga gccaataatc accaatccga
     4081 tgatccattt gttcttgtga aattcataat tagctaatct cccaaaccat ggaatagata
     4141 cttctgcttt cgttgtattt gtttggacaa gccattgggg caataccaac agcgctgtca
     4201 acacacctgc agcaagactg atggaggcca ggatgccaaa ttgctgcaat aacagactgt
     4261 ccgacattat caacagggca aaggccgcaa ccgtcgtcac attaccggtg atcatcgggt
     4321 tgacaatctc ccggatggca tcttcgatgt tgtcactgtg cagtcgggca ttgaccagat
     4381 ggacggaata attgatgccc atagcgagga tgatggtagc ggaggacatg gccatcgcaa
     4441 caatcggcac acctgcaaag ccaaatgtcg ctaatgcaat accatagcca actacggagg
     4501 gtactaaaac catcatagtt atacgcttcc tcctgaaaaa gtaatacaaa agtcccaggg
     4561 tcaaaagaat ggataggctg ccggtcaggt ttccatcctg aaaggacctt tccttgttta
     4621 tttcggaagc gataggagct ccaaacaggt aggtcttcac ccccggaaac agtttttcgc
     4681 tcacttgccg catggctgtt aagctgtcgt tgagagccct gtatccggga tcctccttat
     4741 ccttgtagat ggcgttgaag ctgaaaaaca ggttgcgttc gtcctttgat acctgaaacc
     4801 cattggcttg tttcagaccg ggtactgttt ggaatgtctc taatttcttt aggacaatgg
     4861 gtgccagatg taagggatcc aattggttga agtgcctcat tgcgaagccg ctgaatccac
     4921 tgttcagtcg gttattacgg gcgatgatgc gttcgatggc ggcattgtcc agtcgttgtc
     4981 ttacggtgtc aaggtcattg ccattgagca agagtgggag actgaaaaac acgctgtcgt
     5041 aaagcacacg ttgcaggctg tccgtcatcc ggaaagctgc atcggggatt acagaagaca
     5101 gataggaaga agagttcacg ttgtgttccc accattctgc attgtccacc aatacacgaa
     5161 gggacgagtc gctcggcgat tcggtgtgca acaggaccat gatgtcgttg ttggcgcgca
     5221 tattggccac ctgtgcagta taatcggcag atgacgcatg tagcgggaag atggagaaaa
     5281 catcattatt ccattgcaac cggctgattc ctaagacaca atacagtatg atacccgcc
//
LOCUS       DAOQQN010000226         5272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7063_length_5272_cov_6.285413, whole genome shotgun sequence.
ACCESSION   DAOQQN010000226 DAOQQN010000000
VERSION     DAOQQN010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7063_length_5272_cov_6.285413"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLG24_08895"
     CDS             <1..224
                     /locus_tag="PLG24_08895"
                     /inference="COORDINATES: protein motif:HMM:NF023298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3390 domain-containing protein"
                     /protein_id="HMZ25050.1"
                     /translation="TTSKTIVMKGMRTVLSSPMIYHTAAKAGRAILSNAPFLIGNRFN
                     PWFQGREMPKPPKQSFGEWYRQNRAKDGE"
     gene            214..816
                     /locus_tag="PLG24_08900"
     CDS             214..816
                     /locus_tag="PLG24_08900"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HMZ25051.1"
                     /translation="MENNARNIILKAVNLANEGLVRPDLPDPHIDSTTYDDAVTRFIT
                     VLEDIGGKAVRVATMEEVHTGVASMFDLSGMRVFSAGFEQGRFELPLETSIGPRRYSD
                     IGLLITTSDLGVAENGAIWLDESKVPDRILPFIAENLVVILSGDAIVHNMHHAYDKLG
                     EAAVGFGVFIAGPSKTADIEQSLVLGAHGARTMTVFIVGA"
     gene            1067..2023
                     /locus_tag="PLG24_08905"
     CDS             1067..2023
                     /locus_tag="PLG24_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25052.1"
                     /translation="MRAYFLFPIFLTLISCSTSNNKAFINNVQKSSCNQENSYAYTKS
                     DIPIPLQELKIDSSLFMYFNERDLNVANAIGVIDVLSDYIQKKGRNRDAASPEQKIEW
                     IQIRQELNDRIDLASLEISAVTAELDCEEERLNQIASFLNEKEKRKESNLTVTSIILG
                     AVSAIVTGVLVSKKDQSNTADIMGVGVGIADVTLGILLLTSSRKITLEHKRNVLRELW
                     LGTETSTTYPPSIWYYLNYNPVPGRISLRERLISNWKSFGQIRKTENNPDGDLDIYFG
                     DGGKYTIDELENRANMYDQIESQIKLMKQDLRNLKESIISVK"
     gene            complement(2091..3644)
                     /locus_tag="PLG24_08910"
     CDS             complement(2091..3644)
                     /locus_tag="PLG24_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HMZ25053.1"
                     /translation="MTRYTSLFIFILLAFITVSGLSCKSEVKKAIEPPKPAELFNRYK
                     ESVVLIKTSFYARLVLDNGLTLYFSNPDNQEGDPFTEDENEARQNPLTIFGTGFFLTR
                     DGVIATNRHVAYPILEDLDVLGMLKQRYDALKSFIIHEIEINNDTLNMIDNALNQNPT
                     DSQIDILKEKAEEIVNRNIFYRIYLASNDFDPKQSSLEIIPVKVEIAYNNSFADENSG
                     YSACNILGKSEDKEIDLAILQLKDRHTPRKVTTLFDFENHDPNIENGTADSTDTYDLN
                     NSLEINKKLYMIGYNHGLTVATTSDGLKAQFTQGSVSQESDKYRVLYSIPALQGSSGS
                     PIIDRWGNLVAVNFAMVAGTQSFNYGIMAKHLKNLLHYYHNGNSTHSFDFGTTSLESQ
                     NNQNVAVSNDENSRPDFRTIIHNFLLAEDKRDFSAIYPFFSNNIRRYWDMTNPTETDL
                     RTRYGKLWRKSIHSQNNITKIEKINFYTYDVSTHFSFTDIDNNYKEVYATFRIVFDDT
                     GKITEIFGK"
     gene            complement(3905..5188)
                     /gene="purD"
                     /locus_tag="PLG24_08915"
     CDS             complement(3905..5188)
                     /gene="purD"
                     /locus_tag="PLG24_08915"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HMZ25054.1"
                     /translation="MVPYNILILGSGGREHAIAAHTVKSPLCNRLFIAPGNAGTAKVG
                     QNLPFAATDFIALKKNILEQDIRMVIIGPEDPLAHGIADKIHADSELQHVLVFGPGAE
                     GAQLEGSKAFAKRFMMKAGIPTASYREFSKDQLDETIEYLRSHPLPIVIKADGLAAGK
                     GVIIAPNVETGIDAVREIFGGQFGTSGDRIVIESFLDGIEFSVFIATNGEEYVLLPVA
                     KDYKKIFDEDKGPNTGGMGAVSPVPFVDNTMMIKVTEKIIRPTLAQLKQDNIPYTGFI
                     FFGLIAVGGDPYVIEYNVRMGDPETEVVFPRIKSDMIEIMEKLILHKPLPEMIIESDY
                     AVGVYLVSNGYPGDYEKGKAIDVDSLPEYCTLYHGGTKQDESGTLVSNGGRVIFSCAK
                     DSSLTSARRKALEGADSIVMDGKFYRRDIGLDLDH"
BASE COUNT         1511 a         1030 c         1198 g         1533 t
ORIGIN      
        1 ccaccacatc caagactatt gtcatgaaag gaatgcgcac ggtgttgagt tctccgatga
       61 tttatcacac agccgcaaaa gccggcagag caatattgag caatgcgcca tttctgatcg
      121 ggaaccgttt caacccatgg ttccagggaa gggagatgcc gaaacctcca aaacagtcgt
      181 ttggcgaatg gtatagacaa aaccgtgcaa aagatggaga ataacgctcg caatataata
      241 ttgaaagcgg tgaatcttgc caatgaaggc ctggtaaggc cggacttacc ggatccgcat
      301 attgacagca caacgtatga cgatgcggtg acgcgtttta taactgtcct ggaagatatt
      361 ggtggtaagg cggtcagagt ggctacgatg gaagaggtgc atacaggagt tgcatccatg
      421 tttgatctgt ccgggatgcg tgttttttca gccggctttg aacaaggccg tttcgaattg
      481 cctttagaaa caagcatcgg gccaagaagg tatagtgaca ttgggctgtt aatcaccact
      541 tccgacttag gtgttgcgga gaatggcgct atttggctgg atgaaagcaa ggtgcctgac
      601 agaatattgc cattcatagc tgaaaatctg gttgttatct tatccgggga tgccatcgta
      661 cacaatatgc accatgcgta tgataagctg ggtgaagcgg cagttggatt tggtgtattt
      721 attgcaggac catcgaaaac tgctgatatt gagcaatcgc tggtgttggg agctcacgga
      781 gccaggacga tgactgtttt cattgtggga gcatgaaagg gattggaatt atagatctgt
      841 atttgtatca agaaattccc tcaatggtag agtacgaccg aatgtcgcat tcgaataaca
      901 ttgaagatat agccgtaatg tatgatgaaa aagtggctgg atagtgattt tcagggagtt
      961 tgacgctgat tatagtatgt agaatcatga ccttataaat aaaacatcta taatcggacc
     1021 taattcattg tgaagtagtg tattttgcaa caaaaataca ggttttatga gagcttattt
     1081 tctatttcca atttttctca cacttatttc ctgctcgaca tcaaataata aagcatttat
     1141 caacaatgtt cagaaaagtt cttgtaacca ggagaatagt tatgcttata ccaagagcga
     1201 tattccaatc ccccttcaag agctgaaaat agattcatcg cttttcatgt atttcaatga
     1261 aagggattta aatgtcgcga atgccatcgg tgtgattgat gtattgagtg attatattca
     1321 aaaaaagggc agaaataggg atgctgcctc tccggagcaa aagatcgaat ggatacagat
     1381 ccggcaggaa ctgaatgacc gaatagacct ggcttcgctg gagatatcag cagtaactgc
     1441 tgagttggat tgtgaagaag agcgcttgaa tcagatagct tcttttttaa atgaaaagga
     1501 gaagcgaaag gaatcgaacc tgacagtaac atccatcatt cttggtgctg tcagtgcaat
     1561 tgttaccgga gtcctggtgt caaaaaagga tcaaagcaac actgccgata ttatgggcgt
     1621 cggagtgggt attgcagatg ttacactcgg aatactactt ttgacaagca gtcgcaagat
     1681 tactctggaa cataaaagaa atgtcttacg tgagttatgg ctgggtactg aaacctcgac
     1741 tacttatccg ccctcgattt ggtattatct caattacaat ccggtacccg gcaggatctc
     1801 tttaagggag cgattgatca gtaactggaa aagttttggt cagattcgga aaacggaaaa
     1861 taatccggat ggggatctgg atatttattt cggtgacggt ggaaaataca caattgacga
     1921 actcgagaac agagccaaca tgtacgatca gatcgaatcg caaattaaat taatgaaaca
     1981 ggacctccga aatctgaagg aaagtatcat ttctgtgaag tagaacaggt gtgtgaaaaa
     2041 actatttgta atgatataaa cccttcaacg ccctatataa cagtcacgcc ttatttgcca
     2101 aatatctcgg taatttttcc ggtatcatca aaaacgatgc gaaaggtggc atatacttcc
     2161 ttgtaattgt tgtcaatatc tgtaaatgaa aaatgtgtgc tgacatcgta ggtgtaaaaa
     2221 ttgatctttt caatttttgt gatgttgttt tgagaatgga tagattttct ccataacttt
     2281 ccgtatctgg ttctcaagtc agtctctgtc ggattggtca tgtcccaata acgtctgata
     2341 ttatttgaaa agaaaggata tatggcggaa aaatctcttt tatcttcagc tagaaggaag
     2401 ttgtggatga tggtccggaa gtcaggcctt gaattttcat cattagatac cgctacattt
     2461 tgattattct gactttccaa ggatgtagtg ccaaaatcga aagaatgggt tgagttcccg
     2521 ttgtgataat aatgcaaaag attttttagg tgctttgcca taatgccata gttgaaactt
     2581 tgtgtgcccg caaccattgc gaagttaaca gcaacaagat tgccccatcg atcgataatc
     2641 gggcttccgc ttgagccctg caaggcagga atggaataaa ggacacgata tttgtcagat
     2701 tcttgactta ccgagccttg agtaaactgt gctttgaggc catcggaggt agtggcaacg
     2761 gtaaggccgt gattgtagcc aatcatgtat agctttttat taatttccag actattgttc
     2821 aggtcataag tatcagtaga atccgccgtt ccattttcga tattaggatc gtgattttca
     2881 aaatcaaata gcgtagtgac tttccggggg gtatgcctgt ctttgagttg aaggatggcg
     2941 aggtcgattt ctttgtcttc cgatttgccc agaatattac aggccgaata tcccgaattt
     3001 tcatctgcaa aactgttgtt ataggcgatt tcaaccttta ccgggataat ttcaaggctg
     3061 gattgttttg ggtcaaaatc attagaagcc agataaatcc tgtaaaatat atttcgattt
     3121 acaatttctt cggctttctc tttcaaaatg tctatttgtg aatcggtagg attttgattg
     3181 agggcattgt caatcatgtt cagggtgtca ttattgattt cgatttcgtg aatgataaat
     3241 gatttgagcg catcgtatcg ttgcttgagc atgcccagca cgtcaaggtc ttccagaatg
     3301 ggatatgcca catggcgatt ggtggctatc acaccatccc ttgtaagaaa aaagcctgtg
     3361 ccaaaaatgg tcaacggatt ttgtcttgcc tcattttcat cttcagtaaa tgggtcccct
     3421 tcttgattgt ccggatttga gaaatatagg gtgaggccat tgtcaagtac caaccttgca
     3481 taaaaacttg tttttatcag cacaacactc tccttatacc ggttgaataa ttcagccggt
     3541 ttaggcggct cgatggcttt ctttacctct gatttacagg ataatccgga tacggtgatg
     3601 aaagcaagta aaataaatat aaatagcgaa gtgtaacgtg tcatatcatg ttcgactgat
     3661 tttgggataa taacttgtgc aaaagcattg atccaaagac ctgaattaat aaacctaaaa
     3721 tccggtaaaa aaattcaatc cggagcgaat agactaatac ttttgactca aaacagtaag
     3781 aattattaga cctacatgat ccgggtggag ttattggttc ggtcatttgt accgtaaact
     3841 actaatcgaa atttaccctg atcgcaaatt taccgatggt tccataaagg tgatttattg
     3901 actatcaatg gtccagatca agcccgatgt ctcttctgta gaattttcca tccataacga
     3961 tagaatctgc tccttccaaa gctttcctgc gtgcactagt cagactggaa tcttttgcac
     4021 acgagaaaat cacccggcca ccgttcgaca cgagtgtacc cgattcatcc tgcttagtac
     4081 ctccatggta cagggtgcag tattcgggaa gagaatcaac atcgatggcc ttacctttct
     4141 cataatctcc gggataaccg tttgacacta aatatacacc tacggcgtag tccgattcta
     4201 ttatcatttc gggcaagggc ttatgtagga taagtttttc catgatctct atcatatcac
     4261 ttttgatgcg aggaaagacg acttcggtct caggatcccc catcctcacg ttatattcga
     4321 tgacgtaagg atctccaccg acagcgataa gtccaaagaa gatgaagccg gtatagggaa
     4381 tgttatcctg cttcagctgg gcaagggtag gccggataat cttttcagtg accttaatca
     4441 tcatcgtatt gtcaacaaat ggaaccgggc tgacggctcc catgcctccc gtattgggac
     4501 ctttgtcttc atcaaagatt tttttgtagt cttttgctac cggaagcagg acatattctt
     4561 caccattggt ggctataaat actgaaaatt caatgccgtc tagaaaggat tcgatgacga
     4621 tcctatctcc ggaggttcca aactgtccac cgaaaatctc tcgtacggca tcgatgccgg
     4681 tctcaacatt cggtgctatg atgacgcctt tacctgcagc caggccatct gccttgataa
     4741 cgatgggtaa tggatgcgag cgaagatatt caatggtttc gtccaattgg tccttggaaa
     4801 actcccgata cgatgccgta ggaatgccgg ccttcatcat aaagcgcttg gcaaaagcct
     4861 tggaaccttc cagttgtgcg ccttctgcgc ccggtccgaa gacaagtaca tgttgcaatt
     4921 ctgaatctgc gtgaatcttg tctgcaatgc catgtgcaag cggatcttca ggcccgatga
     4981 taaccattcg gatgtcttgt tcgagaatat ttttcttcaa cgcgatgaaa tcagttgctg
     5041 caaagggcag attttggccc actttagctg ttccggcatt tccgggcgct atgaagagac
     5101 gattgcataa agggcttttc actgtgtgcg cggctatggc gtgctctctt ccaccacttc
     5161 ccaggatcag aatattatat gggaccattg attaaagtag gtttgtcaag atgcaaaaat
     5221 aggaaaggtt gataactata atggataaag ggaaaatgaa aattatttgt tt
//
LOCUS       DAOQQN010000227         5265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7076_length_5265_cov_5.460269, whole genome shotgun sequence.
ACCESSION   DAOQQN010000227 DAOQQN010000000
VERSION     DAOQQN010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7076_length_5265_cov_5.460269"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="PLG24_08920"
     CDS             <1..1031
                     /locus_tag="PLG24_08920"
                     /inference="COORDINATES: protein motif:HMM:NF002557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HMZ25055.1"
                     /translation="TNNDYNENGLGDLLVEKGDSYDINLEVFYALRDSITGWPGGKPG
                     NQLPNHPERSHIIPKRCLIFSPHPDDDIISMGGTFMRLHDHGHEVHVAYQTSGNIAVT
                     DEFVTRFLDFAVGFEQMFGIDNARSEQILNESRSFLQNKKPSQSDTEEVRAIKGLIRR
                     GEATATCRYVGLPEGRWHFQNLPFYETGTIVKNPMGEKDIELTMKLLREIKPHQVFAA
                     GDLADPHGTHKVCLDIVLESLKRIKEAGDEWIKDCWLWLYKGAWQEWDIGEIMMAVPM
                     SPDQVLKKRHGIFIHQSQKDMVPFQGSDAREFWQRAEERNANTANLYGALGLTRYAAI
                     EAFVRWDY"
     gene            complement(1106..1261)
                     /locus_tag="PLG24_08925"
     CDS             complement(1106..1261)
                     /locus_tag="PLG24_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25056.1"
                     /translation="MKSFVRLFVPKNYYRLTILQVNRLSWHCEAAFTEAGLMSGVDFE
                     TPTSPKD"
     gene            complement(1420..2130)
                     /locus_tag="PLG24_08930"
     CDS             complement(1420..2130)
                     /locus_tag="PLG24_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ25057.1"
                     /translation="MGTNGRKILLVEDDQNFGDVLKSYLEMNDYEVSLGKDGVSGMEL
                     YRGSKYDLAIFDIMMPRKDGLTLAKEIRERDKDLPIIFLTAKNMKEDILHGFRAGADD
                     YITKPFSSEELLLRIQALLKRSAPKSDPEDEVKDFVIGKYKFNFPLRILTYTPDPTDE
                     EKLSPKEAMLLKMFCKRQNNVLERSEALTKIWGEDNYFTARSMDVFITKLRKYLKKDE
                     NIEIVNIHGNGFQLLVKE"
     gene            complement(2163..3530)
                     /locus_tag="PLG24_08935"
     CDS             complement(2163..3530)
                     /locus_tag="PLG24_08935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMZ25058.1"
                     /translation="MKKSTIWILVTLMSIALFGLTGIQYYWISSAVKTAEEKFQTDVF
                     DVLNKVADQLRNDERYRMYQETFGSRSGSRSMATEFEYESLRRMFNETSLGERIKVKQ
                     LNYYIKTFLADKNIREEYDYGVYSQTAKSFVILNGYYAVTNNNKNVYHDNMDQGLFNT
                     PYKVSIFNDSGSLEHGELMIYFPHRSSFRNLWGIVLSSALFTAIILFTFIFTIYEIVT
                     QKKLSIMKNDFLNNMTHEFKTPIATISLATDSISNDNIINSPDKVRRFLDIIKQENNR
                     MLSQVEKVLQMALIDRKKMKLNLSEIHLHDVIKSAVTHINLQVEQKGGYIETFDEADP
                     DIIQGDITHVSNMVHNLLDNANKYSPEYPEITVRTRNVADGIQVIVTDKGIGMNKEQR
                     KHIFDKFYRATSGNLHDVKGFGLGLSYVKAMITAHKGTVDVKSELGKGSTFTLYFPYK
                     QQIED"
     gene            3693..>5265
                     /locus_tag="PLG24_08940"
     CDS             3693..>5265
                     /locus_tag="PLG24_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HMZ25059.1"
                     /translation="MNYLTLEHIKKSYGDKVLFDGLSMSINEGHKIALVARNGTGKTT
                     LLQVIAGLEGSEGENAKILFKKGLRIEYLTQDPSFNPEHTVIDAVFDSDNQTLIAVKE
                     YERALLTPGNEARLQAAVNDMEDKKAWDVEARVKEILTKLKIGNFDQKVNQLSGGQKK
                     RLALAKVLISEPELLILDEPTNHLDMEMIEWLEEYLQHPGLTLLLVTHDRYFLENVCN
                     QILELENGNLYKYSGNYSDYLEKKHAKAENEAIVLDKNKKLFLKELEWVRRMPQARTT
                     KAKARIDKFDELKNELSQQREAAILQINIKSNRLGSKIIEAHNVGKSYNDRHLFSHFN
                     YKFNRFDRVGIIGPNGSGKSTFLNILTGNLKPDEGKIVIGDTVVFGYYTQSGINLKED
                     KRVIDVITDVAEYIPTEKGHNITAASLLERFMFPREQQQVYVSQLSGGEKRRLYLLTI
                     LMANPNFLILDEPTNDLDLVTLNVLEDFLTTYEGCVVLVSHDRYFMDKLVDHLFVFGK
                     DETIIDYPGNYSQYRA"
BASE COUNT         1494 a         1190 c         1141 g         1440 t
ORIGIN      
        1 ttaccaacaa cgattataat gagaatggtc taggcgatct gctcgtggag aaaggagatt
       61 cttacgacat caacctggaa gtattttacg ctctgcggga ttccatcacc ggctggccgg
      121 gaggtaagcc gggcaatcag ttgcctaacc atccggagag aagtcatata atccccaaac
      181 gttgcctgat cttctccccg catccggatg acgatatcat cagcatggga ggtaccttca
      241 tgcgactgca cgaccatggt catgaagtac atgtcgccta tcagaccagt ggcaatattg
      301 cggttacaga tgagtttgta acccgatttc tggattttgc ggttggattt gaacagatgt
      361 tcggtattga caatgcccgt tctgagcaga tactcaacga atctcgttcc ttcctgcaga
      421 acaagaaacc tagccagtcc gatactgaag aggttagggc catcaagggt ctgatccgac
      481 gcggtgaagc tacggcaacc tgtcgttatg taggactgcc tgaagggcga tggcactttc
      541 aaaacctgcc attctatgag accgggacca tcgttaaaaa cccgatgggc gaaaaggata
      601 tcgaactcac catgaaactg ttgcgtgaga taaagccgca tcaggtattt gcagccggag
      661 accttgccga tccccatggc acgcacaagg tgtgtttaga catcgtattg gagagtctca
      721 aaaggataaa ggaagccgga gatgaatgga ttaaggactg ctggctatgg ctatacaagg
      781 gagcatggca ggaatgggat atcggggaga tcatgatggc tgtacctatg agcccggatc
      841 aggtgctcaa aaaaaggcac ggtatcttca tacatcaatc ccaaaaggat atggtgccgt
      901 tccagggcag tgacgcaaga gaattttggc agagagccga agagcgaaat gcgaatacgg
      961 caaaccttta tggtgctttg ggacttaccc gatatgccgc catcgaggca ttcgtacgtt
     1021 gggattattg atcagaaaaa tgtacctgta acaatttaca ccggtagttt ctgatcatgc
     1081 tatttggcac caatctccgg gagaattaat ctttcgggga tgtaggagtt tcaaaatcaa
     1141 ctccgctcat cagcccggct tcggtaaaag ccgcttcaca atgccacgac aacctgttga
     1201 cctgtaaaat ggttaacctg taataatttt taggtacaaa aagccggaca aaacttttca
     1261 tttgtccacc atttagtgct gaggaaatat tcaggattga gtcattgtca gaccgcgaaa
     1321 tatccaagtt attatggcta tttggctggt gaaagtaaaa ttcctatcca ttcttacgaa
     1381 aaaatgtttg actatctttt ttgaataggc aaataaatac tattctttca caagcaactg
     1441 aaatccattg ccgtggatgt tgacgatttc gatattttca tcctttttca aatacttacg
     1501 caatttggta ataaaaacat ccatactgcg tgctgtgaaa tagttgtctt caccccaaat
     1561 cttagttaac gcttcggatc gttcaaggac attattctgc cgcttgcaaa acattttgag
     1621 cagcatggct tccttaggag acagtttctc ttcatctgtt ggatccggtg tataggtcag
     1681 gatacgcaat ggaaagttaa acttgtattt gcctatgaca aaatccttaa cctcatcttc
     1741 aggatcggac ttgggtgcgc ttctcttgag caatgcctgg atgcggagta ataattcttc
     1801 agaagaaaat ggcttggtga tataatcgtc agcaccagca cgaaatccat gaaggatgtc
     1861 ttccttcatg tttttggcag tcagaaaaat aatcggcagg tccttatccc tttcccggat
     1921 ttcctttgcc agcgttaagc catcctttcg gggcatcata atatcaaaaa tagccaaatc
     1981 atatttcgaa ccccgataca actccattcc ggacactcca tccttgccca ggctgacttc
     2041 ataatcattc atttcgagat agcttttcag tacatcccca aaattttgat catcttccac
     2101 taatagtatt tttcgtccgt ttgtgcccat ttatcaatgt tgttttttct ttataacgta
     2161 tatcagtcct caatttgttg tttgtatgga aaataaagtg taaaagtact gcctttgccc
     2221 aactcagatt ttacatctac tgtgccttta tgtgcggtga tcatcgcctt aacatagctc
     2281 aatccaaggc cgaagccctt gacatcgtgc aggttgccag aagtagcccg gtaaaactta
     2341 tcaaaaatat gcttacgttg ctccttgttc attccgatgc ccttatcggt gacgataacc
     2401 tgtataccat cggcaacatt gcgtgtcctt accgtaattt ccggatactc cggtgagtat
     2461 ttgttggcat tgtccaatag attgtgaacc atgttggaga catgggtgat atctccctga
     2521 atgatatcgg ggtcggcttc gtcgaaggtt tcgatgtaac cacctttttg ttccacctgg
     2581 agattgatgt gtgtcacggc agacttaatc acatcgtgca aatgaatttc cgacaggttt
     2641 agcttcattt tcttccggtc gatgagtgcc atctgcaata ccttctctac ctggctcagc
     2701 atccggttgt tttcctgttt gatgatgtca aggaagcgtc gcaccttgtc agggctattg
     2761 atgatattgt cgttggaaat actatctgtg gcaaggctga tcgtggcgat gggcgtctta
     2821 aactcatgcg tcatattgtt gaggaagtcg tttttcatga tcgacaactt cttttgagtc
     2881 acaatctcat aaatggtaaa tataaaagta aacaatataa ttgccgtaaa caatgcggaa
     2941 cttagtacga tgccccacag gtttcggaaa gatgatctgt gtggaaaata tatcatcagt
     3001 tctccatgct caagactgcc gctgtcattg aaaatcgaaa ctttataggg tgtattgaac
     3061 agaccttgat ccatgttgtc gtgatagaca ttcttgttat tgttggtgac ggcataatat
     3121 ccgttgagga tgacgaaact cttcgctgtc tggctgtaca caccataatc atattcttcc
     3181 cggatatttt tatcggccag aaaagtcttg atataataat tgagctgctt gactttgatt
     3241 cgttcaccca aactggtctc attgaacatc cgacgcaggc tttcgtactc gaattcagtc
     3301 gccatagaac gactcccaga acgactgccg aaagtttcct gatacatgcg gtatctttca
     3361 tcattccgta actggtctgc caccttgttg agcacatcaa aaacatccgt ctggaacttt
     3421 tcttcagcag ttttcacggc actggaaatc cagtagtatt gtatccctgt cagcccaaac
     3481 aaggctattg acatcaaagt caccagtatc cagatcgtgc ttttcttcat aaattatgat
     3541 ggtatattgg acctgatctt tcaggatatt gctctatctc cactatataa cggagtcaaa
     3601 gataggaata tgtcaaactt acacatattt ccggaaatat acagccgaaa aatccacgat
     3661 tcttttgcta ataaatacct tactttgcgc ctatgaatta tctgacactc gagcatatca
     3721 agaaatccta cggtgacaaa gtcctgtttg acggtctgtc tatgtcgatc aatgaagggc
     3781 acaaaatcgc ccttgttgcc cgaaacggga caggcaaaac gaccctgctg caagtaatag
     3841 ccggtttgga aggcagtgaa ggcgaaaatg caaagattct cttcaagaaa ggcctccgta
     3901 tcgagtatct gacacaggat ccatcattta atcctgaaca taccgtgatc gatgcggtat
     3961 tcgacagtga caatcagaca ctcattgcag tgaaggaata tgaaagggcc ctgctcactc
     4021 ccggcaacga agcacggtta caggcagccg tcaatgacat ggaagacaaa aaagcctggg
     4081 atgtggaggc aagggtcaag gaaatcctca ccaaacttaa aataggcaat tttgaccaaa
     4141 aggtcaatca attgtccggt gggcaaaaga agagacttgc cttggcaaag gtattgattt
     4201 ccgaaccgga actgctcatc ctcgatgaac cgaccaacca tctcgatatg gagatgatcg
     4261 aatggctgga agaatatctg caacaccctg gccttactct cctactggtt acacacgacc
     4321 gatattttct ggaaaatgtc tgcaaccaga tccttgaact ggaaaatggt aatctgtata
     4381 aatattcggg aaactattcg gattatctgg aaaagaagca cgccaaggca gaaaatgaag
     4441 ctatcgtcct tgataaaaac aagaaactgt ttcttaaaga gcttgaatgg gtgcgtcgga
     4501 tgccacaagc cagaacgacc aaggcaaaag cgaggataga caagtttgat gagttgaaaa
     4561 atgaactttc gcaacagcgc gaagccgcca tactccagat caatatcaag tccaatcgcc
     4621 tcggttccaa gatcatcgaa gcgcacaatg taggcaaatc ctacaatgac aggcatcttt
     4681 tttcgcactt caattataaa ttcaaccgtt ttgaccgtgt aggcatcatt gggccaaacg
     4741 gatccggtaa atccacattc ctgaatatct tgacgggcaa tctgaaaccc gacgagggaa
     4801 aaatcgtaat aggagatacc gtcgtatttg gctactatac ccaatccgga atcaacctga
     4861 aggaagacaa gcgcgtgatc gacgtcatca cggatgtagc tgaatacata cccaccgaaa
     4921 aaggccataa tatcacagct gcatccctgc tggaaagatt catgtttccc cgggaacaac
     4981 aacaagtgta cgtatcccag ctgagtggtg gagaaaagcg cagactctac ctgcttacca
     5041 tccttatggc gaatccgaat tttctcatcc tcgacgaacc caccaatgat cttgatctgg
     5101 tcaccctcaa cgtattggag gacttcctca caacctacga aggctgtgta gtccttgtgt
     5161 cgcatgaccg ctatttcatg gacaaattgg ttgatcatct tttcgtcttc ggtaaggatg
     5221 agacgatcat cgattatccc ggcaactatt ctcaataccg agctt
//
LOCUS       DAOQQN010000228         5263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7083_length_5263_cov_4.291091, whole genome shotgun sequence.
ACCESSION   DAOQQN010000228 DAOQQN010000000
VERSION     DAOQQN010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7083_length_5263_cov_4.291091"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="PLG24_08945"
     CDS             <1..973
                     /locus_tag="PLG24_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HMZ25060.1"
                     /translation="VLTTLVNIQHRIGHGLELRREQFVADTAFRKDITESLDQQEVRS
                     GKHVEMLIENILNAYDNATGKAVKELDHGLSFPVMLKRSFSSIFSKKSTLKVWLQGVA
                     GQLEDDLNNELRKKLNDGAGDVAESIHQMAKMIQLKVQNSKTILKDDQDIFSDISERR
                     SYIMTDLQNTFNSFLQNSDNFKDQTLFPDQSGVSANIATGSGIAVIGLIIAGVTHGAV
                     FDVTGGLLTAVGLLFAGVTTMVKKNKIMSNFNQEIKKGKEKLESEISTQLIQYISHLR
                     HRIEDNFNRFDLMLAQEEKQIEAIDGAYKEVKGKLEQSGSELHRMLN"
     gene            complement(992..2098)
                     /locus_tag="PLG24_08950"
     CDS             complement(992..2098)
                     /locus_tag="PLG24_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25061.1"
                     /translation="MSSNKKNSKRGLPFRLGAQFISRGTSDLKAPEQFGHVLNKDQKK
                     KIRKLQWITYTISALIGATAVLIVILPYHLTAFFKPTHIEFWGISIDYDITYFVYAIA
                     IIFPEIWLLNVVNIRAVRTLCRIYRFPSVHSRDYNEQVDLLTEAGLEIPSSHMMSLGI
                     DPYVGMTRLSYYALFLFNKIKASLTNVAAKFIVRRLLGRYALRIVTDLVGVPIYAFWD
                     AWSSHLVIKETKLRISSYSAINDFMKDIRDEQLYAIRQKLPDLINFIAQQKRQYNFSL
                     YAFIQAILLRIPDLSLRTTKEIKAENLLTGNTQDDLLISHLMIFGMIIDGTLSLKERL
                     VLKNLSKEDWFPLTVSEVDDIVDGYIQGQGVRKF"
     gene            2316..2660
                     /gene="arsC"
                     /locus_tag="PLG24_08955"
     CDS             2316..2660
                     /gene="arsC"
                     /locus_tag="PLG24_08955"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537942.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HMZ25062.1"
                     /translation="MKTIIYHNSRCSKSREGLCLLEELGEDIEIRNYLKEPPTYEELE
                     NLLTMLNAVPLDIIRQKEALFKELYEGKHLTHQEWIKAMIEHPVLIERPIVVKGNRAV
                     VGRPPARIRELL"
     gene            2760..3152
                     /locus_tag="PLG24_08960"
     CDS             2760..3152
                     /locus_tag="PLG24_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25063.1"
                     /translation="MENKSNDATPLRPEGERILNAPMVEMDLEKFIGQIRSESTWANS
                     DHNSITIYKSDIMRMVLIGLHKGSVIKPHQTSSAISVQVLEGLIRFHTEEKSIDLSKG
                     KMVALQPKIQHSVEALEESFFLLSFANG"
     gene            complement(3180..4004)
                     /locus_tag="PLG24_08965"
     CDS             complement(3180..4004)
                     /locus_tag="PLG24_08965"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HMZ25064.1"
                     /translation="MNLLEKINETATYISNRINESPTVGIILGSGLGTLADAVEVSSE
                     LNYTDIPHFPVSTVIGHEGKLIFGKLGGKQCVMMKGRFHYYEGYDMLTVTFPIRVMKA
                     LGVQTLVVSNAAGGMNAAFQVGDIMLIRDHINQFPEHPLRGPSDKRLGPRFPDMSEPY
                     SLSLLKIAHQAASDLNLKVHEGVYVGVQGPTFETRAEYKWLHIIGGDAVGMSTVPEVI
                     VAVHAGMKVFAASIITDLGTPGHAGDISHEDVLKAANEAAPKLAAIVREVVARMED"
     gene            complement(4168..4341)
                     /locus_tag="PLG24_08970"
     CDS             complement(4168..4341)
                     /locus_tag="PLG24_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25065.1"
                     /translation="MRKNKKMPMYNKRKIYYSDMTKGLEAIESRVFCNNRFHYEEQFV
                     ANISNEFWVQPDI"
     gene            complement(5039..>5263)
                     /locus_tag="PLG24_08975"
     CDS             complement(5039..>5263)
                     /locus_tag="PLG24_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ25066.1"
                     /translation="INETIEHLPGNEGRITFLIAHRLSTIMHCDRIYVLESGRIVESG
                     KHTELLDQKGLYYAMWRQQVGLEPEPEEVF"
BASE COUNT         1488 a         1030 c         1121 g         1624 t
ORIGIN      
        1 tgtcctgacc accttagtaa atattcaaca taggatcggc catggcctgg aattgcgaag
       61 ggaacagttt gtagctgata ctgccttccg aaaagatatt acagaatcgc ttgaccaaca
      121 ggaggtcaga agtggtaagc atgtggagat gctcatcgag aatatcctca atgcttatga
      181 taatgcgacg ggaaaggcag tcaaggaact tgatcacggg ctttccttcc cggtaatgtt
      241 gaagcgatcc ttttcttcca ttttttcgaa gaaatccacc cttaaagtct ggttgcaggg
      301 tgttgccggg caacttgagg atgatctcaa taatgagttg cgcaaaaagc tgaatgatgg
      361 ggcaggagat gtggctgaga gcatccatca aatggctaaa atgattcagt tgaaggtgca
      421 gaacagtaaa accatcctga aagatgatca ggatattttt tcagatattt cagaaagacg
      481 aagctatatt atgacggatc ttcagaatac cttcaatagc tttcttcaaa attcagataa
      541 tttcaaggat cagaccttat tcccggatca aagcggcgtt tctgccaata tcgcaaccgg
      601 tagtggtatc gctgtgatag gattgattat cgcaggggtg actcatggcg ccgtatttga
      661 tgtcaccgga ggccttctga ctgctgtagg tctgttgttt gcgggcgtaa cgacgatggt
      721 caagaaaaat aagatcatgt ccaattttaa ccaggagatc aaaaagggaa aggaaaaact
      781 ggaatctgaa atcagcactc aattgataca gtatatttca catttgaggc accgtattga
      841 agacaacttt aaccgatttg atcttatgtt ggctcaggaa gaaaaacaga tcgaagctat
      901 cgatggagcc tataaagaag ttaaaggcaa gcttgaacaa tccggaagtg aattgcaccg
      961 tatgcttaat tgagttgcat ggccaacaag ctcaaaactt tctgacccct tgtccttgaa
     1021 tataaccatc gactatgtcg tccacctcgc tgactgtaag cgggaaccag tcttccttac
     1081 tcaggttttt caatacaaga cgttctttca aggatagagt cccatctatg atcataccga
     1141 atatcatcaa atggcttatc aacagatcat cctgggtatt tcctgtcagg agattttcgg
     1201 ctttgatttc tttcgttgtt ctgagcgata agtcaggtat gcgtaagaga atagcttgta
     1261 tgaatgcgta cagggaaaaa ttatattgtc tcttttgttg ggcaataaaa ttaatgaggt
     1321 cgggaagttt ttgcctgatg gcataaagtt gctcatccct tatgtctttc atgaaatcgt
     1381 tgattgccga atatgatgat attcgcagtt tcgtctcctt gatgacaagg tggctggacc
     1441 atgcatccca gaaagcatat atcggcaccc caacaaggtc tgtaacaatc ctgagggcat
     1501 aacgtcccaa taaccttctg actatgaatt tggcagctac attggtcagg cttgctttga
     1561 tcttgttgaa tagaaaaagg gcgtaatagg ataatctcgt cataccgaca taagggtcaa
     1621 tccccagtga catcatgtgg ctggacggaa tttcaaggcc tgcttccgtg agaaggtcaa
     1681 cttgttcgtt gtaatctctg ctgtggactg acgggaatcg atagattctg cacagtgtcc
     1741 ttaccgcacg gatattcacc acatttaata accagatctc cggaaaaata attgcaatag
     1801 catacacgaa gtatgtgatg tcataatcaa tggatattcc ccaaaattct atgtgtgttg
     1861 gtttgaaaaa ggcggtcagg tgatagggca gtatgacaat caaaaccgct gtggcaccaa
     1921 ttagtgctga tatagtgtaa gtaatccact gaagtttcct tattttcttt ttttgatctt
     1981 tgttgagcac atgcccgaat tgttcaggcg ctttcaaatc cgatgtgccc cggctgatga
     2041 attgtgcgcc aagtctgaat ggcagtcctc gtttgctgtt ttttttatta cttgacatgc
     2101 taaatgaagt caaaaataaa aattgtcccg aattgcacgg ttcaaattat ttgcgatttt
     2161 atggttaggg cgcacctgat gaaaataaga taaattggtg ttttatattt gtaaaaaatt
     2221 tattttgtgc taaaattctt tatctttata attcaatggg ttgtcgacac tcaggactgg
     2281 aatcattttt ttgaaatctg aatttgtgta tttgtatgaa aacaatcatt tatcataatt
     2341 ccagatgcag taagagcagg gaaggtcttt gtctattgga agaattgggt gaggatattg
     2401 agatcaggaa ttatctcaaa gaacctccaa cttatgagga gttggagaat cttctcacaa
     2461 tgctcaatgc cgtccctttg gatatcatac ggcaaaaaga ggcactcttt aaggaattgt
     2521 atgaaggtaa acacctgaca catcaggaat ggatcaaggc aatgatcgag catcctgtcc
     2581 tgatagaacg tccaattgtg gtgaaaggca atagagctgt tgtaggcaga ccacccgccc
     2641 ggatacgtga actattgtga aatgtccggg tgaaagagag tagaaaagtc tttacggaat
     2701 tgcttgattg aaaacttagt tacctttaaa tttacctgat aattcaaaca taatgtccta
     2761 tggagaataa atcgaatgat gcaacacctc tgagacctga aggagaacgt atattgaatg
     2821 ctccaatggt tgagatggat ttggagaagt ttatcgggca gattcgcagt gaatcaacat
     2881 gggcaaacag tgaccacaat tctattacta tctataaaag tgatataatg aggatggtcc
     2941 tcattggatt gcataagggc tctgtgatca aaccgcatca aacatcttca gctatcagcg
     3001 ttcaggtcct ggaaggattg atccggttcc atacggagga gaagtccatt gatttgtcca
     3061 aagggaagat ggtggcgttg cagcctaaga ttcaacatag tgtcgaggct ttggaagaaa
     3121 gttttttcct gctttcgttt gctaatggtt aaatagccaa taaacatgag tctgcccggt
     3181 taatcttcca tccttgcaac tacttctctg acgatagctg ccagttttgg cgctgcttca
     3241 ttagctgctt tcaatacatc ttcgtgggaa atatcaccgg catgtcccgg tgtgcctaag
     3301 tccgtgatga tgctggcagc aaaaaccttc atgccggcat gtactgctac gatgacttcc
     3361 gggacagtgc tcatacccac cgcatcgccg ccgatgatgt gcagccattt atactcggca
     3421 cgggtttcaa aggtcggtcc ctgcacgcct acatagacac cctcatgcac cttgagatta
     3481 aggtcggaag ctgcctggtg agctatcttt agcaatgaca gggaatacgg ttcactcatg
     3541 tcagggaatc tcggtcccaa ccttttgtct gatgggccac gtaagggatg ttccggaaat
     3601 tggttgatat gatcccggat cagcataatg tcgccgacct ggaatgcagc attcatacct
     3661 cctgctgcgt tggatacaac aagtgtctgt acacccaggg ctttcattac acgaatggga
     3721 aaggttacag ttaacatatc ataaccttca taataatgga agcggccctt catcattaca
     3781 cattgcttcc cgccaagttt gccaaatatc aatttccctt catgaccgat gacggtggaa
     3841 acaggaaagt ggggaatgtc ggtataattc agttcgctac ttacttcgac tgcatctgca
     3901 agcgtgccta agccgcttcc gagaataatg cctaccgtag gcgattcgtt aatccgattg
     3961 ctgatgtagg tggcggtttc attgattttt tccaatagat tcatgtcgta tgtgcttaat
     4021 ggcgccaata aatgtagggc aatataatga atatgactga taaatttttt cgtgatttga
     4081 agtcaagatt ggctcttatt tcaatgcaag attataaaat gcagtaaata aataaaatat
     4141 gcatgataaa ataaccgtat attttaatca tatatcaggt tgcacccaaa actcattaga
     4201 gatgtttgcg acaaattgtt cctcgtagtg gaacctgttg ttgcaaaaga ctctgctttc
     4261 gattgcttcc aaacctttgg tcatatcaga ataataaatc ttacgcttgt tgtacatggg
     4321 catttttttg ttctttctca ttcgaaatcc gggataaata cccgtctttt cattttatcc
     4381 ttgtctgtgg aattcagttt gattaaagca ttcttcttcc ctgtacgatc attgtacagc
     4441 tctttttgta gatcatcaga tttttcaaat acatttggtg tcttttagct taaggttgat
     4501 tgaagcttca gatatctata aatcgctttt ttgcatccgc cgcattccct caattcctga
     4561 gtccttgatt atttcattca ttttaattct cgctgaacag cctgttgatg cataaattgc
     4621 ttgttatata tgctcctgca ttgacttatg atatattaat catctaatta atgcattttt
     4681 tttaaatcaa cttttgacag actattcttt ccggttcatt ttctcacttc tttgcaattt
     4741 gaaggcttga ggagcacttt atatattctt gctattcaat aatttatttg gtaaaacata
     4801 aagtgttgct acaacacgcg atggatattt gaattttctt ttgagtcagt tttttcttaa
     4861 atgaggtgca ctaaattacc ctgcttctta taaaatgtgg gttgcaccaa aaatttttgg
     4921 actattatat taattcatct gggtaaaatt tctcgaaaac ttcagaataa agctagtatt
     4981 tgcgggattc cttttgcttt tcagtaaatt tacttggttg ttttttcaac ctttttactt
     5041 aaaaaacctc ttcaggttca ggttccaacc cgacctgctg tcgccacatg gcgtaataca
     5101 agcctttttg atccaaaagt tcagtatgct taccggactc gacgattcgc ccgctttcaa
     5161 gtacatagat gcggtcacaa tgcataatgg tactcaggcg atgagcaatg agaaaggtaa
     5221 ttctgccttc attacccggc aggtgctcaa tggtttcgtt aat
//
LOCUS       DAOQQN010000229         5239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7155_length_5239_cov_4.476080, whole genome shotgun sequence.
ACCESSION   DAOQQN010000229 DAOQQN010000000
VERSION     DAOQQN010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7155_length_5239_cov_4.476080"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2032)
                     /locus_tag="PLG24_08980"
     CDS             complement(<1..2032)
                     /locus_tag="PLG24_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HMZ25067.1"
                     /translation="MKQLLVSVGLILSIGLHAQVTIDPPFPNINSSITVYFDATKGNQ
                     GLKDCNCTVYAHAGLITDKSSSGSDWKYVIGNWGTDDSRVKMNKVSDNLYSLTYNIKS
                     FYGIPESTIIRKLAFVFRNLNGSKEGKTESNGDIYYDFPDPNAGLQYLIQSPSLESRL
                     VSKNSVLNLSVLTSIPADLRFTINGQDVVTTHDVSMSYQYIADQVGTFRFEIGIHAGN
                     EGLDTSFVWTVGGDPEISEVPDGLLLGCNKVEEGKAIVLLEAPRRNIAFLVGNFSNWA
                     PLPEYQMKQSPDGQFLWGEIDTVGLQGDLDYQIWFGDGLKIPDPLSVEILDDVNDKYI
                     PAAVYPDMPSYPEGKTTGLVSHLKLNADYDWQDEAFVKPAVGDLVIYELLVRDFLASH
                     DFDDLIDTLLYLKRLGVNAIELMPVSEFEGNISWGYNVSLHNAIDKYYGGASKLKKFV
                     DAAHGLGFAVIADVVFNHAFGQNPMVRMYWNAAQNKPAIDNPWFNPDAKHPFNVGYDF
                     NHQSAATRRYVSQILREWIREYHFDGYRFDLSKGFTQVNNPNDVGAWGQYDPSRIEIL
                     KYYSDQIREVDPQAYVILEHFADNQEEKELSDYGMMLWGNINYEYAESVKGNSASLNR
                     SYFRQRGWRKPHLVSYMESHDEERVMYKALKEGRQSAGYNVRDLKTALGRVGM"
     gene            2560..3249
                     /locus_tag="PLG24_08985"
     CDS             2560..3249
                     /locus_tag="PLG24_08985"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HMZ25068.1"
                     /translation="MQFFQLFLLQIPDTTAIQSIALPTQKLTFFELLLKGGVIMIPLV
                     ILSIIALGLIIERWLSINNLTKVEANFFNNIKSMLSNGNVKGAQDFAESNNTSIGKVI
                     GEGIRYIGYPYSEIETYMSDTANIELGKMENRLNYLGIIAGVAPMLGFVGTIAGIIKI
                     FYNISITDNISIGVISGGLYEKMIASGTGLIVGIFAFIGNQMLQAKIDRYALKMQDMV
                     KSFRSVLLQKG"
     gene            3311..3706
                     /locus_tag="PLG24_08990"
     CDS             3311..3706
                     /locus_tag="PLG24_08990"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMZ25069.1"
                     /translation="MKINRKRHKPHVFVDSLSDIMFFLLLFFLIVSTVANPNVVKILL
                     PKSGNTEVLSKKQITLTITADKRYFIDKQEYTIDQLEEAISQVTANVTEPTIMLRLDK
                     SLTVQDLIDVMSIGAKLKIKMVLATDKKS"
     gene            3708..4757
                     /locus_tag="PLG24_08995"
     CDS             3708..4757
                     /locus_tag="PLG24_08995"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMZ25070.1"
                     /translation="MPAKFKVGLCAYGMSGKLFQAPFFAVHPGFSLGYIVRRSNPASI
                     TEYPDTQILRSTEALIAKPDIDLIVVNTPVQTHYEIAKQALATGKHVLVEKPFTVTYR
                     QALELIDIAREHNAKLSVFQNRRFDRDYLRVKTILESGNLGQIHEATIRFDRYRTGSS
                     GKLHKEGNFPGSGALYDLGSHLIDQALQLFGPPENIYADLMQLRPDVESDDYFELLLY
                     YQQLRVRLISNVMVTLPEPGYVLNGELGSLSQLRSDMQEKELGVGICPSSENWQKPLS
                     EPDSILTYHEQNNIKRKESFSDPGNYFWFFDALHQFLNGRGPNPVSPEQAAEVIRIIE
                     IAKESSTAGKKISCK"
     gene            complement(4795..>5239)
                     /locus_tag="PLG24_09000"
     CDS             complement(4795..>5239)
                     /locus_tag="PLG24_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HMZ25071.1"
                     /translation="DALEPFRTAGPVQDPEVLNYLATRGSKSFDIPISATLTTKEGVE
                     TVICETNYKFLYWNPSQQLAHHTVNGCNVNIGDLMASGTISGSDPSSYGSMLELAWKG
                     TKPIQLADGSERTFLIDGDSITLRAKAVSGEVVVGFGEAKGTVLP"
BASE COUNT         1628 a         1157 c         1000 g         1454 t
ORIGIN      
        1 ccatccctac cctcccgagt gcagttttca gatcccgaac attatatccg gcggattgac
       61 gcccttcctt caatgccttg tacattaccc gttcttcatc atggctctcc atataggaca
      121 ccaaatgcgg cttacgccag cctcgttgcc ggaaatagct acgattcaaa ctggctgaat
      181 tgcctttgac actttcagca tattcataat tgatatttcc ccaaagcatc atgccatagt
      241 cagacaactc cttttcctcc tgattatctg caaaatgctc aagaattaca taagcctgag
      301 gatcaacttc tcttatctgg tctgaataat atttcaaaat ttcaatgcgg gatggatcat
      361 actggcccca tgctccgaca tcattcggat tgtttacctg tgtaaagcct tttgataagt
      421 caaagcgata cccatcaaaa tgatattcac ggatccactc cctgagtatc tgactgacat
      481 acctgcgtgt tgcagcgctt tgatgattaa agtcatagcc gacattgaat ggatgtttgg
      541 catcaggatt aaaccaggga ttatcaattg ccggtttgtt ctgtgcagca ttccaataca
      601 ttcggaccat aggattttgc ccgaaagcat gattgaacac aacatccgca ataacagcaa
      661 aacccagacc atgagcggca tctacaaact tcttcaactt cgatgcacca ccataatatt
      721 tgtctatggc attatgtaga gaaacattgt atccccaact gatattacct tcaaactctg
      781 agaccggcat aagctctatc gcattgactc ccaagcgctt cagatatagc agggtatcta
      841 ttaaatcgtc aaaatcatgg gatgccagga aatcccgtac cagtaattcg taaatgacca
      901 aatcaccaac agcaggtttg acaaaagcct catcctgcca gtcataatca gcattcaatt
      961 tcagatgaga caccaatccc gtagtttttc cctccggata cgacggcatg tcgggatata
     1021 cggcagccgg gatatattta tcattgacat catccaatat ctccacactg agtggatcag
     1081 gaatcttcaa accatcccca aaccaaatct gatagtctaa atcaccttga agtcccacag
     1141 tatcaatctc tccccaaaga aattgtccat ccggtgactg cttcatttga tattccggta
     1201 atggcgccca attactaaaa ttacccacca gaaaggcgat attacgccgt ggcgcttcca
     1261 aaaggacaat agccttacct tcctcaacct tattgcaccc caatagaagt ccatctggca
     1321 cttcactgat ttcaggatct cctccaactg tccatacaaa tgaagtatcc agaccttcat
     1381 tacctgcatg gattccaatc tcaaaacgga atgttcctac ctggtcagca atatactggt
     1441 aagacatgga tacatcatga gttgttacca catcctgccc attgatagta aaacgcaaat
     1501 cggccggtat agaagttaat acgctcaggt tgagaacgga attcttagat accagtcgac
     1561 tttcaagact tgggctctgt attaaatact gtaatccggc atttggatcc ggaaaatcat
     1621 aatagatgtc tccatttgat tcagtcttac cttcttttga tccattgagg ttacggaaga
     1681 caaaagccag tttcctgata attgtactct caggtatccc atagaatgac ttgatattgt
     1741 atgtcagaga atacaggttg tcacttacct tgttcatttt aaccctggaa tcgtctgtcc
     1801 cccaattgcc gatgacatat ttccagtcgg agccgctgct gcttttatcg gttatcagcc
     1861 cggcatgagc ataaacagta caattgcaat ccttaagccc ctgattaccc ttagtagcgt
     1921 caaaataaac agttatggaa gagttaatat tgggaaatgg cggatcaata gtcacctgtg
     1981 catgtaaacc aattgataag atcaggccaa ctgagacaag cagttgtttc atgtttttaa
     2041 aaaattattt atgcaagata aggagaatag cgcattatga aaaatgaata gtgaagaaaa
     2101 cctgaagtgt aaagaaagtt atgtataact caagagccaa acaatagaat tatacatccg
     2161 gaaagtaaac ctccggagta gaaaaaagaa ggcaaatcaa gtcccggaga agcataccta
     2221 accaacaaga gtatgatccc tttccaaaaa ttgacttcaa tccatacagg aagccggctt
     2281 aacagattgt aaataaacca acaatatata gatcattcca atatatagca acaaaacaaa
     2341 ataaaatcaa tctgccttcg ttaaatcatg aaagacaatt aaaatctgcg caataattta
     2401 cttcagaaaa tgcaaagctc ctaaaaaggg agttttcgtc ctcaaaacac aataattatc
     2461 tgtttttgaa aaataaagta aaaaaacata taagttattt tttaaatata ttacctttgc
     2521 tgattaaagt ttttaaatca agttctaaat ccggatttta tgcaattttt ccaattgttt
     2581 cttctacaga ttcctgatac cactgctatt caaagcatcg ctcttcctac tcaaaaattg
     2641 acctttttcg aattgctgtt gaaaggtgga gtgatcatga tacctttggt gatactgtct
     2701 attatagccc tggggctgat tatagaaaga tggttgtcta tcaacaatct aaccaaggtg
     2761 gaggccaatt tcttcaacaa cattaaatct atgctatcca atggtaatgt caaaggcgcg
     2821 caggattttg ccgagtccaa caacacctcc attggcaaag tcatcggaga aggtataaga
     2881 tatattggct atccctattc tgaaatcgaa acctatatga gtgacactgc aaatatcgaa
     2941 ctgggaaaga tggaaaacag gctcaactat cttggaatca ttgcaggggt tgctcctatg
     3001 cttgggtttg ttggtactat cgccggtatt attaaaatat tttataatat atcaattacc
     3061 gataacatct ccataggagt catttccgga ggactttacg aaaaaatgat cgcatcaggt
     3121 accggcttga tagtaggtat atttgccttt ataggaaacc agatgcttca agcaaaaatc
     3181 gacaggtatg cgttaaaaat gcaggacatg gtcaaatcct tccgctccgt actgctacaa
     3241 aaaggctaac gtatatttct ttctgaaata tgattgttca ttctatgatt aaccccgaaa
     3301 gaaatatcag atgaaaataa atcgaaaaag gcacaaacca catgtattcg ttgattcgct
     3361 cagcgatatc atgttttttt tgttgctctt cttcctgatt gtctcaacag tggccaaccc
     3421 caatgtcgtg aagatccttc tcccgaaatc gggaaatacg gaagtattga gtaaaaaaca
     3481 gattactttg accattacgg ccgataaaag atactttatc gacaaacaag aatataccat
     3541 agaccaatta gaggaggcga tttcgcaagt cacagccaat gttacagagc caaccatcat
     3601 gttgaggctt gataaatccc tgactgtcca ggatctgatt gacgtaatgt ctatcggagc
     3661 caagctgaag atcaagatgg tattggctac agataaaaaa agttgatttg ccggctaagt
     3721 ttaaagtagg tctgtgtgct tacggtatga gtggtaagct gtttcaggca cctttctttg
     3781 ccgttcatcc gggattttct ttgggttaca tcgtaagaag atctaatccc gcatcaatca
     3841 cggaatatcc cgatacgcag atactccggt ccactgaagc tttgattgcc aagccggaca
     3901 ttgacttgat tgtagtcaac actcctgttc agactcatta tgaaattgca aaacaagcac
     3961 ttgcaaccgg aaagcatgtt ttggttgaga aaccctttac ggttacctat cgtcaggcgc
     4021 tggaattgat tgatattgcc agagaacata acgcaaagct ttcggttttc caaaatcgcc
     4081 gattcgaccg ggactacctg agggtgaaga ctattcttga aagcggaaat cttggtcaga
     4141 ttcacgaagc aacgataaga tttgaccgtt accggacagg atcaagtgga aaattacaca
     4201 aagaaggcaa cttccccgga tccggcgctt tgtatgatct ggggagccat ttgatcgatc
     4261 aggctttaca attgtttgga cctccagaaa atatttatgc cgatctgatg caattgagac
     4321 cggatgtcga atccgatgat tattttgaat tattactgta ttaccaacaa ttgcgggtcc
     4381 ggcttatcag caatgtgatg gtaacgcttc cggaacccgg atatgtatta aatggggagc
     4441 ttggttccct cagccaactt cggtcagata tgcaggaaaa agaattggga gtcggaatat
     4501 gtccttcatc cgaaaactgg caaaagcctt tgtcagaacc ggatagcatc ctgacctatc
     4561 acgaacagaa taatataaaa cggaaagaat cattttccga ccctggtaat tacttttggt
     4621 tttttgatgc attacaccaa ttcctcaatg gaaggggtcc taatccggtc tcaccagaac
     4681 aagctgccga agtaatcaga atcatcgaga tagcaaaaga atcttcaaca gccggaaaaa
     4741 agataagctg taaataaatt tcaacaattg cctgaaagaa ttgggaggcc agttttatgg
     4801 caataccgtt cctttcgctt ctccgaaacc aacaacaact tcaccgctga ctgcttttgc
     4861 ccttaaagta atggaatcac catcgatcag gaaggttctt tcactaccgt ctgccaactg
     4921 gattggtttg gtacccttcc aggccagttc gagcatagaa ccataagacg agggatcaga
     4981 gcctgaaatc gtaccggatg ccatcaaatc accgatatta acattgcagc cattgacggt
     5041 atgatgcgcc aattgctgac tgggattcca gtaaagaaat ttataatttg tctcgcagat
     5101 cacagtttcg accccctcct ttgtggtcag agtcgcactt attggaatgt caaaactttt
     5161 tgatccacgg gtagcaagat agtttaatac ttccggatct tggacaggtc ccgcagtcct
     5221 gaatggttca agagcatcc
//
LOCUS       DAOQQN010000230         5232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7172_length_5232_cov_5.357929, whole genome shotgun sequence.
ACCESSION   DAOQQN010000230 DAOQQN010000000
VERSION     DAOQQN010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7172_length_5232_cov_5.357929"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(822..2030)
                     /locus_tag="PLG24_09005"
     CDS             complement(822..2030)
                     /locus_tag="PLG24_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25072.1"
                     /translation="MRKLLLLFAAIVFFTTYVKGQLVYHDAPPYQDEKTLRHVSEKAL
                     EVIDSIIHYGLNTRRADIFEIKNEQYLLVDGTFDVFHWESGRWHNLYEGIFYGYNFGS
                     KKFIIDDKIYSFGGYGYWNKHGQIIQFLKDRGSWELVDMDQIPPYGIACSKNRDLTVF
                     SSDSIFYVNIDRKTMVARPLIRKMVEKSDLPNRYTIELKNYTLIYGRNPFYLIEKKND
                     SIFESNLSPFKGILNALKNGMIYVKGDSMFCYDRSFKLEGAYDAQSERDFFKPIKVVN
                     NSILGGFSKWLIVLAAGLLIAFIIYFATRKKHKPFLEEGILSEAELDLEHPLIIKLQN
                     FNGVGLSQDQLDEIFEINHILSPESKRFRRSQQIKEINLIYRTSQQMDMITRVKDPTD
                     KRKFIYQIQS"
     gene            2581..3723
                     /locus_tag="PLG24_09010"
     CDS             2581..3723
                     /locus_tag="PLG24_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ25073.1"
                     /translation="MTTTQESMEMIRQSAKDFAETHIRPYMMEWDESQYFPVETMREL
                     GRHGFLGVLVPEEYGGSGLGYQEYITVIEEITKVCSSIGLSLAAHNSLCTGHILAFGN
                     EEQKKKYLPKLATGEWIGAWGLTEANTGSDAGRMQCVATKDGDYYIINGTKNWITHGK
                     SGEVAVVIARTGELLDSHGMTAFVVERGTPGFSGGKKENKLGMRASETAEMIFDNCRV
                     HKSQVLGQEGDGFIQAMKVLDGGRISIAALSLGIAKGSYEASVKYAKERHQFGQPIAN
                     FQGISFKLADMATKIEAAELVTRKAADLKNKGKKMTKEGAMAKYYASEVCVWAATEAV
                     QIHGGYGYTKDFPVEKFYRDSKLCTIGEGTSEIQKVVISREVIKGS"
     gene            4150..4458
                     /locus_tag="PLG24_09015"
     CDS             4150..4458
                     /locus_tag="PLG24_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HMZ25074.1"
                     /translation="MKKQFVKSKPVVKVTFNPTAEVVNGAKEVLVLGDFNNWNSELGF
                     ELKKQKDGSFKGIMELEKGHEYQFRYLIDGSKWENDHDADTVVAGPYGASNSVVSTIE
                     "
     gene            complement(5143..5217)
                     /locus_tag="PLG24_09020"
     tRNA            complement(5143..5217)
                     /locus_tag="PLG24_09020"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5180..5182),aa:Pro,seq:tgg)
BASE COUNT         1592 a          855 c         1037 g         1748 t
ORIGIN      
        1 agtttttaaa agaagtataa aaattcattg tgtttttaca ggctgaattg aatcgtaaga
       61 attaagcttt tgtccatact gcaccaattt ccatttccaa aggttttgtt ataatgtgcc
      121 ttcaaatggt gatcaactta aatcttaagt acagcaaaat ttgatgtaga gcatattatt
      181 gatacattga ctgtaataag tcatgacttc attttacttg ccattgtttt cagtgggata
      241 aatagctgat ttagccaaag gccgacatgc ccgttcggaa gtgccatttc cttcaatcag
      301 ccattatccg gaaatatttc acaagattcc gattgaatga tttgtgcaaa tcggtaatat
      361 tatggtttaa atacaccgat ttctacgata agattcatga tttttgataa tatttcttct
      421 aggacattag tcgcctgaat gataagcttt catcgtataa aacccggatt ctggctggta
      481 atcagaagtg tcaaatttcg gtttaaaccg gtttatcata ggtttaagag gatgtagtta
      541 ataaatttgc ctcaatactt aacaaaaatc aatcatttta atgaaaagta tgatttctgt
      601 ttttattcct ccaatcttat gaaatctttc aaaaataaat acaaaccaat tggggaattt
      661 tgtacatgat ctatcttatc ccttatggaa aagtctaaat taatcccatt aatttaaaaa
      721 cactttagtt aaagaccgga tgataccatt ccggtttttt tatttaaacc aaagtgaaga
      781 ataaatcatg ttagctcagg gtaattgaat ccgttaaatt cttagctctg tatctggtag
      841 ataaattttc ttttatctgt aggatcttta acccttgtaa tcatatccat ttgttgcgat
      901 gtccggtaga taagatttat ttctttgatc tgttgagaac gtctgaaacg tttggattca
      961 ggtgaaagga tatggttaat ttcgaagatt tcatccagct gatcctgact aagacctacg
     1021 ccattaaagt tttgtaattt gatgataagt gggtgctcca ggtcaagttc tgcttcagat
     1081 aggatgcctt cttccagaaa tggcttatgc ttttttctcg ttgcaaaata aattataaat
     1141 gcgattaaga gaccagcagc caacacaatc agccatttgc tgaaacctcc caaaatgctg
     1201 ttattgacaa ctttgattgg tttgaaaaaa tccctttctg attgggcatc ataggcacct
     1261 tcaagcttaa aggatctgtc atagcaaaac atgctgtcac ctttgacata aatcattcca
     1321 ttctttaaag cgtttaagat tcccttaaaa gggctcaaat tggattcaaa tatggaatca
     1381 ttcttttttt caatgagata aaatgggttt cttccataaa taagtgtgta attcttaagt
     1441 tcgatcgtat acctgttcgg cagatcactt ttttcaacca tctttctgat aagtggacgt
     1501 gccaccatgg tcttccggtc tatattgaca taaaaaatag aatctgaaga aaaaacggtc
     1561 aaatcccggt tttttgaaca agctatccca tatggaggaa tctgatccat atctactaat
     1621 tcccagctgc ctctgtcttt cagaaactga attatctgac catgtttgtt ccaatagcca
     1681 tatcctccga aagagtatat tttgtcatca attataaact ttttgcttcc aaaattatac
     1741 ccgtagaaga ttccttcata taagttgtgc catctgccag attcccagtg aaatacatca
     1801 aatgtgccat cgactaacaa gtattgttca ttctttattt cgaaaatatc tgcccgacgg
     1861 gtatttaagc cataatggat gattgagtct attacttcaa gcgctttttc actgacatgg
     1921 cgcaacgttt tttcatcttg atagggaggt gcgtcatgat aaaccaattg gcctttgaca
     1981 taggtagtga aaaaaacaat tgcagcaaat agaagcagta attttctcat tcgaggcagg
     2041 atttggaaaa atagatatct tttatttgaa tattaaccgg tatttctaaa ttaaagtcaa
     2101 aattaatcag aaaacataat atttcaaaaa aagatgatat ttcaacggat tgaaactttc
     2161 ttttacttac cggattattt tatttctttg gttaaggtaa cctaaatatc taagtcatcg
     2221 tgaggggtat ttctgccgtc aatattgcag gatagatgta taataaatat tctttttaac
     2281 aatgagaata ggcgtatcat agataatttt cgggataaat aattatatgt ttacccaata
     2341 atttaaaacg gggcactcta agttgcacat aagttgatag ctacttgatc tacaataata
     2401 tccgataatt aaaatttgat ataataagat taggttatgt cagaatagta tccacaaagc
     2461 ttgtagtttg tttttccttc cattttttca tttgtttatt gtgtgatgca ctcaccttgt
     2521 ctcacagcat ttgatatttg tctaaaaagt tatagttttg cgaacttaaa tttaggaata
     2581 atgactacga ctcaggaaag catggaaatg ataaggcaaa gtgcaaaaga ctttgcagaa
     2641 actcacataa ggccttatat gatggagtgg gatgaatctc agtacttccc ggtcgaaact
     2701 atgcgtgaat tgggccgtca cggttttttg ggcgttttgg tacctgaaga atatggcggg
     2761 tccggactgg gttatcagga gtatattaca gtaattgaag aaataactaa ggtttgttct
     2821 agtatcgggt tgtcattggc agcacataac tctttgtgta caggtcatat cctggctttt
     2881 ggcaatgaag agcaaaagaa aaaatatctg cctaagttag ctaccggaga gtggattggt
     2941 gcatgggggc ttacagaagc caatactggt agtgatgctg gaaggatgca atgcgtggcc
     3001 acaaaggatg gtgattatta tattatcaat ggaacgaaaa actggataac gcatggaaag
     3061 tccggtgagg ttgcagtcgt tatagcccgt accggtgaat tacttgattc gcatgggatg
     3121 acagccttcg ttgttgaacg cggcactccg gggttttcag gaggcaagaa ggaaaacaaa
     3181 ctgggaatgc gggcatcgga aacggcagaa atgatcttcg acaattgcag ggttcacaaa
     3241 tctcaggtgt tgggacagga aggagatggc tttattcagg ccatgaaggt gttggatggc
     3301 ggtcggatat ccattgcagc gctttcactc ggcattgcta aaggtagtta tgaggcatct
     3361 gtgaagtatg ccaaggaaag gcatcagttt ggacagccaa ttgccaattt tcagggaatt
     3421 tcattcaaat tggctgacat ggcaaccaag attgaagcag ctgaattagt taccagaaaa
     3481 gcagctgatt tgaagaacaa aggcaagaag atgactaagg aaggcgccat ggctaaatat
     3541 tatgcttctg aagtctgtgt ctgggcagct actgaagccg ttcagattca tggagggtat
     3601 ggatacacca aggattttcc ggtcgaaaaa ttctatcgtg acagtaaact ttgtacaatt
     3661 ggggagggaa catccgaaat tcaaaaagtc gttatatcca gagaggttat taaaggctcc
     3721 tgataggtct tgattcatta agttttattc ctaaaggttc ttttattata ttttttctta
     3781 aggagatatc tgtggctctt ttcctgattt atttttgatg gtgaccctga ttcctcacgt
     3841 tttttatact ccaaactgtt cgatgtgctt tcatccgata gagctatcta tttaattaac
     3901 ttattgttaa caaaatatat gttaatgacg gagtcgtttt tacaatcttt ttaaaaataa
     3961 tatatggcat aaatgctatt tgctcaaata attggtgttt gacaaattga taaaacttat
     4021 ttataaatag ctacttctca attccttaaa atttatctgt tttattaaat agtgattgtc
     4081 aatgttttgt aggttttgtt ttgtttgtat ctttgccgaa tattattctc aatacttaat
     4141 tttttattaa tgaaaaagca atttgtaaaa tctaaacctg tagtaaaggt tactttcaac
     4201 ccgacagctg aagttgtcaa tggtgcaaaa gaagttttgg ttcttggtga tttcaacaac
     4261 tggaattctg aattaggctt tgaactgaag aagcaaaagg atggctcctt caaaggtata
     4321 atggaacttg aaaagggaca tgagtatcag ttcagatatc tgattgatgg aagcaagtgg
     4381 gaaaacgatc atgatgctga cactgttgtt gccggtcctt atggcgcttc taactctgta
     4441 gtttctacca ttgagtaatt gaatctttga attttactga caatataaag ctgccttagg
     4501 gcagcttttt tattttgtag aaggccgggg ccatttttca cataatacca tttttaaagg
     4561 tacttttggt tgatcgttct caatttttat tttttagatt aaatttcctg agcatctatt
     4621 tatctctctg catgagaagg tgtagattaa tttgttgtta ggttgggaat tgtattcgga
     4681 ataaaagttt agtttaaatg ttgaggggtc gttaagctgt gttccattat tttattaccc
     4741 ttaagttatt ggcccggatt atttatatat tgtaggtgtt ctgcctgtta agagccggat
     4801 tgttgaaagt tgtaatagtt tgatcaaagt ggggtagggt aaggagagca agcaaagaaa
     4861 tttgatacct gatatgaatg attcttttaa tagtccataa tttgaatgta ggctgatgca
     4921 ctgattgctt tctggctaaa gtagcctgcc ttgaaggggt acatttggta aatatgatta
     4981 acagaaagga tgccacagat gctaattgct ctattcaccc tcatcataga tcccgaaatg
     5041 tatgttctgc aacttgattg gatgactaat aatagcttta aattattact tctgaatacc
     5101 agaagacaca aaaaaaggct gccctttgga caacctctct tgtcggggta gcaggattcg
     5161 aacctgcgac cacctgctcc caaagcaggt acgctaaccg gactgcgcta caccccgaag
     5221 ttttgttaag tg
//
LOCUS       DAOQQN010000231         5199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7244_length_5199_cov_4.721812, whole genome shotgun sequence.
ACCESSION   DAOQQN010000231 DAOQQN010000000
VERSION     DAOQQN010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7244_length_5199_cov_4.721812"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLG24_09025"
     CDS             complement(<1..399)
                     /locus_tag="PLG24_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HMZ25075.1"
                     /translation="MEKEHILGLDIGGTGIKGAVIEVKTGKLLTERVKIMTPEPSTPE
                     AVGKVAAEIKHKFGFEGIIGCGFPAIIKNGVAMSASNIDQSWLNTDVQEVLGRHTGSR
                     VFVANDADVAGMAEQTFGFAANQHGTVIVLT"
     gene            complement(530..1609)
                     /locus_tag="PLG24_09030"
     CDS             complement(530..1609)
                     /locus_tag="PLG24_09030"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HMZ25076.1"
                     /translation="MASTKAFPRFPQVRPSLHSELRRRVSLYFEENKIRQTGNANLYW
                     KAAILLTLFALTVGHLFIFQPHWAIAYSECILLGLLVATIGFNIMHDGSHGSFSTHQS
                     SNRLASYTMSMLGASHFFWNIKHNVIHHTYTNVDDLDDDIEVGKLMRLAPSQTRLPFH
                     KYQHIYFPALYMVMYLYWVFAADYKKYFKMKIGEFPITNMKMSDHFRFWLVKIWHALF
                     FCVLPIYIFGFWPWLLGFIIMSLTAGFCLSIVFQLAHTVEDTSFPLANEDTNKFDDEF
                     ATHQLKTTANFGTKSKTISWLVGGLNFQVEHHLFPKISHIHYPGISKIVRKVCDEYQI
                     KYIEYRTMWSAIMAHIRFLKAMGRA"
     gene            complement(1633..2514)
                     /locus_tag="PLG24_09035"
     CDS             complement(1633..2514)
                     /locus_tag="PLG24_09035"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMZ25077.1"
                     /translation="MRLFLFLPIVALLMTSCAKPLADFAVNSSEKKVTAPIHFDNKSK
                     KATTFHWDFGDGKTSADPSPTHIYYHSGRHEVTLKASDGKKSTIKKMEINIEPPTHCE
                     VLIETEFGNMLVQLSDATPLHRDNFIKLAEQGFYDGLAFHRVIRNFMIQGGDPNSKDE
                     NSQALGSGGPGYTIPAEFVDTLFHLRGAIAAARTGGPMNPEKKSSGSQFYIVQGDQVD
                     DAYLNAREASGKVRYPTSVKEAYKAMGGTPHLDGEYTVFGKVIQGLDVIDKIASQQTG
                     PGDRPVKPIRMKVIVIK"
     gene            2582..4795
                     /locus_tag="PLG24_09040"
     CDS             2582..4795
                     /locus_tag="PLG24_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HMZ25078.1"
                     /translation="MFRSLIFSLLFFVFSLPVFAGEGMWLPILLSQLNEKEMQSKGMK
                     MTAADIYSVNQGSLKDAIVQFGGGCTGEVISTTGLLLTNHHCGYGQIQRHSTLENNYV
                     KNGFWAKSKSQELSNPGLTVTFIVSMADVTKDILSGVSPEMSPADKQSKIDKNMESLK
                     KNYPHESYQDILVRSFYHGNQYYLFVTEVYKDVRLVAAPPASIGNFGVDTDNWVWPRH
                     TCDFSLFRIYADKNNKPAEYSPDNVPFTPRHFLPISLDGVKEGDFTLVFGFPGRTNEY
                     LPSESIRQITDLSDPTKVAIRTTALDIMHKYMMGDEGTKIKYVTKAAGIANGWKKWQG
                     EMEGLKKTDAYGKKQKYEQEYKRLLNANQDLKSEYGDVLENLNANILKANEYVKIREY
                     SIELLRTNIEIMRNYSRLDAIVNAFEKNGASEAMKKADALKSWSTGYFKDYVRAIDEE
                     IFAALFKMYEENVDAEYHFPRLKDIAPGKEGYISRIKLLFDGSPLASQDQLEQLLEKK
                     PEDMINSIKGDGLYLFYREMLDQYNAKVAPNVTKYDEAIDQGMQKYMAAQMTVFKDKR
                     FYPDANSTLRITYGNVNGYTPRDAVKYEFQTWLDGVMEKYIPGNYEFDLPQKLIDLYE
                     KKDFGPYAENGKLPVCFIASNHTTGGNSGSPAIDANGNLIGLNFDRVWEGTMSDYNYD
                     ASICRNIMVDIRYVLFILDKYGDCKHLVDEMKLVHPKAPVKKAQPMPKSSNKVKK"
BASE COUNT         1573 a         1087 c         1095 g         1444 t
ORIGIN      
        1 ggtcaatact atgacggttc cgtgttgatt ggcagcgaag ccgaaagtct gctctgccat
       61 gccagccaca tcagcatcat tggccacgaa aacccggctt ccggtatgtc tgcccagcac
      121 ttcctgtaca tcagtgttaa gccaactttg gtcgatattt gaagctgaca ttgccactcc
      181 gtttttaatt atcgccggga agccacatcc aataattccc tcaaacccga atttatgctt
      241 tatttctgca gctaccttcc cgactgcctc aggtgtagaa ggctcgggag tcataatttt
      301 tactcgttct gtaagtagtt ttccggtctt cacctcaatg actgcccctt taatgccggt
      361 tccaccaata tccaggccta gaatatgctc tttttccatt tcaattcatt cagtgtatct
      421 ataaaaccgt tttaattcga agcaatcaat taaaaaatcc aaggagaaac aatgcagctt
      481 gcatgtatcc ggacaacttg cctatttata tcaaaatttc tcagccgtat caagcacggc
      541 ccattgcctt cagaaagcgt atatgagcca tgatggccga ccacatggtt ctgtattcga
      601 tgtatttgat ctggtattcg tcacacactt ttctaacaat cttgctgatt cccggatagt
      661 ggatgtgaga aattttgggg aatagatggt gttctacctg aaagtttaac ccacctacca
      721 gccaacttat ggtcttgctt ttagttccaa agtttgcggt agttttcaat tgatgagtag
      781 cgaattcatc atcaaattta ttggtatctt cattagccaa aggaaaggaa gtatcctcta
      841 cggtatgggc caactggaaa acaatgctca ggcaaaatcc agcggtgagg ctcataatta
      901 taaatccaag caaccatggc cagaagccga aaatatatat tggcaagaca caaaagaaca
      961 aggcatgcca gatcttgacc agccaaaatc tgaaatgatc actcattttc atatttgtaa
     1021 tcgggaattc gcctattttc attttgaaat actttttata atctgctgca aagacccaat
     1081 acaaatacat gaccatatac aaggcaggaa aatagatatg ctgatacttg tggaatggca
     1141 atcgggtctg gcttggagca agtctcatca atttgcccac ctcgatatcg tcatccagat
     1201 catcgacatt ggtataggta tgatggatca cattatgctt gatattccag aagaaatggc
     1261 tggcgcccaa catactcatg gtgtaactgg caagacgatt gctactctga tgggtagaaa
     1321 aactaccatg gctaccatca tgcatgatat tgaaacctat tgttgctacc aaaaggccca
     1381 acaggataca ttcggaatag gcaatagccc aatggggctg gaatataaac aaatggccaa
     1441 cggtaagtgc gaataaagtg agtaaaattg ctgctttcca gtaaagattg gcattgccgg
     1501 tctgtcgaat tttgttttct tcaaaataca ggctaacacg gcgtcttaat tcactgtgga
     1561 gggatggtct gacttgagga aatcttggaa atgctttggt cgaagccatt atgctattat
     1621 ttatttgatt gattacttaa ttacgatgac tttcatacga attggtttaa caggtcggtc
     1681 accggggcct gtttgttggg atgcgatctt atcaattaca tcaagtccct gaattacctt
     1741 gccgaaaacg gtgtattccc cgtccaaatg aggtgtgccc cccatggcct tgtaagcttc
     1801 cttgacggaa gtagggtacc tgaccttgcc ggaggcctct ctagcgttca ggtaggcatc
     1861 atcgacctga tcaccctgaa caatgtagaa ttgagatcca ctggattttt tctccggatt
     1921 catagggcca cctgtccttg ctgccgcaat agcgcctctc aggtgaaaca gagtatccac
     1981 gaattcagcg ggtattgtgt atcccggacc gccactacct aatgcctggc tgttttcatc
     2041 cttggaatta gggtctccac cctggatcat aaaattcctg attaccctgt gaaaggccaa
     2101 tccgtcataa aatccctgtt cggccaattt gatgaaatta tcacggtgaa ggggtgtcgc
     2161 gtcagacagc tggacaagca tattgccaaa ttctgtctca ataagtacct cgcaatgcgt
     2221 aggcggttcg atattaatct ccatcttttt aattgttgat ttttttccat cagaagcttt
     2281 cagtgtaact tcatgccggc cactgtgata atagatatgt gtaggagagg gatctgccga
     2341 agtcttgcca tcaccgaaat cccaatgaaa agtagtggct ttttttgatt tattatcaaa
     2401 atgaatagga gcagtcactt tcttttcgga agaattgact gcgaagtctg ccaaaggctt
     2461 tgcacagctt gtcatcagca atgccacaat aggcaaaaaa agaaatagtc gcatttattc
     2521 cgtaaatttt gcgctaaaat acagtcattt tactaagttt gagcaaaata aaatattaaa
     2581 tatgttcagg tctttaattt tctcactatt attttttgtc ttcagccttc cggtcttcgc
     2641 gggagaaggg atgtggctgc ctattttact atctcagctc aatgaaaagg aaatgcaatc
     2701 aaagggcatg aagatgactg ctgccgatat atacagtgtc aaccaaggaa gcctcaagga
     2761 cgccatcgtt caatttggcg gggggtgcac cggagaagtc atttccacta ccggcttgtt
     2821 attgaccaat catcattgcg gttatggcca gatacaaagg cattcaaccc ttgaaaacaa
     2881 ctatgtcaaa aatggattct gggcaaaatc aaagtcacag gagctctcca atcccggtct
     2941 taccgttaca ttcatcgttt caatggcgga tgtaactaaa gacatattaa gcggcgtgag
     3001 tccggagatg agtccggcag acaagcaaag caagattgat aagaatatgg aaagtcttaa
     3061 aaaaaattat cctcatgaga gctatcagga tatattggta cgttcgtttt accatggcaa
     3121 tcaatattat cttttcgtca cagaagtgta taaggatgtt cgattagttg ccgcaccacc
     3181 tgcctccatc ggaaattttg gtgttgacac agacaattgg gtttggccaa ggcatacatg
     3241 tgatttttcc ctattcagaa tctatgctga taagaacaat aaacctgctg aatactcccc
     3301 ggacaatgtc cctttcacac cgcgtcattt cctcccgata tcattggatg gagtaaagga
     3361 gggtgacttt accctggtat ttggattccc cggcaggacc aatgagtatc tgccatctga
     3421 atctatccgc cagattaccg accttagtga cccgacaaaa gtcgcaatcc gaactacggc
     3481 ccttgacatc atgcataaat atatgatggg ggatgaaggc accaagataa aatatgtcac
     3541 caaggctgcc gggatagcca acggctggaa aaagtggcaa ggtgaaatgg agggtctaaa
     3601 aaaaaccgat gcatatggca aaaagcaaaa atatgagcag gaatacaaaa ggttattgaa
     3661 cgccaatcaa gatctgaaat cagaatatgg cgatgtgctt gaaaatctga atgcgaatat
     3721 actaaaggct aatgaatatg tcaagattcg ggaatacagt atagaactac ttcgtaccaa
     3781 catcgagatc atgcgcaatt attcaaggct ggatgccata gtcaatgcct ttgaaaaaaa
     3841 tggagcctcg gaggcaatga agaaagctga tgctttaaaa tcatggagta cgggatattt
     3901 caaggattat gtgcgtgcta tcgatgaaga aatctttgca gcccttttca agatgtatga
     3961 agaaaatgtg gatgcggagt atcattttcc cagattgaaa gacatagcac cgggcaaaga
     4021 gggttatatc agcagaatca aattactttt tgacggatca ccgctcgctt cgcaggatca
     4081 attggaacaa ctgttggaga aaaaaccgga agatatgatc aatagcataa agggagacgg
     4141 gctctatctt ttttacagag agatgcttga tcaatacaac gccaaggtag cacccaatgt
     4201 aaccaaatac gatgaggcga tcgaccaagg catgcaaaaa tacatggctg ctcaaatgac
     4261 tgttttcaag gacaaacgat tttatccgga tgccaattca accttacgaa tcacttatgg
     4321 aaacgtaaat ggatacacac cgagggatgc cgtaaaatac gaattccaaa cctggttaga
     4381 cggtgtaatg gaaaaatata taccgggtaa ttatgaattt gacctgccac agaaacttat
     4441 tgatctgtat gaaaaaaagg acttcggccc ttatgccgaa aatggcaaat taccagtttg
     4501 ctttattgca tccaatcata cgaccggtgg caatagcggc agtcctgcca tcgatgcgaa
     4561 tggcaacctg ataggcctta actttgaccg ggtatgggaa ggtactatga gtgactataa
     4621 ttatgatgct tcaatttgca ggaatatcat ggtcgatatc cgatatgtat tgtttatctt
     4681 agacaaatat ggagattgta aacatctggt ggatgaaatg aagctggttc accccaaggc
     4741 accagtaaaa aaagctcaac cgatgcctaa gtcttctaat aaagtaaaga aatgatccgt
     4801 tgacatttta attctttctt taaaaacagg tatcagacaa ttgtgatacc tgtttttgtt
     4861 tttgtaataa ccactgaaac tgatcaggtc catgaagtct tgaaacacgg tgatttgaac
     4921 cccgggtatc atatcggtga caagcccagg ttaccttcag gcagacgata ttctgcgcag
     4981 attgggactc accgtaaggc aatgtacaaa aatgtgtttc cactcctcta actctcatgg
     5041 attgccaaag ttgttcttga aagacctaca aatctatgct taaagattaa ctgccaccta
     5101 aaattcatct tataccaaga tgatttatat aatagtccat aaatttttgg tataataccg
     5161 aggttacagt cgtatagacc aatgaaattg ttctataac
//
LOCUS       DAOQQN010000232         5186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7272_length_5186_cov_4.258624, whole genome shotgun sequence.
ACCESSION   DAOQQN010000232 DAOQQN010000000
VERSION     DAOQQN010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7272_length_5186_cov_4.258624"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1674
                     /locus_tag="PLG24_09045"
     CDS             <1..1674
                     /locus_tag="PLG24_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25079.1"
                     /translation="LIDKPGKADDGLDKDYAFSWSLSIPESMTHVIPNFMGGESSSFF
                     VSDPESHSLRALQNMNNQELAQQLAQTTSKYFGQQPFTAGAWYWGITVVLFFFLGFFS
                     LRSWIRWWGLCSLIFLVLLSWGNHFSALNYFLFDYFPLFNKFRAVSMTINLAHLVLML
                     IGFLGLREFLTMDQAGRMKALKMTMIPAGVILILGFYQGWLHTLTSPIDEKLAKYPEL
                     ISALYQDRAVAVRGDVWRTFFFIAAFFGLLYLFVKYQWNNVVALSVLAALIFIDLVTV
                     DKRYLPNSKFVMQSDLNASEEPRPVDKEIMQDKQLSYRVADFTSNMFNDAFLSNFHKN
                     VGGYHAAKLAIYQDVIDKYLSNPRDYFHVYGMLNTKYIIGSGADNRLFAQPNPEAMGN
                     AWFAREVKWVDTPQQELDALGDTANIHNAVVNKSFKSSIKSDSYTPDPAAKIEMTKYI
                     PDHMTYTYEAASPQFVVFSEVYYPEKKGWHVYIDGKRKEGLVRTNYVLRGLEVPEGKH
                     TLEMKFEPESYYAGQKFGRIGSILLLLLLAGAIVTEFRDEKNKDKNVKE"
     gene            1678..3174
                     /locus_tag="PLG24_09050"
     CDS             1678..3174
                     /locus_tag="PLG24_09050"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HMZ25080.1"
                     /translation="MFNSPGKQSVVRILISVAGYIIGALSTLFIYPLDMKLHGLSTFL
                     LTASLMFAPFILVGSGAVAINNYNEFKTEDKRDNGFLGVIISMYLLGSLIFALLFFVG
                     GDWLSHFFESRDADYNKYFMYILPLTILQAGCALIDVYLNNFTKVIIPALFKEFLLKL
                     TLPLLIGLHILGYISIEHVVWGLVGNYVLALVLLLIYTAYLRKESFVINFSFLTGDRV
                     RRIMHYAFFAILSAFGNQLVLYIDTVMIKAILPDFKYVSIYNMHSSISNFINIPVIAI
                     ISVASPIVARYLSENDMGKLAGLYRSTSSIMLLLGTFLLMGILINMESVYAFMSNGDA
                     YSLGFMIIVFLGLTKIVDMGTGLNGQIIGYSQYYRINFYTLIVLGLSNAILNYFLIHT
                     MGVTGAALATLISMVLYNLFKLYYVRKKFGIWPFSNKDILIIMFAILSALAAYMAPNT
                     GNPYIQILIRTTLFTVLFWAAALYFNLSPRVTIIIKDNWKKFRKGLGK"
     gene            3409..4944
                     /locus_tag="PLG24_09055"
     CDS             3409..4944
                     /locus_tag="PLG24_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HMZ25081.1"
                     /translation="MISGTQKNIQKEIMRSLSVFILLMNVTTYSFTQCLSGDCEDGQG
                     VLKFKSGTVYIGEFSGGEFDGTGIMISANGDRFFGKWKDDMRTGYGKLLIGGGEQRYE
                     GEFRNNKFEGYGTFQYRDKSIYTGMWESSSVNGPGRFRTSGGDIVEGIWQNGNLTHRQ
                     DITELMKGKSKKLRDCNLSNCNSGIGIFNYPDGSRWEGNMRNGKPTRLGVCYYSNGDI
                     YLGEWLDDRPQGYGVFYYNDGKKIESFWEAGKAVQLTEPQITESPITQTQFDKQVKIY
                     SVIIGIGRYPDLPMLKYTDDDAYLLYSFLKSPEGGAVPDDQVKILIDEAANRENIIKT
                     LKTTFAEADANDVILLYFAGHGINGYYLPFDSDGYKNRISYDEIKRILESSQAKHKLC
                     IADACYSGSLLAQRSAFGESLDLFYSTFEKTKGGTAFLLSSKQEETSLESTGMRQGIF
                     SHYLINGLKGASDSNKDRIITIKELYDYISYQVKKSTAFAQNPVIAGNYDNNMPVGVV
                     RER"
     gene            4941..>5186
                     /locus_tag="PLG24_09060"
     CDS             4941..>5186
                     /locus_tag="PLG24_09060"
                     /inference="COORDINATES: protein motif:HMM:NF040143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HMZ25082.1"
                     /translation="MNATNEIHFLGIRHHGPGCAKSVQAFLDEYKPDLILLEAPENTQ
                     ELLEKTDISTFKLPVAILLFKEGEAENNYVLPFAKFSP"
BASE COUNT         1514 a         1053 c         1075 g         1544 t
ORIGIN      
        1 ctaatagaca aaccaggcaa ggcagatgat ggtcttgata aggactatgc tttcagctgg
       61 tcactcagca tcccggaatc catgacacac gtaatcccca atttcatggg cggagaaagc
      121 tcttctttct ttgtcagtga ccccgaatct cactccctgc gtgccttgca gaacatgaac
      181 aatcaggaac tggcacagca actggctcag actacttcaa aatactttgg acaacaaccc
      241 tttactgctg gtgcctggta ttggggcatt actgtagttt tatttttctt ccttggattc
      301 tttagcttga gaagctggat tcgatggtgg ggcctttgct ccctgatatt tttagtctta
      361 ttgagctggg gtaatcattt ttcagcctta aattacttcc ttttcgatta tttcccgctc
      421 ttcaataagt tccgggcagt ctcaatgacc atcaatctgg cccatctggt cctgatgctt
      481 atcgggttcc tcggcctcag agagtttctg acgatggatc aggcaggcag aatgaaagcg
      541 cttaagatga caatgattcc ggccggagtt attcttattc ttggttttta tcaaggatgg
      601 ttacataccc taacctcacc gatagatgaa aaattagcaa agtatcctga attaatttcc
      661 gcattgtatc aggacagggc agttgccgtc agaggtgatg tatggcgtac attcttcttt
      721 atcgcagctt tctttggttt gctatatctg ttcgtaaagt atcaatggaa taatgtagtt
      781 gccctctctg tcttagctgc tttaattttc attgatctgg tgactgtgga caagcgctat
      841 ctgcctaatt caaaatttgt aatgcaatct gatttaaatg cttctgaaga accgaggccg
      901 gtggataaag aaattatgca ggacaaacaa ttaagttacc gcgtcgctga ctttacttca
      961 aatatgttca atgatgcctt tcttagcaac ttccataaaa atgtaggtgg atatcacgct
     1021 gcaaaactag ccatctatca agatgtgatt gacaaatatc ttagtaaccc aagagattat
     1081 ttccatgtat atggtatgtt gaacacgaaa tacatcattg gaagtggtgc cgacaatcga
     1141 ctttttgcac aaccaaatcc cgaagcgatg ggtaatgcat ggtttgcacg tgaagtaaag
     1201 tgggtggaca cacctcagca ggaacttgat gcattaggcg acactgccaa tattcacaat
     1261 gctgtggtaa ataagtcttt caaatcttcc ataaaatcag acagttatac gcctgatcct
     1321 gcagcaaaga ttgagatgac caaatacatt ccggatcata tgacttatac ctacgaagct
     1381 gcatcgccac aattcgtagt cttttccgaa gtctattatc ctgagaagaa aggatggcat
     1441 gtgtatatcg atggtaagcg caaggaagga ttagtgcgta ccaattatgt gctgagagga
     1501 cttgaagtgc ctgaaggcaa acatacactg gagatgaaat tcgaaccgga atcctattat
     1561 gcagggcaga aatttggccg gatcggttcc atccttctgc ttctattgct tgcaggggca
     1621 attgtaacgg aattcagaga tgagaaaaat aaggacaaaa acgtaaagga ataacttttg
     1681 tttaattctc cgggtaagca gtcggtcgta aggatattaa tcagtgtagc aggctacatt
     1741 attggagccc tgagcacgct atttatttat cctttggata tgaagcttca cggcctcagc
     1801 acctttctgc tgactgcctc tctgatgttt gcaccattca tcttagtcgg ttcgggtgct
     1861 gtagccatca ataattataa cgagttcaag acagaagaca agcgggacaa tggtttcctg
     1921 ggtgtaatca tatccatgta tctgttaggc agcttaatat ttgctttgtt attctttgtt
     1981 ggtggtgact ggctcagtca tttttttgaa tccagggacg ccgactacaa caagtatttt
     2041 atgtacatcc tgccactcac aatattacag gcaggatgcg cattgattga tgtttatctc
     2101 aacaatttta ccaaggtcat cattccggca ctgttcaaag aatttttact gaagctgacg
     2161 ctgccgttgt taataggcct gcatattctt ggttatatct caatagagca tgttgtatgg
     2221 ggtttagtgg gtaattatgt cctggcattg gttttactat tgatttatac cgcctatctc
     2281 aggaaagaga gctttgttat caattttagc ttcctcacgg gtgatcgtgt aagacgaatc
     2341 atgcattacg ccttctttgc cattctgagc gcttttggca atcaattggt actgtatatc
     2401 gacaccgtca tgatcaaggc cattctaccg gacttcaagt atgtttccat atacaacatg
     2461 cattcgagca tatccaattt tatcaatata cccgtaatag ctatcatttc agttgccagt
     2521 ccgattgtgg cgcgttatct ttccgaaaat gatatgggta agctggcagg cctgtatcgt
     2581 tcgacctctt ctattatgtt attgcttgga accttcctgc tcatgggaat tctgatcaat
     2641 atggagtcgg tgtatgcctt tatgagcaat ggagatgctt acagcttagg gtttatgatt
     2701 attgtgtttc ttggactaac aaagatcgtg gatatgggca caggtcttaa tgggcagatc
     2761 atagggtaca gccaatatta tcggatcaat ttttacaccc tgattgtact gggcctctcc
     2821 aacgcaatcc tgaattactt tcttatccac accatgggcg tgacgggagc tgctttggcg
     2881 accttgattt caatggtcct ttacaatctc ttcaagttgt actatgtcag gaaaaaattt
     2941 ggaatttggc ctttttcaaa caaagatatc ctcatcataa tgtttgcaat cctgtctgca
     3001 ttagcggcct atatggctcc aaatacagga aatccctata tccaaattct cattcgaact
     3061 acgctattta ccgttctttt ctgggctgca gcgttgtact ttaatttatc tccccgtgtg
     3121 acaatcatta ttaaagataa ttggaagaaa tttagaaaag gtttaggcaa atgattccct
     3181 ccaaaaaata tttattccct attctaaaat ttgaatatca gatcattatt aacatcaaat
     3241 accggcattc atactaatct tcaaccaata caatgtatat gccattctcc catacccaca
     3301 atatttcaat tcatcaaaaa cgttattatg tcactggtta agcatctact ttaaatcagc
     3361 aactttttct accactctat cgttcattaa cattataatt attccaatat gatctccggt
     3421 acgcagaaga atatacaaaa agaaatcatg agatccttgt ctgtttttat cctgttaatg
     3481 aatgtcacca cttattcatt tacgcagtgc ctatcaggtg attgtgagga tggtcaggga
     3541 gtcttgaaat ttaaaagcgg aactgtctat ataggagaat tttccggtgg agaatttgat
     3601 ggaaccggca tcatgatatc agccaatggt gatcgatttt tcggcaaatg gaaagacgat
     3661 atgcgaaccg gttatggcaa gctcctgata ggaggaggag aacaaagata tgaaggagaa
     3721 ttccgcaata acaagtttga agggtacgga acctttcaat acagagataa atcgatttac
     3781 acaggcatgt gggagtcatc atctgtcaat ggccccggtc gtttccgcac ttctggcgga
     3841 gacattgtag aagggatttg gcaaaacggc aatcttaccc atcgtcagga tattacagaa
     3901 ttgatgaagg gtaagtccaa gaaactacgt gattgtaacc tatcaaactg caattcaggc
     3961 ataggaattt tcaactatcc ggacggatca agatgggaag gcaatatgcg aaatggtaag
     4021 cccaccagat taggtgtttg ctattattct aatggtgaca tctatctggg agaatggttg
     4081 gacgacagac ctcagggata tggtgtattt tattataatg atggaaagaa aatagaatcc
     4141 ttttgggaag cagggaaagc agttcaatta acagaaccac aaataactga aagtccaatc
     4201 acgcaaaccc aattcgataa gcaagtcaaa atatattcag taatcatagg aattggccga
     4261 tatccagatc taccgatgct gaaatatacc gatgatgacg cttacctgct gtattcattt
     4321 ctcaaaagcc ctgaaggtgg tgctgttcct gacgatcagg tcaaaatact tattgatgaa
     4381 gcagccaaca gagagaatat catcaaaaca ttaaagacaa ccttcgccga agcagatgca
     4441 aatgatgtca tcctgttgta tttcgcaggt cacggcatta atggatatta tctccctttt
     4501 gattccgacg gttataaaaa tcgtatcagc tatgacgaaa taaagagaat actcgaatcc
     4561 agtcaagcta aacataaact ttgtatagca gatgcctgtt attccggtag tcttttggca
     4621 cagagaagtg ctttcggcga atccctcgat ctgttttatt caacatttga gaaaacgaaa
     4681 ggcggaactg ccttcctgct atcttcaaaa caagaagaaa catccctgga atccacggga
     4741 atgcgtcagg gtatattcag ccattacctc attaacgggc tgaaaggcgc ttctgattca
     4801 aataaagaca gaataatcac gatcaaagaa ctgtatgact atatcagcta tcaagtaaaa
     4861 aaatctacag catttgccca aaatccggtt atagccggca attatgacaa caacatgcct
     4921 gtaggtgtag ttagggaaag atgaatgcaa ccaatgaaat acacttcctc ggcatccgtc
     4981 atcatggtcc aggctgtgcg aagtctgtac aggcatttct ggacgaatac aagccggacc
     5041 tgatcctatt ggaagcgccg gaaaatacgc aggaattact tgaaaagacg gatatttcaa
     5101 cctttaaatt gcctgttgct atcttattgt tcaaggaggg agaggccgaa aacaactacg
     5161 tattaccatt tgccaaattt tctccc
//
LOCUS       DAOQQN010000233         5159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7323_length_5159_cov_5.389303, whole genome shotgun sequence.
ACCESSION   DAOQQN010000233 DAOQQN010000000
VERSION     DAOQQN010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7323_length_5159_cov_5.389303"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            464..3316
                     /gene="uvrA"
                     /locus_tag="PLG24_09065"
     CDS             464..3316
                     /gene="uvrA"
                     /locus_tag="PLG24_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HMZ25083.1"
                     /translation="MPRTKKQISPEGEPVILYDKLNIVIKGARTNNLKNIDLTIPKHK
                     LIVVTGVSGSGKSSITMDTLYAEGQRRYVESLSSYARQFMTRIKKPEVDYIKGITPAI
                     AIHQKVSSANARSTVGTLTEIFDYLRLLYARIGKTISPITGKVIKKDSVSDIVNFLNN
                     KEEGTRFSILSPFSYNEAEEPLRTVLEKLEQKGFNRVWLDGEVQRIDELVSNLPQGIN
                     KDSENNKIVAYVVVDRIAVRKEDVENFKRIADSIETAIAESNGSCVLLTEEGEMHHFS
                     TRFEENGIGYEEPSVALFNFNSSVGACHVCEGYGMAIGIDESKVIPNKHLSLYDHAIS
                     PWSGDKGKNFYKSMIQNAERLGLSIHTPYHKLSSRELEIIWRGGDGFLGIDDYFKKLQ
                     NTSYKIQDRIALARYRGRTICPECRGKRLRKEALYVKVNGRHIAELAEMDIEQLDEFF
                     REIRLEERDVTIAERILSEINQRLSTMVAIGLGYLTLQRPASTLSGGETQRIHLTRLL
                     GSNLTGSLYLLDEPSVGLHPRDTRNLVTALKKLRDLGNTVIVVEHEEEVIRSADYLVD
                     MGPEAGVLGGEVVFTGNYKDIAQIPVNNSLTIDYLLGRKKIEVPKERRVGRNIIRLDG
                     VYLHNLDGVNVSIPLHSIVCVTGVSGSGKSSLVRGALYPTLVNAIQTRSAHTSNNIVR
                     DLYGDFHTINVVEMVDQQPIGKSSRSNPITYVKAYDYIRDLFTAQPLSKLRGFKPKHF
                     SFNVEGGRCEICKGEGVTIVDMQFLADVSLVCDECKGRRFKNEVLDIKYKDKNIYDVL
                     EMDIREAVDFFADQPEIINRIQPLLDVGLGYVKLGQASSSLSGGEAQRVKLASFLTKD
                     NKVPHTLFIFDEPTTGLHFEDVRKLLIAFNKLIDRGNSIIIVEHNMEVIKSSDYIIDI
                     GPEGGRNGGKILYQGPPEGLKAVKDSHTATFLDLEN"
     gene            complement(3373..4119)
                     /locus_tag="PLG24_09070"
     CDS             complement(3373..4119)
                     /locus_tag="PLG24_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HMZ25084.1"
                     /translation="MKLKLKVWRQKNGQSEGAFQNYDVDDVSPDMSFLEMFDVLNERL
                     INEGGDPIAFDHDCREGICGMCSMYINGQPHGPNRGVTTCQLHMRSFNDGESITVEPW
                     RAKAFPVIKDLVVDRTAFDRIIAAGGFISSNTGNAQDANSIPIGKEIADKAFESAACI
                     GCGACVAACPNSSASLFTAAKISQLNRLPQGQPERYIRTRDMVHQMDKEGFGACSNTG
                     ACEAECPKEISISHIAKMNRDYIKASLFKD"
     gene            complement(4171..>5159)
                     /locus_tag="PLG24_09075"
     CDS             complement(4171..>5159)
                     /locus_tag="PLG24_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HMZ25085.1"
                     /translation="DVASRAAKERCDEGYGVGTSKMAVYLDFASAIKRYGAIEVTKHG
                     IANPTENDIIHHGKEIIKEKYGNLFDMYKQITGENPYEVPMRIYPAVHYTMGGLWVDY
                     NLMTTVPGMYALGECNFSDHGANRLGASALMQGLADGYFVIPYTIGDYLSSEIRTKAI
                     STDHEAFVAAEADVRARIEKLMNVKGKHSVDHFHKKLGKVMWNKCGMARNAQGLKEAI
                     EEIRQIREEFWSDVRIPGAPDEFNPELEKAGRVADFLELSELMCIDALNRNESCGGHF
                     REEFQTEEGEALRDDENFSYVSAWEFKKDGDFSLHKEPLTFEYIKVAQRSYK"
BASE COUNT         1459 a         1115 c         1153 g         1432 t
ORIGIN      
        1 gcggtttttg aattattact ttcctaccta cttcgatttt gggggatggg ggtgatgccc
       61 acctctgaag taggtagatg attaatagtc gccttttcaa ttaccattgg cctttccagc
      121 aattttatga ataaaagcga aaccaaatcg cgtattttaa ttttctcagc atgatgaaac
      181 atcggtgcca tcataatatt cagttattat ttttctacct acttcatttt ttcgaccctg
      241 acttcaaagc attaatctta tacatcacaa cacttaatct cttacactga aaccatttgt
      301 catcatggtt tgaaatttta atctgtgtag ctggtattaa ctttttttac atgtcttgtg
      361 ttatgactaa ccatgatgct ctttatttga gtttgatacg tgatgtcaaa attcactact
      421 tttacaactt ttattctttc aaatccatat agggaaccaa aatatgccaa ggacaaaaaa
      481 acaaatttca cctgaaggcg agccggtgat tctttacgat aagttgaata tcgtaattaa
      541 gggtgcacgg accaacaacc tgaagaatat agacttgacc atacccaagc ataaactcat
      601 cgttgtgacc ggtgtgtcag gtagcggtaa atcatccatc actatggata cgctctatgc
      661 ggaaggacag cggcgttatg tagagagcct atccagctat gcccgtcaat ttatgacacg
      721 aatcaaaaag cctgaggtgg actatatcaa aggcatcact cccgccatag ccatccatca
      781 gaaggtaagc agtgccaatg caaggagtac ggtaggtacc ctgaccgaga tcttcgacta
      841 tctgaggctt ttgtacgcaa gaattgggaa aacaatttca cctatcactg gcaaggtaat
      901 taaaaaagat agcgtcagtg acatcgtgaa ttttctcaac aacaaggaag aaggcactcg
      961 attttctatt ttgagtccat tttcctacaa cgaagcggaa gaacccctga ggactgttct
     1021 cgaaaagctg gagcaaaaag gattcaacag ggtgtggctt gatggagaag tccaaagaat
     1081 agatgaactt gtgtcaaacc tgcctcaagg aataaataag gacagtgaaa ataataaaat
     1141 tgtcgcttat gttgtggtgg acagaatcgc agtgcgaaag gaggatgtgg aaaatttcaa
     1201 gcgtatagca gattctatag aaacagcaat tgcagagagt aatggcagct gtgtcctgct
     1261 cacagaagaa ggtgagatgc atcattttag tacgagattt gaagagaatg gtatcggtta
     1321 tgaagagcct tcagtggcct tgttcaattt caacagctca gtaggcgcct gtcatgtatg
     1381 tgaaggttat ggaatggcca taggtatcga tgaatccaag gtcataccca ataaacatct
     1441 tagtctgtat gaccacgcaa tctctccatg gtcaggcgat aaaggtaaaa acttttacaa
     1501 gtcgatgatc cagaatgcgg agcgactggg cctgtccatc catacaccat accacaaatt
     1561 gagttccaga gaattggaga ttatctggag agggggtgat ggatttttgg gcattgacga
     1621 ttatttcaaa aagttacaaa acacgagcta taaaattcag gatcgtatcg ccttggccag
     1681 ataccggggg agaactattt gtccggagtg cagagggaaa agattgcgta aggaggcact
     1741 ttatgttaaa gtcaatgggc gacatattgc tgagcttgca gaaatggata ttgaacagtt
     1801 ggatgaattt tttcgggaaa taagattaga agaacgcgat gtaaccattg cggaacgaat
     1861 actttcagaa atcaatcaac gactgtcgac catggttgcc attggacttg gttatctcac
     1921 attgcaaagg ccggcatcta cattaagtgg cggtgagacg cagcgcatcc acctcacgag
     1981 gctgttggga agtaatctca ccggatcatt gtatctattg gacgaacctt ctgtaggcct
     2041 tcatccccga gatacccgca atcttgtaac tgccttgaag aagcttcgtg atctgggaaa
     2101 tacagtcatt gtagtggaac atgaggaaga agtcatcagg agtgccgatt atctggtcga
     2161 tatgggccct gaagcagggg ttctgggtgg agaggtcgtg tttaccggta attataagga
     2221 tattgcacaa attcccgtta acaacagcct gaccatcgat tatttgcttg gccggaagaa
     2281 aatcgaagtt cccaaagaaa ggcgtgtcgg caggaatatt atcaggttgg atggtgtcta
     2341 tcttcacaat ctggatggag taaatgtctc cattcccttg cattccattg tttgcgtcac
     2401 cggtgtaagt ggtagtggta aaagttcgct tgtcaggggt gcactctatc caacacttgt
     2461 caacgccatt caaaccagga gtgcacatac ctccaacaat attgtgcgcg atctttatgg
     2521 agattttcat accatcaatg tggtggaaat ggtggaccag caaccaatcg ggaagtcgtc
     2581 ccgctccaat ccaatcacct acgtaaaagc ttacgattac atccgtgact tgttcacagc
     2641 acagccatta tcaaaactga gaggattcaa gcctaagcac ttctctttca atgtggaagg
     2701 tggacgatgt gaaatctgta aaggagaggg agtaaccata gtcgatatgc aattccttgc
     2761 cgatgtaagt ctggtctgcg atgaatgtaa aggaaggaga ttcaagaatg aggtactgga
     2821 tatcaaatat aaggacaaaa atatctatga cgtcctcgaa atggatatca gagaggccgt
     2881 tgattttttt gctgatcaac cggaaattat taatcggatt caaccattgc ttgatgtagg
     2941 cttaggatat gtcaaactgg gacaggcgtc atcgagcctt tcgggtggag aagctcagcg
     3001 ggtcaagctg gcaagcttcc tgaccaagga caacaaggtg cctcacaccc tctttatatt
     3061 tgacgagcct accacaggac tccactttga agatgtgaga aaattgctga tagccttcaa
     3121 taagctgatt gatcggggta actctatcat tatagttgaa cataatatgg aggtcatcaa
     3181 aagtagtgac tacatcatcg acatcgggcc tgaaggcgga aggaatgggg ggaagattct
     3241 gtaccaggga cctcctgagg gtttgaaggc ggtaaaggat agccatactg cgactttcct
     3301 ggatctggaa aattaacagg gccgccaaaa gacagccctg ttatctattt tgataatata
     3361 ccaaagatct gtttaatcct tgaacaatga tgccttgata tagtcgcgat tcatctttgc
     3421 gatgtgggaa atggaaatct ctttcggaca ttcggcctca caagcacctg tattggaaca
     3481 tgctccaaac ccttccttat ccatctgatg taccatatcg cgagttcgga tgtatcgctc
     3541 aggctggccc tgtggcaatc gattaagttg gctaatcttg gcagccgtaa ataacgaggc
     3601 actgctattg gggcaggctg ccacacaagc tccacaacct atacaggcag cagattcgaa
     3661 agccttgtca gcgatttcct taccgattgg aatagaattg gcatcttgag cattacctgt
     3721 attggaagat ataaatcctc cggccgcaat aattcgatcg aatgcagttc tgtccacaac
     3781 caggtccttg attaccggaa aagccttagc cctccagggc tcgaccgtga tggattcgcc
     3841 atcgttaaac gaacgcatat gcaactgaca ggtagtcaca cctcggttag gtccgtgtgg
     3901 ctgaccattg atgtacatgc tgcacatccc gcagatacct tcacggcagt cgtggtcaaa
     3961 agcgatagga tcaccacctt catttatcag tctttcattt aaaacgtcaa acatttcgag
     4021 gaatgacata tccggagaca catcatccac atcgtaattc tggaaggcgc cttcagattg
     4081 gccgtttttt tgtctccaga ctttgagttt caatttcata acattcttga tttatattat
     4141 agcaaattgg atttgccggt tgattaaatt ctatttataa cttctctgag cgactttgat
     4201 atattcgaat gtcaatggct ccttatggag tgaaaaatca ccatccttct tgaattccca
     4261 ggctgacaca taggagaaat tctcatcatc cctcaatgct tccccttcct ctgtctggaa
     4321 ttcttcacgg aaatgaccac cacatgattc gttcctgttg agtgcatcga tacacatcag
     4381 ttcacttaat tcgaggaagt cagctacgcg gccagccttt tccagttccg ggttgaattc
     4441 gtccggagct cccggaattc ttacatctga ccagaactct tcccggattt ggcgtatttc
     4501 ttctatagcc tccttgagtc cctgtgcatt gcgtgccata ccacatttgt tccacatcac
     4561 cttgcccaac ttcttgtgga aatgatcaac ggaatgttta cccttaacat tcattaattt
     4621 ttcgatacgc gccctgacat cggcttcagc agcaacaaat gcctcatggt cggtagagat
     4681 tgctttggtg cggatctcgc ttgaaaggta gtcccctatg gtatatggaa tgacaaagta
     4741 accatccgca aggccttgca tcaatgcaga tgctccaagt cggttggcac cgtgatctga
     4801 gaagttacac tctcctaaag cgtacatgcc gggcactgtt gtcatcaggt tataatccac
     4861 ccaaaggcct cccatggtat agtgtactgc cggatagatt ctcatcggta cttcataagg
     4921 gttttcaccg gtaatctgct tgtacatatc gaacagattt ccgtattttt ccttaataat
     4981 ctccttacca tgatgtataa tatcgttttc agtcggattg gcaattccgt gcttggtcac
     5041 ttcgattgcg ccatagcgct tgatagctga agcgaaatca agatatactg ccatcttgga
     5101 agtgccaacg ccatagcctt catcgcaacg ttcttttgct gcacgtgaag cgacgtccc
//
LOCUS       DAOQQN010000234         5134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7383_length_5134_cov_5.677496, whole genome shotgun sequence.
ACCESSION   DAOQQN010000234 DAOQQN010000000
VERSION     DAOQQN010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7383_length_5134_cov_5.677496"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLG24_09080"
     CDS             complement(<1..202)
                     /locus_tag="PLG24_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25086.1"
                     /translation="MRYLQLVLVIFIPAIIFSQPINDDCINAIEITDVANYCSAFGEF
                     NNFGATPSIPSDYICIPNEATAR"
     gene            309..1022
                     /gene="pssA"
                     /locus_tag="PLG24_09085"
     CDS             309..1022
                     /gene="pssA"
                     /locus_tag="PLG24_09085"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HMZ25087.1"
                     /translation="MIRHIPNILTLLNLFCGALAVISVLFLHVEEALVLQAISLIADF
                     LDGLIARKLNVAGPLGVQLDSLADVISFGIFPGAVVYMLLVQSWDIDPGMSDLESRLL
                     LYPAFLLPVFGALRLARFNISTDQGDKFIGMPIPSMSIFFAGLLMIQLNGDIRMIEFI
                     ENKWFLYGSILLFAYLMNSPWIHFKIKPDKKTFSNPFILLLAGAIIGLIIFYPFLAPT
                     IGIVLYSTFSIISHLLKLS"
     gene            1022..1741
                     /gene="rsmI"
                     /locus_tag="PLG24_09090"
     CDS             1022..1741
                     /gene="rsmI"
                     /locus_tag="PLG24_09090"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HMZ25088.1"
                     /translation="MEKEPGRLYVVPTPVGNLGDFTFRAIETLKHVTYIYAEDTRTTG
                     VLLKKYAIDTPLRAFHSFNEHKQIEPILEVVKSGNDIALVSDAGTPGISDPGYLLVHH
                     LIESGVEVTCLPGPTALIPALVMSGLPADKFYFEGFLPHKKGRQTRLQAICDMPCTVI
                     LYESPFRLMKCIEELIEIAGEERPCCIVREISKIHEQAITASLGTLLHKLKSGKIPAK
                     GEIVLVLGGKPNEKKSRSLNK"
     gene            complement(1757..4117)
                     /locus_tag="PLG24_09095"
     CDS             complement(1757..4117)
                     /locus_tag="PLG24_09095"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HMZ25089.1"
                     /translation="MIRCTIIYFFCLLVNQCFSQGPLSVEYIMRDPNWIGVSPSSLRW
                     GADGTLYFKYNPESAEADSLYSLNTNDMPYFSKVSDQSQEVLYEDNLIRNPSDPSMVV
                     YNRQGNIFLRRMKGDVKKIFSTHEYEWATGFSSDGKYLVYKIGNDMFSYNIEKGFIRQ
                     LLAIQSASPRDNKKKLSNQDQVLMEEQKNLFDVFIQEKKVSKKSKEKRDYFVYERGSA
                     DVVGTSISNDLLIAAIRLKDGRTESHTTIMPKYVTERGYTETVNTRAKVGIIQPDYSL
                     KFYNFEKDTLMDFLVNDLPGINAMPNYLEEYPVLHDSLAKASAKKSVNIFDPLWSPNG
                     HLAVVQVYSFDNKDRWIVLFDADSGIMKIIDHQHDEAWIGGPNIGGNYWPGWCVWLDD
                     DHVAFTSEISGYAHLYKYEISSGKIIALTSGNYEVQRVTLAPDRKSLYLLTNQNHPGT
                     KTFVKLALNSLKQIVLLNPDCGLDEVTVSPDGKWIAYLQSTTNQPWELFISENKAGGK
                     STRITDKAISREFGEYPWQSPKVITFRASDGREVYARLYKSRVANPNKPGVIFVHGAG
                     YLQNAHKWWSSYFREYMFHNLLTDLGYTVLDIDYRASSGYGRDWRTAIYRHMGGTDLS
                     DQVDGVKYLKDSAGVNINNIGIYGGSYGGFITLMALFTKPGVFQAGAALRPVTDWMHY
                     NHGYTSNILNVPALDSIAYARSSPINFVDGLKDRLLICHGMVDDNVHFQDVVRLQQVL
                     IDKGKDNWELAAYPMEAHGFKTPSAWTDEYKRILKLFEETINRKSN"
     gene            complement(4234..4518)
                     /locus_tag="PLG24_09100"
     CDS             complement(4234..4518)
                     /locus_tag="PLG24_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HMZ25090.1"
                     /translation="MAERILTLGKHPLSTYKDYIQHASIKEINTIGMSDRMMVKEILQ
                     DMTTLIELEREIMDAASSAGDEGTNDMVNAFMQFKEKSCWMLRAFIGNEK"
     gene            complement(4563..4715)
                     /locus_tag="PLG24_09105"
     CDS             complement(4563..4715)
                     /locus_tag="PLG24_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25091.1"
                     /translation="MEVNEISLSKNQVKPVIDLLNDYLANYQIHYQKLRGAHWNIEGE
                     NFLHCI"
BASE COUNT         1520 a         1102 c          952 g         1560 t
ORIGIN      
        1 cccttgcagt agcttcattg ggaatgcata tataatccga aggaatggaa ggcgtcgccc
       61 caaaattatt gaattcccca aaagccgagc aataattagc cacatccgtt atctcaattg
      121 cattgataca atcatcatta atgggttgag aaaaaattat tgcaggaatg aagatgacca
      181 aaacgagttg caaatatctc atgctaattg aattgaaaac tatactgtgt tatgcaataa
      241 acgaataatt aaccactttt gttagattta ccctttaaat ttgcattttt ccaactaaga
      301 aacagacaat gatcaggcat attcccaata ttttgacctt gctaaatctt ttttgtggag
      361 cattggcagt cataagtgtt ttatttttac atgttgagga agcattggtt cttcaggcaa
      421 tcagcctgat cgctgatttc cttgacgggc ttattgccag aaagctaaat gtggccggtc
      481 ctttgggtgt acaattagac tctttggccg atgtcattag ttttggcata ttcccgggtg
      541 cagtcgtata tatgctgttg gttcaatcct gggatattga ccctggtatg agtgacctgg
      601 aaagccggct tctactctac cctgcttttc tgctgcctgt ctttggagca ttaaggttag
      661 cgcgattcaa tatctcaaca gaccagggtg ataaatttat tggcatgccc atcccgtcca
      721 tgtccatatt ttttgcagga ttgctcatga ttcaattaaa tggggatatt cgaatgatag
      781 aatttataga aaacaaatgg ttcctctatg gaagtatcct gttgtttgcc tatttgatga
      841 acagtccatg gattcatttc aagattaagc cggataagaa gacattcagc aaccctttca
      901 ttttattgct ggcgggcgct atcatcggat tgatcatttt ctatcccttc ctagccccaa
      961 caataggaat cgtactttat tccacatttt ccattatttc ccatctcctt aaactttcat
     1021 aatggaaaag gagcccggta gactttatgt tgttcccact cctgtgggta atttgggaga
     1081 ttttaccttc agggccattg agactctgaa acatgtgact tacatatatg ctgaggacac
     1141 ccgaactaca ggtgttctat taaaaaaata cgcgattgat acacctttgc gtgcctttca
     1201 ttccttcaac gaacacaaac aaatagaacc aattctcgaa gtagtgaaaa gtggaaatga
     1261 tatagctctg gtttctgatg ccggaactcc cggaatatca gaccccggat atctgttggt
     1321 tcatcatctt atagagtccg gagtggaagt cacctgctta cccgggccta cagcattaat
     1381 acctgcactt gtcatgagtg gacttcctgc agataaattt tattttgaag gtttcctgcc
     1441 acataaaaag ggacgacaga ccagattgca ggcgatatgt gatatgccct gtacagttat
     1501 cctttatgaa tcgccgtttc ggttaatgaa atgcatagag gaattgatag aaatcgccgg
     1561 agaagaaaga ccctgttgta tcgttcggga aatttccaaa atacatgaac aagccattac
     1621 tgcatcattg ggtacgctcc tccataaatt aaaatcaggc aaaatacctg caaaaggtga
     1681 aatcgtcctt gtattggggg gcaagcccaa tgaaaagaag tcccgcagtc taaataagta
     1741 atatctttac aatggcttaa ttgcttttgc gattgatggt ttcttcaaat aatttaagaa
     1801 tacgtttgta ttcatctgtc caggctgaag gtgttttgaa cccatgcgct tccatcggat
     1861 atgctgctaa ttcccaatta tccttgcctt tatcaatcag cacttgctgt aacctgacaa
     1921 catcctgaaa atgtacatta tcgtcgacca taccatggca aatcagtagt ctgtctttca
     1981 gcccatccac gaagttgata gggctgcttc ttgcataagc aatggaatcc aacgccggaa
     2041 catttaaaat attagaagta tagccatgat tgtaatgcat ccagtcggtc acaggacgaa
     2101 gagctgctcc ggcctgaaaa actccgggct ttgtaaatag agccatcaag gtgataaaac
     2161 caccatacga accaccataa atgccgatat tatttatatt gactccagca ctatccttta
     2221 aatatttgac gccatctacc tgatcagaaa gatcagtccc tcccatatgt ctgtatatcg
     2281 cagttctcca atccctgccg taccctgagc tcgccctata atcaatgtcc agaaccgtat
     2341 aaccaagatc tgtaagaagg ttgtggaaca tatattctct aaaataagag ctccaccatt
     2401 tatgcgcatt ctgaagatat ccggcaccat gcacgaagat gacacctggt ttgttaggat
     2461 ttgcaaccct ggatttgtac aacctggcat agacttccct gccatctgat gccctgaatg
     2521 taatgacttt cggtgactgc catggatact caccaaactc cctacttata gctttgtcag
     2581 tgatccgagt cgatttgcct ccggctttat tttcgctgat gaataattcc cagggctggt
     2641 tagtggtact ttgcagatag gcaatccatt ttccatccgg agatactgtc acttcatcca
     2701 atccgcaatc cggattcaac aaaacaatct gtttcagaga attcaatgcc agttttacaa
     2761 atgtcttcgt accaggatgg ttttgatttg taagtaagta cagactcttc cgatccgggg
     2821 ccaaagtcac ccgttgaacc tcatagttgc cggatgtaag tgcaataatc ttacctgaag
     2881 aaatttcata cttataaagg tgagcatatc cggaaatttc agatgtgaat gctacatggt
     2941 catcatcaag ccaaacacac cagcccggcc aataatttcc acctatattc ggccctccga
     3001 tccaggcctc atcatgctga tgatcaatga tcttcatgat acccgaatcg gcatcaaata
     3061 ggacaatcca cctgtctttg ttatcaaagg aatacacctg aacaacagct aaatggccat
     3121 tgggtgacca aaggggatca aaaatattta ctgatttttt agcagatgct ttagcaaggc
     3181 tatcatgcag gaccggatat tcctcaaggt aattcggcat agcattaatt ccgggtaaat
     3241 catttaccaa aaaatccatt aaagtatcct tctcaaagtt atagaacttc aggctgtaat
     3301 caggctggat aataccaacc ttagcccttg tattaaccgt ttcagtatag cctctttccg
     3361 taacgtattt tggcataatg gtagtatgac tttctgtccg gccatctttt aagcgtatgg
     3421 cagcgataag cagatcatta ctgatggatg tacccacaac atctgcactg cctctttcat
     3481 atacaaaata atcccgtttt tctttactct ttttagagac tttcttttcc tgaataaaga
     3541 catcgaatag attcttttgt tcttccatca acacttgatc ctgattggat agtttctttt
     3601 tattatctcg cggggaagct gattgaatag ccaataactg tcggataaag cctttttcta
     3661 tattataaga aaacatatca tttccgattt tatacactaa atattttccg tccgagctaa
     3721 aacctgtcgc ccactcatat tcatgtgttg agaagatttt ttttacatca cctttcattc
     3781 tccgtaagaa aatatttccc tgacggttgt atacgaccat ggaaggatcc gaggggttcc
     3841 tgatcaaatt atcctcataa agaacctcct gactttgatc cgacactttg gaaaagtatg
     3901 gcatatcgtt ggtattcaag gagtatagag agtccgcttc agcactctcc ggattgtatt
     3961 tgaagtaaag cgtgccatct gcgccccatc tcagcgatga tggtgagact ccaatccagt
     4021 taggatctct catgatgtat tccacagaaa gaggaccctg agaaaaacat tggttgacaa
     4081 gaaggcaaaa aaagtaaata atagtacacc ggatcatgat gaaaaagctt tgtaaggcaa
     4141 gataaggtat aatagattct taataggtga aatatcgaca aaaaagtatt tcataaaaaa
     4201 aaggatggat tcccatcctt ttccggtcag atgttatttc tcatttccaa taaaagctct
     4261 tagcatccaa caactttttt ccttgaattg catgaaggca ttaaccatgt cattggtgcc
     4321 ctcgtctccc gctgaggatg cagcatccat gatttcccgt tccaattcga tcagggtggt
     4381 catatcctgg agtatttcct taaccatcat cctgtcactc attccaattg tattgatttc
     4441 cttgatactg gcatgttgaa tgtaatcttt atatgtactc aaaggatgct tgcccagggt
     4501 caggattctt tcagcaattt catcgatagt tgtctgggca ttggtctaga actcctcaaa
     4561 tttcaaatac agtgtaaaaa attctctcct tcaatattcc agtgagcccc gcgaagcttt
     4621 tgataatgga tttgataatt tgccaaataa tcattcaaca agtcgataac aggcttaacc
     4681 tgattcttac tcaaactgat ctcatttact tccataatag attgatattt ttaaattagt
     4741 tatattagaa tgttacaggt gcgtcatgac aaatgaaagg taccacgacc tgcctctaac
     4801 taattgtaca acaacataat acaagaattg tttaagaaac aacttgaatt actgcaattc
     4861 atcagcgacc tgatatttgt tattataagg aaatgtccaa aagaaggaaa tgcgagaaaa
     4921 attcgacctg aagaatcaat agacattaaa ctttaacgca tataaaaata cgttgtttgt
     4981 gcttataacc cttcaaccaa atggattgac tgaaataaat ggaatcgctt aaaaggtcgt
     5041 ctgatttaaa ttggcgaaga aatgatttac ctccgcctct ctatggaaaa gtacctccct
     5101 tggcttactg ccttcatttt caccgacaat gccc
//
LOCUS       DAOQQN010000235         5104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7453_length_5104_cov_6.457912, whole genome shotgun sequence.
ACCESSION   DAOQQN010000235 DAOQQN010000000
VERSION     DAOQQN010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7453_length_5104_cov_6.457912"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="PLG24_09110"
     CDS             <1..1190
                     /locus_tag="PLG24_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25092.1"
                     /translation="GQAVGSINIEARPELLYSLDQGAHWSEKTHYDKLNAGPYEILIR
                     EGDKCLFSPGFPVEIVSRDSIEYGVIEEKPTCKGMDNGSITLTFPPNIIPEIVWDNNQ
                     HDLTRQGNAGEYTVMMTYGTCAESHTVTIPESDMDNLDWNPHADTVLCTTKEVIYTLP
                     DQYHYEWYIDRIWKDSSAVMSIPDEADIRLLISDSRGCVTVDQWRLDRADEPYDFDFL
                     LPSEGVIHFPVIGIDISKPIPEKVEWTSTSEEREAEKVIANQYHLVYGHPGIYTVEAH
                     YKAGQCEDIVKKQVRIYATKDSLTYPHTGIANRVIASLTLTPNPNTGTFKLIMEYFDI
                     QPGNIWIYNAAGNRVFSRRIEPLVGLEEVPIELPGVRPGVYSLVYLADTGEFHWLHFI
                     IIQ"
     gene            1197..3452
                     /locus_tag="PLG24_09115"
     CDS             1197..3452
                     /locus_tag="PLG24_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25093.1"
                     /translation="MKKNIVPTIFALCFLLLSIDVASGQPYPVTVTFNVIPPAGVSLA
                     EYQVSPTPKVMASIVLTDLQKPVFKVRLKLTIAGNGLLLTTSDQAVLPPVDLLAGQAV
                     TLDQTALAGYFDISTLNITGMNRNEFIRRGQILPEGTYSFCLEAYDYNRPQGLPVSNT
                     GCSIAQVERYDPPILNPPDFTNTILFDMQPPGFQQGLFTWQPMHTGIFPVEYNLRIYK
                     KDGGMESLPDKMILEATPPYISIRTKELLYHLQPTDPPLMPDVQYYGVVQVIPLNYPA
                     IFKNNGNSEKVSFVINPEEKSPCEKPQEYRGAGQRPGIALDWKTYRACEGFVTEYYDL
                     ADEQRKYQDAEIETGDALTDTIRAVVSEHTYILRTGCICLQDTLYTDTIRVHFKRPKA
                     TVPPFSCGSDGGAIAGIPSYLPVLNPDDTIVAADLRVIIRKATGGNGRFSGKGHIEMP
                     YFKYARINVNFTDITVNDEYRMTDGELEVVGVGQNVIGDDALGAIMTILEGMDEWSDI
                     LGEAAAILDMLDQLLLQMAENMPPWLIQEILAIKGMIANPPEGANIDELMARLKELEG
                     ERKVWERLYWEIIIATIERINEDYETNRQQIDENFNTSYNTISPNVIFIQENDIENDP
                     PIDDGLIAEFYADVISSDSITYTIEEYAELNQNIGTNIVTYFSAKKEANIMEIINKLE
                     LELNADFMKVPLFTGLLLKVGKNVISSIKQEFESRNWDLSIIESEEAQIVDKCKAFMI
                     QSIVELYINLN"
     gene            3458..>5104
                     /locus_tag="PLG24_09120"
     CDS             3458..>5104
                     /locus_tag="PLG24_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25094.1"
                     /translation="MKKHKKNLSYILLLSFILIILPMNISANDLNCNGYPSSRSDSTE
                     LNLDKILTEFFAMKKSEFVQQIQRNERIKMGLIDSVGNFVFRLNFEKEIVNYIISKKY
                     DSKKTKFVDSPPDSVFVNMNEAVNNLMLDSIMPGIYYAEFLFATPILWRTEIDNKDAF
                     EILSYLDKNINTTNTKIRKVFEKHVKIFLDEFNDILKNSNSNTNGMFILGHMEFNAEI
                     RDQYKSPAIKTKLFTILKYIKHGTPTNNYNNLLSCIKQDLNENYETNKNTYTRLSVYF
                     QSVADYIKGNLECVGGNNDDEVLSFDCLPKEMNDGNTGIVEIEKSTINNCRKFFVEDK
                     IRVFDDQEYAKINKFGINTFGRLVYVKDNQNKKHFFSKSCTLDQDGKIQKYFSSGRLI
                     NFELIQQQITDKIKLSYGVSYKYVIDQDYQSQTILSGINKQNYLIANLVSEVPCNAGT
                     QVVFINEGQIYVKTLSDQDCNNISDFWANFNVNTPTSDLIQHLLYEKREKTLTVDLSK
                     RVLALDKLIAASCSAYSARNHTLLKMIEAFYPNEYKQVVNK"
BASE COUNT         1628 a          924 c         1159 g         1393 t
ORIGIN      
        1 gtggtcaggc agtgggcagt atcaacattg aagcccggcc ggaattgctg tattctctgg
       61 atcagggggc gcattggtca gaaaagacgc actatgacaa gttgaatgca ggaccgtatg
      121 agatattgat ccgagagggt gataaatgtt tgttctctcc cggatttccg gttgaaatag
      181 tgagccggga cagcattgaa tatggcgtta tagaagaaaa gcctacctgt aagggcatgg
      241 acaatggaag tataaccctg accttcccac cgaatatcat tcctgaaata gtctgggaca
      301 ataaccaaca tgatcttacc cgacagggga atgccggaga atacacggta atgatgacct
      361 atggcacgtg tgcagagagt cacactgtca caattcctga gtctgatatg gacaaccttg
      421 attggaatcc tcatgctgat acggttcttt gtacgacgaa ggaggttatc tataccctgc
      481 ctgaccaata tcactatgag tggtatatag acaggatatg gaaggatagc agtgccgtca
      541 tgtcgatacc cgatgaagct gacataagac tgctcatctc tgacagccgg ggttgtgtta
      601 ccgtcgatca gtggcggctt gacagggcgg atgagcctta cgactttgac ttcctgctgc
      661 cttctgaggg agtgattcat tttcccgtca tcggtatcga tatttcaaaa ccaattcctg
      721 aaaaggtaga atggacatcc acttctgagg aaagagaggc cgaaaaagtc atcgccaatc
      781 aataccatct ggtgtatgga catccgggga tttacacggt agaggcgcac tataaggccg
      841 gacagtgtga agatattgta aaaaaacagg tgcgaattta tgccacgaag gacagtctga
      901 cctatccgca cacaggcatc gccaacaggg tgatagcctc actgacgctg acgcccaatc
      961 ccaataccgg cacattcaag ctcatcatgg aatatttcga tatacagccg ggcaatatct
     1021 ggatctacaa tgccgccggt aatcgtgtat tcagccgtcg tatagagcca ctggtagggc
     1081 tggaggaggt tccgatagaa ttgccgggag tacgcccggg tgtatattca ctggtatatc
     1141 tggcagatac gggcgagttt cattggcttc attttatcat catacaatag gcagatatga
     1201 agaaaaatat agtaccgacg atttttgcac tatgctttct tttactaagc atcgacgtgg
     1261 catcgggaca gccttacccg gtgacggtaa ccttcaatgt aataccgcct gcaggcgtga
     1321 gcctcgccga atatcaggtt agccctacac ctaaggtcat ggcttcgata gtactgaccg
     1381 acttacaaaa gccggtgttc aaggttcgtt tgaagctgac aatagcaggt aacggactct
     1441 tgctcaccac ctctgaccag gcagtgttgc cgcctgtgga cctgctggcc ggacaggcag
     1501 taacactgga tcagacggca ctggcgggat attttgatat atccaccttg aatataaccg
     1561 gtatgaaccg aaatgaattt atcaggaggg ggcagatatt gcctgagggg acatacagtt
     1621 tttgtctgga ggcctatgat tacaacaggc cgcagggtct gcccgtgtcc aataccggat
     1681 gtagcatagc tcaggtggaa agatatgatc cgcccatcct caatcctccc gactttacca
     1741 atacgatctt gtttgacatg caaccgccgg gatttcaaca gggattattc acctggcagc
     1801 cgatgcatac ggggatattc ccggtggagt acaatctgag gatatataag aaagatggcg
     1861 gtatggagtc tcttcccgac aagatgattc tggaagcgac gccaccctat atcagtatcc
     1921 gcacgaagga attgttgtat caccttcagc cgacagatcc gccgctgatg ccggatgtac
     1981 agtactatgg agtcgtgcag gttatcccgc tcaactatcc ggccattttt aaaaacaacg
     2041 gtaattcgga gaaggtatcc tttgtcatca atcctgaaga gaaaagccct tgtgagaaac
     2101 ctcaggagta tcgcggtgcc ggtcagcggc cggggatagc cttagactgg aagacgtacc
     2161 gggcgtgtga aggatttgtc acggagtatt acgacctggc agatgagcaa agaaaatatc
     2221 aggatgcaga gatagagacg ggagatgccc tgacggatac catccgggct gtggtgtccg
     2281 aacataccta catcctccgt accggttgta tctgcctgca ggacacactt tatacggata
     2341 ccatccgggt acattttaaa cggcccaagg ctactgttcc accattttcc tgtggttcgg
     2401 atggaggagc cattgccggc attccgtcgt atttaccggt gttgaatccc gatgatacga
     2461 ttgtggcggc tgatttacgg gttatcatcc gtaaggcgac cggtggcaac ggacgcttct
     2521 ccggcaaggg gcatattgag atgccgtatt tcaagtatgc gcgcatcaat gtcaacttta
     2581 cagacatcac ggtcaatgac gagtatcgga tgacggatgg cgaactggag gtggtcggtg
     2641 tggggcagaa cgtgatcgga gatgatgcct tgggagcaat tatgaccatc ttagaaggca
     2701 tggatgagtg gtcggatata ctgggcgagg cagcggccat actggacatg ctggatcagc
     2761 tgctcttaca gatggctgag aacatgccgc catggctcat tcaggagatt cttgccatca
     2821 agggcatgat agccaatccg cctgaaggcg ccaatataga tgagttgatg gctaggctca
     2881 aggagctgga aggagaaagg aaggtgtggg agagattgta ttgggagata ataatagcaa
     2941 ccatagagag aataaatgaa gactatgaaa ctaataggca acaaattgat gaaaatttta
     3001 atacatcata taataccata agccctaatg taatatttat tcaggaaaat gatattgaaa
     3061 acgatcctcc aatagatgat gggctaatag ctgagtttta tgctgacgtt atttcttcag
     3121 atagtattac ttatacaatt gaggaatatg ctgaattgaa tcagaatata ggtactaaca
     3181 ttgttactta cttttcggca aaaaaggaag ctaatattat ggaaattata aataaactgg
     3241 aactggaatt gaatgctgat tttatgaaag tacctctatt tactggatta ttactaaagg
     3301 ttgggaaaaa tgttatttca agtattaagc aagagtttga atcgagaaac tgggatctta
     3361 gtattatcga aagtgaagaa gctcaaatag ttgataaatg taaagctttc atgattcaat
     3421 caattgttga attatacata aacttaaatt aagagttatg aaaaagcata agaagaatct
     3481 ctcctatata ttattgctct cttttatatt gattattctg ccgatgaata tttctgcaaa
     3541 cgatttaaat tgtaatggat atccaagtag tagaagtgat agtacagagt taaatttaga
     3601 caaaatatta acggaatttt ttgcaatgaa gaaaagcgaa tttgtgcaac agatacaaag
     3661 aaatgagagg ataaaaatgg ggttgattga ttcagttggc aatttcgtct tcagattgaa
     3721 ttttgaaaaa gaaatagtta actatattat ttcaaaaaaa tacgatagta agaaaacgaa
     3781 atttgttgac tcacctcctg attcagtttt tgtcaatatg aatgaagccg tcaacaattt
     3841 gatgttggat tctataatgc ctggcatata ttatgctgaa tttttgtttg ctactccgat
     3901 tttatggcgt acagaaatcg acaataagga tgcatttgaa atcctttctt acctcgataa
     3961 aaatataaat acaactaata ctaaaattag aaaagtcttt gaaaaacatg taaaaatatt
     4021 tttagacgag tttaatgata ttttaaaaaa cagtaattca aataccaatg ggatgtttat
     4081 tttagggcat atggaattca atgctgaaat acgtgatcaa tacaagagtc ctgctattaa
     4141 aacaaagcta tttactatat taaaatacat aaaacatgga accccaacaa acaattataa
     4201 taacttattg agttgcatta aacaggattt aaatgaaaat tatgaaacaa ataaaaatac
     4261 ttatacaagg ctttcagtgt actttcagtc tgttgcagat tatataaagg ggaatttaga
     4321 gtgtgtaggg ggaaataatg atgatgaggt gcttagtttt gattgccttc caaaagaaat
     4381 gaatgacgga aataccggaa ttgttgaaat tgaaaaaagt acaatcaata attgtcgtaa
     4441 attctttgtt gaggacaaga ttagagtatt cgatgatcaa gaatatgcga aaattaataa
     4501 atttggaatt aatacctttg gcagattggt gtatgttaaa gacaatcaaa ataaaaaaca
     4561 cttttttagt aaatcttgta ctttagatca agatggcaaa atccaaaagt atttcagctc
     4621 aggaaggtta ataaattttg agttaattca acaacaaata acagataaaa taaaattatc
     4681 ttatggtgtt agttataaat atgtcataga tcaggattac caaagtcaaa ctattctttc
     4741 cgggataaat aaacagaatt atttgattgc gaatttggtt tctgaagtac catgtaatgc
     4801 aggaacacaa gtagtgttta taaatgaagg tcaaatatat gtaaaaacac tttccgatca
     4861 ggattgtaat aatatttctg atttttgggc taattttaat gttaacacac ctacctcaga
     4921 tctgatacaa catctacttt atgaaaaaag agaaaaaaca cttacagtag atttatctaa
     4981 gcgagtactt gcattagata aattaatagc agcaagttgc tcagcgtatt ctgcaaggaa
     5041 tcatacattg ctaaaaatga ttgaagcatt ttacccaaat gaatataaac aggttgtcaa
     5101 taag
//
LOCUS       DAOQQN010000236         5059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7554_length_5059_cov_5.491807, whole genome shotgun sequence.
ACCESSION   DAOQQN010000236 DAOQQN010000000
VERSION     DAOQQN010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7554_length_5059_cov_5.491807"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="PLG24_09125"
     CDS             complement(<1..978)
                     /locus_tag="PLG24_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25095.1"
                     /translation="MFLNICIILQSLFFPWLNNEHYQTPTLEKLNTRINTDAYDETNP
                     VVSKDGLTIYFTRVGHPDFNRTLMDDNTDLSATLSFADYQQELSSIYQTLSGRPVRDI
                     SQNSFNQDIWIATWAGGDFNNIEHPDYPLNSALPNSICSLTPDPDKFIIVNEFYKDGS
                     MYRGFSTIQDLGNGNWSFPKPLFIYDYFTQGNEVNLTMDEDGRIIIMSLKRPSGKGDN
                     DLYVSMKVADNLYSTPLPLNGVINTAYRESTPFISRDGQFLYFSSNRPGGYAGNDIYV
                     SRRLDETWQNWSEPALLPKPINSESDDSQPFVNEASGYIYFTSRRDGTSD"
     gene            1148..1303
                     /locus_tag="PLG24_09130"
     CDS             1148..1303
                     /locus_tag="PLG24_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25096.1"
                     /translation="MNIVKQQMLMFELAPKGNPEILFDMSDKKDLNVGFKLTKCKRFE
                     VANQVAE"
     gene            1349..2437
                     /gene="lpxK"
                     /locus_tag="PLG24_09135"
     CDS             1349..2437
                     /gene="lpxK"
                     /locus_tag="PLG24_09135"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HMZ25097.1"
                     /translation="MAFKVKRKNPLLSITFLLLPFAILFGIIVYLRNLLYDLKVIRPV
                     RFSVPTIGVGNLAVGGTGKSPHIEYLVKTLTPYIHVASLSRGYGRKTTGTIEVMNNAT
                     SEQVGDEPLVLKMKFPDISVFVGSNRAFAIPELMGRKPQTQVVLLDDIFQHRSVIPHE
                     NILLTEYSLPYFGDFLLPVGALREMPMGAKRADTIIVTKCPPEITLEQEEYFRKKIKP
                     GPNQQVYFSTFSYGDPYRLFQPTQRIKLQESMEIILFSAIAGTDYLMEYLKTQTKMIH
                     SIEFQDHHFFTGDEILQLKNTYTVLNAPEKIILTTEKDAVRLAMHREQIIAQQLPIFI
                     LPVEVKFIGSNAHLFDNYIKQRLLDFRI"
     gene            complement(2451..3872)
                     /gene="rlmD"
                     /locus_tag="PLG24_09140"
     CDS             complement(2451..3872)
                     /gene="rlmD"
                     /locus_tag="PLG24_09140"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HMZ25098.1"
                     /translation="MGRKRKGFKSIIFENREITGIAEESRSVCRTDDGIVCFVEGAVP
                     GDVVDVLVTQKKKGFYFGAVHNLVEASPDRIKAPCKHFGICGGCKWQHFHYAGQLREK
                     ENKVLQAMLRIGKTEPQEFQPILGCGEEFFYRNKLEFTFSNKRWLEPEEMSSEGDILK
                     EPGLGFHRAGAFDKVVDIEECMLQPDPSNAIRNFVKAFAIRMNWTFFNVRQREGFLRN
                     LMVKTSSIGEVMVLLSFHYKDDEAIGVLMEAIMTNFPEITTLVYVINEKANDTFLDLP
                     IEIYHGPGYIVEKLGEVKYRLGPKSFFQTNTNQAVRLYDLALEFAGLQPTDHVYDLYT
                     GLGSIALYASSRCKSIVGVENVAPAIEDARANADWNGISNATFVVGNVEDVVDTGFIT
                     KYGRPDVVITDPPRAGMHERVIDTLLQLECPKIVYISCNPSTQARDVAMLSTKYNLEK
                     LRAVDMFPQTNHIESVALLTLKK"
     gene            complement(3885..5039)
                     /locus_tag="PLG24_09145"
     CDS             complement(3885..5039)
                     /locus_tag="PLG24_09145"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HMZ25099.1"
                     /translation="MGLRFDAVQGVSIRPDVHFRKWWKESANNLSIKGGPVFGFSEKK
                     LRYAGEVTFARAGVKAYRIQLSAGNTADNYNTLEPISPFLNELTSLFFKRNYIKFYDN
                     TYIRLSGMHQVSPGFSLIGSVRAQHREGLLNHTDYSFRLKDENYQFNYPEEWSLENFY
                     KKSNAYTTSLTLRWQPGNKIIKLPERSFILGSSLPVVDLSLINAWAFSASDARYNRVN
                     LEVSNIKVALGALGTITSNIHGGLFYGKRPQYEMDYAHFPGNLVYVKNTNSYIDAFKR
                     FSYYKYSTPDKYLTMFAEWNLKGFLFKKIPLLDKTGFEEVFAINAMATPETGSRIEVS
                     AGIDRIGYSVFRGVRFDYIWIFENGKYLTDNFVIGLNLDAITSLFKGISQ"
BASE COUNT         1470 a         1158 c         1018 g         1413 t
ORIGIN      
        1 atcacttgtc ccatctcttc tggatgtaaa ataaatatat ccgctggctt cattgacaaa
       61 gggctgagaa tcatcggact ctgagttgat gggtttgggc aataaagccg gttcactcca
      121 attctgccat gtctcatcca atctcctgga gacatagata tcattgcctg cataccctcc
      181 gggtctgttg gacgaaaaat aaagaaactg cccgtcacgt gatataaatg gcgtggattc
      241 tcgatatgcc gtgtttatca caccatttaa aggaagaggg gtagaataca ggttgtctgc
      301 caccttcata cttacataca aatcattatc ccctttgccg ctcggtcttt tcaatgacat
      361 gataatgata cgtccgtctt cgtccatggt gagattcacc tcattgccct gagtaaaata
      421 atcatagata aacaatggtt tgggaaaaga ccaattgcca ttgcccaaat cctgtattgt
      481 tgaaaacccc cgatacatac tcccatcctt atagaattca ttgactataa tgaatttgtc
      541 cggatctggt gttagcgaac atatactatt gggtaatgca ctattaagag gataatccgg
      601 atgctcgatg ttattaaagt ctccgcctgc ccaggtggca atccagatat cttgattgaa
      661 gctgttctgt gaaatgtccc tgaccggtct cccggacaag gtttgatata tggatgatag
      721 ttcctgttga tagtcggcaa aagacaatgt cgcactcagg tcggtattat cgtccatcaa
      781 agtgcggttg aaatccggat gtcccactct tgtaaaataa atcgtaagcc cgtccttgct
      841 gactaccgga ttggtttcat cataagcatc cgtattaatc cgggtattta acttctcaag
      901 ggtcggcgtc tgataatgtt cattattcaa ccaagggaag aaaagacttt gcaatatgat
      961 acaaatattg agaaacatta cagattgatc agttaattaa atacatcaaa gtttgtcacc
     1021 cgggtaaatt cctccgcaaa gatataaaaa tttataatat gactggagaa gttaccggaa
     1081 aagattcgct taagagtgaa taaataagag gcgtgttagt tcgttccgtg caaatttaca
     1141 atattaaatg aacattgtaa aacaacaaat gttaatgttt gaattagctc ccaaaggcaa
     1201 tccggaaatt ttattcgata tgagtgataa aaaggatttg aatgtgggtt ttaaattaac
     1261 aaaatgcaaa aggtttgaag ttgcaaatca agtagccgaa taaccgaatt aactcttccg
     1321 tcccatatgc ttaaaataga ctttccctgt ggcctttaaa gtgaaacgca aaaatccgct
     1381 gctatccatt acatttctgt tgttaccctt tgccatactt ttcggcatca ttgtctattt
     1441 gaggaattta ctgtatgacc tgaaagtgat ccggcctgtc agattctctg taccgacgat
     1501 cggagtcggc aatctggctg tgggagggac cggtaaatca ccgcatatcg aatatctggt
     1561 aaaaacactg acaccataca tccatgttgc ttcgcttagt cggggatatg gacgaaagac
     1621 gaccggaact atcgaggtta tgaataatgc tacttccgaa caggtgggtg atgagccttt
     1681 ggtgttaaaa atgaagtttc ccgatatcag tgtatttgta ggctccaacc gggcatttgc
     1741 cattccggaa ctcatggggc ggaaaccaca gacgcaggtt gtccttctgg atgatatctt
     1801 ccagcatcgt tcagtgatac ctcatgagaa tatattgctt acagaataca gcctccccta
     1861 ctttggcgac ttccttctac cggtcggtgc actcagggaa atgcccatgg gtgcaaagcg
     1921 ggccgataca atcatcgtga ccaaatgccc tcctgaaatc actttggaac aagaagaata
     1981 tttccgaaaa aaaatcaagc ccgggccaaa tcagcaggta tatttcagca cattttcgta
     2041 tggagacccc taccgacttt tccagcctac acaaaggatc aagcttcagg aaagcatgga
     2101 aataatctta ttttcagcaa tcgcagggac ggattatctg atggaatacc taaaaactca
     2161 gaccaagatg atccactcca tcgaatttca ggatcatcac ttttttacag gagatgagat
     2221 cctacagcta aaaaatacct atacagttct gaatgctcct gaaaaaatca tcctgacaac
     2281 ggaaaaggat gctgtacgcc ttgccatgca ccgggaacag ataatagcac aacaattgcc
     2341 aatattcatt ttgcctgtag aagtaaaatt catcggtagc aatgcgcacc tgtttgataa
     2401 ctacatcaag cagaggctac ttgatttcag gatttagaaa gtgattccgt ttatttcttc
     2461 agggtcagca aggcaacaga ctctatatga ttggtctgag gaaacatgtc tactgcccgc
     2521 aacttttcaa gattgtattt agtgctcagc atggcgacat cacgtgcctg agtggatgga
     2581 ttgcagctga tgtatactat tttagggcat tccaattgca agagggtatc tattaccctt
     2641 tcatgcatac cggctctggg agggtcggta atcacgacat ccggtcttcc atattttgtt
     2701 ataaatccgg tatccaccac atcctcaaca ttaccaacca caaaggtggc attggagata
     2761 ccattccaat ctgcattggc cctggcatct tctatggcag gggcgacatt ctcgacacct
     2821 actatacttt tgcaacgtga cgaagcatac agggcgatac tgcccaaacc tgtatagagg
     2881 tcataaacat gatcggtggg ctgcagcccg gcaaactcaa gggccagatc gtataacctt
     2941 acagcctggt tagtattcgt ctgaaaaaat gacttagggc ctagccgata tttgacttca
     3001 cccagcttct ccacgatata acccgggccg tggtaaatct caataggaag atccagaaaa
     3061 gtatcattcg ccttttcatt gatgacatat accagcgttg taatttcggg aaaattagtc
     3121 atgatagcct ccataagcac cccaatcgct tcatcatcct tgtaatggaa agaaagtaac
     3181 accatcactt cgccgatgga agaggtttta accatcaggt tacgcaggaa gccctctctt
     3241 tgtctgacgt tgaaaaaagt ccagttcatc cggatggcaa atgctttgac aaaattgcga
     3301 atggcattgg atggatcggg ttgcaacata cattcttcga tatcgacaac cttatcaaag
     3361 gctcctgccc ggtgaaaacc aaggccaggc tcctttaaga tatcaccctc actactcatt
     3421 tcctccggct ctaaccagcg tttattagag aaggtaaatt ccagtttgtt acgatagaaa
     3481 aactcttctc cgcaacccag tatgggctgg aactcctgag gctctgtctt accgatacgc
     3541 aacattgcct gcaaaacctt attttccttt tcacgtaatt gtcccgcata atgaaagtgc
     3601 tgccatttgc agcccccgca aattccaaag tgtttgcagg gtgcctttat tctatccggt
     3661 gatgcttcaa ccaaattatg gactgctccg aaataaaagc ctttcttttt ctgtgtaacc
     3721 aggacatcca ctacatcccc cggaactgcc ccttcaacaa agcaaacgat tccgtcatcg
     3781 gtcctgcata ccgatctgct ttcttctgca atacccgtaa tctcccggtt ttcgaaaata
     3841 attgatttaa atcctttacg ttttctaccc ataacagaga ttacttattg gctgattcct
     3901 ttaaacaaac ttgtaatggc atccagattc aatccaatta caaagttgtc ggtcaggtac
     3961 ttgccatttt caaaaatcca gatataatca aatctaacgc cacggaatac ggaatatcca
     4021 atcctgtcaa ttcctgcact cacctcgatt ctggatcctg tttccggagt cgccatcgca
     4081 ttgattgcga aaacctcctc aaaaccggtt ttatccaaca atggaatttt cttaaaaaga
     4141 aagcccttca gattccattc cgcaaacatg gtaagatact tatcaggcgt actgtactta
     4201 taataggaaa accgcttaaa agcatcgata taagaattag tattcttaac atacaccagg
     4261 ttgccgggga aatgagcata atccatttca tattgcgggc gtttgccata gaaaaggcct
     4321 ccatgtatgt ttgatgttat tgtgcccagg gcacccaggg caactttgat attacttact
     4381 tccagattga cccgattgta tctggcatca cttgctgaaa aagcccaggc attgatcagg
     4441 gacaaatcta caaccggcag ggaagaacca aggatgaagg acctttcggg cagcttgatg
     4501 atcttgttgc cgggctgcca tcttaatgtc agggatgtgg tatatgcatt actcttctta
     4561 tagaaatttt caaggctcca ctcttccggg taattaaact gataattttc atccttgagt
     4621 ctgaagctgt aatctgtatg gttgagcagc ccttcccggt gctgggctct aacagacccg
     4681 attaaggaaa agccggggct gacctgatgc attccggaaa gcctaatgta ggtattgtcg
     4741 taaaatttta tgtaattcct tttaaagaac agactggtca attcattcaa aaaaggtgag
     4801 atcggttcca gtgtattgta attatctgct gtgttcccgg cagacaattg aattctatat
     4861 gccttgaccc cagcacgtgc aaaggtaacc tccccggcat accgaagttt cttttctgaa
     4921 aatccgaaaa ccggaccacc cttgatggac aggttattgg cagactcttt ccaccacttg
     4981 cgaaaatgaa catccggcct aatggataca ccttgaactg catcaaaacg gaggcccaac
     5041 tatcatctgc ccaatcatc
//
LOCUS       DAOQQN010000237         5030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7612_length_5030_cov_4.502513, whole genome shotgun sequence.
ACCESSION   DAOQQN010000237 DAOQQN010000000
VERSION     DAOQQN010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7612_length_5030_cov_4.502513"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1685
                     /locus_tag="PLG24_09150"
     CDS             <1..1685
                     /locus_tag="PLG24_09150"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HMZ25100.1"
                     /translation="NNLDYKVAKYPHELITYGGNGAVFQNWAQYLLTMKYLSEMTDEQ
                     TLALYSGHPMGLFPSHKEAPRVVVTNGMMIPNHSTKNDWNKYNALGVTQYGQMTAGSF
                     MYIGPQGIVHGTTITLLNAGRLMNLGDTLRGKVFVTSGLGGMSGAQALAGIITHAVTV
                     LAETDGDAIRQRTDDGYILKENIYADLEACVARMQSARSSEEVITLVYHGNVVDLWEY
                     FVIHNIDIELGSDQTSLHNIDDLGYTPVGYTFEEAKALMLRDHKKFREAVSASLIRHV
                     AAINALHDRGMYFWDYGNAFLKEAGVAGADVFDHDKKSGYRYPSYVEDIMGPLCFDFG
                     FGPFRWVCLSGLQEDLDMSDQIAGDVITSLMKTSAKDIMPQLQDNLQWIRNAKEQIPV
                     VGSKSRILYADAEGRIAIARAFNDAIRSGVLKGPVVLGRDHHDVSGTDSPYRETSNIY
                     DGSSFTADMAVHNVIGDSFRGATWVSLHNGGGVGWGEVINGGFGMVLDGSPEAERRLT
                     MMLHWDVYNGIARRSWARNPHAMSTIQRARDLYPHMVITEVNLVDSKVLDNL"
     gene            2043..3077
                     /locus_tag="PLG24_09155"
     CDS             2043..3077
                     /locus_tag="PLG24_09155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HMZ25101.1"
                     /translation="MIRFNIYPFLSILLMFLMSCCNNQSGVNQLEQYFAYGDSIESAG
                     VKMIPIHTPYGDFRVWTKRFGTNPDVKVLLLHGGPAMTHEYMECFETFFQRQGFELYE
                     YDQLGSFYSDQPENDSLWTIQRFVDEVDQVRQAIGADSNNFYLIGNSWGGLLAMEYAL
                     KHQDHLKGMVVSNMMASIPEYIQYFNNTLAPMMDPGALAEIKALEASKDYTNPRYEEL
                     LQEHYYNLHLCRLPEWPEPVTRSFSHGNNHIYVLMQGPSEMGIPPEARLYGWDIKSRL
                     HEITVPTLMVGARYDTMDPGAMEEQSKLVQRGRYLYCPNGSHLAMWDDQKVFMDGVIR
                     FIKDVDEGKM"
     gene            3132..4334
                     /locus_tag="PLG24_09160"
     CDS             3132..4334
                     /locus_tag="PLG24_09160"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HMZ25102.1"
                     /translation="MAYSESLSQSAFYRYLIPAFLILLSFTGCSQNSVSGHIVLSDVD
                     FKPVVYLLEPTGFSSLVSSYECKVVDSAEINEAGDFRFDKFSAPEKEGIFLLAIQKKG
                     EKYLNRLENNHPDSGNYIPFLLKKGYPVEIRSESGAFLRNGQIASTLEGNRDILVLIA
                     NRSRLFKQFLSDQAEVNEENLLENEKSLYNFQQQLVESVMGSQDLYVLALALQWAGIK
                     GDYERIPELVKATCKGMSAIEPEHVWTQQVCKKSGGLPLTPGDAFPDFMMPMTDGDTI
                     SLRSLLGSQLTIIDIWASWCAPCRKENRNTLVPLWDNFHDSGLQIIGYALESERVVWL
                     NAIKRDGADRWPHASHMEGDTSPFLDLLKITTIPANYILDRNGVILAKNLHGKELEEW
                     VTGKLGKE"
     gene            complement(4670..>5030)
                     /locus_tag="PLG24_09165"
     CDS             complement(4670..>5030)
                     /locus_tag="PLG24_09165"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HMZ25103.1"
                     /translation="LDADKIPAEIKTYVEKHFPGKNILQCIKDVDGLEVSYDVVVEGG
                     YKLEFNRDKKIDSVKGVSKLPDSVVPEKILAYVASNYPGNYIIEWDADKTTQEVKLDN
                     RLELVFNSAGDFLRIDD"
BASE COUNT         1348 a         1021 c         1237 g         1424 t
ORIGIN      
        1 agaataatct ggattataag gtggccaagt atcctcatga gttgattact tatggcggga
       61 atggagcagt cttccagaac tgggcccagt acctgcttac tatgaagtat ctgtctgaaa
      121 tgacagacga acagaccctg gccctgtatt ccggccaccc gatgggtctg ttccccagtc
      181 acaaagaagc gccaagagtc gtcgtaacca atggtatgat gatccctaac cattcgacta
      241 aaaatgactg gaataagtat aacgcgctcg gagtgactca atatgggcag atgacggccg
      301 gttcctttat gtatattggt ccgcagggta ttgtccatgg gacgactatt accttgctca
      361 atgcaggaag gttgatgaat ctcggagata cgctgcgtgg aaaagtcttt gtgacctcag
      421 gcctgggagg catgtcaggc gctcaggctc ttgccggcat cattacccat gcagtaactg
      481 tgttggctga aacagatggt gatgctatcc gccaacggac tgacgatggt tatattctga
      541 aagaaaatat ctatgccgat ttggaggctt gtgttgcaag aatgcagtcg gcacggtcat
      601 ccgaggaggt aattactctg gtttaccatg gaaatgtggt tgatctgtgg gagtatttcg
      661 tgattcacaa catcgatatc gaactggggt ctgatcagac ttcgttgcac aatattgatg
      721 acctgggata tacacctgtc ggatatacct ttgaagaggc caaggccttg atgctccgtg
      781 atcataaaaa atttcgtgag gcggtttcag cttcgttgat tcgacatgtt gccgccataa
      841 atgccctcca tgacagggga atgtattttt gggattatgg caatgccttc ctgaaggaag
      901 ccggtgtcgc aggtgcagat gtatttgatc atgacaagaa aagtggatat cggtacccat
      961 cctatgtaga agatatcatg ggtcctcttt gttttgactt cggattcggc ccgtttaggt
     1021 gggtttgctt aagtgggctg caggaagacc ttgatatgag cgatcaaatc gcaggtgatg
     1081 ttattacttc gttgatgaag acatcggcaa aggatatcat gccacaattg caggacaatc
     1141 tacaatggat tcgcaatgcc aaagagcaga ttcctgtcgt tggctccaaa tcccggattc
     1201 tttacgctga tgcggaaggc aggattgcca ttgcccgcgc atttaatgat gcgatacgtt
     1261 ccggtgtgct gaaaggccct gtggttcttg gccgtgacca ccatgatgtg tcagggacag
     1321 attcacccta tcgggagacc tccaatatct atgacggatc atcatttacg gcagatatgg
     1381 cagtgcataa tgttatcggg gacagttttc ggggagcaac ctgggtgtcg ctgcataatg
     1441 gaggtggggt aggttggggc gaagtcatca acggcggttt tggcatggtg ctggatggaa
     1501 gccctgaagc agaacgacgt ctgaccatga tgttacattg ggacgtgtac aatggcattg
     1561 caaggcgttc ctgggcaagg aatcctcacg ctatgagtac aattcagcgt gccagagacc
     1621 tatatcctca catggtcatc accgaagtta atcttgtgga tagcaaggtt ttggacaact
     1681 tgtaataaca atccatggtg ccggaaccat tttggtgcac gaaaaatcgc ctgatgtttg
     1741 gatcagagac ccttcaattc gaatggaaag gatggtaaat atgtgtaatt tgccgggagt
     1801 catcggagtc ttgtttactc tttgtccagt cacattcgga ggtggcgcat tttcaggtat
     1861 tactttcatt attctgatct tgtcttttaa aaggaagtgt cacttctccg gttattttga
     1921 gggatttctt gccgctttcg accaccatag tcatggctca gaatgtatcg aaactgtccg
     1981 gatacttagg gcgacagaac caaaaagaaa ccattgaata actgtgctca actaaaaata
     2041 ttatgataag atttaatata taccctttcc tgagcatcct gttgatgttt ttaatgtcat
     2101 gttgcaataa tcaatcaggt gtcaatcagc ttgagcagta ttttgcttat ggtgattcta
     2161 tagaatcggc aggcgtgaag atgattccca tccatacgcc ctatggtgat tttagggtgt
     2221 ggaccaagcg attcggcacc aatcctgatg taaaggttct cttgctgcat ggtgggccgg
     2281 cgatgacgca tgaatatatg gaatgcttcg aaaccttctt tcaacggcaa ggcttcgaac
     2341 tgtatgaata tgaccagttg ggctccttct acagcgatca acctgagaat gacagtttat
     2401 ggactattca acgatttgta gatgaggtgg atcaggtcag acaggctatt ggtgcggaca
     2461 gcaacaactt ttatctgatt ggcaattcct ggggcggact tctggctatg gaatacgcac
     2521 tcaagcatca ggatcatcta aaaggaatgg tggtgtctaa tatgatggcc agcattcctg
     2581 aatatattca atattttaat aataccctgg ctcccatgat ggatcccgga gcactggctg
     2641 aaatcaaagc actggaggct tcaaaggatt acaccaatcc gcgatatgag gaattattgc
     2701 aagaacatta ttacaacctg cacctttgcc gcctcccaga atggcctgaa cctgtgactc
     2761 gttccttcag tcatggtaat aatcatatat atgtattgat gcagggcccg agtgaaatgg
     2821 gcatcccgcc ggaagccaga ttatacggtt gggatatcaa gtcgcggctg catgaaatta
     2881 ccgttccaac cttgatggtg ggagcacggt atgataccat ggatcccggg gcaatggaag
     2941 agcaaagcaa attggtccaa agggggcgat acctgtattg ccccaatggc agccacctgg
     3001 ccatgtggga tgaccagaag gtatttatgg acggcgtgat cagatttatt aaagatgtgg
     3061 atgaagggaa aatgtaatat actaaccgaa tccatcgtaa ggacatagat tgttggctaa
     3121 aatttgatga gatggcttat tctgaatcat tatcacaaag tgctttctac aggtatttga
     3181 ttcctgcatt ccttatctta ctgtcattta ccggatgcag tcagaatagt gtttccggac
     3241 atattgtcct gagtgatgtt gatttcaagc cggttgttta tttacttgaa cctacgggat
     3301 tcagttcttt ggtgtcttcc tatgaatgta aggttgtcga ttctgctgag ataaatgaag
     3361 caggtgattt ccggttcgac aaattttcag cgcctgaaaa agaggggatt ttcctgcttg
     3421 ccatccagaa aaaaggcgag aaatatctca accgactgga gaataatcat ccggactccg
     3481 ggaattatat tccattttta ttgaagaagg gttacccggt agagattcgg tccgaatccg
     3541 gggcttttct tcgaaatggt cagatagcga gtactttgga aggcaatagg gacattcttg
     3601 tactgatagc gaatagaagt aggcttttta aacaatttct ttccgatcag gctgaggtaa
     3661 acgaggagaa tcttttggaa aatgaaaagt ctctatacaa ctttcagcaa caattggtgg
     3721 aatcggtgat gggtagtcag gatttgtacg tacttgcatt agccttgcaa tgggcgggca
     3781 tcaagggtga ttatgaaaga attcccgaat tggtaaaagc cacttgcaag ggcatgtcag
     3841 caatagaacc ggagcatgtg tggacccaac aggtgtgtaa aaagtccggc ggattgccct
     3901 tgactccggg ggatgcattt cctgatttta tgatgccaat gacagatgga gacaccattt
     3961 ctctgcgttc gctattaggg tctcagttga ccataatcga tatttgggct tcctggtgtg
     4021 caccttgcag aaaagaaaac agaaatacac ttgttccact ctgggataac tttcatgact
     4081 caggccttca gattataggc tatgcactgg aatccgagcg ggtagtgtgg ttgaatgcca
     4141 tcaagcggga tggtgcagat cgatggcctc acgcctctca tatggaggga gatacatctc
     4201 catttctgga cttgttaaag atcacaacaa ttccggccaa ttatatcctg gacagaaatg
     4261 gtgtcatcct ggccaaaaat cttcatggca aggaattgga ggaatgggtg accgggaaat
     4321 taggaaaaga ataatccaac ctggagcctg ttttttcatt tgaaaaaata aatccggatt
     4381 gacttaggta cttgaattct gccgaagcaa gaggaaaata ctgacagggc tcatcattat
     4441 ggattttgtc ttatgatatc ctgaatattt tacccggccg gaggacctgt ttcttccgtt
     4501 tgaattcagc ataagcaact tatgccaatc cggaatttta ttagattgaa attgaaaaat
     4561 tcacgcctac ttgtattcgt tatgcgaaac cgatgtagga tttgagcaat aaaatcaccg
     4621 aattatgccg aaatgccaac ttgccggttc aatgtaatac ttttcttgat tagtcatcga
     4681 tgcgaagaaa gtctccggca ctgttgaata ccagttcgag cctgttgtcc agcttgactt
     4741 cctgagtcgt cttatcggca tcccactcga tgatataatt gcccgggtag ttgcttgcca
     4801 cgtacgctaa aattttctca ggtacaacgg aatcaggcaa tttagagaca cctttgacgc
     4861 tgtcgatctt tttgtcccgg ttaaattcaa gtttatagcc tccttcaaca accacatcat
     4921 agcttacttc caacccatca acatctttga tacattgtag aatatttttc ccggggaaat
     4981 gtttttcgac atatgtttta atttctgccg gtattttatc tgcatcgagc
//
LOCUS       DAOQQN010000238         5021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7632_length_5021_cov_5.656665, whole genome shotgun sequence.
ACCESSION   DAOQQN010000238 DAOQQN010000000
VERSION     DAOQQN010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7632_length_5021_cov_5.656665"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..848)
                     /locus_tag="PLG24_09170"
     CDS             complement(78..848)
                     /locus_tag="PLG24_09170"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ25104.1"
                     /translation="MVKTILLTGATSGIGKATARELSAMGRYRLILTGRRKERLEALK
                     EELIRSDKTEVHVFAFDIRDLNACKVFWDGLPDGLKQIDILINNAGLAKGLSEIHEGS
                     FDHWEQMIDTNIKGLLHMTRLISPGMVERRQGHIINLGSTAGKETYPNGNVYNATKFA
                     VEALTRAMRMDLYKYGIRVSSVSPGAVEETEFALVRFDGDAQRARIYDDYTPLKAKDV
                     AEIIAFILSRPPHVNIQDVLVTSTQQASSMLFDKTGRK"
     gene            complement(842..2065)
                     /locus_tag="PLG24_09175"
     CDS             complement(842..2065)
                     /locus_tag="PLG24_09175"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMZ25105.1"
                     /translation="MPGIWRGELYLDESNPLSRNISKVGNDQDISFKNAFTEGVLPFN
                     FEVTYEGDSMFVDFINGDNRVRAEETLWGFNRRSGKDTIRINFVAYDTYLRAEVEDGI
                     MTGAWYNPRKNWRVPFRGLAGKDYRFTMVNEKPSFDISGKWDVIFHFENRNDTTIGQF
                     TQDNNSLKGTFASPTGDYGFLEGTVQGDRMFLSGFAGGAAYLVSGKITEKGRMKGTFM
                     SGLTSRATWEAVFNPDAILPDASILTRASGNQAVNFTFPDEQGNLVSLSDPTFNGKAK
                     VLTITGTWCPNCKDEARFLTEYLNKRQNDRLSVIALSFEAFDDTAKVRSLLSNYKKVL
                     DLPYTVLWAGKPGPEASSRVPFISGIKAYPTMIFLDRNNHVVSVHTGFSGPATDEYEA
                     FKKDFDLQVEKLTKW"
     gene            complement(2212..2739)
                     /locus_tag="PLG24_09180"
     CDS             complement(2212..2739)
                     /locus_tag="PLG24_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25106.1"
                     /translation="MELYGVGSRLSHPSYGDGVVIKLNPENYEICFMTYGIKQISKDY
                     DRFSEIERIEPSEEVSFSDAEKSLARILQQWMGITELVPLGDKWKGGIMELKPSNPSL
                     KSKEMPIESFFHKIVMLRDRLRVMEQRINSHDAFSDEDKVNMQQYITRIYGSLTSFNV
                     LFKYPEHQFVGEKSS"
     gene            complement(2806..3384)
                     /locus_tag="PLG24_09185"
     CDS             complement(2806..3384)
                     /locus_tag="PLG24_09185"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HMZ25107.1"
                     /translation="MDFLKKHTLFLASGSPRRADLMRQAGFLFTVINNDVDESYPENL
                     DVTKVAEYLSLVKAEAAKEFIVDDNSVVIAADSVVIIDGEIIGKPVDRADAQRILRKL
                     SGRAHVVITGVTLLGKLKKVTFSESATVHFYQLTDDEIDFYLSNFSPMDKAGAYGIQD
                     WIGVCRVRSITGNFSNIMGLPVSRLYNELKDF"
     gene            complement(3447..4376)
                     /locus_tag="PLG24_09190"
     CDS             complement(3447..4376)
                     /locus_tag="PLG24_09190"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HMZ25108.1"
                     /translation="MTSLRDILKLIRPINALIVFATLMVVRLILFQYYNPFKIQPSID
                     AADYFLMMVTGMLILGAGNVINDIIDVDIDRLNKPRKALIPRRVSIRLAWVVYFSLNI
                     LALIFSVYLILKYQVGWYIFMPFCAILLLYVYSRWLKKKFFIGNLVIALLCGVLGLVG
                     FWVEMDNIHTIKELSDKDYTMILYSAGSIFIFSFLLVLARELIKDCEDMVGDRSAGAT
                     TIPIKLGLVRSDRLIMFLWVLYGVVFLALSIARYKLYESTPMMIAIIIYLIIYVLSIR
                     WIMVRFADKNLYSRLSTLVKIFLILGLIFILIK"
     gene            complement(4440..4967)
                     /locus_tag="PLG24_09195"
     CDS             complement(4440..4967)
                     /locus_tag="PLG24_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HMZ25109.1"
                     /translation="MDLFDKMRAVKAFVFDVDGVMTDSRVHVWENGDQTRTMNTRDGF
                     ALKRAILKGYRVAVITGGKSKGVTSRLNGLGVLDVYSGIADKLIALEDFRKRYDVSYD
                     QIMYMGDDLIDASVMKRVGLPAAPRDAVPAILNISSFVSSLDGGDGCVREIIEKIMIL
                     QGTWDWEVGNEQPGI"
BASE COUNT         1526 a         1168 c          877 g         1450 t
ORIGIN      
        1 attgaagacc ggatccattg acagagactg gctcgaaaag gcaggcccgt accttattgc
       61 cggtaaataa ttcctgctta ctttcggcct gtcttgtcaa aaagcattga actggcttgt
      121 tgggtagagg taacaagtac gtcctgtata ttgacatgtg gtggccggga gagaatgaat
      181 gcaatgatct ctgcaacatc cttagccttg agtggtgtat aatcgtcata aattcttgct
      241 ctctgagcat ctccatcaaa gcgcacaagg gcgaattcag tctcttccac agcaccggga
      301 ctgactgagg aaacacggat tccgtatttg tacaaatcca ttcgcattgc tcgggttagt
      361 gcttctacag cgaatttggt agcgttatat acattaccat tgggataggt ctccttgcct
      421 gctgtagagc cgagattgat gatgtggcct tgcctacgct cgaccattcc gggagaaatg
      481 agtctggtca tatggaggag acctttaata ttggtatcga tcatttgttc ccaatggtca
      541 aacgatcctt cgtgaatttc actcaaacct ttagccagac ctgcattgtt gataaggata
      601 tcgatctgct tcaaaccatc cggaaggcca tcccaaaaaa ctttgcatgc gtttaaatcc
      661 cggatatcaa aagcaaaaac atgaacttcg gttttgtccg accgaatgag ctcttcctta
      721 agcgcttcca acctctcttt ccttcttccg gttaatatca acctatagcg tcccatggca
      781 cttaattcac gtgccgtggc cttgccgatg cctgaagtcg ctccggtcag gagtattgtc
      841 ttcaccattt tgttaatttt tctacctgaa gatcaaaatc ttttttaaaa gcttcgtatt
      901 catctgttgc gggaccgctg aatccggtat gcacactgac aacatgattg ttcctgtcca
      961 gaaaaatcat cgtcggataa gcctttatcc cactgataaa tggcactcgt gaagaggctt
     1021 caggccccgg ctttcccgcc cacaaaactg tatagggcaa atcaagtacc tttttataat
     1081 tagacaacaa cgatctgacc tttgcggtat catcaaatgc ttcaaatgac aaagctataa
     1141 ctgaaagcct atcgttttga cgcttattca aatattcagt taaaaatctg gcttcatcct
     1201 tacaattggg gcaccaggtt ccggttattg taagaacttt tgcctttcca ttgaaagttg
     1261 gatcagaaag actcaccaaa ttgccttgct catcaggaaa agtaaagttt acagcctgat
     1321 ttccggaggc cctggtaagg atggatgcat caggcaagat tgcatcagga ttaaaaacag
     1381 cttcccatgt agctctgctc gtcaaccctg acataaatgt acccttcatc ctgccttttt
     1441 ctgttatttt accgctgacc aaataagctg ctccaccggc gaatccgctc aaaaacattc
     1501 tatccccttg tacggtacct tccaggaagc catagtctcc ggtaggagaa gcaaatgttc
     1561 ctttaaggct gttattatct tgtgtaaatt gaccgatggt agtatcattc ctgttttcaa
     1621 agtgaaaaat aacatcccac ttgcccgaaa tatcaaatga aggcttttcg tttaccattg
     1681 taaaccgata atcttttccg gcaagtcctc tgaacgggac tctccagttt ttccgggggt
     1741 tataccatgc gcctgtcatg atgccatcct ccacttcagc ccttaaataa gtatcataag
     1801 caacaaaatt gatccgtatt gtgtcctttc cacttcttct gttgaaaccc cataatgtct
     1861 cttctgccct cacccgatta tctccattga taaaatcgac aaacatgctg tcaccctcgt
     1921 aggtgacctc gaaattaaat ggcagtactc cctctgtaaa ggcattctta aaagagatat
     1981 cctgatcatt accaactttg gaaatatttc ttgagagggg attgctttca tccagatata
     2041 attcaccccg ccaaataccg ggcattagtt tgtcaaattt ctgattggtt accaaacagg
     2101 aattgaaaat ataaataact aacaaaggaa gtaagacaac tttccaccac ttggataaca
     2161 tataattctt ttacaagtaa accaacaaaa tttaaatcag tttatcccga atcaactgct
     2221 tttttcacca acgaactgat gttccggata tttaaacaag acattgaagg aagtcaatga
     2281 cccatagatc ctggtaatat attgttgcat attgacttta tcttcatcac taaaggcatc
     2341 atgactgttg atacgctgct ccataacacg tagacggtcc ctaagcataa ctattttgtg
     2401 aaaaaaagac tctatcggca tttcctttga cttcaaagaa ggattcgaag gcttcagctc
     2461 catgattcct cccttccatt tatctcctaa aggcaccaat tcagttatgc ccatccattg
     2521 ttgaaggatt cgtgccaggg atttttcggc atctgaaaaa ctaacctctt cacttggttc
     2581 gattctttca atctcagaaa accggtcata atccttactg atttgcttga tgccatatgt
     2641 catgaaacaa atttcataat tttcaggatt cagtttaata actactccat caccatagga
     2701 cggatgactt agtcgacttc caactccata taactccata gatttcaaat tttaagccgt
     2761 aaaatagcaa tatctttaca aacaggtgta aaaattggtg atagatcaaa aatccttcaa
     2821 ttcattatac agcctactca ccggaagacc cataatattg ctgaaattgc ctgtaataga
     2881 tctaaccctg caaactccaa tccaatcctg gataccatag gcacctgcct tatccatcgg
     2941 agaaaaattc gataaataaa aatcaatctc atcgtctgtc agttgataaa aatggactgt
     3001 agccgattcg ctgaaagtca ccttctttaa tttgccgagt aaagtaactc cggtaatcac
     3061 gacatgtgcc cgaccggaca attttcttaa tatcctttgt gcatcagccc gatcaacagg
     3121 cttacctatt atttccccat cgataataac gacactgtcg gctgcaatta ccaccgaatt
     3181 gtcatcaaca atgaattctt ttgcagcctc tgcttttaca agggataaat attcagcaac
     3241 tttagtgaca tcaagattct cgggataaga ctcatcaaca tcattattaa tcacagtaaa
     3301 taagaaaccg gcctgccgca ttaaatcagc tctacgtggg ctcccggagg ccaggaaaag
     3361 tgtgtgtttc tttagaaaat ccatgatact tctaaaaaga gttgaaattt atatattatt
     3421 ttgtataata tttattttac aaataatcat tttattaata taaaaattag tcctaaaatc
     3481 aaaaatatct tgacaagcgt actcagtcgg gagtataaat tcttgtctgc aaatctaacc
     3541 atgatccatc tgatgcttaa aacataaatt atcaaataaa tgataatagc tatcatcatc
     3601 ggagtggatt catacagctt atatctggcg atggataaag ccagaaaaac gacaccatat
     3661 aaaacccaga gaaacatgat caaacgatca gagcgtacaa gaccaagttt aatcggtatt
     3721 gtagttgcgc cggcactccg atctccgacc atgtcttcac aatcctttat caattcgcgc
     3781 gcaagtacca gcaaaaaact gaaaataaag atacttccgg ctgaatataa aatcatggta
     3841 taatctttat ctgatagctc tttaatagtg tgaatattgt ccatctctac ccaaaatcca
     3901 accaaaccga gtactccaca gagaagagcg atcactaaat tcccaataaa aaacttcttc
     3961 tttagccaac gtgagtacac atacaacaac aagattgcac aaaatggcat gaaaatatac
     4021 cagccaacct gatatttcag gatcaaatag actgaaaaaa tcaatgcaag gatattcaat
     4081 gagaaatata ccacccatgc cagcctgatt gaaacacgtc tcgggataag tgctttccgg
     4141 ggcttattaa gtcggtcgat atcgacatcg ataatatcgt tgattacatt accggctccc
     4201 agaataagca taccggtgac catcatcagg aaatagtccg ctgcatctat ggaaggctga
     4261 attttaaaag gattataata ttggaaaaga atcaacctca ccaccatcag tgtagcgaat
     4321 acaatcaagg cattgatagg cctgatgagc tttaaaatat cccgtagaga agtcacagcc
     4381 taaaggtatg gattgtaggc taatccgcct taatttttga ttggtgtttg ataaataaat
     4441 tatatacctg gctgttcgtt gcctacctcc caatcccagg tcccttgtaa tatcataatt
     4501 ttctcaatga tctcccgtac gcatccatca cccccatcca atgatgaaac aaatgaagat
     4561 atattaagaa tggccggtac agcatcccgc ggggctgcag gcagtccgac gcgtttcatc
     4621 accgaagcat cgattagatc atcccccatg tacatgattt gatcataact tacgtcatac
     4681 cttttgcgaa aatcttccaa agcaatcaat ttatcagcaa ttcctgaata aacgtcaaga
     4741 acaccaagac cattgagcct ggatgtcaca cccttgcttt tacctccggt aatgacggcc
     4801 acacgatatc cttttaaaat ggcccttttt aatgcaaatc catctctggt gttcattgtt
     4861 cgtgtctgat cgccattctc ccaaacatgt actctggaat cagtcattac tccgtcaaca
     4921 tcaaagacga aggctttgac agctctcatt ttgtcgaaca aatccatagc ctattgatta
     4981 tctcgccact cgtacaccca gtgtgtctgg atctgctcca g
//
LOCUS       DAOQQN010000239         5004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7670_length_5004_cov_5.286725, whole genome shotgun sequence.
ACCESSION   DAOQQN010000239 DAOQQN010000000
VERSION     DAOQQN010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7670_length_5004_cov_5.286725"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLG24_09200"
     CDS             complement(<1..287)
                     /locus_tag="PLG24_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HMZ25110.1"
                     /translation="MPELLKDKFTSLNICVIGDLILDEYIIGKVTRVSPEAPIPVVDQ
                     QAREYKPGGAANVALNLINLGVNTYLAGVVGDDTHGLILKKLIEEVLPEKG"
     gene            complement(265..1464)
                     /locus_tag="PLG24_09205"
     CDS             complement(265..1464)
                     /locus_tag="PLG24_09205"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HMZ25111.1"
                     /translation="MFLCYSCINSKAAKIRIGRKQLIRYLGSTSSGLPTVWFHCSSLG
                     EWEQTIPVVERLKEKSNVQILVSFYSSSGFEYAKRRDLVDAMIYLPVDFYKAYAPVFN
                     TLNLVGISFTRYDIWPNLVRHAVDRNVPLFLIGAESASLRKYRRSNGFFAKMLRSFSF
                     ISCNTVSAATLLNNSGYRHVYTDGSSKLERSAQIAAQEYVNASLDSWRKDAFVIIGGS
                     IWPPEAIMMERLFIEGAIENMKMILVPHEPSHDVIGALQEKYKDTRVLFSQMDGNSTE
                     SKVIIMDKVGFLSKIYRFADVAIVGGGFGKGIHNIAEPAASGLAVISGPNNSGFSEAA
                     MLQDAGVYFPIETYKEFRELVIDFAENELKRKEIRTIANTFFNAQSEAAERISQHIVD
                     YAGITER"
     gene            complement(1518..2273)
                     /locus_tag="PLG24_09210"
     CDS             complement(1518..2273)
                     /locus_tag="PLG24_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25112.1"
                     /translation="MVFCKRLIYIIVLCHILVVSAISQEKTSKARSALLIHLDYGFNL
                     PAADLAKRFGSNFQVGGDVEYKFPVDIFIGVNYSYLWGKKVKENIGENLFNAKNEIVG
                     IDNHLANLVIRERGFSSSLNVGKLFRIFPNEAAALKVGIGGGFLQHKVRIVDDYGVVP
                     QITGDYLKGYDRLTNGFMTRQYIGYQYLSTNRKINFSVGFVFAQGFTRSARGFNYDTG
                     KKDTEKRFDLLNGLQVSWILPIYFNDYSEEIIY"
     gene            2394..3485
                     /locus_tag="PLG24_09215"
     CDS             2394..3485
                     /locus_tag="PLG24_09215"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HMZ25113.1"
                     /translation="MNDLGAQYLRYKDEIDRAIQNVIDSGQFIKGPIVNQFEADLSKY
                     LGGVEVITCGNGTDALQIALMALDLKEGDEVITTPFTFAATAEVIALLKLKPVFVDIK
                     PDTYCIDPTKIEEAITPATRCIIPVHLFGTIADMNAIMAIADLYGLYVIEDAAQSLGS
                     LYKMNDGNTVHSGTIGHIGTTSFFPSKNLGCFGDGGALFTKDRVLAEKIRMIANHGTK
                     VKYNHEIIGVNSRLDSIQAAILNVKLQYLDMDISARIKAAAIYDQLLHGHGEITSPIA
                     TENATHSYHQYTVRVKNRSKVRVRLGEAGISSMIYYPIPLHIQKAFAFAGGVIGRFPV
                     TECACDEVLSLPIYPDITKEKIEKVVAHL"
     gene            3482..4420
                     /locus_tag="PLG24_09220"
     CDS             3482..4420
                     /locus_tag="PLG24_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ25114.1"
                     /translation="MKKVLITGAAGFLGSHLCDRFIREGNHVIGMDNFITGNPDNIAH
                     LFPLKHFEFHHHDVSKFVHITGHLDYILHFASPASPIDYLKMPIQTLKVGSLGTHNLL
                     GLAKAKNARMLIASTSEVYGDPLVHPQREDYWGNVNPIGPRGVYDEAKRFQEAMTMAY
                     HNFHGLETRIVRIFNTYGPRMRVNDGRALPAFFSQAIKGQNLTVFGDGSQTRSFCYVD
                     DLVEGIYRLLLSDYHLPVNIGNPEEITLNQCADEVIEIVGNPEVKIEYHDLPKDDPKV
                     RRPDITLARQLLGWEPTVSRNEGLRATFEYFKKALS"
     gene            4508..>5004
                     /locus_tag="PLG24_09225"
     CDS             4508..>5004
                     /locus_tag="PLG24_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25115.1"
                     /translation="MNKIYILFLLLIIPALSYSQIDNKDMKMLMLQNSGKIDKIKADI
                     KAKGITEAELSSKLKQKGIDLQNLQPEQIPTLELMVDEAIREIENEKIKAKSEIETKP
                     STSDKKEKEVDLDNFEFSRNFTKSIEESFDLKGNKQKTARFVSDSLKAADTIPFAIFG
                     QQIFK"
BASE COUNT         1557 a         1103 c          994 g         1350 t
ORIGIN      
        1 tgaccttttt cgggcaacac ttcctcaatc aattttttta aaattaatcc atgtgtatca
       61 tcccccacaa cacctgccag atacgtattc acgccaaggt tgatcaaatt gagggcgacg
      121 ttggcagctc cgcccggctt gtactccctc gcctgctgat ccaccacagg aataggagcc
      181 tcaggcgaga ccctggttac cttaccgatg atgtattcgt ccaggatcaa atctcctatg
      241 acacaaatat ttaacgatgt aaacttatct ttcagtaatt ccggcataat ccacaatatg
      301 ttgacttatt cgctctgctg cctccgactg ggcattaaaa aatgtgttgg caatggtccg
      361 tatttctttt cttttcaatt cattctcagc aaaatcgatc actaactcac gaaattcttt
      421 ataggtttca attggaaaat acacacctgc gtcttgtagc atggcagctt ccgaaaaacc
      481 tgaattattc ggacctgaaa tcacggccaa tccagaagca gccggctcag ctatattatg
      541 tattcccttt ccgaatcctc ccccaacaat ggcaacatct gcaaatctat atatttttga
      601 taaaaaacct accttatcca taataatcac cttactctcc gtactgttcc cgtccatttg
      661 actgaaaagc acccgggtgt ctttatattt ctcttgaaga gccccgatca catcatggga
      721 aggctcatga ggcacaagaa tcatcttcat attttctata gcgccctcta taaaaagcct
      781 ttccatcatg atagcctccg gtggccatat actacctccg atgatcacga atgcatcctt
      841 cctccagcta tccaaggatg cattaacata ctcttgagca gcaatttgcg ccgatctttc
      901 cagtttggaa cttccatcag tatacacatg tctgtatccc gaattgttca atagtgttgc
      961 cgcacttacc gtattacagc ttataaacga gaatgaccgc aacattttcg cgaaaaaccc
     1021 atttgacctc ctgtatttcc tcaaagatgc actctcagca ccaatcaaaa acagaggcac
     1081 attcctgtcg actgcatgtc tgacaagatt aggccagata tcataacgcg taaaactgat
     1141 accgacaagg ttcaatgtat taaaaacggg tgcataagcc ttataaaaat caaccggcaa
     1201 ataaatcatt gcatccacca aatcgcgccg tttggcatat tcaaaaccgg atgaactgta
     1261 gaaggacacc agaatttgga cattggactt ctcctttaat ctttcaacca cagggatagt
     1321 ttgttcccac tcacccaggg aagagcaatg aaaccaaacg gtaggcaagc cggaagatgt
     1381 ggatcctaaa tatcggatga gctgctttct tccgattcta attttagcag ctttagaatt
     1441 gatgcatgaa tagcacaaaa acaacaggtg gtaaattcga ataccgaaat tatataatat
     1501 ataggccaca gattgggtca atagataatc tcttctgaat aatcgttgaa ataaataggc
     1561 agtatccagc taacttgaag gccattgagc aaatcaaatc tcttctcggt atctttcttg
     1621 ccggtgtcgt aattaaatcc gcgtgcactg cgggtaaatc cctgcgcaaa aacaaaacca
     1681 acggaaaagt tgatctttct attggtggat agatactgat atccaatata ctgccgggtc
     1741 ataaagccgt tggtaagcct gtcataacct ttcagatagt cgccagttat ctgtggcacg
     1801 actccataat catccacgat tctcacctta tgttgcaaaa agcctccacc aatgccaact
     1861 ttcagtgctg cggcttcatt tggaaaaatt ctgaataact ttccgacatt cagggaagaa
     1921 gaaaaacctc tttcccggat gacaagattt gcaagatggt tgtcaattcc gactatttca
     1981 ttcttggcgt taaacaagtt ttcaccaata ttctctttaa cttttttacc ccacaaatag
     2041 gaataattca ctccaatgaa gatatcaacc ggaaatttat attccacatc tccaccaacc
     2101 tgaaaattac tgccgaagcg tttggccaaa tctgcagcag gcaggttgaa gccataatcc
     2161 aggtgtatga gtaacgcact tcttgccttg ctcgtttttt cctgagaaat ggctgaaaca
     2221 accagtatat gacacaggac aatgatgtaa atcaatcttt tacaaaaaac cataatgcaa
     2281 agttagtaaa ttgaatcatc aaatatggca taattaatag gaactaatag cttacttttg
     2341 ccctgttttt cttttacacc aaaaacatac aacttttgga tcaaaaaatt ccgatgaacg
     2401 acttgggtgc ccaataccta agatacaagg acgagattga ccgggccatt caaaatgtca
     2461 tcgatagtgg acagtttatc aaagggccaa ttgtcaatca gtttgaagcg gatctttcta
     2521 aatatctggg aggtgttgaa gtaatcactt gcggtaatgg cacggacgca ttgcaaatag
     2581 ctttgatggc tctggatctg aaggaaggcg atgaagttat aactacgcct tttacctttg
     2641 ctgcgactgc agaagtgata gctcttttga aactgaaacc tgtatttgtg gatatcaagc
     2701 cggacaccta ttgtattgat ccaacaaaaa tcgaggaggc aataacaccg gccaccagat
     2761 gtatcattcc ggtccatctt ttcggaacaa ttgcagatat gaatgctatc atggccattg
     2821 ctgacttata tggcctttat gtgatagagg acgctgccca atctttggga tcattgtata
     2881 agatgaatga tgggaatact gttcactccg gaaccatcgg tcatataggg acaacaagct
     2941 tctttccctc aaaaaaccta ggttgttttg gtgatggtgg ggctctgttt accaaagata
     3001 gggtattggc cgaaaagata cgaatgattg ccaatcacgg tacaaaggtc aagtacaatc
     3061 atgaaatcat cggggtgaac agtagactgg actctattca agccgccata ctgaatgtta
     3121 aactgcaata ccttgacatg gatatcagtg caagaataaa agcagctgca atatatgatc
     3181 aattgcttca tgggcatgga gagattactt cgcctatagc aacggagaat gccactcaca
     3241 gttatcatca atacaccgtt cgcgtcaaga atagatccaa ggtaagggtc agattgggtg
     3301 aggcgggcat atcttccatg atctattacc cgataccctt gcatatccaa aaggcgtttg
     3361 cctttgcagg aggggtcatc ggccgatttc cagtgaccga atgtgcctgt gatgaagtat
     3421 tgagccttcc tatctacccc gatatcacca aggaaaaaat agaaaaagta gtagcacatc
     3481 tatgaaaaaa gtactgataa ccggagctgc cggatttttg ggttcccacc tctgtgacag
     3541 attcatcaga gaaggtaatc atgtcatcgg gatggacaac tttataacgg gcaatccgga
     3601 taacatcgcc catcttttcc cgttaaaaca ttttgaattc caccatcacg atgtctcaaa
     3661 attcgttcac ataacaggtc atctggatta tatccttcat tttgcatcac cggcttcacc
     3721 aattgactat ctgaagatgc ctattcagac actcaaagtg ggctccctcg gcacacacaa
     3781 tcttttagga cttgccaagg ctaagaatgc acggatgctg attgcatcga catccgaggt
     3841 atatggtgac ccgctggtgc atcctcagag ggaggattac tggggtaatg taaatccaat
     3901 cgggcccaga ggggtgtatg acgaagcaaa gcgttttcag gaagcaatga ctatggccta
     3961 ccataacttc catgggctgg aaacacgtat tgtaaggatt ttcaacacct acggacctcg
     4021 catgcgtgtc aatgatgggc gcgcgttacc tgcattcttt agtcaggcaa taaaaggaca
     4081 aaacctgacc gtctttgggg acggcagcca gacaagatcg ttttgttatg tcgatgatct
     4141 ggtagaaggt atctacagac ttttattgtc ggattatcat cttcctgtca acattggcaa
     4201 tccggaggaa atcaccctca accaatgtgc cgatgaagta atcgagatcg taggaaatcc
     4261 ggaagtcaaa attgagtacc atgatttgcc gaaagatgat cctaaggtaa ggaggccgga
     4321 tattacgctg gcaaggcaat tgctgggctg ggagcctaca gtcagcagga atgagggatt
     4381 aagagctacc ttcgaatatt tcaagaaagc cctgtcataa gctaaaatat taaataacag
     4441 aaaaatggta ttaaatttgc agcctgaaat gatgaatggc ttttaatgct ataaccaact
     4501 tacatatatg aataagatat atatattatt tcttttactt attattcctg cattgtcata
     4561 ttcgcagata gacaataagg acatgaaaat gttgatgttg caaaacagtg gcaagataga
     4621 caaaataaaa gcggatataa aggcaaaggg cataacagaa gcagaacttt caagtaaatt
     4681 aaagcaaaaa ggaatcgacc ttcagaacct gcaacccgag cagattccaa cgctggaatt
     4741 gatggttgac gaagccattc gggaaattga aaatgaaaaa ataaaggcca aatctgagat
     4801 tgaaacgaag ccgtctacgt cggacaaaaa agaaaaggaa gttgacctgg ataatttcga
     4861 attttccagg aatttcacca aatccattga agaatccttt gatcttaagg gcaataagca
     4921 gaaaactgcc cgttttgtaa gtgactcatt aaaagcggcg gacaccattc catttgcaat
     4981 cttcggccag caaatattca aata
//
LOCUS       DAOQQN010000240         4996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7688_length_4996_cov_6.342238, whole genome shotgun sequence.
ACCESSION   DAOQQN010000240 DAOQQN010000000
VERSION     DAOQQN010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7688_length_4996_cov_6.342238"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="PLG24_09230"
     CDS             <1..988
                     /locus_tag="PLG24_09230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HMZ25116.1"
                     /translation="DISAILAGNIDSKIENKPSTPEAKSDSGIPQTVPAQVDATNDSH
                     GRIKASPLAKAIATEKGIDLSKIEGSGDGGRIIKHDVENYQIQPGSSTQTSTPIRSAG
                     QAYKDIPLSQVRKTIARRLSESKFGAPHFYLTIEVDMEHAIAAREALNKKGDVKISYN
                     DLVTKATALSLAKHPQVNSSWMNDFIRENYVVNIGVAVAVPDGLLVPVVRDANFKTLS
                     ELNQEIKTLAAKAKEKKLSLEEMQGNTFTISNLGMFDIDNFTAIINPPDACILAVGSI
                     VSKPVVKNGAIVPGNTMKLTLSCDHRVVDGAVGAQFLQTLKSYLEQPLNMLL"
     gene            complement(1458..1940)
                     /locus_tag="PLG24_09235"
     CDS             complement(1458..1940)
                     /locus_tag="PLG24_09235"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HMZ25117.1"
                     /translation="MEKKIISFEYQIIPDLDGLESDDRALLKRASEALEQAYAPYSEF
                     KVGASLIDSEGNVFIGANMENASYPICICAEGALLTAYNTSGSKAPIRTVAITATSSS
                     HVIDHPIAPCGACRQMLSEMELKQGQAIRLLLQGSEGPVYIIDKISDVLPLGFSGTEL
                     "
     gene            2068..3414
                     /locus_tag="PLG24_09240"
     CDS             2068..3414
                     /locus_tag="PLG24_09240"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HMZ25118.1"
                     /translation="MSDSKSKKTITLFGAGRSATFLIEYLLKHAPSEGWQIILADSNP
                     EVAFSKLGGNPNGSAVSCDINDAVKRRELIAASDFVISMLPAFLHILVAKDCLMYNRD
                     LATASYLSDELKEMEDEIKAKGLVFANELGLDPGLDHMSAMKIFDEIKRKGGQITSFK
                     SFTGGLVAPESDDNPWHYKISWNPRNVVLAGQCTSQYMESGNVHYVPYHRLFRISEDI
                     KVIGHGTYSMYVNRDSIKYIDLYGLEGIQTMIRGTFRGENYCDAWAVLIDLGLTDNST
                     RMSLNKNFTLREYIIGFLSGSGNNYKHRIESTIGRKISDRTFEQIQWLGLFSNDTCPL
                     QHPTPAEILEYIIVNKWVLKPGDKDLVVMQHEIEYLLGDQTHTLYSTLYRTGTDDHHT
                     AMSELVGLPLAIYTKLRLRGNFGTPGCILPTTEDLYKPILEELEDIGIKFIESHKI"
     gene            3411..4739
                     /locus_tag="PLG24_09245"
     CDS             3411..4739
                     /locus_tag="PLG24_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25119.1"
                     /translation="MTTQGSDKTKLRFRDDPVKVYQGFNVARQSAIILASIILAKSAM
                     STENIGKYETLLFLGQFISLFWVNGLSQAFIAIYPKVAEVERKSFVSSTFTIFLGLAF
                     ASSLVLIAGRPLLLPFLIESRTIDGFYWFCLYSMLNIPSMLIPSLMMLQGNSRELLRF
                     SMFYAIGYTMVFVLNMVFGGRLINLLILLNVFSFALLVMSGAMSLSLDLRAYRLKWVK
                     RLFLIGSPLVGYAILNGLAPLFDSWLVQRFYTDKAVFAIYKYGAREFPLTVTLAIGLG
                     MALVPMLGRNPTSGMEQIKQRSRKLFPIVFITSALLILTSKYFFPIVFNNNFTSAAAV
                     FNLYVLIIISRMIYPHTIMMGLKRTKALLYISITEIFIHITSSLLLGLKFGLVGIAGG
                     AVIAFTIERLLQVVYLKWKRGINVNQYVQLKPYILYSLIVIVAWLLTIYL"
     gene            complement(4831..4913)
                     /locus_tag="PLG24_09250"
     tRNA            complement(4831..4913)
                     /locus_tag="PLG24_09250"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4878..4880),aa:Leu,seq:taa)
BASE COUNT         1540 a         1055 c          992 g         1409 t
ORIGIN      
        1 ggacatatca gcaattcttg ccggaaacat tgattcaaaa attgaaaaca agccctcaac
       61 ccccgaagct aaaagcgatt caggaatccc acagacagtg cctgcccagg tagatgccac
      121 caatgatagc catggtcgta ttaaggccag cccattggca aaagccatcg caacagaaaa
      181 aggtattgat ctctcaaaaa ttgagggcag tggagatggt ggacggatca tcaaacatga
      241 tgtagagaat tatcaaattc agcctggctc atcaactcaa acttctacac ctataaggtc
      301 tgcaggacaa gcatataaag atatacctct ttcacaagtt cgtaaaacca tagccagacg
      361 tctcagcgaa agtaaattcg gtgcgccaca tttctacctt accatagaag tggatatgga
      421 gcatgcaatt gccgcccgag aagcgttaaa taaaaaaggt gatgtaaaaa tttcatataa
      481 tgacctagta acaaaagcga ctgcgctctc tctggccaaa cacccgcaag tcaactcaag
      541 ctggatgaac gactttatac gtgaaaatta tgtcgtcaac atcggtgttg cagttgcagt
      601 tccggatgga ttgcttgtac cggtggtaag ggatgctaat tttaagacat tatcagaatt
      661 aaatcaggaa attaaaacgt tggcagcaaa agccaaggaa aagaaattga gcttggagga
      721 gatgcagggt aataccttca ccatttccaa tctgggaatg tttgatatcg acaactttac
      781 agcaatcatc aatccacctg acgcatgcat tctggctgtc ggaagtatcg taagcaaacc
      841 tgtcgttaaa aatggtgcaa tagtgccagg taacacgatg aagcttactc tatcctgtga
      901 tcacagagtt gtagatggtg cggttggtgc ccaattcttg caaacgctga aatcttatct
      961 tgaacagcct ttgaacatgc tgctttaaga ggaggtttct caggatgttt acatttattt
     1021 aggcataggt tttaatccaa cctcatgatt cgaattcaat aaagccaatg gattccggtt
     1081 cttacttgga ggaattaacc ctaacagcca ccaccttatt gtcttatccg gatacaaagt
     1141 gctgattcat aagtcaaaca cctaaggtat tattgaaatc aagggtagaa attatatacc
     1201 ggaagccatt tatacatttc attatatcgc cgtttctttc tgtaaattaa ttagtataaa
     1261 atccaataaa cacaaagatt aaggatataa ttttcagttc aaatcaacct caactttatt
     1321 gataaactac ctataaaata ttttctctta aaacatagaa ttaccttatt cgatcggaca
     1381 agaatcttct atattatatt caatagctat tccgcccccg aaacttccat ttaaagacgg
     1441 tataatgttc attttatcta aagctcagtc cccgaaaaac ctaatggcaa tacatcgctt
     1501 atcttatcga tgatatacac cggcccttca ctcccttgca gtaataggcg gatagcttga
     1561 ccttgcttta gttccatttc actcaacatc tgacgacatg cgccacatgg ggcaatcggg
     1621 tggtcgatga catgggatga ggaggtggca gttatggcta ctgtacgaat aggcgctttt
     1681 gatccactgg tattataggc agtgagaagg gcgccttccg cacaaatgca tatcggataa
     1741 gaagcattct ccatattggc cccaatgaat acgtttcctt ccgaatcaat caaagatgca
     1801 cctaccttaa attcagaata aggagcataa gcttgctcca atgcctcact tgctcttttc
     1861 agaagtgccc tgtcatcact ttcaagccca tccagatcag gtattatctg atattcaaat
     1921 gatataatct tcttctccat atagaaatgt tggattaaat ataataaaat agttgatgat
     1981 gttaatataa aaataccagc aaattcaatc aactaaggtt aaattaataa attcgcacct
     2041 tcaaaaccag attttcaacc ggctctcatg tcagattcta agtcaaaaaa aacaatcact
     2101 ctctttggtg cagggcgatc tgctacattt ctaatcgaat acctactcaa acatgcgcca
     2161 tctgaaggtt ggcaaattat ccttgcagac agcaaccctg aagtagcttt ttccaagctc
     2221 ggcggaaatc caaacggctc agctgtctcc tgtgatatca atgatgccgt taaaaggaga
     2281 gaattgatag ccgcatcgga ttttgttatc tccatgttgc ctgccttctt acatatctta
     2341 gtagctaagg actgtttgat gtacaacagg gatttggcaa cggcatccta tctgagcgat
     2401 gaactaaaag aaatggagga tgaaatcaaa gccaagggcc ttgttttcgc caacgaattg
     2461 ggtttggatc ccggtctgga ccatatgtca gccatgaaaa tctttgacga aatcaaaaga
     2521 aaaggtggtc aaatcacttc attcaaatcc tttaccggag gccttgttgc acctgaaagc
     2581 gatgacaacc catggcatta taagatcagc tggaatccca ggaatgtcgt cctcgcaggt
     2641 caatgcacat ctcaatacat ggagagtggc aatgtacact atgtccctta ccacagactt
     2701 ttcaggatat cagaagatat aaaggttata ggccacggca cctattctat gtatgtcaac
     2761 cgagattcca tcaaatatat cgacctttat ggactggagg gcattcaaac catgatcaga
     2821 ggaacattca ggggggaaaa ttattgtgac gcctgggctg ttcttatcga cctcggattg
     2881 actgacaatt cgacaagaat gagtttaaat aaaaatttca cgctgagaga atatattata
     2941 ggcttcctgt caggatccgg aaataactat aagcaccgaa tcgagtctac aataggcaga
     3001 aaaattagcg accgcacatt tgaacaaatc caatggctgg gcctcttcag taacgatact
     3061 tgtcctctcc agcatcctac tcctgctgaa atccttgaat atatcattgt aaataaatgg
     3121 gtactcaagc cgggagataa ggatctggtt gtcatgcaac acgaaattga atatctgctg
     3181 ggagatcaaa cccatacact ttactccacg ctttatcgta ccggtactga tgaccatcat
     3241 accgcaatga gcgaactggt aggcctgcca ttagctatat atacaaagtt gcgcctacga
     3301 ggaaattttg gaacacccgg ctgcatcctc cctacaaccg aagacttgta caagcccatc
     3361 ctggaagaac tagaggatat aggcattaaa tttattgaaa gccataagat atgacaacac
     3421 aagggtcaga taaaactaaa ttacgattta gagacgaccc ggtcaaggtc tatcaaggct
     3481 tcaatgtagc acgtcaatcg gcaattatcc tggctagtat cattctggcc aaaagtgcca
     3541 tgagcaccga aaacattggt aaatatgaaa ctctcttatt tttggggcaa tttataagtc
     3601 ttttttgggt caacggcctc agccaggcat ttatcgctat ctatcctaaa gttgccgaag
     3661 ttgagagaaa atcattcgtc agttctactt ttactatatt tctcgggctt gcctttgcaa
     3721 gttcactggt attaattgcc gggaggcctt tactgctacc gtttttgatt gaatcacgga
     3781 caatcgatgg attttactgg ttttgccttt acagcatgct caacatacca tccatgttaa
     3841 ttccctctct aatgatgttg caagggaaca gtcgggagtt gttgcgcttt tcgatgtttt
     3901 atgcaatagg ctacaccatg gtattcgtat tgaatatggt atttggtggt aggcttatca
     3961 acttactcat attgctcaat gtattttcct tcgccttgct ggtcatgtcc ggggcaatgt
     4021 cactgagtct tgacttacga gcatataggc taaaatgggt aaaaaggttg ttcctgatag
     4081 gaagtccttt ggttggatat gcaattctga atggtctggc tccacttttt gattcctggt
     4141 tggtacaacg attctatacc gacaaagcag ttttcgcaat atataaatat ggtgcgcgtg
     4201 aatttcctct gacggtaaca ctggcgattg gattaggaat ggcacttgtt cccatgctgg
     4261 ggagaaatcc gacatcaggg atggaacaga tcaaacaacg ctcaagaaaa ttattcccta
     4321 tcgtatttat aacttcagct ctacttatcc tcacttcaaa gtatttcttt ccgatcgtat
     4381 tcaataataa ctttacttca gcagctgccg tatttaatct atacgtacta atcatcatca
     4441 gtcgcatgat atacccccat accatcatga tgggattaaa gcggaccaaa gcattattat
     4501 atatttcgat taccgaaatc ttcatacata ttacgtccag tttgttatta ggcttaaaat
     4561 ttggcttagt tggaattgca ggtggagctg ttatagcatt tacgatagag agactactgc
     4621 aagtcgtcta tttgaaatgg aaaagaggca tcaatgtcaa tcagtatgtg caattaaaac
     4681 cctacatcct gtattcatta attgtaattg tcgcctggtt gttgacgata tatctatagg
     4741 aaaaagatat agacccaaaa atctttttaa aaaggattta gaaattcaaa ttgcaatgtt
     4801 ctaccttaag gctatgtggg tgaaaaagtg tacccggagc gggacttgaa cccgcacgac
     4861 ttcgcagtca aaggatttta agtccttcgt gtctaccatt ccaccacccg ggctaaagta
     4921 taatagagga aagtaaaaat ggagcgggaa acgagactcg aactcgcgac cccgaccttg
     4981 gcaaggtcgt gctcta
//
LOCUS       DAOQQN010000241         4996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7689_length_4996_cov_5.370573, whole genome shotgun sequence.
ACCESSION   DAOQQN010000241 DAOQQN010000000
VERSION     DAOQQN010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7689_length_4996_cov_5.370573"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PLG24_09255"
     CDS             complement(<1..495)
                     /locus_tag="PLG24_09255"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain-containing protein"
                     /protein_id="HMZ25120.1"
                     /translation="MGPTEITIYLILTTAVVIVLIGGIIFYLFLYRHKTIVHKVEKEN
                     LTHQHQLEILNTEMESQRHTAEHIGREIHDSVAQKITLASIYIQKLQFGPGKEIASEE
                     FSTINDILSKALQELRNLSKDLSGDALSKLPLELLILEECKVVNNSGKCTVKFYSNDF
                     PPCDI"
     gene            complement(1140..1832)
                     /locus_tag="PLG24_09260"
     CDS             complement(1140..1832)
                     /locus_tag="PLG24_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25121.1"
                     /translation="MAKNLIILLLTAVVVYCMLEKYSYINNKSSKCLDTICKTYTFDD
                     LTTTVSGAALKAMSESYAMDKSKSMVHNQNGTSVIDTSDATSIVFDLSTIKALIYMME
                     ETKCKNKCDDKVRMGIRYYYIKYPDNFEVPPYSNGGLAGMDSKYHNKHSLAMVPVVQF
                     EGNKDWQDFDLRRSSPTDCFPAFDLQSIEEEGRDPKSNERTFVPLVMPLDAGENHGGM
                     APPPYPGTFPIK"
     gene            2116..>4996
                     /locus_tag="PLG24_09265"
     CDS             2116..>4996
                     /locus_tag="PLG24_09265"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HMZ25122.1"
                     /translation="MKKTILFQVFTTISLLLIGSISMNAQNFSNDLHDLTTAIDKDKS
                     QSEPLFTPFVYVRQVANVRQDIGMSRYNELKIDKTSLREIQKEKPGTMTMNFPVSNGT
                     IVKLKLTKNNILNDDFSIGINDQGKMAEDVDLGLHYRGTGLLDGKEIVCSFSFFKNNI
                     EGMISGHEQINITSIKTPDNTEAISFYQKDVTEEQPLGCGTVHDGVDYLPEQLQAPLE
                     TREAKCVRIWWECNYNVYQNLGNDLTNTIIFNLTLFNAYHTLYLNESIKVLLHHLEVW
                     TTTSPYTNPSDPMVDDRDEFLIKFQTNRAASFTGDLAGLITQNNIGGVAAGFAGLCNV
                     NRSLSMCISGMYGNSVGTYPSYAFNVYITTHEFGHLFGSRHTHACVWNGDNTAIDGCA
                     GYVEGSCPLPGYPSGGGTIMSYCWNPHWIDFTLGFGPQPGNVIRNSVNVAGCLISCCN
                     PAFNPSITSNSPVCQGGTLTMSVSGGDLFAWTGPNGFKSSSSSPSISNITMAQAGTYN
                     LIVTNAEGCIASVSTNVIVNPIPIATANASPNPICAGQTLQLSSSGGTSYSWTGPAGF
                     SSGQQNPTRANVTTLMSGNYIVNVTSAQGCSSSANVNVTVNPLPIATASASPASVCEG
                     QSINLSATGGGSYAWSGPGGWTSNLQNPTRSNASVAMTGTYTVTVTSGAGCASVASVD
                     VTVNPLPVASAKADPNPICSGHVAHLYAEGGVSYSWSGPNGFTSNQQNPNLGLVQINQ
                     AGVYTVTVTNAEGCSSTASVNLVVNQSPTGTIGISTNFACVGTQVQLTASGGQNYLWL
                     GPGGFTSTLQNPLINVTSHVQGGTYWVTISNNNGCYVQLSVKLEVGYPPAVTATHDQS
                     TACTGSTLLFSGSGVGTYLWTGPGGFTSTQQNPKITNVSPANSGTYTLVVTAANGCSA
                     TAATTVNVVAPPSMSAWADDYDICEFSTAYLHATGASSYEWTGPWG"
BASE COUNT         1462 a          994 c         1072 g         1468 t
ORIGIN      
        1 gatgtcacat ggaggaaaat cattggaata aaatttgacg gtgcacttgc cggaattgtt
       61 gactacctta cattcctcta aaatcaacag ctcaaggggc agtttgctta gagcgtcacc
      121 ggaaagatct tttgaaaggt tgcgcaactc ctggagtgct ttggaaagta tatcattgat
      181 agtagaaaat tcttctgatg caatttcttt tccgggacca aattgcagtt tttgtatata
      241 gattgaggca agggtgattt tctgtgctac actgtcatgt atctcacgtc cgatatgttc
      301 ggcggtatgt cgttggcttt ccatctcggt attcagaatt tctaattggt gctgatgcgt
      361 taggttttct ttttcaacct tatggacgat tgttttgtgc ctatatagaa ataagtagaa
      421 tataatccct cctatcaata caattacgac agcggtagta agaatcagat atattgtaat
      481 ttcggtcggc cccatttaaa ggatattata aagcatatat acatgatata attcagacaa
      541 aactgaagga tcaccaatcc attcccaatt tcaggaaaat tcaccagata actccggatt
      601 agccaatagg gcaaagaacc ggcaaagaat agcaacaaac caactgatac ccaaaacatc
      661 atggaagtct tgaattgaat gagttcatcc gatttgataa aaacagcaat aaattgaaag
      721 atggcgagaa taatcatgat ggccccaatc gtgtatgaaa tgtcatgaaa ccaggggttg
      781 gtatgaaagg tctggaaatg gtcgaacacc aatgccgtta tataaattaa agcaaagtaa
      841 atgggaattt tttggacttt caagccctca aaatatttat aaaatagcca gaagtaaaac
      901 aggaattgac cgggtatgat aatatattta tataatgcta aattcacatc aggcatccca
      961 aatcccagat tgaatatttc ggcggtaatt tcaaagataa aaatcagcag cagaaagggg
     1021 gggaagaatt tccaataggt gtctttaatt tttttttggt aataaaatcc cgtgactgct
     1081 gccgacaatt caaataagtt cagtataatt aagaaaatga tataagctga cttcattggc
     1141 tatttgattg ggaatgtgcc cggataaggc ggaggtgcca tgccgccatg attctctccc
     1201 gcatcaagtg gcattacaag agggacgaag gttctctcat tgcttttagg atctctgcct
     1261 tcttcctcaa tggattgcaa atcaaatgcc gggaagcaat cagtgggaga ggatctcctc
     1321 aaatcaaaat cctgccagtc tttattgccc tcgaactgaa ctaccggaac catagctaag
     1381 gaatgtttat tgtggtattt gctatccata ccggccagac cgccattgga ataaggtggg
     1441 acttcaaaat tgtccggata tttgatgtaa tagtatcgta tccccattct gaccttgtca
     1501 tcgcatttgt ttttacattt tgtttcttcc atcatataga ttaaggcttt aatggttgaa
     1561 agatcaaaga caatcgaagt cgcatcactg gtatcgatga cagatgtccc attttgatta
     1621 tggaccatgg attttgactt gtccatggca tatgattctg acatagcttt cagtgcggcg
     1681 ccagaaaccg ttgttgtcag gtcatcaaaa gtgtacgttt tgcaaattgt gtccagacac
     1741 ttgctggatt tattgtttat atatgaatat ttctccaaca tacaatacac cacaacagca
     1801 gttaataata gtattattag atttttagcc atgattatta cattttatag tgtttaaata
     1861 atttgctaaa atagaataat attagcggac tccacaccgt gaaatcacgg tttttaagtc
     1921 gtgatattac aatttcaaaa cagttaataa actcttggat ttttggtctt ttggtgttaa
     1981 atttgtatca tcctttcagc aaaattaatc cccacctaac acaggagtca ttattaatgt
     2041 atgatgcctg cgggatcgaa tgattattat tttttgagcg ccctgattat ttattcatct
     2101 ttaaaatatt tcattatgaa aaaaacaatt ctattccagg tttttacaac catttcgcta
     2161 ttgctgattg ggtccatttc aatgaatgcc caaaatttta gcaatgattt acatgatttg
     2221 accacagcga ttgacaaaga taaaagtcaa tcagaaccgc tttttactcc ttttgtctat
     2281 gtcaggcaag ttgccaatgt aagacaggac attgggatga gccgttacaa tgaattaaaa
     2341 attgataaaa cttcactgag agaaatccaa aaagaaaaac ccggaaccat gaccatgaat
     2401 tttccggttt ccaatggcac gatagtcaaa ctgaaattaa cgaagaataa catattgaat
     2461 gatgatttct caatcggcat caatgatcag ggtaagatgg cagaagatgt ggacttagga
     2521 ttgcattata gaggtacagg gttgttagat ggaaaagaga tcgtgtgctc cttcagcttt
     2581 ttcaagaata atattgaagg gatgataagt ggccacgaac agataaatat cacatccatc
     2641 aagaccccgg ataatacgga agctatctca ttttatcaga aggatgttac tgaagagcaa
     2701 cccttgggct gtggtacagt gcatgatggt gtggattacc ttcctgaaca attgcaggct
     2761 cccctggaga caagggaagc caagtgtgtt aggatttggt gggaatgtaa ttataacgta
     2821 tatcagaact taggcaatga tttgaccaat accatcattt tcaaccttac gttattcaat
     2881 gcttatcata cattgtattt gaatgaatcc attaaagtgt tactacatca tctggaagta
     2941 tggacaacaa caagccctta tacaaatcct tccgatccaa tggtagatga cagagatgaa
     3001 tttttaatta aatttcaaac taatcgtgct gcatctttta cgggtgatct tgcaggattg
     3061 ataactcaaa ataatatcgg cggagttgct gctggattcg caggtctttg caatgtcaac
     3121 aggtcgttaa gtatgtgtat aagtggaatg tatggtaatt cagttggtac ttaccccagt
     3181 tatgctttca atgtatatat cacaacccac gagtttggac atctgtttgg ttcacgtcat
     3241 actcatgcct gtgtatggaa tggtgataat acggctattg acggatgtgc agggtatgta
     3301 gaaggaagtt gtccgttgcc cggatatcca tcgggaggag ggacaataat gtcttattgc
     3361 tggaatcctc attggattga ctttactttg ggctttggtc cacagccggg aaatgtcatc
     3421 aggaatagtg tgaatgtcgc aggatgtctt atttcttgtt gcaatccggc cttcaatcct
     3481 tctatcacta gcaattcgcc cgtatgccag ggaggtacgc ttacgatgtc agtgagtggt
     3541 ggagatctat ttgcctggac aggccctaat ggatttaagt catcctcttc atcaccttcc
     3601 attagcaata ttacaatggc tcaagcaggt acttacaatt taatcgttac taatgcagag
     3661 ggatgtattg catctgtttc cacaaacgtc attgtaaatc ctattcccat tgcaacggca
     3721 aatgcttcgc caaatcctat ttgtgccgga caaacattgc aactatcatc atcaggtgga
     3781 acctcctatt cctggacagg ccctgccggt tttagttctg gacagcaaaa tccaaccaga
     3841 gccaatgtaa ctaccctgat gagcggtaac tacattgtca atgtcacctc tgctcagggg
     3901 tgttcttcat cggccaatgt caatgtaaca gttaacccct tgccgattgc aacagcttca
     3961 gcgagtccgg cttctgtctg cgaggggcaa agcatcaacc tgagtgcaac aggaggaggg
     4021 tcgtatgcct ggtcaggccc cggtggttgg accagcaatc tgcaaaatcc caccagaagc
     4081 aatgctagtg tagcaatgac aggaacttat actgtgacag tgacttcagg ggcagggtgt
     4141 gcctctgttg cttctgttga tgttactgtc aatccactgc cggtagcttc tgctaaagcg
     4201 gatccaaatc caatctgttc aggacatgtt gcccacttgt atgccgaagg aggtgtgagc
     4261 tactcatgga gtggcccgaa tggctttacc agtaaccagc agaatccaaa tttgggtttg
     4321 gtgcagatca atcaggctgg tgtctataca gtgactgtaa cgaatgctga aggctgctct
     4381 tctactgcat cagtcaactt agtggtcaat cagagtccaa ccggtacaat cggcatttca
     4441 accaacttcg cctgtgtggg aacacaggta caacttactg cctccggtgg tcaaaactac
     4501 ctatggctgg gccccggtgg atttacttcc acactacaaa atccgttgat caatgtgact
     4561 tcacatgttc aaggcggtac ttattgggtg accatatcca ataacaatgg atgctatgtt
     4621 cagttgagtg taaaattaga agtgggatat ccgccggcag tgactgccac tcatgatcaa
     4681 agcacagcct gtacaggcag taccctgctg ttttccggtt cgggtgttgg cacttatcta
     4741 tggacgggac cgggtggatt tacgtctaca cagcagaatc cgaaaatcac caatgtttct
     4801 cctgccaaca gcggcaccta tactcttgta gtcactgcag ccaacggatg tagtgcgaca
     4861 gcggccacaa cagtcaatgt tgtagcacca ccaagtatgt ccgcttgggc agatgactat
     4921 gatatttgtg agttttcgac cgcttatctc catgcaactg gtgcgagttc atacgaatgg
     4981 acaggaccat ggggtt
//
LOCUS       DAOQQN010000242         4995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7692_length_4995_cov_6.236640, whole genome shotgun sequence.
ACCESSION   DAOQQN010000242 DAOQQN010000000
VERSION     DAOQQN010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7692_length_4995_cov_6.236640"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PLG24_09270"
     CDS             complement(<1..375)
                     /locus_tag="PLG24_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25123.1"
                     /translation="MKNALKNFKLSSINRYAGILIAAAIMTFAFQSCSKESIDPTATA
                     SYKVLNANMRKLWSDHMQWTYSTVDAFFNNPDGLNGPLNRLLQNQKDIGAAIIPYYGE
                     DAGGQLTKLLTDHITGAVPVLTA"
     gene            complement(527..1222)
                     /locus_tag="PLG24_09275"
     CDS             complement(527..1222)
                     /locus_tag="PLG24_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25124.1"
                     /translation="MQTTITSNKIKGTKQKESIIIKTQIKRFAAFLILLVSLQSCHKD
                     DIETNPLDSTKYKIFSNSMRKLWSDHATWTRNLIIDIAEDAPGTAVDFTRLLKNQEDI
                     GEAIKPYYSEEAGDKLTYLLKEHIVIARNVFTATKSRNTDDLNLMIDSLYENGDAIAM
                     FLSTANPMNWKSHHMKEHMKEYIDLTLSEATNHLNGNYQGSIEAYDEIYSQLLHLADQ
                     LSSGIALQFPEKF"
     gene            1840..2367
                     /locus_tag="PLG24_09280"
     CDS             1840..2367
                     /locus_tag="PLG24_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HMZ25125.1"
                     /translation="MNRRTLVSFDWALKRLLRNKANFNILSGFLSELLFDDICVEEIL
                     ESEGNQDSDRDKFNRLDLLCPNQRNEFIIIEVQYHDESDYFHRCLYGTSKVITEYINK
                     GEPYHQVKKVYSVNILYFDLGHGDDYIYVGKTKFTGLHNKSNLTLSPTQQKKFDKSEP
                     FEIFPEYILLKINNI"
     gene            2486..2722
                     /locus_tag="PLG24_09285"
     CDS             2486..2722
                     /locus_tag="PLG24_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25126.1"
                     /translation="MDKVSKQQYDQYLKNMRISQDALDTALEKGLIKGRLQEKIDIVL
                     NCLSNGIDIHLIANITNLSEEEVEKIIREHKDQI"
     gene            3152..3469
                     /locus_tag="PLG24_09290"
     CDS             3152..3469
                     /locus_tag="PLG24_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25127.1"
                     /translation="MKSFHMIFIGLYCLAALYFTYLSFTTMFVYFSNKSMGFQESFLE
                     PGKNLILALLFIFFTAASWFLLKSPNYHKLGMIVFYIPIVIIGLYILWAVIMLIGSGG
                     KWN"
     gene            3589..4875
                     /locus_tag="PLG24_09295"
     CDS             3589..4875
                     /locus_tag="PLG24_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HMZ25128.1"
                     /translation="MELNYVSAAEALSLIKSNSRVFLHGSACTPVYLLEELAKNAGNY
                     KNVELVFISLQGPEMEIAKPEYADSFHVNSLFVSAPVRGAVNSGFADFVPVFLSDIPD
                     LFNDGIFPIDVAIIHVTPPDDHGYCSMGVSVDIARAAVNNAKKIIALVNPRMPRTMGD
                     GMIHYKHIDKMVFHPSELPEVDYSSKVTEIDRMIGRNIAEMVEDGSTLQLGIGTIPDS
                     VLTYLGNHKDLGVHTEMLSDGIIDLFLNNVINNRKKRIHPNRMVTSFALGTRRLYDFV
                     DNNAGLAFLDIDYVNNPSVIAKNDKVVAINSCIEIDLTGQVCSDSIGTYQYSGIGGQM
                     DFIRGAALSKGGKPIIAINSRTRKGQPRIVPTLKPGAGVVTSRGHVRYVVTEYGVVNL
                     FGLNMRQRAKALIEIAHPDDREMLHQSAYERFKIFL"
BASE COUNT         1412 a          953 c          967 g         1663 t
ORIGIN      
        1 agcagtaagc accgggacgg ctcctgtgat gtgatcggtc aataatttag tcaactgacc
       61 acctgcatcc tctccatagt atggaatgat agcggctcca atatcttttt ggttttgcaa
      121 tagcctgttc aaagggccat tcagcccgtc cggattgttg aagaatgcgt ccacggttga
      181 atatgtccat tgcatgtggt cactccagag ttttctcata ttggcattga gtaccttata
      241 gctggctgta gcagtcgggt caatactttc cttgctacaa ctctggaaag caaatgtcat
      301 aatggctgct gcgataagga ttcctgcata tctatttatg ctgcttaatt tgaagttttt
      361 taatgcgttt ttcatgttta taaattttaa atgttattaa tttttgtact ttgattttgg
      421 gggtgctgat gtttagctat tgatttcccc ggccgattca tgtcagcttc ttgttttcag
      481 tgattttagt gcacaaatta ttttcgctga aacctgtaag tatggtttag aatttttcgg
      541 gaaactggag ggcgattcct gaggataact ggtcggcgag gtgaagtagc tggctgtaaa
      601 tttcatcgta tgcttctata cttccctggt aatttccatt caaatggtta gttgcttcgc
      661 ttagtgtcag atcgatatac tccttcatat gttccttcat atggtgagat ttccagttca
      721 tcggattggc agtactaaga aacatcgcta ttgcatcgcc attctcatac aatgagtcaa
      781 tcattaggtt caggtcatcg gtatttcttg attttgttgc ggtaaataca tttcttgcta
      841 ttacaatgtg ttctttcagt aaatatgtca gtttgtcgcc ggcttcttcg gaatagtatg
      901 gcttgatggc ttctccaata tcttcctgat tcttaaggag tctggtgaaa tcaactgctg
      961 tgccgggagc atcttctgcg atatcaatta ttaaattcct tgtccatgtt gcatgatcac
     1021 tccataactt tctcatgcta ttgctgaaga ttttatattt tgtactgtct aatgggttgg
     1081 tttctatatc gtccttgtga cagctctgta gcgataccag aaggatcagg aatgccgcaa
     1141 aacgtttaat ttgagttttg attattatag attccttttg ctttgtccct tttattttat
     1201 ttgatgttat tgttgtttgc attttattag agtattatgg ctaataagag gttactattt
     1261 attgaatttt attacggatt ttttctctga tttattcgta ttttaatatc caatatgtat
     1321 tcacgacaga taatcaaatt gatgtatctc attatttcag ttctttcctt tcgtaacttc
     1381 ttacattttg attagagtat taaggataaa aaaaggttac aggtattaga aaaatttttt
     1441 tgaatgatta cgtataagac aacttatttt gttgaatagc cttcaaaaaa tgacaaattt
     1501 catccttaaa aggaccatgc tcaggtcggt ttgtatcaaa aggtgcaggt tgacaatcgg
     1561 taaagttttg tagatcttat aaaaggtttc ctgtttcaca ggggcgccta cgattgccag
     1621 aaacgatcaa tatattccca tctcaaccga tcatggatat tttggaatta atggctggct
     1681 gtctccgaat attcaatatc agaagtggaa acaaatgcca tccacattgt ggtaaaacta
     1741 aataccttac ttttatatta ctaaaatagt ttttaaaaat tcttttcttt ttgaatcttc
     1801 aaatcatttc tttatcttcg tttacttaaa tcacctaaaa tgaatagaag aactttagta
     1861 agtttcgact gggcattaaa acgcctcctt cgtaataagg ccaatttcaa catcttaagc
     1921 gggttccttt cagaattact ctttgacgat atatgtgtcg aagagatatt ggagtccgaa
     1981 ggcaatcaag attctgaccg ggataaattc aacaggcttg accttctgtg cccaaaccaa
     2041 cgtaatgaat ttattatcat cgaagttcaa tatcacgatg agtcagacta cttccatcgt
     2101 tgtctgtatg gcacctccaa ggtcatcacg gaatacatca acaaaggaga accatatcat
     2161 caggtcaaaa aagtatattc ggttaatatt ctgtatttcg acctgggaca cggcgacgat
     2221 tatatttatg tcggaaaaac caaattcacc ggattacata acaagtccaa cctaacttta
     2281 tcgcccaccc aacaaaaaaa atttgacaaa tcagagccat ttgaaatttt tccagaatat
     2341 atattgttga aaataaataa catttgataa tgtctccaga aatacgcttg atgaatggat
     2401 ttatttctta aaaaactctg aacttccgca taagtttcat gctaaaggct tatcagaagt
     2461 atcagaaaca ctgaaatacg acaaaatgga taaagtttca aaacaacaat atgaccaata
     2521 tctcaaaaat atgagaatct ctcaggatgc tttggatacc gctttggaga aaggtttaat
     2581 caaaggtcgc ctgcaggaaa aaatcgatat tgtgctgaat tgcctttcta atggcataga
     2641 cattcacctg atagctaata taaccaacct ttctgaagag gaagtcgaaa aaataatcag
     2701 ggagcataag gaccaaattt aatttcctcc tatcttgtat gaattttact ctaaaaaagt
     2761 atttaagctt ataagccacc ctttaagtaa atctttattc atgccggaga gggtcatgcc
     2821 ttagcaggat tatatcgact ctcctccgat acctgacagg aagatttatt tattcgccgg
     2881 aaatcaggat agagccatta gagttgccaa ttatttattc catttttagc acagacaaag
     2941 caaaaggaat caacccgtat aataggctta ttgtcaccct catcaaaagc ccatttcatc
     3001 ctataaccgg atacatggac tgcgtccatg ttatactccg gaaaatgggg tgcagttact
     3061 gggttagtta ccatccaccc aaagtccctg gacatagatt tttttttaaa tatgcgcatt
     3121 ttgcgttatc ttgtatcata tttccactgt tatgaagtcg ttccatatga tttttattgg
     3181 cctgtactgc ctggccgcac tttattttac ctatttgtca tttacaacaa tgtttgttta
     3241 tttttccaat aaatcaatgg gattccagga gtcttttctg gaaccgggca agaatcttat
     3301 tttagcactt ttattcatct tttttactgc tgcttcatgg tttttgctga agagtcccaa
     3361 ttatcataaa ttagggatga ttgtatttta tattccaatt gtaataattg gtttatatat
     3421 cttgtgggct gtcatcatgc tgataggatc aggaggtaaa tggaattgat ttggcatatt
     3481 gggcctcttt tttttgtact aaaggcatat tgttgaagtt attgatgata tagcataaaa
     3541 tttcatttct ccgaaaattg ctcttatttt tggcgccaaa attttgaaat ggagttgaat
     3601 tatgtttctg cagctgaagc cttatccctg attaaaagca atagccgtgt atttctgcat
     3661 ggcagcgcat gtactcctgt ttatctattg gaagagttgg ccaaaaatgc cggtaactat
     3721 aagaatgtag aactggtttt catttccctt caaggtcctg agatggagat tgccaagccg
     3781 gaatatgcag attcattcca tgtcaacagt ctttttgtca gtgctccggt acgtggggct
     3841 gtcaatagcg gttttgctga ctttgttcct gtcttcctga gtgatattcc ggatttgttt
     3901 aatgatggaa tcttcccgat tgatgttgcc atcatacatg tgacaccacc ggatgatcat
     3961 ggatattgct ccatgggagt atctgtcgat atagcacgtg ctgcggtcaa taatgcaaaa
     4021 aaaatcattg ctctggtcaa tccgcgaatg cccaggacaa tgggtgacgg gatgatccat
     4081 tataagcata tcgataagat ggtctttcat ccttctgaac tacctgaggt agattatagt
     4141 tccaaggtga cagaaatcga ccgtatgata ggcaggaata tcgctgagat ggtggaggat
     4201 ggatccacac ttcagttggg aattggtact atcccggact ccgttctgac ctaccttggc
     4261 aaccataaag atcttggtgt acatacagag atgcttagtg acggtatcat cgatctcttc
     4321 ctcaataacg tcatcaataa ccgcaagaag cgaattcacc caaatcgtat ggtgacaagc
     4381 tttgccctag gaacacgacg cctctatgac tttgtggata ataacgccgg attagctttt
     4441 ctcgatatcg attatgtcaa taatccttct gtcattgcaa aaaatgacaa ggtggttgcc
     4501 atcaactcat gcattgagat agatcttacc ggccaggttt gctccgatag catcggcact
     4561 taccaatata gtgggatcgg aggacaaatg gacttcatcc gcggcgcagc tctcagtaaa
     4621 ggcggtaaac caatcattgc catcaacagc cgaaccagga aaggacagcc acgtatagtc
     4681 ccgacactca aacccggtgc aggtgtggta acttcccgcg ggcatgtaag atatgtggta
     4741 actgagtacg gagtcgtcaa ccttttcggc ctcaatatgc gccaacgtgc gaaagcattg
     4801 atagaaatcg cacacccgga tgatcgggaa atgttgcatc agagcgctta tgaacgtttc
     4861 aagatttttc tttaagtaag gaatctactc ttaagtgcca atttttccct gttctgccgg
     4921 cctattacga ttaattttcg tcaaattgat tttacaaccg atgtcaagaa gttgatttct
     4981 attttttcat cttct
//
LOCUS       DAOQQN010000243         4966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7758_length_4966_cov_5.892894, whole genome shotgun sequence.
ACCESSION   DAOQQN010000243 DAOQQN010000000
VERSION     DAOQQN010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7758_length_4966_cov_5.892894"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PLG24_09300"
     CDS             <1..1137
                     /locus_tag="PLG24_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25129.1"
                     /translation="ESNLFPPFGLLLKAQLRDMFEDYRLEGGVRIPVALNGNEAYLVY
                     DDLKYRLDKRVALYRKASNERLNIGNISTQVRKNIILMGQYDVKYALDQFNAVKVGLT
                     LRQDRSFYKDTDLNSLDKPNETDQRVGIRVTYILDNTLDKGLNLMAGTRIRVFGEWVK
                     QFEISFDKRFNFNFNKGHMWVGGFDARNYMELGRHSILATRVAGQVSFGTERILYYLG
                     GSDGWLFPGFNNETQTASDVNFAYQVPAPSLRGFQYNIRNGNNYGLISNELRVPFLRY
                     LSRRPISSTFLNNLQLVGFFDVGSAWTGWNPFTKYNPLNTVYISQPGIDVKVNYFRNP
                     IVFGYGAGIRFLLFGYFIRLDYARGWDTGNLTPPRMYISLGTDF"
     gene            1560..2000
                     /locus_tag="PLG24_09305"
     CDS             1560..2000
                     /locus_tag="PLG24_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HMZ25130.1"
                     /translation="MKVLNLFAIILLITTSSFANTNENTDSGVSTKENYIHTMSHHGD
                     VISNPPGNITTTFKVAGNCGMCKKRIEAAAKSVKGVKAANWDGITEMITITYNEQKTD
                     LMEVHKKIAAAGYDTELVQATDESYNSLHGCCHYDRLQYDKKEE"
     gene            complement(2642..3616)
                     /gene="pfkA"
                     /locus_tag="PLG24_09310"
     CDS             complement(2642..3616)
                     /gene="pfkA"
                     /locus_tag="PLG24_09310"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HMZ25131.1"
                     /translation="MIKRVGVLTSGGDSPGMNAAIRATVRTAIYYDLHIYGFMNGYEG
                     LIDGSIKRLEKGDVANIIQRGGTILKTSRSARFMTKEGRQMAYETLLANDIDALVTIG
                     GNGTLAGAALFAEEFNIPIIGIPGTIDNDLSGTDYTIGFDSAINTAIEAVDKIRDTAD
                     SHNRLFFVEVMGRHSGYIAMFTGIGSGAGNVLVPEVDTNIDDLVKTLKLAQKRDKLFS
                     IIIVAEGSQLGSAHDVANKVLEHIKDVDAKVTVIGHLQRGGSPSCQDRVLSSRLGYAA
                     IYGLMHGESGVMAGVINNKVIFTPIKEALAQPKVFTDDFIKLANILAI"
     gene            complement(3635..4471)
                     /locus_tag="PLG24_09315"
     CDS             complement(3635..4471)
                     /locus_tag="PLG24_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25132.1"
                     /translation="MRIIVDLGGTKGDWCFILRDGRMESYVTRGFNPVLQSMWDLEQI
                     IKSELPHLTEPVEVMHYYGAGVHGETIYNSLYTTLKHLMHTDEVEIHNDLMAACRATC
                     QHESGIVSILGTGSASCLFNGTDIEDKIPSLGFIAGDEGAGSRIGKRVLQAYFYREMP
                     TDIQAEYEENYPLGREEILENIYKRPGANSYLASFANFALTQRRHPFMENIISEEFDR
                     FVVRHLLKYNNARQLKCHFVGSIAWFAQDILKASLERHNLTLGSVIRKPIDDLVRYHS
                     HS"
BASE COUNT         1321 a         1138 c         1051 g         1456 t
ORIGIN      
        1 gagtctaatc tcttcccgcc attcggcttg cttctgaagg cacaactgcg ggatatgttt
       61 gaagattaca gactggaagg aggtgtaagg attcctgttg ccctcaatgg caatgaagct
      121 tatctggttt atgacgacct gaaatacaga ctggacaaaa gggtggcatt ataccgtaag
      181 gcctccaacg aaagactaaa catcggcaat atttccacac aggtcagaaa gaacatcatc
      241 ctgatgggac aatatgatgt caaatatgcc cttgaccagt tcaatgctgt caaagtgggc
      301 ttgacacttc ggcaagacag gtctttctat aaggatacag acctcaattc gctcgataaa
      361 cccaatgaga cagatcagcg ggtgggcatc agggttacct acatcctgga caatacgctg
      421 gataaggggt tgaacctaat ggccggaaca aggatacggg tctttggaga gtgggtcaag
      481 cagtttgaga tcagcttcga caagaggttt aatttcaatt tcaataaagg ccatatgtgg
      541 gttgggggtt ttgatgcccg caattatatg gagttgggcc gtcattctat cctggcgacc
      601 cgggtagccg gacaggtgtc ttttggcact gaaaggattc tctattatct cggtggaagt
      661 gatggctggc tttttccggg tttcaacaac gagacccaga ctgccagcga cgtcaatttc
      721 gcctatcagg tccctgcacc aagcttgcgc gggtttcagt acaatattcg caatggtaac
      781 aattacggac tgatcagtaa cgaacttcgt gttcctttcc tccgatacct tagtcgacgc
      841 cccatcagct ctaccttttt gaacaatcta caattggtgg gcttcttcga tgtcggttca
      901 gcgtggacag ggtggaatcc atttaccaag tataacccac tcaatacagt atatatttca
      961 cagccaggca tcgatgtcaa ggtcaattat ttcaggaatc ccatcgtttt tggctatggt
     1021 gccggaatcc gcttcctgct gttcggctac ttcatcaggt tagattacgc ccggggctgg
     1081 gacacaggaa atctgacgcc tcctcgaatg tatatcagcc tgggcacgga tttttaaacc
     1141 tgctgaatcg taactattca caacctatca gcctggccac atcgataagc tatagaaaat
     1201 ggagaactta atatcttgca ctcatagaag cgtcattaaa aattatacta ccttgatttg
     1261 ttggcgtttt tttagcttgg attatgcacc attatcacat aagacatgaa ttataatttg
     1321 tttataacca tccaactatt cttccggata gaaaataagt atatttggtg atatcttcga
     1381 taaatattgg ttcctgaaag tctacttcag atcctattat tctgcgctga aagcttggtc
     1441 taacttgaat ttaatggcaa ttaaacgatt ccctgttctt ttttgttaac tttgtattgg
     1501 ttggatagta acatgtatca caagaccgtt tctttatttt ttatttaaat aattatgtca
     1561 tgaaggtatt gaatcttttt gcaattattc tattgattac aacttcatcc tttgctaata
     1621 ccaatgaaaa cactgattcc ggagtatcaa ccaaggaaaa ttacatacat acaatgtcgc
     1681 atcatgggga tgtcatttca aatcctccgg gcaatatcac caccacattc aaggtggccg
     1741 gaaattgcgg catgtgtaaa aaacggattg aagctgctgc caaatccgtt aaaggggtta
     1801 aggctgccaa ttgggatggc attactgaaa tgatcactat cacatacaat gagcagaaaa
     1861 ccgacttgat ggaggtacat aagaagatag ctgctgcagg ttatgacacc gaactggtac
     1921 aggcaaccga tgaatcctac aactcattgc atggatgctg tcattatgat cggctgcaat
     1981 atgacaaaaa agaggaatga tgtgcctgtg aaaaaacatt tgattaccgc aatggatgtc
     2041 tataggggtc tgcccgctca tttttgcgaa atctgaaagc acggtgtatg catctatatt
     2101 tttttgatgg tttgtcttca aaataatttt gctactccgg aatggagtcg catattatag
     2161 ctacaggctt caaccatgtc atctgttttc gaactgaaac tttatcatgt cgggactata
     2221 ctcgggcatt ttgtgtagta cttccggttt ttttcacccc tggacccgct catgaccaaa
     2281 tggctattcc ggtcctttac ctcctgagtg cgtctgtatt tgtttgtcat gaccaacctg
     2341 aatgtatgct ttgatccata ataaccatgg ttataaaatg gcttctggat ggctttcact
     2401 acccgggtgt ggtgtcttcg ggttattgtt gtagaaaatt ccattacctc atagcatcaa
     2461 gctgctattg gtcaaaaaaa tagctttgaa aatatttaaa ttatgctttc aggcaacctt
     2521 tgtaaatatc acaatgcaat aaaggccccg ttcacagatt tgacatcaga ttctaatcct
     2581 acattcagta gttgacacat ctcacaccaa ccgctacaac cttcattgaa gtatctttta
     2641 attatatcgc cagaatattc gccagcttaa taaagtcatc ggtaaatacc ttgggttgtg
     2701 ccaacgcttc cttgataggc gtgaaaataa ccttgttgtt gataactcct gccataacac
     2761 ccgactcgcc atgcatcaat ccatagatgg cagcataccc aagtcgactg ctcaggacac
     2821 ggtcctggca tgatggagat ccgcctcgct ggagatggcc gataacggta accttggcat
     2881 cgacatcctt gatatgttca agtactttgt tcgctacatc atgagcactg cccaactgac
     2941 tgccttctgc aactatgatg atagaaaaca atttgtcccg cttctgtgcc agtttgagtg
     3001 tctttaccag atcatcgatg ttggtatcta cttctggaac caatacgttg ccggctccgc
     3061 tgccgatacc ggtaaacatg gcgatataac cggaatgtcg tcccatcact tctacaaaaa
     3121 agagcctgtt gtgtgagtct gccgtatcgc gtattttatc gacggcctca atggccgtgt
     3181 tgatggcgga gtcgaagcca atggtataat ccgtacctga caagtcattg tcaatcgtcc
     3241 ccggaattcc aatgatgggg atgttaaact cttcggcgaa aagggctgcc cccgcaagag
     3301 tcccgttacc accaatggta accagggcat cgatgtcatt ggccaacagg gtctcataag
     3361 ccatttgcct gccctccttc gtcatgaatc tcgcactgcg ggaagttttc aggatggtac
     3421 cgccacgttg aatgatgttg gcaacgtcgc ccttttccaa tcgcttgatg ctgccatcaa
     3481 tgaggccttc ataaccattc atgaagccat agatatgcag gtcgtaatag atggctgtcc
     3541 tcaccgttgc acggatggcc gcattcatgc ccggtgaatc tccgcctgat gtcaacactc
     3601 cgactctttt gatcatgggt ttgcttttta aatgttatga gtgactgtgg tatcttacaa
     3661 gatcatcaat cggtttgcgg atgacactgc ccagggtcag attatggcgt tccagtgagg
     3721 ccttcaggat atcctgggcg aaccatgcga tactgcctac aaaatgacat tttaattgcc
     3781 gggcattgtt gtatttaagc aaatgccgca ccacaaaacg atcaaattct tcagaaatga
     3841 tattttccat aaatggatgc cggcgctgtg tcaatgcaaa attggcaaat gaagcaagat
     3901 aactgttggc ccccggccgc ttatatatat tttccaggat ttcttctctc cccagtgggt
     3961 agttttcttc atattctgcc tggatatctg tcggcatctc ccggtagaaa taagcctgca
     4021 atacgcgctt gccaatacgc gaaccggcac cttcgtcccc tgcaataaaa cccaatgacg
     4081 gaatcttgtc ttcgatatcc gttccattga agagacagga cgcggatcct gtacctaata
     4141 tcgatacaat gccgctttca tgttgacagg tagccctgca tgctgccatg agatcgttgt
     4201 ggatctcaac ctcatcggta tgcatcaggt gctttagcgt agtgtacagg gaattgtaaa
     4261 tggtctctcc atgtactccg gcaccatagt agtgcatgac ttccaccggt tcggtaagat
     4321 gcggcaactc ggatttgatg atctgctcga ggtcccacat gctctgcaag acaggattaa
     4381 atcctcgcgt gacataactt tccatccgtc catccctgag gatgaagcac cagtcgcctt
     4441 ttgtgccacc cagatcaact atgattctca atgccgctaa ttttaacgtg caaaattagt
     4501 gtttttctta cactaagcaa agattattga caaattcata gtgaaggcaa tagcaaactt
     4561 ctttccatct attagcattt agtccttcct gactcggaat atccctgaat ttctatccat
     4621 tattccggat tgaccttttc cacttatagt aggtatgact tgatgatgaa tgatttttgg
     4681 gtaagtttga tatgtacgta caataaatcc tttacttcct aaagtattat ggtcccattc
     4741 gcgtcaaatt ggcgctattt cccccggacg ctttaagctc ccggagcttt aatgcctgac
     4801 taatactgat gttttacaga gccgctctgc cggccgcgtt agcttctgca aatgccgcta
     4861 cccggttatg catacactaa ttgactacca aaatattccc acaattgggt tgaatcacga
     4921 ctaagccgaa taactcgaat tttattgtgc tttacaaata tagctt
//
LOCUS       DAOQQN010000244         4942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7807_length_4942_cov_5.385308, whole genome shotgun sequence.
ACCESSION   DAOQQN010000244 DAOQQN010000000
VERSION     DAOQQN010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7807_length_4942_cov_5.385308"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..964
                     /locus_tag="PLG24_09320"
     CDS             122..964
                     /locus_tag="PLG24_09320"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016079909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HMZ25133.1"
                     /translation="MKNIPWETVKEYQDITFRYHNGVARLAFNRPEVRNAFRPETVSE
                     LLEALIICHESQEIGVVLISGEGPSPKDGGWAFCSGGDQRVRSKTGYKGGDGINRFNI
                     LDVQRQIRFMNKVVIAVVPGWAVGGGHSLHVVCDLTLASKEHAIFKQTDVDVASIDGG
                     FGSAYLARQIGQKRAREIFFLGKSYSAQEAFEMGMVNAVIAHEELEYTAYEWAQEILA
                     KSPTAIKMAKFALNLIDDGLVGQQVYAGEATRLIYMTEEAEEGRNAFLEKRKPGFDKF
                     PKFP"
     gene            976..1887
                     /gene="menA"
                     /locus_tag="PLG24_09325"
     CDS             976..1887
                     /gene="menA"
                     /locus_tag="PLG24_09325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HMZ25134.1"
                     /translation="MANISSWLTAFRLHTLPLALSTIIMGSALAYDRGSYNGMVTLWA
                     CLTTVFLQILSNLANDYGDTASGVDNADRIGPKRGLQSGDITKGQMKKAIYSFIALSL
                     ISGILLIMNGTRELRITYGLILFIIGIGAIAAAMKYTMGKNPYGYSGYGDIFVFLFFG
                     LVGVLGTYFLHTHNLVSIIVLPAIAMGCLSAGVLNLNNLRDRDNDAASGKHTLVVKLG
                     LKNAKRYHAILLITAMLAMIAFSILSGGGLKYWLYLPSFIGIIRSIVVVFRNEDPVAL
                     YPELKNLALNTLSLSLMVGVGALLTGM"
     gene            complement(2117..3355)
                     /locus_tag="PLG24_09330"
     CDS             complement(2117..3355)
                     /locus_tag="PLG24_09330"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HMZ25135.1"
                     /translation="MVDDTLIWQSKVRNLEKNLYYKDIQVSYLSQITREINENASSER
                     LFHRFAGFLSTEMAVDRMLLLFRDDDGWKIRSSIGFDELPEQDFEPILSSYNSPGFLL
                     KKDKKMLPGMEYIIPVKHKDTAIAYALIGHFGSELRTHDNYEFVATLANIIAVAIENK
                     RLFNRQLEQERFKNEMKLATEVQQMMLPGKWPECTSFEVSSIYRPLWNIGGDYLDCIK
                     IDKNRTAFCIADVSGKGIAAAMLMANFQAVLHQVIKSSVGLEDIVRELNRAVIRIANT
                     EKIVTFFIGEYNSKTKTLRYINAGHVPPVLYMDSEILFLDQGTTLLGAVEELPDLRPG
                     TLHIHSDAMIVLYTDGITDLKDPGGRSFEDEILFDFVRKNADRKVREFNDGLLQILEQ
                     FNDADKFPDDIAVLTCKICA"
     gene            complement(3359..4558)
                     /locus_tag="PLG24_09335"
     CDS             complement(3359..4558)
                     /locus_tag="PLG24_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ25136.1"
                     /translation="MVQRPGFLRQFAREWPRTIALVFILVCTLMAFFAYLITSDKTPD
                     VNRQISGIGLKKPGFTVTLLAFPKPGISDNPSFLHFLLNGKEDDHRYLAVSNYKIDGD
                     SLKYSLYEEDAHSGDLPVTTIHKVKWLLGDEVIKIDKSNQLYSITLEDGRHIELSENE
                     VDSRLQQSGIIKRTYLLGTDLFGRSLLDRIILGIRYSLFIGFLAVLLSITIGTVIGML
                     SGYFGGKTDDVLSFLMNVAWSIPTLILVFAIVLVTGRGIQNIFIAVGLTMWVDAARLV
                     RGQVLSLKKEKYVEVARTMGMSTPRILFRHILPNMLGPVIVIAVSNFASAIIVESGLS
                     YLGFGIQPPTPSLGSILSENYGFILTGKPMLAIIPVVILLLLVLSFNLVGNGVRDIFD
                     VKSNLQP"
BASE COUNT         1406 a         1078 c         1089 g         1369 t
ORIGIN      
        1 actaaaaagg ttatttgatg gaaatacagg tagaactatc ctggaaatat ttactgatgc
       61 tgacaaaaat ccgttagttt tgggtcaata ttggaattat ctgaaagaaa agaattgaac
      121 gatgaaaaat ataccctggg aaacagtcaa agaatatcag gacatcacat tccggtatca
      181 caacggcgtc gccaggctcg cattcaatcg gccggaggtg cggaatgctt ttagaccgga
      241 gactgtatcc gaattgttgg aagcgcttat catttgccat gaatcacagg aaattggcgt
      301 cgtactgata agtggtgaag ggccttcgcc caaggatgga ggctgggctt tctgttccgg
      361 tggtgaccaa cgggtacgtt caaagactgg atataaggga ggtgatggaa tcaatcggtt
      421 caatatcctg gacgtacagc ggcagatcag gtttatgaac aaggtagtga ttgccgtagt
      481 gcccggatgg gcagtgggtg gtggccacag tcttcacgtg gtatgtgacc tgaccttagc
      541 gagtaaggaa catgcgatat ttaaacagac ggatgtggat gtagccagta ttgacggggg
      601 gttcggatca gcttatctgg cccgtcagat agggcaaaaa cgagctcggg agatattctt
      661 cctcggcaaa tcctattccg cgcaagaggc ttttgagatg ggcatggtca atgccgttat
      721 cgctcatgaa gagctcgaat atacagccta tgaatgggca caggaaatac ttgccaagag
      781 ccctacagct atcaagatgg ccaaatttgc ccttaatctg atcgatgacg ggctggtagg
      841 ccagcaggta tatgccggag aggccactcg attgatctac atgaccgaag aggcagaaga
      901 aggccgaaat gcttttctgg aaaagagaaa acctggattc gataagttcc ctaaattccc
      961 ctgattccgg ttgtcatggc aaatatctct tcctggctta ctgccttccg gctgcatacc
     1021 ctgccattgg ccttatccac catcattatg ggcagcgcct tagcgtatga tcgaggcagt
     1081 tataatggaa tggtgaccct ttgggcgtgt ctgacgactg tctttttgca gatactgagc
     1141 aaccttgcca acgactatgg agatacagca agtggtgtgg acaatgcgga tcggatcggg
     1201 ccaaagagag gtctgcaatc gggagacatc accaaaggcc agatgaagaa ggctatttat
     1261 tctttcattg cactttccct tatatcaggc attcttttga ttatgaacgg gaccagggaa
     1321 ctacgtataa cctatggtct gattctcttc atcataggta tcggtgccat tgcggcagcc
     1381 atgaaataca ctatgggtaa gaatccttat ggctattcag gttatggtga tatctttgtt
     1441 ttcttgtttt ttggtctggt tggtgtgctg ggcacttact ttctgcacac ccacaatttg
     1501 gtgtcgatta tcgtccttcc tgccatagcg atgggctgcc tcagcgccgg agtgcttaat
     1561 ctgaacaatc tccgggaccg tgacaatgat gccgcctccg ggaagcatac gcttgtggtc
     1621 aaactgggac tcaaaaatgc caagcgctac cacgccattc ttcttatcac agccatgtta
     1681 gccatgattg ctttcagcat tctgtcgggt ggtggcctga agtactggct ctatctacct
     1741 tcatttatcg gcatcattag aagtatcgtt gttgtcttcc gcaatgaaga ccctgtagcc
     1801 ttgtatcctg agcttaaaaa tcttgccctc aataccttgt cacttagttt aatggttggc
     1861 gtgggagctt tattaaccgg aatgtgatcg tttgttgaat ctaagattca taaaaaatga
     1921 aaataaaaaa tttacagcgt attcccagag gcaatcaaat tattgaaccc tcctaatccc
     1981 ggtatcaggt aaaagataat aaggggcgca aaaaatcctg tcaaagaaaa cctcatcacc
     2041 gactggtgaa gtagtgtaca atgtttttac aacaagattt tttcaacaaa ttaaaagatg
     2101 attcaataac cctgtctcaa gcacaaattt tgcaagtcag aacggcaata tcatcgggga
     2161 acttatcagc atcattaaac tgttccagga tttgtaacag gccatcattg aattccctta
     2221 cctttctatc ggcattcttt cttacaaaat caaaaaggat ctcatcttca aacgatctgc
     2281 ctcccggatc cttaagatct gttatgccgt cggtataaag gacgatcatt gcatccgaat
     2341 gaatatgcaa tgtgcccgga cgcaggtcag gtagttcttc cacggctcca agcagggttg
     2401 tcccctgatc gagaaatagt atttcgctat ccatgtataa gactggcggc acatgacctg
     2461 cattgatgta acgcagcgtt tttgtcttgc tgttatattc acctataaaa aaggtgacaa
     2521 tcttctcggt attagctatc cggataaccg ctctgttaag ctcgcgcaca atatcctcca
     2581 ggccgacact cgacttgatt acctgatgca ataccgcctg aaaattggcc atcaacatag
     2641 ctgctgctat accttttccg gaaacatctg caatacagaa agccgtacga ttcttatcaa
     2701 ttttgataca atcaagatag tcaccaccta tattccacaa cggccgataa atcgacgaaa
     2761 cttcaaaact agtgcattca ggccatttgc ccggcaacat catttgctga acttctgtag
     2821 ccagcttcat ttcattcttg aagcgttcct gttccaactg acggttgaag agtcttttgt
     2881 tttcaatcgc tacggcaatg atattagcaa gggtcgcaac aaactcataa ttgtcatgtg
     2941 tcctcaactc ggaaccaaag tgcccgatca acgcatatgc tattgcagta tccttatgct
     3001 ttaccggaat gatatattcc ataccgggga gcattttttt gtcttttttc aaaaggaagc
     3061 cgggactatt gtaggatgac agaattggtt cgaaatcctg ttccggcaat tcatcaaaac
     3121 cgatgcttga gcgaattttc catccatcat catcccgaaa caacaacaac atcctatcca
     3181 cggccatttc ggtgctcaaa aaaccggcaa agcgatggaa caagcgttct gaagaagcat
     3241 tttcattgat ctcacgagta atctgggaca gatagctgac ctgtatatcc ttgtaataaa
     3301 ggttcttttc gaggttcctt accttacttt gccagattaa agtatcgtcc accatggatc
     3361 agggttgtaa attggatttt acatcaaaga tatctcttac tccgtttcca accaagttaa
     3421 atgacaaaac cagcaacaat aaaattacta ccgggatgat agccagcatc ggcttgcctg
     3481 tcagaataaa tccgtaattt tcgctcagga tggagccgag agaaggagtg ggtggttgta
     3541 taccgaagcc cagatagctt aagccactct ccactatgat agcggaggca aagttggaaa
     3601 ccgcaataac aatgactggg ccaagcatgt ttggcaatat atgcctgaat agaatgcgtg
     3661 gtgtactcat acccatagta cgggcgactt cgacatattt ttcctttttc agggacagta
     3721 cctgaccgcg gacaagtctt gctgcatcca cccacatagt caagcctact gctatgaaga
     3781 tgttctgaat accgcgtcct gttaccagca ctatggcaaa aaccaaaatc aaggtcggta
     3841 tgctccaggc aacattcatc agaaagctga ggacatcgtc tgtcttgcct ccaaaatatc
     3901 cgctcaacat accaatgacc gtaccaattg taatggaaag caggacagca agaaatccta
     3961 tgaaaaggct ataccggatc ccgagaataa tcctgtcgag cagtgaccgc ccaaacaaat
     4021 cagtgcccag cagatatgtt cttttaatga tgccggactg ttgtagccgg ctatccactt
     4081 cgttttcgct taactcgata tgtcgaccgt cctccaaggt tatggagtat agctgattgg
     4141 atttgtcaat tttaattacc tcgtcgccca ataaccactt gaccttgtgt atcgtagtta
     4201 ctggtaagtc tccagagtgg gcatcttcct cataaaggct gtatttaaga ctatcaccat
     4261 caatcttata attggacaca gccagatagc ggtgatcgtc ctcctttcca ttcagcagga
     4321 aatgcagaaa agagggattg tcagaaatac ctggttttgg gaaggccagt agagtcactg
     4381 taaagccggg ctttttcagt ccgataccgg aaatttgtct gttgacatca ggtgttttgt
     4441 cacttgtgat gaggtatgca aaaaacgcca ttagtgtaca gaccaagatg aataccaagg
     4501 caatggtgcg cggccattcc cgtgcaaatt gtcttaagaa gccaggcctt tgtaccatat
     4561 gttattgcat ttaaatctgt aaagtaacaa ataagttaac gataattatt gaatatttta
     4621 acaaaatgaa tagtgataat atcaaggtaa ggaaatatca acatgcactt ctaaaactat
     4681 taaggtgaaa agatgtgcag ctaccaacaa ttagtaaaat aagatagaaa tggtataatc
     4741 gataagaaaa ctaattattg tttgggggtg gtgccgcctc tgaagtaggt agatgattaa
     4801 tagccataat gcttgatggt attgcctttt ggggcggctt tgccaaaatt cagatatttt
     4861 attcgggaat taaattttct atttgcctcc aaatccttgt gtatagcggt ttttgaatta
     4921 ttactttcct acctactacg at
//
LOCUS       DAOQQN010000245         4936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7820_length_4936_cov_5.183774, whole genome shotgun sequence.
ACCESSION   DAOQQN010000245 DAOQQN010000000
VERSION     DAOQQN010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7820_length_4936_cov_5.183774"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /gene="glgB"
                     /locus_tag="PLG24_09340"
     CDS             complement(<1..1369)
                     /gene="glgB"
                     /locus_tag="PLG24_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HMZ25137.1"
                     /translation="MNGGVVPFSLFTEYDIHLFRNGLHYKLYNLLGSHLVVVNGVKGV
                     YFAVWAPSASNVTVMGDFNYWRTSQYRLNPRWDNSGIWEGFVPGVKQGAIYKYNITGP
                     DGKVYEKGDPYAFRWEVPPGTGSIVWDIKDYSWRDKSWLEGRKKFNKLQSPISAYEVH
                     LGSWKKPERKETSFLTYKQLKDELVPYVKEMGFTHVELMPVMEHPYYPSWGYQIIGYY
                     ATTSRFGSPQEFMELVDAFHQEGIGVILDWVPSHFPSDGHSLSYFDGTHLYEHGNPQL
                     GYHPDWKSLIFDYGKKEVKSFLISNAVFWCEMYHADGLRVDAVASMIHLDYSRKDGEW
                     TPNKHGGRENLEAIDFIKEMNQAVHAYHPDVITIAEESTSFPFVTAPVNDGGLGFDEK
                     WMMGWMHDTLKYFALDPIHRQFHHDQITFSMVYAYSEKFVLPFSHDEVVHGKGSLLTR
                     MPGDEW"
     gene            1935..2657
                     /locus_tag="PLG24_09345"
     CDS             1935..2657
                     /locus_tag="PLG24_09345"
                     /inference="COORDINATES: protein motif:HMM:NF027590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMUG2 DNA glycosylase family protein"
                     /protein_id="HMZ25138.1"
                     /translation="MIKRHSFAAKVIDFNYNLKYDGAIPEAFKVLNPYLDNPETMIVM
                     RQFYEKYYNDNRKRKFIIGINPSRHGAGVTGVPFTDTKRLETVCGITLQSAHTHEVSS
                     VFLYDMIEQYGGADKFYGNFYINSPFPLAIVRKAKDDNFLNANYYDDSRLFDCVKEFM
                     IDSLKRHFNMGLDTDEVFVLGVKNGSFIKKLNDNHRFFKKMTILEHPRFIQQYKSKEK
                     QLFIDKYIEALKGEESATKKRE"
     gene            complement(2680..3687)
                     /gene="murB"
                     /locus_tag="PLG24_09350"
     CDS             complement(2680..3687)
                     /gene="murB"
                     /locus_tag="PLG24_09350"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HMZ25139.1"
                     /translation="MLSLKNLHTFHCDVSANDLIRLESPVKVTQLFDDGFFSTEKWML
                     LGGGSNVLFLKDFEGIILLNRMKGKEILAVNESSVLVRFSSGEVWHDIVLWSLEQGFN
                     GIENLSLIPGTMGAAPIQNIGAYGVELKDVFISLRAFDTISGEVVTLSKERCNFGYRD
                     SVFKHELKGRMIILSVDLLLRRDGITDVAYGDILKTLAARGFQAPYKPKDVSDAIIQI
                     RQYKLPDPEVLGNAGSFFKNPVIPASHYNELKKEDPDIPGYIVNDSEVKVPAGWLIER
                     CGFKGRRVGNTGSHEKQALVLVNYGNASGSEIHSLAVEIQQEVAKKFGIEIFPEVNFI
                     E"
     gene            complement(4091..4879)
                     /locus_tag="PLG24_09355"
     CDS             complement(4091..4879)
                     /locus_tag="PLG24_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HMZ25140.1"
                     /translation="MADILHKDTYNIHLPVFDGPFDLLLFFIERDELDIQNIPIAKIT
                     GDFLDYIRSMEEINIDLAGEFIVTAATLMRIKARMLLPMKKNEETQELEDPRDELVNK
                     ILIFKSFKEVSEELSRLETERAMLHTRGGILSDIKRIQHASVSYNELESANLYNLIRT
                     FNRLLARMNDPDKSNVHQIVDYPYTIQDCREDILNAVRNNEKCSFDTLFSGCENRIHA
                     IVQFLSLLELINQQLVKIVNASGANAFWISEKKTEERDSSDEAN"
BASE COUNT         1576 a         1039 c          861 g         1460 t
ORIGIN      
        1 gccattcatc acccggcata cgggtcaata gacttccctt gccatgaact acctcatcgt
       61 gcgaaaatgg caggacaaac ttttcagagt aggcatatac catactgaaa gtaatctgat
      121 catggtgaaa ctgacgatgg ataggatcaa gtgcaaaata cttaagagta tcatgcatcc
      181 agcccatcat ccacttttca tcaaaaccga gaccaccatc attgaccggt gcagtaacaa
      241 agggaaacga agtcgattct tcggcaatcg taattacatc cggatggtag gcgtgtactg
      301 cctgattcat ctccttgatg aagtcaatcg cttcaagatt ctcacgtcca ccatgcttat
      361 tgggtgtcca ttctccatcc tttctggaat aatcaaggtg aatcattgaa gcgacagcat
      421 caaccctcaa accatctgcg tggtacattt cacaccaaaa aacagcatta ctaatcaaaa
      481 aggatttaac ctctttcttt ccataatcga atatcaggct cttccaatca ggatgatagc
      541 ctaactgagg attgccatgc tcatacaagt gagtgccgtc aaaataactc agtgaatgtc
      601 cgtcactcgg aaaatggctg ggtacccagt caaggataac accaataccc tcctgatgaa
      661 aggcatcgac cagttccata aactcttgcg gacttccaaa tcgtgaggtt gtagcataat
      721 acccgattat ctggtatccc caggaaggat agtatggatg ctccattacc ggcatcaatt
      781 caacatgtgt gaagcccatt tccttgacat agggcacgag ttcatctttc agttgtttat
      841 aggtaagaaa actggtttcc tttctttccg gcttcttcca gcttcctaaa tgaacttcgt
      901 atgcagagat cggagattgc agtttattaa acttttttct cccttcgagc catgatttgt
      961 ccctccagga atagtctttt atatcccaga caatactgcc ggtgccggga ggaacttccc
     1021 aacggaaagc atagggatcg cccttttcat agaccttacc atcaggtccg gtaatgttgt
     1081 atttgtaaat tgcaccttgc tttactccgg ggacaaaccc ctcccagata ccgctattat
     1141 cccaacgtgg attaagcctg tactgtgaag tacgccaata attgaaatcg cccattacag
     1201 taacatttga cgcagaaggc gcccatacag caaaataaac gccctttacg ccattaacaa
     1261 caacaagatg gctccccagt aagttataca atttataatg gagcccattc ctgaataaat
     1321 gaatatcata ttcagtaaaa agagaaaagg gtacaacacc accattcata gcaacacggt
     1381 tttattgata aatgtaagaa taaaatgtca attggtatca aaatcacttc taaacaaagt
     1441 gttgacattg gaatttcgaa aaaaatgcat cctatagctt ttggatcgac taaactgact
     1501 atgcaaatcc ttcataacct gttctttcga atgtacgtgg gcaaaaattt ttgagctatc
     1561 aggagaacgg tcctggtaat attcattaaa atatatgcaa aaagtctaac tttcgtactt
     1621 gagtagtaag gaaatgcact gagaagttcg taataaaccg atttcctgaa aataccatta
     1681 aatttatctt attaataaac tatgctatta cccaaattaa agtatccgtc aaagaggaga
     1741 ctaattcacc ggctcaaaat ccttcacata aaaactaatg gtcaaattat tgaatatgag
     1801 caaaagtgat aatttaataa ccttttcaaa atgaatttaa atactattaa aagaaggttg
     1861 tttgttttga aggttgtttt aaaacctaac gcatttcaaa ctgttcctat aagcaaaaat
     1921 gtaatcccga aacgatgatt aaaagacatt cttttgcggc caaagtaatt gactttaatt
     1981 ataacctgaa gtatgatgga gctattccgg aagcatttaa agttttgaat ccatacttag
     2041 acaaccctga aaccatgata gtgatgcgtc agttctatga aaagtattat aatgataata
     2101 gaaaacggaa atttatcatc gggatcaatc caagtcgcca tggtgccgga gttactggcg
     2161 ttccatttac agataccaaa agattggaga cagtctgcgg cataaccttg caatcagcgc
     2221 atacacatga agtttcctca gtattcctgt atgacatgat tgaacagtat gggggggcgg
     2281 acaaatttta tggaaatttt tacattaatt ctccatttcc acttgccatt gtccgtaaag
     2341 caaaagatga caacttccta aatgccaatt attatgacga ttccaggtta tttgactgtg
     2401 taaaggaatt catgatagat tccctcaagc ggcattttaa tatgggactt gacacagacg
     2461 aggtttttgt cctgggtgtt aagaatggtt cttttatcaa gaaattgaac gataatcatc
     2521 gtttttttaa aaaaatgaca attcttgaac atccgcgatt catacaacaa tataaatcaa
     2581 aggaaaaaca gttgtttata gataaataca tagaagcttt gaaaggtgag gaaagtgcaa
     2641 ctaaaaagcg tgaataacga aatatataat actttatact tattcaataa aattgacttc
     2701 cggaaatatt tcaataccaa actttttggc aacttcttgt tgaatttcca cagccagtga
     2761 atgaatttca ctgccacttg catttccata attaaccagg accaatgctt gtttttcatg
     2821 acttcccgta tttcctactc tcctaccctt gaatccgcaa cgttctatca accaacctgc
     2881 cggaaccttt acctcactat cattaactat atagccggga atatcaggat cctccttctt
     2941 taattcatta taatgagatg cgggaatcac aggatttttg aaaaaacttc cggcattgcc
     3001 tagaacttcc ggatcaggta atttatactg cctgatttga attattgcat ccgaaacatc
     3061 ctttggtttg tagggcgcct gaaaccccct tgctgcaagc gttttcaata tatcaccata
     3121 agctacatcg gttattccat cccgacgcaa taacaaatca acggaaagga tgatcattct
     3181 acccttaagt tcgtgtttaa atacactatc cctataccca aagttacatc tttctttgga
     3241 aagggtaaca acctctcctg agatcgtatc aaatgcacgc aaagatataa atacatcctt
     3301 caactctact ccataggccc caatgttctg tataggtgct gcacccatag ttccgggtat
     3361 taaggacaga ttttcaatgc cattgaaacc ctgttccaat gaccaaagga ctatatcatg
     3421 ccacacttca ccagaggaaa accttaccag gacagaagat tcgttcactg caagaatttc
     3481 cttacccttc attctgttga gcaagattat accttcaaag tcctttaaaa aaagtacatt
     3541 gctacctcct cccagcaaca tccatttttc agtcgaaaag aagccgtcat cgaacaattg
     3601 agtgactttg acaggagatt caagcctgat caaatcattg gcagaaacat cacaatgaaa
     3661 ggtatgtagg tttttaaggg acaacatata accaaaggta acaataagtc ttcaatttat
     3721 tcaagattca tagattcgga tataacatca aaataatctt ttatgccatc ttgacatgat
     3781 tgatccttct tatatcaaac tgtaaatcat caagctacgc catcaattgg ccttattagt
     3841 ccattgttgt ctcaaccatt actacggatg tcatgcaagt aattgtttca ataatgaaca
     3901 caattccagg gatttatttt attgcgagga tcacgaagct ttcgcctcac atactgagcg
     3961 ttacctttgc tgaaaagaac ctaagaacat aagcaaaaga acaccaatct acaccaaata
     4021 aactgatttt aagaaaggac tcatatgact ggtcagtagg gttgttttca cattgtgttg
     4081 ccgaaccagg tcaattggct tcatctgacg aatccctttc ttccgttttt ttttcagaaa
     4141 tccaaaaagc atttgctcct gaagcattta ctatcttaac caattgctga ttaattaact
     4201 ctagaagcga cagaaactga acgatggcat gaattctgtt ttcacaaccg gaaaacaagg
     4261 tgtcaaaaga gcatttttca ttatttctta cagcattgag gatatcctcg cggcaatctt
     4321 gaatggtata ggggtaatct acaatctggt gtacattact cttatcaggg tcattcattc
     4381 tagccagaag ccggttaaat gttctgatca ggttgtataa gttagcagat tcaagttcat
     4441 tatagctgac agaagcatgt tgaatccgct ttatatcaga aagtataccg cctctggtat
     4501 gcagcatggc acgttcggtt tccaatctgg acagctcttc tgagacctct ttgaaacttt
     4561 tgaatatcag aattttatta accaattcat ctcttggatc ttcaagttct tgtgtttctt
     4621 catttttctt cattggcagt aacatcctgg ctttaatgcg cattaaggta gctgcagtga
     4681 ctatgaattc gccggccaga tcaatgttga tctcttccat actgcgtata taatccagga
     4741 aatcgcctgt aattttagct ataggaatat tctggatatc caattcatcc cgctcaataa
     4801 aaaacagaag caggtcaaac ggtccatcga agaccggcag gtgtatatta tatgtgtctt
     4861 tatgaagaat atctgccatg agaatgcgaa gataagagat ttggctttac ttttgaggtc
     4921 aaattatcaa attgaa
//
LOCUS       DAOQQN010000246         4895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7914_length_4895_cov_5.321488, whole genome shotgun sequence.
ACCESSION   DAOQQN010000246 DAOQQN010000000
VERSION     DAOQQN010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7914_length_4895_cov_5.321488"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..660
                     /locus_tag="PLG24_09360"
     CDS             67..660
                     /locus_tag="PLG24_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HMZ25141.1"
                     /translation="MNRISLPADIVVQEKSLLSDNWYHLYRYRFTYTTATGRKINQTR
                     EVYDRGNGASVLLYNEEKNTVILVRQMRFPTWLNGNKEGYLLEAIAGLLDEDDPKECA
                     CRESVEETGYQPINLSKVCSVYVSPGAVTEILHCYVAEYNDILRQGTGGGIDDENIEV
                     LEMDFCDVWNMLLKGEIRDAKTVILLQHLKLSGKMKS"
     gene            complement(976..1713)
                     /gene="mtgA"
                     /locus_tag="PLG24_09365"
     CDS             complement(976..1713)
                     /gene="mtgA"
                     /locus_tag="PLG24_09365"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HMZ25142.1"
                     /translation="MKVKELIGKVRRIHYKRWVYTIVFTFLGLSLVSVVTLRFIPVPV
                     TPLMVIRSVEQLKDKERDFRWEKDWVSLDHISKNAVKAVLASEDQAFFDHNGFDFVAI
                     EKAIAHNKVSTKKIGASTISQQTAKNVFLWQGRNWVRKGLEAYFTVLIELVWPKERIL
                     EAYLNVIEFGDGIYGIEAASKHYFHKSASELNKKEAAMLAAVLPNPLKYKVNAPSDYL
                     SKRCRRIMNQMNTIDYIQSQAQYTLAK"
     gene            complement(2088..2447)
                     /gene="rplS"
                     /locus_tag="PLG24_09370"
     CDS             complement(2088..2447)
                     /gene="rplS"
                     /locus_tag="PLG24_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HMZ25143.1"
                     /translation="MDAIQFVNEQTLSVNNNMDFGPGDHIEVSYKIIEGAKERTQLFK
                     GDVIQIKGHGLTKSFTVRKISNGVGVERIFPFASPSISGIKIVKRGRVRRARLFYLRD
                     RVGKAARIKEKTSYAKS"
     gene            complement(2566..4797)
                     /locus_tag="PLG24_09375"
     CDS             complement(2566..4797)
                     /locus_tag="PLG24_09375"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ25144.1"
                     /translation="MRRVLFFIFTVIFSSLIITSVQAKHIIGGYISYEFISSTNNINK
                     YQITLRVYRDCGNPQNGPFDRPAMLSIATSDGKYYRRDFNMGNPVITNLSNLFSNPCI
                     TKPPQVCVQEGVYTQTVDLPVDPNRSYYIIYQRCCRNETIFNILNPGEYGATYYTEIT
                     PLAQQLQNSSPYFKTIPPIAICAGFNVTFDHSAEDIDGDSLVYHMVTPLNGGGQGGFG
                     APNCQQVDPQPDCLPPYDSVYYRPPFKGQNPMGGSPTVAIDYKTGLLTGTPNIVGQFV
                     VGVRIDEYRNGVLLSSSIRDFQFNVANCEKVVRAELSVETRKGKDIQFKFCGDQLLKI
                     ENISSLESSIFNYSWEVNYPFGKDTIFSTKDLDLAAFDYGNYFGKMILNKDLQCTDTA
                     TFEFTKFPGLHADYTFSYDTCKDEGVRFVSQSYGDTNLPLTLSWADKDGAFGSTTEID
                     HGFPGSGDYPVRLTITDTNGCVDSVETVIPYYPIPDKVLQWKQIDDCVPAQIVFPSLH
                     PAIDNSYNIEWDFGDGTTGNGLAPMHEYTRVADYDISVHVTDKVGCEYSGLFRRGVRI
                     HDLPTAGFDYSPKELSNIQPVMKISDDSKDAVKWIYYIGNLDSLTEQHPSYTFRDTGL
                     ITIYQVVANQFGCEDTISTLVDVVPINTIYMPNAFLPASSGENSEFRPAGNGFGIVEY
                     EMTVFDRFGEIVFRTDEFNQGWSGKNKNGDYLPNGAYGVKVRLLGPRGELKKLSGKAM
                     LIR"
BASE COUNT         1462 a         1075 c          928 g         1430 t
ORIGIN      
        1 actggtgaaa atgcctgttc tattgatggt acctgcgaat aaattatcgc aataaaataa
       61 agacagttga acaggatttc attaccagca gatatagttg tccaagaaaa gtcattactt
      121 tccgacaatt ggtatcattt gtacagatat cgattcacct atacaaccgc taccgggcgt
      181 aaaatcaatc agacaaggga ggtctatgac cgtggtaatg gagcttctgt gttattgtac
      241 aacgaggaaa aaaacacggt catccttgtt cgtcagatgc gcttccctac ctggctcaat
      301 ggcaataaag aaggatattt attagaagcc atcgcaggct tgttggatga agatgatcca
      361 aaagaatgtg cctgtaggga gtcggtagaa gaaacaggct atcaaccgat caatctttcc
      421 aaagtatgtt ccgtgtatgt atcgccgggt gcagtcaccg aaattctcca ctgctatgta
      481 gcagaatata atgatattct acgccaggga acaggaggag ggatagatga cgagaatata
      541 gaagtgttgg aaatggattt ctgtgatgtc tggaatatgc ttcttaaggg agagatccgt
      601 gacgcaaaga cggttatttt gctgcagcat ctgaaattgt ccggcaagat gaaaagctaa
      661 gacagttggt ttattttccc atccccgtat ttcacagaaa aggcttgact acaatcaata
      721 ttatctacaa aagatagaaa agcataaaat ttcctctaat gccttttatt ggcttcgaat
      781 tccccgtcta ttccgaaccg gctaacgaat ataattcact gatcaactaa aaaaccggaa
      841 aagtgatata actcagcgga ctgacctcac tatgaaattc attctgtcaa ttcaaattcc
      901 taaaagtatt ccatcattca atagaggacc cttacaatga tgtgatgcaa tttcccaaag
      961 gcctcaaaag gcatcttatt ttgcaagtgt gtattgtgcc tgggattgaa tgtagtcgat
     1021 ggtgttcatc tggttcataa tccttcgaca tcttttggat agatagtcag agggagcatt
     1081 gaccttgtat ttaagcggat tgggcaggac agctgccagc attgccgcct ctttcttatt
     1141 tagttcagag gcacttttat ggaaataatg tttggaggca gcctcaatcc cgtatatccc
     1201 atccccgaat tcgattacat tgagataagc ttcaagaatt ctttccttag gccacaccag
     1261 ttcgatcaat acggtaaaat atgcttccag cccttttctc acccagtttc ttccctgcca
     1321 gagaaaaacg ttcttggctg tttgctggga aatggtactg gctccgattt ttttggtgct
     1381 gaccttattg tgagcaatag ccttttctat cgccacaaag tcaaatccat tgtgatcaaa
     1441 aaaagcttga tcctcacttg ccagaaccgc cttaacggca tttttagaaa tgtgatccag
     1501 cgatacccaa tccttttccc acctgaagtc ccgttcctta tctttcaatt gttcaacact
     1561 tctaatgacc atcaacggtg tcaccggaac aggtatgaat ctaagtgtca ccacagatac
     1621 caaactcaat ccgagaaaag tgaacacaat agtatagacc caccttttat agtgtatccg
     1681 tctaactttc ccgatcaatt cttttacttt catatgtcaa tgggactgca attttaatgc
     1741 cattccaaac atattataat attttttaat atattgtttg agttaattta ggatcttagt
     1801 catgattcaa agctgagaca tcaaaatatt gacatacttt tcacaaaaaa ttgacacttt
     1861 caggtaccaa tttttattat acaacatttc gaaagaattg tcctgtaatt tcaccccttt
     1921 gagtatggcc gcattagctg tatgcacaat ttggaaatat aactcagatc aaagcctgtt
     1981 tgctatagtt ctcataaaaa acaattccgg gcaaaatata attcaaaaaa gcataaaaaa
     2041 aagctgtccc cgggggaaca gcttttataa ctttatttac ctcagtttta acttttggcg
     2101 taagaagttt tttccttaat acgggcagct ttacctactc tgtctcgcag gtaaaacaac
     2161 cgtgcacgcc tcactctacc tcttttgaca atcttaattc ctgaaatgct gggtgaagca
     2221 aaggggaaga tcctttccac tccgacccca ttggaaattt tccttactgt aaaactctta
     2281 gtaaggccat gtcctttaat ctgaataaca tctcccttga agagctgcgt tctttccttt
     2341 gcaccttcta tgatcttgta gcttacttcg atgtgatcac cgggcccaaa atccatatta
     2401 ttgttgacac tgagagtctg ttcatttaca aattgtattg catccatgac gctaaaattt
     2461 ttatatcctt tcttgaaatg aggcacaaag gtaaataatt caattgaatt aacctacaat
     2521 ggccctaaaa atttgttatt tttttatatt ggctctttac ctgaatcatc tgatcagcat
     2581 agccttgcct gacagcttct tcaactcccc tcgtggcccc agcaatctga ccttgacgcc
     2641 atatgcacca ttgggcaaat aatcaccatt tttattcttt ccactccaac cctggttgaa
     2701 ttcatcggtc cggaatacga tctctccgaa tcggtcaaac acagtcattt cgtactccac
     2761 aataccaaaa ccatttcccg ccggcctgaa ttcactgttt tcgccactcg aagccggcaa
     2821 gaaggcatta ggcatataga ttgtattaat gggcaccaca tccaccaggg tggaaatcgt
     2881 atcttcacag ccaaactgat tggccactac ctgataaatg gtaatcaatc cggtatctcg
     2941 gaaggtatag cttggatgct gctcagtcag actgtcaaga ttgcctatat aataaatcca
     3001 tttgacggca tccttactat catccgatat cttcataacc ggctggatat tggacaactc
     3061 cttgggtgag tagtcaaatc cggctgtagg aaggtcatgg atccttacac ctcgcctgaa
     3121 caatccgcta tattcacatc ccaccttatc agtcacatga acactgatat catagtcagc
     3181 aaccctggta tattcatgca tgggggcaag tccattcccg gtcgttccat ccccaaaatc
     3241 ccattctata ttatatgaat tgtcaatggc cggatgaagg gaaggaaaca caatttgagc
     3301 aggtacacaa tcatcaattt gcttccattg cagcacttta tcaggaatcg gatagtaggg
     3361 tatgacagtc tctacactgt ccacgcaacc attggtatcc gttatggtca gcctcaccgg
     3421 ataatctcct gaccccggaa agccatggtc tatctccgtt gtggaaccaa aggcgccatc
     3481 cttgtctgcc catgaaagtg tcagagggag gttggtatct ccataggatt ggcttacaaa
     3541 gcgtactcct tcatctttgc atgtatcata actaaaggta taatcagcat gtaagcccgg
     3601 aaatttggta aattcgaatg tagcggtatc cgtacattga agatccttat tgagaatcat
     3661 tttaccgaaa taattaccat aatcaaaagc agcaagatcc aagtccttgg tggaaaatat
     3721 cgtatccttg ccaaaaggat aattcacctc ccaggagtaa ttgaaaatgg acgactccag
     3781 cgaacttata ttctcaatct tcaaaagttg atctccacaa aacttaaatt ggatatcctt
     3841 accttttctt gtctccaccg aaagttcagc acgtaccact ttctcacaat tagccacatt
     3901 gaattggaaa tcccgaatgg aagaacttag taagacgcca ttgcggtact cgtcaatcct
     3961 cacaccaaca acaaactgtc cgacaatatt gggtgttccg gtcaacagcc ccgttttgta
     4021 atcaatggca acagtaggag agccccccat tggattttgc cccttaaatg gtggcctgta
     4081 ataaacacta tcataaggag gcaggcaatc aggctgcgga tcgacctgct ggcaattggg
     4141 tgcaccaaat cctccctgac cacctccatt aagaggggtg accatatgat atacaagact
     4201 atcgccatca atatcttctg cagaatgatc gaaagtaaca ttgaatcccg cacaaatggc
     4261 tattggaggt attgtcttaa aataaggact tgaattctgc agttgttgcg ccagtggtgt
     4321 aatttccgtg taataggttg cgccatattc tcccgggttg aggatattga agatggtttc
     4381 attgcgacaa catcgttgat aaataatata atagcttctg ttaggatcga ccggcagatc
     4441 gaccgtctga gtataaactc cctcctggac acatacctgg ggaggcttag tgatgcatgg
     4501 attactaaaa agattggaca aattcgtgat taccggattc cccatattga aatcacgtct
     4561 gtaatatttt ccgtccgagg tggcaatcga aagcatggcc ggcctgtcaa aaggaccatt
     4621 ctgagggttg ccgcaatccc gatacacacg tagtgtaatt tgatatttat ttatgttatt
     4681 cgtggaggat atgaattcat aactgatata tccacctata atgtgtttgg cctgaactga
     4741 ggtaataatt aatgaagaga aaataactgt aaagataaaa aaaagcactc tcctcatttt
     4801 gttaaacgaa attgaacatt gaaataattt ataaagaata attataagaa tttagccgac
     4861 aagttacgaa gtttttatac gaatgtatat caggc
//
LOCUS       DAOQQN010000247         4895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7915_length_4895_cov_4.748347, whole genome shotgun sequence.
ACCESSION   DAOQQN010000247 DAOQQN010000000
VERSION     DAOQQN010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7915_length_4895_cov_4.748347"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..718
                     /locus_tag="PLG24_09380"
     CDS             149..718
                     /locus_tag="PLG24_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HMZ25145.1"
                     /translation="MNVVSMDVFSRLMMDRIIFMGVPVNDYVANVIQAQLLFLESTDP
                     KRDIQMFINSPGGSVINGLGIYDTMQYVSPDVATICTGLAASMGAVLLAAGKKGKRTC
                     LYHSRVMIHQPLGGMEGQVSDMEISYKLIKKMQKELYDILAAHTGKDYDIIEKDCDRD
                     NWMTSAEAKEYGLVDEVLEKTNPSRLTQD"
     gene            793..2031
                     /gene="clpX"
                     /locus_tag="PLG24_09385"
     CDS             793..2031
                     /gene="clpX"
                     /locus_tag="PLG24_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HMZ25146.1"
                     /translation="MAKNKDLQRCSFCGKDKNEAIILIEGLDGHICESCVEKAYDIIE
                     QEIFDKQPSDNLQVSDFAKRTPKEIKEYLDNYVIGQEQAKKTLAVAVYNHYKRLYQHD
                     KDVEIEKSNIIFVGPTGTGKTLLAKSIARMLNVPFAIVDATVFTEAGYVGEDVESILT
                     RLLQVCDYDVKMAEKGIVYIDEIDKIARKSDNPSLTRDVSGEGVQQSLLKMLEGTDVN
                     VPPQGGRKHPEQKMIKVNTQNILFICGGSFDGLDKIIARRLNTQIIGYSHEAKEKVDQ
                     SNLYQYAMHQDLKHFGLIPELLGRLPIMTYLDPLNLDMLKSILIKPRNAIIKQFIKLF
                     EYEKVELIVTDDALDYIAQTALNYKLGARGLRSICEAILMDDMYELPSKKNVDRLVVD
                     LDYVKTKLAGSRIEANLKVA"
     gene            2031..2762
                     /gene="truA"
                     /locus_tag="PLG24_09390"
     CDS             2031..2762
                     /gene="truA"
                     /locus_tag="PLG24_09390"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HMZ25147.1"
                     /translation="MRLLAFCDFVGTNYVGWQRQPNGISIQEAIEHALSIYFRSPIAI
                     VGCGRTDSGVHGRNYGFHFDLPEIPEDAPFLFKINRILPDDIVIRSIKVMEDNFHARF
                     SAKSRRYIYRIHTSKDPFIQPYSWYLDRLTPESVHILNEAATFIASLKDFSSFAKTGS
                     NNKTTYCDMIQCEWKAHEDRLELHIAANRFLRGMVRLIVGGCANVAVGKLSLERMKEL
                     SLEGVVLPFAFSVPAQGLTFEGVEY"
     gene            3178..3654
                     /locus_tag="PLG24_09395"
     CDS             3178..3654
                     /locus_tag="PLG24_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HMZ25148.1"
                     /translation="MIVIGLVGIPTPRNYEEFKAAMLAEVNQYRLNGCNCGNVRMKPV
                     SALTWNDALAASAAEHAIDMHRQGYFDHKSRDGRNYPGRIKEKGYDWEIVAENIAYGQ
                     KNIAQVVSDWMASPGHCFNIMNPGIKQMGVAKKGGYWVQDLGSARENSLKARVNLR"
     gene            complement(4021..>4895)
                     /locus_tag="PLG24_09400"
     CDS             complement(4021..>4895)
                     /locus_tag="PLG24_09400"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HMZ25149.1"
                     /translation="ITNSTYKFVEAIKTAYSLGPETTLTYEDERFPESLKIIARLIKG
                     GLKTKIYVVELDGFDTHSNQIGKHEELLKQLAAAVDTFYKDLEGTEYEENVLSVTFSE
                     FGRRIEQNESPGTDHGAASVLLAFGKCLEGNGTLGKYPSLTETDEDGNLIFNVDFRKV
                     YNTLFTEWLCLEDAVSDQILLKDFGDPLDFGFTCQEVSSVKTPAKPGFVHSASYGLGD
                     VFVNYTIPKDVMVMVELVSMSGQPIVLERPMMKKAGSYSIPLQQARRRLMPGAYVYRI
                     RVTGADYTGKLIIN"
BASE COUNT         1511 a         1029 c         1063 g         1292 t
ORIGIN      
        1 caaagaacag tcaatgattc caaaagacga attcaaaaaa tacgcgacca aacacctgaa
       61 tatgaactcc atgcaccttg ataaatatat gcaggtgtca gtacctaata tccatggttt
      121 tacgcctgcc gtcattgaag agcggcaaat gaatgttgtg agcatggatg tgttttcacg
      181 actcatgatg gataggatca tattcatggg cgtgccggtc aatgactatg tagccaatgt
      241 catacaggct cagttgctat ttctggagag cacagatcca aagcgcgata tccagatgtt
      301 tatcaacagt cctggaggtt ctgtgatcaa cggcctgggc atatatgaca caatgcagta
      361 cgtctctccg gatgtggcaa ccatatgtac cggattagct gcatccatgg gcgctgtcct
      421 gctggctgca ggtaaaaagg gcaaaagaac atgcctgtat cacagtcgtg tgatgatcca
      481 tcagcctctt ggcggtatgg agggtcaggt ctccgacatg gaaatatcct ataagctgat
      541 caagaaaatg caaaaggaat tatatgatat tcttgcagcc cataccggaa aggactatga
      601 cattatcgaa aaagactgtg atcgtgataa ttggatgaca tccgctgaag caaaagaata
      661 tggattggtg gatgaagtgt tggaaaagac caacccctcc agactaacac aagattaata
      721 ataaataaat ctcaaccata taccggacat acaggatgcc cgcctggttc attttgtatt
      781 aaaagtaaag caatggcaaa aaacaaggat ttacaaagat gttctttctg tggaaaggat
      841 aaaaatgaag ctattatcct gattgaagga ctcgatggac acatatgcga atcctgcgtg
      901 gaaaaggcat atgatatcat tgagcaggaa atctttgaca aacaaccctc ggacaatcta
      961 caggtaagcg acttcgccaa acgtacgcct aaggaaatca aggaatatct tgacaattat
     1021 gtgataggtc aggaacaagc gaagaagaca ctggccgtag cggtgtataa tcactacaaa
     1081 aggttatacc agcacgataa ggacgtcgag atcgaaaaat ccaatatcat cttcgtaggt
     1141 ccgaccggaa ccggtaagac acttctagcc aagagcattg cacggatgct gaatgttcca
     1201 tttgctatcg tagatgctac tgtcttcaca gaggctggat acgttggtga agacgtagaa
     1261 tcgatcctga ccagactact ccaggtctgt gactatgatg taaaaatggc tgaaaaaggc
     1321 attgtctata tcgacgaaat agacaagata gcccgaaaga gcgacaatcc ttccctgacg
     1381 agggatgttt ccggagaggg agttcaacaa tcactactga agatgctgga aggtactgat
     1441 gtcaacgtac cgccacaggg aggcagaaag catcctgaac agaaaatgat caaggtcaat
     1501 acccaaaata tattgtttat ttgtggtggt tcgttcgatg gactggataa aatcatcgcc
     1561 agaagactca atactcagat catcggttac agccatgagg caaaagaaaa ggtagatcag
     1621 tccaacttgt atcaatatgc aatgcatcaa gacctgaagc attttggatt gattccggaa
     1681 ctgttgggac gcctgcctat catgacctac cttgatccac tcaatctgga catgctcaaa
     1741 tcaatcctta ttaaacccag gaatgccatc atcaaacaat ttatcaaact gtttgaatac
     1801 gagaaggtag aattgatagt aactgatgat gctcttgatt atatagcgca aacggctcta
     1861 aattataaac ttggagcaag aggattgcgg tccatctgcg aagcaatatt aatggatgat
     1921 atgtacgagc ttccctcaaa gaaaaatgtt gatcgattag tggtggattt agactatgta
     1981 aagacaaaat tagccggatc ccgtatcgaa gccaacctta aagttgcctg atgcgcctcc
     2041 tcgccttttg tgatttcgtc ggtacaaatt atgtaggatg gcagaggcaa ccaaatggga
     2101 taagcattca ggaagctatt gagcatgcat tgtccatcta tttccgttcg cctattgcca
     2161 ttgtgggatg cggcaggaca gattccgggg tccatggtcg caactatggg tttcattttg
     2221 atctccctga aataccggaa gatgctccat tcttattcaa aatcaacaga atcttgcccg
     2281 acgatatcgt cataagatcg attaaggtaa tggaggataa tttccatgcg agattctcag
     2341 ccaaatcccg tcgatacatc tatcgaatcc atacctccaa ggaccctttc atacaaccat
     2401 acagctggta cctcgaccgc cttacaccgg aatctgtgca tatcctgaat gaggcggcta
     2461 catttattgc aagtctgaag gatttttcat cctttgccaa aaccggctca aacaataaga
     2521 cgacctactg tgatatgatt caatgcgaat ggaaggcaca tgaagaccgt ctcgaactgc
     2581 acattgcagc taatcgcttc ctgagaggca tggtccgcct gattgtgggc ggttgtgcca
     2641 atgttgccgt tggcaaacta agtttagagc gtatgaagga actatctctc gaaggtgtcg
     2701 ttctgccctt tgctttttcg gtgcctgctc agggattgac atttgagggc gttgagtatt
     2761 gatttgacgc tcgcgggata cagggattat tacaggagga ataggcgtgt tgagaatgaa
     2821 gtgtaatttc cacccagtca gatagatatt atcttcaagg cctatagtcc ataatgtatg
     2881 aattttgaat tatatggcac tattctttct ctaaaattta tcagtggttg atttgatcgt
     2941 tctgcattca ctgatcttat cggcaatata ctacagtgct ttgcaatgca atctcttttc
     3001 aggtttatac attgacgtca tgataaaagg ccgtttccaa aatggcactg tacggaccag
     3061 aaagccattg cccctactga atacataatt tttagttaaa agggatattg cacatactat
     3121 ttcaagcctt tcaggagtcc taataaaaaa tagatgtatt aagtgattcc cttgttaatg
     3181 atagtaattg gattggtggg catacctaca cccaggaatt atgaagaatt caaggctgcg
     3241 atgcttgccg aggtcaatca atatagattg aatggatgca attgtgggaa tgtcagaatg
     3301 aagcctgtat cagcacttac atggaatgac gcattggccg cttcagcagc cgagcatgcg
     3361 atcgatatgc atcgacaagg atactttgat cataaaagca gggacggccg caactatcct
     3421 ggtagaatca aagaaaaggg ttatgattgg gagatagtcg ccgaaaatat tgcctacggt
     3481 cagaagaata tcgcccaggt ggtctcagac tggatggcaa gcccgggaca ttgtttcaat
     3541 attatgaatc cgggaatcaa acaaatggga gtagcaaaaa aaggaggata ttgggtacag
     3601 gacctgggtt cagccagaga aaacagtttg aaagccaggg tcaatctaag ataataaggc
     3661 aactgcaaaa aatatacaat cgactattct gccaataact atcatgacct gtacgagtta
     3721 taccaagatg atttaaataa tagtccttaa atttttagta taatgccgag gttacagtct
     3781 tatagatcaa tgaaattggt ctaaaacgaa tgaaccttcg gtattagatt cccgatctgc
     3841 tatgccctgc aatgatccat ggctgaagtc aggcaggtca tcatgaaaca ataaataaag
     3901 aacctgcgaa taaaccgaga tatccacaga tgcagacaac aaggtacagt tttcaaaatt
     3961 tcaatgtttc caatctacat cttgccgctt acaacattcg cctacttcat gagaatccta
     4021 tcagttgatt atcaacttac ccgtgtaatc cgctcctgta actctgatcc tgtacacata
     4081 agcacccggc ataagcctgc gcctggcttg ttgcaaagga atactgtagg atccggcctt
     4141 tttcatcatg ggtcgttcaa gcacgatggg ttggcctgac atagatacca attccaccat
     4201 caccatcaca tcttttggaa tagtataatt gacaaataca tcacccaggc cataggaggc
     4261 actgtggaca aatcccggtt tggccggtgt cttcacggat gaaacttcct gacaagtaaa
     4321 gccgaaatcc agcggatctc caaaatcctt tagtaaaatc tgatcagaaa cagcatcttc
     4381 cagacaaagc cattccgtaa acaaagtgtt gtagactttc ctgaaatcga cgttaaaaat
     4441 caggtttcca tcttcatcag tttcggtcag acttggatat ttgcctaggg ttccattacc
     4501 ttcaagacat tttccaaaag ccagtaatac actggcagca ccgtgatcgg taccgggact
     4561 ttcattctgc tcaatccttc ggccaaactc agaaaaggtt actgaaagaa cattctcctc
     4621 atattctgta ccttccaggt ctttgtaaaa agtatcaact gcggcggcga gttgttttaa
     4681 caattcctca tgcttaccga tctgattgga atgcgtatcg aaaccatcca gctcgacgac
     4741 ataaatcttc gttttcaaac cacctttgat caagcgcgca ataatcttga gtgattcagg
     4801 gaacctctca tcctcataag tcaaagtagt ctcagggcct aagctgtagg cggttttaat
     4861 tgcttccaca aacttgtatg tactgttggt aataa
//
LOCUS       DAOQQN010000248         4889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_7934_length_4889_cov_5.005792, whole genome shotgun sequence.
ACCESSION   DAOQQN010000248 DAOQQN010000000
VERSION     DAOQQN010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7934_length_4889_cov_5.005792"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PLG24_09405"
     CDS             <1..627
                     /locus_tag="PLG24_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HMZ25150.1"
                     /translation="ESLITHEIRDYGADRTVILDLHTTSADGGIYTIVPDHPLAIEYA
                     LGIKAPVVLGFLEGLKGTTLHYFTSHNFPGEVTSICFEAGQHDDPMSVNRAIAAITNC
                     LRYAGCVEGRHIEGRHIEVLSEYSGQLPPLTRLAYRYPINPDEGFRMLPGFQNFQPIH
                     KGQHLADNHVGPILSPMDGFILMPLYQSKGEDGFFIVKRASLYKDDEF"
     gene            complement(688..1083)
                     /locus_tag="PLG24_09410"
     CDS             complement(688..1083)
                     /locus_tag="PLG24_09410"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HMZ25151.1"
                     /translation="MKDQKQDKIDLPNRKFWNKVKKAAKGGGGSLIYAALLMYYSFRR
                     KETPTWAKAAILGSIAYFLSPVDFIPDLTPVLGYTDDLAVFVSGLITVGSHIDSEVRN
                     KAKDKLQQWFDSEEIQKAIKAIDKKMAKQ"
     gene            complement(1299..1371)
                     /locus_tag="PLG24_09415"
     tRNA            complement(1299..1371)
                     /locus_tag="PLG24_09415"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1336..1338),aa:Met,seq:cat)
     gene            complement(1433..3793)
                     /locus_tag="PLG24_09420"
     CDS             complement(1433..3793)
                     /locus_tag="PLG24_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HMZ25152.1"
                     /translation="MSLVLTSSLTAQVQEHIIYQKVKVHLNGRDLRDLARLGVEVEHG
                     YKRQEDVLINFFTEEEVKSVREAGFKVDILQEDAVAHYLEESKKVKPVDLQLAVRNPK
                     CEPDGKVYDVPQNFKYGSMGSYLTYEELISELDLMHQKFPHLITERNWFPNLKTKGGN
                     FVYYVKISDNPNDDEGDVENQMLFTALHHAREPMSMMQLVYFMWYILEKYDTDPEIRY
                     LVNKSEMIFVPCVNPDGYIYNQMTHPSGGGLWRKNRNNTNGPGGVDLNRNYSVGFAFD
                     NEGSSPIYSSDTYRGAYQLSEMETKTIHTLRLNNQFKMAINYHSYANVLIYPWGYLEQ
                     NTKDKLSYFYFSQDLTQFSDYSYGLNSETLGYPINGSADDYLYQNGILAFTFECGGPE
                     LTDEESFWPLQNKIQPICADMLYQNIRALWLLNEGIQYQLTSDKIIRPGNNTVKFLFT
                     KTGLSDDPVKISVSPLTNNIDNFNKTITLNLNHLEKQEIEIPINAHNNTTGPVALEIS
                     SIYNGSYIKRDTINLRLFNYQVLYENNGDDLSDFIKQNTDENWTLDVVDYYDTFASIS
                     VTNKTTYDKAENKILELKYPVNLPENDSLAFFSYFAKWSIEKNIDHLKTYISTDGVYW
                     DAICGNFSQAGNIDQGEEEPVLDGFQPEWVNVVYNISDYIGKTVYFKLVFRSDAKVNR
                     EGIRIDKLQILSVPGGKVSNHKTEALTIQVFPNPTSNYLYISGIGQQKAVFSLLDAQG
                     KILRKEVISNGTNQIDVSNLTSGLYIYQILNENGIVIQTGKTVIAK"
     gene            4265..4867
                     /locus_tag="PLG24_09425"
     CDS             4265..4867
                     /locus_tag="PLG24_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HMZ25153.1"
                     /translation="MKEVFIVSATRTPIGSFGGSLSGFSATDLGAASIREAYTKINLD
                     PNEINEVYYGNVVQANLGQAPARQAALKAGLSKNVVTTTVNKVCASGMKSVILAAQSI
                     MLGHNDLVVAGGMESMSNVPFYLPNMRWGSKYGSVQALDGLQKDGLLDVYNGNAMGVF
                     ADATAEKFGFSREDQDAFAIQSYKRSAEATLSGKFNAVKL"
BASE COUNT         1409 a          901 c          944 g         1635 t
ORIGIN      
        1 gaatcattga taacgcacga gatacgagat tatggtgcag acaggacggt tatccttgat
       61 ctgcacacta cttcagctga cgggggcatc tatacgatag tacctgacca tcctcttgcc
      121 atcgagtatg cattgggtat taaagctcct gtggtattgg gttttctgga aggattgaaa
      181 ggaaccactt tgcactactt tacaagtcat aatttccccg gagaggtaac aagtatttgt
      241 tttgaggcag gtcagcacga tgatcctatg tcagtcaaca gggcaattgc tgcaattacc
      301 aactgtctgc gttatgcagg gtgcgttgaa ggcaggcaca ttgaaggtcg tcatatagaa
      361 gtattgtctg aatattccgg ccaacttccg cccttaacca ggttggctta ccgttaccca
      421 attaacccgg atgaaggatt tagaatgctt ccgggcttcc aaaattttca accaatccat
      481 aaaggtcagc atctggctga taaccatgtc gggcccattc ttagtccaat ggatgggttc
      541 attctgatgc cattatacca aagtaaggga gaggatggat tttttattgt aaaacgagct
      601 tcattataca aagacgatga attttgaaag tgccgattca ttaacttaaa ccccaaatat
      661 tattactctt tttgatattc atggcaatta ttgctttgcc atttttttat cgattgcctt
      721 tattgctttc tgtatttctt ctgaatcaaa ccattgctgt aatttatctt tggctttatt
      781 acggacttct gagtctatgt gagaacctac agtaatcaaa ccggagacaa ataccgccaa
      841 gtcatcggtg taccccaata caggagttag gtcaggaata aaatcaaccg gggaaaggaa
      901 ataggcaatg gaccccaata ttgcagcttt tgcccaggta ggggtttcct ttctcctgaa
      961 agaatagtac atcaataatg ctgcataaat caatgatcct ccgccacctt ttgctgcttt
     1021 ctttacttta ttccagaatt tacggttagg gaggtcgatt ttatcttgtt tctggtcttt
     1081 cattaaatat ggtaattgac taaaatttga tttaattata tcaaattatt tttagtcatt
     1141 ttaagttcct tagacaggta attttaatta aaatttagtc tattagatct atcggaaatg
     1201 tatggagatt gcagataatc gatctacctc ctgagaaaga ggggtagggg aagattaaaa
     1261 acaaaaaaag gaaagttgat tactttcctt ttcagtagta gcggaggcag gactcgaacc
     1321 tgcggccttc gggttatgag cccgacgagc taccaactgc tccactccgc ggtatttttt
     1381 aattaggctg caaatatacg actaatatta caatttccaa ggataatatt aattattttg
     1441 caataactgt tttgcctgtt tgaatgacta tgccattttc attgagaatc tgatagatat
     1501 aaagcccgga tgttaagttg cttacatcaa tctgatttgt tccatttgag attacttcct
     1561 ttcttaatat ttttccctga gcatccaata agctgaaaac ggctttttgt tgaccgattc
     1621 cggaaatata caggtagttg ctggtaggat ttggaaatac ctggatagtt aatgcctcag
     1681 tcttgtgatt actcactttg ccaccgggaa cagaaaggat ttgcaatttg tctatacgga
     1741 taccctccct attgacttta gcatctgatc taaagacaag tttaaagtat acagtttttc
     1801 ctatataatc tgagatatta tatactacat taacccattc aggttgaaaa ccatcaagta
     1861 cgggttcttc ttccccctga tcgatattac cagcctgtga aaagttgccg catattgcat
     1921 cccaatacac gccgtcggtc gagatatatg tttttaagtg gtcaatattc ttctctatac
     1981 tccattttgc aaaatagctg aagaaagcaa gagaatcatt ttccggaaga tttaccggat
     2041 attttaattc aaggatttta ttctcggctt tatcataggt ggttttattg gtcactgata
     2101 tagatgcaaa tgtatcgtaa taatctacca catccagtgt ccagttttca tcggtattct
     2161 gtttgataaa gtccgataaa tcatccccgt tattttcata caggacttga taattaaata
     2221 gtctgagatt gatggtgtct ctctttatat aactgccatt atatatactg ctgatttcca
     2281 gcgcaaccgg accggtggtg ttattatgag cattgatagg aatttcgatt tcttgctttt
     2341 ctaaatgatt taggtttaga gtaatagttt tattgaaatt atcgatatta ttggtcaatg
     2401 gactaacact gatttttacc ggatcatcag ataatccggt tttggtaaac aagaacttga
     2461 ccgtgttgtt tccgggtcta atgattttat cacttgtcaa ttggtattgg atgccttcat
     2521 taagtaacca taatgctcga atattttgat ataacatatc tgcacatatc ggttggatct
     2581 tattttgcaa gggccagaag ctttcttcat ctgtcaattc tggtccacca cactcaaagg
     2641 taaatgccaa aataccattt tgataaaggt agtcatctgc ggaaccatta atcggatacc
     2701 ctaatgtctc gctatttagt ccataactgt aatccgaaaa ctgagtaagg tcctgtgaga
     2761 aataaaaata ggaaagttta tcttttgtgt tttgttcaag atagccccag ggatatatta
     2821 atacattagc ataggagtga tagttgatag ccattttgaa ttgattgttc aatcgaagcg
     2881 tatggatggt cttcgtttcc atttctgaca attggtaggc tcctctgtat gtatctgatg
     2941 agtaaatcgg tgaagaccct tcattgtcga atgcaaagcc tactgagtaa ttacgattaa
     3001 gatcaacacc gcccggacca ttggtattgt ttctgttttt tctccagaga ccaccaccac
     3061 ttggatgggt catttggtta tagatatacc catcaggatt aacacatggc acaaaaatca
     3121 tttctgattt attgacgaga tatctaattt caggatctgt atcatatttt tcaaggatat
     3181 accacatgaa gtacacaagc tgcatcatac tcataggctc tcgtgcatgg tgtagtgcag
     3241 taaacaacat ctggttttct acatcccctt catcatcatt ggggttatct gatattttga
     3301 cataatagac aaagttgcca cctttggtct tgaggtttgg gaaccaattt ctttcagtga
     3361 taaggtgcgg aaatttttga tgcatcaaat ccagttctga gattaattcc tcataggtga
     3421 gataggatcc catagatccg tatttgaaat tttgtggtac gtcatatact tttccatccg
     3481 gttcgcattt aggattcctt acggccagct gcaagtcaac aggtttcacc ttcttacttt
     3541 cttctaaata atgtgctact gcatcttctt gcaggatatc aaccttgaat ccggcttccc
     3601 gaaccgactt tacttcttcc tcagtgaaaa agttgataag tacatcttct tgtcttttat
     3661 atccatgctc tacttcaacc cctaagcgag ctaagtctct taaatcccgt ccattgagat
     3721 ggacttttac tttttgataa attatatgct cctgtacctg tgctgtaagt gatgatgtca
     3781 ataccaaaga caatacaagt acaatgaaag ggaatatatt acgggaaata atcatatgat
     3841 agctaaaact tcgcccaaag ataattattt tatatatatg atgattaaaa tatatttgaa
     3901 taatttttat tttttttatg ccaaatgttg atttaatgca tatttgatca ttttaattac
     3961 ataaagtgaa ttgaagcaaa taaatatttt tcacgaaacc ctgataaatg gataataaat
     4021 gggggagttg attcattgta tagagttgtt gttgttgaat tatttggtcc tccagtcatt
     4081 gatattcata ttgactagtc aggtaattcc acgcactaat attttaatca aaacaattta
     4141 ttaaaaaagt agttttctaa tttgttggta cgaagaaggg aagttttcag aattgtcttt
     4201 taaaatacct tatttttgcc gccattattc aaaattctgt ttaattatca aatttaagta
     4261 cagtatgaag gaagtattta ttgtatctgc gacacggact ccaatcggga gttttggtgg
     4321 atctctttca ggtttttcag ctacggatct tggagcagca tccattcggg aagcatatac
     4381 gaagattaat ctggatccca atgaaatcaa tgaggtatat tatggcaatg tcgttcaggc
     4441 caatctgggt caggctcctg ccagacaagc tgccttgaaa gccggactta gcaagaacgt
     4501 tgtgacaact actgtcaata aagtttgtgc gagtggcatg aaatctgtta tccttgctgc
     4561 acaatccatc atgcttggac acaatgattt ggtagttgcc ggcggaatgg agtcgatgtc
     4621 taatgtccct ttctatcttc ctaatatgag atggggcagc aagtatgggt cggttcaggc
     4681 tctcgacgga cttcagaaag atggtttact ggatgtgtat aatggaaatg caatgggtgt
     4741 atttgctgat gctactgctg aaaagtttgg atttagtcgg gaagatcagg atgcttttgc
     4801 catccaatcc tataaacgat ctgccgaagc tactctttcc ggaaaattca atgcagtaaa
     4861 attgtgataa tcatcctcaa tttcaggac
//
LOCUS       DAOQQN010000249         4854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8020_length_4854_cov_5.268806, whole genome shotgun sequence.
ACCESSION   DAOQQN010000249 DAOQQN010000000
VERSION     DAOQQN010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8020_length_4854_cov_5.268806"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PLG24_09430"
     CDS             <1..295
                     /locus_tag="PLG24_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HMZ25154.1"
                     /translation="RASRTTPFIAKAYGVPFLNIATKVMLGTHKLKDFEINPYLEGYA
                     IKEPVFSFNKFPKVDKRLGPEMKSTGEAIRFIKDLKDPYFRELEKSAAMFLYQ"
     gene            317..388
                     /locus_tag="PLG24_09435"
     tRNA            317..388
                     /locus_tag="PLG24_09435"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Arg,seq:cct)
     gene            complement(615..2252)
                     /locus_tag="PLG24_09440"
     CDS             complement(615..2252)
                     /locus_tag="PLG24_09440"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ25155.1"
                     /translation="MKRFYSKLLLVYFPCFFSCSISYAQYPKLVKDINTAGSCYPHGF
                     VRLNDKIYFQATDATHGSELWVTDGTETGTYMVKDIVPQGDSIGFQLIVFKGLLFFNG
                     DDGNSGEELWVSDGTETGTRLFLDIRASGDSYPKNFFVLNDHLYFAADDGVHGSELWI
                     SDGTVAGTHMVQDINTTGDSFCSNFFAYQNKVYFSGNDGVSGNEVWTTDGTAAGTRLL
                     ANINPASGSVPTNFAQFEDKLYFSAYTNAFGQELWVTDGTEDGTHLVKNVDGNISGYP
                     SNLTPFDGKLFFVLEDTTYGSEPWVTDGTEAGTHLFKDINPAGRSNCFNFVVYNNKLF
                     FSATDNISQNELWATDGTLGGTRLVKDIFVGGHSSPRFLTAFKDKLYFTALKSYTEGR
                     QLWSYDDNTDVLTLLTPPDADLPKPHDNDFESISLGNLLFFAASYRSNLGNELYSLNV
                     NSSSVFKSSDKEDIIRLYPNPSRNEISIQIKDYALTNATITISNSIGKIVKQIRMPEI
                     TNGFSQTFDISKWNSGIYFVNISSKELHASKMLVRIQ"
     gene            3252..3845
                     /locus_tag="PLG24_09445"
     CDS             3252..3845
                     /locus_tag="PLG24_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HMZ25156.1"
                     /translation="MVLSQDPDFFKKLSKTQTPPLLWIGCSDSRVPANEIIGAAPGEV
                     FVHRNIANMVIHSDMNMLSVLDYSVNILKVKHIIVCGHYGCGGVKAAMGNSSVGIIDN
                     WIRHIKDSYKFRKKEIEEISNEKERFDKFVEWNIKQQVLNLAVTSIVQQAWANGQELS
                     IHGWVYGLDTGLVKDLDVTFRTHDDIRNSSVYKLGFN"
     gene            4136..>4854
                     /locus_tag="PLG24_09450"
     CDS             4136..>4854
                     /locus_tag="PLG24_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25157.1"
                     /translation="MKFQYLITTLCVLAFCSTTLTGQNTNSKSNSILGKFAEKGTLTP
                     LSDVVVQLLDAVDSTLVKTELSDQLGTFSFTGLKSGKYFIKASLLGYSEYLSDPILAS
                     GDIMLPSIILSNSTVNLNEVVIQYKKPFIEREQGKVIMNVENSINAEGSSVFELLEKA
                     PGVTVSQSDNISYKGKQGIIVQIDGKDVRMGGTDLANYLRGISSSSIEKIEFIANPSS
                     KYDAAGNSIINIKIKKDKRLGT"
BASE COUNT         1559 a          935 c          851 g         1509 t
ORIGIN      
        1 cagagcaagt cgtacaacac catttattgc caaagcttat ggtgttcctt ttttgaatat
       61 cgctaccaag gttatgctcg gcacacataa attgaaggac tttgagatta atccttacct
      121 ggaaggatat gccatcaagg aaccggtctt ttcattcaat aaatttccaa aagttgataa
      181 gcgcttgggc cctgagatga aatcaaccgg agaagctatc agattcatta aagatttaaa
      241 ggatccatac ttcagggaat tagaaaaaag tgcagcgatg tttttgtatc aataaaattt
      301 atatatttgc gccctcggtc ccttagttca acggatagaa tagaagtttc ctaaacttta
      361 gatccaggtt cgattcctgg agggactact aaaagccgtc tgattcagaa aaatcaggcg
      421 gctttcttta ttccgatggt gatataccta atgaacaatt aagaacttta ttcaattatc
      481 ctactggaag tcttaaggcc ttgagaagtt acaacttaat gtttgaagat gtcaataaac
      541 ggctaaattt cagaatattt ctttaaaaat aattctacaa atcaaattaa cctcccttgt
      601 gattattatc ctctttattg tatcctgacc aacatttttg aagcatggag ttcttttgag
      661 cttatattta caaaatatat tcccgaattc catttagata tatcaaaggt ttgtgaaaat
      721 ccatttgtaa tttctggcat tcttatttgt ttgactatct tacctatgct attggaaatg
      781 gttattgtgg cattagttag cgcataatct tttatttgga tacttatttc atttcttgat
      841 ggattagggt aaagccttat tatatcctct ttgtctgatg atttaaaaac acttgaagaa
      901 ttaacattta aagaatataa ttcattacct aaattagaac gatacgatgc ggcaaagaaa
      961 agtaggttgc ccaaagagat actttcgaaa tcattgtcat gaggtttagg taaatctgca
     1021 tccgggggag taagaagtgt caatacatct gtattatcgt cataagacca taattgcctt
     1081 ccttcagtat aagattttaa agcggtaaag tataacttat ctttaaaagc tgtaagaaac
     1141 ctggggctac tatgaccacc aacgaaaata tcctttacta aacgggtccc accaagggtc
     1201 ccgtctgttg cccataactc gttctgagaa atattgtccg tagcactgaa gaataatttg
     1261 ttattgtaaa caacgaaatt aaaacaattt gaccttccgg cggggtttat atctttaaac
     1321 agatgggtcc ctgcttctgt tccatctgta acccagggct ctgatccata agtagtgtct
     1381 tctaaaacaa aaaataattt tccatcaaat ggagttaaat tagaagggta accggaaata
     1441 tttccatcga catttttaac caggtgagtt ccatcttccg ttccatctgt cacccataat
     1501 tcctgaccaa atgcatttgt atatgcagaa aaatacaatt tatcttcaaa ttgtgcaaaa
     1561 ttggtaggga cagaccctga tgcagggtta atattggcca acaacctggt cccggcagct
     1621 gtaccatctg ttgtccaaac ttcattgcca ctcactccgt cattccccga gaaataaact
     1681 ttattttggt atgcaaaaaa attactgcaa aaagaatcac cggtagtatt aatatcttgt
     1741 accatgtgtg ttcctgcaac tgtaccatca gaaatccata gttcacttcc atgaacacca
     1801 tcatctgctg caaaataaag gtgatcattc aaaacaaaga aattctttgg ataactatca
     1861 cctgacgcac gaatatctaa aaacagcctt gtcccggttt ctgttccatc ggaaacccat
     1921 agttcttctc ctgaattgcc atcatcacca ttaaaaaaca atagcccctt aaatacaatg
     1981 agttgaaatc ctatactatc cccttgtggc acaatgtcct taaccatata ggttccggtt
     2041 tccgttccat cagttaccca taattcactc ccgtgtgtgg catctgtggc ctgaaaatat
     2101 attttatcat taaggcgcac aaaaccatgt ggataacagc ttccggcagt attaatatcc
     2161 ttaactaatt taggatactg tgcataggaa attgaacaag agaaaaagca agggaaatag
     2221 acaagcaata atttagaata aaaccgtttc attgttcgaa gttttaaaaa gttaaaccat
     2281 tataagtaaa taatatatat tggataataa caaaatataa ttataatata atatatttaa
     2341 aatattatta taaaatatta tgttgccaaa cagtcatttc tgtcaataat aacacatatt
     2401 atttgggcgc cagcaggaat agcttaaaat tcaatgttta tttacactaa aataaataaa
     2461 accttgctaa tttacggatt atatataaag tctaagtaca tataatccta aataatttaa
     2521 aatttttttc atttactgga tgccttaagt taaaagtatc tgcaatttgt atgaaatctt
     2581 cattacaatt gctataaatt ctatttttta ggcaatacca tatggataac ttccttcagg
     2641 cctttgtaaa atctcattgt aattagcaca acttatatta tcttgtggta aaagtcacgc
     2701 gagctaaaaa cgtacctaat tattcttatt ggcctgtgct gaccgacatt atttgtcaat
     2761 aacaaacttg tgtgtttaat ttgcgaatat agcgaaggaa attcaggagc cttttattca
     2821 cccgggtaag aatattatcc tggcactttt gttcatcttt tttgctgtca ccttctggtt
     2881 tttactgagg agtaccaact atcataatat tggctagatt gtatttttta ttcctatagc
     2941 aattattggt cggtatttct tgtgtactgc catcatgctg atcgaatcag gaggtaaatg
     3001 gaattgattc aaggggaaga cctcagtatt tccgcctaac cgcgcattga ccacaatttg
     3061 ccttaaaacc ctgttattaa atgtaatacc gaaggtgcat tcgttataga ccaatttcat
     3121 tgatctatac gactgtaacc tcaacatccc ggaaccccgt attgtttcac tcccgggaca
     3181 gagcagagct ctagtagcat agctatccgg acaagtatac caaaaattta tggactatta
     3241 tataaatcat cttggtatta tcacaggatc ccgatttttt caaaaaatta tcaaaaaccc
     3301 agactccgcc attattgtgg atcggatgct cggacagccg ggttccggcc aatgaaatca
     3361 ttggtgctgc ccccggggaa gtatttgttc accgaaatat tgccaatatg gttattcatt
     3421 cggatatgaa catgctcagc gtccttgact attccgtcaa tatccttaaa gttaaacaca
     3481 tcatcgtgtg cggtcattat ggatgtggcg gcgtcaaggc tgccatggga aacagttctg
     3541 tcgggatcat cgacaactgg atacgtcata ttaaggacag ctataaattc cgtaaaaagg
     3601 aaattgaaga aataagcaac gagaaagaac ggtttgacaa attcgttgaa tggaatatca
     3661 agcagcaggt tttaaacctt gccgtaactt caatcgttca acaggcatgg gctaatggcc
     3721 aggaattatc aatccatggc tgggtgtacg gcctcgatac cggtctggtc aaggatcttg
     3781 atgtgacttt ccggacacat gacgacatac gtaatagcag tgtctataaa ctggggttca
     3841 attaataggt taaggctgtc cggctctttc gggcagcctt tttttatctt aaagttcgcg
     3901 gatgcaccaa atgtgtcaac atccaatctt aaagtcaaca ctgtgagtcc ggctattccc
     3961 tttatttgac atacaacgtt tttcgaacct cccctcgcct accatccatc ttttattttt
     4021 aaatctttgt cgatgagaaa attgatttaa tataagattt taattgatat atcaataaat
     4081 aaaagagagt cattattttt gtcatataat tgatatatca aataaattaa aagatatgaa
     4141 attccaatat ttaatcacaa ccttatgcgt cctggcattt tgtagtacaa ctttgaccgg
     4201 acagaacact aactcaaaaa gtaatagtat cctgggtaaa tttgcagaga agggaacact
     4261 aactcccctc agcgatgtag ttgttcaact gctggatgca gtagattcta cgctggttaa
     4321 aacagagcta tctgatcagc ttggcacttt cagtttcaca ggattgaaat cagggaaata
     4381 tttcatcaag gcatcactgt taggatattc agagtatttg agtgatccta tccttgcatc
     4441 cggggacatc atgctgccct ccatcattct cagtaacagc accgtaaatc tgaatgaagt
     4501 cgtcatccaa tacaaaaaac catttattga acgtgagcag ggcaaggtca tcatgaatgt
     4561 cgaaaacagc atcaatgctg aagggtcatc cgtatttgaa ttacttgaaa aggctcccgg
     4621 tgtaactgtc tcacagtctg acaacatttc gtataaagga aagcaaggaa ttatcgtcca
     4681 gatagacggc aaggatgtca gaatgggcgg aacggactta gctaactatc tcaggggcat
     4741 ttcatcgtca tccattgaaa aaattgaatt tatcgccaat ccttcctcca aatacgatgc
     4801 tgccggcaac agtattataa atattaaaat taaaaaagat aagcgactag gcac
//
LOCUS       DAOQQN010000250         4835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8079_length_4835_cov_5.358159, whole genome shotgun sequence.
ACCESSION   DAOQQN010000250 DAOQQN010000000
VERSION     DAOQQN010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8079_length_4835_cov_5.358159"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PLG24_09455"
     CDS             complement(<1..501)
                     /locus_tag="PLG24_09455"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMZ25158.1"
                     /translation="MRKIIFTCIFLLPFFMSFGQRTITGRVLDDAGKEGIIGANIMLL
                     KGSGTDLSTYVVTQKGTVTDIDGNFSLEAGPEENAFSVSFIGYETQTILIGQELHFEV
                     FLKEKSSLLSEVVVVGYGVQRKVDVTGSTSSVKGEDLARQPVLTATQALQGKAAGVQV
                     ISSGEPG"
     gene            983..1774
                     /locus_tag="PLG24_09460"
     CDS             983..1774
                     /locus_tag="PLG24_09460"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ25159.1"
                     /translation="MRESDRDFNAVFEKKEDQAKFERYRIKGNRIRFCTKGSDTLPAV
                     LFIHGAPASISFWKPFFRDSNLLSTARVIAADRPGYGYSGFGKLYLSYYTQAALLAPI
                     IKKYGANRKLILVGSSYGGPLCALLAAQFPQYVSGLLLVSSSIAPGEEAIWQITKIIN
                     SPLGEFFPRVLQVATAEKIHHKKMLKEIEQEWTKVVCPVIMLHGSEDELIFPANANFV
                     YSRFKSARKKISILGGLKHNIVVTKPDYIVRRIKELLDDGYLAHQ"
     gene            1849..2694
                     /locus_tag="PLG24_09465"
     CDS             1849..2694
                     /locus_tag="PLG24_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25160.1"
                     /translation="MEFIPLVITMRVLTISILLLFSTLLKAQNGVSLFHNARTTALGN
                     SDITSHNVLSGSINPANMIIGHGLFASLGIQRSFLIADINQGSLDIGYRMKNDQAIGL
                     QFYVYGNNIYSETMASLAYAIRIADRTALGLRMHGLRFSAPENKPEYAVTFTLGAQTV
                     ISEQFSLGMTTFNPVGFFRKERPNDLTSNFRIGLAYSPAPSVSLYTSANLDDAHPLNV
                     AVGIAYSIKKKVNLYLSFMTQPAVIGVGIGVPFGRGFSIDFAGAQHLQLGLSPALNLN
                     YLEVE"
     gene            2983..3189
                     /locus_tag="PLG24_09470"
     CDS             2983..3189
                     /locus_tag="PLG24_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25161.1"
                     /translation="MPRSNRKSHSRGGSFKRNVTYTQSNPEVKLHKEQLAEDARDQKK
                     FFMLLGGLTLLILIIVYLLYFRNF"
     gene            complement(3394..4497)
                     /locus_tag="PLG24_09475"
     CDS             complement(3394..4497)
                     /locus_tag="PLG24_09475"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HMZ25162.1"
                     /translation="MRKNVLFYLMAFAITMIVGCAAISKNGFTVSGEIKDAANMAVSL
                     DRIGGNNQIVPVGKANIDGQGKFKITSEEALKPGVYRLQIGERIAPIIVDGKENDIKI
                     KGALASFDDLSFEITGAKGTSEFQSAIKKLLDKTLDDAGAKTAVESATNPYAAMQIAM
                     IYFKKPTDAPIHTAVLEKLKKLDPTSDFISGYQSMINQMANPQPQGGGPISIGQMAPD
                     ISLPNPDGKNIALSSLKGKIVLLDFWASWCGPCRMANPHVVEIYNKYKDKGFTVYSVS
                     LDGVDDRARASLSPEQLQGQLAATKEKWTKAIEQDKLTWTSHVSDLKKWSSSAAQLYG
                     VNSIPRTFLIGRDGKIVAVNPRNTLEEELLKVL"
BASE COUNT         1400 a         1086 c          871 g         1478 t
ORIGIN      
        1 acccggctca ccgctgctga ttacctgaac acctgcagcc ttaccttgca atgcctgtgt
       61 ggcagtcaga acaggttgcc tggccagatc ttcccctttg acactggatg tggaaccggt
      121 gacatccacc ttacgctgta cgccataacc tacaacaaca acctcagaaa gaagactcga
      181 cttctccttc aggaagactt caaaatgaag ctcctgacct atcagaatgg tctgggtctc
      241 atagcctata aaggaaactg aaaaagcatt ctcttctggt ccggcttcta gggaaaaatt
      301 tccatcaatg tctgttactg tacccttttg agtaactaca tatgtgctca ggtctgtccc
      361 tgagcccttt aaaagcatta tgtttgctcc tatgatacct tcctttccgg catcatccag
      421 cacccgacca gttatggtcc tctgcccgaa tgacataaag aaaggaagta aaaaaataca
      481 ggtaaatatt atttttctca ctcaactgct attttattgg ttattgataa ttgaatgcta
      541 aatgaatttt aagaatgatt acgaaaatcc catacgattt gcaatgccat tggattcctt
      601 ctacgacccc tgatgccaca ttaatacatg caaagttggc aaaaaaataa attaacaggt
      661 ctctttttgc agaatattaa ccatgaaaac aaaataccaa ttaatatatt aaaattcttt
      721 gcgtttataa tattatattt gcccaggatt tgcatacaaa aaacattaga tctacctcat
      781 tttattggga taggcaacaa aaataattgc attttaatag ccggttttag atattaactc
      841 cggttggaat tgcatcagat tgactataat acttagttta aagcgtttat aatcaaacaa
      901 acattagttc atgttgagaa ttttttattt gataggaatt gttgtttttt gctcgggatg
      961 ttccatctct aattatttca agatgagaga atccgaccgt gactttaatg ccgtttttga
     1021 aaaaaaagag gatcaggcaa aatttgaaag atacagaatc aagggaaacc gaataagatt
     1081 ttgcacgaaa ggcagtgata cactaccagc cgtactgttc attcatggtg caccggcgtc
     1141 catttctttt tggaaaccat tcttccggga cagcaacctt ctttccacgg cccgggtgat
     1201 agccgcagac aggccgggtt atggctattc aggctttggt aagctctatc tatcctacta
     1261 cactcaagct gcccttttgg caccaattat taaaaagtac ggagcaaaca gaaaactgat
     1321 tcttgtaggc tccagctatg gaggaccttt gtgcgccttg ctggcagctc agtttcctca
     1381 atatgtttca ggcctgcttt tagtatcatc atccatcgcc ccgggagaag aagcaatttg
     1441 gcaaatcacc aaaataatca attctccgtt gggtgaattt tttccgcgtg tgctgcaggt
     1501 cgccactgct gaaaaaattc accacaaaaa aatgttgaag gagattgagc aagaatggac
     1561 taaggtcgta tgtccggtta tcatgctgca tggctcagag gatgaattaa tatttccggc
     1621 caatgcaaat tttgtttaca gcagattcaa aagtgcacgt aaaaagatat ccattctggg
     1681 cggtctcaaa cacaatatcg tcgtaaccaa acctgattac atcgttcgcc gcatcaaaga
     1741 attgctggat gatggctatt tggcacacca gtaatgcttc agggttcatt cattttcatc
     1801 tatcctgtgc cataaaaaac ttgtgagaaa aaattcaacc aaatagtctt ggaatttata
     1861 cctttggtaa ttaccatgag agtcctaacg atatcgatat tactcctttt ttcaacttta
     1921 ctgaaggcac aaaacggagt cagtctgttc cacaatgcga gaacaactgc gcttggtaac
     1981 tcagatatca cctcccacaa tgtcctttcc ggcagcatca atcccgccaa tatgatcatt
     2041 ggtcatggct tgtttgcatc gcttggtatt caacgctcct ttctgattgc agatattaat
     2101 caaggtagtc tggatatagg ctaccgcatg aaaaacgacc aggcaatcgg cctgcagttc
     2161 tatgtatatg gaaataacat atactcagag actatggcat ccctggcata tgccatccgg
     2221 atagccgaca gaaccgccct cggacttaga atgcacggac tgcgtttttc cgcacctgaa
     2281 aataaacctg aatatgcggt gacatttacc ttaggtgccc agaccgtcat ctctgaacaa
     2341 ttcagtctcg gaatgacaac cttcaacccg gtaggttttt tccgcaaaga aaggccaaat
     2401 gaccttacat cgaattttag aattggcctg gcatattcac cggcaccgtc tgtatcactt
     2461 tatacatccg ctaatctgga cgacgcccat cccctcaatg tcgctgtggg gattgcctac
     2521 agcatcaaaa aaaaggtcaa cttgtatctg tcattcatga ctcaaccggc tgttattggc
     2581 gtcgggatcg gagttccatt cggccggggc ttttccatcg atttcgccgg ggcacaacac
     2641 cttcaattgg gccttagccc tgctctcaac ctcaattatt tggaggtaga atagcgggat
     2701 taccccattg acatctttta ccggtcaaaa aagtatactg cagataaata atatttataa
     2761 tatcgttatt tatatatctt tgatattaaa tattttatac tttctattca tatttttata
     2821 gcaatcccaa aactgtttgg cctataagcc atgcatcccg ccaaatcacg ctacttatga
     2881 ccccatttta caaagtttaa tcaaaattat tttttataaa taattgaaag ttcatcggaa
     2941 ttttcctaaa cttgcttttt tttataacaa attaaattca atatgccacg ttcaaataga
     3001 aaatctcatt cacgaggcgg ttcatttaaa cgaaatgtaa cttataccca aagcaacccg
     3061 gaagttaagt tacataaaga gcaactggca gaagatgcaa gggaccagaa aaaattcttc
     3121 atgcttctgg gaggattgac tttattgata ctcataattg tgtatctgct ctatttccgt
     3181 aatttttaag attgcacagg tttctccttg taagaaatac cattcctgct gtttgacata
     3241 ggaatggtat ttttttttac ccgtaaatca aattcatacc taatctggtc aatggtaggc
     3301 ttgctgtaat ttaaatactt gattaatacc cggactaaaa ataaaaaacc gccggacgat
     3361 accatccggc ggttttatgt tttaaagaag tacttacaga accttcaata attcttcttc
     3421 cagtgtattt cttgggttta cggctacaat cttgccatca cgcccaatga ggaaggtcct
     3481 tgggattgaa ttaacgccat ataactgagc ggctgaactg ctccatttct tcaggtcact
     3541 cacatgactg gtccaggtca acttatcttg ttctatagcc ttggtccatt tttccttggt
     3601 tgctgcaagc tgtccctgca attgttccgg agataacgaa gctcttgccc ggtcgtccac
     3661 cccgtccaga gagacactat aaaccgtgaa tcccttatcc ttatacttgt tatagatttc
     3721 taccacatga ggattggcca tcctgcacgg accacaccaa ctggcccaaa aatcaagtaa
     3781 tacaatcttt cctttgagtg aagaaagagc aatattctta ccatccgggt tgggtaggct
     3841 gatatccggt gccatctggc cgatactgat tggcccgcca ccctgcggtt gaggattggc
     3901 catttgattg atcatggatt gatatccgct gataaaatca gacgtcggat ccaatttctt
     3961 caatttttca agtacagctg tatgaatcgg agcatctgta ggctttttga aataaatcat
     4021 cgctatctgc atggctgcat atggattagt tgcagactca actgctgtct ttgcccctgc
     4081 atcatccaaa gtcttgtcca gcaatttctt tattgcactc tgaaattcag atgtaccttt
     4141 tgctccggtg atttcaaaac taagatcatc aaaggatgcc aatgcccctt tgatcttgat
     4201 atcattctct tttccatcaa caatgatagg agcaatcctc tctccaatct gtaatctata
     4261 tacacccggc ttcaaagcct cttccgaagt aattttaaat ttgccctggc cgtcaatatt
     4321 ggctttaccc accggaacaa tctgattatt gccaccaata cgatccaatg aaacggccat
     4381 atttgctgca tccttgattt cacctgaaac ggtaaatcca tttttgctta ttgcggcaca
     4441 tcctacaatc atggtgatcg caaaggccat caggtaaaat aatacgtttt tacgcatctt
     4501 tgttggtttt tgattattga attaagacaa tccgggaaaa ctccctcctt gtatgctgaa
     4561 ttttgttttc aaacaacgaa attaaagcta ttttgttttc caagcttata ttttcccggc
     4621 taattgctat taaaagattt gttaatatgt acaaatccag tttgccgaca aacctgcagt
     4681 gtgttcaggc aggtcatcaa aaaaataata tgacctatct acaattgaaa taaccatttt
     4741 gcaatgtttc aaatttactt gattccaagc ctcccctgat gcgattttat ctcaaatcct
     4801 tataccctga tccgacttta gcaattcctt tcatt
//
LOCUS       DAOQQN010000251         4826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8098_length_4826_cov_6.538671, whole genome shotgun sequence.
ACCESSION   DAOQQN010000251 DAOQQN010000000
VERSION     DAOQQN010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8098_length_4826_cov_6.538671"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1012..1377)
                     /locus_tag="PLG24_09480"
     CDS             complement(1012..1377)
                     /locus_tag="PLG24_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25163.1"
                     /translation="MKYLTILFLFFCVLTSCNTSNQETSTNNSIKTEVVQTGNDKSKL
                     IPLSDKKVEQFAISLDSLLDVAMKAKQENNDELVKEIKSQIEALSTERENMSKSLDEK
                     ERQTFLGFTEKRINTINNL"
     gene            2837..3235
                     /locus_tag="PLG24_09485"
     CDS             2837..3235
                     /locus_tag="PLG24_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25164.1"
                     /translation="MKNKKMNGFWGLVVLFLVAFVGFSTTVNAQYVGKQLAIERIKKD
                     INSEGGSKVGGSGIETKKVPASLSPEDYSTLLRKTYLQTLLRQVVEYGNVATAIEVNN
                     NDWVQKTTGNPKRSASLPLLKNYVINLLKS"
     gene            3340..>4826
                     /locus_tag="PLG24_09490"
     CDS             3340..>4826
                     /locus_tag="PLG24_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HMZ25165.1"
                     /translation="MKRFNGAISSFVLLMFLCLCGTQGYAQTIIFNNPGSVAIPDNAY
                     NGTLASMANLPLSVSGVPNNGTAVVTISVRIGHTWAGDLTAKLVAPDGKVLALMSRPG
                     YIESADDGNGCCGSLSDFLIANTITFDDAASTPSENIGASNPIPTGSWQPSAGAISVP
                     PAYATFSALVTDLGVNNINGTWTLYIGDSGGGDTGTFSNFGMTIEFTPGGGGGTTDVC
                     ELICPADQYVTLQAGECSYYFTYPLSITPQCVVTPAPVLNFTGAFAPGNGQYPVAAGI
                     FIGSFTGNTTLTIPSTDLGSNAGTTYYQPWIWKNLPTTGTVKFNWSWTTTDGPFWDNS
                     GYLKDVPTTMTWFGNQTGTAYGCYQLTNNGGANAQSGTNVSVSVNQGGNFGFYSYTAD
                     GIGGACVLTITNFSFQAAPQLPTVTQTAGVNVGYVADFGNGYVDVITEEFPIGTTTVT
                     YSGNGPDGPIECSFNVVVNGYPNPITSLGCNDLVHVSLDPENCEAV"
BASE COUNT         1453 a          791 c          998 g         1584 t
ORIGIN      
        1 atttaaaatc ggtttttggg acggctcccg cttcattcga agcgggagct tttttatttt
       61 ggttatttgg ggccgggtat gctatttatc caatattcag taattatgat tggcttcgtt
      121 tttcttagaa tcactgtcat tgattttgga tgtatccgat tggtcacgta atttgaatgg
      181 ttttaattaa ttgactttcg attatctcct caacggttct ggcgcccttg attcaaatta
      241 ataaagccta agtattatcc cggttgggat tcggcttatt gatttttaac caataagttt
      301 taattgaaat tatcaaattg actttcacaa taattccggg atattataaa gacttggatg
      361 acaattggca accattcatc taaaatttga tgatagcctg gattgaaata ctaaaatcag
      421 agcaaatcaa tttttgtcgg aatgaccata ctttggtgct tattgtggca ttcacttgaa
      481 ggtaggtgtc aattcggaag taaaggacat tcttgcaaaa tatagatgag cctccgatca
      541 agcccacgtt ttactttccc ggatattgtt ggaactctgt acagggaggt aaggtaaatt
      601 gaacgattaa agagttacgg atatggccag tgtttggcga atataaatct agtcgcaggg
      661 atgggtggag ttcagtttgc ttgaattgaa ttcaatattg aaggatgtct gttaaaaata
      721 ataaagaatc gcaatgcacc attgatttgc taattgcgat aaatgtataa tgaaaaaaat
      781 gccatggaat caaacctgta cttttcttag aatgattagg gtgcttaatt cgaatggctt
      841 aagggggctg aattttccgt aatataggca gcctaaattt acaaaatttt taagatggca
      901 ttgaggcgtt gatttgacag gaattaaaca atagctgcaa tgtttaaata ttagatccaa
      961 atagcaaagc agcttaaggc tttgaaaaag ttgattatcc ttacaaaccg attaaagatt
     1021 gttgatggta ttgattcttt tttcggtaaa accaagaaat gtttgacgtt ccttttcatc
     1081 cagtgatttg ctcatgtttt ctctctcggt ggaaagagcc tcaatctgac ttttaatttc
     1141 tttgaccaat tcatcattat tttcttgctt tgccttcatt gctacatcca ataatgaatc
     1201 caggcttatt gcaaattgtt ctaccttttt gtccgacaga ggaattaatt tggatttgtc
     1261 attgcctgtt tggacaactt ctgttttgat gctgttattt gttgatgttt cttgatttga
     1321 agtattacaa gatgttaaaa cacagaagaa taagaaaaga atagttaaat atttcataaa
     1381 aaatcctatt tagaaaataa ttaaaaattg aaagtaaact tactgaaaac cgcaccttca
     1441 ggaacaatca tttgatggag caaaaataga taaataaaat gtatttattg taagaagctt
     1501 atggctgtta gttttggatt aatttatatt aaattggaaa taggtttgtg ttaagggata
     1561 tcaggtatta acagcaaaag cttgtcgtac aataaatccg ataaatttgt taactgtttc
     1621 cgagtttaat cccggaaatt aatatgtcaa gtgtaaataa taaaatgtta tttgtaattt
     1681 atagaaatac aaattaatac gaatacacga ttatttatat atatttaaaa tatgaattta
     1741 ctttggttgc tcctatttct gcttgaatgc ccttttgtca aatcaccccg gggttttcac
     1801 agctctcaat atgagatgca cactttacat atcttgaatt gaaccttagg accttcagag
     1861 aaattatccc tgatagttga ttttggttag aaaagacttc cacttcacgc caacatccac
     1921 aaaatgcaaa taagaagact caattattac aaaggcactc ccttgtgtgt aagaagaata
     1981 cccaccaagt ggaacttctg aaccattctg taataaagct ccttttatat catttgacag
     2041 tgatgcaaat gaataataat tacttattga atggccagct aaaatttatt tgaatatatg
     2101 ggattgtcag taacaggcat gtaatcccaa tccggacatg atgctattgt tcatttaatt
     2161 tatttagaat atttgctggt tgatcggtaa atgattctat tacctgagca gtcagggaaa
     2221 gatattcaag ctaactttat ctattgcatt ttcttttcta tcgtgcctat gggttttcat
     2281 ttttctttga tgctgtaata ttttgaaatc tgaatgatgt attcaaaaga aaagttgtat
     2341 aaattttttt cggaatgttc ctcccacacc gtattggaca aattaaaact gccaattggc
     2401 taacaaagca acaagaatga atttggagtc ctttaaaggt tcatatcaag aagcccgatg
     2461 caatttttgt tttttttttg cccattgata tcaaagtggg atgacaactc taatcgccgg
     2521 agtataatta ttaaaaaaaa atgaatattg ttttgcataa tctttaaatt aatatacctt
     2581 tgtaggctgg tattacacca ggaagacact cacattaggg aaagctattc tgaaagtcag
     2641 gtttagcctg gaattcaatc gttataagtc ttaagataat cgggcaaatg tccgggggct
     2701 ttttataaca caattatttc aatattatac taaggttctt ttttgggttc aggccaaagg
     2761 ggttccattt ttatatgtaa acaaaagaaa acgatattct aatttattaa cattttttaa
     2821 accaaaacta aagcaaatga aaaacaaaaa aatgaatggc ttttggggat tagttgtgtt
     2881 gtttttggtt gcctttgttg gtttttcaac tactgtaaat gcgcagtatg taggaaagca
     2941 attagcaata gagcgaatca agaaagacat taattccgaa ggaggttcaa aagttggagg
     3001 tagtggaatc gaaaccaaga aggtgccggc tagtttgtca cctgaggact attcgactct
     3061 gctccgaaaa acctacctcc agacactgct taggcaggtt gtagaatatg gtaatgtagc
     3121 tactgcaatc gaagtgaaca ataacgattg ggtgcagaaa actaccggta atcctaagag
     3181 atctgcttca ttgcctttac tgaagaacta tgtgattaat ttactaaaat cgtaacgatt
     3241 ggttgggcca acagagagag tagttgctct ctggaagttt gagactagat tttaaattaa
     3301 attttttatg aatcaaaatt ttaacagaat ctcatgacaa tgaaaagatt taatggggcg
     3361 ataagcagtt tcgtattgct tatgttcttg tgtttatgcg gcacacaggg atatgcccaa
     3421 acaattatat ttaataatcc cggttcggta gcaattccag ataatgccta taatggaaca
     3481 ttagcttcca tggctaacct tcctttgagt gtaagcggtg tgccgaacaa tggaacggct
     3541 gtggtgacta tttcggtgag aattggtcat acctgggcag gagacctgac tgcaaagctg
     3601 gtggctcctg atggaaaggt attggctctg atgagtaggc caggctatat cgagtcagct
     3661 gacgatggta atggttgttg cggatcttta tctgatttcc taattgcaaa tacgattaca
     3721 tttgatgatg ctgcttctac tcccagtgag aatataggag catccaatcc tatacctacc
     3781 ggatcctggc agccttctgc aggcgcaata tcagttccac ctgcatatgc tacattttct
     3841 gcccttgtta ctgatctggg ggttaacaac attaatggaa cctggacact ttatataggt
     3901 gattcaggag gtggagatac cggtactttt tccaacttcg ggatgactat tgaattcacc
     3961 cctggtggtg gaggtggaac taccgatgta tgtgagttaa tctgtcctgc ggaccagtat
     4021 gtaacgttgc aggctggaga gtgttcgtat tactttacgt atccattgag tatcactccg
     4081 cagtgtgttg taacacctgc gccggtgctt aatttcaccg gagcgtttgc gcccggcaat
     4141 ggtcaatatc cggttgcagc ggggatcttt attggaagtt ttacaggtaa tacgactttg
     4201 acaattccta gtactgatct tggatccaat gcgggtacga cgtattatca gccgtggatt
     4261 tggaagaacc tacctacaac gggtactgtt aagtttaatt ggtcatggac tacaaccgat
     4321 gggccattct gggataattc aggttatttg aaggatgttc cgacgactat gacatggttt
     4381 ggcaatcaga cgggaacggc ttacggctgt tatcaattga ctaacaatgg cggagctaat
     4441 gcacaaagtg gtacaaacgt cagtgttagt gtgaatcagg gtggcaactt tggtttttat
     4501 tcatatacag ctgatggtat tggaggagca tgtgtcttga cgattactaa cttctctttc
     4561 caggctgcgc cacaattgcc tactgtaaca cagacagcag gggtgaatgt aggctatgtg
     4621 gctgattttg gcaatggtta cgtagatgta attacagaag agtttcctat tggtacaacg
     4681 actgttacat attcaggcaa tggtcctgac ggaccgattg aatgttcatt caatgtagtg
     4741 gttaatggtt atccaaaccc gattacatca ttgggatgta atgatctggt acacgtatct
     4801 ctggatcctg agaactgtga ggcagt
//
LOCUS       DAOQQN010000252         4811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8156_length_4811_cov_4.242851, whole genome shotgun sequence.
ACCESSION   DAOQQN010000252 DAOQQN010000000
VERSION     DAOQQN010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8156_length_4811_cov_4.242851"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PLG24_09495"
     CDS             <1..280
                     /locus_tag="PLG24_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25166.1"
                     /translation="MFKESENSKYTSDNEKAMIYLQQANLAAVKGNYREAATLVKKAD
                     ELKVTESMIKDQVKQFKQALGKSGQLNMQNRMAMSQRGGSKQRRPKMR"
     gene            322..1710
                     /locus_tag="PLG24_09500"
     CDS             322..1710
                     /locus_tag="PLG24_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25167.1"
                     /translation="MGRYTFFFFFFVASYCLSGQKVAIDSISISGLEKTREYILIREL
                     NFKKGDSLDLQYLASIFQTNEKRLLNTNLVVEARCNLINLNIEAQSAGVNIHCKEALY
                     IYPAPLLEFIDNDFNKWWRNYNRSLKRLSYGLFLSHINLTGNGDRLTIYGQAGFTKKI
                     SIDYDYPYLNHSNTLGIGFTGFYAANREVAYKTIDNFLVLKRNLDQDLLKRVKLGARM
                     FYRPAIYAKHLVEINYYHFNINDSLTTSYNPDYLNNRSKLSFPEFIYLFRYDTRDVVP
                     YAMKGWSLGLYFSQIGLSRHDIHKSDAGLLLSGFFKITPKLSLEEHTLGRYNLDRGKV
                     PYYFNKAIGYDDIYVRGYDLNVIDGQDYFLLKNIARYKVLGKELDWGKYMVLKAYRKM
                     PLDVYLTGGFDIARVADRFYAYGNPLTNKYVYGYGVGLDVVFYYNKLVRLEVSRNSSR
                     NFGFYLHFDAGL"
     gene            1707..2597
                     /locus_tag="PLG24_09505"
     CDS             1707..2597
                     /locus_tag="PLG24_09505"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HMZ25168.1"
                     /translation="MKHIVVFSKGIEEDQAPIVRELIEKCQSVGIRVSFMKEVYDSVR
                     RWMDLPASMTGIGASDLTGLKDIDFLFSLGGDGTLLEASMLTNDIPIVGINMGRLGFL
                     SSIEKKYVSQTIDRLNSGDYTLEYRSLLHFQSNEGQFESYPYALNDMTIIKRDHSSMI
                     VIHATLNGEFLNSYWADGIIVSTPTGSTGYNLSCGGPILLPTAEDFIITPIAPHNLSV
                     RPIVVPDIAQLEFTIEGRAENFLCTLDSRYEVISPDCKITIRKAPHTIPLVRLPKITF
                     TKTIRDKLQWGRDLRNYKPR"
     gene            complement(2651..3157)
                     /gene="paaJ"
                     /locus_tag="PLG24_09510"
     CDS             complement(2651..3157)
                     /gene="paaJ"
                     /locus_tag="PLG24_09510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HMZ25169.1"
                     /translation="MVMTYTGNDVIALLKEVADPEIPVLSVVDLGIIEKVIVDGNRLY
                     LGILPTYNGCPAIDLIRLMIHEKCYDAGFRDIEIEYLKTPHWTTDRISPEGMNSLREY
                     GIAAPDKSTRIDQLIHGISTIRCPKCDSSHTFLISAFGSTPCKALMQCKDCHEPFEYF
                     KCNGIPND"
     gene            complement(3151..3912)
                     /gene="paaC"
                     /locus_tag="PLG24_09515"
     CDS             complement(3151..3912)
                     /gene="paaC"
                     /locus_tag="PLG24_09515"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HMZ25170.1"
                     /translation="MIENQLPIFRFLVQLGDDALILSQRLSEWCGHGPVLEQDIALTN
                     ISLDILGQARLWYQLACDHTSESMTEDKIAYKRDSAQMTNLTLVEQPNGDWGRTVMRQ
                     YLYDSFKKMYYSRLSETHYASIGEIAHKCKTETNYHYKWSKTWVQRLSMGTEEGHTRM
                     QDALNRLWRFHHELTIPSATDLEISAEGIAPNPADLKDAYLEEISSFLTECTLTIPDD
                     KTVITGGRKGIHTEHLGYILAEMQFLQRAYPDREW"
     gene            complement(3924..4208)
                     /gene="paaB"
                     /locus_tag="PLG24_09520"
     CDS             complement(3924..4208)
                     /gene="paaB"
                     /locus_tag="PLG24_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HMZ25171.1"
                     /translation="MSSNWPLWEVFIRSKNGLQHKHVGSLHASDAKTALQNARDVYTR
                     RMEGNSIWVIPSNFITASDPQEDEEFFEPAESKIYRHPTFFELPDEVKHM"
     gene            complement(4271..>4811)
                     /gene="paaA"
                     /locus_tag="PLG24_09525"
     CDS             complement(4271..>4811)
                     /gene="paaA"
                     /locus_tag="PLG24_09525"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HMZ25172.1"
                     /translation="AIMNQVPLCRTSFGPYARAMVRICKEESFHQRQGYDILLALCRG
                     SQEQKAMAQDALNRWWWPALMMFGPEDKDSVHTQQSMNWRIKRFTNDELRQRFINMTK
                     PQADFLGLTIPDPDIRWNEEKQGYDFGPINWDEFWQVVKGYGPCNAERIEARKKAWDD
                     GRWVREAAVEYAKKYENQQ"
BASE COUNT         1281 a         1040 c         1082 g         1408 t
ORIGIN      
        1 aatgtttaag gagagtgaaa actcaaaata tacttccgac aacgaaaagg caatgattta
       61 cctgcaacag gccaatcttg cagcagtcaa gggcaactac agggaagcag cgacattggt
      121 caagaaagca gacgaactca aggtgaccga atcaatgatc aaagaccagg tcaagcagtt
      181 taagcaagca ctgggcaaat cgggacaatt aaatatgcag aatcgtatgg ccatgtcaca
      241 gcgtggggga agcaagcaac gcaggccaaa gatgcgttga ttcatctcgt gcagttcagg
      301 attctccatc aaaagcaata tttgggaaga tatacgttct tcttcttttt ctttgtagcc
      361 tcttattgcc tttcagggca aaaggttgct atagatagta tttcgatcag cggactggag
      421 aaaacacggg aatacatact tattcgtgaa ttgaatttca agaagggtga ttcattggat
      481 ctgcaatacc ttgcttctat ctttcaaacc aatgaaaaac gacttctgaa taccaatctg
      541 gtggtggagg cgagatgcaa tcttatcaac ctgaatatcg aggcacaatc ggccggtgtc
      601 aacattcatt gcaaagaggc tttatatatc tatcctgcac ccttgctgga atttatcgac
      661 aatgatttca acaaatggtg gcgcaattac aatcgatctt tgaaacgttt atcatatggg
      721 cttttcctaa gtcatatcaa ccttaccggc aatggtgatc gcctcacgat atatggtcag
      781 gcaggattta ccaagaaaat ttcaattgat tacgattatc cttatctcaa tcattccaat
      841 acccttggaa taggattcac aggcttttat gccgccaaca gagaagtcgc ctataagacg
      901 attgacaatt ttctggtatt aaagcgcaat ctggatcagg acctgctcaa aagagtaaag
      961 ttaggggcac ggatgtttta taggcctgct atctatgcaa aacatttggt ggagataaac
     1021 tattatcatt tcaatataaa tgattcgttg accacgagtt acaacccgga ctatctcaac
     1081 aacagatcta aactgagctt tccggaattt atctatttat tccgctatga tacccgggat
     1141 gtagttcctt atgccatgaa aggatggagt ctgggcttat attttagtca gattggatta
     1201 agccgccatg atatacacaa gtcagatgca ggacttctct tatcagggtt ctttaaaatt
     1261 actccaaagc taagtctcga ggagcacacc ctgggccgat acaatcttga tcgggggaaa
     1321 gtaccctatt atttcaataa ggcaattggt tacgatgata tttatgtccg tggttacgat
     1381 ctgaatgtaa tcgatgggca ggattacttc ctgttgaaaa acattgcgag atacaaggtc
     1441 ttgggcaagg agttggattg gggaaaatat atggtgctca aagcgtacag gaaaatgcct
     1501 ttggatgtat atttgaccgg aggatttgat attgcaaggg tcgccgacag attttatgct
     1561 tacgggaatc cgctgaccaa taaatatgtt tatggttacg gtgtaggcct tgatgtggta
     1621 ttctattata acaaattggt aagattggaa gtttcacgca acagcagtag gaatttcgga
     1681 ttttacttgc attttgacgc tggattatga agcatattgt tgttttttcc aaaggaatcg
     1741 aagaagatca ggctccgatt gtcagggaat tgattgaaaa atgccaatct gtcgggatca
     1801 gggtttcctt catgaaggaa gtttatgact cagtccgacg atggatggac ctgcctgcct
     1861 caatgaccgg aataggagcc tcggatttga ccggattgaa ggacatcgat tttttgtttt
     1921 ctttgggtgg ggatggcact ttgttggaag cgtccatgct taccaatgat atcccgatag
     1981 tcggtatcaa tatgggtaga ttgggttttc tgtccagtat tgagaagaag tatgtcagcc
     2041 agactatcga tcgcctcaat tccggtgatt atacgcttga atacaggtct cttctgcact
     2101 ttcagtccaa tgaaggccaa tttgaatcct acccgtatgc actcaacgat atgaccatca
     2161 tcaagcgtga tcactcatcg atgatcgtga tacacgccac attaaatggg gagtttctca
     2221 attcttattg ggccgacggt atcattgttt ccaccccgac aggtagtacc ggctataatc
     2281 tcagttgtgg cgggcccatc ctgcttccaa ctgcggagga cttcatcatc acgcctatcg
     2341 ctccgcacaa cttaagtgta aggcctattg tggtacccga tattgctcag ctggaattta
     2401 ccatcgaagg cagagcagag aactttcttt gtacactgga tagcaggtat gaagtcatct
     2461 ctcccgattg taagattacg atccgaaagg caccccatac catccctctt gtgaggcttc
     2521 caaagattac atttaccaag acgataaggg ataaattaca gtggggacgt gatttgcgaa
     2581 attataagcc gagataagga ctttacggaa ggcttgaatt tcgtattgaa attggcattt
     2641 gcaaatccgg ctaatcattt ggtatcccat tgcatttgaa atactcaaat ggttcatgac
     2701 aatccttgca ttgcatcagt gctttacatg gcgtggatcc gaatgccgaa atcaggaacg
     2761 tatgggagga atcgcatttt gggcacctta ttgtagaaat tccatggatg agctggtcaa
     2821 tcctggtgga tttgtccggg gctgcgatac cgtactcccg gagcgaattc ataccttccg
     2881 gacttatccg gtctgtcgtc cagtgtggtg tcttcaggta ttctatttcg atatctctaa
     2941 accccgcatc atagcatttt tcgtggatca tcaaccttat cagatcgatg gccgggcaac
     3001 cattgtaggt aggtagaatg cccaaataga gacgattacc gtctacaatt accttttcta
     3061 taattccaag atctactaca gacaacacgg gaatctccgg atctgctact tctttgagca
     3121 gagcgatcac atcgttcccg gtatatgtca ttaccattcc cggtcaggat aagctctttg
     3181 taaaaattgc atttcagcca gaatgtagcc cagatgttcg gtatgaatac ccttcctgcc
     3241 tcctgtaata accgttttat catcaggtat ggtcagggtg cactcagtca gaaaactcga
     3301 tatttcttct aaatatgcat ctttcagatc ggctggattg ggagctatgc cttcagcgga
     3361 aatctccaga tctgtggcac tcgggatagt aagctcatga tgaaagcgcc atagccgatt
     3421 gagtgcgtcc tgcatcctcg tgtgaccttc ttcggtgccc atgctcagtc gctgtaccca
     3481 agtcttgctc catttgtaat gataattggt ctctgtcttg catttgtgag ctatttcacc
     3541 gatcgacgca taatgagtct cactaagccg ggaataatac attttcttga aactatcgta
     3601 aagatattgt cgcatcactg ttcgtcccca gtcgccgttt ggttgctcta cgagagtcag
     3661 attggtcatc tgggctgaat ctcttttgta ggcgatttta tcttcggtca tgctttctga
     3721 agtatggtca caggccagct ggtaccaaag ccgtgcctgt cccagtatat cgagtgaaat
     3781 attggtcaat gcaatgtctt gctccaggac gggtccgtgg ccacaccatt cggaaagacg
     3841 ctggctaagg atgagggcat catcacccaa ttgtaccaga aatcggaata ttggaagttg
     3901 attttcgatc atttctaatg aatttacatg tgtttgactt catccggcag ttcaaagaag
     3961 gtcggatgtc tgtatatctt actttccgcc ggttcgaaaa attcttcatc ttcctgaggg
     4021 tcggatgccg tgataaagtt ggaagggata acccagatgc tgttgccttc cattcttctg
     4081 gtatatacat cgcgggcatt ttgtaatgcc gtttttgcat ctgaagcatg caggctgccg
     4141 acatgtttgt gttggagtcc gttcttgctt ctgataaaaa cttcccaaag tggccaattg
     4201 ctgctcatcg catatttttt ttaaggttag tttacaaatg attgattatg aagatattct
     4261 aacaggaaaa ttattgttga ttttcgtatt ttttggcata ttcgacggct gcttcacgta
     4321 cccaacgtcc atcatcccag gcttttttcc tcgcttcgat acgttcggca ttacacggac
     4381 cgtaaccttt gactacttgc cagaattcat cccagttgat cgggccaaag tcgtaacctt
     4441 gcttttcttc attccacctg atatctggat cgggaatggt cagccccaga aagtcagcct
     4501 gtggctttgt catattgata aaccgttgcc gcagctcatc gttggtaaac cgcttgatcc
     4561 gccaattcat gctttgctgg gtgtgcacag aatccttatc ttccgggcca aacatcatca
     4621 gtgccggcca ccaccatctg ttgagtgcat cctgggccat agctttttgc tcctgagatc
     4681 cacggcacaa agcaagtagg atgtcataac cttgtcgctg gtggaaggac tcctccttac
     4741 aaatcctaac catagctctg gcatagggtc cgaaggaagt acgacacaaa ggcacctggt
     4801 tcatgattgc g
//
LOCUS       DAOQQN010000253         4759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8288_length_4759_cov_5.274447, whole genome shotgun sequence.
ACCESSION   DAOQQN010000253 DAOQQN010000000
VERSION     DAOQQN010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8288_length_4759_cov_5.274447"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1484)
                     /locus_tag="PLG24_09530"
     CDS             complement(18..1484)
                     /locus_tag="PLG24_09530"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HMZ25173.1"
                     /translation="MADQKKKLYSVSGRPIAENQNVKTAGKRGPMLLEDYWFLEKLAH
                     FDREVIPERRMHAKGSGAFGTFTVTNDITQYTKAKIFSEVGKKTDVFVRFSTVAGERG
                     AADAERDIRGFAMKFYTEEGNWDLTGNNTPVFFLRDPYRFPDLNKAVKRDPKTNLRSA
                     NHNWDFWTLLPEALHQITITMSERGIPKSYRNMNGYGSHTFSLINADNERIWVKFHFK
                     TAQGIQNLSNEEAAELIGKDRESHQRDLFDAIERGDFPKWNMKIQVMTQDQAKKCPFN
                     PFDLTKVWPHGDYPLIDVGVLELNRNAENYFADVEQAAFNPANVVPGIGFSPDRMLQA
                     RLFSYGDAQRYRLGVNHYQIPVNKPQCPFHNASRDGAMRVDGNEGGTLHYFPNSYNQW
                     EEQKEFTEPALSLEGDADHYDFREDDDDYYSQPGNLFRKMNDTQKQLLFKNTAAEVGG
                     AERFIQERHIANCYKADPDYGKGVADALGIAIEDALNK"
     gene            2329..2835
                     /locus_tag="PLG24_09535"
     CDS             2329..2835
                     /locus_tag="PLG24_09535"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HMZ25174.1"
                     /translation="MVGVQTFACYHSSAATKNKDLSPDSLYMYVFVSETCPICQHMTL
                     QLRDLLKKYPAARCTLVFPNYTVSDSNSVEKFLNKYKLNCNFTIDYDQKLTREWGVTI
                     TPEVLIFSGDQECIYRGKLNNQFARLGERRTVITEHYAEDAIQSYLTGQPIKINKTEP
                     TGCFIIKS"
     gene            2838..4406
                     /locus_tag="PLG24_09540"
     CDS             2838..4406
                     /locus_tag="PLG24_09540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ25175.1"
                     /translation="MRKILVLASIVFCMQYAQSQTVTFAEHVAPIIYKHCTTCHRQGE
                     IAPFPLTSYEEVKNRGAFIKYVTQIQYMPPWKPDKNIRKLQNENFLSDEEIATLAAWV
                     DQGMQRGDVSKEPPVPVFPSGSQVGNPDMVVSFAQSYLHKGTGIDEYRYFVLPTNLTE
                     DKYLVALEVRPGNKKVVHHTLVWADSTDISRNADAATPEYGFEGSSNTSVAQINNQLP
                     GYVPGQKPIIYQDGLAFKIPKGSDLLLQMHYAPSATDEKDSTSINLFFSKEPPKRLVQ
                     SFVMLPVPGILQGDVFIIPPNQVKTFHGMITLPYDVTLLGVAPHMHLLGQNWHIYCEL
                     PDGKIQDALKISDWDFNWQGSYHFTKPMILPRGTKLHAFATYDNTVNNPHNPNNPPKV
                     ISWGEKTSDEMYYLPLIFLNYQPGDENLEFHDVLSNDQIKYDFVKDKIYPVYPAPAKD
                     VVTIPFTISRPQRITVEITDLNGKVLITKYNETFAGNGMFKEEINISNLVPGTYIAVV
                     KAGRNRMTQKFIKQ"
BASE COUNT         1342 a         1051 c          970 g         1396 t
ORIGIN      
        1 tactcagaag tcttaatcta tttgttcagg gcatcttcaa tagctatgcc caacgcatct
       61 gcaactcctt tgccgtaatc cggatctgct ttgtagcaat tggcaatatg cctttcctga
      121 atgaagcgtt cagcaccgcc tacttcggca gcagtattct taaacaacag ctgcttctgt
      181 gtgtcattca tttttctgaa taaattgccc ggctggctat agtaatcatc atcgtcttcc
      241 ctgaagtcat aatgatcggc atcgccctcc aaactcaagg ccggctctgt aaattccttt
      301 tgctcctccc actggttgta gctgttgggg aaataatgca aggtgccgcc ttcgtttccg
      361 tccacgcgca ttgcgccatc tctggaggca ttatgaaacg gacattgtgg cttgtttacc
      421 ggtatttgat aatggttgac ccctaagcga taacgctggg catcgccata cgaaaacagc
      481 cttgcctgca acattctgtc cggagaaaaa ccaatgcccg gaaccacgtt tgcagggttg
      541 aatgcagcct gctccacatc ggcaaagtag ttttctgcgt ttctgttcaa ctccaaaaca
      601 cccacatcaa tcagcggata atctccatgt ggccatactt tggtcaggtc aaacggattg
      661 aaggggcatt ttttagcctg gtcctgcgtc attacctgaa ttttcatatt ccacttaggg
      721 aagtcacctc tttctatggc atcgaacaag tcgcgctgat ggctttcccg gtctttgccg
      781 attaattcag cagcttcttc attggatagg ttctgaatac cttgggctgt tttaaaatga
      841 aatttgaccc aaatcctttc attatcagca tttatcagac taaacgtatg gctaccgtat
      901 ccattcatgt ttcgatagga tttgggaata cctctttcac tcatcgtaat ggtaatctgg
      961 tgcagggctt cgggtagcaa agtccagaaa tcccaattgt ggttggcact tctcagattg
     1021 gttttcgggt ctctttttac tgccttattc aaatcgggaa atcggtaagg atctctcaaa
     1081 aagaacacgg gtgtattgtt tccggtcaaa tcccagtttc cttcttcggt gtaaaacttc
     1141 atagcaaatc cacgaatatc ccgctctgca tcagcggccc ctctttctcc tgctactgtt
     1201 gaaaaccgaa caaagacatc cgtcttcttt cctacttccg aaaagatttt ggctttggta
     1261 tattgggtaa tatcattggt cacggtaaaa gttccaaatg cgccggagcc tttggcgtgc
     1321 attcttcttt cggggattac ttcccgatcg aaatgggcca gtttctccag gaaccaatag
     1381 tcttccagga gcatcggccc tcttttgcct gctgttttta cattttggtt ttctgcaatc
     1441 ggtctgcccg aaacagaata cagttttttc ttttgatctg ccataatact tgcttttaaa
     1501 ttattttatt tatatcaact aatattttac catcgaaagg gttagtcacg ggtcttacaa
     1561 tcttttgaac cgtttttgcc ccaattatca gcggggcatc tgattttcca acaggcattc
     1621 atttgcgctc tcccgtctgg ctgccattct gattgccgtt atatttttat tttttaccaa
     1681 atcattattc ctttagatta tcctgtcgtc aatacaatac cttatggatt gcttcacgca
     1741 aaagaattcg gggccaccct ttacctgatc tgccgcatta tctgtagtca aacggattag
     1801 atctgtaaag ggttgacgtt gtcaaagtat ttgcggaaaa cgcaagatgc tatccacatt
     1861 atagaatgct taaaaggggt aaattgtttg agccggcagt cattttttcc ggtatttcca
     1921 acatgggatg tttcctgatt gcctcccgga gatttaatgg atggcgcgtc ccgggacaag
     1981 gccaccaggg atgaattttt agcaaaacga tagtatcaaa ttaaacactc acccttataa
     2041 agcatcaaat agtaaccggt tgcttaacag atggcacaat tggatggttt ggccccgggc
     2101 ccataatcct attataactt gttactacga caacggatga gacggtcagt tgacaaaagg
     2161 gctaaaccga ttctagctta acaactaatc gttgagagca atgcttcctt tcacccaaac
     2221 aattgaaggg gactaggtga tatgcctaca ttatttagca cataaagaca taattataaa
     2281 gtacatttac acatgaaaaa agcggttcta ttcattttat tttcatgtat ggtcggagtt
     2341 cagacctttg cctgttacca ttcatccgct gccacaaaaa acaaggacct gtccccggac
     2401 tccctgtata tgtatgtctt tgtttcagag acttgtccta tatgccagca tatgaccctt
     2461 caactcaggg atttactcaa aaaatatccg gcagcaaggt gtaccctggt cttccccaac
     2521 tatacggtca gcgattcaaa tagtgtggaa aaatttttaa ataaatataa attgaattgt
     2581 aattttacca tagactatga tcagaaacta acccgtgaat ggggggtaac cattacgcct
     2641 gaggtgctca tattttcagg agatcaggag tgtatctatc gcggcaagct aaacaatcag
     2701 tttgccaggt taggagaaag gcgtactgta ataacagagc attatgcgga agatgccatt
     2761 caatcttact taaccggtca accaatcaaa ataaacaaaa cagaacctac gggctgcttt
     2821 atcataaaat cttaatcatg agaaaaattt tagtcttagc aagtattgtg ttttgcatgc
     2881 aatatgcaca atctcagaca gttacatttg cagaacatgt tgcacccatc atatacaagc
     2941 attgtaccac atgccacagg caaggggaaa tagcgccatt tccgctcacc agttatgagg
     3001 aggtaaaaaa cagaggggcc tttattaagt atgtcactca gatccaatat atgccgccct
     3061 ggaagcctga taaaaatatc cgaaagctgc aaaatgaaaa ctttctcagc gatgaagaaa
     3121 tcgcaaccct ggcagcatgg gtcgaccagg gaatgcagcg aggtgatgtg tccaaagagc
     3181 caccggtacc tgtatttcct tccggttcgc aggtaggcaa cccggatatg gtggtatctt
     3241 ttgcccaatc ctatctgcat aaagggacgg gaatcgatga gtacagatat tttgtattgc
     3301 cgaccaacct cactgaagat aaatatctgg tcgccctgga agtaagaccc ggaaataaaa
     3361 aagtagttca ccacacatta gtctgggctg actctaccga tatctccaga aatgcggatg
     3421 ctgccacccc ggaatatggt tttgagggat cgagtaatac gagtgtcgcg cagatcaaca
     3481 atcagttgcc cggttatgta cccggccaaa aaccaataat atatcaggat ggtctggctt
     3541 ttaaaattcc caaaggatca gacttgttgc tgcagatgca ttatgcgcct tcagcgacag
     3601 acgaaaaaga cagcacttcc ataaatttat ttttcagtaa agaaccaccg aaacggcttg
     3661 tacaatcatt tgtaatgcta cctgttccgg ggatattgca aggggatgta ttcataattc
     3721 ccccgaatca ggtaaagact tttcatggca tgattacgct gccctatgat gtgacgttgt
     3781 tgggtgtggc accccatatg catctattag gacagaactg gcatatctat tgtgagttgc
     3841 cggatgggaa gatacaggac gcattaaaga taagtgattg ggactttaac tggcaaggat
     3901 cctaccattt taccaaaccg atgattcttc caagaggcac gaaactacat gccttcgcaa
     3961 cctatgacaa taccgttaac aatccacata atcccaataa tcctccgaaa gtaatctcgt
     4021 ggggagaaaa aacatcagat gaaatgtact atctccccct catctttctg aattatcagc
     4081 cgggggatga aaacttagag ttccatgatg tattatcgaa tgatcaaatc aagtatgatt
     4141 ttgtgaagga taaaatatat ccggtgtatc cggctccggc taaggatgta gttacaattc
     4201 cctttacgat ctccaggcct cagcgaatta ccgtagaaat taccgatctg aatggaaagg
     4261 ttttaattac aaaatacaat gaaacatttg ctggaaacgg gatgtttaaa gaagaaataa
     4321 acataagtaa tttagtcccc ggcacctata tagctgttgt aaaagcgggt agaaacagga
     4381 tgactcaaaa attcataaag caataatcgg atcgttatat tttcaactct ttttctaccc
     4441 ttggataatc cggactatat tgacccaagc attatccgcg gattacacca ttcatctgta
     4501 acttaaccat gagtggaata atttggtctg tattttcaat ttgtggagct tgttcaatca
     4561 tcttaggttc aaatatttat agaatccaat gatagataaa aagcgcgaga ttttagatag
     4621 atcccgcggt ttttgttggc tcggggttag agaagttgtc gattaacggt ttcgtgcttt
     4681 gagttggtgg gcttttgaaa ccgcaaactg tctgccagca ccaacgttta ttaattgctc
     4741 aaatgtttct attcgcact
//
LOCUS       DAOQQN010000254         4758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8293_length_4758_cov_5.512014, whole genome shotgun sequence.
ACCESSION   DAOQQN010000254 DAOQQN010000000
VERSION     DAOQQN010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8293_length_4758_cov_5.512014"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLG24_09545"
     CDS             complement(<1..376)
                     /locus_tag="PLG24_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HMZ25176.1"
                     /translation="MNIKIILVVSVITVLTVFASYKSPDKDLPANEAELGEMLFFDPI
                     LSSDKTVSCGSCHKPEHGFADNIPFSFGVDSTLTTRNTPTVKNVLARTKYFWDGRAVT
                     LEEQALKPIENPDEMNLPIAEAI"
     gene            complement(387..1874)
                     /gene="rpoN"
                     /locus_tag="PLG24_09550"
     CDS             complement(387..1874)
                     /gene="rpoN"
                     /locus_tag="PLG24_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HMZ25177.1"
                     /translation="MIRQSLSQKLTQKMSPRQIQLMKLLQIPTFELEQRIKEEIESNP
                     ALEEGNNDTYEESGYSDDDMHPGDDIRMDMDDYFPDYSEDGSDYTSPADGYDPDNKQE
                     RQIASHESNLQDYLLGQLELVDFEDETDKIIGQQIIGSIDEDGYLRREPIAITDDLLF
                     SSNMSISEEQVVKVLTVIQGFDPKGVGARTLQEALLIQIRSKLNNTSELDEYKLKDLQ
                     AAEIILRDYFNEFSKKHFSKLVQNLEIDEDDLKAAYEEILKLNPKPGSGFSMGGTTSI
                     QYIEPDFIIVNRDGTLELSLNSRNSPDLKVSNGYKSMIRELQRKKKMGKLSKEDRNTA
                     LFVKQKIEAAQTFIESIQRRQSTLYNTMHAIMMYQYDYFLTGDTQYLKPLILKDIAEE
                     IGMDISTVSRVANSKYVQTEFGTKLLKEFFSEKVQNNDGEDVTTREVKQILSSIIEKE
                     DKSNPLSDEALMELLDKTGISLSRRTISKYREKMNIPVARLRKEL"
     gene            2517..4298
                     /locus_tag="PLG24_09555"
     CDS             2517..4298
                     /locus_tag="PLG24_09555"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HMZ25178.1"
                     /translation="MHTNDKLKALREEMLVHGVDAMIIPTGDPHQNEYLPEFYKSRAW
                     VSGFTGSAGTAVVTKDHAGVWTDSRYFLQAEIQFADSEFELHKLVIQGAPEYADWLAD
                     TLGAKSVVGFDANVMPLGLVDTLIEKFEAKDIQLKTDFEPFGKIWKDRPGLSREPIFE
                     HDVKYAGRSRQEKIASIREEMKKHGADYYLVTALDDIAWLLNLRGRDIECNPVFISYV
                     LFSPEKVYLFIDGNKVNAELQQSLEDDNIAIKSYDAIRHYVAEIDKQSSILISPASLS
                     YGLYKKIGTDTIIRKDSPIMHAKSLKNEVEQRHVRHAMIKDGRALAHFFIWLEEALGQ
                     GETPTEYDLTRKLIEFRSQQEGYYGESFDAIIGYKGNGAIIHYKPEEETAAAVRPNGV
                     LLVDSGGQYNDGTTDITRTIALGPVSDEIKDHYTRVLKGHIAVADVIFPKGTRGIQLD
                     TLARQFLWQTGITYNHGTGHGVGFFLNVHEPPQGIANGLGERGVTVILPGTLTSNEPG
                     YYKKDSHGIRTENLVICHEVEGNPDFLYFETVTLFPIDKTMIDISIMSPSETKWLNDY
                     HQKVYRELAPGLDDKARKWLEAKCTAL"
     gene            complement(4293..>4758)
                     /locus_tag="PLG24_09560"
     CDS             complement(4293..>4758)
                     /locus_tag="PLG24_09560"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HMZ25179.1"
                     /translation="KRMKAILLTILLFLLLVVTIIAFVLANQILQIIGLYINIDYITR
                     GLILAARWVPVFFLMYGLLTLIYRLGPSIKRKIPFFTIGTYMTTFLCILVSLLISYFV
                     NNFSNYNKIYGSLGTIAALMLWLQTNMILIFVGYELNLIINEFPRRKRSKAS"
BASE COUNT         1398 a         1037 c          962 g         1361 t
ORIGIN      
        1 tgatagcttc tgcaattggc agattcattt catcgggatt ctctatcggt ttgagagcct
       61 gctcttccag tgttacagcg cggccatccc agaaatattt tgttcttgcc aaaacatttt
      121 tcaccgtcgg cgtattgcgt gtcgtcaaag tactatctac cccaaaactg aagggaatat
      181 tatccgcaaa accatgctca ggcttatgac aggagccgca acttacagtt ttgtccgatg
      241 ataggatcgg gtcaaaaaat aacatctcac ctagctcagc ttcattggcg ggtaaatctt
      301 tatcgggtga cttataggaa gcaaagacag taagtacagt tatgacagat actacaagga
      361 taattttaat attcataaga aaatctttat aattctttac gcaacctggc taccggaata
      421 ttcatcttct cacgatactt ggatatggtg cggcgagaca gggaaatgcc ggttttgtcc
      481 agcaactcca tcaaggcttc atcactcaaa gggttggact tgtcctcctt ttcaatgatg
      541 ctggaaagga tttgcttgac ttctctggtt gttacatcct caccatcatt attctgcacc
      601 ttctcgctaa aaaattcctt cagcaacttt gttccgaatt ccgtctggac atacttacta
      661 ttggcgactc gcgatacggt ggagatatcc ataccgatct cttcagcaat atcctttaat
      721 atcaagggct ttaaatattg cgtatctccg gtcaggaagt aatcatactg gtacatcatg
      781 atggcatgca tggtgttata taatgtgctt tgccgccttt gaatcgactc gatgaatgtc
      841 tgagccgcct caatcttttg cttgacaaac aaagccgtat tcctgtcttc tttggacagt
      901 ttgcccatct ttttctttcg ttgcaactcc cgaatcattg acttataccc attactcacc
      961 ttcaaatccg gagaattacg gctattcagg gacaattcca atgtaccatc ccgattgaca
     1021 atgataaaat ccggttcaat atattggatg ctggtagtac ctcccataga gaatccactt
     1081 cccggtttgg ggttgagctt taaaatttcc tcataggcag ccttcaggtc atcttcatct
     1141 atttccagat tttgcactag tttggaaaag tgctttttgg aaaactcatt gaaatagtcc
     1201 cggagtataa tttcggcagc ctgaagatcc tttaacttgt attcatccaa ttcagaagta
     1261 ttattcaact ttgacctaat ctggatcaac aatgcctcct gcaatgtcct tgcccccact
     1321 ccttttggat caaaaccttg aatcactgta aggaccttga ccacttgctc ttccgaaatg
     1381 gacatattgg aactaaacaa caaatcatcg gtaatggcga ttggctcgcg tctcaaatat
     1441 ccatcctcat cgatacttcc aatgatttgc tggccaataa tcttgtctgt ttcgtcctca
     1501 aaatccacta gttccagttg acccagcaaa taatcctgaa ggttgctttc gtgcgaagcg
     1561 atctgtcgct cctgcttatt atcagggtca taaccatcgg caggagaggt atagtctgat
     1621 ccatcttcac tataatccgg aaaatagtcg tccatatcca tgcggatatc atcccccgga
     1681 tgcatatcat catcactgta tccggattct tcgtacgtgt cgttgttccc ttcttccaat
     1741 gcaggattcg attctatttc ctccttgatc ctttgctcaa gctcaaaagt aggtatctga
     1801 agcaacttca ttaattgaat ctgcctcggc gacatttttt gagtgagctt ctgacttaac
     1861 gattgtcgaa tcattgtcta tgtttgttct ctatattttg aaagttatgc cagcacaaag
     1921 atattataaa ttttaataat acataatcgt tcaaaaatta atatcaattt cacctttctt
     1981 acccctgctg atctgctcct tttctaccca ccctcctatc aattcaaatg aattctgact
     2041 tcacataaaa ggcatcccaa aatactgctt cctgatcttc agaacaaaaa aaacctactt
     2101 tatgtttgaa accctccaca cgtttaaagc aattatgtcc atcagattga tggaagatca
     2161 accgtgaaac tgacaagcag cgatttcaat aaccttggag ttaaaatatc aggagttcca
     2221 attttaagga tattcttaat taaaagcatt ggcaagaatt ttgcacagcc ttactttatt
     2281 ggtacttaca ttatgttgaa ataataaata atatatataa tattgaatac catgtaaata
     2341 cctttatact gttgcatatt ccgtgatcaa ttaccttcga atttcctttt attaaaagaa
     2401 gaatagtgga atatggataa acaacctaaa ggcacattta aacaattgta ttccctattt
     2461 tctttatatt tgggcaactc attgaatcaa tttacatcaa aaatattttt tacataatgc
     2521 ataccaacga taagctgaaa gctctcagag aagaaatgct ggtacacggt gtggatgcca
     2581 tgatcatccc taccggagac cctcatcaga atgaatattt acctgaattc tacaagtcaa
     2641 gggcctgggt atcgggattt accggatctg cggggactgc tgtcgtaacc aaggaccatg
     2701 ccggggtctg gactgactca aggtattttc tgcaagctga aatacaattt gccgacagcg
     2761 aatttgaatt acacaaactg gtgatacaag gtgccccgga atatgcagac tggcttgccg
     2821 acacactagg cgccaaaagt gttgtaggct ttgacgccaa tgtaatgccg ctgggattag
     2881 tggatacgtt gatcgagaaa ttcgaggcta aggatattca actaaaaacc gactttgaac
     2941 catttggcaa aatatggaaa gaccggcccg gtttgtccag agagcctatc ttcgagcatg
     3001 acgtgaaata tgcaggccgg tcaaggcagg aaaaaatcgc ttctatccgc gaagaaatga
     3061 aaaaacacgg agcagattac tatctggtca ctgctttgga cgatattgcc tggctgttga
     3121 accttcgggg tcgtgacatc gagtgcaatc cggtattcat ttcctatgtt ctattctctc
     3181 ctgaaaaagt ctatctcttc attgatggca acaaggtcaa tgctgaatta caacaaagcc
     3241 ttgaagatga caatatcgcc attaaatcct atgatgccat cagacattat gtagctgaaa
     3301 tagataagca aagctccata ttgattagtc cggcctcact ttcatacggt ctctataaaa
     3361 aaatcggcac cgatactatc atccggaagg atagccccat catgcacgcc aagtctctca
     3421 agaatgaagt ggaacagcga catgtcaggc atgccatgat caaggatgga cgtgcattgg
     3481 cacatttttt tatttggctt gaagaagcat tggggcaggg tgaaactcca actgagtatg
     3541 atctgacccg caagctgatc gaattccgta gtcagcagga aggttactat ggtgagagtt
     3601 ttgacgcaat tatcggctac aagggtaatg gcgcaatcat ccactataag ccggaggagg
     3661 aaacagcagc ggctgtccgg ccgaatggtg ttttattggt agactcaggt ggccaataca
     3721 atgatggcac caccgatatt accaggacta tcgcgctcgg tccggttagt gacgaaataa
     3781 aggatcatta tacaagggta ttgaaaggac atatcgctgt ggctgatgtc attttcccca
     3841 aaggcacccg gggcatccaa ctggatacgt tggccagaca attcctctgg caaacaggaa
     3901 ttacatataa tcatggtaca ggtcatggtg taggtttctt tctcaatgta catgagccgc
     3961 cccaaggtat cgccaatgga ttgggagaac gtggagtgac cgtcattctc ccgggcactt
     4021 tgacatccaa cgaacccggg tattacaaaa aggacagcca cggcatacgt accgaaaatc
     4081 ttgtgatatg ccatgaagta gaaggaaacc ctgattttct gtatttcgag actgttacct
     4141 tgttcccaat agacaagacg atgattgaca tcagtatcat gagtccgtca gaaaccaagt
     4201 ggctcaatga ttaccatcag aaagtatatc gggaactggc accgggacta gatgataagg
     4261 caagaaaatg gctggaagcc aagtgtaccg cactatgacg ctttagaacg ttttcttctc
     4321 ggaaattcgt taatgataag gttcaattcg tagccaacaa agatcaggat catattggtt
     4381 tgtagccaga gcatcaatgc tgctatcgta cccagggatc cgtatatctt attgtaattg
     4441 gaaaaattgt tgacaaagta actaatcagc aaggatacca gaatacacag aaaggtcgtc
     4501 atatatgtac caatggtaaa gaaagggatc tttctcttaa tggatggacc caggcgatag
     4561 ataagggtta aaagtccata catcaggaaa aagaccggca cccatcgggc cgcaaggatg
     4621 agcccgcgtg tgatatagtc aatgttgata tacagaccga tgatttgaag gatttgattg
     4681 gcaaggacga aggcgatgat ggtaaccacc aataataaaa acaacagaat agtaagtaga
     4741 atcgccttca ttcgtttt
//
LOCUS       DAOQQN010000255         4747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8326_length_4747_cov_4.585251, whole genome shotgun sequence.
ACCESSION   DAOQQN010000255 DAOQQN010000000
VERSION     DAOQQN010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8326_length_4747_cov_4.585251"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLG24_09565"
     CDS             <1..405
                     /locus_tag="PLG24_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25180.1"
                     /translation="AILEFIGEWNDALHNDIMELKRTIIDHQINHGITKFILIGEQVL
                     NFHSSDDSYYEEWYEDIKDEAGWIVLLGLLPHVMEEMAHSRLYHFMLYGEQWNQVNWR
                     AFTPSQLFILVDKFIENPKTLPESTFSHKRIK"
     gene            402..1295
                     /gene="era"
                     /locus_tag="PLG24_09570"
     CDS             402..1295
                     /gene="era"
                     /locus_tag="PLG24_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HMZ25181.1"
                     /translation="MKVHYSGYVNILGKPNAGKSTLLNQLLETRLSITNKKAQTTRRR
                     ILGIWNDEKHQIVFSDTPGIIDKPAYKMQEKMNSYIFQSFEDADIVLYLADCTDPQPW
                     SEETGRILNKAQTPKILVINKKDLRPEATIPEITSGWPNDVNWNSILLISAIDENDRK
                     IIIDNVISLLPPGPEYFPKDQLSDLNERFFVSDLIRQNILSLYSQEVPYSCEVYIDNY
                     VESEKNGKPFAHISATIFVARNTQKSILIGKHGEKIKQLGIRSRADIEEFLDHHIFLE
                     LYVKVRENWRDDEKQLKHFGY"
     gene            complement(1276..1872)
                     /locus_tag="PLG24_09575"
     CDS             complement(1276..1872)
                     /locus_tag="PLG24_09575"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HMZ25182.1"
                     /translation="MAHSFLSPDKPYVLTGNVVADLIKGSARKEVDLRFLPGIELHRD
                     IDAFTDGHPAVTRFKAALHDRFHKYAPVVSDIYMDHFLVLNWSQYSEWEIHDYIDCIY
                     NKMNSVIDQLPQDAYRRIRNMIEHRWLTVYFTIEGIEEVFSRMNHKLSDKNILKGGGT
                     WLSERIDDFNDIFISFFPELINFVSQSYPIPAFNNRNV"
     gene            complement(1943..2428)
                     /locus_tag="PLG24_09580"
     CDS             complement(1943..2428)
                     /locus_tag="PLG24_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25183.1"
                     /translation="MKYNLFSFGILSKMERLSQFFCFSSSKCLHRTGILSVLCISILP
                     ILLLAGCRDRKSFSIDNMINSKIKFDVSQLDKDGLAGSEDGKYSITYEFCIPDTRLYR
                     DKVKKIDSTIVFTKSPGRSMCDKNQILCLGNTHQPHAVKVLNKLAGLSFVEKISETYF
                     E"
     gene            complement(2470..3012)
                     /locus_tag="PLG24_09585"
     CDS             complement(2470..3012)
                     /locus_tag="PLG24_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25184.1"
                     /translation="MFVFLTVFIVGMMHFRALGQKRPTSKKSVPVARVVPQTPFNFLI
                     GNWASYSQEATFAAYCNVKAIASGTALEIHLEKPDKTMSVGLIYSDALDDRWKMVWVG
                     PDVNTMHSNGVRYYESSYDRNLYFFQGKTIFNGKSVKDRFMFENINNDTVVFSYELST
                     DNEKTWDIEFRFTFKKNNSQ"
     gene            3347..4000
                     /locus_tag="PLG24_09590"
     CDS             3347..4000
                     /locus_tag="PLG24_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HMZ25185.1"
                     /translation="MIEAIKKIFDFILHIDVHLTEIVSNYGTLTYVVLFLVVFCETGL
                     VVTPFLPGDSLFFAAGAISASSGLNVVLTIVVCMVAAIVGNTVNFAIGKRVGPAIFNK
                     ESRFIKKEYLEKTKAFYDKHGTKAIVLSRFLPIFRTFVPFIAGIAGMNPFKHLVYNIV
                     GAVLWVNIFVLGGYFFGSIPFVQNNFSLVIIGILVVTVLPAVITVIKAKFFPDKEVL"
     gene            complement(4076..>4747)
                     /locus_tag="PLG24_09595"
     CDS             complement(4076..>4747)
                     /locus_tag="PLG24_09595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HMZ25186.1"
                     /translation="FSVQSHQKAAAAIDAGNFTDEIVPIDVEEVYVDGGSRKTKSYTV
                     SVDEGVRRDTSMEGLAGLKPAFTNGGTVTAGNSSQTSDGASFVLVVSEEYVKQFNLTP
                     VARLVSCSVAGVEPIHMGIGPCAAVPKALAQGGLTLSQIGLIELNEAFAAQSLAVIRE
                     LNLDIAKVNVNGGAIALGHPLGCTGAKLSVQVINEAKRRNEKYTMVTACVGGGQGIAG
                     IYEVF"
BASE COUNT         1473 a         1000 c          905 g         1369 t
ORIGIN      
        1 gccatcctgg aatttatcgg tgaatggaat gacgccttgc acaacgacat catggaactg
       61 aagcggacaa tcatagatca tcagatcaac catgggataa caaaattcat tcttatcggc
      121 gaacaagttc tgaattttca ttcatcggat gatagttatt atgaagaatg gtacgaggat
      181 atcaaagacg aagcaggctg gattgtactt ctcggcttgc tcccccatgt gatggaagaa
      241 atggctcatt cacgtcttta tcattttatg ttatatggag aacaatggaa tcaagtcaac
      301 tggagggcat tcactccatc ccaactattt atccttgttg ataaattcat cgaaaatcca
      361 aagaccctac ccgagtctac gttttcacac aaaagaatca aatgaaggta cactattccg
      421 gatacgtcaa tatcctcggc aaacccaatg cgggtaaatc aaccctgctc aatcagctac
      481 ttgaaacacg cctaagtatc actaacaaaa aagcccagac tactcgcaga agaatcttag
      541 gcatatggaa tgatgagaaa catcagatag tcttttctga cactccaggc attatcgata
      601 aaccggcgta taagatgcag gaaaaaatga acagctacat ctttcaatca tttgaagatg
      661 cagatattgt actttatctg gctgactgca cggatcctca accctggtca gaagaaacag
      721 gcagaattct gaacaaagct caaaccccta aaattttagt catcaataaa aaagacttac
      781 gacctgaggc aactatcccg gaaatcactt cgggttggcc aaatgatgtc aactggaatt
      841 caatacttct gatatcagca attgatgaaa acgacagaaa aatcatcatc gacaatgtca
      901 tcagcctact cccccccggt ccggaatatt ttccaaagga tcaactcagc gacctcaatg
      961 aaaggttttt tgtctctgac cttatccgtc agaatatcct ttccttgtat agccaggagg
     1021 tcccatattc atgtgaagtg tatatcgata attatgttga atctgaaaag aacggcaagc
     1081 cttttgcgca catcagtgca accatctttg ttgcaaggaa tacccagaaa tcaatattga
     1141 tcggtaagca tggagaaaaa ataaaacaac tgggaattcg ttcgagagct gatatcgaag
     1201 aatttcttga tcatcacatt ttcctggaat tatatgttaa ggtcagggaa aactggcggg
     1261 atgatgaaaa acaacttaaa catttcggtt attaaaagcg ggtattggat aggattgtga
     1321 tacaaagttt ataagttcag ggaaaaatga aataaaaata tcattgaaat catcaatcct
     1381 ttcagacaac caagttcctc ctcctttgag tatatttttg tctgatagtt tgtgattcat
     1441 ccttgaaaaa acctcttcaa taccttcgat tgtaaaatac accgtgagcc atcgatgctc
     1501 tatcatattc ctgatccgtc tgtatgcatc ctggggaagt tggtcgatga ccgaattcat
     1561 tttgttgtat atgcaatcga tgtaatcatg aatctcccat tcagagtatt gagaccagtt
     1621 caatactaaa aaatgatcca tatatatgtc gctcaccacc ggggcatatt tatggaatcg
     1681 atcatgcaag gctgccttaa atcgagttac cgccggatga ccatctgtga atgcatctat
     1741 atcccggtgc aactcaatac cggggaggaa tcgcagatcc acttctttcc tggcacttcc
     1801 cttgatcagg tcagccacta cattgcccgt tagtacatac ggtttatcag gtgacaaaaa
     1861 agaatgcgcc aaataattca tttaaagatt tattgttaat tgcatgactg taaaaggcac
     1921 aacgaaattg tactcaatct ggctattcaa agtaggtctc actgattttt tcaacaaaac
     1981 tcaacccggc aagtttattc aaaactttta ctgcatgagg ttgatgggtg ttacccaaac
     2041 aaaggatttg attcttatcg cacatggatc taccaggaga tttggtaaaa acaatagttg
     2101 aatcaatttt cttaacctta tctcggtaca agcgggtgtc gggaatgcaa aattcatagg
     2161 taatgctgta tttaccatcc tcactacccg ccaggccatc cttgtccagt tgactgacat
     2221 caaatttaat ctttgaattg atcatattgt cgatgctgaa tgacttacgg tcccggcatc
     2281 ccgcaagtag tagaattgga agaatcgaaa tacacagcac ggatagtata cccgttcgat
     2341 gtagacattt ggaggaagag aaacaaaaga actggctcag tctttccatt ttactcaaaa
     2401 taccaaagga gaaaagattg tatttcatat tggcagggaa ttacactgat tgttctcgaa
     2461 aaacaaacat cattgtgaat tatttttttt gaaagtaaac ctgaattcta tatcccaggt
     2521 cttttcatta tccgtactta actcataact aaaaacgaca gtatcattat taatattttc
     2581 aaacatgaaa cggtctttca cggactttcc gttaaatatg gttttgccct ggaaaaaata
     2641 aagattgcga tcataagagg actcatagta tctgactccg ttgctatgca tggtattgac
     2701 atcgggcccc acccaaacca ttttccaacg atcatccaaa gcatcagaat aaataagccc
     2761 aacggacatt gttttgtccg gcttttccag gtggatctct aaggcagtgc ctgaagctat
     2821 tgccttgaca ttacaatacg cggcgaaagt ggcttcctga ctataggacg cccaattacc
     2881 tatgaggaaa ttgaatggcg tttgaggtac aacccgagct accggtaccg atttcttcga
     2941 agttggcctt ttttggccca aggcccgaaa atgcatcatt ccgactataa aaacagtcag
     3001 gaaaacgaac ataaagggca ttttacaaat cataggtcaa gaatatggct tatcattcga
     3061 ggtgcaaaag tagtaaaacc aacgtgaata ccataaatat tatcagtatc aatctggtga
     3121 tacaaggata atatcagatg aggcgtacta actacccaaa atgcttaaaa ttaaatatag
     3181 gttttggtgg gtaatagatt gtgatgggta ttaattaagc taaattggga aggaccggca
     3241 ccgacaattt atttacctat gaatttttat acgaaatttc acctttattt aattccgtat
     3301 aaataaatgc ccattttttt catctttgca attcaaaagc taagaaatga ttgaagcgat
     3361 taaaaaaata tttgacttca tactccatat tgatgtccat cttacggaaa tcgtaagtaa
     3421 ttatggtaca ttgacttatg tagttttgtt tttggtagta ttttgtgaaa ccgggcttgt
     3481 tgtgacaccc tttttacccg gggattcgtt gttttttgca gcaggtgcga taagtgcatc
     3541 atcaggtctt aacgtagtgc tgaccattgt tgtatgtatg gtagcagcta ttgtgggaaa
     3601 taccgtaaac tttgccattg gaaagagagt aggacctgcc attttcaata aagaatccag
     3661 atttatcaaa aaagaatatc ttgaaaagac caaagccttt tacgacaaac atggaacaaa
     3721 agccatagta ttaagtcgat tcctcccaat attcagaact tttgtcccct tcattgctgg
     3781 cattgcaggt atgaacccct ttaaacacct ggtttacaac atcgtcggag cggtcttatg
     3841 ggtaaatata ttcgtattgg gtggatattt cttcggcagt attccattcg tccagaataa
     3901 tttcagtcta gtcatcattg gcatccttgt tgtaacggtt ttgcctgccg ttataactgt
     3961 aatcaaagca aaattttttc ctgataagga agtactttag gaataaggaa ataaaaaatg
     4021 cagtgccgga ataatttggc actgcatttt cagtctaaag acaatagtat tccaatcaga
     4081 atacttcgta aatccctgct atgccctggc caccgcctac acaggctgta accatcgtat
     4141 atttttcatt tcttcgtttg gcttcattga ttacctgaac agacaacttg gcacctgtac
     4201 aacccagtgg gtgcccaagg gcaatagctc caccgttgac attgacctta gcaatgtcca
     4261 aatttaattc ccgaatgaca gcgagcgact gtgctgcgaa tgcctcattc agttcaatca
     4321 acccaatctg actcagggta agtcctcctt gagccaaagc cttcggaact gccgcacatg
     4381 gccctatgcc catatgaata ggttctacac ctgcgacact acatgaaacc aacctggcaa
     4441 ccggagtcag gttaaactgc tttacatatt cttcgctaac caccagtaca aatgacgctc
     4501 cgtctgatgt ttgggaggaa tttccggctg ttaccgttcc tccattggta aatgccggtt
     4561 ttagtccggc aaggccttcc atggaagtgt ctcgtctgac gccctcgtct accgataccg
     4621 tataggattt agttttccgg ctcccaccat ctacatagac ctcttctaca tcaatcggaa
     4681 ctatttcatc agtaaaatta ccggcatcga tggccgctgc ggctttttgg tgagattgca
     4741 cagaaaa
//
LOCUS       DAOQQN010000256         4735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8356_length_4735_cov_5.418590, whole genome shotgun sequence.
ACCESSION   DAOQQN010000256 DAOQQN010000000
VERSION     DAOQQN010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8356_length_4735_cov_5.418590"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1394
                     /gene="ftsA"
                     /locus_tag="PLG24_09600"
     CDS             3..1394
                     /gene="ftsA"
                     /locus_tag="PLG24_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HMZ25187.1"
                     /translation="MGTTKVCAMAGRRDLFGRLEILAVGKVDSDGVLRGVVSNIEKTV
                     RAISDAVRIVEQKLQKTVTTVHVGIAGQHIKSVQHRGSKLRDNADVEISRQDIRQLIM
                     DMYKLNLPPGDKILHVYPQEYSVDNEQGVVDPIGMSGVRLEANFHIITGQITAFNNIV
                     RCVEKAGLRLADLTLEPIASASSVLSEEEKEAGIALVDIGGGTTDVCVYYDGIIRHAA
                     IIPFGGNAVTRDIKEGCNVMLNQAEKLKTKFGYALADEMYDDKIITIPGLKGRENKEI
                     SQRNLALIIQARMEEILDYVMLEIEKSGYERKLTGGIVLTGGGALLNGVDKLTALHSG
                     LESRIGLPIEFLAHGYNANVSNPIYATTLGLLMEAIGHVTMDEVEDKLPAGRDTSRYE
                     VELVGTPYSKDSVLQAGAPQDTPTLGDPTQIQGEAGANPENEGIEQSGWMRRVWDKIR
                     NTMGNDWDDEDLR"
     gene            1453..2874
                     /gene="ftsZ"
                     /locus_tag="PLG24_09605"
     CDS             1453..2874
                     /gene="ftsZ"
                     /locus_tag="PLG24_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HMZ25188.1"
                     /translation="MPILEFDLPKSDQSIIKVIGVGGGGSNAVNHMFNQGIIGVDFAI
                     CNTDNQALAISPIPTKIQLGPGLTEGRGAGSRPEVGKHACLESIEDVQLFLSSHTKML
                     FITAGMGGGTGTGAAPIIAKAAREMGILTVGIVTLPFAFEGKLRHRYAQEGLEELKQN
                     VDSLIVISNDKLREIHGNLTISEAFSQADNILTTAAKGIAEIITIPGYINVDFEDVNT
                     VMRNSGVAIMGSAQAEGDDRARRAVDMALNSPLLEDNNISGAQNVLLNITSGSQEATM
                     SEISEITEFVQAEAGINTNIIWGNCYDESLGNKISVTLIASGFEKTGKNVRQSQRHTF
                     QLDDVKETAKADNRLYLDDDPFGELDATHEQLKSTANRYVQTNVSDPYMSRSEREQEE
                     QRKLHELEHQKAELRKSASRVKLSNPVTIVELENTPAYVRRRVVLEEVPHSSERNYSK
                     WVINSDDELIMRNDNPFLRDLAD"
     gene            complement(3072..3530)
                     /locus_tag="PLG24_09610"
     CDS             complement(3072..3530)
                     /locus_tag="PLG24_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ25189.1"
                     /translation="MEIVIRKAVSGDASAIMTLVHELAAYEHAAHEVTVDFDHFMESG
                     FGPDPVWQAFVACQGEIIIGLALYFVRYSTWKGQRMYLEDLVVNPAHRGKGIGSLLMD
                     SLIQEAENRQFSGINWQVLDWNESAINFYRKYKPTFDNGWINVSLDIPIP"
     gene            complement(3605..4588)
                     /locus_tag="PLG24_09615"
     CDS             complement(3605..4588)
                     /locus_tag="PLG24_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HMZ25190.1"
                     /translation="MQNILDVIIVGGGPIGLACALEAQKARLDYLILEKGCLVNSLFH
                     YPREMTFFSSSELLELDQIPFVSVHAKPRRAEALEYYRRIARNRNIHMRLFEKVEQIE
                     KSPTGCFITTTGSNSYSSKNIIIATGFYDLPYMLGVEGEELPKVKHYYDDAHYYSFQK
                     VAVIGAMNSAVDAALETWRKGAEVTMIIRGHEIGERVKYWIRPDIENRIKEGSIKAYF
                     DSQVTRITQDSIEFRTQDGIKSIPNDWVLAMTGYQPNLKFLVSCGIELSPDSQQKPSY
                     NERTYESNVPGIFLAGVVCGGMDTHSYFIENSREHARIIIETISRRQKAII"
BASE COUNT         1346 a          970 c         1099 g         1320 t
ORIGIN      
        1 acattggcac caccaaagtg tgtgcaatgg ccggtcgtcg ggaccttttt ggccgcctgg
       61 agatattggc cgtcggaaag gtagattctg atggcgttct tagaggagtc gtcagtaaca
      121 tcgagaaaac ggttagagcc atctccgatg ccgtgagaat tgttgaacaa aagctccaaa
      181 agacggttac aaccgtccat gttggcattg ccggccaaca catcaagagc gtccagcatc
      241 gtggttcaaa actccgtgat aatgctgatg tggaaatatc acggcaggat attcgccagt
      301 tgatcatgga catgtacaag ctcaatcttc cgccgggaga taagattctg cacgtatacc
      361 ctcaggaata tagtgtggac aatgaacagg gtgtagtcga tcccatcggt atgagtggag
      421 ttagactgga agccaatttt catatcataa ccggtcagat cacggctttc aacaatatcg
      481 taagatgtgt cgagaaagcc ggcctgcgct tagcagacct gacacttgag ccgatcgctt
      541 ccgcttcttc cgttttaagc gaggaagaga aagaagcagg catagctctg gtggatatag
      601 gtggaggcac gaccgatgtc tgtgtatatt atgatggaat catcaggcat gctgctatca
      661 ttccttttgg aggaaatgcc gtgaccagag atataaagga aggttgtaat gttatgctca
      721 atcaggctga aaagttaaag actaagtttg gttatgcact tgcggatgaa atgtatgatg
      781 ataagattat taccattccg ggcctgaaag gaagggaaaa taaagagatc agtcagcgca
      841 atctggcctt gatcattcag gcccgtatgg aagaaatttt ggattatgta atgcttgaaa
      901 ttgaaaagtc agggtatgaa agaaaactga ccggaggaat cgtgcttaca ggtggaggag
      961 ctctgttaaa cggagttgac aagcttactg ctttacacag tggtttggag tcgcgtattg
     1021 gcctgccaat agaattcctt gctcatggat ataatgccaa tgtctccaac ccgatttacg
     1081 caaccacctt aggtttgttg atggaggcaa ttggccatgt gacgatggac gaagtcgagg
     1141 ataaacttcc tgccggaaga gatacgagca gatatgaggt ggaattggta ggtacgccat
     1201 attcgaagga tagtgtatta caagccggag cgcctcagga tacgcctact ttgggtgatc
     1261 ctacacagat tcagggtgaa gcaggtgcca atcctgaaaa tgaggggatc gaacagtccg
     1321 gatggatgag acgtgtttgg gacaaaatcc gtaatactat gggtaacgat tgggatgatg
     1381 aagatttaag ataatctcat gaagctcaat tttttttcga aatctatttt taataattca
     1441 aaattaaaga aaatgccaat tttagaattc gatctgccta aatcagatca atccataata
     1501 aaagtcatcg gtgttggtgg cggaggaagc aatgctgtca atcacatgtt caatcagggc
     1561 atcattggtg tggactttgc aatttgcaat acagacaatc aggcgcttgc catcagtcca
     1621 attccaacaa agattcagtt gggacccggt ctgacagaag gccgtggagc cggctctcgg
     1681 ccggaagtag ggaagcatgc ttgtctggag tccattgaag atgttcagtt atttctttca
     1741 agtcatacca agatgttgtt tatcactgcc ggaatgggcg ggggaacagg aacaggagct
     1801 gcacctatca tagccaaggc tgcccgtgag atgggcatcc tcacagtcgg aatcgttaca
     1861 ttgccttttg cctttgaagg taaacttaga catcgatacg ctcaggaagg actcgaggag
     1921 ttgaagcaaa atgttgactc tctgattgtg atctccaatg acaagttacg cgagatacac
     1981 ggcaatctta caatttccga agcttttagc caggcagata atattctgac gaccgctgcc
     2041 aaaggtattg ctgagattat taccatcccg ggctatatca acgttgactt tgaagatgtc
     2101 aatacggtaa tgcgcaacag tggggtggca atcatgggtt ccgcccaggc tgaaggcgac
     2161 gatagggcaa ggcgtgctgt tgatatggcc ctcaatagtc cgctgttgga ggataataat
     2221 atttcaggtg ctcagaatgt cttgttgaac attacttcag gctctcagga ggcgacgatg
     2281 tctgaaatat ccgaaattac ggaattcgta caggcagaag ccggaatcaa taccaatatc
     2341 atctggggta attgttatga tgagtccctg ggtaataaaa tcagtgtcac attgatcgcc
     2401 agtggttttg aaaaaaccgg taaaaatgta cgtcaatctc aaagacatac tttccaattg
     2461 gatgatgtta aggaaactgc aaaggctgat aacaggcttt atctcgacga cgatcccttt
     2521 ggtgaattgg atgccactca tgaacaatta aaatccactg ccaaccgata cgtacagact
     2581 aatgtatctg atccgtacat gagccgttct gaaagagagc aggaagaaca gcgtaagttg
     2641 catgagttgg aacatcagaa agcagaactc cggaaaagtg cttcaagagt aaagctaagt
     2701 aatcctgtaa ccatcgttga gctggaaaat actccggcct atgtaagacg aagagtggta
     2761 ctggaagaag ttccacactc atctgaacgc aattacagca aatgggtaat taactctgat
     2821 gatgaattaa tcatgcgcaa tgacaatccc tttctgaggg accttgcaga ctgatagaca
     2881 agtgttggct ttgacacccg aaatgcctga caatgccagg cactttaccg gagtatgtcc
     2941 atgactccgg cttgaataag attgttttta ttagttaggt ttattgttta taagggcggc
     3001 tctctcaaag tcgctctttt ttttaattgt cagtatgaaa tacttttctt tgttatggag
     3061 gtggtgtaac cttaaggaat tgggatatcc agactgacat tgatccaacc gttatcaaaa
     3121 gtgggtttgt attttctgta aaaattgatt gccgattcgt tccagtccaa aacctgccag
     3181 ttgattccgg aaaactgtct gttctcagct tcctggatta ggctgtccat caataaggac
     3241 ccgattcctt tacccctgtg tgccggattc actaccagat cttccaaata cattctttgt
     3301 cccttccagg tagaatatcg aacgaaatac aaggccaggc cgatgataat ctccccttgg
     3361 catgcaacaa atgcctgcca tacaggatcg ggcccgaaac cactttccat aaagtgatcg
     3421 aaatcaacgg tcacttcatg tgctgcatgt tcgtaggctg ccaactcatg taccaatgtc
     3481 atgatggctg acgcatcccc acttacagct tttcttatga cgatttccat gacgtgaaga
     3541 taggaattaa tagaaagaaa ggaaaaatcc cattccaaca ttttcccttc tccgggttaa
     3601 agtatcaaat tattgctttt tgccttcggg aaatcgtttc gatgatgata cgtgcatgct
     3661 ctctgctgtt ctcaatgaag tagctgtgtg tatccattcc tccacaaacc actccggcaa
     3721 ggaaaattcc gggtacatta ctttcataag tacgttcatt ataggatggc ttttgttggc
     3781 tgtcaggact cagctcgatt ccacaagaaa caagaaattt tagattgggt tggtaccccg
     3841 tcattgccag cacccagtca tttggtatac ttttgatgcc atcctgtgtc ctgaattcaa
     3901 tggagtcctg agttatacgg gtgacctgac tgtcgaaata tgccttgata gatccttctt
     3961 ttatcctgtt ttcaatatcc gggcgaatcc aatatttgac tctttctcca atttcatggc
     4021 ctcgaatgat catggtgact tcggcacctt tacgccatgt ctcaagtgca gcatcaaccg
     4081 cggaattcat ggctccaatt actgctactt tctgaaacga atagtaatgg gcatcatcat
     4141 agtaatgctt tacctttggt agttcttcac cttctacgcc aagcatatag ggtaaatcat
     4201 aaaatccggt ggcaatgatg atattttttg acgaataact gttactgccg gttgtggtga
     4261 taaaacatcc ggtagggctt ttttctattt gttcgacttt ctcgaataac ctcatatgga
     4321 tgttccgatt cctggctatc ctcctgtagt attccaatgc ttcggcacgt ctgggttttg
     4381 catgtactga tacaaaagga atttgatcca gctcaagtag ttctgaagag gaaaagaatg
     4441 tcatttcacg gggataatga aacagagagt tgaccaaaca acctttttca agaatcaaat
     4501 agtccagcct tgccttttga gcttccagag cgcatgccag ccctattggt ccgcctccga
     4561 caatgataac gtcaagtata ttttgcaaaa taatctgtgt ttttaatatt taaattcgtt
     4621 ttttcataaa aagttatgct tacgattcag gtttttgcat attttagtga agaatttaaa
     4681 aaaataaagt aaatatgtca caatccaagc atccggccgg gcttaaggtc atgtt
//
LOCUS       DAOQQN010000257         4699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8444_length_4699_cov_4.778639, whole genome shotgun sequence.
ACCESSION   DAOQQN010000257 DAOQQN010000000
VERSION     DAOQQN010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8444_length_4699_cov_4.778639"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PLG24_09620"
     CDS             <1..485
                     /locus_tag="PLG24_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HMZ25191.1"
                     /translation="AALRGDWGEVVIEDGCNVQENCTLHMFPGKTIRLRKNAHIGHGA
                     VIHGADIGENCLVGMNSVVMDQVIVGAGSIIGALTFVHEGMEIPERSLVVGNPARIVK
                     QVSDTMLDWKKKGTRLYQSLPNDLRQYWAEVKPLTKIPDDRPSQEILFENWENIKLDK
                     "
     gene            complement(631..1236)
                     /locus_tag="PLG24_09625"
     CDS             complement(631..1236)
                     /locus_tag="PLG24_09625"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HMZ25192.1"
                     /translation="MKLLKFLLSICFLFFFLSSYAQTTINPRIGLNISHLSGKPSIGE
                     NESRTGFNIGFNLRFGDRFQFETGAHYATHGVGFNDARGKDTLDRAPMHFVKLPLLAN
                     INIVNSEVFRLRVYGGWMNNFLLKVDNNSLLSKEDVNVYTGGIHFGTGLDLTGLTIDL
                     SYEVGMMDFFKGNGLPLVLYPKNTQNNVLTLSVGVQVGGKR"
     gene            1453..2565
                     /locus_tag="PLG24_09630"
     CDS             1453..2565
                     /locus_tag="PLG24_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HMZ25193.1"
                     /translation="MKILDILKQEFMENTIGGYLWFVICILAALALKRVIANILINLS
                     IKLLSRHLSSINPEQFKKKLVKPLSFLILVSSIYLAVQYIDFPASWELADSKHFGIRK
                     VIVVLYGLMFGISLTWILIRLADCIGIILEKNYRPQDEKSTALQVIPFATDFIKIVIG
                     VVAGLIILSTLFGINVGTLVAGLGIGGLAVALAAKDTFENLLGSFVIYLDKPFEIGDY
                     IRINEIYCTVEKIGLRSTRLRTLEKSLLTMPNKMLVESMIDNYTLRPFRRVDRIIALS
                     RDTMHEQMITIIAGLKKAIGEHPLTTDEIYINFHEITSFSLEIRIQYYVMTAEWEIYL
                     KAVGEINLIILKIIEDNGSSLANVQGFLAFRNKATD"
     gene            3335..>4699
                     /locus_tag="PLG24_09635"
     CDS             3335..>4699
                     /locus_tag="PLG24_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMZ25194.1"
                     /translation="MADFSFITNAHPNVIEQLYNDYLKDPESIDKEWKAFFAGFDYSL
                     KYDGLSRPATTVPEDLSKEIGVSNLINAFRLRGHLLSSTNPIRRRKNRAPHLDLNDYG
                     LSEADMNRHFRAGDILGLNNATLKEIIQHLKNIYCSNIGFEFAHIENWERRNWLQQKI
                     ENRTPLQDYGLSNDRKHRILEKLNGAVMFEEFLHTKFVGQKRFSLEGGETTIPALDAM
                     VDFGTDRGVEEVIIGMAHRGRLNVLANIMGKTYEQIFTEFEGTVLPDQSFGDGDVKYH
                     LGYSSQVKNSSGREVYLKLVPNPSHLEAVNPVVEGFARAKADIMYKSDYDKILPILIH
                     GDAAAAGQGIVYEVVQMSQLEGYYTGGTLHFVINNQIGFTTDFEDGRSSTYCTASANL
                     VHAPVFHVNGDDPEAVVFAVELAVEYRQLFNSDVYIDMVCYRKHGHNEGDDPKFTQPQ
                     MYDII"
BASE COUNT         1513 a          994 c          931 g         1261 t
ORIGIN      
        1 gtgccgccct gaggggagat tggggagaag tcgtcattga agatggttgc aatgttcagg
       61 aaaactgtac tttgcacatg tttcccggga aaaccataag actcagaaaa aatgcacaca
      121 tcgggcatgg agcagtcatc catggagccg atatcggtga aaattgcctg gtgggtatga
      181 acagcgtcgt catggaccag gttatagtgg gggccggttc gatcatcggg gcattgactt
      241 tcgtgcatga aggaatggaa ataccggaaa gatccttagt ggtaggcaat cctgcacgaa
      301 tcgttaaaca ggtatccgat accatgcttg actggaaaaa aaagggaaca cgcttgtatc
      361 aaagcctgcc caatgacctc aggcaatatt gggccgaagt aaaaccccta acgaaaatac
      421 ctgacgacag accttcgcag gagatattat tcgaaaattg ggaaaatatc aagttggata
      481 agtagaggtc aacaagaaac agccctttac cggaacaaac tctaataagt ttccatctag
      541 gcaaatgttt tttgggaata tccaaagccg gtttttcccc tcaactcata atcacaacaa
      601 tatacacgta agcgaaaact ccggcgtatt ttatcttttt cctccaacct gtaccccgac
      661 actgagtgtc aacacattat tctgggtatt cttgggatat agtacaagcg gcaatccatt
      721 tcccttgaaa aagtccatca ttcccacttc ataggaaaga tcaattgtca atcctgtcag
      781 atcaagaccg gtcccgaagt gaattcctcc cgtatacaca ttgacatctt cctttgaaag
      841 aagagagtta ttatccacct tcaacagaaa attgttcatc catccaccat aaacccgcag
      901 cctgaaaact tcactattta cgatattaat attggccaaa agtggtaatt taacaaaatg
      961 catgggtgcc ctgtcaagtg tatccttacc tctggcgtca ttgaatccaa caccatgtgt
     1021 tgcataatga gcccctgttt cgaactgaaa acgatcacca aatcgcagat taaagccgat
     1081 attgaatccg gtccttgact cattttctcc aatggaaggc tttccggaca agtgacttat
     1141 attaagccca atgcggggat tgatggttgt ctgagcataa gaactcagga aaaaaaacag
     1201 gaaacaaata ctcaacaaga atttcaataa cttcatttct ttcaatttac tatactgcaa
     1261 atatatcaac tattttcaag agtctgaaaa aaggctgaga gacagtatcg aaataatcac
     1321 attacagaat ataccaggtc cattgattcg acagcctttg gccaataaat aaagttctcc
     1381 gagtatatca ttcacaacaa taatacatat ttttgaccat aaatatcaag ggaagttaat
     1441 ttcggtatag gtatgaaaat attggacatt ttgaaacagg aattcatgga aaacaccatt
     1501 ggtggctacc tatggtttgt aatatgtatt cttgctgcct tagccctgaa gagggtgatc
     1561 gccaatattc tgatcaatct ctccataaaa ttactgagca ggcatctctc ttccattaat
     1621 cctgaacaat tcaaaaaaaa actggtcaag cctctcagct ttcttatctt ggtaagcagt
     1681 atttacctag ctgtacaata tattgatttt cctgcttcct gggaattggc ggatagcaaa
     1741 cattttggta tccgtaaggt tattgtcgtt ctatatggtc ttatgtttgg gatatcactt
     1801 acatggattc tcatccggct ggcagattgt ataggaatca tccttgaaaa aaattaccgc
     1861 ccgcaggacg aaaagtccac agccttacag gtgataccat ttgctactga ttttatcaaa
     1921 atcgtcatcg gagttgttgc cggtctgatc attctcagta ctttatttgg aatcaatgtt
     1981 ggtacactgg ttgccggtct gggcattgga ggcctcgccg tagccttggc tgcaaaggat
     2041 acttttgaaa acctccttgg ttcatttgtg atctatctgg acaagccatt cgaaatagga
     2101 gattacatca ggattaatga aatttactgt acggtggaaa aaatcggact acggagcaca
     2161 aggcttagga cactggaaaa aagcctgctc acaatgccga ataaaatgct cgttgaaagc
     2221 atgattgata attacacgct cagacctttc aggagggttg accgcatcat agctctatcc
     2281 agagacacta tgcatgagca gatgattacc atcatcgccg gcctaaaaaa agcgattggc
     2341 gagcacccgc ttacaactga cgaaatctat atcaattttc atgagattac ttccttctcc
     2401 cttgaaatcc gcattcaata ttatgtgatg accgctgagt gggaaatata tctgaaagcc
     2461 gtaggagaaa ttaacttaat cattttgaag atcattgaag acaatggatc ttctctggcc
     2521 aatgtacaag gcttcttagc tttccgcaac aaggcgacag attaaacctc attccacggt
     2581 agtaacaacc ctttccaaat ccatactttg aattgcattt cagaagtatt gatcaattga
     2641 tgttgagaaa agttgaagat gttcggaata ttcaaaaatc acaacaaatt caataacaaa
     2701 aaaactaagc aatttatact accaaccatt ggtgattcac ctaccaacag aaggttagca
     2761 gacttaatga acaagcaaca ttatacgaac agaaattatt acaagaggac aacggtttct
     2821 gtcagggcat taaaattcat aataccaagc ctcccattga aggttaacct tacaaagtga
     2881 aaaaaatgaa aaacagaccc ccgaaattca atacaaactc tttccccaat gtttctgaga
     2941 ccagacaatt gagccaaaat taatattaat gaatctttaa tttttagaca aatctaaaat
     3001 atagaacagc cacgaataaa gccgcgaata tgcgtgaagc attttgacaa atacctaatt
     3061 attatattca tatataacta tatatcttgc tattaacaca ataatcaatt gtaaaataag
     3121 ttatagaaaa ggttaaaatt ccaataattg tcacttggtt tttgtcaaat ttcagataac
     3181 atttatcaag agaaacagat aaaaagccct atttaacatt gggcatacat gttatagccg
     3241 ttcaaacaag aaaaatgcca ataatatttg ccatatttgc aacgctcatc atgtaacagg
     3301 ttaattaaaa aattataatt atttagctat cgtcatggca gatttttcat tcattaccaa
     3361 tgctcacccc aatgtcattg aacagctata caatgactat cttaaggacc cggaatcaat
     3421 cgacaaggag tggaaagcat ttttcgcagg atttgactat tccctcaaat atgatgggct
     3481 gtccaggcct gccacaactg tcccggaaga tctttccaag gaaattggtg tcagcaatct
     3541 catcaatgcc ttcaggttga gaggccacct tctttcgtct accaatccaa ttcgtcgcag
     3601 gaagaacaga gcaccccacc tcgacctgaa cgactatgga ctcagtgagg cggatatgaa
     3661 cagacacttc cgcgccggtg acatattagg tttgaataat gccacattga aggagattat
     3721 ccagcatctt aagaatatat actgtagcaa tatcggcttc gaatttgccc atattgagaa
     3781 ttgggaaaga agaaactggc tgcaacaaaa aatcgaaaac cgcacacccc ttcaggatta
     3841 cggattgagc aacgacagga agcatcgcat ccttgaaaag ctcaatgggg ccgttatgtt
     3901 tgaagaattt ctacatacaa aattcgttgg tcagaaaaga ttctccctgg aaggaggtga
     3961 aacaaccata ccggcattag atgcaatggt agatttcgga accgaccgtg gtgtagaaga
     4021 agtcatcatc ggcatggcac atcggggcag actcaatgtc ctggcaaata tcatgggtaa
     4081 gacctatgag cagatattta ccgaatttga aggtaccgta ttacctgatc agagtttcgg
     4141 cgatggtgat gtgaaatacc atctcggcta ttccagtcaa gttaaaaatt catccgggag
     4201 agaagtctat ctcaagctcg tgccaaatcc gtctcactta gaagcggtca atcctgtcgt
     4261 tgagggattt gcaagagcta aggcagacat tatgtacaag agcgactacg acaaaatcct
     4321 gccgatcctg atccacggcg atgccgcagc ggcaggccag ggcattgtat atgaagtggt
     4381 ccagatgagt cagctcgaag gttattacac cggaggaacc cttcatttcg tgatcaacaa
     4441 ccagattgga tttactacgg atttcgaaga tggtcgttca tcgacttatt gcacagcatc
     4501 cgccaatctg gtccacgctc ctgtcttcca tgtcaacggt gacgatccgg aagcagtcgt
     4561 gtttgcagta gaactcgccg tcgaataccg acaattgttc aacagcgatg tatatattga
     4621 tatggtgtgt tacaggaagc acggtcataa tgagggcgac gatcctaaat ttacccagcc
     4681 tcagatgtac gacatcatc
//
LOCUS       DAOQQN010000258         4693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8452_length_4693_cov_6.465071, whole genome shotgun sequence.
ACCESSION   DAOQQN010000258 DAOQQN010000000
VERSION     DAOQQN010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8452_length_4693_cov_6.465071"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3502
                     /gene="dnaE"
                     /locus_tag="PLG24_09640"
     CDS             <1..3502
                     /gene="dnaE"
                     /locus_tag="PLG24_09640"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HMZ25195.1"
                     /translation="YKKAVADKMPGIAITDHGNMFGVFKFVSEAAKYNVKGQDPVIKP
                     IVGCEFYLVEDRFKRQFTKTLKDKRYHQLMIAKNEVGYKNLCKLSSLGYIEGLYGKYP
                     RIDKKLVEQYHEGIIATSCCLGAIIPQLILEDKIEEAEEELKWWVDLFDEDYYIELQR
                     HKIPDQEKVNQVLLRFARKYNLKVIASNDAHYVDREDSNAHDILLCVNTGEKQATPKS
                     DGFNVDESDGRGTRFGFYNDEFYFKTTDEMTHLFHDVPEAIENTLDIVEKVELLDLNK
                     NILLPHYTIPEKFKTQDEYLRHITYLGAKERYKELTPEVIERLDFELGVIENMGFAGY
                     FLIVADFIRAGRDIGVFVGPGRGSAAGSAVAYCIGITNIDPIKYNLLFERFLNPDRKS
                     MPDIDTDFDDEGRQKVIDYVIEKYGKNQVAQIITYGTMAAKSSIKDVARVLDLPLPQS
                     NELTKMFPEKLGISLNRVLKAPLTGENSLEEHDKLSTDDMAKVNELRQIFYNGTDLRS
                     MVLLEALKLEGSVRNVGVHAAGVIIAPTNLMDILPVATAKDSELLVTQYDGKVVESAG
                     VIKMDFLGLKTLSIIKDCLQYIKENHKVEIDIDAIPLDDGPTFELFQRGDTFGTFQFE
                     SAGMRKYLKELKPDRLEDLISMNALYRPGPMDYIPSYINRKHGLEEVTYDIDEMKGIL
                     EETYGITVYQEQVMLLAQQLAGFSKGDADGLRKAMGKKDRKTLDSLKGKFMEGIRARN
                     FNEAKANKVWTDWEKFAAYAFNKSHSTCYAFIAYQTAYLKANYLPEFMAANLKHQTNI
                     EKITQYIEQCKASDINVLGPDVNESKIAFAVNARGDIRFGLSSIKGIGDGPSQDILDA
                     RGSEPFADIYDFVVRVNARSINKKTMEALVYSGAFDCFNFEREAYFSANDKDLTFIEQ
                     LIRYAAAMKDMQEGSTMSLFEDDLDQVITQPQPFKYQPWAINYKLGKEKETIGLYASG
                     HPLDAYKVELEHLCNYSLSQLDSIDKLETVVRTGGLITSAYHGFTKKGDPYGKIELID
                     FNGKYSFFVSKDLYHKSKSYLAEGNKLFIEAVYRPVFRGGRPEIVITSIRLLESVGDT
                     YFKSVTLTMPIEMLTKKTIELLHQFVSENHGVQKLKIVIVDEASKVVLHTLAHDGIAI
                     NQLLLDIAIQNGMEYQVEMN"
     gene            3579..3809
                     /locus_tag="PLG24_09645"
     CDS             3579..3809
                     /locus_tag="PLG24_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25196.1"
                     /translation="MKVFLLRLAIILLGIVIMMSDLAAQCPMCRLAAESNLQNGGTAA
                     RGLDAGILYLLAIPYLLVGTIGFIWWKNQKSK"
     gene            complement(3896..4432)
                     /locus_tag="PLG24_09650"
     CDS             complement(3896..4432)
                     /locus_tag="PLG24_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25197.1"
                     /translation="MKRILLSFAMMISLSFFASAQNFDGKNIDIAAGIGLGSPYTYGT
                     SLIPPIWAQADFGVADKISVGGMLGFTTSKYGFTSYNWRYTYTLIGARGNYHWGKHLS
                     SLPDKLDLYAGVNLGYYLVKVNYDGYTGVIADGVSNSVFFGGQVGGRWFFKDNLAAIA
                     ELGYGIAYLRVGLNFRLK"
BASE COUNT         1440 a          859 c         1067 g         1327 t
ORIGIN      
        1 atataaaaag gctgtggctg ataaaatgcc cgggatcgcc atcacggacc acggtaatat
       61 gttcggcgtc tttaagtttg tatcagaggc cgctaaatac aatgttaaag gtcaggatcc
      121 ggtgatcaag cctattgtag gctgtgaatt ttatttggtg gaagatcgct tcaaacgaca
      181 gtttaccaag acgttgaaag acaagcgata tcatcagcta atgattgcga agaacgaagt
      241 agggtacaaa aacctgtgta aattgtcctc tcttggttac attgaaggat tatacggcaa
      301 atatcccaga attgataaaa agctcgttga gcagtatcac gaaggaatta tcgcgacttc
      361 atgctgcctg ggtgccatca tccctcaatt gattctcgag gacaagattg aggaagctga
      421 ggaagaatta aagtggtggg ttgatctgtt cgacgaagat tattatatcg aattacaacg
      481 gcataagata ccggaccagg aaaaagtaaa tcaggtactc cttcggtttg ccaggaagta
      541 taacctgaaa gtcattgctt ccaacgatgc tcactatgtg gatcgggagg acagcaatgc
      601 ccacgatatc ctgttatgtg tgaataccgg tgaaaagcag gcaacaccca aatcagatgg
      661 tttcaatgtg gatgaaagtg atggcagggg caccagattt ggcttttata atgatgaatt
      721 ctatttcaaa acaacggatg aaatgactca tctttttcac gatgtgccgg aagcgattga
      781 aaatacactg gatattgtag aaaaggtcga attactggat ttaaataaaa atattctgct
      841 gcctcattat acaattcctg aaaaatttaa gacacaggac gaatatttga gacacattac
      901 ctatctggga gcaaaggaaa gatacaagga attgacaccc gaggttattg aaaggcttga
      961 ctttgaactt ggcgtaattg aaaacatggg ctttgcgggt tacttcctta tcgtagcaga
     1021 ttttatcagg gctgggaggg atattggtgt ctttgtaggt ccgggacggg gttcagctgc
     1081 cggaagtgcg gtcgcttatt gcatcggtat taccaatatt gatccgatta agtacaatct
     1141 gctctttgag cgattcctga atccggatag gaagtcaatg ccggatattg atactgactt
     1201 tgatgacgaa ggaaggcaga aagtgattga ttatgtcatt gagaaatacg gtaaaaatca
     1261 ggttgcgcag atcatcacct atggtactat ggcagccaag agctccatta aggatgtcgc
     1321 cagagttttg gatttaccat tgcctcaatc gaatgagctg accaaaatgt tccctgaaaa
     1381 attgggcata tccttgaaca gggtactcaa agcaccattg acaggtgaga atagtcttga
     1441 agagcatgac aagctaagta cggatgatat ggcaaaggta aatgaactcc gccagatttt
     1501 ctataatgga accgatctgc gctcaatggt tttgctggaa gctctgaagc tggaaggttc
     1561 ggtgagaaat gtgggcgtcc atgcagcagg tgtaatcatc gcaccaacca acctcatgga
     1621 catccttccg gtagcaactg ccaaggattc agaactgttg gtgacacagt atgatggaaa
     1681 ggtagttgaa tctgccggag ttatcaaaat ggacttcctt gggcttaaaa ccttaagtat
     1741 catcaaagat tgcttgcagt acatcaagga aaatcataaa gtggagatag atattgacgc
     1801 catcccgctt gatgatggac caacctttga attgtttcag cgtggggata ctttcggtac
     1861 cttccagttt gaaagtgcag gtatgcggaa atatcttaag gagctaaaac ccgaccgatt
     1921 ggaggatttg atatctatga atgccttgta cagaccgggc ccgatggatt acataccttc
     1981 gtatatcaac cgtaaacacg gactggaaga agtaacttat gatattgacg agatgaaagg
     2041 tattctggaa gaaacctatg gcatcacagt atatcaggaa caggtaatgc ttcttgccca
     2101 gcaattagcg ggtttctcca aaggtgatgc agatggcttg cgcaaagcta tgggaaagaa
     2161 agataggaaa accctggact cattgaaagg taaattcatg gagggcataa gggcgagaaa
     2221 cttcaatgag gccaaagcca ataaagtgtg gactgactgg gagaaatttg cggcatacgc
     2281 attcaataag tcgcattcga cctgttatgc cttcatagca tatcagactg cctatctgaa
     2341 ggccaattac ctgccggagt ttatggcggc caaccttaag catcagacca acatagaaaa
     2401 gataacccag tatattgaac aatgcaaggc ttcagatatc aatgtgcttg gacctgacgt
     2461 caatgaaagt aaaattgcat ttgcagttaa tgccagagga gatatccgtt tcggtctcag
     2521 ttccataaag ggtattggcg acggcccgtc tcaggatatc ctggatgcaa gaggcagtga
     2581 accatttgcc gatatctatg attttgtcgt gcgtgtcaat gccaggtcca tcaataagaa
     2641 gaccatggaa gcactggtgt atagcggggc atttgattgc ttcaactttg aaagagaagc
     2701 atatttttct gccaacgaca aggatttgac cttcatcgag caattaatca ggtatgctgc
     2761 ggcaatgaaa gatatgcagg aaggaagcac aatgagtctt tttgaagatg atttggatca
     2821 ggtaattact caaccccaac cttttaagta tcaaccatgg gctatcaatt ataagcttgg
     2881 aaaggaaaag gagacaattg gcctttacgc cagcggtcat ccattggatg cttacaaggt
     2941 tgaattggag catctttgta actattccct gagtcaattg gattcgatcg acaagctgga
     3001 gacagtcgtt cggactggag gcctgattac atcagcttat cacggattta ctaaaaaagg
     3061 tgatccttat ggaaagattg agttgattga tttcaatgga aaatattcct tttttgtgtc
     3121 caaggatctt taccataagt caaaatctta tttggcggaa ggaaataaat tgtttattga
     3181 ggccgtttat aggccggtat tcagaggtgg tcgaccggaa attgtaatca cctccatccg
     3241 tcttttggaa tctgttggtg atacgtattt caaatctgta actttgacga tgccgattga
     3301 aatgctgacc aaaaagacta tagagttact gcatcaattt gtgtcagaaa atcacggtgt
     3361 ccaaaaatta aagatagtaa ttgtggatga agcgtctaaa gttgtactac acacactggc
     3421 tcacgatgga atcgccatca atcaattgtt gttggatatt gccattcaga atggaatgga
     3481 atatcaggtt gaaatgaatt agggttgcag gatattgttt ttctaatttg ctaaggtggt
     3541 tgcaaagtgt tacctttgta actcaaaata ctcctaagat gaaagtattc cttttgagat
     3601 tggcaataat attgttggga attgttatta tgatgtcaga tttggcagct caatgtccaa
     3661 tgtgccgttt ggcggcagaa tctaatcttc agaatggagg cacggcagcc agggggttgg
     3721 atgcgggcat attatacctg cttgccattc cttatttact ggtggggacg attggattta
     3781 tttggtggaa gaatcaaaag agtaaataac cattcttttc agattattat cacctaacat
     3841 gctaaaataa aaaagcccgg tttgcccggg ctttcttttt ttatagttgg atggattatt
     3901 tcaatctgaa attaagacca actctcaaat atgctatacc gtaacccaat tctgcaatag
     3961 ctgccagatt atctttgaag aaccaacgtc caccgacttg tccgccaaag aagactgagt
     4021 tggatactcc atctgcaata actccggtgt aaccgtcata gttaaccttt accaggtaat
     4081 agccaagatt gacacccgca tacaagtcca atttatccgg aagagatgaa aggtgcttgc
     4141 cccaatggta attacctcgt gccccaatca aggtataggt gtaacgccag ttataagaag
     4201 taaatccata cttggatgta gtgaaaccaa gcatacctcc tacgctgatt ttatcggcaa
     4261 cgccaaagtc agcctgcgcc catattggtg gaatcaaact cgttccatac gtgtagggag
     4321 agcccaagcc tatacctgca gctatatcga tgtttttgcc atcaaaattt tgagctgaag
     4381 cgaaaaaaga aagtgaaatc atcatagcga atgataaaag aattcttttc atgttttttt
     4441 gttttttaag tttaaataat aattgcacta aatattttgt cacttaatgt tttatacact
     4501 agctaaaagt actttggaat cctgatattt attatttatc atggtcgcct tatacagtaa
     4561 agtattagtg tatagaagcc tttcgcgact atatcactac aaagatatag aatttttaaa
     4621 tatattttat tggagcattt taattatttc aggattttac agaaacttac tttttacatg
     4681 tattctgtca ttt
//
LOCUS       DAOQQN010000259         4692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8458_length_4692_cov_5.006038, whole genome shotgun sequence.
ACCESSION   DAOQQN010000259 DAOQQN010000000
VERSION     DAOQQN010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8458_length_4692_cov_5.006038"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLG24_09655"
     CDS             complement(<1..446)
                     /locus_tag="PLG24_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HMZ25198.1"
                     /translation="MANISISRISQSRISEVDFDHLGFGKVFSDHMFVSDYRDGEWHD
                     HRIVPVEKISIHPSNMALHYGQAIFEGLKASKDKAGVPILFRPDKNIERFNRSAIRMC
                     MPEFPADLMLEALKQLVSLDRDWIPTFPGSSLYLRPFMFATDEFLGV"
     gene            668..997
                     /locus_tag="PLG24_09660"
     CDS             668..997
                     /locus_tag="PLG24_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25199.1"
                     /translation="MPKEEADNDIFINNIQKSTETLGLHFETEVKIIGLASDEKLNWT
                     GLNEFPTLKIAYFGESINPFRALKINQVAVLDNLSIFLTYSMSEIVKDPIKKGQFWNH
                     FKTFIQA"
     gene            994..1575
                     /gene="rdgB"
                     /locus_tag="PLG24_09665"
     CDS             994..1575
                     /gene="rdgB"
                     /locus_tag="PLG24_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HMZ25200.1"
                     /translation="MNIILLATANGHKLEEFSNILGKEYFIRSLKDYPELPEIEETGT
                     SLEENAGIKAKTLASHIGNTTIADDSGLIVPVLGGDPGIHSARYAGEHGNDSANRKLL
                     LQNLEDIHDRSAYFECVIALCNPDGDTLYFSGKLHGHIGFEEKGSNGFGYDSIFIPEG
                     SDRSLAEFSPEEKNQISHRRKAIDGLLAYLNKI"
     gene            complement(1675..2733)
                     /locus_tag="PLG24_09670"
     CDS             complement(1675..2733)
                     /locus_tag="PLG24_09670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ25201.1"
                     /translation="MTKINKLLIVGQGLAGTVFHFTLFQRGIHADIIDCDSPGKASSA
                     SAGIINPITGPKYNKSWNFDGLKNVFEPFYESLNSFLGTKVFNPMRMYRKLGDVQQVN
                     RWVYQSEQEVNNTCYGSFSTIPPEYGNDAPGEAWAEVHEAYRLDFRQVFTLYREKLRA
                     MGLFRGETFTYDSLTLEHDRPIYHGIEYDKVIFCEGYLVANNPYFNYLPVIPAKGDAL
                     IIEKSLPNSFMAKRTGMAVNWDGSHIWYGATLNRNFSSTEPDITDFKNLSSWYAEDFG
                     EIARVANHLSGIRPTSRDRRPLIGIHPDFPMLAILNGLGTKGTSLAPLMAKYLVENML
                     DGREIPNEVNINRVSPES"
     gene            complement(2717..3094)
                     /locus_tag="PLG24_09675"
     CDS             complement(2717..3094)
                     /locus_tag="PLG24_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HMZ25202.1"
                     /translation="MDIDIFREYCLSKPFVTEECPFGPDTLVYKVLGKIFALTGLDEV
                     ELSLNLKCDPEYAIELRERYASIIPGYHMNKTHWNTVHTGALNNDKLLKELIDHSYLE
                     VVKKLPKKLQAAFKNPGHNDENK"
     gene            complement(3106..3711)
                     /locus_tag="PLG24_09680"
     CDS             complement(3106..3711)
                     /locus_tag="PLG24_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HMZ25203.1"
                     /translation="MAGNNPPTLKFALLKYAGGGDWYNDVNSLHNLAQFCNQKMGTNF
                     DLDYATVEPSSAELFAYPITYMTGHGNVVFSDADAQNLRKYLIGGGFLIINDDYGMDP
                     FIRPALKKVFPELSLMEIPLNHPVYTSLFEFKGGLPKIHEHDGKPAQAYGLFWEGRLV
                     ALYIFETDLGDGWDDVHNDPFELRLKALQMGANIVKYAFER"
     gene            complement(3906..4529)
                     /locus_tag="PLG24_09685"
     CDS             complement(3906..4529)
                     /locus_tag="PLG24_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HMZ25204.1"
                     /translation="MNEAIIAGAISGMILGTLPGGVVFAVLQQSIEQGWRKGVGIATG
                     VVASDFMLILFVNLSTHGLAFVNKYDAYISMAGVALLLILGIGNLFIKSSLPISSRST
                     KGGAFYLFSTGFLLNTLNPVNFFMWMTITTQIQTRDFFNFTNMIWYFGTTLTMIFTCL
                     TMISFFSNRLRKWMTPEHLRYLNIVIGIIFIGLAIKLGYDAYMKHFF"
BASE COUNT         1478 a         1000 c          884 g         1330 t
ORIGIN      
        1 actcctaaaa attcatcagt tgcaaacatg aaaggcctta aataaagcga agaacccggg
       61 aaagtaggaa tccaatccct atccagggat acgagctgct tcaaagcttc aagcatcaga
      121 tcagccggaa attccggcat acacatacgg atagccgaac gattaaaacg ctcaatattc
      181 ttatccggtc ggaaaagaat aggcacaccg gctttatcct tacttgcttt caacccttca
      241 aatatagcct gaccataatg taatgccata ttagaaggat ggattgaaat cttttcaaca
      301 ggaactatcc tgtgatcatg ccattcacca tccctgtaat cagagacgaa catatgatct
      361 gaaaacacct ttccaaatcc taaatgatcg aaatcaactt cggaaattct actttgggag
      421 attctgctta tgcttatatt tgccatgtga aaaagggtta aacacaaaag tataaaattt
      481 ttaccaaaat atctaactat ttttttacat attaattagc ttatatttgt tattattgtt
      541 aattatttta taaacgaaga attatatttt gtagatgctg ctatctccta ttaaaatata
      601 caaaacacaa aggaaaaccg gggcaatatt ttttcaacat cctgatcaaa taaagttatt
      661 gattgtgatg ccgaaggagg aagcggacaa cgatatcttt atcaacaaca tccaaaaaag
      721 tacagaaaca cttgggcttc acttcgaaac agaagtcaag atcattggcc ttgcttctga
      781 tgaaaagtta aactggacgg gattgaatga attccctact ttaaaaatag catatttcgg
      841 tgagagcatt aacccgtttc gggctttaaa aatcaatcag gttgcagttc tagataattt
      901 aagcatcttt ctcacctatt ccatgtcaga aatcgttaaa gatccgatca agaaaggtca
      961 gttttggaat catttcaaaa ccttcattca agcatgaata taatcttatt agcaacggct
     1021 aacgggcata aacttgaaga attttcaaac attcttggca aggaatattt cattcgatct
     1081 ctaaaagatt atccggagct ccctgaaatt gaggaaacag gtacatccct ggaagaaaat
     1141 gcaggcatca aggcaaagac gttggcatca cacatcggca atactacaat agcagatgat
     1201 tcgggactta tcgttccggt acttggaggg gatcccggca tccacagcgc ccgttacgca
     1261 ggagagcatg gcaatgattc tgccaacagg aaattattat tgcaaaatct tgaggacatc
     1321 catgaccgat cagcatattt tgaatgtgtc atagccttat gcaatcctga tggagacacc
     1381 ctgtatttca gtggcaagct gcatggacat atcggttttg aggagaaagg aagtaatggc
     1441 ttcggttatg actccatatt tattccggaa ggttcagaca ggtccctggc ggaattttca
     1501 cctgaagaaa agaatcaaat cagtcaccga cgtaaggcta tcgatggcct attggcctat
     1561 ttaaacaaaa tataatccaa cgaactcaat tcattgtatt tctgtatttt atatatatca
     1621 tcttacaaat ttactgcaag ttattttcct gtgaaatgat ttcttcaaat catttcagct
     1681 ttccggactg actctattaa tatttacttc attgggaatt tctcttccat caagcatatt
     1741 ttcaaccaga tatttagcca ttaaaggagc caaagaagtt ccttttgtac ccaaaccatt
     1801 aagaatagcc aacatgggaa aatccggatg tatacctatc aacgggcgtc tgtcccggga
     1861 tgtaggccga atgcctgaaa gatgattggc cacccgggct atttctccaa aatcttccgc
     1921 ataccaggag gataaattct tgaagtctgt aatatcaggc tcggttgaag aaaaattcct
     1981 atttagtgta gcgccatacc aaatatggct cccatcccaa ttgacagcca tcccggtcct
     2041 tttcgccata aaactatttg gcaaggactt ttcaataatc agtgcatccc cttttgcagg
     2101 aataacaggt aaataattga agtaaggatt attggcaaca aggtagcctt cacaaaaaat
     2161 gactttatca tactctatac catggtatat tggcctatca tgttcaagag taaggctatc
     2221 ataggtgaag gtctctcccc ggaaaagtcc cattgcccga agcttttctc tataaagcgt
     2281 aaaaacctgc ctaaaatcca gacgatatgc ttcgtgtact tcagcccaag cctccccggg
     2341 ggcatcattc ccatactccg gcggtatagt ggagaatgag ccataacatg tattgtttac
     2401 ctcttgctca ctttgatata cccagcgatt gacctgttgc acatcaccca actttcggta
     2461 catgcgcatt ggattgaaaa cttttgtacc taaaaatgaa ttcaaggact catagaatgg
     2521 ttcgaagaca ttctttaaac catcaaaatt ccaactctta ttgtatttcg gtcctgtaat
     2581 cggattgatg atacctgcag atgctgatga tgcttttccc ggcgaatcgc aatcgatgat
     2641 atcagcatgg attcctcgct gaaacaaagt aaaatgaaac acagtaccgg ccaatccttg
     2701 cccgacaatc agcaacttat ttattttcgt cattgtgtcc aggattttta aatgctgcct
     2761 gcagcttttt aggcaatttt ttcactacct caaggtaaga gtgatcgatc aattccttca
     2821 gtagcttatc attgtttaat gctcctgtat gaacagtatt ccaatgtgtc ttattcatat
     2881 ggtaccccgg aataatggaa gcatatcgct ctctcaattc aatagcatat tcagggtcac
     2941 acttcaggtt aagacttagt tcaacctcat caagccctgt aagtgcgaaa attttaccca
     3001 aaactttgta taccagtgta tccggaccaa atggacattc ttctgtcaca aatggtttac
     3061 ttagacaata ttccctgaat atatcaatgt ccattgaaca ggtaattaac gctcaaaagc
     3121 gtatttgaca atattggctc ccatctgcaa agctttgagt cttagttcaa acgggtcatt
     3181 gtgtacatca tcccatccat cgcctagatc cgtctcaaaa atataaaggg ccacaagtct
     3241 tccttcccag aaaagcccgt aagcctgagc aggtttacca tcatgttcgt gtatcttcgg
     3301 caatcctcct ttgaattcaa aaagtgaagt atagacagga tgattaagcg ggatctccat
     3361 gagtgacaat tccggaaaga ccttcttcag tgcaggccga atgaaggggt ccatgccgta
     3421 atcatcgttg atgatcaaga aacctcctcc tatcagatat ttccgcaaat tttgagcatc
     3481 ggcatcagaa aagaccacat taccatggcc tgtcatataa gtaatgggat aagcaaataa
     3541 ttcagcactt gaaggctcta cggtagcata atcaagatca aaatttgtac ccattttctg
     3601 gttgcagaat tgagccagat tatgcaggct gttgacatca ttgtaccagt cacctccgcc
     3661 ggcatatttc agcaatgcga atttcagtgt tggaggattg ttgcctgcca tcgattgaaa
     3721 cggcaatgtt aataaaaata aatagctaat aaaactcttc atatggtaat ataacgccgt
     3781 aaaaggaatt gtgttataaa aatcgtattt acgaccggac aaagataatg gttttgaatg
     3841 gcaaagcggc aatcagcatt acaagtatat tattcaaaga agaattcaat agtgaatacc
     3901 ggaaactaaa agaagtgttt catatatgcg tcatagccca attttatcgc caacccgata
     3961 aaaataatcc cgatgacaat attcagataa cgcaaatgct caggtgtcat ccatttacgc
     4021 aatctgttgg aaaaaaagct gatcatggta agacaggtaa atatcatggt caaggtagtc
     4081 ccaaagtacc aaatcatatt cgtgaaattg aagaaatcac gtgtctggat ctgtgttgtg
     4141 atggtcatcc acatgaagaa attaaccgga ttaagtgtat taagcaggaa gcctgttgaa
     4201 aaaagataga atgctccgcc cttggttgaa cgagatgaga taggcaggct gcttttgatg
     4261 aaaaggttcc ctataccgag tatcagcaac agtgcaactc cggccatgct gatgtaagca
     4321 tcgtatttgt tgacaaaagc cagtccatgt gtgctcaggt tgacaaagag aatgagcatg
     4381 aagtcactgg ctactacccc tgtggcgatt ccgacgcctt tacgccaacc ttgttcaatg
     4441 ctttgctgga gcacggcaaa tacgacgcct ccgggcaagg tccccaatat catgccagaa
     4501 atggcacctg caatgatggc ttcattcaat tcccgggttc tttatctgct gaaagatcac
     4561 ttgacaatac tccaggtaac accatccttg ccgtccttaa cctgaatgtc caaccttgac
     4621 aatttatcgc gaataaggtc agatgtaggc caatccttat tttctctggc tttggccctc
     4681 agttcaagaa ta
//
LOCUS       DAOQQN010000260         4690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8465_length_4690_cov_5.889320, whole genome shotgun sequence.
ACCESSION   DAOQQN010000260 DAOQQN010000000
VERSION     DAOQQN010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8465_length_4690_cov_5.889320"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1412
                     /locus_tag="PLG24_09690"
     CDS             129..1412
                     /locus_tag="PLG24_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HMZ25205.1"
                     /translation="MTKGLAIEASAQYKADINNYIQTYKDIAIGEMERTGIPASIKLA
                     QGILESGAGKSELAVNANNHFGIKCGKDWLGKTYSREDDDYDTTGILIQSCFRAYTDA
                     TESYIAHSEFLRNPAKVSRYGFLFNIDPRDYKEWAIGLKRAGYATNPNYANLLIKTIE
                     DYKLYEFDQVSVFDLASNDKSDNIQATTGSPTGTKAKRSINRLSTINDVRVVMVNDGS
                     TIEELSQQYDVSVHALMKYNKEIKSKSETLAEKSMIFLQPRRNSYRGKNKWHYVKKGE
                     NIESIAKHYAIKETHLLRKNNLKKGEEPAIGEKLALRGWSIFNKKPILRSEKENILPP
                     KPTKPNNQDQDHLTGTAPVSNAVATTAKPATNGVATNQTKKPADDSRWTYHQIEKGDT
                     LYNLSKKYNTTVDHIKSLNNLGDEGIKIGQTIRVQ"
     gene            complement(2004..4595)
                     /locus_tag="PLG24_09695"
     CDS             complement(2004..4595)
                     /locus_tag="PLG24_09695"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HMZ25206.1"
                     /translation="MKLSTFRLLLLIELAISSTSLLNAQAPYFTSVALESREKPLPIT
                     EHLKKYALYQINIEDLRAHLLPAPLDVTKRSNTGILLDIPMPDGSVETFMMYEDPLLA
                     PEVAKKHPEIKTYTGYGIKHKSYTIKINFTIRGLSAVIVGLQKGEVFFEKQNNTETPV
                     YVSYYSDEVIYTENKGTSKYHSRCGTMEHEKQDGNIEPREKPKQRLSESFTNGTNLRT
                     IRLAMAADAEFTANRGGTKALAFAALTDYVNTLDAVYKYELSVKFTLVSGDNIVYTDP
                     NTDPYTNSDQVMMLDENQTNLDNVIGNSNYDVGHVLGYAGSSGGGIASSPSVCESAYK
                     GQGVSGVRDANDNNWPIVFDNQLIAHEVGHQFSMSHSYNSNIPVCTTREYNTSVEPGA
                     GTTIMSYGFTCDGDDYERPPYEPFLNFHVVSINQALTYLSNNPTCGTSSSTGNNIPVI
                     TVPGTSYTIPKSTPFSLSGTATDADGDDLSYSWEGTNISNEPDNNNLTTNTIYETDKP
                     PFFRSYKPLLASQNSQPGLRVFPRLEGILNGTNTAKGDKLPSIAITTTHTLSVRDNYG
                     GLATQDVTVTIDGTSGPFVITGDPSGTHNGNSSLTVTWDVSNTDNATINCTLVDIFLS
                     TDGGHTFPTELKHATANDGSESVTLPNINTSTARIKVAPSTPNIFFDISNQDFIISEV
                     LPVNLLSFEANLDKNQNVLLRWTTSNEVANKGFEVQISSDGTTFAAIDFVKRNENAAS
                     IKNYTYSVDRLSPGTYYFRLKQIDFSGSYTYSEIRTLTIKSHLNDPTLYPNPTKEKLM
                     LNPGTFNENLCKIEIADIAGNKVLSLPSKMYSDGSEINISSLPNGTYFLKISNGKEDY
                     LMNFIKY"
BASE COUNT         1513 a          890 c          877 g         1410 t
ORIGIN      
        1 atactaagat ttaaaagcag gatgtgacta tgcagttcca gaaaaaaaag tcaattggtc
       61 aacaaatatt caaaggaaca ccgaaagcaa tttatcggcg actcgtgctt ttgctataca
      121 ttgtattgat gactaaagga cttgcgattg aagcatcagc acaatacaag gcggatatca
      181 acaattatat ccagacctac aaagacatcg caatcgggga aatggaacga accggtattc
      241 cggcaagtat caagctggct caaggcattc tggagtccgg agccggcaaa agtgaacttg
      301 ccgtcaatgc caacaaccat tttggaataa aatgcgggaa agactggctg ggcaagacat
      361 acagccgcga ggacgatgat tatgacacga caggtatact gatacaaagt tgtttcagag
      421 cttatacaga tgccacagag agttatattg ctcactccga atttttgcga aatccggcta
      481 aagtatcccg atacggattt ttattcaata tagatccgag agattacaag gagtgggcga
      541 taggactgaa aagggcaggt tatgctacca atcccaacta tgccaatttg ctgatcaaaa
      601 caattgaaga ttataaattg tacgaatttg atcaagtatc agtctttgac ctggcatcca
      661 atgacaaaag tgataatata caagctacaa caggttcacc cacgggcact aaagccaaac
      721 gcagtatcaa cagactttcc accatcaatg atgtacgtgt ggtgatggtc aatgatggct
      781 caacaatcga ggagctaagc cagcaatacg atgtgtctgt ccatgcccta atgaaataca
      841 ataaggaaat caaatccaaa agcgagacgc tcgctgagaa atccatgatt tttcttcaac
      901 caagacgcaa tagttataga ggcaaaaata agtggcacta cgtcaagaaa ggagaaaata
      961 tagaatccat cgcaaaacat tatgcaatca aggaaactca tttattgcga aagaacaacc
     1021 taaaaaaggg tgaagaacct gccatcggtg aaaaacttgc ccttcgcgga tggtctattt
     1081 tcaataagaa acccatactg aggagtgaaa aagaaaatat ccttcctcca aagcccacca
     1141 agcccaacaa tcaggatcaa gaccatttaa ccggaacagc tcctgtatcc aatgcggtag
     1201 ctactactgc caaacccgcc acaaatggag ttgccacaaa tcagactaaa aagcctgcgg
     1261 atgatagtcg ctggacctac catcaaatag aaaaagggga cacattgtac aacttgtcaa
     1321 aaaaatacaa taccacagtc gaccatatca agtcactgaa taatctgggc gatgaaggta
     1381 ttaaaattgg ccagacaatc agggtacagt aaaagattag tttgaggaga tcattcaaga
     1441 tactcaatct gcctatccta tctgatgcta ataaattgca gcattccgga tacaaagtat
     1501 taactgacgg aaagactttg attgaaaatc cctctccggg ctatatattc tttataggtg
     1561 tattacaaat cgcaataaaa atgaattatt ttaacacttt ttgaaaatgg gtaaatacaa
     1621 taacgctccg caattcgata ggattgcatc cggaaaggta aaaagatata aatatcaaat
     1681 tgcctggtga aaaaatcaag tatcctcaag aagtaagttg tgcaagaaat tgacaccgtg
     1741 gaagaagggt aaagaattct acatatcctt gattcttgtg ttagagaaga gttattatat
     1801 agtacaaaat ttacggatac aacactgatt ttaataatag tggggattta aatatctttt
     1861 catcttaatt atttgaaagc ctgatttgat atatgcacct atatcacaag catagcccca
     1921 aaaaaacaaa ggaactgaaa tttcagttcc tttgttttta ggcttatata aatactacac
     1981 gcctttagaa ggaaaatgca aggttagtat tttataaaat tcatcaggta gtcttctttt
     2041 ccattgctta tttttaagaa ataggttcca ttgggcaaag atgaaatgtt tatctctgaa
     2101 ccatctgaat acattttcga aggaagactc agcaccttgt ttccggctat atcagcaatc
     2161 tctattttac aaagattttc attaaatgtt ccggggttta acataagttt ctctttagta
     2221 ggattaggat acagtgtagg atcattcaaa tgagatttta tcgtcaaggt tctaatttcc
     2281 gagtatgtat aagaacccga aaaatctatt tgttttagtc taaaatagta tgtaccgggt
     2341 gacaatcgat ctacagaata tgtgtaattt ttaatcgatg cggcattttc atttcttttt
     2401 acgaaatcta tagcagcaaa tgttgtccca tccgaactaa tttgtacttc gaagccttta
     2461 tttgcaactt cattggaagt ggtccatctc aatagtacat tctgattttt atccaagttg
     2521 gcttcaaatg aaagaagatt taccggaagg acctcgctaa taataaaatc ttggttcgag
     2581 atatcgaaaa agatattagg agtacttgga gctactttta tcctagctgt actggtattt
     2641 atattaggca atgtcacact ctcactacca tcatttgcag tagcgtgttt taattctgta
     2701 ggaaaagtat gtcctccatc agtagataga aagatatcaa ctaaagtaca gttaatagta
     2761 gcattatccg tattacttac atcccaggtt actgtcaaac tactgtttcc attatgtgtt
     2821 ccactaggat ctcctgttat gacaaacgga cctgatgtcc catcaatagt cactgtgaca
     2881 tcctgagtgg ccaatccacc atagttatct ctgacggaaa gagtgtgggt ggtagtaatg
     2941 gcaatagatg gcaatttatc acccttagct gtattagtac cgttcaggat accctctaac
     3001 ctaggaaaga ctctaaggcc tggctgagaa ttctgactgg ctaataaggg tttatacgat
     3061 ctaaaaaaag gaggtttatc cgtctcatag attgtatttg ttgttaaatt attattatca
     3121 ggctcattgc tgatgttcgt tccttcccaa gaataactaa gatcatcacc gtctgcatcc
     3181 gtggctgtgc ctgacaaact gaagggtgta gatttaggta tggtataaga ggtaccggga
     3241 actgtgatga caggtatatt attgccggta ctggaagaag ttccgcatgt agggttatta
     3301 cttaaatatg ttaatgcttg attaatactt actacatgaa aatttagaaa tggctcgtag
     3361 ggcggtctct catagtcatc cccatcacag gtaaatccat agctcatgat agtagtaccg
     3421 gcaccgggtt ctacagaagt attgtattct ctggtggtac atacgggaat attgctgtta
     3481 tagctatggc tcatgctaaa ctggtggccg acttcatgag cgattaactg attatcgaaa
     3541 actataggcc agttattgtc atttgcatct ctcaccccag acacaccctg acctttatat
     3601 gcgctttcac agactgaggg cgaagaagct atacctccac ctgaactacc tgcatatcct
     3661 aatacatgac ctacatcata attgctgttt cctattacat tatcgagatt cgtttggttc
     3721 tcatccagca tcattacttg atcactattg gtataggggt ccgtatttgg atcagtatat
     3781 actatattat caccacttac taaagtaaat tttacactta actcatattt atacactgca
     3841 tctaaggtat ttacataatc agttaatgca gcaaaagcca gcgctttagt ccctcctcta
     3901 ttagctgtaa actctgcatc ggcagccata gctagtctta tagtccttag attagtacca
     3961 tttgtaaaac tttctgataa tctttgcttt ggtttttctc tgggttcgat atttccatct
     4021 tgtttttcat gctccatagt accacatcta ctatgatact tagaagtacc tttattttct
     4081 gtataaatca cttcatccga ataataactt acatatacgg gtgtctctgt attattttgc
     4141 ttttcgaaaa atacctctcc tttttgtaag cctactatca cagcacttag tcctcggatg
     4201 gtaaaattga tttttatggt ataacttttg tgttttatac catagccggt atacgtcttt
     4261 atctcaggat gttttttcgc cacttcagga gccaatagcg gatcttcata catcataaag
     4321 gtctctactg atccatcagg cataggaata tctaaaagta tacctgtgtt actcctttta
     4381 gttacatcta agggagcagg taataaatgt gctcgtaaat cctctatatt tatttgatat
     4441 aaagcatact tttttaagtg ctctgtaata ggtaatggct tttctctgga ctccaaagct
     4501 acggaggtaa aataaggagc ctgagcattg agcaatgaag tactacttat ggctaattca
     4561 atgagcaata ataatctgaa tgtagataat ttcatggtaa tatattttaa ttgaatgtaa
     4621 cagtgaactg caaactattt tttgtaaata aatttttgtg ttaggaaata gacacttagt
     4681 gagcgtagct
//
LOCUS       DAOQQN010000261         4688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8472_length_4688_cov_6.881286, whole genome shotgun sequence.
ACCESSION   DAOQQN010000261 DAOQQN010000000
VERSION     DAOQQN010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8472_length_4688_cov_6.881286"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="PLG24_09700"
     CDS             <1..813
                     /locus_tag="PLG24_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HMZ25207.1"
                     /translation="VNDDLYKTISMSKVEKKIIKTTDGKDMVAWVIYPPNFDPNKKYP
                     TLLYCQGGPQSALTQFYSFRWNMQLMAANGYIVIAPNRRGMPGYGQEWNEAISGDWGG
                     QPIRDYLSAADAMAAEPYVDKNRMGAVGASYGGYSVYMLAGKHEKRFKTFISHCGLFD
                     MDSWYGSTEELWFANFDLKGPYWQKPQPKSYTDFNPIKFVDRWDTPILIFEGEKDYRV
                     PYTQGLEAYQAAQLRGIKSRLVIYPDEGHWVTKPFNSLVWHHEFFRWLRETL"
     gene            complement(1084..2346)
                     /locus_tag="PLG24_09705"
     CDS             complement(1084..2346)
                     /locus_tag="PLG24_09705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ25208.1"
                     /translation="MRDRIINSLLFLFIFYSAGVAQSISSSHAVMDAAVTGKIINRES
                     VLLGKKINLQTDQNTYPDIELNGDGTFAVELPENFPLRYRLFDDKDFLNGINVGDIIK
                     IHSYILGKNPINDPLSLIAADVNRSGKVTVADIVEIRRLILGKVDQFQSGDSWIFIDA
                     EFPLFINNWELANTYVNPTTDNSFYNEFLAVKVGDVDRIIFSRSDDVYIRDPSSKSIA
                     YLTRDQGRIMLWLDKTARHAEGIQLGLNLDFGLQFTSCNPMLDDDQYSFSEDGKSVKI
                     VLLNDEIEALYDKPLFIFDYYTLNNDQEPIRLSEDFENMIISNGESFSVAPIGFKKLS
                     VSASYPSPNPFRDNTVISFKSEVVQDVKLEVYDLNGCKIYNQNINALPGQNTVSLTRT
                     ALKERAGVYYYNLFGKDIHSQGRIIMLD"
     gene            complement(2907..3866)
                     /locus_tag="PLG24_09710"
     CDS             complement(2907..3866)
                     /locus_tag="PLG24_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25209.1"
                     /translation="MIPAIPGYSVEAWKSFVFNPGRLRRMQEGAGIILDFLQLVLGQG
                     LLASELLSEDNLKELSTRLVDTQIPGAARKIKSFARYELNPETLPMFRFELRYLGNLA
                     HMLMGFEQLSLSAKLQVWQICGGLISKELVLNQPGVNDRWVTKSVVVAREDGLVTRKT
                     WFYGTHTLMWAFTLDYSFGTQPLPEGNKVGDTFNGTAKFYPGLLPGRILFTEKAEDSG
                     VYRTPDVMRFEDIARLKLGVARLAAHDPLFHEYPVLMDKIILTSNNGQFYLVDVNNEF
                     LPLITVEKRGYGSKDDAWERFADAGGHPVSLWVMVSQGQFILI"
     gene            complement(3863..4540)
                     /gene="rnc"
                     /locus_tag="PLG24_09715"
     CDS             complement(3863..4540)
                     /gene="rnc"
                     /locus_tag="PLG24_09715"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HMZ25210.1"
                     /translation="MLGYTPAHLELYVQAFKHKSNSVEVNGHRQHNERLEFLGDAILN
                     SIVAEYLYKKYPNGDEGFLTKMRSKIVKRKTLDAIGARMGIDDLMTIMNKTQISRSML
                     GNALEALVGAVYLENGYAFTRKFIVLEILRKYLDIHRLEGMDDNFKSQLLEWCQKNNM
                     EVNYELVSKYKFEKRDRFKIAVMIQGEQVAEADDFNKKNAEQIASEKAMMKLGILSLE
                     DAETSLL"
BASE COUNT         1424 a         1044 c          840 g         1380 t
ORIGIN      
        1 gttaatgatg atttgtacaa gactatcagc atgagcaagg ttgaaaagaa gatcatcaag
       61 acaacggacg gaaaggacat ggtcgcctgg gttatttatc caccaaactt tgatcccaat
      121 aaaaaatacc caacattact gtactgccag ggaggtccac agagtgctct gacacaattc
      181 tattctttca gatggaacat gcagcttatg gcagccaacg gatacattgt catagctccg
      241 aaccgtcggg gaatgcctgg ttatggccag gaatggaatg aagccatttc aggtgattgg
      301 ggtggacaac cgattagaga ttatctaagt gcagctgatg ccatggctgc agagccgtat
      361 gttgataaaa acagaatggg agcagtaggc gcatcttacg gaggatattc tgtgtatatg
      421 ctcgccggaa agcatgaaaa acgattcaaa acctttatct cacattgtgg acttttcgat
      481 atggatagct ggtatggttc tacagaagaa ctatggtttg ccaacttcga cctgaagggt
      541 ccctattggc aaaaacctca gcccaagtca tataccgact tcaacccaat aaaattcgtg
      601 gaccgatggg atacaccaat attaatattt gaaggagaga aggactatcg cgtaccttat
      661 acccagggat tggaggctta tcaggctgca caactcagag gaattaaatc aagactggta
      721 atctatcccg atgaaggcca ttgggtcaca aaacccttca atagcctggt gtggcaccat
      781 gagttttttc gatggcttag agagactttg taatgcccac cttgataaag gtctgtaccc
      841 gatctgaata gctctggtaa agaacctgat actatcaacc gcaataaaaa agtaaagaga
      901 ctatccaaca aggcagatag tctcttttca ttttccggaa aatggtaaac agtagtagta
      961 tcccttctca ataaatgaat cgtataaaaa aaggccatcc ttttaagaca gcctcttttt
     1021 gtccaaattc ataataatta aaacctacct aatccgttgc ttccgaaacg ggtatttctt
     1081 caatcaatcc agcattataa tccttccctg gctatggatg tctttcccga aaagattgta
     1141 gtaatagact cccgctcttt ctttcaacgc tgttcgggtt aaagaaacag tgttctgtcc
     1201 gggcagagca ttgatatttt gattgtatat tttacatcca ttcagatcat agacctctag
     1261 tttcacatcc tgaacaactt ctgacttaaa actaattacc gtattatccc taaaaggatt
     1321 ggggctcgga taacttgctg aaacagaaag cttcttaaat ccaatcggtg caacggaaaa
     1381 ggattcgcca ttcgatatga tcatgttttc aaaatcttct gacagcctaa taggctcctg
     1441 atcattattc aatgtatagt aatcaaaaat aaatagcggc ttatcatata atgcctctat
     1501 ttcatcattt aatagcacta tcttaacact tttgccatct tcactgaaag aatactgatc
     1561 atcatcaagc attggattgc aggaagtaaa ttgcagtccg aaatccagat tcagccccaa
     1621 ttgaatacct tcagcatgcc ttgccgtttt atccaaccaa agcattatcc tcccctgatc
     1681 acgtgtcaga taagcgattg acttacttga aggatctctg atatagacat catcacttcg
     1741 actaaatata attctatcga catcgccaac ctttacagcc agaaattcat tgtaaaaact
     1801 attatctgta gtcggattaa cataggtatt ggccaactcc caattattaa tgaatagcgg
     1861 aaattctgca tcgataaata tccatgaatc tccggattga aattgatcta cctttcccaa
     1921 tatcaatctt cttatctcaa cgatatcggc aacggtcacc ttcccactcc tgtttacatc
     1981 tgcagcaatc aggcttaacg gatcgtttat tgggttctta cctaaaatgt aggaatgaat
     2041 cttgatgata tctcctacat taataccatt cagaaagtcc ttatcgtcaa acaacctata
     2101 ccgaagtgga aaattctccg gcagctcaac tgcaaacgtt ccatcaccat tcaattcaat
     2161 atcagggtaa gtattctggt cagtttgaag attgatcttc tttccaagta atacactctc
     2221 ccgattgatt attttaccgg tcactgctgc atccataact gcatgtgaag aggaaataga
     2281 ttgtgcaact cctgctgaat aaaaaataaa caggaataac aggctattta taattctatc
     2341 tctcatagtg aatatttcca atgcacgata gcagctattt ctaagattta tcaggaacaa
     2401 atgattttcc atatcagtaa gattaaaatc attgattttc tattgagaac gaatgaaaca
     2461 ctcaggcaat gccttcgaat tgttatttca tcagtctgta aaagatctat cttgactggt
     2521 ttgaggatgc caccttgacc agatctccga taggagaccg ataccaggcg ttaaattctt
     2581 ataccattgt gaccagtcat tacttgcacc gacccacaga tctaatacgg ccttaaccgt
     2641 attagaacca tggataaaat tcataccttt aaagataagc aacagagcag agtttccttt
     2701 gaaatctgat ggaactatta atgccccaaa agcatgaagt ggtaaagttt aaaaacctta
     2761 atacaaaaat caaacgagta aattctataa gacattactc cttgacgatc ccatccgttg
     2821 cctcaaattt gattttttat tttaatattt attgtgttaa tatatataaa tctgtaatag
     2881 tatttattat atattaaata ttttaattaa attaaaataa attgcccttg ggaaaccatc
     2941 acccacaggc tgaccggatg ccccccggca tcagcaaacc gctcccaggc atcatccttg
     3001 ctaccatatc ctcttttctc cacagttatc aatggcaaaa attcattatt cacatccact
     3061 aaataaaact gtccattatt gctggtcaat attatcttat ccatcaaaac cggatactca
     3121 tggaacaatg gatcatgggc agccaatctg gctactccga gtttcaacct tgcaatatct
     3181 tcaaaacgca tcacatcagg tgtccgatat acaccggaat cttctgcttt ctctgtaaaa
     3241 agtattcggc ccggtaacaa tcccggataa aatttggctg ttccgttaaa cgtatctcct
     3301 actttattcc cctcaggcag gggttgtgtc ccgaagctgt aatcaagagt aaaagcccac
     3361 atcaaggtat gcgtgccata gaaccatgtt tttcttgtga caaggccatc ttctcttgca
     3421 actacaacac tcttagtcac ccagcgatca ttaacaccgg gttgattcag aacaagttcc
     3481 ttactgatca gcccgccaca gatctgccaa acttgtagtt ttgctgacaa agataattgc
     3541 tcgaagccca tcaacatatg tgcgagattg cccaggtatc tcaactcaaa acgaaacata
     3601 ggcaatgtct ccggattgag ttcataacga gcgaatgact ttattttgcg agccgctccg
     3661 ggaatttgcg tatcgaccag tctggtggat aattctttca aattatcttc agaaagcaat
     3721 tcactcgcta aaagtccttg ccctaatact aattgaagaa aatccagtat gatgcctgct
     3781 ccctcctgca ttctacggag tcggccgggg ttaaaaacaa agctcttcca ggcttcaact
     3841 gaatatcccg ggatggcggg tatcacaaca ggctcgtttc agcatcctcc agtgagagga
     3901 tacctaattt catcattgct ttttcggatg caatctgctc ggcatttttc ttattgaaat
     3961 catctgcctc cgctacctgt tctccttgaa tcattacagc aatcttgaac cggtctcttt
     4021 tttcaaactt atatttggat accagctcat agttaacttc catgttgttt ttctgacacc
     4081 attcgaggag ttgactcttg aagttgtcat ccatcccctc cagacgatga atatccaggt
     4141 atttgcgtaa aatctcgagg acgataaact ttctggtgaa ggcataccca ttttccagat
     4201 aaacggcccc gacaagggct tcaagggcat tacccaacat agatctgctg atctgagtct
     4261 tattcatgat ggtcatgagg tcatcgatcc ccattctggc accaatggca tccagggtct
     4321 ttcgtttcac gattttcgag cgcattttgg taagaaaacc ctcgtctcca ttgggatact
     4381 tcttataaag atattcagct acaatgctgt tcaggatagc gtcgcccagg aattcaagcc
     4441 tttcgttatg ttgtctatgg ccattgactt caaccgaatt tgatttatgc ttgaatgctt
     4501 gaacatatag ttccaggtgg gcgggagtgt agcccagcat tgaagataac tttccacaaa
     4561 agtgtttgtc tttcgaaaaa taacggttaa aaaattttaa taagcctttc acagtttaat
     4621 gttaatcctc aaattttttg ataataatac aggcattgtg accaccaaag ccgaaagtat
     4681 tactcatc
//
LOCUS       DAOQQN010000262         4661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8541_length_4661_cov_5.927269, whole genome shotgun sequence.
ACCESSION   DAOQQN010000262 DAOQQN010000000
VERSION     DAOQQN010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8541_length_4661_cov_5.927269"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PLG24_09720"
     CDS             complement(<1..731)
                     /locus_tag="PLG24_09720"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HMZ25211.1"
                     /translation="MRIGFDAKRLFVNNTGLGNYSRTLVENLHRFHPEHDLYLYAPRL
                     VRNDRTEVFFESGYRLRSPMGPAKMGWRSFGMTHNLRKDGIELYHGLSHELPFNLEKS
                     KIPSVVTIHDLLYKYFPMDFPLIDRWVYEKKFSAACSKADRIIAISEATRTDIMEHFG
                     IRPQRIQVIYQTIHPIFRHTPAMETISNVREKYNLPAEFILYVGSIIRRKNLEVLIKA
                     LDLMPPKKRLPLIIVGNGKIYLDYI"
     gene            complement(715..1641)
                     /locus_tag="PLG24_09725"
     CDS             complement(715..1641)
                     /locus_tag="PLG24_09725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ25212.1"
                     /translation="MPSGNYSLSICIPVFRQDVRNLVNQLSEQVDNVNIPVEILIADD
                     GSPEEFMVINRDCANERNILFFGNTINVGRAAIRNFLARHSKGSHLLFLDGDSSIELS
                     DFLSKYIECINTNPDAVWCGGTYFPIHKADSVHSLHFRYGNQVVARLHSHQDKNGRFF
                     FMSSNFMIPSQVFIAIGFDENIRDYGHEDTLFGCRLEQNLIPVLSVQNAVMHDDLDTN
                     GVFIHKTEQSVRNLRSLISLPQYKDCLQKTSLVKSFLFLGKWRLAGLYRIFFGMIIPL
                     VLHNLRGAKPSLNLLQAYKLYLMVNHGHEDRV"
     gene            complement(1641..2822)
                     /locus_tag="PLG24_09730"
     CDS             complement(1641..2822)
                     /locus_tag="PLG24_09730"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HMZ25213.1"
                     /translation="MAKKAKSGASAPRSMKFNSKLLHAGIEPDPSTGAIMTPIFQTST
                     YVQEEPGVHKGYEYARTANPTRTVLEANLAALENGTFGLCFSSGLGAMDTILKLLSPG
                     DEIISTNDLYGGSYRLITRVYGRFGIKSHFIDLNDTAQLERLINPRTRLIWIETPTNP
                     MLNIVDIRKVTKVAEKYGIITCVDNTFASPYLQQPLDLGADIVLHSATKYLGGHSDVV
                     MGAVVCRKKEIEDQLRFLQNACGATPGPMDCFLVLRGIKTLHVRMKRACRNAARIAEF
                     LKTHPAVNRVNFPGFKTHPSHELAVAQMKDFGAMVSFDLKKDSTEEAKAVLRKFRMFS
                     LAESLGGVESLVGHPATMTHASIPREERIKVGLTDSLIRLSVGIEDFDDLKADLEQAL
                     K"
     gene            complement(2827..3786)
                     /locus_tag="PLG24_09735"
     CDS             complement(2827..3786)
                     /locus_tag="PLG24_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HMZ25214.1"
                     /translation="MNIHDIVATGKKATRDGYGEGIFEAGKRNKNVIAMVADLLGSLK
                     LEQFIEAFPDRFVQMGIGEANMMGVAAGLATAGKIPFTGTFANFSTGRVYDQIRQSIA
                     YSGKNVKICASHAGLTLGEDGATHQILEDIGLMRMLPGMTVICPIDANQTRQAVLALV
                     DHQGPAYLRFGRPDWPVFTDAETPFVIGKADRLGSGTDVSLFATGHMVWNAVEAAKSL
                     EARGVSVDVISVPTIKPLDADTILDSFSKTHCGVVCEEHQINGGLGDAIAHLVTQNKP
                     YPLEFVGVNDKFGESGKPLDLLKKYGLDAEDIVAAAHKVLKRK"
     gene            complement(3790..4647)
                     /locus_tag="PLG24_09740"
     CDS             complement(3790..4647)
                     /locus_tag="PLG24_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HMZ25215.1"
                     /translation="MIATQVRRDILRMVYIASSGHPGGSLGCAELLTALYFSVMDQSE
                     EFKMDARGEDAFVMSNGHISPLLYSVLARRGYFPLEELSTFRKLNSRLQGHPTTHEGL
                     PGIRVATGSLGQGFSVGVGIALAKKMDNDPGFVFVSTGDGELQEGQIWEAAMFAPFHK
                     LDNLIVIVDWNGQQIDGPNNEILSLGDLEGKWRTFGWRVVVTNGNDMESIVNALEEAK
                     THRGSEIPTVILMRTNMGEGVDFMMGTNKWHGKAPNKDQVEIALAQLEETLGDYVYNE
                     NYQAPLPKH"
BASE COUNT         1234 a         1159 c         1020 g         1248 t
ORIGIN      
        1 tctatataat ccaggtatat cttgccgttg cctacgatta tcaggggaag ccgctttttg
       61 ggaggcatca ggtccagcgc cttgatgagg acttccaggt tctttctcct gatgatggag
      121 ccgacataca gaataaattc agccgggaga ttgtattttt ccctgacatt tgaaatggtt
      181 tccatggcag gagtgtgcct gaaaatcgga tggatcgtct ggtaaatcac ctgaatcctt
      241 tgcggccgga taccaaaatg ctccataata tcagtccgtg tggcttcact gatggcaatg
      301 attcggtcag ctttcgaaca ggctgcgctg aatttttttt cataaaccca ccggtcaatt
      361 aacggaaaat ccatcggaaa atacttgtac agaagatcat ggatagtaac gaccgaaggg
      421 atttttgatt tttccaaatt gaatggcaat tcgtgactca agccatgata cagctctatc
      481 ccatcttttc taagattatg tgtcatacca aagcttcgcc agcccatttt agcgggaccc
      541 ataggagagc gcaaccgata acccgactca aagaacacct cagtccggtc atttctcacc
      601 agacgaggag catacagata gaggtcatgt tccggatgga atcggtgcaa attttccact
      661 aaggtccggc tatagttacc aaggccggta ttattgacaa acagtctttt agcatcaaac
      721 cctatcctca tgtccatgat ttaccatcaa atatagctta tatgcctgta aaaggttgag
      781 ggaaggctta gctccccgta aattatgtag gacaaggggt atgatcatgc cgaaaaagat
      841 gcgatacagt ccggccaacc tccattttcc aagaaaaagg aatgacttta ccagactggt
      901 tttctgcagg caatccttat actgcggcaa agaaatcaac gacctcaagt ttcgaacgga
      961 ttgttctgtc ttatggataa acaccccgtt cgtgtcaaga tcatcatgca tcactgcatt
     1021 ttgtactgaa agtacaggaa ttaagttctg ctccaaccgg cacccaaaca aggtgtcttc
     1081 gtgcccgtaa tctctgatgt tttcatcgaa acctatcgct atgaaaacct gacttggtat
     1141 catgaaattg gaagacatga aaaagaacct tccgtttttg tcttggtggg aatgcagtcg
     1201 ggcaaccact tgattgccat aacgaaaatg cagcgaatgt acagaatcgg ccttgtgtat
     1261 aggaaagtat gtacctccgc accatacagc atccgggttc gtgttgatac attcgatgta
     1321 tttgcttaag aaatccgaaa gttcaataga agaatcgcca tccaggaaca acaaatggct
     1381 tcctttgcta tgcctagcta aaaaattgcg gatagcagca cgacccacat tgattgtatt
     1441 tccaaaaaag agtatgttcc tttcattggc acaatcccgg ttaatcacca tgaattcctc
     1501 cggagaccca tcatctgcaa tcaatatttc aaccggaata ttcacattat ccacctgttc
     1561 tgacaattga ttcaccagat tccggacatc ttgccgaaac acgggaatac agattgacag
     1621 ggaataattt ccggacggca ttacttcaaa gcctgttcaa gatcggcttt cagatcatca
     1681 aagtcctcta tacccacact cagtctgatc agggagtctg ttagaccgac cttgatcctt
     1741 tcctccctgg gtattgacgc atgggtcatg gtagccgggt gaccaaccaa agattcaacg
     1801 ccgccaaggg attcggcaag tgagaacatt ctgaactttc taagtacggc ttttgcttct
     1861 tccgtactat cctttttaag gtcaaaggac accatggctc cgaagtcttt catctgagca
     1921 acagcaagtt catgactggg atgtgtcttg aatccgggaa aattgaccct gttgactgcc
     1981 ggatgtgtct tgagaaactc ggctattctg gcagcattgc ggcaggctcg tttcatcctc
     2041 acatggagtg tcttgatacc tctcaacacc aggaagcaat ccatcgggcc gggtgtggca
     2101 ccacatgcgt tctggaggaa gcgaagctgg tcttcaattt cctttttacg acacactaca
     2161 gcacccatga ccacatcgct gtggccgccc aggtatttgg ttgcactatg caaaacgata
     2221 tcagctccca gatcgagcgg ttgttgcaga tagggtgatg caaaggtatt gtccacacag
     2281 gtgattatcc catacttctc tgctaccttg gtcaccttgc gtatgtcgac tatattgagc
     2341 atcggattgg taggtgtctc aatccagatc agcctggttc gtgggttgat cagtcgctca
     2401 agctgcgcgg tatcattgag gtcgataaag tgactcttga tgccgaatct gccatatact
     2461 cgtgtaatca gtctgtaaga tcccccataa agatcgttgg tggagatgat ttcatcaccg
     2521 gggcttagca atttcaggat agtatccatg gcaccaagcc cactggaaaa gcaaagtcca
     2581 aatgtgccat tttcaagggc tgccagattg gcctcaagga cagttcgagt cgggttggct
     2641 gttctcgcat attcataacc cttatgtaca cccggttctt cctggacata ggtcgaagtc
     2701 tggaagatgg gcgtcataat agcacccgta ctcggatccg gctcaatgcc ggcatgtaat
     2761 aacttactgt tgaatttcat cgatcttggt gcggaagcac cagacttagc ttttttggcc
     2821 atgagattac tttcttttta aaactttatg ggcagcggca acgatatctt ctgcatcaag
     2881 accgtacttt ttaagtaagt caagcggttt gccactttca ccgaatttat catttacacc
     2941 aacaaattcc aatgggtaag gcttgttttg ggtgacgaga tgagctatgg catcgccgag
     3001 accaccattg atctggtgtt cttcacaaac cacgccgcaa tgcgtctttg aaaacgaatc
     3061 caggatagta tctgcatcca acggcttgat ggtaggcaca ctgatgacat caactgaaac
     3121 gccccgggct tccagagatt ttgcagcctc tactgcattc cataccatat gaccggtagc
     3181 gaaaaggctt acatctgtac ctgatcccaa tctgtctgcc ttgccgatga caaatggtgt
     3241 ctctgcatca gtgaaaacag gccaatccgg acgaccaaac cgtagatatg cagggccttg
     3301 atggtcaacc aaagcaagta ccgcctgcct ggtttgattg gcatcgatgg gacaaataac
     3361 tgtcattccc ggcaacatac gcatcaagcc tatatcttca agaatctggt gtgtggcacc
     3421 atcctcaccc agggtcaaac cggcgtgtga tgcacaaatt ttcacatttt ttccggaata
     3481 ggcaatggat tgtcttatct ggtcatatac acgtcctgtg gagaaattgg cgaaagtgcc
     3541 ggtaaatgga atcttgcccg ctgtcgccaa tcctgctgcc acgcccatca tattagcttc
     3601 cccgataccc atctggacga atcggtcagg aaatgcttca ataaattgtt ccagtttcag
     3661 ggaaccgagc aggtcggcta ccatagctat gacattctta tttctttttc cggcttcaaa
     3721 gataccttca ccgtatccat cacgggttgc ttttttaccg gttgctacta tatcgtggat
     3781 attcatgatt taatgttttg gtagtggagc ctgataattt tcgttatata catagtcacc
     3841 taaggtttct tccagctgtg caagcgcgat ctcaacctga tccttgtttg gggcctttcc
     3901 gtgccatttg ttggtaccca tcataaagtc aacaccttcg cccatattgg tgcgcatcag
     3961 gatgaccgtt ggaatttccg agccgcgatg agtcttagcc tcttccaagg cgttaactat
     4021 ggactccata tcattcccat tggtcaccac aacccgccag ccgaaggtac gccatttgcc
     4081 ttccagatcg ccaagactca ggatttcatt atttggacca tcgatctgct ggccattcca
     4141 gtcaacgatg acaataaggt tatccaattt atgaaatgga gcgaacatgg cagcttccca
     4201 aatctgacct tcctgcaact ctccgtcacc ggtagagaca aaaacaaaac ccggatcatt
     4261 gtccatcttc ttagcaagag cgataccgac accaactgaa aaaccctgac ccagcgaacc
     4321 ggtggcaact cttatacccg gcaaaccttc atgagtagtc gggtgaccct gaagccggct
     4381 gtttagtttt cggaaagtag atagttcctc cagcggaaaa tatccgcgcc gcgctaaaac
     4441 actgtatagc aacggtgaaa tatgcccatt ggacatcaca aaggcatcct ctcccctggc
     4501 atccatctta aactcctccg actggtccat gacgctgaag taaagtgccg tcaataattc
     4561 ggcgcacccc agtgagccac ccggatgacc gctggaagca atgtagacca tgcgcagtat
     4621 atcgcgccga acctgggtag ctattatctt aagctgccca a
//
LOCUS       DAOQQN010000263         4644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8592_length_4644_cov_6.058183, whole genome shotgun sequence.
ACCESSION   DAOQQN010000263 DAOQQN010000000
VERSION     DAOQQN010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8592_length_4644_cov_6.058183"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PLG24_09745"
     CDS             <1..389
                     /locus_tag="PLG24_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25216.1"
                     /translation="HRGKYEALVQQKPVTVIRDYDRDAILDFNNGEKQTGHFGINIHR
                     ASISGTTKSIDKYSAGCQVFKSADDFLVFMELCKNHAKLYGNKFSYTLIDFRSLRRIT
                     LKRVVTATTIFAAIALGWVFKPNVKL"
     gene            386..2851
                     /locus_tag="PLG24_09750"
     CDS             386..2851
                     /locus_tag="PLG24_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25217.1"
                     /translation="MSIPKPPFHTASEELLYNIYLKIQNVDYLRESDISTLAKLNAIL
                     IDADLMNSEDILSAINAVKGNVPEAGNTLEKLFNIIQGLTYLKADDIDTLAELNAILS
                     DADLIKTEDLQNAITALKGNAPLVADTLEKIYNLLQPILSAWVQDGNNVGATKSIGTK
                     DNFPLPFITNGIERGRFDLSGNLLIGHSGAVGGRLHIRAASTLEAGFSVFIDDPTLNL
                     IFGVTNTGKLFVNKDRPNLLVNGGWQGMTGLKNVILTSGSVGARLVDTGGDNTIIGYN
                     ASYSTGGNPARGLTRNVVVGSEAGSSDNNGTSFSDCVYIGYRAGNMIGFNTGHRNTML
                     GASAGATAGQSGTDHTLVGYDAQNARSNSFNTLLGSGTRAYNSLGDIVSNGGGQSYMT
                     AIGAGAAVRTPNTLVLGRVLNDQIVVGAESCNFQLPGTTANLGFNGYKFQVVRPGGAA
                     LGISGSSFFRDGNFSITLGEQGNGAVKNSFRINSYLSGYGGYEFLLSSNNVNATLEIA
                     DYQFGGTKTEMLRLSPGGYANTAILVRPNDIYHTGYGFKYLIQGVNTGLQSRFISAFH
                     GQMNLSVDDSFPNYNRAQVYTALASVTNHFVDGFFADVRATNANAQAFAFRSTGSRIF
                     FDGTGMTFTTPVTSINTDGSAHAGATALLLNGSYIISAALYIVPLGSERTGMIISPGQ
                     TQANGLFISPQQSLGTYNYDGYLMWLQYSTTGNNMMGGTTKPMLLIRKHNAVLNGFDH
                     TGAFLRMEENIGSTGAFVEAFKYDAVSNSLKLKFSVNKDGVVSLGQVTIDPASIAGVG
                     RLYFVGEDLKFVTPSGVVRTVKF"
     gene            2864..3307
                     /locus_tag="PLG24_09755"
     CDS             2864..3307
                     /locus_tag="PLG24_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25218.1"
                     /translation="MSRNSIVVNLPELDSTTNKRKKVIHDYLIIKSNLEMRHGIILEF
                     TEGDGNPIVPSNELQRQLFLPKQITKETDGKFTNPLTGIEVSSETPNAITELEYWQNI
                     GLNSFPGISSMPNEVREALKDIKISDLVYWLIEQSILKGNAEGKY"
     gene            3321..3707
                     /locus_tag="PLG24_09760"
     CDS             3321..3707
                     /locus_tag="PLG24_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25219.1"
                     /translation="MTNKEAYKLITALMDTPAPAGTKLEHARNQTLKNASSFVEAYND
                     KLEDLNIDYCSTDDKGNIIRDSRGQYIFTKDNQRALSKELKKFMDSELIVPFEIVSTT
                     DKKGLSDPQVEYLTEVGFIRGLMTVI"
     gene            3821..4525
                     /locus_tag="PLG24_09765"
     CDS             3821..4525
                     /locus_tag="PLG24_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25220.1"
                     /translation="MTPEEEIDGNIQSLVDDLTKVTVRIATWDAGILKGRSSGFLYRS
                     PNHDSPLVITAGHNMPDEGSFIETRVKNDNDEMLQINAGKFQVFYNREKIDVAFSELP
                     IELYKKEITQYEGVELFPYFREFVKAISGEAYSFAVINNYEFVKSGNGFILPVYLCYE
                     VGLELVDQDESFNYFKTNRNFQGHEYYQGASGSPIIDPEGAITSILVSGFDDIGILRG
                     FRLDNFDFKSDVAKKA"
BASE COUNT         1467 a          850 c         1022 g         1305 t
ORIGIN      
        1 aacatcgagg aaagtatgaa gctcttgttc agcaaaagcc agtaaccgtt atacgagact
       61 atgaccgtga cgctattctt gatttcaaca atggcgaaaa acaaacagga cattttggaa
      121 tcaacattca tagagcttcc atttctggca caaccaaatc tattgataag tattctgccg
      181 gatgccaggt gtttaaaagc gcagatgact ttctggtgtt catggagctt tgtaaaaacc
      241 atgcaaagct ttatggaaat aaattcagct acacactgat tgatttcaga tcgctcagaa
      301 gaattacgct caagcgagta gtaacagcga caaccatttt tgcagccatc gctttaggat
      361 gggtgtttaa accgaacgtg aaattatgag tattccgaaa cctccatttc atacagcttc
      421 agaggaactg ctttataata tctacttaaa gattcagaat gtggattatc taagggagag
      481 tgatattagt acgcttgcca agttgaatgc aatcctaatc gatgcagact tgatgaatag
      541 cgaagatatt ttatcggcta tcaatgcggt gaaaggtaat gttccggagg ctggcaatac
      601 gcttgaaaag ttatttaaca tcattcaagg attgacatat ctgaaggcag atgatattga
      661 tacgcttgca gagttgaatg caattctctc cgatgctgac ttgataaaaa cggaagacct
      721 tcaaaatgcg attaccgcat taaaaggaaa tgcccccctt gttgctgata ccttagaaaa
      781 gatttacaat ctacttcaac caattctctc agcatgggta caggatggaa ataatgtggg
      841 agccactaag tctattggta caaaggataa ttttccactt ccctttataa ccaatggtat
      901 tgaacgagga agatttgatt tgtcgggcaa tttattaatc ggtcattccg gagcggtggg
      961 tggtcgttta cacattagag ctgcgtcaac attagaagca ggtttttctg ttttcattga
     1021 tgatcctact ttgaatttaa tctttggagt gacgaatacc ggaaagctat ttgtcaataa
     1081 ggatcgacct aacctacttg ttaatggtgg ttggcaaggg atgacaggac ttaaaaacgt
     1141 catccttacc tctggctcag tgggtgcaag actggttgat actggaggcg acaacaccat
     1201 catcggttac aacgcttcat atagtactgg aggaaatcca gcgagagggc ttacacgaaa
     1261 tgtagtggtt ggttcagagg caggttcttc tgataacaat ggaacgagct ttagtgactg
     1321 cgtgtatatc ggctatcggg cgggtaatat gatcggtttc aatactggtc atcgaaatac
     1381 aatgctgggt gcttctgccg gagcaacggc aggacaaagt ggaactgatc atacgctggt
     1441 tggctacgat gcacaaaatg caaggtcaaa ctctttcaat acgctacttg gttctggtac
     1501 gagagcatac aacagtcttg gagatattgt aagcaatggc ggtggtcaga gttacatgac
     1561 tgcgataggc gcaggtgcag cggtgagaac accaaataca ttagtacttg gtcgcgttct
     1621 taatgatcag attgttgttg gtgcagagag ttgcaatttt caattaccag gaacaacagc
     1681 taatctggga ttcaatggtt ataaatttca agtcgtcaga cctggaggtg cagcacttgg
     1741 aatttccggc tcttcattct ttcgagatgg taatttctca attactcttg gtgaacaggg
     1801 aaatggagct gtaaaaaatt catttcggat taacagctac ttgagcggtt atggagggta
     1861 cgaatttttg ttgagttcaa acaatgtaaa tgctactctt gaaattgcag actaccaatt
     1921 tggcggtact aagactgaaa tgctccgcct gtcgccagga ggatatgcta atactgccat
     1981 acttgtaaga cctaacgaca tctaccatac aggctatggt tttaagtatt taattcaagg
     2041 tgttaacaca ggattacaaa gtcgattcat atctgcattt cacggacaga tgaatctatc
     2101 cgttgatgat agcttcccaa attataatcg tgctcaagtc tacactgcac ttgcaagtgt
     2161 aaccaatcat tttgtagatg gattctttgc agacgtaaga gctactaatg caaatgccca
     2221 agcatttgcc tttcgctcta ctggtagtcg aattttcttt gatggtactg gaatgacttt
     2281 taccacaccg gtaacaagca taaatacgga tggttctgct catgccggag caactgcatt
     2341 attgcttaat gggagctaca tcattagcgc agctctgtat atcgttccgc ttggttctga
     2401 aaggacagga atgataatat ctcctggaca aacacaagcc aatggtttgt ttataagtcc
     2461 acaacaatca cttggcacct ataactatga tggttacctc atgtggcttc aatacagcac
     2521 aaccggaaat aacatgatgg gaggtactac caagccaatg ctgctcattc gaaaacacaa
     2581 tgcagtatta aatggatttg accacacagg agcattccta agaatggaag aaaacattgg
     2641 ttctaccgga gcatttgtag aagctttcaa atatgacgca gtgagcaatt ccttgaagtt
     2701 aaaattctca gttaataaag atggagtcgt ttcacttggc caagtaacaa ttgatcctgc
     2761 aagcatcgct ggagtaggaa ggctatactt cgttggtgag gatctcaagt ttgttacacc
     2821 atcaggagta gttagaactg taaaatttta acatagaata tttatgagta gaaattcaat
     2881 cgttgtgaat cttccagaat tagattcaac aacaaacaaa cggaaaaagg ttattcatga
     2941 ctatctcatc attaagagca atttggagat gaggcatgga ataatcctgg aattcacaga
     3001 aggagacggt aatccaatag tgccaagtaa tgaacttcaa cggcaactat tccttcccaa
     3061 acaaattacc aaggaaacgg atgggaaatt cactaatcca ttaacaggaa ttgaagtgag
     3121 ttcagaaaca ccaaatgcca ttacagaact tgagtactgg caaaacattg ggctgaattc
     3181 attcccaggt attagctcaa tgccaaatga agtcagagaa gctttgaagg atattaaaat
     3241 ctctgacttg gtctattggc tgattgagca atcaatctta aaaggaaatg cagaaggaaa
     3301 atattaaatc agtatcactc atgacaaaca aagaagctta caaactaatt acggcattaa
     3361 tggatactcc ggcacctgct ggaacgaagt tagagcacgc cagaaatcag acattgaaga
     3421 atgcctcgtc ttttgtcgaa gcctacaatg acaagctgga agacctcaac attgattatt
     3481 gcagtacgga tgacaaaggc aacatcatcc gtgattcgag aggccagtat atttttacca
     3541 aggacaacca aagggcactc tctaaggaac ttaaaaagtt catggatagc gagctgattg
     3601 ttccttttga aattgtatcc acgactgata agaaaggtct aagcgatccc caggtcgaat
     3661 atctgaccga ggtaggattt attcgaggat taatgacggt gatttaggaa aggctattat
     3721 ttttgcccaa aatattgatg taggagcccg aaaaggagtc acacgaaagt gtggctcctt
     3781 ttgttttaag ggagtatatt aagtgcttat tacgattgat atgacaccag aagaagaaat
     3841 agatggaaac attcaatcct tggtggatga tttgacaaag gtaactgtga ggattgccac
     3901 atgggatgct ggaattctaa aaggacgcag ttcaggattt ctatatagat cccccaatca
     3961 cgatagccca cttgtcatta cagccggtca taatatgcca gatgaaggat cattcattga
     4021 gacaagagta aaaaatgaca atgatgaaat gcttcaaata aatgcgggca agtttcaagt
     4081 cttctacaat agggaaaaaa ttgatgttgc ttttagtgag cttccaattg agctctataa
     4141 aaaggaaata acgcaatacg agggtgtcga actttttcca tacttcaggg aattcgtaaa
     4201 agctatttct ggagaggcct atagttttgc ggtgattaat aattatgaat ttgtcaaaag
     4261 tggcaacggt tttattcttc cagtatatct atgctacgaa gtcggattgg agctagttga
     4321 tcaggatgaa agcttcaact actttaaaac gaatcgaaat tttcaaggtc atgagtacta
     4381 tcaaggagca agcggctccc ctataataga tccggagggt gcaataacct caatattggt
     4441 aagtggtttt gatgatattg ggatattgag aggttttagg ctcgacaact ttgacttcaa
     4501 atctgatgtg gctaaaaagg cctaaagttc taaatccgaa aactttcgta gattttctat
     4561 gaattggttc gatattcgta accttgggac tactatgatg gcaactcgaa aaaacgggtt
     4621 gcctttttta ggtctgctgc ttac
//
LOCUS       DAOQQN010000264         4643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8594_length_4643_cov_5.251744, whole genome shotgun sequence.
ACCESSION   DAOQQN010000264 DAOQQN010000000
VERSION     DAOQQN010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8594_length_4643_cov_5.251744"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /gene="fdhD"
                     /locus_tag="PLG24_09770"
     CDS             <1..786
                     /gene="fdhD"
                     /locus_tag="PLG24_09770"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HMZ25221.1"
                     /translation="LIIPVNIERLSEDMVFSKQDVVMIEQPLQMDALGPEGNMSRISI
                     TMRTPGHDAELALGFLFNEGLLQTGDAASVANHTDNNILIKMNHNIQGQASKRNFLMT
                     SSCGVCGKSELNDLKINAPVRKIVHTLDHRNISSMYEWLKKEQKAFAETGGCHGAGVF
                     DYEGHMITGREDVGRHNAVDKCAGYLWENNLLEKEEYVLCLSGRGCYELIQKAIRMNC
                     TTVICIGAVTSLAIETAYQYGITLIGFFKPYNFNIYTYKERII"
     gene            847..2064
                     /locus_tag="PLG24_09775"
     CDS             847..2064
                     /locus_tag="PLG24_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HMZ25222.1"
                     /translation="MNIRILLTLSLLTLCKFSLSADHFRIIGYLPTYRFATLEKLEVE
                     KLTHLNLAFAQATYDGKLVLGGNFNYIVSRSKERNPDLKVLVSLAGGGIKGATFANWR
                     RNMAPGRKTEFIRQIMDFVRKNNFDGVDVDLEWGNVTPEYPEFVAELSDSLRSNNLEI
                     SAAFPAMKLYSVLNPESLKMFDYINIMAYDNSGPWQPRVVRQHSSYELAQNSIDFWAE
                     VCQLPKEKIVLGIPCYGWEFGKSVKSKTFSSLVSEKKEFAFTDNIRKIFYNSIPTIIE
                     KTKLARERCGGVMLWEIGQDAIGDKGDYSLLHFVHTTALTEGRNFLPKIRKFTIEQSN
                     ESIVSIRLEDQVAPGSYLVLEDIIGTANQKIDLCHGDSEVNVDLTIYPRGVYVISLIE
                     PGALPRSQRITKL"
     gene            2069..2770
                     /locus_tag="PLG24_09780"
     CDS             2069..2770
                     /locus_tag="PLG24_09780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ25223.1"
                     /translation="MVIVVTGSTRGIGKAITERFAADASIIFITARNEKALTEQESRL
                     KSDYPDLVVRKFAADLSKHDNVVAFARFVLDNCTAVDILVNNAGIFTPGNLLNEDEGN
                     FDLVMETNVRSIYTLCRMLVPKMIEARKGHLFNICSVAGLKAYPNGGSYSVSKFALLG
                     LTKNLRFELAPHGVKATAVIPGATWTDSWSNSGLPRSRFMEADDIAAMIYHISRLSPA
                     CVVEEIVLRPQLGDI"
     gene            complement(2772..4040)
                     /locus_tag="PLG24_09785"
     CDS             complement(2772..4040)
                     /locus_tag="PLG24_09785"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HMZ25224.1"
                     /translation="MSQVNYSLIRIEHLSLRLKSSQLSELINNALRMALQKKDSFAVN
                     ILNDQEILITPQPSVTINIEDNKIKGILPLRISTNLKGPIKRVSVKAGILAHFEAEIE
                     RLDENNLGIHSDISAIEWTDEIDIQPPLLDFVLRDSFVKEMVENQLPAINIKINEALR
                     DFLDFGNLLSQAELNRKIRIPLKNKQEMFLFVSPESIEVNKMGLDGDNLQFSCNISGR
                     ILPSENMDDTFPTVTPRVIFLDQPASGNELLIDIRLDLASLGEPVLDVVKSMPNIENA
                     IGCNIEKVVVRPVGTDGVAIGLKLKGALNGGLSIAGRPVVDQNSLMLDIENLTFDFSG
                     KNILSSLKGNIALSVAKSVIRKQFPIGLRPYIDSMIRATNDMISSFKLIDGLILQGNI
                     GEWHLQEMDIVDGRLVIIFRTEVVTFLSPA"
     gene            4412..>4643
                     /locus_tag="PLG24_09790"
     CDS             4412..>4643
                     /locus_tag="PLG24_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25225.1"
                     /translation="MLLFIRRFLLTSLGIFLSFSIFAQFTVNTSVTAMAMAQELVGPG
                     VEVISATYSGDPSSKGIFVATGTSLPMTNGVVL"
BASE COUNT         1405 a          913 c          940 g         1385 t
ORIGIN      
        1 ttaattatcc ctgtaaatat tgaacgcctt agcgaagaca tggtgttttc caaacaagac
       61 gttgtcatga tcgaacagcc tttgcagatg gatgcattag gtccagaagg taacatgtcc
      121 agaataagca tcaccatgcg cactccgggt catgatgccg aattggcttt aggcttcctt
      181 ttcaatgaag gcctattgca aacaggtgat gcagcatcag tcgcaaatca caccgataat
      241 aatattttga taaaaatgaa ccacaatata caaggacagg catccaaaag aaatttttta
      301 atgacttcca gttgcggtgt atgtggtaaa tcagaactca atgatttaaa aataaatgca
      361 ccggtaagaa aaatcgttca cactttggat cacaggaaca tctcatccat gtatgaatgg
      421 ttgaaaaaag aacaaaaagc ttttgctgaa acaggtggct gccacggtgc cggagtattt
      481 gactacgaag ggcatatgat aacaggaaga gaagatgttg gccggcataa tgctgtggat
      541 aaatgtgccg gctatctatg ggaaaataat ttactggaaa aggaagaata tgtcctttgc
      601 ctaagtggca ggggttgtta tgaactcatc caaaaggcaa tccgtatgaa ttgcacaaca
      661 gtaatctgta ttggtgcggt gaccagtttg gctattgaaa cagcttatca gtacggcatc
      721 accctgatcg gattctttaa gccatataat ttcaatattt acacatataa agaaagaata
      781 atttaaaaat ttttatatat atgaaattta attatatttg cgcattgtat gttaatacat
      841 tgcaggatga atataaggat tttattgact ctttcgcttc tcaccctttg caaattctca
      901 ctttccgctg atcatttcag gattattggc tacctcccta cctatcggtt tgcaacgctt
      961 gaaaaactgg aggttgaaaa attgacacat cttaatctcg cttttgccca ggcgacttat
     1021 gatggcaaac tggttttggg tggcaatttc aattatattg tatcgaggtc caaagaacgg
     1081 aatcctgact taaaggtttt ggtgtcattg gccggaggag gaatcaaggg tgccaccttt
     1141 gccaattggc gcaggaatat ggcacccgga agaaagactg aattcatccg tcagattatg
     1201 gacttcgtca gaaagaataa cttcgacgga gtggacgtcg atctggaatg gggcaatgtt
     1261 actcctgaat accctgaatt tgtagcggaa ttaagtgatt ctctacgcag caacaacctg
     1321 gagataagtg ctgccttccc tgctatgaaa ctatacagcg tgctgaatcc cgaaagtctg
     1381 aaaatgtttg attatatcaa catcatggcc tatgacaaca gtggtccgtg gcagccaagg
     1441 gtagtccgcc aacattcttc ctatgaactt gctcaaaatt ccatcgattt ctgggcagaa
     1501 gtttgtcagc ttccaaaaga aaaaatcgtt ctcggcatac catgttatgg gtgggagttt
     1561 ggcaagtcag tcaaaagcaa gactttttca tccctggtta gtgaaaaaaa ggagtttgct
     1621 tttacggaca acatcagaaa gatattttac aatagcatcc ctacaataat agaaaagacc
     1681 aagctggcaa gagaaagatg cggtggtgtg atgttatggg agattggtca ggatgctatt
     1741 ggcgataaag gtgattattc tctattacat tttgttcata ctacagcatt aacagaaggg
     1801 agaaattttc tccccaaaat aagaaaattt acaatcgaac aaagtaatga aagcatcgta
     1861 tccatcaggt tggaggacca ggtagcacca ggatcttatt tggtcctgga ggatatcatt
     1921 ggtaccgcca atcaaaagat agatctttgt catggagata gtgaggtcaa tgtggatctc
     1981 actatctacc ctcgcggagt ctatgtcatc tctctgattg aaccgggagc attgcccaga
     2041 agtcaacgaa ttacaaaatt atagcctcat ggttattgta gttacaggta gcacaagagg
     2101 cataggtaag gcaataacag aaaggtttgc agcggatgca tccataatct tcataacggc
     2161 gcgaaacgaa aaagcgttaa cagagcaaga aagtcgatta aagagtgatt atcctgacct
     2221 ggttgttaga aaatttgccg ctgacctctc taaacatgac aatgtggttg cctttgcacg
     2281 atttgtattg gacaattgca ccgcagtgga tattttggtc aacaatgccg gaatatttac
     2341 tccgggtaat ttattaaatg aagatgaagg taattttgat ctggtgatgg agaccaatgt
     2401 aagaagtatt tatacactat gtcgaatgct ggttcctaag atgatcgagg cccgaaaagg
     2461 ccaccttttc aatatctgtt ctgttgcggg gctaaaggcc tatccaaatg gaggttctta
     2521 ttctgtgtcg aaattcgccc ttttgggttt aacgaagaat ttacgttttg aactggcccc
     2581 tcatggtgtc aaggcaacgg cagtcatccc gggtgcaacc tggactgaca gctggtccaa
     2641 ttcgggtctg cccagatcaa ggtttatgga agctgatgac atcgctgcaa tgatttatca
     2701 catatcgaga ttaagcccgg cgtgtgtagt ggaagaaatc gtattgcggc ctcaactggg
     2761 ggacatttga tctatgccgg acttaaaaag gtcacaactt ccgtacggaa aataataacc
     2821 agacgaccat ctacaatatc catctcctga agatgccatt cgccgatatt gccttgcagt
     2881 ataaggccgt caatcaattt gaatgaagaa atcatatcat tggtggcacg gatcatacta
     2941 tcgatatacg gcctcaatcc aattggaaat tgttttctga tgacggattt ggctactgaa
     3001 agcgctatgt ttcccttgag tgaggaaaga atatttttac cactgaagtc aaaagtcaga
     3061 ttttcaatat cgagcatcag cgaattttga tcgactaccg ggcgacctgc tatggacaga
     3121 ccaccattga gtgctccttt cagtttcaat ccaatggcaa caccatcggt ccctaccggc
     3181 cggactacta ccttttcaat attgcatcct attgcatttt caatatttgg catggacttc
     3241 acaacatcca gaaccggctc acccagcgac gccaaatcaa gtcgtatatc gatcaacaat
     3301 tcatttccgg atgcaggttg gtcaagaaaa ataacccggg gtgtgacagt gggaaatgtg
     3361 tcatccatat tttcagaggg aaggattcga cctgaaatat tgcaactaaa ctgtaagtta
     3421 tcaccatcca gccccatttt attaacctca attgattcag gcgatacaaa aaggaacatc
     3481 tcctgtttat tctttaatgg tattctgatc ttcctattca attcggcctg ggacagtaaa
     3541 ttaccaaaat cgagaaagtc tctcaacgct tcattgatct taatattgat ggcaggtaac
     3601 tgattttcca ccatttcctt cacaaaacta tcacgcagga caaaatccag caaaggtggc
     3661 tgtatgtcta tttcatcagt ccactcaatc gctgaaatat cagaatggat gcccagatta
     3721 ttttcatcaa gtctctcaat ctcagcctca aaatgcgcaa ggataccggc ctttacactt
     3781 acccgtttga ttggtccttt caggttggta cttattcgta acggcaagat ccccttgatc
     3841 ttgttatctt cgatattgat cgtaacactt ggctgtgggg taatcaggat ttcctgatcg
     3901 ttgaggatat tgactgcaaa tgaatctttt ttttgcaatg ccattctcag agcattgttt
     3961 ataagttcag acaactggct gctcttcaat cgcaaactca aatgttcgat tctaatcaat
     4021 gaatagttaa cttgtgacat acaggtgcta agatatgatt tatacaaaat tagttgaagt
     4081 attttccaaa aatttccggg acaattattt ttcccaagaa tctcgttgtt cgactaaaaa
     4141 tatttctctt ttgataattc tataaataca cgacaactca aaatatacat caattacgca
     4201 aatacataat ttaatttttc agtatacata ttttaataaa tataattctt ttaatcaaaa
     4261 taatacctac caattctttt atgttcttgc actcaattct tatagaaatt cttcaataaa
     4321 gattatttta catacaattt tattacattt acatttgtaa aaacgaatta taatttcagt
     4381 catctctttt tggacttttt ttaagacgat catgctttta tttattcgga gatttttact
     4441 tacttcactt gggatttttt taagtttttc cattttcgct caatttacgg tcaacacaag
     4501 tgtcacggca atggctatgg cgcaggaatt agtaggtccc ggtgttgaag tgatttccgc
     4561 tacttattcc ggcgatccaa gttccaaagg tatatttgtc gcaacgggca cttcattgcc
     4621 aatgacaaat ggtgtagtcc ttt
//
LOCUS       DAOQQN010000265         4627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8631_length_4627_cov_5.774934, whole genome shotgun sequence.
ACCESSION   DAOQQN010000265 DAOQQN010000000
VERSION     DAOQQN010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8631_length_4627_cov_5.774934"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /gene="uvrC"
                     /locus_tag="PLG24_09795"
     CDS             <1..1495
                     /gene="uvrC"
                     /locus_tag="PLG24_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HMZ25226.1"
                     /translation="VFFTRKVIKDGSTYFGPFTSKFRVQQILDLIKNLFPLRTCNLNL
                     TPENIRKGKFKVCLEYHLKNCMGPCEGLESEEAYNAKIDQIKNIMRGNFAPVKAYLRE
                     QMDAFAENLEFERAAEMKNKLSLFEDYQAKSMVVSTTIRDVDVFAYHAEENEFYINYI
                     KVVNGMMINTFLQEGIRNLDDDAFNFSYGIRQLRERFNSIAPEVLVNMEGVILDEKEL
                     KVQIPSIGDKRKLMEMAEKNIHFYLLQKRKESINAPRVTPTQRIMETLQKDLNMDRLP
                     NHIECFDNSNIQGTNPVSSCVVFKNAKPAKSEYRKFNVKTVDGPNDFATMEEVVTRRY
                     TRLLEEGQPLPDLIIIDGGKGQLGVAAEVLTKLGILGKVTLIGIAKRLEEIYFPGDSL
                     PLYINKKSESLKLIQHLRNEAHRFAITFHRKKRSMNFTVSELTQIDGIGKATAEKLLQ
                     HFKSVKRIAAASPEELAGVTGKRAANIVFKYYNRAEEEGEEEVPEVG"
     gene            1747..1989
                     /locus_tag="PLG24_09800"
     CDS             1747..1989
                     /locus_tag="PLG24_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HMZ25227.1"
                     /translation="MSQSIEEKVRKIIVERLGVDESEVTLEASFTNDLGADSLDTVEL
                     IMELEKEFNISIPDEQAEQIQTVGDAVSYLESQINA"
     gene            complement(2069..2569)
                     /locus_tag="PLG24_09805"
     CDS             complement(2069..2569)
                     /locus_tag="PLG24_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25228.1"
                     /translation="MTLGEFIDWIGRDPKYIICFFLLMPVGAALMWFIAKDKGHISPW
                     KYIYSILIYSVCIPGVSAVAFNLYLFVFQRQDVFAMDIFTQVMPILSMVLTLWLVRRN
                     VAFESIPGFDKLSQLITIIFAVLMLLWLGDRVRIYSITFVPLWMFILIFLGLILLIRY
                     AWSKLF"
     gene            complement(2610..3374)
                     /locus_tag="PLG24_09810"
     CDS             complement(2610..3374)
                     /locus_tag="PLG24_09810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HMZ25229.1"
                     /translation="MKIYSWNVNGIRASMGKSFLEKFNAFDADIFCLQETKAQEDQVE
                     KALEEVQGYHLYANSAEKKGYSGVAILTKQKPLSFQKGIGVDVHDMEGRVLTLEFDNF
                     YMVNVYVPNSGDQLVRLEYRAEWDDAFSSFLAALRRQKPVVVTGDFNVAHTQIDLARP
                     KENYNKTSGYTQVEIDGMDLILNAGFSDAFRKLYPEKIQYTFWSMRFGARAKNMGWRI
                     DYFLVDKDIDSKITECTIHDQVMGSDHCPISLNIDL"
     gene            3945..>4627
                     /gene="pepT"
                     /locus_tag="PLG24_09815"
     CDS             3945..>4627
                     /gene="pepT"
                     /locus_tag="PLG24_09815"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripeptide aminopeptidase PepT"
                     /protein_id="HMZ25230.1"
                     /translation="MSLPKFNYTVTDRFLHYVTIDTQSDPGSPTTPSTQKQKDLGRVL
                     VSQLLEMGLTDAHMDEHGYVYATLPSNVDHEVPVICFCSHMDTSPDCSGTDVKPIIHR
                     NFDGGKIVLPLDNSQVITIDGHPALADQIGNDIITASGDTLLGADNKSGVAEIMDAVF
                     QMIQHPEIKHGKLRILFTPDEEIGRGVDKVDMVKLAADFGYTMDGETVGHVEDENFNA
                     KAAVVKITGV"
BASE COUNT         1437 a          914 c         1030 g         1246 t
ORIGIN      
        1 ggttttcttt acccgaaagg tgattaagga cggttcgacc tatttcgggc catttacttc
       61 caaatttagg gtacagcaga ttctggacct gatcaagaac ctgtttccac tgcgtacatg
      121 caatctcaac ctgactcccg aaaatatcag gaaaggaaaa ttcaaggttt gtctggaata
      181 tcatttgaaa aactgtatgg gaccttgtga aggattggaa agtgaagagg catacaatgc
      241 caagattgac cagattaaaa acatcatgcg cggcaatttt gcaccggtaa aggcgtatct
      301 gagggaacag atggatgcat ttgctgaaaa cctggaattt gagcgtgctg ctgagatgaa
      361 aaacaaacta tcgctgtttg aagactatca ggctaagtcc atggtagtca gtacaaccat
      421 cagggacgta gatgtctttg cctatcatgc cgaggaaaat gagttttata tcaattatat
      481 caaagtggtc aacggcatga tgatcaatac atttctccag gaaggtattc gtaatctgga
      541 tgatgacgca ttcaattttt cctatggtat ccggcagcta agggaaagat ttaacagtat
      601 cgctccggag gtcttggtca atatggaagg tgtgattctc gatgaaaaag agctgaaagt
      661 gcagatacca agcataggcg ataagcgcaa actgatggag atggccgaga agaacatcca
      721 tttctacttg cttcagaaac gaaaggaatc catcaatgcg ccccgtgtta ctcccactca
      781 gcgaattatg gagacccttc agaaggatct caatatggac aggctgccca atcacattga
      841 atgcttcgat aacagtaata ttcagggtac caatcctgtc tccagctgcg tggtatttaa
      901 aaatgccaaa cctgccaaga gtgaataccg caagttcaat gttaagacag tcgatggccc
      961 caatgacttc gcgacgatgg aggaagtagt gacccgaaga tatacaaggc ttcttgaaga
     1021 agggcaacca ttgcctgatc tcatcatcat cgatggtggc aaaggccagt tgggggtggc
     1081 tgcggaggta cttaccaaat taggcatctt aggtaaggtt acgctgatcg gaatagcgaa
     1141 gaggctggaa gaaatatatt ttccgggtga ttccctgcca ttgtacatca ataagaaatc
     1201 cgaatcgctc aagctcatcc agcatctgag gaatgaggcc catcggtttg ccataacttt
     1261 ccaccgcaag aaaaggtcga tgaattttac agtatctgaa ttgacccaaa tcgatggcat
     1321 cggaaaggcc acagctgaaa aactgttgca acacttcaaa agcgtcaagc gaattgcagc
     1381 tgcctctccg gaagaactgg ctggtgtcac cgggaaaagg gcagccaata tagttttcaa
     1441 atactataac cgggctgaag aagaaggaga agaagaagtg ccggaagtcg ggtaatgatg
     1501 tgttataatc taaaaattca attgagtgat gagtgttgaa gaaaagattt gagaacagcc
     1561 ggcgaaaaat ctaaaaaaat attgttggaa aaagtgaccc aaagaattgc cgacgtccta
     1621 tatatgtatg aaaaaaagga aaatcattct ctcctgaaaa aataatgcct caaatctttg
     1681 tttaaaggtg taggaatgat ttactttgca tctttattta ataaatctaa cttaaacaaa
     1741 cgtaaaatgt cacaaagtat tgaagaaaaa gtcagaaaaa tcattgttga aagacttggt
     1801 gttgatgagt ctgaagtaac tctggaagca agctttacca acgatcttgg cgcagattct
     1861 ttagatacag ttgagttaat tatggagctg gaaaaggaat ttaatatctc cattcctgac
     1921 gagcaggctg agcaaattca aactgtaggg gatgctgtgt cttacctcga gtcacagatc
     1981 aacgcttaaa tcttaatata gagaaaaaag aaaggctgtc cgatcgggca gccttttgtt
     2041 tttataatga tttaatcaac ctaaaacttc aaaatagctt tgaccacgca tacctgatca
     2101 aaagaatcaa acccagaaaa attaatataa acatccagag cggtacgaaa gtgatcgaat
     2161 atatcctcac cctgtctccc agccataata acatcagcac agcaaaaatg atggtgatca
     2221 gctgactaag cttatcaaag cctggaattg actcaaaagc cacattcctc cgtaccagcc
     2281 atagtgtcag gaccatagac aaaatcggca tgacctgggt gaatatatcc atggcaaata
     2341 catcctggcg ttgaaacaca aaaaggtaga gattgaaggc gacggccgac acaccgggga
     2401 tgcatacgga gtaaatcaga atgctgtaga tatatttcca gggactgatg tgtcctttgt
     2461 ccttggcaat aaaccacatc aaagctgctc caaccggcat cagtaagaaa aagcatatga
     2521 tatacttcgg atcgcgacct atccaatcaa taaattctcc aagagtcata actagcttat
     2581 taatagggtt atataaatta gttttattgt tataaatcaa tattcaatga aatagggcag
     2641 tggtcactcc ccattacctg gtcgtgaatg gtacactcag taatctttga atctatgtct
     2701 ttgtccacca aaaaataatc tatacgccag cccatattct tggctctggc tccaaaccgc
     2761 atactccaga aggtatattg tatcttttcc ggataaagct tgcggaaggc atcagaaaac
     2821 cctgcgttga ggatcaggtc catgccgtca atctctacct gggtataacc actggttttg
     2881 ttatagtttt ctttcgggcg tgccagatcg atttgggtgt gtgcgacgtt gaaatcaccg
     2941 gtcaccacaa ccggcttttg cctcctaaga gcggcgagaa aggatgagaa ggcatcgtcc
     3001 cattcagctc tgtattcaag ccggacaagc tgatccccac tgttggggac atagacattg
     3061 accatgtaaa agttgtcgaa ttccaaggtc agcacccggc cttccatatc atggacatcc
     3121 actccaatgc ctttttggaa ggaaaggggt ttttgttttg tcaggatggc aactccgcta
     3181 tagcctttct tttcggcaga attggcatac agatgatatc cctgtacttc ttccaaagcc
     3241 ttttctacct gatcttcctg agctttggtc tcctgaagac aaaagatatc ggcatcaaag
     3301 gcattgaatt tttccaaaaa cgacttgccc attgaggcgc ggattccgtt gacattccag
     3361 ctgtatattt tcatgttcga ttgtattgtt atgtaaatat ggtgaaagtt gatgaaaatt
     3421 gggtagtcaa aaatagaagt atccggatca atattccata ggcatcaaag tggtgatggg
     3481 gccgggcaac ttgtccaaac caatacataa gcaactattg aaacccttct ttaccccggt
     3541 cataaggtag agtatatatg taaaaaattt tagctgtaac aggacactta aaaataaaag
     3601 atggtttgat gaatccgatt atacaatgga tgatttcacc ggcacaacag gaattataag
     3661 accaatattc aaacgattaa ctaaggataa aaggaatatc aggtcagaga aggagagatc
     3721 tcaaatcagg atttctgata atattttgtg tggtaggtaa ttttattttg tcaggtatgc
     3781 cttttttatc gggataatgg gcgtttttgg tcagtttgtt catttaatcg attggtttgt
     3841 gtgcaacttg tgtctatggt tatacttttt ttaagaatac atttttccct cattattctc
     3901 aacattatct taatttcacc gatcaaatca aaattctgat aaatatgtca ttgccgaagt
     3961 ttaattatac tgtcacagac cgatttcttc attatgtaac tattgatacg cagtcggatc
     4021 cgggcagccc gacgactcca agcacccaga agcaaaagga tctcggtagg gttttggtga
     4081 gtcagttgct tgaaatggga ttgactgacg ctcacatgga tgaacacggc tatgtatatg
     4141 caactcttcc atccaatgtg gatcatgaag tgcctgtcat ctgtttttgt tcgcatatgg
     4201 ataccagtcc ggattgttca gggactgacg tgaagcccat catccataga aatttcgatg
     4261 gcggtaagat tgtcctgcca ttggacaata gtcaggtaat caccatcgat ggacatccgg
     4321 ctcttgcaga ccagataggt aatgacatca ttactgccag tggtgacacc ctgttgggtg
     4381 ctgacaacaa gtcgggagtt gcagaaatta tggatgctgt atttcagatg attcaacatc
     4441 cggaaatcaa gcacggcaaa cttagaatcc tttttacgcc ggatgaggag atcggcaggg
     4501 gagtggataa ggtcgatatg gttaaattgg ccgcagactt tggttacacg atggatggtg
     4561 agactgtagg ccatgtcgaa gacgaaaact tcaatgctaa agcggccgtg gtaaagatta
     4621 ccggagt
//
LOCUS       DAOQQN010000266         4622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8645_length_4622_cov_4.658638, whole genome shotgun sequence.
ACCESSION   DAOQQN010000266 DAOQQN010000000
VERSION     DAOQQN010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8645_length_4622_cov_4.658638"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1691)
                     /locus_tag="PLG24_09820"
     CDS             complement(99..1691)
                     /locus_tag="PLG24_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ25231.1"
                     /translation="MEKYFNKPILIVALILVTLNVVNAQRDLKSINVLSEKIQDRYSV
                     FIKWKKSNFRNLFINKTDLFPTGFDGRLNGSRIKAFSLLEGEDELLQTVDSTIFYWSG
                     DRDNTEKILFPVMNSLDITINQLLSDPENEFIPPVPLTDSILSLEFEESIGAFVPDDR
                     LVQTRNNQDLTELQLNYEHDGFAWIFDEKAEFEYDNRGNMILAREYDFNGTKWLQDDD
                     QSLEYNSSNRLTATQKIKHDSTTGGVYGQFKHAWSYDDKGRLTEVLNFDWSDAINGWE
                     DDEKELYTYDDLGNLTKAEFFSWNDDSKTWEGTERHHLLYNLNNLKEFVRSENFIGNQ
                     WVEVQQIYYTYDDNNRVVTMEIFHQDSSQWVKSQKAELTYPDINQVDITVLEWDKDAG
                     VYENLIKNWVVYNDLRQPLRIRYQIWDEDKWTYFTFLPNSNFYYEDYGPSGHTSGKER
                     EAEFTVFPSPARDIITVKCENVRVENIRILDIKGRVVRSYQSRLHENIIAIPVSDLTP
                     GTYFVRLEGAGCSGIKPFVVQR"
     gene            complement(2037..3248)
                     /locus_tag="PLG24_09825"
     CDS             complement(2037..3248)
                     /locus_tag="PLG24_09825"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HMZ25232.1"
                     /translation="MVKETYIIDGIRTPIGSFCGTLSQVRTDDLLTHVLRGLAARNTN
                     LDLSAIDDVIIGCANQAGEDNRNVARMCTLMAGYPVTVPAETINRLCASGMSAVVHAH
                     RAIQAGDGNLFVSGGVEHMTRGPWVISKTSTPFGRDARMYDSSFGWRFINPMIQQVYG
                     TEAMGETAENLVEKYGITRERQDVFAFHSQQKVKKAREAGYFKDEILPIEVVKSKREI
                     YTMAEDEFPRPDTTPEILAGLKPAFKKDGSVTAGNSSGLNDGAAALLVASGQAVQEHA
                     LKPLARIVSSSVAGVEPRIMGVGPVPASLRALEKAGLAISDMDIIEINEAFAAQVLAC
                     LDSLKISDNDPRVNPQGGAIALGHPLGMTGSRILLSAALQLQRTGKRFALVTMCIGVG
                     QGYAVIIERAD"
     gene            complement(3261..>4622)
                     /locus_tag="PLG24_09830"
     CDS             complement(3261..>4622)
                     /locus_tag="PLG24_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25233.1"
                     /translation="PAIAAEKYEAIITAHPRVEEYYIKLARLYFDAGLPLKSSDALDR
                     MMQVLGESEEGLYRRFQAEYESGNIDACITTLKRLADIAPENIYYKERLAWAYEYNGQ
                     KDAAVGIYKEILKVDPTNARANIAMAENKTPELAQGEKLSSIMGFVKNPDIPLDRKVN
                     EIIHFLDLYIQSRDTGLVSPLDSVATHLPTLYSGDPKAWALSGDIKLHIGQYLSALVF
                     YDNALAMTRKVYAIFDQKMLILKYLKRYNELRITAETALDVFPNQIGAYINLAYAQVK
                     LGKAQEAIFTLSTAELMIRDTDPHASLIAALMAVAYHDVADKVKEDLYIEKADRLSKN
                     GPQAMMQVATVLGDFDIRPDIAKSKIADTSLQDVTDPFQLATVAMVYFRTKDYTSALK
                     WIEKAIARGGLKYPSITEQAGDILYMAKKAEQAIEYWKQAKKMGVSSSVLDRKISNRT
                     YVQ"
BASE COUNT         1194 a         1078 c          967 g         1383 t
ORIGIN      
        1 tgtcccggga gtgaaacaat ccggggttcc gggatgccga ggttacagtc gtataaatca
       61 atgaaattgg tctgtacgac tgtaacctcg gtattatatc atcgttgcac gacaaatggc
      121 ttgatcccgg aacatcctgc cccctccagt cggacgaaat aggtcccagg tgtcaggtcg
      181 gataccggaa tggcaatgat attttcatga agacgagatt ggtatgatct aacaacccgg
      241 cctttgatat ctaaaatacg gatattttca acccggacat tctcgcattt cacggtaatt
      301 atatccctgg ccggacttgg aaatacggta aattcagctt ctctctcctt accagaagta
      361 tggccggatg gaccataatc ttcataataa aaattagagt ttggcaaaaa tgtgaaataa
      421 gtccacttat cttcatccca gatttgatac cgaattcgta acggctgtct caaatcgtta
      481 tagacgaccc aatttttaat taggttttcg tacactccgg catctttgtc ccattcaagg
      541 acggtaatgt ccacctgatt aatatccgga tatgtcaatt ctgctttttg tgatttaacc
      601 cattgagagc tgtcctgatg gaaaatttcc atagtaacaa ccctgttatt atcatcataa
      661 gtatagtaaa tctgctgtac ctctacccat tgattaccga tgaaattttc agaccggaca
      721 aattctttca ggttattcaa attgtacaat aaatgatgtc tttctgttcc ctcccatgtt
      781 ttggaatcgt cattccatga gaagaattct gctttagtaa gattgcccaa atcatcatag
      841 gtgtacagtt ccttttcatc gtcttcccaa ccattgattg catcactcca atcaaaattc
      901 aggacttctg tcaaacgccc cttatcatca taactccagg catgcttaaa ctgaccgtac
      961 actcctcctg ttgttgaatc gtgtttgatt ttttgtgtag cagtgagcct gttggatgaa
     1021 ttatattcca gtgactgatc gtcatcttgc agccattttg ttccgttaaa gtcatattct
     1081 ctggctagaa tcatatttcc ccgattgtca tattcaaact cagccttttc atcgaaaatc
     1141 caggcaaaac catcgtgttc ataattaagt tgaagctctg ttaaatcctg attattcctc
     1201 gtctgaacca acctgtcatc cggaacaaag gccccaattg attcctcaaa ttccagcgag
     1261 aggatagagt cggtcaaagg cacaggagga atgaattcgt tttcagggtc agaaagtagt
     1321 tggttaatgg ttatatcaag agaattcatc accggaaaca ggatcttctc tgtgttgtcc
     1381 ctgtcaccac tccagtaaaa tatagtagaa tcaacggttt gcagcaattc gtcctctcct
     1441 tctagtaaag aaaaggcctt aattcttgat ccatttagtc ggccatcaaa cccggttgga
     1501 aacaggtcgg ttttgtttat gaaaagattg cggaaattgc tttttttcca cttgataaaa
     1561 acgctatacc ggtcctgaat cttctccgac aaaacattga tggatttgag gtcacgctgc
     1621 gcattaacca cgtttaaggt caccaaaatc aaagcgacaa tcaatattgg tttgttgaaa
     1681 tacttttcca taatattcta tttaaaatga aaaatgaatt ttttatttgc ttagagttat
     1741 ttgaatgatt aaaataatcc gggtaattaa tcgggattgc ttatctatta aaaggtaaat
     1801 aataaaatta aagttccgcc tatatcttgt tatttactta acttaaatca ttgaaattaa
     1861 aaattttcac aagttattta ttctataata aacgtgaaag tcctgagccg gaaatggctt
     1921 gtagggtagg ttatatcttc gatatgtcca taacaaaatg gcattcttgg tttgattctt
     1981 taaggtcatc tatacatgat gacagatcac aacatcgatt attgctgaat tggggatcaa
     2041 tcagcgcgct cgataatcac cgcatagccc tgacctacac cgatgcacat ggttaccaat
     2101 gcaaagcgtt ttcctgtccg ttggagctgt aatgccgcag acagcaagat acggcttccg
     2161 gtcattccaa gcggatgtcc gagtgctatg gcacctcctt gcggattgac tctggggtcg
     2221 ttgtcactga tcttaagact atcgaggcag gcaagaacct gtgcggcaaa ggcttcatta
     2281 atctcgatga tatccatgtc tgaaatcgcc aggccggcct tctccaatgc ccgaaggctt
     2341 gcgggcacag gtccgacacc catgatgcgg ggctccactc cagctaccga ggaagaaaca
     2401 atccttgcca atggtttgag ggcgtgttcc tgtactgcct ggccggaggc caccaaaagg
     2461 gcggctgcgc catcattcaa accggagctg ttgccggcag taacgcttcc atcctttttg
     2521 aatgcaggtt tgagtccggc aaggatttcg ggggtagtat ccggccttgg aaattcatcc
     2581 tcagccatcg tatatatttc ccgcttgctc ttgactactt ctattggcaa aatctcatcc
     2641 ttaaagtatc ccgcttctcg tgcttttttc actttttgct gggagtgaaa ggcaaataca
     2701 tcctgtcgtt ctctggtgat gccatatttt tccaccaaat tctctgccgt ttcgcccatg
     2761 gcttccgtgc catatacctg ttgaatcata ggattgataa atcgccagcc aaagctggag
     2821 tcgtacattc gggcatcccg accaaacgga gtcgatgtct tactgatgac ccagggcccg
     2881 cgtgtcatat gttcgacgcc tccggaaaca aataagttcc cgtctcccgc ttgaattgcg
     2941 cgatgagcat gtaccactgc cgacatgccg gatgcacaca gcctgttgat tgtctctgcc
     3001 gggactgtta ccggatagcc tgccatcagt gtgcacattc tggctacatt tcggttatct
     3061 tctcctgcct gattggcaca acctatgatg acgtcatcga ttgctgagag gtccaggttg
     3121 gtgttccttg cagcaaggcc tctcaaaaca tgtgtcagca gatcgtccgt tctgacctgt
     3181 gacaaggtgc cacaaaagct ccctatcggt gttcttattc catcaatgat gtatgtttcc
     3241 tttaccatga ttcactcatt ttattggaca taggtccggt ttgatatttt gcggtccagg
     3301 acagaagatg aaactcccat ctttttggct tgcttccagt attctatggc ctgttccgcc
     3361 ttttttgcca tatacaggat atccccggcc tgttctgtga tggaaggata cttcaaccct
     3421 ccccgggcta tggctttttc aatccatttc agagccgatg tataatcttt agtcctgaag
     3481 tagaccatcg ctacagttgc caactgaaag ggatccgtca catcctgtag ggaagtatct
     3541 gcaatcttgc tttttgcaat atcaggcctg atatcaaaat cccccaaaac cgttgcaacc
     3601 tgcatcatag cctggggtcc gtttttgctc agccggtcgg ccttctcgat gtagagatcc
     3661 tctttgacct tatcggctac atcatgatag gcaacggcca tcaaagcggc tatcaatgaa
     3721 gcatgcgggt ctgtatcgcg tatcatgagt tcagcagtac ttagtgtgaa tattgcttcc
     3781 tgagctttgc ctaacttcac ctgggcgtat gccagattga tgtatgcgcc aatctgattg
     3841 ggaaatacat ccagcgcggt ctctgcggtt attctgagct cattgtaacg tttgagatat
     3901 ttaaggatca gcattttttg atcaaaaatg gcatagactt ttctggtcat cgccaatgca
     3961 ttgtcataga agacaagggc gctcaggtat tgaccgatat gcagcttgat atcaccggac
     4021 aacgcccatg ctttcgggtc gccggagtaa agtgtcggga gatgtgtcgc cacagaatcc
     4081 aatggagaaa caaggccggt atcccgactt tgaatgtaca gatcaagaaa gtggataatc
     4141 tcattgacct tccggtctaa agggatgtcc ggatttttga caaaacccat gattgagctt
     4201 aatttttccc cttgagccaa ttccggagtt ttattttctg ccatggctat attggcgcgg
     4261 gcattcgttg ggtcaacctt taaaatttcc ttatagatcc caacagcagc atccttttga
     4321 ccgttgtatt cgtatgccca tgccaatcgt tccttgtaat aaatattctc aggggcaatg
     4381 tcagccaatc gtttcaacgt ggtgatacag gcatcgatgt taccggattc gtattcggcc
     4441 tgaaatctac ggtataaacc ctcttcgctt tcaccaagaa cctgcatcat ccggtcaaga
     4501 gcatctgagg actttagagg caaacctgca tcaaagtaaa gtcgggcaag cttgatataa
     4561 tactcctcga cacgaggatg tgccgttatg attgcttcat acttttcagc ggctattgcc
     4621 gg
//
LOCUS       DAOQQN010000267         4612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8675_length_4612_cov_4.577573, whole genome shotgun sequence.
ACCESSION   DAOQQN010000267 DAOQQN010000000
VERSION     DAOQQN010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8675_length_4612_cov_4.577573"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PLG24_09835"
     CDS             <1..352
                     /locus_tag="PLG24_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA ligase (NAD(+)) LigA"
                     /protein_id="HMZ25234.1"
                     /translation="EDPRNIDMLKELEGLGVNMLATNQDHKSLIQVTGHYLSGKTILF
                     TGTLHQMGRKEAQEKAEALGAKNLSAVSANLNILVVGENAGSKLKKAKDLGSVEIWTE
                     QEFLDRLAELQTNS"
     gene            407..1432
                     /gene="pheS"
                     /locus_tag="PLG24_09840"
     CDS             407..1432
                     /gene="pheS"
                     /locus_tag="PLG24_09840"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HMZ25235.1"
                     /translation="MSDTHYSKISEMAEDIEAFPVSNKEDLEAFRIAFLGSKNKLKDL
                     FALMGSVAAEHKKAFGQALNGLKKSAEEKFNSLSASLNNQGESLTSGKMDLTRPGYPT
                     TLGSRHPVSLTLNRIIEIFTKIGFTVEENREIEDEWHNFTALNTPEDHPARDMTDTYY
                     LANDFTRMLRSQTSTVQVHVMESQKPPIRIISPGRVYRNETISARSHCQFHQVEGLYI
                     DKNVSLADMKQTLNYYARAMYGPKARTRLRASYFPFTEPSAEMDVYWGLETEEDRRIT
                     KGTGWLEILGCGMVDPAVLENCGIDPDVYSGFAWGMGIERQAMLKYKITDIRHFFEND
                     IRFLSQF"
     gene            complement(1611..3548)
                     /locus_tag="PLG24_09845"
     CDS             complement(1611..3548)
                     /locus_tag="PLG24_09845"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HMZ25236.1"
                     /translation="MFRRFTSYLHIAFMSILSYGALGQNVDTVDFVYFEEAKHGLIYT
                     KEKVSIFIGEVSFNHKGVYFYSDTVYRSDQLIRAIGNVLIQQGDTLSIFADSLHYNAT
                     TKQCELFGNVSFTKGEQRLYTEKLSYNTETKIAAFDEYGTFTNGPTWVKSKSAVYDTR
                     SEDIVLNEHVFVQDPDFDIKTSKLKYNSRTRLVTFLAPTLVAQKDGTRLYCESGFYNI
                     DKKYGEFVGNPQYQKGKDKAMARKKMIYDGRNEYYFMHGDARYQDSLRYVQGESVKYF
                     QKREYFEVDGGDGFAFLQDSSQMLKGKKIQYDKKKKILKTTGRSRIEDKDNIIEADNN
                     DFNDETGTGMSRGNVILHDKANNSTIHAECVDIDRKKENFKAFGKRAMFTVVNEEDTV
                     HISADTLISFKKDSLTGDTSRIILAYYHVKIFSKSFQGVSDSMYYTTLDSIFRLYRNP
                     VLWSDTTNQYFGDTILIFQKDKKLSSIMLQNKAMIITTPEEVYFNQISGKEIRAIMAD
                     DKIHALTVDGNARTVFYNRNDQEEYTGVNTLDCSHLRAVFDDGELSSIKFYKKNDGKY
                     YPMNKANHAEIQLSGFKWDISRRPVSLQEMRADGANVMMFTLSDKEPDLDTQVVLTMP
                     EEKPVKETSKKKPGINRNKKR"
     gene            complement(3556..>4612)
                     /locus_tag="PLG24_09850"
     CDS             complement(3556..>4612)
                     /locus_tag="PLG24_09850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ25237.1"
                     /translation="ITGLSAALSLREKKPHASILILERGLLPTGASTKNAGFACFGSA
                     SEIWNDVAAYGRQKVVDTVRMRFEGLEMTKRILGSQSIDYVECGGVDVFTEGDHAVYE
                     DCVDNLLEINALAEEATGIPDIYSAHTGNPIGCKGFTHFIRNRAEGSLNTASLYTSLL
                     DLCRQEGVRLLFGLNVVNIDTDFRTVEFDFFDKISCPNIIVTTNGFASRLLPVLDVQF
                     VRNQVLVTERIPGLDLLECVHYNSGFIYFRQVDGRILMGGGRNVLGSAEVTDEMSTTN
                     EAYELLRHVMINHIAVDTIPQVEYHWAGILGVGSDKKPIIEQINPGIFVGVRMGGMGV
                     AIAPVVGRELAELVVNS"
BASE COUNT         1361 a         1033 c          905 g         1313 t
ORIGIN      
        1 cgaggatcca cgcaatatcg acatgctgaa agaattagag ggtttgggag taaacatgtt
       61 agcgactaat caggaccaca aatcacttat tcaggtcaca ggacattatc tgtcagggaa
      121 aactatcttg tttacaggaa ctctccatca aatggggcgt aaagaggctc aggagaaagc
      181 cgaggccctt ggggccaaga atctgagtgc cgtaagtgcc aatctgaaca ttcttgttgt
      241 aggcgaaaat gcaggtagca agctcaaaaa ggcaaaagat ctgggcagtg tggagatttg
      301 gacagaacaa gagtttctgg acagactggc cgaattacag acaaattcct gaaatttcaa
      361 taataacaaa gcaattcctt atttttgcga cataataaag atagcaatga gcgatacaca
      421 ttactctaaa ataagtgaga tggcagagga catcgaggca tttccggtaa gtaataagga
      481 agatctcgaa gcatttagga tagcttttct tggttccaaa aacaagctga aagacctatt
      541 tgccctaatg ggcagtgtgg cggctgaaca taaaaaagct ttcggtcagg cactcaacgg
      601 gttgaaaaaa agtgccgagg aaaaattcaa cagtctttct gcctcattaa acaatcaagg
      661 agaaagcctt acttccggaa aaatggacct caccagaccg ggctatccaa ctaccttagg
      721 atcccggcat cctgtttcat tgacccttaa tcgcatcatc gaaatattta ccaaaattgg
      781 atttacggtt gaagagaatc gcgaaattga agatgagtgg cacaatttta cagcattgaa
      841 cactccggaa gatcatcccg cgcgtgacat gacggatacc tattaccttg ccaatgactt
      901 cacacgcatg ctcagaagtc agacatcgac ggttcaggtg catgttatgg aaagtcaaaa
      961 acctcctatc cgcattattt ctccgggacg cgtttatcgt aacgaaacaa tctctgcccg
     1021 ctcccactgc cagtttcatc aagtagaagg cttatatatc gataagaacg taagtctcgc
     1081 cgatatgaag cagacattga actattatgc cagggccatg tatggtccca aggccaggac
     1141 caggcttcgt gcctcatatt ttccatttac cgagccaagt gcagagatgg atgtttactg
     1201 gggactggaa acagaagaag acaggcggat tactaaaggc accggctggc tggagatact
     1261 gggttgtgga atggtcgatc ccgccgtatt ggaaaattgc ggtatcgatc cggatgtgta
     1321 tagtggtttt gcctggggca tgggtataga aagacaagca atgcttaaat ataaaattac
     1381 cgatatcagg catttcttcg agaatgatat ccgttttttg agccagtttt aagaaaccat
     1441 gcttgaataa gtaaatctat tgtcaagaaa ataattcttc gtttttctag caatattgta
     1501 tttcttagca cttttttctc cggcttatta agccacagac gattccagcg ctaatccagt
     1561 ctcttaattt cgagtttaaa atcagttcta cctgatctaa aaccatcata tcatcgtttt
     1621 ttgtttctat tgatgccggg ttttttcttc gaggtctctt ttaccggctt ttcttcgggc
     1681 atcgtgagta cgacttgagt atccaggtca ggttccttgt cggatagggt aaacatcatg
     1741 acattggccc catcagcccg catctcttga agggagaccg gacgtcggct gatatcccat
     1801 ttaaaaccgg ataattgaat ttcagcatgg ttggctttat tcatgggata atattttccg
     1861 tcatttttct tgtagaattt gatagatgac aactcaccat catcgaaaac cgcacgcaaa
     1921 tgtgaacaat ccaacgtatt tactccggtg tattcttcct gatcattacg attataaaaa
     1981 acagttcgtg cattgccatc aactgtcagg gcatgtatct tgtcatccgc cataattgcc
     2041 cggatttcct taccactgat ttgattgaaa taaacttctt cgggcgttgt aataatcatt
     2101 gccttgtttt gcagcataat ggaagacaat ttcttatctt tctgaaagat gaggatggtg
     2161 tctccaaaat actgattggt agtatctgac caaagtacgg gattcctgta tagtctgaaa
     2221 atgctgtcca acgtcgtata atacatcgag tcactcactc cctgaaagct tttactaaat
     2281 atcttaacat ggtagtaagc cagaatgatc ctggatgtat cgcctgtaag actatccttc
     2341 ttgaaggaaa taagagtgtc tgcactgata tgcaccgtat cttcttcatt aaccacagta
     2401 aacatcgctc gtttgccgaa tgccttaaaa ttctccttct tcctgtcaat atccacacat
     2461 tcggcatgta ttgttgagtt attcgcctta tcatgcaaga tcacattacc acgagacatc
     2521 cctgttcctg tttcgtcgtt gaagtcgtta ttatcagcct cgatgatgtt atccttgtcc
     2581 tcgatacgac tacgtccggt agtttttagg attttcttct ttttatcata ctgaattttt
     2641 tttcccttga gcatctgact ggaatcttgc agaaaagcga agccatcacc tccatcgact
     2701 tcgaaatatt cgcgcttttg aaaatatttc accgattcgc cctggacata acgcaaagag
     2761 tcttgatatc gagcatcccc atgcatgaaa tagtattcat ttctgccatc atagatcatt
     2821 tttttccggg ccatggcttt atccttccct ttctgatatt gtggattccc gacaaactca
     2881 ccatactttt tatcgatatt gtaaaaaccg gactcgcaat acaatcgcgt cccatccttt
     2941 tgggcaacaa gtgtgggagc caaaaaagta accagtctcg ttctggaatt atatttcaat
     3001 ttactggttt tgatgtcgaa gtcaggatcc tggacaaaaa cgtgttcatt gagaactata
     3061 tcttccgacc tggtatcata gacggcggat ttacttttaa cccaggtagg gccattggtg
     3121 aatgtgccat attcgtcaaa ggcagcaatc ttagtttctg tattgtagga taacttttca
     3181 gtatataagc gctgctcccc tttggtaaaa gagacattac caaacagctc acattgttta
     3241 gtagtagcat tgtagtgaag ggaatcggca aatatgctca aggtatcacc ttgctggata
     3301 agtacgttac ctatagcccg aatcagttga tcggaacgat ataccgtgtc agaataaaaa
     3361 tatacaccct tatggttaaa cgagacttcg ccaataaaga tactgacctt ctctttggta
     3421 taaatcaatc catgtttggc ctcttcaaaa tagacaaaat caacggtatc gacattttga
     3481 cccaatgcgc cataagacaa aatgctcatg aaggcaatat ggagatagct ggtaaacctt
     3541 ctaaacatca aaatcttaag aatttacaac taattcagcc agttcacgac caacaacagg
     3601 agcaattgca acgcccatac cacccatacg gacaccgacg aagattccgg gattgatttg
     3661 ttcaataatt ggttttttat ctgacccgac accaagaata cctgcccagt gatattccac
     3721 ttgaggtatt gtatcaaccg cgatatgatt tatcataaca tggcgcaaca attcataagc
     3781 ttcattggtc gtagacatct cgtctgtcac ttcggcacta ccgaggacat ttcgcccacc
     3841 gcccattaat atccttccgt caacctgcct gaaataaata aaaccactat tataatgaac
     3901 acattctaac agatcaagac ccgggattct ttccgttaca agaacctgat tccgaacaaa
     3961 ttgaacgtcc aaaacaggca acaaccgtga cgcaaatcca ttggtagtca caatgatatt
     4021 cggacaagaa attttatcaa aaaaatcaaa ctcaacggtc cgaaaatccg tatcaatatt
     4081 cactacatta aggccaaaca acaacctgac gccttcctgt ctgcaaagat ccagcaaaga
     4141 ggtgtacaag gaagctgtat tgaggctacc ttccgcccta ttccggataa aatgtgtaaa
     4201 acccttacat cctataggat tgccggtatg tgcagagtat atatcaggga tgccggttgc
     4261 ctcctcagct aaagcattta tttctaaaag attgtccaca caatcctcat atactgcatg
     4321 atcgccttct gtgaacacgt ctaccccacc acattctaca taatcgatac tttggctgcc
     4381 caatatcctt ttggtcattt cgaggccttc aaatctcatt cgcacggtat ccacaacctt
     4441 ttgacggcca tatgcagcca catcattcca tatttccgaa gcacttccga aacaggcaaa
     4501 accggcattt tttgtactgg ctccggtggg caataatcct ctctctaaaa taagaatgga
     4561 tgcgtgcggt tttttttcac gcagagaaag tgcggcagat aatccggtaa tt
//
LOCUS       DAOQQN010000268         4576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8772_length_4576_cov_4.210131, whole genome shotgun sequence.
ACCESSION   DAOQQN010000268 DAOQQN010000000
VERSION     DAOQQN010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8772_length_4576_cov_4.210131"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="PLG24_09855"
     CDS             <1..953
                     /locus_tag="PLG24_09855"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HMZ25238.1"
                     /translation="NLPYVMAVEKMGGIEVPDRAKIIRIMLCELFRISSHLVWYGTFA
                     QDLGMMTPVFLTFYDREKLFTIIEAICGDRMHPNWFRIGGVAQDLPTGWEQLVEDFVK
                     YFPPRLKEYDKMVMKNRIFKARTVGIGVYNTDEAIEWGVTGPGLRATGLGWDMRKQQP
                     YSGYDQFDFEVPVAVNGDCYDRAWVHVEEMRQSIRIIEQCLKNMPAGPYKSTHALTTP
                     PVKERTMYDIETLINHFVNISWGPVLPPTEGFCCIESSKGNNGYYLVSDGSTASYRTR
                     IRTPSFPHIQFVPEYSKGYTIADLMAILGSVDFVLSDIDR"
     gene            972..1451
                     /gene="nuoE"
                     /locus_tag="PLG24_09860"
     CDS             972..1451
                     /gene="nuoE"
                     /locus_tag="PLG24_09860"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HMZ25239.1"
                     /translation="MTTEQVISNEARQEIESLFHHLPHKQAACIDALKVVQKYNRWVS
                     DEALNELAPILEMSPHEIDSVATFYNIIFRRPVGRHVIFLCNSVSCYIMGYDKIHEAI
                     KQKLGIDFGQTTPDDRFTFLPIPCLGTCDHAPALLVDEDLHRDLTPEQLDEILAKYE"
     gene            1458..2735
                     /gene="nuoF"
                     /locus_tag="PLG24_09865"
     CDS             1458..2735
                     /gene="nuoF"
                     /locus_tag="PLG24_09865"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HMZ25240.1"
                     /translation="MERPLTEHIKKDGTALSLKEYEAVGGYQGLRAALKMSPSEVLNV
                     VKDSKLRGRGGAGFPTGMKWSFVPMGDNAPKVKYLVANGDEMEPGTFKDRLLIEGNPH
                     QLIEGMIISAYTIGASMSYIFLRWAYHLAAKRVLIALDEAYKAGYLGKNILGSGYSLD
                     MDLHTSVGRYICGEETALLNALEGKRANPRNKPPFPQVSGLFGKPTIVNNIETYCNIP
                     HILAKGPQWYISLSNSEGGGGTKLYGASGKVKRPAIWELPLGTTLGEIMEEHAGGMQD
                     GYKVRGLLPGGASTDYLTPKHYNIKMDFEEVAKAGSRMGTGTIIVMDDKTCPIGMCLN
                     LERFFAQESCGWCTPCREGLPWTAKILLALEEGRGVPEDIEQLEKQTRYMAPGNTFCA
                     LAPGAMEPLQSALKYFREDFEEHVHNKHCKYHH"
     gene            2758..>4576
                     /gene="nuoG"
                     /locus_tag="PLG24_09870"
     CDS             2758..>4576
                     /gene="nuoG"
                     /locus_tag="PLG24_09870"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HMZ25241.1"
                     /translation="MAKVYIDNVEYEVPDGKNLLEVCLSLGKDITYFCWHPKMHSVGA
                     CRQCAVTVYRDENDTRGRLGMACMMDAVDGTRISIQDESSKNFRAGNIESLMINHPHD
                     CAVCDEGGECHLQDMTVMSGHNYRNYRFEKRTHINQDLGPFINHEMNRCIQCYRCVRF
                     YNDYAGGEDFGVFASSGRVYFGRSEDGTLENEFSGNLVEVCPTGVFTDKTLRQHYTRK
                     WDLTSAPSICQGCSVGCNILTSERYGKVRRVMTRFNGDVNGYFLCDRGRFGYEYANDE
                     KRLTRAAQKDNSSGTNAFTSIIQHLGTKPGMIGIGSPRASLESNFMLKQLVGAENFYS
                     GISGREKEVVDAALDVLANGKVKTPTLAEIENYETVFVLGEDVTNTSPMIALALRQTS
                     KTLPRQKAYALRIQTWNDAAIREVVQDDKGPFYIATPGRTKLEEIATSATHGDTKTIL
                     DIGNEVAELIKSGKADASNESFAHQTASALLAHDKPLIVSGAGMMSSLIVRCAAQIAT
                     ALKEAGKDVGLVLSVPEANSIGLAMIGSAGSFEDAMQRVEEDRRLTAVVIETDLYYRT
                     DRQSASSFLNNCNKVILLDSLENDTTPEVDYVLPAGTFAE"
BASE COUNT         1295 a          925 c         1180 g         1176 t
ORIGIN      
        1 ataacctgcc ttatgtaatg gcagtggaga agatgggcgg tatcgaagtg cctgatcgtg
       61 ccaaaattat ccgcatcatg ctctgtgagt tgttccgtat atcaagccac ctggtttggt
      121 atggtacctt tgctcaggac ctcggtatga tgacacctgt cttccttacc ttctacgaca
      181 gagaaaagct gtttaccatc atcgaggcca tatgtggtga caggatgcac cccaattggt
      241 tcagaattgg aggtgtggca caggatctgc ctaccggatg ggagcaattg gttgaggatt
      301 ttgtcaagta tttccctccg agattgaagg aatatgacaa gatggtgatg aaaaacagaa
      361 tattcaaggc acgtaccgtg gggattggtg tatacaacac agatgaagca atcgaatggg
      421 gagttactgg accggggttg cgggctacag gccttggatg ggacatgaga aaacaacagc
      481 catactccgg atacgatcag tttgactttg aggtcccggt agctgtcaac ggtgattgtt
      541 atgaccgagc atgggtacac gtggaagaaa tgcgccaaag catccgtatt attgagcaat
      601 gtctaaagaa tatgcctgca gggccatata agtccaccca tgcactgact acgccacctg
      661 tcaaggagcg tactatgtat gatatcgaaa ctttgatcaa ccactttgta aatatttcat
      721 gggggccggt tcttcctccg acagaggggt tctgttgtat tgagtccagt aaaggcaata
      781 atggatatta ccttgtgagt gatggaagta cggcttccta caggacgaga atccgcaccc
      841 cgagcttccc gcatattcag tttgtgccgg aatacagcaa aggttatacc attgctgacc
      901 tgatggctat tctgggaagt gttgactttg tattatcaga tattgacaga tagtaaaaat
      961 tattgattta aatgacaaca gaacaagtta tatccaacga ggcgagacag gagattgaaa
     1021 gtctgtttca ccatctgcca cacaaacagg ctgcttgtat cgatgcgctg aaagtcgtgc
     1081 agaaatacaa tcgttgggta tctgacgaag cattaaatga actggctccg atcttggaga
     1141 tgagtcctca cgagatagat agtgtagcga ctttctacaa tataatattc agaagaccgg
     1201 tgggcaggca tgtaatcttt ctgtgcaaca gtgtaagttg ctacatcatg ggttatgata
     1261 agatacatga ggcgatcaag caaaaactgg gaatagattt tgggcagact acacccgatg
     1321 atcgctttac cttcctgccc atcccatgcc ttggcacttg tgatcatgcc cctgcactgc
     1381 tagtcgatga ggatctgcac cgcgacttaa caccggagca attggatgaa attctggcaa
     1441 aatatgaata acgaatgatg gaaagacctc tgaccgaaca tattaagaaa gacggaacag
     1501 ccctgtcact caaggagtac gaagcagtgg gcggttacca gggattgcgg gcagccctta
     1561 agatgtcacc atctgaagtg ctcaatgtgg tgaaggattc gaaattacgt ggacgtggtg
     1621 gagccggatt ccctacaggt atgaaatgga gttttgtacc gatgggagat aatgccccaa
     1681 aggtcaagta tcttgtagct aatggtgacg agatggagcc gggcaccttt aaagataggt
     1741 tgttgataga aggcaatccc caccaattga ttgaagggat gatcatcagt gcctatacca
     1801 ttggggccag tatgagttat atcttcctgc gatgggctta tcatctggca gctaaaagag
     1861 ttttgattgc attggatgaa gcttataaag caggttacct gggcaaaaat atccttggta
     1921 gtggttattc cctggatatg gacctgcata cgagcgtcgg ccgttacatt tgtggtgaag
     1981 aaacagccct tttgaatgct ttggaaggga agagagccaa ccctaggaat aagccgccct
     2041 tccctcaggt gagcggactg tttggcaaac ctaccatcgt caataacatc gagacatatt
     2101 gcaatattcc ccatattctg gcgaaggggc cacaatggta tatttccctc agcaattcgg
     2161 agggaggtgg cggaacaaaa ctttatggtg cgagtggcaa agtcaagcga ccggccatct
     2221 gggagttgcc tctggggaca acactcggtg aaattatgga agaacatgca ggcggtatgc
     2281 aggacggata taaagtaaga ggattactac ccggcggtgc gtctactgat tacctgactc
     2341 ctaagcatta taacattaag atggactttg aggaagtggc caaggccgga agtcgtatgg
     2401 gtacgggaac catcatcgtc atggatgaca agacttgtcc cataggcatg tgtcttaatc
     2461 ttgaacgttt ctttgcccag gaatcctgcg gctggtgtac accttgtcgg gaaggcctgc
     2521 cttggacagc taagatactt ctggcactgg aagaaggtag aggtgtaccg gaagatatcg
     2581 agcagcttga gaagcaaaca cggtatatgg ctccgggtaa cacgttctgc gcccttgcac
     2641 ccggtgctat ggaaccactt caaagtgcac tgaaatactt cagggaagat tttgaagagc
     2701 acgtccacaa caaacattgt aaatatcatc attgattgat atcataaaat aagagatatg
     2761 gctaaagttt atatcgataa cgtagaatac gaagttcctg atggtaagaa tttgctggag
     2821 gtgtgcttat ccttgggtaa ggacatcacc tatttttgct ggcacccgaa gatgcattcc
     2881 gtaggtgcct gtcgtcagtg cgccgtaacc gtttatcgcg atgaaaacga tacccgtggg
     2941 agattgggga tggcatgtat gatggatgct gtggatggta caagaatttc cattcaggat
     3001 gaaagctcaa aaaacttcag ggccggtaat attgaatcat tgatgattaa tcacccgcac
     3061 gattgtgcag tgtgcgatga aggcggtgaa tgtcatcttc aggatatgac ggtaatgagc
     3121 ggtcacaact atcgcaatta ccgttttgag aaaaggactc atatcaatca ggatttaggg
     3181 ccctttatca atcatgagat gaatcgctgc atccaatgtt accgatgtgt tcgtttctat
     3241 aatgactatg ctggtggtga ggacttcggt gtttttgcgt ccagcggtag agtttatttt
     3301 gggcgcagcg aggatggaac cctcgaaaat gaattcagtg gaaatctcgt ggaggtatgc
     3361 cctacaggag tgtttaccga taaaacacta cgtcagcatt ataccagaaa atgggatttg
     3421 accagtgccc catccatctg tcaaggctgt tcagtaggat gcaatatcct tacttctgag
     3481 cgttatggca aagttcgtcg agtgatgacg cgcttcaatg gagatgtaaa tggttacttt
     3541 ctttgtgatc gtgggagatt cggatatgaa tatgccaatg atgaaaaacg tttgacaaga
     3601 gctgcccaaa aggataattc ttccggtacc aatgccttca catcaattat acaacatttg
     3661 ggtacaaagc cgggtatgat tggtattgga tcgcctcgag cttcccttga atccaatttc
     3721 atgctgaagc agctggtagg agcagagaat ttctattctg gtatttccgg aagagagaag
     3781 gaagttgtgg atgctgctct ggatgttctt gccaatggaa aagtaaagac gcctactttg
     3841 gcagagattg aaaattacga aaccgtattt gtattgggag aagatgttac caatacatct
     3901 ccaatgatag cattggcatt acgccagact tccaagaccc ttccacgcca aaaggcctat
     3961 gcactcagga ttcaaacctg gaatgacgca gcaatccggg aagtagtaca ggatgacaaa
     4021 ggaccattct acatagccac gcccggaaga acaaaacttg aagaaatagc aacttcagcc
     4081 acacatggtg atactaagac aatattggac attggaaatg aggttgctga gttgattaag
     4141 tccggtaagg ccgatgcgtc aaatgaaagc tttgctcacc aaactgcttc agcattatta
     4201 gcgcatgaca agcctttgat agtatcgggc gcgggaatga tgagcagttt aatcgtgcgc
     4261 tgtgctgctc aaatcgcaac agctctgaaa gaagccggta aggatgtggg gcttgtcctg
     4321 tctgtacctg aggccaactc cattggatta gctatgatcg gatcagccgg aagctttgaa
     4381 gatgccatgc aaagagtgga ggaagacagg agactgacag cagttgttat agaaacagac
     4441 ttgtactata ggactgatcg gcagtcagct tcttcattcc tgaataattg caacaaggtc
     4501 attctccttg attctttgga aaatgatact acaccggagg tcgattatgt cttaccggcg
     4561 ggaacctttg cagagt
//
LOCUS       DAOQQN010000269         4568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8789_length_4568_cov_5.822070, whole genome shotgun sequence.
ACCESSION   DAOQQN010000269 DAOQQN010000000
VERSION     DAOQQN010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8789_length_4568_cov_5.822070"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLG24_09875"
     CDS             complement(<1..575)
                     /locus_tag="PLG24_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25242.1"
                     /translation="MIKFTPYLLILLLISCTDPDTPPQKTQKQITDFVDAYYTSSQYE
                     TDDQSRVSLLRAVKGTDLPYVGLECPTLQRPYIAHGELVFTNQPNANFKLLDRQNDDP
                     YSLPEEQTIVFRKMPGTDWEAIISGWGNYYLGFAGINDVRALRPDLYFGMKVPVKYYE
                     LGFFHVRYDREADGRGKAKLWFNGDYFGEVV"
     gene            complement(812..1219)
                     /locus_tag="PLG24_09880"
     CDS             complement(812..1219)
                     /locus_tag="PLG24_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25243.1"
                     /translation="MGLTLFLFGLAVGLFVQNMSNPRMALSAHLEGVMNGMFLVILGL
                     IWNQLILSSTALKTTFWLVIYGTFVNLIAVIIAAMSGNGKMMPIAGGQEGTRAIEGLI
                     SFLLISLSLCMLAVCIIVLFGFYKHMKQKTVEQ"
     gene            complement(1416..1802)
                     /locus_tag="PLG24_09885"
     CDS             complement(1416..1802)
                     /locus_tag="PLG24_09885"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HMZ25244.1"
                     /translation="MKKDTIEANDEETTRLTVERFNSAFNRHDVDAIMSLMTANCVFE
                     NTNPPPDGLRIEGEKAVREYWMKFFANNPDAYFEEEELIVTGNRCVVRWIYRKTKDGK
                     PWHLRGVDVFKVENGKVAEKLAYVKG"
     gene            complement(1821..2201)
                     /locus_tag="PLG24_09890"
     CDS             complement(1821..2201)
                     /locus_tag="PLG24_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMZ25245.1"
                     /translation="MEKTTKNSKMAEWKNFNNPDEVREFPKGRLELVNIGGATIGRGV
                     FEPGWRWATSVQPIANTHSCEAPHFQFHVAGILHVVMDDGSEFDLKPGDVSLLPPGHD
                     AWTVGDETVVVIDFQGMIDYAKHL"
     gene            complement(2286..3206)
                     /locus_tag="PLG24_09895"
     CDS             complement(2286..3206)
                     /locus_tag="PLG24_09895"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ25246.1"
                     /translation="MGKQITSAKRKKEILNIETLEMFERLINRDSKPYALDMLVMPGT
                     LEDKHKNCDAIPALRRQFNLIYLLLEGEHDVKLGADHLVLNPNDLVIVPENMLYASDH
                     IKNCKGYCIHFKPEFLKPQLTRPIHEEFPYFHFDAPHIINLNKKESKIIQQAFRNILE
                     EYNRPSPEKDFLLCNLILILLLRVREIYRIRVKELKKNISRQEQLANSFRLLVEKKFI
                     EKRTVSEYAEQLHISPKHLSDVVSETFGRSPLQIIHDILLLEAKVQLKATDKSVSEIA
                     YYLNFDDQPHFTNFIKKRTGFSPQELRKKL"
     gene            complement(3389..3913)
                     /locus_tag="PLG24_09900"
     CDS             complement(3389..3913)
                     /locus_tag="PLG24_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25247.1"
                     /translation="MKKLVQIILSFIAILASDQVQAQVRYSLKAETGYLNYRNNTIQV
                     DPGPDWKGYYLNGENGIDISVVNGIDIKNRFFAGLGLGYVNFEGINGLLLFANLEFIP
                     LKTGLSPVVDMKFGYSHIRNQYANGTGTGFGELCGGLNYRLSDSFNIFAESGISLTQQ
                     SWFIPFRLGIRLSM"
BASE COUNT         1353 a          924 c          816 g         1475 t
ORIGIN      
        1 ctcacgacct ctccgaaata atcaccgtta aaccagagtt ttgcttttcc acggccgtca
       61 gcttcccggt cgtaccgcac atgaaagaag cccaattcat aatactttac cgggaccttc
      121 atgccaaagt aaagatcagg ccggagagcc cggacgtcat taatcccggc aaagcccaaa
      181 taatagttgc cccacccgct gatgatcgcc tcccagtctg tgccgggcat cttcctgaat
      241 acaatggttt gctcttcggg aagggaataa ggatcatcgt tttggcggtc cagtagctta
      301 aaattagcat taggctgatt ggtaaacacg agttcaccat gggcgatata cggtctttgc
      361 aaggtaggac actccaggcc gacatagggc aagtctgttc ctttcactgc ccgcaacaga
      421 gagaccctgc tctgatcatc tgtttcatat tgagatgacg tatagtaggc atctacaaag
      481 tccgtgattt gcttttgagt tttttgtggc ggagtatcag gatctgtaca ggaaatcagt
      541 agaagaatca agaggtaggg ggtaaatttt atcatctgaa cttgaattgc gaggattcag
      601 gttctaaaat aacctacttt ttttaaatct aaggaaaata ttgctgggaa aatgattacg
      661 attcattccg gccttacctg agaactaaaa atcgggaaaa tatgaaggaa gtgtcggaga
      721 gaatattcta gtacctgaat gccggctgca ggagaattca ttgtgtcgtt atgtgtgtat
      781 catgtataat ggttgagaca aatgttttga atcattgctc gacagttttt tgtttcatgt
      841 gtttataaaa tccaaataaa actatgatgc atactgcaag catgcataaa gaaagagaaa
      901 tgagcaaaaa tgaaatcagt ccttctatcg ctcttgttcc ttcttgtcca ccggcaattg
      961 gcatcatttt tccatttcct gacatggcag caataattac tgctataagg tttacaaatg
     1021 ttccataaat taccaaccag aatgttgttt ttagagctgt tgaagaaagt atcagttgat
     1081 tccagataag tccgagtatt actaaaaaca taccgttcat tacaccttca aggtgagctg
     1141 aaagagccat acgaggattt gacatgtttt gaacaaacag tccgacggca agaccgaata
     1201 aaaaaagagt caaccccaaa aaaatcagtt tgtctccttg ttttgattga atatttttat
     1261 caatttatac tatattacta tgctttaaaa aaccaacctt ttaataaaaa agtagcattt
     1321 gtcatttgaa tgtaaagttc aaataaggat atgaaaatta aatgtctctg atgcatattg
     1381 cagatcagaa tgctgttgga aaaatacaga ataatttacc cttttacata tgccagtttt
     1441 tctgccactt ttccattttc aactttaaaa acatccacgc ctctcagatg ccagggcttc
     1501 ccatctttgg tctttcgata tatccaccga acaacacatc gatttccggt gacaattaat
     1561 tcttcttcct caaaataggc atcagggttg ttagcaaaaa acttcatcca atattctctg
     1621 accgcctttt cgccttcaat ccgcaatcca tccggtggcg gattggtgtt ttcaaagacg
     1681 caatttgcag tcatcagact catgatagca tccacgtcat gccgattaaa tgccgaatta
     1741 aatctttcaa ctgtcagccg tgtcgtttct tcatcatttg cttcaatagt atcttttttc
     1801 atcccaggtc attaaaattt ttataaatgt tttgcataat cgatcatacc ctgaaaatca
     1861 attacaacaa ccgtttcatc tcctacagtc catgcatcat gaccgggagg caataaagaa
     1921 acatcgccgg gtttcagatc aaactcactt ccatcatcca ttactacatg caggataccg
     1981 gccacatgga actggaagtg aggggcttca caactgtgtg tatttgcaat gggctgcaca
     2041 gaggtagccc atctccatcc tggctcaaaa acaccacgcc ctatcgtagc tccgccaata
     2101 tttaccaatt caagcctgcc tttcggaaat tcacggactt catcaggatt attaaaattt
     2161 ttccattccg ccatcttaga attttttgtt gtcttttcca ttatatttaa ttttaaaatg
     2221 atagcacaaa gaaaaacaac aatcttgccg tctgattgta aattattcgg caagacatgt
     2281 ataaatcaga gcttctttcg cagttcctgc ggtgaaaatc cggtacgttt tttaataaag
     2341 ttagtaaagt gcggctggtc gtcaaaattc agataatatg caatttccga aacggatttg
     2401 tcagttgcct tcaactgaac ttttgcttca agcaacaata tatcgtgaat gatttgcagc
     2461 ggagatcgtc caaaagtctc acttaccaca tcagaaagat gtttagggct aatatgtaac
     2521 tgttctgcat attcactaac tgtcctcttt tcaataaatt tcttttcaac caacaacctg
     2581 aaactattgg ctaattgttc ttgtctgctt atgttctttt ttaattcctt tacccgaatc
     2641 ctgtatattt cgcgtactct caacaacaaa atgagaataa gattgcaaag caagaaatct
     2701 ttttccggcg aagggcgatt gtattcttca agaatgtttc tgaatgcttg ctgaataatt
     2761 ttactttcct ttttattcaa attaatgatg tggggtgcat caaaatgaaa ataggggaat
     2821 tcctcatgaa taggccgggt taattgaggt ttcagaaact ccggtttaaa atgaatacaa
     2881 tatcctttgc aattttttat gtggtcactt gcatacaaca tattttcagg aacgattaca
     2941 aggtcattag gatttaacac taaatggtcg gctcctaatt ttacatcatg ctctccttcc
     3001 aataataaat atatgaggtt aaactgtctt ctaagggcag ggatggcatc acaatttttg
     3061 tgtttgtcct caagcgtacc gggcatgact aacatgtcaa gcgcatacgg tttagaatcc
     3121 ctgttgatca gtctttcgaa catctcaagt gtttcaatgt tcagtatttc cttttttctc
     3181 ttcgctgaag ttatttgttt gcccatttgt gggtggtatt tacacgttac cttgggttta
     3241 tgcgacgttg tttgctgatc ttttttggat attttaaatt aacaacgctt aagggcttat
     3301 tttttcgata aagataccgt ttttataaat ataaattata aaattaaatg aattttaaac
     3361 acttttaaat ttcgatataa ctcccgattt acatacttag ccgtatgcct aatctaaaag
     3421 ggataaacca cgattgttga gtcagcgaaa tgcctgattc agcaaaaata ttgaagctgt
     3481 cagaaagtct gtaattaagt ccgccgcata gttcgccaaa tcctgtcccc gtaccgtttg
     3541 catattggtt acggatatga ctatatccga atttcatatc tacaacgggg gataaccctg
     3601 tttttaaggg tatgaattcc aggttggcga aaaggagaag gccgtttatt ccttcaaaat
     3661 ttacatatcc gagtccaagg cctgcaaaaa atctgttttt gatatcaatg ccgtttacga
     3721 cacttatatc aataccattc tctccgttta aatagtatcc cttccagtcc ggaccgggat
     3781 ccacctgaat cgtgttattt cgatagttta aatatcccgt ctctgctttt aatgaatatc
     3841 ttacctgtgc ctgcacctga tctgatgcca ggattgcaat aaaggaaagg attatttgaa
     3901 ctaatttttt catatttcta aaaggaaaga acttaattta ttctgccgga tcatagcttg
     3961 tagaccttac tctgttgaag tcaataccgg tgaagctaca aaaagttatg tctttaaagg
     4021 atcaatttat tttttcaacc cagggaattc attaattata atccccggaa agcttctcca
     4081 atgttaatga cgtaactccg tgcaatttgt ttgaaatttc taaatattat ttttcaatca
     4141 ccgtcttcga aactctgaat gataacttgc agcctgtcac tgctttgttt gtctcaatat
     4201 ttttttcttc ggcttcctta tttttgaatc tttttgaaac tcaaagagat ctttcatttg
     4261 atcaaagaaa tcaaaaattt tatcacaaga cctcgcgatt tttggattat tcatactatt
     4321 ggctgtgttg ctttaactta ccgggagtac atactattca gctgcgacct ctcaggcagg
     4381 ttatgaaagg ccggggattg gcaaagaatt cttgatgcag atgttcaatt ctttttaccg
     4441 attatccaaa aagatttacc tttgaggcgt gaataataaa acacatccca atcccgaaac
     4501 aatggtcgaa gtaaagctat tctcctgcgc tgcctcagag tatttatcgg aaaaaatcag
     4561 cgattatt
//
LOCUS       DAOQQN010000270         4554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8835_length_4554_cov_6.841965, whole genome shotgun sequence.
ACCESSION   DAOQQN010000270 DAOQQN010000000
VERSION     DAOQQN010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8835_length_4554_cov_6.841965"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLG24_09905"
     CDS             <1..236
                     /locus_tag="PLG24_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25248.1"
                     /translation="LKSPEYYATTNNDDYFGFKDKMERIDRGANAISGFIGNQANIVK
                     NEGLFGFAREYDQSGNPDGFSLRIAGLEFSTAK"
     gene            275..1624
                     /locus_tag="PLG24_09910"
     CDS             275..1624
                     /locus_tag="PLG24_09910"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ25249.1"
                     /translation="MKQNIFCIILILGSIYAQAQNNYTQLPRINKVVLNDNSNVTIIE
                     ENADQSGISVTSNKKPGYRVKEGVLYIDDQSIAKIAINNLNQIEANDVSVVTLNGGFS
                     KGLEVIANDATRVKINGNLDVVVIEANDASYITLDGKANSLNVKTNDASKVDGSMIAK
                     HLFAKSFDVSRIVVYGDKYVDTQVNDHSNIEILKNPSTKSTVSDSVIVEEETIRHEMD
                     DQFDGVGKDIEKWNKKKIKWRPNQRVWSGFEMSVTGLSNDLFDFQPNELHEQWAIEQP
                     SVSFHINILEHKFRLGTDYVKFLSGLGMQWDVLRLKKDVTLQNSRDKIQLVDSQYGTD
                     LKKNNLILGQIQIPLLLNFNTKPGSKYNFHIDGGVVVGYRFKQVQKQEIVVDYKTSID
                     QNIKSQFHQNPFDFSATLRLGVGEWSVFGMYDLTSLYKKNEGPQLKVWNIGVTIIPF"
     gene            complement(1723..2145)
                     /gene="mscL"
                     /locus_tag="PLG24_09915"
     CDS             complement(1723..2145)
                     /gene="mscL"
                     /locus_tag="PLG24_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HMZ25250.1"
                     /translation="MGFLKEFKEFATKGSVMDLAVGVIIGGAFGKIVDSIVNDLIMPI
                     VGIVFNADFSNLYIPLSDKITSGLPLDKAKELGPVFAWGNFITVAINFIILAFIIFMM
                     VKAMNKLRNPEPAAPAGPPPPSPTETLLAEIRDELKKR"
     gene            2356..>4554
                     /locus_tag="PLG24_09920"
     CDS             2356..>4554
                     /locus_tag="PLG24_09920"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ25251.1"
                     /translation="MKCPLLFLTLFICNLNVFSQNSRIDGFVADELNGYAIQNATVTI
                     MRTSDSLLVNYSRTKTDGSFSFKSLIPGHYDLFISYPDYLGYATDIELEPGKDFLLDT
                     VYLIKNSQLLKEITIVDGSKIKFKGDTIQFLADSFRVGENANVEDLLKKLSGIKVNSK
                     GEITAFGEKVEKVFVDGEEFFGSDPTIATRNIQAKAVDKVQVFDKTSTMEELTGIADG
                     EKFKAINLTLKEEYKKGYFGKASAGVGLDPVYYDGSLMLQGYTAKSKISAYGIGSNTG
                     TVSLDFNDYGKYMNSSAGSVSFDGNMTTISFSSEDDFSWDGRYDGEGLPKSLAGGASF
                     STKLAKDKIRINANYGYSDIQLKKNEKNISTNYLPDDGFVKDDSQFTDSRIIKHSALF
                     KVEADLDSLTTLIWNNGASTRKTNNYKEVHTRQNDLEQNPITEINRTLTNKNTGNSIN
                     SELTLTRKFKKKHRLLVINGSGNYSENEGSSYLNSRNDYFTLNQFQQINQLVDKGETN
                     FSYQAKATYSEPLGKKWTGQINVYTSNVANHSNKLANEFDEITQQYSKFVDSLSNDFD
                     YRINSNGGGASINYKGEKYDFRFGTDVDYSNTIRKNKIDSSSIIDNALRWIPRANFTY
                     KFNRASRISMNYYGSTRQPSINQIQPVRDNSDPTVLIKGNPALKQSYTQSISMYYNFW
                     KALSDVGLWSYANFSNTFNEIVSNTIIDEARRSITTFTNVNGGYNGNVGFN"
BASE COUNT         1550 a          748 c          881 g         1375 t
ORIGIN      
        1 aattgaagtc tccggaatat tatgcaacca ccaataacga tgactatttt ggtttcaaag
       61 acaaaatgga aaggatagat cggggtgcca atgcgatttc cgggtttatc gggaaccagg
      121 caaatattgt aaaaaatgaa ggattgtttg gctttgctcg ggaatatgat caaagtggca
      181 atccggatgg gtttagtcta agaattgcag gcttagaatt cagtaccgca aaataatttt
      241 cctcaaatat ttattaacca ttgaatatat taaaatgaag caaaacattt tttgcataat
      301 attgatattg ggttcaatat atgctcaagc tcagaacaat tacactcaat tgcccagaat
      361 caacaaggtt gtactaaatg ataactcaaa cgtgacaatc attgaagaga atgctgacca
      421 atccggtatt tcggttacat ccaataaaaa accgggatat agagttaagg aaggtgttct
      481 gtatattgac gatcaatcca ttgccaagat cgcaattaac aatttgaatc aaatagaagc
      541 aaatgatgtc tctgttgtaa cccttaatgg tggattttcc aaaggcctcg aagtcattgc
      601 caatgatgcg accagagtta aaatcaacgg caacctcgat gtagttgtta tcgaagccaa
      661 cgatgcttct tatatcacat tggatggtaa agctaattct ttaaatgtta aaactaatga
      721 tgcgtcaaag gtggatggaa gtatgatagc caagcacttg tttgccaaat cttttgatgt
      781 ttcgaggatc gtagtttatg gcgataaata tgtggatacc caagtaaatg atcattccaa
      841 tattgaaatc ttgaaaaacc cttcaaccaa atctacagtt tctgattccg taattgttga
      901 agaagaaacc atccgacatg aaatggatga tcaatttgat ggggttggta aggatattga
      961 aaaatggaat aagaaaaaaa tcaaatggcg cccgaatcaa cgagtctggt ccggatttga
     1021 gatgtcagta accggtttgt ctaatgattt gtttgatttc cagcccaatg aattgcatga
     1081 gcaatgggct atagaacaac cttccgtttc ttttcatatc aatattctgg aacataagtt
     1141 cagactcgga actgattatg tgaagtttct atcagggttg gggatgcaat gggatgtgct
     1201 cagacttaaa aaagatgtca cactgcagaa ttccagggac aaaatacagc tagtcgattc
     1261 tcagtatggc acagatttaa agaaaaacaa ccttattttg ggccaaattc aaattccact
     1321 gctgttgaat ttcaatacca agccgggcag taaatataat tttcatatcg atggtggagt
     1381 agtagtagga tatagattca agcaggttca aaagcaagag atagttgtag attataagac
     1441 ttctattgat cagaatatta aatcacagtt tcatcagaat ccatttgatt tctcagcaac
     1501 cttaagatta ggagttggag aatggagtgt ttttggaatg tatgatttga cctccttgta
     1561 caagaaaaat gaagggcctc aactcaaagt ttggaatata ggtgtgacta ttattccatt
     1621 ttgagatgtt atagacaaat ttttggctta taggaaaggg gacccttgaa aaagagtccc
     1681 cttaaatatt ttggtagaat aatctgaaat aagagaaaat gcctatcgct tcttcaattc
     1741 atctcttatt tctgcaagta atgtctcagt tggagatggg ggaggaggcc cggctggagc
     1801 agcaggttcc ggattacgaa gtttattcat agccttgacc atcatgaaaa ttatgaaagc
     1861 caatataatg aaattaatag ctacagtaat aaaattaccc catgcgaaaa ccggcccaag
     1921 ttcctttgct ttatctaaag gtaatccgga tgtaatttta tccgataagg gaatatatag
     1981 attactaaaa tccgcattga acacaatccc aactattggc atgatgagat cattgacaat
     2041 ggaatcgaca atctttccaa atgctccacc aatgatcaca cctactgcaa ggtccattac
     2101 actgcccttg gtagcaaatt ctttaaattc tttaagaaaa cccatagcat atatttttta
     2161 tatattaata atgatacaaa tctaattaaa atattataaa atcatatatg ttatgggcta
     2221 atatgaaaaa attaagtcaa cgacttggtt tttggtatat tcgcttttat ttgataaaaa
     2281 tatttatttt gatgctcttt gcgttattca aataatagcc aaatgaatat ttaacttaaa
     2341 acaaatgtta atgtgttgaa gtgtccttta cttttcctga ctctttttat ttgcaatctc
     2401 aatgttttta gtcaaaatag caggatcgat ggttttgtcg ccgatgaatt gaacggttat
     2461 gcaattcaaa atgcaacagt aacaattatg cgtacttcag attcgctgct ggttaattat
     2521 tcacggacaa aaactgatgg cagtttttca ttcaagtctc ttattcctgg tcattatgat
     2581 ctttttattt cttatccgga ttatttgggt tatgcaacgg atattgaatt agagcccggc
     2641 aaggactttc tgcttgatac ggtctacctc attaaaaata gtcaactact taaggaaatt
     2701 actattgtcg atggtagtaa gatcaagttt aaaggtgaca ccattcaatt tcttgccgat
     2761 agttttagag tgggagaaaa tgccaatgtt gaggatttgt tgaaaaaact ttccggaatc
     2821 aaagtgaatt caaaaggaga aattactgca tttggtgaaa aggttgaaaa ggtatttgtg
     2881 gatggagagg agttctttgg ttctgatccg actattgcaa cccgcaatat tcaagccaag
     2941 gctgttgaca aggtacaggt atttgacaag acttcgacaa tggaagagct taccgggata
     3001 gctgatggag aaaaatttaa agctataaat cttactttaa aagaggaata taaaaaggga
     3061 tacttcggaa aagcaagtgc cggagtagga ttagacccgg tctattatga cggatcatta
     3121 atgttgcagg gttatacagc aaagtctaaa atatcggcat atggtattgg cagtaatacg
     3181 ggcacagtca gccttgattt caatgactat ggtaagtata tgaattcttc tgctggttca
     3241 gtcagttttg atggcaatat gacaaccatc tctttttcaa gtgaggatga ttttagctgg
     3301 gatggtagat atgacggcga gggcctacct aaatcattag cgggaggtgc ttcattttct
     3361 accaaactag ccaaggataa aattcgaatc aatgccaatt atgggtactc tgatatccaa
     3421 ttgaaaaaga atgaaaaaaa tatttcaaca aattatttac cggatgatgg atttgtaaaa
     3481 gatgacagtc aatttacaga tagtaggatt atcaagcatt ctgccttgtt caaagtagaa
     3541 gcagaccttg attctctgac tactttaata tggaacaacg gagcttcgac cagaaaaacc
     3601 aataattata aagaggtaca tacccgacaa aatgatcttg aacagaatcc aataaccgaa
     3661 ataaacagga ctttaactaa taagaatacc ggaaattcaa ttaatagtga attgacatta
     3721 acccgaaaat tcaagaaaaa gcatcgttta ctcgtaatca acggttccgg taactattct
     3781 gaaaatgaag gaagtagcta tcttaattcc aggaatgatt attttacttt gaatcagttc
     3841 cagcagatca atcaattggt tgacaagggt gaaactaatt ttagttatca agcaaaggca
     3901 acatatagtg aacctcttgg aaaaaaatgg actggtcaga tcaatgttta caccagcaat
     3961 gttgctaatc attccaataa attggcaaat gaatttgatg aaataacaca acaatattcg
     4021 aaattcgttg actcgctgag caatgacttt gattatagga ttaattccaa tggagggggt
     4081 gcgtccatca attataaggg agaaaaatac gatttcagat tcggaactga tgttgactac
     4141 tccaatacta ttagaaaaaa caaaattgat tcatcttcta tcattgacaa tgctttaaga
     4201 tggattccgc gagcaaattt tacctataaa tttaatcgcg catcgagaat ttctatgaat
     4261 tactatggtt caacacgcca accttcgatc aatcaaattc aacccgtccg ggacaattca
     4321 gatccgactg ttttaatcaa agggaacccg gctttaaaac aatcctacac ccaatctata
     4381 tcaatgtatt ataatttctg gaaagcgtta tctgacgtag gattgtggtc atatgccaat
     4441 tttagcaata ccttcaatga aattgtgtcc aatacaatca ttgatgaggc aagacggtcc
     4501 atcaccacct ttaccaatgt taatggtgga tataatggaa atgttggatt taat
//
LOCUS       DAOQQN010000271         4517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_8928_length_4517_cov_5.792694, whole genome shotgun sequence.
ACCESSION   DAOQQN010000271 DAOQQN010000000
VERSION     DAOQQN010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8928_length_4517_cov_5.792694"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /gene="sprA"
                     /locus_tag="PLG24_09925"
     CDS             complement(<1..1011)
                     /gene="sprA"
                     /locus_tag="PLG24_09925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HMZ25252.1"
                     /translation="MNLKINTLSLLFVLGIGLGIYAMATGKIIQGGLDVIQPDEKIEA
                     LDTVIPRKPFYPRDTTNPINLKLPPDLESGYNYDPNTNSYFFSNKIGNDYLDAPTNLT
                     FSEYLKEKSKQQERDYFGKLAGLSDGRNSGKAKDPLEGYKTSNSIISRLFGSDVIDIK
                     PSGNVDIDLGLASNHTFNPTATERQRRYTTFVFQPTINLSIAGKVGEKLSVNANYNTQ
                     STLDFDQFIKIKFDGAKGNEDDILKNVEAGNVSLPLRSSLIQGSQSLFGIKADLQFGH
                     LKLTGLISQQKSERKNLQLQGGSQLQTFSIPIDQYDENKHFLLSHYNRSVFNSSLRTM
                     PQI"
     gene            complement(1207..1803)
                     /gene="ruvA"
                     /locus_tag="PLG24_09930"
     CDS             complement(1207..1803)
                     /gene="ruvA"
                     /locus_tag="PLG24_09930"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HMZ25253.1"
                     /translation="MIAYIHGKITGLTPTYALIETSGIGYHVNISLNTYAMLDGKETY
                     KLLTVLLIKEDSHTLYGFATEEERSVFTHLISVNGVGAGTARLILSSMPATDVKQAII
                     REDERMFSMVKGIGPKTAKRIIIDLKDKLSKEGDLPAGNIRLPLVSAIRDEAGAALLA
                     LGFQKNNIDKVLNTLLKEDKEYTLEQMVKAALKSLSGA"
     gene            complement(2006..3388)
                     /locus_tag="PLG24_09935"
     CDS             complement(2006..3388)
                     /locus_tag="PLG24_09935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HMZ25254.1"
                     /translation="MGVKGRLTIISFMQFFVWGAWLITMANFWFGTKNWDGTQFGAVF
                     GTMGIASIFMPTLVGIIADRWINAERLYALLHILYGAVLFYLPNTTDSGAFFYTMLVA
                     MCFYMPTIALCNSISYNILKSNNYDVIKEFPPIRVWGTIGFIVAMWTTNLTGQKATEG
                     QFYIAGVAAILLGLYALTLPKCPPQKLIDKDAPLTHQLGLNAFRLFADYKMALFFIFS
                     MMLGAALQLTNAYGDVFLDEFKYFPKYASSFVIKYSTIIMSISQISETLFILAIPFFF
                     KRFGIKQVMLISMLAWVFRFGLFGFGDPAGGLWMIILSCIVYGMAFDFFNISGSLFVE
                     TTTEPKIRSSVQGLFMMMTNGFGAVLGSVIAGWSIDRFFTKKFIDIQSLAANFDTTAD
                     NPTFLDIAKNALGAVINQDGSLANPVYIKDWHQIWIAFAAYALLVTILFALLFKNEPK
                     QELDLSKLNH"
     gene            complement(3563..4066)
                     /locus_tag="PLG24_09940"
     CDS             complement(3563..4066)
                     /locus_tag="PLG24_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25255.1"
                     /translation="MLTKLTFIPLIIMLILSMNSGLAMIHSPDSTGPKVFLLGDDENL
                     MNSLSKNYPTLLMKVCKDDMEVAYNVWMEMLSDMEAYATKINFDIRGTKLWMNVYWNK
                     NGKIEYIGYYLKPNSRNVKTEEMNAFFASFMKNYQMNLSYTGRFYHNGSAAFPTHFKA
                     LKDKEGG"
     gene            complement(4242..>4517)
                     /locus_tag="PLG24_09945"
     CDS             complement(4242..>4517)
                     /locus_tag="PLG24_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HMZ25256.1"
                     /translation="EEVRAGSLEPGIIDNRIIEQHLYTSGMPQPELLIRTSGEYRISN
                     FLLWQIAYTEFYFTPLMWPEFGQQEFYKAIIEFQSRERRFGKISEQL"
BASE COUNT         1301 a          881 c          941 g         1394 t
ORIGIN      
        1 gatctgaggc atggtcctca aggatgaatt gaacaccgac ctgttgtaat gagataaaag
       61 gaagtgtttg ttttcgtcgt attggtcaat agggatggag aatgtctgca attggcttcc
      121 accttgaagt tgtaggtttt tacgctctga tttttgttga gaaattaatc ctgtcaactt
      181 gagatgccca aattgcaggt cagccttgat accgaatagc gattggctac cttgaatcag
      241 cgagcttcgc agcggcaggg atacattacc ggcttctaca ttcttgagaa tatcgtcttc
      301 gttgcccttt gcgccatcaa attttatttt gataaactgg tcaaagtcca aagtagactg
      361 tgtattatag ttggcgttga cactaagctt ttctcctact tttccggcta tgctaagatt
      421 gatagtaggt tggaatacaa aagtagtata tcttctttgg cgctcagtcg cagtagggtt
      481 aaaggtgtga ttggaagcca gaccaaggtc gatgtccacg tttccggagg gcttgatatc
      541 tattacatca ctgccaaaaa ggcgcgaaat aatggagttg ctggttttgt aaccttccag
      601 tgggtctttg gctttgccgg agttcctgcc atcggacaat ccggctaatt tgccgaaata
      661 gtccctttcc tgttgctttg atttttcttt gaggtattca ctgaaggtta gattggtggg
      721 ggcatccaga taatcattac ctattttatt ggagaaaaaa tagctattgg tatttggatc
      781 gtaattgtag ccggattcaa gatcaggagg gagtttgagg ttgattggat tggtggtgtc
      841 gcgcgggtag aatggcttac ggggaatgac agtatcaagg gcttcgattt tttcatccgg
      901 ttgaatgacg tcgaggcctc cctgaattat tttgcctgtc gccatagcat atattcccaa
      961 tccgataccg aggacgaata atagacttaa agtgttgatt tttaaattca tataaattta
     1021 tcaacgcgtt atatcaacgg ctttttattt taattttatc cgaaccgaca agatccggca
     1081 ttattaatgg aatttttaat tgttcggggc gaatgttaat tattcataat tgtggtcata
     1141 taaatatatg ttttattcaa aacgaaaaag ttcctttttt cttttattct taataaaatt
     1201 aacattttat gcacctgata agctcttcaa tgcagccttt accatctgct ccaaggtata
     1261 ttctttgtct tctttcagta atgtattgag taccttgtcg atgttatttt tttgaaaacc
     1321 aagtgccaga agtgccgcac cggcttcatc ccggatggcg gataccagag gcaatcggat
     1381 atttccggcc ggaaggtcac cttctttgct tagcttgtct ttaagatcta tgatgattct
     1441 tttagccgtt tttgggccaa tccccttgac cattgagaac atgcgttcat cctctcgtat
     1501 gattgcctgc ttgacatcgg ttgcaggcat agaggaaagt ataagtctgg cagtacctgc
     1561 acccacacca ttgacagaaa tcaaatgcgt gaaaacactt cgttcttctt cggttgcaaa
     1621 gccatacaaa gtatgagaat cctctttgat cagcagaaca gtaagcaact tataagtctc
     1681 cttcccatcc agcatcgcat aggtattgag gcttatattg acgtgatatc ctatgcccga
     1741 tgtctctatc aatgcataag tcggtgtcag tcccgtaatt ttgccatgga tatatgcgat
     1801 cattgcaatt cgccctaatt ttcagcgtgc aaaggtaatc aaattataca aaaagtaaat
     1861 ttattaaaat caatggtaca atatattctt caggatttgt tgactaaagc tcttcttgtc
     1921 atgaagtata gttgacaata tccttttctt aacatacgcg gttactggta ctccaactgt
     1981 ctgaatacag gtatttactt ttgtatcaat ggttcaattt actcagatca agctcttgtt
     2041 ttggctcatt tttgaaaagg agagcaaaaa gtatggtgac aaggagtgca tacgcggcaa
     2101 atgcaatcca tatctgatgc cagtccttaa tatatacggg attagcaagg ctgccatctt
     2161 gattgatcac tgcccccaaa gcattttttg caatatcgag aaaggttgga ttgtccgctg
     2221 ttgtatcaaa gttggctgca agagattgaa tatcaatgaa ttttttggtg aagaaccgat
     2281 caatggacca gccggcaatg acactaccca acacagctcc gaaaccatta gtcatcatca
     2341 taaacaagcc ctgtacagaa gaacgaatct taggttcagt cgttgtttct acaaataaag
     2401 atcctgaaat attaaaaaag tcgaaggcca taccatagac aatgcatgat aggatgatca
     2461 tccaaagccc acctgcagga tcgccaaatc caaataaacc gaacctgaac acccatgcca
     2521 gcatgctgat gagcattacc tgtttgatgc cgaatcgctt gaagaaaaac gggatggcaa
     2581 ggatgaaaag tgtctcggat atctgtgata tggacatgat aattgtcgaa tacttgatta
     2641 caaacgaaga tgcatatttg gggaaatatt tgaattcgtc cagaaatacg tctccgtagg
     2701 cattggttag ttgtaatgct gcgccaagca tcatcgagaa tatgaagaaa agtgccatct
     2761 tataatcagc aaacaacctg aaggcgttta atcccagttg atgagtcaaa ggagcatctt
     2821 tgtcgatgag tttctgaggt gggcatttag gtaaggtgag agcatataaa cccaacaaaa
     2881 tggccgccac tcctgctata tagaactggc cctctgtcgc tttctgtccg gtgaggttgg
     2941 tcgtccacat agcgacaata aacccaattg tcccccagac cctgataggc gggaattcct
     3001 tgattacatc ataattgttg gatttcagta tgttgtaaga aatggaattg cacaaagcga
     3061 tggtcggcat ataaaaacac atcgcaacta acatggtata gaaaaaggcg cccgaatcgg
     3121 ttgtgttggg aagataaaac aatacggcac cataaaggat gtgaagcagc gcataaaggc
     3181 gctcggcatt gatccatcgg tctgctatga tgccgaccag tgtgggcata aatattgatg
     3241 caatgcccat agtgccaaac actgccccaa attgcgttcc atcccagttt ttagtgccaa
     3301 accagaaatt ggccatggtg ataagccatg ccccccaaac aaaaaactgc ataaaactga
     3361 ttatcgtcaa tctgccttta actcccacgt tattgatatt ttaatatttg gttacaaata
     3421 aaaataaaat atccaacttt tttgcaaaaa caggaattaa cacagatttt ttatcggcat
     3481 tttcaaccga tggcaatacg atgcttcaaa acaagatgtg aaattaataa ggttggggat
     3541 tgatcatgcg aaaagggtct tgtcatccac cttctttatc cttgagtgcc ttgaaatgag
     3601 tcggaaatgc tgcacttcca ttgtggtaaa aacggccggt ataagatagg ttcatctgat
     3661 aatttttcat aaagctcgca aaaaaagcgt tcatttcctc agtttttaca tttctgctgt
     3721 tcggctttag atagtacccg atatattcaa ttttgccgtt tttattccaa tagacgttca
     3781 tccacagctt ggtgcctctg atatcaaaat tgattttagt ggcatatgct tccatatctg
     3841 agagcatttc catccataca ttgtaggcta cctccatatc gtccttacac actttcatga
     3901 gtaaagtggg atagttttta ctgagagaat tcatcaaatt ttcatcatca cccaatagaa
     3961 aaaccttcgg gccggtagaa tccggtgagt gtatcatggc taatccagag ttcattgaca
     4021 ggatgagcat gataataagt ggtatgaatg ttaatttagt caacaatttt ttcaagtaat
     4081 ttggcaaaga taataaaacc acgataataa aatattttca cgaataagat gtttgttttt
     4141 tatccgatgg tctagattaa ttttgaattt tttgattata aaacgttttt attgtgttct
     4201 attacacaga atcaggctta ttgttttgtt aaatttatcg atcagagctg ttcggatatt
     4261 ttgccaaacc gccgttctcg tgactgaaac tcgatgatgg ctttgtaaaa ttcttgctgt
     4321 ccaaattccg gccacatcaa aggagtgaag tagaattctg tataagctat ctgccaaagc
     4381 aggaaatttg atatccgata ttctccgctg gttctgataa gtaattctgg ctgaggcatg
     4441 ccggatgtgt acaggtgttg ttcgataatc cggttgtcga tgatgccggg ttccagggaa
     4501 ccggccctaa cctcctc
//
LOCUS       DAOQQN010000272         4488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9005_length_4488_cov_3.619896, whole genome shotgun sequence.
ACCESSION   DAOQQN010000272 DAOQQN010000000
VERSION     DAOQQN010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9005_length_4488_cov_3.619896"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLG24_09950"
     CDS             complement(<1..181)
                     /locus_tag="PLG24_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HMZ25257.1"
                     /translation="MIQIKRIYAKNEATDGYRVLVDRVWPRGVSKESAHIDEWLKELG
                     PSTELRKWFGHIPQKF"
     gene            complement(312..1034)
                     /locus_tag="PLG24_09955"
     CDS             complement(312..1034)
                     /locus_tag="PLG24_09955"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HMZ25258.1"
                     /translation="MSPDIIFEDDHLIIVSKPSGILSIPDRHDPSKANMQDILSRKYG
                     KIFTVHRIDRETSGIMCFAKTAEVHAALSNMFQQREIEKTYLAFVSGNVQPEEGSIEA
                     ALMPDPLHSGRMKVSPTGKKSLTTYKVIERFKNCCLVEAGILTGRMHQIRVHFKYIGH
                     PCLVDKLYGTNEAIFIRDIKLKNLKVVKNIDTDERPLVARTTLHAFRLKLVHPATEKE
                     MIFEAPFPKDLKALLNQLRKWA"
     gene            complement(1024..3000)
                     /locus_tag="PLG24_09960"
     CDS             complement(1024..3000)
                     /locus_tag="PLG24_09960"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HMZ25259.1"
                     /translation="MEVYYNLESFPRLENAVVTIGSFDGVHLGHMTLIDQVISLAKYA
                     GGPSVVVTFDPHPRLILEINPENFGLLTSLDEKIKLIADCGVDYLFVVPFDTAFSKQS
                     AKAYLHDFLVHHVNPRTIVVGYDHRYGAGRKGDFELMEKELSPLGIRIVEIDPLLIDR
                     IAVSSSSIRRALASGNIEKVNSDLGYEYTITGKVTHGDKIGRTLGFPTANLEITEQHK
                     ALPKDGIYLTKVIIEDKIHHGLAYIGHRPSINSDLLRAVEVYIYDFDSDIYGSQVTLK
                     LIDFIREDRKIDGLDALKAQMEEDKEAGRRLITGITPSSKNLAVVVLNYNGAVHLRKY
                     MPDLIKYSPQADIVLADNCSTDDSLDYLKTLEKDIKIIMLDQNYGFTGGYNRAIASIS
                     HPYVLLLNSDVRVTEDWLIPLLSLIEEDPSIGAIQPKMLNDREPALFDYAGASGGWMD
                     RLGYPFCRGRIFGDIESDGKQYDDRAEIFWATGAAMLTRTELYKKLGGLDEYFFAHME
                     EIDLCWRMKNAGYKIMVEPASKVYHYGGGTLDASSPRKTYLNFRNSLFVLLKNEQHKV
                     WSKIFARLCLDGIAGIRFFLQGKFSFVSAILKAHFNFYANIGRYMKARKLITVQPLNS
                     VRPLTGKYDGLIISQYFIKGKKKFSDIIIENNES"
     gene            3290..4384
                     /locus_tag="PLG24_09965"
     CDS             3290..4384
                     /locus_tag="PLG24_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HMZ25260.1"
                     /translation="MNIDISEIKQLLTTIRDPRTGTNIVQAGIVKDVLLQGNDLAIQI
                     YSSNMDSTDKGNLTQAIVFSIQATYPELEVNIQMVNRAPDIQDRPSPLPQVKNIIAVG
                     SGKGGVGKSTISVGLAFALNRLGYKVGLLDADIYGPSIPTMLGLKGKRPPITNVYGKT
                     KLMPIDVNGVQTMSIGNIIEPDQAVVLRGPRLTGIIKQFIEDTVWEELDFMIIDLPPG
                     TGDIQLTLVQTVPLSGAVIVTTPQEVAFADALRAMNMFRMDQINVPIIGVIENMAWFT
                     PRELPDNKYFIFGEGGGERLAKLSHSSILGQVPLIMGLRESGDQGTAYKTDKEGHNSK
                     IFEEVGKNLILQLNKVLALQGRAKIVEIKS"
BASE COUNT         1241 a          966 c          952 g         1329 t
ORIGIN      
        1 gaaatttttg cggaatgtgc ccaaaccatt ttctcaattc agtcgaaggc cccaattctt
       61 tcaaccattc atcgatatgg gcgctttctt tgcttacacc tctcggccag acacgatcga
      121 ccagtactcg atatccatca gttgcttcgt ttttcgcata tatacgtttg atttgaatca
      181 ttgctacctc cttttaatac aatcatgttg catttaaaaa ttaaaacttg taatgaggca
      241 aatggttcat tggaccggga gcataaatta tcctttctcc ggattttatt actgtgtaat
      301 gaaatccaga gttatgccca ttttctcagt tggtttaaca gtgccttcag atctttaggg
      361 aatggtgctt cgaaaatcat ttctttttcg gtggccggat ggacaagttt caacctgaaa
      421 gcgtggaggg ttgtcctcgc aaccaggggc ctttcgtcgg tgtctatatt ttttactacc
      481 ttaaggttct tgagcttgat atctcggata aatatagctt cgttagtacc ataaagtttg
      541 tcgaccagac acggatggcc tatgtacttg aaatgtaccc ggatctgatg catccgtccg
      601 gttaagatac ctgcctccac taagcaacag tttttaaatc tttctatgac cttataagtt
      661 gtaagtgatt tttttccggt agggctgact ttcatacgac cggaatggag cgggtcaggc
      721 atcaacgcgg cttcaatact cccttcttca ggttgaacgt tacctgagac aaaggccagg
      781 taggtttttt caatttctct ttgctgaaac atattactga gtgcagcatg tacttctgcg
      841 gttttagcaa agcacatgat gcctgatgtt tccctgtcaa tgcggtgcac ggtgaatatt
      901 tttccatact ttcgtgacag tatgtcctgc atgttggctt ttgagggatc atgcctgtcc
      961 ggaatagaca atatacccga tggtttggag actattatca aatggtcgtc ttcgaaaatg
     1021 atgtcaggac tcattgtttt ctattatgat gtctgaaaat ttcttttttc ctttgataaa
     1081 atattgactg atgatcaggc catcatactt gccggtcaat ggtcttactg aattcaatgg
     1141 ctgaacggta atgagcttcc ttgccttcat ataccgacct atatttgcat agaaattaaa
     1201 atgtgcctta agaatggcac ttacgaagga aaatttgccc tgtaggaaaa aacgaatacc
     1261 tgcgatacca tccaaacaaa gtcgggcgaa tatcttactc cagactttgt gctgttcatt
     1321 ctttaacaat acaaacagac tgttgcggaa gttaagatag gtctttctcg gtgaggatgc
     1381 gtccaatgtg ccacctccat aatgatagac tttggaggcc ggttcaacca tgattttata
     1441 acctgcgttt ttcatacgcc agcacaagtc gatctcttcc atatgagcaa aaaaatattc
     1501 atccagtccg cctaatttct tatataactc cgtacgtgtt agcatagctg caccggtggc
     1561 ccagaatatt tctgctctgt catcatattg cttcccgtca gactcgatgt ctccgaagat
     1621 ccgacctctg caaaacggat atcccagtct gtccatccaa ccccctgatg ccccggcata
     1681 gtcaaataac gcaggctctc tgtcatttaa catcttgggt tgtatcgcgc ctatggaagg
     1741 gtcctcttct atcaggctta gtaaagggat gagccaatct tcagtgaccc gtacatcaga
     1801 attcaataag agtacataag gatgggaaat agaagctatt gcccggttgt atccacctgt
     1861 aaatccatag ttttggtcca gcattatgat ctttatgtcc ttttccaaag tcttaaggta
     1921 atctaaagaa tcatcagtgg aacaattgtc ggccaagaca atatctgcct ggggcgaata
     1981 tttgattaga tcaggcatgt attttctgag gtggactgca ccattgtagt ttaatactac
     2041 aacagccaga ttcttactgg atggagtgat tcctgttatc aaccgtcttc cggcttcctt
     2101 atcctcttcc atctgtgctt ttagcgcatc caggccatca atttttctat cttcccgtat
     2161 aaaatcgatc aacttcaatg ttacttgtga gccgtaaata tcagaatcga aatcatagat
     2221 atagacctct acggcacgga gcaaatcgct gttgatggat ggtcgatggc cgatatatgc
     2281 cagtccgtga tgtatcttat cttcgataat gacctttgtt agatagatac catctttagg
     2341 aagtgcctta tgctgctcag tgatttcaag gttggctgta ggaaaaccta gtgttctccc
     2401 aattttgtcg ccatgagtga ctttgcccgt tatggtatac tcgtaaccta agtcactgtt
     2461 tactttttca atattaccac tggcaagggc tctcctgatt gaagacgaac tgactgcgat
     2521 gcggtcgatc agcagggggt ctatttccac aattcgaatt ccgagagggc tcagttcctt
     2581 ttccatcagc tcgaaatcgc cttttctgcc cgctccgtaa cgatgatcgt aaccgaccac
     2641 aatggtgcgg ggattgacat gatgtaccag aaagtcatgg agatatgcct ttgccgattg
     2701 tttggagaac gcagtatcaa atggaacaac aaaaagataa tcaaccccac aatcagcaat
     2761 gagtttgatt ttttcatcca aacttgtgag gaggccgaag ttttcggggt ttatctccag
     2821 gataagtctt gggtggggat caaaggttac aactacggaa gggccgccgg catattttgc
     2881 aagtgatatt acctgatcaa tcaaggtcat gtgcccgaga tgcaccccat cgaaagaacc
     2941 gatggtaaca acggcatttt ccagccgggg aaatgattca agattgtagt aaacctccat
     3001 aaaattagct gcaaatgtac aaagccggta gtgtaaaatg aattatcttt aaatatttgt
     3061 tgaaaaacct cattttggtg aagcctgtat tatttacctt ttttttattg tttaggtgaa
     3121 ttcaatgaaa gcatcactca ccggaaatgt caggaaatac atgcccacgg tattgttcaa
     3181 atttccatat attaagacgt aaatatcttt tatatatttg agctcagaaa ctattggtaa
     3241 aatttgccgg gtggatacaa ccttttaata accttaatgt taaaaagcca tgaatattga
     3301 cattagcgaa ataaaacaac tactgactac cattcgagac cccaggaccg gtaccaatat
     3361 cgtacaggct ggtatcgtca aagatgtatt gctccaggga aatgaccttg ctattcagat
     3421 ctattccagt aatatggaca gtactgacaa agggaatctc acacaggcta tcgttttcag
     3481 tatacaggct acttaccccg aactcgaagt taatatccag atggtcaatc gcgcccctga
     3541 tattcaggac agaccttcac cactgccaca ggtcaaaaat atcatagcag taggttccgg
     3601 taaaggaggg gtaggcaagt ctaccatttc tgtcggcctg gcatttgctc tgaatagact
     3661 gggctacaag gttggccttt tagatgcaga tatctacgga ccatctatcc caaccatgtt
     3721 gggtttgaaa gggaaacgtc ctcctattac caacgtatat ggcaaaacca aactcatgcc
     3781 tatcgatgtt aatggcgtcc agactatgtc gattggcaat atcatcgagc cggatcaagc
     3841 cgtagtcctg cgcgggccaa gactgaccgg aatcatcaaa cagtttatcg aggataccgt
     3901 ttgggaagaa ctggacttta tgatcatcga tctgcctccg ggtaccggtg atatccaatt
     3961 gacccttgta cagaccgtgc cattatccgg tgctgttatc gtgactacgc cccaggaagt
     4021 ggcatttgcc gatgcactca gagcaatgaa tatgttccgt atggaccaga tcaatgtccc
     4081 catcatcggt gtcatagaaa atatggcttg gtttacaccc agagaattgc cggataataa
     4141 gtattttatc tttggtgagg gaggaggtga gaggcttgct aaattaagcc attcatccat
     4201 tttgggtcaa gttccgctta tcatgggact gcgggaatcc ggcgatcagg gaactgccta
     4261 taaaaccgat aaagaaggtc ataattcgaa gatctttgag gaagtcggaa aaaaccttat
     4321 cttgcagctc aataaggtac ttgcattgca aggcagggca aaaatagtcg aaatcaaatc
     4381 ctgataggaa tgaatgaact tttggaacga attgaatctg cactggatga ggtaagacca
     4441 catctcagag tagatggtgg agatatagaa gtgcttgaaa taacacct
//
LOCUS       DAOQQN010000273         4484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9016_length_4484_cov_5.306841, whole genome shotgun sequence.
ACCESSION   DAOQQN010000273 DAOQQN010000000
VERSION     DAOQQN010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9016_length_4484_cov_5.306841"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /gene="tpiA"
                     /locus_tag="PLG24_09970"
     CDS             <1..578
                     /gene="tpiA"
                     /locus_tag="PLG24_09970"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HMZ25261.1"
                     /translation="NISRHDKGAFTGEVSAGMLHSVGCQYVLIGHSECREHFHENGES
                     LVGKILRAFEHNLTPIFCCGESLKERESNSYADFVANQIREALFELSEEQLGRLVIAY
                     EPIWAIGTGHTARPDQAQEIHAIIREILKERFPETLVENLSILYGGSVNAVNACELFS
                     QPDVDGGLVGGASLKADEFVSIINAMEDRFN"
     gene            595..1008
                     /locus_tag="PLG24_09975"
     CDS             595..1008
                     /locus_tag="PLG24_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HMZ25262.1"
                     /translation="MEHWLIKSEPEEFSFEDLEREGTTEWTGVRNYAARINLNNMRNG
                     DICLFYHSVTDKRIVGLATIVEEAYPDPTDETGKWVAVKVAYLKPLNKPIEYKVLKEM
                     DHMKKFDLIRISRLSVMPVPDEFWYEILEMSETTV"
     gene            1012..1401
                     /locus_tag="PLG24_09980"
     CDS             1012..1401
                     /locus_tag="PLG24_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25263.1"
                     /translation="MKNLLLLILGVSLFTSCKKTIISPPVTYKGIWQAGSFVVKDSSV
                     TLDVSNIILKLNEDSRYSFVNNIGQNEAGVYVVRDSLLVVTDTTVSPSVEKAMQITGL
                     TSDSLVLRMNFGGKESWMHFTKQKESK"
     gene            complement(1495..3120)
                     /gene="pruA"
                     /locus_tag="PLG24_09985"
     CDS             complement(1495..3120)
                     /gene="pruA"
                     /locus_tag="PLG24_09985"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HMZ25264.1"
                     /translation="MSNAIFKTPRPVNEVVRSYAPGTEDRKQLEAAIKEAKSKTHDIP
                     MYIGGKEVRTDNKVAIRPPHEIKHTLAYYHKGDENHVKAAIDAALGAKAQWSSMPWEH
                     RAAIFLKAAELIAGPYRMKINAATMLGQSKNAFQAEIDSACELIDFLRFNVEYMTEIY
                     KIQPESSPGIWNRLEWRPLEGFVFALTPFNFTAIAGNLPSSPALMGNTVVWKPSETQI
                     FAANVIMEVFREAGLPDGVINLVFVSGAVAGDVIFSHRDFAGLHFTGGTSTFNTIYRK
                     IASHLDDYRSYPRIVGETGGKDFVMVHKSANPDEVAVALARGAFEYQGQKCSAASRAY
                     IPRSLWPAIKERLQKDVESMKMGSTEDFSNFINAVIDKKAFDFITSYIDRAKQSDVAK
                     VIIGGEYDDSEGYFIRPTVIETTNPKYDTLCTELFGPVLTVYVYEDDQFQETMKLLDE
                     TSDYALTGSIFAKDRYAIDLAVKALENSAGNFYINDKPTGAVVGQQPFGGARGSGTND
                     KAGSVLNLYRWVSARTIKETFVPPTDYRYPFLG"
     gene            complement(3219..3674)
                     /gene="coaD"
                     /locus_tag="PLG24_09990"
     CDS             complement(3219..3674)
                     /gene="coaD"
                     /locus_tag="PLG24_09990"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HMZ25265.1"
                     /translation="MKIAVFPGSFDPITVGHFDLIVRSLALFDKVIVAIGINDAKSYL
                     YPLEKRMEWLRIVFAEYDKVEVDHYRGLTADFCKKVKANFLVRGLRNSSDFDYEKTIS
                     QVNYIITGGIETIFLISAPKYSHISSTIVREVIKGGGDVKPFLPKGVVL"
     gene            3931..>4484
                     /locus_tag="PLG24_09995"
     CDS             3931..>4484
                     /locus_tag="PLG24_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25266.1"
                     /translation="MRSLIWLLCFLVIGHDVADAQSVPWYALNGSDVRAARSLNPAFL
                     QNPHFESALGIGSISMDYNNNLGYFIDKSFMSLPIHFSEISVPELQFRKLGFNPALGI
                     DRELRGYEMKLNETDLARMYFSTSLSIAGPSYFRRLKSGMIVGITTGSSFIGGLKNYP
                     AHMTYGPYRRYSSGDRMPVPEFSGT"
BASE COUNT         1270 a          892 c          980 g         1342 t
ORIGIN      
        1 agaatatcag ccggcacgat aaaggggctt ttaccgggga agtatctgcg ggtatgctcc
       61 attcagtcgg ttgccagtat gttttgatcg gtcactcaga atgcagagaa cattttcatg
      121 aaaatggtga aagcctcgtt ggtaagatac ttcgggcttt tgaacataac ctaacaccga
      181 tcttttgttg tggcgaatcc ttgaaagaaa gggaatcgaa ctcatacgcc gactttgtcg
      241 caaatcagat cagagaagcc ttgtttgaat tgtctgaaga acaattggga aggttagtca
      301 ttgcttatga gccgatatgg gccattggga caggtcatac agccagaccg gaccaggctc
      361 aagaaataca tgcgattatc agggagattt tgaaggagcg atttcctgaa acattggtgg
      421 aaaatctgtc aattctatac ggaggaagtg ttaatgctgt taatgcttgt gaattattct
      481 cacaaccgga tgtggatggc ggactggtgg gaggtgcatc attgaaggct gatgagttcg
      541 tttctattat caatgcaatg gaggataggt ttaattaaat taataaattg gaatatggaa
      601 cattggttga ttaaatccga gccggaggaa tttagttttg aagatcttga gagggaaggg
      661 acgactgagt ggaccggtgt acgcaattat gctgccagaa tcaatctgaa taatatgaga
      721 aatggcgata tctgcctttt ttatcacagt gtcaccgata aaagaatcgt tggcctggct
      781 acaattgttg aagaagctta tcctgatcct accgatgaaa ccggaaaatg ggtagcagtt
      841 aaggttgctt acctcaaacc gctcaataaa ccgattgagt ataaggtgtt gaaagagatg
      901 gatcacatga aaaagttcga tctgatcaga atatccaggc tttccgtaat gcctgttccc
      961 gacgaattct ggtacgaaat ccttgaaatg tcagaaacta ccgtttgatc gatgaagaat
     1021 ctgttacttt taattttggg cgtctctttg ttcacctctt gcaaaaaaac tattatatcc
     1081 ccccctgtta cttacaaggg aatttggcaa gcaggatctt tcgtagtgaa ggatagttct
     1141 gttaccttgg atgtcagcaa tatcattcta aaactgaatg aagattcccg gtacagtttt
     1201 gtaaataata ttggtcagaa tgaagcaggt gtctatgtag taagggatag tcttctggtt
     1261 gtcaccgaca caacggtatc tccttcagtt gaaaaagcca tgcagatcac cggtttgact
     1321 tcagacagcc tggtattgcg tatgaatttt ggaggaaaag aatcctggat gcatttcacc
     1381 aaacaaaaag aatcaaagta aacctaaccc tgcattttat tattttcata gaataggaac
     1441 aaaaatggga tgccataagc agcccatttt tttatcaaat aatcttgttt tcaattatcc
     1501 aaggaaggga tatctgtaat cggttggcgg aacgaatgtt tccttgatgg tcctggctga
     1561 tacccatctg taaaggttaa gtacagaacc ggctttgtca ttggtacctg accctctggc
     1621 ccctccaaat ggctgttgac ctacaacagc gcccgttggc ttatcattga tgtagaagtt
     1681 gccggcactg ttttcaagtg cttttactgc cagatcaatc gcatacctgt cttttgcaaa
     1741 gattgagccc gtcagtgcat aatcagaagt ttcgtccagt aatttcatgg tctcttggaa
     1801 ttgatcatct tcatatacat ataccgttaa taccggcccg aataactcag tacaaagggt
     1861 atcatattta ggattggtag tttcgatgac agtcggtctg atgaagtatc cttctgaatc
     1921 gtcatactca ccgccgataa tgacctttgc tacatcagat tgtttagctc tatctatata
     1981 agatgtgata aagtcaaagg ctttcttgtc gattacggcg ttgatgaaat tggagaaatc
     2041 ttcagttgac cccattttca tagactctac atccttttgg agcctttctt tgatagcagg
     2101 ccataaggaa cggggaatat atgccctgga tgctgctgag catttctggc cctgatattc
     2161 gaatgcgcca cgtgcaagtg ccactgcaac ctcatcaggg ttagcacttt tgtgtaccat
     2221 tacaaagtcc ttgcctcctg tctcacctac tattcttgga taagaacggt aatcgtcaag
     2281 atgacttgca atttttctgt atattgtgtt gaaagtcgat gtgcccccgg taaagtgcaa
     2341 cccggcaaaa tccctgtggc tgaatattac atcacctgcc accgctccgg aaacaaagac
     2401 aaggttgatc acaccatctg gcaatccggc ttccctgaat acttccataa tgacattggc
     2461 cgcaaatatt tgggtttcag atggcttcca tactaccgta ttgcccatta gtgcaggaga
     2521 gcttggcaga ttcccggcta tcgcagtaaa gttgaaagga gtcagggcaa atacaaaacc
     2581 ctctaatggc ctccattcca gtctgttcca gatcccgggc gatgattccg gctggatttt
     2641 atatatttct gtcatatact cgacattgaa tcgcaggaag tcaattaatt cacaggcact
     2701 gtcaatctca gcctgaaagg cattcttgga ctgtcccagc atagttgcag cattgatctt
     2761 catacggtaa gggccggcaa tcaattcggc tgccttaagg aagatggctg ctcggtgttc
     2821 ccagggcata ctgctccatt gggcttttgc tccaagggca gcatctattg cagccttgac
     2881 atgattttca tcccctttat ggtaataggc aagcgtatgc tttatttcat gtggtgggcg
     2941 tatcgcaact ttattatctg tcctgacttc ttttccacca atatacattg ggatatcatg
     3001 ggtcttactc ttggcttcct tgatggcagc ctccagttgc ttcctgtctt cagtaccggg
     3061 agcgtatgaa cggacgacct cattgaccgg ccgtggagtt ttaaatattg cgtttgacat
     3121 aaaaatggtt tataatttat gataattaat taaggtcgtg tgaggcaatt gatacatatg
     3181 aacgagaaga accgaacaaa aggaataatc gtcatttctt ataacacaac tcctttgggc
     3241 aaaaatggtt taacatcccc gccaccttta atcacctctc gcacgatcgt ggaactaata
     3301 tgagaatact tgggcgcact aatgaggaag atggtttcga ttcctcctgt gatgatgtaa
     3361 ttgacctgag agatggtctt ttcataatca aagtctgatg aatttcgaag tcccctgact
     3421 aaaaagttag ctttgacttt tttacagaaa tctgctgtca gcccccgata atggtcaacc
     3481 tcaaccttat catattcagc aaagactata cgtagccatt ccattctttt ttcaagcgga
     3541 tacaaatatg acttggcatc attgatgcca atagcgacaa taaccttgtc aaaaagtgcc
     3601 agactgcgga caatcaagtc aaaatgtcca acggtaatcg gatcgaaaga gcccggaaat
     3661 actgctattt tcatcaaacg ttactttcag gataatggtt attcaacatc atttgatatc
     3721 gaaaaatatg ttgcgttttt tgctaaataa tgaatagatc gggaaagata gcgattcgtc
     3781 atcagtcaaa caaatatcat attatatgat attgaactgt atgaatatta gtggataatg
     3841 cattattgta aaaatatatg tggttattaa caaaaagttg ttaaactatt gattattttt
     3901 gttaactatt tttatattag gctacttctt atgagatcat tgatatggtt gttatgcttc
     3961 ctggtaatcg ggcatgatgt tgcagatgcg cagtcagttc catggtatgc gttgaacgga
     4021 agcgacgtga gggctgccag atccctcaat ccggcatttt tacagaatcc ccatttcgag
     4081 tctgcattag ggattgggtc gatctctatg gactacaaca ataatcttgg atatttcatt
     4141 gataaaagct tcatgagtct tccgatacat tttagtgaaa tatctgtgcc ggaattacag
     4201 ttcagaaaac ttggatttaa tccggctctt ggtatcgaca gagaactacg cggttatgaa
     4261 atgaaactga atgagactga tttggccaga atgtactttt cgacctcttt gtcgatcgca
     4321 ggcccttctt atttcaggag attgaaaagc gggatgatcg taggtataac aacggggtct
     4381 tcattcatcg gaggattaaa gaattacccg gcacatatga cgtatggtcc atatagaaga
     4441 tattcctccg gagatcggat gcctgtacca gagttttcag gtac
//
LOCUS       DAOQQN010000274         4482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9026_length_4482_cov_6.537158, whole genome shotgun sequence.
ACCESSION   DAOQQN010000274 DAOQQN010000000
VERSION     DAOQQN010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9026_length_4482_cov_6.537158"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PLG24_10000"
     CDS             <1..476
                     /locus_tag="PLG24_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HMZ25267.1"
                     /translation="ESYNYAGTSVNWNHTAEPGVSHTMNYVDGFTGDPGTVDILYPVN
                     NVYKGGAVRNDRLTKIITQKYLAQVPWLPLEAWNDKRRLGLPFFENPAVEDVLPNLPD
                     LNSSNFMTSSIKFYAQRLKYPSSLSNSSKDGYNQAVSQLGGPDNVLTPLWWAKQN"
     gene            complement(941..1111)
                     /locus_tag="PLG24_10005"
     CDS             complement(941..1111)
                     /locus_tag="PLG24_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25268.1"
                     /translation="MSNIFKIKGAQRLTMGEVRKSYNISVPLTSILISKIENSECIFL
                     SGKSVLNSKMEA"
     gene            1329..3761
                     /locus_tag="PLG24_10010"
     CDS             1329..3761
                     /locus_tag="PLG24_10010"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HMZ25269.1"
                     /translation="MKRILIPVFLIIITSGLLSSQTNQSEIIFDSPATHFTRSLPLGN
                     GRLGAMIFGDPHHERIVLNEISLWSGGPQNADRDSARYFLPAIRDFLLNGDNASAQEL
                     LRREFVAKGAGSGFGNGANVPFGCYQTAGELRVDYNNIGTVTDYHRVLDLEVAKSKVS
                     FRVNDTRIIQEAFCDFSDDVIRIRWHSSAPAGLNFSLSLFRKENVSSVNYKHGVLSLE
                     GTLPNGENKGMSYGIRLKVIGCDGNFNFSNGKLNISNAGTCSVIIAIRTNYNYAAGGL
                     LPSTIVSKSLKSDLKASTCHDFHRAWIKSSAIYQKYFNACRLQLFPDENQKVNSLTTP
                     QRLEQYANGGSDVGLPVLYFNFGRYLMICSSKPGLLPTNLQGLWAEEYQTPWNGDYHL
                     NINLQMNYWLAETTNLPAQLKPLFQWTSRLVPNGRKTATAYYGAKGWVAHVISNPWLF
                     TSPGEGADWGSTMTGGAWLACQLWEHYLFSKDTLFLRQYYPVIREAAEFLADVMITEK
                     VHGWLVTAPSNSPENTYIMPGGFHGNTCMGPTMDNQIARQLFNAVIEASELLDIDKGL
                     REILADKVNRLPPTRISPTDGGIMEWLDDWPSAEPHHRHVSQLFGLFPGDEITPFDTP
                     ELAVAARKTLELRGDGGTGWSKAWKMCFYARLGDGAYTLKLLKALLKPAASGGGTYDN
                     LFDAHPPYQIDGNFGATAAIAEMFVQSHGREEIIRLLPALPDDKDWANGRISGLRARG
                     NYEISLEWNDHVLLNAVILAHNDGQCRIVLPENMIIYDEAGTEIPLTMTKDCFSFSVK
                     AGKKYFVKNSLR"
     gene            3917..>4482
                     /locus_tag="PLG24_10015"
     CDS             3917..>4482
                     /locus_tag="PLG24_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HMZ25270.1"
                     /translation="MMKLLNILILNLIALATMVAQTPVQIKVDGMTCDFCVNSVTKKL
                     SNAKEIENITVDLEKGLVHFNVKKGSKVDKKKYMELVEKAGYDAREIKVGNEIKATQN
                     AKAKQVNYVPASHETTTFKVGGECGMCKQRIESNVRAIKGVKSASWNKETKVLTVTYN
                     PMKIDIMDVHKKIASVGHDTELVKADDAV"
BASE COUNT         1305 a          888 c         1028 g         1261 t
ORIGIN      
        1 ccgaatctta taactatgcg ggtacctcag ttaattggaa tcatactgcc gagccgggag
       61 tttcgcacac catgaattat gtggatggct ttacaggaga ccccggaaca gttgatatcc
      121 tttaccctgt gaataatgtt tacaagggtg gtgccgtacg caatgaccgc ctcaccaaga
      181 tcatcaccca gaaatatctg gcacaggtac cttggttgcc gttagaagca tggaatgaca
      241 aacgaagact tggacttcca ttctttgaaa atccggctgt tgaagacgtt ctgcctaatt
      301 tgcctgactt gaacagctcc aactttatga ccagtagcat caagttttat gctcaaaggc
      361 ttaagtaccc atcctccctc tctaatagca gtaaggatgg atataaccag gcagtgagcc
      421 agttaggtgg acctgacaac gtattaaccc cattatggtg ggcaaaacaa aattaatgaa
      481 ggacgcgagt tttttgaata attgaagtgt actgaatatt ttttgggcgg gcagcgatgt
      541 ccgccttttt tatttctggc tatcatgtaa tgttgataat caatcaaatc aaaaggagga
      601 agatgcattt ctgcatttat tgtttgatac ttatgattag accggggtag tgaagctgct
      661 gagacgatag tcgccttaaa gtaactatat tcaagtcggc tgcctcaaag gtaagattca
      721 agttaagaca gttaagtcag tatcctaagg aattcattat atgattgagt atatattgca
      781 aatataaaat tatcaattca taaagtattg taaaaaatat aatataataa ttgtcagctt
      841 tttttcttta atggtctcgc cacatctgca ttcaatgagg tgacattctc tttggcctgt
      901 aatggtttca taaaagggga agttggcaaa gaacgtatga ttaggcttcc atcttggagt
      961 tcaaaacaga tttgccggat aagaaaatgc attcagagtt ttctatcttg cttatcagga
     1021 tcgaagtcaa aggcacagaa atgttgtaac tttttctaac ctcgcccata gtgagtcgtt
     1081 gagcaccttt gattttaaaa atgttagaca taggaagatc catcattatt gtgccagctt
     1141 tgaagagatt ttcaggtact atagtaagta agtcgtacac atctatactt tggacaaatt
     1201 ttatgggcaa ttgagtgcca ctggcgttgg aatgatataa tatacatttt ttgagcggat
     1261 aaattattat tttgtggcaa gtaaatgata gactgtaaat ttgtccggac tcaataaatt
     1321 tcaataaggt gaagagaata ctgattcctg tttttttgat tattattact tcaggattgc
     1381 tgtcttccca aactaatcag tcagaaatca ttttcgatag tccggctact cattttaccc
     1441 ggtctctccc tcttggtaat ggtcgacttg gtgccatgat tttcggagac cctcatcatg
     1501 agcgcatcgt actcaatgaa atctccttat ggtccggcgg gcctcagaat gctgaccgcg
     1561 acagtgcaag gtacttcctc ccggctattc gggatttctt acttaatggc gataatgcta
     1621 gcgctcagga attgctcaga agggagtttg tggcaaaggg agcgggttct ggtttcggca
     1681 atggtgcaaa tgtacccttc ggttgttacc agactgcagg tgaattgaga gtggactata
     1741 ataacatcgg aacggtaaca gactaccata gggtacttga tcttgaggtc gcaaaaagta
     1801 aagtttcctt cagggtcaat gacacaagaa tcatacaaga agcattttgt gatttttcgg
     1861 atgatgtcat tcgtatccgg tggcattctt ctgcaccggc agggttgaat tttagtcttt
     1921 ctttatttag aaaggaaaat gtctcatcgg taaattacaa gcatggcgta ctttctctgg
     1981 aaggcacgct tcccaatggt gaaaataaag gtatgtcgta tggcatccgg ctcaaagtaa
     2041 tcggatgtga tggaaatttc aacttttcta atggaaaatt gaatatttcc aatgccggca
     2101 catgctccgt catcattgca ataaggacaa attacaatta tgctgcagga gggctgttgc
     2161 cgtccactat tgtttcgaaa tccctgaaaa gtgaccttaa agcttcgacc tgccatgact
     2221 ttcatcgcgc atggatcaag tcatctgcta tttatcagaa atatttcaac gcgtgcaggc
     2281 tacaactgtt tccggatgaa aatcaaaaag tgaatagcct gaccactcca caaagattgg
     2341 aacagtatgc caatggagga tccgacgtcg gtctgccggt actttatttc aatttcggac
     2401 ggtacctgat gatttgttct tcgaagccgg gattattgcc caccaatctt cagggactat
     2461 gggcggaaga ataccaaacc ccatggaatg gtgattatca tctcaatatc aatctgcaga
     2521 tgaattactg gttggcagag acaactaatc tacctgcaca actcaaacca ttatttcaat
     2581 ggacaagccg cctggttccc aatgggagaa aaacagcgac cgcctattac ggtgccaaag
     2641 gttgggtggc tcatgtcatc agcaatccat ggttgtttac ctctccgggc gaaggtgctg
     2701 actggggttc gaccatgaca ggtggcgcct ggctggcttg tcaactatgg gagcattact
     2761 tgtttagcaa agacacgttg tttttgcgcc aatattatcc tgttatacga gaggctgcag
     2821 aatttttggc cgatgtcatg atcacggaaa aggtacatgg atggttggta actgcacctt
     2881 ccaattcacc ggaaaatacc tacatcatgc ccggtggctt tcacggcaat acctgtatgg
     2941 ggcccacgat ggacaatcag atcgccagac aattgtttaa tgctgtaatt gaggcatctg
     3001 aattattgga catagacaaa gggttgcgag aaatcttagc ggataaagta aacaggcttc
     3061 ctcctacgcg gattagtccg actgacggtg ggattatgga atggcttgat gactggcctt
     3121 cagctgagcc gcatcatcga catgtatccc agctgtttgg tctgtttccg ggtgatgaaa
     3181 tcacaccttt tgatacaccg gagctcgctg tcgccgctcg caagaccctg gaacttcgcg
     3241 gtgatggcgg tacaggttgg agcaaggctt ggaaaatgtg cttttatgcg cgactcggcg
     3301 atggagcgta taccctgaag ttgttgaagg cattactgaa acctgctgcc tccggaggag
     3361 ggacttacga caatcttttc gatgcacacc cgccatacca gatcgacggc aactttggag
     3421 ctaccgctgc cattgctgag atgtttgtgc agagtcacgg tagagaagag atcatccgcc
     3481 tgctacccgc tttgcccgat gacaaggact gggccaatgg aaggataagt gggctgaggg
     3541 ccaggggcaa ctacgaaatc agccttgagt ggaacgatca tgtcctgctc aatgctgtta
     3601 ttctggctca taatgatggg caatgccgca tcgttttgcc tgaaaatatg atcatctacg
     3661 atgaagcagg aaccgaaatc ccgctaacaa tgacaaaaga ctgtttctca ttttcagtaa
     3721 aggcaggaaa aaaatatttt gttaaaaata gcctgagata agccatttaa ccaatattta
     3781 tcaaaagcga catatttatg ttctaatttt gtgcgttcaa tctccggaat gaaacctttg
     3841 gcaaaatatt ttgttgtaat taaagaaaat ttgcgttttt tcaaaataat atttttaact
     3901 actcaaactt tattcaatga tgaaattact gaatatactt atcctcaacc tgatcgcctt
     3961 agcgactatg gtggcacaga ctcctgtaca aattaaggta gatggcatga cctgtgattt
     4021 ttgtgtcaat agtgtgacca agaaactttc caatgcaaag gaaatagaga acattactgt
     4081 ggatcttgaa aaggggcttg tacatttcaa tgtcaagaaa gggtcgaaag tcgataagaa
     4141 gaaatacatg gaattggtgg aaaaagccgg ctatgatgcc cgggagatta aggtgggaaa
     4201 tgagatcaag gcaacccaga atgccaaggc aaaacaagtc aattacgtac ctgcctctca
     4261 tgaaacaact accttcaagg tgggtggaga atgcggaatg tgcaaacaac gaattgaatc
     4321 caatgtgagg gcgataaaag gtgtaaaatc agcatcctgg aacaaggaga caaaagtttt
     4381 gactgtaact tataatccaa tgaagatcga tatcatggac gtacacaaga aaatcgcttc
     4441 tgtggggcac gatacggaat tggtcaaagc agatgatgct gt
//
LOCUS       DAOQQN010000275         4479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9042_length_4479_cov_4.964286, whole genome shotgun sequence.
ACCESSION   DAOQQN010000275 DAOQQN010000000
VERSION     DAOQQN010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9042_length_4479_cov_4.964286"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLG24_10020"
     CDS             <1..302
                     /locus_tag="PLG24_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25271.1"
                     /translation="MILIFVVFFATFITYLLNIFSLKYLSPSITGSYVYTQPALVILL
                     AHIFTFIGWTDDVTGSINSAKILFMTMIAIGVYLVSKPPTEGCSTTLAKKGKENN"
     gene            complement(310..1920)
                     /locus_tag="PLG24_10025"
     CDS             complement(310..1920)
                     /locus_tag="PLG24_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ25272.1"
                     /translation="MITVQNLALQYGKRVLFEDVNLKFTQGNCYGVIGANGAGKSTFL
                     KILSGEIAANRGLVQMEPGKRMAVLRQNHQEFDNYTAMDTVIMGHRKMYDIMIEKNNI
                     YMDPDATEADSLRAADLEAEFGEMGGWTAESDAGELLSNMGIPTALHTALMSELRGPD
                     KVKVLLAQALFGNPDYLLLDEPTNDLDALTVTWLSDFLADFRNIVIVVSHDRYFLDMI
                     CTHMVDIDFGAINIFSGNYTFWYETSQIMRRQTDINNKKVENKRQELMEFIARFSANA
                     SKSRQATSRKKALEKLNLEEIKPSSRRYPYIAFIPERQPGDILLKVDGLTKKDINGNV
                     LFDNISFTVNSDEKVGIICDRAVAMTAFFDVLAGKETPDAGTIEWGQTITYDYLPNDN
                     SAFFTGANDLTLVDWLRQYSEEKDEQFIRGFLGRMLFSGEEVFKKVSVLSGGEKVRCM
                     LSKIMLAHPNFLLFDDPTNHLDLESIITLNDAMKNFKGNIIFNSHDHQLMQTVSNRII
                     EILPMGTKESLSSYEEYLQQKKLQLAGA"
     gene            2227..3603
                     /locus_tag="PLG24_10030"
     CDS             2227..3603
                     /locus_tag="PLG24_10030"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HMZ25273.1"
                     /translation="MKNLKLFIVIILGLFIVGCTQDDVVVDKKLPLVSDFDYKVINDW
                     NTTFLRIERYADGYRPGPAPRGLAYLGLAAYEACIPGMPEYKSFSGYFPGFQVPAGDP
                     NAEYNWPLVVNETYHYLIPLFFSQATQQQLNAADDMYNSNLNRLKTNVPEEVVSRSIA
                     RAREVAEAVWNYAKSDPIGHEHYLHNTEGWSWQDNFKKDGDWKPTPPDFRLPVGGVWG
                     KARTFSLKSEAERLCPPPLPFSEATNSEFYAEALEVYSQNTPTISFEKEWISMFWHDD
                     FLNVTFSPACRWISVAMQVMEKENTNLRDAVVLLAKMGIAMNDASIACWYSKFYYNVE
                     RPVTYIQRVIDPNWEPHLYNAVTGEKGLTPPFPTYPSGHGSMGAAGAEILGSMFGYGH
                     SMTDYSHETRTDFSGMPRAYSSFLSMTQENAWSRIPLGVHFRMDAEAATNLGTAIGRK
                     VNALPWKK"
     gene            3781..4320
                     /locus_tag="PLG24_10035"
     CDS             3781..4320
                     /locus_tag="PLG24_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25274.1"
                     /translation="MIVLLIVLTTFYACKNETSEPAPASETTSDFRDGIQSNGVEVNP
                     YLNKEQDIKNNEGKVTRKVRFLPDGRKSVTDYYKGGDTVYLVRYFKNDLQDGKTIGYH
                     PNGKIREIQFFNMGKQFGQDSIFYETGELWYIYSFENDKKNGWMYRYSKDGKQEFAAN
                     YKDDKIIEVIDNLKPNKKK"
BASE COUNT         1314 a          983 c          890 g         1292 t
ORIGIN      
        1 ggatgatttt aatctttgtc gtcttctttg cgaccttcat tacctatctg ctcaatattt
       61 tttctttgaa atatttgtcc ccaagcatta ccgggagtta tgtatataca caacctgcac
      121 ttgtcatcct tttagctcat atcttcactt ttatcggttg gacggatgac gtcacaggat
      181 caatcaatag cgcaaaaatt ctattcatga ccatgattgc cataggggtg tatcttgtgt
      241 ccaaaccccc tacggaagga tgttcgacca cgcttgccaa aaaaggtaaa gaaaataact
      301 gaggctactt caggctcctg caagttgcag tttcttctgc tgaaggtatt cctcgtagga
      361 ggacaggctt tccttggtgc ccattggcaa tatctcaatg atcctgttag agacagtctg
      421 catcaactga tggtcatgac tattgaaaat gatattgcct ttgaaatttt tcatcgcatc
      481 attgagtgtg ataatggact ccaaatccag atgattagta gggtcgtcaa agaggagaaa
      541 attgggatgt gcaagcatga tcttggagag catacatcga accttctctc caccgctcaa
      601 cacactgacc ttcttgaaga cctcttctcc ggaaaacaac atcctgccca gaaaacccct
      661 aataaactgc tcatccttct cttccgaata ctggcgcagc caatcaacaa gggtgagatc
      721 attcgctccg gtgaaaaacg ccgaattgtc gtttggaaga tagtcatagg ttatggtctg
      781 accccactct atagtgcctg catccggcgt ttcctttcct gccagcacat caaaaaatgc
      841 agtcatcgct actgcacggt cacaaatgat gccgaccttt tcatcgctat tcacagtaaa
      901 actgatgtta tcaaacagga catttccatt aatatccttt ttagtcagac cgtccacctt
      961 cagaagaata tcacccggtt gtcgttctgg aataaaggct atataggggt atcttctcga
     1021 cgaaggtttg atttcttcca gattcagctt ttcgagcgcc tttttacgac tggtagcctg
     1081 tcttgattta gaggcattgg cgctgaatcg tgcaataaat tccattagtt cctgacgctt
     1141 attctcgacc tttttattgt tgatatcagt ctggcgccgc atgatctggc tggtttcgta
     1201 ccagaaggta taatttcctg aaaatatatt aatggcacca aaatcgatat ccaccatatg
     1261 agtacaaatc atatccagaa aatacctgtc atgcgaaact acgattacaa tatttctgaa
     1321 atctgccagg aagtccgaca gccaggtcac tgtcaatgca tccaggtcat tggtaggctc
     1381 atcgagcaaa agataatccg gattgccaaa cagggcctga gcaagcaata cctttacctt
     1441 gtcaggtccc cgcaactcag acatcaatgc agtgtgcaaa gcggtaggta tacccatatt
     1501 gctcagcaat tcacctgcat cactttccgc cgtccagcca cccatctcac caaattctgc
     1561 ctccaggtct gcagcgcgga gactatcagc ttccgtagca tccggatcca tataaatatt
     1621 gttcttctca atcatgatgt catacatctt ccgatgtccc ataatgactg tatccatggc
     1681 agtataattg tcaaattcct gatggttctg acgaagaacc gccattcgtt ttcccggctc
     1741 catttggact aaccctctat tggcggcaat ctcaccggac aggatcttca gaaaggttga
     1801 tttacccgca ccattagcgc ctatgacacc ataacaattg ccttgggtga atttcagatt
     1861 gacatcttca aaaaggactc ttttgccata ttgcagggct aaattctgga ctgtaatcat
     1921 gatgagattt tggtaagaag ccgcaaaggt ataaaaaata tacctgaata ttgcgtaatt
     1981 aggtattgac agaatgtgag attacaatat atttaatcct ggaatcgacc aactaacaca
     2041 atcatctctt tccatcaaat aatatactta aatctttagt aaatacagag aataaaaggg
     2101 ctaattataa aatgacaatg tcaaatttta ataacttttt aatgaatttt tcaaaataag
     2161 gccgggcaca ggtgtatatt tgttctttat attaaataac ctatcataac atacatttat
     2221 ttaaaaatga aaaatttaaa gctatttatt gtaatcatcc tcggcttatt catagtcgga
     2281 tgtactcaag atgatgttgt tgtcgacaag aaactgccat tggtctcaga tttcgactac
     2341 aaggtcatta atgactggaa cactactttc cttagaatcg aacgatacgc tgacggttat
     2401 cgtccaggac ctgctcccag aggtcttgcc taccttgggc ttgctgccta tgaagcttgt
     2461 attccgggca tgcctgagta caaatccttc agcggttatt tcccgggatt tcaagttccg
     2521 gccggtgatc caaatgcaga atataattgg ccattggttg tcaatgaaac ttatcattat
     2581 ctaattccat tgttcttctc tcaggccact cagcagcaat tgaatgcagc tgatgatatg
     2641 tacaactcca atctgaacag acttaaaacc aatgtacctg aggaagtcgt aagccgatcc
     2701 atcgccagag ctcgagaagt agctgaagct gtttggaact atgccaaatc cgatcctatt
     2761 ggacatgagc actacctgca taatactgaa ggttggagct ggcaagacaa cttcaagaaa
     2821 gatggcgact ggaagccaac accgcctgat tttcgccttc ctgtaggagg cgtatggggt
     2881 aaggcaagaa ctttttcctt gaaatcagaa gcagagagac tatgtcctcc acctcttcca
     2941 ttctctgaag ctaccaactc agaattttat gctgaagctt tagaggtcta ttcacagaat
     3001 accccgacta tttcctttga aaaagaatgg atatctatgt tctggcatga tgactttctc
     3061 aatgttacgt ttagtcctgc atgccgttgg ataagtgtag ctatgcaggt aatggaaaaa
     3121 gaaaatacca atcttcgtga tgccgtcgta ttgttagcca aaatgggtat agctatgaat
     3181 gacgcatcta tagcttgctg gtattccaaa ttctattata atgtcgaaag accggtgact
     3241 tacatccaaa gagtaatcga tcctaattgg gaacctcacc tctataatgc cgtcaccgga
     3301 gagaaaggac ttactcctcc tttcccaact tatccttcag ggcacggctc catgggcgct
     3361 gccggtgctg aaatcctggg cagcatgttt ggttacggcc actcaatgac tgactactcc
     3421 cacgaaacaa gaacagattt ctcaggtatg ccaagagcct attcaagctt cttgtctatg
     3481 acacaggaaa atgcatggtc gcggattcca ctgggtgtac acttcaggat ggatgcagaa
     3541 gctgctacca acctcggcac agctattggt agaaaggtca acgctcttcc ctggaaaaaa
     3601 taacttaatc gaaaaaagta ccccgccggg gtactttttt ttgccttaaa aataaatttg
     3661 tttaatacct cttttctcaa aggctcttct tagggataga aggttatttc ttaattgttt
     3721 accggatgat cggataaatt ttgttatctt gtaaagaaca ataaaattat tatggaaaga
     3781 atgattgtat tacttattgt gttgactact ttttatgctt gcaagaatga aacttccgaa
     3841 ccggccccgg cttcagaaac cacttccgac tttcgtgatg gaattcaatc caacggcgtc
     3901 gaagtaaatc cataccttaa taaagagcag gatatcaaaa acaatgaagg caaggtaacc
     3961 agaaaagtcc gattcctacc tgatggcaga aagtccgtaa ctgattatta caagggtgga
     4021 gatactgtat acctggtccg ttacttcaaa aatgacctac aggatggtaa gaccatcgga
     4081 tatcacccga atggtaaaat tcgggaaata caatttttca acatgggtaa acagtttggt
     4141 caggactcca tattctatga gaccggcgaa ctttggtata tttattcctt tgaaaacgat
     4201 aagaaaaatg gttggatgta tcgttactcc aaggatggga aacaggagtt cgcagccaat
     4261 tacaaggatg acaaaatcat tgaagttatc gacaacctaa aaccaaacaa gaaaaaatga
     4321 gtacgccaaa ttctttaacg ttattgatac gggagtctaa aacggatgtc ctggcaaaaa
     4381 taaaattata cctgcgtatt gctgtcggtg tttgtttcat cggtcatggt atgtggggca
     4441 tcgtatataa tgaggctgcc gattttcttc tggggaaag
//
LOCUS       DAOQQN010000276         4475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9058_length_4475_cov_4.936425, whole genome shotgun sequence.
ACCESSION   DAOQQN010000276 DAOQQN010000000
VERSION     DAOQQN010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9058_length_4475_cov_4.936425"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLG24_10040"
     CDS             <1..363
                     /locus_tag="PLG24_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HMZ25275.1"
                     /translation="GLPVLRMDVELKENELNMRKDMMAEGKAMLEAMGVKDAYEYDWG
                     YAPGMGIHEMGTARMGRDPKTSVLNGNNQVWDAPNVFVTDGACMTSASCVNPSLTYMA
                     MTARAADFAVKELKKMNL"
     gene            373..993
                     /locus_tag="PLG24_10045"
     CDS             373..993
                     /locus_tag="PLG24_10045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HMZ25276.1"
                     /translation="MERREMLKLIALYTGAAMIGGEFLLAGCKAPTGEDITFSDKVKS
                     LLNEVGETILPRTDTPGAKDADVAKVMEVVVRDCYWPEHQKVFVEGIRQIDEEAKGRF
                     GKTFMEISPEQRKELLIALEEEAKKFNEKVEEKDKPVREKMDKESKGYDFIASPRHYY
                     TMMKQLTMLAYFSSKECLTEAFEYIPVPGRYDGAYKIKPGDKLFSG"
     gene            1383..2183
                     /locus_tag="PLG24_10050"
     CDS             1383..2183
                     /locus_tag="PLG24_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25277.1"
                     /translation="MKDKNQWIEVVAFALPLLIASSLFFSCKQDKLVNDWTRMNLSGY
                     VMTIKEHSFKAIDTLGEIVQGERMSPSWRRDSYIVFNRAGNKVEENFYRNDGKMWSKS
                     VFSYDKNRKKIEEVVYRADRALWYTKITRFDSAGHIVEEEMFKPDNSLGVRMTFVYDG
                     SGNKIEEWKYTDNGKLSIRSVFKYDKNRNKIEEIMYNSEGGQIVRWVSKYDAKGRQIE
                     EDYYNAKNQLEARETYQYQFDSKGNWIQQIIFEDDSPKYVIRRELIYY"
     gene            complement(2267..2839)
                     /locus_tag="PLG24_10055"
     CDS             complement(2267..2839)
                     /locus_tag="PLG24_10055"
                     /inference="COORDINATES: protein motif:HMM:NF025013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HMZ25278.1"
                     /translation="MKHLLLYLSLIAVISCKTIEPNRSQKRVERRNERKANKGILDKE
                     MANTMVALADSRLMGISCSKLAQKKAGSEYYTTYASHAMENETFFLKEIKSISKSNNI
                     SLPRKIGAENEIKYDELKALSNKSFDKKYRKIEKKRLKEDRKLLNRLSASDDPIIREF
                     ATKWSAPVNEWRSNTEGTFVIAQFHWSIRL"
     gene            complement(2990..>4475)
                     /locus_tag="PLG24_10060"
     CDS             complement(2990..>4475)
                     /locus_tag="PLG24_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMZ25279.1"
                     /translation="REVTDYYKRKKMYLPYVKAMNQLGLAYSKINDDINARQCFIATL
                     EINNNILRDTHLILENKLLIIQNDIKTGNLDRALSLANHNERVAKRYGDRNLLAQTLF
                     QLGRINFKSDNFQKAETYLHIADSMMVETGSLQALPELYKILSQTYIELGKKEMLLPT
                     LEKYYSVVDKIKNTEIVKSSQEIAQRFDSENKDQMIKQLENENELKTINSRQQRIMIY
                     ILIFAFLATILAIYLIYRGYTSRLKSNQIIARQKSELQDKQLKQIEQEGQIKAMESML
                     IGQEAERNRIGKDLHDSLGAMLSTIKLQMSADANKNGSIDSPAMKSAKEMIDEACEEV
                     RKISRDMMPITLSKYGLHTALEELVDKYTFEGGPTVIYQSFGFNRVSDKDLDLFVYRI
                     IQELINNSIKHAEAQEIIVQINYLEDLMMITVEDDGKGFEYNSTEYSGMGLKNVEYRT
                     QYLKGKVTFDSGPGSGTIVVVEIPVTQFVKNKEVISGQTDPPRV"
BASE COUNT         1243 a          825 c          995 g         1412 t
ORIGIN      
        1 ggccttccgg tgcttagaat ggatgtcgaa ttaaaagaaa acgagcttaa tatgcggaag
       61 gatatgatgg ctgaaggaaa agccatgctg gaagctatgg gcgtcaagga cgcctatgaa
      121 tatgattggg gttatgctcc cggcatgggt atccatgaga tgggaactgc ccgcatgggg
      181 agggatccaa agacttctgt acttaatggt aacaaccagg tttgggatgc acccaatgtt
      241 tttgtaaccg atggggcttg tatgacctca gcttcatgcg tcaatccttc attaacctac
      301 atggcaatga cagcacgggc tgctgatttt gcagtaaagg aattaaagaa aatgaattta
      361 taggacaagg ctatggaaag acgagaaatg ttgaaattga tagcgctata tacaggtgct
      421 gccatgatag gcggtgaatt ccttttggcg ggctgtaaag cgccaaccgg agaggatatc
      481 accttttctg ataaggtcaa gtcgttgctc aacgaggtag gcgagactat ccttcctcgt
      541 acggatactc ccggtgccaa agatgctgat gtggcaaaag tcatggaagt agtggtacgt
      601 gattgttatt ggccggaaca ccagaaagtg tttgtggaag gcatcaggca aattgatgaa
      661 gaagccaaag gcagattcgg taagacattc atggagataa gccctgaaca acggaaggaa
      721 ttgcttattg cccttgaaga ggaagctaag aagttcaatg aaaaggtgga ggaaaaagat
      781 aagcctgttc gggagaagat ggataaggaa agcaaagggt atgactttat cgcttctcca
      841 cgacattatt atacgatgat gaagcaactg accatgctgg cttattttag ctcgaaagaa
      901 tgcctcaccg aagccttcga atatattcct gttccgggtc gttatgacgg agcttataag
      961 ataaaacccg gcgataaact atttagcggc tgatgttttt catgatttaa tgtttacgtc
     1021 agtaatgttt attttatttg atatgtaagt atctcatcgt cagtactcta ctaatgtttt
     1081 agccgtaggt ctttactttg tcttcgcgag gatgttgaat gctgtcaacc ggaatcattg
     1141 cctcccattt tgaacaggtt tgaatccaca tttgtttatc agtatgccgg cacggggtca
     1201 tgatttttag aaaatattga tgaacgtctt gaaaatgaca aatcaattta ttatgtttgg
     1261 cgcttatatg taataatggt ataagatttg cttcaggatt caaatatcaa tgcaatattt
     1321 ttaattatta attcagtaag aaataggata atattcggtt gcctgatttt aagtaagctt
     1381 aaatgaaaga caaaaatcaa tggattgagg ttgttgcctt tgctttgcct ctgctcatag
     1441 caagcagttt gttctttagt tgcaagcaag acaaattagt aaacgattgg accaggatga
     1501 atctttccgg atatgtcatg accattaagg agcattcatt caaggcgatc gacaccctgg
     1561 gggaaattgt tcagggagag cgtatgagtc ccagctggag acgtgattct tatattgtat
     1621 ttaatcgagc ggggaacaaa gtggaagaaa acttttacag aaacgatgga aaaatgtggt
     1681 ccaaaagcgt gttctcgtat gacaagaaca gaaaaaaaat agaagaggta gtgtataggg
     1741 ctgaccgcgc tttatggtat accaagatta ccagatttga cagtgccgga catattgtgg
     1801 aagaggaaat gttcaagccc gataattcat taggggtcag aatgaccttt gtatatgacg
     1861 gatcggggaa taagatcgag gagtggaaat atacggacaa cggaaaattg agtatcagat
     1921 ccgtttttaa gtatgacaag aacaggaata agattgaaga aatcatgtat aattccgaag
     1981 gtggacagat cgtgagatgg gtctcaaaat atgatgccaa gggtcggcag atagaggaag
     2041 attactacaa tgctaagaat cagctggaag cgagggaaac ttatcagtat caatttgact
     2101 ctaagggtaa ttggattcaa cagattattt ttgaagatga ctcgcccaaa tatgtcatcc
     2161 gcagggagct tatttattat taacccaata agctatttat ttttgtttta ggttatacca
     2221 agatgattta tataatagtc cataaatttt tggtataatg ccgaggttac agtcgtatag
     2281 accaatgaaa ttgggctata acgaatgtac cttcggtatt actgcgccat tcattgacag
     2341 gggcactcca ttttgtagca aattcacgaa tgattggatc atcggaagct gatagcctgt
     2401 tcaaaagttt cctgtcctcc tttaatctct tcttttcaat ctttcggtat ttcttatcga
     2461 aacttttgtt ggacagtgcc ttcaactcat cgtatttgat ctcattttca gcgccgattt
     2521 ttctgggtaa tgatatattg tttgactttg aaatggattt gatttctttc aggaaaaacg
     2581 tttcattttc cattgcatgg ctcgcataag tcgtataata ttccgaacct gctttttttt
     2641 gtgccagttt ggagcaactt attcccataa gccttgaatc agctaatgcc accatggtat
     2701 tggccatttc cttatccagt atccctttgt ttgcctttcg ctcatttctt cgttctacac
     2761 gtttttgcga tctgttgggt tcaatggttt tgcagcttat aacagcaatc aatgacaggt
     2821 ataggagtaa atgcttcact ttacaatggt ttgtataata aaccccgaaa gtgttcaatt
     2881 attgtttctt atccttgaaa attgtaagaa tggaaacaaa tcaatcagga gtattcaaaa
     2941 cattgtaaca cttattgccg ggtataaata ccttgatagc tgtgtgtcct tacactcgag
     3001 gtggatctgt ctgtccggaa atgacttctt tgtttttgac aaattgtgtt acaggaattt
     3061 cgacgacgac aatggtaccc gagcccggcc cggaatcaaa agttactttg cccttaagat
     3121 attgagttcg gtactcgaca tttttcagtc ccatacctga atattcagtg gagttgtatt
     3181 caaatccttt gccgtcatcc tcgactgtta tcatcatcag atcctccaga tagttgattt
     3241 gtacaatgat ttcctgcgct tccgcgtgtt ttatactgtt attgatcaat tcctggatga
     3301 tacgatacac gaagagatca agatctttat cactgacccg gttgaagccg aatgattgat
     3361 agatgacagt gggtccccct tcgaaggtat atttatcgac caattcctcc aaggcggtat
     3421 gcagaccgta tttggataag gtgatcggca tcatgtctct ggaaatcttt cgcacttcct
     3481 cacatgcttc atcgatcatt tcctttgcac ttttcattgc cgggctatct atactcccat
     3541 tcttattggc atccgcactc atttgcaatt ttatggtact caacatagct ccaagactat
     3601 catggaggtc ctttcctatt ctgttgcgtt cggcttcctg tccaatgagc atggattcca
     3661 tggccttgat ttggccttcc tgctctattt gtttgagttg cttgtcctgt aattcagact
     3721 tttgacgggc aatgatttga ttggatttta gcctgctggt atatcctctg tatatcaggt
     3781 agatagccag gattgtggcc aggaaggcaa aaatcaggat atagatcata attctttgct
     3841 ggcgggagtt gatggtttta agctcgtttt cattttcaag ctgtttgatc atctggtcct
     3901 tgttttctga atcgaaccgt tgggcaattt cctgacttga cttgacgatt tcggtattct
     3961 tgattttatc cacgacggaa taatatttct ccagtgtagg taacagcatt tccttcttac
     4021 ccagctcaat gtaggtttga gaaagaattt tgtataattc cggcaatgcc tgaagactgc
     4081 cagtctctac catcatggag tcggctatat gtagataggt ttcagccttt tgaaaattat
     4141 cggatttgaa attgatgcgc cccagttgaa aaagcgtttg agcgagaaga ttcctgtcgc
     4201 catacctctt tgcgaccctt tcattatgat tggccaggct tagtgcacgg tcaagattgc
     4261 ctgtcttgat gtcattttga atgataagca gtttgttttc gagaattagg tgcgtatccc
     4321 tgaggatatt attattgatt tcaagtgtgg caataaaaca ttgcctggca ttgatatcat
     4381 cattgatctt actataagcc agccccagtt gattcatcgc cttgacataa gggagataca
     4441 tcttctttct cttgtaataa tccgttacct ccctg
//
LOCUS       DAOQQN010000277         4454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9121_length_4454_cov_5.971130, whole genome shotgun sequence.
ACCESSION   DAOQQN010000277 DAOQQN010000000
VERSION     DAOQQN010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9121_length_4454_cov_5.971130"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..678)
                     /locus_tag="PLG24_10065"
     CDS             complement(7..678)
                     /locus_tag="PLG24_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HMZ25280.1"
                     /translation="MAVKMRLQRKGRKQAPFYHIVVADSRSPRDGKYIERLGSYNPMT
                     KPATIDIDRELAFSWLMKGAEPTDTVRAILRFKGVLVKKHLMRGVAKGAFTQEEADKR
                     WQAWIDAKETRISERREGAKAEKVAKHKSIHGEAKVKPAPVVEEVPVEEAPVVEETTA
                     TEEVPAVVETPVAEEAPAAETPATEESPATEEAPVAEASVEEIPAEETPAEENKEAPA
                     DDQNA"
     gene            complement(934..3120)
                     /locus_tag="PLG24_10070"
     CDS             complement(934..3120)
                     /locus_tag="PLG24_10070"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HMZ25281.1"
                     /translation="MFRFFSAILIFFLCTACGLSGFAQKKDLPVNFVNPLIGTAGHGH
                     VFLGANVPFGMVQVGPNNIGKGWDWCSGYHYSSKEILGFSHLHLSGTGIGDWGDILVL
                     PATDKPKTIAARKDDLSDGYGSPFDHSNEECKPGYYKIYLDRYKVTAEISATSRAAIH
                     HYRYDAGVNNPHILLDLSFGIGWDGPKDMDLRRLDDSTYIGHRLSKGWADDQRVFFAI
                     RFRNKLGKIEIFNDTSKNNMAHAAAFHPHLDTDGTVDFKVAISSVSTENALLNLVTEM
                     PHWNFNQYREQATQLWNEALSVVDYKADHKKKTIFYTALYHSFFFPSVYDDVNGEYRS
                     SDQMVRQGVTNTNYTVFSLWDTYRAQHPMLTILKPRMVDDLINTMLRIYQEQGKLPVW
                     HLSGNETNTMVGYSAVPVVVDAWRKGFRGFDSNLAYEAVRHSSLQGTDGIQYIRKLQY
                     IPADSVHEAVAKALEYAISDGCIALMAEGLGHEGDASYYNKRSKMYSEYYDPETRFMR
                     GKMADGTWRPGFSPFEAKHRENDYCEGNAWQYTWLAPQDVNGLIELLGGAARFEAKLD
                     SLFVVTGDMGENASVDISGLIGQYAHGNEPSHHIPYLYNYIDRQDKTATLVHRIMHEM
                     YTDKPDGLCGNEDVGQMSAWYILSAMGFYPVHPAEGIFQIGTPMMNEARIKLPDNKTF
                     IIKVKRKKAEDYLVKSMKLNGQPYTKNYIHYKEIMAGGILEFILGK"
     gene            3378..4241
                     /locus_tag="PLG24_10075"
     CDS             3378..4241
                     /locus_tag="PLG24_10075"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HMZ25282.1"
                     /translation="MVFLQIAKELIRDTDKEVVSVVYWMDKFSELVIGFIPRLILALL
                     LYILGTWLIRKALRILTKIFHRSHIDPSLRNFLIMLTRIILVILLLLTVISLLGINIT
                     SFAALLAGLGVALGSALNGALGNFAGGVTILVLKPFREGDLIEAQNHFGIVREIGIVY
                     TSILTKENKTVRLPNGALSTGVVINYTANESLCINLKIPLDGKLDIDSARKLAVDVML
                     SHPDVLKDPIPEVKITDVGKEGISFVLCPKIKIGEYDPSNPRQVETDYYNVYYGVQEM
                     VIKAFKSHQLS"
BASE COUNT         1161 a          979 c          934 g         1380 t
ORIGIN      
        1 tattaatcac gcattttgat catcggcagg agcctctttg ttttcctcag ccggagtctc
       61 ttctgccgga atctcttcaa ccgaagcctc ggctaccggc gcttcctcag ttgcgggcga
      121 ttcttcggtg gcaggggttt cagccgccgg tgcctcttca gctacaggtg tctctactac
      181 agccggaacc tcttccgttg ctgtggtttc ttcaactacc ggagcttctt caacaggtac
      241 ctcctcaact accggagctg gtttgacctt tgcttcgcca tgaattgact tatgctttgc
      301 aactttttca gccttcgctc cttcacgtct ctcagagatt cgtgtctcct tggcatcgat
      361 ccaggcctgc catctcttgt cggcttcttc ctgggtaaat gcaccttttg ctacacctct
      421 cataagatgt ttcttcacca atacaccctt gaatctcagg atagctctga ctgtatctgt
      481 cggctcagct cccttcataa gccatgaaaa agccaattct ctatcgatat cgatagtagc
      541 cggtttggtc attggattgt atgaacccag tctttcgatg tattttccgt ctcgtggaga
      601 gcgggaatca gcaacaacaa tgtggtaaaa aggagcttgt tttcttcctt ttctttgcaa
      661 tctcatttta actgccatgt ctgttaaatt ttttgttata aatatatgaa tatcaacccc
      721 atgtcctttg tcgagcaaac cacagggatt taacgaggcg caaagataag aatatttttt
      781 tatttcagag tatgggccgg attaatattt ttcctttata tagtaaaaat tactccaaca
      841 cctgattgtg cccctgccac cgaatcttta taaccggaat aatttaaata cattttcgcg
      901 tgaaataata ataatttcaa gcattccttt aaattattta cccaaaatga attccagtat
      961 accacctgcc atgatttcct tatagtggat ataattcttg gtataaggct ggccatttag
     1021 tttcattgat ttgaccagat aatcttcagc cttttttctt tttactttga tgataaaggt
     1081 cttattgtcc ggcaatttga tgcgagcttc attcatcatc ggtgtaccaa tctgaaatat
     1141 accttctgcc ggatgtaccg gatagaatcc catggccgac agtatatacc aggcactcat
     1201 ctgacctaca tcttcgttac cacaaagccc gtcaggtttg tcggtgtaca tttcatgcat
     1261 gatacgatgc accagtgtgg cagttttatc ctgacgatcg atataattat ataggtaggg
     1321 tatgtgatga cttggttcat tgccatgggc atactgacct atcaggcctg aaatatccac
     1381 actggcattt tcacccatat ccccggtcac gacaaaaagc gagtcaagtt tagcttcaaa
     1441 cctggctgcc ccccccagca attctattaa tccatttaca tcctgaggtg ccagccaggt
     1501 atattgccat gcattacctt cgcaataatc attctcccga tgttttgcct caaaggggct
     1561 gaagcccggt ctccaggtac catcagccat tttgccccgc atgaatcttg tttcaggatc
     1621 gtaatattcc gaatacatct tgcttcgctt attgtaataa gaagcatccc cttcatggcc
     1681 aagtccttct gccatcaaag ctatgcaacc atcgctgatg gcatattcaa gagcctttgc
     1741 aacagcttcg tgtacagagt ccgcagggat atattgtaat tttctaatgt attgaatccc
     1801 atctgtgcct tgaagtgagg aatgtcggac agcctcatag gcaagatttg agtcaaatcc
     1861 ccgaaaacct ttcctccatg catcgacgac aaccggcaca gcactatagc ctaccatggt
     1921 attcgtctcg ttgccggaca gatgccatac cggaagtttg ccctgttcct gatagatgcg
     1981 aagcattgta ttgatcagat cgtctaccat ccggggtttg agtatggtta gcatcgggtg
     2041 ttgagcccga taagtatccc aaagcgaaaa taccgtataa ttagtatttg taacaccttg
     2101 cctgaccatt tggtcactac ttctgtattc gccgttgaca tcatcgtaca ctgatgggaa
     2161 gaagaagcta tggtacaagg ctgtgtagaa tattgttttt ttcttgtgat ctgctttgta
     2221 atccacgacc gacaaagcct cgttccatag ttgcgtcgcc tgttccctgt attggttgaa
     2281 gttccaatgt ggcatttctg tcactaaatt gagcaaagca ttttcggtac tgacagacga
     2341 aatcgcaacc ttgaaatcaa cggttccatc tgtatcaaga tgaggatgaa aggcggcagc
     2401 atgcgccatg ttgttctttg aagtgtcgtt gaaaatttcg attttgccta atttatttct
     2461 gaagcggatg gcaaagaata cacgctggtc atcagcccac ccctttgata gtcggtggcc
     2521 aatatatgtg gagtcgtcca atcgcctgag atccatgtcc ttagggccat cccagccgat
     2581 tccgaatgat aggtcaagta aaatatgtgg gttattaacc cctgcatcat atctgtaatg
     2641 atggattgca gctcttgatg tggctgatat ttcggcggtt accttataac gatccagata
     2701 gattttataa tatccgggct tgcattcctc attgctgtgg tcgaagggag aaccgtaacc
     2761 atcgcttaga tcgtccttcc tggctgcaat agttttcggc ttatcggttg caggtaagac
     2821 cagtatgtct ccccaatcgc caattcctgt accactaaga tgcagatgtg aaaaaccgag
     2881 aatttccttg ctcgaataat gatatccgct acaccagtcc cagcctttgc caatattgtt
     2941 tggcccaacc tgaaccatac cgaaaggtac atttgccccc aggaatacat gaccatggcc
     3001 ggctgtaccg ataagcgggt taacaaaatt caccggtaaa tctttttttt gtgcaaatcc
     3061 cgacagacca caagctgtac agaggaagaa aattaatatg gctgagaaaa agcgaaacat
     3121 tcgattcata aaatttaatg aaaataatta ttgcaatatt aaggcatttc aacgggattg
     3181 cagcccggtt ggttaaaatt taaacaggct gagactaatt atggggcata ttttgtcccg
     3241 gattattctt gatacaaccc ggaggagatt ttggttagaa aactattgtt tcgttacatt
     3301 tagaattgaa tatattcgtg gttttaaata caaagttcaa agtatttttg tttttttaga
     3361 gtgaaattat taccaaaatg gtttttctgc aaattgcaaa agagttgatt cgagatactg
     3421 ataaggaggt ggttagtgta gtttattgga tggataagtt ttcggaactg gttataggat
     3481 ttattcccag gctgatctta gcattgctgt tgtatatttt ggggacatgg ctgattcgaa
     3541 aggctctcag aattttgacg aaaatttttc accgcagcca tatcgatcct tccctcagga
     3601 acttcctgat tatgttgacc aggatcatac ttgtaatcct gttacttttg accgtcatca
     3661 gtctgcttgg catcaatatc accagttttg ccgctttgct tgcaggcctg ggtgttgcgc
     3721 taggatccgc actcaatgga gcattgggaa attttgccgg tggagtcacc attttggttc
     3781 ttaagccatt tagggagggg gatcttatcg aggcccaaaa tcactttggc attgtcaggg
     3841 agattggcat tgtctatacc agtattctga ccaaggaaaa taaaacggtg cgactgccaa
     3901 atggtgcact aagtacaggg gttgtaatta actataccgc taacgaaagc ctttgtatca
     3961 atctgaagat tccacttgac ggaaagctcg atattgatag tgccaggaaa ttggctgtag
     4021 atgtcatgct aagtcatccc gatgtactta aggatcccat tcccgaggtt aagataacgg
     4081 atgtcggtaa ggaaggaatc agttttgtcc tatgtcccaa aataaagata ggtgaatatg
     4141 acccatcaaa tcccaggcag gttgagactg actattacaa tgtttattac ggtgtgcagg
     4201 aaatggtaat caaggctttc aaatcgcatc agctatcctg aaatcttgaa tgagggtcta
     4261 ttaagaaagt gaggatgaat caggggtgga atcgaagtag gtagatgatt aatagccacc
     4321 atgcttgaca gtactgcctt tcggggtggc tttgccaaaa ttcagatttt tcattcggga
     4381 attaaatttt tcatttgctt ccaaatcctt gtgtatggcg gtttttgaat tattactttc
     4441 ctacctactt cgat
//
LOCUS       DAOQQN010000278         4431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9208_length_4431_cov_5.314899, whole genome shotgun sequence.
ACCESSION   DAOQQN010000278 DAOQQN010000000
VERSION     DAOQQN010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9208_length_4431_cov_5.314899"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1428)
                     /locus_tag="PLG24_10080"
     CDS             complement(<1..1428)
                     /locus_tag="PLG24_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HMZ25283.1"
                     /translation="MAKKQLIQVVRELSVGMDTIHDFLVSKGFELEKKPNAKLTDEMY
                     DALLIEYQNDLAIKNKADQMGLSRSSTKKEEKHEPASIEKNPPIVKKEEPVQTEELKP
                     VEDSVPNVSHEEYKPELKIVGKIDLDDLKPKKKIVESPKIESEELPVEVQDEVREKEE
                     TDVEATSGEQAAPDTHPAEQTQHEEDMYRAKSPTLKGLKILGKIDTDKIAKPVPKKKE
                     DHKKEKSGTQAPSNQNTSQSASDEHKRKRKRKKLPVNLSSESGNFHGHDGKGGDARQG
                     RGKKVNPEVVKEVSQKEIDEKIKSTMARLNVGGKNKRAKIRRDKRETIREKNEAMELA
                     SIDDKIQVTEFISVSELASLLNVPAIQVITTCMNLGVMVSINQRLDAEIIELVASEFG
                     KEIEFISAEDDTDFDTQEEDDPEEMLVERAPIVTVMGHVDHGKTSLLDYIRNANVVSG
                     EAGGITQHIGAYEVMVNGKKIVFLDT"
     gene            complement(1563..2801)
                     /gene="nusA"
                     /locus_tag="PLG24_10085"
     CDS             complement(1563..2801)
                     /gene="nusA"
                     /locus_tag="PLG24_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HMZ25284.1"
                     /translation="MNLTETFKDFKEGKNIDRPTMMRVLEDVFRTLIKKRYGSDENFD
                     VIVNMNTGDLELWRRRTIVPDGEVTDELTEVSITEALELDADYEVGDECNEKIDTDEF
                     GRRAVMAARQTLVSRIMELEKDEIYKRYEDRIGDLVSGEVHQILGKEVLVLDDATGQE
                     LVLPKTEMIRGDYFRKGESTRGVIKKVEMRNNLPVITLSRTEPLFLERLMEQEVPEIE
                     DGLITIKKIVRIRGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDIVN
                     FTTNTILYIQRSLTPAKITDIQVSPETMKAAVYLKADQVSLAIGKNGSNIKLASKLTG
                     YDIDVYRDSDETEVDDVELDEFADEIDGWIIDALKAIGCDTARSVLRLTVEELVRRAD
                     LEEDTVNEVIRILEAELEDE"
     gene            complement(2823..3296)
                     /locus_tag="PLG24_10090"
     CDS             complement(2823..3296)
                     /locus_tag="PLG24_10090"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25285.1"
                     /translation="MSLEEQIKKIVEDHFDLLGRQDCFLVDIIQKAKKLEIYIDSDDA
                     IDFDICKGLSRVTEQFLDESLLMGDDYILEVSSPGLSRPLKLPRQFKKNIGREVTMTN
                     SDKTKTVGILTQADDENFQITRSVTRKEGKKKITEDLITSVPYTEGRKVLINIKF"
     gene            3702..3887
                     /locus_tag="PLG24_10095"
     CDS             3702..3887
                     /locus_tag="PLG24_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25286.1"
                     /translation="MDKKQPPEEKRVPFYRMIMDDFMLLMFLGVTIYAIFYLLWGVME
                     LSNLPAIPDALKKSLLK"
     gene            3914..>4431
                     /locus_tag="PLG24_10100"
     CDS             3914..>4431
                     /locus_tag="PLG24_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25287.1"
                     /translation="MSLLSPAEGWYYKKVSKDEKMWMLIALVLCIMLFVWMVMWHVYG
                     KQNPSSTTYKTSTVEFTELTEAYTKQNMIGQDNGIPVVRPEPNSDVFLMAEMWRWSPV
                     LVLEINQAYRMHISSKDIVHGLSIQPVNMNFQVYPSYDYVLEFKPTEAGEYRVVCNEF
                     CGIGHHTMIGKIV"
BASE COUNT         1102 a          994 c          809 g         1526 t
ORIGIN      
        1 agtatcaagg aaaactattt tctttccatt taccatcact tcataggcgc cgatatgctg
       61 tgtgatacct ccggcttcac cgctgacgac atttgcattc cgtatatagt ccagcaaaga
      121 ggttttaccg tgatccacat ggcccatcac agtgacgatc ggagctctct caacaagcat
      181 ttcttccgga tcatcttctt cctgagtatc aaaatcagtg tcatcttcgg cgctgatgaa
      241 ttctatttcc ttgccgaact cactcgcaac cagttctatt atctctgcgt caaggcgctg
      301 gttgattgat accatcacac caagattcat acatgtggta ataacctgta tcgcaggaac
      361 attgagcagt gatgccaact ctgacacgga gatgaattcg gtaacctgga ttttgtcatc
      421 tatgctggca agttccattg cttcattttt ctcccggatg gtttctctct tatccctgcg
      481 tattttagca cgtttatttt ttcctccgac attgaggcgt gccattgtac tcttaatctt
      541 ttcgtcaatc tctttttgag atacttcctt gacaacttca ggatttactt ttttgcctct
      601 gccttggcgt gcatcacctc ctttaccatc atgaccgtga aaattacctg attctgaaga
      661 aaggttgaca ggtagttttt tgcgtttgcg tttccttttg tgttcatcag atgctgattg
      721 tgaagtattc tggttggatg gagcctgagt tcctgacttt tctttcttat ggtcttcctt
      781 ctttttcgga actggtttgg ctatcttatc agtatcaatt tttccaagta tcttcaatcc
      841 tttcagggta ggagacttgg cccgatacat atcctcttcg tgttgagtct gttctgcagg
      901 gtgtgtatcc ggtgctgcct gttcacctga ggtagcttcg acatcggttt cttctttctc
      961 cctgacttca tcctgtactt ctacaggcag ttcttcactt tctatctttg gagattcaac
     1021 gattttcttt ttaggcttca gatcatccag atctattttt ccgacgatct ttagttccgg
     1081 cttatattct tcgtgagata cattggggac agaatcttca acaggcttca actcttctgt
     1141 ttgaaccggt tcttcctttt tgacaatcgg cggattcttt tcgattgagg ctggttcgtg
     1201 tttttcttcc ttctttgttg aagatctcga caaacccatt tgatctgcct tgtttttaat
     1261 agccagatca ttctgatact caatcagtaa tgcatcatac atttcatctg tcaatttcgc
     1321 attgggtttc ttttccagtt caaaaccttt tgatactaaa aagtcgtgaa tggtatccat
     1381 ccccacgcta agttctctga ctacctgtat taattgtttt tttgccattc agaataattg
     1441 ttccggtctg ctttctttac tactatttgt caaccggaaa tttgcacaaa agtagattaa
     1501 aaattaaaat caaaattaag attggcaaaa aactttaatg ccaatctcag gctattcccc
     1561 acttattcat cttccagttc tgcttccagg atgcgtatca cttcatttac agtgtcttct
     1621 tccaggtctg ctcttctaac caattcttca actgtaagcc taagcacgct tcgtgctgta
     1681 tcacaaccta tggcctttag tgcgtcaatt atccagccat cgatctcatc agcaaattca
     1741 tccaattcga catcatctac ctctgtttca tcagaatctc tgtacacatc aatatcgtat
     1801 ccggtcagct tggaagcgag cttgatgttg cttccgtttt tgccgatagc cagggaaacc
     1861 tgatccgctt taagatatac agctgctttc atggtttccg gtgatacttg aatatcagta
     1921 atcttcgcag gtgtaagtga cctttgaatg tacaatatgg tgttggtagt gaaattgacg
     1981 atatcaatgt tttcgtttct caattcccta actataccat ggatacggct tcccttcatc
     2041 cccacacagg caccgaccgg atcgattcgg tcgtcatagg attcgactgc gactttagcc
     2101 ctttcaccgc gaattctcac aatcttcttg atggtaatca gtccgtcttc aatttccggt
     2161 acttcctgtt ccattagtct ttccagaaat aatggttctg ttcttgacaa ggtaatgaca
     2221 gggaggttgt tgcgcatttc gactttcttg atcacaccgc gtgttgactc ccctttgcga
     2281 aaatagtccc cacggatcat ttctgtcttt gggagtacca attcctgccc cgtggcgtca
     2341 tccaggacaa ggacttcctt gcccagaatc tgatgaactt cacctgacac cagatcgcct
     2401 attctatctt cgtagcgctt atatatttca tccttttcca gttccatgat acgggatacc
     2461 agtgtttgtc ttgctgccat gactgcacgc cttccgaatt catcggtatc tattttttcg
     2521 ttgcactcat cacctacctc ataatctgca tccaattcga gcgcttctgt gatggatact
     2581 tctgtcaatt cgtccgtcac ttcaccatca gggacaatag tcctccttct ccaaagctca
     2641 aggtctccgg tattcatatt tactatgacg tcgaagtttt catctgaacc atatcttttt
     2701 ttgataagtg tgcggaatac atcctccaat acacgcatca tggtagggcg gtcaatattt
     2761 ttaccttcct tgaaatcctt aaaggtttct gttaagttca tttttgaaaa ttataatatg
     2821 gtttaaaatt taatgttgat cagtactttt cgtccttcgg tataaggcac tgaggtaatc
     2881 aggtcttctg taatcttttt tttaccctct tttcgggtga cacttctggt aatctgaaaa
     2941 ttttcatcat ccgcttgtgt caatatcccg actgtttttg tcttatcgga attggtcata
     3001 gtgacctctc ttcctatatt tttcttaaac tgacggggta acttcaatgg tctgctaagc
     3061 cctggcgaag acacctccaa aatgtagtca tctcccatca ataagctttc atcaagaaat
     3121 tgttcggtca ccctgctcag acctttacag atgtcaaagt caatggcatc atccgagtca
     3181 atatatattt ccagtttttt agccttttgt ataatatcga ctaagaagca atcctgccgc
     3241 cccaatagat cgaaatgatc ttctactatt ttttttatct gctcttccaa tgacattgtt
     3301 atataaaata aaatagggaa cttccgctcc ctaccaattg cttgcaatct gacacaaagg
     3361 taagcattaa ttatcaaacc gccaaatttt ttatttaact attgcaatga gtatcaatgg
     3421 gctatggcca atcatcgctt gagttgttag ctcccttatg ctgacaggcc tttttagttt
     3481 tgccgacaac tcaaaatata atatgatcag tagataagtt ttaaatataa tattttgcaa
     3541 ttaactcctt ataacaaaaa tcagtaaatg tcattacggg gtaaaaagta cgaaaacgta
     3601 ttttttgaat atttaatcca aaggattctt tttttcttga aaaagtgctg aatacatttg
     3661 ttaacagatg tttaaattaa tttacctaaa atcgaaaatg tatggataag aagcaaccac
     3721 cggaagaaaa aagagtgccc ttctaccgta tgatcatgga tgacttcatg ttgttgatgt
     3781 tccttggggt taccatttat gctatttttt acctgctttg gggagtaatg gaattgtcaa
     3841 accttccggc aattccggat gcgctgaaaa aatccctgtt gaaataattt cgtaaaactt
     3901 aaaatttcta cttatgagct tacttagtcc agctgaaggt tggtattata aaaaggtatc
     3961 gaaagatgag aaaatgtgga tgttgatagc gctcgtgctc tgtatcatgc tttttgtatg
     4021 gatggtcatg tggcacgtat atggtaagca gaatccatcc agtacaacct acaagaccag
     4081 taccgttgaa tttaccgagc ttacggaagc ttataccaaa cagaatatga tcggtcagga
     4141 taacggaatc cctgttgtcc ggcctgaacc caactcggat gtatttctga tggcggagat
     4201 gtggcgttgg agcccggttt tagtcttgga aatcaaccag gcataccgca tgcacatttc
     4261 ttccaaggat attgtacacg gcctttccat tcaaccggtt aatatgaact ttcaggtgta
     4321 tccttcctat gattatgtgc tggaattcaa acccaccgaa gcaggtgagt atcgggttgt
     4381 ttgtaatgag ttttgtggca taggtcacca tactatgatt ggcaagatag t
//
LOCUS       DAOQQN010000279         4428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9219_length_4428_cov_6.172650, whole genome shotgun sequence.
ACCESSION   DAOQQN010000279 DAOQQN010000000
VERSION     DAOQQN010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9219_length_4428_cov_6.172650"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="PLG24_10105"
     CDS             complement(<1..1156)
                     /locus_tag="PLG24_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25288.1"
                     /translation="MSKFYKSLQRDYSFYSLQQCGVSLPLQWITPKMKLLGIVILLVL
                     SLNIGTAQISIQVGNTCNENFYYAHPSTADTLPLYWRIDSTAIVRTIDPYSKAGKRAP
                     RLAGNSMSSSSPNGLYNFGAGNPGLATDRAFGGLSSSTGCQSVNLYVYLKNTGSTSIT
                     DFTISYDVEKYRKGSNAAGFRVQLYYSYNGVSWIDAGSNFLSSFPADPDNLGYVTAPG
                     DSIIVNSQTLTLGTPLASDSLIYFAWNYSVTSGTTVTNAQALGVDDIVITANSNPLPV
                     NLIAFESKKVSNTEVLISWDVAEEYDLLGYVVERSNNGRGFKSIGQVHAQQRNSYFFR
                     DVNPFSGGNYYRLKIMDNNGASRYSDVQYVVLKKPETLTLYPNPTKGRIFI"
     gene            1670..2590
                     /locus_tag="PLG24_10110"
     CDS             1670..2590
                     /locus_tag="PLG24_10110"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HMZ25289.1"
                     /translation="MKKRLLILSYIFSILTSISGQNQQITVGILPTTDERGMTNSTTT
                     IISESIINCFSKTKRFQLVDRSKMEAINAEKDLQKTENFIDGTTIKQGNSVGAQYLVT
                     SSVSSFTSDKKDCSFLLNLKVINVETGQIRAAEDYSIKGMAIIAKTGSIVSGTTMLAP
                     IRGLGNIAGTNQAIGAIQSGNEDAAFKKALENMVEKVDEFVNKNFVPVYTLVKILKKD
                     SGGNAQQVLLLEGFDAGLKKGDRLKVDEITQLEVNGKSYTRKNTIAQLIIRNVEGENF
                     SACEITSGSKELTEKIEQNAIIQVSPLRKD"
     gene            2668..3582
                     /locus_tag="PLG24_10115"
     CDS             2668..3582
                     /locus_tag="PLG24_10115"
                     /inference="COORDINATES: protein motif:HMM:NF040342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6175 family protein"
                     /protein_id="HMZ25290.1"
                     /translation="MNLKKKFHLITLSLAFLSFINNQLAGQNDSSQPTEKISDVQPTI
                     MVIPFVQKGQDLRTVLEDDINLRIAITKVKEAFDLRGYTTIDFTAKLKAMKDNNIFLT
                     DNQSDLKSKIIEMSGSDIYVQTEVNVIGSNTGNTVTVILTAYDASTGNSLANKVGESG
                     KYYTENIGKLASKAVDSIADDFLKTMNTGFRNLVENGKSILIDISLSEDSDYKLSSVI
                     GSSELPLSDEIEVWISENAFKNYYHIQGVTDVKMIFDHVKIPVKDPETGLNYNTNKFA
                     LKFYLFLRKMGLNPKKDVKGNTIYITIN"
     gene            3588..>4428
                     /locus_tag="PLG24_10120"
     CDS             3588..>4428
                     /locus_tag="PLG24_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25291.1"
                     /translation="MKRILISLIFLSAIHTVFSQENEGNKIALSVVMPDNVEGLSASQ
                     LSKLKTKVISIVTANGLAATGIHNNFVIYPIFSVYDKNAVEGGMENIYVTTCELSLYI
                     QHTEDNIVFASYGKKFKGSGESIETAITNSISKIPQNSKEITEFIETGKQKIIVYYEG
                     KCEDFKKQAESLTKMQKYEEAIALLQSIPEEISCYQQMVEKSVEVYKAYQNKVCGELL
                     QKAKAQISIKNYKGAMEFLGQIDPSSSCPGESRRLMQSISNHLDAVAKQRWEHKMKVY
                     NDNL"
BASE COUNT         1592 a          755 c          803 g         1278 t
ORIGIN      
        1 atataaaaat cctcccttta gttggattag gatatagcgt caaagtttcg ggtttcttta
       61 aaacaacata ctgcacatca ctatatctgg atgcaccatt gttatccatt attttcaaac
      121 ggtaataatt tccacctgaa aatggattaa catcccggaa aaaatatgaa ttcctttgtt
      181 gagcatgtac ctgtccaatt gacttgaagc ctctgccatt attgcttctc tccacaacat
      241 agcccaataa atcatattcc tctgcaacat cccaggagat caacacctca gtattgctta
      301 ctttttttga ttcaaaggct atgagattta cagggagagg atttgagtta gctgtaatga
      361 cgatatcatc cactcctaat gcctgagcat tggttacagt agttcctgaa gtaaccgaat
      421 aattccatgc aaaataaatc agtgagtcag aagcaagtgg agtacccaat gttaatgttt
      481 gcgaattaac gataatggaa tcgcccggtg ccgtgacata acctaaatta tccggatcag
      541 ccgggaaact tgataaaaaa ttacttccgg catcaatcca gcttacccca ttataagagt
      601 aatatagttg aactctaaac ccggctgcat ttgaaccttt cctatatttt tcaacatcat
      661 aggaaattgt aaaatcggta atacttgttg aacctgtatt ctttagatat acatacaaat
      721 taacactctg acaaccggta ctggaggaaa gtcccccaaa agctctatct gtggccaggc
      781 ccggattgcc tgcaccaaag ttatacaaac catttggtga agaactactc atactattac
      841 ctgctaaccg gggcgcgcgc tttccggctt tggaataagg atcaatggtt cttacaatag
      901 cagtcgaatc aatcctccaa tatagaggca acgtatcagc agtagatgga tgggcataat
      961 aaaagttttc attgcaggta ttgccgacct gaatactgat ttgtgcggta ccgatgttca
     1021 gtgaaagtac caataaaatg acaatgccca aaagcttcat tttaggtgta atccattgta
     1081 gaggtaatga cactccacat tgctgcaaag aataaaatga ataatctctt tgcaaagact
     1141 tgtaaaactt agacataata atttagttaa atttaagaga tagttaaaaa atgagaggta
     1201 tatatatttt tacattttac aattaaacta attattattt aaaatgaagg tttcataatc
     1261 aaatcacggt aaagttaatc ctttatcatt aaaaaataat aaatttttac atattaaata
     1321 atattatcat gtgttatgat tttctttatt tcaaataaat agctgatcat ctaaaaaatc
     1381 gacaaatcaa ttactatttt attaaaatta cccataagaa tttgcctata acccacatta
     1441 aaaaatttat tttacaaaat aggcataagc tgatagatga gtataagcaa ttgacaaata
     1501 atatattaaa atcaataatt tcagtttaaa cctgtttata actgatttat tttagccttt
     1561 aagttatgtt tggccgagtt tttagttcca cagttttttc gaaacgaata aaatagcaat
     1621 ttgatcctct taggcaaaac gatttaataa taatagttaa tagagataaa tgaaaaaacg
     1681 gctacttatt ttatcttata tttttagtat actgacttcg atcagtggcc aaaatcagca
     1741 aatcacagta ggcatactac caactactga tgaacgaggc atgaccaact caacgacaac
     1801 tataatttct gaaagtataa tcaattgttt ttctaaaaca aaaagatttc aactggttga
     1861 caggtctaag atggaggcga taaatgcgga aaaagatctc caaaagacag aaaactttat
     1921 tgatggcact accattaaac aaggtaacag cgtcggagct caatacttag ttaccagttc
     1981 ggtcagtagc tttacaagtg acaagaaaga ttgttctttt cttctaaacc tgaaggtaat
     2041 aaatgtagag accggacaaa tcagagcagc agaagattat tcgattaaag gaatggccat
     2101 cattgcaaaa acaggatcga tagtgtcggg aacaacaatg cttgctccta tcagagggct
     2161 aggaaatatt gccgggacca atcaagcaat aggcgccata caatccggta atgaagatgc
     2221 ggcattcaaa aaagcattgg aaaatatggt agaaaaagta gatgaatttg tcaataagaa
     2281 ctttgtccct gtctatacat tagtaaagat tctaaaaaag gattccgggg gaaatgcaca
     2341 gcaggtattg ctgttggaag gttttgatgc aggattaaaa aaaggggata ggttgaaagt
     2401 tgacgaaatt acccagctgg aagtgaacgg caaaagttat acgaggaaaa atacgattgc
     2461 acaacttata ataagaaatg tcgaaggcga gaatttttca gcctgtgaga ttacttccgg
     2521 ttccaaagag ttgactgaaa aaatagaaca aaatgctatt attcaggtaa gtcctttaag
     2581 aaaggattaa actataaaat tcagaaattc tatctaaatc cattttgatt caactctttt
     2641 aatttctcac ataaaccatt tccctgaatg aatctgaaaa aaaaatttca tctgattacc
     2701 ctttcgctgg cctttctttc ttttataaat aatcaattgg cagggcaaaa tgattcgtct
     2761 caaccaaccg aaaagataag cgatgtacaa cctacgatca tggtcattcc tttcgtccag
     2821 aaaggtcagg atctacgtac ggtattggaa gatgatatta atcttaggat agcaattact
     2881 aaagtaaagg aagcattcga tttgcgtggc tatactacaa ttgacttcac ggctaaatta
     2941 aaagccatga aggataataa tattttcctt acggataatc agtctgattt aaagtcaaaa
     3001 attattgaaa tgtctggttc tgatatttat gttcagacag aggtcaatgt cattggatca
     3061 aataccggaa atactgtaac agtaatacta actgcatacg atgcctctac aggcaactca
     3121 ctggcaaaca aagtaggaga aagtggcaaa tattatacgg aaaatatcgg caaattagca
     3181 tcgaaagcgg tagatagtat tgcagatgat tttttaaaaa caatgaatac aggattccgg
     3241 aaccttgtgg aaaatggtaa gtcaattctc attgatataa gtctatcaga agattctgat
     3301 tataaattgt cttcagtgat tggctcttca gaacttccac tatccgatga aattgaagta
     3361 tggattagcg agaatgcatt taaaaattac taccatattc agggagtcac tgatgtaaaa
     3421 atgatttttg atcatgtaaa aattccggtc aaagaccctg aaaccggatt gaattataat
     3481 acaaataagt ttgcactcaa attttatctt tttttaagga agatggggct taatcccaaa
     3541 aaggatgtaa aaggaaacac catctatatt accattaatt aattagcatg aaaagaatcc
     3601 tgataagcct tattttcctg tctgctatac atacggtctt ctcccaggaa aatgaaggga
     3661 ataaaatagc tctttcagtt gttatgcctg ataatgtgga aggactttct gcatctcagt
     3721 tgagcaaatt aaagaccaag gttataagta tagttacggc aaatggattg gccgcaacag
     3781 gtatccataa taactttgtc atatatccaa tattttcagt atatgataaa aatgctgttg
     3841 aaggcggaat ggaaaatatt tatgtaacta cctgtgagtt aagtctctat attcaacata
     3901 ccgaagacaa tatagtattt gcaagctatg ggaaaaagtt taaaggaagc ggtgaaagta
     3961 tcgagactgc cattacaaat tctatttcca agattccaca gaatagcaag gagattacgg
     4021 aatttattga aacaggaaaa caaaaaataa tagtctatta cgaaggtaaa tgcgaggatt
     4081 tcaaaaaaca ggctgaaagt ctgaccaaga tgcaaaaata tgaagaggca atcgccttgt
     4141 tgcaatccat ccctgaggaa atttcctgtt atcagcaaat ggttgaaaag tcagtagaag
     4201 tatataaagc ctatcagaat aaagtctgtg gagagttgtt gcagaaggcc aaagcacaaa
     4261 tatccattaa gaactataaa ggagccatgg agtttttagg gcagatagac ccctcatcat
     4321 cctgccccgg tgaaagccgg agactcatgc aaagcatcag caaccacctg gatgctgtag
     4381 caaagcaacg atgggaacac aagatgaagg tgtataacga caaccttg
//
LOCUS       DAOQQN010000280         4428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9220_length_4428_cov_5.165790, whole genome shotgun sequence.
ACCESSION   DAOQQN010000280 DAOQQN010000000
VERSION     DAOQQN010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9220_length_4428_cov_5.165790"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PLG24_10125"
     CDS             <1..574
                     /locus_tag="PLG24_10125"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HMZ25292.1"
                     /translation="DVLGSAKGWAGAFLYNEKARSALERFFAREDTLSVGVCNGCQLF
                     IELGLINPDHEVKPKMRHNTSEKHECIFTTLTIGDNNSVMLSSLAGSTLGVWVSHGEG
                     RFSLPYEENKYHIVAKYAYDTYPASPNGSDYNTAMMCDVSGRHLVMMPHIERSFFQWN
                     WPYYPEGRNDEVSPWVEAFVNARKWIEARE"
     gene            677..2119
                     /locus_tag="PLG24_10130"
     CDS             677..2119
                     /locus_tag="PLG24_10130"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HMZ25293.1"
                     /translation="MRKILLFVSFALAVLIGILTFNYFSISSRQTAPTHEKADISSIV
                     GAEERLSRAITFPTVSFEDSTDADYTPFVAFHKYLQSTFPAIYENLEVENINEFSLLF
                     HWKGSDSNLKPVILMAHQDVVPVSEDSKNLWTVHPFEGTIKDGFLWGRGSIDDKSNLM
                     GQLEAVNFLLGLQFRPRRDIYLAFGHDEELGGENGAMKIAQLLKSRGIEAEFVLDEGG
                     IVTDNMVPGVRQPVALIGTSEKGYLDLTLSVRIEGGHSSMPKKDNAIGVISKALTNLS
                     DHPFPPQLSPSVRDFFSYVGPHSSFINRLAISNMWLFKPVLFKMIGKSAPGDAMIRTT
                     MVPTIIHGGDKSNVVPDIATANVNLRLLPGTTIPDAIDHVRKVINNPAVKIEALGEQK
                     EAGIVSPADNDAFKYISDAVHKNFPNTLVSPFLMIGGTDSKHFEILSQNIYKFSPLID
                     PIGFHGVDERLNLKAYPQAIGFYVDLIKSL"
     gene            2187..2585
                     /locus_tag="PLG24_10135"
     CDS             2187..2585
                     /locus_tag="PLG24_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25294.1"
                     /translation="MDWHPVLILRILMAAFSEFLTLVNLIKKRGVNKNLKKISAGVSE
                     EKNTYIDEFPEPIKSVAMTIAEQLYRKGVKEGKEQGVQEGLKKGLKQGIEKAKFEIIV
                     KLFENGASIGFITNITGLPENKIKEILNIQ"
     gene            complement(3268..>4428)
                     /gene="dnaB"
                     /locus_tag="PLG24_10140"
     CDS             complement(3268..>4428)
                     /gene="dnaB"
                     /locus_tag="PLG24_10140"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HMZ25295.1"
                     /translation="IEHHARIVVQKYIQRELINVSNQIIQDSYEDTKDVFDILDEAEQ
                     NLYRITDNNLNNGPQTFTAMANQVRLRMEEMASRPEGLTGVSTGFRELDKLTSGWQPS
                     DLVIIAARPSVGKTALVLSMAVNAAKKGFPVAIFSLEMSTLQMAQRIIAMEAEISSSA
                     LRVGKLSDDELKRFHHTLQTKSDLKIFVDDTPAINIFEMRAKCRRLKTQHNIQMIVID
                     YLQLMSAGSESKMGGNRTQEVSVISRALKGLAKELNVPVIALSQLSRALETRADKRPM
                     LSDLRESGSIEQDADIVGFIYRPDYHNLAEEGGINVAEPEAELLIQKHRNGALGNIKM
                     TFVREYARYQDPDDANHLFGFGSASAISDSGSSGIIRKGSRANDDDFIDAPF"
BASE COUNT         1226 a          930 c          995 g         1277 t
ORIGIN      
        1 tgatgtactt ggttctgcaa aaggctgggc cggcgcgttt ttgtataacg aaaaagccag
       61 gagtgccctg gagcgcttct ttgcccggga agataccctg tctgtaggcg tgtgcaacgg
      121 gtgccagtta ttcatcgaac tgggtcttat caacccggat cacgaagtaa agcctaaaat
      181 gcgtcacaat actagtgaga agcacgaatg tatatttacc accctgacca tcggtgataa
      241 taattccgtc atgttgtcat cccttgcagg atctaccctc ggtgtttggg tgtcacacgg
      301 agaaggtcga ttcagtttgc cgtatgaaga aaacaaatac catatcgtag ccaaatatgc
      361 ttatgacact tatccggcaa gccccaatgg ctcagattac aatacggcta tgatgtgtga
      421 tgtctcaggc cgccatctgg tcatgatgcc tcatatcgaa cgttccttct tccagtggaa
      481 ctggccttat tatcccgaag gacgtaatga tgaagtgtct ccgtgggtag aagccttcgt
      541 caatgccagg aaatggattg aagccagaga atgaagtaaa agtgaaaagg aatagatttt
      601 tatagggtta gttagccttc gaattttatc aaattattcg tatattcgat actttttcga
      661 tgctgatata ccgtttatga gaaaaattct gctctttgta tcctttgcac ttgctgtatt
      721 gatcggtatt cttacattca attatttttc catatcgtcc aggcagactg ccccaactca
      781 tgaaaaggcg gatatatctt ccatagtagg cgccgaagaa aggttgtcca gggcgatcac
      841 atttccgact gtctcatttg aagatagtac agatgcagac tatacgccat tcgttgcatt
      901 ccacaaatat ttacagtcaa cctttccggc aatatatgaa aatctggaag tcgaaaatat
      961 caatgaattt tcactgcttt ttcattggaa aggtagcgat agtaatctaa agccggtaat
     1021 actaatggct catcaggatg tcgttccggt gtcagaggat tcaaaaaacc tttggacagt
     1081 tcatccgttt gaaggtacca tcaaggatgg atttttatgg ggcagaggtt ctattgatga
     1141 caagtccaac ctaatgggcc agttagaagc agtcaatttc ttgttaggcc tgcaattcag
     1201 gcccaggagg gatatctacc ttgcctttgg ccatgacgag gaattgggtg gtgaaaatgg
     1261 cgccatgaaa atcgctcaac tgttgaaatc cagaggcatt gaagcagagt ttgtactgga
     1321 tgaaggcgga atcgtcactg acaatatggt gccgggtgta aggcaacctg ttgcccttat
     1381 aggtacttcg gaaaaaggtt atcttgactt aaccctttct gtccgcatcg aaggcggcca
     1441 ctcatccatg ccgaagaagg ataatgccat tggggtaata agcaaggcac ttaccaacct
     1501 gtcggatcat ccttttcctc ctcaattgtc accatcggta agggattttt tcagttatgt
     1561 aggcccgcac tcctcattta tcaacagact tgccatcagt aatatgtggt tattcaaacc
     1621 ggtgttgttc aagatgattg gaaagtcagc tcccggggat gccatgatcc ggaccaccat
     1681 ggtaccgacg attatccatg gtggtgacaa atcaaatgtt gtacccgata ttgctactgc
     1741 caatgtcaat cttcgtttgt tgcccggtac aaccattccg gatgccatag atcatgtccg
     1801 gaaggtcata aataatccgg ctgttaaaat agaggcgttg ggcgaacaaa aagaagccgg
     1861 tatcgtttct ccggcagaca atgatgcctt taaatatata tccgatgctg tgcataaaaa
     1921 ctttcctaat acactggttt cgccattcct gatgattgga ggcaccgatt caaagcattt
     1981 tgaaatactg tcgcaaaata tttataaatt tagtccactg atcgatccga ttggttttca
     2041 tggtgttgac gaaaggctca atctgaaagc ctatccccag gcaatcggat tttatgtcga
     2101 tctcatcaag tctttgtgaa agtacttaat acaataagag atcaatgcaa tgatccacat
     2161 ccggtcagtg attgaatgag atttgaatgg attggcatcc ggtattgatc ctgcggatct
     2221 tgatggccgc attttctgaa ttcttgactt tggtcaattt gattaagaag aggggagtaa
     2281 ataaaaatct aaaaaagata agcgccggtg tctctgaaga aaagaatacc tatatagatg
     2341 aatttccgga acccataaaa tcagtagcaa tgaccatcgc cgaacaatta tatcgcaaag
     2401 gagtaaagga agggaaagaa caaggtgttc aggaaggcct taaaaaaggc ttgaaacaag
     2461 gtattgaaaa agcgaagttt gaaataattg taaagttatt tgaaaatggt gcatctatcg
     2521 gttttataac caacattacc ggacttcccg aaaataaaat taaagaaatt ttgaatatac
     2581 aataatcggt ttgatggagc ttgattgtca tttatttatt caaatttcta catctccggc
     2641 agaaattttt tagattttta aaataaatca ttcggagggc tctattatta aaggtagctt
     2701 tatttatcaa agtcaaaatt taattcctcc cgaattggcc aatgcacctt cccggcaaac
     2761 aattccgtaa accttcccca taaaattgaa ctttagattg atgttccagc cttaattgac
     2821 tctctgattt ttccatcaaa tttttcgagc gatgcaaagt ttccggaacg gttctccctc
     2881 ttatggaaga ttatcagcaa attaaggatt tttaaccttg cgtcacccaa aacagttacc
     2941 cgactccatt cataatattg attattaggt ctttataaaa atttgtaaat taaaagtgcc
     3001 gaagtcagga aaagaatcgg atgataatgc cctgtaatag tttttgcagg gtattttttt
     3061 tcaggtcgga gtagaaggcg tatcaaattg ggaagcagca tacattggta aagatgaaaa
     3121 gggccttcta tcggtttatt tggcgaacgt ggcattctca atcaagtaca attaatgccg
     3181 attagcattt ttctcgagtc ggccggacaa gtatctgctc gcggaaggat ggagaatcgt
     3241 ggtagtcttg aaaaatgtaa ttaattttta aaacggagca tctataaaat catcatcatt
     3301 cgcccggctg ccttttctga tgatgcccga actgccggaa tcacttattg ctgaagccga
     3361 accaaatccg aataaatgat ttgcatcgtc cgggtcctgg tatcgggcgt attcacgtac
     3421 aaaggtcatc ttgatattgc caagtgcacc gttacggtgt ttctggatga ggagctctgc
     3481 ttccggttct gcgacattaa tgccaccttc ttctgcgaga ttgtgatagt cgggcctgta
     3541 tatgaatcct acgatatcag catcctgctc tatggagcct gattcccgaa ggtcggacag
     3601 catggggcgc ttatcagccc gcgtctccaa tgcccggctc agctgtgaca gggcgatgac
     3661 gggcacattc aattctttgg caagaccttt taaggcacgg gagatgacag agacttcctg
     3721 ggtgcggttg ccgcccatct ttgactcact cccggctgac atcaactgca ggtagtcgat
     3781 gacgatcatc tggatattgt gctgtgtctt cagccttctg cattttgccc tcatttcaaa
     3841 gatgttgatt gccggcgtat cgtccacaaa gatcttcagg tcggactttg tctgaagggt
     3901 atgatgaaaa cgctttaatt cgtcatcaga tagcttcccg actcgtaagg cggagctgga
     3961 tatttcagcc tccatggcta taatccgttg agccatctgc agggtggaca tctccaaaga
     4021 gaagatcgcc accggaaaac ctttcttggc tgcattgaca gccatggaaa gaacaagtgc
     4081 cgtcttaccc acactgggtc gagctgcgat gatgactaaa tcggaaggtt gccaccctga
     4141 agtaagcttg tccagctccc tgaaaccggt cgacactccg gtcaagcctt caggtcgact
     4201 ggccatctct tccattcgga gtcttacctg attggccata gcagtaaagg tctgtggccc
     4261 gttgttgagg ttgttgtcgg tgatcctgta taggttttgc tctgcctcat caaggatgtc
     4321 aaatacatct ttggtgtctt cataactatc ctgaatgatt tggttggaaa cattgattag
     4381 ttctcgctgg atatatttct ggaccacgat ccgggcatgg tgctcaat
//
LOCUS       DAOQQN010000281         4426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9222_length_4426_cov_5.681537, whole genome shotgun sequence.
ACCESSION   DAOQQN010000281 DAOQQN010000000
VERSION     DAOQQN010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9222_length_4426_cov_5.681537"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..593
                     /locus_tag="PLG24_10145"
     CDS             282..593
                     /locus_tag="PLG24_10145"
                     /inference="COORDINATES: protein motif:HMM:NF017470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HMZ25296.1"
                     /translation="METIKKWINIKGRASRTEFWGAMLFFITFMLVFGYIGELIEFRF
                     LPLYISPIGLIPFLATGMRRMQDVGKKGIFFFIPFYNFYIALTGGEKGPNQYGPNPRN
                     S"
     gene            619..1392
                     /locus_tag="PLG24_10150"
     CDS             619..1392
                     /locus_tag="PLG24_10150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ25297.1"
                     /translation="MNRPLSVVIIANNASRHIGEVIGAARRVSDDIIVLDSGSEDNTP
                     EIARDMGARVVFQDWLGYSATKNVGNSMAQHDMILSLDADEVLSEELIDHILIEDFNE
                     ETIYILDRANYYCGRRIRFCHWNPDRIPRIFDRRKARWQGEFVHEKLKFHTSYSQKLL
                     KGKLLHYSYDSYAQRLRKVKDYAILSARDRFRKGKRTTLPAMIVLPFFKFWITYIIHL
                     GILDGRAGLEIAWTDALGSWKRNRVLYDLQRNLTPECCK"
     gene            1752..2819
                     /locus_tag="PLG24_10155"
     CDS             1752..2819
                     /locus_tag="PLG24_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HMZ25298.1"
                     /translation="MDSAISAAHILASKHIPFCLYRLPGNEDKRIAIDSEVAGPHLIR
                     RFVVRPFSEESAAEPLIYTAMSRQNLNSDFIEYLKTCRNRIPISYLSFPLETSKSEYL
                     QGIRAYLDEIAKGVLKKAIYSRVIYADKPTGFNPIECFLRLCEIFPNAFVHLLSDPRK
                     GTWLGASPELLIKRQGNNVTTVALAGSQALQASGEYTWREKELEEHLLVGRHIEQVFA
                     DCDLRLLKKNGPFTIEAGTVAHLKTEYLFEATLEHNMESLIAKLHPTPAVGGTPVAEG
                     LACISRYEPYDRQYYCGIIGEVNSEEDISLYVNLRCMHVGENKIAIYAGGGITANSDP
                     EEEWLETILKSRTMADNINPR"
     gene            2831..>4426
                     /gene="menD"
                     /locus_tag="PLG24_10160"
     CDS             2831..>4426
                     /gene="menD"
                     /locus_tag="PLG24_10160"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HMZ25299.1"
                     /translation="MELSDKKGVRKIVLTLAKLGLQDVIISPGSRNAPFVISFLRHGG
                     FHCLSVRDERSAAFFALGKAIESHRPVAILCTSGSATLNFAPAISEAFYRRIPLIVLT
                     TDRPAEWTDQGDGQTIRQSHIYRNFIRHSYDLNGDADSYDQLWHIERCLCEGWNIATM
                     ADPGPVHFNIPLKEPLYITSEEEDDSNVRTFRELSLDRQLDHGSLKTMAAIFDNIKKV
                     MVLVGQHDVDHELSAGLTELAAHDNVVVLTESTSNVHHPHFVENIDRLITGLNDVDAA
                     ELMPDLLITIGDAVVSKRIKGLLRKYRPSHHWNIHRFDALMDTYQSLSLAIPLSGSGF
                     LNQFNQAVSGTSSDYNKRWQTLKERKKQLHDEFTVNSEYSDYSAFNRIVQAIPKDWVV
                     HVANSSPIRYVQLFDNSHIADMQCNRGTSGIEGCTGTAMGAASASPDKKFLLITGDIA
                     FHYDVNALWNEARIENLNIIVINNAGGGIFRIIEGPDKAAERLAFIETEMHLDAERIA
                     AHHDWNYLKALDNESLDTALKRLF"
BASE COUNT         1301 a          966 c          973 g         1186 t
ORIGIN      
        1 ggaatcaatc ccaaaacttt gacttccctt tttctaatgc gtaatctggg ttaatcacta
       61 gttatccggt agtaagcata gttttttcta taagatatat aatattgagg ctgtctttta
      121 aagctaaaaa tccggtttaa ttgttcctta attcactttg cattggagcc tgaaagaaaa
      181 cgagcatctc tccatgtatc caatcggctc aaaatctcct cattttatct ctttaatttg
      241 tacttttgca atccgttatt atcaattaaa atgaatgccg gatggagaca attaaaaaat
      301 ggatcaatat aaagggcagg gccagtcgaa ccgaattttg gggagctatg ctttttttta
      361 tcacatttat gttggtattt ggctatatag gtgagctcat cgaattccgg tttttacctc
      421 tatatatcag tcccattggc ctgatcccct ttttggctac aggaatgcga cgtatgcagg
      481 atgtcggcaa aaaaggcatt tttttcttta ttcctttcta taacttctac attgccctaa
      541 caggtggaga gaagggcccc aatcaatatg gtcctaatcc cagaaatagt tgagtaatct
      601 gaaatcaaca ttagagtttt gaaccgtccc ttgtctgtag tcatcatagc caataatgct
      661 tcacggcaca ttggcgaagt gattggagct gcccgcaggg tgagtgacga catcatcgtc
      721 ttggattcag gcagtgagga caatacaccc gaaatagccc gggatatggg tgccagagtc
      781 gtgtttcagg attggctggg ctatagcgca acaaaaaatg tgggaaacag tatggcgcaa
      841 cacgatatga tactttccct cgatgcagat gaagtattat cggaagaatt gatcgaccac
      901 atccttatcg aagatttcaa tgaagaaacc atctatattc tggacagagc caattactac
      961 tgtggtcgca ggattcgttt ctgccattgg aaccccgatc gtatcccccg aatatttgac
     1021 cgtcgaaaag caagatggca gggcgaattt gtccatgaga aactgaaatt tcatacttcc
     1081 tattcccaaa agttactgaa aggcaaattg ctccattatt cgtatgattc atatgcacaa
     1141 cggcttagaa aggtgaagga ttatgcaata ctttcagcaa gggaccgctt tcgtaagggt
     1201 aaaagaacta ctcttcccgc aatgattgtc cttccgtttt tcaaattctg gattacatac
     1261 atcattcatt taggtattct ggatggtcgg gccggccttg agattgcatg gaccgatgcc
     1321 ctcggatcct ggaaaagaaa tagagtgctg tatgacttgc aaagaaatct tacgcctgaa
     1381 tgttgtaaat aacagtcgga tttatttaaa atcttaatct aaacagtagc tctgctgccg
     1441 tatttaagac ggataacgga tctcaagacc gtacgctgaa ccggattgtt taaaggatat
     1501 ttttataaac cacaaaaggg acaactcttt tcctgacttc cgcacttttt acaactaaaa
     1561 cactgatgta caagtagttg cattttcaat taggcactac aataaattta taatcaattg
     1621 acattcacat agttggtgtg tttattgcag gaaagcactt gtatgctaga gtctgcatgt
     1681 cattttgaag aagtgaatag ttacaaatcg catattcttg ctacttttgc caatcaaatc
     1741 tttgagaata ggtggactca gccatttctg cagcgcacat cctggcgtca aaacatattc
     1801 ctttttgtct ttaccgttta cccggcaatg aagacaaacg catcgccatt gattcggagg
     1861 tggcaggccc tcaccttatc agaagatttg tcgtacggcc attcagtgaa gaatcagcgg
     1921 ctgaaccatt gatatataca gcaatgagcc ggcagaatct caattcagac tttattgaat
     1981 atcttaagac ctgtcgcaat cgcattccta tctcctatct cagtttcccc ctggagactt
     2041 ccaaatcaga atacctccag gggataagag cctatctcga cgaaatcgcc aaaggtgtac
     2101 tcaagaaagc catttattca agggtcattt acgcagacaa gcctacagga tttaatccca
     2161 tcgagtgttt cctccgtctt tgtgaaattt ttccaaatgc tttcgtccat ttactatccg
     2221 atccacggaa gggcacctgg ttgggtgcaa gtcccgaatt acttataaag cgacaaggga
     2281 ataatgtcac gactgtagca ctggcaggga gtcaggcact tcaagcgtcg ggtgaataca
     2341 cctggagaga aaaagaactg gaagaacatc tgctggtagg aaggcatatc gagcaggtct
     2401 ttgcagattg tgaccttagg ttattgaaga agaatggacc atttaccatc gaggccggta
     2461 cagtcgccca cctcaaaaca gaatatctct tcgaggcaac cctggaacat aatatggaaa
     2521 gcctgatagc aaagctgcat ccaacacctg ccgtcggtgg gactcctgtt gcggaaggac
     2581 ttgcgtgcat cagcaggtat gaaccttatg accgccaata ctattgcggc atcataggag
     2641 aagtgaacag tgaagaagac attagcctct atgtcaatct acgctgtatg cacgtcggag
     2701 aaaacaagat cgcaatatat gcgggaggag gcatcactgc caattctgac ccggaggaag
     2761 aatggctgga gacgatactt aagagcagaa ccatggctga caacatcaat ccccgttaaa
     2821 acaaaaagaa atggaacttt cagataaaaa aggtgtccgt aagattgtac taaccttggc
     2881 gaagttgggg ttacaggacg taatcatcag cccggggtca cgcaatgcac ctttcgtgat
     2941 ttccttcctt aggcatggtg gcttccactg tctcagtgta agggatgagc gcagtgcagc
     3001 attctttgcc ctaggtaagg cgatcgaatc ccaccgacca gtggctattc tatgtaccag
     3061 tggatcggcg accctaaatt ttgcaccggc tatatccgaa gcattttacc gacggattcc
     3121 actcatcgtt cttactaccg acagacctgc agaatggacc gatcaaggag acgggcaaac
     3181 tatccgccaa agccacatct acagaaattt tatacgccat agttatgatc taaatggtga
     3241 cgccgattca tacgatcaac tgtggcacat tgaacgctgc ctatgtgagg gttggaatat
     3301 agccacaatg gcggatcccg gccccgttca cttcaatata cccttgaagg aaccgctata
     3361 tataacttcg gaggaggagg acgactccaa tgtgcgtaca ttccgagaat tgagtctcga
     3421 cagacagttg gaccacggaa gtttgaagac gatggcagca atatttgaca atataaagaa
     3481 ggtaatggta ttggtaggtc aacatgatgt ggatcacgaa ttatcagcgg gcttaacaga
     3541 gcttgccgct catgacaatg ttgtagtatt gacagaatcc acttccaatg tacaccatcc
     3601 tcattttgtc gagaacatcg accggctcat cacagggttg aatgatgtag acgcagcaga
     3661 attaatgccg gacctgttga tcacaattgg agatgctgtt gtatctaaac ggataaaggg
     3721 cctcctccgc aaatatcgtc cctctcatca ctggaatata catcgctttg acgcactaat
     3781 ggacacctac caatcgctct cactcgccat tcccctttcc ggttctggct ttttgaatca
     3841 gttcaatcaa gcggtatcag ggacgtcatc ggattacaat aagcgatggc agacattaaa
     3901 agaaagaaaa aagcaactgc acgatgaatt tacagtaaat agcgaataca gtgattattc
     3961 ggcttttaac cgaattgtac aggcaattcc aaaagattgg gtagtgcatg ttgccaacag
     4021 ttcaccgatt agatatgtac aattgtttga caatagccac atcgcagaca tgcagtgtaa
     4081 tcgcggtacc agcggcatcg aaggatgtac gggaacagca atgggagcgg cttctgcctc
     4141 acccgataaa aaattcctgc ttatcaccgg tgacattgca tttcattatg acgtcaatgc
     4201 cctgtggaat gaagctagga tagaaaatct aaatattata gtcatcaaca acgcaggcgg
     4261 aggcatcttc cgcatcattg aaggcccgga caaggcggcc gaaaggttgg cgttcattga
     4321 gactgaaatg catttggatg ccgaacgtat tgcggcacat catgactgga attacctaaa
     4381 ggcgctcgat aacgaaagcc ttgatacggc actaaaaagg ttattt
//
LOCUS       DAOQQN010000282         4411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9264_length_4411_cov_4.943526, whole genome shotgun sequence.
ACCESSION   DAOQQN010000282 DAOQQN010000000
VERSION     DAOQQN010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9264_length_4411_cov_4.943526"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PLG24_10165"
     CDS             <1..375
                     /locus_tag="PLG24_10165"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HMZ25300.1"
                     /translation="VPIGAVIVARNQVIARAYNQVQMLNDVTAHAEILAITAAEQAYG
                     SKYLNDCTLYVTVEPCPMCAGAIKWSQLDRLVYGTDDEKYGFMKFGKSILHPATKVAY
                     GVCREEAMELMQQFFAERRKHK"
     gene            480..1070
                     /locus_tag="PLG24_10170"
     CDS             480..1070
                     /locus_tag="PLG24_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25301.1"
                     /translation="MIRIERYIGSVIIGLVFSILLFGVFVVGMSSCGTSGARLRPFTE
                     RFYKDSGLTYDQLKHVQFYIDRDIILYRMIGSNQSTVDQGRIATRGGRKVEEIVIRSG
                     TPGTLVFMPKENRLGISFDADNAGNYLMFGPNPKNNNRYCLLAIEWDRDEGMVSYGPN
                     HWKTPASSAFATLMVNHRNMKKTKYSSQSPEGRVVH"
     gene            complement(1093..2403)
                     /locus_tag="PLG24_10175"
     CDS             complement(1093..2403)
                     /locus_tag="PLG24_10175"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HMZ25302.1"
                     /translation="MTDNIKAQLKSNTFYIIKMALKFKSLKQIGYIFFLIIPSLLLLS
                     CSKETQVVQEPTRDIDIKIPHIHIKIGDSSEIDSKDYKAAQIFIDSKGGFKDIQVSGK
                     VAGRGNYSWSLPKKPYKIKFDNAQSIFGLPAYKKWVLLAEYMDGSMLYNSIPFGTAYL
                     LKLPYSNHIFPVDVTINGQYRGLYILTESLEVGKGRIDIGEDGTLLELDSYYDEKWKF
                     ISGKFKLPVMVKYPEEEDMTEEKLTEIKGDFEALEKLIYATDFPNNNYLDLFHDTAFV
                     DYMIVYQLSANFEINHPKSTYMNKKSNGKYKMGVIWDFDWAMGYNDITGIHYDPAYAT
                     SPLIYENGNNPGTKFFGRIMQDPKIQKLFLHRWKWFRQHKYQSLRKFVSDYSLLINES
                     YKEDHNLWGLRGSTGVLASDLQRALSWLDARADYIDDYADSFNQ"
     gene            complement(2794..3462)
                     /locus_tag="PLG24_10180"
     CDS             complement(2794..3462)
                     /locus_tag="PLG24_10180"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HMZ25303.1"
                     /translation="MYSKTTLIDDYRHKGLRKKLLQELRSKGITSDTVLTAFDKVPRH
                     FFLDKAFEELAYQDRPVPIGYRQTISQPYTVALQSQLLNVQKRQKILEIGTGSGFQAC
                     ILAEMGARVYTIERQEGLYRKTMSKLEQIGYSHIKCFLNDGFKGLPYHAPFDKIIVTA
                     APGELPYDLIDQLVIGGVMVLPKGNHKNQKMMLLTKTSHNTYTLEEKVSAYFVPMLEG
                     IEQL"
     gene            complement(3548..4129)
                     /locus_tag="PLG24_10185"
     CDS             complement(3548..4129)
                     /locus_tag="PLG24_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ25304.1"
                     /translation="MQLQSICDQELIQLYLNGNERAFEELLHRHKSKIYTTIYMFVKD
                     EHQAEDIFQEVFIKIIDTLRRGKYNHEGKFVQWAVRIAYNLCVDNFRRSKRRSIFNST
                     ENFDIFDVLSVAEDSHEQAIIKSQSHERLRKLIDDLPPEQREVIILRHYADMSFKEIS
                     QVTRVSINTALGRMRYALINIRRNLTEKEALAI"
BASE COUNT         1220 a          856 c          944 g         1391 t
ORIGIN      
        1 gtacctattg gagctgtcat tgtggcacgc aatcaggtga ttgcccgggc atataatcag
       61 gtacagatgc tcaatgacgt cacagcgcat gccgagatcc ttgccatcac tgctgcggag
      121 caggcttatg gctcaaaata ccttaacgac tgtacactct atgtcactgt cgaaccctgt
      181 cctatgtgtg caggagcgat taaatggtca caattagaca ggttggtcta tggtacggat
      241 gacgaaaaat atggatttat gaaattcggg aagtctattc tccatccggc tactaaagtt
      301 gcatatggtg tctgcaggga agaagccatg gaattgatgc agcaattttt cgctgaacgc
      361 cgtaagcaca aatagttaaa attgatttaa agcatggtta aacaatgtta aaaaacgcct
      421 tttgaccttt gtccaggcgt tatcttttga cagtaaaagg taaaaatgat attgtgatta
      481 tgataagaat agaaaggtac ataggtagtg tgattattgg gctggtcttt tcgatacttt
      541 tatttggagt tttcgtagtg ggtatgtctt cctgtggtac atcaggtgca cgattaaggc
      601 cttttaccga gcgattttac aaagactcgg gactcactta tgatcagttg aagcatgtcc
      661 agttttacat tgatcgggat atcattctgt acaggatgat tggtagcaat caaagcacgg
      721 ttgatcaagg caggattgcc acccgtggtg gtcggaaagt ggaggaaata gtaataagga
      781 gcggtacacc ggggacactt gtattcatgc ccaaggagaa cagacttggc atttccttcg
      841 atgcggacaa tgccggtaat tatctgatgt tcggtcctaa tcccaagaat aacaatcgct
      901 attgtctgct tgccattgag tgggataggg atgaaggaat ggtgtcgtac gggccgaatc
      961 attggaaaac tccggcttcg agtgcctttg ccactttgat ggtcaatcac cggaacatga
     1021 agaaaacgaa atactcctca cagagccctg aaggtagggt ggtacattaa agtattcaac
     1081 actatattct tcctattgat tgaaagaatc cgcatagtca tcaatatagt ctgctcttgc
     1141 atccagccag cttaaagcac gttggaggtc cgaagctaag acacctgtag aaccccttaa
     1201 tccccaaaga ttgtgatctt ccttatagga ctcgttgatt agcagggaat aatcggacac
     1261 aaattttctg agggactggt atttgtgctg cctgaaccat ttccacctgt gaaggaatag
     1321 tttttggatc ttgggatcct gcatgattcg cccaaagaat ttagttcctg ggttgtttcc
     1381 attttcatat atcaatggag aggtagcata agccggatcg taatggattc ctgtaatatc
     1441 attgtacccc attgcccagt caaaatccca gataacgccc attttgtatt tgccattgga
     1501 ttttttgttc atgtatgtac tcttggggtg gttgatctcg aaattggcag aaagctggta
     1561 cacaatcata taatcaacaa aagccgtgtc atggaacaaa tccagataat tgttgtttgg
     1621 aaagtccgtt gcatagatca gtttttcaag cgcttcgaaa tcgcccttga tttcggtcag
     1681 cttttcttcc gtcatatcct cttcttcggg atacttaacc atgacgggaa gtttgaattt
     1741 gcccgaaatg aatttccatt tttcatcata atacgaatcc agctcgagta aggttccgtc
     1801 ctctccgata tcaatgcggc ccttgccgac ctccagactt tcggtcagta tgtacagacc
     1861 gcgatattgg ccattgatgg tgacatcgac aggaaaaata tgattggaat atggaagttt
     1921 caaaaggtag gcagtaccaa atggaatgga gttgtataac atggaaccgt ccatatattc
     1981 agccagcagg acccactttt tataagcggg aaggccgaaa atggattgag cgttgtcaaa
     2041 ttttatttta tagggcttct tcgggagcga ccaactgtaa ttgcctcttc cggcgacttt
     2101 gccacttacc tgaatatcct tgaatccacc tttgctgtcg atgaatattt gcgctgcttt
     2161 gtaatctttg gaatctattt ctgaactgtc accaatttta atatgaatat gtggtatctt
     2221 tatgtctata tcgcgggtcg gttcctgtac tacctgtgtt tccttactac atgacaaaag
     2281 taagagcgaa ggaatgatca aaaaaaaaat ataaccgatt tgtttcaaag acttaaactt
     2341 aagcgccatc ttaataatat agaatgtgtt tgacttgagc tgagctttta tgttatctgt
     2401 caacttagcc cgttattacc cctcaaatgt aagaaataac tatcttttca ggtaatataa
     2461 tagtccgggt tgatggcggc ttgggtaaat aaatcattta gatgaatata attgtacctg
     2521 gcatcacggg cattcgccta atcctctcgt catggttgtc cggttagacg taatggtttg
     2581 ggaggttgtc agtcctgccc aatgattgag cgtgtacgaa aagttgtacg attggacaag
     2641 aaatttatca taggatttat ggacacagaa aagatcatac ctgctgtaaa tttgtcggta
     2701 attcaattgt gaatgttctc ttgacctatt atagcgctcc tgtcttgatg atccaacatg
     2761 gaaatatcat cgcctgtcct cttgtacaaa tccctatagt tgttcaattc cttcgagcat
     2821 agggacgaag taagcactca ccttttcctc aagcgtatag gtattgtgtg atgttttggt
     2881 tagcaacatc attttttgat tcttgtgatt tccttttggc aataccatca caccaccaat
     2941 gaccaattga tctatcaaat catagggcaa ctctcccgga gctgccgtta caatgatttt
     3001 gtcaaaggga gcatggtatg gcagcccctt gaatccatcg ttgagaaaac atttaatatg
     3061 ggagtatcct atttgttcca gtttgctcat tgtcttacga taaaggcctt cctgccgttc
     3121 gattgtatat acacgggcac ccatttctgc cagaatgcag gcctgaaagc cggatccggt
     3181 tccgatttca aggattttct gccttttctg aacattgagc aattgactct gaagggctac
     3241 cgtgtatggt tgagaaatgg tctggcgata tccgataggg acaggccggt cctggtacgc
     3301 cagttcttca aaagctttgt ccagaaagaa atgtctgggc accttgtcga aggcggttag
     3361 gacggtatcg ctggttatgc ctttacttct gagctcctgc agcaattttt ttctgaggcc
     3421 tttatgcctg taatcgtcga taagtgtagt tttcgaatac atattaacgc aaagttacat
     3481 atgtaaaatg aaaaaagcta acttattgta aaataaatta gctttttttt gaaataaagg
     3541 tattgactta gatcgccagg gcctctttct cggtcagatt gcgacggatg ttgatcaggg
     3601 cataacgcat cctgcctaaa gcggtattga tgcttaccct tgtcacctgg cttatttcct
     3661 tgaaactcat gtctgcgtaa tggcgcagga tgatgacttc tctttgctcc ggaggcagat
     3721 cgtcaataag cttgcggagt ctttcatgac tttgactctt gatgatggcc tgttcatggc
     3781 tatcttcagc taccgacaaa acatcgaata tatcgaaatt ttcggttgag ttgaagatag
     3841 agcgtctttt tgatcttctg aaattatcca cacaaaggtt gtaagcgatg cgaacggccc
     3901 attgtacaaa tttaccttca tgattatatt tccctctacg caaggtatca ataatcttta
     3961 tgaagacttc ctgaaagata tcttcggcct gatgctcatc tttgacaaac atgtagatag
     4021 tagtataaat cttgctcttg tgcctgtgaa gcaattcttc aaatgcacgc tcatttccgt
     4081 tgagatacag ttggattagt tcctgatcgc agattgattg taattgcata atttctactt
     4141 tttttactta ggataatgga ttgttgaaaa gtaaaaacta tgcgtatagg ctggttaaat
     4201 atttttaaac taaaaatgtc ttttgaataa taatttcagt tcaaagatat attttatttt
     4261 ttactcatac tctccttaac acaaatttaa cttaagataa attgctttgg atcttcagaa
     4321 gtcagtgttt tacacaggtt ctaaacatgg attaaatgtg ttttttttta tttttatacg
     4381 aaagttaaaa aataaataga ccaaaatttg g
//
LOCUS       DAOQQN010000283         4385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9345_length_4385_cov_5.605312, whole genome shotgun sequence.
ACCESSION   DAOQQN010000283 DAOQQN010000000
VERSION     DAOQQN010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9345_length_4385_cov_5.605312"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLG24_10190"
     CDS             <1..374
                     /locus_tag="PLG24_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25305.1"
                     /translation="LSIPELNFDTITYSNILFVDGQEKTLEASLPLTRTFFGINWSDD
                     NKIGVRLGTYPILANKGYIAIKNKVDFDFYKAQSGSVTITHSPEGEGLPLRGIFTLYV
                     TTDVKKTPIKVVGSFKATYKS"
     gene            427..960
                     /locus_tag="PLG24_10195"
     CDS             427..960
                     /locus_tag="PLG24_10195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ25306.1"
                     /translation="MNEHIIIQNCLRNDRKSQKMLYELYYQLMMAVCLRYCKDEYMAQ
                     EALQNGFIRVFSGLHTYDSNKPFGPWIRKIMINCCLDQLSRKSVMANLDDIMPTESTT
                     IHDPEFEITDAGLTRLIESMPIGYRTVFSMYVIDEMSHLEIAEALQISEATSRSQLFK
                     ARNYLKAALTNKRKLFL"
     gene            957..2342
                     /locus_tag="PLG24_10200"
     CDS             957..2342
                     /locus_tag="PLG24_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25307.1"
                     /translation="MTNKKSPIEDRIAGLYRQQKAPVPESIWQNIEISLNAKQKKRRR
                     SIWLFISMMTLFMILGTLAVLPLLRKTHKADIADRDSAFEYRHDKIANDASEITDTSS
                     QSTEQAIASSRRISMPETILKTDGTTSLIRNQRSSAKPVESNMTVKVTSQSNERNTTP
                     SDKIAAVDESATRYPISAFDGGVHIERSVPQQIPGQLTLMEGRDIYPTWIGPNREMMS
                     LPWQNQHEKNAGIIRQHRLRPFIGLSAGIGLPFRKMVNHSVESELYIGRQGTEHPLYL
                     TNIQADAGLLLDDSWSIKSGIEWQQAVEQFSYKKFDATRLEYKTDDITGRIDDTSLVR
                     GLLTEQFGNKYTMLNIPLYLGYEKRFGRWVFGIDGGVGFNIHFRTSGKVLVDDQKIQS
                     LDNRHDIYASSTGISWRVGASCLRSIAPGTMVYARLMCMAYPDSWTLSTHPNEIRYRF
                     LTANVGLRKVF"
     gene            complement(2873..3343)
                     /locus_tag="PLG24_10205"
     CDS             complement(2873..3343)
                     /locus_tag="PLG24_10205"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HMZ25308.1"
                     /translation="MALEIERKFLVDHDAWEKVDRPVGVPFRQGYIVKDQQKVVRVRI
                     TPDSGFITIKGESKGAVRSEFEYPIPREDANELLELFCSYEVAKRRYFMSFRAKTWEV
                     DVFEGKNSGLIVAEIELNSADELFEKPDWVGEEVTEDPRYYNAFLAEHPYAEWM"
     gene            3476..4051
                     /locus_tag="PLG24_10210"
     CDS             3476..4051
                     /locus_tag="PLG24_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ25309.1"
                     /translation="MEILIRRACPEDNEHIVAHIILAMEDIIFRFIGEESSEKAFAFL
                     SSLVKERGNLYSYENCWVAEHGSEVVATAVVYDGADYSRLKIPVANRVKAMFGRSFEF
                     ENETGPGEYYVDSVGVSREHQGKGIGSRLFRFLIEEYVSKKRTALGLLVDKENPTAKK
                     LYLKLGFEKVGDLVLAGKSMEHLQVKHKNIY"
BASE COUNT         1347 a          888 c          961 g         1189 t
ORIGIN      
        1 cattatcaat tcctgaatta aactttgaca cgatcaccta ttccaatata ctttttgtcg
       61 atggacagga aaagaccctc gaggcctctc ttccattgac acgtactttt ttcgggatca
      121 actggagcga cgacaacaag atcggcgtgc ggctcggaac ctatccaatc cttgccaata
      181 aagggtatat cgctataaag aacaaggtag acttcgactt ttacaaggcg caatccggat
      241 ccgtgacaat cactcacagc ccggagggag agggtttgcc gctcaggggt atttttaccc
      301 tatatgtgac aaccgatgta aagaaaactc cgataaaagt tgtcggaagc tttaaggcaa
      361 cctataaatc ctgattaatt atcgaacaat tcaccattaa gaaaatatgt gatattgaga
      421 taaagcttga atgaacatat aatcatacag aactgccttc gcaacgaccg taagagtcag
      481 aagatgttat atgagttgta ctaccaactc atgatggcgg tttgtttgcg ttattgcaag
      541 gatgagtata tggcacagga ggctttacaa aatggtttta ttcgggtatt ttctggatta
      601 cacacctatg attccaacaa gccgtttggc ccctggatca ggaagattat gatcaattgc
      661 tgtctggatc agttgtccag aaagtcagtg atggccaatc tggatgatat catgcccaca
      721 gagagcacaa cgattcatga tccggaattt gaaattacag acgcaggatt gacacgactg
      781 attgaaagta tgccgattgg ctaccgtact gtattttcaa tgtacgtcat tgacgaaatg
      841 tcacatctgg aaatagcaga agcacttcaa ataagtgagg ctacatcaag gtctcaacta
      901 ttcaaggcaa ggaattattt gaaggccgcc ctaaccaata aaagaaaatt attcctatga
      961 caaataaaaa aagcccgatc gaagacagaa ttgccggatt gtaccgacaa caaaaggctc
     1021 cggtaccgga aagtatctgg caaaatattg aaatcagtct gaatgccaaa caaaaaaaac
     1081 gtcgtcgttc catatggctt ttcatttcca tgatgaccct cttcatgata ttggggaccc
     1141 tggcagtctt gccgctatta cgcaagaccc acaaagccga tattgccgat cgagactcgg
     1201 ccttcgaata tagacatgat aaaattgcca atgatgccag tgagataaca gacaccagct
     1261 cccaatccac tgaacaggca atagccagtt cgaggaggat ctccatgccg gagacaatat
     1321 tgaaaaccga cggtacaact tccctgatca gaaatcaaag gagcagtgca aagccggttg
     1381 agagcaatat gacggtaaaa gtaacaagtc aatccaatga acgaaataca accccatcgg
     1441 ataagattgc tgctgtcgat gaaagcgcca caagatatcc aattagtgct tttgatggag
     1501 gcgtccatat tgaaagatca gtgccccaac agatcccggg acaattgacc ctcatggaag
     1561 gtcgtgacat ttatccaacc tggattggac cgaaccggga aatgatgagc cttccatggc
     1621 aaaatcagca tgagaaaaat gccggaataa ttcgtcaaca ccgccttaga cccttcattg
     1681 gtttgagcgc aggtatagga cttccattta gaaaaatggt caatcactct gtagaaagcg
     1741 aactgtatat tggaagacaa ggtacggagc atccgcttta tctgacgaat attcaagcag
     1801 atgcagggct attgctggac gacagctgga gtatcaaatc aggtatcgaa tggcagcagg
     1861 ctgtggaaca attcagctac aaaaaatttg atgctacccg cctggaatat aaaacagatg
     1921 atataacggg gcggatagat gatacttcac tggtaagggg cctattgaca gaacaatttg
     1981 gaaataagta cacaatgctc aatattccat tgtacctggg gtatgaaaag cgttttggca
     2041 ggtgggtgtt cgggattgat ggcggtgtcg gttttaatat acatttcaga acttcgggaa
     2101 aagttcttgt tgatgatcaa aaaattcagt cactggacaa caggcatgat atttatgcct
     2161 catctacagg catcagctgg agagtaggtg ccagctgtct gagaagcatt gctcccggaa
     2221 cgatggtgta tgcccgcctg atgtgtatgg cctatcctga tagttggacc ctaagcaccc
     2281 atccaaacga gattcgatat agatttctaa ccgccaatgt tggcctgaga aaagtttttt
     2341 gattagtgaa agggctttcg gtaatcaaca gcgacctccg accagacatg attggccact
     2401 attaaggttt cgttcttgtc agatatacat tccagaaagg gatcaatcgt aattcctgtt
     2461 tagttgtgat ttcctcattg gaggatttga ctgtccgggt aaatattctt gaaagcttcg
     2521 ggtaaatgta caggaactgt tgccggagat attttctgtt attacctgaa tggatttgcc
     2581 ggactgaatt caaattaact acgttcatcc tttatacatt acccatctcc ataaataaat
     2641 ctgtcatgac gaaagcttcc tctttgggtt ttcagcgcac tgcacggcct ttcaacagta
     2701 gatgtccaaa cgataatcgt agccctggca ttgtgattga actgtagttg aaaaacattt
     2761 cgaaaggagg gtgccaatat ctgaggccat gtccaactta cggatgcaaa acaggggtca
     2821 ggaaggataa gcagaaaatg accctgacat ttacctgcaa aaaaatgaat ggttacatcc
     2881 actcagcata gggatgctcg gccaggaatg cattgtaata tctgggatct tccgtaactt
     2941 cttcgcccac ccaatccggc ttttcgaaca attcatctgc tgaattcaat tctatctctg
     3001 ccacgatgag accagaattt tttccttcaa agacatcgac ttcccatgtc ttagcccgaa
     3061 atgacataaa atatcgtctt tttgcaacct cgtatgaaca aaataattcc agtagttcat
     3121 tggcatcttc cctgggaatg ggatattcaa actcgcttct cactgcaccc tttgactcac
     3181 ccttgatcgt aataaagcca ctatccggag tgattctgac tcgaacgacc ttttgttgat
     3241 ccttgactat atagccttgt cggaaaggga caccaacagg tctatcgact ttctcccatg
     3301 cgtcgtgatc aacgagaaat ttcctttcaa tttccaatgc catttttgat atgatgtttg
     3361 atttcaataa aaagttgtat gacaagatac acatttgtga caaagaaaag cgataactga
     3421 tcgaaagagt tatattgatt accttcacct tcggaacaat cttatttacc taaaaatgga
     3481 aatactgata agaagggcct gccctgaaga taacgaacat attgtcgccc atatcatcct
     3541 ggcgatggaa gatatcatct ttcgctttat cggagaagag tcatcggaga aggcatttgc
     3601 gtttttaagc agtctggtca aagagcgagg caacctgtat tcctatgaaa actgctgggt
     3661 tgctgagcat ggaagcgaag ttgtcgccac ggcagttgta tatgatggtg ctgattatag
     3721 caggttgaaa attccggtgg cgaatagggt aaaagccatg tttggtcgtt cttttgaatt
     3781 tgagaacgaa accggcccgg gagaatatta cgttgattcg gtgggagtca gccgggaaca
     3841 tcagggcaag ggaataggct caaggttatt cagattcctg atcgaagaat atgtttccaa
     3901 aaagagaact gcactgggtt tattggtaga taaagagaac ccaactgcaa agaagcttta
     3961 tctgaaactc ggatttgaaa aagttggtga tcttgtgctt gccggcaaat ccatggaaca
     4021 tttgcaggtc aaacataaga atatttatta agtgtccgga attagtttgg atccggtgtt
     4081 ttcattgaaa aattagctaa agttggtagt ttgctacaat cagacagaca tttattcaaa
     4141 attcgataga agaatttaat ttactgatat tggatttcat tgagcagtct gcatttgatt
     4201 aaagctacac cgacaaaagt tcatctttgc tttcactata caatcttgct cagaaggtct
     4261 attgacagat gaagacaatg taaggcattg atctgttgat tgatttcccg gttgaaagcg
     4321 ggattgcccg ggctttgttt cttatacgaa agttaaaaaa taaatagacc aaaatttggt
     4381 gtatt
//
LOCUS       DAOQQN010000284         4383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9351_length_4383_cov_5.135628, whole genome shotgun sequence.
ACCESSION   DAOQQN010000284 DAOQQN010000000
VERSION     DAOQQN010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9351_length_4383_cov_5.135628"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLG24_10215"
     CDS             <1..199
                     /locus_tag="PLG24_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HMZ25310.1"
                     /translation="QLGRATSVDTIKAGLKAGDVILKVADFVITDIQTYMKALNGLEP
                     GSKVPVEFLRDGKNMTLDVQF"
     gene            205..1044
                     /locus_tag="PLG24_10220"
     CDS             205..1044
                     /locus_tag="PLG24_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25311.1"
                     /translation="MGRIVIFTVFITISLCSCDNSKFNVDIPTKNFDGLWLVSSVTGP
                     KKNDAALPAMIKFNTGRWIQFNDDSTYTTNIKGQFDYGKFSPLGNGKDSVEMRSFRGD
                     IYHISARYHPNGTGEILHRITIPDLSGTFDYYFYCNVRKYSFRHDTFNPYSLPNNQWR
                     LPAEHSENDSLLVKRMVNHIDFWIAYLNMADKLELKSFNYSNVNTCFKFSPYGIIMEN
                     FKNWESTFQTLFYTKKEAERAYKLTSEAIYKSKYVKHENAYIQGINLFKQIQFNLLNT
                     NKG"
     gene            1283..3271
                     /locus_tag="PLG24_10225"
     CDS             1283..3271
                     /locus_tag="PLG24_10225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ25312.1"
                     /translation="MNTHNTLIFVCLYFCTNLLTAQPIPSFPGAEGFGAQATGGRGGR
                     VIYVTNLNANGPGSLNAALSESGPRYILFKVSGVIDAAAELREGDVTIAGQTSPGGII
                     TRGFIVDQVYETGGSGDNIIMRHIRSRPHDPAIVPSDHYVLDDALRLDGASNMIVDHC
                     SFANARDECIQISQSSDITIQHSMLSETVGPHYIYGGMLINYSTLEHPQDNLSIHHNT
                     WNRIAGRLPEVICESPYCNSGPLHLELSNNLLWDPQNYVWYGANIDPSGEDYSFFVHM
                     NWVNNFMKTRTTFPYAMMEMRFLQYAQNQIYGLGNNMDIYPMYSDYQLFYCCNDFNQP
                     GNNPNNDPGIAQLLSNRHNFPSITYDPSNNLVDYMIANCGAFPRDPMDTRLMNPLVQN
                     IIEPLPVDGVDYFNDAFITNAPTTPPDDTDMDGMPDYWELGHGLNPSIQDHNGTQLSL
                     SITGVSGYTNLECYLNCLSDGLVNGQSTPSCGIALPVILKNFEVKKYDQTVILMWKVT
                     EERNFSGYSIERSKDGKVWTTIGYVEASTKENANANEVTYDYMDDQPMPGVNYYRIKM
                     SDKDGSIQYSLVKQATFQNHAEIDIYPNPTTGIIYFNGLKGKADVQIADQIGKIHIST
                     TIHNTSNKLDVSNLINGMYFIKVTTEYGNTITKKVMKN"
     gene            complement(3335..3409)
                     /locus_tag="PLG24_10230"
     tRNA            complement(3335..3409)
                     /locus_tag="PLG24_10230"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3372..3374),aa:Val,seq:gac)
     gene            complement(3464..3658)
                     /locus_tag="PLG24_10235"
     CDS             complement(3464..3658)
                     /locus_tag="PLG24_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25313.1"
                     /translation="MKKLILSLIVVGSVSFAMTSCKSETTTTEPETTTVDTTAAPAPE
                     EAAPAADTTAAPAADTTMQK"
     gene            complement(3881..>4383)
                     /locus_tag="PLG24_10240"
     CDS             complement(3881..>4383)
                     /locus_tag="PLG24_10240"
                     /inference="COORDINATES: protein motif:HMM:NF019122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol 3-phosphate dehydrogenase"
                     /protein_id="HMZ25314.1"
                     /translation="GPDFAVFGSPELLGAELAGSLKNVIALCTGMLNGIGMGKNLEAL
                     LITRGLRELIYIGKAMDVPARAFLGTAGIGDLVATATSPSSRNYTFGRRLAMGETKEQ
                     IAASMDGLAEGVRVLKITNGLIKHYKVNAPIITLLHKVVFENYNLQTAIRFLMRYPYV
                     QDVDFL"
BASE COUNT         1409 a          870 c          833 g         1271 t
ORIGIN      
        1 gcaactcgga cgggccactt ccgttgacac catcaaggcc ggactcaaag ccggcgatgt
       61 tatattgaaa gtggctgact ttgtcatcac tgatattcaa acctatatga aggctttgaa
      121 cggacttgaa cccggatcaa aagtgcctgt tgaatttttg cgtgatggca aaaacatgac
      181 tctggatgtc caattctgac aaggatgggt cggattgtaa tatttacggt tttcataaca
      241 ataagcttgt gtagttgtga taacagcaag tttaatgtag atatacctac aaaaaatttt
      301 gatggtttgt ggctggtatc ctcggtaacc ggcccaaaaa agaacgatgc tgcactgcct
      361 gcaatgatta agttcaatac aggtcggtgg atacaattca atgatgacag cacctataca
      421 accaatatca aaggtcagtt tgattatggt aaattctctc cattgggaaa tggaaaagat
      481 tccgtcgaaa tgcgcagctt cagaggagac atatatcata tatcggccag ataccatcca
      541 aatggtacag gtgaaatatt gcacaggatt actatacctg atctatccgg gacatttgat
      601 tactattttt attgcaatgt ccgtaaatat tctttccgcc acgatacatt caatccatat
      661 tccctgccga acaatcaatg gcggcttcct gcagaacaca gtgagaatga ttccttacta
      721 gtcaaaagga tggttaatca tattgacttt tggattgctt acctcaatat ggcagataaa
      781 ttggagttga aatcctttaa ttattcaaat gtcaatacgt gttttaaatt ttctccatac
      841 ggtattataa tggaaaattt taaaaattgg gaaagcacat tccaaacgct tttctataca
      901 aaaaaagagg cagaacgagc ttacaaattg acatctgagg ctatctacaa atcgaaatac
      961 gtaaagcatg aaaatgcata tattcagggt attaatctat tcaaacaaat tcaattcaac
     1021 ttattgaata ccaataaagg ctgatagttg cactgatcaa tgagataata aattctcatg
     1081 taaacaagta aattccctgt ttactgcatt actttataca gaattactgc catttttgaa
     1141 tattctatct tcaccccttc ctgctttaat agtattcact ttatctttac cgtgagataa
     1201 gtctatttag tcatcccctt caatattttt cgtatttatc tgttgaatga tcagaatcat
     1261 tcataaataa acattatgta taatgaacac acacaacact ttaatttttg tatgtctata
     1321 tttctgtacc aatttattaa ctgctcaacc aattccatca tttcctggag ctgaagggtt
     1381 cggtgcacag gcaacaggtg gcagaggtgg cagagtaatc tacgtcacca acctcaatgc
     1441 caatgggcca ggaagcttaa atgctgcact ctctgaaagc ggcccgcgat acatactttt
     1501 taaggtaagt ggtgtcatag atgcagctgc tgaattaagg gaaggtgatg taactatagc
     1561 cggacaaaca tctccaggag gtataataac aaggggattt attgttgatc aggtgtatga
     1621 aactggtggt tcaggggata atattatcat gcgccatatc agatcaaggc cacatgaccc
     1681 ggcaattgtc ccgtcagatc attacgtgct ggacgatgct ctacggcttg acggtgcaag
     1741 caacatgatt gtagatcatt gttcattcgc caatgcccgt gacgaatgta ttcagatttc
     1801 ccaatcctct gacataacca tccagcatag tatgctttcg gaaactgtag gccctcatta
     1861 tatatacggc ggaatgctaa tcaattattc taccttggaa caccctcagg ataacctcag
     1921 tatccatcat aatacctgga ataggatagc gggtcgtttg cccgaagtga tttgtgaatc
     1981 accctattgc aactccgggc ctctacattt ggaactttca aataacctat tatgggaccc
     2041 tcaaaattat gtgtggtatg gagccaatat cgatccatca ggcgaagatt actcattttt
     2101 cgtccacatg aactgggtaa acaactttat gaagacgcgt acaacctttc cttatgcaat
     2161 gatggaaatg agatttcttc aatatgcaca gaatcagata tatggcttgg gcaacaatat
     2221 ggatatctat ccaatgtata gcgactatca gttattctat tgttgcaatg attttaatca
     2281 gccaggcaac aatccgaaca atgatccggg aatagcgcag ctactcagta atagacataa
     2341 cttcccatca attacttatg atccatcaaa taaccttgtc gattacatga ttgcgaactg
     2401 tggggctttc cctagggatc caatggacac aagactcatg aatccgttag tacaaaatat
     2461 tatagaacct ctaccggtag atggagtgga ttattttaat gatgctttta taactaatgc
     2521 tcccacaacg ccccctgatg atactgatat ggatggaatg cctgattact gggaactggg
     2581 gcacggattg aacccgtcaa ttcaggacca caatggcaca cagttatctt taagtatcac
     2641 gggtgtctca ggatatacca acctggaatg ctacctcaat tgcctgtcag atggattggt
     2701 caacggccag tcaacgccgt catgtgggat agctctgcct gttattttga aaaatttcga
     2761 ggtaaaaaaa tatgatcaaa ccgtaatatt aatgtggaag gttacagaag aaagaaattt
     2821 tagtggatat tcaattgaaa gaagtaaaga cggaaaggtc tggacaacta tcggttatgt
     2881 agaagcctcg acaaaagaga atgccaatgc aaatgaagta acctatgatt acatggatga
     2941 tcaaccaatg ccgggggtta attactacag aattaaaatg tcagacaagg atgggagtat
     3001 ccaatattct ttagtgaagc aggcaacgtt tcaaaatcat gctgaaatag atatttatcc
     3061 taatccgact accggcatta tttatttcaa tggactgaaa ggcaaggctg atgttcaaat
     3121 agctgatcaa ataggaaaaa tccatatatc aaccacaata cataatacat caaataaact
     3181 agatgttagt aatttgatca acggaatgta ctttatcaaa gtaacaaccg aatatggaaa
     3241 tactatcaca aagaaggtta tgaaaaacta aaaagcccac aaacaattaa ggccctcaca
     3301 aaagggcctt aatatttttt acctgtatag tttgtcggaa ctactggatt cgaaccagtg
     3361 acctcttccc tgtcaaggaa gcgctctaaa ccaactgagc taagctccga aaaaaaaggt
     3421 tatcgcaaga taaccctttc taaaaaaacc tgaagttttt agattacttc tgcatagtag
     3481 tatcagcagc aggtgcagct gttgtgtcag cagcaggtgc agcttcctca ggtgcaggtg
     3541 cagctgttgt gtcaacagtt gtagtttcag gctcagttgt agttgtctcg ctcttgcaag
     3601 aagtcatagc gaaagaaaca gatcctacaa caatcaaaga aagaattaat tttttcattt
     3661 ctaaataaag tttaaaatta tgttaaaact gactacaaag ataaggcact tttttatatc
     3721 tgcaataatt ttattatttt ttttagcaag ctggaacact tatctaccaa gccatttgag
     3781 caaataacac attttaataa tgttaatatt tattaaacaa aaagctaaag tgagtctgat
     3841 tttcttaaca caattttaac gctaatgtta aaattgataa ctataagaaa tctacatcct
     3901 gtacataagg gtacctcatc aggaatcgta ttgcagtttg caggttataa ttttcaaaga
     3961 caaccttatg taagagagta attattggtg catttacttt ataatgtttg ataagaccgt
     4021 tggtaatctt caatactctg accccttcgg ctaatccgtc catacttgcc gcaatctgct
     4081 cttttgtttc acccattgca agcctccgac cgaaggtata attacggctg gatggactag
     4141 tggcagttgc aactaaatca cctatgccgg ccgtaccaag gaaggccctc gccgggacat
     4201 ccatagcctt gcctatatat atcaattcgc gcagtcctcg ggtgatcagc aatgcttcca
     4261 gattctttcc cattcctata ccgttaagca tacctgtaca aagcgcaatg acatttttaa
     4321 gtgaaccggc aagttcagca ccgagtaact ctggagatcc aaagactgca aagtcagggc
     4381 cat
//
LOCUS       DAOQQN010000285         4344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9483_length_4344_cov_6.123339, whole genome shotgun sequence.
ACCESSION   DAOQQN010000285 DAOQQN010000000
VERSION     DAOQQN010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9483_length_4344_cov_6.123339"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PLG24_10245"
     CDS             complement(<1..817)
                     /locus_tag="PLG24_10245"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HMZ25315.1"
                     /translation="MTLPKIGFSIGDTNGIGLEVIIKSLLSNRFSNRFIPVIFGSPKI
                     INYYRKMLSIDDNIFQFLKVGENPRPKKVNIFTCWEEEATISHGEVSREGGKFAKLSL
                     EQAIESLKSKYIDSLVTGPIHKKSMELAGFGFPGHTEYLEKSFPPQQSLMLMVYEDLR
                     IGVVTGHIALHEVASGINPKAIITKAKIMLQSLKVDFGIEKPVIAVLGLNPHAGDKGM
                     FGNEEENIIIPAINDLKKDGHIVMGPYPADGFFGSGLFKKFDGILAMYHDQGLV"
     gene            1547..3292
                     /locus_tag="PLG24_10250"
     CDS             1547..3292
                     /locus_tag="PLG24_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HMZ25316.1"
                     /translation="MKRSFFLAVLCLIGWRGSSQETFPIYNFKQEVEENRSVTYDKAI
                     ELWKIMSLYDKRLVVRETGKTDVGRPLHEVIVDGDSDFDPVKARRRTKNVIFIINGIH
                     PGEPDGIDATLWLVQDLLTDPKKGELLKNVVLVIIPVYNVGGALERNSTLRVSQNGPE
                     WYGFRGNGQYLDLNRDFIKCDSKNALSLVSIFTKWDPEILIDNHVSNGADYTYVMTML
                     VSQPDKLGHGQDAFLRQKMLPDLYRRMEKKGFEVPPYVNVWRSTPDKGLPGFMDTPRY
                     SSGYGSLFQTYCFVPESHMLKPYDQRVKATYAFMMSMLEYHAKNFIDIKISRSKAINE
                     VRDAARLPVLWSFDRSRADTFLFKGYDYEYIASEVSGLLRLKYRRDKPVTWKVPFWNY
                     ADAVVEVDVPKAYIVPQAYARVVERLDANRVKYLPIRQDTIIKGKMYRIVSTDSPKEP
                     YEGHFGHSNTRVEEFEAEKKFLRGDLIVYTDQRTRRYLVETLEPQGHDSFFNWNFFDG
                     ILDHKEGFSDYVFEDVAAEMLRNDPDLKKSLEQAKENDPKLRESAYGQLNYIYEHSKY
                     KEPWQNVYPVMRVMN"
     gene            3388..3702
                     /locus_tag="PLG24_10255"
     CDS             3388..3702
                     /locus_tag="PLG24_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HMZ25317.1"
                     /translation="MEGSRNKELDWLRIIAWGEGTSFLLILFVSMPLKYIWEMPMANK
                     IIGYVHGVLFILYIITVWLIAEKREWSTRVRVISLVASLIPFGAFWADRRIFARKVDS
                     GK"
     gene            4035..4108
                     /locus_tag="PLG24_10260"
     tRNA            4035..4108
                     /locus_tag="PLG24_10260"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4069..4071,aa:Thr,seq:tgt)
     gene            4122..4204
                     /locus_tag="PLG24_10265"
     tRNA            4122..4204
                     /locus_tag="PLG24_10265"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4156..4158,aa:Tyr,seq:gta)
BASE COUNT         1291 a          761 c         1030 g         1262 t
ORIGIN      
        1 gaaccagccc ctggtcatga tacatggcaa gtataccatc aaattttttg aaaagtcccg
       61 aaccgaaaaa cccatcagcc ggatatggtc ccatcacgat atgaccatcc ttttttaaat
      121 cgttaatagc aggaatgata atgttttcct cttcattgcc aaacattccc ttgtctccgg
      181 catgaggatt taaacccaat acagcgatta caggcttttc gattccgaag tcaactttta
      241 gggattgaag cataatcttt gccttggtga taattgcttt cggattgatc ccggaagcaa
      301 cttcgtgtaa ggctatgtgt ccggtaacaa caccgatacg aaggtcctcg tataccatca
      361 gcatcagtga ttgctgggga gggaaggact tttccagata ttctgtatgt cccggaaaac
      421 cgaatcctgc caactccata gattttttat ggatgggacc ggtaaccagt gaatctatat
      481 atttactttt aagactttca atggcctgct caagtgaaag tttggcaaat tttccacctt
      541 cccggctaac ctctccatgg ctgatagtcg cctcttcttc ccaacaagta aatatattta
      601 ctttctttgg ccttggattt tctccaacct taagaaactg aaatatgtta tcatcaatgg
      661 atagcatctt gcgataataa ttgattatct tgggcgagcc gaaaataacg ggaataaaac
      721 ggtttgaaaa acggttgctg aggagggatt tgataataac ctcaaggccg atgccatttg
      781 tatcacctat agaaaatccg attttaggaa gtgtcatccg gaaaaatttt gtgtcgcaaa
      841 tatagaagtt tcctccgtat aggttaagat gtatccgtaa catttccaaa ttcaatcaat
      901 aatcaattga aaataatggg gttgggaagg caggtcaaag tagatgtaat aaatgtaaac
      961 aagggctaac tcaatcaaac ttcattgatg accagcccta ttctgatttg agctgttgaa
     1021 cagggcacgg acccaaaaga gcaaaattat tcgagatata aaaaattgat gtattcaggt
     1081 gggcctgcat tcgtttgatc tgctcaaaga tggaatttag ctcaagcgga gcatgcaatc
     1141 tatatctatg atcaaagaaa cacatgagct tgttgccgaa aatggatggg gtaatccgga
     1201 atgattaatt tattcactgt tcgaaggggg agaatggatt ggggaacagt attccataga
     1261 gaatttgata tgtagttata ataaaaacaa gaaaaattcc cgggtagcgt tttttagcgg
     1321 cacaatttct atgattcctg tgaaatattt gcttttagag aaattcaatt taccaatgat
     1381 tgcaactgta tcggacaagg tcactattgg aggagtcgct aagtatgctt gtcagtatat
     1441 tgaataatta tgactcaatg atggaacgtc gtttaacagt gctgcacaga taatactttt
     1501 gaagtaaatg atttttggat aaattgcagc cttaaattct ctaaaaatga aaagaagttt
     1561 ttttttagca gtactttgct tgattggatg gcgtggtagc agccaggaaa ctttcccgat
     1621 ttataatttt aagcaggaag tagaagaaaa caggtcggta acttatgata aagctattga
     1681 attgtggaag ataatgtctt tatatgataa gcggttggtg gtaagagaaa caggcaagac
     1741 agatgtaggc cggcccctgc atgaggtcat cgtcgatggt gatagcgact ttgatcctgt
     1801 caaggctcga cgcaggacca agaatgtcat ttttattatt aatggtattc atcccggaga
     1861 gccggatgga attgatgcta ctttgtggct ggttcaggat cttttgaccg accctaagaa
     1921 gggtgaattg cttaaaaatg ttgtattggt gatcattccg gtgtataatg tcggtggtgc
     1981 tctggagcga aactcgactc ttcgtgtcag ccaaaacgga cctgaatggt atggcttcag
     2041 aggcaatgga caatatctgg atctgaatcg agactttatc aaatgtgatt cgaagaatgc
     2101 gctaagcctg gtgtccattt ttaccaagtg ggaccctgag atcctgattg ataaccatgt
     2161 ttccaatgga gctgactata catacgtgat gaccatgctg gttagccagc ccgataaact
     2221 ggggcatgga caggatgcgt tccttcgtca aaaaatgtta cctgacctgt ataggcgtat
     2281 ggagaaaaaa ggatttgaag ttccacctta tgtcaatgtg tggcgatcaa cacccgataa
     2341 gggtctcccg ggattcatgg atacacccag atattcgtcg ggctatggat ctttatttca
     2401 gacttattgt tttgttccgg agtcgcacat gttgaagcct tatgaccagc gggtcaaggc
     2461 tacttatgct tttatgatgt ccatgttgga gtatcatgct aaaaatttca ttgacatcaa
     2521 gataagccgt tccaaagcaa tcaatgaggt gcgtgatgct gccaggctgc cggtgttgtg
     2581 gagttttgac agaagcaggg cagacacctt cctgttcaag ggatatgatt atgaatacat
     2641 cgccagtgaa gtttcagggt tgttgcgatt gaagtatcgc agggataaac ccgttacctg
     2701 gaaggtccca ttctggaact atgccgatgc tgttgtggag gttgatgtgc cgaaagcgta
     2761 tattgtccca caggcgtatg caagagtcgt tgaaaggctc gatgctaatc gggttaaata
     2821 cctgcccata aggcaggata caatcataaa gggcaaaatg taccgaattg taagcacgga
     2881 cagcccaaaa gagccgtatg aagggcattt tggccactcg aacacaaggg tagaagaatt
     2941 tgaggcagaa aagaagtttt tgagaggaga cctgatcgta tatacggatc agcgtaccag
     3001 aaggtatttg gttgagacgt tggagcctca ggggcatgac tcattcttca actggaattt
     3061 ttttgatggt atattagacc ataaagaagg attttcggat tatgtatttg aagatgtagc
     3121 ggctgaaatg ttgcggaatg atcctgattt gaaaaaatcc ttggagcagg ctaaggagaa
     3181 tgatccaaaa ttgagagaaa gcgcttatgg acaattgaat tatatttacg agcattccaa
     3241 atataaggag ccctggcaga atgtctatcc ggtgatgaga gtcatgaatt agacttgtat
     3301 ttgtttatga aaagctcaaa aaaagacata aatattgaac ttcatgccat cctctttgtt
     3361 aagggagggc agtgaaacta agatttaatg gaaggaagta gaaataaaga attagactgg
     3421 ttgaggatca tagcttgggg ggaaggtaca tcatttctgc ttatactctt tgtatccatg
     3481 ccgttgaaat atatttggga aatgccaatg gccaataaga tcataggcta tgtacacggt
     3541 gtactcttta tcttatatat aatcacggta tggctgattg ctgaaaaacg tgaatggtca
     3601 accagggtta gagtcatatc tttggttgca tccttgattc cttttggggc attttgggcg
     3661 gataggcgta tatttgcccg gaaagtcgat tccggcaaat gatggatgta tggatttgtt
     3721 ctgcaatcct attacaaatg ttggacagga atttcaggca atcgataatt catgaaatgg
     3781 tattatgttg ataatcatga tgttgtgtat taataaagtt gtctggccaa aaaaaataaa
     3841 aaaaaattga ttttttttca agaggatatt tgaaatatca aagttagtcg tatctttgca
     3901 gtcgcttatg aaaaagttca taggaagtga ttgaaaagac cgaattggcg ctaatattgc
     3961 gcttgaaaat aaagtaagtt gaaatccttg tactttggcc ggaaagctcc gataaaagta
     4021 gaacgaaatt caatgccagt gtagctcagt tggccagagc agctgatttg taatcagctg
     4081 gtcgggggtt cgaatccctc cactggctct gtttatgtgt agggtagatg gccgagtggt
     4141 taaaggcgac agactgtaaa tctgttctct cacgagtacg gaggttcgaa tccttctctg
     4201 cccacatagt aagctgacag ttagcagatt acagtgtcca taggattact gtcggctgct
     4261 agctgtcagt tctagcttag ataagcggga gtagctcagt tggtagagcg acagccttcc
     4321 aagctgtagg tcgcgggttc gaac
//
LOCUS       DAOQQN010000286         4330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9522_length_4330_cov_5.238363, whole genome shotgun sequence.
ACCESSION   DAOQQN010000286 DAOQQN010000000
VERSION     DAOQQN010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9522_length_4330_cov_5.238363"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLG24_10270"
     CDS             <1..316
                     /locus_tag="PLG24_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25318.1"
                     /translation="LKEIEVRKQNYYKKKMAECHANALGMASTMVDSALIEQALFIGA
                     DTLLRPDRPMRPEVETFASNIENIPVRPWLKKTDFVSPFQKKDTFVKDSLKLKDSVKV
                     IK"
     gene            452..2140
                     /locus_tag="PLG24_10275"
     CDS             452..2140
                     /locus_tag="PLG24_10275"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMZ25319.1"
                     /translation="MEVNSFEDLNLEDSLLKALKAIDYSVPSPIQARAIPVALTGVDI
                     IGQAQTGTGKTAAFGIPMIQNISKSGRYTKGLVLCPTRELCIQVTNELKKLAYFSRHV
                     SVFAIYGGDPINPQIKALKAGVDIVVGTPGRVMDHLSRGTLLLDGLEILTLDEADEML
                     NMGFREDLETILQSVPEHRQTILFSATMPKAILDITHLYQKNPQLIKVTPTNLTSASV
                     EQFYIETDMNHRLKVLTYLLNAGDYAQAIVFCNTKAKVDELSNELAENLVKNDVLHGD
                     LGQEKRNRILNKFRKGEFKVLVATDVAARGIDVPNVDLVCNYDVPFDPEYYVHRIGRT
                     GRAGKEGKAYSFLAGSRDKRLLRQIESFAKVKVEQYKLPTATEIARIELDKVKTQLKA
                     AILAGKEAVYLDLAKELMQEGGPANIIAALLLKIIPPGKHEWAVSAGASRDNKEKRGE
                     RPDSNRRKQDRERTRRDEKPKPSANRGKDKDRKSDKPKFGKPEKTFVKHENEMSLEDI
                     LASLGLDSEGNDIKPSGKDKKKTDKKKMPGDHFYSSSIREKEEFVPVPRKKKKK"
     gene            2207..3070
                     /locus_tag="PLG24_10280"
     CDS             2207..3070
                     /locus_tag="PLG24_10280"
                     /inference="COORDINATES: protein motif:HMM:NF025625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HMZ25320.1"
                     /translation="MKFGKTIKIFLIDGDPNGRMSCELSNWTGKAFKIPRIKIKDCVD
                     RPEIKNPGIYFLFGKNEIGKDIIYIGEAETVYSRLLQHLNQKDFWNEAIIFISKDENL
                     NKAHIKYLENKLYEIAKQVNRYHIDNTTVPTLSSISESDRAEMEEFLENVKLLVSTLG
                     HKAFDTKRETSSIKPKDVFYLKGPRGADAHGLPTSDGFLVLKGSKAAESTTPSIASNL
                     VKLRSKIIDQGAIILKKGYFEFPDDFIFSSASAAASIVLGRNANGLSEWKFQSGKTLK
                     DFETNDAINEV"
     gene            3427..>4330
                     /locus_tag="PLG24_10285"
     CDS             3427..>4330
                     /locus_tag="PLG24_10285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMZ25321.1"
                     /translation="MTNKIIFSLVMAVSFQWSLSAQDVHFTRFYDAPTTLSPAKTGDY
                     LGTFRAGGIMRDQNYNLSHIYLTPAGYVDAPIIRGFRQNHWVGVGLTFLMDRAGAAGL
                     KNASFGASAAYHIGFNKKMTSYLSVGISYALAARSIADREKAIFEDGLINSIPSVDID
                     NINDQKVNYGFFSGGAQYTAALPNDGNIKAGFAMLYLNRPRNGILKTGGIRLPIRTNV
                     YATADASVADRIDILPALYFATMAGQRDMALQFQGGYRLNPRKGQRVMAGLGYRFGDA
                     IQVLAGMDIKNTRIGLAYDITVNDI"
BASE COUNT         1356 a          863 c          984 g         1127 t
ORIGIN      
        1 attaaaggaa atagaagtga ggaaacagaa ttactacaag aagaaaatgg cggagtgcca
       61 tgccaatgcg ttgggaatgg cttccacgat ggtggactct gctctgatcg agcaggcatt
      121 gtttatcggg gccgatacgc tactcagacc ggacaggcct atgaggccgg aagtcgagac
      181 ttttgcttca aatattgaaa atattccggt acgcccatgg cttaaaaaaa ctgattttgt
      241 cagtcctttc caaaaaaagg atacctttgt gaaagattcc ttgaagctga aggatagcgt
      301 gaaggtgata aaatgaattt tcaggtttat tgattgtcgg gctgcacttt gactaaagac
      361 gcaaaatctg attgtctcgt aagacaatcg ttaacatgta atacatttta ttggaaagaa
      421 atattccaac acaaccacca aatataaatc catggaagta aattcatttg aagacctaaa
      481 cctggaggat agcttgttga aagcattgaa agcgattgac tattcagttc ccagtccaat
      541 acaagcccga gctataccgg tcgccctgac aggggtggac ataatcgggc aagcacagac
      601 aggaacaggc aagacagccg ctttcggtat tccgatgatc caaaatatct ccaaatccgg
      661 ccgctatacc aaaggtctcg tcctatgtcc cacgcgcgag ttatgcattc aggttaccaa
      721 tgaactgaag aaactggcgt atttttccag gcatgtttcc gtttttgcaa tatatggtgg
      781 cgatcccatc aatccgcaga ttaaggcctt gaaagccgga gtggacattg tggtgggtac
      841 accgggaaga gtgatggacc acttatcacg agggacatta ttactggatg gattagaaat
      901 actcaccctg gatgaggcgg atgaaatgct caatatgggc tttagggaag accttgagac
      961 gattttgcaa tcggtaccgg agcataggca gaccatttta ttttcggcta ccatgccaaa
     1021 ggctattctg gatattacac acctgtacca gaagaatccc caactgatca aagtcacgcc
     1081 taccaatctt acctcagcaa gtgtagaaca attttatatt gagacagaca tgaatcatcg
     1141 cctgaaagta cttacctatc tgctcaatgc aggcgattat gcccaggcca tcgttttttg
     1201 taacaccaag gccaaggtcg atgaactgtc caatgaattg gccgaaaatc tggtaaaaaa
     1261 cgacgtcttg cacggagatt tgggccagga aaaacgcaat cgcatcctca ataagttcag
     1321 aaaaggagag tttaaggtat tggtggctac agatgtggca gctcggggga tcgatgttcc
     1381 caatgtcgat ctggtctgta attacgatgt gccgtttgat ccggaatatt atgtacacag
     1441 gataggcagg acgggccggg ccggtaaaga gggcaaggca tatagttttc tggcaggctc
     1501 aagagacaaa agactgctcc gtcagattga gagctttgcc aaggtcaaag tagaacaata
     1561 caaattgcct acagccacgg aaattgcacg aatcgaactg gacaaggtaa aaacgcaact
     1621 gaaggctgct atcctggccg gaaaagaagc cgtatatctg gatctggcaa aagagttgat
     1681 gcaggaaggt ggaccggcga acataattgc tgcccttctt ttgaaaatta tacctcccgg
     1741 caaacacgaa tgggcagtaa gcgccggtgc atcgagagac aacaaggaga aaagaggtga
     1801 acggccggac tcaaacaggc ggaaacagga tcgggaaagg acccggagag atgaaaagcc
     1861 taagccatcc gcaaatcgcg gcaaagataa agatcgcaaa tcagataaac ctaagttcgg
     1921 aaaaccggaa aaaacttttg tcaaacacga aaatgagatg tcattggagg atatcctggc
     1981 cagtttaggt ttggattccg agggtaatga tatcaaacct tccggtaaag acaaaaagaa
     2041 gactgataaa aagaaaatgc cgggtgatca tttttatagt tcatctatac gagaaaagga
     2101 agaatttgta cctgtgcccc ggaagaagaa gaaaaaataa tgctctgaca ggtgattggt
     2161 ttttctgttt gatgatttag cgctttgtaa taacaaatac tcaagaatga aatttggcaa
     2221 gactataaaa atatttctaa ttgatggtga tccaaacggg cggatgagtt gtgaactttc
     2281 taattggaca ggaaaagcat ttaaaattcc ccgaattaaa attaaagatt gtgttgacag
     2341 gccggaaatt aaaaatccgg gaatttactt tctatttgga aaaaacgaaa ttggaaagga
     2401 tataatttac attggtgaag ccgaaacagt atatagccgt ttgcttcagc accttaatca
     2461 aaaggatttt tggaatgagg ccataatatt tattagcaaa gatgaaaatc ttaataaggc
     2521 tcatattaag tatttagaaa acaaattgta tgaaattgcc aaacaggtaa atagatacca
     2581 tatagataac acaaccgtac caactctgtc gtcgatctcc gagtctgatc gcgcagaaat
     2641 ggaagagttt ttagaaaatg taaagctttt agtaagtaca cttgggcata aggctttcga
     2701 cacaaaaagg gaaacttcct cgatcaaacc caaagatgtc ttttatttaa aaggacccag
     2761 aggagcagat gcacatggat taccaacctc tgatggattt cttgtattga aaggttcaaa
     2821 agcagcggaa tcgaccactc cttcaatcgc ctccaacttg gtgaagctta gatcaaaaat
     2881 aattgatcaa ggggctatta ttttaaaaaa aggatatttt gaattccctg atgattttat
     2941 attcagtagt gcttccgctg cggcttctat agttttaggt agaaatgcta acggattaag
     3001 cgagtggaaa tttcaaagtg gtaaaacact taaagacttt gaaacaaatg atgcaataaa
     3061 cgaggtataa caaggtactt agtcaaaacc ggcttttcgt tttaaatgaa aatcttaact
     3121 taacaatccg gcactgcgta agcacccgac cgttttgcgc aacattttta atattttaac
     3181 acgtgaggca acaggagctt gtatgcaagt gtcatttgat tcaatttgcc tcccatcatc
     3241 catgtaattt ttttacaaag aaaaggatgt gttgaaaaac ataaaatatc cgggtacacc
     3301 gaaggatgaa actaaaagaa gccatgtcaa tgccttgaaa tccatgctgt catattttat
     3361 ttttttcctt accataattt tatttacttt tatcccaatc ttatcgaatc cacgaaattt
     3421 acgtgtatga ccaataaaat tattttttca ctcgtgatgg cggtgtcctt tcaatggtca
     3481 ttgtcagcac aggatgtaca ctttacaagg ttctatgatg caccgaccac gctgagtcct
     3541 gctaagaccg gggattacct cggtaccttc cgggcaggtg gaatcatgcg tgatcagaac
     3601 tataatttgt cacacatcta tcttactccc gccggttatg ttgacgcgcc tatcatacgg
     3661 ggcttcaggc agaatcattg ggtaggtgtc ggattgactt ttctgatgga ccgggcagga
     3721 gctgccggat taaaaaatgc ctcctttggt gcatcagctg cctatcacat tggttttaat
     3781 aaaaaaatga cttcctatct ttctgtggga atttcctatg cattggctgc ccgttccatt
     3841 gcagacaggg agaaggcgat atttgaggat ggcctgatca attcaattcc atccgtggat
     3901 atcgataata tcaacgatca gaaagttaat tacggatttt tctccggggg agctcagtac
     3961 acggctgccc tcccaaatga cggtaacatc aaggccggtt tcgccatgct gtatctcaac
     4021 cgtccaagga atggcatcct gaagaccggt ggcatccggc ttcctatccg caccaatgta
     4081 tatgccacgg cggatgcatc cgtagcagat aggatcgaca tcctgcctgc tttatacttt
     4141 gccactatgg ccggccaaag agatatggca ctgcaatttc agggaggcta tcggctcaac
     4201 cctcgtaaag gtcagcgtgt aatggcagga ttgggctatc gttttggtga tgccattcag
     4261 gtactggcgg gtatggatat taaaaataca cggattggcc ttgcttacga catcactgtc
     4321 aatgatatcc
//
LOCUS       DAOQQN010000287         4312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9572_length_4312_cov_6.121212, whole genome shotgun sequence.
ACCESSION   DAOQQN010000287 DAOQQN010000000
VERSION     DAOQQN010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9572_length_4312_cov_6.121212"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            370..1608
                     /locus_tag="PLG24_10290"
     CDS             370..1608
                     /locus_tag="PLG24_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ25322.1"
                     /translation="MNQIENIRQAFKNIRSNKVRSILTILIIAFGIMALVGILTAIDS
                     ILNSMVTNFSGLGANSFSITQKNADFRSNRRGFREKPGKEINFAQAMMLFEKNDMPTT
                     ISISATGINGATVVYNGKKTDPVISIAGINENYMKVNALDLATGRAFSANEFLYGENV
                     CIVGNEVIKRLFDGRESSALDRVISAGGNRFRIVGVMTTQGSSMTSNMDNQIEIPLLT
                     QKKLYGYSDQNYNIAVSVPQGLNLDEAIDYTTGLFRNIRQLRVGQDNDFEIQQADAIM
                     NIIQENTVTIRIATIGIGLITLLGAAIGLMNIMLVSVTERTREIGILKALGATRNNIL
                     IQFLTEAIVICQLGGLVGIVLGILAGNSIALFLDGGFIIPWAWITLGIITCLLVGLAS
                     GLYPAMRAARLDPVESLRYE"
     gene            1711..2724
                     /locus_tag="PLG24_10295"
     CDS             1711..2724
                     /locus_tag="PLG24_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25323.1"
                     /translation="MKYILNLLFLFPWALFAQEDPEALVLRGVALYDQGKYPEALKVL
                     ERARDIWPRSSTILYEIALTRHSLRDYEGCHRLTDSIIVMRDNNMTQGYLLKASALKQ
                     LGKTIESSEVLIDGIRDTGGDEQLFYNLGINFITEKKFNEAEICFLNAINFKPTHSSS
                     YLQLAELNQDVNPTKALLAACQFLLLEPNSNRSKPAFELIEQILNGKSKSPSMKIQKE
                     DAENHIIRLDSSYIFSNGIIRLDAETNFNNNGIVDFDYFSAELKKVSLNCIDRIEYKE
                     HDVWRGYLIPFFADIFSSGFGDVFCNYIAQSRWPACSLWLNNRRSEFDRFTAFLKSEE
                     EKK"
     gene            2747..4159
                     /locus_tag="PLG24_10300"
     CDS             2747..4159
                     /locus_tag="PLG24_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMZ25324.1"
                     /translation="MKLSRRSLIRKLSIGMGVMSINLPGFANEFRDETREKKVNSSNS
                     FNMSGYAAPKMDRVRVGIIGLGNRGEEAVRRLCYIDGLDIVALCDKYSDRTEKCRTAV
                     VDKKLPDPKTFSGSEDAWKGLCDMDLDLVYICTPWHWHTPMAIYSMKAGKHVAVEVPV
                     TQSMDECWELVETSENTKKHCVILENCCYDFFEMLTLNMARQGLFGEIVHTEGAYIHD
                     LMDLNFSKTYYADLWRLKENATRNGNLYPTHGLGPVAQCLDINRGDRMEYLVSVSSGD
                     FSMHDREVKEAGKDPMFQPFINENFRGNMNTTTIKTEKGKTIMIQHDVSSPRPYSRIH
                     LISGTKGIAQKWPGPERIAFGHSWLKKEEMDTLYDKHTPPLIKHIGNIAKEVGGHGGM
                     DFVMDWRLIDCLRNGLPMDMDVYDAASWSAVGILSEVSVKKRSMPMLIPDYTRGNYKT
                     NEKVDLTLSKGVTTGVRKLG"
BASE COUNT         1362 a          813 c          919 g         1218 t
ORIGIN      
        1 tgaccatagg gaaagaaagt ttcagtgaat tcattttatc ttacttcggg tttttctgct
       61 taatctgatc tatacccaaa gtataatgct tttcatttct ggatttctgt ctattaattt
      121 catacctact tcgatttccc cctacactaa actgcacgac acagcggaat cagaagcttt
      181 caatactaaa tcaaagcttt ttgtacccgg ctttccgaat gattatttta aatccgatta
      241 aactgtaaaa gaaatttaaa ggctaaatga atgaacattt atcggagaaa attgacattt
      301 gggcaaaagt aatttatagt tccatcttat aattttacct ttagaacgtc aaaagaggaa
      361 aaacaagatt tgaatcaaat tgagaatata aggcaagctt ttaaaaacat tcgttccaac
      421 aaggttcggt ccatattgac tattctcatc atagcattcg ggattatggc attggttgga
      481 atcctaacag ctattgactc catattgaat tccatggtga ctaacttttc aggattagga
      541 gctaacagtt tttcaatcac ccagaaaaat gctgacttcc gaagcaatcg tagaggattc
      601 agggaaaaac ctggtaagga aattaacttc gctcaggcaa tgatgttatt cgagaaaaac
      661 gacatgccga ctacaatttc catttctgct accggcatca atggtgcaac agtagtttat
      721 aacggaaaga aaacagaccc ggtcatctct attgcaggca ttaatgagaa ctatatgaag
      781 gtaaatgcgt tggatcttgc aaccggaaga gcattttcag ccaatgaatt cctttatggc
      841 gaaaatgtgt gcatagttgg gaatgaggta ataaaaaggc ttttcgatgg aagggaatca
      901 tcagcacttg atcgagtgat ttcggcagga ggaaacagat tcagaattgt cggagtgatg
      961 acaacacaag gaagttccat gacctccaat atggataacc agatagaaat accgttactt
     1021 acacaaaaaa aactctacgg ttattcagac caaaattaca atattgctgt cagtgttccc
     1081 caaggcttga atcttgatga ggctatagac tacaccacag gattattcag aaatatcagg
     1141 cagctcagag ttgggcaaga caatgacttt gagattcaac aggctgatgc catcatgaat
     1201 atcattcagg aaaatacggt tacaataaga atagcaacca tcggcatagg gttgatcacc
     1261 ttattgggtg cagcgatcgg cttgatgaac attatgctcg tctctgtcac ggaaagaacc
     1321 cgagaaattg gcattctcaa agcattaggt gcaacacgta ataatattct catacaattt
     1381 ttgactgagg caatagtgat ctgtcaactg ggtggcctgg tcggtattgt gctcggaatt
     1441 ttggccggaa attccatagc gttatttctc gatggtggct tcatcattcc ttgggcatgg
     1501 ataacactcg gtatcatcac ttgtctttta gtcggtttgg catcagggct ttaccctgcg
     1561 atgcgagccg ccaggcttga tccggtggag agtctgagat atgaataaag aaacttctct
     1621 taagaaaaag aaaaaggata gtaaagattg tttctgaacc ttcaatggtc ggaagaaata
     1681 tgttaacttt ccgcttcgtt ttcattggta atgaagtata ttctcaattt attgtttctc
     1741 tttccatggg cacttttcgc tcaggaagat cccgaagctt tggtgcttcg gggagtcgcc
     1801 ctctatgatc agggaaaata ccccgaagca ttgaaggtat tggagcgggc ccgcgatatc
     1861 tggccaagat catcgactat tctttatgaa atagctctaa caagacatag cctcagggat
     1921 tatgaaggtt gtcatcgcct tactgacagc ataatagtca tgagagacaa taatatgaca
     1981 cagggctatc tcctcaaagc atcagcctta aagcaacttg gtaagaccat agagtcttcg
     2041 gaagtcctca ttgatggcat cagggatacc gggggagatg aacaactttt ttacaatctt
     2101 ggaatcaatt ttattactga aaaaaaattc aatgaagcgg aaatatgttt tttaaatgca
     2161 atcaatttca agccaactca ttcctcttca taccttcaat tagctgaatt gaaccaagac
     2221 gtaaatccca ctaaggcatt attggcagct tgtcaatttt tattactcga accgaattcc
     2281 aatagatcca aaccagcttt tgagttgatt gaacaaatcc tgaatggcaa atccaaatcg
     2341 ccctccatga aaattcaaaa agaagatgct gaaaatcata ttatcaggtt agacagtagc
     2401 tatatttttt ccaatggaat catacgactt gatgcagaaa caaattttaa taataatgga
     2461 atagtggatt ttgattattt tagtgctgag ctaaaaaaag tttccttgaa ttgtattgat
     2521 agaatagaat acaaggagca tgatgtgtgg cgcggctatc tgatcccttt ctttgcagat
     2581 atattcagca gtggattcgg agatgtattt tgtaattata tagcccaatc acgatggcca
     2641 gcttgttccc tgtggctcaa caaccggcgt agtgaatttg accgatttac ggcatttttg
     2701 aaaagtgagg aggaaaaaaa ataaatcatt tttaaaataa agaaagatga aattatcacg
     2761 tagaagtcta atcaggaaac tttcaatcgg aatgggtgtc atgagcatca atctgcccgg
     2821 gtttgcgaat gaatttcggg atgaaactcg tgaaaagaaa gtaaacagtt caaatagttt
     2881 taatatgtcg ggctatgcgg cccctaagat ggacagagta cgtgtaggca tcataggcct
     2941 gggaaatcgt ggcgaggaag cggtgagacg tctgtgctac atagatggac tggatatagt
     3001 tgccttatgt gataaatact cagatcgcac tgaaaagtgt aggacagcgg tcgtcgataa
     3061 aaaattgccg gatcccaaaa ccttcagtgg aagcgaggat gcctggaagg gactctgtga
     3121 tatggacctt gatcttgtgt atatatgtac accatggcac tggcatacgc ctatggctat
     3181 atattccatg aaagccggta aacatgtcgc tgttgaggtt cctgtaactc aatcaatgga
     3241 cgaatgttgg gaactggttg aaacttccga aaacacaaag aaacattgtg tgattttgga
     3301 aaactgctgc tacgactttt tcgaaatgct gaccttgaac atggctcgtc aaggtctttt
     3361 cggtgagata gtacataccg aaggagcata tatacatgat ctgatggatt taaatttctc
     3421 caagacatac tacgcagatc tttggcgact caaggaaaat gccacccgta atggtaacct
     3481 atacccgaca catggcctcg gccctgtagc tcaatgtctt gatatcaacc ggggagatcg
     3541 gatggaatat ttggtatcag tcagctcagg agatttttct atgcatgaca gagaggtcaa
     3601 agaagctggt aaggatccaa tgtttcaacc ctttatcaat gaaaatttcc gtggtaatat
     3661 gaatactacc acgatcaaaa cagaaaaggg caagacaatc atgattcaac acgacgtatc
     3721 gtcaccgaga ccttattcca gaatacacct tattagtgga actaagggaa ttgcccaaaa
     3781 atggcctggt cctgaaagaa tagcctttgg acactcctgg ttgaaaaaag aagaaatgga
     3841 tacgctctat gacaagcata ctccaccact catcaagcat atcggaaata ttgctaaaga
     3901 agttggtggc catggcggca tggacttcgt gatggactgg agattaatag attgtctacg
     3961 caatgggtta ccaatggata tggatgttta tgatgctgct tcctggtctg ccgtgggaat
     4021 tctcagtgaa gtttccgtaa aaaagcgctc catgccgatg ctaattccgg attatacacg
     4081 cggcaactat aaaacaaatg agaaagttga ccttacctta tcgaaaggcg taacaactgg
     4141 agttaggaaa ctcggctgat aaaattggtc acagagattt gaatcagtta aatgaaatct
     4201 cttatggttt atgcatactt gttaatccct gacttataaa caaccatctt attagttgtc
     4261 atcggcctat gcctgaccat tctggagatg atgtcatcca ggatttcacc tt
//
LOCUS       DAOQQN010000288         4310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9578_length_4310_cov_4.827027, whole genome shotgun sequence.
ACCESSION   DAOQQN010000288 DAOQQN010000000
VERSION     DAOQQN010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9578_length_4310_cov_4.827027"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /gene="uppS"
                     /locus_tag="PLG24_10305"
     CDS             complement(<1..470)
                     /gene="uppS"
                     /locus_tag="PLG24_10305"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HMZ25325.1"
                     /translation="MSTPDHFEYIDKSKLPRHVAIIMDGNGRWAKQQGKSRVLGHSAG
                     VKSVREVSEAAARLGIEYLTLYAFSTENWNRPKLEVEALMLLLVDTINKEIATLKKNN
                     IRLNAIGNLAGLPTASQKALKKGIDATKDNDGLNLILALNYSGREEITNMVKLIS"
     gene            623..1171
                     /locus_tag="PLG24_10310"
     CDS             623..1171
                     /locus_tag="PLG24_10310"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HMZ25326.1"
                     /translation="MKIFQYIIFFILAVLVYILYQYMYMKPAFMVGEKAPSFRAQTID
                     GKHFSLDDMSGKLVLLDFWGTWCSPCRKQLPELSKLYRTYRDSLHGIFEVVSVAMDDD
                     SAILRQVINSDSLAWPSHFREPKDGGPITTLYQIKKVPTSYLINDKGQIISVNPTIDD
                     LKSWLMKIDQAHYSKVRSQGVH"
     gene            1887..3023
                     /locus_tag="PLG24_10315"
     CDS             1887..3023
                     /locus_tag="PLG24_10315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ25327.1"
                     /translation="MPKNRILFLASWYPSRVSPYSGDFIQRHARVAARIADITVLHTV
                     RDDDIAGAYEVSDTDNGLREIIVYYKDSGCKVLNFFKRMKAFWMGYIRAEVPDLVHLN
                     TVLPAGLFALYLKFFLGKPFILSEHWTGFQPFRFRQLNLMTHILVRLILQNCRLVLPV
                     SADLGKQMKAVCSKPEFEVVPNVVDTDRFRIFSERKSSAKPRFLHLSMLDEQHKNISG
                     ILRSAKRLVDSGLSFELHIGGNGPLEPIRQFVARHHLQDVIFPFGELSHDEVPAMMAN
                     ADCFLLFSNYENQPCVQGEAFACGLPFIGTDVGGIGEFLPETFGILIPKGDEDALYRA
                     MESVIRGRKFESPAEMHRFAEENFSPEHLANRFHDIYNRVMHEK"
     gene            3013..>4310
                     /locus_tag="PLG24_10320"
     CDS             3013..>4310
                     /locus_tag="PLG24_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HMZ25328.1"
                     /translation="MKSEFQISHLPKKELTFREMVDGVQSFLYHFFDNPSYRFFLKGV
                     VLNFNELIASYGTAADIEHLVTALYEKLGTGFSREFSGEYSGYLYDKRNGKVFVYTNL
                     TASQTIFYYHRDDFLLVDTGLASLKRSLDRHSLLYSIDMEASYCTLALNSLPDNFTLI
                     SEVRKLKGGEQLAFDVETRKMEIQSWEGPGSQDLYRGDKKSALHTLDELFTRAVRQEY
                     TFDLEHQVGHFALLSGGLDSRMGVLYSRRMGFRHEQALCFSHSGYWDEIIARQIAGKE
                     GLTLSVVHLDGGEFLKHIDKLVGITQGLACYTGGVHSEFALSTIDFADKGLIHTGHLG
                     DAVLGSYLSSPARNPASSAFPKLIANQRYADKVRPVLDRIAAKYPSEEAFLFYNRGFN
                     FIAVGNYVAEQFSVAVSPFMHNDFLRFALSLDEAWRYKEK"
BASE COUNT         1094 a          855 c          976 g         1385 t
ORIGIN      
        1 gaaattagct ttaccatgtt ggttatttcc tctctgccac tatagttcaa ggcaaggatg
       61 agattcaggc cgtcattgtc ctttgtggca tcaatgcctt ttttcagggc tttctgactg
      121 gctgtaggga gaccagccag atttcctatg gcattgagtc tgatattgtt tttcttcaag
      181 gtcgctattt ccttgttgat agtatccacc aacaacaaca tcagtgcttc tacttccagt
      241 tttggtctgt tccagttttc ggtagagaaa gcatacaacg tcaggtattc aatgcctaac
      301 cgggccgcag cttcagaaac ttcccgcacg ctctttacac ctgcagaatg tccaagcacc
      361 ctcgacttgc cctgttgctt agcccaacgg ccattaccgt ccatgatgat ggcaacgtgg
      421 cgaggtagtt tacttttgtc aatatattcg aaatggtcag gggttgacat gagtaaatat
      481 ttatgcaaaa gtatcatttt atatcgtttt atgacgttat ccttgagata tcggatattt
      541 ttttgttcaa tgtttagaaa ataaaataaa tactgggcaa ttctcctaat attagttatt
      601 tttgtaatat aattccaggt tgatgaagat ttttcaatat attattttct ttattctggc
      661 cgtattggtc tatattctct accaatatat gtatatgaag cctgcattca tggtcggaga
      721 aaaggcgcct tccttccgtg cgcagactat tgacggcaag catttctcac tggatgatat
      781 gtcaggcaaa cttgtattgc ttgacttttg ggggacctgg tgcagtccat gccgcaaaca
      841 attgccggag ctttctaaat tgtataggac ttacagggat tcgttgcacg ggatttttga
      901 agtggtgtca gtagccatgg atgatgattc tgctattctg aggcaggtaa tcaactcgga
      961 cagcttagcg tggccctcac acttcaggga gccaaaggat ggtggcccga tcacgactct
     1021 gtatcagata aagaaggttc ctacctcata tctaatcaat gataaaggtc agatcatcag
     1081 tgtcaatccg acaatcgacg atctgaagtc ctggctgatg aaaattgatc aggcacatta
     1141 cagtaaggtg aggagtcagg gagtgcattg ataatgagtc agtaatgtgt cattgtacag
     1201 tcacatattt gtgctgtgat aattctcagg tcgtgactat ggtttctgga tactgtttct
     1261 gaatgaagct tggtcctaag gttattacaa ccagacttct tgttgtctaa tgttgcggtt
     1321 tttttccaaa tgattattgg ttgaggtttg tcagcaaatt aagatttcgg atttccaata
     1381 tatttttact gctttgtcag aagcaggtat aagatgtgaa ttcgtacaaa gcgtacaggt
     1441 aattccacct tactctcatc aatagattac atttgtaaaa aataaatata tataaaatgc
     1501 tttaatataa atatttaagt aattatttat actttgttga attgtaatat tcttttatac
     1561 ggcttttgcc tgtcatctcc acagctttat ttattcaacc ttcctttatt gtcccgttta
     1621 atatcaattt gctaaaagtt tagcaggata tttcgttgtc ttttaatggg ttggcaccaa
     1681 actcccttat agtaaacaga atattaatgg gtaaattgac ctattatcag gtagcaacca
     1741 aggatgaaat cattttttta catccccaaa gatcatgatc tgatgaccta ttgcagggca
     1801 aagagttata agtataattt cagctttttt acgtataccg ttactttagt tgctatcttt
     1861 gccaaaataa ccagttattt gggcgcatgc caaaaaaccg tattttattc cttgcatcct
     1921 ggtatccatc ccgtgtgtcg ccttattcgg gagattttat ccaacggcac gccagagttg
     1981 ctgcccgtat tgcagatatt accgttctgc atactgttcg ggatgatgac atcgcgggtg
     2041 cctatgaggt cagtgatacc gataatggcc tgcgtgagat catagtatac tacaaggatt
     2101 ccggttgcaa agtgctgaat tttttcaaaa ggatgaaagc cttttggatg ggttatatca
     2161 gagccgaagt tccggatctt gtgcatctga atacagtgtt gcctgccgga ttatttgccc
     2221 tctacctgaa gtttttcctt ggcaagccat tcatactgag tgaacattgg accggatttc
     2281 aaccattcag gtttcgacaa ctcaacctta tgacacatat cctggtgcgg ctcattctgc
     2341 aaaattgtcg attggttctg cctgtgtcgg ctgatctggg gaagcaaatg aaggccgtat
     2401 gttctaagcc ggagtttgaa gtggtaccca atgtcgtgga caccgatcgc ttccgtatct
     2461 tttctgaaag gaagtcttcc gctaaacctc gttttttgca tttgtcgatg ctcgatgaac
     2521 aacataaaaa tatcagtggt atcttgcgtt cagctaagcg cttggtggat tcgggtcttt
     2581 cctttgagtt gcatataggc ggcaacgggc ccttggagcc aatccggcaa tttgtcgcga
     2641 gacatcattt gcaggatgta attttccctt tcggcgagct tagccatgat gaggtccctg
     2701 caatgatggc caatgctgat tgtttcctgc ttttcagcaa ctatgaaaac cagccttgtg
     2761 tgcagggaga ggctttcgct tgtggtcttc ccttcattgg cacagatgtc ggcggtattg
     2821 gtgaattcct gccggaaaca ttcggcatcc ttatacctaa aggtgatgag gacgccctgt
     2881 atcgggcgat ggaatctgtg atacgggggc gaaagtttga gtcacctgct gaaatgcatc
     2941 gatttgctga agaaaatttt tcacctgaac atttggctaa tcgctttcat gatatctata
     3001 acagagtaat gcatgaaaag tgaatttcag atatcacatc ttcctaagaa ggaattgacc
     3061 tttcgggaaa tggtggatgg tgttcagtca ttcctttacc atttctttga caatccgtcc
     3121 tatcgttttt ttctgaaagg tgtggtgctt aatttcaatg aattgattgc ctcctatgga
     3181 acagctgcag atatcgaaca tctggttact gccttgtacg aaaagcttgg caccggattt
     3241 tcccgcgaat tttccggaga atacagtgga tatttatatg ataagcgtaa tggtaaagta
     3301 tttgtatata ccaacctgac agcatctcaa acaatttttt actaccatag agatgatttc
     3361 ttacttgtcg ataccggttt ggcttccctg aagcgcagcc tggataggca ctctctgcta
     3421 tattccatcg atatggaagc gtcctattgt acattggcgc tcaacagtct gccggacaat
     3481 ttcactttga ttagcgaggt aaggaagcta aagggtggag agcagttggc ttttgatgtt
     3541 gagaccagga agatggaaat tcaaagttgg gagggaccgg gtagtcagga cctttatcgg
     3601 ggagataaaa agtcagccct tcataccctg gatgaattat ttacaagggc agtccgtcaa
     3661 gagtacacct tcgatttgga gcaccaggtc ggacactttg ctttattgag tggtgggctt
     3721 gattcgcgga tgggtgtact ttattcgcgc cggatggggt ttcgtcatga gcaggcattg
     3781 tgcttttcac attcaggata ttgggatgag attatagcaa ggcagattgc cgggaaggaa
     3841 ggcctgaccc tttccgtagt tcatttagat ggcggtgaat ttctgaagca tatcgacaag
     3901 cttgtcggca tcactcaagg attggcctgt tataccggag gcgttcattc cgaatttgca
     3961 ttgtcaacta ttgattttgc agataaggga ttgattcata cgggacatct tggagatgct
     4021 gttttaggct cctacctttc atctccggcc aggaatccgg cttcttctgc ctttcctaag
     4081 ctcattgcca atcaacgata tgcggataaa gtgagaccgg ttttggatcg aattgctgcc
     4141 aaataccctt ccgaggaggc ttttttgttt tataatcgcg gcttcaattt tatcgctgtc
     4201 ggcaactatg tggcagagca gttcagtgtg gctgtctccc cgtttatgca caatgatttt
     4261 ttgagatttg ctctgagtct tgatgaggca tggagataca aggaaaaaat
//
LOCUS       DAOQQN010000289         4305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9594_length_4305_cov_4.590118, whole genome shotgun sequence.
ACCESSION   DAOQQN010000289 DAOQQN010000000
VERSION     DAOQQN010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9594_length_4305_cov_4.590118"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PLG24_10325"
     CDS             complement(<1..298)
                     /locus_tag="PLG24_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HMZ25329.1"
                     /translation="MAEKTENQLNIELTEEVADGIYSNLAIITHSHSEFVLDFVRVMP
                     NVPKAKVQSRIIMTPQHAKRLMHALRDNIARFEQSFGSIQDIENQTIPPMSFSVP"
     gene            459..1793
                     /locus_tag="PLG24_10330"
     CDS             459..1793
                     /locus_tag="PLG24_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HMZ25330.1"
                     /translation="MFLKIVKQPGEYESLYDTIRFPSGLNGWDPSAKLDSDQPGVFRL
                     PDFSGILEYKACRGTWLSVEGSSTGGEIPNRKITFKAGDTVSISIDSWKDWYSSSTHT
                     ATKQVYLLKSDFPMTPFNKKRRVWIYLPDDYDSSSKSYPTLYLHDGQNLFDRALSFAG
                     EWQVDEAMQNYAKQGIKDCIVIGIDNGGNDRIDEYTPYINPKYGGGNGEKYAEFLVNT
                     LKPFIDRHFRTLTEPEHTGIAGSSLGGLISYFTAIRYPNVFGRVGVFSPSFWYSDSLM
                     ADVKKFVPQSYQKFYFVAGANEDEDMVPDIDKYITMMKQQGMTESQIQKVIRQDGKHS
                     EWFWAREFPMAYYWLDLWGELSEVSAYTTEQNYITVIDSPSTRPTIKISDNLPVTAHL
                     AIINASSGKVEMSLKVKHGDVVVCNELINPQVYIAKIIGNGVNQSVKFLKIN"
     gene            1923..3017
                     /locus_tag="PLG24_10335"
     CDS             1923..3017
                     /locus_tag="PLG24_10335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ25331.1"
                     /translation="MKYLHLYIFVFIFQIADAQIITSSTNPTPGTVNKQIGVIGFDTN
                     EVKKTGNAQVWNFNNLTLTGLNITTSYSDVAGSPFINEFPGANIIQKTSNQPDAFFKA
                     DNSGMHFIGGVMSGPMFPNGFLAAKFTPVIAQYNLPMQYGNHSAEATSFFYPIPKELI
                     PDSLLNNIPFQLDSIRIRIEITRKYDCIGSGTIHLPLGSYSVVQVDFSFKPGQKLEVK
                     VPFLGWIDVSQFVNLGQFNQFIPETEGIYFLSPQHSGPIAVFNRNTITKRLESGLISS
                     LAINATNENIQKITFAVYPNPAGEYIEIRDFNLKYTDIIIIDSNGKVVKSLKTTNDKQ
                     INVKDLHPGVYYLNIFNNNRQIGFSKWIKM"
     gene            3023..3625
                     /locus_tag="PLG24_10340"
     CDS             3023..3625
                     /locus_tag="PLG24_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HMZ25332.1"
                     /translation="MEALQWSWKEFFTVFVSLFAVIDILGSIPLLISLKEKMGYIRSF
                     YVVLISGILMILFYFVGRPFLNLFGLDEHSFAVGGAIVIFLLALEMVLGRDIFHTDSN
                     PKAGTLVPIAFPLIAGAGTLTTIMSLKATYETRGKNELVILVAILANLIIVYIVLKSL
                     KWVQRVIGVSGLIAIRKFFGVILLAIAIQIFVKNITLIGL"
     gene            3767..>4305
                     /locus_tag="PLG24_10345"
     CDS             3767..>4305
                     /locus_tag="PLG24_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ25333.1"
                     /translation="MSVEVNNLTKIYGTQKALDNVSFSVSEGDILGFLGPNGAGKSTT
                     MKILSCFIPQTSGEATIGGKNVKSESLEVRRLVGYLPEHNPLYKDMYIKEYLLYIAKL
                     YGIARPFNRVKDMIELTGLGREQKKLISQISKGYRQRVGLAQAMIHNPEVLIMDEPTS
                     GLDPNQLMEIRNVIREIGK"
BASE COUNT         1336 a          841 c          839 g         1289 t
ORIGIN      
        1 taggcacgct gaaactcata ggtggaatgg tctgattctc aatatcctga atacttccaa
       61 aactctgttc aaaccttgct atgttatcac gtaaagcgtg catcaggcgt ttggcatgtt
      121 gaggtgtcat aatgattctc gactgaacct tagccttcgg aacattcggc atcactcgaa
      181 cgaaatccag gacaaattca gaatgactgt gtgtaatgat tgccaaattg gaataaatac
      241 cgtctgccac ttcctcagtc aattcaatat tcaattggtt ctctgttttt tctgccatta
      301 ttgttacttt tgaatgtcaa agataaaata aattgacaaa acgattccat gattcgattt
      361 ttagaaatat aataaacgat tgacatattg atatgaaatg gttgaatttt ctgtttacaa
      421 ttttcacttt cttgcctgct tatgatataa atgcgcaaat gtttcttaaa atagtgaaac
      481 aacccggtga atatgaaagc ctctatgata cgatccgctt tccttccgga ttgaatggat
      541 gggatccttc agccaaactt gattcggatc agcccggagt tttcagactt ccagattttt
      601 ccggcatctt ggaatacaag gcttgcagag gcacctggct gtcagtcgaa ggatcttcaa
      661 ccggaggtga aataccaaac agaaagataa catttaaagc tggcgatact gtttccatta
      721 gtattgactc ttggaaagat tggtacagct cctccacaca cacagcaacc aaacaagtat
      781 acctgctcaa atcggatttc ccgatgactc cattcaataa gaagagaaga gtatggatct
      841 atctgcctga tgattatgat tcttcatcca aaagttaccc gactctatat ttacacgatg
      901 gccaaaacct ttttgacagg gcactgagtt ttgcaggcga atggcaagta gatgaggcaa
      961 tgcagaacta tgcaaagcag ggcattaagg attgcatagt tattggaatt gacaacggag
     1021 gcaatgacag aatcgatgaa tacaccccct atataaatcc aaaatatggc ggtggaaatg
     1081 gtgagaaata tgccgaattt ttagttaaca ccctaaaacc tttcattgat aggcatttca
     1141 gaacattaac cgaaccagaa cataccggaa tcgccggttc atcattaggt ggcttaatca
     1201 gctattttac cgccatcagg tatccgaatg tttttggtag agtaggagtt ttttcacctt
     1261 ccttctggta ttcagacagc ttaatggccg atgtgaaaaa atttgttcca caatcctatc
     1321 agaaattcta tttcgtggca ggcgccaatg aagatgaaga tatggtacct gatatagata
     1381 aatacattac catgatgaaa caacagggga tgacagaaag ccagatacaa aaggttatca
     1441 ggcaagatgg gaagcatagt gaatggtttt gggcacgtga attccccatg gcttattatt
     1501 ggttggactt gtggggtgaa ttgtcagagg tatcagctta taccacagaa caaaattata
     1561 tcacagttat tgactcgcca tcgacccggc cgacaattaa gattagtgac aatctgccgg
     1621 ttacggctca ccttgccatt ataaatgcca gttcgggaaa ggttgagatg tccttaaaag
     1681 ttaaacatgg tgatgttgta gtatgcaatg agctgatcaa ccctcaggtc tatatagcca
     1741 aaattattgg aaatggagtt aatcaaagtg tcaaatttct caaaatcaac taaataaaca
     1801 ataatttacc ttttaaagag aattaattta tcccggataa tttattttat tccaatcttt
     1861 gtattcagat aaacaaattg taccaacaaa actttgtatt tgaattagaa aaattaaaaa
     1921 acatgaaata tttacattta tacatatttg tctttatttt tcaaatcgct gatgcacaga
     1981 taattacttc gtccactaat cctactcccg ggactgttaa caaacagata ggcgtaattg
     2041 gttttgatac caatgaagtc aaaaagaccg ggaatgctca ggtgtggaat ttcaacaatc
     2101 taacgctcac aggccttaat attaccacct catacagtga tgttgccggc tccccgttta
     2161 tcaatgaatt tccaggtgcc aatattatac aaaagacaag caaccaaccc gatgcattct
     2221 ttaaagccga taactcgggc atgcatttta taggaggcgt aatgtccggc ccgatgtttc
     2281 cgaatggttt ccttgctgca aaatttacac ctgtcattgc ccaatacaat ctaccaatgc
     2341 aatacggtaa tcacagcgct gaagccactt cattctttta tcctattccc aaggaattga
     2401 taccggacag cctgcttaat aatattccat tccaattgga ttccataagg attcgaattg
     2461 aaatcacaag gaagtacgat tgcatcggtt cgggtacaat ccaccttcct ctgggaagct
     2521 actctgtggt acaggttgat ttcagcttca aacccgggca gaaactggaa gtaaaggtgc
     2581 cgttcctggg ttggatagat gtatcccaat ttgtcaatct gggccagttc aatcagttta
     2641 tcccggagac ggagggcatt tatttccttt cacctcaaca cagtggacca atagctgtat
     2701 ttaatagaaa taccatcacc aaaagactgg aaagtggttt gatttcatca cttgctatta
     2761 atgctactaa tgaaaatata caaaagatca catttgcagt atacccaaat cctgccggtg
     2821 agtatatcga aatcagggac ttcaatttaa aatataccga tatcataatc attgattcca
     2881 atggcaaggt tgttaaatca cttaaaacaa caaacgataa acaaataaat gtcaaagact
     2941 tgcacccagg tgtatattat ttgaatatat tcaacaacaa tcgccaaatt ggcttttcca
     3001 aatggattaa aatgtaaatg atatggaagc cctgcaatgg tcctggaagg aattctttac
     3061 ggttttcgta tcgttgtttg ccgtcattga tatacttgga tctattccct tacttatatc
     3121 gttaaaggaa aaaatgggat atatacgatc cttttatgtc gtgcttattt caggcattct
     3181 tatgattctt ttttattttg taggaaggcc ctttcttaac cttttcggct tagatgagca
     3241 ctcatttgct gttggaggcg ccattgttat tttcttgctg gctcttgaga tggtacttgg
     3301 gcgcgatatt tttcacacgg acagcaaccc aaaggccggt acccttgtgc ccattgcctt
     3361 ccctttaatt gccggtgccg gtacacttac taccatcatg tcattgaagg ctacttatga
     3421 aacgagagga aagaatgaat tagtgatcct ggtggctatc ttagccaacc tgatcattgt
     3481 gtatattgta ttgaaatcgc ttaaatgggt gcagcgagtc atcggagtat caggccttat
     3541 agctatcagg aaattctttg gtgtcattct ccttgctatt gccattcaaa tatttgtaaa
     3601 aaatataacc ttgatcggac tctgaaaatt gattaaagct gatataaata tttgtatcat
     3661 ctttttatcc ctttatcaac aatcattctt ctgaaaacgt tactaatgtt caaataacca
     3721 ttaattatta gattaatttt tagttttgcg actttaagaa aaaatcatgt cggtagaggt
     3781 aaacaatctg actaaaatat acggtacaca aaaagccctg gacaatgtaa gtttcagcgt
     3841 ttctgaagga gatatccttg gttttcttgg acctaatgga gccggcaagt ctacaaccat
     3901 gaagatattg tcttgcttta ttccacaaac cagtggtgaa gcgacaatcg gtggaaagaa
     3961 tgtcaaatcc gaatctcttg aagtgagaag acttgtgggc tacctccctg agcataaccc
     4021 cttatataag gatatgtata tcaaggagta cctattgtat attgccaaat tgtatggaat
     4081 tgccaggcct ttcaaccggg ttaaagatat gatcgaatta accgggctgg gtcgggagca
     4141 aaagaaactg attagccaga tttcgaaagg gtatcgccaa cgtgtcggct tagcccaggc
     4201 tatgattcac aatcctgagg tgttaatcat ggatgaacct accagtggac tggatcccaa
     4261 ccagttgatg gagattcgca atgttatcag agaaattgga aaaga
//
LOCUS       DAOQQN010000290         4285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9658_length_4285_cov_5.037352, whole genome shotgun sequence.
ACCESSION   DAOQQN010000290 DAOQQN010000000
VERSION     DAOQQN010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9658_length_4285_cov_5.037352"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..950)
                     /locus_tag="PLG24_10350"
     CDS             complement(150..950)
                     /locus_tag="PLG24_10350"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HMZ25334.1"
                     /translation="MIYEILGYIGAFLMGISLGAIGGGGSIFSVPILVYLFGVNPVIA
                     TGYSLFVVGTTSLSGSLSHIRVGNINWRAVFFFGIPSIASVFITRTLIVPAIPDPVFA
                     GTWIEVGKPSFFLLVFAVFMLAAAFAMTRKKKVEEDNTTNANSLMITIAGLFEGLLTG
                     LVGAGGGFLIIPALVLLGKVEMKKAIGSSLLIIAVKSLMGFTGDLHNNVNIEWKLLIP
                     FTLIAIGGIITGSRLSRRISNEKLKPLFGYFILIMGLIILAKELLIGR"
     gene            complement(947..2083)
                     /locus_tag="PLG24_10355"
     CDS             complement(947..2083)
                     /locus_tag="PLG24_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HMZ25335.1"
                     /translation="MIQTVAVIGSGAMGTGIGQVAATHQCTVIFCDASAESLEHSRHI
                     LTSSIERMVEKVKLTGEAGRDIIGRCRWTSDMKEIAGADLIIEAIVEKFEAKEALFKA
                     ISEIIPDQTIVATNTSSLSITALAATISHPERFIGLHFFNPAPIMRLVEIVPALQTEK
                     TLVEKMQHLMASWAKTAVVARNTPGFIVNRVARPFYGEALRIFDEGLAGCRAIDTAMK
                     EVGGFRMGPFELMDFIGHDVNYRVTESVYNAMFQDPRYKPSLTQKSLLDAGWLGRKTG
                     RGFYAYPDMEETMTSYSSGLNPTEIFGRIFAMLVNEAVDAVYMNICSEEDVENAMQLG
                     TNYPQGLISWGRTFGMDRIMDIMSGLYERYREDRYRPSPLLTNI"
     gene            complement(2173..2952)
                     /gene="paaG"
                     /locus_tag="PLG24_10360"
     CDS             complement(2173..2952)
                     /gene="paaG"
                     /locus_tag="PLG24_10360"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HMZ25336.1"
                     /translation="MNYQYIEFTKDGNIATIRFNRPEVFNSVNKPMAMEIQEALDRCK
                     DEDVRCILITGTGKAFCAGQDLNEVVDPNGPELRSIVKEHYNPIIRRIREIEKPVICA
                     VNGVAAGAGANIALSGDIVVAARSASFVQAFSKIGLIPDSGGTFFLPRLIGWNRASAL
                     MMTGDKVSADDAILMGMLYKVYEDEVFQDKIKALAAQMASMPTYGLALTKKALNASNV
                     NDLNTQLDLEEQLQTSAGGSYDYNEGVVAFLEKRMPVFKGK"
     gene            3388..>4285
                     /locus_tag="PLG24_10365"
     CDS             3388..>4285
                     /locus_tag="PLG24_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25337.1"
                     /translation="MYHNFRNTAQAILMCVLLLTAAVASAQKIEVFGLTKDSLIWKKY
                     VSEMIAADNPDTFALFATCRTIIDTLQAYGYYSAAVDSIKVRDGKTGIYFYQGKKFNV
                     RDLIIMPYMNKQERDTITTPEKDTIPMRERIRKEQRKMIETAILNGFPFAATHLEPII
                     IDSQSVDMKLLLSPGDLYTIGGLHVIGDLKISNFYLEQMLGLRVGAPYYPGLPDKAGK
                     KIRELTFAEQYKSPAVVFAEKKAWLALFLKKKNVNRFDLLIGFQPNTSNIPGVSNKFL
                     LTGNATIELLNQFGKGEKLLGEY"
BASE COUNT         1207 a          996 c          889 g         1193 t
ORIGIN      
        1 taatacgcgc cctgagcgag gcatcccgtg tatatttgtt ctactttcat ctacaatata
       61 ttaatctttc tttaaatatt ctaaaggtcc ttgtttatcc cttatttgaa gtcaatgttc
      121 ctattcaaat tcccttcaat aagtccacct taccttccta tcaataactc ttttgccaga
      181 atgattaacc ccatgatcag tataaaataa ccaaacaggg gtttgagctt ttcgttcgaa
      241 atgcgtctgg ataatcggct gcctgtaata attccgccta tcgctattag cgtaaaaggt
      301 atcaacagtt tccattcaat attcacattg ttatgcagat cgcctgtaaa tcccatcaga
      361 gattttacgg ctataatcag caaacttgat cctatagcct tcttcatttc gaccttaccc
      421 agtaacacca gggcaggaat gatcaaaaat ccaccaccag cacctacaag tcctgtcagc
      481 aggccttcaa acaaaccggc aatggtaatc atcaatgaat tagcatttgt ggtattgtcc
      541 tcttcgactt tcttttttct tgtcatggca aatgccgctg ccaacatgaa tacggcaaag
      601 accaacagga agaaactcgg cttgcctacc tctatccagg ttccggcaaa caccggatcg
      661 ggaatggcgg gtacaataag tgtacgggta ataaagacag atgcaatcga tggaataccg
      721 aaaaaaaata ctgcccgcca gttgatattg ccgacacgta tatggctcaa tgagccggat
      781 aaacttgttg tccctacaac aaacaaggaa tagcctgtgg caataaccgg gttgacacca
      841 aataaatata ccaggatggg cacagaaaat atcgaaccgc caccgccaat ggctcctagt
      901 gatattccca tgaggaaggc tcctatatat cctaatattt cgtatatcat atattggtca
      961 aaagggggct gggtctgtaa cgatcttccc tgtacctctc atagaggccg gacatgatat
     1021 ccatgatgcg gtccatcccg aatgtcctgc cccaactgat caatccctgt ggataattgg
     1081 tgccaagttg cattgcattt tctacatcct cctccgagca gatattcata tatactgcat
     1141 cgacggcttc gttgacgagc atggcaaaaa tacggccaaa tatttcagtg ggattcaacc
     1201 cggatgaata tgaggtcatg gtttcttcca tgtcaggata ggcataaaag cctcttccgg
     1261 tctttctacc caaccagcct gcatccaaaa gggatttttg cgtcagagac ggtttgtacc
     1321 ttggatcctg aaacatcgca ttatatacgg attccgttac ccgatagttg acatcgtgcc
     1381 ctatgaagtc catcaattca aaaggtccca tacggaagcc gccgacctcc ttcatggcgg
     1441 tatcgatagc tctgcaaccg gcaagccctt cgtcgaaaat gcgtaaagcc tcaccataga
     1501 acggcctggc caccctattg acaatgaagc ccggtgtatt gcgggcaaca acagctgtct
     1561 tggcccaaga agccatgagg tgctgcattt tttcaaccaa ggtcttttcg gtctgtaatg
     1621 caggcacaat ctcgaccaac ctcatgatag gggccggatt gaagaaatga agtccgataa
     1681 aacgctcagg atggcttatc gtagcagcca gggcagtgat gctcagggac gaagtgttgg
     1741 tcgccacaat ggtttggtcg ggaatgattt cagaaatggc cttgaacaat gcttcctttg
     1801 cttcgaattt ttcaacaatc gcttcaatta tcaggtcggc acctgctatc tctttcatat
     1861 cacttgtcca gcggcatctt ccgatgatgt cacgaccggc ttcaccagtg agctttactt
     1921 tttcaaccat tctttcaatg gatgatgtca ggatatggcg actatgctca agtgattcag
     1981 ctgatgcgtc gcaaaaaatc accgtacatt gatgcgtcgc agcaacctga cctatgcctg
     2041 ttcccatggc gcctgaacct ataactgcaa cggtttggat cattgtgttt taatgataaa
     2101 gggattgaaa agattggttt ttagcatgcc aatgctcaaa tcctgactta aacaaagcga
     2161 tggattgaaa gtttattttc ctttaaatac aggcattctt ttttcgagaa atgccactac
     2221 tccttcatta tagtcataag aacctccagc acttgtctgt agttgctcct ccagatccaa
     2281 ttgagtattg aggtcattga cattggaagc attgagcgcc tttttggtaa gagccaggcc
     2341 atatgttggc atggaagcca tttgtgcagc caatgctttt atcttatcct gaaatacttc
     2401 atcttcatat accttgtaca acatacccat gaggatagca tcatcggcag aaaccttgtc
     2461 gccggtcatc atcagtgcag atgctctatt ccatccgatg aggcggggca gaaaaaatgt
     2521 tccgccacta tccggtatca ggcctatctt cgagaatgct tgcacaaatg aggctgatcg
     2581 ggcagccact acgatatctc cactcaaggc tatattcgct ccggcaccgg cagcgactcc
     2641 gtttaccgca caaataacgg gcttttcgat ctccctgatt cgcctgatga tggggttgta
     2701 atgctccttt acaatactcc gaagttccgg accattggga tccacaactt cattcaaatc
     2761 ctgtcctgca cagaacgcct tacctgtacc cgtaatgagg atacaccgca catcttcatc
     2821 cttgcatcgg tccagcgcct cctgtatctc cattgccatt ggtttattaa ccgaattgaa
     2881 aacctccggc ctgttgaaac ggatggtggc tatatttcca tccttagtaa attcaatata
     2941 ttgataattc atgaaggaat agtttgtggc aaacctacaa aaaattgggc aactattcat
     3001 ttcagccggg aaggaatgac tctgcttttg accttcagaa atatggaaaa tacagtgatt
     3061 ggatacctgg aggttacaat acggtgggga cagaaaaaat cgaagtgtgt acttgtttaa
     3121 tagctgaaaa ctaagtgtag tcgggcatct tcaagggagt aatttatttt ttgtctcaag
     3181 atacaggtat taattttttt cattttccca ttcttcaatc catcagtggc tttgacttat
     3241 tactttccta cctacttcga ttttgaccgt ttgccatgac cttggcaaat atttatatca
     3301 tcccataaag actaaatata agtatcttcg ttaatgtaaa tggtgactct aagcagacat
     3361 aatatccgac cgatcactga tacaaaaatg taccacaatt tccgaaatac cgctcaggct
     3421 atattaatgt gcgttttatt gctgacggcc gcagttgctt ccgcacagaa aatcgaggtg
     3481 ttcggattga caaaggatag cttgatctgg aaaaaatatg tgtcagagat gattgcagct
     3541 gacaatccgg atacctttgc gttatttgct acatgccgga ctatcatcga caccctgcag
     3601 gcctatggtt attattctgc agctgtcgat tcaataaagg tacgggatgg aaagaccggt
     3661 atctatttct atcagggcaa gaaattcaat gttcgggacc tgatcatcat gccatacatg
     3721 aataagcagg agagggacac cataacaacg cctgagaagg acaccatacc tatgagggag
     3781 cggataagga aggaacaaag aaaaatgatc gaaacggcta tcctcaacgg gtttccattc
     3841 gctgctactc acttagagcc cattatcatt gacagccaat cggtcgatat gaagttgttg
     3901 ttgtcaccgg gagatcttta taccattggt gggctacatg ttatagggga tttgaaaata
     3961 tcgaactttt accttgagca aatgctggga ctcagggtag gcgcacctta ctatcccgga
     4021 ttgcctgaca aagccggtaa gaagatacgg gaactgacct ttgccgagca gtacaaaagc
     4081 cccgctgttg tatttgcaga gaaaaaggct tggcttgctt tattcctgaa gaaaaaaaat
     4141 gtcaaccgat tcgacttgtt gattggtttc cagcctaata ccagcaatat tcccggagta
     4201 agcaacaaat ttctgctcac aggaaatgca accatcgagt tgctcaatca attcggcaag
     4261 ggagaaaaat tattgggtga atatt
//
LOCUS       DAOQQN010000291         4265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9726_length_4265_cov_5.813539, whole genome shotgun sequence.
ACCESSION   DAOQQN010000291 DAOQQN010000000
VERSION     DAOQQN010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9726_length_4265_cov_5.813539"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PLG24_10370"
     CDS             <1..183
                     /locus_tag="PLG24_10370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ25338.1"
                     /translation="NDLNVNVSIIDEKGQSVRSEIIHSGKLASTGIDMSSLNPGSYIV
                     KIISDYSNSILRVIKE"
     gene            822..1841
                     /locus_tag="PLG24_10375"
     CDS             822..1841
                     /locus_tag="PLG24_10375"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HMZ25339.1"
                     /translation="MDHFDEYLRQGEPNKAEKAKNWKTAIGLQQVDGLKPSDYLIETA
                     RQNIEGDITLDEVKKRIDSYYQQHPTKRDEDRTEEADKVSARIAEMLSEQTFTFSPVE
                     YLAIHRRLFQGIYKFAGKIREYNITKKEWVLNGDTVFYASADSLKDTLEYDFEQERKF
                     GYKGLNEQEILEHIAHFISGLWQIHIFGEGNTRTTAIFLIKYLRKLGFKNVNNDLFSE
                     HSLYFRNALVRANYEDLSQGIHKTEEFLIRFLSNMLLKENHSLKNREMHIHYSDTVKS
                     RNDTVNDTVFSLIKQNNRITANEICERLKMSLSTVRRKIKVLKEENKIERIGSDKTGH
                     WRITE"
     gene            complement(2277..2657)
                     /locus_tag="PLG24_10380"
     CDS             complement(2277..2657)
                     /locus_tag="PLG24_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25340.1"
                     /translation="MKSALKIFILIFLIVFGLLTLFMSISVTFDLFGMRAGHEHFVPF
                     VVYANMFCGLLYVLSAYKLINKQSGARKLLLLAIGILILTLIAFSIHVAKGGEYDQNT
                     FYALAFRTGVTALLAWMSKWYIHR"
     gene            complement(2772..3359)
                     /locus_tag="PLG24_10385"
     CDS             complement(2772..3359)
                     /locus_tag="PLG24_10385"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMZ25341.1"
                     /translation="MKKTTRRTLIEYGIFILIALVLYLTGKHTEVVGFLQRRLLATGI
                     MNASVDNPAKGVESTSPMADLAMTLIDSKGKQVNLETFRGKVIFLNIWATLCPPCIAE
                     MPSIHELYKKLGNDVQFVMISVDNDFETAKRFRERKEYSFEIYSLVSSMPTMYQTKSI
                     PSTFVIDTEGRLVYQHQGMADYNTDPFIQFLRELK"
     gene            complement(3978..>4265)
                     /locus_tag="PLG24_10390"
     CDS             complement(3978..>4265)
                     /locus_tag="PLG24_10390"
                     /inference="COORDINATES: protein motif:HMM:NF027650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter C-terminal domain-containing
                     protein"
                     /protein_id="HMZ25342.1"
                     /translation="LELQEQQERDKKASAPIVQVKSAAAISYEDQKEIQRLERDIRKL
                     EEERNAVTEKFNDLSLTVEDIQKHSERLKKIEEMLAEKEMAWMELMEKYEN"
BASE COUNT         1340 a          806 c          817 g         1302 t
ORIGIN      
        1 aatgatttga atgtcaatgt ttctataata gatgagaaag gtcaaagtgt gcggagcgaa
       61 attattcatt caggaaaatt ggcttctaca gggattgata tgtcaagtct taacccgggc
      121 tcatatattg taaagattat ttctgattac agtaattcta ttttacgcgt tattaaagaa
      181 taatgtcgtc gtattttttg aaaaaggaga taacctcggt tatctccttt ttttcatatg
      241 ctcttaatag aggcaaaaga ctaataaata tgtaagcaac tttctggcta catttttttc
      301 aaatcttcaa tggtcatacc ttggcggaaa aataaaaacc atgacaaagt acgagattgc
      361 ggaacaatat gcgaagtcag ggtggggaac atgcgggatt aaattgcagt ccggattttt
      421 gtaaaaagaa tgaaagaatg agggcaagca gaacgccgat gatcccttac tcagcccgca
      481 taaattgggc tgatatgaat gtaaatgcgg cattacatgg gtcaaccggc aacatctaag
      541 attatatgtt atgtgcccga tgggtcgaga gggtcacaca cggttgtgtg agaggcttaa
      601 actgaaaatc ttttgactgc ttgattccga aaacgttggt ggcaatcccc aaaacgattg
      661 tattaaaatt caactgacaa caaaaaaatg taactttgtt cctgatgata tgagaatatg
      721 gaattaacaa aaaacgctta accgaacttc agagacggct gaaaattgga agacttgaca
      781 actttgtaga gatattaaaa attgataact aatagaaagt aatggatcac tttgatgaat
      841 atttaaggca aggcgaacca aataaggcgg aaaaggcaaa aaattggaaa acagctatcg
      901 gtttacaaca agtggacgga ctaaagccgt ctgattatct tattgaaact gccagacaaa
      961 acattgaagg tgacataacg cttgacgagg taaaaaaacg tattgacagt tactaccagc
     1021 aacacccgac taaaagagac gaagacagaa ccgaagaagc ggacaaagtt tctgcacgca
     1081 ttgccgaaat gttgagcgaa caaacattta cattttcgcc ggtagaatat ttggcaattc
     1141 atcgaagatt atttcagggt atatacaaat ttgccgggaa aattcgtgaa tacaacatca
     1201 ctaaaaaaga atgggtactg aatggagaca ctgtatttta cgcaagtgct gacagcttaa
     1261 aagatacgtt ggagtatgat tttgaacaag aaagaaaatt tggctacaaa ggattaaacg
     1321 aacaggaaat acttgaacat atagctcatt ttatttccgg cctgtggcaa atccacattt
     1381 ttggagaagg caacacaaga acaacagcca ttttcttaat caagtatctg cgaaaacttg
     1441 gttttaaaaa cgtaaacaac gatttgtttt cagaacactc cctgtatttc cggaatgctt
     1501 tggtaagagc aaactacgag gatttatcac aaggtattca caagacagaa gagtttttga
     1561 ttcgttttct ctcaaacatg ttgcttaaag aaaatcattc tcttaaaaac agagaaatgc
     1621 acattcatta ttctgacact gtaaaatctc ggaatgacac tgtaaatgac actgtatttt
     1681 cattgattaa gcagaacaac agaataacag ctaatgaaat ctgcgaacgc ttaaaaatga
     1741 gtttaagtac cgtaagacga aaaattaaag ttctcaaaga agaaaataag atagaacgca
     1801 ttggtagtga taaaacagga cattggagaa taaccgaata ataatcggaa tgtaagtacc
     1861 tctaaataca tttctttcca agtcatcaat ggtcatgcct tggcggaaaa ataaaaacca
     1921 tgacagattt cctcgattgt cagatgcgta ctataggtgt tgaatatcat atcgctctga
     1981 ttgtgttgat tgttgggagc aacacctcat ttcatctaaa tcattcatga agaagtttca
     2041 attaattgtc aagtcaggta cttagaaagg gtaaagtctt agctaccata accggtccta
     2101 aaatgatttt ttaacgcgtg gtattaattt atcatcctga ttaaaataca aagtatacga
     2161 ggctgtgcgt attaacgtaa aatatgataa ttggtaaagc ttcaaaattg caaattctta
     2221 tgcaacagat ggccggtatt gcctgagttc ccgatgcatt caagatttca tttatgctat
     2281 ctgtggatat accatttact catccaggcc aataaggctg taacacctgt acgaaaggca
     2341 agggcataaa aggtattttg atcgtattcg cctcctttag ccacatgaat cgaaaaggca
     2401 ataagggtca ggatcagaat gccaatagca agtagaagaa gtttacgtgc acctgattgc
     2461 ttattgatca atttatatgc cgataataca tataacaggc cacaaaacat gttggcataa
     2521 accacaaatg gtacaaaatg ctcatgcccc gcccgcatac cgaacaaatc aaatgtcacc
     2581 gaaatggaca tgaataatgt aagcagtcca aagactatca ggaagatcaa gatgaagatt
     2641 ttcaatgcgc ttttcataat gtatgagttt tcaggttgac gaaatgagca tttctttaaa
     2701 atgaattctg tgtttgactt cttttttcta caatgtagta attctaaacc ttccttatca
     2761 aatttatccg atcatttcaa ctcccgaaga aattgaatga atgggtctgt attgtaatcg
     2821 gccatgccct gatgctgata caccaacctg ccttcggtat cgattacaaa ggtcgatgga
     2881 atgcttttag tttggtacat ggtcggcatg ctggaaacaa gcgaatatat ttcaaacgaa
     2941 tattcttttc tttccctgaa tcttttggct gtttcaaagt cattatccac ggaaatcatg
     3001 acaaattgga catcattgcc taattttttg tacaattcgt ggatggatgg catttcagcg
     3061 atgcatggtg ggcacaaggt ggcccagata tttaggaaga ttaccttgcc gcggaatgtt
     3121 tccaggttga cctgtttgcc ttttgaatca atcagtgtca ttgcaagatc cgccattggc
     3181 gacgtcgatt ctacaccttt cgcaggatta tctacggatg cattcatgat gccggtagcc
     3241 aacaacctcc tttgtaaaaa ccctactacc tctgtatgct ttccggtcag ataaagtact
     3301 aaggctatca ggatgaatat tccgtattct attagggtcc tgcgtgtggt ctttttcatg
     3361 gatattttat ttcattcttt tttggattgc atccccatct gccatgttcc atttctattt
     3421 gtaataaacc gggacaatca tcacctgact gcaatcttat gcacaacatc aaatttccgg
     3481 atcaggtttt gtatctttcc ttcgatgcct gatttgcatt attttgattg tataacaatc
     3541 cacaccctaa acgactttcg attccggttt tattcaatgt tgtgcttttt ttccggattg
     3601 gagaaattgt tgtgttcgtc aaatttgttt tttgcctgaa tccagaattt cacagactac
     3661 cggattattc gatttattga aacttaatat ttcccaagcc acctattcct gtactcattc
     3721 gttttttgag gtagatctat acattcctaa ccatagcact ttttaccggt tataagttta
     3781 attgtgatcc gaacgttggc tctccgaagc ttttttatgc agatgaagat tacttccact
     3841 acggatatcg tccccggacc tcatcggaat ctattgggcc attgtaatac cattcatagg
     3901 agatgtaact gtttactgag actcattggt cgtaatgctg ctttgatcca tgaacattca
     3961 cacatagcca ttcatctcta attttcatac ttctccatga gttccatcca ggccatttcc
     4021 ttttccgcca gcatttcttc aatttttttc agtcgctcgg aatgcttttg tatatcttcc
     4081 actgtaagag acaagtcgtt gaacttctct gtaactgcat tccgttcttc ttccagcttt
     4141 cggatgtctc gctccagccg ttgtatttcc ttctgatcct catagctgat agctgccgct
     4201 gacttgacct ggacaatagg cgcagaagct ttcttgtcgc gttcctgttg ttcctgaagt
     4261 tcaag
//
LOCUS       DAOQQN010000292         4256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9763_length_4256_cov_6.003809, whole genome shotgun sequence.
ACCESSION   DAOQQN010000292 DAOQQN010000000
VERSION     DAOQQN010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9763_length_4256_cov_6.003809"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /gene="queG"
                     /locus_tag="PLG24_10395"
     CDS             complement(<1..660)
                     /gene="queG"
                     /locus_tag="PLG24_10395"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HMZ25343.1"
                     /translation="MELLYNPSENNTMGEMDDVRGYITGLALDEGFTAVGFSRSEYLE
                     KDEHYLQDWLLKGSHGAMSYLERNLDLRTDPSILNPGTRTVISLIYNYNQDNPTRDKS
                     LKISTYALGRDYHKVIRKKLKNISRKILENVAPEANIRYFTDSGPVLEKEWARRAGLG
                     WIGKNTLLIHPKRGSYFFLAEILIDLDIAVDDTLAIRDHCGTCTRCIEACPTDAISPD
                     GY"
     gene            complement(780..1361)
                     /locus_tag="PLG24_10400"
     CDS             complement(780..1361)
                     /locus_tag="PLG24_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25344.1"
                     /translation="MKNLFILIFIAASMSVQGQVRSMFDAPSIYLTSRDITHALNQEG
                     LGLDFGYGLGTHWMFAKLSVGVHALADFDAPKIDKSFFANPFGRFELGLGKWRSNGKQ
                     CAITHQNAYSYLVKGGIMYNMGKRKADVENKISEITSGIDYFIVAELGLFFIKDMFRN
                     SEYYINGGYMLNSKTVFAELGLRTFFNTRYAGR"
     gene            1884..2162
                     /locus_tag="PLG24_10405"
     CDS             1884..2162
                     /locus_tag="PLG24_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25345.1"
                     /translation="MALILKLFTRFRPGNIILHNNRQHYCFTIFNAFPNIRRLSRSGS
                     WFYEVTLLIIHKIFIRGISFVGKLTIAKSYFAISKSNVILPWNNWPKH"
     gene            2250..2843
                     /locus_tag="PLG24_10410"
     CDS             2250..2843
                     /locus_tag="PLG24_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ25346.1"
                     /translation="MSAKHLSKKEDIFENELLPHVDALTTFAFHLTYNEEDARDLVQE
                     TFLKAYKSIDSYAIGTNSKAWLFKILKNAYINEYRKKSKRPTEVDFEDFLAYLEKEDL
                     TIKGSQDFKLDMYQNLIGDEVTIAINSLPMDFKTVILLCDIEGFTYEEISKITDIPIG
                     TVRSRLHRGRKMLRDKLRQYALKLGYKDSKSSGKKEK"
     gene            2877..3107
                     /locus_tag="PLG24_10415"
     CDS             2877..3107
                     /locus_tag="PLG24_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25347.1"
                     /translation="MNSKVDQNRNHFIHHHIESNPEPNTELSHFNNELNNLFLGHKLP
                     QSSFKEFLKSKVHRPQVSTEFIEKIRNKITGD"
     gene            3285..>4256
                     /gene="prfA"
                     /locus_tag="PLG24_10420"
     CDS             3285..>4256
                     /gene="prfA"
                     /locus_tag="PLG24_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HMZ25348.1"
                     /translation="MLDKLTAIEDKYHLLEEQLSDPAIISDLSRFKKVNKEYKDLKEI
                     IASFKVYKQILSGIETAREMIADPDPEMQEMGQSEIEGLEQEKTRKEDQLRLMLIPKD
                     PEDSKDVLFEIRSGTGGDEASIFAGDLFRMYTRYFDSQGWTYEILEANEGDSGGYSKI
                     ILEVSGEDVYGKMKFESGGHRVQRVPKTESQGRVHTSAATVAVIPKLEEEDVDIKKSD
                     LKVDTFRASGAGGQHVNKTESGVRFTHIPTGIITESTEARSQIKNREIALQRLYQLIM
                     EKNRNEVQNAMASQRKTQVGTGDRSDKIRTYNFPQNRLTDHRINLTLY"
BASE COUNT         1423 a          915 c          798 g         1120 t
ORIGIN      
        1 gtacccgtcc ggactaattg catcagtcgg gcaggcttca atgcatctgg tacaggtacc
       61 acaatgatcc ctaatggcca aagtgtcgtc caccgctatg tccaggtcaa tcagaatctc
      121 agcaaggaaa aagtaagaac ctcgctttgg atgtatcaac agcgtattct ttccaatcca
      181 gccaaggcct gcccttcttg cccattcctt ctccagcacc ggtcctgaat ccgtgaaata
      241 ccttatatta gcttcgggag ccacattctc caatatctta cgggaaatgt tcttcagttt
      301 tttcctgatg accttgtggt agtccctgcc cagcgcatat gtcgaaatct tcaggctctt
      361 atcccgtgta ggattatcct gattataatt gtaaatcaag ctgatcacgg tacgggtacc
      421 tggattcaga atagagggat ctgtccttag gtcgaggttt ctttcaagat aagacattgc
      481 tccatggctt cccttcaaaa gccagtcctg cagatagtgc tcgtctttct ccaaatactc
      541 agacctggaa aagcccacag cagtaaagcc ttcatcaagt gccaaccccg taatatagcc
      601 gcgtacatca tccatctcac ccatcgtatt attttccgaa ggattgtata acaactccaa
      661 aactagccca aatatcggtt tttatacagg tcaattcaaa aaaatgggta aaaagagatc
      721 aaaatcccct tttacccatt tggtttgagt acttgatttc ttacaagtga gcacaagtct
      781 tacctcccgg cataccgtgt gttgaagaaa gtcctcaagc caagctcagc aaagactgtt
      841 ttactgttta gcatataacc accattgata taatattcag aatttctaaa catatccttg
      901 atgaaaaaca aacccaactc agctacgatg aagtaatcta taccggatgt aatttcactg
      961 attttattct cgacatctgc tttccgcttc cccatattat acattatacc acctttaaca
     1021 aggtaggaat aggcattctg gtgtgtgata gcgcattgct taccgttgga gcgccactta
     1081 cctaatccga gttcaaacct accaaaagga ttggcaaaga atgatttatc gattttaggc
     1141 gcatcaaaat ctgccaatgc atgcactcca accgataact tagcaaacat ccaatgtgta
     1201 cccagcccat aaccaaaatc aagtcccaaa ccctcctgat tcagtgcatg agtaatgtct
     1261 ctggatgtca aataaatgga tggtgcgtca aacatagacc taacctggcc ttgaacactc
     1321 atgctggccg caatgaagat taaaatgaat aggtttttca tgattaaaaa ttttaaatgt
     1381 tgactaaatt tctgcaaata cagtgtacaa atttccgcaa ttcattattt cggaataaat
     1441 ataaaataaa aattataacc ccgaaggtca cattttgcac tcaaagataa aaataccctc
     1501 gtattcttca tacggacaag atcccaacat cttgaatcca acaaaatttc caatcaaaac
     1561 cagcatcttt cattctttcg attaaatctt actttgtcaa tcgaaattcc ggagtaaaat
     1621 agccacctga aagcaagaaa ttccgaatgt attgcctcac ttgtgtgaga aagggacgaa
     1681 aagtaatctt atttattttt gagatgaatc actaccttgt aaagtgtaca aaaggaaagc
     1741 ttgaaattac acacatccat aaccagaagc cgtggaaaac cgacataaat acatccggct
     1801 atccgaataa attgcatgat aaaatttgtc cccgatactg cggaaaatga atctctcagc
     1861 gaaaatcaaa gcatggatga gagatggcgc tcatactaaa actattcacc cgatttcgtc
     1921 ccggaaatat tatcctacac aataatcgac aacactattg cttcactatt ttcaacgcat
     1981 ttccaaacat ccggcgctta agtagatcag gaagctggtt ttatgaggta acactactca
     2041 tcatccataa aatttttatc agagggatat cctttgtggg caaactgaca atagccaaat
     2101 catactttgc catttccaaa tccaatgtaa tcctgccatg gaataattgg cctaaacatt
     2161 gaaagttgtt tgataaaaag atacattatt ccattgttat cttaatttta agaaaaaaat
     2221 tgcacttttg catctgatta atcatcaata tgtctgcaaa acatttgtcg aaaaaagaag
     2281 atatcttcga gaacgaactc ttgccccatg tggatgctct aacaaccttt gcattccatc
     2341 ttacctataa tgaagaggat gcaagggatt tggtacagga gactttttta aaggcatata
     2401 aatcaatcga cagctatgct attggaacta attctaaagc ctggctgttt aaaatattga
     2461 aaaacgctta tatcaacgaa tacagaaaga agagtaagcg tccgacagaa gtcgattttg
     2521 aagactttct ggcatatctc gagaaagaag acctgaccat caaaggaagt caggatttca
     2581 aattggatat gtatcaaaat cttatcggcg atgaagttac tattgctatc aactcattgc
     2641 caatggattt taaaacggtt atattgctgt gcgatatcga gggatttacc tatgaagaga
     2701 tatccaagat tactgatatt ccgattggaa ctgtcaggtc aagactgcac agaggccgca
     2761 aaatgttgcg cgataaacta agacaatatg ccctaaaact tggttataaa gactccaaat
     2821 catccggtaa aaaggaaaaa taagcattat aaatattgca aacaggaatt ataatcatga
     2881 atagtaaagt agatcaaaat cgaaatcatt tcattcacca tcatattgaa tcaaatccgg
     2941 agcccaacac agaattatcc cacttcaaca atgaattaaa caacctcttc cttggacata
     3001 agctacctca aagttccttc aaggagttcc ttaaaagcaa agttcatcgt ccgcaagttt
     3061 ctacagagtt tatcgaaaag attcgtaata aaatcaccgg cgactaaaaa gcttcaaaag
     3121 cctgaaatct tctgacccta ctcagtaaaa aagtgtgccc ctgcaatata aatcattgga
     3181 ggttaaattc aaattcaggt attttatgaa aggggaatct acagttcagg gataaatttc
     3241 acattgttgc gtacttttgc agccattttc ataaaatccg aatgatgctg gacaaactta
     3301 ctgctataga agataaatat caccttctgg aagaacaact ctccgatccg gcgataatca
     3361 gtgatttgtc gcgctttaaa aaggtaaata aggaatataa ggacctgaag gagattatag
     3421 catctttcaa ggtctacaag caaatactca gtggtataga gaccgcccgg gagatgatcg
     3481 ccgaccctga tcctgagatg caggaaatgg gccaaagtga aatagaaggc cttgaacagg
     3541 aaaagacaag gaaagaagat caactcaggc tgatgctcat accgaaggat cctgaggact
     3601 caaaggatgt gctttttgaa ataaggagtg gtaccggtgg cgacgaagca tccatcttcg
     3661 cgggtgactt attcaggatg tacacccggt atttcgacag tcagggatgg acctatgaaa
     3721 ttctcgaagc caatgaaggc gactcaggag gatacagcaa aataatcctc gaagtgagcg
     3781 gcgaggatgt atatggaaaa atgaaattcg aatccggtgg tcatagggtg cagagagtgc
     3841 ccaaaacgga atctcaaggt agggtgcata catcggctgc cacggtggct gtcatcccaa
     3901 aactggaaga agaagatgtc gatattaaaa aatcagacct caaggtagat accttcagag
     3961 ccagcggagc aggtggacag cacgtcaaca aaacagaatc cggcgttcga tttacccata
     4021 ttcctaccgg catcattacc gaaagtaccg aagctcgctc acagatcaaa aacagggaga
     4081 tcgccttgca acgactgtat cagctgatca tggagaagaa cagaaacgag gtacagaacg
     4141 ccatggcttc acagcgtaaa acccaggtag gaacaggtga caggagcgat aagattcgaa
     4201 cttataattt tcctcaaaat agacttacag atcatcggat caatctaacc ctttac
//
LOCUS       DAOQQN010000293         4243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9805_length_4243_cov_4.962273, whole genome shotgun sequence.
ACCESSION   DAOQQN010000293 DAOQQN010000000
VERSION     DAOQQN010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9805_length_4243_cov_4.962273"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2532
                     /locus_tag="PLG24_10425"
     CDS             <1..2532
                     /locus_tag="PLG24_10425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HMZ25349.1"
                     /translation="GEPGRSPVVRVRGVSSAIAGTATLYVVDGVLTEDISNINTNDIV
                     SMDILKDASATAIYGARGANGVIIITTKKGASGTMTVNYSSTVGFKNAANLVRMANSA
                     EYANYVSQATSGNVEDPGFSTDWYSEILRNAWYQSHSISLSGGTDKARYYISGGYLED
                     EGIVIDNKYKRFDVRANLNWDLNKWVSAGLISSFANSNNKIANLGTAYNNAYRAAPII
                     KGKEDGRYGNTSVYQNVGNPILDIENNYNREKTNRLLSTGYLDIKPVSWLTLRSSIGA
                     ELSNKGSNVYNYQFENDETKFLNAGGNQRNPNSNLFAQKENALRWVWDNTATFLKKFD
                     NHTVTLLAGTTAEKFTSDFVQAFRKDVPANKNLWYIGTGDANTSTNGGGGDQWARNAY
                     LARLNYNYADKYLFTGTMRYDGSSRLSPKNRWGLFPSFGVGWMLSSESFMANQVLIDV
                     LKLRASWGRVGNDRVPTDAFVTTVEANQNYPFGGGLAIPGSAVTQIKDPNLKWETTEE
                     LDLALEFGILKNRLTGEIGYYNKKARDLLINIKVPSVVGDADGVVLTNAASIRNSGLE
                     MAMNWRTRISDKLSYFAGANITFNNNKVIGLNGGQPILDGGIGAAQQYTTKTDNDQEV
                     GSFYVYQVLGVFQSDEEVLAYTGNTGLPIQPAASAGDFKYLDANNDGVIDDNDRVFVG
                     SYQPDFYYGINLGVTIGRFDLTLDMTGVAGNKVYNGKKAFRQLASDNIERDMAYNRWT
                     RGSGINDEPAANSGNLPASTYFVEPGDYIRINNITLSYTLPEIPGARKIYSNARIYLT
                     GQNVFTFKKYSGFTAELPGDPTSSGIELNAYPTSRTIAAGINFTF"
     gene            2554..4092
                     /locus_tag="PLG24_10430"
     CDS             2554..4092
                     /locus_tag="PLG24_10430"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HMZ25350.1"
                     /translation="MMKNINRLYQAGILVLTLILSTGCSKDFLEVPIQGQATPDSDPA
                     IAEKLVTGVYNSMLAGSAFGGIGDVHGISFITATIIMTDDAEKGSTASDQPNIAAIDN
                     FEHTSTNNFVAALWTGNYNAIAKANQALKALDVAPIADAIKTQYQGEVRFLRAYFYFN
                     LVRYFGGVPKVLQIPADANEANSNPVYQTRASVDEIYSLIREDLNYAIEHLKPRSQSQ
                     VGRANKGAAQAMLAKVELTTRNWQNAYNLCQEVINSGQYSLLAKYEDIWRQVGDNSVE
                     SIFEVQTGQFNNSDFGVEGYCVWQGPRVGGKGGWRDLGFGFNNPTVDLVNEYEPGDIR
                     KDATIIFIDNSGTHKGTILFDGYRIPSQDSVQNARYNYKAYHSENKEIESFFGNRDRK
                     AKNIHLLRYGDLLLMAAESANELGNTGEAATYLNMIRERATLSATTASSQADMREAIY
                     HERRVEMAMEHDRWFDLVRTGRAAKVMKPLKPSFTEGKNELLPIPALQIALSGGKLLQ
                     NPGY"
BASE COUNT         1258 a          827 c         1052 g         1106 t
ORIGIN      
        1 ggtgagccgg gtcgtagccc ggttgtaagg gtcagaggtg taagttctgc aattgcgggt
       61 acggcgaccc tatatgtggt ggatggggtc ctgactgaag acataagtaa tatcaatacc
      121 aatgacattg taagcatgga tatcctcaag gatgcttcgg caactgcaat atacggggcc
      181 agaggagcca acggtgtcat tatcatcacg accaagaagg gcgcgagtgg caccatgact
      241 gtcaattaca gctctacggt gggcttcaag aatgccgcaa atttggttag gatggccaac
      301 agtgccgaat atgccaatta tgtttctcag gctacatcgg gcaatgtaga agatccgggc
      361 ttttcaaccg attggtacag cgagatactc agaaacgcct ggtatcaaag tcacagcatt
      421 tccttgtctg gaggtactga taaggcaaga tattatatca gtggcggtta tctggaggat
      481 gaaggtattg tgatagataa taagtataag cgttttgatg tgagagccaa tctgaattgg
      541 gacctgaaca aatgggttag tgcaggactg atttcttcat ttgccaacag caataacaaa
      601 atcgcaaatc ttggtacggc ttacaacaat gcttatcgtg cagcaccgat catcaaaggc
      661 aaggaagatg gacgatatgg caatacctca gtatatcaga atgtcggtaa tccgatcctt
      721 gatatcgaaa acaattacaa tcgggagaaa accaataggc tactatcaac aggctacctc
      781 gatatcaagc ctgtgagctg gcttaccctt agaagtagca ttggggctga gctttcgaac
      841 aaaggaagca atgtgtataa ttaccaattc gagaatgatg agacaaaatt tctcaatgca
      901 ggaggtaatc agcgtaaccc caattccaat ctctttgctc aaaaggagaa tgcattacgg
      961 tgggtatggg ataatacagc aacctttttg aagaagtttg acaatcatac ggtaacattg
     1021 ttggcaggca ccacagcaga aaagtttact tctgattttg tacaggcgtt cagaaaagat
     1081 gttcccgcga ataagaatct atggtatatc gggaccgggg atgccaatac atccaccaat
     1141 ggtggtggtg gcgaccaatg ggcaagaaat gcctatcttg cccgattgaa ctataactat
     1201 gccgataaat atctctttac cggtacaatg cgatatgacg gcagttccag gttgtcgccc
     1261 aagaatcgtt ggggactctt tccatctttc ggcgtcggtt ggatgcttag cagtgagtca
     1321 tttatggcca atcaagtgtt gatcgatgtg ttaaagttga gagctagctg gggcagggta
     1381 ggcaatgaca gggtgcccac ggatgcattt gtgacgaccg tcgaggctaa ccagaattac
     1441 ccttttggtg gaggattagc aattcccggc agcgctgtaa cccaaatcaa ggatcccaat
     1501 ctgaaatggg agacaactga ggaattggat ctggcactgg aatttggtat tctgaagaac
     1561 aggcttaccg gagagattgg atattacaat aagaaggcca gggatcttct aatcaatatc
     1621 aaagtgccct cagttgtggg ggatgccgat ggtgtagtcc tcaccaatgc tgcatctatc
     1681 cgaaacagtg gacttgaaat ggcaatgaac tggaggacca gaatatctga taagttgtcc
     1741 tacttcgcag gagcgaacat aacatttaat aacaataagg tcatcgggtt gaatggcggt
     1801 caacccattc tggatggtgg catcggagct gctcagcaat acacgaccaa aacagataat
     1861 gatcaggaag tgggctcttt ctatgtatat caagtattgg gtgtgtttca aagcgatgag
     1921 gaggtgttgg cctatacagg gaatacaggt ctacctatcc agccggcagc ttcagcagga
     1981 gatttcaaat atctggatgc caacaatgat ggagtcattg atgacaatga tcgggtattt
     2041 gttggatcct accaaccgga tttttattat ggaatcaatc tgggtgtgac gattgggcgt
     2101 tttgatctaa cgctggacat gacaggagtg gccggcaaca aggtgtataa tggaaagaag
     2161 gctttccggc agttagcttc agataatatc gaacgggata tggcatataa ccgttggaca
     2221 agaggcagcg gcatcaacga tgagccggcc gccaattcgg gcaatctgcc ggcatccacc
     2281 tattttgttg agccgggtga ttatataagg atcaacaata ttactttgtc ttatacactg
     2341 cctgaaatac ccggagcaag aaaaatttac agtaatgcac gaatctacct aaccggacag
     2401 aatgttttta cgtttaaaaa atatagtggt tttactgcag aacttccggg tgatccaacc
     2461 agctcaggaa ttgaactgaa tgcttatccg acatcccgaa ccattgcagc gggcatcaat
     2521 ttcacttttt aatgaattct aatgttgaaa gccatgatga aaaatattaa cagactatat
     2581 caagctggaa tattggtgct gacccttatc ctttcgaccg ggtgcagcaa agattttttg
     2641 gaagtcccga tacaaggcca ggctacaccg gactcagatc ctgccatagc agaaaaattg
     2701 gttacgggag tttataattc catgcttgca ggaagtgcat tcggtggaat tggagatgtc
     2761 cacggaatca gtttcattac tgccacaatt attatgactg atgatgctga aaagggaagt
     2821 actgcctccg atcagcctaa tatcgcggcc atagacaatt ttgaacatac ctccaccaat
     2881 aattttgtgg cggcactatg gacgggaaat tataatgcga ttgccaaggc taatcaagcg
     2941 ctaaaggctc tggatgttgc tcccatcgct gatgcaatta aaacgcaata tcagggagaa
     3001 gtccggtttc tgagagctta tttttacttc aatctggtac ggtatttcgg aggtgtcccg
     3061 aaagttttgc agattccggc agatgccaat gaggcaaatt cgaatcctgt gtatcaaacc
     3121 cgtgcaagtg ttgatgagat atacagcctg attcgtgaag acctgaacta cgccattgaa
     3181 catctgaaac caaggtctca gtctcaggtt ggacgggcca ataagggcgc agctcaagcc
     3241 atgttggcca aagtcgaact gactaccagg aattggcaaa atgcctataa tttatgtcag
     3301 gaagtaatca attccggaca atacagcttg ttggcaaagt atgaagatat ctggcgacag
     3361 gttggcgaca atagtgtgga atctattttt gaagtgcaga caggccagtt taataattct
     3421 gactttggag tagaggggta ttgtgtttgg caggggccaa gagtcggagg taagggtggt
     3481 tggagagatc ttggctttgg attcaataat cctacggtag atctggtcaa tgaatatgaa
     3541 cccggtgata tacgaaagga tgcaaccatc atatttattg ataatagcgg aactcataag
     3601 ggaacaatac tttttgatgg ctatcgcatc ccaagtcagg atagcgtcca aaatgcacgg
     3661 tacaattata aagcctatca ttcagagaat aaggagatag aatccttctt cggcaaccgt
     3721 gatagaaaag ccaagaatat ccacttgctg aggtatggag acctattgct tatggccgct
     3781 gaatctgcta atgaattggg caatacggga gaagcagcga cttatctcaa tatgatccgg
     3841 gaaagagcaa ccctttccgc gactacagcc tcttctcaag ccgatatgcg cgaagcaatc
     3901 tatcatgaaa gacgtgtaga gatggcgatg gagcatgacc gttggtttga tctggtcagg
     3961 actgggcgtg cagccaaggt tatgaagccg ttgaagcctt cctttactga aggcaagaat
     4021 gaattgttgc cgattccggc actacagatt gccttgagtg gaggaaaatt attgcaaaat
     4081 cccgggtatt gatgcctgtg tctttagaat ttatttaatt atattataaa tttatcaaaa
     4141 ttttaaaaca atggttatta aaaattttaa gcaaattgcc ttgtttgtat tggcaattgt
     4201 agcaatcagt ttcacttctt gtaaagacga tgacaacaaa tta
//
LOCUS       DAOQQN010000294         4232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9839_length_4232_cov_5.548240, whole genome shotgun sequence.
ACCESSION   DAOQQN010000294 DAOQQN010000000
VERSION     DAOQQN010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9839_length_4232_cov_5.548240"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PLG24_10435"
     CDS             <1..582
                     /locus_tag="PLG24_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HMZ25351.1"
                     /translation="SATDSYDIAQSAANTDICSEMFDGDAQDPAAQSKLDFDRCFAFR
                     NFTLVKDPMVYEFSSIDTDPRTRMIERENDYFTLFDFSAKYDPVPTMLTQNHTRTVKG
                     FMGQTTAFMRKYVKPNVLVMGENKALGEAKYIHGEYGKGQWTFYGGHDPEDYEHRVGD
                     PPTDLHLHPNSPGYRLILNNVLFPAAKKQKQKT"
     gene            complement(831..2372)
                     /locus_tag="PLG24_10440"
     CDS             complement(831..2372)
                     /locus_tag="PLG24_10440"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HMZ25352.1"
                     /translation="MDKKLFILREKQSRALSGGGSHRIDSQHAKGKLTARERIQLLLD
                     EDSFEEIGQLVEHRSHDFGLEREIYPGDGVVTGYGRIDGRLVYVYSQDFTVFGGSLSE
                     THAEKICKIMDLAVKNGAPIIGLNDSGGARIQEGVNSLGGYADIFYRNTLASGVVPQL
                     SAIMGPCAGGAVYSPAITDFIIMVEQTSYMFVTGPNVVKTVTNEDVTSEELGGASAHA
                     TKSGVTHLTAVDDVDCINQLKKLLSYIPQNSVDLPFDLPYTPGDEWRPKLNDLVPVDS
                     NKPYDMKEIIGELVDIGSFFEIHGEFAENMVVGFARLAGKSIGIVANQPMSLAGVLDV
                     DCSKKAARFVRFCDCFNIPLLVLVDVPGFLPGTDQEWNAIITNGAKLLYAFCDALVPR
                     ITVITRKAYGGAYDVMNSKHIGADLNYAWPGAEIAVMGAKGASEIIFKREISEADNPA
                     AKAKEKETEYADNFSNPYRAAARGFIDEVIEPSITRKKLISGFAMLENKTGQRPRRKH
                     GNIPL"
     gene            complement(2389..>4232)
                     /locus_tag="PLG24_10445"
     CDS             complement(2389..>4232)
                     /locus_tag="PLG24_10445"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HMZ25353.1"
                     /translation="ERMPNSDPYNVRHVLNSLFGASLIIFTGLLARHLSRSWFVALIA
                     LLLTVLSPRLFGDSMNNPKDIPFAMAYVMSVLGILRFNSLLPKWDWRSAIFLGVSMSI
                     ALNIRVGGLLLLVYFGLYTIVNVYLKRKEFDSAKVNGLATIGKAAILGICSYFVGLIF
                     FPYSHAAPITNTLEALKIMSNFDYAIRMLFEGHALWSDEIPWYYIPKWLGMAIPLSVI
                     IGLIMFLVRIRSIFKRLDWLPLAFILFVGIFPVVYAVAKHSSLYDGIRHFLFLMPMIN
                     VMAALGWANVMFAFWAKKFKWAVPAVLGLLLVLPLRFMIANHPNEYIYFNELSGGIKK
                     AYGNYETDYWMNSMKELSKWLISHDERIRKGEAVIVCTNSIDPVKHYFERWAPNVKVI
                     YAAFKDRYKHKADYYLSIPRFIDSDLLKNGSWPPKEVVHSVTVDGVPVGALSKYVDTF
                     TYAGLNATKMQDYLLANEYFKKAYANDDKNNVAIANLALNYVNQDSLAAASQWADRGL
                     ALSPDNEDFLLPKGMILMYQGKVAEAYEYFDRVKKMNKRNVLAYYYSAIIMENQKNYS
                     AALDNLQKVLEQAPQFKQAYLLGAQIMQNSGNPEAAARYMEAANNLK"
BASE COUNT         1257 a         1017 c          818 g         1140 t
ORIGIN      
        1 tcagctactg attcatatga catcgcacag tctgcagcca atacggatat atgcagcgaa
       61 atgtttgatg gagacgcaca ggatccggca gcacaatcaa aactggattt tgatcgatgt
      121 tttgccttca ggaacttcac cctggtcaag gatcctatgg tatacgaatt cagcagtatc
      181 gatacggatc cgcgcaccag gatgatcgaa agggagaatg attatttcac cctgtttgac
      241 ttttcggcga aatacgatcc ggtaccaacc atgctcacac agaatcatac gaggacggtc
      301 aaaggtttca tgggacagac aacagccttt atgcgtaaat atgtaaagcc aaatgtactt
      361 gtcatgggtg aaaataaagc actcggtgaa gcaaaataca tccacggaga atatggaaaa
      421 ggacaatgga cattttatgg tggacacgac ccggaagatt atgaacaccg tgttggcgat
      481 ccaccgaccg acctgcacct ccatcctaat agtcccggct acaggctgat tctcaacaac
      541 gtactttttc cggcagctaa gaagcaaaag cagaaaacgt gattaatcta taattaggcc
      601 acttgtgaat tctaatgaca aataaaattg tcaaagaagc aatatcgaag gtacattcat
      661 aatagaccaa ttccattgac ctaaacgatt gtaaccccgg caatatacct gataattttg
      721 gactattata tagatcacct tggtataata taaaagcata gaacacgatt tcgtcctatc
      781 cggctggaat tcgaatcaga caatgtccta aatgaatatc ccagtcaacc ttacaatggg
      841 atattcccgt gctttctcct cggtctctgt ccggttttgt tttccagcat agcaaatccg
      901 gaaatcagct tcttacgtgt gatcgatggt tcgatgacct catcgatgaa ccctctggcc
      961 gctgcacgat aaggattgga aaaattatcc gcatattcag tttccttctc tttggcctta
     1021 gcagccggat tgtcggcctc tgatatttca cgcttaaata taatttcact ggcccctttg
     1081 gcacccatca cggcgatctc tgctccaggc caggcataat taagatctgc tccgatatgt
     1141 ttggaattca tcacgtcata tgccccacca tatgcctttc gcgtgatgac tgtgattctt
     1201 ggcaccaaag catcacagaa tgcatacaat agtttggcac cattggttat gatggcattc
     1261 cactcctgat cggttcccgg caaaaagccc ggtacatcga ccaacactaa aagcggtatg
     1321 ttgaaacaat cacaaaaccg aacaaaacga gctgctttct ttgaacaatc tacgtccaac
     1381 acacccgcta agctcatcgg ctgattggcc acgatgccaa tagatttacc tgcaagccgt
     1441 gcaaaaccta ctaccatatt ttctgcaaat tcgccatgga tttcaaagaa actaccgatg
     1501 tccaccagct cccctatgat ttctttcata tcatagggct tgttgctgtc cacaggcacc
     1561 agatcattaa gcttcggcct ccattcatct ccgggggtat agggtaaatc aaatgggaga
     1621 tctacgctgt tttgcgggat ataactcaat aattttttca actgattgat acaatccaca
     1681 tcatccactg ccgtcaaatg agtaacaccg gacttcgtag catgagcaga agctcctccc
     1741 aattcctcgg atgtcacatc ttcattggtg acagtcttta caacattagg tccagtgaca
     1801 aacatatagg aagtctgttc aaccatgatg atgaagtccg taatggcagg gctatatacg
     1861 gctccgcctg cgcaaggccc cataatggcg ctaagttggg gtactacccc actcgcaagt
     1921 gtattacgat aaaagatatc ggcgtatcca cccaatgaat tgaccccttc ctgaatcctg
     1981 gctcctccgg aatcattgag tcctatgatc ggagcaccgt tcttcacagc caggtccata
     2041 atcttgcata ttttctcggc atgcgtctct gaaagggaac ctccaaacac cgtaaaatcc
     2101 tgagaatata catacaccaa tcgtccgtca attcttccat aacctgtcac aacgccatca
     2161 cccggataga tttctctttc caacccaaaa tcatgtgagc ggtgttcaac caactgtcct
     2221 atttcctcaa aagaatcttc atcgagcaac agttgtattc tctcacgtgc tgtaagcttt
     2281 cccttggcat gctgactgtc aatccgatgt gaaccaccgc cactcaatgc cctggattgc
     2341 ttctctctca gtatgaaaag ttttttatcc atttcctttg attagaattt acttcaaatt
     2401 gtttgctgcc tccatatacc ttgcagctgc ttccggattt ccgctatttt gcatgatctg
     2461 tgctcccaat aaataagctt gcttaaattg gggtgcctgt tccagaactt tttgcagatt
     2521 gtccaaagca gcggagtaat ttttctgatt ttccataatt atagcgctgt agtaataggc
     2581 aagaacattg cgtttgttca ttttcttgac ccgatcaaaa tattcgtaag cttctgctac
     2641 ttttccctgg tacatcagaa tcataccttt aggcagtaga aaatcttcat tatcaggcga
     2701 taatgcgaga cccctatcag cccattggga ggccgcagcc agtgaatcct gattgacata
     2761 attcaaggct aaattagcaa tggcaacatt attcttgtcg tcattggcat aagccttctt
     2821 gaaatactca tttgccaata agtaatcttg cattttggtc gcatttaagc ctgcataagt
     2881 aaatgtatca acatatttgg acaatgctcc aaccgggaca ccatctacgg taacagaatg
     2941 caccacctct ttcgggggcc aactcccgtt ctttaataaa tcactatcga taaaccgcgg
     3001 gattgataga taatagtcag ctttatgttt gtaacgatcc ttaaaagcag catagattac
     3061 cttgacattc ggcgcccagc gctcaaaata atgcttgacg ggatctatac tattggtgca
     3121 tacaatgacc gcctcgcctt tcctgatcct ttcatcatgt gagatcaacc acttagagag
     3181 ttctttcata ctgttcatcc aataatcggt ttcgtagttg ccataggctt tcttgatacc
     3241 accggataat tcattgaaat aaatatattc attggggtga ttggcaatca taaatctaag
     3301 tggaagtact aacaggaggc ccagaacagc cggcaccgcc catttaaatt ttttagccca
     3361 aaaggcaaac atcacattgg cccaacccaa tgctgccatc acattgatca ttggcatcag
     3421 aaagagaaaa tgcctgattc cgtcgtacaa ggaactgtgt tttgcaactg catagaccac
     3481 aggaaatatt ccgacgaaaa ggatgaaagc cagcggcaac cagtccagtc tcttaaagat
     3541 ggaacgtatc ctgaccaaaa acattatcaa cccgataatg acagaaagcg gaattgccat
     3601 acctagccat ttagggatat aataccaggg aatctcatcg gaccataaag catggccttc
     3661 aaatagcatc ctaatggcat aatcaaaatt ggacatgatc ttcagagcct ccaatgtatt
     3721 ggtgataggt gcagcatggg aataaggaaa gaatatcaat ccaacgaaat aggaacagat
     3781 acctaagatg gctgccttac ctatagttgc caggccattt actttggcgc tgtcaaattc
     3841 tttacgtttc agatatacat ttacaattgt ataaagacca aaatacacta gtaacagtaa
     3901 gcctcctacc ctaatattga gggctatcga catagaaact cccaaaaata ttgccgaacg
     3961 ccaatcccat ttaggcaata aagagttgaa ccttaaaata cctaataccg acatgacgta
     4021 cgccatcgca aatgggatat ccttagggtt gttcatgctg tcgccaaata gtcgtggtga
     4081 gagcacggtc aataataagg ctatcaaagc tacaaaccag cttcgggaca aatgccgtgc
     4141 caaaagaccg gtaaagataa ttagtgatgc accgaataag gagttgagga catgacgcac
     4201 attataagga tcagaattcg gcatacgctc gt
//
LOCUS       DAOQQN010000295         4227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9860_length_4227_cov_5.256472, whole genome shotgun sequence.
ACCESSION   DAOQQN010000295 DAOQQN010000000
VERSION     DAOQQN010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9860_length_4227_cov_5.256472"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="PLG24_10450"
     CDS             complement(<1..465)
                     /locus_tag="PLG24_10450"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HMZ25354.1"
                     /translation="MPDYSDNIETKTHGIQHVMPVLQTLNCRGSILDISKPLVMGIIN
                     ITDDSFYSGSRTPGPEGVVKKAKEMIHAGASIIDLGAMSTRPGSDEIPAGLEIERIIP
                     AVEAILSVVPEAILSIDTYRSKVAEKAIQAGAAIINDISSGDIDPDLPFLALQ"
     gene            1001..3160
                     /locus_tag="PLG24_10455"
     CDS             1001..3160
                     /locus_tag="PLG24_10455"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HMZ25355.1"
                     /translation="MKIKSFLIFALIIGVVSLFALYPRPIGDPIEKEVVILKSVMNIL
                     DKAHFKPKDINDEFSEKVYKSYLESMDGGKRFFIQSDINQLKPYATLIDDEIKGSKLD
                     FFEKSLNLITMAQKRVREYYKEILSKPFSFDKKETISYDYEKLPYAKSEKELKARWEE
                     TLRAAVLDEVIQVMNRQENDSLKVNPEEKKSFAVIEKEAREKVGKDYDRLFDRLEKLR
                     REDRFGDFMNSITEQFDPHTSYFSPSDKEDFDIGMSGKLEGIGARLQADGEFTKVSEV
                     VVGGPAWKEKNLEAGDLIMKVAQDGQPAVDIRGMRLDDVVKKIRGKKGTKVILTVKKV
                     NGSVQDITIVRDEVLMDESFAKSVIVGKKGVMENVGYIYLPKFYADFDNNKGRFCYTD
                     VAKEIEKLKQQNVNGIILDLRNNPGGSLNDVVKMAGLFIENGPIVQVKSRDQEPYVMN
                     DDNKSYAYDGPLVILVNHQSASASEIMAAAMQDYKRAVVIGGTSTYGKGTVQRFIDLD
                     RTLSSSSNLRPLGQLKLTIQKYYRIDGGSTQLKGVTPDIILPDALNYIKVGEKENKTA
                     MDWSKINSAQYSQDVFVVRNMDEIKSRSHNRTENNPLFVAIDENAKRIKAQNDQKEFS
                     LELNTYRSELQKRKDDNKKFNGLFKEISGLEVANLPVDMKLIERDSASMARNDNFLKN
                     LRKDVYLDEAINVISDLQSNRITLMDKNSGRVKTGKE"
     gene            3280..3789
                     /gene="lptE"
                     /locus_tag="PLG24_10460"
     CDS             3280..3789
                     /gene="lptE"
                     /locus_tag="PLG24_10460"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HMZ25356.1"
                     /translation="MERSGSSKYLSMILVISLLVSGCYSFRGTSIPSNTNTFYVGNFT
                     LDTYDAPFNLSQMVTEKLKDKIRKETRLKYVEIEPDIEFVGKVTNYVVSPVAPQANQI
                     PSANRLEITINVSFLDQKNEKNKWTSNFTFFKDFSKDANLIDVRDGLANDIFDQLVED
                     VYNRAFTNW"
     gene            4044..>4227
                     /locus_tag="PLG24_10465"
     CDS             4044..>4227
                     /locus_tag="PLG24_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25357.1"
                     /translation="MELVERLLHFPSMLRSIEESVLVKNLLLYPYFGVLYLLQALKRQ
                     FKALDQEEEYFPPVLAN"
BASE COUNT         1310 a          682 c          954 g         1281 t
ORIGIN      
        1 ttgtaaggcg agaaatggca agtcgggatc gatatcaccg gatgaaatat cattgatgat
       61 tgccgctccg gcctgaatag ccttttccgc aactttcgat cggtaggtat caattgaaag
      121 gatggcttca ggtactacgg aaagaattgc ttcaactgca gggattatcc tttctatttc
      181 cagcccggca ggaatttcgt ctgagccggg tctggtggac attgcaccaa gatcaatgat
      241 ggatgcccct gcatgtatca tttctttagc ttttttgaca actccctccg ggccgggtgt
      301 cctgctcccg gaatagaaag aatcatcagt aatattgatg atacccatga ccaaaggttt
      361 actgatgtcc aggattgaac cccggcaatt caaggtctgc agaaccggca taacatgttg
      421 aatcccatgt gtttttgttt ctatgttgtc agaataatcc ggcataatca tttgggtagg
      481 tgagcgatgg agtatggtgt ttcaagacaa aattcacgag atacatcaga ttcctggtta
      541 agaaaatgaa tttgacttta ttaaatccgg aaccatactg gtttgaattt gtgctgtata
      601 tagcaagttt aatggccatg tccatggatt aatatacaaa ggtaggaaaa cgcaatgagg
      661 ttttttaacg agttttctta tatgtgttcc cgctaacaga cttggtctgt ctgttgaggc
      721 ctggctttga ctgtttgatg atttctattt tttaataatt gaatgaaaaa aacatattat
      781 ttcctgttta atctatattt tcttgatctt aaaagtccat attgttatac acactattgt
      841 actttagagg aatgccatcc aatgaaattt tctatttttg tctaaaaatt agttaatcct
      901 gtcttaaaaa ttgcaattat ccaactatta agggataata atgttaagat tttcgtttac
      961 tattgtgctt agaaaattat aacgattgaa agaatttctt atgaaaataa agtctttttt
     1021 gatttttgcc ctcattatag gggttgtaag cttatttgcc ttatatcccc gaccgattgg
     1081 cgatccgata gaaaaggaag ttgttatcct gaaatcggta atgaacatcc tagacaaagc
     1141 ccatttcaaa ccaaaagata tcaacgatga attctcagag aaggtatata aaagctatct
     1201 tgagagtatg gatggtggaa agagattttt tattcaaagt gatatcaatc aattgaagcc
     1261 ctatgctacc ttaatcgatg atgagattaa aggaagtaag cttgacttct tcgagaagag
     1321 cctgaatttg ataactatgg ctcagaaaag ggtcagggaa tattacaagg aaatattgtc
     1381 taagccattt tcttttgaca aaaaggaaac catctcttat gattacgaaa aacttcctta
     1441 tgctaagagt gaaaaggaat tgaaggccag gtgggaagag acacttaggg cagcagttct
     1501 ggatgaggtc attcaggtta tgaacaggca ggaaaatgac agcttgaaag tcaatccaga
     1561 ggaaaaaaag tccttcgcag tgatagaaaa agaagccagg gagaaagtag gcaaggatta
     1621 tgatcggttg tttgatcggt tggaaaagtt aagacgggag gatcgttttg gcgattttat
     1681 gaatagcatt acggaacaat ttgatcctca taccagctat ttcagtccga gtgacaagga
     1741 ggattttgat atcggaatgt ccggcaagtt ggaaggtatt ggtgcccgtc ttcaagcgga
     1801 tggtgaattt acaaaagtct ctgaagttgt tgtcggggga cctgcatgga aagagaagaa
     1861 tcttgaagca ggggatttga ttatgaaggt tgcacaagac gggcagcctg cagtggatat
     1921 tagaggcatg cgtttggacg atgttgtaaa aaagatcagg ggtaaaaagg gcaccaaggt
     1981 tatccttaca gtcaaaaagg tcaatggcag tgttcaggat ataaccattg tcagggatga
     2041 ggtattgatg gatgaatctt ttgcaaagtc ggtgatcgtc ggcaagaagg gagtgatgga
     2101 gaatgtgggt tatatctatc tgcccaaatt ttatgctgat tttgataata ataaaggacg
     2161 gttctgctac accgatgtgg ctaaggaaat agagaagctg aagcagcaaa atgtcaatgg
     2221 tatcatcctg gatctaagga ataatccggg aggttctctg aatgatgttg ttaagatggc
     2281 cgggttattt atagaaaacg ggcccatcgt tcaggtcaaa tctagagatc aggagcccta
     2341 tgtcatgaat gatgataata aatcctatgc gtatgatgga cctttggtga tactcgtcaa
     2401 ccatcaaagt gcatcggctt ctgagattat ggctgctgcg atgcaggatt ataagcgcgc
     2461 agtggttatt ggaggaacat ctacttatgg aaagggaaca gtgcaaaggt ttattgacct
     2521 ggacagaacc ctttcaagca gcagtaacct taggccattg ggtcaattga aactcaccat
     2581 tcaaaaatat tacagaatcg atggtggtag tactcaactc aaaggtgtta cacccgatat
     2641 tattttgccg gatgcgttga attacattaa agtgggagag aaagagaata agacagctat
     2701 ggattggtcg aagattaatt cggcccaata tagccaggat gtatttgttg tcaggaatat
     2761 ggatgagata aaatccaggt ctcacaacag aactgaaaat aatccattgt ttgttgcaat
     2821 tgatgaaaat gcaaaaagaa ttaaggcgca gaatgatcaa aaggaatttt ccctggaatt
     2881 gaatacatac aggagtgaat tacagaaaag aaaagacgat aacaaaaagt tcaatggcct
     2941 gttcaaggaa atttccggtc ttgaagtagc taatctaccg gtagatatga aacttataga
     3001 gagagatagt gcaagcatgg ccaggaatga taattttctt aaaaacctga gaaaggatgt
     3061 atacctggat gaggctatca atgttatttc tgatttgcaa tccaacagga taaccttgat
     3121 ggataaaaat tccggaaggg taaaaacagg taaggagtaa gagctatttt tgccggcaca
     3181 cttgaggtta taatgtgcct atatgaaaac gaaatttatt gtatgattcc ataggataga
     3241 tttcgttttc gttttttata cttgcataaa attgtattca tggaacgatc aggaagcagc
     3301 aaatacctct caatgatact ggtaatttcg ttattggtct caggttgcta ctcatttcga
     3361 ggaacctcaa ttccatccaa tacgaatacc ttttatgttg gtaatttcac gctggatacc
     3421 tatgatgccc ctttcaatct atctcagatg gtaaccgaaa agctgaaaga taaaatcaga
     3481 aaggagacca gattgaaata tgttgaaata gagccggata tcgagtttgt tgggaaggtt
     3541 accaactatg tcgtgagtcc tgttgcccct caggccaatc agattccatc ggccaatagg
     3601 cttgaaatca caattaatgt cagctttctg gatcaaaaga atgaaaagaa taaatggact
     3661 tccaacttta ccttttttaa ggattttagt aaagatgcca atttgattga tgttcgggac
     3721 ggtcttgcca atgatatctt cgatcagttg gttgaggatg tatataatcg ggcatttacc
     3781 aattggtagg gattgattat tgagtgggcg cttaagctgt tttagtctcc tttttatatt
     3841 cagattgtca ttcatacttt ggagcaatac gaggttggca ctatgctctc cggaaatgat
     3901 cttattaatt tttctatgaa agacatcctg tctacatggt gattaacatt gaatatgctt
     3961 gatcctaccg ggcctttcct tctgattttt atttgtgcaa atttgttatc ttgcagcgtt
     4021 tttgactgaa tgctaaattg tatatggaat tggtagagcg cttgttacat tttccttcga
     4081 tgcttagatc gattgaagaa agcgtattgg tcaaaaattt gcttttatac ccttattttg
     4141 gtgttctgta tttacttcaa gccctaaaaa ggcaatttaa agctttagat caggaagagg
     4201 aatatttccc tcctgtttta gccaatg
//
LOCUS       DAOQQN010000296         4206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_9941_length_4206_cov_5.322573, whole genome shotgun sequence.
ACCESSION   DAOQQN010000296 DAOQQN010000000
VERSION     DAOQQN010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9941_length_4206_cov_5.322573"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            781..1215
                     /locus_tag="PLG24_10470"
     CDS             781..1215
                     /locus_tag="PLG24_10470"
                     /inference="COORDINATES: protein motif:HMM:NF025032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HMZ25358.1"
                     /translation="MNHKLTLLLILGLVFGLMSSCKKEDKSSRKELLTATSCWKISKY
                     EEKDESGKYVDVTEENYETCELDDCNRFAADGNFTVTDDGEKCSGSSTQSGTWTLSAD
                     EKQLTINTVFFPIILNIESMDSNTLVVTYTLFETEARVTYKN"
     gene            1662..2444
                     /locus_tag="PLG24_10475"
     CDS             1662..2444
                     /locus_tag="PLG24_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HMZ25359.1"
                     /translation="MIDTHAHLYAAEFDADRPEMLQRAKDVGVRQIYLPNIDLASIDA
                     MHRLEDSSNGFCISMMGLHPCSVREDFRDHLAQLKLWLDKRSYAAIGEIGIDLYWDKS
                     TFDIQVEAFVEQIKWGNEYNLPICIHSRESTREVLDVLYANRNVLTGGVLHCFSGSLE
                     EAEEALDLGFYLGFGGSATYKNTNLVPVIMAVPDECILLETDAPYLTPVPYRGKRNES
                     SYIPIIANRIAEIRGTSVEEISSITTENALRLFGNKSVPGQP"
     gene            2441..3469
                     /locus_tag="PLG24_10480"
     CDS             2441..3469
                     /locus_tag="PLG24_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HMZ25360.1"
                     /translation="MNLFATDTWRFLSLVRPAKVLNAAKVVASYYLTRWTGKAIQWGL
                     PFTISIEPTTACNLRCPECPSGLRAFSRPTGNLKEDFFRKIIDELGNKLFYLIFYFQG
                     EPYINPKFLEMVHYASQKNIYTITSTNGHFLDDENARKTIESGLSRIIISVDGTTQEV
                     YQSYRREGNLETVLNGARNLVRWKNELNSPTPHIIFQFLVVKPNEHQIPEIYRLAKEI
                     GVDEVKLKTAQIYDYEQGNELIPSIDKYSRYRKQANGTYRIKNQLLNHCWKLWHSCVI
                     TWNGTVVPCCFDKDAKYMLGDLKKDSFRKIWYGKLYDGFRRKILAGRDQIDICRNCTE
                     GCKVWESE"
BASE COUNT         1296 a          734 c          825 g         1351 t
ORIGIN      
        1 gttaccgata taacaggcat cgcatgaaag gagagatttt ggatgatatt attacgagaa
       61 tggtcaggca taagccgatg acgcagagaa tgatgatagc gcactgatta tagtacaatg
      121 agtcctgtcc tttctgtttg acttttgcaa aatattttat tccaaaaaca aaagagttgt
      181 gaataaggtg gaagtgtcag aatgacttgt tggttataaa ttcaaagata tttttcaaaa
      241 atttggatca taaaagtttt ttacttacat cagtatagtt atctacagaa aatttttgca
      301 accgattttg tcagtgaagc cctttaattc attttaactt cctgattgtg cgaaaggatt
      361 atctattttc aataccttct tggttgtaag aggccggatt aaacttccac tttccaaggt
      421 gtttggatgt aaatgttgga caatgccatt gagcctctaa ttttttgtac tttttattag
      481 atattttatt tggtattgat ttttttgtat gtttgaatac cgagttttca agtcttggat
      541 tttatactgt tttttcagtc gggatacatt ttaattaggt aatttactga tttttattac
      601 tctatgtttt ctgtaaaata aaataattaa ttgggatatt gatttgaaat actcattttg
      661 ttatattttt tttgataata taaatattat taattatttt tgcaaaacat ttaacaccgg
      721 aaaatttaaa gctattcacc cgttttttgt aaatttttta accaaatatt attttaagtt
      781 atgaatcaca agttaacact tttattgatt ttggggttag tcttcggact tatgtcatct
      841 tgtaagaaag aagataagag ctcaaggaaa gaattactta ctgcaacctc ttgctggaaa
      901 atttcaaagt atgaggaaaa ggacgagtca ggcaaatatg ttgatgtgac tgaggaaaat
      961 tatgaaacct gtgaacttga cgactgcaat agatttgctg cagatggaaa ttttacggtt
     1021 actgatgatg gagagaagtg tagtggatct tctacgcaat ccggaacatg gactttgagt
     1081 gctgatgaaa agcaactcac gattaatact gttttttttc caattatatt gaatatagaa
     1141 tcaatggatt ctaatacact tgtcgttact tatactttgt ttgaaactga agcaagagta
     1201 acttataaga attaatatcc atattctttg aggtcttagg gctcctgctt tgaatgtagg
     1261 agcttttttt atttatcacc tgaaattata catatttata ttttattatg tttaatcaat
     1321 acgataaaag gatttatgtc ggtatcaaaa tggagaaaca tcagtgaaaa atggatatct
     1381 gttgtaaaat tagtagtagg tagatgatta ataaccacaa atattgatgt tagtgagaca
     1441 tgctgaaaaa ccagctataa tttttacaaa ataacttata tttttttaag agattatttt
     1501 caaatacact ggtaaatgac atagagccta ttaatcatct acatacttcg ttttttcgat
     1561 cccacttcca caccctctcc cccctattcg gtttttaatt gcatattctt tcattcaacc
     1621 ttatctgctt acctttgttc tttggaaaat tgatttatac catgatcgat acccatgccc
     1681 acttgtatgc agccgaattt gatgccgata ggcctgaaat gctgcaacgc gccaaagacg
     1741 taggtgttcg acaaatttat cttcccaaca tcgacctcgc tagcattgat gcaatgcata
     1801 gactggaaga ttcctccaat ggcttttgta tttccatgat gggacttcat ccttgttcag
     1861 taagagagga tttccgagat catcttgctc aattgaaact atggcttgat aaaaggtctt
     1921 acgctgcgat tggcgagatt ggcatcgatt tatactggga taaatcaacc ttcgacatcc
     1981 aagtcgaagc ctttgtggaa caaatcaaat ggggcaatga atataatctg cccatttgta
     2041 tacattccag agaaagtaca agggaagtcc ttgatgtgct ctacgcgaat aggaatgtcc
     2101 tgacaggtgg tgtattacac tgcttttcag gttctttaga ggaggctgaa gaagccttgg
     2161 accttggttt ttatttgggt tttggaggct ctgcaaccta taaaaatacc aatctggttc
     2221 ctgtgattat ggctgttcct gatgagtgta tcctcctgga aaccgatgca ccctacctta
     2281 caccggtacc gtaccggggc aaaagaaatg aaagctccta tatcccaatc atagccaatc
     2341 gtattgcaga gatccgggga acttcagttg aagagatttc ttccatcacg accgaaaatg
     2401 ccctccggct ttttgggaat aagtctgttc cgggccaacc atgaatttat ttgctacaga
     2461 tacatggcgt tttttatcct tggtacgacc tgccaaggtg ctaaatgctg ccaaagttgt
     2521 tgcctcctac tacttgaccc gatggaccgg gaaggccatt cagtggggct taccctttac
     2581 cattagtatt gaaccaacga ccgcttgcaa cctccgctgt ccagaatgcc caagtggcct
     2641 gagggctttc agtcgaccta caggaaattt gaaagaggat ttttttcgta agatcatcga
     2701 tgaactcggt aacaaattgt tttatctgat tttttacttt caaggagaac catatatcaa
     2761 cccaaagttt ctcgagatgg tccactatgc ctctcagaaa aatatttaca ccatcacctc
     2821 aaccaatggc catttcctgg atgatgagaa tgctcgaaaa accatcgaat caggactcag
     2881 ccgcatcatc atttcagttg atggaacaac acaggaagtg tatcaaagtt atcgacggga
     2941 aggcaacctg gaaacggtac tgaatggagc cagaaacctc gttagatgga aaaacgaact
     3001 gaattcaccc acccctcaca tcatatttca gtttttggtc gtcaaaccca atgaacacca
     3061 gatccccgaa atataccggc tggcaaaaga aattggcgtg gatgaagtca aattgaaaac
     3121 cgcccagata tatgattatg aacaaggaaa cgaattgatt ccatcgatcg acaagtacag
     3181 tcgctatagg aaacaagcta atggcaccta tagaattaaa aaccaattgc tcaatcattg
     3241 ctggaagctg tggcatagtt gcgtaattac atggaatgga actgtggtac cctgttgttt
     3301 cgataaggat gccaaataca tgcttggcga cctaaagaag gatagcttca ggaagatctg
     3361 gtatggtaag ctatatgacg gatttagaag aaaaattctg gccgggcgtg accagataga
     3421 tatttgccgt aattgtacag aaggttgcaa ggtttgggaa agcgaataaa ccacataggt
     3481 tgcacggcca aagtatctgt gctttataat tacagaatgc aacaagaaat agtatagact
     3541 aaaaactgtt tttgcaagga accggacacg tatcgttatt acaatatttt aggaaaggga
     3601 gccaaattcg gttagatcat ttattttgtt gttgcaatag aggatgatgt gatgagaggc
     3661 tcataaatag tcaagaacta aattttgtca gtagcaggtg ctatcccaca tttaatcgct
     3721 gtttattcaa agtagtagga caccattcct tcaaaccacc aaaaggaagc taaaaataat
     3781 gagcagtaag tattttgagc tatctattgt aggagcagta catcgagttt tagaaagact
     3841 acattttttc cttattcata ttgaggaaag atgcaaaatg tataaaaagc agattatcaa
     3901 taatctttcg ccaattcaat ttgtaatctt tattcggata ctctatcttt cgtcattgct
     3961 gatgatatct gctatggcta ttaacgcaca tggtaattta tcacttaaca ttttacaaaa
     4021 ttgactattt tcacggtttg tgtagatatt aactaatgct gatttttatg aatggaggag
     4081 aaatgattgc ccgcattttg acaaagtatg gtgtacggtt tctttttact ttgtgtggcg
     4141 gtcacatttc gccaatcttt gtgagtgctg aaagagcggg aatccgtgtc attgatacca
     4201 gacatg
//
LOCUS       DAOQQN010000297         4175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_10060_length_4175_cov_5.074757, whole genome shotgun sequence.
ACCESSION   DAOQQN010000297 DAOQQN010000000
VERSION     DAOQQN010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10060_length_4175_cov_5.074757"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1878)
                     /locus_tag="PLG24_10485"
     CDS             complement(<1..1878)
                     /locus_tag="PLG24_10485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HMZ25361.1"
                     /translation="MRFFQTFFLFLLSLTAFSSNYNGEIKTFSQPDGSQVDVRLFGSE
                     FYMRAEGLDGYTLVRDDASKWICYARLSEDGRELIATEYRYFGSQSGGATLNVDPGVP
                     KHLDIRPEYVQEVMNHNAQSLFGLPTVEAVQNSYTTYQRSGEVLQGNLKGLCIIVDFS
                     DEVATLKSEDYIDFCNGLNYVRFGNNGSLRQYYLDISGGLLDYQNVVFGIFRAPRTFQ
                     EYDAMPYAVGAQQILDLALNWIKSTGFDFSTLSTNPDKTIQAINLMYTGNPPSWAKGM
                     WFHQGYYSGFSANGVRSGRYNTSPAKSPLTIGTVVHENGHMICKWPDTYKYDNKSGPD
                     GIGAFDVMCSGGPDANPTLPNPYFMHRIGWLRDTDVTGKNEILTDTANAYAAYSYRNP
                     DIPTEYYMFQNRLKTGRSEGIPDEGLVVWRINENGDNQTTNHQVYVVHQNNNINDHSG
                     ACFRKGRMAEYNDSTTPSSRWWISDIPSGLRLWDFSNKGPVMTYKIGRGPSLTANYLN
                     YDNDSNADGYITGGEEFDLNVRLFNVDAGLSSSSIVTCTPIGTNATKVNVLTASIQPG
                     EIASGDSLDITFRIVAEAALKDFETVEFKIDIEEDGRSFYTYASIVTGTVYIMDSTTI
                     ID"
     gene            complement(2142..3248)
                     /locus_tag="PLG24_10490"
     CDS             complement(2142..3248)
                     /locus_tag="PLG24_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HMZ25362.1"
                     /translation="MRIAVNTRFLLKNKLTGLGHFTLEVVRQLVLNHPDDTFIFLFDR
                     PYDSSFIFAPNIKPIIVHPPARATLLFTIWFEWSIPAILRSSKADVFFSPDNFLSLRT
                     RLPTLLVIHDLAYLHTPDGFKGMQLLYYRYFMPKFARRADRIATVSEATRQDILQQYD
                     IAPDKVEVVGLGLNEGFEFEGITVRQDYFVHLGTIQPRKNVIGLLRAFELYKNRTGRL
                     TKLKFIGGKGWNDEEFYTILDKHSYKQDVELLGYRSNEEISHILNHASALLCVSYFEG
                     FGMPVIEAQRCGCPVIASNTSSLPEASGGAALLVNPSDDNAIADAMKKIMEDSLLRAE
                     LIEKGFNNIKRFSWEKTADAIYSLLQELYKQGVP"
     gene            3437..>4175
                     /locus_tag="PLG24_10495"
     CDS             3437..>4175
                     /locus_tag="PLG24_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HMZ25363.1"
                     /translation="MLKIFDFRTYWKTYVVLIGILVLTVSMLYTAFLAAKLKEGEEKS
                     VQLYALAIQDLAHNSSTDNISDVSLQLTQDFKIPAILTDSKDEIIDAVNFGEHENRDT
                     VFLRKQLHEIKRSGYKPIEIENQFIRQRVYYKNSLIYTYLTYFPYVQLLLLAVFVGMG
                     YLGLSAARRSEQNRLWVGMAKETAHQLGTPISAIVAWIEHLKSMTEGNQDQTEVLNEL
                     GNDVKKLERVADRFSKIGSVPVLVPHNI"
BASE COUNT         1159 a          864 c          924 g         1228 t
ORIGIN      
        1 gtcaattatt gtcgtcgaat ccatgatata taccgttccg gtgacaatgg acgcgtatgt
       61 gtaaaagctg cggccatctt cttctatgtc aattttgaat tcaaccgttt caaagtcttt
      121 caaggctgct tcggcaacaa tcctaaaggt gatatcgagg ctgtctccgg acgcaatctc
      181 tccgggttgg atcgatgctg tcagtacatt aaccttagtg gcgttggtac ctatcggtgt
      241 gcaagtgacg atggatgacg aagacaggcc ggcatccaca ttaaataacc ggacattcaa
      301 atcaaattcc tcaccacctg taatataacc atcagcgtta ctatcattgt catagttcag
      361 gtaattggct gtcaatgaag gaccacgtcc tatcttataa gtcatgaccg ggcccttgtt
      421 actgaaatcc cacaatctta gtccacttgg aatatcagaa atccaccaac gtgatgatgg
      481 ggtggtgctg tcgttatatt ccgccattcg gcctttcctg aagcatgcac ccgaatggtc
      541 gttgatattg ttgttctggt ggacaacata tacctgatgg ttggtggttt gattatctcc
      601 gttttcattg atgcgccata caaccagacc ttcatcgggt attccctcac ttcttccggt
      661 tttaagacgg ttttgaaaca tataatactc cgttggaata tccggatttc tataggaata
      721 tgcggcatat gcattagcag tgtcagtaag gatttcattc tttcctgtga cgtctgtatc
      781 tctcaaccag ccaatcctat gcatgaagta agggttgggc aatgtcggat tagcatcggg
      841 tcctccggag cacatgacat cgaatgcgcc aatgccatcc ggaccggatt tgttgtcata
      901 tttgtatgta tcaggccatt tgcatatcat gtggccgttt tcgtgaacta ccgtaccgat
      961 ggtcaatgga gatttggcag gtgaggtatt gtatctgccc gatcttacac cattagcaga
     1021 gaaaccacta taatagccct gatgaaacca catgcccttg gcccatgatg gcgggttgcc
     1081 cgtgtacatc aggttgatag cctggatggt tttgtcggga ttggtggata gggtggagaa
     1141 gtcaaatcct gtacttttga tccaattcag tgccaggtcc aatatttgtt gggccccgac
     1201 agcataaggc atggcgtcat attcctggaa ggttctgggt gctctgaaaa ttccgaagac
     1261 gacattttga tagtcgagca gtcctcccga gatatccaga taatactgtc tcaaagatcc
     1321 attattacca aaccggacat agttaagacc gttacagaaa tcaatgtaat cctcactctt
     1381 taaggtagct acttcatccg aaaaatcaac aatgatgcaa agacccttca aatttccctg
     1441 caatacttca cctgatcgct ggtaggttgt gtaactattt tgaacggcct ctacggtagg
     1501 tagtccaaag agcgattgag cgttgtgatt catgacctcc tgcacatatt ccggacggat
     1561 atctaaatgt tttggtacac cgggatcgac attcagcgtt gcacctcctg attgagaacc
     1621 aaaatatctg tattcggtgg ctattaactc tctgccatcc tccgataatc gggcataaca
     1681 aatccattta ctggcatcat ccctgacaag ggtataacca tccaggcctt ccgcacgcat
     1741 ataaaattcg gagccgaaca gcctcacatc gacttgtgaa ccatcgggtt gggaaaaggt
     1801 ctttatttcg ccattataat tggaagaaaa tgcggttagt gaaagaagga aaagaaaaaa
     1861 agtctgaaaa aaacgcatct ggcaatcatt taatttagaa aaaataaaaa ttgaaaggta
     1921 aagatagaaa tgtatctatc aaattgaaag gatattcagt taatttgtaa tatagtggtg
     1981 tgcattgttt cctttcaccc aatgaatttg aaagcaatcg gaaggtcttt ccgggtgaaa
     2041 gaaagtgact cgtgtttcag agaaaatgat ttctgaacca ttgtccgatg tgccatcttg
     2101 tcaatgattg ttgaatgtca gtagcttggg tgtattgacc attatggaac accctgtttg
     2161 tatagctcct gtaatagtga atatatggca tcggctgttt tttcccagga gaatctcttg
     2221 atgttattaa atcccttctc aatcagttcc gctctcaata gactatcctc cattattttc
     2281 ttcatggcat cggctatagc gttgtcatcg gatggattta ccagtaaggc agctcctcct
     2341 gaggcttccg gcaaggatga agtgttggag gctattaccg ggcagccgca ccgctgtgct
     2401 tctatgaccg gcatgccgaa tccttcaaag taggatacac acaataaggc ggatgcgtga
     2461 ttgaggatat gggatatttc ctcatttgac cgataaccaa gcagttccac atcctgttta
     2521 taactatgtt tgtccaggat tgtatagaat tcttcatcat tccacccttt gcctccgatg
     2581 aatttcagtt ttgtcaacct tcctgttcta tttttatata attcaaaggc tcttaataga
     2641 ccgatgacgt tttttctggg ttggatggtg cccaggtgca cgaaatagtc ctgcctgaca
     2701 gtgattccct cgaactcaaa accctcattt aaacctaatc cgacgacttc gaccttgtcc
     2761 ggcgcaatat cgtattgttg aagaatgtct tgtcttgtgg cttcactgac agtggctatc
     2821 ctgtctgcac gacgtgcaaa cttaggcatg aaatacctgt aataaagcag ttgcattccc
     2881 ttaaatccat ccggggtatg gaggtaggcc aggtcgtgga tcacgagtaa ggtaggtagt
     2941 ctggtgcgta atgacaagaa gttgtccggg ctaaaaaaga catcggcttt actactccta
     3001 agaatggccg gtatactcca ttcaaaccag attgtaaaaa gtaaggttgc cctagccgga
     3061 ggatgaacaa taattggttt tatatttgga gcaaatatga aagagctgtc atagggtcgg
     3121 tcaaagagga agataaatgt atcatccgga tgattcaaga ccaattggcg aacaacttcc
     3181 agggtaaaat gacctagccc tgtcagctta ttttttaata aaaatcttgt attgacagca
     3241 atcctcattg taacgcaaag ctaatcagag tttactttaa ttatttaccc gggaggatat
     3301 tttatcacta agaaccaata cgaggtgctt gtggctacat ttaaaatgca aataaaacaa
     3361 aaataaagcc caatgataaa atatttcata catttggaat gttcataact cctggtaatt
     3421 aaacgttgat catcgtttgt tgaaaatatt cgatttcagg acatattgga agacttatgt
     3481 cgtgttgatt ggcatcctgg tgttgaccgt atcgatgctt tatactgctt ttttggccgc
     3541 caagctgaaa gagggtgagg agaagagtgt tcagttatac gctttggcta ttcaggacct
     3601 tgcacataat tcttccactg ataacattag tgacgtgtct cttcagctca cacaggactt
     3661 caagataccg gctattctta cggattctaa agatgagatt atagatgcgg tcaatttcgg
     3721 agagcatgaa aatagagata ccgtattttt aaggaagcaa ttgcatgaaa tcaaacgaag
     3781 tgggtacaag cctattgaga ttgaaaatca atttatccgt caaagggtat attacaaaaa
     3841 ttccctcata tatacttatc ttacttattt cccttatgtt cagttgttgt tgctggcggt
     3901 ttttgtcggc atgggatatc tcggcctgag tgcagcaagg cggtccgagc agaacaggtt
     3961 gtgggtaggt atggccaagg agacggcaca tcaactggga actccgattt cagctattgt
     4021 cgcctggatt gaacacctga agtccatgac cgaaggtaat caggaccaga cggaagtatt
     4081 gaatgaattg ggcaatgatg taaagaaact tgagcgagtg gcagacaggt tctccaagat
     4141 aggttctgtt ccggttcttg ttccgcataa tattt
//
LOCUS       DAOQQN010000298         4164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_10091_length_4164_cov_5.345096, whole genome shotgun sequence.
ACCESSION   DAOQQN010000298 DAOQQN010000000
VERSION     DAOQQN010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10091_length_4164_cov_5.345096"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2447
                     /locus_tag="PLG24_10500"
     CDS             <1..2447
                     /locus_tag="PLG24_10500"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMZ25364.1"
                     /translation="LVLGKIIDEISGEPVEAALIYEKSEQIGVESDVNGFFQLSVPAG
                     KPLTIIIRRVGYETAAIKLNSITEGTIKKLDISLIQKDAGQGVTIYAESIGDKNMVRA
                     NVKEFKLLPTVSGNLESVLPSIALGTSSGSGGELTSQYNVRGGNYDENLVYVNDFEIF
                     RPQLIRSGQQEGLSFPNIDLIRDLAFSSGGFEAKYGDKMSSVLDIKYKRPEERRGSVS
                     GSLLGATAHLEGLMKPFKNRGGQLRYLLGARYKTTKYILGSLDTKGEYQPNFIDLQAY
                     LTYDFHPDWQLAMIGNFNSADYNFTPTETEVVTGGFFFPHKFSADFEGGERDRFRNNM
                     AGLALNYVPQRRKNPSFVKFLTSVYNGDEIEAYDILSFFKVSKLELDLKGGKPKEIGV
                     LGDGIQHDFARNRLQNIIVNGEIKAGIEIPRFNDEKGQESSHFLYGGVKIQQEHFRDR
                     INEWSRTDSAGYSLPNSEEGYFLDGVLKSVNDIVSQRMTAFGQETYTFKKVGKYEVQA
                     TVGARAGYWSFNDEWIFSPRAQFLYRQLGKENLLTYKLAGGIYQQQPFYREMRRPDGT
                     LNQNIRSQKSAQVIGGITYEFTVGRKRPVRFKMITEAYYKDLWDQISYNVDNVRVRYS
                     GENDSRAYARGVDFRINGEFVNGFESWLNLSYLDTKEKINGVAHQDAFGNSVAWVPRP
                     TSQYFSASLYFQDYLPGNKDFRTHLQLNFGTGLPYGPPGENLIKRNALTFKPYQRVDI
                     GFSYQMWDVSKREEHPQHFLRWTQRTFISLEVFNLMDVQNVAAVNWVKDFNNINYYFP
                     INLTSRRINIKLRFDL"
     gene            complement(2783..>4164)
                     /locus_tag="PLG24_10505"
     CDS             complement(2783..>4164)
                     /locus_tag="PLG24_10505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ25365.1"
                     /translation="EDVSCHGLTDGSLIALANGGASGFSYQWSNGATTDMIGNLSAGT
                     YTVTVTDITNCTTQASATITEPAALSLTASITGVSCNGEDNGSITVMVSGGTFPYLFH
                     WNNGSNASSIANLSAGSYTVTVTDSKLCESIATYTITEPPLLNVHVATTDASSDVAAD
                     GTAEAIAEGGKAPYSYLWSTGATTPKITGLTAGNYSVTVTDANGCKKVSTITIQGKEC
                     LLTVVASGTSVTCAGLEDGSATAIPANNTGNAEYLWSNGATTQTISGLAPGGYSVIVT
                     DNIGCTATTTITITQPDTITLKIISITPSTTSTSQNGSISIEVTGGTPGYTFIWYSSG
                     KEVSTEQNPSNLNSGFYHVVIKDSHGCIHTSPEIFVDFLLSSNSTKQDPVRIFPNPAN
                     GFIYISYIDEYPGDISMYDILGRKVTLSITKEQNNVRLETSSLADGPYIIQIRMGRSV
                     YAARVVVHH"
BASE COUNT         1151 a          963 c          975 g         1075 t
ORIGIN      
        1 ctcttgtttt gggaaaaata attgatgaaa taagcgggga gcctgtcgaa gccgccttga
       61 tttatgaaaa gagcgaacaa atcggcgtag agagtgatgt aaatggcttt tttcaactat
      121 cggtgcctgc gggaaaacct ttaaccatca tcattcggag ggttgggtat gagacagcgg
      181 ccatcaaact taattcaatt accgaaggaa ccattaaaaa acttgatatt agtcttatcc
      241 agaaagacgc aggacagggg gttaccatat atgctgaatc cattggagac aaaaatatgg
      301 ttcgtgccaa tgtgaaagaa ttcaagctgc tccccaccgt ttccggcaat ctggagagcg
      361 tcctgccttc cattgccctg ggcacctcct ccggctcagg aggtgaattg acttcccaat
      421 acaatgttcg cggtggcaat tatgacgaaa acctggtcta tgtcaatgac tttgaaatat
      481 tccgtcctca gttgattcgc tccggccaac aggagggcct ttccttcccg aatattgatc
      541 tgatacgcga tctcgccttc agcagtggtg gcttcgaggc caaatacggg gataagatgt
      601 cctctgtcct cgatatcaaa tacaaaagac ctgaagagcg ccgtggctcg gtttccggca
      661 gcctgctggg agcaacagca cacctggagg gattgatgaa gccatttaaa aacaggggcg
      721 gacaattgag atatcttttg ggagcaaggt acaaaaccac caaatatatt cttggttccc
      781 tggatactaa aggagaatat caacccaact tcatcgacct gcaggcctat ttgacctatg
      841 acttccatcc cgactggcag ctagccatga tcggcaactt taactcagcc gattataatt
      901 tcactccaac ggaaaccgaa gtagtgacag gtggtttttt cttcccacat aagttcagcg
      961 cggatttcga aggtggagaa cgggatcggt ttaggaacaa tatggcaggg cttgcactaa
     1021 attacgtccc acaaagaaga aaaaatccat cttttgtcaa gttcctgacg tctgtctata
     1081 acggtgatga aatagaggca tacgatattc tttctttttt caaggtttcc aagctggaac
     1141 ttgacctcaa gggaggcaaa cccaaggaaa tcggtgtgct gggtgatggt atccaacatg
     1201 atttcgccag aaacagatta cagaacatca tcgtcaacgg tgagataaag gcgggaattg
     1261 aaatcccgag atttaatgac gaaaaaggtc aagaatcttc acattttctt tatggtggtg
     1321 tcaagatcca gcaggaacac ttccgtgacc gaatcaatga atggtccagg acggacagtg
     1381 ccggatactc cctgcccaac tccgaagaag gctacttcct ggatggcgtc ctcaaatcgg
     1441 tcaatgatat cgtatcacaa cgtatgaccg ctttcgggca ggagacctat accttcaaga
     1501 aagtaggcaa atacgaagta caggcaaccg tcggagcccg cgcaggatac tggagtttca
     1561 acgacgaatg gatattctca cccagagcgc aattcctgta cagacagctt ggaaaggaaa
     1621 acctactgac ctataaactg gccggaggaa tctatcaaca acagccattc taccgggaaa
     1681 tgcgccgacc ggatggtaca cttaaccaaa atatccgctc ccagaaatct gctcaggtca
     1741 tcggcggtat tacttatgaa tttactgtcg gcagaaaacg tcctgtgcgg ttcaagatga
     1801 ttacagaagc ctattacaag gacttgtggg atcagatatc ctacaatgta gacaatgtgc
     1861 gcgttaggta cagtggtgaa aatgacagcc gcgcttatgc tcgtggggtt gatttcagga
     1921 tcaatggcga atttgtaaat ggctttgaaa gctggctcaa tttgtcctac cttgatacaa
     1981 aagaaaaaat aaatggcgta gctcatcagg atgccttcgg caattccgtg gcatgggtgc
     2041 cccgaccgac atcacaatat ttctcggcat cactttattt tcaggattat cttccgggca
     2101 acaaagattt ccggacacac cttcagctta atttcggcac cggcctccca tacggacctc
     2161 cgggagagaa tctgatcaag agaaatgccc ttacattcaa accataccag cgcgtagata
     2221 ttggcttttc ctatcagatg tgggacgtat caaaaagaga agaacatcca caacacttct
     2281 tacgatggac gcaaagaaca ttcatcagtc tggaagtatt taaccttatg gacgtacaga
     2341 atgttgctgc agtcaactgg gtcaaggatt ttaacaacat taattattat tttccaatca
     2401 acctgaccag ccgaaggata aatatcaagc tgaggtttga cctctgacag tctgactttg
     2461 gcacaatatc atcgaacaat cctcatacct tggcgattga taatatcctc ccctcgggaa
     2521 aaagtataag cagccacaag cgccatctgc ttgatctgca tcagtaaaca tgagcacgag
     2581 tccaattatc cgatgactgg tacccgtcat ttcgatgtag tccattcata ggcatgacgt
     2641 taaagggaaa tctccacctt ttgaaaggca aaaaatggtc gattcatgtg agacaggtac
     2701 cgaatcattt gtgaattcag caactccttc cttgtctatg aattcagctc atcgcgggaa
     2761 gtgggactga tgtccaacaa catcaatgat gcaccaccac tctggctgca tatacactcc
     2821 gacccattct aatctgaatg atatatggcc cgtccgctaa ggacgatgtt tcaagccgga
     2881 cattattttg ttccttcgtt atgcttaaag taactttccg gccaagaata tcatacattg
     2941 atatatcgcc tggatactca tcaatataag atatatagat aaagccattt gccggattcg
     3001 ggaagattct gacaggatct tgcttggtcg aattggaact aaggaggaaa tccacaaaga
     3061 tttccggact cgtatggata catccatggc tatccttgat tacgacatgg taaaagccgg
     3121 aattgagatt ggatggattc tgctcggtag aaacctcctt gccggaagaa taccaaataa
     3181 atgtatatcc cggagttcca ccggtcactt cgatggagat gctgccattc tgtgacgtac
     3241 ttgtcgtgga cggcgttatt gatatgattt tcaaggtgat ggtgtccggt tgggtaatgg
     3301 ttattgtagt agtggccgta caacctatat tatcggtgac gattaccgag tatccaccgg
     3361 gtgccaggcc tgatattgtc tgagtggtag cgccattgga ccacaggtat tcagcatttc
     3421 cggtattgtt ggcgggaatg gccgtggcgg agccgtcttc aagtcccgca caagtaacag
     3481 atgtgccgga cgcaactact gtaagcagac attccttccc ctgtatcgtg atagtagata
     3541 cttttttaca accattggcg tcggtgacgg tcacagaata attgcctgca gtgagtccgg
     3601 taatttttgg cgttgtagct ccggtagacc acagataact atatggcgct ttgccccctt
     3661 ctgctattgc ctccgcagtt ccatcagctg ccacatcgga tgaggcatcc gtggttgcga
     3721 catggacgtt gagcagaggc ggctctgtaa ttgtgtaggt agctatgctc tcacatagtt
     3781 tcgaatcagt cacagtgact gtgtacgatc ctgcactgag gttggcaatg gaagatgcat
     3841 tgctgccgtt attccaatgg aacagatacg gaaaagtgcc ccctgaaacc atcactgtaa
     3901 tcgaaccatt gtcttctcca ttgcagctta ctcctgttat gcttgccgta aggctgagcg
     3961 cggccggctc tgttatggtc gctgatgctt gtgtcgtaca gttggtgata tctgtcaccg
     4021 tgacagtgta ggttcctgca gacagattgc cgatcatgtc agttgttgcg ccattactcc
     4081 actgatagct gaagcctgat gcaccaccat ttgcgagagc aatcaaagaa ccgtcagtca
     4141 ggccatggca ggacacatcc tcct
//
LOCUS       DAOQQN010000299         4135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_10217_length_4135_cov_5.826716, whole genome shotgun sequence.
ACCESSION   DAOQQN010000299 DAOQQN010000000
VERSION     DAOQQN010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10217_length_4135_cov_5.826716"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1411)
                     /locus_tag="PLG24_10510"
     CDS             complement(128..1411)
                     /locus_tag="PLG24_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HMZ25366.1"
                     /translation="MEIWIILGLMVLNGLFAMCEIALASSKKARLIERASKGNKNAVI
                     ALKLTENPDSFLAAMQVGVTTIGILAGVYGGQAFAKDLETYFSQISWLSTYAHSLALL
                     IIIGGITYLTIVVGELIPKKIGLMYSEEITLFMAPFVRVFTKIAYPIVWFLTTSTKLL
                     SKLLFIKDVETDIVSEDELKMMVKMANEDGTIDNKESELIHNIFRFADRKAYSIMTPR
                     NEVVWIDANESSAEIHSDIIESGLTKFPVCDDSLDNILGIVNLKDYLISRNRPGFDIK
                     NILQPVVLIPETLTSLKILEEFRKKKRYFAVVMNEHGYVEGVLTLHDLTENIFGSLPD
                     LEEEEEIMIFKREDGSYLIDGSCQIDELREQIYIDEFFSEEADYTTIAGFVIEKLGKM
                     PEIGDKFSNDSYSFEIVDLDRTKIDKVLVTRISEE"
     gene            1975..2658
                     /locus_tag="PLG24_10515"
     CDS             1975..2658
                     /locus_tag="PLG24_10515"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ25367.1"
                     /translation="MRQLVILVFSIGYIVLSGVSCKGDASVQEHSGFPDPVPNDSVTT
                     MTESDNEPDRVIWQKPDMILDLLGNLSDKVVADIGAGTGFFTFRLLQHAGKVIAVDID
                     KVPLDRMSQLSRKLDTAISNKLEIKLAKPNDPELKPASVDVIFMSNTYMYIQNRVPYL
                     KNLIQYLKPHARILIIDYKKKSIPFGPPIEERVDLGTVEEELMKAGYKIIKSDDVSLD
                     YQYIVLAER"
     gene            2760..3290
                     /locus_tag="PLG24_10520"
     CDS             2760..3290
                     /locus_tag="PLG24_10520"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HMZ25368.1"
                     /translation="MAGLDQFIIPVKGLHLGMHNYRFEVDDGFFKEKQSTVVESGKFE
                     VNVELDKHIDLMVLNISFSGKWRTSCDRCTADIAIPVDGQSEYLIKYATEESDDGDVI
                     YIMRESSEINVSDLILDTITVGMPLSRTYDCESERPKPCDDEVLGKLRHNANEDNISS
                     PGNPWSDLLGLLSKEE"
     gene            3320..3532
                     /gene="rpmF"
                     /locus_tag="PLG24_10525"
     CDS             3320..3532
                     /gene="rpmF"
                     /locus_tag="PLG24_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HMZ25369.1"
                     /translation="MPNPKWRHSKQRKRKRRTHDSLTLPTIGTDKTTGENHLMHRAHW
                     SEGALFYRGRKVIEAAAGKDVDFTEV"
     gene            3619..4002
                     /locus_tag="PLG24_10530"
     CDS             3619..4002
                     /locus_tag="PLG24_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002886926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HMZ25370.1"
                     /translation="MKKVIFTERAPAPIGPYSQAVEANGMLYVSGQIPIDPATGQLIT
                     SDIESETRCVLENVKAILEACNLTLDDVVKSSIFITDMNNFARINAIYAEYFSGDNPP
                     ARETVQVAALPRSVNIEISVIAVTQ"
     gene            4038..>4135
                     /locus_tag="PLG24_10535"
     CDS             4038..>4135
                     /locus_tag="PLG24_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25371.1"
                     /translation="MQRILSGIQPTGDLHLGNYFGAIKNWVTQQGK"
BASE COUNT         1233 a          799 c          825 g         1278 t
ORIGIN      
        1 attaatctat caatgagtta ataggtttgt gtaatccatt taaatctgac cggtaagatg
       61 tgtttcttac tgcgttattc cggccaataa ctttcctttt aagaactttc cgagacacca
      121 ggcgcgttta ttcctccgat atccgtgtta ctaagacctt atcaatcttg gttctgtcca
      181 aatccactat ttcaaagcta taactgtcat tggagaattt gtcgcctatc tcaggcattt
      241 tgcctaattt ctctatcaca aagcccgcaa tggtggtata atctgcctct tcactgaaaa
      301 attcatcaat gtatatttgt tccctcagct catctatctg gcaagatcca tcaatcaaat
      361 acgaaccatc ttctctctta aagatcatta tttcttcctc ctcctccaga tccggtaagc
      421 tgccgaaaat gttttcggtg agatcatgta gggtcagcac accttcaaca tagccatgtt
      481 cgttcatgac aacagcgaaa tacctttttt tctttctgaa ttcttccaat attttcagtg
      541 aggtgagcgt ctctggtatc aatactaccg gttgcaatat attcttgatg tcaaaacctg
      601 gtctgttccg gctgattagg taatctttta ggttgacaat gcccagaata ttatccaaac
      661 tgtcgtcaca aacgggaaac ttggtgaggc cgctttcgat aatgtcggag tgaatctcag
      721 cagaagattc gttggcatcg atccagacaa cttcattacg aggtgtcatg atactgtagg
      781 cttttctgtc ggcaaacctg aatatattat ggataagctc actctctttg ttatcaatgg
      841 taccatcttc attggccatt tttaccatca tcttgagctc atcttccgaa acgatgtcgg
      901 tttccacatc cttgataaaa agcagcttgg ataaaagttt ggttgaagtg gttaaaaacc
      961 agacaatagg ataagcgatt ttagtgaaga cccgaacaaa gggcgccata aacaaggtaa
     1021 tctcttcgga atacataagt ccgatcttct ttggaattaa ttctcctacc acaatagtga
     1081 ggtaggtgat tccaccgatg atgattagta atgccagact gtgagcatag gtactcaacc
     1141 aggatatttg cgaaaaataa gtttccaaat cctttgcaaa tgcctgccca ccatatacac
     1201 ctgccaaaat tccaatagtg gtcactccaa cctgcatggc agcaagaaag ctatcaggat
     1261 tttcagtaag ctttaaagcg atgacagcat ttttgttccc ctttgaagct ctttctatta
     1321 atcttgcttt tttggatgag gccaatgcga tctcacacat ggcaaacagg ccattaagaa
     1381 ccatcaatcc aagaataatc caaatttcca tttaaaagtg ttgacaaagg cctttcttac
     1441 atttatcaca tactacatgc ctttcagcgc aaaggtaact attttgcttt aatcatatat
     1501 ttattttcaa tgacacaatc ttttgacagc atcatggata gacatcattg tgtgaagcgt
     1561 atatttctgt tcttgatgat tgtgaaaatt gacatggatg cattttgttt ggatttatgg
     1621 catagctata taaaatgtat tcttaaaatt tctgagataa ttggagatta aattgtaaag
     1681 acaggtttat agatttttat atattaaata aaagcaatat attaatcata agctgtttat
     1741 agctgttata atattctccg gttatgttac ttcagaccct ttcacgcaca gttgctgcga
     1801 tcaggcgata tatatatcaa aaagtaatgc ccaaaatact gttagcagtg gagtagaatt
     1861 atgttttggt taaatagact tctgataaat gtgtaactca acaatgggct ttttgactgt
     1921 tattttaatc ttttgctaca ttttttgttt atacttgcag ttaaatattg agtgatgcga
     1981 caattagtca tattggtctt ttctattgga tatattgtct tgtccggcgt cagttgcaaa
     2041 ggggatgcat ccgtacagga acattccggt tttcctgatc ctgtaccaaa tgatagcgtc
     2101 actactatga cagaatcaga caatgaaccg gatagagtaa tctggcaaaa accggatatg
     2161 attctggatt tattgggcaa tctaagtgat aaggtcgtcg ctgatattgg cgcaggtacc
     2221 ggattcttta cctttaggtt gcttcagcat gccggcaagg taattgctgt tgatatagat
     2281 aaggtgcctc tcgataggat gagtcagctg tcaagaaagc tggatactgc tatttccaac
     2341 aaattggaaa ttaaacttgc caaacctaat gatcctgaac taaagcctgc aagtgtagac
     2401 gtaatcttca tgtctaatac ctatatgtat attcaaaaca gggtgcctta tttaaagaat
     2461 ctgattcaat atttaaagcc ccatgcccgt atcctgatta ttgactataa aaagaaatcc
     2521 atcccatttg gtccacctat cgaagaacgg gttgacctgg gtacggtgga agaagagttg
     2581 atgaaggccg gctacaagat catcaagtct gatgatgtgt cactggatta ccagtatata
     2641 gtacttgccg aaagataact tttgaattcg gttgaccttt aaaaaatgtg ttaaaaacat
     2701 tatttgaaat caaaaattaa acataccttt gcagtccatt tgtaaaagta tgaataggaa
     2761 tggccggttt agaccagttt atcataccag ttaaaggctt gcatctggga atgcataact
     2821 accgtttcga ggtggatgat gggtttttta aggagaagca aagtacggtt gtagaatccg
     2881 gcaaatttga agttaatgtt gaactggaca agcacatcga tctgatggtc ttgaatatat
     2941 cgttttccgg taagtggcgg acttcctgcg acagatgcac ggcagatatt gcgataccgg
     3001 tggatgggca atcggagtat ctcatcaagt atgccaccga agaatctgat gacggcgatg
     3061 taatttatat catgagagaa tcctccgaaa tcaatgtttc ggatttgatt ctagatacca
     3121 tcacggttgg tatgcctctc agcaggacat atgattgtga aagcgaaaga cctaaaccgt
     3181 gcgatgatga agttctggga aaactgagac ataatgctaa tgaagataat atttcttccc
     3241 cgggcaatcc ctggtctgat ttattgggct tgctgagcaa agaagaatga tttagttata
     3301 attataaact tattatacaa tgccaaatcc aaagtggaga cactctaagc agagaaaaag
     3361 gaaaagaagg actcacgata gtctgacatt gcctactata ggtaccgaca agactaccgg
     3421 cgaaaaccat ttgatgcacc gcgctcactg gtctgaaggc gctttattct atcgtggaag
     3481 aaaggtgatt gaagctgctg ccggtaaaga tgtagacttc acagaagttt aattaacatc
     3541 gtttagcaac caagatttta tcctgatctt cccaagaggt cgggtttttt cgtttatgta
     3601 aatcacaaat ttattttcat gaaaaaagtt atattcacag aaagggctcc cgctcccata
     3661 ggaccttata gccaggcagt tgaagcaaat ggaatgctgt atgtgtccgg ccagattccg
     3721 attgatccgg ctaccgggca gttaataaca agtgatattg agtccgaaac aagatgtgtt
     3781 ttggaaaatg taaaagccat cctcgaggca tgtaacctga cactggatga cgtggtgaag
     3841 tcttcaatat tcattaccga tatgaataac tttgcccgga tcaatgccat atatgcagag
     3901 tatttctccg gcgataatcc gcctgcaagg gagactgttc aggtggcagc attgccgagg
     3961 tcggtcaata tcgagatatc agttatcgca gtgacccaat aatattttgt gtttcaattt
     4021 aacgtttttt aatccttatg caacgtattc tttccggaat tcaacctacc ggcgatcttc
     4081 accttggcaa ttatttcggc gccattaaaa attgggtgac tcaacaaggc aaatt
//
LOCUS       DAOQQN010000300         4118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_10278_length_4118_cov_6.015998, whole genome shotgun sequence.
ACCESSION   DAOQQN010000300 DAOQQN010000000
VERSION     DAOQQN010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10278_length_4118_cov_6.015998"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PLG24_10540"
     CDS             <1..496
                     /locus_tag="PLG24_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HMZ25372.1"
                     /translation="EEVLKKNEQIKYIAGEIKDSTNAIYLGRGVNFPVALEGALKLKE
                     ISYIHAEGYPAAEMKHGPIALIDEKMPVIVIATNRAAYDKIVSNVQEVKARKGIVIAV
                     VNEGDVEISKLADYTIEIPETEDALSPLLSVIPLQLLSYHIALMRGCNVDQPRNLAKS
                     VTVE"
     gene            526..1209
                     /locus_tag="PLG24_10545"
     CDS             526..1209
                     /locus_tag="PLG24_10545"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HMZ25373.1"
                     /translation="MGSRTLEEREALEYNTDRDNLVLPEYGRYLQKLANSIKKIEDPE
                     EQRAYIEKSIDIIIQLFPESRSVDNYREKLWHDFYYMTDFQLQALPPSGIRPTFEDQQ
                     LRPEKVDYPVHKLDYKHYGYNVQVMIKKALKMEDGPVKTEFVEVIGAYMKLAYKTWNK
                     EHFVSDEMIRQDLFNMTRGALAIDEEKNLDVLVSSQIKKRFLPSSNQPQNQNSKKKKK
                     NQNKNKSRR"
     gene            1427..3295
                     /gene="mnmG"
                     /locus_tag="PLG24_10550"
     CDS             1427..3295
                     /gene="mnmG"
                     /locus_tag="PLG24_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HMZ25374.1"
                     /translation="MFPEYDVIVVGAGHAGCEAAVGAANMGCRVMLITMNMQTIAQMS
                     CNPAMGGVAKGQIVREIDALGGYSGIVTDHTMIQFRMLNLSKGPAMWSPRAQSDRMAF
                     AAKWREMLESNPLIDFWQEMVSGLIVKDKRVAGVRTQMGIEIHGKAVVLTNGTFLNGI
                     IHIGEKQFGGGRAGERAARGITEQLVELGFESGRMKTGTPPRIDGRSLHYDRMDIQPG
                     DDNPGRFSFTDTPQVRDQMPCHITYTNDKVHDILRTGFSKSPMFQGRIQGLGPRYCPS
                     IEDKIERFSDKERHQLFVEPEGRNTIEIYVNGFSSSLPEDVQYKALKLIPGFEDVRMF
                     RPGYAIEYDYFPPTQLKMSLETHLIHGLFFAGQINGTTGYEEAACQGLMAGINAARYI
                     RQEDPFVLTRSQAYIGVLIDDLINKGTNEPYRMFTSRAEFRILLRQDNADLRLTPLAS
                     GLGMAGLEQRMNRVRMKNSALEEIREWFRVISGDPDNLNPYLDSVNSAPLKQKIKLDQ
                     IITRPDVKIVGLAAHFPELKEFLDRYDPEIISLAETHMKYEAYIEKEMEMVHRVNRLE
                     EMKLSPDLNYSTISSLSLEAREKLNKHKPATLGQAGRISGINPADISVLLIHLKPG"
     gene            3504..>4118
                     /locus_tag="PLG24_10555"
     CDS             3504..>4118
                     /locus_tag="PLG24_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HMZ25375.1"
                     /translation="MRFLISFWFFILLATIADAQVIRNNPNSNHANKFEQLGTILPTP
                     NEYRSASGSPGPKYWQQRADYNIKCTLDEGKQHLTGTEVITYYNESPDPLPYLWMQLD
                     ENQHSNINNAGYQSESEMPRQLTDRDLRNYEANKKDNGFGFNITRVVDAMGKPISFTI
                     NKTMMRLNLSKPLKPGEKIVFTIDWNYNLVNRREQGGRGGYEYFP"
BASE COUNT         1299 a          787 c          938 g         1094 t
ORIGIN      
        1 cgaagaggtt cttaagaaaa atgaacagat aaagtacatt gccggtgaaa ttaaggacag
       61 taccaatgca atttatctag gacggggagt caacttcccg gttgcattgg aaggagccct
      121 caagttaaag gaaatttctt atatacatgc agagggatat cctgctgctg agatgaagca
      181 tggaccaata gccctgatcg atgagaaaat gccggtcatc gtcatcgcaa ctaatcgtgc
      241 agcctatgat aagatagtaa gtaatgtcca ggaagtaaag gcaagaaagg gaattgtaat
      301 cgctgtagtc aatgaaggtg atgttgagat atcaaaactg gcagattata ccatcgaaat
      361 tcctgaaacc gaagatgcat tatcccctct gttgtcggtc attccgttac agttgctatc
      421 ctatcacatc gccttgatgc gcggatgtaa tgtggatcaa cccagaaatc tggcaaagtc
      481 agttactgtt gaataatttt ttaacctaat gcctaaattg aatatatggg ctcaagaaca
      541 ttggaagaaa gagaagcttt agaatataat actgatcgcg acaatcttgt cctgcctgaa
      601 tatggcaggt atcttcaaaa actggccaac agcattaaaa agattgaaga tccggaagag
      661 caacgtgcct atattgaaaa atctatcgat atcataatcc aactttttcc tgaaagcaga
      721 agtgtggata actatcggga gaaactatgg catgatttct attacatgac ggattttcag
      781 ttgcaggcat tacctccgag tggtatcaga cctacttttg aagatcagca gcttagacca
      841 gagaaagttg attatccggt acataagctt gattataaac actatggata taatgttcag
      901 gtgatgatca aaaaggctct gaaaatggag gacggacctg taaaaacaga atttgtcgaa
      961 gtgattggtg cctatatgaa actggcttac aaaacctgga acaaggaaca cttcgttagc
     1021 gatgagatga tacgtcagga tcttttcaac atgacgagag gagcccttgc cattgatgaa
     1081 gagaagaatc tggatgtttt agtttcatct cagatcaaaa agagattcct cccttccagc
     1141 aaccagcctc aaaatcaaaa tagtaagaag aaaaagaaaa atcagaacaa gaataaaagc
     1201 aggaggtaag gtaaaggacc tgatttggat tgatggtaaa tgattgcctt attgaatcaa
     1261 tctctaccct ctgacattag acattctgac tagatacatg gctttcacaa aaaacgcctg
     1321 agaatgaatt aatatttaag ggttatcaac gtataaattt ctctaaacta aaaggtaaat
     1381 gtttttgtct ttcgattttt tcaagtggta ggttctatat aaaagtatgt ttcccgaata
     1441 tgatgtaata gtggtcggtg ccggtcatgc cggttgtgaa gctgcagtcg gcgcagccaa
     1501 tatgggctgt cgggtaatgc ttataaccat gaacatgcag actatagcgc agatgtcctg
     1561 caatccggct atgggaggcg tagctaaagg tcagatagtt agagagattg atgccctcgg
     1621 tgggtattcg ggtattgtta ctgaccatac catgattcag tttaggatgc tcaacctgtc
     1681 taaaggtcct gcgatgtgga gcccacgagc acaaagtgac cgtatggctt ttgcagcgaa
     1741 atggagagag atgcttgaat ctaatcccct tatcgatttt tggcaggaaa tggtgtctgg
     1801 gttgatagtt aaggataagc gtgtcgctgg tgtccgtaca cagatgggta ttgagattca
     1861 tgggaaggcc gtggtcttga ccaatggaac cttcctgaac ggtattatac atataggcga
     1921 gaaacaattt ggtggaggta gagctggcga aagagctgcc aggggaataa ccgaacaatt
     1981 ggtcgagctg ggttttgagt ccggtagaat gaaaaccgga acaccaccca ggattgatgg
     2041 tcggagtcta cattacgaca gaatggatat acagccgggt gatgataacc ccgggaggtt
     2101 ttcatttaca gatacccctc aggtcaggga tcaaatgcct tgtcacatta cctacaccaa
     2161 tgacaaagtg catgatattc tacggacagg tttcagtaaa tcacccatgt ttcaaggtcg
     2221 aatacaaggc cttggtccaa gatattgtcc ctccatcgag gataagatag aacgcttttc
     2281 agataaggaa agacatcagt tgtttgttga gccggagggc aggaatacaa ttgaaattta
     2341 tgttaacgga ttttcaagtt ctctgcctga agacgtacaa tataaggcat tgaagttgat
     2401 accgggtttt gaagatgtcc gcatgttccg tcctggctac gccatcgaat acgattattt
     2461 tccgccgacc cagcttaaga tgtcattgga aacacatctg atccatggct tgtttttcgc
     2521 cggccagatc aatggaacta caggctacga agaagccgcc tgccaaggcc ttatggctgg
     2581 aatcaacgcc gcacgctaca ttcgtcagga agatccattt gtcctgacca gatcgcaagc
     2641 ctatatcggt gtcctgatcg atgacctgat caataaaggc accaatgaac catacaggat
     2701 gtttacttcc agagcagaat tcaggattct gcttcgccag gataatgccg acctgagact
     2761 tactccactc gcttctggat tgggaatggc cggacttgaa caaaggatga acagggtgag
     2821 gatgaaaaat agtgcactgg aagaaatcag agaatggttc cgtgtaatca gtggtgatcc
     2881 ggacaatttg aatccttacc tcgattcggt gaattcggca ccattgaaac aaaaaatcaa
     2941 acttgaccag atcatcaccc ggcctgatgt aaagatagtt ggattggcgg cacacttccc
     3001 ggagcttaag gaatttctcg acaggtatga tcccgagata ataagtcttg ctgagacgca
     3061 catgaaatat gaggcctata tcgaaaaaga aatggaaatg gtgcatcgag tcaaccgact
     3121 tgaagaaatg aagctgagcc cggacttgaa ctattccacc atatcatccc tttcgcttga
     3181 agcaagggaa aaactcaata agcacaaacc tgccaccctc ggccaggcag gacggattag
     3241 cggcatcaat ccggcagata tttcagtctt gttgattcat ctgaaaccgg gctgatacct
     3301 ttttgtttta aaattactct catataccct cgatttataa tgactcaagc agtcaagaat
     3361 catcgtggta tagcttgttg tataacaaaa tgcacagaga ttcaagtaat tcatataatg
     3421 aaagtttaat ttagtagaaa atttctaccg ttttgtacat taaaaattat atttggggta
     3481 ttcaaataat taaataattt ataatgcgct ttcttatatc cttttggttt tttattttac
     3541 ttgccacgat agctgatgct caggttatcc gaaataatcc gaattctaat catgctaata
     3601 aattcgaaca attaggcact attctgccaa ctcccaatga ataccggtct gccagtggaa
     3661 gcccgggccc aaagtattgg cagcaaagag ctgattataa tatcaagtgt acattggatg
     3721 agggcaaaca acatctgacg ggcacggaag taattacgta ctataatgaa tcaccggatc
     3781 ctttgccata tctttggatg cagttggatg aaaatcagca tagcaacatt aacaatgcgg
     3841 gttatcaaag tgaaagcgaa atgcctaggc agcttacaga cagggatcta agaaattatg
     3901 aggcgaataa aaaagataat ggttttggct tcaatatcac aagagtggtg gatgctatgg
     3961 gaaaaccaat atcctttacc atcaataaga ccatgatgcg tctcaatctt tcaaagccac
     4021 ttaaaccggg agagaaaata gtttttacta tagattggaa ttacaatctt gtcaaccgaa
     4081 gagagcaagg tgggcgtggt ggttatgaat attttcct
//
LOCUS       DAOQQN010000301         4115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_10289_length_4115_cov_5.660837, whole genome shotgun sequence.
ACCESSION   DAOQQN010000301 DAOQQN010000000
VERSION     DAOQQN010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10289_length_4115_cov_5.660837"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2037
                     /locus_tag="PLG24_10560"
     CDS             <1..2037
                     /locus_tag="PLG24_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HMZ25376.1"
                     /translation="IIGLIISGVIVGPHGLKLLEKNSAIELFSTIGLLYIMFIAGLEL
                     DLNEFKANKNKSLLFGFFTFSIPLAIGFPVVYYFLDYNFNASFLTASMFATHTLVAYP
                     IVSRLGVSKNQAVAITVGGTILTDTAVLIILAVVMENSKGSLNQEFWIRLAISLAIFS
                     VIMFLLIPRIAKWFFRKLESEKHSHYIFVLSVVFFAAFLAELAGIEAIIGAFVAGLAL
                     NKLIPHSSALMNRIEFIGNSLFIPFFLISVGMLVDISVILSGPQALIIAATLSVVAIS
                     GKWIAALFTQLAFRYSGAQRQLIFGLSSAHAAATLAVILVGFNAGVLDENILNGTVIL
                     ILITCIIASFATEKASKKIILDSGNDSLDLDSSSNTNDEHILLPIANVEKMDQLLDFA
                     IFLKDAKSVNPISILTVVSNNDEAEINIKKARSKLEDFVKQASASETNISIITTIDHN
                     PGSGIARISKEIMSDIIVLGWPQKTGLIDKLIGEKVANIMNSTDKCAFICHIDKPTNT
                     HKRIIVAAPPLAEHEKGFEMWMRKVSKLAQEFSIPVHIYCNQNTGKMVEKCSKKIKTS
                     ANIQVILFDDWEDFLVLSRNIHQDDLIILVSSRRGAPSYMPVLESLPAKLEKHFKTNS
                     RIIIYPQQYNIEQSLEGFEDVNAETLNKGLETVQRIGRKIGGIFRKEESKVTSE"
     gene            2348..2767
                     /locus_tag="PLG24_10565"
     CDS             2348..2767
                     /locus_tag="PLG24_10565"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HMZ25377.1"
                     /translation="MKKHVANAIWKGSVDEGSGNITTRSKALNNAPYSKAGRFADGPG
                     TNPDELIAASHAGCFAMALSMFLGKEGYTPEALEVKAEVTMDPGKLQLVGSHLTLHAK
                     IPGISEAKLLECANTAKDNCPISKALNMPITLDVHLV"
     gene            2814..3152
                     /locus_tag="PLG24_10570"
     CDS             2814..3152
                     /locus_tag="PLG24_10570"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMZ25378.1"
                     /translation="MSEQHDMYPQATAENAKKKADLAPNQIETWRKFSRSVFKEGVLD
                     EKTKQLIAVAVAHVTQCPYCIKSHVPQAMKKGASKEEIMEAIWVAAEMRAGAAYAHAT
                     IALDAMDNEV"
     gene            complement(3308..3754)
                     /locus_tag="PLG24_10575"
     CDS             complement(3308..3754)
                     /locus_tag="PLG24_10575"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HMZ25379.1"
                     /translation="MFRLLSFVLLLAFCLPLAQAQSSKGDKILGLWLSETKEGKIQVY
                     RVGNKYYGKLVWSKSMYGPDGKTLNKDTKNSDKNLRNRNLKDLVLLKDLTYDGEEYSG
                     GTIYDPKNGSTYKCYMVLKGNDVLNIRGYIGISLIGRTSVWTRIKE"
     gene            3877..>4115
                     /gene="nadA"
                     /locus_tag="PLG24_10580"
     CDS             3877..>4115
                     /gene="nadA"
                     /locus_tag="PLG24_10580"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HMZ25380.1"
                     /translation="MTGITQEMIDQMPLKGYLDVEVDPQLDLVAEIQKLKQEKNAIIL
                     AHYYQDSEIQDVADYIGDSLGLSQQAAVTDADMIV"
BASE COUNT         1283 a          734 c          836 g         1262 t
ORIGIN      
        1 atcataggat tgattatttc cggagttatt gttggtccac atggattaaa acttcttgaa
       61 aagaattcag cgatagaatt attctcaacc attggtttgt tgtatatcat gtttattgcc
      121 gggcttgagc tggatttgaa tgaatttaaa gcaaataaaa acaagagtct tctttttgga
      181 ttttttacct tctctattcc tcttgcgatt ggatttccgg ttgtctatta ttttcttgat
      241 tacaatttca atgcaagttt tcttacagca agtatgtttg ctacacatac cctggttgcc
      301 tatccaatag tgagccggtt aggggtttca aaaaatcaag cagttgcaat cactgtgggg
      361 gggaccattc tgaccgatac cgccgtgttg attattttag ctgtagtcat ggaaaatagt
      421 aagggaagtc tcaatcagga gttttggatt aggcttgcaa tatcccttgc aattttctct
      481 gtgatcatgt ttttgctcat cccaagaata gcgaaatggt tttttcgtaa attggaaagt
      541 gaaaaacatt cacattatat ctttgtacta tctgttgttt tctttgctgc ctttctggca
      601 gaattggccg ggatagaagc cataatcgga gctttcgtag caggattggc actcaacaaa
      661 ctcatccccc attcttcagc cttgatgaac agaattgaat ttatcggtaa ttctcttttc
      721 ataccatttt tcctgatcag tgtcggtatg ttggtggata tcagtgtgat tctcagtggg
      781 ccacaggcgt tgatcattgc tgcaactcta tcggtagtag ccattagtgg taaatggatt
      841 gcagcgttat tcacccaatt agcattcaga tattccgggg ctcaacgcca attaatattt
      901 ggtcttagca gtgctcatgc tgcggctact cttgcagtaa ttttagtggg gtttaatgcc
      961 ggagtattag atgaaaatat acttaatggt acagtaatcc ttatattgat cacttgtatc
     1021 atcgcttctt ttgctactga gaaagcttcc aaaaaaatta ttctggattc aggcaatgat
     1081 agccttgatt tggattcatc cagcaacacc aatgacgaac atattttact tcctatcgct
     1141 aatgtggaaa aaatggacca attgcttgac tttgccattt ttcttaaaga tgccaaatcg
     1201 gtcaacccaa tttctatact tactgtcgtg tccaataatg atgaagcgga gatcaatatt
     1261 aaaaaagcca gaagtaaact ggaggacttt gtcaaacaag cttcggcatc cgaaacgaat
     1321 attagtatta ttactacgat agatcataat ccgggaagcg gaatcgcacg tatttcaaaa
     1381 gaaatcatgt ccgatattat agtattgggc tggccgcaaa aaacaggatt gattgacaag
     1441 cttatcggtg aaaaagttgc caatatcatg aacagtacag ataaatgtgc cttcatatgc
     1501 catatcgata aaccaaccaa tacccataaa aggataattg ttgcagctcc tcctttggct
     1561 gagcatgaaa agggatttga gatgtggatg cgcaaggtat ccaaacttgc ccaggaattc
     1621 agtatccctg ttcatattta ttgcaatcaa aataccggta aaatggtcga aaaatgcagt
     1681 aaaaaaataa agacatcagc caatattcag gtcattttgt ttgatgattg ggaagatttt
     1741 ctggtactgt cccggaatat ccatcaggat gacttgatta ttcttgtgtc ttccagaaga
     1801 ggagctcctt cctatatgcc cgtattagag agtcttcctg ccaaattgga aaagcatttc
     1861 aaaacaaata gcaggatcat aatttaccca cagcaatata atatagaaca gagtcttgaa
     1921 ggttttgaag atgtcaatgc cgaaactttg aataagggcc ttgaaaccgt gcaaagaatt
     1981 ggaaggaaaa tcggtggaat cttcaggaaa gaagagtcca aagtgacctc ggaataattg
     2041 ttgggtcatt tcttctagct ctttgtttaa tgtttgattg aatgagttct ttggatccag
     2101 ccttgggtac tatcccatag gattgtcata tttaaaaaat atgtattcaa gttactttcg
     2161 ttgaaaattt attgaaaata agtagatcgt cttttagccg ttttttatta tatctggtgg
     2221 taaagatttt gtctttaaat attcaaagta actaatttca tgaacaaatt ttatattgat
     2281 ttattctaaa taagatttgt gtgcatcaaa ttaagctcaa ataatttaaa tcataaaaaa
     2341 tcagataatg aaaaagcatg tagcaaacgc aatttggaaa ggcagtgtgg atgaagggtc
     2401 aggaaatatc accacacgca gtaaggcctt gaacaatgca ccttactcca aggcaggcag
     2461 atttgcagat ggtcccggaa ccaatccgga tgaattaatt gcagcatccc atgcaggctg
     2521 ttttgcaatg gctctaagta tgtttttggg taaagaaggt tatacaccgg aggcattaga
     2581 ggtaaaagca gaggtaacca tggatccggg caaactgcaa ttggttggct cccatttgac
     2641 acttcatgct aagattcccg gtatcagtga agcaaaactt ttggaatgtg ccaatactgc
     2701 aaaagataac tgtcctatca gtaaggcgct aaatatgccg ataacgcttg atgttcatct
     2761 tgtttaatgg ctgacaggca ttctaataat agtaaaatca aaatagaaaa taaatgagtg
     2821 aacagcatga tatgtaccct caggcaaccg cagaaaatgc aaagaagaaa gccgaccttg
     2881 cgccaaatca aatagaaacc tggcgtaaat tcagccgatc agtgtttaag gaaggtgttt
     2941 tggatgagaa aacaaaacaa ctcattgcag ttgcagtagc ccatgtaacc caatgtcctt
     3001 attgtatcaa gtctcatgtg cctcaggcaa tgaaaaaagg ggccagcaag gaagaaatca
     3061 tggaagctat atgggttgct gctgaaatgc gggcaggggc cgcatatgcg cacgcgacca
     3121 ttgcattgga tgcgatggat aatgaagtct gactgaatgt ataatttaat cgtaaataga
     3181 ctttgacaaa ggtcagacag ggctcagagc acggtcgtga tttgatatca tgcgtgacgt
     3241 actttttttg agtaacaagg gaaaaggaat ttaatccttt tcccttgttg tttcaggaaa
     3301 agataactta ttctttgatt ctagtccaga ctgaagtcct gccaattaga gaaattccga
     3361 tatagcccct gatattcaat acatcattcc ctttcaacac catgtagcat ttgtaggtag
     3421 aaccattttt aggatcataa atagtacctc ctgaatattc ctctccatca taggttagat
     3481 cttttaataa gacgagatcc ttcaaattac gatttctgag atttttatca gaattttttg
     3541 tgtctttatt caaggtttta ccatcagggc catacatgct cttactccaa accagcttgc
     3601 cataatactt atttccgaca cggtaaacct gaatcttgcc ttctttagtt tcactgagcc
     3661 acagccccag gatcttatcc cctttggaag actgggcttg agcaagagga agacaaaatg
     3721 ccaataaaag gacgaatgat aataatctga acatataagt taatatttag cactaatata
     3781 aatattaaac gcaattcaaa agagaaaact tattttgtca aaattttgtt gcttttaacg
     3841 gatatttgcg tcttaaatag tgaaataaat tataaaatga ccggtataac tcaggaaatg
     3901 attgatcaaa tgcccttaaa aggttatttg gatgtagaag tggatccaca gttggatttg
     3961 gtggctgaga ttcagaagtt gaagcaggag aagaacgcca ttatcctggc tcattattat
     4021 caggattcag agattcagga tgttgccgat tatattgggg attctcttgg actgtcacaa
     4081 caggcagccg tgactgacgc agatatgata gtatt
//
LOCUS       DAOQQN010000302         4109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_10310_length_4109_cov_4.808091, whole genome shotgun sequence.
ACCESSION   DAOQQN010000302 DAOQQN010000000
VERSION     DAOQQN010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10310_length_4109_cov_4.808091"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1572)
                     /locus_tag="PLG24_10585"
     CDS             complement(<1..1572)
                     /locus_tag="PLG24_10585"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMZ25381.1"
                     /translation="MKNIILSLLCLLMLIDSSFGQSPAISPDSLAGSNHAGRNWWDVQ
                     HYNLAIIPAIQDSTLTGTVTITYKVLSEGAGMMIDLQAPLKIYKVLSGNQKVSYRHDG
                     RFWWIAKDGKGKVGSTDAITIFYEGKPRVAVKPPWDGGIDWNHDAKGRPWISTACQGI
                     GASIWWPCKDIQSDEPEQGADLFYTIPADLSAIANGKLVSVTDTKNGKRTWHWRVTQP
                     INNYNISMNIGHYEKIQDTFHGKQGNLDVEFFVLDYNLEKGKKLFPEIFSMLNCFEEK
                     VGPYPFYEDGYKMIETAHLGMEHQSGIAYGNNFQRGYKGTDRSKTGVGLTWDFILVHE
                     SGHEWFGNSITTEDVNDGWIHEGFTTYMETIYTECLSGKEDAQKYVIGQRHIITNHKP
                     MINQYGVNYDAPGDIYDKGASLIHTIRTIIDDDKKFYAILQGKARKYYHSIVKSNDIE
                     DYWILSTGFNLKPIFDQYLRTTMIPTLTYTINDDAMTLRWTNVVKGFKMPVILFLKDG
                     SPVRINVSEDPVSVKL"
     gene            complement(1621..2901)
                     /locus_tag="PLG24_10590"
     CDS             complement(1621..2901)
                     /locus_tag="PLG24_10590"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ25382.1"
                     /translation="MKVTTAWTFYDWANSAFSLVITSAIFPSYFLNVTVPVLHIGNIT
                     MSNSSFYAYIIATAYLFVAIMSPFASGFADSANNKKMMMLLFTIIGSFNCFLLFFFDG
                     MSNLQFGATAFIFALIGFIGSIVFYNAFLPEITTEDRFDSLSARGFSMGYIGSVILLL
                     VNLALITYPDTFGLSSSGQAARVSFAMVGIWWIGFATIPLHYLPGSFRLQLKRSKIAE
                     GLYILKDVWYKLRHLPAIKHYLTAFFFYNAGVQTVMLLAATFAKKELNFSTSDLIIII
                     LILQLVAVVGAIFFSKLSGWRSNKFSLMTMLLIWVAICVTGYLVVTKTQFYILAATVG
                     LVMGGIQSLSRSTYSKLIYTHQGETTSFFSFYDVVDKISTLLGTFTFGFIENVTDNMR
                     NSIFALGLFFICGIALLAITNLSDTNKNSMSEAL"
     gene            complement(3287..>4109)
                     /locus_tag="PLG24_10595"
     CDS             complement(3287..>4109)
                     /locus_tag="PLG24_10595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ25383.1"
                     /translation="GTTDSTRIIGNVIIGDRDLTVVGGISVSSLVGVENRFVIMNNLV
                     SDNRYGITTAGNSTGYIIGNIIENNNTETNPLVGGSGISLYNTNMVIISGNQIRNNLW
                     GITLIDKTVANLGSDDPEDYNPGGNIFSNNGNEGQTYALYNNTENPVKALHNCWIEDH
                     ESNVEEVEAVIVHKADDEKLGEVTFDPFECGITNSVRAIENGKINIYPNPSSGIVHFN
                     TRDAGFIQIYEISGHVLMHRKIVEETTLEIRLNPGIYFVKFEGKNSFSTGKLMIR"
BASE COUNT         1159 a          851 c          852 g         1247 t
ORIGIN      
        1 cagctttacg gatacaggat cttccgatac atttatcctt acaggactac catcttttag
       61 aaacaaaatc accggcattt tgaacccctt aacgacattg gtccaacgaa gcgtcatagc
      121 atcgtcattg atggtataag tcagtgtggg tatcattgtt gtcctgagat attggtcgaa
      181 aatgggtttt aggttgaagc cggtcgataa gatccaataa tcttctatgt catttgactt
      241 cacgatactg tggtaatact tgcgcgcctt accttgaaga attgcataaa acttcttatc
      301 atcatcgata atagttctga tcgtgtggat caggctggcc cctttgtcat atatatcgcc
      361 tggtgcgtcg taattgacac catattgatt gatcatgggc ttgtggttgg ttatgatgtg
      421 gcgttggcca atgacatact tttgtgcatc ttccttcccg gaaagacatt cagtgtatat
      481 ggtctccata taagtggtaa aaccttcatg tatccaccca tcgttgacat cttctgtagt
      541 gatactgtta ccaaaccatt cgtggcctga ttcgtgtaca aggataaagt cccacgtcaa
      601 tccgacaccg gttttacttc ggtcagttcc cttataacct cgctggaagt tgttgccata
      661 tgcgattccg ctctggtgct ccatccccaa atgggcagtt tctatcattt tgtaaccgtc
      721 ttcataaaaa ggataggggc caaccttctc ttcaaagcag ttcaacatgg agaatatctc
      781 aggaaaaagc ttcttgccct tctcgaggtt gtaatccaac acaaagaatt cgacatcaag
      841 gttaccttgc tttccatgga atgtatcctg gattttttca tagtggccta tattcataga
      901 gatgttatag ttgttgattg gctgtgtcac acgccagtgc caggttcgtt taccattttt
      961 ggtatcagtg acacttacaa gctttccatt ggcgatggcg gacaagtcag ccggaatagt
     1021 atagaaaagg tccgctccct gttcgggttc atcactttgg atatctttac atggccacca
     1081 aatactggca ccaatacctt gacaggctgt actaatccat ggtcggccct ttgcatcatg
     1141 attccagtct attcctccgt cccatggtgg tttgacagct actctgggct tgccttcata
     1201 aaatatggtg attgcatcag ttgatccaac cttaccttta ccatccttcg ctatccacca
     1261 gaaacggcca tcatgccgat agcttacctt ttgattccca ctcaatacct tgtatatctt
     1321 gagtggtgct tgcagatcga tcatcatacc ggcaccttcc gaaagtacct tgtaagttat
     1381 ggtgacagtt ccggtaagtg tgctgtcttg tattgcggga atgatagcca gattgtaatg
     1441 ttgtacatcc caccagttac ggccggcatg attggagccg gcaaggctgt caggcgagat
     1501 ggccggtgat tgtccgaatg aagaatcgat aagcattaaa aggcagagta gagaaagaat
     1561 gatatttttc atcgttatta attgaatgaa tttttctaaa gataaaatga atccttaaat
     1621 ctacaatgcc tcagacatag aatttttatt agtatccgat aaattggtga tggccaataa
     1681 tgcaattcca cagataaaaa ataggcccag agcaaatata ctgttacgca tattatccgt
     1741 cacattttct atgaagccga aagtaaatgt tccaagtaac gtggagatct tatccacaac
     1801 atcataaaaa ctgaaaaaac tggttgtctc tccctgatgt gtgtatatca acttggagta
     1861 agtcgaccgg gaaagactct gtatgcctcc catcactaac ccaactgtcg ctgccagaat
     1921 atagaattgt gtttttgtca ctactaaata accggttaca cagatggcta cccagatcaa
     1981 taacatggtc atcagtgaga atttgttgct ccgccaaccg gacagctttg aaaaaaatat
     2041 tgcaccgact acggccacaa gttgcaatat tagtatgatg atgatgagat cggatgtaga
     2101 gaaattcagt tctttcttag caaatgtggc tgcaagaagc atgacagtct gtacacctgc
     2161 attgtagaag aaaaaggcag taagatagtg tttgatggca ggaagatgtc ttagcttgta
     2221 ccagacatct ttgaggatat atagcccttc cgctattttt gaacgtttca gttgaaggcg
     2281 aaagctaccg ggcagataat gtaatggtat ggtggcaaag ccgatccacc aaattcccac
     2341 catggcaaaa cttactctgg ctgcttgtcc tgaggatgag agaccgaaag tatcgggata
     2401 ggttattaag gcaaggttga ctaataggag tatgacactt ccgatatacc ccatcgaaaa
     2461 gccgcgtgca gaaagggaat caaagcggtc ttccgtggtg atctccggca ggaaagcatt
     2521 gtagaaaaca atactgccaa taaatccgat taaggcaaat atgaaggccg tagcaccaaa
     2581 ttgtaaattg gacattccat cgaaaaaaaa caacaggaaa caattgaaag aaccaatgat
     2641 agtaaacaaa agcatcatca tctttttatt atttgcacta tctgcaaagc ctgatgcaaa
     2701 cggactcatg atagcaacaa agagataggc agtcgctatg atgtatgcgt aaaatgagct
     2761 gttactcatc gttatgttgc ctatatgcag gactgggacg gttacattta ggaaataact
     2821 gggaaaaatg gctgaggtta taactaatga aaacgctgaa tttgcccaat cataaaatgt
     2881 ccatgcggtc gtaaccttca tcttcgtttt gttttgaaat gtcttttcga agatagaact
     2941 ttatttctca atatgccgaa ttatccgata aatattttag tggtaaactt gtcgattgtc
     3001 gtcagtgact gcttccttta ttccttgctt ttttgtcgta tttgaacaaa agagttcagc
     3061 agtatcatgt ttgaatggta ttatgtagga tgcttagtcc ttattgaata aaagtaaaac
     3121 catattcaat tcaaatttta ttgactttaa ccatccattg acctgaaatt gaacatccat
     3181 tttagggaag atgcctgtca tgatgataaa gttcaatatc aatctaatgg ggtgatcttc
     3241 cgattccttg tcccggaccg ttacaatcct ggcttaatgc ctaacattac cggatcatta
     3301 atttgccggt ggaaaaacta ttcttgccct caaattttac aaagtagatt ccgggattaa
     3361 gcctgatttc aagggtggtt tcttcgacta tcttcctatg catcaacaca tggccactta
     3421 tttcataaat ttggataaat ccagcatcac gtgtattaaa gtggactatt cccgaggagg
     3481 gattgggata tatattaatc ttgccatttt cgatagcccg gacggaattt gttatgccac
     3541 attcaaatgg gtcgaaagtt acctccccta atttttcgtc atcagccttg tgtacgataa
     3601 ctgcttccac ttcttctacg ttggattcat ggtcttctat ccaacaattg tgcaaagcct
     3661 tgaccgggtt ttcagtattg ttgtacaaag cataggtttg accttcgttg ccattgttac
     3721 tgaatatatt gccgcctgga ttgtaatcct ccggatcgtc acttcccaga ttagccactg
     3781 ttttgtcgat tagtgtaatg ccccaaagat tgttacgaat ctggttgcct gaaatgatga
     3841 ccatattggt attgtataat gatatgccac tgccgccgac gaggggatta gtctcggtgt
     3901 tgttattttc aatgatattg ccgatgatat agcctgttga gtttccggct gtggttatac
     3961 cataacggtt gtcactgaca aggttgttca ttatgacaaa gcggttttca accccgacaa
     4021 gactggaaac ggatattcca ccaacgacag ttaggtcgcg atcaccaatg atgacgttgc
     4081 caataattct ggtagagtca gttgttcca
//
LOCUS       DAOQQN010000303         4105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_10333_length_4105_cov_6.047160, whole genome shotgun sequence.
ACCESSION   DAOQQN010000303 DAOQQN010000000
VERSION     DAOQQN010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10333_length_4105_cov_6.047160"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1140)
                     /locus_tag="PLG24_10600"
     CDS             complement(7..1140)
                     /locus_tag="PLG24_10600"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HMZ25384.1"
                     /translation="MTKNSTILLFILICFCVNIFAQVDSIPEFNVQKSADSAHIQLFR
                     ENSSAIDTLPPVITQKGIAKIATQQDLDDIVEYGSRDSTELDKKNQILHLWGDAYVNY
                     QTSKINAHYIRIDLKNNIATAELGHDAKGRPIGKPAVDLEGQQVVANKLSFNFKTKKG
                     IIHDGRFKQSDLYINGSKIKVIAGPYDSLRTDDVLYQEDALITTCNHPDAHFGIQASK
                     MKIVPDRVAVIGPSYVRIGGVPTPLVMPFGFFPLKKTKQTGIIFPNDYNYSPQWGYGL
                     KNIGYYLPVNDRMDLIFSGDVYLRGSHRIGVTTRYNRRYKYNGSVMLEYSTYYNEPSE
                     TYKVIRQTSYHLQINHNQDPKANPYNKFGGNANIQFFVQKCFS"
     gene            1278..2684
                     /locus_tag="PLG24_10605"
     CDS             1278..2684
                     /locus_tag="PLG24_10605"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HMZ25385.1"
                     /translation="MSSLRFSFKGTVKLMVTMFAILSFPFLSKADCPHHNEVHACTHS
                     KGITNYGFTSTGMSVLKFSKNLSQSDLESIINLLPEGHRQYKIRTIVLDAGHGGKDGG
                     CLGKDSREKNLTLEYVLALGAKIEKAYPDINVIYTRKTDEFIELHERANIANRAKADL
                     FISIHCNWNPNSNPYGTETYVLGLHRAKDNLEVAKRENSSIFLENDYQKNYDGFDPNS
                     PEGNILLSMSQNAHLTQSISIAEKIEHEFENTSGRKSRGVRQAGFLVLRATSMPAILI
                     ETGFLSNVNEESYMTSSKGKKEIVDNIFRGFERYKSNVEFSSGGEEKILADNSGNKNK
                     KQEEGVIESQKKVVVNETTNPVASDKTKNLSIINNLVDTNNDNSPEKVEICVQLEVTK
                     TIQDLSLAKWSPFSNIITRSENNLLKYQVVCTSIEEARSVKQVAKNQGFPQAFICAYR
                     DGERISLEEINEHKIGLR"
     gene            complement(2708..3355)
                     /gene="nth"
                     /locus_tag="PLG24_10610"
     CDS             complement(2708..3355)
                     /gene="nth"
                     /locus_tag="PLG24_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HMZ25386.1"
                     /translation="MAKKISKKQAKADDIRQILDDLFPEVPVPLHHKDVYTLLIAVLL
                     SAQCTDERVNKITPILFAKADNPADMVALGEDAIREIIRPCGLSPMKAKAIYNLSKIL
                     MDQYNAHVPCSFEALEALPGVGHKTASVVMSQGFGIPAFPVDTHIHRLAYRWGLSSGK
                     SVEQTEKDLKKLFPEAAWNKLHLQIIYFGRSYCKARPHIAADCPICNKYGRKGLD"
     gene            complement(3360..>4105)
                     /locus_tag="PLG24_10615"
     CDS             complement(3360..>4105)
                     /locus_tag="PLG24_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HMZ25387.1"
                     /translation="ADWLAVFMSGHRAMVKCSDKDSVLFPAILERLKEWAPEFSGNTI
                     IEERLKGYDAIIATGSNNTSLYFKSYFSHVPHIIRKNRTSVGVIQSGDDEEVLEAFNA
                     DIHSYFGLGCRNVSKLYLPLGFDIRLLMSVFERESSNIAFNKYKNNFDYNLSIYLLNK
                     IPFYTDNNIILIEDKSPFSRIACLHYEWYDDVNEVMTDIKSRRDDIQCIVSSDRVGDL
                     KVIPPGTTQSPGLFDYADDVDTMHFLSNI"
BASE COUNT         1280 a          841 c          819 g         1165 t
ORIGIN      
        1 ataaccttaa gaaaagcatt tctgaacaaa aaactgaatg ttggcatttc ctccgaactt
       61 gttgtatggg ttggctttag gatcctgatt gtgattgatc tggaggtggt aggaagtctg
      121 ccgaatcacc ttgtatgttt ctgaaggctc attataatag gttgaatatt ccaacatcac
      181 ggaaccattg tatttatacc ttcgattata tctggtggtc acacctatcc tgtgagagcc
      241 tctcagatag acatctcctg aaaagatcag gtccatccta tcgtttaccg gcaaataata
      301 accgatattc ttaagtccgt agccccattg tggcgaatag ttataatcat ttggaaaaat
      361 aatcccggtt tgctttgtct ttttcagtgg aaaaaaaccg aaaggcatga ccaaaggggt
      421 gggcacacca ccaatacgca cataagaggg acctattact gccacacgat ccggaactat
      481 cttcatcttg ctggcctgaa taccgaaatg agcatcagga tggttgcacg tcgtgatcag
      541 ggcatcttcc tggtacagga catcatcggt gcgaaggctg tcataaggac ctgcaattac
      601 tttaatttta gagccattga tatacaagtc actctgctta aaccgaccgt catggatgat
      661 accttttttg gtcttgaaat tgaatgacag cttattagcg acaacctgct gcccttccag
      721 atccactgca ggcttaccga tcggtcggcc tttggcatca tgacccaatt ctgcggtggc
      781 gatgttattc ttgaggtcaa tgcggatata atgtgcattg atcttgctag tctgataatt
      841 aacgtaagca tctccccata gatgcagtat ctgattcttc ttatccagtt cagtactatc
      901 cctggaacca tattcaacga tatcatccag gtcttgttgg gttgcgatct ttgcaatccc
      961 tttttgagta atgacaggcg gcaatgtgtc aatagcagaa gaattttccc taaataactg
     1021 aatatgagca ctatctgctg atttttgaac gttaaattca ggtatggaat caacctgtgc
     1081 aaaaatattt acacaaaaac atattaaaat aaataataat atggtagaat ttttggtcaa
     1141 ttcagacagc tactttatat ttgtgacgaa tttttataat ttataattaa ttgatattaa
     1201 tgcaatttta atttataata aaaattaata tattaataat attgaaacac aaagctaaac
     1261 aaaaaaccaa atagcatatg agtagcttaa gattttcatt caaaggtaca gtaaagctaa
     1321 tggtaaccat gtttgccatc ctttcttttc cattcctttc aaaggctgat tgccctcatc
     1381 ataatgaagt acacgcctgt acgcattcga aaggaataac gaactacgga ttcacatcaa
     1441 cgggaatgtc cgtgttaaaa ttttctaaaa acttatccca aagcgatttg gaatccatca
     1501 ttaatttgtt gcctgaaggt cacaggcagt acaaaatccg caccatagtg ctggatgccg
     1561 ggcatggggg taaggatggg ggttgtttgg gtaaggactc cagagaaaaa aaccttacat
     1621 tggagtatgt acttgcattg ggtgctaaga tcgaaaaggc ctacccggat atcaatgtca
     1681 tttatacaag aaaaacagat gaattcattg aactccatga gcgagccaat atagccaaca
     1741 gagcgaaagc ggatttattt atcagtattc attgcaactg gaatccaaat tctaatcctt
     1801 atgggactga gacttatgtt ctaggccttc accgggctaa ggacaacctt gaagttgcaa
     1861 agcgggaaaa ctcttccatt tttctggaaa atgactacca gaaaaactat gatggcttcg
     1921 atccaaatag tcctgaaggg aatattttac tttccatgag tcaaaatgcg catttgaccc
     1981 aaagtatttc cattgcagag aaaatagaac acgagtttga aaatacttca ggcagaaaat
     2041 cccgtggtgt gcgtcaggca ggattcctgg tattgcgtgc cacatccatg cctgcgattt
     2101 tgatagaaac cggcttcctg agcaatgtaa atgaagaatc ttatatgacc agctctaaag
     2161 gcaaaaaaga aatcgtcgat aacattttca gaggatttga aagatataag tcaaatgttg
     2221 aattttcttc cggtggtgaa gagaaaattc tcgctgacaa ttcaggaaat aaaaataaaa
     2281 aacaggagga aggggtaatt gagtcgcaaa agaaggttgt tgtaaatgaa actacaaatc
     2341 cggtagcttc cgacaagacc aaaaatctgt ctattattaa taacctggta gataccaaca
     2401 acgacaacag tccggaaaaa gtagagatat gtgttcaact tgaggtcact aaaacaatcc
     2461 aggatctatc ccttgcaaaa tggtctccat ttagcaatat aataacaaga agtgaaaaca
     2521 acctacttaa atatcaggtt gtatgcacat caatagagga agccaggtct gtcaagcagg
     2581 ttgccaaaaa ccaaggcttc cctcaagcat ttatctgtgc ttatagagac ggggaacgta
     2641 tcagtctgga agagatcaat gagcataaga taggcctccg atagaacctg atccgggttg
     2701 agcattatca atccagacct tttcgtccgt atttattgca aataggacaa tctgcagcta
     2761 tatgcggcct tgccttgcaa taagaacggc caaaatagat gatctgaaga tgcagtttat
     2821 tccatgccgc ttcagggaat agctttttga gatccttttc tgtttgttcg actgattttc
     2881 cggaagaaag tccccaacga tatgccagcc gatggatatg tgtatcaaca ggaaaagccg
     2941 gaataccgaa tccctgactc atcaccaccg atgcagtctt atggccaaca ccgggcaatg
     3001 cctccaatgc ctcgaaacta catggcacat gggcgttata ctgatccatt aatatctttg
     3061 aaaggttgta aatagccttt gctttcatgg gagaaagacc acatggacgt ataatttccc
     3121 ggattgcatc ctcgccaagg gctaccatat cggcaggatt atctgccttg gcaaacagga
     3181 ttggagtaat tttattgaca cgttcatcag tacattgtgc agatagcaaa acagcaatca
     3241 acaacgtata gacatcttta tgatgcagcg gtacaggcac ctcagggaat aaatcatcca
     3301 gaatctgtct gatatcatcc gcttttgcct gtttttttga aatctttttg gccatcgatt
     3361 cagatattac ttaaaaaatg catagtatcc acatcatcag cgtaatcaaa caagccggga
     3421 ctttgggtag tacccggcgg tatgactttc agatccccca ccctgtctga agacacaata
     3481 cattggatat catccctacg cgacttgatg tctgtcatca cttcatttac atcatcatac
     3541 cactcgtagt gcaggcaagc aatccgacta aaaggactct tgtcttcaat caatatgatg
     3601 ttgttgtcgg tatagaatgg aattttattc aataaataga tggacagatt atagtcaaag
     3661 ttgttcttgt atttgttgaa agcaatgttt gacgactctc gttcaaatac tgacattagt
     3721 agacgtatat caaaacctaa gggcaaataa agcttggata cattgcgaca gcccaaccca
     3781 aagtacgaat ggatatcagc attaaaagct tccagcactt cttcatcatc accggattga
     3841 atcactccta cactggttcg gtttttccgt ataatgtgtg gtacatggct gaaataactt
     3901 ttgaaatata aggacgtatt gttgctaccg gttgctatga tcgcatcata gcctttgagt
     3961 cgttcctcaa tgatagtatt ccctgagaat tcgggtgccc attcttttag cctttccaat
     4021 atagccggaa acagcacact gtccttatca ctacatttga ccatggcccg atggccggac
     4081 atgaaaacag ccagccagtc agcaa
//
LOCUS       DAOQQN010000304         4037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_10625_length_4037_cov_5.425665, whole genome shotgun sequence.
ACCESSION   DAOQQN010000304 DAOQQN010000000
VERSION     DAOQQN010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10625_length_4037_cov_5.425665"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1029)
                     /locus_tag="PLG24_10620"
     CDS             complement(139..1029)
                     /locus_tag="PLG24_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ25388.1"
                     /translation="MENVLEINNLTKRFGRLTAVDNLSFQVQRGNVYGLLGPNGSGKS
                     TTLGMLLTSINPTAGNWQWFGNVKPTDALKRIGATIERPNFYPYLNATQNLLITAKIK
                     GVAVKNIDEKLELVGLLERKKDKFATYSLGMKQRLAIASAMLNDPEVLILDEPTNGLD
                     PMGIIQIREIISTIATTGTTIILASHLLDEVEKVCSHVIVLRNGKSLYSGEVAGMTNS
                     QPYFELAAADMEALRNALSTIREIESVVLSGKIFHVEVREDLDPGTMNKLAFERGITL
                     THLVKKKNSLETQFLELVKK"
     gene            1410..3998
                     /locus_tag="PLG24_10625"
     CDS             1410..3998
                     /locus_tag="PLG24_10625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HMZ25389.1"
                     /translation="MQGQKTPVIDRDLFFGNPEISSGQLSPDGKWITFMKEYEGIMNI
                     WLKKFDEPFDKARPLTDSKRPLYGYYWTDDAKYILYVKDKDGDENINIFAVSPYEKVE
                     NGKLPESRNLTPFKDIAAQIYATSQKNPDVIMIGINNRDKAWHDLYKLTISTGKLDLM
                     YTNTDRITGYDFDWDDNLRVFYRTDEKGNTSFLYKKGDQLTPIYETSVTEQAYISGWN
                     EDNSKFYLITNKGDLNFQTLYMMDPNTQKLTFIESDPNKKVDFGSLKLDRNTRKIIST
                     SYTADKTEYHWKNKAWEANYNFLKSKFPGREVDFQSSTLDYSKFLISVSGDRYVSEAY
                     FFDTKTKNLIFQYTPRPKLKKLEQYLAPMTPVTYPSSDGLMIPAYLTLPTGKTAKNLP
                     TVILVHGGPKGPRDYWGYNSYVQFLANRGYAVLQPNFRASGGYGKQFLNAGDLQWGKL
                     MQDDVTWGVKYLIENGISDKDKIAIMGGSYGGYATLAGLAFTPDVYACGVDIVGPSNL
                     FTLLESVPAYWESGRAFLYGMTGDPNTEAGKQRIRESSPLFHANNIVKPLLIIQGAND
                     PRVKKAEADQIVIALRDKGKQVEYLLADDEGHGFAKPVNNMAMFAAIEKFLANILGGN
                     YQSSMPEDVAKRLKELTVDINSVTYAPKEEVASAASLPKINNTLKEGTSQYDVNFEVQ
                     GQKIPMQMTRVISRENDLFVVTDEATSAMGNMKDQIKYNADFQPVSRSISQMGQTITM
                     TYTPGKIDVDMSGNKMSLDVKGALLNDGPGMDILVAGLPLKEGYTLVAEMPDITTMKT
                     KQVQLSVTGSEVINGVNHLKVEIASTENTADKTTLWINPATGDASKMTQVIPAMGNAV
                     ITTTRK"
BASE COUNT         1264 a          881 c          795 g         1097 t
ORIGIN      
        1 caaaaggata aatattctgg ccaatttata attatagatt ttcagccact ccaattgcaa
       61 taattttttc atatatataa taatttaata atatgatttt agttgtaata caataagcct
      121 tcaactatcc atgaagtttc actttttgac caattccaaa aattgggttt ccagactgtt
      181 ctttttcttc accaggtgag tcagtgtaat gcctctttcg aaagcaagtt tattcattgt
      241 gcccggatcc aaatcttctc taacttctac atgaaatatt tttccactca agactacaga
      301 ttctatttcc cgaattgtcg aaagtgcatt acgtaaggct tccatatcag ctgctgccaa
      361 ctcgaagtaa ggttggctat tggtcatgcc agcgacttca cctgaataca aagattttcc
      421 atttcgcaaa acgatgacat gagagcacac cttttccacc tcatccagca aatggctcgc
      481 caggatgatg gtcgtacctg tagtcgcaat tgtagaaata atctctctaa tctgaatgat
      541 tcccatggga tccagaccat tggtcggttc atccagaata aggacttcgg gatcgttaag
      601 catggcactc gctatagcca accgttgttt catcccaagt gaataggtgg caaatttgtc
      661 cttcttcctt tctaaaagtc cgaccagttc aagtttttca tcaatattct ttaccgccac
      721 gcccttaatc ttagccgtta tgagcagatt ctgagtggca ttgaggtagg gatagaaatt
      781 gggacgctca atggtcgcac caatccgctt cagggcatct gtaggtttga cattaccaaa
      841 ccactgccag ttgccggctg tcggattgat cgaggtcagc aacataccaa gtgtagtgga
      901 tttaccactg ccattgggac caagaagacc atacacattg cctcgctgca cctgaaagga
      961 aaggttgtcg actgctgtta gcctgccaaa cctcttagta agattattta tctcaaggac
     1021 attctccata gaaattcaaa tttgaaaaac gaagatacta ctttattctc cgggcaactg
     1081 tttataccta aattcaactt tgttattaat atatataaat aattattaat ttttataact
     1141 ttaaatttct attttttaca cattataaat tactactata ttattgacac aattccttta
     1201 ctatcgataa ttttgctata agggcatacc aataaattca cttaataccg aataatattt
     1261 atttggtcga ataaattgaa aattactcct caaaatttca atttttacaa aaaattaagg
     1321 gacttttatt ccctcttaaa ttatttaatc caaattatat gaaaaatctt tttttaatct
     1381 cacttgtttt gttacttcaa actaatttta tgcaaggaca aaaaacgcct gtaatcgaca
     1441 gggatttatt ttttggtaac cccgagattt cttcaggtca attaagcccc gacggcaaat
     1501 ggataacctt tatgaaggaa tacgaaggta tcatgaatat ctggctcaaa aaatttgacg
     1561 aaccctttga caaggcgaga ccgctgactg atagtaagag gccgctgtat ggctattact
     1621 ggactgatga tgcaaagtat attctttatg taaaggacaa ggacggtgat gaaaacatca
     1681 atatctttgc cgtctctccg tatgaaaagg tagaaaacgg aaagctaccc gaatcccgca
     1741 acctgacacc ttttaaagat atcgctgccc agatttacgc cacaagccaa aaaaatccgg
     1801 atgtaatcat gataggtatc aataatcggg ataaagcatg gcacgacctg tataaactga
     1861 ctatttccac aggtaaactg gacctgatgt acaccaatac cgacagaatt acgggatatg
     1921 attttgactg ggacgacaat ctccgagttt tctaccgcac tgatgaaaaa gggaacacca
     1981 gttttttata taaaaaaggc gatcaactca cacccatcta tgaaacttcc gtaaccgagc
     2041 aggcttacat ttcaggttgg aacgaagaca attccaaatt ttatctcatc accaataaag
     2101 gcgacctgaa cttccagacc ttgtacatga tggacccaaa tactcaaaaa ctgacgttta
     2161 ttgaaagcga tccaaataag aaagtagatt tcggatcctt aaagcttgac aggaatacca
     2221 gaaaaatcat ttccacgtcc tacacagctg ataagacgga atatcactgg aaaaacaagg
     2281 cctgggaagc caactacaat tttctcaaaa gtaagtttcc cggcagagaa gttgattttc
     2341 aaagttccac actggattat agcaaatttt taatctcagt gagcggtgac cgatatgtgt
     2401 ctgaagccta tttttttgat acaaagacca aaaacctgat cttccagtac acgccacgcc
     2461 caaaactaaa aaaactggaa caatatctgg cacctatgac gcctgtcact tatccgagta
     2521 gtgacgggtt gatgataccg gcgtatctga ctttaccgac cggaaaaacg gccaaaaatc
     2581 ttccgacagt catcttagtt cacggtggtc caaagggtcc gcgtgattac tggggatata
     2641 actcttatgt acagtttctg gccaacagag gttatgccgt ccttcaaccc aatttccgtg
     2701 caagcggcgg ctacggaaaa caatttctca atgccggaga ccttcagtgg ggtaaactca
     2761 tgcaggatga tgtcacctgg ggtgtaaaat atctgatcga aaatggcatc tccgataaag
     2821 ataaaattgc cattatgggt ggaagttacg gtgggtacgc tacattggca ggtcttgcct
     2881 ttacacccga tgtgtatgcc tgtggggtag acatcgtcgg cccaagcaat ttgtttacct
     2941 tactggaatc tgtacctgcc tattgggaat ccggacgcgc attcctgtac ggcatgacgg
     3001 gcgacccgaa tacggaagca ggaaaacaac gcatccggga atccagcccc ttgttccatg
     3061 ccaataatat cgtaaagccg cttcttatca ttcagggtgc caatgacccg agggtaaaga
     3121 aggctgaagc tgatcagatc gtcatcgcac tgagagacaa aggcaaacag gtagaatatc
     3181 ttctcgccga cgatgaagga cacggttttg ccaagccggt caacaacatg gcgatgtttg
     3241 cagctataga gaagttcctc gccaatatcc tcggcggcaa ttatcaatca tctatgccgg
     3301 aagacgttgc taagcgacta aaagaactta cggtggacat aaattcagtt acttatgctc
     3361 caaaggaaga agtggcatca gctgcctcac tccccaaaat aaacaatact cttaaggaag
     3421 gaacttcaca gtatgacgtc aattttgagg ttcagggtca gaaaatacct atgcagatga
     3481 cgagagtcat ttcaagggaa aatgacctct ttgtcgtcac ggatgaagcg acaagcgcaa
     3541 tgggcaatat gaaggatcag atcaaataca atgctgattt tcaacccgta tccagaagca
     3601 tctcacaaat gggtcagaca attactatga cttatactcc gggtaaaata gacgttgaca
     3661 tgagtggcaa taaaatgtct cttgatgtga aaggcgcact gctcaacgac ggtccgggaa
     3721 tggatattct ggtagccggt cttccgctga aagaaggtta taccctagtg gccgaaatgc
     3781 ccgacatcac taccatgaag accaaacagg ttcaattgtc agtgacaggc tctgaggtca
     3841 tcaatggtgt caatcacctc aaagtcgaaa tagcctctac cgaaaacacg gcagataaaa
     3901 caactctctg gatcaatccg gcaacggggg atgcaagtaa gatgacgcag gttattcctg
     3961 ccatgggcaa tgctgtcata acgaccacgc gcaaataaat cgcattaaaa aaggacagac
     4021 cgttaaatct gtccttt
//
LOCUS       DAOQQN010000305         4030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_10648_length_4030_cov_5.564277, whole genome shotgun sequence.
ACCESSION   DAOQQN010000305 DAOQQN010000000
VERSION     DAOQQN010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10648_length_4030_cov_5.564277"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PLG24_10630"
     CDS             complement(<1..828)
                     /locus_tag="PLG24_10630"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HMZ25390.1"
                     /translation="MAQQDDFKRIVSFAKEYGFIYPSSEIYDGLSAVYDYGPLGVELK
                     NNIKQYWWQAMTRLHENIVGIDSGIFMHPLIWKASGHVDAFNDPMLDNKDSKKRFRAD
                     VLIENYIDKVADKINKEVEKAAKRFGDSFDADMFRSTNPRVLEYQKEIDTVSRRLADA
                     MTAGDMAELGKIIADLEIADPDSGSRNWTEVRQFNLMFSTQLGNISGQEDKLYLRPET
                     AQGIFVNFLNVQKTTRQKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVRPG
                     "
     gene            982..1410
                     /locus_tag="PLG24_10635"
     CDS             982..1410
                     /locus_tag="PLG24_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HMZ25391.1"
                     /translation="MDRREFIEKAGLACLAGIAGTSIITATGCKAATPTLTNSTHEGR
                     RVKILQADWPANTDFIIVRHPSDEHPIYIHKDGDNYIALHMKCTHRGCNLNAESNQLT
                     CPCHGSKFHADGKVKKGPAKKPLVGYKTTADTETIYVEIM"
     gene            1536..3329
                     /locus_tag="PLG24_10640"
     CDS             1536..3329
                     /locus_tag="PLG24_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ25392.1"
                     /translation="MFSSLKSLGALNRFLVRYKYYLIGGFIFIALSNYFRVLQPRYFR
                     EALDMIIEVVPMYDVVKGSKHAALLYEYIIENLIWFGIFVILQAVIMGIFMYFMRQTI
                     IVMSRLIEYDMRKELYAHAQYLDTTFYKQHAIGDLMARFTEDVSRVRQYLGPVLLYGF
                     NLITLFVLVITAMFKVNTQLSIFTLLPLPLLSWSIFKISEKINVQSGYIQTQLSKLST
                     LSLEILSGIRDVKGYAREKAMQEMFAEETNVYVDKNLQRAKVDSLFYPLMILVIGISS
                     VLTLWIGGHQVVRGEVTPGNIAEFFIYITLLTWPITALGWTASQVQQAAASQKRINEF
                     LDADVSFKNGTDQIERINQDIRFEHVSLTYPDTGITAVNDISFIIKKGSKTAIIGETA
                     SGKSTIAYLLMQMYKPTEGRILIGDRDISEFSIESWRERIGYVPQDSFLFSDSIRNNI
                     GFGLKDESGFDDVERYAVHASVHEDIMTLPQEYETIVGERGVTLSGGQKQRISIARSL
                     ISNPELLILDNSLSAVDTNTELKIASYLNDVDNSITMIIITQRINNIFNYDRIITMAE
                     GKVIEDGTHDELLKAKGYYYSLYLKLNEGKD"
     gene            complement(3546..>4030)
                     /locus_tag="PLG24_10645"
     CDS             complement(3546..>4030)
                     /locus_tag="PLG24_10645"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HMZ25393.1"
                     /translation="YTTGKGVNGFTLDPTIGEFCLSHRDIKIPENGAFYSVNQGYYLK
                     FDLEMRRYIDYCSDNNLGLRYIGSMVSDVHRILFQGGIFLYPNTRKYPKGKLRMQYEC
                     NPMAFIVEQCGGKAMNTSLQRIMELDAHELHQRSTIVLGSPLMVDEMKRFVEKYSPMI
                     "
BASE COUNT         1220 a          885 c          832 g         1093 t
ORIGIN      
        1 accgggacga acaaaaaact gcatttccat ttgttcgaat tcgcgcatac ggaagataaa
       61 ctgacgcgct acaatctcat tcctgaaggc cttgcctatc tgagcaatac caaaaggaat
      121 cttctgtctg gttgttttct gcacattgag aaagttgacg aagattccct gagcagtctc
      181 aggacgcaga tacaacttgt cttcctgacc ggaaatattt cccagctggg tggagaacat
      241 caggttaaac tgacgcacct cagtccaatt gcgggaaccg gagtccggat cagcgatttc
      301 aaggtcggct ataatcttgc ccaattcagc catatcgccg gcagtcatgg catcggccag
      361 gcgcctggat actgtatcta tttctttctg atattcaagg acccgtggat tggttgacct
      421 gaacatatcg gcgtcaaacg agtctccaaa tcgctttgct gctttctcaa cctctttgtt
      481 gatcttgtca gccaccttgt cgatataatt ttcaatcagc acatcagcgc ggaaccgctt
      541 tttactatcc ttgttgtcca gcatcggatc attgaaggcg tcaacatggc cacttgcctt
      601 ccagatcagt ggatgcatga agataccact gtcgatacca acaatattct catgcaatcg
      661 ggtcatggcc tgccaccagt attgcttgat gttgtttttc aattccacac ctaaaggacc
      721 gtaatcatac actgcactta gtccatcata aatctcactt gaagggtaaa taaacccgta
      781 ttccttagca aatgaaacga ttctcttgaa atcatcctgt tgcgccatat ctgaagaaat
      841 ttaatattat ttggtttcag gccgcaaagg taaatattat tttccacaat gaagaaagcc
      901 aattaatctt ggtataacat cagtctgaac cttcatttga aaaaattttc ctatatttag
      961 tccacgtaaa actataggaa catggataga agagaattca ttgaaaaagc aggtctggct
     1021 tgtctcgcag ggatagccgg gacgagtatt atcactgcca caggctgtaa agctgctact
     1081 cctacattga ccaacagcac acatgaaggc agaagggtaa aaatcttaca ggctgactgg
     1141 ccggcaaata ccgattttat catagtacgc catccgagcg atgagcatcc catctatatc
     1201 cacaaagatg gagataacta catcgcactg catatgaaat gtactcaccg cggatgcaat
     1261 ctcaatgctg aatccaatca attgacctgt ccttgtcacg gaagcaaatt ccatgctgat
     1321 ggaaaggtca aaaaaggtcc cgccaaaaaa ccattggtcg gctacaaaac cacggctgat
     1381 acagagacca tctatgtaga aataatgtag actgtcagca catcctcccg ggaggagtaa
     1441 gaaaacaagc tatcttctac cctggaatga cgttttgtcc catcaatcat aagacaaaaa
     1501 cgctacattc ccttgaatca tattatcttt ggtcgatgtt ttcttccctt aaaagtcttg
     1561 gcgctctcaa tagattcctt gtccggtaca agtattacct gatcggtgga tttattttta
     1621 ttgccttgtc caattatttc agggtattac aacccaggta ttttcgggag gcgttggata
     1681 tgatcattga ggtggtgccc atgtacgatg ttgtcaaggg cagcaagcac gccgcattgc
     1741 tctatgagta tattatagaa aatctgatct ggtttggcat attcgtaatc ctacaggctg
     1801 tcatcatggg gattttcatg tatttcatgc gtcagacaat cattgtcatg tccaggctta
     1861 ttgaatatga tatgcgcaag gaattgtatg ctcatgcgca atatttagac acaacttttt
     1921 ataaacagca tgctattggt gatctaatgg cacgatttac cgaggatgta agtcgtgtcc
     1981 gtcaatatct gggaccggta ttgctttatg gcttcaacct gataacctta tttgtattag
     2041 tcattacggc gatgtttaag gtcaatactc aattatctat ttttaccttg cttcctttgc
     2101 ccttgcttag ctggagcatc tttaaaataa gtgagaaaat caatgtgcaa agtggttata
     2161 tacagaccca actgtcaaag ttgagtactt tatcgctcga aatcctaagt ggcataagag
     2221 atgtcaaggg atatgcccgg gaaaaagcga tgcaagaaat gtttgccgaa gaaaccaatg
     2281 tttatgttga caaaaactta caaagagcca aggtagattc tctattttat ccgttaatga
     2341 tccttgtgat cggtatttct tccgtgttaa ctttgtggat aggtggacat caggtggtgc
     2401 gcggcgaagt aacgcccgga aatatagcgg agttcttcat atacatcact ttattgacat
     2461 ggccgatcac cgcattaggg tggacagcat cacaagtgca acaagcagca gcttcccaaa
     2521 agagaatcaa cgaattttta gatgctgatg taagttttaa aaacggaacg gatcaaatag
     2581 aaagaatcaa ccaggatatt cgctttgagc atgtgtcttt aacttatcct gatacgggaa
     2641 taacggcggt caatgatata tcatttatta ttaaaaaagg cagcaaaaca gccatcatag
     2701 gcgaaacagc ttccggcaag agcacgattg cctacctatt gatgcagatg tacaagccga
     2761 cagaaggtcg catcctgatt ggggacagag atatctccga attttccatt gaatcatggc
     2821 gggaaagaat cggctatgta ccacaggatt cattcctgtt ctcggactcc atccgaaaca
     2881 acatcggctt tggcctaaag gacgaatcgg gattcgatga cgtggagaga tacgccgtac
     2941 atgcttctgt ccatgaagat atcatgacct taccgcagga atatgagacg atagtaggcg
     3001 aaagaggcgt aaccttatcc ggtggccaga aacaacggat ttccatcgcc agatcgctga
     3061 tatccaatcc ggaactactg attctggaca atagcctttc cgctgtggat acaaatacag
     3121 aattgaaaat cgcctcttat cttaatgatg ttgacaacag catcaccatg atcataataa
     3181 cccagcggat taataatatt tttaattatg acaggatcat cacaatggca gaaggaaagg
     3241 tcatcgagga tggcacacac gacgaactac tcaaagccaa gggatactat tactcattgt
     3301 acttaaaact aaatgaaggt aaggattgag aagagtctta tccggaactc ctgttattca
     3361 gtataaacga acactaccac ccaatacata gtcaatcggg caatcggaac gcaattagtc
     3421 aggaacaacc taactgacca tttgatctat ttggttaacc ggctatcatg caggtacaaa
     3481 aatcaacaag cgggaacaag gagactatac gcaattctta aaatgaatac cggcatttac
     3541 cgggattaaa tcatcgggct gtatttctct acaaaccgct tcatctcatc caccatcaat
     3601 ggagacccta aaacaatggt agatcgttgg tgcaattcat gagcatccaa ctccataatc
     3661 cgctgcaagg aagtattcat cgctttgccc ccacactgtt caacaataaa agccatcggg
     3721 ttacattcat actgcatcct caacttgcct ttagggtatt tgcgtgtatt gggataaagg
     3781 aagatgccac cctggaacaa aatccggtgt acatcagaga ccatagaacc gatataacgc
     3841 aagccaaggt tattgtcaga acaatagtca atatagcggc gcatctccag atcaaacttc
     3901 agataatatc cttgattgac tgaataaaaa gcaccatttt caggaatctt aatgtcccgg
     3961 tgggaaaggc agaattcgcc gatggtcgga tcgagtgtaa aaccattgac tcctttgcct
     4021 gtggtatata
//
LOCUS       DAOQQN010000306         3945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_11043_length_3945_cov_5.150386, whole genome shotgun sequence.
ACCESSION   DAOQQN010000306 DAOQQN010000000
VERSION     DAOQQN010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11043_length_3945_cov_5.150386"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..2308)
                     /locus_tag="PLG24_10650"
     CDS             complement(209..2308)
                     /locus_tag="PLG24_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HMZ25394.1"
                     /translation="MIEMIYQATGLREQQVRAVLELIQEGATIPFIARYRKDRTGGLD
                     EVKIAGIITLHRQIIALEERRTFILESLAEKGIGDEKLLKMIREARDLQTLEDLYSPY
                     KSRRKTRADMARERGLEPLAKSIMSQRNMDVEAIAFKYTCKEVPEVADALQGARDIIA
                     EWIADDVTLKASLRKLFASKATVGSKVVKAKAEEAGKYRDYFDFKENIRKIAGHRFLA
                     ISRGADEGFLRLSIEPEIRDALDSIDRRYIRARNSAASQVEKAIDDAYKRLIQPSLET
                     EFRNELKEKADKEAIDVFAQNLRQLLLAGPAGEKCTMAIDPGYRTGCKIAILDVNGNL
                     IATDLIYIHEPNRLAASRNTIESLIKKHSVEIIAIGDGTAGRETERFVKNIPVTLPVF
                     LVNEDGASIYSASGLAREEFPDLDLTYRGSISIGRRLMDPLAELVKIDPKNVGVGQYQ
                     HDVNQALLKETLDRTVESCVNAVGVNLNTAGKELLAYVSGIGPVLAKNIVSYRTQHGA
                     FRSRKELLEVPRLGPKVYQQAAGFLRIKDGENILDDSGVHPESYDIVKQISKDLKMPI
                     EVLIGNPILDNLDTSRYQDENRGELTLKDIIAELKKPGLDPRTEASAFEFASVYSIDE
                     LYEGMILPGKVTNLTKFGAFVDIGVKQDGMVHISEIANKYIKDPADELKLNQHVMVRV
                     LQVDKERQRISLSIRQV"
     gene            complement(2406..3440)
                     /locus_tag="PLG24_10655"
     CDS             complement(2406..3440)
                     /locus_tag="PLG24_10655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMZ25395.1"
                     /translation="MRYIFFVLTLFSIGNLQAQERYLTQFYGSPITLDPSLTGNFEGN
                     YRVNLAYRNQWSRNFEKPFTAFLGSVDLNFDLGLKSQKVHDIVSAGLYFAHDKAGVLD
                     LGNTEMGIAGSYHKALGPNQFLSGGLYLSLNQRNSNFTNVTFEDQFNGEDGYTQPTAE
                     SLAANNFSYFDQGVGVNYSNYPEKQNGFNVGISVAHLSRPEASFYKRDLNSEESAISF
                     RLPMKYTLHAGGRLVLDDYMSLYPRLMYQRQQPFDMATVGLTLATDVYFWTIHTGVFA
                     RGAGVNNKYNFDSAGFLLGIEYNKWQVGVSYDIGFPSFIYYNKNQGTIEITVSYFGLY
                     VNDDLLCPTF"
     gene            complement(3688..>3945)
                     /locus_tag="PLG24_10660"
     CDS             complement(3688..>3945)
                     /locus_tag="PLG24_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25396.1"
                     /translation="NNLSVRTGLVSSILFNLVVIASYRQNKVSTDKLKVFIGFFLEGA
                     GDFLLKKQIPGKFILRVTPDEINSPRCWRKAKSYTGQNLSV"
BASE COUNT          975 a          919 c          897 g         1154 t
ORIGIN      
        1 aggatgcttg tcggcttacc gaagtaggta ggaaaataat acacaggacc ctttacctga
       61 ttgagccggc ataattcggc aaattttctg accccaaaaa agaggatttt tttaaattga
      121 tgttggccaa tcacctctgc tgttggctcg ataagtatta gtattctacc taattacttt
      181 tgcccggtag ctaatcgagc cttcatccct acacctgcct gatcgacagg ctaatccgct
      241 gccgctcctt atctacctgc aacacccgga ccattacatg ttggttgagc tttagctcat
      301 ctgcgggatc cttgatgtac ttgttggcga tttcagagat atgcaccatg ccgtcctgtt
      361 ttactcctat gtcgacaaaa gctccgaatt tggtcaggtt ggtcaccttc cccggcagga
      421 tcatgccttc gtataattca tcgatgctgt acacagaggc aaattcaaag gcagaagcct
      481 ccgtccgtgg gtccagtccc ggctttttta gttcggcaat gatatccttc agcgtcaatt
      541 caccgcggtt ttcatcctga taacgactag tgtcgaggtt gtcaagaatg ggattgccga
      601 tcaaaacctc gatgggcatt ttgaggtctt tggatatctg ctttacaata tcataggatt
      661 ccgggtggac gccactgtcg tccagaatat tttctccgtc cttaatacgc aggaaacccg
      721 ctgcctgttg atacactttt ggtcctaagc gtggcacttc gagcaattcc tttctggacc
      781 ggaatgcccc gtgctgtgtg cggtaagata cgatattttt agccaggacg ggcccaatac
      841 cacttacata ggccagaagt tccttgcctg cagtattgag attgacaccg accgcattga
      901 cacaggactc tacggtacgg tccagcgttt cctttagcaa ggcttgattt acatcgtgtt
      961 ggtattgtcc cactcctacg tttttagggt ctatcttgac gagttctgcc agagggtcca
     1021 tcagacgacg gcctatggat atggagcccc gataggttag atcgagatcc ggaaattcct
     1081 ctcgtgccaa cccggacgcc gagtagatgg aagccccgtc ttcattgacc agaaataccg
     1141 gcagcgttac cgggatgttt ttgacaaacc gctctgtctc ccgtcctgcc gttccatccc
     1201 cgatagctat tatttcaaca ctatgttttt taatgagtga ttctatagta ttgcgggatg
     1261 ctgccagccg gttcggttca tggatataga tgaggtcggt agctatcagg ttgccattga
     1321 catcgagaat ggcgatttta catccggtgc gatagcccgg atcgatggcc attgtacatt
     1381 tttctcctgc cgggcctgcg aggaggagct ggcgaagatt ctgggcaaat acgtcaatgg
     1441 cttccttatc cgccttttct ttcagttcat tcctgaattc ggtttccagg gatggttgaa
     1501 tcaatcgctt gtaagcatca tcgatggctt tttctacctg ggaggcagca ctgttacggg
     1561 ctcgaatata acgtcggtcg atgctatcaa gtgcatcgcg tatttcgggt tcaatactaa
     1621 gcctcaggaa gccttcatca gcacctcggc tgatggccag gaaacggtgt cctgctatct
     1681 tcctgatgtt ttctttaaag tcaaaataat cccggtattt gccggcttct tcagcttttg
     1741 ccttgacaac cttactacct actgtggcct ttgacgcaaa gagttttcga aggctggctt
     1801 taagcgtcac atcatcagcg atccattccg cgatgatgtc gcgtgcaccc tggagggcat
     1861 ctgctacttc cggtacttct ttgcaggtat atttgaaggc gattgcctcc acgtccatat
     1921 tgcgttgact catgatggat ttggccagtg gttcaagtcc tctctctctc gccatatcgg
     1981 ccctggtctt tcttcttgat ttgtagggag agtacagatc ttccagcgtc tgaagatcgc
     2041 gagcctcccg gatcatcttc aataattttt cgtccccgat gcctttttcg gccaggcttt
     2101 cgaggatgaa tgtccgacgt tcttccaggg cgattatttg tctgtgcagg gttatgatcc
     2161 cggcgatctt tacctcatcc agtccacctg tcctgtcctt acggtatctg gctataaaag
     2221 gaatcgtagc accttcctgt atgagctcaa ggacggctct tacctgttgt tcccgtaggc
     2281 ctgttgcctg gtatatcatt tcaatcatta tacagcttgt ttttggagat actgctgaca
     2341 tagtgcagta catgggtgta aatattcatc cttcaggagt cactcagatg cctaccggaa
     2401 aggtatcaga aagtagggca caacaagtcg tcattgacat agaggccgaa ataggaaacc
     2461 gttatctcaa ttgttccctg atttttattg taatagataa aactcggaaa acctatgtca
     2521 taactgacgc ctacctgcca tttattatat tctattccca ataaaaatcc ggcagaatca
     2581 aaattatatt tgttgttgac acctgcgcct ctggcaaaaa cccctgtatg tatggtccag
     2641 aaatacacat cagtcgccag tgtcaatccc acagtagcca tatcgaaggg ttgttggcgc
     2701 tgatacatca aacgggggta gagagacata tagtcatcaa ggacgaggcg accacctgcg
     2761 tgaagggtat atttcattgg cagcctgaag gagatcgcac tttcttctga gttgagatcg
     2821 cgcttataga aagatgcttc agggcgggaa aggtgagcta ccgatatacc tacattgaaa
     2881 ccattttgct tctccggata gtttgaataa tttaccccta caccctgatc gaagtaggag
     2941 aagttgtttg cagccaggct ttccgcggta ggttgtgtgt aaccgtcttc gccattgaac
     3001 tgatcttcga aggtgacatt ggtaaagttg ctgttccttt gattgaggct gaggtaaagg
     3061 ccacctgaca ggaactgatt gggacccaat gctttgtggt aggatccggc aatacccatt
     3121 tctgtgttac ccagatcgag tacaccggct ttgtcatggg caaaatacaa tccggcagat
     3181 acaatgtcat gaaccttttg gcttttcaag ccgagatcaa aatttaagtc cacgctgcct
     3241 aagaaggcgg taaatggctt ttcgaagttg cgcgaccatt gattcctgta agcgagattg
     3301 acccgataat tgccttcgaa attacccgtc agtgatggat caagagtaat tggcgagcca
     3361 tagaactgtg tcagatagcg ctcctgcgct tgcaggttgc cgatggaaaa taatgtcaat
     3421 acgaaaaaaa tatacctcac caaatgtgaa tttatttgat taaatcaggt gcaaagctac
     3481 acacataccc ttagtaaacg aaagaaatgc tattaaatta ttgcaaataa gcactacttt
     3541 gtatgggtaa gggatgaata tccttccaac aaaatattcg tccatggaaa tattggattg
     3601 gaaaatcatt gaccgagatt attgaccctg ttattccttg tgattctgtt ataatatctc
     3661 aacttcagat tttgtgagag gaatgactta cacggacagg ttttgtcctg tgtaggattt
     3721 agctttgcgc cagcatcggg gagagttgat ttcatcaggt gttaccctta atataaattt
     3781 gccggggatt tgcttcttta gtagaaaatc tccggcccct tcaagaaaaa atccaataaa
     3841 taccttaagt ttatccgtac taaccttatt ttgtcgatat gaggcaatca cgactaaatt
     3901 gaacaaaatg gatgatacca gacctgtccg gacactcaga ttatt
//
LOCUS       DAOQQN010000307         3919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_11148_length_3919_cov_5.620342, whole genome shotgun sequence.
ACCESSION   DAOQQN010000307 DAOQQN010000000
VERSION     DAOQQN010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11148_length_3919_cov_5.620342"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2754
                     /locus_tag="PLG24_10665"
     CDS             <1..2754
                     /locus_tag="PLG24_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25397.1"
                     /translation="IVATTTDTTYRLAGLNPDIDEMISVAAVLPDGGVSRRDHTQFVK
                     TLGVQCDHKFDLEILEITSPERFGRELTSTALTNEEKVSIRIVNKGLEAAQGFSIHYV
                     IDNIETVEVFNELILPGETAEYTFQQPARLSEAGNHQLRVWIDYNFDSPYHSDNAKEI
                     LIRQIPNKEIVLSRELSSTFHLGFDDQHTGVARNGDKGILGVDEIDFHSNNPRGRARM
                     VVPSGFNKSGKGALTMDVDYQGTSVQNEAIFTFNLSKYTLDDDINLELSYLNQNSPLK
                     VDSLTAKLWIRGSDSDIWVEAYNLKENEARAGTYTSIRSLRLDKLLRDANQQFSTSTQ
                     LKISQLVDGPAVARDGFSGYTFDDLYLYQSQRDMQVTRIISPAPNSCGLTADEEITLE
                     IANTSNEPVDSLSLWYSVDGVVTGPIDIPPMLGTEVRTISGLPADLSTIGAHTIKVWV
                     AAPGDTYRANDTLSGYTVYNNGFVGQFPYLATFEAGNETWISSGFQSSWIRATPDKDN
                     LQGAANGDFAMVTSSDGRYNGYERSTLTSPCFDLSGLQEPYLSFSFWHDTEVEYDSCW
                     VEYSVDGSAWKILENTHRSINWYNRPFAWDGQDDHWHVAGLPLPVQLFTDRSGIQFRF
                     VLYADENVQKGGMAIDDIHIYEGPDIYVGGDNKVSFLANQHWQDIVVDGRTIAAIKSE
                     DPQPEPFTVGVHFNTGTEQRHDQSQYYLDRSLSVNTASGTAKPYNIRFYFTDEEAERI
                     IHAGPGYHAPNSAYQLGLTSISSEDNDGVFGNEVNPEYDFFSFKRTQLVPYKDGYYLE
                     FRAKGPGEFFLNSGGKEFNLPLGNTTSNVFGVGLAGDNLTARPNPFSDEFVIYLETAQ
                     KSKIYRVELVDVSSRIVNTYMVPGESLSEGFPVRTRLPAGVYLIRIHGSDLHAVSRLV
                     RVD"
     gene            2939..>3919
                     /gene="mnmA"
                     /locus_tag="PLG24_10670"
     CDS             2939..>3919
                     /gene="mnmA"
                     /locus_tag="PLG24_10670"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HMZ25398.1"
                     /translation="MKRVVVGLSGGVDSSVAAYLLKEQGYEVIGMFMRNWHNDEVILS
                     NECCWVEDSNDALLVADKLGIPYYVIDLSAEYKTRIVDYMFAEYEKGRTPNPDVLCNR
                     EIKFDVFLEAAMKLDADFVATGHYCRKTTDANGRHHLLAGKDGNKDQSYFLCQLNQDQ
                     LAKALFPIGDLLKSEVREIARREGLVTAEKKDSQGLCFIGKVDLPTFLQQQLQPRKGD
                     IIEIPQELERLVQYHEIPVSPENIEALSSPFTFEATEGIKVGEHRGAHYYTIGQRKGL
                     HIGGRPYPSFVLQTDTRHNIVFSGQQSDHPGLNRYALKIEADEIHWVNPDY"
BASE COUNT         1047 a          881 c          986 g         1005 t
ORIGIN      
        1 attgtcgcca ctactaccga cacaacatac aggcttgccg gtcttaatcc ggacatcgat
       61 gagatgatca gtgtcgcagc tgtgttgccg gatggcggcg ttagtcgacg tgatcatacc
      121 cagtttgtca aaactcttgg tgttcaatgt gatcacaaat ttgatcttga aattcttgaa
      181 attacatcac cggagagatt cggcagggag ttgacatcca cagcattgac caatgaggag
      241 aaggtgtcca taaggatagt taacaaaggc ctggaagcgg ctcagggatt cagtatacac
      301 tatgtcatcg acaacatcga aacggtggaa gtattcaatg aattgattct ccccggagaa
      361 acagccgaat atacctttca acaacctgcg agattatctg aagccggaaa tcatcaactc
      421 cgggtgtgga tagactacaa ctttgattca ccctatcatt cggacaatgc aaaagagata
      481 ctcatcaggc agattccgaa caaggagatt gttttaagcc gggagctaag ttcaactttt
      541 caccttggat tcgatgacca gcataccggt gtggctcgca atggggacaa aggcatcctc
      601 ggcgtggatg aaattgattt tcattccaac aatccacgcg gaagggcccg aatggtagtg
      661 ccgtcagggt tcaacaagtc gggaaaaggt gccctgacta tggatgtcga ttatcaggga
      721 acatccgtgc agaatgaagc aatatttacc ttcaacctaa gcaaatatac cttggacgat
      781 gatatcaatc ttgaattatc ctatctgaat caaaattcac cgctgaaagt agatagtttg
      841 acggccaagt tgtggattcg gggaagtgac agtgatatct gggttgaagc atataaccta
      901 aaggaaaatg aggcccgggc cggaacctat acttcaatcc gaagtttgag attggataaa
      961 ttgctgcggg atgccaacca gcagttttcc acaagtacac agttgaaaat aagccagctt
     1021 gtggatggac ctgctgtggc aagagatggt ttttcggggt atactttcga tgatctgtat
     1081 ctctatcagt ctcaaaggga tatgcaggtg accagaatca tcagtcccgc acccaattcc
     1141 tgtggcctca ctgccgatga agaaataacc ctggaaatag caaacacttc gaatgagccg
     1201 gtggattcct tatcgctatg gtacagtgtt gacggtgtgg ttaccggacc gatcgacatt
     1261 ccaccgatgc ttggcaccga ggtgaggaca ataagcggct tgccggcaga tctgtcaaca
     1321 ataggcgctc acaccattaa ggtttgggtt gccgcaccgg gagatactta ccgggctaat
     1381 gatacgcttt cgggatatac cgtttataac aatggcttcg tcgggcaatt cccctatctg
     1441 gctaccttcg aggcaggcaa tgaaacctgg atttcttccg gatttcaatc atcctggata
     1501 cgtgcaacac cggacaaaga caatctgcaa ggcgcagcca atggtgattt tgccatggtt
     1561 acctcgtccg atggcaggta caatggctat gagcgttcta cgcttacatc accgtgtttt
     1621 gacctgtccg gcctgcagga accctatctt tctttttctt tctggcatga tacagaggtg
     1681 gaatatgata gttgctgggt ggaatatagt gtggatggaa gtgcctggaa aatactggaa
     1741 aatacacata gaagtattaa ttggtacaac agaccattcg cctgggatgg tcaggatgat
     1801 cactggcatg tggcgggctt gcctcttccg gtacagctct ttaccgatcg gtccgggatt
     1861 caattcaggt ttgtcctcta tgccgatgaa aatgtacaga aaggaggaat ggccatcgat
     1921 gacattcata tttatgaggg acctgatatc tacgtcggcg gtgataacaa ggtcagtttt
     1981 ctggcaaatc agcactggca agacattgtc gtcgacggca gaaccatcgc cgccatcaag
     2041 tcagaagacc cgcagcctga accttttaca gtgggtgtac atttcaatac cggaaccgaa
     2101 caaaggcatg atcagtcgca atattatctt gacaggagcc tttccgtcaa taccgcctcc
     2161 ggcactgcaa agccttacaa catccgattt tattttactg atgaagaagc tgagcgcatc
     2221 attcatgccg gtccgggcta tcatgcgcct aactccgcat accaactggg cctcacttca
     2281 atatcatcag aggataatga tggggttttt ggcaatgaag tgaatccgga atatgatttc
     2341 tttagtttta aaagaactca actggtgcct tataaggacg gttattacct tgaattcagg
     2401 gcaaaggggc ccggcgaatt tttcctcaat tccgggggca aggaattcaa tcttccgctg
     2461 ggcaacacaa caagcaacgt ctttggtgtt ggtttggccg gggacaattt gactgctcgc
     2521 ccgaatccat tcagtgatga atttgtcatt tatctggaga cagcgcaaaa gagtaaaata
     2581 tatagggtag aattggtgga tgtatcgtcc agaatagtca atacctatat ggtgcccgga
     2641 gagtccttgt cggaagggtt tcctgtcagg actcgtctcc cggcaggcgt gtacctgata
     2701 cggatacacg gaagtgatct ccatgcagtg agtcgcctgg tcagggtgga ctgaaggcag
     2761 gcaattgtat gaccggcccc caatccacaa ctaatagcct gcaggtttgt tatatcggac
     2821 aatcaactgt ggcttgcccc gggcgagggg agatacagtc tgtttataac ggtctgtcat
     2881 cctgttgttt gtaattattc tgatttcttt tcatgttgaa ttgccatatc tttgcatcat
     2941 gaagcgtgtt gtcgtaggat tatcgggagg tgttgactcc agtgtcgctg cctacctgct
     3001 caaggagcag ggatatgagg taataggcat gtttatgcgc aactggcaca atgatgaggt
     3061 catcctttcc aatgaatgtt gttgggtaga ggattcaaac gacgcactgc tggttgcgga
     3121 taagctcgga attccttatt atgtgatcga tctgagtgct gaatataaga cgcgcatcgt
     3181 ggactacatg tttgcggagt atgaaaaagg acgaacgccc aatccggatg tgctgtgcaa
     3241 tcgggagatc aagtttgatg tatttctgga agcagctatg aagctcgacg ccgactttgt
     3301 ggcgaccggt cattattgcc gaaagacaac cgacgctaac ggccgtcatc atttgctggc
     3361 aggaaaagat ggtaataaag accaatccta ttttctgtgt caactcaatc aggaccagct
     3421 cgccaaggca ttattcccta tcggcgactt gctgaagtca gaagtgcgtg aaattgcacg
     3481 acgtgaaggt cttgtaactg ccgagaaaaa ggattcacaa ggcctttgtt tcatcggcaa
     3541 ggtggatctg cctactttcc tccagcaaca actacaaccc aggaagggcg atatcatcga
     3601 gattcctcag gagttggaac gcctggtgca gtaccatgag attccggtat ctccggaaaa
     3661 catagaggcc ctttcttctc catttacctt tgaagcaacg gagggtatca aagtgggcga
     3721 gcaccgcggc gcccattatt acaccatcgg acagcgtaag ggtctgcaca tcggtggccg
     3781 gccgtatcca tccttcgttt tacagactga caccaggcat aatatcgtct tttccggcca
     3841 acaaagtgac catcccggac tcaataggta tgcattgaag attgaggcag atgaaattca
     3901 ttgggtcaac ccggactat
//
LOCUS       DAOQQN010000308         3917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_11160_length_3917_cov_4.531849, whole genome shotgun sequence.
ACCESSION   DAOQQN010000308 DAOQQN010000000
VERSION     DAOQQN010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11160_length_3917_cov_4.531849"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1638)
                     /locus_tag="PLG24_10675"
     CDS             complement(<1..1638)
                     /locus_tag="PLG24_10675"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HMZ25399.1"
                     /translation="MFGPGRNIHGQNLLIDTKVKNEILQNPAKFGLSQNDANQLLYIT
                     SYTDDKRGISYSYVLQTVSGIPVYNAVTSFYVNKKGDVGTVKSNFINSDTYRRDGSRA
                     TAIDVEEAVRSAAVACGIVSPQTRIIETRDNKTILDGGNISSAPIEPFLTWVVRDGAL
                     VLGYQLEIAIIGALDHYQVIVDSRDGTLLNKINYKLSCKFEGPGQYANHSHDSRCLDN
                     HQSNILASTATPAPPPSGEVSKYRVYELPIQAPNFGSSKVVVSPFDTVASPYGWHDLD
                     GKEGMDATNLQGNNVHAFNDNSGNYQPDFEVDGGAELNFDFSFDQNAEPQNSKDAAAV
                     NLFYMINKMHDFSYHYGFDEKAGNFQANNYGKGGEGGDYVKAMSQFGNGEGDFKNNAD
                     FLTPEDGGSGQMRMFLWDNASDKFNIEAPASIKGGYPVGNPQFGSAITTDKIEAEVVW
                     AQDKTGGTNYFCDEAKNADEMSGKIVAIDRGSCDFSLKVYNAQKAGAVGAIIMNFEDR
                     IVDMASGTNGNLVTIPSVFVSKVVGDKIKDELKAGNTV"
     gene            1762..2511
                     /gene="rlmB"
                     /locus_tag="PLG24_10680"
     CDS             1762..2511
                     /gene="rlmB"
                     /locus_tag="PLG24_10680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HMZ25400.1"
                     /translation="MEKQNIPYIYGRQPVIEAIKSGQPIEKIFLLSGGEREFEREIRD
                     GIKGLNIDLAYVPREKLNRLVHGNHQGVVAYINDYSYVTLDKLLHQIKTKNEPAFLMM
                     LDQITDVRNFGAISRSALLGGCHGIIIPSHNSVSVTSDAIKASAGALTRIAVCKVNSL
                     PEAADHLAQQGIQLFASGMGNSVLLHQLPLDEACCIVMGSEKSGVSKALESRADKIFS
                     IPQNELLDSYNVSVATGIVVYQTILKRKYNL"
     gene            2559..3509
                     /locus_tag="PLG24_10685"
     CDS             2559..3509
                     /locus_tag="PLG24_10685"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HMZ25401.1"
                     /translation="MRHINNRKKTSLSNKGRTFLLYATGIFFLITTTVSCSHRSTIKI
                     AQETAPSNALLWKIEGKDLAKPSYLFGTIHLIPQDKFLFNDVMNKAFSECDEIVFEID
                     MNIMNDFSQMFGLLSKIRMPDDMTIKDLLSEDDYKLFQDKFADKGLPAFLLETIKPLF
                     ISTLADGMDSPNQSMISYEIELANKANDQNKTIGGLETIDDQLAAIDSIPLKIQAQML
                     IDELKKEHGENEYERLVALYIQQDIDALQKAINQGMSKDDQTMMNFLLKDRNVKWIPK
                     MNAMMKERPTFFAVGAGHLGGEYGVIKLLKEAGYKVSAIP"
     gene            3743..>3917
                     /locus_tag="PLG24_10690"
     CDS             3743..>3917
                     /locus_tag="PLG24_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HMZ25402.1"
                     /translation="MALILPVNEISPQWGEDCFFAENATITGDVIMGNQCSVWYNTVI
                     RGDVHYIRMGHKVN"
BASE COUNT         1210 a          838 c          735 g         1134 t
ORIGIN      
        1 gactgtattt cctgctttca attcatcctt gatcttatct ccaacaacct tggagacaaa
       61 aactgaaggt atagtaacta agtttccatt ggttccacta gccatgtcca caatacggtc
      121 ctcaaaattc ataataattg cgccaacagc tcccgccttt tgagcattgt acaccttcag
      181 tgagaaatcg catgagcctc tatcgattgc tactatttta cctgacattt catcagcatt
      241 cttcgcttca tcacaaaaat aatttgtccc acctgtctta tcctgtgccc aaaccacttc
      301 agcttcaatc ttatccgttg taatagccga cccaaactga ggatttccca ccgggtatcc
      361 acccttgata cttgcaggtg cttcaatatt aaatttatct gaggcattat cccataaaaa
      421 catcctcatc tggccacttc cgccatcttc cggagtcaaa aaatcagcat tgttcttaaa
      481 atctccttct ccatttccaa actgtgacat agcctttaca taatcacctc cttcacctcc
      541 ttttccataa ttattagcct ggaaatttcc cgctttttcg tcgaaaccat aatggtagga
      601 gaaatcatgc attttattaa tcatataaaa caagttgact gctgcagcat cctttgaatt
      661 ctgaggttct gcattttgat cgaaagagaa gtcgaaattt aattctgccc caccatcaac
      721 ctcgaaatcc ggttgataat taccgctatt gtcattgaat gcatggacat tattcccctg
      781 cagattagtg gcatccatgc cctcttttcc atccagatca tgccatccat atggtgatgc
      841 aaccgtgtca aaagggctta ctacgacctt ggatgaacca aagttgggag cttgaatcgg
      901 aagttcgtaa accctatatt tgctgacttc ccctgaggga ggcggtgcag gagtggcagt
      961 acttgcaagt atatttgact gatgattgtc cagacatcgt gaatcatggg aatgattggc
     1021 atattgtcca gggccctcaa acttgcagga tagcttataa ttaatcttat tcaataatgt
     1081 tccgtcacgg ctatccacga tcacctgata atgatccagt gcacctatta ttgctatttc
     1141 aagctgatat cctaaaacca atgccccatc tctgacaacc caggtcagga atggctctat
     1201 tggagctgat gaaatatttc ctccgtccag aatggtttta ttatctcttg tttcaattat
     1261 ccgtgtttgg ggactgacaa taccacaagc tactgcagcg ctcctgactg cctcttccac
     1321 atcaattgcc gtagccctac ttccatccct tcgataagta tcgctgttga taaagttgct
     1381 ctttacagta cctacatcac cctttttgtt gacataaaaa ctagtaaccg cattatatac
     1441 cgggatacct gataccgtct gcaagacata ggaataggat attcccctct tatcatcggt
     1501 ataactggta atataaagta actggttggc atcattttgg ctcaagccaa actttgcagg
     1561 gttttgtaaa atttcatttt ttacctttgt gtcgatcaaa aggttctggc catgaatatt
     1621 tcggcccggt ccaaacataa agcatgcaaa aagacaaaag gcaattaaat ttcgataaat
     1681 cttaaagtac aacattagat aagttaatta taaattttgc caaagatata atttattcat
     1741 atattcattt tcaaataaaa tatggagaaa caaaatattc cctatatata tggcaggcaa
     1801 ccggtgatcg aagcaattaa gtccggccaa cctatagaaa aaatatttct actatccggt
     1861 ggagaaagag aattcgaacg ggaaatcagg gatggtatca aaggtctcaa tattgatttg
     1921 gcttatgtcc caagggaaaa gctcaacaga ctagtacatg gcaaccatca aggggtcgtt
     1981 gcttatatca atgactattc atatgtaact ctcgataagc tacttcatca gatcaaaact
     2041 aaaaacgaac cggcatttct tatgatgctg gaccaaatta ccgatgtcag aaattttggg
     2101 gcaatatcac ggtctgcctt gttgggcggt tgtcatggta tcatcatacc gtcccataat
     2161 agtgttagtg ttacttctga tgcaatcaaa gcatcagcag gggccctgac acgaattgca
     2221 gtttgcaaag tcaattcctt gccggaggct gctgatcatc ttgctcaaca aggtattcaa
     2281 ctgtttgctt cgggaatggg caattcagtg ttgcttcatc aacttccttt agacgaagca
     2341 tgttgtattg tgatgggttc cgaaaaatcc ggagtgtcca aagcgcttga aagcagagct
     2401 gacaagatat tcagtatacc tcagaacgag ttgctcgact catacaatgt cagtgttgcc
     2461 acaggaatcg tcgtatatca aaccatcctc aagcgcaaat acaaccttta aactaccatt
     2521 cagcaatcca tcatttcttc atttagtaaa accggtacgt gagacatatc aataatcgta
     2581 aaaagacttc cctatccaac aaaggaagaa cctttctgct atatgccaca ggcatttttt
     2641 ttctgataac caccactgtg agttgttccc atagatcaac aataaaaata gcacaagaga
     2701 ccgccccttc caatgcactt ctttggaaaa tagaaggtaa ggacctggct aaaccaagtt
     2761 atcttttcgg cactatacac ttgattcctc aggataagtt tttattcaat gatgtgatga
     2821 acaaggcttt cagtgaatgt gatgaaattg tctttgaaat agacatgaat atcatgaatg
     2881 atttttcaca gatgtttggc ttactttcta agatcagaat gccggatgat atgactatca
     2941 aagatctgct cagtgaggat gattacaaac tttttcagga caaatttgcc gataagggtt
     3001 tgccggcttt tttacttgaa acaataaaac cgctctttat ttcaacttta gcagatggga
     3061 tggacagtcc taaccagtca atgatatctt acgaaataga gcttgccaat aaggccaatg
     3121 atcaaaacaa aacaataggc ggactggaaa cgatcgatga tcaactggcc gcaatcgaca
     3181 gcatacctct gaaaatacag gcacaaatgc tgatagatga attgaaaaag gagcatggcg
     3241 aaaatgaata tgaaagattg gtggccctgt acatccaaca ggatattgat gctttacaga
     3301 aagccatcaa ccaggggatg agtaaggatg atcaaacaat gatgaatttt ttgctcaaag
     3361 acagaaatgt caagtggata cctaagatga acgccatgat gaaagaaaga ccaacattct
     3421 tcgctgtagg cgcaggtcac ctcggcggtg aatatggcgt aataaagctt ctcaaagaag
     3481 ccggatacaa ggtctctgct ataccttgaa ccatcaactg atagaaaagc gctgagaatt
     3541 aatgaaaatg ggaatacttt cagcgtattt acattcttaa ttatataccg aggtcagtta
     3601 ataggcagtt gaagtatcat ggaggcgcct gtttttgaaa aaagcttagc gtaggagata
     3661 cttgcattag acacaaactt taataaagga catatgtctc taatgcccta tatttacaat
     3721 tataatatat aatgaatcta ctatggcttt gatacttccg gtaaatgaaa ttagtccgca
     3781 atggggtgaa gattgcttct ttgcagaaaa tgccacgata acaggtgatg taatcatggg
     3841 caatcaatgc agcgtatggt acaacactgt tatccggggc gatgtccatt acatccgaat
     3901 gggtcacaag gtcaatg
//
LOCUS       DAOQQN010000309         3907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_11207_length_3907_cov_7.211838, whole genome shotgun sequence.
ACCESSION   DAOQQN010000309 DAOQQN010000000
VERSION     DAOQQN010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11207_length_3907_cov_7.211838"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1545)
                     /locus_tag="PLG24_10695"
     CDS             complement(136..1545)
                     /locus_tag="PLG24_10695"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ25403.1"
                     /translation="MKIHFCGADKEVTGSSHVVELDNGFTFMLDCGLYQGHDDDMKKF
                     NETWLRPPSSIDCVVLSHAHIDHTGRLPRLVKDGFTGNIYCTHATRSLSSILLVDSAM
                     IQEREAEYSNKKWLQKGIKYHSRITDPLYVETDVPPVLKLMISYGYDKWFKIHEDVEV
                     KYTDAGHILGSAAVNIRIHEGGKVRTLAFTGDIGRPERPILRDPVPMDPAEIVITEST
                     YGDRLHAKAPQDKEKLERIIRHTCIEKRGKLIIPSFSVGRTQEIIYMMDHMFNEGRMP
                     KVKVYVDSPLAVNATSIYIAHTECYDDEMAKYLLVDDNPFGFNGLDYIKNVEDSKAIN
                     DSNEPCVIISASGMANAGRVQHHIINNCDKPSTTILIVGYCALGTPGRLIRDGADTIT
                     LHGQTKPILADVEIMDSFSAHGDRNEMTAFLHNQLESAQTVFLVHGDTDAMTFFGAHL
                     STQGFKNIIMPNLGETFKV"
     gene            2068..2532
                     /locus_tag="PLG24_10700"
     CDS             2068..2532
                     /locus_tag="PLG24_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25404.1"
                     /translation="MLGRLKKMLGIDGLRVDLITSETYDIEKGYVDGELSLLAKYDVE
                     IEYIEIRIIEIYTRGRFGNKKTDEYQIGTIRTGFGVNLGANRILPVPFKVPVDYLFSS
                     VDKFGMLNIFTRGLAKGAKLLRGVRSKYRMETEVKIDGSNIKVFNSKPLMIV"
     gene            2854..>3907
                     /locus_tag="PLG24_10705"
     CDS             2854..>3907
                     /locus_tag="PLG24_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25405.1"
                     /translation="MTKVPRSKVVRKAPNLYHDNSHDEDNAWNGLPMMAPPTFHLHAK
                     GTYSSLANKDTKPLQLKPASEQKSKKTPDEKIPYKNQGLSAIKDLFEHDAKILAHHNL
                     KLSENHIIKEKNKYQNDRYGYRKEHSNIAKNLEQLMKTQAQIEQYRYPGSNVTFSNAR
                     PPYKDQFLMVLGRATFRINEEGFKKAKAIYPRLQNLYSFIDLLIEKLISNNVFLTGLH
                     ARGKVEEYNQMSGLDQLAAIQEILNDIDFNIKGAREKIISGELSVYDLSPVHQQMASS
                     PKAAGQYRDWRTPGMRDAMQTILQEKKTSDFWKGLGLDALTVMLAVGAGFANPVAAIA
                     MISSNIAISGAKAKSAW"
BASE COUNT         1271 a          783 c          760 g         1093 t
ORIGIN      
        1 taaagacaat gaaaacatga acggcatcat ttcacgccaa attgacactg atctttccaa
       61 attatctcaa taccagagtg cattaatgcc ttgtataagt tggaatttgg ttaaaagtcc
      121 ccaaatttca caccatcaaa ctttgaaggt ctctccgaga ttaggcatga taatgttttt
      181 gaaaccttgt gttgacagat gtgcaccaaa aaaggtcatt gcatccgtat cgccatggac
      241 caaaaaaact gtttgggcgc tttccagctg attgtgcaaa aaggctgtca tctcattcct
      301 gtcgccatgt gctgaaaaag agtccattat ctcaacatcg gccagaatag gctttgtctg
      361 accgtgtaag gtgatggtat ctgctccatc ccggatcaat cggccgggag ttcccaatgc
      421 acaatatccg acaatcaaaa tagttgtact cggcttgtca caattgttga tgatatgatg
      481 ttgtactctt ccggcattgg ccatgccgga cgcgcttatg attacacatg gctcattact
      541 gtcattaatc gccttggaat cctccacatt cttgatatag tccaatccgt tgaacccaaa
      601 tgggttgtca tccaccagaa ggtatttggc catctcatca tcataacatt ccgtatgcgc
      661 tatataaatg gatgtggcat tgactgccag tggactatca acatatacct tgacttttgg
      721 cattctgccc tcattaaaca tatgatccat catgtagatg atctcctgag tgcgacccac
      781 agagaatgaa ggaataatca atttaccacg cttttctatg caggtatgcc gtatgattct
      841 ttccaatttc tctttatcct ggggtgcttt ggcatgtaat cgatctccgt aggttgactc
      901 cgtgatgact atttcagccg gatccattgg taccggatcc cttagtatgg gccgttccgg
      961 ccttccaata tccccggtga atgcaagtgt cctcacctta cccccttcat gaatacgaat
     1021 gttaactgca gccgatccaa gaatatgacc ggcatcggta tatttcacct ctacatcttc
     1081 atggatttta aaccatttgt catatccgta ggaaatcatt agtttcaaca caggaggtac
     1141 atcggtttct acatatagag gatcggtgat ccgactatga tatttaattc ctttttgcaa
     1201 ccatttctta ttagaatatt ccgcttcccg ttcctggatc attgcagaat caaccaatag
     1261 aatacttgaa agtgagcggg tcgcatgggt acaatatata ttaccggtaa acccatcttt
     1321 caccaatcta ggcagtcgac ctgtatggtc aatatgcgca tgggacagaa ctacacaatc
     1381 aatggaggaa ggaggcctta gccacgtttc attgaatttc ttcatgtcgt catcatgtcc
     1441 ctgatacaag ccacaatcca acataaaagt gaaaccattg tccagttcca caacatggct
     1501 actaccggtc acttccttat cggcaccaca aaaatgtatc ttcattattc cctattttaa
     1561 ggcaagttaa ggtaaaatca tcaaaaaaat ggaaataata attgtcaccc ataatcaaag
     1621 cacgagaaca atggccataa aatcattcat aaccacatgt attccttata ataatatatt
     1681 ttatttttta ataacttata atgtttgcca aaagttttat ataccattag caacatagtc
     1741 aaaagttaaa ttggagcaga accactcgac aaagaaaagg catgcgcagg agaagtgaca
     1801 aaacatgaag aagaggaatc acaattaaga agggccaata ctagtttggt aattatcttt
     1861 tacactatta aggatgacat gtgcatcgat caaaaagcct gaacaaagct accccaccgg
     1921 ctgtcgtcat atttctgata aagttcagtg cacatttgtc caaaaattcc actattagaa
     1981 agaaaagcat catcgatcca tcgaaaaaaa tcaattttta gtaatgaaat aaagtgcatt
     2041 tttgttgtag ataaaaagtg tgtagtgatg ttgggacgac taaaaaaaat gcttggtatt
     2101 gatggactgc gggttgattt gattacttca gagacttatg atatcgaaaa gggatatgtc
     2161 gatggagagt tgagtctact ggcaaaatat gatgtcgaga ttgaatacat agagatacgg
     2221 atcattgaaa tatacactcg cggccgtttt ggtaataaaa agactgacga atatcaaatt
     2281 gggactatcc ggacaggctt cggtgtcaac cttggggcca acaggattct gcctgtacca
     2341 tttaaggtgc ctgtagatta tctattctcc tccgttgaca aattcggaat gcttaacatt
     2401 tttaccagag gattggcaaa aggcgcaaaa ctattacgcg gagtccgatc caagtatcgg
     2461 atggaaacgg aagtaaagat agacggatca aatatcaagg tcttcaacag caagcctctg
     2521 atgatcgttt aaggaaatat ttattttgct tgatgaaaaa gatttatccc tcacggtata
     2581 tatcctgtga tcaactccat tgaatacatt taaaaggtaa atcttgctta aattttgaag
     2641 gctgttaata ataaatttct gccatttaca agaataaaga tttactcccc ttatttgtta
     2701 aaataacact atacgaagct tcccgtatat tgttttatta tttatcttta caaataaaca
     2761 aagtataagg aaattcaatc ctgaaaacag gaatgatgta ttaagccgga gagtatgtta
     2821 tctttatttg tattagtcta acctttttca ataatgacaa aagtaccacg ttcaaaagtg
     2881 gtcaggaaag cgcctaacct ataccatgac aatagtcatg atgaagacaa tgcatggaac
     2941 ggtttgccta tgatggctcc gcctactttt cacttacatg caaaaggaac atattcatct
     3001 ttggcaaaca aggatacaaa gccattacag ttaaaacctg cttcagaaca aaaaagtaaa
     3061 aaaactccag acgaaaaaat accttataag aatcaaggac tttcagcaat aaaagacctt
     3121 ttcgagcatg atgctaagat attagctcat cataatctca aactcagtga aaatcatatc
     3181 ataaaagaaa agaacaagta ccaaaacgac cgctatggct accgaaaaga gcattcgaat
     3241 atagccaaaa acctggagca attgatgaaa acccaagcac agattgagca atatcgctat
     3301 cctggttcaa atgtgacttt ttcaaacgcg cgtccgcctt acaaggacca attcttaatg
     3361 gtattgggaa gagcgacttt tagaattaat gaagaaggat tcaagaaggc aaaggcaatt
     3421 tatcccagac ttcaaaactt atattccttt attgatctgc ttattgaaaa attgatttca
     3481 aataatgtat tcttgacggg attgcatgca aggggaaagg tagaggagta taatcaaatg
     3541 agtggtttag accagctggc tgcaattcag gagattttga atgacatcga ctttaatatc
     3601 aagggggccc gggaaaaaat catatccggg gagctgagtg tttacgacct gagtccggtc
     3661 catcaacaaa tggctagctc tcccaaagca gcaggtcaat atcgggactg gaggacaccg
     3721 ggcatgagag acgcaatgca aacaatccta caagaaaaaa agaccagtga cttttggaaa
     3781 gggctgggat tagatgcctt gacagtaatg cttgcagttg gtgcgggatt tgccaatccg
     3841 gtggcagcga tagcaatgat aagcagcaat atagctattt ccggagcaaa ggcaaagtca
     3901 gcctggg
//
LOCUS       DAOQQN010000310         3900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_11242_length_3900_cov_4.715475, whole genome shotgun sequence.
ACCESSION   DAOQQN010000310 DAOQQN010000000
VERSION     DAOQQN010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11242_length_3900_cov_4.715475"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PLG24_10710"
     CDS             <1..328
                     /locus_tag="PLG24_10710"
                     /inference="COORDINATES: protein motif:HMM:NF040323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HMZ25406.1"
                     /translation="KAVEFYVDWLGFKIDWEHKFGENSPIYMQVSRDGIVLHLSEHHG
                     DCAPGSKAFITIDDVRSLHKEICAKEYKYNRPGLEQAFWNAPSFEVIDPFSNKLLITE
                     KSKDDN"
     gene            318..533
                     /locus_tag="PLG24_10715"
     CDS             318..533
                     /locus_tag="PLG24_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HMZ25407.1"
                     /translation="MTTEIILLSTITCPNCGHKKQEVMPTDACQYFYDCENCHAVLKP
                     KAGDCCVFCSYGTVKCPPIQQGQKCCS"
     gene            552..2921
                     /locus_tag="PLG24_10720"
     CDS             552..2921
                     /locus_tag="PLG24_10720"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ25408.1"
                     /translation="MIRSFFLLSVRNLFGRNKAFTIINICGLTVGFATILLVALFIHD
                     EYSFDRFNTKADRVQRIILDFTSEEGNTVQWARTSAPIGKYLAGAFPEVEQVVRLRKN
                     PGTDLLVNDEIKFYEERVFFADSSLFKVFDVKLKTGNPDKALTDKNSIVVTEELAHKY
                     FGGSDPIGKTLRLNNKTDLKVTGVIEAMPGNSHFIADAFVTFSTLDDFLGEKRLTHWG
                     WMDHYTYVLLTDGSTPEQLEKKLPELIKKNAPEWVAENEKLYLQPLTSIHLHSDRKDE
                     VTPNSKESYSYILGTIAVFILLMACANFVNVSTATLVSRFKEISIQKVLGASKLHLVL
                     YFWIESSLMCIVALVVAYLLALLALPYFSQVTGKYISLLDNLWLITPSLLLTLFIGLL
                     SSIVPTIQAATLNVLKIGKSYGESLGKSTIRTILITFQFAISILLITATWIVSSQFSF
                     LKSSRFGFASDNVITIPVKDRSQNDRHQTISKKIGELTGVEKVSYSSSMPGANNAYTY
                     TYSFMGTDVGEQTMAAFLVDEFFFELYEIKLKEGRLPNIESKDTVTEVVLNEAAVEQF
                     HLSSPIGQLVTGQVKGKVVGIIENFNYASLHSSIEPMILYSYPANFRFVSIKLNQGSF
                     QNVISSLDKKWQELYPGYPLEYTFLNETIQKLYGAEFQLSKAYTVFSLIAVIIAGVGL
                     IGLATYLLTRKLKEISIRKVFGSSVGRIVLWIYSGYLKIIIIASVVAWGVGYYVMQNW
                     LDGFAFKTNLEVYYFVIPPLIMTLLLLVATGFQSLKAAGTNPVENLRNE"
     gene            complement(3139..3750)
                     /locus_tag="PLG24_10725"
     CDS             complement(3139..3750)
                     /locus_tag="PLG24_10725"
                     /inference="COORDINATES: protein motif:HMM:NF009949.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="master DNA invertase Mpi family serine-type
                     recombinase"
                     /protein_id="HMZ25409.1"
                     /translation="MIYGYIRVSTDKQTVENQRYEVNQFCEKSVLVVDKWIEETISGS
                     KHIQERKLGKLLKKMRKDDILICSELSRLGRNLLMIMAILNECMNRDIQVWTIKDNYR
                     LGSDINSKVLAFAFGLSAEIERNLISQRTKEALARKKAEGVILGRPIGRKSSRTKLTG
                     QEKKIKELLDKKVSYSAIARILGVHRLTVSGFVSRSGLMECSQ"
BASE COUNT         1109 a          773 c          797 g         1221 t
ORIGIN      
        1 gaaggcagtt gaattttatg ttgattggtt aggtttcaag atcgactggg agcataagtt
       61 tggtgagaat tcaccaatct atatgcaggt gtcgagagat ggcattgttc tccatctttc
      121 cgagcatcat ggtgattgtg cacctggttc taaggccttc attactattg atgatgtccg
      181 ctccctgcac aaagagattt gtgcaaagga gtataaatat aaccgacctg gtttggagca
      241 agctttctgg aatgctccta gtttcgaggt gatagatcct tttagcaaca aattattgat
      301 aactgaaaaa agtaaagatg acaactgaga taattcttct ttcaacaata acctgtccga
      361 attgcggcca caaaaagcag gaggttatgc cgaccgatgc ctgtcaatat ttttatgatt
      421 gcgagaattg ccatgctgta cttaagccaa aggccggtga ctgttgtgta ttttgctctt
      481 acggaacagt aaaatgccca ccgattcagc aagggcaaaa gtgctgttca tagattgaag
      541 atcgaaataa tatgattcgc tcctttttct tgttgagtgt tcgaaatcta tttggcagga
      601 acaaagcatt tacaataatt aatatctgcg gattgaccgt tggattcgcg accattctgc
      661 tggtagcttt attcattcat gacgaataca gctttgacag attcaacaca aaagctgatc
      721 gagttcagcg catcatcctg gactttacga gcgaagaagg taacacagtc caatgggcaa
      781 gaaccagtgc gcctattggt aaatatttag caggagcttt tccagaagta gaacaagtgg
      841 tacgtcttcg taaaaacccc ggaactgatc ttctggtgaa tgatgaaatt aaattttatg
      901 aagaacgagt attttttgcc gatagctcat tgttcaaagt ttttgatgta aaactgaaaa
      961 ctgggaatcc tgataaagca ctaacagaca aaaactcaat tgtagttacg gaagagcttg
     1021 cgcacaaata ttttggcggc agtgatccca tcggcaaaac ccttcgatta aataataaga
     1081 ccgatcttaa agttactgga gtaattgagg caatgcctgg aaattctcat ttcattgctg
     1141 atgcattcgt tacgttctca acattagatg atttcctcgg agagaaaaga ctaacacact
     1201 gggggtggat ggatcactac acatatgtgc tgctgacaga cggttcgaca cctgagcaat
     1261 tggaaaaaaa gcttcctgaa ctgataaaaa agaatgcacc tgaatgggtt gccgaaaacg
     1321 aaaaattgta tttgcagcct ttgacttcca tccatctgca ttcggataga aaggatgagg
     1381 tcacacccaa cagcaaggag agttactcgt acatacttgg caccattgca gttttcatcc
     1441 tactgatggc ttgcgccaat tttgtaaacg tatctacagc aactcttgtg tcgcgtttta
     1501 aggagatctc tattcaaaaa gtattaggag caagtaagtt acacctggtg ttgtatttct
     1561 ggatcgaatc aagtttgatg tgcatagtgg cgctggtcgt ggcttatctc ctggctttgc
     1621 ttgcattgcc ttacttcagc caagtgaccg gaaaatatat ttcattgctc gataatcttt
     1681 ggctgataac tccttcgtta ttgctaacgt tattcatcgg gctgttgtca agtattgttc
     1741 ccactatcca ggccgcaacg ctgaatgtct taaagattgg aaagtcttat ggcgaatcgt
     1801 taggcaagtc tactattcga actatattga tcacttttca atttgccatt tcaattttac
     1861 tgattacagc tacgtggatt gtttcatctc agttttcttt tctgaaatcg tccaggtttg
     1921 gatttgctag cgataatgtc attactattc cggtgaaaga caggtcgcaa aacgaccggc
     1981 atcaaactat atcaaagaag attggtgaat tgacaggagt ggagaaggta tcttattcat
     2041 caagtatgcc tggggcaaat aatgcctaca cctatactta ttcatttatg ggtactgatg
     2101 ttggtgagca gacaatggca gcatttctgg tcgatgaatt tttttttgaa ctctatgaga
     2161 tcaaattgaa ggaaggccgt ctgccaaata tagagagtaa agacaccgtt acagaagttg
     2221 ttttgaatga agcggctgtt gagcaatttc acctttcgtc accaattggt cagttagtca
     2281 cggggcaagt aaagggaaag gtcgtaggaa tcattgaaaa ctttaactac gcatcgctcc
     2341 attcaagtat cgaacccatg atattgtact cataccccgc caacttccgt tttgtttcta
     2401 taaagctcaa tcaaggctcg tttcagaacg tgatttcatc actcgacaaa aaatggcaag
     2461 aactgtaccc tggctatcca ctggaataca ctttcctgaa tgagactatt cagaagttat
     2521 atggagcaga atttcagcta agcaaagcgt acacagtttt ctcgctcatc gctgttatca
     2581 tagccggagt tggattgatc ggattggcta cttatttatt gacacgcaag cttaaggaga
     2641 taagcataag aaaggtattc ggtagctccg ttggccgtat tgtgctatgg atatattccg
     2701 gctacctgaa aattataatc atcgcatcag ttgtagcctg gggtgttggc tattatgtaa
     2761 tgcaaaactg gctggatggc tttgccttta agacaaacct tgaggtttat tattttgtaa
     2821 ttccgccact cattatgacg ttactgctct tagtagcaac tggtttccaa tcgctaaagg
     2881 ccgctggtac gaatccggtt gaaaatttga gaaacgagta atgtgatagt ttgcttctcc
     2941 gctcattgaa aatagaactt agaaaagaat ccttggtttt atgaccgaac gattcttttc
     3001 tgatagaaaa atattgactt atctgctcaa ccgttgcttt gaccggatct tcctgcaccc
     3061 gatcaacgtt gctaaagcaa atcctgtcca gttaccgtat cctatcattg atcattggaa
     3121 ttgaacctat taggtatctc attgactgca ttccattagc ccgctcctac taacaaagcc
     3181 cgatactgta agcctgtgga cgcctaatat cctcgctatt gcactatatg atactttctt
     3241 atctaataat tcctttatct tcttttcttg ccctgtcaat tttgtcctgg atgatttcct
     3301 cccgattggt ctgcctaaaa tgacaccctc tgcttttttc cttgcaagtg cttctttagt
     3361 tctttgcgag attagatttc gttctatctc tgctgaaagt ccgaacgcaa aagcaagaac
     3421 ttttgagtta atgtcactgc ctaaacgata attgtcttta atagtccaaa cttgaatatc
     3481 tcgattcatg cactcgttaa ggatggccat tatcataagt aagtttcttc ccaaccttga
     3541 caattctgaa cagataagta tatcatcctt tcgcattttt tttaaaagct tgcctaattt
     3601 tctttcctga atatgcttcg aaccagaaat agtttcttca atccatttgt caactaccaa
     3661 cacgcttttt tcgcaaaatt ggttgacttc gtagcgttga ttctcaactg tttgtttgtc
     3721 ggtgctgaca cgaatgtagc cgtagatcat agtgtttaag gtttaagttt attagtgggg
     3781 aaagtgtata atttaaacca aaacaggcta tagaaaacct gagcattttt ttatacaact
     3841 aaaccagcgt ttttgttaga caacatggta gtcttagcgg attcacttcg agccaaagct
//
LOCUS       DAOQQN010000311         3889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_11289_length_3889_cov_5.072509, whole genome shotgun sequence.
ACCESSION   DAOQQN010000311 DAOQQN010000000
VERSION     DAOQQN010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11289_length_3889_cov_5.072509"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PLG24_10730"
     CDS             complement(<1..331)
                     /locus_tag="PLG24_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HMZ25410.1"
                     /translation="MRLFFKSIFLNNRFFYAGGVIVVIMFLGFFWPLLIGPAFTLMGL
                     LGLATLMDVFLLWRKSGITASRIMSEKFSNGDENIVKIPLVNKYGMDIGIRIIDELPE
                     QMQERNFK"
     gene            complement(336..1319)
                     /locus_tag="PLG24_10735"
     CDS             complement(336..1319)
                     /locus_tag="PLG24_10735"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HMZ25411.1"
                     /translation="MEENLMEFHSRIDLAGVTDKLNRIKVEIGKVIVGQERNIDLILV
                     ALLANGHVLLEGVPGVAKTLLAKLFARTISAGFSRVQFTPDLMPSDILGTSVFDLKTS
                     AFTFKKGPVFSNIILIDEINRAPAKTQAALFEVMEERQITIDGQQFRMEPPFLVIATQ
                     NPVEHEGTYRLPEAQLDRFLFKIEMLYPELNEEVEILRKDHHNRAEQKQMVVDPVVTG
                     EEIVGMQQVIQGVIVEDNLLEYIATITKATRENQSLYLGGSPRASIALLKASKANAAL
                     HGRDFIMPEDIKMVAAPVLQHRVVVAPAQEMEGVTPAMIIKRIVDSIDVPR"
     gene            complement(1326..2510)
                     /locus_tag="PLG24_10740"
     CDS             complement(1326..2510)
                     /locus_tag="PLG24_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25412.1"
                     /translation="MIRRSYIIIFMAAMIVAFLVARNTSRKPSKLDWNPYYNISTKKP
                     LGLYVLNKEASNLFSEKTITRVNGTPGSYFNKLIEKKEYAANTFVFIGDEMPWEEEEI
                     ENLLTAVDAGMNGFISLNEFPGSMLEKLELRMQEDNESAFVRLSIEEGDSVNIEPAQS
                     GQFNSYFMSYFLPIGRRSRNVLGRNGRFPNFIKVFYGEGTILLHCSPAVFSNYYLLKS
                     DIKPYTESVLSMLPGKKIVWFTGVQQSNISGSPLSFILANPPLRYALYMIVAGVMLFI
                     LFNAKRRQRVIPVRTPNRNTSVEFVRTIGNLYYQKISIPDLLKMRLGFLKERLKTDYQ
                     MSLDQNDAELIDRLSNKTGKPVDLITGFVDLIRDVEAGPPDSKQMLTQFEQYIYRIFI
                     LK"
     gene            complement(2507..3283)
                     /locus_tag="PLG24_10745"
     CDS             complement(2507..3283)
                     /locus_tag="PLG24_10745"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HMZ25413.1"
                     /translation="MTRHLLILLLFFHCFSLSWAAGTNDGITEEYQEDYTEKKDYSGL
                     FQPDNSDTRAIEKARFDENLPEKYKSIIKSYETAPPKEVRKSWFDNLLRKIFGNLPSI
                     NFLGISKLFQILIYAGIGFLAIYLIRLIISYGFDWPFGTKKVEGPEQLEDTMEVTGSE
                     DLSTAIRKAVSEGNFRLAVRYWYIHLLRFSDRNAIISYHPEKTNSDYVHEITNPDFKA
                     KFTELSRIYDFVWYGELTIGERDYWHIAERFDHLVNMLSA"
     gene            complement(3280..>3889)
                     /locus_tag="PLG24_10750"
     CDS             complement(3280..>3889)
                     /locus_tag="PLG24_10750"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HMZ25414.1"
                     /translation="YNESFVRLILGDDYVNMTLDNIANGNPVAVYKSGSNWGSFLGIT
                     FNNLGVSLKAFIYGITAGLGTVYIALSNGVMLGTFQYMFVREGVFWDSVRGIWIHGSM
                     EIFSIVISIMAGLILGAGILFPGTYSRVQSFRKSFEAGLKIFFSTVPFFIAAGFLEGF
                     MTRYAIEMPAFASFGIILTTLALISYYYLVYPYRLMKKITPI"
BASE COUNT         1183 a          899 c          688 g         1119 t
ORIGIN      
        1 acttgaaatt cctttcctgc atctgttcgg gcagttcatc gattatcctg ataccgatat
       61 ccatgccgta tttgttgacc aatggaatct tcactatatt ctcatcacca ttcgaaaatt
      121 tctcagacat gatacgggaa gctgttatac cggatttgcg ccacaaaagg aaaacatcca
      181 ttagtgtagc caaacccagc agccccatta atgtgaaggc cggacctatc aacaatggcc
      241 aaaaaaagcc caggaacatg attactacaa tcacacctcc ggcataaaaa aaccgattat
      301 tcaggaatat gctcttgaaa aacaatctca ttcagttacc ttgggacatc gatagaatct
      361 acaattcttt tgataatcat agccggggtc acaccttcca tctcctgcgc aggggcgacg
      421 actacacggt gctgcaatac aggcgctgcc accatcttga tatcctcagg catgatgaaa
      481 tcccgtccat gaagtgcggc attggcttta gatgctttca agagggcaat agatgctctc
      541 ggtgaaccac ctagatagag cgattgattc tcgcgggtag ctttggttat tgtggcaata
      601 tattcaagca ggttatcctc cacaattaca ccttgaatga cttgctgcat accgacaatc
      661 tcttcgccgg tgacaacagg atccactacc atttgttttt gttctgctct gttgtgatga
      721 tcctttctaa gaatttccac ctcttcattc aactcgggat aaagcatttc tatcttgaaa
      781 aggaagcggt caagctgcgc ctccggcaga cggtaagtcc cttcatgctc tactggattt
      841 tgcgtcgcaa tgactaaaaa aggtggctcc atcctaaatt gttgtccatc gattgttatt
      901 tggcgctctt ccattacttc aaacaaggca gcttgtgtct ttgcaggagc acgattgatt
      961 tcatcaatca ggataatgtt tgagaacact gggccttttt tgaaagtaaa agccgaagtt
     1021 ttaaggtcaa ataccgatgt tccgagaata tcactaggca tcagatccgg agtaaattgc
     1081 acccttgaaa agccggcact aatcgttctg gcaaataatt tagccagtaa ggttttggca
     1141 acacccggaa caccctcaag taagacatga ccattggcca gtaaagcaac taatatcagg
     1201 tctatatttc tttcctgacc aacaattacc tttcctattt caacctttat ccggtttaat
     1261 ttgtcagtaa ctccggccag atctatgcgt gaatgaaatt ccattaggtt ttcttccatt
     1321 gtgatttatt ttaatatgaa tatcctgtaa atatattgtt caaactgagt cagcatttgc
     1381 tttgaatccg gaggaccggc ctctacatct cgtatcaagt ccacaaaacc tgtaatcagg
     1441 tcaacaggct ttccggtttt gttggaaagt cgatcgatca actcggcatc attttggtct
     1501 aatgacattt ggtaatccgt cttcagtctt tctttcagaa atccaagcct cattttcaat
     1561 aagtctggta ttgaaatttt ttgataataa agattgccaa tggtccggac aaattcaacc
     1621 gatgtattac gattcggtgt cctgacagga atgacccttt gccttctctt ggcattgaac
     1681 aagataaaga gcatcactcc ggcaacaatc atatacaatg cataccttaa tgggggatta
     1741 gcaaggataa atgaaagcgg cgaacctgaa atattggact gctgtacgcc cgtaaaccac
     1801 actatttttt ttcccggtag catacttaat acactctctg tgtatggttt tatatctgac
     1861 tttaacagat aataattgga aaaaacagca ggagaacaat gcaacaatat agtcccttca
     1921 ccataaaaaa ccttaataaa attgggaaat ctaccattgc gcccaagtac attccttgat
     1981 ctcctgccaa tgggcaaaaa gtaactcata aaataagagt tgaactggcc tgactgtgca
     2041 ggttcaatat tgacactatc cccctcttct atgctcaacc tgacaaatgc cgattcattg
     2101 tcttcctgca tacgcaattc cagcttttca agcatactac ccggaaattc attcaggcta
     2161 atgaagccat tcataccagc atccacagca gtcaacaaat tctctatctc ctcttcctcc
     2221 cagggcattt catcacctat gaatacaaaa gtattggcag catactcctt tttttcaatg
     2281 agcttgttga aataactccc gggagtacca tttaccctgg taatggtttt ttccgaaaac
     2341 aaattacttg cttccttatt gagtacatat aatcccagag gcttcttagt actgatatta
     2401 taatatggat tccaatccaa cttagaaggc ttccttgatg tattcctggc cacaagaaaa
     2461 gcaactatca tagctgccat gaagatgatg atataagaac gcctgatcat gcagaaagca
     2521 tatttaccag gtgatcaaaa cgttctgcaa tatgccagta gtcgcgctct ccaatcgtaa
     2581 gctctccata ccacacaaaa tcataaattc tggataactc tgtaaatttt gccttaaagt
     2641 ctggatttgt gatttcatga acataatccg aattagtctt ctccggatga tatgaaataa
     2701 tggcgtttcg gtcggaaaac ctaagcaaat gaatatacca gtaccgtacc gccaacctaa
     2761 agttaccttc actaactgct ttacggattg ctgttgaaag atcctcactc cctgtaactt
     2821 ccatagtatc ttccagctgt tcaggacctt caaccttctt tgtcccaaat ggccaatcaa
     2881 atccgtacga aataatcaac cggatcaaat aaatagcaag aaagccaatc cccgcatata
     2941 ttagtatctg aaacaacttt gaaataccca agaaattaat agaaggcaaa ttaccaaata
     3001 tcttccttaa caaattatca aaccaggatt tcctgacctc ttttggaggt gccgtctcat
     3061 aagacttgat aatgctttta tatttttcag gtaaattctc atcgaaacga gccttttcta
     3121 tagcccgggt gtcagaatta tccggctgaa acaatccact gtaatccttt ttttctgtat
     3181 aatcttcctg atattcctca gttattccat cgttggtacc agcagcccaa gaaagactga
     3241 aacaatggaa gaataatagt aaaatcaaga ggtgtcgtgt catattgggg tgattttttt
     3301 catcaatcta tatggataga cgagataata atatgaaatc aaggctaagg tagtcaggat
     3361 aataccaaaa cttgcaaaag ccggcatttc aatagcatac cttgtcatga agccttctaa
     3421 aaatcccgca gcaataaaaa atggcactgt gctgaaaaaa attttcaacc cggcttcaaa
     3481 tgactttcgg aaagattgta ccctggaata ggtccccgga aacaatattc ctgcaccgag
     3541 tataagaccg gccataatgc ttattacgat tgagaatatc tccattgatc cgtgaatcca
     3601 aatacctctg acactatccc aaaacacgcc ttcccgaaca aacatatact ggaaggttcc
     3661 cagcataact ccgttggaca gcgcaatata gacggttccc agtcctgcag tgatgccata
     3721 aataaaggcc ttgagactga ctcccagatt attaaaggtt ataccaagaa agcttcccca
     3781 gttgcttccc gacttatata cagccaccgg attgccattg gcaatattgt ccaatgtcat
     3841 attgacataa tcatcgccga gaataaggcg tacaaagctt tcattgtat
//
LOCUS       DAOQQN010000312         3887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_11302_length_3887_cov_5.022443, whole genome shotgun sequence.
ACCESSION   DAOQQN010000312 DAOQQN010000000
VERSION     DAOQQN010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11302_length_3887_cov_5.022443"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2304)
                     /locus_tag="PLG24_10755"
     CDS             complement(<1..2304)
                     /locus_tag="PLG24_10755"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ25415.1"
                     /translation="MINRIVPIIGTLFFVTPLMYAQSVVLHPAYQGIGIEVDLPAGFD
                     DDNSTYCTIKYKPADTRTWKDAFPPDRITFSGQDQFRGSVFLLQAGTPCDIAVSIHDS
                     IPEEKVIYQHTVSTSTLNDPETIVGTEIRWVAPDGSGKEYTKDKPGQLKTLLESGDVT
                     CGTTIYLMDGVYTDLSIVFRLNSSCTEEKPIHIMAAPGAHPIIDGAYQGTLAWTRNEH
                     DTLLYRSNLPPNTGYTNICILNGNNLYPYPTLNAFWYFHDHNLRDLNFNADGFVRDES
                     NIWIKTKAGTNPNNTDVTISKAFRFITVYGNSHDGYLHFKGLTIKNIAKNQKESQSMA
                     FDLRDVNHIWFDSCQFVFNNFDIQFGGKCDHILIENCQFKNDNGLYSHVMIKRSPLYF
                     ITEATSYARNRETGALVFKNNSNVTIRNNVFTGTNSGMYLNSSAGVAEADVYDNEFVD
                     NYDAIECDGNWVNFRVLNNRFVRPMAGISAAPPGIGPRYFYRNVFYGMRGRENVKDDP
                     YFVGCTPPTVYYSPATGIKTNSSGTNPARGNMYFFNNTFYSDDTLAFTMEPWLGEWKN
                     IYFVNNIFSDNIKHPYYFHGLKDKPDFQFSSDHDNYYSYDESSPLILAKEMHGKYNCH
                     SWFEVDSFQSQMRALTGSNRIFFNKPYHQNPAFKDVSHGDFSLEDYSPLIDGGVIIPG
                     FYDFQGSVPDIGAIESNKSSAVYESSTPQNTFTLYPNPTTGTVYMKGRNNEIPAFVQV
                     WNIQGINTGIEYNPGSNSIRLGHLPPGM"
     gene            2826..3035
                     /locus_tag="PLG24_10760"
     CDS             2826..3035
                     /locus_tag="PLG24_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25416.1"
                     /translation="MDGLPDALPDLSNRVEGWEATYYPCPIAYRNIWTPAGNERFEKI
                     GGEEDRPRLICVFDRLCIQGAGRRM"
     gene            3357..>3887
                     /locus_tag="PLG24_10765"
     CDS             3357..>3887
                     /locus_tag="PLG24_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25417.1"
                     /translation="MRILYTFVFMFMMMVGQIGAQTIIIGTGGSNTDGYGNDPIDDYF
                     MYMRYQTVYTATELSTAGLTPYDILTGLGFSVSQSPGTLANYTIRIGHTTATNAAAHI
                     SAASTEVKSAFSYTPTVVSAGSFDMITFSTNFVWNGTDNIFIDICTGSNTFASPYGGV
                     RVHTLTSGSRYVRSDGS"
BASE COUNT         1126 a          793 c          852 g         1116 t
ORIGIN      
        1 catgccggga ggcaggtgtc ccagcctgat actattactc ccgggattgt actcaatgcc
       61 cgtattgata ccctggatat tccacacctg gacaaaagcg ggaatttcgt tattccggcc
      121 tttcatgtat acagtacctg tcgtaggatt cggatagagg gtaaatgtat tttgaggagt
      181 ggaagattca tagaccgccg aggatttatt tgactctatg gccccgatat caggaaccga
      241 gccttgaaag tcataaaaac cgggaatgat gacgcctcca tcaatcaatg gactgtagtc
      301 ttccaacgaa aagtcaccat ggctgacgtc cttaaaagcg ggattttgat ggtaaggctt
      361 attaaagaat attctgttgc ttccggtaag tgcacgcatc tgtgactgaa aagagtcgac
      421 ctcaaaccag ctgtggcaat tatatttgcc atgcatctct ttagccagga ttaaagggga
      481 ggattcgtca tagctgtaat aattatcatg atcagacgaa aattgaaagt ccggtttatc
      541 tttcaatcca tggaaataat aaggatgctt gatattgtcc gaaaaaatat tattaacgaa
      601 gtagatgttt ttccattcgc ccaaccaggg ctccatcgtg aaggccaggg tgtcgtcaga
      661 gtaaaaagta ttgttgaaaa aatacatatt tcctctggcc ggatttgttc ctgaagagtt
      721 ggtctttata cctgttgccg gtgaataata cactgtgggc ggggtacaac ccacaaagta
      781 tggatcatcc tttacatttt cccggccccg cataccatag aatacattcc tgtaaaaata
      841 gcgcggccca ataccaggcg gtgccgcact aataccggcc atcggacgta caaatctatt
      901 attcagcacc cggaaattga cccagttgcc atcacactct atagcgtcat agttgtcgac
      961 aaattcattg tcatagacat cagcctcagc tacaccggca ctgctgttga gatacatgcc
     1021 actattggtc cccgtgaata cattattacg gatggtaaca tttgaattgt tcttgaatac
     1081 gagggctccg gtctccctgt ttcttgcata ggaagtggct tctgtgatga agtacagggg
     1141 agatcgtttg atcattacat gactgtacaa gccattgtca tttttaaact gacaattttc
     1201 aatcaaaata tgatcgcact tgcccccaaa ctggatatca aaattattga aaacaaattg
     1261 acaggagtca aaccaaatat gattgacatc cctgagatca aatgccatag actgactttc
     1321 cttttgattc ttagcgatat ttttaatcgt caatccttta aaatgaaggt acccgtcatg
     1381 gctgttgcca tagaccgtaa taaaccgaaa tgcctttgag atagtgacgt ctgtgttgtt
     1441 ggggtttgtt ccggcctttg tcttgatcca gatattactt tcatccctta caaatccatc
     1501 tgcattaaaa ttcagatctc ttaaattgtg atcgtgaaaa taccagaatg cattcagggt
     1561 gggatatgga tacaggttgt tgccattgag gatacagatg ttggtatatc ctgtgttggg
     1621 aggcaaatta gagcggtata gaagcgtgtc atgttcattt cttgtccagg caagcgtgcc
     1681 ctgataggcg ccatcgataa tgggatgcgc gcccggagct gccataatat ggatcggttt
     1741 ttcttcggta caggagctgt tcaatcgaaa aacgatcgaa agatcagtat atacgccgtc
     1801 cattaaataa atggtcgtgc cacaggttac atcccccgat tccagcaacg tctttagttg
     1861 tcccggctta tcctttgtat attctttgcc ggaaccgtcc ggagcgaccc atcttatttc
     1921 cgtacccaca attgtctcgg gatcattgag ggtcgaggta cttacagtgt gttgatatat
     1981 aactttttcc tcaggaatac tatcgtggat actcaccgct atatcgcaag gggtgccggc
     2041 ctgcaacaaa aacacacttc ccctgaattg atcctgaccg gaaaaagtaa ttcgatcggg
     2101 gggaaatgca tccttccagg ttcttgtatc ggccggctta tattttatgg tgcaataggt
     2161 cgaattatcg tcatcaaaac ccgccggcaa atcaacttca ataccgatac cttgataggc
     2221 tggatgcaat actacggact gggcatacat caggggcgtt acaaaaaaaa gtgtcccaat
     2281 tattggtaca atacgattta tcatctgaat attttttggg gttaaaaaat atggatgtat
     2341 aaataatgga tgactaaagt aaaagtattc agccaaaaga aaaagttaaa aagttatata
     2401 acaataaatg attgtgtgta aaataatgat attgtgcgat atagctcatg tcggtctccc
     2461 ttaccttccc gggatggctt caatataata ggttataagg aaggatgtgg gaggcgcttt
     2521 ttttcggtct ttgaaaacaa agcaggatca acttcctttt ttaaaaatct tcataataag
     2581 tccccggata ttatgcatac aatcggttga gatgctgacc gggactctac gaaggctcag
     2641 attatataac cgggatttac cggcaggaat gcagagcccg ggtcaggagt atcccggggc
     2701 tttgcaagtg gtctgcacca ttaaaaaaag aataacggga caaaacgcaa ttgttcaaat
     2761 cccatgctat cgtatatacg ccaacttgaa aatagcaact tacggacaga ccatcttgat
     2821 cttccatgga tggccttccg gatgcattgc cggatctttc taatcgtgta gagggatggg
     2881 aggcgactta ttacccctgt cctatcgcct atcgaaacat atggactccg gcgggcaatg
     2941 aaagatttga gaaaatcggt ggggaggaag accgcccccg gctcatttgt gtttttgacc
     3001 ggttatgcat tcagggcgcc ggtagaagga tgtaggtcgc cccggtcaaa acaagcgccc
     3061 gcctccgtgt aaaatttcgc caattgaaca atattttaat aataatgaac atattttaca
     3121 aaaatccgaa taaaatttta taaaaattac acggcaaaaa acatacaacc tgagcattta
     3181 tggcttatat ttgccggttc agggagggtc cggaggactc cgcataagga tgcttccgcc
     3241 cgataagact tcacaggtca caagaataag aaagaataga aatacccttt tattgaatca
     3301 gggagattgc cgaaaatcct gtaaagagta ggcgttaatt tttaaatatt taaagtatga
     3361 ggattttgta cacttttgtt tttatgttta tgatgatggt gggtcaaata ggtgctcaga
     3421 ctattatcat tggaacagga ggatcaaata cagatggata tggtaatgat cccattgatg
     3481 actatttcat gtatatgaga tatcagacag tttatactgc aactgagctt agtactgctg
     3541 gtcttactcc ttatgatatt cttacaggat tgggtttttc tgtttcacaa tcgcccggta
     3601 cacttgctaa ttatacgata agaatcggac ataccacagc tactaatgct gctgctcaca
     3661 tttcggcagc aagtacggaa gtaaagtcag cattttctta cacaccaact gtagttagtg
     3721 cgggcagttt cgatatgatc accttcagca ccaattttgt ctggaacggg acagataata
     3781 tttttataga tatctgtact gggtcaaata cttttgccag tccatacggt ggagtaagag
     3841 tacatacgtt aaccagtggt tctcgttatg tacgtagtga tggttct
//
LOCUS       DAOQQN010000313         3875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_11356_length_3875_cov_5.017277, whole genome shotgun sequence.
ACCESSION   DAOQQN010000313 DAOQQN010000000
VERSION     DAOQQN010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11356_length_3875_cov_5.017277"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PLG24_10770"
     CDS             <1..663
                     /locus_tag="PLG24_10770"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HMZ25418.1"
                     /translation="AKADFNLRFAIYTFTSDALANAIIERKKANLIIRGITDNNTDNS
                     GKLTYLQQNGVDVIDHLPSSLMHHKYAVLDAEYQDSDPVVITGSHNWTWSADNINDES
                     TMFIHDMDIAKLYVAEFNSRYCELAPWDCNLLDVKEYVNADIKIFPSITSGLVQLQSN
                     GNNRITSIFIADQIGRPVYYTKSTLEMNLHMDISFLQPGIYFITVSGEQAGYATSKII
                     KI"
     gene            complement(683..1798)
                     /locus_tag="PLG24_10775"
     CDS             complement(683..1798)
                     /locus_tag="PLG24_10775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ25419.1"
                     /translation="MKRGNKIILLTFGDILFDQRLQKSIRSLEQAGYEIIVFARKLFP
                     ESPDRNIHALRYRPLFNRGVMAYLEYNVRAFLKLVFSRHHVVVSFDLDTLPAAIAASY
                     LKRLVVIHDAHEFFVESPELVLRSKVRAIWERIACFCIPKVDRAYTVGNAIAQAMQEK
                     YGIPFEVIRNVPMVGDANEMKGEGSEIKFPKPYIIYQGALNTGRGLEQIIQVMKHFPD
                     INLVLAGRGDIELYLKEMAVSQTLSNIYFVGNLRPDELKIWTKKAIFGLNLLENMGKS
                     YFLSLANKFFDYIEAGIPQICIDFPEYRALNSRYDVAILVESCDEHLLLEGFNILLKD
                     TEVYQRLKSNTAIAIRELNWELESEKLVRLYNSWLSM"
     gene            complement(1795..3699)
                     /locus_tag="PLG24_10780"
     CDS             complement(1795..3699)
                     /locus_tag="PLG24_10780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HMZ25420.1"
                     /translation="MVSAQEILKKYWNYNSFKPVQEDIIRHITEKGDAFVLMPTGGGK
                     SLCFQIPALMMKGMTIVITPLISLMKDQVMRLKSLGINAEAIYSGMTKMDIHRILDSC
                     LTGKIKLLYLSPERINTSLFKEFANGLHVDLIVADEAHCISQWGYDFRPAYLKIREAR
                     KIFPKARIMALTASATPEVVNDIMEHLSIGDAAIFKLSFLRSNLSYVCRNTTDKRFEL
                     LNLVRKVPGTGIVYCRNRRATAEIASFLSGHGISADFYHAGLDQKRLSDVQRGWMEGK
                     IRVIVATTAFGMGIDKPDVRFVAHLALPPSPEEYYQEAGRGGRDGKHAYAALFYDDED
                     IHELRESIDSNFPGKEQLVKLYHRLALYFHVGAGPLEEQYFDFDINLFCKEYGYTPVW
                     VVKGLKELERQGHVYVTEGIYLPNQVMILYSKSELDTFRSRQPDLIPILEVLLRLYGG
                     IMSIPVKINIKAIGQKLRTTSENVLAYLNILANEGVIWFQPQKDKPQISFGEFRYVSD
                     SLRFDFSLQEFLKKNYKARVEAMISIVEQEVCRNKTLLSYFGEAYTHNCGICDVCLQE
                     KRIENKDSGLSRTIEYVMNFATSEHNDLQTLLSTTYYRANKEKVDAALQFLVQEGTIV
                     VDNFKIVKAE"
BASE COUNT         1185 a          894 c          661 g         1135 t
ORIGIN      
        1 gccaaggcag atttcaatct aaggtttgct atttatacct ttacttccga tgctttagca
       61 aatgcaatca ttgaaagaaa gaaagccaat cttataatca ggggaattac ggacaacaac
      121 accgacaatt caggcaagct tacttacctg caacagaatg gcgtagatgt tatagatcac
      181 cttccatcaa gcttaatgca ccataaatat gcagtgttag atgctgaata tcaggattct
      241 gacccggtag taatcacggg ctcacataac tggacctggt ctgccgataa tatcaatgac
      301 gagtctacaa tgttcattca tgacatggac attgctaaat tgtatgtagc agagtttaac
      361 agtaggtatt gtgaacttgc cccctgggat tgtaatctgt tagatgtcaa ggaatatgta
      421 aatgccgaca ttaaaatatt tccatccata acctccggtc tggttcagct ccaaagcaat
      481 ggaaataacc ggatcacatc tatattcatt gcagatcaga ttggcagacc cgtgtattat
      541 accaaatcaa cgttggaaat gaatctccac atggatataa gctttctcca accgggaatt
      601 tatttcatta ccgtatccgg agagcaagcc ggatatgcaa caagtaaaat aataaaaatc
      661 tgaattaaaa catattattg gattacattg acaaccaact attatatagt cttaccaatt
      721 tctctgattc cagttcccag ttgagctctc ttatggcgat ggctgtattg gactttaacc
      781 tttgatatac ctcagtatct ttgaggagta tattaaatcc ttccaaaagt aaatgttcat
      841 cgcaactttc aactaaaatg gcaacatcat accgactatt caaagctcta tactccggaa
      901 aatcaataca aatctgtgga atgcctgctt caatataatc gaagaattta tttgccagtg
      961 acagaaaata acttttaccc atattctcca gaagattgag cccgaaaatg gcttttttgg
     1021 tccaaatctt tagttcatcc ggcctgaggt tacctacaaa gtaaatattt gataacgttt
     1081 ggctaacagc catttctttc aaataaagct caatatctcc ccttcccgcc aataccagat
     1141 taatatcggg aaagtgcttc atcacttgaa taatttgctc caatccccta ccggtattca
     1201 aagcaccttg atagataata taaggctttg gaaatttgat ttcagaacct tcccccttca
     1261 tctcatttgc atcacccacc ataggtacat tccgaatgac ttcaaagggg atgccatact
     1321 tctcctgcat ggcttgtgca atggcatttc caacagtata agccctgtca actttgggaa
     1381 tacaaaagca ggcaattctc tcccaaattg ctctgacctt ggatctcaaa accagttccg
     1441 gtgactctac aaagaactca tgagcatcat gaataactac cagtcttttc aggtaagaag
     1501 ccgcaattgc agccggcaag gtatccagat caaagctgac gactacatgg tgccgtgaaa
     1561 atactaactt taagaaagcc cgcacattgt actcgaggta agccatcaca ccccgattaa
     1621 ataacggccg gtatcttaag gcatgaatat ttcgatcagg actctcaggg aataattttc
     1681 gggcaaatac tataatctca taccctgctt gttccaggct tctgatactt ttctgcagtc
     1741 gttgatcaaa cagtatatct ccgaatgtaa gtaagattat tttattgcct cttttcactc
     1801 tgccttgaca attttaaagt tgtcaactac aatcgttcct tcctgaacca agaactgtaa
     1861 cgcggcatca actttttctt tattagcgcg ataataagtt gtggatagta aggtctgcag
     1921 atcgttatgt tctgatgtgg cgaaattcat gacatactct attgtccggg aaagtccgga
     1981 atctttattt tcaatgcgct tttcttgcag acaaacatca cagatgccac aattatgtgt
     2041 atatgcttct ccgaaatatg ataacaatgt cttgtttcta catacttcct gttccacaat
     2101 agaaatcata gcctccacac gagccttgta attttttttg agaaattcct gcaggctaaa
     2161 atcaaaacgc aggctatccg aaacgtacct gaattcgcca aaggaaatct gtggcttatc
     2221 cttctgaggc tggaaccata ttaccccttc attggccagt atattcaaat atgctaacac
     2281 attttcagat gtagtcctca acttttgccc tatggccttt atattgatct tgacaggaat
     2341 actcataatt ccaccatata gcctcaaaag cacctcaaga atgggaataa gatccggttg
     2401 ccgtgaccga aaagtatcca actcagattt actataaaga atcatgacct gattaggtaa
     2461 ataaatgccc tccgtaacgt aaacatgtcc ttgccgctcc aattccttga gccccttcac
     2521 aacccacacc ggtgtataac catattcttt acaaaataaa ttgatatcaa agtcgaaata
     2581 ttgctcttcc agggggcctg cccctacatg aaaatataat gccaacctgt gatacaactt
     2641 cactagttgc tcctttccgg ggaaattgga gtctattgat tcccttaatt catggatatc
     2701 ctcatcatca taaaacaaag ctgcatatgc atgcttgcca tcacgcccac ctcttccggc
     2761 ttcctggtaa tactcctcgg ggctgggagg caatgcaaga tgcgctacaa atctgacatc
     2821 cggcttatca atacccatac caaaagcagt ggtggcaaca atcactctta tctttccttc
     2881 catccaacct ctctgcacat cggataatcg tttttgatcc agtccggcat gatagaaatc
     2941 tgctgaaatt ccatgacccg ataaaaagga agcgatttcg gctgtagcac gtctgttccg
     3001 acaataaact atcccggtcc cgggaacttt ccgcaccaga ttaagtaatt caaatcgctt
     3061 atcagttgta ttacggcata cataggacag gttgctcctc aaaaagctca gtttaaaaat
     3121 agctgcatct ccgatactta ggtgctccat tatatcattc actacttccg gagtagcaga
     3181 agctgtaagt gccatgatac gtgctttagg gaaaatcttc cgtgcttcac gaatttttag
     3241 gtatgcaggc cgaaaatcat atccccactg tgatatacaa tgcgcctcgt cagcaacaat
     3301 caaatcaaca tgcaagccat tggcaaactc cttaaacaat gatgtgttta tccgctcagg
     3361 agatagatat aacaacttaa tcttaccggt caaacaacta tcgagaatcc tgtggatatc
     3421 catttttgtc ataccactgt aaatagcctc agcattaatc cctaatgact taagtctcat
     3481 cacctggtcc ttcatcaacg aaattaaagg cgtaatgaca atggtcattc ctttcatcat
     3541 caaagccgga atctgaaaac acagagactt acctccgccc gtaggcataa gcacaaatgc
     3601 atctcctttt tcagtaatat gtctgatgat atcttcctgt acaggcttga aactgttgta
     3661 attccagtat ttcttcaata tttcctgtgc agataccacg aaagaacttt tattgaacta
     3721 ccaaactgat tataacttta catattaaaa ggtaggacac ttgactttat tgattagttc
     3781 aagcccaaaa aaatcattca tattgaatga tactcccaag agcccggtga agtacatatc
     3841 attcgatttt ggattacctc tttgttggtt gtcag
//
LOCUS       DAOQQN010000314         3855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_11443_length_3855_cov_5.337632, whole genome shotgun sequence.
ACCESSION   DAOQQN010000314 DAOQQN010000000
VERSION     DAOQQN010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11443_length_3855_cov_5.337632"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1059
                     /locus_tag="PLG24_10785"
     CDS             85..1059
                     /locus_tag="PLG24_10785"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HMZ25421.1"
                     /translation="MLELFRRDQPIGYIFLFLYTILLRAPTFISETNWSPLASSAFSN
                     LVYNMTGYTSVAAQVTGTVLIFIHAVLINMMVAENRITRENSLFPGLFYVLFSTLLPD
                     FNYLSPALMANTFIILTLYNIFNSYKKSGNCSEVFNAGIFIGIAMLFDFSYAIFLICG
                     FVGLLIVRSFRFVERIQFLLGFVIPIFLGGTYYFWMGHLDDFLQTFTTNISWLDWAGN
                     INSQFFLKVGILIFLMLFVIFNYSNYGKKNNVHAMRKVDVMFWFLILPVTTFLFQTGI
                     RLDHALIVAPPMGILLGFSVLNIQNKMIAEVVHLVLISISLLFQYILL"
     gene            complement(1158..3038)
                     /gene="htpG"
                     /locus_tag="PLG24_10790"
     CDS             complement(1158..3038)
                     /gene="htpG"
                     /locus_tag="PLG24_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HMZ25422.1"
                     /translation="MEQVEKGQITVNTENIFPIIKQFLYSEQEIFLRELISNAVDATS
                     KMKTLSSKGVAPGELGDLTIEVIPDKEAKTLIIRDKGIGLTAEEAKKYLNQVAFSSAQ
                     EFLDKYKEDANIIGHFGLGFYSAFMVADKVEVDTLSYQEEAQPVVWSCSGDTSFEIYH
                     SQRTSRGTDIILHLSDDAIQYLEEDRLAELLDKYCKFLPIPIQLGMKKKTISEGEGDD
                     KQEREIEVPNIINNTNPLWKKSPSDLKDEDYLAFYNELYPFSRPPLFWIHLNIDYPFN
                     LTGVLYFPKIESNFDIQKNKIHLYSNQVFVTDDVKEIVPEFLMLLHGVIDSPDIPLNV
                     SRSYLQADSNVRKITGYITKKVADKLNELFKQDRPAYEAKWPDIGTFIKYGFLSDEKF
                     EEKVGPSILLKNTEGKYFTLEEYKEKIKANQTDKNGKIVILYSNILNDHHSYIQAALA
                     KGYDVVEMDQIIDNHFIQHLEYKKGEFTFKRVDSDVADHLIEKDETIPEVLTESEIKH
                     VDELFKTVLKESMNKVEVKPLSSGEQPVVIVKPEFMRRMTEMQMLQGNAMGKMPEMYT
                     VIINSNHPLINSKLIKMEDSAEKEKFAHYLYDLARLSQQMLTGEELTRFIKENLEKLS
                     NS"
     gene            complement(3211..>3855)
                     /locus_tag="PLG24_10795"
     CDS             complement(3211..>3855)
                     /locus_tag="PLG24_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25423.1"
                     /translation="WALIPLIGLKDILCGIMILYSDNRWLIYWLIFWALFTALLRPVA
                     GFGMVGYIERAGNYGPAIALLYFLNNIWKKELSAEKLKHHISKLELILRICLSLLIMG
                     HAGLVITDPILTAYGDYKALIANLGVLNYPTDAAWLMALAVFEMTLSLLVLFWPRITG
                     LMLFVLIFKVVSEGMYAFAYGPYSIFETIERFGDYLIPLILMVIYKTWSQEDLA"
BASE COUNT         1050 a          769 c          760 g         1276 t
ORIGIN      
        1 aactgatggt gatgcaccat cgacattcag tatatgctat tcattggaaa aaaattacct
       61 ttgtcccgat aaaaccggct tgtcgtgtta gagttattca ggagagatca gccaattggc
      121 tatattttcc ttttcttata tactattttg ctgagagctc cgactttcat cagtgagacc
      181 aattggtctc ctcttgcaag tagtgcattc agcaatctgg tctataatat gaccgggtat
      241 acatcggtgg ctgctcaggt tacgggaaca gttctgatct tcattcatgc agttctcatc
      301 aatatgatgg ttgcagaaaa caggataaca cgtgagaata gtcttttccc cgggctattc
      361 tacgttttat tctcaacctt acttccggat ttcaactatc tgtcaccggc tcttatggcc
      421 aataccttta tcattttaac tctttataac atattcaact cctataaaaa atccggaaat
      481 tgcagcgagg tgtttaatgc cgggattttc atcggcattg cgatgctatt tgattttagc
      541 tatgccattt ttttaatttg cggatttgtt gggttattaa ttgtcaggtc gttcagattt
      601 gttgagcgga ttcagttttt attgggattt gttattccta ttttccttgg tggaacctat
      661 tatttttgga tgggacattt ggatgatttt ttacagacct ttaccaccaa tataagctgg
      721 ctggactggg caggcaatat taatagtcag ttttttctta aagtcggtat tttaatcttc
      781 ctgatgttat tcgttatttt taattacagc aactatggca agaagaataa tgtccatgcg
      841 atgagaaaag tagacgtgat gttttggttc ttgatccttc ctgtgactac atttttgttt
      901 cagaccggta tccgattaga ccatgctttg atcgttgcac ctccaatggg gatcttactg
      961 ggtttctccg ttcttaatat tcagaataag atgatagcag aagtagttca tttggtcttg
     1021 atcagtatct cattgttatt tcaatatatt cttctatagt taatagcttt tccgggaagt
     1081 attttccggg tagtggataa aaaaatagcc ggggacaaat cccggctaaa tctttttacc
     1141 tgatagatct atcagaatca actgttgctc agtttttcaa ggttttcctt aataaacctg
     1201 gtgagttctt cgcctgtgag catttgctga ctgagtcttg ccaaatcata gagataatgt
     1261 gcaaactttt ccttctcagc ggaatcttcc atcttgatta atttgctgtt gatgagagga
     1321 tggttgctat tgataatgac cgtgtacatt tccggcatct tacccatcgc atttccttgg
     1381 agcatctgca tttcagtcat tcggcgcatg aattcaggtt tgacgatgac aacaggctgt
     1441 tcccctgaag acaagggttt cacttccact ttattcattg attcctttag cactgttttg
     1501 aatagttcat ctacatgctt aatctccgat tcagtcaaaa cttccggaat ggtttcatcc
     1561 ttttcgatga gatggtcagc aacatctgag tcaactcgct tgaaggtaaa ttctcctttt
     1621 ttatactcca gatgctggat gaaatgattg tcgataattt gatccatctc aacgacatca
     1681 tatccttttg ccaaggcagc ctggatatac gagtgatggt cattgagtat gttggagtaa
     1741 agaatgacaa tctttccgtt tttatcggtt tggtttgctt taatcttttc cttgtattcc
     1801 tcaagggtaa aatatttgcc ttctgtattc tttaacaata tggaagggcc aaccttttct
     1861 tcaaatttct catcggaaag aaaaccatat ttgataaatg ttcctatatc cggccacttg
     1921 gcttcgtatg cgggtctgtc ctgcttgaat agttcattga gcttatcagc taccttttta
     1981 gtgatgtaac ctgtgatctt tctaacattg ctgtcggctt gcagataaga tcgcgataca
     2041 ttgaggggaa tatcaggtga gtcaattaca ccgtgcagta acatcaggaa ttccggtaca
     2101 atttccttta catcatccgt tacaaatacc tgattggaat aaagatgaat cttgtttttc
     2161 tgaatgtcga aattgctctc gatcttcgga aaatataaca cacctgtcag gttgaaagga
     2221 tagtcgatat tgaggtgaat ccagaacagg ggtggtctgc tgaatggata taattcatta
     2281 taaaatgcca gataatcttc atccttcagg tcagaaggtg attttttcca taagggattg
     2341 gtattgttga tgatattagg aacctcgatc tccctttctt gcttatcgtc accttcgccc
     2401 tctgatattg tttttttctt catacccagc tgtatgggta tcggcagaaa tttgcaatat
     2461 ttatccagca attccgccag tctatcctct tcaagatatt gtatggcgtc atcactcaga
     2521 tgtaagataa tatcggtacc tcgactggtt ctttgcgaat ggtatatttc aaaacttgta
     2581 tcgccactgc atgaccatac tacaggttgt gcttcttcct ggtaggaaag tgtatccacc
     2641 tccaccttat ccgctaccat gaatgcggaa taaaagccca atccaaagtg gccaatgatg
     2701 ttggcgtctt ccttgtattt atccaggaat tcctgtgcgc tcgaaaaggc tacctgattg
     2761 aggtattttt ttgcttcttc tgctgtgagg ccgatacctt tatcccggat gatgagcgtc
     2821 ttagcttctt tgtcgggtat gacttctata gtcagatcgc ccaattcacc aggagccaca
     2881 ccttttgatg atagcgtctt catcttggaa gttgcatcaa cagcattcga tatcagttcc
     2941 ctgagaaaaa tttcctgttc agaatataga aattgcttga tgatgggaaa gatattctca
     3001 gtattgaccg taatttgtcc tttttccact tgttccatat ttaatttatt ttataagaaa
     3061 atagcatagc aataagcatt ccttgacttg agattatgtc aatttggcaa gttggaatga
     3121 agaaatgaca gatgaaaaaa aataaaaagg caatttatca acaataaatt gccttttcag
     3181 gtgatattta gtacctgatg atctaatgga ttaggcgagg tcttcctgac tccaggtttt
     3241 atagatgacc attagaatca aaggaattaa ataatcacca aagcgctcta tggtttcaaa
     3301 gatggagtat ggtccataag caaaggcata cattccctca ctgacgacct taaagatcaa
     3361 gacaaacaac atgagaccgg taattcttgg ccagaacagg acgagaagac tcagagtcat
     3421 ctcaaagaca gccaatgcca tcaaccatgc agcatcagtc ggatagttca gtacaccgag
     3481 attggcgata agggctttat agtctccata tgcagtcagg attgggtcgg tgatgaccaa
     3541 tccggcatgg cccatgatta acaaagaaag acatatcctg aggattaatt ccagcttact
     3601 gatatgatgt ttcagttttt ccgcagaaag ttcttttttc cagatattgt tcagaaaata
     3661 tagcagggca atggcgggcc cataattacc ggctctctca atatagccaa ccatgccgaa
     3721 gccggctaca ggccttagca aagcggtgaa gagggcccag aatatcaacc aataaatcaa
     3781 ccagcggtta tcactataca atatcatgat accacacaat atatccttca agccaatcaa
     3841 tgggatcaat gccca
//
LOCUS       DAOQQN010000315         3852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_11455_length_3852_cov_5.663418, whole genome shotgun sequence.
ACCESSION   DAOQQN010000315 DAOQQN010000000
VERSION     DAOQQN010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11455_length_3852_cov_5.663418"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLG24_10800"
     CDS             complement(<1..258)
                     /locus_tag="PLG24_10800"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HMZ25424.1"
                     /translation="MNTKIAVIGLGYVGLPLAVELAKHYHVIGFDINKNRLEELRKGK
                     DHTLEVEESNLISVLNQNDQIGLSFTDDAQKIKGFDFYIITV"
     gene            complement(314..1621)
                     /locus_tag="PLG24_10805"
     CDS             complement(314..1621)
                     /locus_tag="PLG24_10805"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HMZ25425.1"
                     /translation="MNISVIGTGYVGLVTGTCFSETGNSVMCVDIDENKVNSLKSGVI
                     TIYEPGLEVIFQRNLEKKRLEFTTDLKKAVDFADIIFLALPTPEGEDGSADLSYVLNV
                     ANQLGKMISSYKIIVDKSTVPVGTADLVHQAIAQNAAAELFDVVSNPEFLREGVAVED
                     FMKPDRVVIGTSSPRAREIIHRLYEPFVKQGNPIYFMDERSAEMTKYAANSFLATKIT
                     FMNEIANLCERVGANVDMVRLGMGADSRIGKRFLFPGIGYGGSCFPKDVKALHKIADQ
                     NDFNFRILDAVIQVNINQRTILFDKLHNFYKGNLNGKTIAIWGLSFKPNTDDIREAPA
                     LYIIDKLLDAGAKIRVFDPEAMDNVRRIYGEKLFYAVDQYEVLSDCDALMIMTEWNEF
                     RSPQFDSMFHSIKDKVIFDGRNIFDPKEMQEKGFNYESIGRPV"
     gene            complement(1760..2275)
                     /locus_tag="PLG24_10810"
     CDS             complement(1760..2275)
                     /locus_tag="PLG24_10810"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HMZ25426.1"
                     /translation="MMNTWVGKQWYAVKVKYKSEKVVIAQLERKDIEAYTPFRRYTSR
                     YLRKIVHRMVPVLSTYVFVHIDRKQYVSVLETEHVFGFVKVGKLLSPVTQAEMDILHR
                     IEGLDEKYAFKEVRYQTGQRVHVNNGALFGMQGIVSEVKSKGRLIVNFDSLGIGLQIE
                     INVNQLEPSSV"
     gene            complement(2280..2816)
                     /locus_tag="PLG24_10815"
     CDS             complement(2280..2816)
                     /locus_tag="PLG24_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HMZ25427.1"
                     /translation="MIETLISSKTRVKLLFKFFLNKETTAYLRGLENEMGESTNAIRI
                     ELNKFEKAGLLKAETSGNKKIFSANTSHPLFREIHNILLKHIGIDQIINNVVERLGNV
                     DRVYLAGSFAKGIDGPIIDLILIGNVERDYLSQLITKAENLIKRKIRYVIFNESEYTD
                     YLTQSKDLEPLLLWSDKS"
     gene            complement(2917..3555)
                     /locus_tag="PLG24_10820"
     CDS             complement(2917..3555)
                     /locus_tag="PLG24_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25428.1"
                     /translation="MCRVTKLLFLILILASNLTFGQSGYERAMTRGIQMIKNAKVPED
                     MNRAEGYFEQIAGTEKNQWLPYYYAAFTKILTAYLYESSNKDYIAHKAESMIFKADSL
                     QPDNSEIYCLKHMVASLAFQVDPGNRWLSYGVEAGAALDKAKRLDPSNPRPYVLEGLQ
                     LMNAPGEYGGSKEKAKVAFKKALDLFENFKPISDIAPSWGLEQAKTALEGLD"
     gene            complement(3645..>3852)
                     /locus_tag="PLG24_10825"
     CDS             complement(3645..>3852)
                     /locus_tag="PLG24_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HMZ25429.1"
                     /translation="FSLVTLPVEFDASNRALAWLNNSGLTYGEEYKGAKDALWWAAMT
                     YVAAALSALAMLIYLVLRYMGNRE"
BASE COUNT         1079 a          866 c          757 g         1150 t
ORIGIN      
        1 tacggtgata atgtaaaaat cgaaaccttt gattttttgt gcatcatctg taaaagacaa
       61 tccgatttga tcattctggt taagtacgga gatcaggttt gattcttcca cttccagggt
      121 atggtcttta ccctttctca gttcttccaa tcgattttta ttgatgtcaa aaccaattac
      181 atgatagtgt ttggccagtt ccacagccag aggcaggccg acataaccga ggccgataac
      241 ggctattttc gtattcaagt tatatagtat ttaaattaaa aaatgctaat tatttcgggg
      301 tgagcccggg tcattacacg gggcggccga tactttcata attaaatcct ttttcctgca
      361 tttctttcgg gtcaaaaata ttgcgcccgt caaaaatcac cttatccttt atgctatgaa
      421 acatggagtc aaattgcggg gagcggaatt cattccattc tgtcataatc atcagggcat
      481 cgcaatcact cagtacttca tactggtcga cagcataaaa caatttttct ccatagatcc
      541 tcctaacatt gtccatggct tccgggtcaa aaacgcggat tttggcacct gcatccagca
      601 atttatcaat gatgtacaga gcgggagctt cgcggatatc gtcggtattt ggtttgaaag
      661 aaagtcccca gatggcaatg gttttaccgt tgaggttacc tttatagaag ttgtgtaatt
      721 tgtcgaacag gatggttcgc tgatttatat ttacctgaat cactgcatcc agaatccgga
      781 aattaaaatc gttctgatct gctatcttat gaagggcctt tacgtccttg ggaaagcagg
      841 atcctccata gccgatgccc gggaaaagaa accgcttacc tatccgcgag tctgcaccca
      901 tacccaggcg aaccatatcg acattggcgc ccacacgttc gcataggttt gctatttcat
      961 tcataaaggt gattttagtt gccaggaatg aattggcggc gtatttggtc atctcggcag
     1021 agcgttcgtc catgaagtaa atggggttac cctgtttgac aaagggctcg tagagtcggt
     1081 ggatgatctc gcgtgcacgt ggggatgagg taccgatgac gactctgtca ggcttcataa
     1141 agtcttcaac ggcaacacct tcgcgtagaa attccggatt ggaaaccaca tcgaaaagct
     1201 ccgcggctgc attctgagca attgcctgat gtaccaggtc ggctgtacca acgggaaccg
     1261 tacttttatc aacgatgatt ttataggaag aaatcatttt acccagctgg tttgccacat
     1321 tcaagacata ggaaaggtcg gccgaaccgt cttctccttc cggtgtaggc agggcgagga
     1381 agatgatgtc agcgaagtca accgcctttt tcaggtcagt agtaaactca agacgttttt
     1441 tctctaaatt tctttgaaaa atgacttcca gtcccggctc atatatggta ataacgcctg
     1501 atttaaggct atttacctta ttttcatcaa tatcgacaca catcacggaa ttacctgtct
     1561 ccgaaaagca ggtgcctgtc actaaaccaa cgtatcctgt tccaattacg gaaatattca
     1621 tgctaaaaat tcatttacat ttaaaaaatt aaacatggct tcgccgtcaa taagtcgcac
     1681 ttattgtatc caaaatttat atacatttat ctgtataata attatttata tattataatt
     1741 tgaatatatt tttcaataat tatacagagg atggttcgag ttgattcaca ttgatttcta
     1801 tttgcaggcc tatacccaaa ctgtcaaagt tgacgatgag tctgcctttt gatttaactt
     1861 cactgacaat gccctgcatg ccgaataaag caccattatt gacgtggact ctttgtccgg
     1921 tctgatatct tacttcctta aatgcgtact tttcatccag accttcgatg cgatgcagga
     1981 tatccatttc cgcttgagtc acaggcgaca ataattttcc taccttcaca aagccaaata
     2041 cgtgttcagt ctccaacaca gaaacgtatt gtttccgatc gatatggacg aaaacatagg
     2101 tggataaaac aggcaccatc cggtgcacaa tcttcctgag gtatctgctt gtgtatctgc
     2161 gaaaaggagt gtatgcttca atgtccttgc gctccaattg tgcaatgaca accttttcac
     2221 tcttatattt gacctttacg gcataccatt gttttccaac ccaagtattc atcatgctct
     2281 caacttttat cggaccaaag cagcaacggc tccaggtctt tactttgggt taaataatcc
     2341 gtatattccg attcattgaa gattacatag cgaatttttc tcttaatcag gttttcagct
     2401 ttggtaatca attgagacag gtaatctctt tctacattgc ctatcaaaat aaggtcgatg
     2461 atcggcccgt caataccttt agcaaatgaa cctgccagat acaccctgtc aacattacca
     2521 aggcgttcta ccacattatt gatgatctgg tcgatgccga tatgtttaag gagaatgttg
     2581 tgaatttccc gaaaaagggg atgtgatgtg ttggctgaaa agattttctt attgccactt
     2641 gtttcggctt tcaggagacc ggccttttca aacttattca gttcaatccg gatggcatta
     2701 gtagattcgc ccatttcatt ttccaaacca cgcaggtagg cagttgtttc cttgttgagg
     2761 aaaaacttaa agagtagctt gactcttgtt ttcgatgaaa ttaaggtctc tatcataaaa
     2821 attgagtagc aaaattactc aattttataa aactgaccaa tcatttattc taaaaaatac
     2881 cggaaaaaaa gatcatctca atattccctt caacaatcaa tccaagccct ccaaagccgt
     2941 tttcgcctgt tcaagtcccc aggaaggagc gatgtcagaa attggtttga aattttcgaa
     3001 caggtccaac gcttttttaa aggcaacctt agctttttcc ttactgccgc catactcacc
     3061 gggagcattc atcaactgca ggccttccaa cacataaggt cgcggattgg aaggatccag
     3121 gcgctttgcc ttatcaaggg ctgcgccggc ctccacccca taggaaagcc atcggttgcc
     3181 cggatccacc tgaaaggcga gggaggcaac catatgtttc agacagtaaa tttcagaatt
     3241 gtcaggctgc aaagaatctg ccttgaagat catggattct gccttatgag caatataatc
     3301 tttattggag ctttcgtaca ggtaggcggt cagtattttg gtgaaagcag cataataata
     3361 gggaagccac tgattcttct ctgttccggc aatttgttca aaatagcctt ctgccctatt
     3421 catatcctca ggcaccttgg catttttaat catttgaatg cctcttgtca ttgcccgttc
     3481 atatcctgac tgaccaaaag tgaggttgga tgccagaatc aatatcaaaa aaagcaactt
     3541 tgtcacccta cacataagag cttattgttc cattataata atgtctccta tcgggaagtc
     3601 cggatagatc atactttact gtatttcaac aagggaatgc aaccttattc ccggttgccc
     3661 atatacctca ataccagata gatcaacata gccaaagccg aaagtgctgc tgcgacatag
     3721 gtcattgctg cccaccaaag tgcatccttt gcgcctttat actcttcacc ataagtcaag
     3781 ccggaattat tgagccatgc caaagccctg ttactcgcat caaactcgac cggaagagtc
     3841 acaaggctaa at
//
LOCUS       DAOQQN010000316         3843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_11493_length_3843_cov_5.731785, whole genome shotgun sequence.
ACCESSION   DAOQQN010000316 DAOQQN010000000
VERSION     DAOQQN010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11493_length_3843_cov_5.731785"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..1477
                     /locus_tag="PLG24_10830"
     CDS             32..1477
                     /locus_tag="PLG24_10830"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HMZ25430.1"
                     /translation="MFNEFITLDKADQQVLISPPLTMRPKISVKDKSVLFRFDEKEEL
                     RPNTTYIINFGSSIKDYTEGNIVHDFQYVFSTGDNIDSLQLSANIKDAETGKPADNTV
                     LMLYRDLSDSVVYKILPDYAGRTDKDGSVTLRYMQPGLYQAVALNDENFNYKYDLTKE
                     KAGFLRDTILLPHHGKPLDMQIFGRDQPVRVTAVDSASNQRIRFIFEPSTAGIKLYSL
                     MEEPNNKIITGLKYIDVYYGDTTRSINAILMRPGFEADTIRHKLSGKVAARKPKVSQE
                     SADIKAPPHVPGEPVRIRFNVLLAAVDTSTMTLMTLKSKQAVPYLATIDPSDNNYMLL
                     LCNWMTDSTYLLNLQQGAIRDVLGNVNDSLEIQFKITDPSTLSALNIRIDSLQQDFDY
                     IIRLAKGNDIIRQWTISGKDSFKAEVLNLPAEAYDIHIMEDINKNGRIDKGNFDKKED
                     PEKIYTRKLEQLRQNWTVDIEVNMNDFKNIR"
     gene            1872..2195
                     /locus_tag="PLG24_10835"
     CDS             1872..2195
                     /locus_tag="PLG24_10835"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HMZ25431.1"
                     /translation="MSSYFENVYALVRLIPRGRVTTFGAIANALGLGSARMVGWALNQ
                     LSGSDSSVPAHRVVNRKGMLSGQLHFDPGYSMAQRLIDEGVQVENDIVQDFKELFWDP
                     ALLAE"
     gene            complement(2333..2719)
                     /locus_tag="PLG24_10840"
     CDS             complement(2333..2719)
                     /locus_tag="PLG24_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25432.1"
                     /translation="MMMPATLGIQKAIFALLCLSFLLPACNKSNDIGDGNPPSGCLIT
                     LYEKDRFKGNAIILQGPANYLDLKAIPGSSKNWEKVAQSFEATGKAVVVFWKEPDLRG
                     DSISYPAASMISQLPFAPGSLKIKCD"
     gene            3070..3234
                     /locus_tag="PLG24_10845"
     CDS             3070..3234
                     /locus_tag="PLG24_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25433.1"
                     /translation="MKWFVLIAVIYIVFRVYRIGLINGSSSEKEKINRPGKSEAKDSN
                     IEYSDYEEIK"
     gene            3234..>3843
                     /locus_tag="PLG24_10850"
     CDS             3234..>3843
                     /locus_tag="PLG24_10850"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HMZ25434.1"
                     /translation="MQFSEQVLAKNHQYCIINKPAGQPVQPDKTGDISTMDMCKKYFH
                     HDVYLVHRIDRPVSGLVLFAKNTRSAAWLSELFRSKELDKTYLAIVENEPPHTSGSLV
                     SRIIEKKTGGNKSRIGEDNSANKGREAITKYEVIGRTDKYTLLAIKPVTGRKHQIRAQ
                     LAEAGCPIQGDVKYGARRGNKDRSIGLHAWRLSFTHPITGEIA"
BASE COUNT         1197 a          816 c          826 g         1004 t
ORIGIN      
        1 gttcaatttt cttatttaca attgaaatat tattttcaat gaatttataa cattggacaa
       61 agctgaccag caggtactga tcagccctcc gttgacgatg agaccaaaaa tctctgtcaa
      121 ggacaaatcc gtattgtttc gatttgatga aaaggaagag ttgcgtccca acaccacgta
      181 tatcatcaac tttggcagtt ccattaagga ttataccgag ggaaatattg ttcatgattt
      241 ccaatatgtg ttcagcaccg gtgacaacat tgactcctta cagctttcgg cgaatatcaa
      301 ggatgcagaa acaggcaagc ctgccgacaa tacagtgctg atgctgtata gagacctgtc
      361 ggacagtgtg gtgtataaga tattaccgga ttatgccggg cgcacagata aagatggaag
      421 tgtgaccctg cgatatatgc agccgggcct ataccaggcg gtagccctca atgatgaaaa
      481 ttttaattac aaatacgatc tgaccaagga aaaagccgga tttttacgag atactatcct
      541 gttgccccat catggcaaac ccttggacat gcaaatattc gggagagatc aacctgtccg
      601 ggttactgct gtagatagtg catccaacca gcgtatccga tttatcttcg agccatcaac
      661 agccggcatc aagctgtatt cactcatgga agagccaaac aataagatca taaccggcct
      721 caagtatatc gatgtatatt atggagatac cacccgctca atcaatgcta tattaatgcg
      781 ccccggattt gaagcggaca ccatcaggca taagctttca ggtaaggtgg ctgcacgaaa
      841 acctaaagtc tctcaggaat ccgccgatat aaaagcacca ccgcatgtgc ccggagaacc
      901 tgtcagaatt cgttttaatg tcttgttggc tgctgtcgat acatccacaa tgactttgat
      961 gactttaaaa agtaagcaag cagtgcctta ccttgcaacc atcgatccgt ctgacaacaa
     1021 ttacatgttg ctcctttgca attggatgac cgactccact tatcttttga accttcagca
     1081 aggcgctatc cgggatgtcc tgggcaatgt caatgactcc ttggagattc aattcaaaat
     1141 aaccgatcca tctaccctta gcgcccttaa tatcagaata gacagcctgc aacaggactt
     1201 tgattatatc atccggctgg ccaaaggaaa tgacatcatc agacaatgga caatttcggg
     1261 caaagattcc tttaaggcag aagtattaaa tctcccggca gaagcctatg atatccacat
     1321 catggaggac atcaataaaa acggaagaat cgacaagggt aattttgaca aaaaggaaga
     1381 tccggagaaa atttatacca gaaagctgga gcaactacgt caaaactgga ccgttgatat
     1441 cgaagtcaac atgaatgatt tcaaaaacat tcgataggaa gcaagcgata ttgttgaaag
     1501 tcagtcgatc aggacaacaa aatagccgcc agattggctg aaagagattg gttatgtaag
     1561 ggtaaatcga agtaggtagg aaaataatag tgtccgagtg agagggcaca aggaatttaa
     1621 aagatgataa tctaaattgc tgcttaaatc caagtcaatt ttttaatagc ctcccttctc
     1681 aagcttgcca gatcaaataa gtcggttatt atttatctac ctaattccat ttttttcggg
     1741 cccaacttca cacccgaaat gaaaaacaac agaagctaat gctataatgg gaaaagtcag
     1801 agcatattat gtatcctctg atgaccaaaa tttacagaac tttattcgat gatcccactt
     1861 atttttgtgc catgtcttca tattttgaaa acgtgtatgc tttggtaagg ctaataccac
     1921 gaggcagagt gactacattc ggtgccattg ccaatgcgtt aggactcggt tctgccagga
     1981 tggtgggctg ggcgctcaat caattatccg gaagcgactc atcggttccg gcacatcgtg
     2041 tagtcaaccg caaaggcatg ttaagcggtc agctgcactt cgaccccggg tattctatgg
     2101 ctcaaaggct catcgatgaa ggggtgcagg tagaaaatga catcgtgcag gattttaagg
     2161 aattgttctg ggatcccgcc ctattggcgg aataaatgaa tgcatggttt taccatcact
     2221 cttgcattgc ttcgaaaggc gataagtgct atccttcaaa ccatgaattt ccaattggtt
     2281 tttccaggga ccaataagtc atcaactaaa ttaccaaagg aaaattaatt ggctaatcgc
     2341 acttaatctt cagacttccc ggggcaaagg gcagttgaga tatcatcgat gctgccggat
     2401 aggaaatgga gtctcccctt aggtcgggct ccttccagaa tacaactact gcctttccgg
     2461 ttgcctcaaa ggattgggct accttttccc agttttttga gcttccggga atagccttca
     2521 ggtccaggta atttgccgga ccctgaagaa tgatggcatt gcccttaaat ctatctttct
     2581 catacaatgt gatcaggcaa cctgatggcg gattgccatc ccctatatcg ttggacttat
     2641 tacaggcagg aagcaggaag gacaggcaca acagggcaaa aatggctttt tgaattccta
     2701 aggtggcagg catcatcata aatcactcat ttaaattgtg ttgcaaacat agtcaaaaag
     2761 tgaaaaatga acattagttt gagaaaaact ctcctttaac aattagcatt acaattgctg
     2821 ctcgggcaga ttatggtata tggaattcat gttataccaa gatgatttat atattagtcc
     2881 ataagttttt ggtataatgc agaaggtaca ttcagtattt tagtcggccg attttcgtac
     2941 aatcatgcag aaagcgcatc aaagccaaca atccctataa tccgtaatat tcaaagaaca
     3001 aggctcactg taagtttaat ataaaagttg gccgattttt tgtaattttg cagtcttaat
     3061 cattatgctg tgaagtggtt tgtcttgatt gccgttatct acattgtttt ccgcgtttat
     3121 cgcattggtt tgatcaatgg ttcctcaagt gaaaaggaga aaatcaaccg acccggcaaa
     3181 tcagaagcta aggacagtaa tattgaatat tcggactacg aagaaataaa ataatgcaat
     3241 tcagcgaaca ggtactggcc aaaaatcacc agtactgcat cattaataag cctgcaggac
     3301 aaccagtaca gcctgacaaa actggtgaca tcagtacgat ggacatgtgc aaaaaatact
     3361 ttcaccacga cgtatatctt gtccaccgta tcgatcgacc tgtttcagga ttggttttat
     3421 ttgccaaaaa taccaggtcg gcagcgtggc taagcgagct cttccgttcc aaagagttgg
     3481 acaagaccta tcttgccatc gtggagaatg aaccgcctca tacatcagga agtcttgtaa
     3541 gccgtatcat tgaaaaaaaa accgggggta ataaatcaag aatcggcgaa gataattcag
     3601 ccaataaggg gcgtgaggca attacaaaat atgaagtaat aggacgaacg gacaaataca
     3661 ccctcttggc cataaagcct gtgacaggac gaaaacacca gatacgggca caactcgctg
     3721 aggccggttg cccaatccag ggagatgtca agtatggagc acgacgaggc aataaggatc
     3781 ggagtattgg gctccatgca tggaggcttt ccttcaccca tccgatcaca ggagaaattg
     3841 cta
//
LOCUS       DAOQQN010000317         3840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_11502_length_3840_cov_6.414267, whole genome shotgun sequence.
ACCESSION   DAOQQN010000317 DAOQQN010000000
VERSION     DAOQQN010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11502_length_3840_cov_6.414267"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            707..3478
                     /locus_tag="PLG24_10855"
     CDS             707..3478
                     /locus_tag="PLG24_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ25435.1"
                     /translation="MKFYFLFFLVLTLTLTNAQPSTWSSSGLGGGGSLFAPSISPVNS
                     SIMYMQCDMSEVFHTSDAGIHWQPIHFKQLISTGGTHPVVFTNDPSILYTVNYGFLDD
                     TRFPVKSVDGGNTWNQLASDPTGGDVWFISSDPNSTQRIIISSYTAMYLSVDGGNTFS
                     LKYNHGSDFLISGVFWDGNDIYIGTQIGLIVSHDGGVSFALDSSPGIPAGYGFMSFTG
                     SKSNGITRLMGTIAAQDDLYPGVNALDIDIYSSIIRIDYGTTGWTNAVGGINPTHNLF
                     YIASALNNSSVFYVGGTNPNTSYPVIYKTSDGGNSWAEVFLTVNNQNISTGYSGYQGD
                     EDWWYGEIVFGLAVAPNDPQTVIYTDFGFAHISTDGGTNWRQSYVASEDQNPAGSPTA
                     KGKAYGNNGLENTSCWNMHWCSANEDIILASYTDITATKSIDGGIKWSFDFNGINYNT
                     VYHIIEHPTNGTLYAATSSVHDIYQSTYLTDSRIDGGTGAILYSTDQGLHWNLLHNFN
                     HPVIWLAIDPKNNNRMYASVVHSTAGGIYLTSDLNNGSLSNWSSTSKPARTEGHPYNV
                     FVLNDGTVVTTWSGRRTSGGFTASSGVFASSDQGATWTDLSDRPNMDFWTKDITIDPN
                     DANQNTWYVSVFSGWGGPSNGKGGLYKTSDRGVNWTKIFNGYRVESCTVDPVNASKIF
                     VTTENEGLWYSSNANSASPSFTQQDSYPFMHPIRVLYNPNDFTEIWVTSFGNGIKVGT
                     APSIPLAIHNLQLDGRGRVDGNSINWSFDEITEPLARLGLQRSEDGYRFEEIYRESRP
                     KITSGQFFDRFPSVTSYYRIEFYFMSGKSGYSNILTINNEHIDPIQVYPNITEDLVHL
                     EFPGNLNNLYQINVVNAAGIPVKAFSHSNYLSGNKATVSLRDLPGGFYIIRIMSETGF
                     QYSVKIFRK"
BASE COUNT         1077 a          764 c          754 g         1245 t
ORIGIN      
        1 tgaattcgca gtgaaggtaa ggaaaagatt tgggatgtgc aaggggtggg tggggaaaaa
       61 ttgaagtagg taggaaagta ataattcaaa aaacgctata cacaaggatt tggaggcaaa
      121 tgaaaattta attcctgaat aaaatatctg aattttggca aggctgcccc gaaaggcagt
      181 aaagtcaagc attgtggcta ttaatcatct acctacttcg gaggcggcac cacccacaac
      241 ttccccaaaa atcgtagtag gtaggaaagt aataatagat attccccact gaacaattac
      301 ttttgtcaca attgaattat ttatttctct gatttgttac tttaacttcg tctacatctc
      361 tacaatcatc catccttgca tactttgcag ttattaatta tctacacact tccactgctc
      421 tcaactccca gccctaccag ccttttaaat gctattcggc atacttagaa cttaccattt
      481 tttgtttatc actgtttttt agtatttttt aaaattttat atatatttca cttctcaaaa
      541 atcttttttt gtatttttga gggcatctcc tgatgatcct tttttttgtc atgactgttt
      601 cttgataatc cggaattcaa tccagattcg tgttttttaa agatttctgt tgtgtcattt
      661 tttttaattc ttatccattc ttactaacac tcaaattcat tttgccatga aattttactt
      721 cctcttcttc ctcgttttaa ctttgacctt gactaatgcc cagccatcta cctggtcttc
      781 atccggcctg ggcggtggag gttctctttt tgcaccatcc attagtcctg tcaactcctc
      841 tattatgtat atgcaatgtg atatgtcaga agtttttcat acttcggatg caggtatcca
      901 ttggcaacca attcacttca aacaattgat ttctactggc ggtactcacc ctgttgtttt
      961 caccaatgat ccttccattc tttatacagt caattatggc ttcctggatg atactagatt
     1021 tcccgtcaaa tcagttgatg gaggcaatac atggaatcaa ctggcttcag atcctaccgg
     1081 tggtgatgtt tggtttattt cttcagaccc taattctaca cagcgtatta tcatctcatc
     1141 ctataccgcc atgtatcttt cagttgatgg aggtaatacc ttcagcctta agtacaatca
     1201 tggctccgac tttcttattt ctggagtttt ctgggacggc aacgatattt atataggcac
     1261 acagatcgga ttgattgttt ctcatgatgg aggcgtcagc tttgccctgg attcaagccc
     1321 cggcataccc gcaggatatg gctttatgag ctttacaggt tcaaaaagca atggtattac
     1381 gaggcttatg ggcaccatcg ccgcacaaga cgatctttat cctggtgtta atgccttgga
     1441 tattgatata tattcctcca taatcagaat agactatgga acaaccggct ggaccaatgc
     1501 agtcggtggc atcaatccga ctcacaattt gttttatatt gcctcagcat taaataattc
     1561 ctctgtattt tatgttggtg gcaccaatcc gaatacctcc tatccggtga tatataagac
     1621 ttctgatggt ggtaattcat gggccgaagt ttttttgaca gtcaacaatc aaaatatttc
     1681 aactggatat tccggttacc agggtgatga agactggtgg tatggagaga ttgtcttcgg
     1741 cttggctgtg gcacctaatg atcctcagac tgtaatttat actgactttg gctttgctca
     1801 tatttcgaca gatggaggca ctaattggcg acagtcgtat gtcgcttccg aagatcaaaa
     1861 tcctgccgga agtcccactg caaagggtaa ggcctatggt aataatggtc ttgaaaatac
     1921 ttcctgctgg aatatgcatt ggtgctctgc caatgaagat ataatcctgg cttcctatac
     1981 tgatataact gcaaccaaaa gcattgatgg tggcataaaa tggtcttttg attttaatgg
     2041 aatcaattat aataccgtat accatatcat tgaacacccc actaatggta ccctatacgc
     2101 tgcgacgagc agtgtccatg atatctacca aagtacctat ctgactgaca gcagaataga
     2161 cggcggtacc ggggctatcc tatatagtac cgaccagggt cttcattgga atttgttgca
     2221 taactttaat catcctgtaa tctggctggc gattgaccct aaaaataaca acaggatgta
     2281 cgccagtgtt gtccatagta ctgccggcgg aatttattta acttcagacc ttaacaatgg
     2341 atccttatca aactggtcat ctacttcgaa gccggcacga acagaaggtc acccatacaa
     2401 tgtttttgtt ttgaatgatg gtacggtggt aacaacctgg tcaggccgaa gaacttccgg
     2461 gggatttaca gcaagctccg gagttttcgc tagttcagac cagggcgcca cctggacgga
     2521 tctttcggat agaccaaata tggatttttg gacgaaagat attacgatag atcctaacga
     2581 tgcgaaccaa aatacttggt atgtttcggt ttttagtggc tggggcggtc cttccaacgg
     2641 taaggggggg ctgtataaaa catctgatcg tggtgtcaac tggactaaaa ttttcaacgg
     2701 atatcgggtg gaatcctgta cggtcgatcc ggtcaatgcc tccaagattt ttgttactac
     2761 tgaaaatgaa ggtttatggt atagctctaa tgccaactca gcttcaccct cttttacgca
     2821 acaagattct taccctttta tgcatcctat tcgcgtttta tacaatccga atgactttac
     2881 tgaaatttgg gtcactagtt ttggaaatgg cattaaagtt gggaccgctc cctctatccc
     2941 attagcaatc cacaatttgc agttagatgg aagaggtagg gtagatggaa attctataaa
     3001 ttggagtttt gacgaaatta ctgaaccttt agcaagactc ggcttacaga gaagtgaaga
     3061 tggatacagg tttgaagaaa tttatcggga atcaagacct aaaattacat caggtcaatt
     3121 tttcgaccgg tttccatcgg tcacttctta ttacagaatc gaattctatt tcatgtccgg
     3181 caaatcaggt tactcaaata ttttgactat caataatgaa catattgatc cgattcaggt
     3241 ctatcctaat attacagagg atctggttca tttagaattc ccagggaatt tgaataactt
     3301 gtaccagata aatgtggtga atgcagcagg cattccggtt aaggcttttt cacatagcaa
     3361 ctatttatcc ggcaataaag ctacggtatc tctgcgtgat ttgcccggag gcttttatat
     3421 cataaggatt atgtctgaaa caggttttca atattctgtt aaaattttca gaaagtagga
     3481 tgaattgttg gaatctttat taatcaagat tatttcctga atttttatcc tgcaggaaaa
     3541 cattttggct cgaatgcctt ttatgttttt ttttgtcaca tttcaaatga aagggggcga
     3601 gatcaagttg gatttttatg tgaaatagaa agggaaatga taactgaggt acggctgaag
     3661 tagattaaga ttcttctaaa aaattcattc ccattttcac accccattag cgtatttgct
     3721 aataaatttt gaaataattt agaccggaat cggcgaattt tacgcagaaa ataactattc
     3781 tcaggtaata ccgaaggtac attcgttata gaccaatttc attggtctat acgactgtaa
//
LOCUS       DAOQQN010000318         3838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_11508_length_3838_cov_6.307164, whole genome shotgun sequence.
ACCESSION   DAOQQN010000318 DAOQQN010000000
VERSION     DAOQQN010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11508_length_3838_cov_6.307164"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PLG24_10860"
     CDS             <1..851
                     /locus_tag="PLG24_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25436.1"
                     /translation="GAYDATGQDKTLIYPVGINYIEPHSFRSSILISQGEPLRLIDYI
                     PLYKENPHKAIKLLTDDLEFQLKKEVLHIEEKEAEKLAQTGFEILDNAYPGTLFSLAK
                     NSQLFNRLKMWSIQVNKEAKDPDSSWLVDLKELKDILISSRIKTKWPFFNKSFPLNML
                     LFILLFPIGILAQGFFYPPILLSKWLTIRFKPRREFFLSIRIGISWAILTSWISIFFI
                     IIGVEWSWRYGAAFVFLSPIIAKIGLIWWNARNILIQKIRWSSLDDIEEERASVILEK
                     LNLNRD"
     gene            925..1965
                     /locus_tag="PLG24_10865"
     CDS             925..1965
                     /locus_tag="PLG24_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMZ25437.1"
                     /translation="MKVHLISGGCGFVGRNMVKRLYRTTQDKIFFIDDLSVGTDPSTW
                     LDHALVGEKNGIKVYGQDERLYFLKADFRDVVHALSVDPEYIKKTYGLDFVDFGDIYH
                     YAAIVGGRAKIDGDPMMVALDLSIDAEFFYYITRHKPDRVLYPSSSAAYPINLQTETG
                     AIALAEHNIDFNNMGQPDMTYGWSKLTGEYLAKIAAQYYGVHITCIRPFSGYGEDQDL
                     TYPIPSLAKRAALKENPFEVWGTGYQGRDFVHIDDVIDCIEVAMNHISDGTAINIGMG
                     KLTSFRDIIELMTGFAGYNPEIKQLLDKPVGVHSRYCDMSFVEKTLGWKAKISVEEGM
                     RRVYEATIQKFS"
     gene            1971..3470
                     /locus_tag="PLG24_10870"
     CDS             1971..3470
                     /locus_tag="PLG24_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25438.1"
                     /translation="MSLKKIIRPSLAFLVVIYMATTGCFKDEIVNTDSNVKLRFSVDT
                     LLFDTTFTQVGSATKIVKIHNDGNRPIEISKIKIRGTQGLHFTLNVDGINGNEITNAR
                     IESKDSIYVFCKVNVDPDQSVSVSPFVLTDYLDCTINGNLQSIVLVAWGQNANYITGK
                     DNQGKQSLLTCNFGTISWNDPKPYVIYGSLVIDQCKLIIPAGTRIYVHGGLVFPDKAS
                     PFIDGRIFIAEDGQLLIDGTKEKPVLFRGDRLEKEYNNQSGQWGGIILYKSKFVNVFN
                     YLDLRNSNFGVVADSATALHINHSIIDNVSLSCLIGNHATIKATNCLFANSAGDNILL
                     TYGGNYSFDYCTVPNSTGQTSALSMDNYTCTLNELGICKEGTIRANKAELKVRNSILS
                     GVDNDEIKLVDVFEGKQQDMFQFNISNSVIRVKENLQNDYFNERIKDVYILQNLDRLY
                     ANENKSDYRLDSISVAKYLAMPIPTIPDDLPGNIRKTDKSDAGCYEYLD"
     gene            complement(3631..>3838)
                     /locus_tag="PLG24_10875"
     CDS             complement(3631..>3838)
                     /locus_tag="PLG24_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25439.1"
                     /translation="PDKALAHRVVTNMINWLYARHGHVTYLQDYLNEYCYRYSRHRMK
                     GEILDDIISRMVRHRPMSEKRFIS"
BASE COUNT         1238 a          682 c          773 g         1145 t
ORIGIN      
        1 tcggtgctta tgatgcgacc ggccaggata aaaccctgat ctatcctgtt ggtattaatt
       61 atattgaacc tcacagtttc cgttcaagca ttctgatctc acaaggcgag cctctaaggt
      121 tgattgatta tataccccta tataaagaaa atccacacaa ggcaataaag ttactcactg
      181 atgaccttga gttccaatta aaaaaggaag tcctgcatat tgaagagaaa gaggctgaaa
      241 agctggcaca gaccggattt gaaattttgg acaacgctta tcccggcacg ctatttagcc
      301 tggcaaaaaa cagccaattg ttcaataggt tgaaaatgtg gtcgatacaa gtgaataaag
      361 aagccaagga ccctgattct tcctggttag tcgatcttaa agaactcaag gatattctga
      421 ttagttccag aatcaaaaca aaatggccat tcttcaataa atcattccca ctcaatatgt
      481 tactcttcat cctattattc cctatcggca tattagctca aggttttttc tatcctccga
      541 tattattatc aaaatggctt acaatcagat tcaaaccaag acgtgaattt tttctgtcta
      601 tccgtatagg tatttcctgg gctattttga catcatggat ttccatattc tttattatca
      661 ttggtgtaga atggagttgg cgatatggag cggcctttgt gttcttatct ccgataatag
      721 caaaaatcgg tttgatttgg tggaatgcga gaaacattct gattcaaaaa atcagatggt
      781 ctagtctcga tgatattgaa gaagaaagag catcagttat tctggaaaaa ctaaatctta
      841 atcgagatta ataaatttaa attataaaac tttgcaataa taattcgaac aattaaaaca
      901 tttaaataag aagaaacaat ttttatgaag gtacatttaa tctccggagg gtgcggtttt
      961 gtaggacgca atatggtcaa aaggctctat agaactacac aagataaaat attctttatc
     1021 gacgatctca gtgtaggtac cgatccttcg acctggcttg atcatgcctt ggttggcgaa
     1081 aaaaatggaa ttaaagtata tggccaggat gagcgattgt actttctaaa agctgatttt
     1141 agggatgtag tacatgcttt atctgttgat cctgaatata ttaaaaaaac ctatgggtta
     1201 gattttgtgg attttggaga catctaccat tacgcagcaa ttgtcggagg aagagccaaa
     1261 attgacggtg atccgatgat ggttgccctt gacttatcga tagatgctga atttttctat
     1321 tacataacac gacacaagcc tgatcgtgtc ctttatccga gtagtagtgc cgcatatcca
     1381 atcaaccttc aaactgaaac aggagctatt gctttggcag aacataatat tgacttcaat
     1441 aatatgggac agccggacat gacttacgga tggtcgaaac tgaccggaga atatctcgca
     1501 aaaattgctg ctcagtatta tggtgtccac atcacctgta tccgcccctt ttccgggtat
     1561 ggagaagatc aggacttaac ctaccccatt ccaagtcttg ccaagagagc tgcactgaag
     1621 gaaaatcctt tcgaagtgtg gggaacgggt tatcaggggc gtgattttgt acatatagat
     1681 gatgtcattg actgtattga ggtagcaatg aatcatatca gcgacggaac tgccattaat
     1741 ataggtatgg gaaaattaac ctcatttcgg gatatcattg agctaatgac gggatttgcg
     1801 ggctataatc ctgaaattaa acaactgctg gataagccgg ttggtgtaca tagcaggtat
     1861 tgcgatatgt cgtttgtaga aaaaactctt ggatggaaag ctaaaatttc agttgaggaa
     1921 ggaatgcgac gcgtatatga ggcaaccatt caaaaattca gttgaacatc atgtcattaa
     1981 aaaaaatcat acgaccaagc ctagcctttc tggttgttat ttacatggca actaccggtt
     2041 gctttaaaga tgaaatagtc aatacggact ccaatgtaaa attgaggttt tctgttgaca
     2101 cgctattatt cgatacaact tttacccaag ttggttccgc tactaagata gttaaaatac
     2161 ataatgatgg taataggccg attgaaatta gtaagattaa aattagagga acacaaggat
     2221 tgcattttac cttgaatgta gatggaataa atggaaatga aatcaccaat gcaagaatag
     2281 aaagtaaaga tagtatctat gttttttgta aggtaaatgt cgatcccgac cagtcagtgt
     2341 cagtttcacc ttttgtattg accgattacc tggattgtac aatcaacggc aatttgcaat
     2401 ctatagtact tgttgcttgg ggacagaatg caaattatat cacaggtaaa gacaatcaag
     2461 gcaaacaaag tcttttgaca tgcaattttg gtacaataag ctggaacgat ccaaaaccat
     2521 atgtaattta tggttcatta gtaatcgatc agtgtaaact gattattcca gcaggtaccc
     2581 gcatttatgt acatggaggc ctggtatttc cggacaaagc cagtcctttt attgacggaa
     2641 gaattttcat agccgaggat ggccaacttt tgatagatgg tacaaaggaa aaacctgtat
     2701 tgttcagagg ggaccgttta gaaaaagagt ataacaatca atctggacaa tgggggggaa
     2761 ttatacttta caagtccaag ttcgtcaatg tattcaacta cctggattta cgcaattcca
     2821 attttggggt agttgctgac tcagcaaccg ccctccatat aaatcatagt atcatagata
     2881 atgtttcact tagttgcctt attggtaatc atgccactat aaaagctacg aattgccttt
     2941 ttgcaaattc cgcgggtgat aatatattat taacttatgg tggaaattat agcttcgatt
     3001 actgtaccgt accaaattcc accgggcaaa cctcagcgct ttcaatggac aattacacct
     3061 gtacactcaa tgagttaggt atttgcaaag aaggaaccat tcgggcgaat aaagcagaat
     3121 tgaaggtgag aaatagtatt ctatccgggg ttgataacga tgaaataaaa ttggttgacg
     3181 tatttgaagg gaaacaacag gatatgttcc agtttaatat ttcaaattcg gttatacgtg
     3241 taaaggaaaa cttacaaaat gattatttca atgaacgaat aaaggacgta tacatattac
     3301 agaaccttga caggctatat gccaatgaga ataaatccga ttataggctg gattcaatat
     3361 ctgtagctaa gtatcttgcc atgcctattc cgacaattcc tgatgatttg ccgggtaata
     3421 tcagaaaaac tgataaatca gacgcaggat gttatgagta tttagattaa taaagtaagt
     3481 tactaaattc aattgtgtaa tttctaacca aattgagtcc aaaaggctat gtgttttaat
     3541 tacatataag atcacaaatt tgacggccat tactttatta aatcactttg taatataact
     3601 gctcatacag aatcataata gtccaaaaga tcaactaatg aacctttttt cactcatcgg
     3661 tctatgcctg accattcttg agatgatatc atccaggatc tcgcctttca tgcggtgcct
     3721 gctgtagcgg taacagtatt cattgagata gtcctgcaga taggtcacat ggccatgtct
     3781 ggcatatagc cagttgatca tgttggtgac caccctgtgg gccagggcct tgtccggt
//
LOCUS       DAOQQN010000319         3830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_11543_length_3830_cov_5.720265, whole genome shotgun sequence.
ACCESSION   DAOQQN010000319 DAOQQN010000000
VERSION     DAOQQN010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11543_length_3830_cov_5.720265"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PLG24_10880"
     CDS             <1..505
                     /locus_tag="PLG24_10880"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMZ25440.1"
                     /translation="IEKFLTERISSDKGDDMDEDVPSLKSKAKDKDGKASYSTPECLL
                     PLMQFEKPVDGEVTGDFDAGKFHYGIDLAAPSDAEILAVEDGLVVINGYSREDGHVLM
                     ISHANGYISWYKHNSRNLVMPGREVIKGQPIAIIGNTGENSSGPHLHFELWQNDVPLN
                     PRSFLKY"
     gene            705..1967
                     /locus_tag="PLG24_10885"
     CDS             705..1967
                     /locus_tag="PLG24_10885"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMZ25441.1"
                     /translation="MIQFTRYVMDNGLTVLLHRDESTPLVVVNMLYKVGSKHESIRKT
                     GLAHLFEHLMFTGTEAVPDFDLPIQRAGGENNAFTSNDITNYYSWAPSDNLEALLFME
                     ADRMARLSLDNEEFEIQKNVVVEEFYETTIDEPYGDMWHLISGMAYHRHPYRWPVIGK
                     RPEDIRKVSHLDAAKFYRHHYYPQNAILVVSGNFKEERARKLIKKHFGTLINPDPHSD
                     SIPKEGVQRKQRRMTVHRKVLVQALYMAFHIPARYDKDFYACDILSDLLASGRSARLY
                     EILVRQKKLFTAIDAYTNGTSDEGLLIIDGRLSEGVTPEEGEAAVWEILEELVIKPVE
                     ERELQKVINKCITTLVFSEYNATNIAMNLAYFESIGNADLINSEFEIYQQLTVRDIRR
                     AAEKVFRKSNSSILYYLSANGHGANLVH"
     gene            1945..2355
                     /gene="ruvX"
                     /locus_tag="PLG24_10890"
     CDS             1945..2355
                     /gene="ruvX"
                     /locus_tag="PLG24_10890"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HMZ25442.1"
                     /translation="MGRILCIDYGTKRCGIAATDVLQIAVHAVGTVPSGELWLFLEEY
                     LSKEEVEKIVIGYPTHSDGSEFSFMHQIVGLERKIKKIFPQIELDRHDEAFSSARAKE
                     VIIHSGIRKEKRKDKGLVDKISAVLILQDYLKHI"
     gene            2380..2937
                     /gene="def"
                     /locus_tag="PLG24_10895"
     CDS             2380..2937
                     /gene="def"
                     /locus_tag="PLG24_10895"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HMZ25443.1"
                     /translation="MILPIYAYGQPVLKREGDEVSREYPELDKLIDDMFETMYNASGV
                     GLAAPQVGLSLKLFVVDTIQVEKEETRGKGIKKVFLNAELLEETGDEWAYEEGCLSIP
                     DVRGEVDRLPVIRLRYQDEKFEVHEETFEGINARVIQHEYDHTIGKLFIEKLGPVKKQ
                     LIKRKLENIKRGKVSADYRLKFAKF"
     gene            complement(3013..3648)
                     /locus_tag="PLG24_10900"
     CDS             complement(3013..3648)
                     /locus_tag="PLG24_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HMZ25444.1"
                     /translation="MAKKKPKFYVVWKGRNVGIFDTWDDCKAQVFGFEGAQYKSFDSR
                     EEALTAFQSGYHASIKSPKSIKTSTAKISRTAIVPSSISVDAACSGNPGMMEYRGVMT
                     DTGKEIFHLGPLRDGTNNIGEFLALVHVLALLKQKGREDIPIYSDSKIAIGWIKKGKA
                     NTKLEHTGHNEPIFQLIQRAERWLANNSFANPILKWETDSWGEIPADFGRK"
BASE COUNT         1133 a          727 c          902 g         1068 t
ORIGIN      
        1 gattgagaag tttctcacgg agcgtatctc atcggataaa ggagacgaca tggatgaaga
       61 tgtaccttct ttgaagagta aagcaaaaga taaagatgga aaagcgagct attcgactcc
      121 ggagtgcctc ttgccattga tgcagtttga aaagcctgta gacggagaag tgacgggtga
      181 ctttgatgca ggtaagtttc actatggtat agaccttgct gcaccctctg atgctgagat
      241 attggctgtt gaagatgggc tggtggtcat caatggatat tccagggagg atggccatgt
      301 tttgatgata agtcatgcca atggttatat cagctggtat aagcataatt cccggaatct
      361 tgtcatgccc ggaagagaag tgatcaaagg gcagcccata gccattatag gcaatacagg
      421 agaaaattct tcagggccgc atctccattt tgaattatgg cagaatgatg tcccattgaa
      481 ccccagatca tttttgaagt attgattgaa accagcatga aatgagccct tctcttttta
      541 tagagctgta aatgcttcat tgcatcgggt ctccaatttg ccagtaacga tttcctttca
      601 aaaaagcatc gaataccata aattatttga agcaaatcct ttgtatgcta atatgaggga
      661 attgaaattc tccattttag tcccacatcc ttatctttgc ccgcatgatt caatttacgc
      721 gctatgtaat ggacaatgga ctgacagtat tgctccatcg tgatgagtcc actccgctcg
      781 tcgtggtcaa tatgctttat aaggtcggtt ccaagcatga atcaatacga aaaaccggac
      841 tggctcacct ttttgaacac ctgatgttta ccggcactga ggctgtaccg gacttcgacc
      901 tgcctatcca gcgtgcaggt ggggaaaata acgcctttac ctccaatgac atcactaatt
      961 attatagctg ggcaccgtca gataatctgg aggcattact tttcatggaa gcggacagga
     1021 tggcacgact tagtctggac aatgaggagt ttgaaatcca aaagaatgtt gttgtcgagg
     1081 aattctatga gacaacgatt gatgaaccgt atggcgatat gtggcatctc atatcaggca
     1141 tggcttatca caggcatcct tatcgctggc ctgtcatagg gaagcggccc gaggatatac
     1201 gtaaggtaag ccacttggat gcagccaaat tttataggca tcattattat ccacaaaacg
     1261 ccattttggt ggtgtctgga aatttcaaag aggaaagggc caggaagctg attaagaaac
     1321 acttcggaac actgatcaac ccagatcccc attcagattc aattccgaaa gaaggagtgc
     1381 aacggaaaca acgccgaatg acggtgcata gaaaagtgct cgtacaggca ttgtatatgg
     1441 cttttcatat tcctgcgaga tatgacaagg atttttatgc ctgcgacatc ctttctgacc
     1501 tgctagctag tggtcgttct gcgagactgt atgagatttt ggtacggcaa aaaaaactgt
     1561 ttactgccat cgacgcttat accaatggca ccagtgacga aggcctgctc atcattgacg
     1621 gtagattgtc tgaaggtgtg actcctgaag agggcgaggc tgctgtatgg gaaatattgg
     1681 aggaactagt gataaaacct gttgaggaaa gggaattgca aaaggtaatt aacaaatgca
     1741 ttacgacgct tgttttttcg gaatacaatg ctaccaacat tgcaatgaac cttgcttatt
     1801 ttgagagtat aggtaatgca gacctgataa acagtgaatt tgaaatctac cagcagctaa
     1861 cggtccgcga tatacgcagg gctgcggaaa aagtattcag aaaatcgaac agcagtattt
     1921 tatattactt atcagcaaat ggacatgggg cgaatcttgt gcattgatta cggaaccaag
     1981 cgttgtggta tagctgctac cgatgttctg cagattgctg tacatgccgt tggcacggtt
     2041 ccgagtggag aattatggct atttctagaa gaatatcttt ctaaggagga agtcgaaaaa
     2101 attgtcatcg gctatccaac acatagtgat ggctcagaat tttcgtttat gcatcagatt
     2161 gtcggcttgg aacgtaaaat aaaaaaaata tttcctcaga ttgaattgga tcgacacgat
     2221 gaggcgtttt catcggccag agccaaagag gtcatcattc attcaggtat ccgaaaggag
     2281 aaaagaaagg ataaaggttt ggtggataaa atttctgctg tgttaatttt gcaggattat
     2341 ttaaaacata tttgacagga taataattat atatagacta tgattttacc aatatatgca
     2401 tacggtcaac cggtattgaa gagggaaggt gatgaagtat caagggaata cccggagctg
     2461 gacaaactga tcgacgatat gtttgagaca atgtacaatg caagtggtgt cggtttggct
     2521 gcgcctcagg taggcctgag ccttaagctt ttcgtggtgg acacaattca ggtggaaaag
     2581 gaagaaacca ggggcaaggg catcaaaaaa gtatttctca atgctgaatt gctggaggaa
     2641 acgggtgacg aatgggctta cgaagaaggt tgtctttcaa tccctgatgt acgtggagaa
     2701 gtggataggc ttcctgttat cagattaaga tatcaagacg agaagtttga ggtgcacgaa
     2761 gagacttttg aaggcatcaa tgcccgtgtg attcagcacg aatatgatca cactatcgga
     2821 aaattattta tcgaaaagtt aggtcctgtc aaaaagcaac tgatcaagcg aaagctggaa
     2881 aatatcaaac ggggtaaggt aagtgccgat tacagattga aatttgccaa gttctgaggt
     2941 tggcgggaac aatgtcgatg tgagattgaa ttgcaaacac tgagtaacca ttgaattgct
     3001 tactcactgc actcattttc tgccaaaatc agccggtatt tctccccaac tatcagtctc
     3061 ccatttcagg atcggattag caaaactgtt gttggcgagc catctttctg cccgctgtat
     3121 gagttgaaag atgggttcat tgtgcccggt gtgttccagt ttcgtattgg ctttaccttt
     3181 tttaatccaa ccgatagcga tcttactgtc ggaataaata gggatatctt ccctcccttt
     3241 ctgtttcaat agtgccagaa catgcaccag cgcaagaaat tcaccaatat tgttggtccc
     3301 atcacgcaat ggaccgaggt ggaatatttc tttgccggta tctgtcataa caccacgata
     3361 ttccatcatt ccgggattac cactacatgc ggcatcgaca ctaatggagg aaggaacgat
     3421 agctgttctg gaaattttcg cggtagaggt cttaatactc ttaggactct taatgcttgc
     3481 atgataacca ctctgaaagg ctgttaatgc ctcttccctt gagtcaaagg atttatattg
     3541 agcaccttcg aagccgaaca cctgagcctt acagtcgtcc caggtatcga aaatgcctac
     3601 attgcgacct ttccagacaa catagaattt tggttttttc ttggccaaat ttttgtgaat
     3661 tttacgacta aagataagga gaaacaagtt ttttgaatgg gaattgtttg gctgtgggtg
     3721 taaaatgttg tactctgcgt ccatgcagat gtaaagctgg aaatatatgc atattataaa
     3781 cccagattac gcaataggat ttttttgatt aaaaaaaaag gtgtaatttt
//
LOCUS       DAOQQN010000320         3799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_11699_length_3799_cov_4.557158, whole genome shotgun sequence.
ACCESSION   DAOQQN010000320 DAOQQN010000000
VERSION     DAOQQN010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11699_length_3799_cov_4.557158"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1979
                     /locus_tag="PLG24_10905"
     CDS             <1..1979
                     /locus_tag="PLG24_10905"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HMZ25445.1"
                     /translation="NTLYYTRSGNFIYQYDAKKNTTDSVDIVALLNPALKAAGYDELK
                     SAPRFKLGDMDEFEFSSGRDRLSFNMKDKKLTKVVTTPDDADMLDGTGNAFSFSRNQA
                     LYVQKPDGNVVMIAQGSDSILYGTSVHRDEFGISKGTFWSPKGNKLAFYRMDQSMVEK
                     YPIPDWSVTPAKVNDIYYPMAGRKSHHVTVGIFDLKTEKTIYLETGGDPEHYLTNICW
                     APDESEIYIAEINRDQNHTTFNAYNPDRGNKIRTLYEEQSLQYTEPLVPFTFVPGQND
                     LFIAQSKRDGWNSLYLYTHEGELVRKLTKGIEVTGLLGFDKKNKTAYFQGILPNSIDG
                     QYFSCDLATAKVTQHTNTPGQHTAIISFDGKYAFETNNALHSNISYTCRELGKNKVRT
                     LNTLAEPLDNYNLGNIKLDTLYSAGGDKLFTRTIFPYNFDPAKKYPVVIYVYGGPHAQ
                     MISNSRNAQAPLWMFTMANEGFIIFTLDNRGSSNRGMEFEKVIHRNLGNSEIQDQMVG
                     YEFIKNKPYVDAAHIGVHGWSFGGFMTISLMTRQPGKFYAAVAGGPVTDWNMYEVMYG
                     ERYMDTPQQNPEGYKQASTFTYIDQLQGPLLIIHGALDDVVVWQQSMKYIDTCIKKGK
                     QVDYFVYPGHKHNVLGKDRVHLIRKVIDYFEQHL"
     gene            1995..3776
                     /locus_tag="PLG24_10910"
     CDS             1995..3776
                     /locus_tag="PLG24_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ25446.1"
                     /translation="MTKETTLTGSGKYLGYFQSLVGSRIYLSLALSVVITLFDSVGIG
                     LLVAILQFMANSENGDSSLSLNAYYKQVFGQDATFLMLILIAIFIFLLKGLLTFTLYS
                     VQARMTTTIISKTRHNILDSIVNLNFASFTKTDLGTIQNISTSEVGRLNIALNSYLKT
                     GQYLIMSVVYLALAIVFDPVFSFLIIFTALAVLYLYNFIIKYFKKLSRQILSSRNQYS
                     SFLTQLLTNYKYLKTTDTIDKYSRKVKDQIDHTESLNYRFLKINAITDSAREPIILVM
                     ISLAIILLFHVKGGIGISMIFTMLLFYRTLNNVLLIQANWQGFHQNSGSVDRIISIQE
                     EFSKDMEAIGTDSAFHIAGRISLEKVDLNLSDKQILSDISLTLEKNSTTALVGPSGAG
                     KTTLASVITGLIQPNSGNISINNRLLESSDLKGYRRKIGFVTQDPVIFNDTLFNNITL
                     WEEKNSQSVKRFSEICRMTHLEDFVQAQLDKEDMQLGDFGITLSGGQKQRISIARELY
                     RDVELLVFDEATSSLDAITEQIIRENIQSIRGKVTIILIAHRLSTIRFADRIYFIEGG
                     RITASGTYDELMQTSPDFLKMVNLQSG"
BASE COUNT         1127 a          836 c          792 g         1044 t
ORIGIN      
        1 ccaacacact ctactatacc cgcagtggaa attttatata tcaatacgat gccaaaaaga
       61 atacgaccga ttctgtggat atcgtcgccc tcctcaatcc ggcacttaaa gcagccggat
      121 acgatgagct gaaatccgca ccaaggttta aactgggcga catggatgag tttgagttta
      181 gcagcggaag ggatcgactt tcctttaata tgaaggataa gaagctgact aaggtcgtta
      241 cgacaccgga tgatgcagat atgcttgatg gtaccggcaa tgctttttca ttttcacgta
      301 atcaggcgct ttatgtacag aagcccgacg gaaatgttgt tatgatagct cagggcagcg
      361 attccatcct ttatggcact tctgtccacc gcgacgaatt cggcatcagc aagggtactt
      421 tctggagccc caaaggcaat aaactggcct tctatcgcat ggatcagtcg atggttgaaa
      481 aatatcccat ccctgactgg tccgtcacac ccgctaaagt caatgacatc tactacccga
      541 tggcaggtcg caaaagtcat cacgttaccg tggggatttt tgacctgaag accgaaaaga
      601 ccatttatct ggagaccggc ggagatcctg aacactacct gaccaatatc tgctgggcac
      661 ccgacgagtc tgaaatctat atcgctgaga tcaaccgcga ccagaatcac accaccttca
      721 acgcctataa ccccgacaga ggcaataaaa tcaggacact ctacgaagaa caaagcctgc
      781 aatataccga acccttagtc ccctttacct ttgttccggg tcagaatgat ctatttatcg
      841 ctcagagtaa gcgtgacggc tggaattcac tctacctcta tacccatgaa ggtgagctgg
      901 tacgtaagct gaccaaagga attgaggtta caggtttact tggttttgac aagaaaaaca
      961 agacagctta ttttcagggc atcctgccca attctataga tgggcagtat ttttcctgcg
     1021 atttggcaac tgctaaggta acgcaacata ccaatactcc cggtcagcac actgccatta
     1081 tatcctttga cggaaaatat gccttcgaaa ccaacaacgc cctccacagt aatatttcct
     1141 atacctgccg agaactgggt aagaacaagg tgcgcaccct caatactctt gcagaaccat
     1201 tggacaacta taacctggga aatatcaaac ttgacacact ctattctgcc ggaggtgaca
     1261 agttgtttac aaggacgatc ttcccctaca attttgatcc ggccaaaaag tatccggtcg
     1321 tgatttatgt ctatggtggc cctcacgcac agatgatcag caatagccgt aatgcgcagg
     1381 cacccttgtg gatgtttacc atggccaatg aaggctttat tattttcaca ctggacaaca
     1441 gaggttcatc caatcgcggc atggaatttg aaaaagttat ccacaggaac ctgggaaact
     1501 ctgagattca ggatcagatg gtgggttacg aatttatcaa aaacaagccc tatgtcgatg
     1561 ccgcccatat aggtgttcac ggatggagct ttggcggatt tatgaccatc agtctgatga
     1621 cccgccagcc gggtaaattt tatgctgccg tcgccggcgg acctgttacc gactggaata
     1681 tgtatgaagt tatgtacggc gaaagataca tggatacacc tcagcaaaac cccgaaggat
     1741 ataagcaagc aagtactttt acatacatag atcagttgca ggggccgctg cttattattc
     1801 acggtgcctt ggatgatgtt gtggtatggc agcagagtat gaagtatatc gatacttgta
     1861 tcaaaaaggg caaacaggtt gattattttg tctatccggg acacaagcac aatgtgctgg
     1921 gcaaagacag agtacacctg atcaggaaag ttattgatta ttttgagcaa catttataat
     1981 ttcaagtata ctgattgacg aaggaaacca cactgaccgg ctccggaaaa tatctcggtt
     2041 attttcagtc acttgtaggt agccgtattt atttatcact ggctttatct gttgtcatta
     2101 cactctttga cagtgtaggg attggattgt tggtcgctat actccaattt atggcaaata
     2161 gtgagaatgg agattcttct ctcagcttaa atgcttatta caagcaggtt ttcgggcaag
     2221 atgctacatt tttgatgtta attttgattg ctatttttat ttttttattg aaaggtttac
     2281 tcactttcac cctatattcg gttcaggcga ggatgactac caccatcatc agcaaaacca
     2341 gacataatat actggatagt attgtcaatc tcaattttgc atcatttaca aaaaccgact
     2401 tagggacgat acaaaatatc tcaacctccg aagtaggaag gctcaacatc gctttaaaca
     2461 gctatttgaa gaccggacaa tatttgataa tgtccgtagt atatctggca cttgcgatag
     2521 tttttgaccc tgttttttct tttttgatta ttttcacagc tttggcagta ttgtatctgt
     2581 ataattttat aataaaatat ttcaaaaagc tatccagaca gattctgagc tccagaaatc
     2641 aatatagtag ttttctaact cagcttctga cgaattataa atatctgaaa actacagaca
     2701 ctattgataa atacagtagg aaggtcaaag atcagataga ccatacagaa tcgctaaatt
     2761 acagattcct taagatcaat gccatcactg atagtgccag agaacctatt attctggtca
     2821 tgataagcct ggccattata ttattgttcc acgtaaaagg aggcatcggt attagcatga
     2881 tcttcaccat gctcttattt taccgtacgc ttaataatgt cctattgatt caggccaatt
     2941 ggcagggatt tcatcagaat tcaggaagcg ttgacagaat tatttctatt caggaggaat
     3001 tttcgaaaga tatggaagca attggaacag acagtgcctt tcatatagcc ggtcggattt
     3061 cattggaaaa ggtagatctt aatctttcag ataaacaaat tcttagcgac atcagcctga
     3121 cgcttgagaa aaattctaca actgcattgg tagggccaag tggcgcagga aagacaactt
     3181 tggcgtctgt cataaccgga ttaattcaac ccaattccgg aaacatatcc ataaataatc
     3241 gacttctgga atcatctgac ctgaaaggat atcgccggaa aattgggttt gtaactcagg
     3301 atcccgttat attcaatgat actttgttta acaatatcac tctttgggaa gaaaaaaaca
     3361 gtcaatctgt caaaagattt agtgaaatat gcaggatgac gcatctggag gactttgttc
     3421 aggctcaact tgataaagaa gatatgcagc tgggcgactt cggcatcacc ctatccggtg
     3481 gccagaaaca acgaatcagt attgcaaggg aattgtaccg tgacgtggaa ttgcttgtct
     3541 ttgatgaagc tacctcatct ttggacgcca tcaccgagca gattatccgg gaaaatatcc
     3601 agtccatccg cggcaaggta accatcatcc tcatcgccca ccggctgtct accatccgat
     3661 ttgccgatcg gatttatttc atcgaaggag gtcgtatcac tgcttccggc acctacgacg
     3721 aactgatgca aacatcaccc gacttcctta aaatggtcaa cctgcaatcc ggctaatatg
     3781 cagcaagtta ccatcagtg
//
LOCUS       DAOQQN010000321         3785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_11772_length_3785_cov_5.548794, whole genome shotgun sequence.
ACCESSION   DAOQQN010000321 DAOQQN010000000
VERSION     DAOQQN010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11772_length_3785_cov_5.548794"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PLG24_10915"
     CDS             complement(<1..285)
                     /locus_tag="PLG24_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25447.1"
                     /translation="MKYFASLLGILFLFLFSTTGFSQDDLPRIELVKVFSGLSKPVSL
                     QSCNDNRIFIVEKGGKIKIGFPDGSVLDSNFLNISDKVLSGGERGLLGLAF"
     gene            791..1897
                     /locus_tag="PLG24_10920"
     CDS             791..1897
                     /locus_tag="PLG24_10920"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HMZ25448.1"
                     /translation="MNINQPEKMTLLEKIKVKRPFIIAGPCSAETEQQVIDTAVNLSR
                     DGNVDVLRAGIWKPRTRPGSFEGVGTVGLPWLQKAKEITGLPVAIEVANAKQAEEALD
                     HEIDIVWIGARSTTNPFSVQEIANVLKGVDIPVWIKNPINPDLELWTGAVERIQQSGI
                     RTIGLIHRGFHSYGDNEYRNTPMWNLAIEMRRRYPELLFINDPSHICGNRHMLAEVAQ
                     TAVDLAYDGLMIECHIDPDHAWSDSAQQITPDGLSKLLKKIHWVQFIENGETDEQLIR
                     LRHQIDHMDDQIIELLSQRMVISDAIGAYKKEHNLSILQMKRWNEIMDRISRKGKDLM
                     LSNEFIHQYFNAIHMESIRRQNKVINKKIDHLNN"
     gene            2068..>3785
                     /locus_tag="PLG24_10925"
     CDS             2068..>3785
                     /locus_tag="PLG24_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HMZ25449.1"
                     /translation="MHRIFNTIIWITFTLASYVEVQAQTMDSLYHAVEVDLSTTSIQE
                     IARLGIEADHGIYAPGRFWINYLSDYEIGLLQSNKISFRRIDRSGMKVFDRSGDNCFN
                     TNPNKSDPRNFRLGKMAGYYTLEEMNAILDSMHLLFPQLISTRKDISTFRTFEDRPIQ
                     WLRISNHPEKDQEKPRVLYTALHHAREPMSLTQLIYFMWYLLEEYANNEEIRELIDNT
                     ELYFIPCVNPDGYAFNASNKPEGGGMWRKNRWLHPSGFIGVDLNRNYGLAWGIDDVGS
                     SPDPFSSVYRGPGPFSEPETQAVAYLTDQYKFDLVLNSHTYGNYIIYPWNYTADPCPD
                     DEGFKAIARVYAGRNSFAIGNSKETVGYLSNGGSDDWIYAHDPEHKIYSFTPEVGFNS
                     EGFWPDASRIRQISGNSLEGNIVFAEMAHRYFDIAFLDPLFLIRNKDNEINFSISRAG
                     SGQGPITLKFRILDNSAILDKNRLDYDMPVPGLRVDRIVITPGTTAMNGDPVKLEITR
                     EMGLLTVKDTVEFTVKSGISTIINKCDDISTIIPDGVDWGEDYKEFYSPPSSFADSPD
                     GFYREN"
BASE COUNT         1215 a          779 c          780 g         1011 t
ORIGIN      
        1 aaaggcaaga ccaagcagcc cacgctcacc acctgacaac accttatctg aaatgtttag
       61 aaagttagaa tccagcactg aaccatcagg aaagccaatc tttattttgc cgcccttctc
      121 tacaataaaa atcctgttat cattgcagct ctggagggat acaggttttg aaagccctga
      181 aaatacttta accaattcaa tacgtggcaa atcatcctga gaaaatccgg tcgtactgaa
      241 taagaacaaa aaaagaattc ctaatagact agcaaaatat ttcatatttt tctgagttta
      301 aagctttaaa aatatttcat tttttcaaat tttcacaatt tattactcat tttgagtgca
      361 aaatttaggt aacatatatt tactcatttt atataaaact ttaaatcaaa cgtttaaata
      421 tcacatattt taaccaaccg ttgattaaca ccctgcaccc aataccattg tcatgattta
      481 caatgaatta gtgtagttat tattgaactt taggtgatga ttcatcctgt taactctctt
      541 ttgtcaaccg gctttttctg ccaaaaatcc caatccgcct cgcttgatgt ccaaaatgaa
      601 tatcatccct attaaaccct ccatcattca tcctttatta aaaattatag cagaatattt
      661 cagcggatga taacttgact ttggagagta tccttgacaa ctccactcaa actaaaaaat
      721 tcatgcgaat cctttaacct taagtacctt tgttccattt aaatattaac tccaccgcta
      781 aggttaagta atgaatataa accagcctga gaagatgacg ctcctcgaaa aaatcaaggt
      841 gaaaagacct tttattatag caggcccttg tagtgccgaa actgaacaac aggtcattga
      901 cactgcggta aatctaagcc gggatggcaa cgtcgacgta ttaagggcag gaatatggaa
      961 gcccaggaca aggcccggca gctttgaagg agttggaact gttggtttac catggttaca
     1021 gaaggcaaaa gaaattacag gtttgccggt ggcaatagag gtggcaaatg caaagcaggc
     1081 agaagaggca cttgaccatg aaatagatat tgtgtggatc ggagccagaa gtacgaccaa
     1141 tcctttcagt gtccaggaaa ttgccaatgt cctgaaagga gtcgacattc ctgtctggat
     1201 caagaatcct atcaatccgg acctggaact ctggaccggc gccgtagaac ggatacaaca
     1261 atcgggtatt cggacaatag ggcttataca ccgaggtttc cattcttatg gagacaatga
     1321 ataccggaat acacctatgt ggaatctggc aattgaaatg agaaggcgat accctgaatt
     1381 gcttttcatc aatgatccgt cacacatctg cggcaacaga catatgctgg ccgaagtggc
     1441 ccagactgcc gttgatctgg cttatgatgg cttgatgata gaatgtcata tagaccccga
     1501 tcatgcatgg agtgattcag ctcaacaaat cacgcctgat ggcctgagca aattattgaa
     1561 aaaaatccac tgggtacagt ttattgaaaa tggagagaca gacgagcaat tgattagact
     1621 tcgtcaccag atcgaccata tggatgatca aatcatagag ttgcttagcc aaagaatggt
     1681 gatctcggat gcaataggcg cgtacaagaa agaacataac ttatccattc tccaaatgaa
     1741 aagatggaat gaaatcatgg accgaatcag caggaaagga aaagatctca tgctgagcaa
     1801 tgaattcatc catcaatatt tcaacgccat acatatggaa agcatccggc gtcaaaacaa
     1861 ggtgattaat aaaaaaattg accatcttaa caattaaaag gaaactaatt ccggctctct
     1921 gcacattgac taaataatca ggggcgtaaa accaaacccg aaaatttcag aatgaattga
     1981 ccaatcaatt atttttaagg caaatttttg aagtaaatat tacatattat atacaattat
     2041 ttatctttga ttattaatat tatataaatg catagaatat tcaatacaat aatctggata
     2101 acattcacat tagcttccta tgtagaagta caggcccaga caatggattc gctttatcat
     2161 gccgtggagg tggatttaag tacgacatct attcaggaaa ttgcccgttt ggggatagaa
     2221 gctgatcatg gtatttatgc tccgggccga ttttggatca attatttgtc agactacgaa
     2281 atcgggctgt tacaaagcaa taaaatatcg ttccgaagaa tagaccgttc cggaatgaag
     2341 gtttttgaca gaagtggaga taattgcttt aataccaatc ccaacaaatc cgatcccagg
     2401 aatttccgat tgggtaagat ggccggatat tatacactcg aggaaatgaa tgccatcctg
     2461 gacagtatgc atcttttatt cccacaactt atttcgacca gaaaagacat atcaacattc
     2521 agaacctttg aagacaggcc aatacaatgg ctccgcatat ctaatcatcc cgaaaaagac
     2581 caggaaaagc cccgcgtcct atacacggca ctccatcatg ctagggagcc gatgagcctg
     2641 acccagctta tttattttat gtggtacctt ttggaggaat atgccaacaa tgaagaaata
     2701 agggaattaa tagacaatac ggaattatac ttcattccct gtgtcaatcc ggacgggtat
     2761 gccttcaatg caagtaacaa accggaaggg ggaggtatgt ggcgtaaaaa cagatggttg
     2821 catccttcag gattcattgg tgtagatctc aaccgcaatt atgggcttgc ctggggcatt
     2881 gatgatgtcg gctcaagtcc ggacccattc agtagtgtgt accggggacc gggtccattt
     2941 tctgagccgg agacacaggc agtagcctat ctgactgatc aatacaagtt tgaccttgtg
     3001 ctcaatagcc acacttatgg caattacatc atctatccgt ggaactatac tgccgatcca
     3061 tgtcccgacg atgaggggtt caaagctatt gcaagggtat atgccggaag aaacagtttt
     3121 gccataggta acagcaagga aaccgtcgga tatctttcaa acggaggatc tgatgactgg
     3181 atatatgcgc atgatcccga acacaagata tattcattta ctccggaagt aggattcaat
     3241 agtgaaggat tctggccgga tgcctcgcga atcaggcaga tttccggaaa cagtcttgaa
     3301 gggaatattg tttttgctga gatggcgcat agatatttcg acattgcttt tcttgacccg
     3361 ctctttctga ttcgtaacaa ggataatgaa atcaattttt cgataagccg tgccggttcc
     3421 ggacagggac cgattactct aaaatttcgg atactggaca attcggcaat tctggacaaa
     3481 aaccggttgg actacgatat gcctgtcccg ggcctgcggg tggacagaat agtcatcact
     3541 cccggcacaa cagcaatgaa tggcgatccg gttaagttgg agatcaccag agaaatgggt
     3601 ctgctcaccg taaaggatac tgtagaattt accgtcaagt cagggatttc gactatcatc
     3661 aataaatgcg atgacatatc aaccatcatt ccggatggag tggattgggg agaggattac
     3721 aaggaatttt actcaccccc gtcttcattt gcagattctc cggatggatt ctaccgtgaa
     3781 aatca
//
LOCUS       DAOQQN010000322         3779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_11800_length_3779_cov_4.572234, whole genome shotgun sequence.
ACCESSION   DAOQQN010000322 DAOQQN010000000
VERSION     DAOQQN010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11800_length_3779_cov_4.572234"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2032
                     /locus_tag="PLG24_10930"
     CDS             <1..2032
                     /locus_tag="PLG24_10930"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HMZ25450.1"
                     /translation="ENDNFNIFHVTSSNNTWNAKGAVSAHYNGGIAYEYFKNTFGRNS
                     INGSGGTIISVINVSENDGSGMDNAFWNGKAMFYGNGAQSFKPLAGGLDVAGHEMSHG
                     VIQATANLTYQGESGALNESFADIFGVLIDREDYKIGEDVVKSGVFPGGALRDMSNPH
                     NGGSKLGDRGWQPNHMNEKYTGSQDNGGVHINSGIVNFAFYKIASKIGKDEAEKIYYA
                     ALTKYLTKSSQFIDCRNSVIQAAKDIYGTGSQNISIIENAFAEVGIGSGSGTPEQPDY
                     GTNPGQDYLLFASSDLNQIKLVNFSSGSATTLNHDKGLASKISVSDDGTLAVFVGKDN
                     ALYYIQFNWSAGNYNIGILDNQNQWRNAAISKDGLRLAALLDIEEPVIYVFNLESDTY
                     KAFELYNPTTSSGSIFTNDVKYADALEFDHSGEYLMYDALSSLGFFGTEYWDIGFLNV
                     WNNSTSNFAEGYIEKLFSSLPDGVSVGNAVFSKNSPSIIAFDYFEDGSSTVNELLGAN
                     IETGELNVLFENSVLGFPCFSRDDKNIIFNANDNNGKSVIAIIPLEDDKISPALGASI
                     LVNNSTLGTWFANGKRVINSTQQYNALEDLVTIFPNPATNIISVRWNSNHGISGTINV
                     YDTQSKKLFSKNVSLQSGDNLYTIPVDNLIPGAYYIELIKGNQSKSISIIKK"
     gene            2142..3095
                     /locus_tag="PLG24_10935"
     CDS             2142..3095
                     /locus_tag="PLG24_10935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HMZ25451.1"
                     /translation="MNPAIKVLIVRFSSIGDIVLTTPVIRCIKQQLGAEVHFLTKPGF
                     AGLLTDNPYIDQLWTLEEDYIGLIRKIKEERFDYLIDLHKNLRTWWLAFQLGVKRLTY
                     HKATIEKLLMVKFKINVLPKSHVSERYLDAVKSLGIKYDGQGLDYFISEDTVNKLDGV
                     DSLYIAAVIGATHFTKRLPEENWITYINSTDRLIVLIGGKAEAEAAEHIVARSGSNVI
                     NMVGKTNLQESALIIQNAERVITNDTGMMHIAAALGKPIVSIWGGTLWEYGFWPFYSD
                     GKNLNKSLEVKGLSCRPCSKFGRNDCPRKHFKCMKDLKIEL"
     gene            3113..>3779
                     /locus_tag="PLG24_10940"
     CDS             3113..>3779
                     /locus_tag="PLG24_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ25452.1"
                     /translation="MPNSKSIWTTSKNESQSVIDSAVEGDRVYVTTDKNNNFIDATSI
                     YGTSILGYGNKKIAHAIFDQLMKLEEVDTAWFNHNIIGTLATDLLSKGGMPNGKVNFF
                     QDRFLALEAIICMAIRYQYTSGKERSKLMVWSNSTFTNKLKSSILMASALPKYIPENQ
                     LPEIVTIPLPSSPDDMEAFNEIEILLQKEEIAAFIYEPLIQCDRYMTVYSKEVLSKTL
                     ELTK"
BASE COUNT         1235 a          713 c          763 g         1068 t
ORIGIN      
        1 tgaaaatgac aattttaaca tatttcatgt aacatcatcc aataatacgt ggaatgctaa
       61 aggcgcagtc tccgcccatt acaatggtgg aatagcgtac gaatatttta aaaatacctt
      121 tggaaggaat tcaatcaatg gttcgggagg cacaataatc tcggttatca atgtatctga
      181 gaatgatggc agtggcatgg acaacgcatt ctggaatggc aaagccatgt tttatggcaa
      241 tggagctcaa agttttaaac ctctagccgg gggtctggat gttgcgggac atgaaatgtc
      301 acatggcgtc atacaggcta ctgcaaacct gacttatcaa ggtgaatcgg gagcactcaa
      361 cgaatctttt gcggatatat tcggagtgtt gattgatcgt gaggattata agatcggtga
      421 ggatgtagta aagtcgggtg tttttcctgg tggtgctctc agagatatgt ccaatcctca
      481 taatggagga tcaaaattgg gtgaccgggg atggcagcct aaccatatga atgaaaaata
      541 tacaggctct caagacaatg gcggagtgca tattaacagt ggtattgtca actttgcctt
      601 ctacaaaatc gcttccaaaa tcggtaaaga tgaagcagaa aaaatctatt atgctgcact
      661 caccaaatat ctgactaaat cctctcaatt tatcgattgc cgtaattccg tcatccaagc
      721 cgcaaaagac atatatggca caggttcaca gaacattagt atcattgaaa acgcttttgc
      781 cgaagtcggt ataggttcag gaagtggcac acctgaacaa ccagactatg gaacaaaccc
      841 cggtcaggat tatttgttgt ttgcaagttc ggatctaaac caaatcaagc ttgttaattt
      901 ttcttcaggg agtgctacga ccttgaatca cgacaaagga cttgccagca agatatccgt
      961 atctgatgat ggtacattgg ctgtgtttgt cggaaaagac aatgcacttt attatattca
     1021 attcaactgg tcggcaggga attataatat aggcattctt gacaaccaaa accagtggcg
     1081 aaacgctgca atttcaaagg acggcctgcg tctggcagca ctcctggata ttgaagagcc
     1141 tgtcatttat gttttcaatc ttgaatcaga cacctataag gcctttgaat tatataaccc
     1201 aacgacaagc tcaggcagca tttttaccaa cgatgtaaag tatgcagatg cattggaatt
     1261 tgaccatagc ggagaatatt taatgtatga cgctctttca tcccttggct ttttcggcac
     1321 tgaatactgg gacataggtt ttttgaacgt atggaacaat tctacaagta attttgcgga
     1381 aggctacatt gaaaagttgt tttcttcact ccccgacgga gtaagtgtcg gcaatgccgt
     1441 tttttcaaaa aacagcccgt caataatagc atttgattac ttcgaagatg gaagctcaac
     1501 agtaaatgaa ctattgggtg caaatattga aacaggagaa ttgaatgtgc tctttgaaaa
     1561 ttcagttctt ggctttcctt gtttttccag ggatgataaa aacattattt tcaatgccaa
     1621 tgataataat ggaaaaagtg tcattgctat aataccgttg gaagatgaca aaatatcacc
     1681 tgccctcggg gcatcgattt tagtgaataa cagtacactg ggcacttggt ttgccaatgg
     1741 aaaacgtgtg atcaatagta ctcaacaata caatgcattg gaggatttag taactatatt
     1801 ccccaaccct gctaccaata taatttcagt cagatggaat tccaatcatg gcatctcagg
     1861 caccattaat gtttacgata cccaaagtaa aaaacttttt tcaaaaaatg tcagtttaca
     1921 atcaggagat aatttatata ctataccggt tgacaattta attcccggtg catattatat
     1981 tgagttaatt aagggcaatc aatcaaaatc aataagtatc attaaaaaat aatttattga
     2041 ttctgaatac aaaagtcctt ctttacctcg tgtagagagg gactttttat tttaccctaa
     2101 gcaactagat atagagtatt tcccaaataa attctataaa aatgaatcct gctataaaag
     2161 tacttatcgt cagattcagt tcaattggcg acattgtatt gaccacaccg gtcatccgat
     2221 gtattaagca gcaactgggt gcagaagtcc atttcctgac aaaaccaggt tttgcaggcc
     2281 tgctgactga caacccatac atcgatcaac tgtggacact tgaggaggat tatatcggtc
     2341 tcatccggaa aataaaggaa gaaaggtttg attacctgat cgatctacat aaaaacttaa
     2401 gaacatggtg gcttgcattt caactgggtg tcaaaaggtt aacctatcat aaagcgacca
     2461 tagagaaact cctgatggtc aaatttaaga tcaatgtatt accaaagtcc catgtttcag
     2521 aaaggtattt ggacgcagtc aaaagtttgg ggataaaata tgacggacag ggcctggact
     2581 atttcatttc cgaagataca gtcaataagc tggatggcgt ggatagttta tatattgcag
     2641 cggtcattgg cgccacacat tttaccaaac gactacctga agagaactgg ataacctata
     2701 ttaattccac agaccgtctt attgttttaa tcggtggaaa agctgaggca gaagcagcag
     2761 aacatattgt tgcacgaagt ggttcaaatg tcattaatat ggtagggaaa acaaatcttc
     2821 aggaatctgc tctaatcatt cagaatgcgg aacgggtcat taccaatgac accggcatga
     2881 tgcacattgc tgccgccctt ggaaaaccta tagtttccat ctggggtggc accttatggg
     2941 aatatggctt ctggccattc tattctgacg gtaaaaatct aaacaagtca cttgaagtta
     3001 aaggtctctc ctgcaggccc tgttccaaat ttggtcgaaa tgactgtccc aggaaacatt
     3061 tcaaatgcat gaaagactta aaaattgaac tctaaaatga taacattata tcatgcctaa
     3121 ttccaaatct atatggacaa caagcaagaa tgaaagccaa tccgttatag attctgcagt
     3181 tgaaggagac agggtgtatg tcaccacaga caaaaacaat aatttcattg acgccacctc
     3241 catttatggc acaagtattt tggggtatgg caataaaaaa attgctcatg caatattcga
     3301 ccaattgatg aaattggaag aggtcgatac agcatggttc aatcataata tcattggaac
     3361 gctggcaaca gatttgttgt caaaaggggg aatgccaaac gggaaagtta atttctttca
     3421 ggatcggttt ctggcgcttg aagcaattat ttgcatggcc atccggtatc aatatacttc
     3481 aggaaaagaa agaagtaaac taatggtatg gagcaattcc acctttacca ataagctgaa
     3541 atcatccatt ttaatggctt ccgcattgcc aaaatatatc cctgaaaatc aactgcctga
     3601 aattgtaacc attcctctac cctcttcacc tgatgatatg gaggctttca atgaaattga
     3661 aattttactt caaaaggaag aaattgcagc ttttatatac gaaccattga tccaatgcga
     3721 cagatatatg acagtttatt caaaagaagt tctatcaaaa acattggaat taactaaaa
//
LOCUS       DAOQQN010000323         3770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_11844_length_3770_cov_5.342665, whole genome shotgun sequence.
ACCESSION   DAOQQN010000323 DAOQQN010000000
VERSION     DAOQQN010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11844_length_3770_cov_5.342665"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="PLG24_10945"
     CDS             complement(<1..1180)
                     /locus_tag="PLG24_10945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ25453.1"
                     /translation="MNIRIYGVLSVFTLISFFSCKSDQKQMVMPPPTIPTVEVVNKDV
                     VSYRTFPVSIEGKVNNAVRAKISGYIAQVFVDEGQMVTKGQPLFRLETNVQSQDANAA
                     RSGVSAAQANIEAAKASVSAAQVEVDKLTPLVERNIVSNVQLETAKANLMRAQGQLEQ
                     AKAARKQASSSYSAVAANVNYGIVRSPITGVVGAINFREGSLVGPGDQMPITTVSETS
                     EVYAYFSMNESQYFDFLEQTPGKDVSEKLKNMPEVDLFLPNGRQYAAKGKIKTVTGQI
                     NPQTGTIQFRATFPNKEQILSNGNSGKVQIPVMHKGVLVVPESAAFEQQGATYVYRVG
                     KDTVTNIPIKVIARVHNMVLVESGLKTGDIVVAQGTGKLKPGAAIKPVPTSMDSIVNA
                     I"
     gene            complement(1177..1671)
                     /locus_tag="PLG24_10950"
     CDS             complement(1177..1671)
                     /locus_tag="PLG24_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25454.1"
                     /translation="MVREKVKTELIEELAAHHMETSNLPPLASKIFAFLLVNYCRDGY
                     NFDELVDVFKVSKSSVSNSLNLLIQYNFIIQTNRIDERKRRYKITPDYLSIRLNKIIK
                     DLIRERQLSVKLLEFHQTEVNPPGPGEISKAEVYLNHLDTAIRNLSCTLERIDNITNA
                     NQLV"
     gene            2352..>3770
                     /locus_tag="PLG24_10955"
     CDS             2352..>3770
                     /locus_tag="PLG24_10955"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMZ25455.1"
                     /translation="MSKALSVLIALALILQGCTRDQSDFIPTTPIPEYTNDILGVVMG
                     DDDAPIVGAQVRFNGKITLTDIYGIYQFKHVEVSSDHNLLTIEQPGYFSTVRVFGSSG
                     NGRIALKNNLLPKKFEYFFDATNGGSVSEANITFQFPANAFVDKISNQPYEGQVKVAI
                     GYIDPLDPKALDQLPGSVGGLTESESEACLSNYGIIVIEAQGIIGVPLSLATGKTIRA
                     SLRIPGEKLGQASNSTKIWGFDNNLGYWRESGQAILNANNQSYEAQLNDLAFYAFAEH
                     RPAKKLTGRVIDEAGRPLAFASVRINDGQKGFENRAFTDDNGRYNVTVPANTPMIINI
                     NKSKGCTVSLTNDSKINAINENTTLPDLRVKLDGGSYTKISGKLSSCNGNPVHTGYAY
                     AVTEKGSWALLPVANGVFEGYLLNCNDNSNVTIIGVNSESNEYSNEYSFSSGAEINTG
                     VISACTQESDFVALSIPELNFDT"
BASE COUNT         1062 a          822 c          789 g         1097 t
ORIGIN      
        1 taatggcatt aacaatgctg tccatggatg ttggtaccgg tttgatggcc gcaccaggct
       61 tgagttttcc tgtaccctga gctacaacaa tgtctccagt cttgagtcct gattccacca
      121 acaccatatt atgaactctg gcaatgacct tgatcggtat atttgttact gtatccttgc
      181 ctacccgata tacgtaggta gcgccctgtt gttcgaaagc tgcggattcc ggcacgacca
      241 acacaccttt atgcatcacc ggaatctgaa ctttaccgct attgccatta ctcaatattt
      301 gttctttatt aggaaaagtc gccctgaact ggatagtacc tgtctgtgga ttgatctggc
      361 cggtgacggt ctttatcttc ccttttgcgg cgtattgccg gccattgggg aggaagagat
      421 ccacttctgg catgtttttc aatttctctg agacatcctt ccccggcgtc tgctccagaa
      481 aatcaaaata ctggctttca ttcatggaaa aataggcata cacttcgctc gtctctgaca
      541 ctgtggtgat cggcatctga tcgcccggtc caaccagact gccttctcta aaattgatgg
      601 cgccgacaac accggtaatc gggctcctta caatgccata attaacattg gctgcaacgg
      661 cgctatagga agacgaggct tgcttccgtg cagcttttgc ctgttccagt tggccctgag
      721 cacgcataag atttgctttt gcggtttcca actgtacatt actgacaata tttctttcca
      781 caagaggtgt cagtttgtcc acttccacct gggcagccga aactgaggcc ttggcagctt
      841 cgatatttgc ctgtgcagca cttacaccgg aacgtgcagc gttggcatcc tgcgattgta
      901 catttgtctc cagcctgaat aacggctgac ctttggttac catctgccct tcatcgacaa
      961 agacctgggc gatataaccc gagatctttg ctctgacagc attgttgacc ttgccctcta
     1021 tacttaccgg aaatgtcctg tatgagacaa catccttgtt gacaacctcg acggtgggaa
     1081 tcgtcggagg aggcatcacc atctgttttt gatcgctttt gcaggagaaa aaggagataa
     1141 gggtaaaaac cgacaaaacg ccgtatattc ttatattcat actaattgat ttgcatttgt
     1201 tatattatct attctttcaa gggtacaact cagattgcgg atagctgtgt cgagatgatt
     1261 gagatagact tctgctttgg agatttcgcc aggaccgggt ggattaacct ctgtttgatg
     1321 aaattccaac aatttgaccg acaactgtct ttcgcgtatc aagtctttta tgattttatt
     1381 caatcggatg gaaagataat ccggagtgat tttataccgc cgcttacgct catcgattct
     1441 attggtttga atgatgaagt tatattgaat cagaagattg agactattgg aaacagaact
     1501 cttgcttacc ttgaacacat caaccaactc atcaaaatta taaccatccc tgcagtaatt
     1561 aaccaacagg aatgcaaata tcttcgatgc caacggtggc agattagagg tctccatgtg
     1621 atgagcagcc aattcttcaa tcaattcggt tttcactttt tctctcacca tagatgatat
     1681 ttatttctta acttttgttt gttcggtaac aacggaacga aacgaatcaa agtgataaaa
     1741 tgtttgcaaa aagtcaaagt ttttaagaaa aacaggtcat ttattttcag ccttctgccc
     1801 ttaagggctg ttgatgagcc aagtttattg cgattgatag atatcctgtg ttgtccattt
     1861 caaaaacgct ttagagataa catacctgac atacctcaac atttaatacc aattggacga
     1921 acaattccaa taaacagacc gggctattct tgtgcctgcc ttcctatcaa attttgttac
     1981 atgaaaccgg ggggaaagtc atcattctac ccttgtccac ggattgtatg aacagaaaac
     2041 ttagcagcaa aacaccgctt tatgttctat ggtatttatt ttatgttatt tatagccact
     2101 tttatgttat tttataattt tatcaaaaaa gcacagacgc gaatcatcta acatctgaaa
     2161 acacatcagg tattggaaga acacaccgat agaacattga acaaatccat cctccacacc
     2221 tgccttgcag gacttataaa tgccttgcaa ctcagacttt tatagtatct ttataaaaaa
     2281 atctaacaaa aatttatttt cggacaacga aaggggttga ttaaaggtaa ttaggatatt
     2341 aaactgaaat gatgagtaag gcattatctg ttctcattgc ccttgcattg attttgcaag
     2401 gatgcacccg ggatcaaagt gattttatcc ccactacacc aattccggaa tataccaatg
     2461 atattctggg tgttgtgatg ggagatgacg acgccccaat cgtgggtgct caggtacggt
     2521 tcaatggtaa aattacattg acggatattt acgggatcta tcagtttaag catgttgagg
     2581 tgagttctga ccataatctt ctgaccatcg aacagcccgg gtatttcagt accgttaggg
     2641 tattcggttc gtcaggcaat ggtaggattg cgctgaaaaa caatttgttg ccgaagaaat
     2701 ttgaatattt ctttgacgca actaacgggg gctcagtatc ggaggctaac attaccttcc
     2761 aatttcctgc gaatgcattc gtggacaaaa tttccaatca accttacgaa ggccaggtca
     2821 aagtggcaat aggctacatc gatccccttg acccaaaggc cttggaccaa ttgccgggtt
     2881 ctgtcggtgg cttaaccgaa tcagaatcgg aggcttgtct tagcaattat ggtattatag
     2941 tgattgaagc gcaaggaatc attggtgttc cgcttagtct ggctaccgga aaaactataa
     3001 gggcaagcct tcgaatacct ggagaaaaac ttggtcaagc ttccaactcc actaagattt
     3061 ggggcttcga taacaatctc ggttattggc gcgaaagtgg acaagctatt ctcaatgcca
     3121 acaaccaaag ttatgaagct caattgaatg atttggcctt ttatgcattt gctgaacatc
     3181 ggccggccaa aaaactgacc ggaagggtga tcgacgaagc cggaaggcct cttgcctttg
     3241 cttcagttcg catcaatgat gggcagaaag gattcgaaaa ccgggcattt accgatgata
     3301 atgggcgata taatgtcacc gttcccgcca atacacccat gattatcaac atcaataaaa
     3361 gtaaaggctg cacagtaagc ctgaccaatg attccaagat taatgccatt aatgaaaaca
     3421 ccaccttacc tgatctaagg gttaagcttg atgggggtag ctatacgaag ataagcggta
     3481 aattatcatc gtgcaacggc aacccggtcc atacaggcta tgcctatgca gtgactgaaa
     3541 aaggttcatg ggctttactg cctgtggcaa atggtgtctt tgaaggatat ttgctcaatt
     3601 gtaatgacaa cagtaacgtc actattattg gcgtaaacag tgaaagtaat gaatatagca
     3661 atgaatattc cttttcctcc ggtgcggaaa taaatacggg cgtcatatcg gcatgcaccc
     3721 aagaaagtga tttcgttgca ttatcaattc ctgaattaaa ctttgacacg
//
LOCUS       DAOQQN010000324         3746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_11961_length_3746_cov_5.102682, whole genome shotgun sequence.
ACCESSION   DAOQQN010000324 DAOQQN010000000
VERSION     DAOQQN010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11961_length_3746_cov_5.102682"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /gene="rho"
                     /locus_tag="PLG24_10960"
     CDS             complement(<1..1551)
                     /gene="rho"
                     /locus_tag="PLG24_10960"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HMZ25456.1"
                     /translation="MQLDSMLVPELREVAEKNGLTGYKRLSKQELIYKILDQQALTSQ
                     ISEDKLKAMEAESAKSKPHQKEGLRAERRPVNPIKKENQPETASDETIQESKPAIQAG
                     KKPRIKKSTKVELTGTEAEQTPVSVVENEKTETPPEPPRKKFFTTNPESVTEKDAIHV
                     AVKEERKHKTTEPEASSGAKKFQKTEKAPSNQDTSRPNQVNSESETTVAPKQMEKPEP
                     EGPKFNIDFDGMIAGEGILEMMPDGYGFLRSSDYNYLTSPDDIYVSPSQIKLFGLKTG
                     DTVKGMIRPPKEGEKYFALLRVENINGLLPSQIRDRVPFDYLTPLFPIEKFNLTTNPT
                     GRDYGNRMIDLFTPIGKGQRGLIVAQPKTGKTFLLKDVANAIAANHPECFLIVLLIDE
                     RPEEVTDMKRSVNAEVVASTFDEPADNHVKVAGIVLEKAKRLVECGHDVVILLDSITR
                     LARAYNTVAPASGKVLSGGVEANALHKPKKFFGAARKIENGGSLTILATALIDTGSKM
                     DGVIFEEFK"
     gene            2011..3291
                     /gene="serS"
                     /locus_tag="PLG24_10965"
     CDS             2011..3291
                     /gene="serS"
                     /locus_tag="PLG24_10965"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HMZ25457.1"
                     /translation="MLRVEILRNQRDKVVTGLKKRNWSDTDILIIDGIIDLDDKRKEI
                     QTLMDGELAEQNQLAKNIGDLMKNKQAAEAEGLKARVAELKESTKAREENLRDIKQEL
                     EDKLITLPNIPNEDVPFGVGADDNMVYQAWDKPLPVLPEGSLPHWELAEKYNLFDMKL
                     GVKLTGSGFILYRGQGAKLQRALINYFLEYNTAAGYEEILPPHLVNEDSARATTQLPD
                     KEGQMYYCEKDDLYLIPTAEVPVTNIYRDMILSEQEIPGMLTAYTPCFRREAGSYGAH
                     VKGLNRVHQFDKVEIVRVEKPENSYAALDGMIRHVSRLMENLGLPYRILRLCGGDMGF
                     ASAMTYDFEVWSAAQERWLEVSSVSNFETFQSNRLKLRYRDENNNTQLLHTLNGSSLA
                     LARIVAALLENNQSPEGIRIPEVLRNFFGADYIK"
     gene            3305..>3746
                     /locus_tag="PLG24_10970"
     CDS             3305..>3746
                     /locus_tag="PLG24_10970"
                     /inference="COORDINATES: protein motif:HMM:NF016206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HMZ25458.1"
                     /translation="MNSGYFMFMLLGAVGTVVGMILSARLKSKFQHYSQEGLSAGLSG
                     REVAEKMLRDNGITDVQVVSVDGFLSDHYNPQNKTVNLSPDVFNGRSVASAAVAAHEC
                     GHAVQHATSYSMLRFRSAIVPVVQFAATAQQFLLMLALGVMGATG"
BASE COUNT          969 a          794 c          861 g         1122 t
ORIGIN      
        1 tttgaattct tcgaagatca ctccatccat tttggatccc gtatcaatca atgctgtggc
       61 aagaatggtt agtgagccac cgttttctat cttacgggca gcaccgaaaa atttctttgg
      121 cttatgcagg gcattcgctt ccacaccgcc ggataaaacc ttgccactgg ccggtgctac
      181 tgtattatag gcacgggcca atcgggtgat ggagtccagg agaatcacta catcatgacc
      241 acattccaca agtcttttgg ctttctccaa cacgataccg gcgactttaa cgtgattatc
      301 tgcaggttcg tcgaaagtgg aggccaccac ttcagcattg acgcttcttt tcatatctgt
      361 aacctcttca ggtctctcat cgatgagcag cacgatgagg aagcattccg gatgattggc
      421 ggctatggca ttagctacat ccttaagcag gaaagtctta ccggttttcg gctgggcgac
      481 gataagtcct ctttgcccct ttccgattgg tgtaaagagg tcgatcatcc tgtttccata
      541 atccctgccg gtcgggttgg ttgtcaggtt gaatttttca attgggaaga gaggagttag
      601 atagtcaaat ggtaccctgt ccctgatttg acttggcaac agaccattga tattctctac
      661 acgcaataat gcaaaatatt tttcaccttc cttgggaggc cttatcatcc ctttaactgt
      721 atcccctgtc ttgaggccga agagcttgat ctgggaagga gatacataga tatcgtccgg
      781 tgatgtcaga tagttataat ctgaggacct tagaaatccg taaccatcag gcatcatttc
      841 gagaattcct tctccggcta tcatgccatc aaaatctatg ttgaatttgg gaccttccgg
      901 ttccggcttc tccatttgtt taggagccac agtggtctct gattctgagt ttacctgatt
      961 aggcctggaa gtatcctggt ttgaaggcgc tttttccgtt ttttggaatt tttttgctcc
     1021 tgaggatgct tcaggttctg tagttttatg ttttctctct tcttttacgg ctacatgaat
     1081 agcatctttt tctgtaacac tttccggatt ggtagtaaag aacttctttc tgggcggttc
     1141 tggtggcgtt tctgtttttt cattttctac aacactgacc ggagtttgtt ctgcttctgt
     1201 accagtcagt tccactttgg tagatttttt aatccggggt ttctttcctg cctggatagc
     1261 aggctttgat tcctggatag tttcatcaga ggctgtttca ggttgattct cttttttaat
     1321 cggattgacc ggtcttcttt cagcccttag tccttctttt tggtgaggtt tgcttttggc
     1381 gctttcggcc tccatagctt tcagtttgtc ttcggaaatc tggcttgtaa gtgcttgctg
     1441 gtcaagaatc ttatagatga gttcttgctt actgagtctt ttgtagcctg tcaggccgtt
     1501 tttttcagca acctccctta actcgggcac caacatgctg tctaattgca taatgtcata
     1561 catacgtaaa gataaaagtt tttggatttt aacatttaga ggcaggtagc gccgcttaat
     1621 gttaccggat ttgactaccg gattaatttg ataatttaat tcaattaaaa tttctttcaa
     1681 atacaggcgg atcggtacag ggatgagacc tttagcaacg aacaatgtcg atactaagat
     1741 tcattgccag tactggaact ttctatattt ttaagatttt cagattatta caacactcat
     1801 ccttgaaaca atgactgccc atttaaaatg accgatatac gtagtattag attcgggaaa
     1861 ttaagcggta aatcaatatt gctacagtga tgggcgtctt aaagctcttt cagcttgaaa
     1921 tgatatgcaa atgtacatat ttttttttat tcaactatat ttgtgcctta attttaaatt
     1981 taatataaat atttattcaa atccttctct atgttacgcg ttgaaatact tagaaatcaa
     2041 agagataaag ttgttaccgg ccttaaaaaa aggaattggt cagatactga tattctcata
     2101 attgatggca tcattgacct tgatgataaa agaaaagaaa tccagacgct gatggatggt
     2161 gaattggctg aacaaaacca gttggcaaaa aatattggcg acctgatgaa gaataagcag
     2221 gctgcagaag cagagggatt gaaagcacgt gtagcagagc tgaaggagtc taccaaggcc
     2281 agagaagaaa atttgcggga tatcaagcag gaattggaag ataagctcat taccttaccc
     2341 aatattccga atgaggacgt ccccttcggt gtcggagcag atgacaatat ggtctatcag
     2401 gcatgggaca agccgttgcc tgtattgccc gaagggtccc tgccgcattg ggaattggct
     2461 gaaaaatata acctttttga tatgaagctc ggggtaaaac tgacgggaag tggcttcata
     2521 ttgtatcgtg gacaaggagc aaaacttcag cgggctctga tcaattattt cctggaatac
     2581 aatacagccg caggatacga agaaattttg cctccgcatc tggtcaatga agactctgcc
     2641 agagcaacaa cccaacttcc cgataaggaa ggccagatgt attattgcga aaaggatgac
     2701 ttgtacttga tcccaacagc tgaagtgccg gtcaccaata tttatagaga tatgatcttg
     2761 tcagaacagg aaattccggg gatgctgacc gcttatactc cttgttttag aagagaagca
     2821 ggttcttatg gcgcacacgt caagggactc aaccgggttc atcagttcga caaggtggag
     2881 attgttcggg tagaaaaacc ggagaactca tacgctgccc tcgatggcat gattcgccat
     2941 gtatcccgac tgatggaaaa cctgggcttg ccttatagga ttcttcgcct atgcggaggt
     3001 gatatgggct tcgcctctgc catgacctat gattttgagg tttggtcggc tgcacaggag
     3061 cgatggctgg aggtcagctc tgtatccaac ttcgagacgt tccaatccaa caggttgaaa
     3121 ctacgttatc gggatgaaaa caataataca caactcttac acacactcaa tggaagctca
     3181 ctggcactgg cccgcatcgt cgctgcactc ctggaaaaca atcaatctcc tgaaggaatc
     3241 agaattcctg aagtgctgcg gaactttttt ggggcagatt acattaaatg aaaaaaaata
     3301 ttgaatgaat agcggttatt tcatgtttat gctcctcggg gcagtgggaa ctgttgtggg
     3361 catgattttg agtgcaagat taaagtcaaa atttcaacac tatagtcagg aaggactcag
     3421 cgctggcctt tccggtcggg aagtcgccga gaagatgttg cgcgacaatg gtattactga
     3481 tgtacaggta gtctccgtcg atggcttcct gtccgaccac tataacccgc agaacaaaac
     3541 ggtcaatctc agcccggacg tattcaatgg caggagcgta gcttctgcag ctgtggctgc
     3601 acacgagtgt ggtcatgccg ttcagcatgc cacatcgtat tccatgttgc gctttaggtc
     3661 ggcaatagtt ccggttgtac aattcgcggc caccgcccag caatttctac tgatgctggc
     3721 actgggtgtc atgggagcta ccggta
//
LOCUS       DAOQQN010000325         3742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_11976_length_3742_cov_5.779224, whole genome shotgun sequence.
ACCESSION   DAOQQN010000325 DAOQQN010000000
VERSION     DAOQQN010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11976_length_3742_cov_5.779224"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..263
                     /locus_tag="PLG24_10975"
     CDS             66..263
                     /locus_tag="PLG24_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HMZ25459.1"
                     /translation="MKFSTYLTKIDGVSIYPVISLVLFVLFFTIVILWAYRSDSKQIE
                     HFENLPLEEGENSGSNPLNSI"
     gene            263..1402
                     /locus_tag="PLG24_10980"
     CDS             263..1402
                     /locus_tag="PLG24_10980"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ25460.1"
                     /translation="MKRYIFSTILILICNLAIPLQSFSQTPATGDNNSFSFSNLSTFD
                     WALLAFAATLLLIIFILTDTLRLAMNNRKDSEFSGKSGSKTIPVILLILGFTGVVTFD
                     AKAAAPSFDGIMSPAHMILYSVILIELIAIYVLINWIKYFSGIQSYKERIEEASEVKS
                     KPTTSLWARLNKLRPIEEEGTLDIGHNYDGIRELDNATPPWFTISFLATIVFSAIYLY
                     RYHIAYSAPNQIQEYKTEVALAQAAQKEFLASQGNLVDENTVTMLDEAGINSGKALYD
                     ANCVACHGSKGEGGVGPNLTDDYWLHGGSIKDVFKTIKYGVIEKGMKAWQEDFSANQI
                     AQISSYIESIHGTNPPNAKEKQGELYEVKKNDATSSEEKPAEVSN"
     gene            1496..2905
                     /gene="ccoG"
                     /locus_tag="PLG24_10985"
     CDS             1496..2905
                     /gene="ccoG"
                     /locus_tag="PLG24_10985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HMZ25461.1"
                     /translation="MIEDSIYDTGNEAFRDRMSNTTAEGKRKWFYVSKPPKGKYTKWR
                     NRLNFFYFALFFTLPFLRYQGRPFFMINFPKGEFIVFGKIFWPQDFFLFAVGMITMIV
                     FVILFTVIWGRLFCGWICPQTVFMEGLFRKIEWWIEGSPSQQKKLDQMPWNWEKIRKK
                     GLKQLIFLSVSFLIANTFLAYIISTDELFTIIREPIAEHLTLLTGIIGFTLVFYFVFA
                     YVREIVCTTICPYGRLQSVLVDQDSMQVSYDYVRGEPRGKMSKKVENNFGDCIDCKKC
                     VIVCPTGIDIRDGVQMECVGCTACIDACDEVMDKISRPRGLIRYASERGIRENKKFVF
                     TTRMKAYSGALVILLGIMTYLIASSKAISANISRVKGQLFQELPDNKISNLYDAKILN
                     KKNKEYPVELKIIGMEGQVKMINNHPILKKQGLTDLTFFVILDQNKVQKRSNKIKIGV
                     FADGELVDEINTTFLGPFK"
     gene            2911..3345
                     /locus_tag="PLG24_10990"
     CDS             2911..3345
                     /locus_tag="PLG24_10990"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HMZ25462.1"
                     /translation="MNWGHGILITIIIFVASILGMVVLSSRQSIDMMDEGYYEKELKH
                     QELIDESANFARDNGKVEINDLGKYLEVKIPENLTNDITSGNILMMRPSDKSLDVNIP
                     MQMKSGKQLIDKNLLIKGLYNIRMSWINNGTTYYQTFTYLVS"
     gene            3345..>3742
                     /locus_tag="PLG24_10995"
     CDS             3345..>3742
                     /locus_tag="PLG24_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HMZ25463.1"
                     /translation="MLEWILTGLIIGLAGGMHCIAMCGPLAISMPWSSGPRFFLKTII
                     YHLGRTTTYVLLGLAFGLIGNALFIAGYQQWLSIIAGLFVLYFALKKIFPGKVPDINL
                     PGSAKLTKVITRMISNKQIVNNPYLFGMVNG"
BASE COUNT         1189 a          653 c          773 g         1127 t
ORIGIN      
        1 atcattgctt tgattgctta cttgcagagg ttgggaacag atgctaaaac aataaataat
       61 gcggaatgaa attctcaacc tatttgacaa aaattgatgg agtatccatt tatccggtga
      121 tctcactcgt gctttttgta ctcttcttta ccatagtcat tctttgggct tatcgttcag
      181 atagtaagca aattgagcat tttgaaaatt taccactgga agaaggagaa aattccggtt
      241 ctaacccttt aaattcaata tgatgaaacg atatattttt tcgaccattt taatcctgat
      301 ttgcaatcta gcaattccat tacaaagctt tagtcagaca cctgcgacag gtgataataa
      361 tagcttttca ttctctaatc ttagtacttt cgattgggcc ttgttggcat ttgcggcgac
      421 cttattgctc atcatcttca ttttaacaga tactttaaga ctggcgatga ataaccgaaa
      481 ggattccgag ttttccggaa aatcagggag taagacaatc ccggtaattc ttctcattct
      541 tggatttacc ggtgtggtga cattcgatgc gaaagctgct gctccttctt ttgatgggat
      601 tatgagtcct gctcacatga ttctctatag tgtcattcta atcgaactga tagcaattta
      661 tgtattaatt aactggatta agtacttttc cggtattcag tcctataagg aaagaattga
      721 agaagcaagt gaagtcaaaa gtaaacctac taccagtctt tgggcacgat tgaataagct
      781 tcgacctata gaagaagaag gcaccctgga tatcggacat aactatgacg gaattcgtga
      841 gttggacaat gccactcccc catggtttac aataagcttt ttggcaacca tagtcttttc
      901 tgctatttat ttgtatcgat atcatatagc ttactctgca ccgaaccaaa tccaggagta
      961 caaaactgaa gttgctttgg ctcaggcagc acaaaaggaa tttcttgcaa gtcaaggtaa
     1021 ccttgtagat gaaaatacgg taaccatgct ggatgaagcc gggatcaatt cggggaaagc
     1081 cctttatgac gcaaattgtg tggcctgcca tggctccaaa ggagaaggtg gtgtaggacc
     1141 aaacttaaca gatgattatt ggttgcatgg tggaagtatc aaggatgtat ttaaaacgat
     1201 taaatatggt gtaattgaga aaggaatgaa agcctggcaa gaggactttt ccgccaatca
     1261 aatagcacaa atatccagct atatagaatc aatccatgga acaaatcctc caaatgccaa
     1321 agaaaaacaa ggcgagttat acgaagttaa gaaaaatgac gctacatctt ctgaggaaaa
     1381 accggcggaa gtatcaaatt aataaattac gatatggaac agccgtctgt cttatcactg
     1441 attggctgtt ccatttattt tagaggagtt ttgttgcatt attaattcat tgatcatgat
     1501 agaagatagt atatatgaca ccggcaatga ggcttttcgc gacaggatga gtaacacaac
     1561 tgccgaagga aagcgcaaat ggttttatgt tagcaaacca cctaaaggca agtacacaaa
     1621 atggcggaat cggttgaact tcttctattt tgctttattt tttactttgc ccttccttag
     1681 gtatcaaggc agaccgtttt tcatgataaa ttttccaaaa ggtgaattca tcgttttcgg
     1741 gaaaatattc tggccacagg atttctttct atttgcagta ggaatgataa ccatgatagt
     1801 ctttgtgatt ctatttacag taatctgggg tagattattc tgcggctgga tttgtcctca
     1861 gactgtattc atggaaggct tgttcaggaa aattgagtgg tggatcgaag gtagtccatc
     1921 gcaacaaaag aaactggatc aaatgccctg gaattgggaa aaaattcgaa aaaaaggcct
     1981 gaagcaactt atcttcttaa gtgtatcttt tttgatagca aatacttttt tggcttatat
     2041 tatcagtacg gatgaattat ttaccattat ccgtgagcca atagcagaac atttaacctt
     2101 acttaccgga attattggtt ttacattggt tttttatttc gtttttgctt atgtgaggga
     2161 aattgtttgt acaacgatat gtccttacgg caggttacaa agcgtattgg tggaccagga
     2221 ctccatgcag gtttcctatg attatgtacg aggagaacct agaggtaaga tgtcaaaaaa
     2281 agttgagaat aattttggcg attgtattga ttgcaagaaa tgtgttatcg tttgtcctac
     2341 gggaatcgat atacgcgatg gcgtacaaat ggaatgtgtg ggttgtacag cttgtatcga
     2401 tgcatgcgat gaggtaatgg ataagattag cagaccacga gggctcatca gatatgcatc
     2461 cgaacgagga attcgagaaa ataaaaaatt tgtctttaca accagaatga aagcatattc
     2521 gggagctctt gttattcttt taggaataat gacgtatctg attgcatctt ccaaagcaat
     2581 ttctgccaac atctcaaggg taaaaggaca acttttccag gaactgcctg ataacaagat
     2641 cagtaacctc tacgatgcca agattcttaa caaaaagaat aaggaatatc cggtcgaact
     2701 aaaaattatt ggcatggaag gccaggtcaa aatgatcaat aatcatccga tactcaaaaa
     2761 acaaggattg accgatttga cttttttcgt tattcttgat caaaacaagg tgcaaaaaag
     2821 aagcaacaag ataaaaattg gagtttttgc agatggagaa ctggttgatg aaattaatac
     2881 aacattttta ggaccattca aataaaacat atgaactggg gacacggtat tttaatcaca
     2941 ataataatct ttgtagcatc cattttaggc atggttgtat tgtcatccag acaaagcatt
     3001 gacatgatgg atgaaggtta ttatgaaaag gaactgaaac atcaggaatt gatcgatgaa
     3061 tcagcaaatt tcgcacgtga taatggcaaa gttgaaatta atgatctggg aaaatatctt
     3121 gaggttaaaa tacccgaaaa tctaacaaac gatatcactt ccgggaatat tctaatgatg
     3181 cgaccttcag ataaaagtct tgatgtcaat attccaatgc aaatgaagtc cggaaagcaa
     3241 ttaatagata aaaatttact aataaaaggc ctttacaata tccggatgag ctggattaat
     3301 aatggcacaa cgtattatca gacgtttacc tacctggtat cctgatgctt gaatggatac
     3361 tgaccggatt gatcataggc ttagccgggg gtatgcactg tattgccatg tgcgggcctt
     3421 tagccatctc tatgccttgg tcgtcaggac cgaggttctt cttgaagaca atcatatatc
     3481 atttaggccg tactactact tatgtactcc tgggattggc attcggactt attggcaatg
     3541 ctttatttat tgcaggttat cagcaatggt tgtcaataat agcaggtctg tttgttttat
     3601 attttgcctt aaaaaagata tttccaggga aggtaccaga tatcaatttg cccggcagcg
     3661 ctaagctcac taaagtcatc acccgaatga tcagcaacaa gcaaatcgtg aataacccat
     3721 acctttttgg catggtaaac gg
//
LOCUS       DAOQQN010000326         3739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_11992_length_3739_cov_5.865635, whole genome shotgun sequence.
ACCESSION   DAOQQN010000326 DAOQQN010000000
VERSION     DAOQQN010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11992_length_3739_cov_5.865635"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PLG24_11000"
     CDS             <1..319
                     /locus_tag="PLG24_11000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ25464.1"
                     /translation="VVSPECYIDTYLSSSIFNRPAQLSLYPNPARNVFSFQINPGGQG
                     VAFITLTDTSGRTIKQWKMNYVNGENNLTFGITNVEAGIYMVQMTLSESRRRYTGKLV
                     VID"
     gene            complement(399..2201)
                     /locus_tag="PLG24_11005"
     CDS             complement(399..2201)
                     /locus_tag="PLG24_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ25465.1"
                     /translation="MKQLISIFIFLLASMTISAQSLELARQYFEDGEYEKAAAIYKSL
                     WNKNKGNTTYLDALTDCYMALKDYDTAASVMSSAIEQNPTMLNLRVSYASLLNTLNKP
                     KEAEAQYNYVMKNIPANPTVILLTSRALEKAGKPELAIQVLEKGRDLVPNANYFTYDL
                     SQLYLKTGQQEKMLAIAVDQLIADPSYYSLFTTSIQRSFQSDSEYDNLLKAVFNRAQQ
                     YPDNSQLIELMSWIYMQKRDFANAMRQLKALDIRTGNDYTRVYSLARTAFNEKQYDVA
                     VSGFDYILEKGRANPYFYEAARFRLQALVAKSVVNTIISPSEQEVIDKDYTDYINTYS
                     ETGRAVYLSYDYAEFLMQYRDNLPKAIEILQTIVDNGTYSPPVIAEVKIRLADYLLIE
                     GKRWDASLLYSQVDKDFKEGPLGQEARYKNALLSYYVGDFEWSQAQFDALKTATSRLI
                     SNDAIDRSVFIMDNMGMDSTDLAMKKYAATELLIYQNKLSEALVKLDSILIDFPGHAL
                     DDDVLYAKAHIFLKMKDYNKAIDCYEKVFTKYKDEIRADNSIYELAQLYDYTLNDKEK
                     AKALYERLFNEYTGSVFAFDARQRFRVLRGDVDQ"
     gene            2405..3280
                     /locus_tag="PLG24_11010"
     CDS             2405..3280
                     /locus_tag="PLG24_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HMZ25466.1"
                     /translation="MIFSMTGFGASKKAFYDKILSVEIKSVNSKIIDIRIRSPFNLRE
                     KENILRKEVADTALRGKIEVTVELSSANSTPLGMINEELLKSYAMSIKPIAADLELDT
                     SGLIAGLLRIPEVLAGGNNELSEEDWLILEATLDDALDRFQAYKQEEGKAIENDLLAC
                     INTIEESLVKIRDFETERIDRIRERITNGLEEFMPTESIDKNRFEQELIYYIEKIDFS
                     EEKSRLSQHCKYFRETLELDEVSKGRTLNFISQEMGREINTLGAKAYQSDIQRLIVVM
                     KDELEKIKEQTANIV"
     gene            3284..>3739
                     /gene="gmk"
                     /locus_tag="PLG24_11015"
     CDS             3284..>3739
                     /gene="gmk"
                     /locus_tag="PLG24_11015"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HMZ25467.1"
                     /translation="MEFEGKMIVVTAPSGAGKTTIVKYLLEKMDNLDFSISATTRDKR
                     PYEVDGKDYFFITKEQFEELIAKKAFAEYEIVYEGQYYGTLAAEIERIWQNGKHIIFD
                     IDVQGALTLKRKYMESCLTIFIRPPSKELLIERLTNRQTESPEALKKRID"
BASE COUNT         1110 a          807 c          813 g         1009 t
ORIGIN      
        1 tgtagtgagc ccggaatgtt atatcgacac atacctttcc tcatcaatat tcaatcgtcc
       61 tgcccaactc agcctgtacc ccaatccggc acgtaatgta ttttccttcc agataaatcc
      121 gggaggacaa ggtgttgcat tcatcacact aacggacact tccggcagaa caatcaagca
      181 atggaaaatg aattatgtca atggtgagaa taatctaact tttggtatta ccaacgtaga
      241 ggcaggtatt tatatggtcc agatgacgct gagcgaaagc agacgaaggt atacgggcaa
      301 gctcgtggtg atagattgac ccttcctgta actgttttca gccgaccgta gcatagcaga
      361 gcctgtccct gaggaaattc tttttccagg gacaggcttt attgatctac atctcctcgc
      421 aaaacccgaa atcgttgtct tgcatcaaag gcaaaaacgc tcccggtgta ttcattgaac
      481 aatcgttcat acaacgcttt cgccttttcc ttatcattga gggtataatc atacaattga
      541 gctaattcat aaatagaatt gtctgcccga atttcatcct tatatttagt aaatactttt
      601 tcgtagcaat caatggcctt gttataatct ttcattttca gaaatatgtg cgccttggcg
      661 tacaacacat catcatcaag tgcgtgaccg ggaaaatcta tcaatatgga atccagttta
      721 accaatgcct ctgataattt attctgataa atcagcaatt cggtggcagc atatttcttc
      781 atcgccaaat cggtcgaatc catgcccata ttatccatga taaatacact cctgtcgatg
      841 gcgtcattgg atattaacct cgatgtagca gttttaagag catcaaactg cgcctgagac
      901 cattcaaaat cacccacata ataagaaaga agagcatttt tataccttgc ctcctggcct
      961 aagggacctt ccttgaagtc tttatccacc tgactataga gtaaagaagc atcccagcgc
     1021 tttccttcga tcaaaaggta atcagccagt cggattttta cctctgcaat cacaggaggg
     1081 gaatatgtgc cattatcaac gatggtttgt aaaatttcaa ttgctttggg aagattgtcc
     1141 ctgtattgca tcaaaaattc agcataatca tatgataggt acacagccct gccggtctcg
     1201 gaataagtat tgatatagtc ggtataatcc ttatctatca cttcctgttc gctcggagaa
     1261 ataatggtgt tgaccacact tttagctacc aaagcttgca agcggaaacg cgccgcttca
     1321 taaaaatagg gattggccct gcctttttcc aaaatataat cgaagcccga cacggcgaca
     1381 tcgtattgtt tctcgttgaa agccgttctc gccaatgagt atacccgggt gtagtcattg
     1441 ccggtacgta tatccaacgc cttgagctgt cgcatcgcat tagcgaaatc ccgcttttgc
     1501 atataaatcc acgacatcaa ttctatcaac tgtgaattgt ccgggtactg ctgcgctcgg
     1561 ttaaaaaccg ccttaagcag gttgtcatac tccgaatcac tctggaagct tcgctggatg
     1621 gaagttgtaa acaggctgta ataacttggg tctgctatca actgatctac tgcgatggca
     1681 agcatttttt cctgttgtcc tgtcttcaaa tacagttggc tcaggtcata ggtaaaataa
     1741 ttagcattgg ggacaagatc ccggcctttt tccaacacct ggatcgccaa ttccggctta
     1801 ccggcctttt ccagggctct ggatgtaagc aaaattactg ttggattagc gggtatattc
     1861 ttcattacgt aattatattg tgcttcggct tccttgggct tgttgagggt attgagcagg
     1921 gaagcatagg acacacggag gttgagcatg gtggggttct gctcaatggc actcgacatc
     1981 accgatgcag cagtatcata atccttgagg gccatgtagc aatcagtcag cgcatccagg
     2041 taggtagtgt tacccttgtt tttattccaa agggatttgt agattgctgc agctttttca
     2101 tactcaccgt cttcaaagta ttggcgtgca agctcaaggc tctgtgccga tattgtcatg
     2161 gaagcgagca gaaatatgaa aatagaaatg agttgtttca tgatgaaatt taatgggcaa
     2221 atttacagat tcgccatggt tgtattgaca attgccgaag taaataaaac gaaaagggac
     2281 gcgaattgtt ttatccggct ctcttcattc ataaaattaa ctaagtaagc ctttgcaatg
     2341 ctttttgtgc ctagatttgc ccaaatttta ctatttttgc gtttctcaaa aaatcgcata
     2401 tcctatgata ttttcaatga ccggttttgg tgcatccaaa aaagccttct acgataaaat
     2461 tctatcggtt gaaatcaaat cggtcaactc aaaaataatc gatattcgca tccgttctcc
     2521 cttcaacctt cgggaaaagg aaaatatcct ccgcaaagaa gtggcagaca cagcactacg
     2581 tggcaagatc gaagtgaccg ttgaattatc atccgccaac agtacgccgc ttggaatgat
     2641 caacgaagag ttgctcaaga gttatgctat gtccatcaaa cccatcgccg ctgatctgga
     2701 attagacaca tccggcctca tcgccggatt gctccgtatt ccggaggtat tggcaggagg
     2761 caacaacgaa ctgagtgaag aagattggct tatattggag gccactctgg atgatgctct
     2821 tgacaggttt caggcatata agcaggagga gggtaaggcc atcgaaaatg atctattggc
     2881 ttgcattaac accattgaag agagtcttgt gaagatccgg gattttgaga ccgaacggat
     2941 agacagaatc cgggaacgga tcaccaatgg tctcgaagaa tttatgccga cagaaagtat
     3001 cgacaagaat aggtttgagc aggaattgat ctattatatt gagaaaattg atttcagtga
     3061 agaaaaaagc agactttcgc aacattgtaa atatttccgg gagaccttag aactggacga
     3121 ggtgagcaag gggcggacac tcaattttat cagtcaggag atgggccgcg aaatcaatac
     3181 tttaggagcc aaggcctatc aaagcgatat ccagcgcctc atcgtagtca tgaaggatga
     3241 gctggaaaaa atcaaggaac aaactgccaa tattgtctga tccatggagt tcgaaggaaa
     3301 aatgatcgtg gtaacagccc cttcaggcgc cggaaagacg accatagtca agtatctgct
     3361 cgagaaaatg gacaatctgg acttttccat ttcggcaacc acgagggaca aaaggccata
     3421 cgaagtagat ggaaaagatt atttcttcat tacaaaggaa cagtttgagg aattgattgc
     3481 caaaaaagca tttgcggaat atgaaatagt gtacgaaggc cagtattatg gcacgctggc
     3541 tgccgagata gaacgtatat ggcagaacgg caagcacatc atattcgaca tcgatgtaca
     3601 gggcgcactg acactcaaaa gaaaatatat ggagagctgc ctcaccatct tcatccgacc
     3661 gccaagtaag gaattactta tagaaaggct caccaacagg cagacagaaa gccctgaagc
     3721 actgaaaaaa aggatcgac
//
LOCUS       DAOQQN010000327         3730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_12048_length_3730_cov_4.397279, whole genome shotgun sequence.
ACCESSION   DAOQQN010000327 DAOQQN010000000
VERSION     DAOQQN010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12048_length_3730_cov_4.397279"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLG24_11020"
     CDS             <1..199
                     /locus_tag="PLG24_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25468.1"
                     /translation="TADMREDISEFAEEAKETLAEWKEKAAEKTADMREDIEEFATEA
                     KEKIETTSKSFFSKIKSFFGK"
     gene            complement(318..1529)
                     /locus_tag="PLG24_11025"
     CDS             complement(318..1529)
                     /locus_tag="PLG24_11025"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMZ25469.1"
                     /translation="MGKPVVYHLADRIVKMVESATLQMTAKARKLKQEGKDVINLSIG
                     EPDFDTPQFIKDAAKVALDQGYTKYTPVSGLIELKRSIQEKFKRDNNIEYALDEIVVS
                     NGAKQSIANVAMSVLNEGDEVIIFAPYWVSYLEIVHLAGGVPVIVHAGIENDFKVLPS
                     QLEAAITPKTRMVIFSSPCNPTGSVYSMEELQAFAEILGTKDEIYIVSDEIYEYINYT
                     GNHASIASIPSVKDQTITINGFSKGFAMTGWRLGYMGAPREVAKATDKMQGQFTSGAA
                     SFSQKAASLALETDLTETYKMRDAFLKRRDLILNLLKEIPGIKCNVPEGAFYVFPNVS
                     AYYGKKTPDGTVIENGDHLCTYILDTAYVALVAGSAFGDEECIRISYAASDEEIIEAC
                     KRIKEALSHLN"
     gene            1690..3534
                     /locus_tag="PLG24_11030"
     CDS             1690..3534
                     /locus_tag="PLG24_11030"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HMZ25470.1"
                     /translation="MITIHELPALRILIGLVAGILLYPGYGMLSGWTIIALIVVTLLI
                     ILAVYFLRVRYKIRYLPSLILIIPCVTIGILLTFFTDERNSLTHMNHFPEMAKKKSRI
                     IAECLEAPKKGKSWQVLLKAEAIEDSLGEYRQLDGKFIIFSKSFERTPEPGDLVSVIA
                     QLDEIRRNEVPYGFDYQKYLASKHIYKIAYIKDADLQIIKTSPIYHLKFMAYKVRNWC
                     IERCKAILGRNEMADLASGLIFGYREEIDESTQKAFVETGSVHLLAVSGMHVALIYIN
                     ILFFLKVIGFRWLVGEKKMILTAVMFIWAFTFIAGLSASIVRAALMLTILVVGKLFGR
                     KGTGLNAVFSGAVLMITSQPFVIYDVGFQLSFSAVIGIILLQHKIKQTLPAPLSGQKT
                     LASLISVTLAAQAGTLPLILYHFHQFPVYFLISGLVAVFISDWVIKIGLAVILVSTFS
                     MPLAIYFGPLWEISTTALLKSVEWISKIPFGIVTKIYFSLSMALVLSVMLLLTYIHIQ
                     NRLRNYRTLMILGILLLFALEGWSLFNAAHSESTVRIQLQQKQVVIYRKGLIGTVAAD
                     YRLDSIFVMNKISGYITATRIKDIKYQALTENEIDNIPGEVKADLSLH"
BASE COUNT         1080 a          686 c          756 g         1208 t
ORIGIN      
        1 aacagcagac atgcgtgaag atatttctga attcgcggag gaggccaagg agactttggc
       61 tgaatggaag gaaaaagctg cagaaaaaac cgcagatatg cgtgaagata ttgaggaatt
      121 tgcaactgaa gccaaagaga aaatcgaaac tacatcaaag tctttctttt cgaaaatcaa
      181 aagttttttc ggtaagtaac aggcataaat tagtgggcgg tagcttcata caaagataat
      241 gtatgaattt tttcctgtta ttcctttaaa cattgctgaa taaagaaaag cccggtaaat
      301 gccgggcttt tctttattca attcaggtga ctcaaagcct ctttaattct cttgcaagct
      361 tcgataattt cttcatctga agcagcatag gatattctga tacattcttc gtcaccgaaa
      421 gctgaaccgg caacaagagc cacataagct gtatccaaaa tatatgtgca caaatgatct
      481 ccattttcta tgactgtccc atcaggggtt ttcttgccat aataggcgct cacatttggg
      541 aagacgtaga atgctccttc cggaacattg cactttattc ccggtatttc tttcaacaga
      601 tttaatataa ggtcccttct tttgagaaaa gcatctctca ttttataggt ttctgtcaga
      661 tccgtctcca gagcaagtga agccgctttc tgactgaagg aagccgcgcc tgaagtgaac
      721 tgcccctgca tcttatcagt cgcttttgca acttctcttg gtgcacccat atagcctaac
      781 ctccaaccgg tcatagcaaa accctttgaa aatccgttga ttgttatggt ttgatccttg
      841 acagatggga tggaagctat gctggcatga ttgccggtat agttgatgta ttcatatatt
      901 tcatctgaga cgatgtagat ttcatctttt gtaccaagaa tttccgcaaa tgcctgaagt
      961 tcctccatcg agtatacgct gcctgttgga ttacatggac ttgaaaaaat taccattctt
     1021 gtcttaggtg taatagccgc ttccagctgg gaaggcaata ctttaaaatc attttcgata
     1081 cctgcatgga caattacggg aactccaccc gccaaatgta caatttcaag ataagaaacc
     1141 caataaggcg cgaaaatgat cacttcatcg ccttcattga gaacactcat cgcaacattc
     1201 gcgatgcttt gcttggctcc gtttgatact acgatttcat caagtgcata ctcaatgtta
     1261 ttgtctcgtt tgaacttttc ctgaatagac ctttttaact caataagccc cgaaacagga
     1321 gtgtatttag tatagccctg atccagagct actttagcag cgtctttaat aaattgcggt
     1381 gtatcaaaat caggctcacc gatgctcaga ttaataacat ccttgccttc ttgctttaac
     1441 tttctggctt tagccgtcat ctggagtgtt gcggactcga ccattttgac gattcggtca
     1501 gccagatgat aaactacagg ttttcccatt actaaattat tttccgacaa aggtaatgag
     1561 tatttgtatt ataatccaca ggacacccct atcaaaaaat atttgttaga tcttgatata
     1621 attcaataaa aatcttttgt aaataattcg gtatttatta tttttacggt aataaaatct
     1681 tcagtcacca tgatcaccat tcacgaacta ccggcattac gtattttaat tgggttggtt
     1741 gcaggcatcc tgctatatcc cggatatggt atgttatccg gatggacaat aattgccttg
     1801 attgtagtaa cccttcttat cattttggcc gtttatttct tgcgggtacg gtataaaatt
     1861 cgatatctgc catcacttat tctgatcatt ccatgtgtca caattgggat tctattgaca
     1921 ttctttactg atgaaagaaa ttctttgacg cacatgaatc attttccgga aatggccaaa
     1981 aagaaatcca gaataatcgc tgaatgtttg gaggccccga aaaaaggaaa gagctggcaa
     2041 gttttgttaa aagctgaagc aattgaagat tcactgggtg aataccgtca attggatggg
     2101 aaatttatta ttttttcaaa atcatttgaa agaactccgg aacccggaga cttggtttcg
     2161 gttattgcac agttggatga aatacgtcgt aatgaagtcc cttatgggtt tgactatcag
     2221 aaatatttgg cgagtaaaca tatatataaa attgcatata taaaggatgc agatttgcag
     2281 atcattaaaa cttcgcctat ttatcatttg aaattcatgg cttataaggt tcgaaactgg
     2341 tgtattgaac gatgtaaggc tatactagga aggaatgaaa tggctgatct cgcttcaggt
     2401 ttgatatttg gatacaggga agaaattgat gagtccaccc agaaggcatt tgttgaaaca
     2461 ggttctgttc atctattagc agtatccggt atgcatgttg cgctgatata tataaatatt
     2521 ttgtttttcc ttaaggtaat tggttttagg tggctggtgg gggagaagaa gatgatatta
     2581 acggcagtta tgtttatatg ggcatttaca tttattgcag ggttatcagc atctatagta
     2641 agggcagctc tcatgcttac tattttggtt gtggggaaac ttttcgggcg aaaggggacc
     2701 ggtcttaatg ccgttttttc cggagccgtg ttaatgatta cttctcaacc ttttgttatc
     2761 tatgatgttg gttttcaatt atcattttca gcagttattg gaattatcct gcttcaacac
     2821 aagattaagc aaacattgcc tgcacctttg tccgggcaaa agactttagc aagcctcatt
     2881 tccgtcacac tggcagcaca agcagggaca cttcctctca ttctgtacca ctttcatcag
     2941 tttccggtct attttctgat ttccggtctc gttgctgtct ttatttcaga ttgggtgata
     3001 aaaattggat tggctgtaat tttggtctct actttcagta tgccattggc tatttatttc
     3061 ggtccattgt gggaaatatc tacgacagca ttattaaaga gtgtagaatg gataagcaag
     3121 attccttttg gaatagtgac taaaatttat ttttccttga gtatggcttt ggttctgtcg
     3181 gtaatgctat tactgaccta tattcatatt caaaatagat tgagaaatta tagaactttg
     3241 atgatattag gcatattatt attgtttgct ttggagggtt ggtcgctttt caatgctgca
     3301 cacagtgaaa gtacagtgag aattcagttg cagcaaaagc aggttgttat ctaccgtaaa
     3361 gggttgatag gaacggttgc agcagactac aggctggatt caatttttgt catgaataaa
     3421 atttccggat atattactgc tactcggatt aaagatatta aatatcaagc gcttacagaa
     3481 aatgaaatag acaatattcc cggggaggta aaggcggact tgtccctaca ttaaattatt
     3541 atgcttaagt gaccttgctt ccatttaaaa caattgtaca tttgtgtttt ataaacaaaa
     3601 tagactggca tgttttggac gcaggaaata ttgaaagcta ttgataaagg tcagccccaa
     3661 ttgataaatg actcgaagac tccatcaggt gaaccgcatg ttggttcatt aaggggagtg
     3721 ttgatacatg
//
LOCUS       DAOQQN010000328         3717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_12099_length_3717_cov_6.166849, whole genome shotgun sequence.
ACCESSION   DAOQQN010000328 DAOQQN010000000
VERSION     DAOQQN010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12099_length_3717_cov_6.166849"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="PLG24_11035"
     CDS             complement(<1..1155)
                     /locus_tag="PLG24_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HMZ25471.1"
                     /translation="MSKNIAFLFGLAILSVFVASCGRSSKMDGQLIGVSKRPSWKGIN
                     PYGMIYVRSGTLTIGSDDQDLSSTYISRPKSISIAGFFMDETEITNNEYRQFVNWVRD
                     SIAHTILGDVEEDENGKSHILWDNEIDYTDEALADMFYSGDDILNGKKEFDVDKLVFN
                     YEWMDWQKAAADRGKSTRKTYIKKEKTKIYPDTMVWIRDFAYSYNEPMTRNYFWHPAY
                     DDYPVVGVNWKAATAFCAWRTKIWDMYRPQDVHTEGFRLPTEFEWEYAARAGHNSAPY
                     PWGAYGLRNSKGCLLANFKPRRGNYPEDGGLWTVKSNAYFANDYGFYCMAGNVSEWTS
                     TAYAENAYSFIHDLNPDIRYDAKDEDPEGLKRKVIKGGSWKDIGYFLQNGT"
     gene            complement(1413..2381)
                     /locus_tag="PLG24_11040"
     CDS             complement(1413..2381)
                     /locus_tag="PLG24_11040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMZ25472.1"
                     /translation="MSKFHFLTGVLLMLTIALAHKVNAQQIAQHGLYMLEPYWYNPAF
                     GGLNNALTITGNFRRQWAELPGRPSTQYISAHMPLRTINSGVGLNFKNDELGATRISA
                     ATMSYNYILPLRVFVSVGVGLGLNTSSINGDLLRTPEGNYENGINHNDPNIPAGKSSS
                     TNGTLNLGIVSRFNAIELGLSAIQTTSIGKRNSDLYYYKPYTHLILYTAYYYQIDDNW
                     KILPSALLKYDLATIQTELDILAYNKNIFGGIGIRGYDNSSIDAVKVMLGGRITERIL
                     VAYNFEGNISKERAYIGNTHELLVQFRIKTNFKTTKREKIIYHPRI"
     gene            complement(2563..2766)
                     /locus_tag="PLG24_11045"
     CDS             complement(2563..2766)
                     /locus_tag="PLG24_11045"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HMZ25473.1"
                     /translation="MPVVKVIEVIASSEKSFDDAVQNAVKEISKTIKNIDSVYVKDFK
                     CHVQDNKIISYGVICKVSFRVES"
     gene            3060..>3717
                     /locus_tag="PLG24_11050"
     CDS             3060..>3717
                     /locus_tag="PLG24_11050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ25474.1"
                     /translation="MEIKEKLWTRNFINVSVSNFLIACSFFLLMPTIPVYLAEQLRVP
                     HSQIGIVLSSYAIALLIIRPFSGYLVDIYSRKPLYIIGIAFFTLIFTGYFFAYTVIFF
                     VILRFVHGLFWGLSTVSANTIAIDIIPASRRAEGIGFFGVNTNIAMAVAPYFAVNIYD
                     SYGFPVLISTAILMGSLAVGTALLLKTPTKKRSLKVPPISFDRFVLVNAFPILINQLF
                     L"
BASE COUNT         1054 a          712 c          629 g         1322 t
ORIGIN      
        1 ggtaccattt tgcaaaaagt aaccaatatc tttccatgat cctcctttta ttactttgcg
       61 tttcagacct tctggatctt catccttggc atcataacgg atgtcaggat tcaagtcatg
      121 aataaaagag taagcatttt ctgcataagc tgtggatgtc cattcagaca cattaccagc
      181 catacaataa aacccataat catttgcaaa atatgcattg gatttaactg tccaaagacc
      241 tccgtcttcc ggatagtttc ctcttcttgg cttgaagtta gccaaaaggc agcctttgga
      301 atttcttaac ccatatgctc cccatgggta tggggcacta ttgtgaccag cacgggctgc
      361 atattcccat tcaaattccg ttggaagtct gaatccttcg gtatgtacat cctgtggtct
      421 gtacatatcc caaatttttg ttctccatgc acagaaagct gtagcggctt tccaattgac
      481 cccaacaaca ggataatcat cataggccgg atgccagaag tagttacggg tcattggttc
      541 gttgtaagaa tatgcaaaat ccctaatcca aaccatagta tcaggataga ttttggtttt
      601 ttccttttta atataagttt ttctagtgga ttttcctcta tctgctgccg ctttttgcca
      661 atccatccat tcataattaa agaccagctt atctacatca aattcttttt tgccattgag
      721 tatgtcatca cctgagtaaa acatatctgc aagggcttca tccgtatagt cgatctcgtt
      781 atcccaaagg atatggctct tcccattttc atcttcttcg acatccccta aaatagtatg
      841 agcaatagaa tctcttaccc aattgacaaa ttggcggtat tcattgttgg taatttccgt
      901 ttcatccata aaaaagccgg caatcgaaat gcttttaggc ctggaaatat aagtactgct
      961 caggtcttga tcatcagacc caattgtgag agtaccactc ctgacgtaaa tcattccgta
     1021 agggttaatc cctttccagg aaggacgctt gcttacacca atcaattgtc catccatttt
     1081 gctgctcctg ccgcaagagg caacgaaaac cgaaaggatt gctaatccaa ataaaaatgc
     1141 aatgttctta ctcatttgat taaaataatt ttcctaatat tttggctttt tcaattaagg
     1201 gcgtaaaatt agtttgtttc ttcatgtttt ccaaaacaaa gtactttaaa cgcgataatt
     1261 ttaacgatat ttattttaat aatattttaa tattttgaaa atcagcttct tgcaattgat
     1321 tacatttttt atatgtatta ttaaactttc cctaatacat acttcaaacg caaaatgcta
     1381 ctgcaaaagt attacaatat tttgatttgc aactaaattc ttggatgata tattattttt
     1441 tctcttttgg ttgttttaaa atttgtcttt attctaaatt gaactaacaa ttcgtgtgta
     1501 ttacctatat atgcccgttc tttactgatg ttgccttcaa agttatatgc aacaagaatt
     1561 cgttctgtga ttcgtcctcc taacatgacc ttgaccgcat ctattgatga attatcatat
     1621 cctcttatgc caattcctcc aaatatgttt ttgttgtatg ctagaatatc taattccgtt
     1681 tggatagtag ccagatcgta ttttaacaat gctgaaggta atattttcca attgtcatct
     1741 atttgataat aatatgctgt atataaaata aggtgtgtat agggcttata ataataaagg
     1801 tcggaatttc tttttccgat acttgttgtc tgaatagcgg atagacctaa ttcaattgcg
     1861 ttaaaccgac ttactattcc aagattcagg gtgccattgg tactacttga ttttccggca
     1921 ggaatattgg gatcattatg atttatgcca ttttcataat ttccttcagg agtacgtaac
     1981 aagtcaccat tgatcgaact tgtatttaaa ccaagtccga cacctactga gacaaatact
     2041 cttaaaggta ggatgtaatt atagctcatt gtagcagcag atattctggt cgcacctaat
     2101 tcatcatttt tgaagttgag tcctacacca ctatttatcg tacgcaaagg catatgagcc
     2161 gaaatatact gtgttgaagg tcttcccggt aattctgccc attgtcttcg aaagtttcct
     2221 gtaatagtca gagcattatt caatccaccg aatgccggat tataccagta cggctctagc
     2281 atgtacagtc catgttgagc aatctgttgg gcgttgactt tatgagccaa cgctatggtc
     2341 aacatcagca aaacaccggt aaggaaatga aacttgctca ttgtattgta caagaaattg
     2401 tgctctaaga taatctattt gcacaaataa tccgaagtat atttctactg gagttgttaa
     2461 aaaactaaaa gagacaaaag ctccttatac catgtattac tctgaaaacc ttaaaaagca
     2521 agatggaatt tgctctcttg ctttttgaat attcacgtta tatcaggatt cgaccctgaa
     2581 tgaaactttg caaatgacgc catacgatat aatcttgttg tcctgaacat ggcatttaaa
     2641 gtccttcacg tatacggaat caatgttttt aatggttttt gaaatctctt ttacagcatt
     2701 ttgaactgca tcatcgaaac ttttttctga agaggctatg acctcaatta ctttaactac
     2761 tggcataaat ctaatattta taaaattaat aatttcattg tagttcaatt taaagagtgg
     2821 ctttgctgtc agggtgtatt gtgttagggc aacatcaaga aagatggtat tatattaact
     2881 gtgtaatcct aagaaatgtt taaagtgatt aggtggctgc cttattattg attctgccat
     2941 taccttagta tataagtacc ctttaatttt cctgccaatg tgcatactaa gccggtatag
     3001 ttttttcctg atttattaat attggattgt tctaaatttg catttttttt gaaagttata
     3061 tggaaataaa ggagaaactt tggacgcgga attttatcaa tgtcagtgtt tcgaactttt
     3121 tgatagcgtg ttcctttttt ctattgatgc cgactattcc ggtttatttg gctgagcagc
     3181 ttagggtgcc tcattctcaa atcggaattg tgttatcttc ttatgcaata gccttgttga
     3241 ttatccgtcc tttcagtgga tatcttgtgg acatttattc ccgtaaaccg ctatatatta
     3301 taggcattgc tttcttcacc ctgatattca ccggatattt ctttgcttat accgttatat
     3361 tttttgtaat ccttcggttt gtgcatggct tgttttgggg attatccact gtatctgcca
     3421 ataccatagc aattgatatt attcctgcat ccaggcgagc tgaaggaatt ggtttctttg
     3481 gagtaaatac aaatattgcg atggctgttg cgccttactt tgcagtaaat atatatgata
     3541 gttatggttt ccctgttttg atttctactg ctatattaat gggttcactg gcagtcggca
     3601 cagcattgct attgaagaca ccaacaaaga agaggtcatt aaaagttcct cccatttctt
     3661 ttgaccgttt tgttttagtc aatgcatttc ctatcctcat caaccagctt tttcttg
//
LOCUS       DAOQQN010000329         3716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_12108_length_3716_cov_5.546845, whole genome shotgun sequence.
ACCESSION   DAOQQN010000329 DAOQQN010000000
VERSION     DAOQQN010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12108_length_3716_cov_5.546845"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..2042)
                     /locus_tag="PLG24_11055"
     CDS             complement(102..2042)
                     /locus_tag="PLG24_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMZ25475.1"
                     /translation="MGEQNVNKLNNKEEMQLFVRHLLNDVRALETMLRDDWFETDIMR
                     IGAEQEMFMVDTTYYKPALIAVEVLKEMKEYEWVGTELAKFNLETNLTPREFTGSCFS
                     DMEKENNDQLAIIRKFLENYDAKIILTGILPTLNKFDLKLDNLTPKDRYYALMEAFND
                     MLMGSAYELRLQGIDELLVKHDSPLLEACNTSFQVHMQTTPAKFAKHYNIAQSLSGPI
                     IALAANSPIVFGRRLWHESRIALFQQALDIRTSTEHMRDRSPRVSFGKGWLNNSILEI
                     YKEDISRFRVIMTGDKYDDSVDLVAKGKVPKLQALQIHNSTVYRWNRPCYGISDNGKP
                     HLRIENRVIPSGPTVLDEVANAAFWLGAMTGMDDQYKDITKHISWEDVRDNFGKAAQF
                     GIDTKFTWLKDKKITPAELALTELIPLAEHGLKLKGVNDADITRYMDVIRGRADKLMT
                     GARWQLRAFTSLIKHVTRDEAMSVLTQSIIKNQEQEIPIHKWKAPKIKDLEEYRPGTL
                     KVEEFMETDLFTVQKDDIVELVAEMMDWRKIRYMPVEDKKGNLIGLVTSRLLLRHFSN
                     RKVDKKKDVIIVNDIMIKDPITISPDATVLDALEVMRNKQIGCLPVVDKNELVGIITE
                     TDFLRITGRLIDRMEHVEEDGE"
     gene            2200..2730
                     /locus_tag="PLG24_11060"
     CDS             2200..2730
                     /locus_tag="PLG24_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25476.1"
                     /translation="MKHFLPVIVFTLFSAFLFQSCGKETIPKKFTVRLDRTFEVPAGL
                     NYIDAHYFVLDEIPTFYDQIIGQQNVPAGAKITLVPERASITSLFNDIDFDFIDKVAI
                     RIYTNNNPKDKPEAFYIEYIPNSVDNELQLIPTSFDAKPFLEYGIVNAEIRFFFKRTT
                     PQSMNLRIVMDFRGNY"
     gene            complement(3445..>3716)
                     /locus_tag="PLG24_11065"
     CDS             complement(3445..>3716)
                     /locus_tag="PLG24_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Yae1 family protein"
                     /protein_id="HMZ25477.1"
                     /translation="PIKSETMTIAEQLYRKGVKEGKEQGVQEGLEKGMQQGMQQGLEI
                     GMQQGIEKAQFEIIVKLFENGASIDFISSITGLPESKIKEILNLQ"
BASE COUNT         1048 a          809 c          698 g         1161 t
ORIGIN      
        1 cctaaccgta gtatccatta catctaaact aaaaaagccg atccacaatc gaatcggctt
       61 ttatggcaaa gttatttgta taaaacaatt attggaccac cctattctcc atcttcttcc
      121 acatgttcca tccggtcgat cagccttcct gtgatacgca gaaagtcggt ttctgtaatg
      181 ataccaacca attcattttt gtcaacaaca ggcaggcatc caatctgttt gttgcgcatt
      241 acttccaaag catccaatac agtagcatcc ggcgaaatag taattgggtc cttgatcatg
      301 atgtcattga caatgataac atccttcttt ttatcaacct tacgattaga gaaatggcgt
      361 aatagtaagc ggctcgtcac cagaccaatc aggttgccct ttttatcctc tacaggcata
      421 tatctgatct tcctccaatc catcatttca gccaccaact cgacaatatc atctttttgt
      481 acagtgaaga ggtcagtctc catgaattcc tctaccttga gggtcccggg ccgatattct
      541 tcaagatcct taattttagg ggccttccat ttgtggatcg ggatctcctg ttcctgattt
      601 ttgatgatgg attgagtcag gactgacatc gcttcatctc ttgtcacatg cttgatcagt
      661 gaagtaaagg ctctgagctg ccaccgtgct ccggtcatca gcttgtcggc cctacctctt
      721 ataacgtcca tgtagcgggt tatatccgca tcattgaccc ccttgagctt cagaccatgc
      781 tccgctaaag gaatcaactc tgtcaaagcg agttcggcag gcgtaatttt cttgtctttc
      841 agccacgtaa acttcgtatc gataccgaat tgagctgctt taccaaagtt gtcgcgtaca
      901 tcctcccagg atatgtgttt agtgatgtcc ttatactgat catccatacc tgtcatggct
      961 cccagccaaa aagctgcatt ggcaacttcg tccagaacgg taggtccgga aggaatcacc
     1021 ctgttttcga ttctaaggtg tggcttgcca ttatcactta tgccataaca aggcctgttc
     1081 caacgatata ctgtcgaatt gtgtatctgc aatgcttgta acttaggcac tttacctttt
     1141 gccacaagat caacggaatc atcgtatttg tcaccggtca tgataacgcg aaaacgactg
     1201 atatcttcct tgtaaatttc caaaatggaa ttgttaagcc agccttttcc aaaactcacc
     1261 ctcggacttc ggtcacgcat atgctctgtc gaagttcgga tatctaaggc ctgctggaaa
     1321 agagcaatac gcgattcatg ccacagacgc cttccaaaga caatcggcga gttagcagcc
     1381 agggcgataa tcggtccgga cagagactgg gctatgttgt agtgtttggc aaatttggca
     1441 ggtgttgtct gcatatgcac ttgaaaactc gtattacagg cctccagcaa aggagaatca
     1501 tgcttgacca gcagctcgtc tatcccttga agcctcagtt catatgcaga acccatcagc
     1561 atatcgttga atgcttccat cagggcgtaa tacctgtctt taggggtaag attatccagc
     1621 ttgaggtcaa atttgtttaa ggtgggcagg atgcctgtca atatgatctt ggcatcgtaa
     1681 ttctccagga acttccgaat gatagcaagc tggtcattgt tttccttttc catgtcactg
     1741 aaacaagaac ctgtaaactc acgaggagta aggttggtct caagattaaa tttggcgagc
     1801 tctgtaccta cccattcata ctccttcatt tctttcagaa cttcaaccgc tatgagagcc
     1861 ggcttgtagt aggttgtgtc caccataaac atttcctgct ctgccccgat gcgcattata
     1921 tccgtctcaa accaatcatc cctgagcatc gtctccaatg ctcttacatc attgagcaaa
     1981 tgcctgacaa acaattgcat ttcctctttg ttgttcaatt tatttacatt ctgttcgccc
     2041 atgattttgt tcgttgcact attgtgatga aaaatttttc aaatattatt taaaaaaccg
     2101 aaagttgcaa caattatttt ataaaatcaa aaacttttat tgaaaataaa ttttcgaaac
     2161 taattccaac aaaattcttc ataaactcgt tttgatagca tgaaacattt ccttcctgtt
     2221 attgtattta cgctattttc tgcttttctt ttccaatcat gtgggaagga aaccatcccc
     2281 aagaaattca ccgtcagatt ggaccgtacg tttgaagtac cggcaggctt aaactatatc
     2341 gatgcccatt acttcgtgct ggatgaaata cccacttttt atgatcaaat catcggccaa
     2401 cagaatgttc cggccggggc taaaatcacg ctggtgccgg aaagggcctc tattacctcc
     2461 ttatttaacg atatagactt tgactttatc gacaaagtag ctatcagaat atataccaat
     2521 aacaatccca aagataagcc ggaagctttc tacatcgaat acatcccgaa ttcggtcgat
     2581 aacgaactac aacttattcc gaccagtttt gatgcaaaac catttttgga gtatggcatc
     2641 gtaaatgcag aaatacggtt tttctttaaa aggacaacac cccagtcgat gaatctaaga
     2701 atagtcatgg actttcgggg aaactattga atcattgaat aacctgactc tattcaaaat
     2761 attttggaga aatagcagaa taaaaaagga aaatcatggg ccaaatgact tatatccttt
     2821 gggtttttaa cgtgtttagt agttatcagg aagacttttc gggtcataac aagctgatac
     2881 agggataact cataagcgcc ctgtccggat attcccgatt atgaaaggta aataatcagg
     2941 aatatttgaa tgagttgaat gtcttatatt ttatgctcaa taggggatgg ttgtagcatt
     3001 cccggtttat ttaagcttta ttctcctgac ttctgcactt aaaattttac aaatctcata
     3061 aagacctgat aatctatatc atgaatggag tcgggtaaat gttctgggtg gcgcaaggct
     3121 aataaatcat aatttggtga agtttttcca tacgaggaag aaccgttccg gaagctttgt
     3181 agtggttgaa aaataggatg gaaaattcag agagtcaatt atcgcaggca tatcaatcta
     3241 tagctcaatt ttatagagat ggtttacgaa attgcttgcc gggatgatgc actgaccaag
     3301 tcgggaggaa tctaattttg actttgatga atcagctaaa tttttaatag aggcttctaa
     3361 acgaaaatag ttaaagttta aaagcttttt gccaaaaatg tagagattta atcaatagat
     3421 ggcaactaag tcccatcaaa ccgattattg caaattcaat atttctttaa ttttactttc
     3481 cgggagtccg gtaatgctgg atataaaatc gatagatgca ccattttcaa ataactttac
     3541 aattatttca aattgtgctt tttcaatacc ttgctgcata cctatttcga gaccttgttg
     3601 catgccctgt tgcatacctt tttcaaggcc ttcctgtaca ccttgttctt ttccttcttt
     3661 aactcctttg cgatataatt gttcggcgat ggtcattgtt tctgatttta tgggtt
//
LOCUS       DAOQQN010000330         3685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_12259_length_3685_cov_5.289807, whole genome shotgun sequence.
ACCESSION   DAOQQN010000330 DAOQQN010000000
VERSION     DAOQQN010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12259_length_3685_cov_5.289807"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLG24_11070"
     CDS             <1..208
                     /locus_tag="PLG24_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25478.1"
                     /translation="PDKALAHRVVTNMINWLYTRHGHVTYLQDYLNEYCYRYNRHRMK
                     GEILDDIIARMVRHRPIAKKLKLG"
     gene            complement(295..1200)
                     /locus_tag="PLG24_11075"
     CDS             complement(295..1200)
                     /locus_tag="PLG24_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25479.1"
                     /translation="MATNQEKREVVNSTKGFSSSLFLHSMLILIGISPLATFNAPIWE
                     EEQPGMEVALGFPDEGQGNDEPTPGNNEPGGLPPSSSNTTASQTSSPLPTTTSPTPTS
                     SKDEAMVTNDNAEAVDVAAANEAKRRKAEQEQIEAEQRRKAELLAEANRKAEAEKKAR
                     EQAEYEASKGKYGGLFKGSGTGRGNAGKPGNQGDPKGDPNAKNLEGIHTGGKGRIGGG
                     LGNRGVLSEPKIVDNSSKEGRVIIKVCVNSEGTVTSAEFTLKGSSTQDATLVNIARRN
                     ALKFKFTPSDVSQQCGTITYDFRLE"
     gene            1320..3680
                     /locus_tag="PLG24_11080"
     CDS             1320..3680
                     /locus_tag="PLG24_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HMZ25480.1"
                     /translation="MRRIKNIAVLGSGVMGSGIACHLANAGFEVLMLDMSSDGADRNQ
                     IANTALNRALKSSPAPLYSKKFRNRIHTGNFDDDLSRIKNVDWVIEVVKEDLNIKRLI
                     FNKIDAVRKPGTIITSNTSGIPVSLMVEGMSDDFKKHFCITHFFNPPRYLALLEIIPG
                     TDTDPEIIEFLMVFGNKILGKRTVLGKDTPAFIANRIGVFSMGKAFQLTKELGLTIQS
                     VDKLTGVAIGRPKTGTFRLGDLVGLDTAMAVLNGIRRNCPDDALAGDGEIPEFFARLI
                     ENNWLGDKTGRGFYQKTGEKDAKGKPVILGYNLNTFEYEADPGQKLESLDTSKQIDDP
                     EKRIRVLFDYKDAGGLFVRKSLAYTFIYAALRIPEIADNLYSIDDAMRAGFAWDYGPF
                     QYWDIVGLDKGIEAIRELGLEVPEWVVLMKESGNTTFYRNDAGIISFYDPNSRSYKAL
                     PGAEKSIQLNLLADQKVVYKNDEIRLWDIGDDVLCLEFTSKMNAIGGGILNGIQDAIT
                     LAEEGNWRGIVIGNNSKNFTVGANLMMIAMLAYEQEFDQLNMAVDLFQKTTMRCRYSK
                     IPVVAATQGYVFGGGCEMVMHCDATAVAAESYIGLVEVGVGLLPGGGGTKEFAVRASD
                     SFFEGDVQIPRLIERFKTIAMASVATSAVEAYDLGYLNEKDFIVMYQPEAIYRAKQKV
                     IQLSGDYVPPIERDDILVLGRTGLGALYAAAHSLKLGNYASDHDILIAKKIAYVLCGG
                     DLSSPQNVSEQYLLDIEREAFLSLCGEQKTLERISHMLEKGKPLRN"
BASE COUNT          997 a          722 c          836 g         1130 t
ORIGIN      
        1 accggacaag gccctggccc acagggtggt caccaacatg atcaactggc tatataccag
       61 acacggccat gtgacctatc tgcaggacta tctcaatgaa tattgctacc gctacaacag
      121 gcaccgcatg aaaggtgaaa tcctggatga catcatcgcc agaatggtca ggcataggcc
      181 gattgctaag aaattgaaat taggttgatc ttaatatggc aacaggatat gagctaatca
      241 aaccttttca tagtaaacta ataaggtact tggaaattag tgatctcttt ttgattattc
      301 aagtctgaaa tcatatgtaa ttgttccgca ttgttgcgat acatcagatg gagtaaattt
      361 aaacttcagg gcgtttctcc gcgcaatgtt tacaagagtt gcatcctgag tagaagagcc
      421 ttttaatgta aattctgcac ttgtaaccgt cccttcgcta tttacacaaa ccttgatgat
      481 gactctgcct tcctttgagc tattatcgac tatttttggt tcagaaagca cacctcggtt
      541 gcctaatccc cccccaatcc ttccttttcc gccggtatgg ataccttcca aattcttggc
      601 atttggatca cccttgggat cgccttgatt tcctggcttt cctgcgtttc ctcttcctgt
      661 tccggaacct ttaaataagc ccccgtattt acccttagat gcctcatact ctgcttgctc
      721 cctcgccttt ttttcggcct ctgctttacg gttggcttcg gctaataatt cggcctttct
      781 tcgttgctct gcctcaatct gttcttgttc tgcttttcgt ctttttgctt cattcgctgc
      841 cgccacatcc actgcttctg cattatcatt tgtgaccata gcttcatctt tgctcgatgt
      901 tggcgtaggt gatgttgtag taggcaatgg agagctggtt tgagaggctg tagtattgct
      961 tgaagaaggt gggagtcctc ccggttcatt gtttccgggt gtaggttcat cgttgccttg
     1021 accctcatcg ggaaaaccta gtgcaacctc cattcccggt tgttcttctt cccagatagg
     1081 agcattgaag gtagccaatg gggagatacc tatcaagatc agcatactgt gtaaaaaaag
     1141 acttgaactg aatcccttgg tactgttgac cacttctctt ttttcctgat tggttgccat
     1201 aacaactttt ctatataaac gtttaaattg aaatttttct tattattgag gtttcaaaaa
     1261 caaagataca tattgttttt atttttaatg tataatatta atattttcaa taaaagtata
     1321 tgagaagaat taaaaacatt gcagttttgg gctctggggt catggggtca ggtattgcat
     1381 gtcacttggc aaatgccggt tttgaggtgc ttatgctaga tatgtctagt gatggcgctg
     1441 atagaaatca aattgcaaat actgccttaa atagggcact gaaatcatca cctgcccccc
     1501 tgtattctaa gaaattcagg aatcgtattc acacgggcaa ctttgatgat gacttgtcac
     1561 gaataaagaa tgttgattgg gtcattgaag tagtaaagga agatctgaat atcaaacgct
     1621 tgatctttaa taagattgat gctgtcagaa aaccaggtac aattataaca tccaacacct
     1681 caggtattcc ggtaagtttg atggttgaag gaatgagtga tgattttaaa aaacattttt
     1741 gcattactca cttttttaac cctccaaggt acctcgcctt acttgaaatt attccgggta
     1801 cagatacaga tcccgaaatt attgagttcc tgatggtttt tgggaataaa attcttggca
     1861 agcgcaccgt tttgggtaag gatacaccgg catttatagc caacagaatc ggcgtatttt
     1921 ctatgggaaa agcatttcag ttgaccaaag aacttggatt gactattcag tccgttgata
     1981 agttgacagg agtggcaatt ggaaggccta aaacaggtac tttcagactt ggtgacctcg
     2041 taggtcttga cacagcaatg gctgttctga atggtatacg gcgcaattgt ccggatgatg
     2101 cccttgccgg tgatggagaa attccggaat tctttgcgcg tctcatagag aataattggt
     2161 tgggagataa gacgggtaga ggattttatc agaagacagg cgaaaaggat gccaaaggga
     2221 agcctgttat tctgggctat aacttaaata cctttgagta tgaagcagat cccggccaaa
     2281 aacttgagag cctcgacact tccaaacaaa tcgatgaccc ggagaagcgc attagagtgc
     2341 tttttgacta taaggatgcc ggtggtttat ttgttaggaa atccttggct tatactttca
     2401 tttatgctgc tttgaggatt cctgaaatag cagataacct gtattccata gatgatgcca
     2461 tgcgcgcagg atttgcctgg gattacggcc cattccagta ttgggatatt gtgggactgg
     2521 ataaaggtat cgaagcaatc agggaattgg gccttgaagt tccggagtgg gttgttttga
     2581 tgaaagaatc tggcaatacc actttctaca ggaatgacgc aggaataatt tcattctatg
     2641 acccaaattc taggagctac aaggctttac cgggtgctga aaaatctatt cagctgaatc
     2701 ttctcgctga tcaaaaggta gtatataaaa atgatgaaat ccgtctttgg gatatcggtg
     2761 atgatgtttt gtgtctggaa ttcacttcca agatgaatgc tattggtggc ggaatactca
     2821 atggaatcca ggatgccatt acccttgctg aagaaggtaa ttggcgtgga atagttattg
     2881 gcaacaatag caagaacttt acagttgggg ctaatctgat gatgatagca atgttggctt
     2941 atgaacagga attcgaccag cttaatatgg cagtcgatct tttccaaaaa acgactatgc
     3001 gctgtcggta tagtaaaata ccggttgtag ctgcaacaca aggatacgtt ttcggtggag
     3061 gatgtgaaat ggtgatgcac tgcgatgcaa ctgccgtggc tgctgaatca tacatcggcc
     3121 ttgtggaagt gggagtgggc ttactacccg gaggaggtgg taccaaagag tttgctgtga
     3181 gggcatctga ttcgtttttt gagggtgatg tacagattcc gagactaatt gaaagattca
     3241 agacgattgc tatggcttct gtagccacct ctgctgttga agcttatgat cttggttatc
     3301 tcaatgaaaa ggattttata gtcatgtatc agccggaagc tatttaccgt gccaagcaaa
     3361 aagtaattca actttccggt gattacgttc ctcctattga acgagatgat attctggttt
     3421 tgggacgtac agggcttgga gctctttatg cagcagccca ttcattgaaa ctgggcaatt
     3481 atgcatcaga ccacgatatt cttatcgcca agaaaatcgc ctatgtatta tgcggtggtg
     3541 acttgtcatc gccacagaat gtatctgaac aatatttgct cgatatcgaa cgagaagcct
     3601 ttctaagtct ttgtggtgag caaaagactt tggaaaggat ttcgcatatg ttagagaaag
     3661 gcaagccatt acgcaattaa tttat
//
LOCUS       DAOQQN010000331         3662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_12376_length_3662_cov_4.775991, whole genome shotgun sequence.
ACCESSION   DAOQQN010000331 DAOQQN010000000
VERSION     DAOQQN010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12376_length_3662_cov_4.775991"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLG24_11085"
     CDS             complement(<1..241)
                     /locus_tag="PLG24_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HMZ25481.1"
                     /translation="MSVRVRFAPSPTGALHIGGVRTALYNYLFAKKHHGTFILRIEDT
                     DQNRLVEGAEEYIMDALRWAGLDPDESPDNPGDYGP"
     gene            complement(807..1931)
                     /gene="hppD"
                     /locus_tag="PLG24_11090"
     CDS             complement(807..1931)
                     /gene="hppD"
                     /locus_tag="PLG24_11090"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HMZ25482.1"
                     /translation="MDTQIKESKEIGKDILPINGTDYIEFYVGNAKQAAIYYMAAFGF
                     EQVAYKGPETGSRDVVSYVLQQGKIRFVLTSALHPEHEISRHVALHGDGVKVLALWVD
                     DAVASWEGTTARGAESAFEPTVLEDEYGKVTVSAIKTYGETIHTFVQRSDYKGAFLPG
                     YKAWKSERKVEPLGFLYVDHCVGNVELGQMNQWVKFYEDVMGFRLLITFDDNDISTEY
                     TALMSKVVSNGNGYIKFPINEPAQGKKKSQIEEYLDFYKTSGVQHIAIATNDILKTVD
                     GLRKNGVEFLVVPGTYYEDLFDRLGEIDESIEELKRLNILVDRDEEGYLLQIFTKPVL
                     DRPTVFFEIIQRKGATSFGKGNFKALFEAIEREQAIRGTL"
     gene            complement(1989..3143)
                     /locus_tag="PLG24_11095"
     CDS             complement(1989..3143)
                     /locus_tag="PLG24_11095"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HMZ25483.1"
                     /translation="MHYHSAGQIPHKRHIQFRKPDGGLYWEEVFSTEGFSNKYSILYH
                     INPPTRLAQIGKPVDIAPVKAVTEELKHRSLKGFNIKPEDDYIKSRKVVLFNNNVQLS
                     LAAPTKGTEGYFFKNADHDEVIFIHKGSGEFRTVYGSLYFKYGDYIMVPRGTVYQLHF
                     DSDDNRLLIIESTSPFTTPGHYRNEYGQLLEHSPFCERDIVKPTFNEPIDEHGSFRIY
                     IQKMGKLYPYEYLDHPFDVVGWDGYHYPYLFSIHNFEPITGRIHMPPPIHLTFVTKAA
                     VICSFVPRLYDYHPQSIPAPYFHSNIDSDEVLYYVDGEFMSRKHVEKGQITLHPGGVP
                     HGPHPGAIEKSIGAKETGELAVMFDTFSPLFLTKDAIDIEDPDYYRSWLE"
BASE COUNT         1108 a          791 c          656 g         1107 t
ORIGIN      
        1 aaggcccgta gtcaccgggg ttgtcaggac tttcatccgg atccagcccc gcccatctca
       61 atgcatccat gatgtactct tccgcaccct ccacaagtcg gttttgatca gtatcttcga
      121 tccttaaaat aaaagtacca tgatgttttt ttgcaaaaag gtaattatac agcgctgttc
      181 ggacaccccc gatatggagg gcgccggtcg ggcttggagc gaaccttact cttacggaca
      241 ttatttctga attaattgaa acaaagttgc aaaaatatag aatttcctcc agtgaacggt
      301 aaaaattagt cgtattggca tcttatacaa atcaatcaat ccaatagtat ctaaaataaa
      361 tccaaagatt aaaaaggtca attaactaaa ggaatggacc agatctgccc atatccttta
      421 taaaagatcc aattttgtaa attcctattt taaaactctc ctttcgggat taccattatc
      481 cgggaaaaat tccattcgtg aaaagagact gaccaaagat atgtaatgcc tttttccaac
      541 tgcgtcagtc gcataccatt aatacaaatc aactgtgtca aaccttccta tttcttgcaa
      601 ataaagcagc cggacaactt tccttctaca taccgaatat caaaacaaca agcccatccg
      661 taaaatagcg aatgggcttg aactattatt ctaaaaaagc ttcggcaatc gcaaaaattg
      721 gttgtgcaaa taatccatga tttgaaatca catggatcca tgttctgcca attcaaccca
      781 aagcattgaa atccatgaac cggaaattac aatgtacccc tgattgcctg ctctcgctcg
      841 atggcctcaa agagagcctt gaaatttcct ttgccgaatg aggttgcccc ctttcgctgg
      901 attatttcga aaaagacggt tggtctgtct aaaacaggct ttgtaaatat ctggagcaaa
      961 tagccctctt catccctgtc cacaagaata tttaagcgct tcaactcctc gatggattcg
     1021 tcgatctcgc ccaatctgtc aaacaggtct tcataataag ttcctggcac aaccagaaat
     1081 tctacaccat ttttacgcaa gccatcgact gttttcagta tatcattggt tgcaattgca
     1141 atatgctgta ctccggatgt tttataaaaa tcaaggtatt cttctatctg ggatttcttt
     1201 ttaccttgtg cgggttcatt gatcgggaac ttgatgtaac cattgccatt ggataccacc
     1261 ttgctcatca aggcagtgta ttcggtagaa atgtcattat catcaaaagt aatcagcaat
     1321 ctgaaaccca tgacatcctc atagaatttt acccattgat tcatctgacc caattctaca
     1381 ttaccgacac aatgatctac gtaaaggaag ccaagtggtt ccacttttct ttctgacttc
     1441 catgctttat atcccggcag gaatgccccc ttataatcac ttcgctgaac gaaagtatgg
     1501 atggtctcgc cataggtctt aatcgctgaa accgttactt tgccatactc atcttccagc
     1561 accgttggct caaatgctga ttctgcgcca cgggctgttg tcccctccca tgaagcaact
     1621 gcatcatcga cccaaagggc cagtacttta actccgtcac cgtgcaatgc cacatgtctg
     1681 gatatttcgt gctcaggatg aagagctgaa gtcagtacaa aacggatttt cccttgttgt
     1741 agtacatagg agacaacatc cctgctccca gtctctggcc ctttataggc cacctgctca
     1801 aaaccgaatg cggccatata atatattgcg gcctgcttgg catttccgac atagaactca
     1861 atatagtcgg taccattgat tggcaatata tccttgccga tttccttact ttctttaatc
     1921 tgtgtatcca tgataaaatt ttgtattttt aaaaatattt agtcaaatcc aattcttatt
     1981 ccgcaagatc attccagcca actcctgtaa taatcaggat cctcgatatc gatggcatct
     2041 ttggtcagaa ataaaggcga gaatgtatca aacatgactg ccaactctcc ggtttcctta
     2101 gcaccgatac tcttctcgat ggcgccggga tgcgggccgt gtggtactcc acccggatga
     2161 agagtgatct gacctttttc cacatgcttc ctgctcatga attcaccatc cacatagtac
     2221 aatacctcgt cactgtcgat attactgtgg aaatatggcg ccggaatgga ctgtggatga
     2281 taatcgtaca atctcggtac aaaactgcag atgacggcag ctttggtaac aaaggtcagg
     2341 tgaataggcg ggggcatgtg aattcggccg gttatcggct cgaaattatg aatcgaaaac
     2401 agatatggat aatggtatcc gtcccatcca accacatcaa aaggatgatc caagtattca
     2461 tatggataca atttacccat tttctgtata tagatcctga atgaaccgtg ctcgtcgatc
     2521 ggctcattga aggtcggctt aacaatgtcc cgttcgcaga aaggagaatg ctccaacaat
     2581 tggccatatt cgttgcggta atgtccgggt gttgtaaaag gagaagtaga ctcaatgata
     2641 agcaacctgt tgtcatcact gtcaaagtgc aactgataca ctgtacctct tggtaccatg
     2701 atatagtccc catatttgaa ataaaggctt ccatacactg tgcgaaattc tccacttccc
     2761 ttgtgaatga aaatcacctc atcatggtca gcatttttaa agaaataacc ttcagtccct
     2821 tttgttggcg cagccagaga taattgaaca ttgttattga acagaacgac ctttcttgat
     2881 ttgatataat catcctccgg cttgatattg aaacccttca aactacgatg cttaagttct
     2941 tcagtaaccg ctttgacagg agcaatatct accggtttgc cgatttgcgc caatctcgtt
     3001 ggcggattga tgtgatagag aatggaatac ttattggaaa atccttcagt tgaaaacact
     3061 tcttcccaat aaagtccgcc atccggcttt ctgaattgaa tgtgtcgttt atgggggatt
     3121 tgtcccgcgc tatgatagtg cataataata gaagtttttt agaactgcaa agatagcaat
     3181 aaacagagaa ttcttcctat tttaagagta tatatttttc aatactcaat tatcttgaaa
     3241 ttatatttta aattaatgta aattttacat tattattctt ataactacaa attaaaaact
     3301 atcttaggaa ttatataata tttaacattt ggctatatta attaaattat tataccgttt
     3361 aatcaaaaaa tatgcaaata ctttatttta attgcgtggg ataaaaaaaa tacgatttta
     3421 tactttttga aatttttgtg attaagtggg ctattcttta aacaaaaaaa ccaccgattg
     3481 tacgtcggtg gtcggaatag gatttattaa tttaaaaaaa ttaaaaggca gttttcaacg
     3541 tgtggtcaaa atcctggtag tattcaaaaa ttaataaacc tatctcatta aatcggcgaa
     3601 aagaaaattt cacacaatta ttatctctga tttgaaagtt gtagtaataa ggaagtgtaa
     3661 at
//
LOCUS       DAOQQN010000332         3631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_12527_length_3631_cov_4.729306, whole genome shotgun sequence.
ACCESSION   DAOQQN010000332 DAOQQN010000000
VERSION     DAOQQN010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12527_length_3631_cov_4.729306"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLG24_11100"
     CDS             <1..239
                     /locus_tag="PLG24_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25484.1"
                     /translation="YASPVTWITNTSTPTIAFFGSTDSLVPVNEQKTRLDAKFAQVNR
                     PYESYVWNGDHNAYGLEPQLSDVITKSKAFLKKL"
     gene            complement(748..1512)
                     /locus_tag="PLG24_11105"
     CDS             complement(748..1512)
                     /locus_tag="PLG24_11105"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HMZ25485.1"
                     /translation="MNIFPNTLFVGKVCTYLNSVDSTQTYLKDMYANTAPTEGTVIMT
                     YDQLSGYGQRESRWVGDKGKSVSLSTILYPVFLSPVQQFYLSMAVALAVRRSVAILSE
                     QPTYIKWPNDIMIRGKKVSGILIETVLRMSKVKCAFIGIGINVKSRSFGDIDNKAISL
                     EDVSHHTFDQDEVAKILMENIEQYYLKVKSGKYDTIFDEYNNHLYRRGEEIMLENSQG
                     GDFAAKLIGVNKLGMIRLEVDQLTQEYVYGAVRIKY"
     gene            1569..1952
                     /gene="rsfS"
                     /locus_tag="PLG24_11110"
     CDS             1569..1952
                     /gene="rsfS"
                     /locus_tag="PLG24_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HMZ25486.1"
                     /translation="MEVANIPINEVEDSVLADLIVDAIQDIKGENIVKIDLRSVSDAP
                     AQYFIVCEAESTVKINAITNNIYKRVREELGIKPGHMEGGPSAKWNLVDYFSTIVHIF
                     YPETRAFYDLEGLWSDGIFTTYDSI"
     gene            1982..>3631
                     /gene="ftsH"
                     /locus_tag="PLG24_11115"
     CDS             1982..>3631
                     /gene="ftsH"
                     /locus_tag="PLG24_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HMZ25487.1"
                     /translation="MEENNPNSNPEENNKNKKTVKINSYWIYGAVIMLLILGQLLLFP
                     AGSNSKISFDQLKAYTEAGDIKKVNVVNESIAEIYLKKESLNKYPNAVKTTFSESQPN
                     FTMEIGSLTNFETKFDKINDSLTQEQRVEVTYERKTNVIGTILSWLLMPLIFIGLYFF
                     LMRRVSGGAGGPGAQIFNIGKSKAQLFEKDSKVNLTFADVAGLDEAKEEIMEIVDFLK
                     NPKKYTSLGGKIPKGVLLVGPPGTGKTLLAKAVAGEANVPFFSMSGSDFVEMFVGVGA
                     SRVRDLFKQAREKAPCIVFIDEIDAIGRSRGRNNFQGGNDERENTLNQLLVEMDGFST
                     DKGVIIMAATNRADVLDNALMRPGRFDRQIGIDRPDMNGRVQIFNVHLRGIKLNPEVS
                     SVALAEMTPGFAGADIANVCNEAALIAARRNKSSVDMSDFNYALDRVIGGLEKKNSLI
                     SPREKEIISYHEAGHAVCGWFLEHASPLVKVTIVPRGIGTLGYAQYLPKEEYITRTEQ
                     LLDRICMTFGGRAAEEIVFGKISTGAQNDLDQVTKLAYSMIT"
BASE COUNT         1136 a          770 c          695 g         1030 t
ORIGIN      
        1 aatatgcaag tccggtaacg tggataacca atacaagtac cccaaccatt gcattttttg
       61 gcagtacaga ttccctcgtt cctgttaacg agcagaaaac acggctggac gctaagttcg
      121 cccaggtcaa tcgtccttat gagagctatg tatggaacgg cgaccacaat gcctacggct
      181 tagaacctca gttatcagat gtcattacca agagtaaggc ttttctaaaa aaactgtaat
      241 tagcccaaaa tcatataaat gaagaggggc gtataaactt gagggggaga atttattgtg
      301 cctttatgca actaaatgcc tactacctat cacaaataac tggcatttag atacttgcct
      361 tttgcctctc actaacctta tgccttattg ctgtaattat aaaagttgcc ttttaaaaat
      421 cagcaattcc ataatccggg agtatcaggc atctacctaa ttcattttga tcactcaccc
      481 aaataaaacc tcatgtattc ttctatgtct gcctgaaagt caaaacatca actgactttt
      541 ttccaaaaac gaattaggta gttgttcaat actaatcaat tcagtaccta acggactttt
      601 ttctgagaaa agtgaataaa ggaatatcat ctacatcagt ttcaatactt ttgctgtaag
      661 aacttcctat acataacagc cttgagtatt agccacctac ctacttcatt ttgccccctc
      721 actccccttt ccttccaacc tccggcctca atattttatc ctgacagccc cgtacacata
      781 ctcctgcgtg agctgatcga cttccaacct gatcataccc aatttattca ctccaatcaa
      841 cttggcagca aaatcaccgc cctgactatt ctccaacatt atctcctccc ctcgcctgta
      901 aagatgatta ttatactcat caaaaattgt atcgtatttg ccggacttta ccttaaggta
      961 atattgttcg atattctcca tcaatatttt tgcaacttca tcctggtcaa atgtatgatg
     1021 actaacatcc tctaatgata ttgctttgtt atcaatatcc ccaaatgacc tggatttaac
     1081 atttatccca ataccaatga atgcgcattt caccttgctc attcgtagaa ctgtctctat
     1141 caatatgccc gaaaccttct tccctcttat catgatatca ttgggccact taatataagt
     1201 cggttgttcc gaaagaatag ctactgatct tctgactgcc aaagcaactg ccattgaaag
     1261 ataaaactgt tgaacaggtg acaaaaagac aggatataat atagtactca gcgaaacact
     1321 ctttccttta tctccaaccc acctggattc tctttgacca tacccggaca actggtcgta
     1381 tgtcattatt acagtccctt cagtcggcgc ggtatttgca tacatatcct ttaagtaagt
     1441 ttgcgttgaa tctacagaat tcagatatgt gcaaactttt cctacaaaaa gagtgtttgg
     1501 aaatatattc acattatttt tgttaatttg tgcaattata atatttattc atcaaataaa
     1561 aataaaattt ggaagtagcc aacatcccca ttaatgaagt tgaagatagt gtattggctg
     1621 atttaattgt cgatgccatc caggacatta aaggtgagaa tatcgtcaaa atagacttga
     1681 gatccgtatc cgatgcgccc gcacaatatt ttattgtatg cgaagcagaa tctaccgtta
     1741 aaattaacgc cataactaac aatatttaca aaagagtacg tgaagaatta ggtataaaac
     1801 cgggacatat ggaaggtggc ccctctgcta aatggaatct ggtagattac ttttctacca
     1861 tagtccatat tttctatcct gagacacgcg ccttttatga ccttgaagga ctttggtctg
     1921 atgggatatt cacaacatac gacagcatat aaacaattaa taatcaacca ataagcaata
     1981 aatggaagag aacaatccga attctaaccc ggaagagaat aataaaaaca aaaaaacagt
     2041 caagatcaat tcttactgga tttatggcgc agtaatcatg ctgttgattc tgggtcaact
     2101 tctgcttttt ccggcagggt caaattctaa aattagcttt gatcagttaa aggcatacac
     2161 agaagcaggt gatatcaaaa aagtgaacgt cgtcaatgaa agtattgctg agatttacct
     2221 caaaaaggag agccttaata aatatcctaa tgctgtcaag actacttttt cagaaagtca
     2281 accaaatttt acaatggaaa ttgggtctct taccaatttt gagactaaat ttgataagat
     2341 aaatgacagc ctgacccagg aacaaagagt tgaagtgact tatgaacgga agaccaatgt
     2401 cattgggaca attttaagct ggttgctgat gcctctgatc tttattggcc tttacttctt
     2461 tctgatgaga agggtaagcg gtggcgcggg aggtccggga gctcagattt tcaatattgg
     2521 aaagtcaaaa gctcaattgt ttgaaaaaga ctctaaagtc aatcttactt tcgccgatgt
     2581 tgccggcctt gatgaggcta aggaagagat tatggaaatc gtcgattttc ttaaaaaccc
     2641 caaaaaatac acttcccttg gcggcaaaat acctaaaggc gtcctgctgg taggccctcc
     2701 gggaacaggt aaaaccctcc ttgcaaaagc agtagccggt gaagcaaacg tgcctttctt
     2761 ctctatgtcc ggtagtgatt tcgtcgaaat gtttgtcggc gtaggtgcaa gcagagttcg
     2821 tgacttgttt aaacaagcac gtgaaaaagc tccatgtata gtcttcattg acgaaattga
     2881 tgcaatcggt cgttcaagag ggaggaataa tttccagggc ggtaatgatg aacgagaaaa
     2941 cacactaaat caattgttgg ttgagatgga tggattcagc acagataagg gagttatcat
     3001 catggcagca accaaccgcg ctgatgtatt ggacaatgct ttgatgagac ccggccgttt
     3061 cgatcgccag ataggtattg acagacccga tatgaatgga cgtgtccaga tattcaatgt
     3121 ccacctcaga ggtataaaac ttaatcccga agtcagtagc gttgctttag ccgaaatgac
     3181 accgggattt gccggagccg acattgcaaa tgtgtgcaat gaagctgcct tgattgcggc
     3241 aagaagaaac aagagctcgg ttgatatgtc ggacttcaac tatgcactcg atagggtaat
     3301 cggaggactg gaaaagaaaa atagcctcat ttctccgcga gaaaaagaaa tcatttccta
     3361 tcatgaagcc ggtcatgcag tgtgcggatg gtttctcgaa catgcatcgc cacttgtcaa
     3421 ggttacaatt gttcccaggg gtataggcac cttgggatat gcacaatatc tgccaaaaga
     3481 ggaatatatc accagaacag aacagctgct tgacagaatc tgcatgacct ttggcggtcg
     3541 tgctgccgag gaaatagttt tcgggaaaat atccaccgga gctcaaaatg acctggatca
     3601 ggttaccaaa ctggcctaca gtatgataac t
//
LOCUS       DAOQQN010000333         3623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_12568_length_3623_cov_4.515695, whole genome shotgun sequence.
ACCESSION   DAOQQN010000333 DAOQQN010000000
VERSION     DAOQQN010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12568_length_3623_cov_4.515695"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="PLG24_11120"
     CDS             <1..626
                     /locus_tag="PLG24_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HMZ25488.1"
                     /translation="EKVDLENLQMGYDRIFRAFPPPKYQISVVHGKMKAADKEYEMNR
                     FIQHKTNILVATTVIEVGVDIPNASVMIIENADRFGLSQLHQLRGRVGRGAEQSYCVL
                     MTGFQLTLDAKKRLKTMVDTDNGFTIAEVDMQLRGPGSIEGVRQSGGPDFRLANLSSH
                     QDIFQLANEAAIKILTEDPKLDLPKNIGLRKFMATRLKMNKDMSKIG"
     gene            complement(977..3433)
                     /locus_tag="PLG24_11125"
     CDS             complement(977..3433)
                     /locus_tag="PLG24_11125"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HMZ25489.1"
                     /translation="MLRYIYSLVFLGFFTCLLGQPISSDSLYRGFRSASIFRIAVHTM
                     PPEIDGDLSDDVWKAAREYSGFTLFEPNPGAACSRNTYFRLLYDDNAIYIYARMEDDP
                     ANILKELAPRDQYNNTDYIEFFFDPYASGTVAFSFAVTPRNLQRDTKYIGSQEEDESW
                     DAIWDSAVQSTEDGWQAEFRIPFSQLRFPEEDVQQWRFNVVRQMKKVREKSSWQILNP
                     AISGLINQNGYLSGISHLKSPLRLSLFPYLSAYYAPEAGAKMKFAGGLDLKYGINDAF
                     TLDMTLIPDFGQVAFDNIVYNLTPFEVQYEERRPFFTEGLELFSRAELFYSRRIAADN
                     HYFDRNGIPDGKTLVEVPAKNKLINAFKLTGRASNGLGVGILNALEGRSHAVLKDDKT
                     GQTERIPVNPISNYNIIVVDQNLRNNSYISFINTNVMRQGEHRDGNVTGAELNIRSKN
                     QAYYIKAIGALSYISQGREAKPGYKYAFSTGKNSGNWTTSIEYEEISRRFDPTDLGYL
                     KVFNTRAAEWNLGYSTYIPRGIRNKSTTSLSVNYERVISPDRFSNFGIEGEYFFLDRN
                     FNALNLDLGINPIITYDFYEPRSGDFSRFYTFPIGYEIGGFISTDYRRPLALDMNFGH
                     RHVNEAGRNYYQINADPRMRFNDHFSIFATIGFERGYNDVGYAYTIRHEDEPWRVNDI
                     IFSRRNVQTVVLGFNPVLLINPNLNFSLRVRNYWSQVSIKSLHLLETDGYLSEDEVAG
                     GNYPLFQLVQFTNFEARANWRFAPGSDLILAWNSGYDKFTNEDSRYWSSYSNFGNRIR
                     NNIISVRVNYYLDYARLFRK"
BASE COUNT         1055 a          829 c          773 g          966 t
ORIGIN      
        1 gtgaaaaggt tgatctcgaa aacctgcaaa tgggttacga cagaatattc cgggcatttc
       61 caccccctaa ataccagatt tccgtcgtgc atggcaaaat gaaggctgcc gataaggaat
      121 acgagatgaa ccgatttatc cagcataaaa ccaatattct ggtagctact acagtgatcg
      181 aggttggtgt ggacatcccg aatgcatctg tcatgatcat cgaaaatgca gaccgttttg
      241 gcctctccca gttgcaccag cttcggggac gtgtcggtcg cggtgcagag caatcctatt
      301 gcgtactgat gacaggattc cagctcacac ttgatgcgaa aaagagatta aagaccatgg
      361 tcgataccga caacggattt accattgccg aagtggatat gcagcttcga ggaccgggaa
      421 gcatcgaagg tgtacgccag agcggcggtc ccgactttcg tctggctaat ctttcctccc
      481 atcaggatat cttccagctg gccaatgaag cagccatcaa aattctgaca gaagacccga
      541 aactggattt gccaaaaaac atcggattga ggaagtttat ggctacccga ttgaagatga
      601 acaaggacat gtcgaaaatt ggctgatcct tgaatgcacg cctgcttcac agccctatcc
      661 tgaccacact tttgattttc ttcacaagca acctgtactg aaacccgcta ttcggtcaag
      721 cattttctac tccccggatc tgtcagacga ctcaacacta ttaccgatct tataccagga
      781 tgatttatat aatagatttc aaacttgagg tataataaca aatgtacctt cggtattaga
      841 caccggaaat gctttttgac gccttggatg accatatcaa tggtcgatgc atattcactt
      901 caaatagcca atgtagctta ggaatatgcg cttgatctgt ctatgagttt atttaattga
      961 cttccgttca ttagaatcat ttcctgaaca atctggcata atccagatag taattgacac
     1021 gtaccgaaat aatattgttc cggattctgt taccaaaatt gctataggaa gaccaataac
     1081 ggctgtcctc attggtaaat ttgtcataac cactgttcca cgccagtatc agatcactgc
     1141 ccggtgcaaa ccgccagtta gcccgggcct caaaattggt aaattggaca agttgaaaca
     1201 atggataatt gcctcctgca acctcgtctt cagaaaggta accatctgtc tcaagcaggt
     1261 gcaaagattt gatactcacc tgtgaccaat aattcctcac gcgaaggctg aaattgaggt
     1321 ttggattgat aagcaggacg ggattgaaac ccagaacaac agtctggaca ttccggcgtg
     1381 aaaagataat gtcattgacc cgccatggct catcttcatg tctgatggta taggcatacc
     1441 cgacatcgtt gtatcccctt tcgaaaccga tggtagcaaa gattgaaaaa tggtcattga
     1501 aacgcatcct gggatcagcg ttaatctggt aataatttct gcccgcctca ttcacatgac
     1561 gatggccgaa attcatgtcc agagccaggg gacgccggta atccgtcgat ataaagcccc
     1621 cgatttcata accgattgga aaggtataga atctactgaa atcgccgctg cgaggttcat
     1681 aaaaatcata ggtgattatc ggattgatgc ctaaatccag attaagtgca ttaaaatttc
     1741 tgtccagaaa aaaatattcc ccctctatac caaaattgga aaatcggtca gggctgataa
     1801 ctctctcata attcacactg agtgatgtag tggacttatt tctaatacct ctgggtatat
     1861 aggtggagta gccaagattc cactctgccg ccctggtgtt aaatactttg aggtaaccaa
     1921 gatcagtggg atcaaacctt ctggagattt cttcatattc aatggaagta gtccagttac
     1981 cggagttttt accggtgctg aatgcgtatt tataccccgg ctttgcctcc cggccctgac
     2041 tgatatagga gagcgcgccg attgccttga tgtagtatgc ctggtttttg cttcggatat
     2101 tcaactctgc cccggtaacg tttccgtcac gatgctctcc ttgtcgcatg acattggtat
     2161 tgatgaagga gatatacgaa ttattcctga ggttttgatc gacgacaata atattgtagt
     2221 tggagattgg attgaccgga atcctttcag tctgcccggt tttgtcatcc tttaatacag
     2281 catggctcct gccctccagc gcattcagaa tccccacacc aagtccatta gatgccctgc
     2341 ccgtcagctt gaatgcattg attaatttgt tttttgcagg aacctccact aacgtcttac
     2401 catcgggaat tccattcctg tcaaagtaat gattatccgc agcaatccgt ctggaataaa
     2461 acagctctgc tctcgaaaac aattccaggc cttccgtaaa aaaaggcctc ctttcttcat
     2521 actgcacctc aaaaggcgtt aggttgtaaa caatattgtc gaatgccacc tgaccaaaat
     2581 cagggatcaa ggtcatatca agggtaaatg catcattgat accgtacttt aggtcaagtc
     2641 ctccggcaaa tttcattttt gctccggctt ccggtgcata atatgctgac aaataaggaa
     2701 atagtgaaag ccgcagtggt gacttcaaat ggcttatccc ggaaaggtag ccattttgat
     2761 tgatcagacc actgatggcg gggttgagaa tctgccaact ggatttttca cgaacttttt
     2821 tcatttggcg gacgacattg aagcgccact gctggacatc ctcttcaggg aaccgcagtt
     2881 gggaaaaagg aatccgaaac tctgcttgcc agccatcttc agtagactgc acagcactgt
     2941 cccagatagc atcccacgac tcatcctctt cctgtgaacc aatgtacttg gtatcccgct
     3001 ggaggttgcg aggggtcact gcgaagctga aagccacagt tccggacgca taaggatcaa
     3061 agaaaaactc gatatagtcg gtattgttgt attgatcgcg cggtgccagt tctttcagga
     3121 tattggcagg atcatcctcc atcctggcat aaatatagat cgcattgtca tcataaagca
     3181 gccggaaata ggtatttcgc gaacatgctg caccgggatt gggctcaaaa agagtaaagc
     3241 cggaatattc ccttgccgcc ttccatacat catcagaaag gtctccatcg atctcaggag
     3301 gcattgtatg cacagctatt ctgaaaatgg aggcagacct gaatccccgg taaagagaat
     3361 ccgaagagat tggttgcccc aacaagcacg tgaagaatcc aaggaagaca agtgaataaa
     3421 tatacctcaa cataatacgt ttgctgacta ttgcaaatat ggtgaaaagt attaaggtga
     3481 tgataattcg gtacttgaaa aatcaacatt ttagaccaac tttattttca caccactgat
     3541 ctaccaataa ccgggaataa aaaaattaga ataatttcac aaaaattaaa aaatcataat
     3601 ttatgagaaa agataatatg cag
//
LOCUS       DAOQQN010000334         3621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_12575_length_3621_cov_6.028603, whole genome shotgun sequence.
ACCESSION   DAOQQN010000334 DAOQQN010000000
VERSION     DAOQQN010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12575_length_3621_cov_6.028603"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /locus_tag="PLG24_11130"
     CDS             complement(<1..1551)
                     /locus_tag="PLG24_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ25490.1"
                     /translation="MSFLLKYLRKHTWLIILTLLLAAIGQIFSMMDPYIMRLLIDGYT
                     KDLDKFSSDDFIRGVLTLGLAIIGVAMVSRIAKAFQDYMVNLITQKVSTEIYNDGLQH
                     ALSLPYEDFEDQRSGETLGILQKVKTDVQRLLSTTINILFVSLVGIVAVTIYSFSVYW
                     AVGPLFIITIPVLIVLSGYLTKKIKKIQKRIVSETTALAGSTTESLRNIELVKSTGLV
                     DQEINRLNDTTNRILDLEIQKVKYVRSLSFIQGTVINFLRTSIIVIMAWLIFLGKISV
                     GEFFSLFMYSFFIFGPLQEIGNLINIYSETLVSLQNFKQLMARKPEMAPEQPVHIDSI
                     DNITFNNVCFTHQSANKPALSNISFDVRKGEKIAFVGPSGAGKSTLIKLLFGLYKPKK
                     GRVAYSDIPIESIDINDIRHQIGIVNQETHLFAGTIRDNLLFVKPDASDEELEKVLRM
                     AAADSLLDRADKGIYTTIGEGGIKISGGEKQRISIARALLRNPNLLVFDEATSALDSL
                     TEEEINETI"
     gene            1822..>3621
                     /locus_tag="PLG24_11135"
     CDS             1822..>3621
                     /locus_tag="PLG24_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25491.1"
                     /translation="MAKQIPPRKRNTRSNNPLTNPSSDQEVQHSVDPENRDERIIITG
                     IVGDITPEYSLDGFTPVMSVELDASRGVASEDIEVDSNLLAQVVFSDGTVWCGYVGDL
                     PEIYTQMGISRGAGGSMKLPELLTSTSDGRGDGGVFRRLVNVFRPEAMSYTAHTIALK
                     VDRNIMAAPGLYTVDASFQLLPLKDGAILSGTVLLLLHGTLSSTKGSFGDMTTGGMWE
                     AIYSKYDHIIALEHHTISETPLKNARDVLQALPPGLSVDILSQSRGGLIGDVLTRCDY
                     RNDHPGYSSDERAILVREDKTLGVLVDELNGLAKEKRLTVRRFIRVASPSGGTSVLGQ
                     RMDIFLNAFLNITGLVFGVGVTPVYQAVREFIMEVLSHRFKASDMPGMWSMVPSSAYQ
                     RVNNMQHDYHTEVFVIAGDGKAGGKITNSLLVIFTNLYYRAPNDFVVDTASMNKGVSG
                     PSGIQYFLSNTNKTNHFGYFRNEDSRWALQYAFRMVNNGEESPFSDVGGDKDSSRGIG
                     LDLVMKRYSTNKVSGTRPIVIILPGIMGSTLCDNVDDIWLSIPRISAGAFVNKLNINA
                     TNIGTSGVMEKYYRKIGEFMSLNYDVEVFPFDWR"
BASE COUNT          985 a          718 c          840 g         1078 t
ORIGIN      
        1 aatggtttcg ttaatttcct cttcggttag actatctaag gcagaggtgg cctcgtcaaa
       61 aaccagcaga ttgggattgc ggagtagcgc tctggctata gaaattcgct gtttttcacc
      121 acctgagatt tttataccgc cttcaccgat ggtagtgtag atgcccttat ctgctctgtc
      181 cagcaaacta tctgcagccg ccattctcaa gaccttttcc aattcttcgt ctgaggcatc
      241 cggcttgacg aaaagcaaat tgtcgcggat agtacctgcg aagagatgtg tctcctgatt
      301 gacaatgcct atctggtgtc ggatatcgtt gatatcgatc gactctatcg gtatatcgga
      361 ataggcgacc cgaccttttt tgggtttata caatccaaat aacagcttta ttaaagtact
      421 ctttccggca cctgatggcc cgacaaaggc gatcttttcc cctttccgga catcaaagga
      481 tatattggaa agtgcaggtt tgttggcact ttgatgcgta aaacaaacgt tgttgaatgt
      541 tatgttatca atcgagtcga tatggacagg ttgttcaggt gccatttccg gtttccgggc
      601 catgagttgc ttaaaattct gcagacttac tagtgtctcc gagtaaatat ttatcagatt
      661 tcctatttcc tgtagcggac cgaaaatgaa gaaactgtac ataaacaaag aaaagaattc
      721 acctacgctt atctttccaa ggaatatcaa ccatgccata atgactatga tactggttcg
      781 tagaaaattt ataactgtac cttgaataaa ggaaaggctt cgcacatatt tgaccttctg
      841 tatttcaagg tcaaggattc ggttggtggt atcattgagc cgattgattt cctgatctac
      901 caggccggta ctttttacaa gctctatatt gcgcagggat tcggtagtgg agccggctaa
      961 agctgtagtc tctgagacaa tacgtttttg tattttttta atttttttcg tcagatatcc
     1021 acttagcaca atcaggaccg ggatagttat gatgaagagt gggcccaccg cccaataaac
     1081 cgaaaacgaa tagatggtga cagctacaat gcctactaat gagacgaaaa ggatattgat
     1141 tgtggtggaa agcagtcttt gtacatctgt cttgactttt tggaggatac caagtgtctc
     1201 gccgctacgc tgatcttcaa agtcttcata gggtagacta agggcgtgtt gcaagccatc
     1261 gttataaatc tcagtactta ctttttgtgt aatgagattg accatataat cctgaaaagc
     1321 ttttgcaatc ctggaaacca tggcaacgcc gataatagcc agcccaaggg tcaaaacacc
     1381 acggataaag tcatcactgg aaaatttatc cagatcctta gtatacccat cgattaacaa
     1441 tcgcatgatg tacggatcca tcatactgaa tatctggccg atcgctgcca gaagcaatgt
     1501 cagtatgatc agccaggtat gttttctaag atattttaac aggaatgaca tcagaaaatt
     1561 tacattcaaa caagctaatc atccgttggt ttaatcatca caatgaatgt agcctaattt
     1621 atattcattt aattgaaaat ttgcattagt ttggttatat ttggcaaata ttaccatgaa
     1681 aatcaatcct catatctttt aaacgagggt taaattcttt ctttaagtag ggtcttgaaa
     1741 tatttctgta aatagctgta tttgtaatgt cagatgcagt gcttcacctg tgatggtgat
     1801 taatcccaaa tatgagtaat tatggcaaag cagattcctc cacgaaagag aaacacaagg
     1861 agtaataatc cacttacgaa tccatcttcc gaccaggaag tacagcattc tgttgatccc
     1921 gaaaatcggg atgaacgtat tattataacc ggtatagtcg gagatataac acctgagtat
     1981 tcattggatg gtttcactcc ggtgatgtct gttgaattgg atgcttcccg gggagttgct
     2041 tcggaggata ttgaggtgga tagtaatctg ttggcacagg tagtgttcag tgatggtaca
     2101 gtctggtgtg gctatgtcgg cgatttgccc gagatttata ctcaaatggg catctccaga
     2161 ggtgcaggtg gatcaatgaa gctacccgaa ttgcttactt ctacttcgga tggaagaggg
     2221 gatggtggcg tttttcgtag gttggtcaat gtattcaggc ccgaggcaat gtcttataca
     2281 gcgcatacaa tcgcccttaa agttgaccgg aatataatgg cagcacccgg gctttatact
     2341 gtagatgcgt cttttcaact ccttcctctc aaggatggtg caattttgtc tggtacggta
     2401 cttttattgt tgcacggcac cttgagttca acaaaaggct cttttgggga tatgactacc
     2461 ggtggcatgt gggaggcaat ctacagtaag tatgatcaca tcattgctct ggaacatcat
     2521 actatttccg aaacacctct gaaaaatgcg agggatgtgc tgcaggctct gccaccggga
     2581 ttatcggtag atatactttc tcagtcgcgc ggtggcctca taggtgatgt cctgacccga
     2641 tgtgattatc gcaacgacca tccgggctat tcgagtgatg aaagggcaat attggttcgg
     2701 gaggataaaa cattaggtgt attagtagat gaattgaatg gactggcgaa agagaaaaga
     2761 ttgactgtgc gtcgtttcat cagggttgcc agcccaagtg gtggaacatc agtgttgggc
     2821 cagaggatgg atatcttcct caatgccttt ctgaatatca cagggcttgt attcggcgta
     2881 ggtgtaacac cggtttatca ggcagtgaga gagtttatta tggaggtcct ttctcaccgg
     2941 tttaaggcat cggatatgcc tgggatgtgg tctatggtgc cttcctcagc ctaccaacgt
     3001 gtcaacaaca tgcagcacga ctaccataca gaagtatttg tgattgccgg tgacggtaag
     3061 gctggcggta agataaccaa ttcactgctg gttatattca ctaaccttta ttaccgggcg
     3121 ccaaatgatt ttgtagtcga tactgcttca atgaataaag gtgtaagcgg tccatccggc
     3181 atccagtatt ttctttcgaa taccaacaag actaaccatt ttggctattt cagaaatgaa
     3241 gattcccgtt gggcgctgca atatgccttc agaatggtga ataacggtga ggaatcgcca
     3301 ttttccgatg tagggggcga taaagattcc agccgcggca ttggtctgga tcttgtgatg
     3361 aaacgttatt caaccaacaa ggtgtcgggg acacggccaa ttgttatcat tctgccgggc
     3421 atcatggggt caaccctctg tgacaatgta gatgatatct ggctatccat tcccaggatt
     3481 tctgcaggtg catttgtcaa taagttgaat atcaatgcca ctaatattgg aactagtgga
     3541 gtcatggaga agtattacag aaagatcggg gaattcatgt ctttaaatta tgatgttgaa
     3601 gtttttcctt ttgattggcg t
//
LOCUS       DAOQQN010000335         3573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_12842_length_3573_cov_4.381182, whole genome shotgun sequence.
ACCESSION   DAOQQN010000335 DAOQQN010000000
VERSION     DAOQQN010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12842_length_3573_cov_4.381182"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PLG24_11140"
     CDS             complement(<1..723)
                     /locus_tag="PLG24_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ25492.1"
                     /translation="MSLKNLYILSCLLLLPLWAHATHNRAGEIIFQQIGELRIRASII
                     TYTKESSHDADRDTLILYWGDGTFQKVGRNNGSGHGISLGGDVKYNIYTYEHTYPSRG
                     TYTISMTDPNRNGGILNVNPPNSDNVPFYLETTFTFLNSTFQGYNNTPTLLQPPIDEG
                     CIDQPFIHNPNAFDIDGDSLAYELIVPMMAKNTPVPNYIFPDQIVAGANNILTLNPIT
                     GDIVWNAPKKAGEYNIAIRVNEY"
     gene            complement(1278..1835)
                     /locus_tag="PLG24_11145"
     CDS             complement(1278..1835)
                     /locus_tag="PLG24_11145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HMZ25493.1"
                     /translation="MIIDNTWTLFLDRDGVINRRILGGYVTKIEEFEPLPGIFEAIRT
                     FNKIFARTCIVTNQSGVGKGLMSQEDLNNIHSHFMDLLNEKNAIIDGIYCCTSLETDI
                     DNCRKPLPDMAIRAKQDFREIDFSRSIMVGDTGSDMEFGYNIGALNVLILDGSEHQKK
                     IDEELVNLRFPSLLEFSQFLVASYN"
     gene            complement(1848..2435)
                     /locus_tag="PLG24_11150"
     CDS             complement(1848..2435)
                     /locus_tag="PLG24_11150"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HMZ25494.1"
                     /translation="MNYSRDISKILSEHENVVKSVINDSTLLAAIEEFTRLVVQCYTH
                     GGKILLCGNGGSASDAQHIAAEWQGRYLLERKPLHAEALHANTSYLTAVANDYGFDKV
                     FSRAVEAQGKGGDILVGLTTSGNSPNIVNALKTGKSLAMTCIGMTGNKTDPAIEPYCD
                     LMIKVPSDVTARIQEIHILIGHIVCELSEKSLFKS"
     gene            complement(2432..3385)
                     /locus_tag="PLG24_11155"
     CDS             complement(2432..3385)
                     /locus_tag="PLG24_11155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ25495.1"
                     /translation="MTNERIQISVVVPLYNEDESLPELAAWIHRVMKANGFTYEIIMV
                     DDGSKDQSWEVIERLSKENNSVKGIKFKRNYGKSAALHEGFCASTGDVVITMDADLQD
                     SPDEIPELYKMIKEKGYDLVSGWKKKRYDPLSKTIPTKLFNAAARAMSGIRLHDFNCG
                     LKAYKGKVVHSIEVYGEMHRYIPVIAKGAGFGKIGEKVVQHRERKYGVTKFGLDRFIN
                     GFLDLLTIFFVGRFGKKPMHFFGTLGIIAFFIGFIILIELSIEKIFFKVGGIADRPLF
                     FLGMLATIIGSQLFLTGFLAELITRNSPSRNNYLIEEKIHL"
BASE COUNT         1016 a          719 c          716 g         1122 t
ORIGIN      
        1 gtattcgttt acccttatgg ctatgttgta ttcgccggct tttttgggcg cgttccagac
       61 gatgtcgcct gtgataggat tgagagtcag aatattatta gctccggcaa caatctgatc
      121 cggaaagata taattgggaa ccggtgtatt tttagccatc atcggtacga tgagttcata
      181 tgccaggctg tcaccatcta tatcgaaagc atttggattg tgaatgaaag gttggtcgat
      241 gcagccttcg tcaataggtg gctgtaatag ggtaggcgtg ttgttatacc cctggaaggt
      301 tgaattaagg aaggtaaatg ttgtttccag atagaaaggg acattatctg aattgggagg
      361 gttgacattg aggatgcctc cattgcggtt tgggtcagtc atactgatcg tgtacgtacc
      421 tcgagaagga tatgtatgtt cgtaagtata aatattgtac ttcacatctc ctccaagaga
      481 aatcccatgt cctgagccgt tattgcggcc tactttttgg aaggttccgt cgccccaata
      541 aagaatcaat gtatctctat ctgcatcatg gctggattcc ttggtatatg tgatgattga
      601 cgctctgatg cgcaattctc cgatttgttg gaaaataatt tcacctgccc tgttgtgggt
      661 tgcatgtgcc catagcggaa gtagcaacag acaggacaag atatataggt tcttcaatga
      721 caaattgacg tacttgtttt ttcaaaaata cgaaattcga agcaaatgtt tttgaaaaac
      781 tcagatttaa ctattccggt gatgataaag ggtgggaaaa aggtctttgg ggaagagtcg
      841 ttttgaagta ggtagatgat taatactact aaattcaata gctatgaaat gttgacacaa
      901 tgactaatta tttttggctt ggttttagtg aaatgattaa tattaatttt tcaatttgct
      961 ttataacata gggatgcgag gtattagaca tctacctaat tctaattttt cactttcaaa
     1021 gtgattttac attccttcca aatcgaagta ggtagatgat taatactgtg caatccaggc
     1081 attataaatc gcttgcagta atttgaaaaa aatcacaagg ttttctacct tattattgag
     1141 ctaataatcc taaaatgcct ttggaaaaag agtttgggtg tattaagcaa atacctaatt
     1201 ctacataatg ttataataca cagataaaca atagtatata catataataa atgcaatatt
     1261 tatttatctc ccacccccta attgtaggaa gccaccaaaa actgtgaaaa ctcaagtaag
     1321 gagggaaatc gaaggttaac tagttcttca tctattttct tctgatgctc ggaaccatcg
     1381 agtatcaaaa cattgagggc cccgatatta tatccaaact ccatgtcaga ccctgtatca
     1441 cctaccatga tggacctgga aaagtctatt tcccggaaat cctgttttgc cctgattgcc
     1501 atatctggaa gcggcttgcg gcagttgtcg atgtctgttt ctaaggatgt acaacaatag
     1561 ataccatcga tgatggcatt cttttcattg agtaagtcca taaaatgaga atggatgtta
     1621 ttaagatctt cctggctcat caatccttta cctacccctg attgattggt gacaatacat
     1681 gtacgagcga atatcttatt gaatgttcta atggcttcaa atattcccgg caaaggctcg
     1741 aattcttcaa tttttgttac atagcctcca aggatacggc ggttgatgac accgtcccgg
     1801 tcaagaaaca aggtccatgt attgtcgatg atcaaaatag ttggaattta agacttgaag
     1861 agactttttt cagacaattc acacacaata tgaccgatga ggatatgtat ttcctgaata
     1921 cgtgcagtca catctgaagg cactttaatc atcaggtcgc aatatggctc aatcgcggga
     1981 tctgttttat ttcctgtcat acctatacag gtcatagcca gtgatttccc ggttttcaaa
     2041 gcattgacaa tattgggaga gttccctgag gtagtcagac ccaccaaaat gtctccgcct
     2101 ttgccttgag cttctacggc ccttgaaaac actttgtcaa atccataatc attagccact
     2161 gcggttagat aggatgtatt ggcgtgtagt gcttccgcat gtagtggctt cctttccaac
     2221 aggtaccttc cttgccattc tgcagcgata tgttgagcgt cagaagcgct tcctccattg
     2281 ccacacaata atatcttgcc tccatgagta tagcattgta ctactaacct cgtaaattct
     2341 tctatggcag caagcaaggt gctgtcattg attactgact ttacgacatt ttcgtgttct
     2401 gaaaggattt tagatatgtc ccgggaatag ttcaaagatg gattttttct tcaattagat
     2461 aattattacg cgaaggactg tttcttgtta ttaattctgc taaaaagccg gttaggaaaa
     2521 gctgagatcc gatgatggtg gccagcatac ccaggaaaaa cagtggtctg tctgcaatcc
     2581 cacctacctt gaagaatatc ttttcaatac tcaattctat gaggataata aagccgataa
     2641 agaatgctat gatgcctaaa gtgccaaaaa agtgcattgg tttcttgcca aagcgaccca
     2701 caaagaatat cgttaaaaga tccagaaatc cattgataaa tcgatctaaa ccgaatttag
     2761 tgacgccata ttttctttct cgatgctgga cgaccttttc gcctatcttt ccaaatccgg
     2821 ctccttttgc aattaccgga atataacggt gcatctcacc atatacctct atagaatgta
     2881 ccaccttgcc cttataagct ttaagtccgc agttgaaatc atgcagtcga attccgctca
     2941 tggcacgagc tgctgcattg aataacttgg tggggattgt cttgcttaat ggatcgtaac
     3001 gttttttctt ccagccggaa accaggtcat accctttttc cttaatcatt ttgtacaatt
     3061 caggtatttc gtccggacta tcttgcaaat ctgcatccat ggtgattacc acatctccgg
     3121 tggaggcaca aaagccttca tgtaaagcgg cggatttgcc ataatttctt ttgaatttta
     3181 tccctttgac gctgttgttt tctttactga gtctttcgat tacttcccat gattgatcct
     3241 tgcttccatc atccaccata atgatttcgt aggtgaaccc attggctttc atgacacggt
     3301 gaatccaggc agcgagctcc ggtaaggatt catcctcatt gtataaagga acaacaaccg
     3361 atatttgtat tctttcgtta gtcatgcaac cattttaatg cttcgtcgga caatagtata
     3421 tgaataatca ataaaccggc ttgttttttt gaaatattgc acccaggatg gccccaataa
     3481 tagctccgaa aaccaattga cccagaaagg ctgcagttga aagtacagcc ggtgacctaa
     3541 agaccgctgt aatgctattg gccatttcaa tct
//
LOCUS       DAOQQN010000336         3573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_12843_length_3573_cov_4.253553, whole genome shotgun sequence.
ACCESSION   DAOQQN010000336 DAOQQN010000000
VERSION     DAOQQN010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12843_length_3573_cov_4.253553"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLG24_11160"
     CDS             <1..300
                     /locus_tag="PLG24_11160"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HMZ25496.1"
                     /translation="PSVSPKKTVEGTLGGIIATIALAWPLSMINDAFTYPQWLIVATI
                     TGVSGTIGDLVESKLKRHYGIKDTGTYLPGHGGFLDRFDSFLFVVSFVYLYTQLI"
     gene            307..960
                     /locus_tag="PLG24_11165"
     CDS             307..960
                     /locus_tag="PLG24_11165"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HMZ25497.1"
                     /translation="MFPIHREGRVTILIAILFSILIWWIFNSFLQELMWLGYALIIFV
                     LVMVLQFFRNPRRQVPAIDEDIVIAPADGKVVVIEKVQEEEVLKCKCIQVSIFMSPLN
                     VHVNRNPLSGIITHNIYHPGKYLTAWNPKSSTENERTTVVYQHDKGTVLMRQVAGALA
                     RRIVNYLKEGQSVRRGDEMGFIKFGSRVDLYFPPETEILVSVNQIVKGNITRIARLK"
     gene            complement(1009..>3573)
                     /locus_tag="PLG24_11170"
     CDS             complement(1009..>3573)
                     /locus_tag="PLG24_11170"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMZ25498.1"
                     /translation="LQAELEKVTQLKNVKVNGAAERIVRVDLHLEKMAHLHIPALLVA
                     NALQSESANIPGGNIIEGTKSFNVKTNSNYSNLQEIRNTIITGRGVNTILLKDIADVY
                     YDYGPDTYITRLNGHRCIFVNAALKPGENISLAQESYKPILEKFRAGLPSNIDMQAHF
                     DQADNVNDRLYNLGLDFLIAIGLVLITLLPLGNRASLVVMIAIPLSIGIGVILLNLFG
                     YNLNQLSIVGLVVALGLLVDDSIVVVENIERWIREGHSRLDATIKGTGQIGMAVLGCT
                     ATLIIAFLPLAFMPEGSGDFIHSLPLAVIFTILGSLIVALTIVPFLSSRILKPAEGDH
                     SNIFLRGLQKVIHGTYARLLDVALRHPVLSLLIALSIFVGSLFLIPVVGTSLFPASEK
                     PQFTIDITAPLQSGIYNTDSITRQIEAELAKMPEVKYYSSNIGKGNPRIYYNVIQRNE
                     QADFAQIFVQLHPDIRPDEKLNIIKELRRRWTPYPGAEVKVVNFEQGIPQIAPIEVRL
                     LGDNLDTLRQLASEVENMLVANPGAIYVSNPVKNNKTDIRIHIDKEKAQSLGVPTINI
                     SQTVRMALAGIPIGTYTDKEKNNNDYTIVVTKPRESYPDLSVFRNLFVNASDGRAIPI
                     DQITDIGFETSAQTINHLNKVRTVSVTAFIREGHLNSAVINDVVKALEQKKFPKGYSF
                     TMGGEVEGREESFKGFGYIILVTVFLFIAVLVLQFRTFKSTLIVLSVIPLGIVGAVLA
                     LLVTGNSLSFVATVGIVALAGIEVKNTILLVDFTNQLRKQGVGLMEAIEEAGEKRFLP
                     IILTTMTAIGGLMPIAWSNNPLISPLAIVMIGGLISSTFLSRIVTPVVYKLIPPKV"
BASE COUNT          947 a          799 c          835 g          992 t
ORIGIN      
        1 ccatcggttt ctcctaagaa aaccgttgaa gggacgcttg gggggattat tgctactatt
       61 gctctggcct ggccattatc gatgatcaat gatgcgttta cctatccaca atggttgatc
      121 gtagcaacca ttaccggagt cagcggaacc ataggcgatc tggttgaatc aaaactgaag
      181 cggcactacg gcatcaagga taccggaact taccttcccg gtcatggagg ttttctggac
      241 aggtttgatt ctttcctctt tgtagtttcc tttgtgtatc tttacaccca attaatctga
      301 caacctatgt ttccgattca cagggaagga cgagtgacca ttttgattgc tattcttttc
      361 agcatcctca tttggtggat attcaattca tttttacagg aattaatgtg gctgggttat
      421 gcactgataa tctttgtcct ggtcatggtg ttgcagtttt tccgaaaccc ccggcgacag
      481 gttccggcca tcgatgaaga cattgtgatt gctcctgctg atggcaaggt tgtcgttatc
      541 gaaaaagtac aggaagagga ggttttgaaa tgtaagtgta ttcaggtaag tatcttcatg
      601 tcgccactca atgtgcatgt caatcgcaat cctttgtccg gaataatcac ccacaatatt
      661 tatcatcccg gtaaatatct gactgcctgg aatcccaaat cttccactga gaatgagcgg
      721 acaacagtag tctatcagca tgacaaaggc accgtcctga tgcgtcaggt tgccggcgca
      781 cttgcccgac gaatcgtcaa ctacctgaag gaaggacagt ctgtgaggcg cggtgatgaa
      841 atgggcttca tcaaatttgg ctccagggtg gacctttact ttccacctga aaccgaaatt
      901 cttgtctccg tcaatcagat cgtcaaaggc aacatcaccc ggatagcccg actaaagtaa
      961 ttgcaggcga caggtccatg taatttcgat tggtagaaac ctgatttctt acacttttgg
     1021 cggtatcaac ttatacacga ccggcgtaac tatcctcgac agaaaggtag agctgatcaa
     1081 cccaccgatc ataacaatgg ccaggggcga aatgagcgga ttgttggacc atgcaatagg
     1141 catcaatcct ccgatagcag tcatcgttgt aaggatgatg ggcagaaatc gtttctcgcc
     1201 agcctcctcg atggcttcca tgagcccgac accttgtttg cgtagctggt tggtaaagtc
     1261 gaccagcaat atcgtgttct tgacctctat tccggcaaga gccacaatgc ccacagttgc
     1321 gacgaaggaa agtgaattgc ctgtgacaag tagtgcaagt actgcgccca caatgcccag
     1381 aggtattaca gatagaacaa tcagcgtgct tttgaaggta cggaattgca ataccaggac
     1441 agcgataaac aggaaaacgg tcaccagaat aatataacca aaacccttga atgattcctc
     1501 ccttccttct acttctcctc ccatggtaaa actgtatcct ttcggaaatt ttttctgctc
     1561 caatgctttc acgacatcat tgatgaccgc tgagttgaga tgtccttccc ggataaaggc
     1621 tgtcacagat acagtcctga ctttattcag gtgattgatg gtctgtgcgg atgtttcaaa
     1681 tccgatatcc gttatttgat ctattgggat agctcttccg tcagaagcat tgacaaaaag
     1741 attgcggaat acggataaat caggatagct ttctctgggt tttgtcacca caattgtata
     1801 atcattgttg ttcttttctt tatctgtata ggttccgatc ggtatacctg ccaaagccat
     1861 cctgacagtc tggcttatat tgatggtcgg aacgccgagg ctttgtgctt tttccttatc
     1921 gatatggatc cttatgtcag tcttgttatt ttttacggga ttgcttacat agatggcacc
     1981 cggattggct acaagcatat tttcgacttc agatgccaat tggcgcagtg tatccagatt
     2041 gtcgccaagt aagcgaactt cgatcggggc aatctgtgga attccttgtt caaagttgac
     2101 gaccttgact tcagctccgg gatagggagt ccaccggcga cgtagttcct tgatgatgtt
     2161 taatttctcg tccggtctaa tatccgggtg aagctgtacg aagatctggg cgaagtctgc
     2221 ttgctcatta cgttgtatta cgttgtaata tattctgggg ttacctttgc caatgttgga
     2281 ggaatagtat tttacctcag gcattttcgc caattcggct tctatttgtc ttgtgataga
     2341 atctgtgttg taaatgccgg actgaagggg tgcagtgata tcgatggtaa actgtggttt
     2401 ttctgaagcc ggaaacagac ttgtacctac gacagggatc aaaaataaag atcctacgaa
     2461 gatagataaa gcgataagaa ggcttaacac cggatgtctc aatgccacat ccagtaagcg
     2521 ggcatacgtg ccatggataa ctttctgaag tcccctgagg aaaatattac tatggtcacc
     2581 ttctgcaggt ttcaggattc tactggaaag gaaaggcaca atggtcaatg ccactatgag
     2641 tgaacccaga atagtaaata ttacagccaa aggcaggctg tggataaagt caccggatcc
     2701 ttcaggcatg aaagcaagag gcaaaaaggc gataatcaag gtcgccgtac accccaatac
     2761 ggccatgcct atctgacctg ttcccttgat ggtcgcgtct agacgggaat ggccctcgcg
     2821 aatccacctt tctatattct ccaccacgac aatactgtca tccaccagga ggccgagcgc
     2881 cactacaagg ccgacaatac tgagttgatt gagattatac ccgaaaagat tgagcaggat
     2941 gacaccgatg ccaattgata gggggatggc aatcatgaca accaaagagg cacggttgcc
     3001 caacggcaga agtgtaatca agaccagacc aatggcaatt agaaaatcca gccctaagtt
     3061 gtacaatcga tcgttgacat tgtcagcctg gtcaaaatga gcctgcatat caatattgga
     3121 cggcaaaccg gccctgaact tttctagtat gggcttgtag ctttcctgag ccagtgaaat
     3181 attttcaccg ggttttaagg cagcgttgac gaaaatacaa cgatggccgt tgagtcgggt
     3241 gatataagtg tccggcccat aatcataata cacatcagcg atatctttca gaaggatggt
     3301 atttactccg cgtccggtga taatggtatt tcggatttcc tgaagatttg aatagttgct
     3361 gttggtcttg acgttgaagg atttagtgcc ttcaatgata ttacctcccg gaatatttgc
     3421 ggattcactt tggagggcat tggctacgag caaagcagga atatgcagat gagccatctt
     3481 ttcaagatga aggtccacgc ggacaatgcg ttcagcggct ccgtttacct ttacattttt
     3541 tagctgagta actttctcca actcggcctg aag
//
LOCUS       DAOQQN010000337         3563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_12902_length_3563_cov_4.702680, whole genome shotgun sequence.
ACCESSION   DAOQQN010000337 DAOQQN010000000
VERSION     DAOQQN010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12902_length_3563_cov_4.702680"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="PLG24_11175"
     CDS             <1..1107
                     /locus_tag="PLG24_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25499.1"
                     /translation="KGMLADEDLGKNILLIKGAPMTMSGYNVTYSRDTIIDNNRTFTL
                     DFTRLDNNGKEKEKFSLNPNVVYERDFSKVAASNPSTLRGFFKDIYVHIASLPPEEID
                     REQAKAKEDSLKFETYWVSADDTVFTKRAYIKAGQLNFNPQHREYKPEPGDIAIGVNL
                     EVGNMKDDSIYKASPMVVLRDEVLMNFGAQINDLSTKIELHPDVFKPLFERNPEATRK
                     IVLKQGQKFSINDREFTFEKFKMSDKKEDKIAVFGELIDTEGKLVAEPKFEIIGNSSR
                     SEKVFMPSANALIAMTKIDPQEETATFEYNTFKGLGQKIPLRVSENSTRSDYIVLEAV
                     KFPGINFFWLGSMMMMAGLAWSILPKYRKKGRNV"
     gene            1100..1897
                     /locus_tag="PLG24_11180"
     CDS             1100..1897
                     /locus_tag="PLG24_11180"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HMZ25500.1"
                     /translation="MSEVPNRIQIVGTGNIAFHVASGLAKQQTVHSIRINARSETHHD
                     LFMSISPKIEFCINEKPDFTYPFTIIAVSDDAIGEVAEAWSGYPHLMVHTAGSVPIDV
                     LKEAGINNFGALYPLQTFSINRKINWKKIPIFINSNNEENLNNIYSVARLLSPNIIPI
                     TDSNRLALHIGAVIANNFTNHLFAIADKWLVSNDLKFEYLLPLIRETVDKLEEMSPLD
                     AQTGPAKRNDRRVINKHMGQLEGDQDLKELYLRLTESIFKFHHERGY"
     gene            2069..3163
                     /locus_tag="PLG24_11185"
     CDS             2069..3163
                     /locus_tag="PLG24_11185"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HMZ25501.1"
                     /translation="MKKEVKIGILAIVALFIVIFGLKFLKGQNVFSSKQTFYSDFADV
                     NKLTVGTPILIHGFQVGSVVDIYLKPSNPQLIEVEMQVNKDIKVPKSAIVEIREVGMM
                     GAKAIFLTYDIKALNDLAQSGDFLKGKTKGMLGSMLGDPAELQKYLSEIQQGAGGVMD
                     TIVDYFKRIDQSQGVGLALANLQHTIENLRLITAELNVLLHNSNSNLQATFGNLNSIT
                     GNINNKNKEIASLLENLNSISNQVNNAGLDNTIQNANTTITTLNNRMNELKTTLEGAD
                     KAINGISSVLTKVNNGEGTLGKLVNDNDLYLNLERTSKQLDLLMQDLRLNPKRYVNVS
                     VFGKKQKSYNVPEHDPANPILDSLETNKLK"
     gene            3170..>3563
                     /locus_tag="PLG24_11190"
     CDS             3170..>3563
                     /locus_tag="PLG24_11190"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ25502.1"
                     /translation="MLLEKPLRKIYYVMSPSLRLLVRRIVFLPKDLFRKKDEMMPPSG
                     LIYTGRGDFIRAGKYWLERFIREGGLKPEDSVLDIGSGIGRMAVPMAKYLTTGRYEGF
                     DAVRQGVDWCKSNIQRKYPNFNFIYADLD"
BASE COUNT         1202 a          699 c          696 g          966 t
ORIGIN      
        1 aaaggcatgc tggctgatga agatttaggt aaaaacatct tgctgattaa aggggctcca
       61 atgacaatga gcggttataa tgttacttat tcaagggata ccatcattga caacaatagg
      121 acatttacac ttgactttac aagactggat aataacggaa aggaaaaaga aaaattctcc
      181 cttaatccga atgttgttta tgaacgtgac ttctcgaagg tggctgccag caatccgtcc
      241 acactaagag gcttctttaa ggacatttac gttcatatag cttctttgcc acccgaagaa
      301 atcgaccggg aacaagctaa agctaaggaa gacagcctta aattcgaaac atattgggta
      361 agtgccgatg atactgtatt caccaagagg gcatatatca aggctggcca acttaatttc
      421 aacccacaac acagggaata caagccagaa cccggagaca tagcaatagg tgtcaacctg
      481 gaagtcggca acatgaagga tgattccatc tacaaagctt ctccaatggt tgtactgcgc
      541 gatgaagtgt tgatgaattt tggtgcacaa atcaacgatc tctccaccaa gattgaactg
      601 catcctgacg tgttcaaacc attatttgag cggaatccgg aagctaccag aaagattgtt
      661 ttaaaacaag gacaaaaatt ctctattaac gatcgtgaat tcactttcga gaaatttaaa
      721 atgtctgata agaaagaaga taaaatcgcc gtttttggtg aattaattga tactgaaggc
      781 aaactggtgg ctgagcctaa gtttgaaatc atcggtaatt cttctcgaag tgagaaggtt
      841 ttcatgccct ccgccaacgc actgattgct atgacaaaaa tagatccaca ggaagaaact
      901 gcaactttcg aatacaatac attcaaagga ctagggcaga aaattccatt gagagtatca
      961 gaaaattcta ctcgatcaga ctatattgtc cttgaagccg tcaaattccc gggaatcaac
     1021 tttttctggt tgggctcaat gatgatgatg gcaggccttg cctggagtat tcttccaaaa
     1081 tatagaaaaa aaggcagaaa tgtctgaagt acctaatagg attcagattg tcggaaccgg
     1141 aaatatcgcc ttccatgtag catcaggact ggctaagcaa caaactgttc actcaatacg
     1201 gataaatgcc cgctcagaga cacatcatga tttattcatg agcatatctc ctaaaattga
     1261 attttgtatc aacgaaaaac cggatttcac ttaccctttc acgatcattg ctgtttctga
     1321 tgatgccatt ggagaggtag cagaagcctg gtccggatat cctcacctga tggtgcatac
     1381 cgccggctcg gtaccaattg atgtattgaa agaggccggt attaataatt ttggtgcact
     1441 ctatccttta caaactttct ctatcaacag aaaaataaat tggaagaaaa tccccatctt
     1501 catcaattca aataacgaag aaaatcttaa taacatatac tctgttgcca ggctattgtc
     1561 cccaaatatc ataccgatta ctgattctaa tcgacttgcc ctccatatag gtgctgtaat
     1621 cgccaacaac tttaccaatc acttgtttgc aattgctgat aaatggcttg tatcaaatga
     1681 tctaaaattc gaatacctgc ttccattaat acgggaaacc gttgacaagc tggaagaaat
     1741 gtccccatta gatgctcaaa ccggacccgc aaaaagaaat gatcgaagag tcataaataa
     1801 gcacatggga caattggaag gagatcagga tttaaaggaa ttgtacctaa gactgactga
     1861 atctattttc aagtttcacc atgaacgagg ttactaatcc taatacttac tttacaaata
     1921 cattaatata ttatgtaaat ttaaaatgta tcataaaaga atccttctac aatcacaatt
     1981 ttaattagtt ttgcatttta ttgattcaaa tcagacacta acacatcaca taacttactg
     2041 attacctgaa aggtatgaac aatattttat gaaaaaagaa gtaaaaatcg gcattctggc
     2101 aatagttgca ctcttcattg tgatttttgg tctaaaattc ctcaaaggtc aaaatgtctt
     2161 ttcgtctaaa cagacctttt attcagattt tgcggatgta aataaactca ccgtcggtac
     2221 tcccatcttg attcatggct tccaggtagg atctgttgtg gatatttatc ttaaaccgag
     2281 taaccctcag cttattgaag tagagatgca ggtaaataaa gatatcaagg tacccaagtc
     2341 tgctatagta gaaattagag aggtaggtat gatgggcgcc aaagccattt tcctgactta
     2401 tgacatcaag gcactcaatg atcttgccca gagcggtgac ttcctgaaag gtaaaacaaa
     2461 agggatgctg ggttcaatgc ttggtgatcc ggcagagttg caaaagtatc tttccgaaat
     2521 ccaacaaggc gctggaggtg taatggatac gattgtagat tattttaaaa ggatcgacca
     2581 aagccagggt gttggccttg cattagccaa tcttcaacac accattgaaa atcttcggct
     2641 gataacagca gaactgaatg tcttgctcca caattcaaat tcaaaccttc aagctacttt
     2701 cggcaatttg aacagtatta ccggcaacat caacaataaa aataaggaaa tagcctcatt
     2761 attagaaaat ttaaacagta tttcaaatca agtcaataat gccggccttg acaatacaat
     2821 ccaaaacgcc aatactacaa tcaccacact aaacaatcgg atgaatgagc tgaagactac
     2881 attggaaggc gcggacaagg ctataaatgg catatcatcc gtccttacaa aggtaaataa
     2941 tggcgaggga acattgggga aattagtaaa tgataatgac ctgtatctca atctggaaag
     3001 aaccagcaaa caactggacc ttctgatgca ggatctcagg cttaacccaa agcgatatgt
     3061 caatgtcagc gtctttggta agaaacaaaa atcttataat gttccggaac acgatccggc
     3121 taatccgatt ctggattctt tagaaactaa caagcttaaa taagaagctt tgttgctcga
     3181 aaaaccttta cgaaagatat attatgtgat gagcccgtct ttgaggctcc tcgtaagacg
     3241 tatagtcttc ttaccgaagg atttattcag aaaaaaagac gaaatgatgc ctccctctgg
     3301 cttgatctac accggaagag gcgattttat tcgtgcggga aaatactggt tggaacgatt
     3361 tatacgtgaa ggaggcctga agccagaaga ttccgtacta gatattggaa gcggtatagg
     3421 cagaatggca gtaccgatgg caaaatatct tacaaccggt agatatgaag gttttgatgc
     3481 tgtgcgacaa ggcgtcgatt ggtgtaaatc aaatatccaa agaaaatacc ccaattttaa
     3541 ctttatttat gctgatttag aca
//
LOCUS       DAOQQN010000338         3547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_12997_length_3547_cov_5.457331, whole genome shotgun sequence.
ACCESSION   DAOQQN010000338 DAOQQN010000000
VERSION     DAOQQN010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12997_length_3547_cov_5.457331"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..1211)
                     /locus_tag="PLG24_11195"
     CDS             complement(237..1211)
                     /locus_tag="PLG24_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HMZ25503.1"
                     /translation="MELTELIEAYHRFTAENKYETNYPGLFEPIEYLLGLKSKKVRPL
                     LALIGYNLYNEDIANCLHTAHGLEIFHNFTLMHDDIMDEASIRRGQRTVHEKFGLNNA
                     ILSGDAMAILAYQYILKDSKPKNAIEVLTLFSKTAMEICIGQQMDMEFEQKEIISSEA
                     YIEMIRLKTAVFLGMALQIGAMIAGADLESTKPLYRYGENLGIAFQIQDDLLDLKGDS
                     FTVGKVKGGDILRKKKTILICKALEIASDQDSRSILDLLDNDTINAEEKVTTMTALLE
                     SLSVFTIVEQEINSYFNLAYKQLDMLSIKPHGKTLLISIANMLKSRKG"
     gene            complement(1317..2147)
                     /locus_tag="PLG24_11200"
     CDS             complement(1317..2147)
                     /locus_tag="PLG24_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25504.1"
                     /translation="MNFKLKIAGFAMLALFMASCNSNKKSVEADGTDLSNFKRVGSVD
                     TMQNKEVLPGNVASIDEYVRTTQKGMRSDLKDFQNDTKYKSIALGGKNNSILLTNITY
                     SSFEMNSLKVFFKDDHIIYAESRNQGKDGMDIYKYYFEGDKVTTTLKSKVEKTDDERG
                     KMNLKFEKIDNIPGRDAQIISLEKEAKTKFLSEAPFKCRVYKEGEGYKAITCYNGLEL
                     KVVDPNGFIVKSFRDHDLGENNKFQFIQVTGNINEKEGNITVSKIIYQGMGGANTDCF
                     "
     gene            complement(2165..2605)
                     /locus_tag="PLG24_11205"
     CDS             complement(2165..2605)
                     /locus_tag="PLG24_11205"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HMZ25505.1"
                     /translation="MKNILFLAGILMLISCNTSKKAKNSNDMFLTGTKWTVIKIRESM
                     ATSTEAVRYIELSLPNKKGEGTISGNVGCNNFRGNYKSQKGTSNIQFTQLSSTKMTCQ
                     NIDQETTIMSFLEKVNSYEISGSILKLYAGGELLLTLEGVNITQ"
     gene            complement(2776..3429)
                     /locus_tag="PLG24_11210"
     CDS             complement(2776..3429)
                     /locus_tag="PLG24_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HMZ25506.1"
                     /translation="MLFLVYMGSINNIIFDLGNVLLDIDYSLTADRLSDVTGLDINRD
                     LMEYGEIFKKFETGKIPYVVFFNHLTKISPKKAIINDMLPAWNAMLIGMQPEIPALLD
                     SLSMNYSLFILSNTNEIHIQWLDKYLKQENIYEKWYHDWFRQIFYSHQLGLRKPEKQC
                     FEEVLIQSNILPEATLFVDDNVANVNGSIEAGICGVLYDKSQKSLVDFLRNDAQLIV"
BASE COUNT          985 a          694 c          669 g         1199 t
ORIGIN      
        1 acatatagtt atgtcgatgt tgaatatatg gctaccatgg taggcgaata gatttactat
       61 tgttcgcggc ttgttttgga cacttcatta tcctgcaagt aaaagggtgt tgtatccgaa
      121 tacctgaacc atgattatac aggctataaa agttattggc tatctgatgc ccttatacgg
      181 agaatagccg aacaaatttg aaatatctct ttctgtattt ggtcgctgca attatcttac
      241 cccttcctgg acttcagcat attggcgatg cttatgagca gtgtttttcc gtgtggtttg
      301 atactaagca tatcaagctg tttgtatgca aggttgaaat acgaattaat ttcttgctct
      361 acaatcgtga aaacgctgag tgattccaac aaagctgtca tagtggtgac tttttcttca
      421 gcatttatag tatcgttatc caatagatcg agaatactcc gactatcctg atctgacgct
      481 atttccagtg ctttgcaaat caggatggtt ttctttttgc gaagtatgtc gccgcctttt
      541 acctttccta ctgtaaaact atcacctttc aggtccagca agtcatcctg aatttgaaag
      601 gctataccga ggttctcacc atatcggtaa agcggtttag ttgattctaa atccgctcct
      661 gcaatcatgg cgccgatttg caatgccatt ccaaggaata cggccgtttt caaccttatc
      721 atttcgatgt atgcttctga agagatgatt tctttttgtt cgaattccat atccatctgt
      781 tggccgatac agatctccat agctgttttg ctgaaaagag tcaatacttc tattgcattt
      841 tttggcttac tatccttgag tatatattgg taagccagaa tggccattgc atcaccggaa
      901 agtatggcat tattgaggcc gaatttctca tgtactgtcc tctgaccgcg tctgatggat
      961 gcttcgtcca taatgtcatc atgcatgagc gtgaaattat gaaagatctc cagaccatgt
     1021 gctgtatgca ggcaattggc aatgtcttca ttgtaaaggt tgtaaccgat gagtgccagc
     1081 aagggtctta cctttttact tttcagtcct aaaagatatt caataggctc gaatagtccg
     1141 ggataatttg tttcgtattt attttcggca gtaaaccgat gataggcttc aattaattcc
     1201 gttaattcca tggatgtaaa ataaataaaa aaaggccgaa aatacttcgg ccttattatt
     1261 ttaattgccc ttttttcttt cgttataaag gcagaataga gtattcatga ggagtatcag
     1321 aaacaatctg tattagcacc tcccataccc tggtaaataa ttttggaaac agtaatgttt
     1381 ccttctttct cattgatatt acctgtgacc tgaataaatt gaaatttgtt gttttcaccc
     1441 aggtcatgat cgcggaagga ctttacgata aaaccgttag gatcgaccac ttttagttcc
     1501 aatccattat agcatgtaat cgctttgtat ccttcacctt ctttatacac tctgcatttg
     1561 aaaggagctt cagaaaggaa tttagttttt gcttcttttt caaggctgat gatctgggca
     1621 tccctgcctg ggatgttgtc gatcttctca aactttaagt tcatcttgcc gcgctcatca
     1681 tcagtctttt ctactttgct tttcaaggtt gtagtaactt tatcaccttc aaaatagtat
     1741 ttgtagatgt ccataccatc cttgccctgg tttcttgatt cagcataaat aatgtgatcg
     1801 tccttaaaga aaactttcag ggagttcatt tcaaagctgg aatatgtgat atttgtcaga
     1861 aggatggaat tgtttttacc tccgagagca attgacttat actttgtatc gttctgaaaa
     1921 tctttcagat cactgcgcat tcccttttga gtagtgcgaa catattcatc gatagaagcg
     1981 acgttgcccg gaaggacttc cttattctgc atggtatcca cagaaccaac tcttttgaag
     2041 ttgctcaggt cggttccatc ggcttcaaca ctttttttat tggagttgca agatgccatg
     2101 aataaggcaa gcattgcaaa tccggcaatt tttaatttaa aattcatgag aaatatttaa
     2161 atgattattg agtaatattt acaccttcga gagtcagtag taattcacca ccggcatata
     2221 acttcaggat agagcctgaa atctcatatg agttaacctt ttccagaaaa gacattattg
     2281 tcgtttcctg gtctatattc tggcaagtca tcttggtgga agaaagttgg gtaaattgga
     2341 tattcgaagt acctttctgg ctcttgtagt taccccggaa attattacaa cctacattgc
     2401 cggatatggt accttctccc tttttattgg gtagtgatag ttcgatatac cttacagctt
     2461 ctgtactggt agccatagat tctcttattt tgatgaccgt ccatttagta cctgtcaaaa
     2521 acatgtcgtt gctgtttttt gctttcttac ttgtattgca tgaaataagc attagaatcc
     2581 ccgctaaaaa aagaatgttt ttcatatcgt ttttattttt tcaaaagttg gcgcaaagtt
     2641 gagaagattt ctacagatta tgaaatatat tctatatttt tattatacaa tgaaatccag
     2701 gccacaaggg cattctgtct atatttgctt aatcttaaca aggccgggtc taatttatta
     2761 acttcagaaa tcggctcaga ctatgagttg tgcatcattt ctgagaaaat caaccagact
     2821 tttttgagac ttgtcatata gcactccaca aatgccggcc tctatcgacc cattgacatt
     2881 tgccacatta tcatcaacaa ataacgttgc ttcaggtaaa atattggatt gaatcagaac
     2941 ctcttcaaaa cattgtttct caggttttct taggccgagt tggtgggaat agaaaatttg
     3001 ccggaaccag tcgtggtacc atttttcgta gatgttttcc tgtttcagat atttatccag
     3061 ccattgaata tgaatttcgt tggtgttgct aaggataaaa agggaataat tcattgacaa
     3121 gctgtccagt aaagctggaa tctccggctg cattcctatg agcatggcat tccaggcagg
     3181 cagcatgtca ttgataatcg cttttttcgg agatatcttc gtcagatggt tgaagaaaac
     3241 cacataggga attttcccag tttcgaattt cttgaaaatt tccccgtatt ccatcagatc
     3301 acgattgata tccaggcctg taacatccga taatctgtca gctgtcaggg aataatcaat
     3361 atccagcagt acattgccaa ggtcaaaaat gatattatta atgcttccca tatatactaa
     3421 gaacaacaca agttacggat attcaaaaaa gaaaaaaggg ttttgaaatc atgattcaaa
     3481 acccttttag gggtgggccc accaggactt gaacctggga ccccctgatt atgagtcagg
     3541 tgctact
//
LOCUS       DAOQQN010000339         3547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_12998_length_3547_cov_4.576174, whole genome shotgun sequence.
ACCESSION   DAOQQN010000339 DAOQQN010000000
VERSION     DAOQQN010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12998_length_3547_cov_4.576174"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PLG24_11215"
     CDS             complement(<1..361)
                     /locus_tag="PLG24_11215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ25507.1"
                     /translation="MVQESTFCVVTPVFNDWDCFERLYVELKAMFAEQGHRFMVLAVN
                     DGSTLPFPEHFKVIQNIDIEDRVSWCEECILVLDLQRNLGHQKAIAIGISFVERHITP
                     DYCIVMDSDGEDRPSDIP"
     gene            complement(740..1324)
                     /locus_tag="PLG24_11220"
     CDS             complement(740..1324)
                     /locus_tag="PLG24_11220"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HMZ25508.1"
                     /translation="MINLILFGPPGSGKGTQAVRIAEKYNLVHISTGDLFRYELNNMT
                     ELGKLAKSYMDKGQLVPDEVTVDMLKSKMDRHPDANGFIFDGFPRTLNQADALMSLFK
                     ARKTSLTAMLALEVDEDEIVRRIKMRGETSGRADDNDEDIIRNRFAVYKNETQPVFNY
                     FKNLGLATSINGIGDVDKIYHLLVNRIDRALNRL"
     gene            complement(1660..2916)
                     /locus_tag="PLG24_11225"
     CDS             complement(1660..2916)
                     /locus_tag="PLG24_11225"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMZ25509.1"
                     /translation="MKGLRIHKPKPLSIPSVEKVVLNNGLKLYFLEGGAQEVVKFEWC
                     FKAGRSSEKSRLASVLCHSLIKEGCEGYSGFQIAEHFDYFGSSLNHQAGFDYCSFSFY
                     CLNRFFDPLLDMFLKVISKPSFPENELSLLKSKHISRLRQERDDADFSAYKILTEKIF
                     GTEHPYGYNSTEELVHHMHTDLLRLHFESNYLTSEDFVMITGRLMPGSKQELTDRLSS
                     VKLHSPPENNTRLVEKSEAEKYLDNSFSKNQCAFKLGQRTIKRDHEDFPAFYIFNHLL
                     GGFFNARLSRIIREQKGLTYNILSTHDTYVHDGIFYISAESSWKNLPELRNEVYRQLD
                     NVLKKPPSKAELERVRAFLIGVLITSFDGVFATADTFRSCLVEEVDLISLQPLMDAIV
                     KMSAEEVFERSHRHLDPARMIEVVTG"
     gene            complement(2913..>3547)
                     /locus_tag="PLG24_11230"
     CDS             complement(2913..>3547)
                     /locus_tag="PLG24_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25510.1"
                     /translation="FYLNMFSRKTFLLGLMVLTFAACKRDSNTDEVNTDVVELQGVKT
                     STPDTSFGFSKKLATENLHISIKTKDFEGGKMINLIISQRDGGNINKKIKIDGEIIDA
                     FTADLNGDGKDEVYIFNQGEGSGSYGNLYGYQAETSGLDSISMGDLPAQYRDKYMGHD
                     SFAIDGKQLLRFIPLYNEIDPTCCPTGGKATIKYKLANVKGKLVLVAEQK"
BASE COUNT         1071 a          796 c          629 g         1051 t
ORIGIN      
        1 gtggaatgtc tgaaggtctg tcttctccat ctgaatccat tacaatacag taatccggag
       61 taatatgtct ttcaacgaag gagatgccaa tagcgatggc cttctggtga ccaagattcc
      121 gttgaagatc aagaactaaa atgcattctt cacaccagga gactctatcc tcaatgtcta
      181 tgttttgtat taccttgaaa tgctcaggga aaggcagtgt gctgccatca ttgacagcca
      241 ataccataaa cctatggcct tgttcggcaa acattgcttt caactcaaca tacagtcgtt
      301 caaagcaatc ccagtcattg aagacgggcg taacaacaca aaatgtactt tcctgtacca
      361 tctatcctta aaaaaaagca caaaataagc aaaactctcg gattgacgtc atagaaatgc
      421 caaaggcata aggtggaaat attgcaagtc aaagaccggg tcggagccac ttgtcactga
      481 aattatctgt cggatgatga taaaaccact atagaaaaag ttgctgaatg aaactggcca
      541 ggacaacgaa tgcactatcc tacattcaac ataatgcatc cattaattac tataaacttg
      601 gtgtatcctt aaaatgtttg ttgggaaagc tttattatcc aatgttgata aaactttttc
      661 aaatttgtct tcctaaattc tagttagggc atcaaatata aattcacccc attaagccca
      721 acaatgtcgg aaacaataat tacaacctat ttagtgcccg atctatcctg ttcaccagca
      781 aatggtaaat cttatccacg tcacctatcc cattgatgga ggtggccagt ccgagattct
      841 tgaaataatt aaacaccggt tgagtttcat ttttataaac agcgaaccgg tttctgataa
      901 tgtcctcatc gttgtcatct gcgcgacccg atgtctcccc acgcatcttt atcctccgaa
      961 ctatctcatc ctcatcaact tccaaagcaa gcatagcggt caaagatgtt tttctcgctt
     1021 taaacaatga cataagggca tcagcctgat tcagggtacg cggaaatccg tcaaaaatga
     1081 agccattggc atccgggtgc ctgtccatct tacttttcag catatcaaca gttacttcat
     1141 ccgggaccaa ctgaccttta tccatataac tctttgccaa cttgcccaat tctgtcatgt
     1201 tgttcaactc atatctgaat aaatctccgg tggagatatg cacaaggttg tatttttccg
     1261 caattcgcac tgcctgtgtc cctttgccgg aacctggagg accaaaaagt atcaaattta
     1321 tcatgataac aggatgttat ttaaagccga aaatagaaaa attatttgaa agtttacaat
     1381 tctttgaaaa tttagaatat atcacaaatt gcatctcact tatttcagaa agaacagttt
     1441 ggcagctttt catcagctga tgtgcaatca atcctgtgac ccaattgatg ctattaattg
     1501 gcaagttcgg ttgaaggaag gtccttaata aatgcgtgtt tggcaattgc ttgtagcatt
     1561 tccattagtt tagtttgtga tcaagaaaaa aatctatcat tctccaaatt aaaatttgaa
     1621 aatttatgtt taaccgggtt cccttgatta tcatattatt caaccagtca caacctcgat
     1681 catcctcgcc ggatcaagat gtcgatgtga gcgttcaaaa acttcctctg ccgacatttt
     1741 tacaatggca tccatcaatg gttgcaatga tataaggtca acttcctcga ccaggcacga
     1801 acggaatgta tcagccgtgg caaatactcc atcaaaagat gtgataagaa ctccgatgag
     1861 aaatgcccga acacgctcca attcagcctt ggaaggtggt ttcttaagga cattatccag
     1921 ctgtctatat acctcattgc gcaattccgg caagtttttc catgatgact cggccgatat
     1981 atagaaaatt ccatcatgaa catatgtatc gtgggtactc aaaatattgt aagtcaaccc
     2041 cttttgttct cgaattatcc gactgagtct tgcattgaag aagcctccta gcaaatgatt
     2101 aaaaatgtaa aaggcaggga agtcctcatg atctctttta attgtccgct gtcctagttt
     2161 aaaggcacat tggttctttg aaaaagaatt atccaaatat ttttcagcct cacttttttc
     2221 aaccaacctt gtgttattct ccggcggcga gtgaagctta actgagctca acctgtcagt
     2281 aagttcttgc tttgatccgg gcatcagcct tccggtaatc atcacaaagt cttctgatgt
     2341 taaataattc gactcaaaat gtagtctaag caagtcagtg tgcatatggt gcaccaactc
     2401 ctctgtactg ttgtacccat aaggatgttc ggtaccgaaa atcttttcag tcagtatctt
     2461 ataggcagaa aagtctgcat cgtccctctc ctggcgcaat ctgctaatat gcttactttt
     2521 aagtagagat aattcattct ccggaaagga aggttttgat atcactttta gaaacatgtc
     2581 cagcaatgga tcaaaaaacc tattcagaca atagaaagaa aatgaacaat aatcaaagcc
     2641 tgcctggtga ttgagtgaag aaccaaagta atcgaaatgc tcggcaatct gaaatccgga
     2701 ataaccttca cagccttcct tgatcaggct atggcataac actgaagcca accggctttt
     2761 ttctgaagaa cgtcctgcct taaagcacca ttcaaattta acaacctctt gtgcccctcc
     2821 ttccagaaaa tatagcttca gcccgttgtt caggacaact ttctcaactg aaggaatgga
     2881 aagaggcttg ggtttgtgga ttctcagtcc tttcattttt gctctgctac gagcacaagt
     2941 tttcctttga catttgccaa cttgtatttg atggtcgctt tccctcccgt tggacaacaa
     3001 gtaggatcga tctcattata aagcggtata aatcttagca attgcttccc atcaatggcg
     3061 aaactatcgt ggcccatata tttatcccgg tattgggccg gcaaatcacc catggaaatg
     3121 gaatcaagtc cggatgtttc agcttgataa ccatagagat taccataact tccactgcct
     3181 tcaccctgat tgaagatata tacctcatcc ttaccatcac cattcaaatc agctgtaaaa
     3241 gcatcgatga tctcaccatc gatcttaatc tttttattga tattgcctcc atccctctga
     3301 ctgatgataa gattgatcat cttgccccct tcaaaatcct tagtcttaat ggaaatatgc
     3361 agattttcag tggcaagttt tttggaaaaa ccaaaggaag tatccggagt tgatgttttc
     3421 accccttgta attccaccac atcggtattc acttcatccg tattgctgtc tctcttgcag
     3481 gcagcaaaag ttaaaaccat cagccccaaa agaaaggtct tcctgctgaa catattaaga
     3541 taaaaat
//
LOCUS       DAOQQN010000340         3542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_13030_length_3542_cov_4.271580, whole genome shotgun sequence.
ACCESSION   DAOQQN010000340 DAOQQN010000000
VERSION     DAOQQN010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13030_length_3542_cov_4.271580"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="PLG24_11235"
     CDS             complement(<1..814)
                     /locus_tag="PLG24_11235"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HMZ25511.1"
                     /translation="MKSFLFLIFGCIIICNNLFSQSNQFCGFDARHELLLQSDSSYKE
                     NFQLWEEKLYNELKQGSPLQIRQTYTIPVVVHIITPPGTPIGTGNNLTDTEVEAGLAL
                     LNDAFANRGAFHTPEGQDIGIQFCLAKRTPDGQPTNGITRHESNLVADAACSSYGTSM
                     DNDAAIKKIVTWDCRQYLNIWLVTDLYNSSFGCSLAGFAYFPGAPCTLDGIIQESRYW
                     TTVGGTVVTAHETGHYFGLYHTFQGGCKNDDCLIDGDKVCDTPPDGSPSFAPC"
     gene            complement(1319..>3542)
                     /locus_tag="PLG24_11240"
     CDS             complement(1319..>3542)
                     /locus_tag="PLG24_11240"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMZ25512.1"
                     /translation="LNYRSYFIPKLCTKDAAGNYWIGTESNVQKILAYQYIIPVSADN
                     YMNETWAITQAKNGNLWMASYGSGLRIFDGQSLKKPPTGFYSFDKAHFDDVAIQCDDG
                     SMLFNLDYNRTDEKHLLLSGIIKIKGQKWDFIPSERPGIFFGRDRKGHLMRGTIETGL
                     FIYKDDSDLSANNLFLLIDSTKGLATGNIVSALQDRYGRYWMGRASQGLSMYDPDKNK
                     IANWMTYDDVNNPSVVSIQEDSRGNLWFGCFDGLWYLENSPDIEAGFPITKRRIKIES
                     EYMGSSTNFITKIIDDSTLLAGNAFGYFLIDLNAFYKNKKNYCEFKGIFNQEYHNYSG
                     GAAIQNGVFRSADGCYWILTDNGIVRHDPAKYVYSKSERTVEIDSIRAGGKLYSEGFK
                     NIVMGYRSPHITFYFSSPTDSLLYDNLFYQYRLNNGPWSNLTRDGYANFSELNAGHYT
                     FSVRTVVEGHRSDVKTISFLINPPWWMKWQVWLATLILILGVGLFFYQKQKKIHHQQL
                     MLSQKETEVEIINKEKNLLRVEAIVNQLNPHFINNVLQWLQIRVDDDNEAVKVIGRLA
                     QNINTVFRNSRMKISFHTLENELQLASNYLYIQKVRFGDLLKLDIVDLNTVEELKGIM
                     VPIMVIQIHVENAVEHGIRNNEDGFGTVTIYVADEGDYIRIIVRDDGVGREAASRLGS
                     KGTQNGVNMLSELAKIYNKNNELRISQQYRDHIFTHPDGRGYGTEVVIRIPKKYNYVV
                     "
BASE COUNT          936 a          755 c          752 g         1099 t
ORIGIN      
        1 tacagggtgc aaacgaggga ctgccatcgg gtggagtatc acagactttg tcgccgtcaa
       61 tgaggcaatc atcattttta caccctccct gaaaagtgtg gtaaaggcca aaataatgac
      121 ccgtttcatg ggctgtgaca accgtgccac caaccgttgt ccagtaccgg ctttcctgaa
      181 tgatgccatc cagggtacat ggtgctccgg gaaagtaggc aaagcctgcc agggaacatc
      241 caaaggagga attatacaag tccgttacaa gccaaatatt caggtattgc ctgcagtccc
      301 atgtgacaat ctttttgatg gcagcgtcat tgtccatact tgttccatat gaagagcagg
      361 cggcatctgc caccagattg ctctcatgtc gggtaatgcc attggtcggc tgaccatcag
      421 gtgtgcgttt cgccagacaa aactgtattc ctatatcttg tccttcaggt gtatgaaagg
      481 cacctctatt ggcaaatgcg tcattgagca gggccagacc ggcttccact tccgtatcgg
      541 tgaggttatt tccggtacca atcggagttc cgggaggtgt gatgatatgt accacgactg
      601 ggatggtgta ggtttgcctt atttgtagag gcgatccttg ttttaattcg ttatagagtt
      661 tttcctccca aagttgaaag ttttccttgt aggatgaatc cgattgcaat aataattcgt
      721 ggcgggcatc aaatccgcag aattggttag attgagaaaa aagattattg caaatgatta
      781 tacacccaaa tatcaggaat aaaaaagatt tcatacgaat ggttttaaaa tatgcttctt
      841 attgctaaat cacaagctat ttttacaaaa tcggtttcat tgttatgctt ttgtacagct
      901 tttaatgtat gtaaacctga tctacacttc atcttaataa tttttatatt ctttatggat
      961 gatgcgataa tttcttgtaa atacttcgat gaaatatagc ctttacttga caataaagaa
     1021 atcacccaat tccgacaaag caaggctgta gtgtaaaggc cggattcgac agatgcttct
     1081 ttagtattgc tatttcgata aggttgaagg tcatacatgg tgaagtaaaa ttatttcacc
     1141 cgcaatttat tatatatata catacaaaat ccgggtcgaa cagtgaacac ggtattttcg
     1201 ctaatgaaac gggtatttat gggtatgaaa aggtttgagg gcgaaaattt tctcttgctt
     1261 tacctggttt taagggttgt acaacgagcc ttacaaggca tacattttcc acgtctcact
     1321 acaccacata gttgtacttc tttggaatcc tgatcacgac ttccgtgccg taccctctgc
     1381 catcgggatg ggtgaagata tgatccctgt attgttgtga aatccggagt tcattgttct
     1441 tattgtaaat cttggcaagc tctgaaagca tgtttacccc attttgggtt ccttttgatc
     1501 ccaggcggga tgccgcctca cgccctacac catcatccct gacgatgata cggatgtaat
     1561 caccctcatc cgcaacatat attgtgaccg tcccgaaccc gtcctcattg ttgcgaatgc
     1621 cgtgttctac tgcattttcc acgtgaatct ggataaccat gataggtacc ataataccct
     1681 taagttcttc cactgtattc aggtcaacga tatcgagctt tagcaggtcg ccaaatcgca
     1741 ctttctgtat atacaggtag ttggatgcca gctgaagctc attttccagt gtatggaagc
     1801 ttatcttcat gcggctgtta cggaatacgg tattgatgtt ttgggcaagg cgaccaatga
     1861 ctttgacggc ttcattatcg tcatcgactc ttatttgcag ccattgtaag acgttgttga
     1921 taaagtgcgg attgagctga ttgacgatgg cttctacccg gagcaggttt ttctccttgt
     1981 taatgatttc tacttccgtt tccttttgtg acagcatcag ttgctggtga tgtattttct
     2041 tttgcttttg gtaaaagaat aatccgacac caaggattaa tatcaaagtg gccagccaga
     2101 cctgccattt catccaccat ggagggttga taaggaatga aatagttttg acatccgacc
     2161 ggtgaccttc cactacggtg cgaacagaga aagtataatg tccggcattg agttcggaaa
     2221 agtttgcata cccatctctg gtcaggttgc tccacggacc attgttgagc cggtattgat
     2281 aaaaaaggtt gtcatacagg aggctgtcgg tgggtgaact gaaataaaat gtgatgtgcg
     2341 gacttcggta acccatcaca atgtttttga agccttcgga ataaagtttt cctcctgcac
     2401 ggatgctatc tatttcgacc gtacgctctg atttggaata aacatattta gcagggtcgt
     2461 gccggactat tccgttatcg gtaagtatcc aataacagcc gtcagcagag cggaaaaccc
     2521 cattctggat tgcggctcct ccggaataat tgtgatactc ctgattgaat atgcccttaa
     2581 actcgcagta gttctttttg tttttgtaaa atgcattcaa atcaattaaa aaataaccaa
     2641 atgcattgcc ggcaagcaaa gttgagtcat ctattatttt tgtaataaag ttggttgagc
     2701 ttcccatgta ttcgctttct atcttgatac gacgctttgt tattgggaag cctgcctcta
     2761 tatccgggct attttccaaa taccaaagtc cgtcaaagca accaaaccaa aggttgcctc
     2821 gtgaatcttc ctgtattgac actacacttg gattatttac gtcgtcgtat gtcatccagt
     2881 tggctatttt attcttatcg gggtcataca tagataggcc ctgggatgcc cgccccatcc
     2941 agtaacgccc atatctatcc tgaagtgctg acactatatt tcccgtcgct aatcctttcg
     3001 ttgaatctat taggagaaac agattattcg cagacaaatc tgaatcgtcc ttatatataa
     3061 ataggccggt ctcaattgta cctcgcatca aatgtccttt tctgtcccga ccaaaaaaga
     3121 tacctggtcg ctcagaagga atgaaatccc atttttgtcc tttgattttg atgatacccg
     3181 aaagtaatag atgtttttcg tcagttctgt tatagtcaag attgaaaagc attgatccgt
     3241 catcgcattg aattgccacg tcgtcaaagt gagccttatc aaaagagtaa aatcctgtag
     3301 gtggcttctt taggctctgt ccgtcaaata ttcttaggcc actaccatag ctcgccatcc
     3361 aaaggttgcc gtttttagcc tgtgttattg cccaggtctc gttcatatag ttgtcggccg
     3421 aaaccgggat gatatattga taggcgagta ttttctgaac attactttct gttcctatcc
     3481 agtagtttcc tgctgcatct ttggtacaaa gctttggtat aaaataagaa cggtaattga
     3541 gg
//
LOCUS       DAOQQN010000341         3540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_13047_length_3540_cov_5.392826, whole genome shotgun sequence.
ACCESSION   DAOQQN010000341 DAOQQN010000000
VERSION     DAOQQN010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13047_length_3540_cov_5.392826"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1904
                     /locus_tag="PLG24_11245"
     CDS             <1..1904
                     /locus_tag="PLG24_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HMZ25513.1"
                     /translation="FRDVTYEIREPEISIFLSDSWNKSVLKRGKVVSDLNYQLEKNRI
                     SDILFNNGYYFFDPSLIGPLIADSIGHEVNVAFRMNETDDRKVVQRYKIGRVNIHSDF
                     DQNYSKLYNLDTTINNYRFLQISPRFRVKPSTILRYIYLKSGDYYSKANFTRTYRQLN
                     RLGLFRYINVNQTIDDIDTTLLNVDLYLPPAKTLDISYNLDFGYVNLRTSAVAIPATI
                     LGQVGASVIKRNLFNGGEALRTNASAGYETSFNKAVNRIDLKLHNSLSFPRFMDFLGI
                     VSGLKRVKTSKGPLLPKSFIETLEDRVTTNITMDFEYNNFKDWYKYYNLNGSYGFDVI
                     QDNEHQYRWNHLGINYFKPTVFSLYSQVLENNYFLKQSFENPRLFTGLLFRDLSFIYK
                     TADDNYGQNRKFYVNAELSGFEINLINRLINPSTNWKLFDNVEFSKFFKIYTEVSSTK
                     VLFGRVLGAAKIGLGVSHSFGSSTTVPYLKQFEIGGPFSLRAFPIRKIGPGSYIDPVQ
                     QEKFPNGPYYQTGDMKIEYLSELRFPLFYIFKGAIFLDAGNIWSISKSDDNRPGSRFR
                     FKDKPWNQLAVGTGVGLRLDLSFFVLRLDMGSALKYPYKINGSYNPYSSFKDRLRNLE
                     YNLALNYPF"
     gene            complement(1901..>3540)
                     /locus_tag="PLG24_11250"
     CDS             complement(1901..>3540)
                     /locus_tag="PLG24_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25514.1"
                     /translation="LKYHFNLDKVYEEANDTLYKIDYWPRNSGYNCFKGMIIISANDW
                     AVQKITMKNTFMDLYPFVLYQEYWRVNNQWFPYKFITEVKLPSAFGKEHPFLVKQTNI
                     FDKITFLPIPIEPSKINKTEYQTGALPSIDSMRMMPLNRQDSTAYVFGDVLLRSGPSG
                     YILQNMQLILNGQLPIGPLAIDMLSIYRKNLFEGNRFGLSVHSSMKLMPHLGLEAYFA
                     YGTHDNALKYGGSVAWYFDKLRTSNIKYTWNKDVEGNRLFRFRNQWFNQYYSNLFVEI
                     DKHEFSYFKTSASHSIKLATTRETIDPYFEYDFKGINPGESGEYINSEISASYQYIKG
                     RQINFFNTYFIVRNPSYPIFNVSVKSGLKGIAGGNFSYGAIEANFNKIFRWAIIGNTS
                     LTIQGGYIMGRPPIFKLFNAPGARILSMTISTPYSFQTMPPNHYFSTRYLHIFLQQTI
                     RRLYATKFSAPELFVSYNAGWGRLDNMSDHSGVTLKDYHDVFQEVGLGLNSFIRIPIA
                     DWFAFGINAGAYYNLNGGLPLEFGKNYNIKTGFGFLF"
BASE COUNT         1022 a          669 c          736 g         1113 t
ORIGIN      
        1 tctttcgcga tgtcacttat gagatcaggg agccggaaat ttctattttt ctaagtgatt
       61 cctggaataa atctgtcctg aagagaggaa aggtggtgtc cgacctcaat taccaattag
      121 aaaaaaacag aatatccgac attcttttca acaatggata ctattttttt gatccgagcc
      181 ttatcggtcc cttgattgca gacagtatcg gccacgaggt caatgtggca ttcagaatga
      241 atgagacgga cgacaggaag gttgtccaaa gatataagat tggaagggtc aatatacata
      301 gtgactttga tcagaactac agtaaattgt ataatctcga tacaaccatc aataattacc
      361 gcttcttgca gataagtccc aggtttcggg ttaagccgtc cactattctg agatacattt
      421 atttgaagag tggtgattat tattccaaag ctaattttac cagaacttac cggcaattga
      481 accgacttgg gctttttcgt tatatcaatg tcaatcagac gattgatgac attgatacaa
      541 cattgctcaa tgttgatcta tatctcccgc ctgccaaaac gcttgatata tcttacaacc
      601 tggatttcgg ctatgtcaac ttaagaactt ctgccgttgc tattcccgcg acaattttag
      661 ggcaggtagg agcatccgtc attaaaagaa acctgttcaa tggaggcgaa gctctccgga
      721 caaatgccag tgcaggatat gagacaagtt tcaataaagc tgtaaacaga atagatctca
      781 aattgcacaa tagtctgtcc tttccacggt tcatggactt tttggggata gtttccgggt
      841 tgaagagggt caaaacaagt aaaggccctc tattgccaaa gagttttatt gaaacgcttg
      901 aagatcgggt gactaccaac attaccatgg attttgaata caataatttc aaagattggt
      961 ataagtatta caatttaaat ggttcttatg gatttgatgt tatccaggac aacgagcatc
     1021 aatacagatg gaatcatctg ggtatcaatt atttcaagcc aacagtgttc agcctttata
     1081 gtcaggtcct tgagaataat tattttttaa aacagagctt tgaaaatcct cgtttattca
     1141 ccgggttatt gttcagggat ctttccttca tctataagac agctgatgac aattatggtc
     1201 agaacaggaa attttatgtc aatgcagagt tgtccggatt tgaaatcaac ctgattaacc
     1261 gcttgattaa cccttcaacc aattggaaac tgtttgacaa tgtcgagttt tccaagtttt
     1321 ttaaaattta taccgaggta tcttcgacaa aagtgttgtt tggccgggtt ttaggggcag
     1381 ccaagatcgg actgggcgtt agccattcat ttgggagcag cacgacagtg ccttatttga
     1441 aacaatttga gattggtgga cctttcagtt tacgggcatt tcccatccga aagattgggc
     1501 cgggcagcta cattgatccg gtgcagcagg aaaaatttcc caatggacca tattatcaga
     1561 ccggggatat gaaaatagaa tatttgtctg aattaagatt tccgttattt tatattttta
     1621 aaggtgcaat ttttttggat gccggcaata tttggtctat cagcaaaagc gatgacaaca
     1681 gaccgggatc tcgttttcgc ttcaaagaca agccatggaa tcaacttgcg gtcggaacag
     1741 gagtgggttt gaggcttgac ctgagttttt ttgtactcag attggacatg gggtctgcct
     1801 taaagtatcc ttataagatt aacggtagct ataatcccta ttcttccttc aaggacaggc
     1861 ttaggaatct ggaatataac cttgccctga attatccgtt ctaaaataga aagccaaatc
     1921 ctgttttgat gttgtagttt ttgccgaatt caagtggcag gccaccattt aaattgtaat
     1981 aagctccggc attgatccca aatgcaaacc aatcagcaat tggaatccta atgaatgaat
     2041 tgagacctaa accaacttcc tgaaatacat catgatagtc cttcagagtg actccactat
     2101 ggtcactcat gttgtccagc cgaccccagc ctgcattgta acttacgaat aattccggtg
     2161 cggagaattt ggtggcataa agtcttctga tggtttgttg taggaatata tgtaaatacc
     2221 tggtgctgaa gtaatggttg ggaggcattg tctgaaacga ataaggtgtg ctgatggtca
     2281 tagaaagaat cctggctccc ggtgcgttga atagtttgaa gatgggtggg cgtcccataa
     2341 tatatcctcc ctggatggta aggctggtgt tcccgatgat tgcccatctg aaaattttat
     2401 tgaaattggc ttcaatggct ccataactga aattaccacc tgctattccc ttcaatccgg
     2461 attttacact gacattgaag atgggataag atgggtttct tacaataaag taagtattga
     2521 aaaagttgat ctgtctgcct ttgatatatt gataggaggc tgatatttca ctatttatat
     2581 actcgccgga ctctccggga tttattcctt taaaatcgta ttcaaaatat ggatcaatgg
     2641 tttctcttgt ggtcgccagt tttatgctat gtgaagcact tgttttgaaa tagctgaatt
     2701 catgtttatc gatttcgaca aacaaattac tataatattg attaaaccat tgatttctga
     2761 atctgaacaa tctgttacct tcaacatctt tgttccaggt atatttgata ttactggttc
     2821 ttaatttgtc aaagtaccag gcaaccgatc caccgtattt cagggcattg tcgtgtgtac
     2881 catatgcaaa gtatgcttcc aaacccaggt ggggcataag tttcatgctt gaatggacgc
     2941 tgagtccgaa cctgttccct tcaaataaat ttttcctgta aatagataac atgtcgattg
     3001 ctaatgggcc tatcggtaac tgaccattca ggattagttg catattctgc aatatatatc
     3061 cggatggtcc gcttcgcaac aatacatctc caaatacata agctgtacta tcttgcctgt
     3121 tcaatggcat catccgcata ctgtcaatgc ttggcagggc tcctgtctgg tattctgttt
     3181 tattgatttt agagggttca atggggatgg gcagaaaggt gatcttatca aaaatattcg
     3241 tctgtttaac caaaaaggga tgttccttcc caaaggctga gggtaacttg acttcagtga
     3301 tgaatttata agggaaccat tggttgttga cacgccaata ctcctgatat aatacaaatg
     3361 gatacaaatc cataaaggtg ttcttcatgg tgattttttg aactgcccag tcattggccg
     3421 atatgatgat cattcctttg aagcaattat agcctgaatt ccttggccaa taatcaattt
     3481 tgtacaatgt gtcattggct tcctcataga ctttgtcgag gttaaaatga tactttaacc
//
LOCUS       DAOQQN010000342         3533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_13082_length_3533_cov_4.336975, whole genome shotgun sequence.
ACCESSION   DAOQQN010000342 DAOQQN010000000
VERSION     DAOQQN010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13082_length_3533_cov_4.336975"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="PLG24_11255"
     CDS             <1..1122
                     /locus_tag="PLG24_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HMZ25515.1"
                     /translation="VGGLISAFTEYYTGLGKKPVLGIVQKSSTGAATNIIAGLSTGMI
                     STFGPIILFAGAIWAAYSFAGFYGVALAASAMMATTAMQLAIDAFGPIADNAGGIAEM
                     SELPKEVRTKTDILDSVGNTTAAIGKGFAIASAALTALGLFAAYVTFTGIDGINIFKA
                     DVLAALFIGAMIPVVFSALAMSSVGKAAMDMVKEVRRQFKEIPGIMEGKATPEYGKCV
                     EISTKAALREMMLPGALTIVVPILVAIFMGAEALGSYMAGVTVSGVLWAIFQNNAGGA
                     WDNAKKSFEAGVEIDGVMTYKGSDAHKAAVTGDTVGDPFKDTSGPSMNILIKLTCLIG
                     LALAPILGGHNAKHDTGLKSTPKIEKMYKQEAPTNVIKF"
     gene            1411..3195
                     /gene="sppA"
                     /locus_tag="PLG24_11260"
     CDS             1411..3195
                     /gene="sppA"
                     /locus_tag="PLG24_11260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HMZ25516.1"
                     /translation="MKSFLKIVLGSCVGVIFAMALFSFLSVIAFSSMSSSLTGGGGKE
                     ISPNSVLRISLEQPMPDKTNNIESSPLSFANRTFVGIVDAVKLIKHAKTDENIKGIYI
                     NPGPGMAIGSAALKDLHDALLDFKTSKKFVIAYGEALSQGGYYLSSTADDIYLNPVGE
                     VYFKGLGMSIMYFKNLMDKLKIKMNVFYVGKFKSATEPFRSDHMSPENRLQLKTFLNG
                     MFSQMVEDIARNRKLDPAAVKKVADELSIQAPEDALALNFIDKLAYEDEVFEEIKTKL
                     KLKAKDKINFVSLNEYYDGTTLEKDYNAKEKIVLIYAEGEINDSDSHDAVIGGKSYVR
                     YLRKARLDDKVKAVVLRVDSPGGSAYASEQMWHEIDLLKKAGKKVIVSMGNLAASGGY
                     YISCAADRIFANNNTITGSIGVFGMIPNVSGFYNDKLYLTMDSVKTGPNGLGINGYYD
                     MTPAETSLIQRSIESIYKTFTKRVADGRKLSIETVDSLAQGRIYSGKDAVKIGLVDEI
                     GDVEDAIASAASMAKLKEYRLVEYPETKPPFEKFMEKLSGKETDDNAVKAAVTRELKA
                     IAPEYIEVKRLLQNNRIQARLPMTITIN"
     gene            3219..>3533
                     /locus_tag="PLG24_11265"
     CDS             3219..>3533
                     /locus_tag="PLG24_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HMZ25517.1"
                     /translation="MKIDIIAVIPDLMKSFFENSIIKNARTKGHLTINVIDLRNYGLG
                     NYKQIDDYQYGGQAGMVLMPEPLSNCIEMLQASTLYDEIIYLTPDGTTLTQKTSNKLS
                     LKE"
BASE COUNT         1109 a          682 c          770 g          972 t
ORIGIN      
        1 gtaggaggat taatatcagc atttacagag tattatacag gattaggcaa aaaaccggta
       61 ttgggcattg tccaaaaatc ttcaaccggt gccgcaacca atatcattgc ggggctttca
      121 acgggtatga tttctacttt tggcccaatc atattatttg ccggagcaat ctgggctgca
      181 tacagctttg ccggttttta tggtgtagct cttgctgcct ctgccatgat ggcaactact
      241 gccatgcagt tggctataga tgcatttggt ccgattgcgg ataatgcggg aggtattgct
      301 gaaatgagtg aactgccaaa agaagtaaga acaaaaacgg atattcttga ttccgttggt
      361 aatacaactg cagctatcgg aaaaggattt gctatcgcat cagccgcttt gactgcgctt
      421 ggcctttttg cagcctatgt caccttcacc ggtattgatg ggattaatat ctttaaggct
      481 gatgtgcttg ctgctctatt tatcggagcc atgatacctg tcgtgttctc agctcttgcc
      541 atgagctcgg taggtaaagc agccatggat atggtgaagg aggtaagaag acaattcaag
      601 gagatccccg gcataatgga aggaaaagcg acacctgaat acggcaaatg tgttgaaatc
      661 agtacaaaag cagctctcag ggaaatgatg ttgcccggtg cacttaccat cgttgtccca
      721 atactggtgg ccatctttat gggtgctgaa gccttgggtt cgtatatggc aggtgtgact
      781 gtttcaggtg ttctttgggc tatcttccaa aacaatgcag gaggggcatg ggacaatgcc
      841 aaaaaatcct tcgaagctgg agtagaaata gatggtgtaa tgacctacaa aggctcagat
      901 gcccataagg ctgctgtgac aggtgacaca gttggtgatc catttaaaga tacatcagga
      961 ccttcgatga atatcctgat caaattgact tgtctgattg gcctggcatt agcaccaata
     1021 ctgggcggac ataatgcaaa gcacgataca ggcctgaaat ctactccgaa gatcgagaag
     1081 atgtacaaac aagaggcccc aaccaacgtt ataaagtttt aacaaacaga taattctatt
     1141 gataaaaggc tgtaaaatac ttacagcctt tatttttttt atttggtctt gaaaaaagta
     1201 ccttatatcc aaaaatttaa actaataagt actttttggt gtatcattgt ataaagtgaa
     1261 ctaaaaatat gtattcaact ggtttttgat cattttaaga ttccaaactc aaatacagta
     1321 tgatacttaa cacatattat ttcaccactt tatacataaa acaacagccg taaaagaact
     1381 aaaaatatat aataattaag tttctctttc atgaagtcat tcttaaaaat cgtcttagga
     1441 tcctgcgtag gtgtcatttt tgccatggca ctattttctt ttctctctgt tattgctttt
     1501 tcttccatgt cttcgtcttt aaccggagga ggcggaaagg aaatttcccc caacagtgtt
     1561 ttgcgcatta gtttggagca acccatgcct gacaagacaa acaatatcga aagtagccca
     1621 ctttcctttg ccaatcgcac ttttgtaggt attgtcgatg ctgtaaagtt aatcaaacat
     1681 gccaagactg atgagaatat caaagggatc tacatcaatc ctggaccagg catggccatt
     1741 ggatctgcag cacttaaaga cctgcatgat gcgcttttgg attttaagac gagtaagaaa
     1801 ttcgttattg cctacggaga agcactttca caaggtggat actaccttag ttccacagcg
     1861 gatgacatct atcttaatcc tgttggtgaa gtatatttta aaggcttggg gatgagcata
     1921 atgtatttta aaaacctgat ggataagcta aagattaaga tgaatgtctt ttatgtcggc
     1981 aaattcaaat ccgccactga gccgttcaga tccgatcata tgagcccgga aaacagactt
     2041 cagttgaaga catttctcaa tggtatgttt agccaaatgg ttgaggatat agccaggaac
     2101 aggaaacttg acccggcagc agttaaaaaa gtagctgacg aattatcaat tcaggccccc
     2161 gaagatgctt tagctctgaa tttcattgac aaactggctt atgaagatga agttttcgaa
     2221 gaaataaaaa caaagctaaa attaaaagcc aaggataaaa tcaattttgt ttccttaaat
     2281 gaatattatg acgggacaac cttggaaaag gactataatg caaaagaaaa aatcgtcctg
     2341 atatatgcag aaggtgaaat aaatgattct gacagccatg atgctgtcat tggcggcaaa
     2401 tcatatgtgc gttatctcag aaaagcaaga ctggacgata aggtgaaggc agtagtccta
     2461 agagtggaca gcccgggggg tagtgcatat gcctcagagc aaatgtggca tgaaatcgac
     2521 ctgcttaaaa aagcaggtaa aaaagtaatc gtgtccatgg gcaatctggc agccagcggc
     2581 ggatattaca tttcatgcgc agcagacaga atatttgcca ataataatac aattaccggg
     2641 tcaatcggtg tttttggcat gataccaaat gtgtccggat tttataacga caagctatac
     2701 ctcactatgg attctgtgaa gaccggacca aatggattgg gtatcaatgg ttattatgac
     2761 atgacacctg cagaaacctc cttgattcaa cggtcgatcg aatcaatcta caaaaccttt
     2821 acaaagagag tagctgatgg aagaaaatta tcgattgaga cggttgacag tcttgctcag
     2881 ggccgaatct attcaggcaa ggatgctgtt aagattggcc tggtcgatga aataggtgac
     2941 gtggaggatg ctattgccag tgctgcctct atggccaagc taaaagaata tcgtctggtt
     3001 gaatatcctg agacaaagcc tcctttcgaa aaattcatgg aaaaactttc aggaaaggaa
     3061 actgacgaca atgcggttaa agctgcagtt acaagggaac ttaaagccat tgctcccgaa
     3121 tatattgagg ttaaacgctt gcttcaaaac aatcgcatac aggcccggct ccccatgaca
     3181 attactatca attgacaatc tgaagccctg tattgcctat gaagatcgat atcattgcag
     3241 tcattccgga cctgatgaag tccttttttg agaattccat catcaaaaat gccaggacaa
     3301 aaggccattt gacaatcaat gtcattgatt tacggaatta tggtctaggg aattacaagc
     3361 agattgatga ttatcagtat ggcggtcagg caggaatggt actgatgccc gaaccattga
     3421 gcaattgtat tgaaatgcta caagcctcaa ccctttatga tgaaataatc tatctgacac
     3481 cggatggaac tacactcact caaaagacat caaacaagct gtcactcaag gaa
//
LOCUS       DAOQQN010000343         3520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_13159_length_3520_cov_4.443290, whole genome shotgun sequence.
ACCESSION   DAOQQN010000343 DAOQQN010000000
VERSION     DAOQQN010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13159_length_3520_cov_4.443290"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLG24_11270"
     CDS             <1..222
                     /locus_tag="PLG24_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25518.1"
                     /translation="ITSDRHEMERELATFRNEYNLKPKWTQKGPYYYLKAGAYRTKLE
                     GYADMKAISTKYPGALFIVEKVKKQLILD"
     gene            254..1660
                     /gene="rodA"
                     /locus_tag="PLG24_11275"
     CDS             254..1660
                     /gene="rodA"
                     /locus_tag="PLG24_11275"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HMZ25519.1"
                     /translation="MNKRAVQNETGRVDWVLLSLYLALVAIGWTMIYAVSHDKNTSGS
                     ILSLDYNGGRQIIWIGISLFVLFFILVFDSKIWRTFSYGFYAIGIILLTLVLFFGKVI
                     NGQKAWFGIGMFTFQPAEIAKFGTALALANLLSDYKMKLSEPRAAMVAISLVLFPPLL
                     IMLQPDAGSALVFFSFFILLFREGLNASLYIIILSFAGLFILSLLFGFDNTYLVILIA
                     GLAIMVYHLPNRLYYMLAVGMLGIGGFIAVNSGNAPELLGVLTGILLVLAGILVWKKK
                     SQLVFVLVSVLTIASLTSFFSNFVFNILEPHQQERINVWLHPERCDPRGSLYNVLQSK
                     MAIGSGGLSGKGFLNGTLTKLNYVPEQTTDFIFCTVGEEHGFIGTLIVISLYLTLLLR
                     LSFVAERQRLNFSRNYIWGVAGILFFHFLINIGMTMGLVPIIGIPLPFVSYGGSSLIG
                     FTVLLGVVIKLDSIRNKD"
     gene            1800..2939
                     /gene="tgt"
                     /locus_tag="PLG24_11280"
     CDS             1800..2939
                     /gene="tgt"
                     /locus_tag="PLG24_11280"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HMZ25520.1"
                     /translation="MTNKFELIHTDDKSRARTGILSTAHGDILTPVFMPVGTVGTVKG
                     VHQSELSDKIKARIILGNTYHLYLRPGVETVAGAGGLHKFIGWDKPVLTDSGGYQVFS
                     LAHRRKIREEGVTFQSHIDGSRHLFTPESATEVQLQLGADIIMAFDECTPYPCEYKYA
                     ERSMHLTHSWLDRCLSYFDAIDEFPHGYRPTLAPIVQGGTYSDLRRQSAEYIASKSRH
                     VNAIGGLSVGEPAEEMYRITEEVTTILPKEAARYLMGVGTPANILTCIGLGIDLFDCV
                     LPSRNARHGLLYTWDGIINIKNEKWKMDYSPIDSTSRADTSRQHSRAYLRHLIHVGEL
                     LGAQIATIHNLAFFLELVDTARERILDGTFADWSMQVIPRLEHRL"
     gene            3056..>3520
                     /locus_tag="PLG24_11285"
     CDS             3056..>3520
                     /locus_tag="PLG24_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ25521.1"
                     /translation="MEVKKVVITGGSRGIGEAIALKLAGQGMHIAILAKTTEPQPKLK
                     GTIYSVAEKIELQGGKAYPHQLDIRDDAAISQAISTVAGQMGGIDILINNASAISLTN
                     TEDTSAKRFDLMFDINVRGTFLVTQACIPHLRKSSNPHILTLSPPLSVEPRWF"
BASE COUNT          977 a          793 c          798 g          952 t
ORIGIN      
        1 attacttccg acagacatga aatggaacgc gagttggcaa cattcaggaa tgaatataat
       61 cttaagccaa aatggactca aaaaggcccc tattattatc tgaaagcagg agcctaccgc
      121 accaaactcg aaggatatgc cgacatgaag gctatatcaa caaaataccc cggagcactt
      181 tttatcgtag aaaaagtcaa aaaacaactc atcctcgatt aattgactgc atcgaataac
      241 aaaaccaccg gcaatgaata aaagggctgt tcagaatgaa accggcagag tggactgggt
      301 tttgctctcc ctttatcttg cccttgttgc tattgggtgg accatgatat atgccgtcag
      361 tcatgacaag aacacttcag gaagtatact gagccttgat tataatggag gcaggcaaat
      421 tatctggata ggaatatcct tgtttgtcct gttttttatt ctggttttcg acagcaaaat
      481 ttggcgtacc ttctcttacg gtttctatgc catcggcatc atactattga cattagttct
      541 attcttcgga aaggtcatca acgggcaaaa agcgtggttc ggtataggca tgtttacctt
      601 tcagccagca gagattgcca agtttggaac tgccttagcc ctggccaatc tgctcagtga
      661 ttataaaatg aagctttctg agccaagggc tgccatggtg gcgatttcct tagttttatt
      721 cccgccgttg ctcattatgc tccagcccga tgcaggctct gccctagtat tcttttcctt
      781 cttcatcctc ttattcaggg aggggctcaa tgcttccctg tatataatta tcctctcctt
      841 tgcagggttg tttatcttat ccttgttgtt cggatttgac aatacctatt tagttatttt
      901 gattgcaggt ctggccatca tggtatacca cctgcccaac aggctctatt atatgttggc
      961 ggtaggcatg ttgggaatcg gaggcttcat tgcggtcaac agtggtaatg cacctgagct
     1021 tttgggtgta ctgaccggca tccttttggt cctggcaggc atcctcgtat ggaaaaaaaa
     1081 gtcacagttg gtctttgtat tggtgtctgt acttaccatt gcatccctga catctttctt
     1141 ttctaatttt gttttcaata tcctggagcc ccatcaacag gaacggatca atgtatggct
     1201 ccatccggaa agatgcgacc ctcgcggctc cctatacaac gtgctccaat caaagatggc
     1261 aatcggctca ggggggcttt ccggaaaagg tttcctgaat ggtacactga ccaagctaaa
     1321 ctatgtcccg gagcagacga cggactttat attttgtacg gtcggagaag aacatggatt
     1381 catagggacg ctgatcgtca taagtcttta cctgaccctg ttgttaaggc tgtcgttcgt
     1441 ggctgaacga caaaggctca atttttcgag aaattatatc tggggagtgg ccggcatcct
     1501 gtttttccac ttcctgatca atataggtat gacaatgggt ctggtcccaa tcataggtat
     1561 accgttgcca tttgtcagtt atggcgggtc atccttaatt ggattcacag tcctgctggg
     1621 agtggtcatc aagcttgaca gtatcaggaa taaggattaa taaaaacagg taagtcaggc
     1681 cggataaata catcaactca tacgtataat tgattagttt aaaatttttt aaaacctgaa
     1741 caagacctgc tagataattt gtaaaataaa atgcggttat ttatctctat tattaaataa
     1801 tgacaaataa gtttgaatta atccatacag atgataaaag ccgggcacgg accgggatat
     1861 tatctacggc acatggtgac atactaacac cggtattcat gccggttgga acagtcggta
     1921 cggtcaaggg tgtccatcag tcggaattgt ccgataaaat aaaggcacgg atcatcctgg
     1981 gcaacaccta tcacctgtat cttcgaccgg gtgtcgaaac agtagcaggt gcaggaggac
     2041 tccacaaatt tatcgggtgg gacaagcctg tcctgacgga cagtggaggt tatcaggttt
     2101 tttccctggc ccacagaaga aaaatcaggg aggaaggagt gacctttcag tctcacatag
     2161 atggaagcag acaccttttt acccctgaat ccgcaaccga agtgcaatta cagctgggtg
     2221 cagatatcat aatggctttc gatgagtgta caccctaccc ttgcgaatat aaatatgccg
     2281 aaagatccat gcatctgaca cattcctggc tggacaggtg cctgtcgtat tttgatgcca
     2341 tcgatgaatt tccacatggg taccggccca ctctggctcc aatcgttcag ggaggtacct
     2401 acagtgactt aagacgtcaa tcggctgaat atattgcttc caaatccaga catgtcaatg
     2461 caataggcgg cctctccgta ggggaaccgg cagaagaaat gtaccgtatt accgaagaag
     2521 ttacaactat attgccgaaa gaggcggcga gatacctgat gggtgtaggc actccggcaa
     2581 atatactcac ctgtatcggc ctcggaatag accttttcga ctgcgtcctc ccatcgcgca
     2641 atgctcgcca tgggttactc tatacctggg acggcatcat taatatcaag aatgaaaaat
     2701 ggaaaatgga ttattcgccg atcgacagta cttctcgggc cgatacctcc agacagcatt
     2761 cacgggctta tctcagacat ctgattcatg taggtgaatt gcttggcgca cagattgcaa
     2821 ccatccacaa cttagcattc tttctggaat tggtggatac ggcccgggaa agaatattgg
     2881 acggcacatt tgcagactgg agtatgcagg taataccacg acttgagcat agattgtaag
     2941 aaaggtaagc tatgcaagta cggatatttt gctatcttat ttgagatttt cacaccgaga
     3001 agaaaaatat cccgttcaaa ccagaataaa atggcctttt tgcataactt taggcatgga
     3061 agtaaaaaaa gtagttatta ccggtgggtc ccggggaatc ggtgaagcca tagccttgaa
     3121 gcttgcagga cagggaatgc atattgccat actcgctaag acaacagagc cacagcccaa
     3181 actaaaagga actatctaca gcgtcgctga aaagattgaa ttgcagggcg gcaaggccta
     3241 tccccaccaa ttggatatca gggatgatgc tgccataagt caagcaattt ctactgtagc
     3301 cggacaaatg ggtggaatcg acatcctgat caacaacgcc tcggctattt ccctcacaaa
     3361 tacggaggat acatctgcca aacgcttcga ccttatgttc gatatcaatg tcagaggaac
     3421 cttcctggta acacaggcct gtatcccgca cctcagaaag agcagtaatc ctcatattct
     3481 gactctgtct ccacccctca gtgtcgaacc acggtggttt
//
LOCUS       DAOQQN010000344         3510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_13218_length_3510_cov_4.371346, whole genome shotgun sequence.
ACCESSION   DAOQQN010000344 DAOQQN010000000
VERSION     DAOQQN010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13218_length_3510_cov_4.371346"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1650
                     /locus_tag="PLG24_11290"
     CDS             <1..1650
                     /locus_tag="PLG24_11290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ25522.1"
                     /translation="NEATARDVWFKFTATANFIHLTVNGKSLGNPGGTLVSPQVILFS
                     GPCNALVEQDCFSDAFGINSLEVFEGPLVPGQTYYINISARDNHLGTFQLCLNNYNQN
                     FVPDGDCPTSRVLCDKSSVFIEAVFNPGNITDEIPLFSCVREEIASSWFRWTCSKSGT
                     LTIKLTPTKPEDDLDFALFELPNGIDDCNGKKQLRCGAAGENVGEPYNNWKQCTGPTG
                     LNLTSTDINEDPGCNNGNDSWVKYIDMVEGVSYAFFVNNFSQSGQGYTMEFGGTGEFL
                     GARASFDVTPDGTCYEDTLHFINTSIAPTDPIVSYQWEFGANSNIGSSTQQNPPGVIY
                     DGPGYKSVSLTITSQDGCVHTFTKNFIAKCCIDPLKVSMAVQPYNEVKLGDPIVLTAT
                     TENAVGSTIFEWFPSDLITNCFNCREVTLVPVGNYIFTVTAQDQKGCISTDSLEIRVV
                     DSKDVVAPNVFSPNYDGINDYFTVFPNRGAKFIKRLLVFNRWGACVYEGKELTPGDDS
                     IGWDGTFKGKECTPDVYAYFAEVEFLNGRIQIIKGDVTLVR"
     gene            complement(1695..2423)
                     /locus_tag="PLG24_11295"
     CDS             complement(1695..2423)
                     /locus_tag="PLG24_11295"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HMZ25523.1"
                     /translation="MKHFGLIGKSLKHSFSKKYFDEKFKAFGLLDFDFRLIEIPDIED
                     LPEKVKEYELSGFSVTIPYKEAIIPFCHSLSPEAEKICAVNCVKVTDNGWVGYNTDYV
                     GFRDTLPLDDLAKGGTAIVFGTGGASRAIIHVLSRLGLEIINVSTSSKKGTIHYQALK
                     DQIIKKALILVNCTPLGTFPEVDKNVPINFSAIHKGQICYDLVYNPDQTKFLRLSAQQ
                     GARTINGMKMLIIQAEEAWKIWNN"
     gene            2797..3183
                     /locus_tag="PLG24_11300"
                     /pseudo
     CDS             2797..3183
                     /locus_tag="PLG24_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
     gene            3190..>3510
                     /locus_tag="PLG24_11305"
     CDS             3190..>3510
                     /locus_tag="PLG24_11305"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HMZ25524.1"
                     /translation="MQQFLPELLSGSKYSSRLPIGKDEILKTFKYEELRRFYRDWYRP
                     DLMAVAVVGDITKEKAEQMIKNHFSAIKGPKNPRTREYAEINPFKEAKAMVVTDKEAT
                     SYQFI"
BASE COUNT         1024 a          655 c          751 g         1080 t
ORIGIN      
        1 aatgaagcta ctgcaaggga tgtttggttt aaatttacag caacagcaaa ttttatccac
       61 ctaaccgtta atggtaaatc ccttggaaat cccggtggga ctttagtttc accgcaggtg
      121 atcctatttt ccggtccttg caacgcattg gtagaacagg attgtttcag cgatgcattt
      181 gggatcaatt cacttgaagt ctttgaagga cctttggttc cgggtcaaac ctattatatc
      241 aacatcagtg cccgggataa tcatttgggg acttttcaat tatgtttgaa caattataat
      301 caaaattttg tcccagatgg cgattgcccg acaagtagag ttctttgcga taaatcttcc
      361 gtattcattg aagctgtgtt taatcccgga aatataaccg atgaaattcc gcttttttct
      421 tgtgtaaggg aggaaattgc ttcttcctgg ttcagatgga cttgtagtaa gtctggtaca
      481 ctgaccatca aactcacacc gactaaacct gaagacgatc ttgattttgc cttgtttgaa
      541 cttcccaacg gcatagatga ctgtaatggt aaaaagcagc tgcgttgcgg ggcggcagga
      601 gaaaatgtcg gagagcctta taataattgg aaacaatgta ccggacctac cggattaaat
      661 ttgaccagta ccgatatcaa tgaggatccg ggatgtaaca atggaaatga tagttgggtg
      721 aaatacatcg atatggttga gggcgttagt tatgcctttt ttgtcaataa tttttcacag
      781 tcaggacagg gttatacgat ggagtttggt ggcacgggag agtttctggg agcgagggct
      841 tcctttgatg taactcctga tggtacatgc tatgaagata ctctgcattt tatcaacacc
      901 tccattgctc cgacggaccc aattgtttct tatcaatggg aatttggggc caattccaat
      961 attggaagtt cgacacagca aaatcctccg ggtgtcattt acgatggtcc gggctataag
     1021 tctgtttcat tgactatcac ttcacaagat ggatgtgtac atacctttac caaaaatttt
     1081 attgctaagt gttgcattga tccactgaaa gtctctatgg ctgtacaacc atacaatgaa
     1141 gttaagttgg gtgatccgat tgtattgact gcaacaactg agaatgctgt tggatcgaca
     1201 atatttgaat ggtttccatc cgatctcatt accaattgtt ttaattgtag ggaggtaacc
     1261 cttgttccgg tagggaatta tatctttacg gttactgcac aagatcagaa aggttgcatt
     1321 tctactgact ctttggagat acgggttgtg gatagtaagg atgttgtggc gcccaatgta
     1381 tttagcccta attatgatgg aatcaatgac tactttacag tattccccaa tcggggggct
     1441 aagtttatca aacgcttgct tgtattcaac agatggggag catgtgtata tgaaggaaag
     1501 gaattgacac cgggggatga ttcaataggt tgggatggta cttttaaagg aaaagaatgt
     1561 accccggatg tttatgcata ttttgccgag gttgaattcc tgaatggacg tattcagatc
     1621 atcaagggtg atgttacgtt ggttcgttaa atctgcttag gtaaatagtt gaaatcacta
     1681 tagtattatg attttcagtt attccagatc ttccatgctt cttcagcctg aataatcaac
     1741 attttcatgc cattaattgt cctggctcct tgctgtgccg atagtctgag aaactttgtc
     1801 tggtcgggat tgtacaccag atcataacag atctgacctt tatgtattgc agaaaaattg
     1861 attggaacat ttttgtctac ttcgggaaag gttcccaggg gagtacaatt gaccaggata
     1921 agagcctttt tgatgatctg atcctttagg gcctggtagt gaatggtccc tttcttcgat
     1981 gaggtggaga cgttgataat ctccagtccc aatcgtgaaa gtacatgtat gattgctctg
     2041 gatgcacctc ctgtcccaaa aactatagca gtgcctccct ttgccaggtc atctaaagga
     2101 agggtgtccc tgaagcccac ataatctgta ttataaccaa cccatccatt gtcagttact
     2161 ttcacacagt tgactgcaca tatcttttca gcttcaggac ttaaggagtg acaaaagggt
     2221 attatagctt ctttataggg tatcgttacg gaaaatccgg atagttcata ctccttgacc
     2281 ttttcaggta aatcttcgat atccggaatt tcaatcagtc taaaatcaaa atcaagcaaa
     2341 ccgaatgcct tgaatttctc atcaaaatac tttttcgaga aggaatgttt taggctcttg
     2401 ccaatcagtc caaaatgttt catccaacaa ttaaaaaaat gaaatgtaaa attatatcaa
     2461 tcagggatat attacaatta ttctttcatt cctcctgccc attccgttag aaagtatata
     2521 tttgcatacc agtcatcata agaattgacc tttcacctta atgaatactt tataatcttt
     2581 gtggctggat ttttacgcaa ctttttaata attctcttca tactaaacac ggttttcaat
     2641 atgaaaaaac aatttgtaaa gttcttcctt cctgttatgg tattattcat gttgggctca
     2701 atatcccgga ttgatgccca ggatctctcc aaaccgattc ccattgataa gaacttgacg
     2761 attgggaagc tatctaatgg acttacttac tacattatgc caaactcaaa acctgaaaat
     2821 aaggtagaac tccgattggt catcaatgcc ggatcgatac ttgaagatga tagtcagcaa
     2881 ggtttggcac actttatgga acacatgaat tttaatggaa ctaagaattt tgaaaagaac
     2941 caataggtca gtttcctgca atccatcgga gtgcaatttg gagccgatct gaatgcatat
     3001 accggttttg accagacagt ttacattctg cctatcccga ccgataagcc cggtaatttg
     3061 gagaaaggct tccagatttt ggaagattgg gctcataatg ccttattgtt ggataaggat
     3121 attgacgatg aaagaggggt agtgcttgag gagagtcggg caagaggaaa aaacgccaat
     3181 gaacgtatga tgcagcagtt tttacctgaa ttattatcgg gctctaagta ttcatccaga
     3241 ttacccatag gcaaggacga aatccttaaa actttcaaat atgaggaatt aaggagattt
     3301 tatcgtgatt ggtaccgccc ggatttaatg gcagtggctg tggtgggtga cataacaaag
     3361 gagaaagctg aacagatgat caaaaatcat ttttcagcca tcaaagggcc taaaaatcct
     3421 cgtacgagag aatatgctga aataaatccg tttaaggagg ccaaagcaat ggttgttact
     3481 gataaggaag ctaccagtta tcaatttata
//
LOCUS       DAOQQN010000345         3492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_13338_length_3492_cov_3.649113, whole genome shotgun sequence.
ACCESSION   DAOQQN010000345 DAOQQN010000000
VERSION     DAOQQN010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13338_length_3492_cov_3.649113"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PLG24_11310"
     CDS             complement(<1..715)
                     /locus_tag="PLG24_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25525.1"
                     /translation="MKKNVQYNFLEALVFFLLFISFKQGITQTTIFRERFNIAPNTEA
                     NGATSGLSEEGIAWTATCPLCFINNPPSPAGRHFDVQNGEFENKANEGVGQWITTGAI
                     DISTATKLDFTVDYHSNLPWPSGGNMESNNECGGCAGDPDNYPNTGPCGSCWDFINVQ
                     LILDGTTVYNETVGNGNNVQTYQFSFSSACFPPGTYSNAVIRIRTQTWSLGDPEIIWF
                     DDVHLVAYQVPRHEKRLRRI"
     gene            1147..1764
                     /locus_tag="PLG24_11315"
     CDS             1147..1764
                     /locus_tag="PLG24_11315"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMZ25526.1"
                     /translation="MSVTNAFVIVSGLLSVLAFRNPALLYKLIGWPHRSSTEREYYRL
                     FTGGLVHGDYIHLLVNLFVMYQFGAIVEMEYKVLFGENGVYYFLALIVLGVIVPNLFD
                     LMLHKNQPNYRSLGASGSVSAVLFAYVLFNPWSKLYLYGILPLYSIVAAVLYIVYSVY
                     QDKKGKDNINHMAHLIGAVFGFVFTLALKPSLGPFFVQQLLSFSF"
     gene            complement(1754..>3492)
                     /locus_tag="PLG24_11320"
     CDS             complement(1754..>3492)
                     /locus_tag="PLG24_11320"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HMZ25527.1"
                     /translation="GSGGHIDMYLIFGESPLEVVTNYTKLTGTPHMPPLWALGFHQSK
                     WSYVPEQALMDVAAKFRELNIPCDSIYLDIDYMRGYRVFTWDKRRFPDPKAMLKKLRH
                     QGFQIVAILDPGIKIDKRYAIYKEAISEDFFIKNPDGSTTSGPVWPGYCHFPDFTSRK
                     VRRWWADHVAELVRVGLAGIWNDMNEPALFNKADMIQTQRTLNEDVRMNFEGESGDFA
                     EGHNLYGMLMSEATMEGFRQAGDRRPFVLTRSTYAGGQKFAAVWTGDNVATWEHLRIA
                     NYQCQNLALSGFSFAGSDIGGFVGNPDGELFCRWLQLALFHPFFRNHSSKDFAEQEPW
                     SFGEKWTDCARQIIHWRYRLLGYIYTVFRRYVHEGIPMIKPVNFVHAKEKRPPCFDQF
                     YLGDALLAIPVMEPGQSGVRISLPEGYYYDVNSARQFIGNNDYFIRVKMDAAPALLKG
                     GHILPIWPAMQYVNEKPLEEMEIWCGFSDAEKVISRMYLDEFDGHSYLEGNYQETVFE
                     YNNCQTYVLININQSGYYQAAIRKYTFKFPGFKNKIKSIESDGNKLEISKDDPIIVLE
                     KMPEKIRIDLKS"
BASE COUNT          943 a          761 c          642 g         1146 t
ORIGIN      
        1 gaatacgcct cagacgtttt tcatgcctgg ggacttggta agcgacaaga tgcacatcat
       61 cgaaccagat gatctcaggg tctcctaatg accaggtttg ggttcttatt cttattactg
      121 cattggaata tgttcccggt ggaaagcaag ctgaagaaaa actaaattgg taagtctgaa
      181 cattattccc attgccgacg gtttcattat atacagtagt tccgtccaag atcaactgga
      241 cattgataaa atcccaacaa ctaccacatg gtcctgtatt gggataatta tccggatctc
      301 cggcacaacc tccacattca ttattagatt ccatattacc gccggaaggc cagggtagat
      361 tactatggta atcaacggta aaatcaagct tcgtagctgt cgaaatatca atggcacccg
      421 ttgtaatcca ttgtcccacc ccttcattgg ccttgttttc aaattcgcca ttttggacat
      481 caaaatgcct tcctgccgga gatggtgggt tattgatgaa gcaaagtgga caggttgctg
      541 tccatgcaat tccttcttca ctcaatcctg atgtagctcc atttgcttcc gtgttgggtg
      601 caatattgaa cctctccctg aatatcgtag tttgagtaat tccttgctta aatgagatga
      661 ataataaaaa aaagactaat gcctctaaga aattgtattg tacattcttt ttcaaaatat
      721 tccgacctta aattttccgg ttgattaact atatactttg acaatccttg acaaggaaat
      781 tgtgcacaaa gttaatcttt tatttatttc cctttaagaa tcatctatat tttttagtac
      841 tgttttcatt ggctatgggt tcttcatgtt gtacattact ttttgaaatt caagtaattg
      901 gacatgcttc aaatctatat gaatattaag gttttataga aattatatga atacgttgat
      961 ggaataaata ttcatttttt atgtaaggaa taaggttcct tgtacttttt atcaaagctt
     1021 tttatccatg cgggaaatgg atataaatta cttttattag ttcatttagt cgatgaaatt
     1081 ttcccatgtc tgaccagatc atagtgttga aggattatct tcgcattcaa aataacttca
     1141 acttttatgt cagttaccaa tgcatttgtg attgtttccg gcctactttc ggttttggca
     1201 ttcaggaatc cggctctttt gtacaaatta attggatggc cacatcgttc ctcaactgaa
     1261 cgggaatact acaggttgtt caccggagga ttggtccacg gagattatat acatcttctg
     1321 gtcaaccttt ttgtgatgta ccaatttggt gcgattgttg aaatggagta taaggtactg
     1381 ttcggagaaa atggtgtgta ttattttctg gctcttattg tactgggtgt cattgttcca
     1441 aatttattcg atctaatgct acacaaaaac caacccaatt acagaagcct tggagcatcg
     1501 ggttcagtta gtgcagttct gtttgcttat gttcttttca atccatggag taagctatac
     1561 ctttatggca tcttgccttt gtactctatt gtggcagctg tcctatacat tgtatatagt
     1621 gtataccagg acaaaaaagg taaggataac atcaaccata tggcccacct aatcggagcc
     1681 gtttttggtt ttgttttcac cttggctttg aaaccttctc tcggtccttt ttttgttcaa
     1741 cagttgcttt catttagctt ttgaggtcga tccttatctt ttcaggcatt ttctccaaaa
     1801 caattatcgg gtcatcttta cttatttcca acttgttgcc atcgctttcg atggatttaa
     1861 ttttattttt gaatcccgga aatttaaatg tatatttcct gatagcggct tggtaataac
     1921 ctgattgatt gatatttatt aagacatatg tttggcaatt attatattca aacacagttt
     1981 cctgataatt gccttccaga taggaatgac cgtcaaactc atcaagatac atacggctaa
     2041 tgactttttc cgcatctgag aatccgcacc atatttccat ttcttccagt ggtttttcgt
     2101 ttacatactg cattgcaggc cagatgggta atatgtgtcc gcctttgagt agggccggtg
     2161 ccgcatccat ttttacccga ataaagtaat cattatttcc tataaattgc ctggccgaat
     2221 tgacgtcata atagtatcct tcaggtaggg atatcctcac tccgctttga ccgggttcca
     2281 ttaccggtat ggccagtaat gcatcgccca gatagaattg atcaaaacag ggaggtcttt
     2341 tttctttggc atgaacaaaa ttcaccggtt tgatcattgg aatgccctca tgcacatatc
     2401 tccgaaaaac cgtatatatg tatcccaata atctataccg ccaatggatg atttgacggg
     2461 cacagtctgt ccatttttct ccgaaagacc aaggctcctg ttccgcaaag tcttttgacg
     2521 aatggtttct gaaaaatgga tggaaaagtg ctagttgtag ccaccgacaa aacaattctc
     2581 catcaggatt gcccacaaag ccaccaatat cgctcccggc gaacgaaaat ccacttaatg
     2641 caagattctg acattgataa tttgcgattc ttagatgctc ccaggtggct acattatcac
     2701 ccgtccacac tgccgcaaat ttttgccctc cggcataagt tgatctggtt aatacgaaag
     2761 gcctcctatc tccggcttgc cgaaaacctt ccattgtagc ttccgacatc aacatgccat
     2821 atagattatg tccctcggca aaatcaccgc tttccccttc gaagttcatc cttacatctt
     2881 cattcaaagt gcgttgtgtt tgaatcatat cagccttgtt gaacaaagca ggttcgttca
     2941 tgtcattcca tatacccgcc agaccaaccc ggaccaattc agctacatga tctgcccacc
     3001 atcttcttac tttgcgggac gtaaaatccg ggaagtgaca atatcccggc caaacaggac
     3061 cagatgtggt cgaaccatcc gggtttttta tgaaaaaatc ctcactgatg gcctccttat
     3121 agatagcata ccgtttatca atttttatac ccggatcaag aatggcaaca atctgaaagc
     3181 cctggtgtct caacttcttc aacattgcct tcggatcagg aaatctcctt ttatcccagg
     3241 taaatactct gtatcctctc atatagtcga tatccagata aatgctgtcg cagggaatat
     3301 ttagctctct gaactttgct gctacgtcca tcaaggcttg ttccggtaca taggaccatt
     3361 tactttgatg aaaacccaaa gcccataatg gcggcatatg tggcgtgcct gtcagctttg
     3421 tgtaattagt cactacttcc aatggcgatt ccccgaaaat caagtacata tcgatatgcc
     3481 ctccacttcc tg
//
LOCUS       DAOQQN010000346         3478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_13425_length_3478_cov_5.094654, whole genome shotgun sequence.
ACCESSION   DAOQQN010000346 DAOQQN010000000
VERSION     DAOQQN010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13425_length_3478_cov_5.094654"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PLG24_11325"
     CDS             <1..775
                     /locus_tag="PLG24_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HMZ25528.1"
                     /translation="LPDDRAKPYKTIEIIERLVDDSELIQYKENYGKTILCAYARIDG
                     WAVGIVANNREVIKTKKGEMQFGGVIYSDSADKAARFIMNCNQKKIPLVFLHDVTGFM
                     VGTRSEHGGIIKDGAKMVNAVSNSTVPKFSVVIGNSYGAGNYAMCGKAYDPRLIVAWP
                     TAKIAVMGGAQAAKVLLQIEMSALKGKGENIDPQREQEYFEKIKDKYDNQTTPYYAAA
                     RLWVDAIINPVDTRTWISMGIEAANHAPMEPFNPGVIQV"
     gene            775..1929
                     /locus_tag="PLG24_11330"
     CDS             775..1929
                     /locus_tag="PLG24_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HMZ25529.1"
                     /translation="MELTITTFKGLERVLAEELQELGAQDIVIGTRAVTCYGDKELLY
                     RANYELRTALRVLVPIHHFVANNTDSLYRKVKAFNFSGYFRPEQTFSIDAVVHSEYFN
                     HSQFVIHKVKDAIVDRMRADYGVRPSINVSTPDFRLHVRITGDRVTLSSDSSGDSLHK
                     RGYRTKTVSAPMSEVLAAGLVRLSAWDKKSIFIDPMAGSGTIAIEAAMAAAGIPAQYC
                     RKDFTFRKWKNFDNRLLTKIINSANERIITDLPPIFASDKSRLAVDNIKINLESLPLQ
                     NRVIASQFDFFNVRQENATIIMNPPYDERMKVSDVIRFYEEIGNHLKHKCSGSKAWVF
                     SSNIEAMKYIGLKPSKKFVLYNGPLECRFYGFDLYSGKRGDYLEHDAKAK"
     gene            2143..2874
                     /locus_tag="PLG24_11335"
     CDS             2143..2874
                     /locus_tag="PLG24_11335"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HMZ25530.1"
                     /translation="MKKYSVIIVAFFLLMWTGLQAQPYLGFKFGYNAGNTHAAKAFTN
                     ILPLKTLHGLTAGGVGGYNFNPYFALQAELNVTQKGFRITESKGFEIYDFPIDVGVDY
                     WNRFTYLELPILAKGKIGNDNVKAYGAVGPQFGYLMKGRAKANVNTLLPITVFDRSLN
                     LDNLGFKRFEVAGVAAAGVEFTLGPNANLFIEGRYTHGFTDYYKIAPIGTLQIDSDIQ
                     NRNLSGSIGITFNIGQAGTSSGNRF"
     gene            complement(2946..>3478)
                     /locus_tag="PLG24_11340"
     CDS             complement(2946..>3478)
                     /locus_tag="PLG24_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HMZ25531.1"
                     /translation="SFQLACIYFKFGELRLRNKKSFGTYPCYLTGNGLYEDNFKGLPN
                     PMYIADFRSFEVIKDDYEVKSNTFPKVLAIEQINPIEPRQRAIMAVRFSPEMIGTQFH
                     PEAYPEGMYDYFNQPDRRDSVIAEHGEERMNQMIRDLGHPNKITLTNSTILPNFLRQS
                     TKCVKECRLKEAAGAV"
BASE COUNT         1048 a          649 c          772 g         1009 t
ORIGIN      
        1 tcttccggac gatcgggcaa aaccatacaa gactattgaa atcattgagc ggttagttga
       61 cgattctgaa ttgatccagt ataaggaaaa ttatggaaag actatccttt gtgcctatgc
      121 caggattgat ggctgggctg ttggcattgt agccaataat cgagaggtaa ttaagactaa
      181 aaaaggagaa atgcagtttg ggggcgtcat atacagtgat tcagcagata aggcggcaag
      241 atttatcatg aattgtaacc aaaagaaaat ccctttggtg tttttacatg atgtcaccgg
      301 cttcatggtg ggcacacgat cagaacatgg aggtatcatc aaagacgggg ccaaaatggt
      361 taatgcggtt tcaaactcaa ctgtaccaaa gttttccgtt gtaatcggca acagttacgg
      421 tgccggaaat tatgccatgt gtggcaaggc ttatgaccca aggcttattg ttgcctggcc
      481 gaccgccaag atagctgtaa tgggtggcgc acaggcggca aaagtgcttt tacagattga
      541 aatgtcagca ttgaaaggta aaggagaaaa catcgatcct cagagggaac aggaatattt
      601 cgaaaaaatt aaggacaaat acgataatca gactacaccc tattatgctg cagcaagact
      661 ttgggtcgat gccatcatca atcctgttga taccaggaca tggatcagca tgggtattga
      721 agctgccaat catgcaccaa tggaaccttt caatccggga gtaatacaag tctgatggaa
      781 cttaccatta ctacatttaa agggctggag cgcgtattag ctgaggaatt gcaagagctc
      841 ggagctcagg atattgttat tgggacgcgt gccgtcacct gctatggcga taaggaattg
      901 ctgtacaggg caaattatga attgcgcact gcactccgtg tccttgtacc cattcatcac
      961 ttcgttgcca ataatacaga cagtttgtac agaaaggtaa aggcttttaa tttttccggt
     1021 tatttcaggc ctgaacagac attctccatt gatgctgttg tgcattctga atatttcaac
     1081 cacagccagt ttgttattca caaagtcaag gatgcaatcg tagacagaat gcgggcggat
     1141 tatggtgtca gaccttctat caatgtgtca acacctgatt tcagacttca tgtaaggata
     1201 acaggcgatc gggtgacctt gtcctctgac agttccggtg attcactcca caaaagaggc
     1261 tacagaacca agacagttag tgcaccgatg agcgaggttc tggcggccgg attggttcga
     1321 ttatctgcct gggataagaa atcaatcttt attgacccga tggcaggttc cggtaccatc
     1381 gcaatcgaag ccgccatggc agctgcgggc ataccggcac aatattgcag gaaagacttt
     1441 accttccgaa aatggaagaa ctttgacaac aggctgttga ctaaaataat taacagtgcg
     1501 aacgaacgaa tcattactga tttaccgcct attttcgcct ctgataagag caggcttgct
     1561 gtggataaca ttaaaatcaa cctcgagagt ctacctttgc aaaatcgggt catcgccagt
     1621 cagtttgatt tttttaatgt aaggcaagaa aatgctacga tcatcatgaa tccaccctat
     1681 gatgagcgca tgaaggtttc tgatgttata cgcttctatg aggaaatagg caatcatctg
     1741 aaacacaaat gttcgggtag taaggcctgg gtattttctt cgaatattga ggcaatgaag
     1801 tacattggtc taaagccttc taaaaagttt gtcctttata atggtccctt ggaatgtaga
     1861 ttctatggtt ttgatcttta tagtgggaaa agaggagatt acttagaaca tgatgccaag
     1921 gcaaaataaa ttgcaagtta tttggtaacg gatagaatgc atgattacat caacatattc
     1981 tattgttgaa aatcattata tggggagagc aatttccttt atgtatttaa agaaatatat
     2041 ttctttaaat aatattaata cattgaaaag atttttttaa ctatattttt ttaataattt
     2101 acgtcttata ggtaaattgt ttgatcataa aataaaaatg aaatgaaaaa gtattcagta
     2161 attattgttg cattcttttt gctaatgtgg acggggcttc aagctcaacc ttatctgggt
     2221 tttaaatttg gttacaatgc cggtaatacc cacgctgcaa aagcatttac caatatcctg
     2281 ccattgaaaa cacttcatgg cctgacggca ggtggagttg gtgggtataa ttttaatccc
     2341 tattttgcct tgcaggcaga attgaatgtt acccaaaaag ggttcagaat tactgaatcc
     2401 aaaggattcg agatttatga ttttccgata gacgtcggtg tggattactg gaacaggttt
     2461 acttacctgg agcttccaat cctcgctaaa ggaaagattg gcaatgacaa cgtcaaagca
     2521 tatggtgcag taggacctca atttggttat ttgatgaaag gacgagctaa agccaacgtt
     2581 aatacattgc ttccaataac tgttttcgat cgatccctga atctggataa tctcggtttt
     2641 aagagatttg aagtcgcggg agttgctgca gccggtgttg aatttacact tgggcccaat
     2701 gctaatctat tcattgaagg tcgttatacg cacggcttca cagattatta caaaatagca
     2761 ccaatcggga cattacagat tgattcagat attcaaaaca ggaatttatc cggcagtata
     2821 ggtattacct tcaatatcgg ccaggctggt acatcctctg gaaacagatt ttgaaatata
     2881 aaccatggaa taggagctca tcgaaatttc gatgggcttt tttattttat accgacaacg
     2941 catgattaga cagcaccggc agcttctttg agccgacact ctttgacaca tttggtactt
     3001 tgtcggagga aattagggag tattgtacta ttggtaagtg taatcttatt ggggtgtccc
     3061 aggtcacgaa tcatctggtt catacgctct tcaccatgtt cagcaatgac agaatctctc
     3121 cgatcaggct gattgaaata atcgtacata ccttcaggat atgcttccgg atgaaactga
     3181 gtgccgatca tttcagggga gaatctgact gccatgatag ccctttgtct aggctcaata
     3241 ggattgattt gttcgatggc cagaaccttt ggaaaggtat tggatttaac ttcataatcg
     3301 tctttaatta cttcaaaaga tctgaaatca gcgatataca taggattggg taatcctttg
     3361 aaattatctt catacagtcc gttaccggtc aagtaacaag gataggtgcc aaaagatttt
     3421 ttattgcgca atctaagctc tccgaatttg aaatagatgc aagccaactg gaatgaat
//
LOCUS       DAOQQN010000347         3475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_13440_length_3475_cov_6.185380, whole genome shotgun sequence.
ACCESSION   DAOQQN010000347 DAOQQN010000000
VERSION     DAOQQN010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13440_length_3475_cov_6.185380"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /gene="ricT"
                     /locus_tag="PLG24_11345"
     CDS             <1..980
                     /gene="ricT"
                     /locus_tag="PLG24_11345"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HMZ25532.1"
                     /translation="RADLKKATFYYTADGRVDFRELIRHFAKEFRVKIEMRQIGSRQE
                     SSRIGGMGSCGRELCCSTWLTDFKSVTTNAARYQNLAINQAKLSGQCGRLKCCLNYEL
                     DVYMEALEDFPTNADVLRYQGGQARLIKTDIFKKLMFYIKKDEKGREAFITLRVDEVL
                     RIQEDIAHGKLPEDITSTKVEAFEDDAVIKSEDLTGHIELPEEKRRKKKKKKNPENKP
                     MRHEGQKQEDKKETGNEGKDNNQRRQDNRRNEHRDNRNKAGRPQQGRSDQPDKKPEPR
                     DKGVDRPESIDRNQEKSNNQNKRNKKHRNQQRRSGNRPNNGNAAPPADA"
     gene            1009..2409
                     /gene="lpdA"
                     /locus_tag="PLG24_11350"
     CDS             1009..2409
                     /gene="lpdA"
                     /locus_tag="PLG24_11350"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HMZ25533.1"
                     /translation="MTYDVVVIGSGPGGYICAIRCAQLGLKTAIIEKYKSLGGTCLNV
                     GCIPSKALLDSSEHFFNAAHQMADHGINIGKVEMDAKKMFERKKNVVDTVSKGVSYLM
                     SKNKIDVHYGVGSFIDSSNIKVNGEEGESTLTSKYFVIATGSKPITPAAFNYDKKRII
                     TSTEALVLGEIPKSMVIIGAGVIGLELGSVYARLGAKIDVVEFTDKIIGTMDVDCGKE
                     LQKVLKKEGFTFHLNHAVTTVKSTKTKVTVEFKQNGTDEVKKIEADYCLIAIGRRPYT
                     DGLNAEAAGVIVDAKGRVEVGDHMRTNVPNIFAIGDVVRGAMLAHKAEEEGVFVAETI
                     AGMRPHINYNLIPGVVYTWPEVASVGETEGQLKERGVDYKVGKFPFKALGRAKASGDE
                     AGFVKVLADKNTDEILGVHMIGPRAADMIAEAVVAMEYRASAEDIGIISHAHPTYTEA
                     FKEAALDATGKRPLNI"
     gene            complement(2806..>3475)
                     /locus_tag="PLG24_11355"
     CDS             complement(2806..>3475)
                     /locus_tag="PLG24_11355"
                     /inference="COORDINATES: protein motif:HMM:NF017475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HMZ25534.1"
                     /translation="IIVPIALAFNFFFPVVIKYILIAGGCYLAYEGAEKIIEYFFHRN
                     KGHHIHQETDHNIEEDSEKKKIKAAITTDFILSIEIVIIALGTVLEKDLVIQVLTVSF
                     VAILATIGVYGLVAMIVRMDDAGYFLIRHSKSNSWRSKIGNLLVKALPYVIKMLSVVG
                     TIALVAVSGGIFVHYIDYFHHLWPKFPILLKELTFGITIGMVLVLILSSAKWIGKKLK
                     KTPA"
BASE COUNT         1100 a          703 c          816 g          856 t
ORIGIN      
        1 atagggcgga tctcaagaaa gccacattct attatacagc agacggaaga gttgactttc
       61 gggaactgat cagacatttt gccaaggaat tcagagtgaa aatagagatg cggcagatcg
      121 gttctcgtca ggaatcgtca aggataggag gcatgggctc ctgtggtcgg gaattgtgct
      181 gttcgacctg gttgacagat tttaagtcgg tcacaaccaa tgcggcacgc tatcaaaacc
      241 tggccatcaa tcaggccaaa ttgtccggac aatgcggacg cctgaaatgc tgtctcaatt
      301 acgagctcga tgtatatatg gaggccctgg aggacttccc tactaatgct gacgtgctca
      361 ggtatcaggg gggacaggcc aggctcatca aaacggatat cttcaaaaaa ttgatgttct
      421 atatcaagaa agatgaaaaa ggaagggagg cgtttatcac actgagagta gacgaagtac
      481 ttaggattca ggaagatatt gcccatggca aattgcctga agacataaca tctaccaagg
      541 tggaagcctt tgaggatgat gctgttatca aatctgaaga tctgacagga catattgaat
      601 tgcccgaaga gaagagaagg aagaaaaaga aaaagaagaa tcccgagaac aagcctatga
      661 ggcacgaagg acagaaacag gaggataaaa aagaaaccgg caatgaaggc aaggataaca
      721 atcaacggag acaggataac cgcaggaacg aacatcggga taatagaaac aaagcaggaa
      781 ggcctcaaca aggcaggtct gaccaaccgg ataaaaaacc ggaaccacgc gacaaaggtg
      841 tagacaggcc tgaatccatt gaccggaatc aagaaaaatc caataatcag aataagagaa
      901 ataagaaaca cagaaatcaa caacgcaggt ccggtaatcg ccccaacaac ggcaatgctg
      961 cgcctcctgc cgatgcttaa tttgaataaa ataaattaaa agataaacat gacttacgac
     1021 gtagtagtaa ttggttcagg cccgggtggt tacatctgcg ccattcgttg tgcccagttg
     1081 ggcttgaaaa ctgccatcat tgaaaaatac aagtcactgg gaggcacttg ccttaatgtc
     1141 ggctgtatcc catctaaagc actgttggac tcatcggagc attttttcaa tgctgcacat
     1201 cagatggcag atcacggcat caatatcgga aaagtggaga tggacgccaa aaagatgttc
     1261 gaacgtaaga aaaatgtcgt ggatacagtc agcaaagggg tgtcctatct catgtccaag
     1321 aataaaattg atgtacatta tggagtaggt tccttcattg attcctcaaa tattaaagta
     1381 aatggagagg aaggagaatc cacactgacg agtaagtatt ttgtcatcgc gaccggctcc
     1441 aaacctatca caccagcagc attcaattat gataagaaac gaatcattac ctccactgaa
     1501 gcgcttgttt taggtgaaat acctaagagt atggttatca tcggtgcagg cgtgatcggc
     1561 cttgaactgg gatcagtata tgcccggctc ggagcaaaga ttgatgttgt tgaatttacg
     1621 gataagatca taggtaccat ggatgtcgat tgtggaaaag aattgcagaa agtccttaaa
     1681 aaggaaggat ttactttcca ccttaatcat gccgttacta ctgtcaaatc caccaaaacc
     1741 aaggtaaccg tagagtttaa gcagaatgga acggatgaag tcaaaaagat agaagctgat
     1801 tattgcctga ttgcaattgg aagacgtccc tatacagatg gcctgaatgc cgaagcagcc
     1861 ggtgttatcg tcgatgctaa aggtcgggtc gaggtgggcg atcacatgag aaccaatgtg
     1921 cccaatattt ttgctatcgg cgatgtagta agaggtgcta tgcttgccca taaagctgaa
     1981 gaagaaggtg tctttgtggc agagaccatt gccggcatgc gaccacatat caattataac
     2041 ctgattcccg gtgtggtata tacctggccg gaagtagcct ccgtgggcga gacagaggga
     2101 cagctgaaag aacgcggtgt ggattataaa gtcggtaaat ttcctttcaa agcattggga
     2161 agggcgaagg caagcggtga cgaagccggc tttgtcaagg tgctggcaga taaaaatacg
     2221 gatgaaatcc tcggcgtaca catgataggc ccaagagctg ccgatatgat tgcagaagcc
     2281 gttgttgcca tggaatacag agcctcagca gaggatatag gcattatttc tcacgcacac
     2341 cctacctata ccgaagcatt taaggaagct gccctggacg ctacaggaaa gcgtcctttg
     2401 aatatataat tttttgagga gggtcaatag ttagtgagct aaatggtgta gctgccccgt
     2461 ttgctttgca taagcttaca tcgcattaat cctcgttatc catgaaccgg actttattcc
     2521 ggaagtattg aatttcattg ctatttcaat ggatagctaa cgatatctta taccaaggtg
     2581 atttataaat tgtccataag tttttggtat ccttgtccgg acagctatac taccagagct
     2641 ctgctctgtc ccgggagtga aacaatccgg ggttccggga tgccggggtt atagtcgtat
     2701 agatcaatga aattggtcaa taacaaatgt acctttggta ttatatggca gataccattt
     2761 ttgcaaaagg gatagggctt tgtttcttcc ccttttgcaa tctcatcatg ccggagtttt
     2821 tttcagtttc ttaccaatcc atttggcact tgatagaatc aataccaaca ccattccaat
     2881 cgtgataccg aaagtgagtt ctttcaaaag aatcggaaac tttggccaca aatggtgaaa
     2941 ataatcgatg taatgtacaa atatccctcc cgatactgct acaagtgcaa tggttcccac
     3001 tacggaaagc attttgatga cgtatggcaa tgctttgaca agcaggttac caattttgga
     3061 cctccaactg tttgatttag aatgtctgat caggaaatat cctgcatcat ccatccgtac
     3121 aatcatggcg accagaccat acacaccgat agtagccaaa atagccacaa agctgactgt
     3181 taggacctga atgacaagat ctttttccag aaccgttccc aaggcaataa ttacaatctc
     3241 tatggaaagg ataaaatcgg tggtgatggc ggccttaatc ttctttttct ctgaatcctc
     3301 ttcgatatta tgatcagttt cttgatggat gtgatgacct ttattcctgt gaaaaaaata
     3361 ttctattatt ttttcagcac cctcatatgc caggtagcaa ccacccgcaa tcagaatgta
     3421 cttgataact accgggaaaa aaaagttgaa agcaagtgca atgggcacaa taatc
//
LOCUS       DAOQQN010000348         3428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_13714_length_3428_cov_4.745331, whole genome shotgun sequence.
ACCESSION   DAOQQN010000348 DAOQQN010000000
VERSION     DAOQQN010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13714_length_3428_cov_4.745331"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1784
                     /gene="ettA"
                     /locus_tag="PLG24_11360"
     CDS             114..1784
                     /gene="ettA"
                     /locus_tag="PLG24_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HMZ25535.1"
                     /translation="MADHKIIFSMTGVSKIIPPNKTILKDIYLSFFYGAKIGVLGLNG
                     AGKSTLLKIIAGIDPNYNGKIVFDKDYKIGYLEQEPTLDPEKTVLDVVKEGVQEVVDL
                     LKEYEEVNARFAEELSDDEMNQLIEKQGELTDKLEQRDAWNLEYKLEMAMDALRLPDP
                     AASISVLSGGERRRVALCRLLLSEPDILLLDEPTNHLDAESVDWLEQYLHNFPGTVIA
                     VTHDRYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKSNRLAQEEKSESKRQKVLKRE
                     LEWTRLAPKARHAKSKARLTAYEKLAEESASEKEAKLELVIPPGPRLGDQVIDIEGVS
                     KSFGDRTLYENLNIRIPRNAIVGIIGPNGVGKSTLFKMITGELQPDSGQIVIGESVKI
                     AYVDQSHKDLKPESSVYDAVSGGLDNLIVNGMTVNSRAYISKFNFSGTDQSKKVGVLS
                     GGERNRLHLAIALKEGGNVILLDEPTNDIDVNTLRSLEEALENFAGCVLVISHDRWFI
                     DRLATHIISFEDEGVYFFEGNYTDYEENKKTRLGEVKRKSKFKNLLSI"
     gene            1902..2801
                     /locus_tag="PLG24_11365"
     CDS             1902..2801
                     /locus_tag="PLG24_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HMZ25536.1"
                     /translation="MKKKTLLVLAAGMGSRYGGLKQLDGLGPNGETIIDYSVYDAVRA
                     GFNKLVFVIRPHFEEEFKEKVSSKYTSLAEVVHVHQEIDVPIEGVDSFPKREKPWGTG
                     HAVLVCEHAIKEPFAVINADDYYGKDAFAQMADFLDNRVAADNNALIGYKLANTLSEN
                     GTVSRGVCQVDEQLQLVTINERTKIGPENGRILYVDGNEKVEVPADSFVSMNFWGFHP
                     DVFPLFRKGFVHFLKYSNPTEKSEYFIPLVVDMMIKMKLSRFEVIPSNSEWIGVTYQE
                     DREPTVRKFAEFGKQGIYPEELF"
     gene            3154..3345
                     /locus_tag="PLG24_11370"
     CDS             3154..3345
                     /locus_tag="PLG24_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HMZ25537.1"
                     /translation="MQEGKVKFYNETKGFGFIKPNDGSADIFFHVSGVKEDVKDNDPV
                     VYDVEQGKKGLIAVNIRLA"
BASE COUNT         1008 a          628 c          850 g          942 t
ORIGIN      
        1 agcctttgtt atgaatctgt ccttgaaatg aggtacagag atgaaatgtt ttggccggat
       61 tcgaattgtt accttttctt tgcttacttt tgcgcaaaaa tcaaaaaagc taaatggcag
      121 accataagat catcttctca atgaccggag tgagtaaaat cattcccccc aataaaacca
      181 tccttaagga tatatatctt tccttctttt atggagctaa gattggtgtc ttgggtttga
      241 acggtgccgg aaaatccacc ttgcttaaaa ttatagcggg tatcgaccct aattacaacg
      301 gcaagattgt attcgataag gactataaaa tcggctatct cgagcaggaa cctacccttg
      361 atcccgaaaa gactgttttg gatgtcgtaa aggagggagt tcaggaagtt gtggacctat
      421 tgaaggagta tgaagaggtg aatgcccggt ttgcggaaga gttgagtgat gatgagatga
      481 atcaactcat cgaaaagcag ggagagctga ctgataagct ggaacaacga gatgcatgga
      541 atctggagta caaactcgaa atggcgatgg atgccctacg cctgccggat cctgcggctt
      601 ccattagcgt attatccgga ggggagagga ggagagtggc attgtgtcgc cttttgttga
      661 gtgagccgga catcctttta ctggacgaac ctaccaacca cctcgatgcg gaatcggtgg
      721 actggttgga gcaatatctc cacaacttcc cgggtacggt tattgctgtc acgcacgacc
      781 ggtatttcct ggacaatgtc gccggatgga tcctggagct tgatcgtggt gagggcattc
      841 cctgggaggg gaattatagt tcctggttgg agcaaaagtc caatcgactt gctcaggaag
      901 aaaagtctga atcaaaacgt caaaaagtac tcaagcgtga attggaatgg acgcgattgg
      961 cccctaaggc ccggcatgcc aagtccaagg cccgtctgac tgcctacgaa aaacttgccg
     1021 aagaatctgc cagtgagaag gaagccaaac tcgaactggt cataccgccc ggtccgcgat
     1081 tgggagacca ggttattgat atcgaaggtg tgagtaagtc atttggtgat cgaaccttat
     1141 atgaaaacct taatatcagg ataccaagaa acgctattgt cggtatcatc gggccaaatg
     1201 gagtaggaaa gtcaactttg ttcaagatga ttaccggtga gttgcaaccg gatagcgggc
     1261 aaattgtaat cggagaatct gttaagattg cctatgtaga tcagtcccat aaggatctga
     1321 agcccgaaag cagcgtatac gatgctgtat caggtggcct ggacaacctt attgtcaacg
     1381 gtatgaccgt aaactctcgg gcctacatca gtaagtttaa tttcagtgga accgaccaga
     1441 gtaagaaggt cggagtgctg tcaggcggtg agcgtaacag gctccatctg gccatcgctt
     1501 tgaaggaagg tggcaacgtc atcctccttg atgagcctac caacgatatt gatgtgaata
     1561 ctcttcgttc gttggaggaa gcattggaga attttgccgg gtgcgttctt gtaatctctc
     1621 acgatagatg gtttatcgat cgtcttgcta cacacattat ttcatttgaa gatgaaggtg
     1681 tgtacttctt tgaaggtaat tataccgact atgaggagaa taagaagaca cgactcggag
     1741 aagtcaaacg taaatcgaag ttcaagaatc tcctgagtat ataatcgtaa ggagttattg
     1801 atagatcgta gttgaattca gggctttaca tcctgaggct ttatgtttta aatcaaatag
     1861 gaattgttaa ttttcaaaca ttggattaga aattataaga catgaaaaaa aagacactgt
     1921 tggtactggc agccggaatg ggttcacgtt atggggggtt gaagcaattg gatggattgg
     1981 gtccaaatgg tgagaccatc atcgactatt cggtgtatga tgccgtcagg gcaggcttca
     2041 acaagctggt tttcgttatc cggcctcatt ttgaggagga attcaaggaa aaagtgtctt
     2101 ccaaatatac atcattagcc gaggtggtcc atgtgcacca ggagattgat gttcctatag
     2161 aaggtgtgga ctcttttcct aaacgggaaa aaccatgggg caccggacat gccgtcctgg
     2221 tatgcgaaca tgccattaag gagccctttg cggtgatcaa tgccgatgat tactatggta
     2281 aggatgcatt tgcccagatg gcagactttc tggacaatag ggtagctgct gataataatg
     2341 ctctgatcgg gtacaaactg gccaatacct tatccgaaaa tggcactgtg tcgcgcggtg
     2401 tatgccaggt ggatgaacaa ttacaacttg tgaccatcaa cgaaaggacg aagattggcc
     2461 cggagaatgg ccgtatcctt tatgtcgatg gcaatgaaaa ggttgaagta ccggcagact
     2521 cctttgtttc catgaatttc tggggcttcc atcccgatgt attccctctt tttagaaagg
     2581 ggtttgtcca cttcctgaaa tattccaatc ctacagaaaa gtctgaatat ttcatacctt
     2641 tggtggtcga tatgatgatc aagatgaagt tgagtcgttt tgaagtcata ccttccaaca
     2701 gtgaatggat cggtgtgact taccaagaag accgggagcc gactgtcaga aagtttgcag
     2761 aatttggaaa acagggtatt tatcctgaag agttgttctg aggaatcccc cgattataat
     2821 tcttaactca tatagcctgg tattagagat ctatatacct gtaattgtat tgtttcattg
     2881 aaattgaatg gaaacaatag cgcgtagtac gatttgtaca aatgaaaaaa caggattaga
     2941 aaaattaagt tttgtgtggt acctcccttg tataattaag cagttcagac gatgaattaa
     3001 taaaaaacag gaaagtatta aaagttggta agtttggtgt gcagttgcac ttttaatgag
     3061 ccaaactccg tttgagtgaa gtaaaattca acgacgatgc tgatacccat gaaattgtaa
     3121 attaataaaa tattaaacat taaatgaatt attatgcaag aaggtaaagt aaagttttac
     3181 aacgaaacaa aaggttttgg ttttattaaa cccaacgatg gttctgctga cattttcttt
     3241 cacgtttccg gggttaagga agatgttaaa gataatgatc cggttgtata tgatgtcgag
     3301 caaggtaaaa agggattaat tgctgtcaat ataaggttgg cgtaaggtag aactaaatac
     3361 gtcttttagc aggttattgg gatgattgat tctgtgtcca cgacacggat atcagacgtt
     3421 ccaattat
//
LOCUS       DAOQQN010000349         3413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_13808_length_3413_cov_4.410959, whole genome shotgun sequence.
ACCESSION   DAOQQN010000349 DAOQQN010000000
VERSION     DAOQQN010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13808_length_3413_cov_4.410959"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..771)
                     /gene="deoC"
                     /locus_tag="PLG24_11375"
     CDS             complement(124..771)
                     /gene="deoC"
                     /locus_tag="PLG24_11375"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HMZ25538.1"
                     /translation="MQLNKYIDLTYLKPDSTRQRIKELCTEAIENDVFSVCIPPYYIP
                     MAAELLEDHSVKVCTVIGFPYGYDATPTKAEAVKKAVVEKVDEIDVVVNIAAIKNGDW
                     SYVANDVDSITRLGHMRNLLVKLIIEVNLLTEDEIKRICDIAVQKEVDFIKTSTGTVG
                     DAVTVEDIILLRSLIPATTKVKASGGIRTREQAMALIDAGADRLGTSNVSSILLP"
     gene            complement(1053..>3413)
                     /locus_tag="PLG24_11380"
     CDS             complement(1053..>3413)
                     /locus_tag="PLG24_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HMZ25539.1"
                     /translation="ISGGVVDSISKQEIEFVSVAVYRQGNSTPVDGALTDKSGMFNIK
                     ELKNGVYDVHINFLGYQNKVISNIKVSDSIPDQMLGKIYISPESKLLNEVVVTGEQGL
                     VENKIDRMVYNAGKDISNKGGNAADVLRKVPTLSVDLDGNLSMRGSSSIRVLINGKPS
                     AVMASSVADALKMIPSDEIDKVEVLTSPGAKYDAEGSAGIVNIITKRKTIKGFSGNVT
                     ISAGTRSAFGGANANLKLDKIAFTAGVGGFGWRAKGNLNSERSAPNYLLTQTGRNNMS
                     GLGGRGNIGIDYDITEKDNINATLSFGKFGMNLDNSITSDQMLAGILNQHYRRDQTSV
                     NDNLSTDINLDYKHKFKKENQELTISGQYSNNNRPTHYEISQYNDAEILDYKEFSNNK
                     GTNREITAQADYSHPFSKKLSLDLGIKSISRRIESYYVYDTFNLATNTQIKDEMRSNS
                     FGYLQNVLAGYAEATWAITPKFGLKAGVRYEGTTVEGDIFKEVQSLNNNYYNILPSGT
                     FSYKWGSNGLKLSYNQRLQRPSLFYLNPYSNLADPKNITVGNPKLNAELSHNVEVGYN
                     KFFGLSSVNIAVYRRFTNNAIEAVRKISGDTIVTTYYNQANNKNTGLNVSGNLMKGYK
                     FMIGGNFDISYVEVDNNALGINNSGINYGINGFLNWTIYEAWGIQAYGGFRGPTITSQ
                     GKSTSFYFYGIGAKRDLLNKKASLSIGVDNPFTPYQVNKTELNVYGTQFKSENRFYAF
                     GGRISFNWMFGKMSFSNKSNEKGIQNDDLKSGENGQGPGGQMGRMK"
BASE COUNT          871 a          751 c          706 g         1085 t
ORIGIN      
        1 ggttgttgtt gtactctttg actgcagttt ctgtatttga atccatggtg attgcaacct
       61 gctgggcata acccaggtga cataagaaaa taaaaaacag gaagcagaga caggcttttc
      121 gcatcaaggt aataaaatag atgaaacatt agatgtgcct aatctgtctg ctccggcatc
      181 aatcagggcc atggcctgtt ctctggtccg aatgccaccg ctggccttga ccttagttgt
      241 ggccggaatc aaactgcgga gcaggattat gtcttctaca gtgactgcat caccgaccgt
      301 accggtactt gtcttgataa aatccacttc cttctgtact gcaatatcac atattctttt
      361 gatctcgtct tccgtaagca ggttgacctc aatgattagc ttcaccagca ggttgcgcat
      421 atgtcccaag cgggtaatgc tgtccacatc attggcgaca taggaccaat ctccattttt
      481 gatggctgca atattgacaa cgacatcgat ttcatctact ttttccacga ctgctttctt
      541 gactgcctca gccttggtcg gtgtggcatc ataaccatat gggaatccga taactgtaca
      601 cactttaaca ctgtgatctt ccagcaactc agcagccata ggtatgtagt aaggcggaat
      661 acagacagag aatacatcat tttctatagc ctcggtacat aactccttaa tacgctgcct
      721 ggttgaatcc ggtttaagat aggtcaggtc tatgtattta tttaattgca tattggattt
      781 cgattttcgg aatcacaaag ataataaaat tagccacagc ggaaaatcaa atcacagcca
      841 gaattagtat tgatttatga taatcaattg aaaatgtgta aattttacaa ttatatttca
      901 atggaatgtt ccttgaagaa aattttttta gtcgatgccg taaaaaatgt aaaaagacga
      961 attgtggttt gttctaaaac tgccaaatca caattcgcct caggatgaag gtcttacctt
     1021 gatggtaaca taacctgccg tgccgttctg tcttatttca ttcgccccat ctgccctccc
     1081 ggaccttgac cattttcgcc tgatttaagg tcgtcattct gaataccttt ttcattggac
     1141 ttatttgaaa aactcatctt tccaaacatc cagttgaagc tgatacggcc gccgaatgca
     1201 tagaatctgt tttctgactt gaattgtgtg ccatacacat ttaattcggt tttattgacc
     1261 tggtaagggg taaaaggatt gtcaacacct atagacagag aagctttctt gttgagcagg
     1321 tcgcgttttg caccgatgcc ataaaaataa aagctcgttg atttgccttg tgaggtgatg
     1381 gtcggaccac ggaagccacc gtaagcctgg atgccccacg cttcatagat ggtccagttg
     1441 agaaatccat tgataccgta attgataccg ctgttgttga tgccgagggc gttattatct
     1501 acttcgacat aagagatgtc aaaattgcca ccaatcataa acttgtagcc tttcatgagg
     1561 ttaccggata cattgagtcc tgtgttttta ttgttggcct ggttataata ggtggtgacg
     1621 atggtgtctc cactgatttt cctgacggcc tcaatagcat tgttggtaaa acggcgatat
     1681 acggcaatat tgactgagct gagtccgaaa aatttgttgt atcctacttc cacattatga
     1741 gacaattcgg cattcaattt tggatttcca actgttatat tcttcggatc ggccagattg
     1801 gaataaggat tcaggtagaa taaagatggc ctttgcagtc tttgattata ggaaagcttg
     1861 agaccgttac ttccccattt gtaggagaaa gttccgcttg gcagtatatt gtagtagtta
     1921 ttattcaggc tttgaacctc cttgaagatg tctccttcca cagtggtccc ttcatatcta
     1981 acacctgctt ttaacccgaa tttgggcgtg atggcccagg ttgcttcagc ataacccgcc
     2041 aacacgtttt gcaaataacc aaaggaattg gagcgcattt catccttgat ttgggtatta
     2101 gtagccagat tgaaggtgtc atatacataa tagctttcaa tccttctcga tattgattta
     2161 ataccaagat caagactcaa cttcttggag aatggatgtg agtaatccgc ctgagctgtg
     2221 atttccctgt tagtgccctt gttgttgctg aattctttgt aatctaaaat ttcagcatca
     2281 ttatactggc tgatttcgta gtgggttgga cggttgttgt tgctgtactg tcctgagata
     2341 gtcaattcct gattttcttt tttgaatttg tgtttatagt ccagattgat atcggtgctc
     2401 aaattatcgt taacacttgt ttgatccctt cggtagtgct gattcagtat acctgcgagc
     2461 atctggtcgg aagtgatgga gttgtccagg ttcatgccaa atttgccaaa gctcagggtg
     2521 gcattgatat tatccttttc cgtgatgtca taatcaatac ctatattacc tctgcctcca
     2581 aggccactca tgttgtttcg gccggtctgg gttaggagat agttaggtgc agaacgctct
     2641 gagtttagat tcccttttgc acgccaacca aagccgccaa cacccgccgt gaaagctatt
     2701 ttatcaagtt tgaggtttgc attggcaccc ccgaaagcac ttcttgtgcc ggcagaaatt
     2761 gtaacatttc cgctgaatcc cttgattgtc ttgcgtttag tgatgatgtt tacgataccc
     2821 gcgctccctt cagcatcgta tttagctccc gggcttgtga gtacttctac cttgtctatt
     2881 tcatcggaag gtatcatctt aagggcgtct gctacgcttg aggccatgac tgcagaaggc
     2941 ttgccattga tcagcacccg aatgctgcta cttccgcgca tagacagatt accatccagg
     3001 tcaacactta gggttggtac ctttctgagt acatcggctg cattgcctcc cttgttggat
     3061 atatcctttc cggcgttata caccatacga tctattttat tctctactaa tccctgctcc
     3121 ccggttacca cgacctcatt gagcaacttc gattccgggc tgatgtatat cttgccaagc
     3181 atttgatccg ggatggaatc actcaccttg atattcgaaa taactttatt ctggtaaccc
     3241 aggaaattga tatgaacatc gtaaacacca tttttcaatt cctttatatt gaacatacca
     3301 cttttgtctg tcagtgctcc atctaccggt gtggaatttc cttgacgata gactgccaca
     3361 gatacgaatt caatttcctg tttgctgatg ctatcaacaa caccaccgga tat
//
LOCUS       DAOQQN010000350         3396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_13914_length_3396_cov_5.152649, whole genome shotgun sequence.
ACCESSION   DAOQQN010000350 DAOQQN010000000
VERSION     DAOQQN010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13914_length_3396_cov_5.152649"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="PLG24_11385"
     CDS             <1..1330
                     /locus_tag="PLG24_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25540.1"
                     /translation="IPVSITKLTLLNNVVKNTNLALADNNNIKENYSVRVPHDIPLTT
                     PYWLQGNSSPMMYDVSDRDIIGRPENIYPLDGRLSIDVAGYAITIPIRILYKNVEPAY
                     GQRYGEVQVVPPVTVNIDRNCILAHPTGKTSLHCTVRGFVSDISARLYPDLPKGWTSE
                     PPYFDIGMKLSGESRDYKFMISGPAFAGIDSVGAHIEWKGKHYNRSFQEIRYDHVPYR
                     IINIANKVPVSIVAPPKSKELIGYIDGAGDLVFESLKAAGYNIERIPSDQLNESQLAK
                     YRTILVGIRAFNVLENAAVLNQALSEYAANGGRVVVQYTTAGGMKVKQIGPYPFSVSR
                     TRVTDESADVHFLLPEHILVNKPNKITTDDFRYWVQERGLYFGDKYQDNYQALFGMHD
                     KNENEARGSLLYAPVGKGSFIYTGLVFFRELPAGIPGAYRLMENLLYNPQ"
     gene            1443..3134
                     /locus_tag="PLG24_11390"
     CDS             1443..3134
                     /locus_tag="PLG24_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HMZ25541.1"
                     /translation="MRELDWVVLVATLLFITFYGIAKNLRNQTAESYVVGTRDMKWWA
                     ICISIMTTQASAVTFISTPGQGFVDGMRFVQFYFGLPLAMIVLSAFFVPLYYKMRVQT
                     AYEYLEHRFDLKTRSLTSVLFLIQRAMGAGLTIYAPAIVLSVVMGWDLKVTIVALSIL
                     TIAYTTLGGYKAVAQTQQYQMVVIFLGLFLTFFILLYSMPKGIGLSESLALAGQSNKM
                     QLVDFRFNPEDRYNIWSGIFGGFFLFMSYFGTDQSQVGRYLSGASIRESRLGLLFNGL
                     LKIPMQFFILLLGVLVFVFFIFNKAPLHFNPVNTQIVETGLYKSQWNDLNRRYDELFH
                     LRQSAYKQSLESNDKSTAVERYEEQLRGLRAEADSLIYLSNPALDVNDKDYVFISYVL
                     RYLPVGMVGLLIAVIFSSSMSSKASELNALASTTIVDLYRRSIRKGRSDKHYLSATKW
                     STVIWGILAMLFAMFTSLFENLIQAVNIVGSLFYGAVLGIFAVAFFFRKIGGNAVFWA
                     AIAAEGIILSLYVLGEMKVLSIGFLWYNLIGCVLVIAFGMVFQAIDNRKSANRKE"
BASE COUNT          873 a          666 c          830 g         1027 t
ORIGIN      
        1 aatacctgtg agtataacta aattgaccct tttgaacaat gttgtgaaga ataccaatct
       61 tgctcttgcc gataataata acatcaagga gaattactcc gtccgggtac cccatgacat
      121 cccattgaca acgccttatt ggttgcaggg caattcctcg ccgatgatgt atgatgtcag
      181 cgatcgggac atcattggaa gaccggagaa tatatatcca ttggacggaa ggttgagtat
      241 cgatgtcgcc ggatatgcca ttacaattcc cataagaata ttgtataaga atgtcgagcc
      301 ggcttatgga cagcgatacg gtgaagttca ggtggtgccg cctgtcacag tgaatatcga
      361 ccggaattgt atccttgcac atcccacggg caaaacgtca ctacattgta cggtcagggg
      421 gttcgtgtct gatatcagtg cgagattata tcccgactta ccaaaaggat ggacatcgga
      481 gccgccctat tttgatattg gcatgaaact cagtggagag tcaagggact ataaattcat
      541 gatttcaggt cctgcatttg ccggtattga ttccgttggt gctcacatag aatggaaggg
      601 taagcattat aatcgcagtt ttcaggagat caggtatgat catgtccctt accggattat
      661 taatattgca aacaaggttc cggtttccat cgtggccccg ccaaaaagca aggagttgat
      721 tggctatatc gacggagccg gtgatttggt ttttgaatcg ttaaaggcag caggctacaa
      781 tattgaaaga attccttcag accagttgaa tgaatcccaa ttggcaaaat acagaactat
      841 tttagtgggc atccgcgcat tcaatgtact tgaaaatgcc gctgtgctca atcaagcatt
      901 gagtgagtat gctgcaaatg gtggcagggt agtggttcag tatacaacag caggtggtat
      961 gaaggtcaaa caaatcggac cttatccttt tagtgtgagt cgaaccagag ttactgatga
     1021 gagcgctgat gttcattttc tgcttccgga acatatcctt gttaataagc cgaataaaat
     1081 cactacggat gacttcaggt actgggttca ggaacgaggt ctttattttg gagataaata
     1141 ccaggataat tatcaggcat tgttcggaat gcatgacaaa aatgaaaatg aagccagggg
     1201 aagtttactt tatgcacctg ttggcaaagg ttcatttata tataccggcc tggttttctt
     1261 tagagagctt cctgccggta tccccggagc ataccgattg atggagaatt tgttatacaa
     1321 ccctcagtga tcaggatgaa ggaagatcaa accaactgga acaaggcgta catatgggtg
     1381 tttgctgtga tggtgttcac catatttgtt ttttggctga ttcaaataaa ttataaccac
     1441 gcatgaggga gctggactgg gtagttttgg ttgcgacctt gttgtttatc acattttacg
     1501 gtattgcgaa gaatcttcgg aaccaaacag ccgagtcgta cgtcgttggc acccgggata
     1561 tgaagtggtg ggctatctgc atatcgatca tgacgacaca ggccagtgca gttaccttca
     1621 tatctacgcc cggccagggt tttgtcgacg gcatgcgctt tgtgcagttc tattttggcc
     1681 tgccgcttgc aatgattgtt ttgtcggctt tctttgtgcc actttattac aagatgcgcg
     1741 tacagacagc ctatgaatat ttggaacatc gcttcgacct gaagaccaga agcctcacgt
     1801 ctgtgttgtt tttgatacaa agggccatgg gcgcaggcct gaccatatat gcacctgcaa
     1861 ttgtcctgag tgtggtaatg ggctgggatc tgaaggtgac tattgtagcc ttgagcattc
     1921 tgaccatagc ctatacgaca ttgggtggct ataaggcggt ggctcagacg caacagtatc
     1981 agatggtcgt catcttcctg ggactctttc ttactttttt tattttgctt tatagcatgc
     2041 caaagggtat tggattgtct gaaagtctgg cattggcagg gcaaagtaat aaaatgcaat
     2101 tggttgattt taggtttaac cccgaggata ggtacaacat ctggtcgggt atattcggag
     2161 gcttcttcct ttttatgtct tattttggca ccgatcaatc acaggttgga cggtatctct
     2221 caggagctag tatccgggaa agcaggctgg gattgctttt caatggactc ctgaagattc
     2281 cgatgcagtt tttcatttta ctgctaggtg tgcttgtgtt tgtatttttt atttttaaca
     2341 aggcgcccct gcatttcaat ccggtgaata cgcagattgt cgaaaccgga ctttataaaa
     2401 gccagtggaa cgacctaaac cgacgctatg atgaattgtt tcatttgcgg caatcggctt
     2461 acaagcaaag tcttgaaagc aatgacaaat caactgcggt ggaaaggtat gaagagcagt
     2521 tgagaggatt gagagcagag gctgacagct tgatttatct ttctaatccg gcactggatg
     2581 tcaatgacaa ggattatgtt tttatttcct atgtgcttag atatcttcct gtcggcatgg
     2641 ttggacttct gattgctgtt attttctcca gcagcatgtc gagtaaggct tctgaactga
     2701 atgcacttgc ctcaacgacc attgtggatc tgtatcggcg ttctatccgc aaaggtcgat
     2761 ctgacaagca ttatttatca gctactaagt ggagtactgt tatctggggc attctggcca
     2821 tgcttttcgc catgtttacc agtttgttcg agaatctgat tcaagcggtg aatattgtag
     2881 gctcactctt ttatggtgca gtcctcggca tatttgccgt tgccttcttt ttcaggaaaa
     2941 taggagggaa tgctgtattc tgggcggcca ttgctgctga ggggattatc ctttcgctgt
     3001 atgttctagg ggaaatgaag gtattgtcca ttggatttct ctggtataat ctgataggat
     3061 gtgttctggt tattgccttt ggaatggtct ttcaggctat agacaatcga aaatcagcca
     3121 ataggaaaga ataaaatatt gcgccggtaa aaatctatgg aggatgcccg gctgatttat
     3181 gttgcaagta agacgctcaa ttcctgcttg taagctatac tgactaaccg gctttgaaaa
     3241 ttattgaacg ttattttcta tcagttctat tctgtatata ctcggccaca gcttgcccgt
     3301 gaggtatatc ttattggacg tagaatcata ggcgatgcca ttggcgacct cgctgtattt
     3361 ccaggtcgca ttggcttcat gtttcaaact tgaaac
//
LOCUS       DAOQQN010000351         3385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_13990_length_3385_cov_5.234535, whole genome shotgun sequence.
ACCESSION   DAOQQN010000351 DAOQQN010000000
VERSION     DAOQQN010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13990_length_3385_cov_5.234535"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PLG24_11395"
     CDS             <1..530
                     /locus_tag="PLG24_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HMZ25542.1"
                     /translation="AVILQDYEKGMLHHANIPAFIEFLNECGIRIIVDPKDKNFWMYR
                     HVHLFKPNRKEAEKAIGKKIPMTEDALADAALLLESRLHNEVTVITLSEYGIYIKDAE
                     NNVWRHSEVMEVIDVCGAGDAVIGIVAAAYCAGLPMPQIALLGNIAGGIVCGERGVVA
                     VSLDRLQKAYNNMSL"
     gene            634..3033
                     /locus_tag="PLG24_11400"
     CDS             634..3033
                     /locus_tag="PLG24_11400"
                     /inference="COORDINATES: protein motif:HMM:NF014301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ25543.1"
                     /translation="MRKFLTLKVSMSFWMLLGFLSVGNIAMAQSFYIEFLSPAEIRGQ
                     RVNFVAGASTVVCHLPFGDSLNTQLTDPILAGDPNDPNNTQFTFAEALNGNVGVCKRG
                     SFSFNVKASNIEFNGGSGSIIINNDQANKDAIISMSQDTSFSIWSVAVSFNSGENFFL
                     KYPGLSFKFVNDFPAETDNILWSDNGFSGGLDGWTTVGVACGDGKDPVGAEWEWTEFG
                     SSKGAYGSTNILSATACNGAMTFNTDFKDNDGVQGNFGGGICGVNQIGELISPDIDLS
                     ALPEETAISLRFNQAVRQYQSRFFVSWSNDGGANWHDIEVNKDLVINDNPTNGFYRLK
                     LGGAKPSANFKLKFTIQGNYYYWIVDDVKLIEPEDFNSLIDKDWLAGAPYATTPISQV
                     TPVKFMTDAHNIGGKSLTGMKLTATVTDEGGNEVYTASFAPTSPGQVEGTVLSGDTIQ
                     NHVFGSYTPDAAGVYTIKYDLTSDQEDFDPSNNSYSTTFTVTDDNTGEGDFSRENEVT
                     TALTLVFGDSDPINWIYANVLRGENEGSVAKRVKFGVANANDLSAGGASLYCYVWECE
                     DSDGDYQITEAERTLVGFNFHDMLESDPDELYLDLPILDFDTEEEKSVEIKKGKWYIV
                     GVAYEATSVAPRQVAYILAGGNSNQNTVEFIALAEDPASYVPTHFQMKGWNFSSTFNL
                     TPNVVTGGRGAWEQAPYARLVLDSRSSVKNLKLADNTFSVYPTNASENISVKFNFENG
                     TDVNTEIYDVNGNRVYSKKMQNQKDQTNIIETGNFVPGAYFVRVTTSNGALSKPFNVI
                     K"
     gene            3188..>3385
                     /locus_tag="PLG24_11405"
     CDS             3188..>3385
                     /locus_tag="PLG24_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HMZ25544.1"
                     /translation="MRRKIVAGNWKMNLDMNGSEELVQSILKNIAPENCSVLFFPPYP
                     FLFRVNEIIGARKNIFIGGQNI"
BASE COUNT          992 a          567 c          784 g         1042 t
ORIGIN      
        1 atgcagtaat tcttcaggat tatgaaaaag gcatgttgca tcatgcgaat atccctgctt
       61 ttatagaatt tctgaatgaa tgtgggataa ggattatagt ggatccgaaa gataaaaatt
      121 tctggatgta ccgtcatgtt catttgttta agcctaacag aaaagaggca gaaaaggcga
      181 ttggcaagaa gataccgatg acggaggatg ctctggctga tgcggcccta ctgttggaat
      241 cccgattaca taatgaagtc actgtaatta ccttatcaga gtatggtata tatattaaag
      301 atgcagaaaa taatgtctgg aggcattcgg aagtgatgga agtgatcgat gtatgcggtg
      361 ctggagatgc tgtaataggc attgtggctg cagcgtattg tgcaggccta cccatgcccc
      421 aaattgcact tttgggtaat attgccgggg ggattgtttg tggtgagagg ggggtagtag
      481 cagttagctt ggacaggttg caaaaagcat ataataatat gtccttataa aaaattaacg
      541 aaatttattt ttgagtattc aattctttcc ttataattgt gtcttaattt gtaatcattt
      601 aatcttatta cattaataaa aaagtttagt attatgagaa aatttttgac tctaaaggtg
      661 tcaatgagtt tttggatgtt gttgggcttt ctgtctgtag gcaatatcgc aatggctcaa
      721 agtttttaca ttgagtttct atcgccagct gagatcagag ggcagcgtgt taattttgtg
      781 gcaggcgcat caacagttgt atgccactta ccatttggtg attcattgaa cactcagttg
      841 acggacccaa ttcttgcggg agatccaaat gatccgaaca acactcaatt tacatttgcg
      901 gaagctctga acggcaatgt tggtgtatgc aagagaggaa gtttttcttt taacgtaaag
      961 gcctctaata ttgaattcaa tggtggtagc ggatccatta taatcaacaa tgatcaggct
     1021 aataaggatg ctatcatatc aatgtcacag gacacttcct ttagtatttg gagtgttgct
     1081 gtttcattta attcaggtga gaatttcttc ctgaaatatc ccggcctgag ctttaagttt
     1141 gtcaatgatt tccctgcaga aactgacaat attctctggt cagataatgg attcagcgga
     1201 ggtctggatg gctggaccac tgttggtgtc gcttgtggag acggcaagga tcctgtgggc
     1261 gctgagtggg aatggactga atttggctct tcaaaaggtg cttatggtag cacaaacatt
     1321 ctttctgcga cagcatgtaa tggtgccatg acctttaata ctgactttaa ggataatgat
     1381 ggtgttcaag gtaattttgg cggtggtata tgtggtgtca atcaaatagg tgagcttatt
     1441 tctccggaca ttgaccttag tgccttaccg gaagaaactg caatctccct gagatttaac
     1501 caggcagtta gacaatatca aagtagattc tttgtttcat ggtccaatga cggcggagct
     1561 aattggcatg atattgaagt aaataaggat ctggtaatca atgataatcc taccaatgga
     1621 ttttacagat tgaagctggg aggtgcaaag ccaagtgcca actttaagct taaatttaca
     1681 atacaaggta attactacta ttggatcgtt gacgatgtga agttgattga acctgaagat
     1741 tttaattcat tgatcgataa agattggctt gcaggagctc catatgcaac gactccaatc
     1801 agtcaggtta ctccggttaa gtttatgact gatgctcata atattggagg taaatcactg
     1861 actggcatga aattgacagc aacagttaca gatgagggcg gcaatgaggt ttatacggca
     1921 agctttgcac cgacaagccc gggccaggtt gaaggtacag tgttgagcgg tgataccatc
     1981 cagaatcacg tctttggctc atatactccg gatgctgctg gtgtttatac aatcaagtat
     2041 gacctgacat ctgatcagga agactttgat ccttcaaaca acagttattc caccacattt
     2101 acagtaactg atgataacac aggtgaaggt gatttcagta gagaaaatga agttactaca
     2161 gctttgactc tggtgtttgg tgattctgat cctatcaact ggatatatgc caatgtcctg
     2221 agaggagaaa atgagggtag tgtagcaaaa agggtcaagt ttggtgtagc caatgccaat
     2281 gatctgtctg caggtggagc atctctctat tgctatgttt gggaatgtga ggactctgat
     2341 ggcgattacc agattactga ggcggaaaga accttggtag gattcaattt ccatgacatg
     2401 ttggagtctg atcctgatga actatatctg gatcttccaa ttctggattt cgatacagaa
     2461 gaagaaaaat cagttgagat aaagaaagga aagtggtaca tcgtaggagt ggcatacgaa
     2521 gctacatcag tggctccacg acaagttgcg tatattcttg caggtggaaa ctctaaccag
     2581 aatacagttg aatttattgc attagctgaa gacccggcta gttatgttcc tactcacttc
     2641 cagatgaaag gctggaactt cagttctacc ttcaatctta ctccaaatgt tgtgactggt
     2701 ggtagaggtg catgggagca ggctccttat gctcgcttgg ttcttgatag ccgttcttct
     2761 gttaagaatc tgaaacttgc tgataataca ttctcagtat acccaaccaa tgcatctgaa
     2821 aacatttctg tgaagttcaa ctttgagaat ggaactgatg tgaatacaga aatctatgat
     2881 gttaacggaa atagagtgta ttctaagaaa atgcaaaatc agaaagatca gactaacatt
     2941 attgaaaccg gtaactttgt tccgggagca tattttgtaa gggttactac ttctaatggc
     3001 gcattatcta aaccgtttaa tgtaatcaag taatcattga ttgagtgaaa atgaagaagg
     3061 gcggttaaga accgcccttt ttttgtttta tccgttccga ctttggtttg ctgatttaag
     3121 tccccgattt ttcattaatt tttgaagacc attttaattc cttaattttg aactttaaaa
     3181 ttatgctatg cgtagaaaaa ttgtagccgg taactggaag atgaaccttg atatgaatgg
     3241 ttccgaggag ttagtacaat ccattctgaa aaatattgcg ccggaaaatt gctctgtact
     3301 gttttttcct ccctatcctt ttttatttag agtcaatgag ataatcgggg ccaggaaaaa
     3361 tattttcata ggagggcaga atatc
//
LOCUS       DAOQQN010000352         3344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_14262_length_3344_cov_4.921557, whole genome shotgun sequence.
ACCESSION   DAOQQN010000352 DAOQQN010000000
VERSION     DAOQQN010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14262_length_3344_cov_4.921557"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLG24_11410"
     CDS             <1..215
                     /locus_tag="PLG24_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HMZ25545.1"
                     /translation="ISLSKYECPDYSDFDDDEIVWKLDMEYHIGIIVKSMSLQRIKEL
                     LEKYADIIKNEFHAAGPLKPVKHKNS"
     gene            215..673
                     /locus_tag="PLG24_11415"
     CDS             215..673
                     /locus_tag="PLG24_11415"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HMZ25546.1"
                     /translation="MPVVFDLMPFAKQLEVKYVQGKPHFFDKTRRVHLKVTPEEIVRQ
                     SLIIKLHADFGIPWVRMRTEYGIKHNSLQKRIDLLIYDKKGKPWMIIETKAPTVPLSQ
                     LVIDQVAFYNNVIFAPWLMVANGNEAMFASVDFQSKATAEVFEFPLNQWL"
     gene            674..1468
                     /locus_tag="PLG24_11420"
     CDS             674..1468
                     /locus_tag="PLG24_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ25547.1"
                     /translation="MLEFHSDIKTYFNIQYLTSRDHIIPYLSQFMDTCSSIRVLEVGC
                     GEAGVLKAFLERGHDCTGIELEQKRIESARELFRETEHGDRIKFIVKNIYDIDPQRDN
                     LEPFDLIILKDVIEHIPDQEKIIPELKKLSKPGGTLFFAFPPWYMPFGGHQQILRSKI
                     LSKIPWIHLLPGPLYRSLLQMAGEAESTVKELMELKATGISIERFNAIARQHFNIIDT
                     SFWLINPIYEYKFKLRTRKVPGFLTKLPFFRNFYTTAAYFTLRNDK"
     gene            1522..1869
                     /locus_tag="PLG24_11425"
     CDS             1522..1869
                     /locus_tag="PLG24_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HMZ25548.1"
                     /translation="MAIIITDECINCGACEPECPNNAIYEGGVEWRMKDGTTVVGDYT
                     LEDGSVVRYDQLMSPVSDDYYYIVPDKCTECVGFHEEPQCAAVCPVDCCVPDPDRRES
                     DEILLMRKGRLHG"
     gene            complement(1877..2305)
                     /locus_tag="PLG24_11430"
     CDS             complement(1877..2305)
                     /locus_tag="PLG24_11430"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HMZ25549.1"
                     /translation="MEEWSETKANEIISELMLSNDAFSKWLGVRVDLVKESSVEISCN
                     ITETMTNGFHVAHGGIVFSLCDSALAFHSNSWGRKALSIETSINHLSPIRVGDRITAK
                     TSIIKVGKSIGNFLIEAYNQDDKKVAIMKGMVFYLGDRWA"
     gene            complement(2311..>3344)
                     /locus_tag="PLG24_11435"
     CDS             complement(2311..>3344)
                     /locus_tag="PLG24_11435"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HMZ25550.1"
                     /translation="LVATRLAAKGPKILMIGHLDTVFELDSPFQEYTMIDDHLMKGPG
                     VLDMKGGNVVMYLAIKALYELKLLDNLSIKVFLVGDEELSGEPIALSKKDLIEAGKWA
                     DIALGYEDGDGTFDHANISRRGSADWTLKVKGIPAHSSQIFQPEVGAGAIYETSRILH
                     EFYKQLSNEENFTFNPGLISGGNSLTFHEETNKSEAFGKNNIVSAEVIVTGDMRAVSM
                     EQLIRTRRIMQEIVSNSLPNTSATIEFGDDGYPPMTMTEGNIQLLGLFSKVSQDLGYG
                     TVHPVNPRNSGAADISFVAEDVFMAMDAIGLCGTGGHTIEETADISCLPKEAKRTAIL
                     LYRLKDIKF"
BASE COUNT         1011 a          652 c          684 g          997 t
ORIGIN      
        1 ttatttctct gtctaagtac gaatgccctg attattcgga ttttgatgat gatgaaatag
       61 tctggaagct ggatatggaa taccacatcg gaattatagt taagtcaatg agtcttcaaa
      121 gaatcaagga actgctggaa aaatatgctg atataataaa gaatgaattt catgcagcag
      181 gccctttgaa gccagtcaag cataaaaaca gctgatgcct gttgtgtttg atttgatgcc
      241 atttgccaaa caacttgagg tcaaatatgt gcaaggcaaa ccgcatttct tcgacaagac
      301 aaggagagtc catcttaaag tgactcctga agaaattgta agacagagtc ttatcatcaa
      361 actccatgct gattttggta ttccatgggt gagaatgcgg accgaatatg ggataaagca
      421 taattcactt caaaagagga ttgatttgtt gatttatgac aagaagggca agccctggat
      481 gattatagag acgaaagcac ccactgttcc gttaagccaa cttgtaatcg atcaggtagc
      541 attttataat aatgttattt ttgcaccgtg gcttatggtt gccaatggta atgaagccat
      601 gtttgcttca gtcgattttc aatcgaaagc aacagcagaa gtatttgaat ttcctttaaa
      661 tcaatggtta taaatgcttg aatttcattc agatattaag acatatttta atattcaata
      721 cctgacgtca agagatcaca tcattcccta tctttctcaa tttatggata cttgttcttc
      781 gatacgagta ttggaagtag gttgcggtga agccggagta ttgaaagctt ttctcgaaag
      841 agggcacgat tgtacgggaa ttgaactgga gcaaaaaagg atagaatcgg cccgtgagct
      901 atttagagaa acagagcatg gtgacagaat taagtttatt gtcaagaata tatacgatat
      961 tgatccgcaa agagacaatc tggagccttt tgacctgatt atccttaaag atgtaataga
     1021 gcatatcccg gatcaggaga aaataattcc tgaattgaag aaactatcca aaccgggcgg
     1081 gacattattc tttgcatttc caccctggta tatgccattc ggtggtcacc aacagatttt
     1141 aagaagtaaa atcctttcca aaataccctg gatacattta ctaccgggac ctttatacag
     1201 atccttgtta cagatggcag gagaagcgga aagtacagta aaagagctga tggaactgaa
     1261 agccaccggt atcagtattg aacgattcaa cgctattgct cgccaacact tcaatattat
     1321 tgatacatcc ttttggctta tcaatcctat ttatgaatat aagtttaagc ttagaaccag
     1381 aaaggttccc ggttttttga cgaaactacc tttctttaga aatttttata caacagcagc
     1441 ttactttacg cttaggaatg ataagtaaac aaagtgttat ttgtatatgt tgtatctttg
     1501 attaaaattt gactttaata aatggctatt ataataacgg atgaatgtat caattgtggt
     1561 gcatgtgagc cggaatgtcc taacaatgca atttacgaag gcggtgttga atggagaatg
     1621 aaagatggca ccacagttgt tggtgattat acactggaag atgggtcagt ggtaagatat
     1681 gatcaattga tgtctcctgt ttcggacgat tattattata tcgtacctga caagtgtacc
     1741 gaatgtgttg gcttccatga agaaccacaa tgtgcagcag tgtgcccggt ggattgttgt
     1801 gtaccagatc ctgatcgcag ggaatcggac gaaatactat tgatgaggaa gggaagactt
     1861 cacggttaat gtggaatcat gcccaacgat ctccaaggta gaacaccata cccttcatga
     1921 tggccacttt tttatcatcc tgattatatg cctcaatcag gaaattaccg attgatttcc
     1981 caacttttat tatggaggtt ttggcagtta tcctatcacc cactctgatt ggggataaat
     2041 gattgatgga ggtttctata gaaagagctt ttctgcccca ggaattggag tgaaaagcca
     2101 gggcactgtc acatagtgaa aagacaattc cgccatgtgc aacatggaat ccattggtca
     2161 tagtttcagt aatattacat gatatttcga cagaagattc ctttaccaaa tcaacacgca
     2221 ctccaagcca tttgctaaaa gcatcattgc tgagcataag ttcagaaatg atttcattgg
     2281 ctttagtttc tgaccattct tccatggtca ttaaaatttt atgtctttta accggtaaag
     2341 aaggatagca gtgcgtttgg cttccttcgg caggcatgat atatcagctg tctcttctat
     2401 agtatgcccg ccggtaccac acaatccaat ggcatccatc gccatgaaaa catcttctgc
     2461 cacaaaggaa atatcggcag cacctgaatt tctgggattt accggatgca ctgtaccata
     2521 cccaaggtcc tgacttacct tactgaacag gcccagcaac tgaatgttgc cttctgtcat
     2581 agtcatgggc ggatacccgt catcaccaaa ttcgatggtt gctgaggtat taggtaggga
     2641 attgctgact atttcctgca tgattctcct ggttcggatc agctgttcca tggagacagc
     2701 acgcatatcg cctgtgacaa tcacctcggc cgacacaata ttgttttttc caaaagcttc
     2761 agatttattt gtctcttcat ggaatgttaa cgaattacct ccactaatga gacctgggtt
     2821 aaaagtaaag ttttcttcat tactcaattg tttataaaat tcatgaagga ttctcgatgt
     2881 ctcatagatt gcacctgcgc caacttcagg ttgaaaaatt tgtgaactgt gcgcaggtat
     2941 tccttttact ttaagagtcc agtcggctga acctctgcgg gaaatattgg catggtcgaa
     3001 agttccatca ccatcttcat atccaagggc aatatcagcc catttcccgg cttcgatgag
     3061 atctttcttt gataaggcaa taggttcacc gctcaactcc tcatcaccaa ccaaaaatac
     3121 ttttatggat aaattgtcca agagttttaa ctcataaaga gctttaatag ctaaatacat
     3181 aactacatta ccacctttca tgtccaaaac tccggggccc ttcatcaagt gatcgtcgat
     3241 catggtatat tcctgaaagg ggctgtcaag ttcaaaaacg gtatccaggt ggccgatcat
     3301 aagaattttc ggacctttgg cagccaatct tgtagcaacc aaat
//
LOCUS       DAOQQN010000353         3271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_14770_length_3271_cov_5.538246, whole genome shotgun sequence.
ACCESSION   DAOQQN010000353 DAOQQN010000000
VERSION     DAOQQN010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14770_length_3271_cov_5.538246"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLG24_11440"
     CDS             <1..317
                     /locus_tag="PLG24_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25551.1"
                     /translation="VFFFSISTSIPDLRLRPNSRCPYGFLNPLKKQGKDRPLTLDALA
                     HWMLGSYNVTQNRVGIISLCHKVQHMYGPVVLYKIGLRYLTIGHNPFSSKSIVLLLLL
                     RL"
     gene            357..965
                     /locus_tag="PLG24_11445"
     CDS             357..965
                     /locus_tag="PLG24_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25552.1"
                     /translation="MSRKQLLFVCVLLAGMAVFIQCKNDTLPTPKPRSFPRVIYPEKV
                     YRQFDTSFCEFTFEYPAYATIIQDTAFFGEKPVDACWFNIYVPQFQSLIFCTYYPLGK
                     KNTFFKVLNDAYKLAGVHNKKANFIDEIPIDLPGKVYGTIFSIEGPVASTYQFFVTDS
                     TKHFLRGALYFNARTNQDSLAPVAEFMRKDVQHMIKTFKWRD"
     gene            965..2731
                     /locus_tag="PLG24_11450"
     CDS             965..2731
                     /locus_tag="PLG24_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25553.1"
                     /translation="MHQHSKIQAWTAEVIKAFTLRISYILVFSICCSISIYSQVIPIT
                     QNGLVQPLVDRLDMKAGFDPEYHSSHKYLTRGELRRLLLRFDTLAQPEYQDKENIQYL
                     VDENLEGEIYACRKRDSTVLTNFLPLWNSRPILKLFYKTPANFLQADQKDFYVRLNPV
                     LNFGYGRVSGEGAGTTFQNVRGVEMRGGIANRLWFYANLHETQEAYPAYVNDWVKTMG
                     AVPGAGFLKTYQSSLFKGLTGKDYMNAEGIIGFNIIPQIGLQFGHGRNFIGDGYRSLF
                     LSDFAANYLYLKLNTKVWIFDYQNIFAELNATGQTGGDKLIPKKYFANHHLSLNITPW
                     LNVGLFESVVFSRDNHFELQYLNPIIFYRTIEQLVGSPDNAMVGADMKVRLGRSIKLY
                     GQIMLDELIIKNMLKQNGWWGNKYGIQAGIQYYDVAGIDHLDMRLEYNMVRPYTYSER
                     DSASNYSHHFQPLAHPLGANFYEIICQVRYAPTSRWTIEPYCMFAVTGEDHGDINNGS
                     NILKPYTTRTGDFGIGLPNGDRADIVHAGIDVSYMIYHNLFVELSAGYRSKKSVLEAY
                     NTRETWISAGVRMNMAKRKMLF"
     gene            2884..3174
                     /locus_tag="PLG24_11455"
     CDS             2884..3174
                     /locus_tag="PLG24_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HMZ25554.1"
                     /translation="MNYSTFSGFDFEEDVLLKEATTIDQQELIVYNDDYNTFDWVINC
                     FVEILRHTAEQAEQLAYIIHFKGKASVKIGSYYALKPFKEALLDKGLSVVIE"
BASE COUNT          898 a          724 c          727 g          922 t
ORIGIN      
        1 cagttttctt tttctccata tcgacttcta ttccggatct ccgcttacgg ccgaatagta
       61 gatgtccgta tggcttccta aacccgctta aaaaacaagg taaagaccgg cctttgaccc
      121 ttgacgccct tgcccattgg atgttaggct cttacaatgt tactcaaaac agggttggga
      181 tcatttccct gtgccacaag gtccaacata tgtatggtcc tgttgtttta tacaagatag
      241 gactgaggta tctgacgatc ggccataatc cattctcatc caaatccata gttttattac
      301 ttttgttgcg attatgatgt cgatgatcct gtttagatag aatttcacaa aaagatatga
      361 gtcggaaaca actgttattc gtgtgcgtcc tgttggccgg aatggctgta tttatacaat
      421 gcaagaacga tacgctacct acacccaagc cacgctcctt tccgcgggtc atatatccgg
      481 agaaggtgta caggcagttt gacacttctt tttgtgaatt tactttcgaa tacccggcat
      541 atgccacaat cattcaggat acagcatttt ttggtgaaaa accggtcgat gcttgctggt
      601 tcaacatcta tgtgccgcaa tttcagagtc tcatcttttg cacctattat cccctgggta
      661 agaagaatac tttcttcaag gtgcttaatg atgcgtataa attagcagga gtgcacaaca
      721 aaaaagccaa ttttatcgat gagataccca ttgacttgcc gggtaaggta tatggcacca
      781 ttttttcaat tgagggtccg gtagcatcta cttaccagtt ttttgttacc gatagtacga
      841 aacacttcct gcggggggct ctttacttca atgcccgcac caatcaggat tcccttgctc
      901 cggtggcgga attcatgcga aaggatgtgc agcacatgat caagacattc aaatggagag
      961 attgatgcat caacatagta aaattcaggc atggactgcc gaagtaatta aagcctttac
     1021 gctgcggata tcttatatct tggtttttag catttgctgt tctataagta tatactcaca
     1081 ggttattccc attacacaaa atggccttgt gcagcccctg gtcgatcgtc tggatatgaa
     1141 ggccggtttt gatccggagt atcattcatc ccataaatac ctgacgcggg gtgagctgag
     1201 gcgtctgttg ttgcgctttg atacgttggc gcaaccggaa tatcaggaca aggagaatat
     1261 ccaatatctc gtggatgaaa atctggaagg agaaatatat gcatgccgca aaagagattc
     1321 gactgtcttg accaactttc tgccactgtg gaattccaga ccgattctga agctcttcta
     1381 caagacaccg gcaaatttcc tccaggcgga ccaaaaggat ttttatgtga gactcaatcc
     1441 ggtgcttaac tttggctatg gcagggtttc gggagaaggc gccggcacaa cttttcaaaa
     1501 tgtacgagga gtagagatgc ggggaggaat agccaaccga ctgtggtttt atgccaatct
     1561 tcatgagacc caggaagctt atccggctta tgtcaatgac tgggtgaaaa ctatgggtgc
     1621 cgtgccggga gccggcttcc tgaagaccta tcaatcctct ctcttcaaag gtctcacagg
     1681 taaagactac atgaatgcgg aggggatcat cggctttaat atcatcccgc aaattggact
     1741 ccaatttggc cacggacgca actttatcgg tgatgggtac cgttcgcttt ttctttccga
     1801 ctttgccgcc aactaccttt atctgaaact gaataccaag gtgtggatct ttgactatca
     1861 gaacatcttt gccgaattga atgccaccgg acagaccgga ggcgacaagc tcatccccaa
     1921 gaaatacttc gccaatcacc atctgagcct caacatcaca ccatggctca atgtcggtct
     1981 ctttgaaagt gtcgtattca gcagggacaa tcatttcgag cttcaatatc tcaatccgat
     2041 catcttctac cgtacaatcg aacaattagt gggcagccct gacaacgcca tggtcggtgc
     2101 cgatatgaag gtccggttag gcaggtcgat caagttgtac ggtcagataa tgctggacga
     2161 gctaatcatc aagaatatgc tgaagcaaaa tggctggtgg ggcaacaaat acggcataca
     2221 ggccggcata cagtattatg atgtcgccgg catcgaccac cttgatatga ggctggaata
     2281 caatatggtc aggccatata cttattcgga acgcgacagt gcttctaatt attcacacca
     2341 tttccaacca ctggcgcatc ccttgggagc caatttttac gaaatcatct gtcaagtgcg
     2401 ttatgcgcca acgtcacgct ggaccatcga accttactgt atgtttgcag ttaccgggga
     2461 agaccatgga gatatcaata atgggagcaa tattcttaaa ccatacacga cacgaaccgg
     2521 agatttcggg atagggctgc caaacggcga tcgggcagat atcgtccacg caggcattga
     2581 tgtgtcgtat atgatttacc acaatctgtt tgtcgagttg tcagccggct ataggagcaa
     2641 aaagagtgtg ttggaggctt ataatacaag ggaaacatgg atatccgcag gtgtccgcat
     2701 gaatatggca aagcgtaaga tgttgttctg aacaatatga gtctccaata cattaggata
     2761 aaattcattt gttatttaag ttaaaatttt attaactttg gtgatataaa cattaatttt
     2821 gcacctttga atatttccga taacggaaga atcaataata atcctgacaa tttttgacac
     2881 tatatgaact attccacatt ttcaggcttc gattttgaag aagatgtcct gttgaaggaa
     2941 gcaacaacta ttgaccaaca ggaactgata gtctataatg acgattacaa tacatttgac
     3001 tgggttatca actgttttgt ggagatacta cgtcataccg cagaacaggc agagcaactc
     3061 gcttatatca tacacttcaa gggtaaggca tcggtcaaga tcggctctta ttatgccctc
     3121 aagccattca aggaagctct tttggacaaa ggcctttctg tcgtaataga ataaaatccg
     3181 ggttccggac gattttcctt gggcgtttct gaaaattttt tcgtttccga ctcaactttc
     3241 aggcatccgt ctgttgtggc tcccgatggt t
//
LOCUS       DAOQQN010000354         3271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_14772_length_3271_cov_5.106032, whole genome shotgun sequence.
ACCESSION   DAOQQN010000354 DAOQQN010000000
VERSION     DAOQQN010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14772_length_3271_cov_5.106032"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(407..2914)
                     /locus_tag="PLG24_11460"
     CDS             complement(407..2914)
                     /locus_tag="PLG24_11460"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HMZ25555.1"
                     /translation="MQKLIPEYLKGLDATEVEDSRQRFGFNSLRSGSEAAWYDLLVDI
                     FKEPMLILLVAVSIIYLIIGDWGEAAFMFVAIVAVSAISFYQDNRSRKALQELEKLNE
                     PLSTVIRNSEILKIPTREIVVGDLCVVEEGKMVNADGRIVHSNDFSVNEASLTGESMP
                     VFKRADTADSSVYSGTIIVSGLAVFEVEKIGAETRIGRIGTSMKDIGDEASPLQLQIR
                     TFVKNMAIWGIIVFVMICIYRFYQTGDIIESLLSGLTLAMSVLPEEIPVAFTTFMALG
                     AWKLINEGIIVKRSSVVETLGSTTVICTDKTGTITQNSMHLHMMYDFSSGQTYLAHEF
                     SDAALTDLISYAMWASEPVPFDPMEKELHRIYGETANEDLRPQFHMAHEYPLGGIPPM
                     MTHIFENDNGNRIVAAKGAPEAILEVSELDMEQLEDMRAMVRKFSGQGFRVLGVGASD
                     FAASGFPPAQQDLVFRFLGLLIFQDPPKPGIRNVFSKIYQAGIKVKMITGDNTDTAVA
                     IAQQAGIINDAKPVEGQFISSLGEEELRSIVRDTVLFTRMFPDAKLEVVKTLKADGEV
                     VAMLGDGVNDGPALKAAHIGVAMGHKGTEIAKSAASLVITDDDLDKLITGIAAGRRIY
                     TNLKKAVQYIISIHIPIILTVSLPLFLGWVYPAIFTPVHVIFLELIMGPTCSIVYENE
                     PIERHAMSGPPRRMADTFLSWRELAVSILQGLMITGGVLTAYQWMAQNGASEEKTRAM
                     VFATLVLANIFLSFANRSFYYSLFECFMRKNLLFPVITLATLIFLLMILYLPAVSGFF
                     HVTGLTVKEISISVCIAFGSILWFEIYKIGRRVSKKY"
     gene            complement(2997..>3271)
                     /locus_tag="PLG24_11465"
     CDS             complement(2997..>3271)
                     /locus_tag="PLG24_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25556.1"
                     /translation="FIRHSESLEAYQSFLKTEYREDTLSGAHLYGDDFEETIQKYIEE
                     HEIDILVMVTYQDNIWARFFNPSKTRAMSYQTRVPLLAIPADFGKK"
BASE COUNT          957 a          822 c          627 g          865 t
ORIGIN      
        1 gatttttcgc tacagaagtt tttgaaatat tacaaaaagg ggattaacat agaacgttcc
       61 cggattacgc tgcctgatat ttacactgac gaagcatagc agtagtaata ccgaaggaac
      121 attcgtaaaa gaccaatttc attggcctga acgactgtaa tatcggcatc cccgagcccc
      181 ggattgtatc acttcctgga cagagcagag ctctggtcgt tgtactctcg ggacaagcat
      241 acctaaaact atcggattat tttatcattc atccgggtat aagatcaata catctaatgg
      301 agaaatgatt cacccctata aaaaacgatt ttcaaaaact acgcattcca agctaaaaga
      361 ctcatgcaca ggtaaacaat ttttttcctt cacccacaac attctttcaa tacttcttac
      421 tgactcttct gccaatctta taaatttcaa accacaatat cgaaccaaag gcaatgcata
      481 ctgatatact gatctcctta accgttaagc ctgtcacgtg aaaaaaccct gaaaccgcag
      541 gaagataaag aatcatgaga aggaagatca gggttgcaag cgtgatcaca gggaatagga
      601 ggttcttcct cataaaacat tcaaacagac tataataaaa tgatcgatta gcaaagctta
      661 gaaagatatt tgctaatacc aaagtggcaa acaccatagc cctcgttttc tcctctgaag
      721 caccattttg agccatccat tgataagccg tcagaacacc tcctgtaatc atcaaacctt
      781 gcaaaatact gacggccagc tcacgccaac tcaggaaggt atctgccatc ctgcgaggcg
      841 gcccggacat agcatgtcgt tctatcggtt cattctcata cacgatggaa caggtaggtc
      901 ccataatcaa ctccaaaaaa atgacatgta caggggtaaa gatcgccgga taaacccaac
      961 ccaggaacaa aggcaaggat acagtcaaga taatcggtat gtggatggag ataatgtatt
     1021 gaaccgcttt cttgagattg gtatagatac ggcgtccggc ggcaatacct gtgatgagct
     1081 tatccaggtc atcgtccgtg ataaccaaag acgctgcaga cttggctatt tcggtacctt
     1141 tatggcccat ggccacaccg atatgcgcag ccttcaacgc aggtccgtca ttgacaccat
     1201 cccccaacat agccactacc tccccatcag cctttaaggt cttgactact tcaagtttgg
     1261 catcgggaaa cattctggta aacaatactg tgtcacgtac tatcgacctc aactcttcct
     1321 ctcccaggga agaaatgaat tggccctcaa caggttttgc atcgtttatg ataccggctt
     1381 gttgagcaat agccacagcc gtatcggtat tatcaccggt aatcatttta acctttatgc
     1441 ctgcctgata gatcttactg aaaacgttcc ggataccggg cttgggagga tcctgaaata
     1501 tcaaaaggcc gagaaatcga aataccagat cctgttgtgc gggaggaaat cccgacgctg
     1561 caaaatcaga tgcaccgacg cccaagaccc gaaaaccttg tccactaaac ttcctaacca
     1621 tcgcccgcat gtcttcgagc tgctccatat caagttcgga tacttcaaga atggcctccg
     1681 gcgctccttt tgcggcaact attctattgc cattatcatt ttcgaagata tgcgtcatca
     1741 tgggaggtat tccgcccaat ggatattcgt gcgccatatg aaattgtggc cttaaatctt
     1801 cattggcggt ctctccatag atcctgtgta attccttctc catcggatca aaaggcacag
     1861 gttcactggc ccacatggca taagaaatca ggtcagtcaa tgcggcatcc gaaaactcat
     1921 gggccaaata agtctgaccg gatgaaaaat cgtacatcat atgcagatgc attgaatttt
     1981 gcgtgatggt tcctgtctta tcggtacaaa tcacggttgt gcttcccaat gtttccacga
     2041 cactagaccg tttgacgatg atgccctcat tgattagttt ccaggcgccc agggccataa
     2101 atgtagtgaa tgccaccgga atctcttccg gcaatactga catggcaagc gtcagaccgc
     2161 taagtaagct ttctattatg tcaccggtct gataaaaccg ataaatacaa atcatcacaa
     2221 aaacaatgat gccccaaatg gccatattct tcacgaaggt gcggatctgc aactgaagtg
     2281 gtgaagcctc atcccctata tccttcatcg aagtaccaat cctgcctatc cgtgtttccg
     2341 cgccaatttt ctccacttca aataccgcaa gtccggaaac aataatagtg ccactatata
     2401 cgctggaatc agccgtgtct gctcgcttga ataccggcat actctcaccg gtaagcgatg
     2461 cttcattgac tgaaaaatca ttactatgaa caatgcgccc atcggcattg accatcttac
     2521 cttcttcaac gacacacagg tcacctacaa caatctcgcg ggtaggaatc ttcaatatct
     2581 ctgagtttct aattaccgta ctcaggggtt cattcaattt ttcaagttcc tgtagcgctt
     2641 ttctactcct gttatcctga taaaacgaaa tagctgatac agccactatg gccacaaaca
     2701 taaatgcagc ttcaccccaa tcaccaataa tcaggtaaat aattgaaacc gcaaccaaca
     2761 aaatcagcat cggctctttg aagatatcca ctaaaagatc ataccaggca gcttcgctgc
     2821 cactcctgag gctattgaat ccaaatcttt ggcgggagtc ctcaacttcc gttgcgtcta
     2881 gacctttgag gtattccggg ataagtttct gcatcctact ttcattctgc tatgactaca
     2941 agctacagcc tgaaagcgac tgtatgctgt ccgatgattt ttcattatcc ggtttactac
     3001 ttttttccga aatctgccgg aatagcaagc aggggaaccc ttgtctgata gctcatagca
     3061 cgcgtcttac tcgggttgaa gaatcttgcc catatattat cctgataagt caccatgacc
     3121 aagatatcaa tttcatgctc ttcaatatat ttctggatag tttcttcaaa atcatcacca
     3181 tacagatggg cgccggataa ggtatcctcc ctgtattcag tcttcaggaa agactggtat
     3241 gcttcaaggc tctcagaatg tctgataaat t
//
LOCUS       DAOQQN010000355         3258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_14861_length_3258_cov_5.939744, whole genome shotgun sequence.
ACCESSION   DAOQQN010000355 DAOQQN010000000
VERSION     DAOQQN010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14861_length_3258_cov_5.939744"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..623
                     /locus_tag="PLG24_11470"
     CDS             108..623
                     /locus_tag="PLG24_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25557.1"
                     /translation="MSLHTSLSRFFLKRFLRERKDTSSTFKLAENNYQSLLLVFQNQT
                     DVARIVQSIKTIAGRTFSRIDYIIFTDNKSENAPEKGILSTRLVGMQGKFSAKGTQPV
                     HSSAYDIIVDMCDVTELNLIHYLLKLSPQLFVRFNKEKGIFYNFIVPTGQKDLLARFE
                     QFYRIFQTLSK"
     gene            620..1525
                     /gene="dapA"
                     /locus_tag="PLG24_11475"
     CDS             620..1525
                     /gene="dapA"
                     /locus_tag="PLG24_11475"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HMZ25558.1"
                     /translation="MNNKARFQGTGVAVVTPFKSGQVDYEALTNVLNHVVDGGVDMIV
                     CLGTTGEATALSDHEQQEILAHTIEVVAGRKPIVYGPFGSNNTAALTQRIVSTDLSGV
                     DAIMSSSPAYVKPTQEGIYQHYMRIADSSAKPVIIYNVPSRTAAKVSHETILRLAESH
                     DNFLGVKEATADFFALSKLIKHRPDNFLVWSGDDYTAFPTMALGADGGISVIANAFPR
                     EFSEMIRACLKGDFARGAELHMQMLDLHPILYAEGNPTGIKGAMEDLGLCSKEVRLPL
                     TPLSEAANRQLIHEIKKAGLAGVKE"
     gene            1556..2017
                     /locus_tag="PLG24_11480"
     CDS             1556..2017
                     /locus_tag="PLG24_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ25559.1"
                     /translation="MVTIEEKTEISTSEKDFIQRIWNSEFPAVSKLDENKELEFYLKK
                     TTDHRYYFLKVDGKLAGWGFKFKRYSGTWFALALEREYQGKGLGSKLFNEIKKNETEI
                     NCWAIDENIHNKADGSLYRSPFDFLKANGMTPFENIRGHDGKLTAIKFKSS"
     gene            complement(2057..2995)
                     /locus_tag="PLG24_11485"
     CDS             complement(2057..2995)
                     /locus_tag="PLG24_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HMZ25560.1"
                     /translation="MQIIQAEGDKEDVVTIKGSRFSYEFRPKWGGILNQWEVLTDCNW
                     RAVINAFDSAEDFKSNYGNKGFPSCKLSPYACRIRDSRYLFEGKSYRIGKFGYDRHNI
                     HGLLFDSEFSIDNIIESDDKVNIVLVTTYRGEDSGFPFLYDLKLDYQVDDSGFICIKT
                     TVKNVGNSGMPVVDGWHPYFSVSSRIDDVSLCILADEVLQFDENLIPTGEKLVFSDFN
                     SPGLIGGMQLDNCFPLNGRTGMVCSLSYEELKLEISAWKHYNYLQLFIPPDRKSIAIE
                     LLSGAPDAFNNGMGLTVLQPGESRIFECSYHLKLSQ"
BASE COUNT         1052 a          722 c          611 g          873 t
ORIGIN      
        1 caaggaaggt aataagaaca tggtccggaa gcggattgag aagtaccgca acatgggtaa
       61 gtttcatatt aaggaaggtt gattatttcg attaatatct acagcacttg agcctccaca
      121 cgtcgctttc ccgttttttc ctgaaaagat tccttaggga aagaaaagat acatcatcaa
      181 cattcaagct cgctgaaaat aattatcaat ccctgttgct tgtattccaa aatcaaacgg
      241 atgtcgcccg aattgtacaa tccataaaaa caattgccgg caggacgttt tcccggattg
      301 attacatcat atttactgac aataagtctg aaaatgcccc ggagaaagga atcctaagta
      361 caagacttgt aggcatgcag ggcaaattct ctgccaaggg cacacaaccg gtgcattcat
      421 cagcctatga tatcattgtc gatatgtgtg atgtcactga actgaacctg attcactatt
      481 tgctgaaatt atcgcctcaa ctttttgtaa gatttaataa agagaaagga attttttata
      541 actttatcgt tccaaccgga caaaaagacc ttctggcccg gtttgaacaa ttttacagga
      601 tatttcaaac cttatcaaag tgaataacaa agcacgtttc caaggtacag gcgtagcggt
      661 tgtaacccct ttcaagtcag gacaagtcga ttacgaagcc ctcaccaatg tgttgaatca
      721 tgtggtcgat ggcggcgtgg atatgatcgt gtgtttgggc acaaccggag aagccacggc
      781 tctcagtgat catgaacaac aagagatcct tgcccacact attgaagtgg tcgccggaag
      841 aaaacctatc gtatatgggc cttttggaag caacaataca gctgccctga cacaacgcat
      901 cgtttccacg gatctgtccg gagtcgatgc aattatgtcc agcagtcctg cttacgtcaa
      961 gccgacccag gaaggcatct atcagcatta tatgcgcata gccgactcaa gtgccaaacc
     1021 tgttatcata tacaatgtac caagccgtac cgctgcaaag gtatcacacg aaaccatatt
     1081 acgtctggca gaatcacacg acaatttttt aggagttaag gaagcaaccg ctgatttttt
     1141 tgccttgagc aaattgataa aacaccgacc tgacaacttt ttagtctggt caggcgacga
     1201 ttatacagca ttccccacca tggcactggg agccgacgga ggtatatcgg ttatcgcaaa
     1261 tgcctttcca cgggaatttt ccgaaatgat cagagcatgc ttgaaaggag attttgccag
     1321 aggcgctgaa ctacacatgc aaatgctcga cttacatccg attctttatg cagaaggcaa
     1381 cccgacagga ataaaaggcg ccatggaaga ccttggactt tgctccaaag aagtaagact
     1441 ccctctgaca ccccttagtg aagcagctaa ccgacagtta atacatgaaa tcaagaaagc
     1501 aggactagcc ggtgtaaaag aataaatatt attttttaag gtaattcggt gtctcatggt
     1561 aacaatcgaa gaaaaaaccg aaatatctac ctcggaaaaa gactttatcc aaagaatctg
     1621 gaacagcgaa tttccggcgg ttagtaaatt agacgaaaac aaagaactcg aattctacct
     1681 gaagaaaacc accgaccatc gatattattt tctgaaagtg gatgggaaac tggccggatg
     1741 gggtttcaaa ttcaaacgtt actccggaac ctggtttgca ctggccttag aaagagaata
     1801 tcagggcaag ggactaggct caaaactatt taatgaaatc aaaaaaaatg aaactgaaat
     1861 taattgttgg gctatagatg aaaacataca caataaagct gacggatcac tatatcgctc
     1921 gcccttcgac ttcttaaaag ccaatggaat gacgccgttt gagaatattc gtggtcatga
     1981 tggaaagcta actgcaatca agtttaaatc atcctgactt ttgctaataa gaataaaaat
     2041 tggataaaat tctggactac tgcgataatt tgagatggta agaacattca aatatcctcg
     2101 attctcccgg ttgaagaact gtcaaaccca ttccattatt gaatgcatcc ggagccccac
     2161 tcagcaactc aatggctata ctttttctgt cgggaggaat gaataattgc aggtaattat
     2221 aatgtttcca cgcagatatc tccaacttta actcctcata cgaaaggcta cacaccatgc
     2281 cggtcctacc attcaatgga aaacaattat ccaactgcat accgccaatc aatccgggtg
     2341 aattaaaatc agagaaaacc aacttttctc ccgtaggtat caggttttca tcaaattgca
     2401 atacctcatc tgctaagata caaagactca catcatcaat ccgggatgaa acagaaaaat
     2461 aaggatgcca gccatcgacc acaggcatac cagaatttcc aacattcttt actgttgtct
     2521 taatacagat gaatcccgaa tcatccacct ggtaatccaa cttcaaatca tatagaaaag
     2581 ggaaaccact atcttcacct ctgtatgtgg tgacaagtac aatattcacc ttgtcatccg
     2641 actcaatgat attatcgatc gaaaattcgc tgtcaaataa taagccatga atattatgcc
     2701 tgtcatagcc aaacttccca atccgataac tcttgccctc aaataagtaa cgactatccc
     2761 tgatgcgaca ggcataaggg ctcaacttgc atgatgggaa gcccttgttg ccataattgc
     2821 tcttaaaatc ttcagcggaa tcaaatgcat ttatgacagc cctccaattg cagtcggtca
     2881 aaacttccca ttgattcagg ataccacccc atttaggcct gaactcataa ctgaatctac
     2941 tcccttttat agttactaca tcttccttat ctccttctgc ttgtatgatt tgcatctgaa
     3001 agtttattac ggacattcct tcaccccaag atataaaaaa atacgcaatc cggagtggaa
     3061 tgcgtattat aaaaaatact acctcttatt caataaaaaa ccaacttttc atttctgtaa
     3121 gttggctcgt tcttgaagcc ctcaaataag tgtgtgttca attatcgttt atttctttgt
     3181 aagagcttca acaaactgat ataacaaaag taattgattt aagcctgtcc tctaatggct
     3241 tttttatcat ttgagtgt
//
LOCUS       DAOQQN010000356         3249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_14916_length_3249_cov_5.885097, whole genome shotgun sequence.
ACCESSION   DAOQQN010000356 DAOQQN010000000
VERSION     DAOQQN010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14916_length_3249_cov_5.885097"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLG24_11490"
     CDS             <1..343
                     /locus_tag="PLG24_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the phosphorylation of glucose using
                     polyphosphate or ATP as the phosphoryl donor; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HMZ25561.1"
                     /translation="GIGSALFYNGVLVPNTEFGHLKFHKSIAEKYTSNATRKEKDLSW
                     DEFGGRLNEFLKHLEFIFSPNLFILGGGLSKKFDLYKEFFALETTIVPAKFQNEAGVI
                     GAALYAHRMNF"
     gene            350..1228
                     /locus_tag="PLG24_11495"
     CDS             350..1228
                     /locus_tag="PLG24_11495"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HMZ25562.1"
                     /translation="MAEKSKHPVRKKPGGFDKFRKVEAVEDKDDLRDAKRRPRKDKAE
                     KRPNPVDEQNTTPRLNKYVANSGVCSRRAADTLIANGEVKVNGSPVLEPGYRVQPHDK
                     VTYQGKHIMPVEQKIYILLNKPRDVITTLSDEKGRKSIHDLIKGKVSTHVFPVGRLDR
                     DTTGLLLITNDGDLTKRLSHPSYEVKKIYHATLNKPLSEADLAKIRAGITLEDGFIKV
                     DGISYVRDAGPHEVGITLHSGRNRIVRRIFEFFGYDVVKLDRVYYAGLTKKDLPRGRM
                     RFLTKEEVIRLKHFKV"
     gene            complement(1319..2026)
                     /locus_tag="PLG24_11500"
     CDS             complement(1319..2026)
                     /locus_tag="PLG24_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25563.1"
                     /translation="MPEKIPCDVYQTMKVDQLLETPREVRDSVWRTEFFNAVTTACFK
                     CGEPQMSHGPDGFPYFELHLPEPEESFESYCIHNLMDYLLEIGYGVVLNPKGEDADWV
                     FSYGDIVNLSLNQEIYSVPDHDPLPEYYATDEEEKVMIFQPSEEYLPGIVKKMLHSFL
                     TYLHVKEPSMLAFIRSGGTENSGVTQLSFNIFPEDYGSEDEYLIRLKQLSWFLPRHYI
                     ITGVSRSNPITQYFEPL"
     gene            2437..>3249
                     /locus_tag="PLG24_11505"
     CDS             2437..>3249
                     /locus_tag="PLG24_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ25564.1"
                     /translation="MEDNGLLYIEDNIKTLDWKEHIRMRPGMYIGKLGDGQSNDDGIY
                     ILIKEIIDNAMDEYFMGHGKEIVIRISGDRVMVRDYGRGIPLGKVIDCVARINTGGKY
                     DSKAFKKSVGLNGVGTKAVNALSESFTVQSFRDGRTKKADFSKGELVKDYPEGKTDEK
                     NGTIIEFTPDNDIFRNFHFREDIVEPMLWNYAYLNSGLKINFNGKTFVSKNGLADMIA
                     GRTNPEELIYEPIHLTGDDIEIVLTHGHQYGEQYYSFVNGQNTTQGGTHLNAF"
BASE COUNT          948 a          665 c          720 g          916 t
ORIGIN      
        1 aggcatcgga agtgcactgt tttacaatgg agttcttgtg ccgaatactg aatttggaca
       61 ccttaagttt cataaatcca ttgccgaaaa atatacctcc aatgcaacca gaaaagagaa
      121 ggatttgagt tgggatgaat ttggaggcag gctcaacgaa ttcctgaagc atcttgagtt
      181 tatcttttct cccaatttat ttattctcgg tggtggactt agtaagaaat ttgatctcta
      241 caaagagttt tttgctcttg aaactactat tgtaccagcc aaattccaga atgaagccgg
      301 tgtgattggt gctgctcttt atgctcacag aatgaatttt tgattgtcaa tggcggaaaa
      361 aagtaaacat cctgttcgaa aaaaaccagg cggatttgac aaatttcgca aagtagaggc
      421 tgttgaggat aaagatgatc tccgggatgc gaaacgccgg ccacgcaagg ataaagccga
      481 aaaaagaccg aatccggttg atgagcaaaa cactacgcca cggctgaata aatatgtagc
      541 aaactcgggt gtttgcagta gacgtgcagc cgatactttg atagccaatg gtgaagttaa
      601 ggtcaatggt tctccggtcc ttgaacccgg ctatcgggta caaccacatg ataaggtgac
      661 ctaccagggc aagcacatca tgcctgtaga gcaaaaaata tatatactgc tcaataaacc
      721 gagggatgtc atcactacac tttctgacga aaaaggcaga aagagcatcc atgacctgat
      781 caaaggcaag gtttccactc atgtatttcc ggtgggcagg ctcgacaggg atacaaccgg
      841 gctgttgctg attaccaatg atggggacct gactaaaagg ctttcacatc cttcctacga
      901 agtcaaaaag atttatcatg ccacactcaa taaacccttg tctgaagctg atctcgcaaa
      961 gattagggcg ggaataacac tggaagatgg ctttattaag gtggatggca tttcatatgt
     1021 cagggatgcc ggcccccacg aggttggaat taccttgcat tcaggccgca accgaatcgt
     1081 ccgccggatt tttgaattct tcggttatga tgtagtcaaa ctggatagag tctattatgc
     1141 aggattgacc aaaaaggatc ttcctcgtgg aaggatgcgg tttttgacaa aggaggaagt
     1201 cattaggctg aagcatttta aggtttaatt acttgtttga aataggtata aagcatgagt
     1261 cagtcaggtg tttactacct gcatcaatcc gtgtagtgac cttggctacg gtactctctt
     1321 atagaggttc aaaatactga gtgatcggat tactcctgga gacacccgtg attatataat
     1381 gtcgtggaag aaaccaactc aattgtttaa gtcggatgag gtattcatct tcacttccat
     1441 aatcctcagg aaatatattg aaagatagtt gtgttacccc tgaattttca gtcccgccgg
     1501 atctgataaa tgcaagcatt gacggctcct tcacatgcag gtaggtcagg aaggaatgca
     1561 acattttttt tacgataccg ggaagatatt cctctgatgg ctggaatatc attacttttt
     1621 cctcttcgtc tgttgcataa tattccggta gaggatcgtg gtccggtacg gaataaatct
     1681 cttgatttag gctcagattg acaatatctc cataggaaaa tacccagtcg gcatcttccc
     1741 ccttgggatt aaggaccacg ccataaccga tttccaacag atagtccatc aaattgtgga
     1801 tacagtaact ttcaaagctc tcttccggtt caggtaaatg taactcaaag taagggaagc
     1861 catccggccc gtgtgacatc tgtggttctc cacatttaaa acatgccgtg gtcacagcat
     1921 tgaaaaattc agtcctccat actgagtcgc gaacctccct gggcgtctcc aacagttgat
     1981 ccaccttcat ggtctgatat acatcacagg ggattttttc cggcataata tgagaatttg
     2041 tggcgtgaag ataggaaaat tgaatgcgat tactgtcaat ttgcctgctt aaattcactt
     2101 attcagagaa ctccttttcc gtgaattttc gtcatagcat ggtcaccaca ttccttcacc
     2161 tcattatcct aagccctgtc gatatttgat taaccttacg ctccctccca tacaccatcg
     2221 attcccggga aacatcggcc cttcaggcta tttgttctat tcttggattc attttgctac
     2281 catagctcca ctcgtacata aattttcacc ctggtttatt ttcaagaatt ggatcggcca
     2341 aaggaggtct atccggcaat tcggcttact tacaccaaat cctcattgta taaggctttt
     2401 atttaattta taaaaaaata tatgtaattt tgcagcatgg aagacaatgg tttactctac
     2461 attgaggaca atatcaagac actcgattgg aaggagcata tacgtatgcg cccgggtatg
     2521 tatattggaa agcttggtga cggacagtct aatgatgatg gtatttatat ccttattaag
     2581 gagattattg ataatgccat ggatgaatac ttcatggggc atggtaagga aattgtcatc
     2641 cgtatatccg gtgatcgggt gatggtccgc gattatggca ggggtatacc tttgggaaaa
     2701 gtcatagact gtgtcgcccg aatcaacaca ggaggaaaat atgattccaa ggcttttaag
     2761 aagtccgttg gattaaatgg tgtcggaacc aaggccgtca atgcattaag cgagtctttt
     2821 accgtgcagt cgttcagaga tggtcgtacc aaaaaggcag atttttcaaa aggtgagctg
     2881 gttaaggatt atcctgaagg aaagaccgac gaaaaaaacg gcactatcat cgagtttaca
     2941 cccgataatg atattttcag gaattttcac tttagggagg acattgtcga acccatgttg
     3001 tggaattatg cctatctcaa cagtggtcta aagatcaatt tcaacggaaa gacatttgtc
     3061 tccaaaaacg gactggccga catgattgcc gggagaacca atccggagga attgatttac
     3121 gaaccaattc atcttaccgg tgatgacatt gaaatcgttt tgacccatgg tcatcagtat
     3181 ggtgagcaat attattcttt cgtcaacggt cagaatacaa cacagggagg tactcatctc
     3241 aatgcattc
//
LOCUS       DAOQQN010000357         3183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_15460_length_3183_cov_5.144501, whole genome shotgun sequence.
ACCESSION   DAOQQN010000357 DAOQQN010000000
VERSION     DAOQQN010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15460_length_3183_cov_5.144501"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PLG24_11510"
     CDS             <1..756
                     /locus_tag="PLG24_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HMZ25565.1"
                     /translation="IAMGMIAEEGRLAILTDILGDEDALGDMDFKVTGTSKGICGCQM
                     DIKIDGLPYEQLEKALAQAKAGRLHILGKMNETISKPNPDLKPHAPRMIEIIIDKSFI
                     GAVIGPGGSIIQDLQEKTGTVITIEEKEGKGFVNITSSDKAGIDAAVARIRQISFQPE
                     IGDVYDAVVKEILSFGAVVDFSGKQGLLHISEISHQRIANVEDVLKVGDHISIKLLDI
                     DRKTGKFKLSAKVLQPKPERPPHQDRGDNRNRR"
     gene            936..>3183
                     /locus_tag="PLG24_11515"
     CDS             936..>3183
                     /locus_tag="PLG24_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HMZ25566.1"
                     /translation="MTEDNKKEIHSIVPIGGMYQNYFLDYASYVILERAVPMIEDGCK
                     PVQRRILHSMKEMDDGRYNKVANIIGHTMQYHPHGDASIGDALVGMGQKDLLIDCQGN
                     WGDPRTGDSAAAPRYIEARLTKFALDVIFNPDTTPWQISYDGRKNEPVHLPVKFPLLL
                     SQGTEGIAVGLATKILPHNFNELLDACVAYLRNEPFVLYPDFLTGGFADVSNYNSGNR
                     GGRVLSRAKIEIPNRNMLEITELPFGVTTDRLIESILKANDKGQIKIKKITDNTARDV
                     NISIELMPGISPDQTMDALYRFSDCEVSISTMCCIIHDDKPVFIGVEEVLKANVDQTK
                     QLLKQELQIKLDELTEKWFFTSLEKIFIEQRIYRDIEEAESFEQVISIIRAGLEKYVS
                     TPTHPEPTIKLLAKLLRDINEDDIIRLTEIKIKKISKYSSFETDQALKTLLEEISKVK
                     HDLKHLVEFTIQWFTYLKDKYGKGKERKTILTQFEAIEATAVVQNNSKLYVNREEGFV
                     GFGLKKDEFVSDCSDIDDIIVFTAQGKMMVTRLSEKTFVGTNIIHVAVWKKNDERTTY
                     NMIYSDGPGGKAYAKRFNVTAITRDKEYDLTKGAAKPRLHYFSANENGEAEIVKVTLS
                     QTSKAKIKVFDFDFSTQLIKGRDSQGNIVTKYPVRKITLEEKGGSTLGAQVLYFDEET
                     GKLNVDDKGKKIGSIDNGDKLVIFYSNGSLELVPVDLSLKLNAREILNIAIFNPEMIV
                     NVVHFDGHK"
BASE COUNT         1008 a          597 c          740 g          838 t
ORIGIN      
        1 attgcaatgg gaatgatagc agaagaaggc cgacttgcca tactaacaga tattctgggt
       61 gatgaagatg cattaggtga tatggacttc aaggtgacag gaacctccaa aggtatctgt
      121 ggttgtcaga tggatatcaa aattgacgga cttccatacg agcagcttga aaaggcgctg
      181 gcacaggcaa aggccggaag attgcacata ctgggaaaaa tgaacgagac cattagcaaa
      241 cctaatccgg atcttaagcc acatgcccct cgaatgattg agatcatcat cgacaagagc
      301 tttatcggtg ctgttatcgg tcctggagga agcatcattc aggacctcca agagaagaca
      361 ggcacagtca tcaccataga agagaaagaa ggtaagggat ttgtcaatat cacaagctca
      421 gataaggccg gtatcgatgc tgcagtggca agaatccgcc agattagttt tcaaccggaa
      481 attggtgatg tgtacgatgc tgtagtcaag gaaatcctat cttttggcgc tgttgtcgat
      541 ttcagcggaa aacaaggttt gttgcacatc agtgaaatat cccaccagcg aatagcaaat
      601 gtcgaggatg tattgaaagt aggtgatcac ataagtatta agctgctgga tatcgatcgg
      661 aaaaccggta aattcaaatt gtcggccaaa gtattgcagc ccaagcctga acgacctcct
      721 catcaagatc ggggcgataa tagaaacaga agataaattg gaaataataa ggctgtcttt
      781 caggcagcct tttttattgc ttttttttgt ggttatagcc ttgtcaataa tgctgtacat
      841 tgatgcctga aaagcatatg ccatttttat gataaaataa aatatatata atatgttctt
      901 atctttgtgg gaatatatta taaagactag gttctatgac tgaagataat aaaaaggaga
      961 tacactcaat tgttcccatt ggtggaatgt accagaatta ctttcttgac tatgcttcct
     1021 atgttatcct tgagcgagct gtacctatga ttgaggacgg ctgcaagccc gttcagcgta
     1081 gaattctgca tagtatgaag gagatggatg acggccgtta caataaagtg gccaatatca
     1141 ttggacatac catgcaatat catccacatg gtgatgcttc catcggtgat gcccttgtag
     1201 gcatgggcca gaaagattta ttgatcgatt gtcagggcaa ttggggtgat ccgaggactg
     1261 gcgattcggc agcggcaccc cgatatattg aagcaagatt gacaaaattt gccctcgatg
     1321 tcatcttcaa tcctgacacg accccctggc agatttcata tgatggaagg aagaacgaac
     1381 cggtacattt gcccgtcaaa tttccactct tgctcagcca gggtaccgaa ggcatcgctg
     1441 tcggcctggc aacaaaaatt cttcctcata attttaatga attattggat gcttgcgtgg
     1501 cctatttgag aaatgaacct ttcgtattat acccggactt cttaaccggt ggctttgccg
     1561 atgttagtaa ttataattca gggaatcggg gaggccgtgt cttgtcaagg gcgaaaatag
     1621 agatacccaa caggaatatg ctcgaaatca ctgaactacc attcggggtg actaccgacc
     1681 gattgataga gagtatattg aaagcaaatg acaaaggaca aatcaaaatc aaaaaaatca
     1741 cggataatac tgccagggat gtcaatattt ctattgagct gatgccaggg atttctcccg
     1801 atcaaacgat ggatgccttg tatcgatttt ctgactgtga ggtaagcatt tccaccatgt
     1861 gctgtatcat ccatgatgac aagccggtct ttataggagt tgaagaggtt cttaaagcta
     1921 atgttgatca aacgaaacaa cttctaaaac aagaattgca gatcaaactg gatgagctta
     1981 ctgaaaagtg gttctttacc agccttgaaa aaatatttat agagcaaagg atctaccggg
     2041 atattgagga agcggaaagc ttcgaacaag tgatttctat cattcgtgca ggattggaaa
     2101 aatatgtatc tacgccgact catcccgaac cgactatcaa attgctggcc aaattactac
     2161 gggatatcaa tgaagatgat atcatccgtc ttacagaaat taagataaag aaaatctcca
     2221 aatacagcag ttttgaaact gatcaggcgt tgaaaactct cctggaagaa atctccaagg
     2281 tgaaacacga tctcaaacat ctggttgaat tcaccattca atggtttacc tatctgaagg
     2341 acaaatacgg taagggtaaa gaaagaaaga ccatcctgac ccagtttgaa gccattgaag
     2401 cgacagcggt cgtccagaat aattccaagt tgtatgtcaa cagggaagag ggctttgtag
     2461 gtttcggcct caagaaggat gagtttgtat ctgattgctc ggatatcgat gacatcatcg
     2521 tctttactgc gcagggcaag atgatggtta caaggctgtc cgagaaaacc ttcgtaggta
     2581 ccaatataat acatgtggca gtttggaaga aaaatgatga gcggactacc tataatatga
     2641 tctattcgga tggtccggga ggaaaggcct atgccaagag attcaatgtc acggctatta
     2701 cacgggataa agagtatgat cttaccaagg gtgctgcaaa gcccagactg cattattttt
     2761 cagcaaatga gaatggtgaa gcagaaattg ttaaggtaac tctttctcaa acatctaaag
     2821 cgaagataaa ggtgtttgat ttcgacttta gtacgcaact gatcaagggg cgtgattctc
     2881 aaggtaatat cgtcacaaaa tatccggtca gaaaaatcac actggaggaa aaaggtggtt
     2941 cgacgctcgg cgcacaggta ctttattttg atgaagaaac cggaaaactc aatgtggatg
     3001 ataaaggtaa aaagattgga agtattgaca acggagacaa actggtaata ttttattcca
     3061 atggtagcct ggagcttgtt cctgttgatt tgtccttaaa gctgaatgcc agggaaatac
     3121 tgaatattgc cattttcaat ccggaaatga ttgttaatgt agtccatttc gatggccaca
     3181 agg
//
LOCUS       DAOQQN010000358         3179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_15483_length_3179_cov_5.866517, whole genome shotgun sequence.
ACCESSION   DAOQQN010000358 DAOQQN010000000
VERSION     DAOQQN010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15483_length_3179_cov_5.866517"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1493
                     /locus_tag="PLG24_11520"
     CDS             <1..1493
                     /locus_tag="PLG24_11520"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HMZ25567.1"
                     /translation="RQNSLIFVLGLLAVFSISCEKEFLDRKPIIGVTSNNFYKTEADA
                     LAAVNAAYATLQFELSPAGHFRWFWGDIMSDDSQKGGSGDNDVVDLMKLETFQGPTNT
                     DLLESEWNADYEGIYRANVVLEKVPGIEMDEVLKKRILAEAKFIRAWFYYNLNTIFGG
                     VPLADKVLAPSEYNRPKEDAAVIWAFIEKDLKEAIEDLPQRSQYQAKDLGRITKGAAR
                     ALLAKAYLYQGKYAEAGFQTGEIIQSGEYSLVADYGTIFTKAGENNAESVFEIQYMNT
                     SGGNWGKNAANEGTFTNVFQRARGQFAGYGFNIPTQDFVDEFFKEGFEDPRLKYTVFR
                     IGDQMGDRGEFTVANAGDVGYIYHPRKYFTNQSEDAPFGDPNPNGESNDRVIRYADVL
                     LMDAEAAAKTGDETKARESLNRVRMRVGVPAITASSSALLDAIFRERRIELGLEGHRF
                     FDLVRTGRAAEKLGPLGFKDGVHNIFPIPNSEIVLSNGILKQNPGY"
     gene            1514..2344
                     /locus_tag="PLG24_11525"
     CDS             1514..2344
                     /locus_tag="PLG24_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25568.1"
                     /translation="MKNIFIVIIGLFIFQGCSPDNNDINLGPLPPKPDFSIEYVPGDS
                     NKVVVKDLSSDSFVRLWEIGEGGIPATSSLESDTIFFSKKGEYDISLYISQKGGNGTA
                     YNTKKISIPKDVVLPCSGPLGLLTGNCSTTGKCWKFSDVAGAITVGETYGAANWYKSP
                     ANGLVASQYDDRYCFVFEGFKFDYRNNGSTVNPFKGYVDEPFIPVSGPFAFSPGTGPN
                     GEDQIILAKGQFMGTMDSYSVLNIIKLTETELIVRAQFADAAGNPVTPGWFEFKYVAD
                     "
     gene            2344..3135
                     /locus_tag="PLG24_11530"
     CDS             2344..3135
                     /locus_tag="PLG24_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HMZ25569.1"
                     /translation="MQKLFFIFLFLSAWLVSFGQYGRLVWQDEFESQSPVPDSTKWTF
                     DLGDGCPSNCGWGNNQKEYYTNDLRNARIEEGRLILEAHRNNRSGHPYTSARITTRKS
                     LNLRYGRIDVRARLPRGHGTWPAIWMLPSKWEYGGWPRSGEIDIMEHVGWAQDSIFGT
                     VHTQNYNHIIGTQQGKTVQLPDASLYFNDYSIIWTEEKIDFLINGKVFYTYKNDSTGP
                     DSWPFDKEFYLIMNLAIGGNFGGVKGVDDDIWPQRMEVEYVRVYN"
BASE COUNT          920 a          616 c          815 g          828 t
ORIGIN      
        1 aaagacaaaa tagcttgatt tttgtattag ggctgttggc cgtgttcagc atttcttgtg
       61 aaaaggagtt ccttgatcga aagcccatca tcggtgtcac atccaacaac ttttacaaaa
      121 cagaggcgga cgctttggca gcagtaaatg ccgcctatgc cacactacag tttgaattaa
      181 gtccggcagg acatttccgc tggttttggg gcgatattat gtcggatgat tctcagaaag
      241 gaggctccgg ggataatgat gttgtggatt tgatgaaact agaaacattt cagggaccga
      301 ccaatacaga tttacttgag tcggaatgga atgctgacta cgaaggaatc taccgtgcca
      361 atgttgtatt ggaaaaggtg ccgggaatag aaatggacga ggtgttgaaa aagagaatcc
      421 tggcagaagc caaatttatt cgagcctggt tttattacaa tctaaatacc atttttggtg
      481 gggttccact ggcggacaag gtcctggcgc catcggaata caacagaccg aaggaagatg
      541 ccgctgtcat ttgggcattc atagagaaag atctgaaaga ggccattgaa gacctgcctc
      601 aaaggtcaca atatcaggcg aaagacttag ggcggattac gaagggagca gcaagggcct
      661 tgcttgccaa agcgtatcta tatcaaggta agtatgcaga ggcagggttt cagacaggag
      721 agattatcca atccggagaa tactcgttgg tagcagatta tggaacgata tttaccaagg
      781 ctggcgagaa caatgcagaa tcggtctttg agattcaata tatgaacaca tcggggggca
      841 attggggtaa aaatgcggcc aatgaaggta cctttaccaa tgtattccag cgtgccagag
      901 gacaatttgc cggctatggc ttcaatattc cgacacagga ttttgttgat gaattcttca
      961 aagagggttt tgaggatccc cgccttaaat atacggtgtt caggattggt gatcagatgg
     1021 gagatcgggg tgaatttacg gttgccaatg ccggagatgt aggttatatc tatcatccaa
     1081 gaaaatactt caccaatcaa agtgaagatg ctccttttgg cgatcccaat cccaatggcg
     1141 agagtaatga ccgcgtcatc agatatgccg atgtcctgtt gatggatgca gaagcagctg
     1201 ctaaaaccgg agatgaaacc aaggcgcgag agtcactgaa ccgggtaaga atgcgggtag
     1261 gtgtgccggc tattacagct tcaagcagtg cattgctgga tgctattttt cgggaaagaa
     1321 gaattgagct gggcctggaa gggcatcgtt tctttgacct ggtacgcacc ggaagagcag
     1381 ctgaaaaatt aggcccactg ggctttaagg acggggtaca caatatattc ccgataccga
     1441 atagcgaaat cgttttatcc aacggtatat tgaaacaaaa tccgggttat tgattcaatc
     1501 aaattcattc atgatgaaaa atatatttat agttataata ggactcttca tttttcaggg
     1561 gtgcagcccg gataacaacg acatcaatct ggggccgttg ccgccaaagc cggatttctc
     1621 aattgaatat gtacccggtg atagcaataa ggttgtggtt aaagacttat cttctgatag
     1681 cttcgtacgc ttatgggaaa ttggcgaggg aggtataccg gcaacatcat ctctggagag
     1741 tgataccatt ttcttttcca aaaagggaga atatgacatt tccctttata tttcccaaaa
     1801 aggcggtaat ggtactgcat acaataccaa gaagatatct atacccaaag atgttgtgct
     1861 tccctgcagc ggaccacttg gcctgttgac cggcaattgc agcacgaccg gcaaatgctg
     1921 gaaattttcc gatgtagcag gagccattac agtaggagaa acatatggcg ctgccaattg
     1981 gtataaatct ccggctaatg gtttggtcgc ttcccagtat gatgaccgat attgttttgt
     2041 ctttgagggt ttcaaattcg actatcgcaa taatggcagc acggtcaatc cgttcaaggg
     2101 atatgttgat gagcccttta ttcctgtttc gggtccgttt gctttcagtc ctggcacagg
     2161 accaaatggt gaagatcaga taatcctggc caaagggcag tttatgggca cgatggatag
     2221 ttatagtgta ctaaatatta taaaactgac ggaaacagaa ttgattgtca gggctcaatt
     2281 tgctgatgcg gcaggcaatc ccgttacacc gggctggttt gaattcaaat atgtggcaga
     2341 ttgatgcaga agctcttctt tatattcctc tttttatcgg cctggcttgt gtcatttggc
     2401 caatatggaa ggcttgtatg gcaggatgaa tttgaaagtc aatctcccgt gccggattca
     2461 accaaatgga cttttgacct gggcgatggc tgcccatcaa attgcggatg gggcaacaat
     2521 cagaaagaat actatacgaa tgacctgagg aatgcgcgca ttgaggaggg acgattgatc
     2581 ctggaggcgc accggaataa ccgaagcggc cacccttata cttcggcgag gataacaacc
     2641 cgcaagtcac tcaatttaag atatggccgc atcgatgtca gagcacggct tccacgtgga
     2701 catggcacct ggcctgctat ctggatgcta ccgtcaaaat gggaatatgg agggtggccc
     2761 cgaagcggtg aaattgatat catggaacat gtgggatggg cacaggattc tattttcgga
     2821 acagtacata ctcagaatta caaccatatt atcggtacac aacaggggaa gacggtgcaa
     2881 ctgccggatg catcacttta tttcaacgat tattccatca tatggacaga agaaaaaatc
     2941 gatttcctca tcaatgggaa ggttttctac acctataaaa acgacagtac aggtccggat
     3001 agctggcctt ttgataagga attctattta ataatgaacc ttgccatagg aggtaatttc
     3061 ggaggagtca agggcgtaga tgatgatatt tggccgcagc gaatggaagt ggaatatgtt
     3121 agagtgtata attgatggca tatgaataga tttagaaaaa taagttatgc ttggctttg
//
LOCUS       DAOQQN010000359         3178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_15498_length_3178_cov_5.328850, whole genome shotgun sequence.
ACCESSION   DAOQQN010000359 DAOQQN010000000
VERSION     DAOQQN010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15498_length_3178_cov_5.328850"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1621)
                     /locus_tag="PLG24_11535"
     CDS             complement(<1..1621)
                     /locus_tag="PLG24_11535"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HMZ25570.1"
                     /translation="MNSFNRALNIAGWLTFAITFMVFVFAVERTGSLWDCGEFILGAY
                     KLQVVHPPGAPLFLMIGRMFAYIGSMFTSTPANIAFAVNLMSGLLTAFAGMFICWTTM
                     YLGKLSLVGRGNESDSGQNIGLLLAGLVAGLTSTFCISVWFSAMEGEVYAMSTFFTAL
                     TLWSLLKWYYLPDTQHSDRWIVFSLFMAGLSIGVHLLSILTFPALAIFYYLKKFNKFT
                     WTGLLISIVAGLVVLFIVQRVIIVGLPDLLVSLDIFAVNSLGLPFYSGLVPFFAIIGG
                     LLYWGIKKANSRGMHNLQLGLVSLMLIIVAYSTTTVAVIRANANPPINMNNPSDPARL
                     VPYLNREQYGDRPLLFGPSFDAKPIGLKSEPRYGRVGDKYEVIDMKYEYEYKPEDNMF
                     FPRMGHGDDPTKVRLYKQWMGHDGKPTMGDNLSFFWRYQLGWMYWRYFMWNFVGKQNG
                     DQGYVPWDKSKGNWLSGISFIDNTRLGNQDQLPSFEKNNQARNKYYFLPFILGIIGTI
                     FHLKRRRNDWIGLFMLFIITGIGIIVYSNQPP"
     gene            complement(1832..3169)
                     /locus_tag="PLG24_11540"
     CDS             complement(1832..3169)
                     /locus_tag="PLG24_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ25571.1"
                     /translation="MIILIIISIIQKRNRPKGVSVTTELVAKHDIREIVTASGKIFPE
                     NEVKISSDVSGEIIALYVREGDTVKAGQLLAKINADTYLPSVQRGEASVKSSKAQENI
                     SDTQIKAAKAQQEEVNARLANAQKVHERNKKLFEDGVISQADLEASQSSLDGLIASAK
                     SAAASLRQAQENASASKYGTMSARANLDELKTSLSKTFIYAPVDGVISSLNVEKGERV
                     VGTIQMSGTEMMTIANLNSIEVQVEVSENDIVRVEKGNPATIEVDAFYGRKFNGVVTE
                     IANSSTASSSTNTTASLNTDQVTNFIVKIRMDLNSYKDMIIKAKPFPFRPGMSASAEI
                     NTRTEHDVLSVPIQAVTTREDPSGKRNEKSGKKADLIEVVFLQQGDTVNMVPVKTGIQ
                     DDEYIHILSGLKEKDRVVTGPYSTVSRTLKQGSVVHEDTKKKGLDKEDKEEEQ"
BASE COUNT          855 a          771 c          655 g          897 t
ORIGIN      
        1 taggtggttg attggaatac acaataattc caataccggt tatgataaat agcatgaata
       61 aacctatcca atcattcctc ctcctcttta aatggaagat ggttccaatg atgcccagaa
      121 taaatggcag gaagtaatat ttgtttcttg cctgattgtt cttttcaaag gaagggagct
      181 gatcctgatt gcccaaccgc gtattgtcga tgaatgaaat accgctcaac cagttgccct
      241 tggacttatc ccatggcaca taaccctgat ctccattctg cttgccgaca aagttccaca
      301 tgaaatatcg ccagtacatc catcccagct gatagcgcca gaagaaggaa agattgtcgc
      361 ccatagtagg cttgccatca tgccccatcc attgcttgta gagtctaacc ttggtcggat
      421 catcaccatg tcccatacgt gggaagaaca tattatcctc aggcttgtac tcatattcat
      481 atttcatatc gatcacttca tatttgtcac ctacacgccc ataacgcggc tcactcttga
      541 gtccgatggg cttggcatca aaggaaggtc caaaaagcaa cggcctgtcg ccatactgct
      601 ctctgttaag gtatggaacg agccgggcag ggtctgaagg attgttcata ttgatgggtg
      661 ggttggcatt ggcccttatc acagccactg ttgtagttga ataggcaact ataatgagca
      721 tcaagctgac aagacccagc tgaagattgt gcataccacg tgaattggcc ttttttatcc
      781 cccaataaag gagaccgcca atgatggcaa agaacggtac aagtcctgaa tagaaaggca
      841 aacccaatga gttgactgca aatatgtcca gtgaaaccaa caagtcggga agacctacaa
      901 tgataacgcg ttgtactatg aagagcacga caagaccggc aacaattgaa atcaaaagcc
      961 cggtccatgt aaatttattg aactttttga ggtaataaaa gatggccaaa gccgggaatg
     1021 tcaatatact caacaagtga acccctatgg aaagaccagc cataaaaagg gaaaatacta
     1081 tccatcggtc actgtgttgt gtgtctggaa gataatacca tttcaataag gaccacaatg
     1141 tcaatgctgt aaaaaaagta gacatggcat atacctcgcc ttccatcgcc gagaaccaga
     1201 ctgaaataca gaaagtggaa gtaagtccgg caactaatcc agccagcaat aatccaatgt
     1261 tctgcccgga gtcggactca ttgccccttc ctactaagga caatttaccc aggtacatgg
     1321 tcgtccagca aataaacatt ccggcaaatg ctgtaagaag gcctgacata aggtttacag
     1381 caaacgcaat attggccggt gtggaggtaa acatactgcc tatatacgca aacattcttc
     1441 ctatcatcag gaataaaggc gctcccggtg ggtgcaccac ctgaagttta tacgctccta
     1501 gaataaattc accgcaatcc catagacttc cggtcctctc gacggcaaaa acgaaaacca
     1561 tgaaggtgat tgcaaatgtt agccagccag caatatttaa ggctctgttg aaactattca
     1621 tccgttaact ttcagtttat aaaaaaaggc aactactcaa tgccacagga ttccaccttt
     1681 agcgaacagc actgtatcct tccggggtat acccgactaa agggcaaaaa aacctggtgg
     1741 caaagttaag ccctttcttt tattggacaa attatcctct ctttttataa cgtagctatc
     1801 ggtgattata tattaatatt aagttaacac gttattgttc ctcctcttta tcctccttgt
     1861 ctaacccttt ctttttcgta tcttcatgta ccacactacc ctgtttcaag gtccttgaca
     1921 cggtactata cggtccggtc accaccctgt ctttttcctt caatccactc agaatgtgga
     1981 tatactcatc gtcctgtatt ccggtcttca ccggtaccat attgacagta tccccttgtt
     2041 gaaggaagac aacctctata aggtcggcct tctttccgct tttttcattt cttttgcctg
     2101 aagggtcttc ccttgttgtc acggcctgaa tgggcacgga cagaacatcg tgttcagttc
     2161 ttgtatttat ctctgccgat gctgacatgc ctggtcggaa ggggaagggt ttggccttta
     2221 taatcatatc cttgtaggag ttgaggtcca ttctgatctt tacaataaaa ttggtcacct
     2281 ggtccgtatt cagggaagca gtagtgtttg tactgctgga agcggtggag ctgttggcaa
     2341 tctctgttac aacaccatta aattttctgc catagaaggc atccacttcg attgttgcag
     2401 ggttgccctt ttctacccgg acgatatcat tttctgaaac ctccacctgc acttctatgg
     2461 agttcagatt ggcaatggtc atcatctctg tgcctgacat ctggattgtt cccacaaccc
     2521 gctcgccttt ttcgacattt agagaactga tgacaccatc gacaggagcg tagataaaag
     2581 tcttgctgag gctggttttt agctcatcca ggttagccct ggcactcatg gttccgtatt
     2641 tcgaagccga ggcgttttcc tgtgcctgtc taagggaagc agcagctgac ttggcagatg
     2701 ctatgaggcc atccaaactg gactgggacg cctccaaatc tgcctgactg attaccccgt
     2761 cttcaaacaa cttcttattc ctttcatgca ctttttgtgc attggcaaga cgtgcattga
     2821 cctcttcctg ttgtgctttg gcagccttaa tctgagtgtc ggaaatgttt tcctgtgcct
     2881 ttgaactctt aacagatgcc tcaccacgct ggacggatgg aaggtaggta tcggcattga
     2941 tcttggccag caattgacct gcttttaccg tatctccttc acgcacatac aaggcaataa
     3001 tctctccgga cacatccgaa cttatcttca cttcattctc cgggaaaatt ttgccggaag
     3061 ctgttacaat ttcccggatg tcgtgcttag cgacgagttc agtcgtcaca ctgactcctt
     3121 tcggcctatt tcttttttgg attatggaga taattatcaa tattatcacg atgcctcc
//
LOCUS       DAOQQN010000360         3170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_15559_length_3170_cov_5.202247, whole genome shotgun sequence.
ACCESSION   DAOQQN010000360 DAOQQN010000000
VERSION     DAOQQN010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15559_length_3170_cov_5.202247"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2221
                     /locus_tag="PLG24_11545"
     CDS             <1..2221
                     /locus_tag="PLG24_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMZ25572.1"
                     /translation="FWSCHRDDIDLEIQKIDKPSALIYEQVNATVYGYVYDEAGQPVA
                     NALVQMYSTNATTNAKGLFVFKAEKMDQLGTYIRVIKEGYLTASDYLFPKPGSKEFSY
                     IRMIRDKQDKYFESSVGGTISLEGGGTITFPPNAIKKEDGGLYSGKVTLTARFIDPTS
                     PHFGDEMPGGLIGIDINGNRVVMGTGGMFAVELRSPSGEKLNLREGSKAEYSIPARSK
                     AKPASMPTWWFDVKDGLWKEEGRAVLVNGHYTGQVSHFSFWNVDAKFDLVRVCGTVVD
                     SEGKPMDQAIVVVEAKLDDGKLVTGYGYTDVDGNYCGWMPKGVELHITVRPVICPDKA
                     KEVVVGPFYNNASINNIVVDAQKKPVSVKVTCNGVPAGDAIVVLEQNGKTILVDVPNG
                     ASTFNLYNYTCDDNSPISIFGFEQKTGKTSSDQAFNFANGVYEFDVCQSGCSLSGVFI
                     TDCDPIIIKVSGGSGQYKYQWNTGDTTDRFSIKSADAKLYSVTVTDIQNPTCSQVFTK
                     NLPGLISCTIELTDCHWPISLRVKGSNYNKIKWSNGHDGVSQDVQPDHTTTYSVTVTN
                     EIGCSSTASIVVDPDLFPYVSSARTCSKNLITLPGRFKSGTIQGFNGFSKNLNSPSDL
                     VDLNIFETGYVLWGYVNNGKGCEQPFEFNLPQYDGLKVLNFSTGNVIDGNIIKYTASN
                     GSCYNCDAGDVAIFKASDLNTDLVQLNATGLPGGEYYVVLKDANSGCFVAFVKVKII"
     gene            2223..2795
                     /locus_tag="PLG24_11550"
     CDS             2223..2795
                     /locus_tag="PLG24_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ25573.1"
                     /translation="MDIDNILQGCLQQQQSSQELLVHAFAPKLLSICERYCKDKEMAR
                     DAVQETFINIFKYIHTYNGETAFITWARKIAVNNCLKINKKYFKLQFVDEAEARFNHG
                     SYIPEAYSNLSVESIMELVRKLPDSQYVVFNMYVVEGFSHKEIGEALNITESSSRSTL
                     TRARSKLIEMINDIEDKNNNSIFNSRIALL"
     gene            2792..>3170
                     /locus_tag="PLG24_11555"
     CDS             2792..>3170
                     /locus_tag="PLG24_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25574.1"
                     /translation="MNYKEFEKNIKNKLSEHEAQIDTNALVSAIFLKKKKGKRFPAWW
                     GYVGALIVVTLVSTLWIFDSRNNDLSYKDRAATNPTAESEQPGLTYDDVYDRTPTDGL
                     NTKDNSVKNEMVASLASGNNLDVN"
BASE COUNT          925 a          574 c          758 g          913 t
ORIGIN      
        1 tttttggtcg tgccatagag atgacattga tttagaaatt cagaaaatcg ataagccttc
       61 tgcactgatc tacgaacaag tgaatgctac cgtatacgga tatgtctatg atgaggccgg
      121 ccaacctgtt gctaatgcac tggtacagat gtatagcaca aacgcgacta ccaatgcaaa
      181 aggcttgttt gtcttcaagg ctgaaaaaat ggatcaattg ggtacataca tacgggtaat
      241 taaggaagga tatctcactg cgagtgacta tttatttccc aagcccggaa gcaaggaatt
      301 ctcttacatt cgtatgatac gcgacaagca ggataaatac tttgaatctt ctgttggagg
      361 taccatctca ttggaaggtg gtgggacgat tacattcccc cccaacgcca tcaaaaagga
      421 agatggcggg ttgtattcag gtaaggtgac tttgacggcg cgttttatcg atccaacttc
      481 tcctcatttt ggtgatgaaa tgccgggtgg cctgattgga attgatatca atggcaatcg
      541 ggttgtgatg gggacaggtg gtatgtttgc cgttgagttg aggtctccct ccggtgagaa
      601 attgaacctt agagaaggct caaaagctga atattctatt cctgccaggt ctaaggccaa
      661 acctgcatct atgcctacct ggtggtttga tgtcaaagac ggactttgga aggaagaagg
      721 acgggcagtc cttgtcaatg gtcattatac aggacaggtt tcccatttta gtttttggaa
      781 tgtggatgcc aagttcgatc ttgtaagagt gtgtggtacg gtggttgatt ctgagggtaa
      841 gcctatggat caagccattg tcgttgttga agcaaaattg gatgatggca aattagtaac
      901 aggttatggt tatactgacg ttgacggtaa ttattgtgga tggatgccta agggagttga
      961 gttgcacatc accgtccgac cggttatttg tcctgacaag gcgaaggaag tcgttgttgg
     1021 cccgttttac aacaatgcga gtattaacaa cattgtggtt gatgcccaga aaaagccagt
     1081 ctctgttaag gttacctgca acggagttcc tgccggagac gcgatagtag tacttgagca
     1141 gaatggtaaa acgatcctgg ttgatgtccc caatggagcc tctacattca acttgtataa
     1201 ttatacgtgt gatgacaact cgcccataag tatattcggc tttgagcaaa agaccggtaa
     1261 aacttcgtct gatcaggctt ttaactttgc aaatggcgta tatgaatttg atgtttgtca
     1321 atcaggatgt tcattgtcag gtgtgtttat taccgattgt gatccgatta ttattaaggt
     1381 ttcaggaggt agtggacaat acaaatatca atggaatacc ggtgatacta cggatagatt
     1441 cagtattaaa agtgccgatg ctaaattgta ttccgtaact gtaaccgata ttcagaatcc
     1501 cacttgtagt caggtgttta ctaagaatct acccggtctc atctcatgca caattgaatt
     1561 gaccgattgc cactggccga tatcactcag agtaaagggt tccaattata ataaaatcaa
     1621 atggtctaat ggacatgatg gggtaagcca ggatgtccaa cctgatcata caacaaccta
     1681 ttctgtcacc gtaacgaatg aaattggttg ctcatccact gcttctattg tagtagatcc
     1741 tgatttgttc ccctatgtga gttcggcaag gacgtgcagc aagaatctga tcaccttacc
     1801 aggtaggttc aagtcgggaa ctattcaggg atttaatggc ttttcaaaaa acctgaatag
     1861 tccgtctgat ctggtcgacc tgaatatttt tgaaaccggt tatgtacttt ggggctatgt
     1921 gaataacggt aagggatgtg agcagccttt cgagttcaac ctgccccagt atgatggttt
     1981 gaaggtgttg aatttcagta caggaaatgt tatagatggt aatattataa agtacacagc
     2041 cagtaacggg agttgctata attgtgacgc aggtgatgta gctatcttca aggcgagcga
     2101 tctcaatact gatctggtac agttgaatgc caccggcttg cctggcggag aatattatgt
     2161 tgtattaaag gatgcgaatt cgggatgttt tgtagcattt gttaaagtga aaataatata
     2221 gggtggatat tgacaatata ctccaaggct gtcttcaaca acagcaaagt agtcaagagc
     2281 ttttggtgca tgcatttgca ccaaagcttt tgtctatctg tgagagatat tgtaaggata
     2341 aggaaatggc ccgcgatgct gtgcaagaaa ccttcattaa tatttttaaa tacatccaca
     2401 cctacaatgg agaaacagca tttataacat gggcaagaaa gatagccgtc aataattgtc
     2461 tgaaaataaa taaaaaatat ttcaagttgc aatttgtcga tgaagctgaa gcccgattca
     2521 atcatggctc atatatcccg gaggcttaca gtaacctatc ggtggagtcc attatggaat
     2581 tagtacgaaa actgccggat agccaatacg ttgtatttaa tatgtatgtg gtggaagggt
     2641 ttagccataa agagatcggg gaagcattga atattacgga aagtagttcg agatcgactc
     2701 ttacccgtgc acgcagcaag cttatcgaga tgattaatga tattgaagat aaaaacaata
     2761 attcgatttt taattccaga atcgctctgt tatgaattac aaggaattcg aaaagaatat
     2821 taaaaataag ttatctgaac atgaggctca gatagatacc aatgcgctgg tttcagcgat
     2881 ttttttaaaa aagaaaaagg gcaaacgctt cccggcctgg tggggctatg ttggggcttt
     2941 gattgttgtt acccttgttt cgacgctctg gatctttgat tcgaggaata atgatctttc
     3001 gtacaaggat agggccgcta cgaatcctac cgccgaatct gagcaaccgg gccttactta
     3061 cgatgatgtc tatgaccgga cacccacaga tgggctaaat actaaggaca actctgtcaa
     3121 aaatgagatg gtggcatcgc tagcctccgg caataatttg gatgttaatt
//
LOCUS       DAOQQN010000361         3143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_15772_length_3143_cov_3.830635, whole genome shotgun sequence.
ACCESSION   DAOQQN010000361 DAOQQN010000000
VERSION     DAOQQN010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15772_length_3143_cov_3.830635"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..567)
                     /gene="rfbC"
                     /locus_tag="PLG24_11560"
     CDS             complement(19..567)
                     /gene="rfbC"
                     /locus_tag="PLG24_11560"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HMZ25575.1"
                     /translation="MPISTVDIEGILIFTPRIFTDDRGYFYESFRADQYADFGIIKPF
                     VQENQAYSTRGALRGLHYQTGEMAQAKLVRVIVGEVLDVAVDLRPTSKTYGKVYSIIL
                     SGDNHKQMYVPRGFAHGYLVLSDEAIFTYKCDNYYSKQHEGGIRYDDPLLNIDWQMED
                     AELILSEKDLELPYFGNHMPAI"
     gene            complement(573..1874)
                     /gene="der"
                     /locus_tag="PLG24_11565"
     CDS             complement(573..1874)
                     /gene="der"
                     /locus_tag="PLG24_11565"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HMZ25576.1"
                     /translation="MSNIVAIVGRPNVGKSTLFNRLIGKKQAIIDDVSGVTRDRQYGE
                     AVWNGKSFIVVDTGGFVQGSDDVFESAIRDQVRIAVAEASAIIFIVDVETGETDLDEK
                     VANMLRQSGKNVILVVNKVDNSNRYIEAQEFWGMGFKHVHFISSMTGSGTGELLDELI
                     PMIGEAEQLDTDLPKIAIIGQPNVGKSSYTNVLLGEERNIVTEIAGTTRDSIHTRYNL
                     FNKDFYIIDTAGIRKKQRVNEDLEFYSVMRSIRAIEEADVCALLVDAQTGIEAQDMSI
                     FRLVQKRNKGIAIFVNKWDLVEKETNTARDFEKAIKAKLAPFNDVPVVFISALEKTRI
                     FKSIEVLLRVFDNKIKKIATSKLNEIMLPIIEQTPPPAYRGKMIKIKYITQLSASHPV
                     FGFFCNHPDHIKESYKLFLENQIRKHFDFTGVKISIVFKEK"
     gene            2010..2666
                     /locus_tag="PLG24_11570"
     CDS             2010..2666
                     /locus_tag="PLG24_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ25577.1"
                     /translation="MSKPKIQVLVVDDHKMFVEGVQAIFANSPEIHIAGTIFDGKEVM
                     AAIENTSGLDIILLDINLPNINGLELTRMISKKYPNIKILILSMYNNAEYIREVLKEG
                     ASGYVLKNTDHEELLNAIQSVYAGNSFYSQSVTQTMMNSFAKKQQGTNMDIMQVKISK
                     REKEILGLIIKEYTAQEIANMLFISLHTVETHRSNLMSKLGVRNSAGLVRVALEHGLV
                     "
     gene            2683..>3143
                     /locus_tag="PLG24_11575"
     CDS             2683..>3143
                     /locus_tag="PLG24_11575"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ25578.1"
                     /translation="MAELGCNIILIKNKLLSLLPVLMIVSVTYYPSWGNHENLLLTDS
                     LKNSLITGLNSIKKSGSDEQKASFYQKLAEYYEGQSRQDSAISYYQKSSIHYNQAGND
                     IMYYEILAKLGSLNYNVFNYKFAIEYFREVTDYYKRKKMYLLSTVVIAIGF"
BASE COUNT          932 a          681 c          605 g          925 t
ORIGIN      
        1 tgattgcttt tatgttattc atattgcggg catatggttt ccaaaatagg gtaattcaag
       61 atccttttcg cttagtatca attcagcatc ttccatttgc caatctatat tgaggagtgg
      121 atcatcataa cggatgccgc cctcatgttg tttgctgtaa taattgtcac acttataggt
      181 aaatatcgct tcatccgata aaacaagata tccatgggca aatccgcgtg gtacatacat
      241 ttgtttgtgg ttatcaccac tcaaaataat ggaataaacc ttcccgtaag tcttggaagt
      301 cggcctaaga tccaccgcca catcaagtac ttcgcccaca atgaccctga ctagtttggc
      361 ttgtgccatt tcgcctgtct ggtaatgcaa tcctctgaga gcgcctctgg tcgaataagc
      421 ctgattctct tgtacaaatg gcttgatgat gccaaaatct gcatactggt ctgcccgaaa
      481 actctcataa aaataccccc ggtcatccgt aaaaattctg ggagtaaaaa tgagaattcc
      541 ttcaatatct acagtactaa tgggcataca gcttattttt ccttaaagac aatgcttatt
      601 tttacacctg taaaatcaaa atgcttccta atctgattct ccagaaatag cttatagctt
      661 tccttgatat ggtcgggatg attgcagaaa aatccgaata ccggatgact tgcagacagt
      721 tgggtgatgt actttatttt gatcatcttg ccacgatatg cagggggtgg cgtctgctct
      781 atgatgggta gcatgatttc attaagcttt gaagtggcaa tcttcttaat tttattatca
      841 aatacgcgaa gcaaaacttc aattgatttg aaaatccttg ttttttccaa agctgaaatg
      901 aatactacgg gcacatcgtt gaatggagcc aatttagcct tgatggcctt ctcaaaatcg
      961 cgtgcagtgt tggtttcctt ttctacaaga tcccatttgt tgacaaagat ggcgatgcct
     1021 ttgttccttt tttgaacaag acgaaaaata ctcatatctt gagcctcgat gccagtctga
     1081 gcatcgacga gtaaggcgca gacatcagct tcttcaatag cacggataga gcgcatcacg
     1141 gaataaaatt caagatcttc attgacacgt tgcttttttc gaattccggc cgtatctatg
     1201 atgtaaaaat ccttattaaa tagattgtat cttgtgtgaa tagagtccct cgtcgtacct
     1261 gcaatctccg ttacgatgtt gcgctcttcg cctagcaaga cattggtgta gcttgacttt
     1321 ccgacattgg gctgaccgat gatggctatt ttaggcagat ccgtatccaa ttgctccgcc
     1381 tccccaatca tgggaatcag ttcgtccagt aattcaccgg ttccactccc ggtcattgaa
     1441 ctaataaagt ggacatgctt aaatcccatc ccccagaatt cctgcgcttc gatgtaacga
     1501 ttggaattat ccaccttatt gaccaccagg ataacatttt ttccggattg cctcaacatg
     1561 ttggcgactt tttcatccag gtcagtttca ccggtctcca catccacgat gaatatgatt
     1621 gccgaagcct cagcaaccgc aattcttacc tgatcacgga tcgcactttc aaacacatca
     1681 tccgaaccct gaacaaagcc tccggtatct actactataa atgacttacc attccagact
     1741 gcctccccat attgcctgtc gcgtgtaaca ccactcacgt catcaatgat ggcttgtttc
     1801 ttaccgatca gacgattgaa cagcgttgat ttacctacat tcggacggcc tactatggct
     1861 actatattac tcattaaaaa tatttatcaa agtgcgaaag tataataaat taagcgaatt
     1921 aatatatgag aatcaaaaat ttgacgtaat ttggcactta tttaggcctt tccctgtcca
     1981 atattatact caaatagttg atttgatcta tgtcaaaacc aaaaatccag gttctcgtgg
     2041 ttgatgacca taaaatgttt gtagagggag tacaggcaat ttttgccaat tctcctgaaa
     2101 ttcatattgc agggactata tttgatggaa aggaagtcat ggcagccatt gagaatacat
     2161 caggactgga catcatcctc ctggacatca atcttcccaa cattaacggg ctggaactta
     2221 cccgaatgat cagtaaaaaa tacccgaaca tcaagatact gatcctgagc atgtacaaca
     2281 acgccgaata catcagggaa gtactcaagg aaggagcatc gggttatgtc ctcaaaaaca
     2341 ccgaccacga agaattactg aatgccattc aatccgtata tgccggcaat tccttttaca
     2401 gtcaatccgt tactcagacc atgatgaata gttttgccaa aaaacaacaa ggtacaaata
     2461 tggatatcat gcaggttaag atttcaaaac gggaaaaaga gatcctcggc ctgatcatca
     2521 aagaatatac cgctcaagag atcgctaata tgttgttcat ctcactgcat accgtggaga
     2581 cgcatcgcag caatctaatg tccaaactgg gagtccgcaa tagtgcagga ctggtcagag
     2641 tggctcttga acatggattg gtttaatcag tgatgggacg atatggcgga attgggctgt
     2701 aatattattc ttataaaaaa caagcttctc tcgttgttgc cggtattgat gattgtttcg
     2761 gtgacctact atccctcatg gggtaatcat gaaaatttac ttttgacgga ttcgttgaaa
     2821 aattcactca tcacaggatt aaattccatc aagaaatcgg gaagtgatga gcagaaagca
     2881 tcattctacc aaaagctcgc tgaatattat gaagggcagt cgcgccagga cagtgccata
     2941 agttattatc agaagagctc cattcattat aatcaggccg gaaatgatat aatgtactat
     3001 gaaattctgg caaaactagg atctctgaat tataatgtat tcaactacaa atttgcaatc
     3061 gaatatttca gggaggtaac ggattattac aagagaaaga agatgtatct tctttcaact
     3121 gtcgtaattg ccatcggatt cat
//
LOCUS       DAOQQN010000362         3134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_15844_length_3134_cov_5.064956, whole genome shotgun sequence.
ACCESSION   DAOQQN010000362 DAOQQN010000000
VERSION     DAOQQN010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15844_length_3134_cov_5.064956"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3083)
                     /gene="purL"
                     /locus_tag="PLG24_11580"
                     /gene_synonym="purI"
     CDS             complement(<1..3083)
                     /gene="purL"
                     /locus_tag="PLG24_11580"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF003672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HMZ25579.1"
                     /translation="MIFFFEGSGNTVYALQTENALSDSDISKLSWLFGDGRLIESKVV
                     EGTFVGPRATMVTPWSTNAVEITQNMAIEGITRIEEFVRSESDHPSYDPMLSAKYNSL
                     GQDIYSVRSKPEPILEITDIATYNETEGLALSEEEILYLNDLSDRLGRPLTDSEVFGF
                     SQVNSEHCRHKIFNGTFVIDGEEQPDSLFRMIKKTSQANPNSIVSAYKDNVAFLKGPI
                     IRQFAPKRPDVPEYFEIKEVASVLSLKAETHNFPTTVEPFNGAATGSGGEIRDRLAGG
                     QGALPLAGTAVYMTSYSRLSDNRDWEKANPERKWLYQTPLDILIKASNGASDFGNKFG
                     QPLICGSVLTFEHKEADRTLGYDKVIMQAGGIGFGKAGQAKKHIPAAGDKIIVMGGEN
                     YRIGMGGAAVSSADTGEFGSGIELNAVQRSNPEMQKRVANAIRGMVESDHNHILSIHD
                     HGAGGHLNCLSELVEQTGGFIDLDKLPVGDPTLSAREIIGNESQERMGLVIADSHIDT
                     LNKVAERERAPMYIVGKVSNDHRFTIASSSTGARPMDLKLGDMFGSSPKTIMEDKTID
                     RQYSPLIYDRKRIDEYLESVLRLEAVACKDWLTNKVDRCVGGLVAVQQCAGPLQLPLN
                     NCGVMALDYSGRDGIATSVGHSPLTALIDSAAGSRNAIAEALTNIVWAPLQDGIRSIS
                     LSANWMWPCKNEGEDARLYNAVRACSDFAIALGINIPTGKDSLSMKQKYPEGEVLAPG
                     TVIISAAGHCNDVRKVVGPVLKKEDAGIYYINFSGMSPRLGGSSFAQVLNRVGDTAPD
                     IADASEFVEKFAVIQSLVSQGLISAGHDIGSGGLITTLLEMCFADRGLGAEIDLSALP
                     GEDIIHKLFAENAGVVIQASSVAEEILIGAGVEFFPIGKIRREATLQVRDHEDSHTFD
                     IDRLRDIWFETSYLLDIRQSGPKKAKERYDNYKHHLLHYKFPASFDGKLPHIDSARPR
                     PKAAVLREKGSNSERELANALYSAGFDVKDVHMTDLISGRETLDDIQMIGAVGGFS"
BASE COUNT          765 a          803 c          714 g          852 t
ORIGIN      
        1 gaaaatccac ctacagcgcc aatcatctga atatcatcca gggtttcacg tccactgatc
       61 aaatcggtca tgtgcacatc cttcacatca aatccggctg agtaaagtgc attggccagc
      121 tcccgttccg aattactccc cttttcccgg agaacagcag cctttgggcg tggcctggca
      181 gaatcaatgt gtggcaattt tccatcaaaa gaagccggga atttataatg caacaaatga
      241 tgcttatagt tgtcatatcg ctcctttgcc ttcttcggtc cactttgtct gatatccagc
      301 agataggatg tttcgaacca gatgtcacgc aatctgtcga tatcaaaagt gtgagaatct
      361 tcatgatccc tgacctgcaa agtggcttct cttcttattt tgcctattgg gaaaaattca
      421 actccggcac caatcaatat ttcttcagca actgaggatg cctgtatgac tactcctgca
      481 ttttctgcaa acaatttatg gatgatgtct tcaccgggaa gcgctgaaag gtcgatttca
      541 gcccctagtc cacggtcagc gaagcacatt tccagcaagg tggtaatcaa gcctccgctt
      601 ccaatgtcat ggcccgcaga aatcaacccc tgactgacca gactctgaat aacagcaaat
      661 ttttcaacaa attcggatgc atctgcaatg tccggagcgg tatctcctac tctgttcaat
      721 acctgagcga atgaagaacc tcccagccgg ggtgacattc cggaaaaatt gatataatag
      781 atcccggcat cctctttttt caggacgggt ccaacgacct ttctcacatc attacaatgt
      841 ccggctgctg aaataatgac agtaccgggt gccaagacct ctccttcggg atatttctgc
      901 ttcatagaga gagagtcttt tccggtaggt atgttgattc ccaaggcgat ggcaaagtcc
      961 gaacaagccc tgactgcgtt gtacagtcgt gcgtcctcgc cttcattctt acaaggccac
     1021 atccaattgg cagagagcga tatgcttcgg atgccatcct gcagcggggc ccagacaata
     1081 ttggtcaatg cttcggcgat ggcatttctg ctccctgcag cagaatcaat cagtgctgtc
     1141 agtggtgaat gccccaccga tgtggcaatc ccgtcacggc cggaataatc cagtgccatc
     1201 actccacaat tgtttagcgg tagctgaagt ggtccggcac attgctggac tgccaccaag
     1261 cctccgacac agcgatccac cttattggtc agccagtcct tacacgccac ggcctcaaga
     1321 cgcagcacac tctccagata ttcgtcaatt cttttcctgt catatatgag gggtgaatat
     1381 tgcctgtcga tggtcttgtc ttccatgatg gtctttggtg agctgccgaa catgtcacca
     1441 agtttcagat ccatcggtct tgcaccggta gatgaggaag ctatggtaaa gcgatgatca
     1501 ttgctgactt tgcctacaat atacatggga gctctttctc gttcggcaac tttgttgagg
     1561 gtgtcgatat gactgtcagc aatcaccagg cccattcgtt cctgtgattc gttgccaatg
     1621 atttcacgcg cagatagggt tggatctcca acaggcagtt tatcaaggtc gataaatcct
     1681 ccggtttgtt caaccagttc agacaagcag ttgagatgac cgcctgctcc atgatcatgt
     1741 atggacagga tgtgattatg atcgctttcc accatacctc ggatggcgtt ggctactcgt
     1801 ttctgcattt cagggttaga ccgctgcaca gcattaagtt ctatgccgga gccaaattct
     1861 cctgtatcag cggaggagac agccgctccg cccataccaa tacggtagtt ttcgccaccc
     1921 atgacgatga tcttatcacc tgccgccgga atgtgctttt ttgcctgtcc cgccttccca
     1981 aaaccaatgc ctccggcttg catgataacc ttgtcataac caagggtacg atctgcttcc
     2041 ttgtgttcaa atgttagcac ggaaccacag attaggggtt gtccgaactt gtttccaaag
     2101 tcagaggcgc cattggaagc cttgatcaga atatccagtg gtgtctggta aagccatttt
     2161 cgctcggggt tagccttttc ccaatcccta ttatcactca gccgtgagta gcttgtcatg
     2221 tataccgcag tacctgccag aggcaaggct ccctgtccac cggcaagcct gtcgcggatc
     2281 tcccctccgg agcctgttgc cgcgccattg aatggttcga ctgtagtggg aaaattatgc
     2341 gtctccgcct tgagagatag gacagaagcc acttccttta tttcaaaata ctccggaaca
     2401 tcgggtcttt tgggagcgaa ttgcctgatg attggtccct tcagaaaggc tacgttgtcc
     2461 ttgtatgcag agacaatgga gtttggattg gcctgtgatg tcttcttgat catccggaaa
     2521 agcgagtccg gctgctcctc gccatctatg acaaaggttc cattgaagat tttgtggcgg
     2581 caatgttcag agttgacctg agagaatcca aacacttctg aatctgtgag cggcctgcca
     2641 agtctatctg aaaggtcatt gaggtacaga atttcctctt cactgagggc cagaccttct
     2701 gtctcattgt aggtggcaat atctgtaatt tcaagaattg gttccggctt gctcctaacg
     2761 gaatatatgt cttgacccaa ggaattgtat ttggctgaaa gcatgggatc ataggaagga
     2821 tgatcacttt ctgacctgac aaattcctca atacgggtga ttccttcgat ggccatattc
     2881 tgcgtaatct caaccgcatt ggtactccat ggtgtcacca ttgtggctcg aggcccgaca
     2941 aaggtacctt ccacaacctt gctctctatc aatctgccat cgccaaaaag ccaggagagt
     3001 ttggatatgt cgctgtcgga aagggcattc tctgtttgta gtgcatagac agtattgcca
     3061 ctgccttcaa agaaaaaaat cattttcagc catttaagtc gcaaaggtac tccgaaaaaa
     3121 ggtagtaaga aaat
//
LOCUS       DAOQQN010000363         3114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_16006_length_3114_cov_6.229814, whole genome shotgun sequence.
ACCESSION   DAOQQN010000363 DAOQQN010000000
VERSION     DAOQQN010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16006_length_3114_cov_6.229814"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..623)
                     /locus_tag="PLG24_11585"
     CDS             complement(174..623)
                     /locus_tag="PLG24_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25580.1"
                     /translation="MKIKWIYIIALLLMAPGYAWSQKLSSGEVPSIVLNNFKLAYPKA
                     YDVSWKKSGESYSAEFCVEKKCNLKVWFNKYAQLTKEMEELVFNTVPDRIKTKVKENY
                     GKFKIDEITKETVGKTTKYILKLESKFEDLKIIFNEDGKELSKEEDD"
     gene            complement(978..2246)
                     /locus_tag="PLG24_11590"
     CDS             complement(978..2246)
                     /locus_tag="PLG24_11590"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMZ25581.1"
                     /translation="MKLFAKTFRYLIVIQLIIVTLWSVAFYYSMMHEIYDSIDDGLDN
                     YKLLIIKKAASDTSILYKSQFDESNYAIRAIPFTQAVSITDRYRDTLLYMPFEKKLEP
                     VRILHTAFENNGKYYELKIISSMVEEDDLIKSLFWAVVILFCVLNITILSINHFVLKR
                     IWKPFYKYLKRLNLFKIESDPDILPMKTDVSEFVQLNQSVDMLVKNSRKSYSNLKSFT
                     ENASHEIQTPLTSIIHHLELMLADSKLNKDHAQSAIKAHQSALRLSKINKALLLLTKL
                     DNQQFQNRGIIDFEIIFNNVIDELSELAEFQGKTIEMEISGKFKHTMDHELASILVVN
                     LVKNAIIHSLRGTHITIRIFDQQFSVENEGAHGPLPDEQIFTHFFKTENSAPGLGLGL
                     AIVQSICRHEQFKIEYSFEGKHKFTVTGTK"
     gene            complement(2243..2920)
                     /locus_tag="PLG24_11595"
     CDS             complement(2243..2920)
                     /locus_tag="PLG24_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ25582.1"
                     /translation="MKILLIEDDESLASAIREYLKSEDILVEVAQNFQKGQEKAMLYE
                     YDCILLDLTLPGGDGMEILKTINNSPVKNNVIIISARGALEDKITGLESGADDFLPKP
                     FHLAELNARIKAVVRRKLGSNSNEIRIGEIRIDLNNRKVYIGQQEIQLFRKEFDLLYY
                     FAINKNRLLTKSAIAEYVWGDHYEDADNLDFVYYQIKNLRKKLLEMNAPFQIASSYGI
                     GYIFKDL"
BASE COUNT          912 a          561 c          557 g         1084 t
ORIGIN      
        1 atcgagcaat gtctccttgt catcacagcc aatcagcgaa atggttgaga tcataaaagc
       61 taaaaacaaa atctttttca tgttgattta attatttggt ttaaaaatta atgtttttta
      121 tgatatataa ctgaatatga tataatattt taataggtaa tggtattaag taatcaatca
      181 tcctcttctt ttgataattc ttttccatct tcattgaaaa tgatttttaa gtcttcaaat
      241 ttagattcaa gtttcaggat gtattttgtt gtcttgccga cagtttcctt tgtgatttca
      301 tcaattttaa acttgccata attttctttg acttttgtct tgattctatc tgggacggta
      361 ttaaatacca gctcttccat ttccttggtc aactgagcat atttgttgaa ccagaccttt
      421 aaattgcatt ttttttcaac acaaaactcg gcactataag attctcctga ttttttccaa
      481 ctgacatcat aagccttggg atatgctaat ttaaaattgt tgagtacaat ggatggtacc
      541 tcacctgaac tgagtttttg actccatgca tacccgggtg ccatcagcaa aagggcaata
      601 atgtaaatcc attttatttt catctgattc ataaattcat tgcaaagtaa atccgtctct
      661 ttggaaaaaa atgggaatca tacctgattg aataattttc ccggatgtca ttccttggag
      721 taaaccatcg gtatgtttta ccaagatgat ttataaaatt aaccaaaaat attcggtatt
      781 attttcatta ttaccatttt gaatggacaa cagtatcagg cccttatttt tttataacca
      841 taggggataa catgtaggaa tgctgtcgga aagcgattgg gttgtcaccg aagtataata
      901 gggtagtttg atacatccta ttagacatca taggagtcaa ttccggaagg aagtttttca
      961 ataggtatct cggtgattca tttagttcct gtaaccgtaa atttgtgttt cccctcaaaa
     1021 ctatactcaa ttttaaattg ctcatgtctg catattgatt gtactattgc taatccaagt
     1081 ccgaggccgg gagctgaatt ttctgttttg aaaaaatgag taaatatttg ctcatcaggc
     1141 agtggcccat gtgctccttc attttccact gagaattgtt ggtcgaaaat ccggatggtt
     1201 atatgagttc cacgtagtga atgaataatg gcatttttga ccagattgac aaccagaatt
     1261 gatgccagct cgtgatccat ggtgtgtttg aattttccgg aaatttccat ttctattgtc
     1321 ttgccctgaa actctgccaa ttctgataat tcatcaatca cattgttaaa aataatttca
     1381 aaatcaatga tacctctgtt ttggaactgt tggttgtcaa gttttgtaag caacaagagt
     1441 gctttgttga tctttgagag ccgaagtgcc gattgatgag cttttattgc cgattgtgca
     1501 tggtccttat tcagcttact gtcggccaac atcaattcca gatggtgaat aatcgatgtg
     1561 agcggggttt gtatttcgtg agatgcgttt tcagtaaagc tttttaaatt agaataactt
     1621 ttgcgggaat tttttaccag catatccacg gattgattaa gctggacaaa ttcactgaca
     1681 tcggttttca tgggtagtat atcgggatct gattctattt tgaacaaatt tagccgtttt
     1741 aagtatttgt agaatggctt ccagattctt ttcaatacaa aatggttgat acttaagatt
     1801 gttatattga ggacgcagaa caaaattaca accgcccaaa acagactttt aatcaggtca
     1861 tcttcctcta ccatagagga aatgatcttt agttcatagt attttccatt attttcaaat
     1921 gctgtatgta ggattcgtac cggttccagt ttcttttcaa aaggcatgta aaggagggtg
     1981 tcccgatatc tgtcggtgat ggatacggct tgtgtaaacg gaattgcccg gatggcataa
     2041 ttactttcat caaattggga cttgtataat atggatgtgt cacttgctgc ttttttgatg
     2101 atcagaagtt tgtagttgtc cagcccatca tcaatgctat catagatttc atgcatcatg
     2161 ctataataga aagcaacaga ccaaagcgtc acgatgataa gctgaatgac gatcaggtag
     2221 cggaaagttt tggcgaaaag tttcataaat ctttaaaaat gtatcctata ccgtaagagg
     2281 aggcaatctg gaatggtgca ttcatttcaa gtaatttttt tctcaaattt ttaatttgat
     2341 aatatacgaa atcaagattg tcggcatctt catagtgatc gccccataca tattctgcaa
     2401 tggcactctt ggtcaataac ctgtttttat tgatggcaaa ataatataac aggtcgaatt
     2461 cttttctgaa aagttgtatt tcctgctgcc caatatagac tttgcgatta ttcagatcga
     2521 tccttatttc accgatcctt atttcatttg aattactccc taattttctt ctgacgaccg
     2581 ccttgatacg agcattaagt tctgccagat gaaatggctt aggtaaaaaa tcatctgcac
     2641 ctgactccag accggtaatt ttgtcttcaa gagctccccg ggctgagatg atgataacat
     2701 tgttttttac gggggaatta ttgattgttt taagtatttc cataccatca cctcctggca
     2761 atgttaaatc gagcaagata cagtcatatt cgtataacat cgctttttcc tgtcctttct
     2821 ggaagttttg tgcaacttca acaaggatat cctcactttt cagatattcc cggatcgcac
     2881 tggccagcga ttcatcatct tctatcagaa gtattttcat caggcaaaat tatagattaa
     2941 aactggaaca aaccgggaat gatacaacca tccactattg agcataaaat atgagtcatt
     3001 caactcattc aaatattcct gactcaatca atgcgccacc ctggtataag cgccgtaaat
     3061 catgagaaaa taacttcccg gtaaagctta gaattttgca tgattcaaat attt
//
LOCUS       DAOQQN010000364         3100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_16122_length_3100_cov_4.649918, whole genome shotgun sequence.
ACCESSION   DAOQQN010000364 DAOQQN010000000
VERSION     DAOQQN010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16122_length_3100_cov_4.649918"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PLG24_11600"
     CDS             complement(<1..897)
                     /locus_tag="PLG24_11600"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HMZ25583.1"
                     /translation="MKKYFSPGLSIMFVVTAVCYVLFYLRPDLAFRVQTNGVFALIRR
                     LYTGMSSILVTLLWILIFISYFKYRHSRGAPPSLFRWIKYLFEPVLWIFTLYFWLWGF
                     NYLAPRPEHFLLKDTSAPDSRELLEAFHFHTNLVNTLRDSLDLKKEITVRDILTTHGE
                     HWQRQTVSTLAQAGYNGSGKPEIQVVRPEGLLLRISTAGFYNFVLARPTIDPGLHELQ
                     VPNTALHELAHAYGVASEASASFVAYIVGIQSEDPLTRYSVELALWRTLRNTCFMVDS
                     AATKTISGFVNPEVKKDIKNIRM"
     gene            972..1289
                     /gene="trxA"
                     /locus_tag="PLG24_11605"
     CDS             972..1289
                     /gene="trxA"
                     /locus_tag="PLG24_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HMZ25584.1"
                     /translation="MAFEFTDSNFQEKALGNDGLKVVDFWAEWCGPCKAIGPIIEELS
                     KEYEGKATIGKLNVDDNPEVSFKYGIRSIPTLLFIKNGEVVEKHVGAASRQVIASKIE
                     ANL"
     gene            1448..2377
                     /locus_tag="PLG24_11610"
     CDS             1448..2377
                     /locus_tag="PLG24_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HMZ25585.1"
                     /translation="MSFFDTIDLKQHTNLELIARKVVEGFIIGLHKSPFHGFSVEFAE
                     HRLYQPGESIRNIDWKVYARSDKFFTKKYEEETNLRARIVIDISSSMHFPGKEVYNEV
                     RMNKLQWSALAAAALSYLLKKQRDAFGLTLFDEQPVYHSEAKSSSAHQQLIFSQLEPY
                     IYDQTFNKKTSAADSLHRIADMIHKRSLVIIFSDMLEDPARMEPMFDSLQHLKHNKHE
                     VILFHVVDASRELLFEFDNRPYEFIDLESGEKLRLQSNEIKREYADKMKQYEEELRLK
                     ALQYKIDFVSADINRDFDQVLQAYLIKRSRMRT"
     gene            complement(2746..>3100)
                     /locus_tag="PLG24_11615"
     CDS             complement(2746..>3100)
                     /locus_tag="PLG24_11615"
                     /inference="COORDINATES: protein motif:HMM:NF025028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DKNYY domain-containing protein"
                     /protein_id="HMZ25586.1"
                     /translation="YAKDAGHVYFRDAIIPGAEPSSFTTMQFPYSKDRKDVYCGNIPL
                     RLSPEDVNTFKVTNEDKMMAGSISTMKLEHFLKYNPDYSWLAEMKDTMEMVITGESGT
                     AVSQSKKFKGYKEVK"
BASE COUNT          947 a          694 c          687 g          772 t
ORIGIN      
        1 catccgtata ttttttatat ctttctttac ttcaggattg acaaatcccg atattgtttt
       61 tgttgcagca ctatccacca taaaacaagt attacgaagg gttcgccaca aagccaattc
      121 tacagagtat cgggtcagcg gatcttcact ttgaattccg actatatacg cgacaaaact
      181 ggcacttgcc tcacttgcaa caccgtaggc atgcgccaat tcgtgcagtg cggtgttcgg
      241 cacctgaagc tcatgcaatc cagggtcgat ggtaggcctt gccagaacga agttgtaaaa
      301 cccggcagtg ctgatccgca ataaaaggcc ctccggcctg accacctgaa tctcaggctt
      361 accactgcca ttataaccgg cctgagcaag tgtcgacacc gtttgcctct gccaatgttc
      421 accatgcgtt gtcaggatgt ctcggactgt aatttccttt ttcaaatcca gggagtcccg
      481 tagcgtattg acaagattgg tatgaaaatg aaaggcctcc agcaactcac ggctatcagg
      541 agcggaagta tctttcaaca ggaaatgttc cggccttgga gccagataat tgaaccccca
      601 taaccagaaa taaagtgtga aaatccaaag tacaggctca aacagatatt taatccaacg
      661 gaaaagggat ggtggagctc cacgactatg ccggtatttg aaatagctaa taaagatgag
      721 aatccataaa agagtcacca agatggatga catgccggta tataaccttc gtatgagggc
      781 aaaaacacca ttggtctgta cccggaatgc aaggtcagga cgcaggtaaa aaagtacgta
      841 acacaccgcg gtcacaacaa acataatgga aagaccgggg gaaaagtatt ttttcatgtt
      901 ccgatgaacc ttttttagaa aaatatgttt gtacaaaagt attcaaaaca ttaataaaac
      961 aagtttaaaa aatggcattc gaatttacag acagtaattt tcaggagaaa gcactgggta
     1021 atgacggttt gaaagtggtt gatttttggg cagaatggtg tggtccttgt aaggccatcg
     1081 gtccgatcat cgaagagctc tcaaaggaat atgagggaaa ggcaaccatc ggcaagctca
     1141 atgtagatga taaccctgag gtttctttca agtatggcat tcgcagtatc cccaccctgc
     1201 tgtttatcaa gaatggagag gtggtggaga agcatgttgg cgccgcttcc cgacaggtga
     1261 ttgcttccaa aatagaagcc aacctataaa attcggctta gccagaagaa atattccctt
     1321 acgcagcaac gtttgtgtaa gggttttttt attggaaggc gccggaacag cgatatgatg
     1381 tatagatacc ccatgatggg agacctctgt acaccccaaa atactgacaa cacataacac
     1441 atcgaccatg tccttttttg ataccattga cctgaagcaa cataccaacc ttgagctgat
     1501 agcccgtaag gtagtggaag gctttatcat cggcctgcat aagagcccgt ttcacggctt
     1561 ttctgtggag tttgccgaac acaggctgta tcagccggga gaaagtatcc gcaatatcga
     1621 ctggaaggtg tatgcaaggt cagacaagtt ctttaccaag aaatatgaag aggaaaccaa
     1681 ccttcgcgcg cggattgtca tcgatatatc ctcctccatg catttccccg gtaaagaggt
     1741 atacaacgaa gtgcggatga ataagttaca atggtcggct cttgccgccg ctgcactgtc
     1801 ctacctcttg aaaaagcaac gcgacgcctt tggtctcacc ctttttgacg aacaaccggt
     1861 gtatcattcg gaggccaagt ccagcagtgc acaccaacag cttattttct cccagcttga
     1921 gccatatatt tatgatcaga catttaacaa gaagacgtct gctgcggact ctctgcaccg
     1981 gatagcagat atgatccaca agcggtcact ggtcatcatc ttcagcgaca tgctggaaga
     2041 cccggcacgg atggagccga tgtttgattc actacagcat cttaaacata ataagcatga
     2101 ggtaatcctc ttccacgtgg tggatgcctc ccgcgaattg ttgtttgagt ttgataaccg
     2161 cccctatgag ttcatcgacc tggaaagtgg cgaaaaacta cgcttgcagt ccaacgagat
     2221 caaaagggaa tatgccgaca agatgaaaca atatgaggag gaactacgtc tcaaggccct
     2281 gcaatacaaa attgactttg tatcggccga tatcaaccgc gattttgacc aggtgcttca
     2341 ggcgtatttg atcaagcgga gcagaatgag gacataaaag agacaaggat acacgaagct
     2401 tagaggacga gatagggcgc aggacgattt tcaaaggacc caaggaaaaa cgccaacaat
     2461 cagaagagtc cttaacaaaa cagaaaagga aattatatca atttaggtca aggcaatccc
     2521 tttactcacg tgcatgatat acatcattgg cattgtttaa gagagaacac acaatgagga
     2581 gtcatcagcg acttgtaaaa cttagcaata cagactgata cttccgggca ggcaccctcg
     2641 tcagtatggt cagcaactgt ggcaagagaa gataaagccg aaatgacttc atgcttttct
     2701 gccatgtaaa tataggcgct aaaagataag tgccggagaa atcatctact tgacttcttt
     2761 atatccctta aatttctttg actgtgaaac tgcagtgccg gactccccgg taataaccat
     2821 ctccatggta tccttcattt cagccaacca ggaataatca ggattatatt ttaaaaagtg
     2881 ttccaatttc atagttgaaa tagaacctgc catcatttta tcttcattgg taaccttaaa
     2941 ggtattgaca tcctccgggg ataatcttaa gggaatattt ccacaataaa catcctttct
     3001 atctttcgaa taagggaact gcatggttgt aaaactcgaa ggttccgcac cgggaatgat
     3061 tgcatcgcga aaatatacat gcccggcatc ctttgcatag
//
LOCUS       DAOQQN010000365         3081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_16296_length_3081_cov_5.739260, whole genome shotgun sequence.
ACCESSION   DAOQQN010000365 DAOQQN010000000
VERSION     DAOQQN010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16296_length_3081_cov_5.739260"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PLG24_11620"
     CDS             complement(<1..455)
                     /locus_tag="PLG24_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25587.1"
                     /translation="MKKTKFTARFRSERFFNPLMGILLFCSLFFYQERLNAQCSPQCE
                     PSNISLDADCIARVNFAMVVNPLSLAGCGPVFTLNLRATMNGPVLETVNNGTLVVDGM
                     SIGGQPYLYKGKTMVLEYVSVSTGNSCWNWHTFEDKLPPVIDCGATTIIP"
     gene            complement(1001..>3081)
                     /locus_tag="PLG24_11625"
     CDS             complement(1001..>3081)
                     /locus_tag="PLG24_11625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ25588.1"
                     /translation="PDSSVVIECTQWLLNGYTITAPFVDTISACFNASISYVDSVIVP
                     SFPGSVLAIKRVWQVKDDCFYDPNTGIGIFYFNQIIDVIDTTGPIVINYVDGDTINAP
                     LYTDDCKILLDLSDLQILDCSPIVVQTHSINGGTPANNIDASGYYTLGVSTVNYYFED
                     IWGNGTNFTLYIQVLDSFPPTWPCPESLDVYINPNGLDTVYASQFSFQPFQAFDNCNY
                     HDIYMTFDSTNLADSVLYLVCTGNVSDLSIQNLKVWVFDDVGLSTSCVVDLNVLEPPG
                     QTTNCNHLMGVVGQLAADNGHPIPGAKVSAKGTVEVNQMSDAFGRYEIHDIPPGSDLS
                     MSVEKHESAINGVNVSDIIAIRDHILAKKPLNNPYKLLAADVNDDGYVSVRDLLFIRK
                     LILGYIDQYPSGLSWRFADKSYKFPNKKNPFNQPDVWQRQLHDVKGVLPVVDWVGIKL
                     GDVNDSALFGAEVVETREVKPVNVVVTKVDESETEVTIRLIMDEIEDIQGIQFDMQYD
                     IADLELTSLQLGDLSGITSDHFRVNEYTGSVSFVWDDEAGRNGNVIATLTFRKTGQGP
                     VGNILAINDERIEAMAIDPDGKEHKVNLVSTGLWSDKENKEGRLLQNSPNPFNNSTVI
                     SVILAQSVKGKLTVYDATGRKVYTKTGEFSKGRNTIEIKAGNLSGSGIYIYRFESEIF
                     TDTKKMILTL"
BASE COUNT          941 a          679 c          552 g          909 t
ORIGIN      
        1 ggtataatag ttgtagctcc acagtcaatc actggtggca acttgtcttc gaacgtatgc
       61 cagttccagc aactatttcc agtggaaaca ctaacgtatt ccagaaccat agtcttacct
      121 ttatacaaat aaggttgacc tccgattgac ataccatcca caaccaaagt accgttgttt
      181 actgtttcaa gtacagggcc attcatggtg gctctcaggt tcaatgtaaa cacaggacca
      241 catccagcca gtgataaagg attaacaacc attgcaaagt taacacgggc aatacaatct
      301 gcatccaacg agatattgga tggctcacac tgcggagaac actgtgcatt caggcgctcc
      361 tggtaaaaaa atagtgaaca gaaaagcaat atacccataa gcgggttaaa aaacctttcc
      421 gacctaaaac gagctgtaaa tttcgttttt ttcatgagac acttagttta agtttttaga
      481 attaagaatt aaaatatggt gcatgccaca ccccagcgtt ctgggagcgc aatactgtag
      541 tttcttatta taattcacac ccgatgtgta tcgacacaac ggatgtcatt gtcgttcgat
      601 caatttgatt aaaccacttt tataaatggt cgaatcatgt tcatcgacat aacaaatcat
      661 ctctccggaa atatttcatt ccgaaaaaag ttctaattct aaattatctg gttggaaaag
      721 gttataagct caaatgttac atttgacctt ttgtaattat atctgatagt tgtaggttgg
      781 ttcggttctt ataaatcaga tatagttaca gattttgttt ttacgtattt ctttcaatac
      841 atggcacaaa tatataatat gtacgtttgt ctgaaccata ccaacatagt gctatattat
      901 atattaaata ttcaattcat ttataaatac ctgatttaca aaatataaaa aggggagaaa
      961 attctccccc cttttaaaat atggtcttaa ttcaaaaaaa ctacagtgta agtatcattt
     1021 tcttggtatc ggtaaatatt tccgactcaa atcgatagat gtatattccc gaacctgaca
     1081 ggttcccggc tttgatctca atcgtatttc tacctttact gaattctccg gtttttgtat
     1141 ataccttcct gccagtcgca tcatatacag tgagcttacc ttttacagac tgtgccaata
     1201 tcactgaaat tacagtggaa ttattgaatg gattcgggct gttttggagc aaccttcctt
     1261 ctttattctc cttatcactc caaaggcctg tggaaaccag gttgacttta tgttccttgc
     1321 catcaggatc tattgccatt gcctcaattc tttcgtcgtt tattgccaga atatttccta
     1381 ccggaccctg accggttttc ctgaaggtca aggtagcaat cacattgcca ttcctgcctg
     1441 cttcatcatc ccagacaaat gaaacggagc ctgtatattc attgactctg aaatgatccg
     1501 aagtaatccc cgacaagtcc cctaattgaa ggctggtcaa ttcaaggtcg gcaatatcat
     1561 actgcatatc gaattggata ccctgaatat cctctatctc atccattatc aatcttatgg
     1621 ttacctcagt ttccgattca tccacttttg tgacaaccac attaacaggc ttaacttcac
     1681 gtgtttcaac tacttcggca ccaaagagcg ccgagtcatt gacatctccc agcttgattc
     1741 ccacccaatc aacaacaggc aatactccct tgacatcatg caattgcctc tgccagacat
     1801 caggctggtt gaaaggattt ttcttatttg ggaatttgta agatttatcg gcaaacctcc
     1861 aactcaatcc ggatggatac tgatcgatat atccaaggat cagctttctg ataaacaaga
     1921 gatcccttac acttacatat ccatcatcat tgacatcggc cgccagaagc ttgtatggat
     1981 tgttcagtgg ctttttcgcc aggatatgat ccctgatagc aatgatatcg ctgacattga
     2041 ctccattaat cgcactctca tgcttttcaa cactcatact taaatcagag ccgggaggaa
     2101 tatcatgtat ctcgtatcgt ccaaaggcat ccgacatctg attgacttca acggtccctt
     2161 tggctgatac ctttgctccg ggtataggat ggccattgtc tgcagccaat tgtcccacta
     2221 ctcccattag atggttacag ttggtagtct gtccgggtgg ttcgagcaca ttcagatcaa
     2281 caacacaaga agtactcaat cccacatcat cgaagaccca caccttcaga ttttgaatgc
     2341 tgaggtcact gacatttcct gtacagacaa ggtacaagac agaatcagcc aaattcgtgg
     2401 aatcaaatgt catatagata tcatgatagt tgcaattgtc aaaagcctgg aagggttgaa
     2461 aggaaaattg actggcataa accgtatcca aaccattagg attgatatac acatctaagc
     2521 tttcgggaca tggccaagta ggcgggaagg aatccaatac ctgaatatac agtgtaaaat
     2581 tggttccatt gccccagata tcttcaaaat aataattcac tgttgagacg cccaatgtat
     2641 aataaccgga tgcatcaata ttattggctg gtgtaccgcc attgatagaa tgcgtctgga
     2701 cgacaatagg cgaacaatca agtatttgaa gatcactcaa gtcaagaagt attttgcaat
     2761 catccgtata taatggtgca ttgattgtat caccgtcaac ataattgatt actataggtc
     2821 cggtagtatc tattacatca atgatctgat tgaagtagaa tatgccgatt ccggtgttgg
     2881 gatcatagaa acaatcatcc ttgacctgcc aaaccctttt aatagcaagg acactaccag
     2941 gaaaactagg tacaatgacg gaatccacat atgaaatgga ggcattgaaa caagccgaaa
     3001 tggtatccac aaaaggtgca gtaatagtat agccattcaa caaccattga gtacactcaa
     3061 tgacaacgct tgaatccggc c
//
LOCUS       DAOQQN010000366         3035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_16711_length_3035_cov_4.554698, whole genome shotgun sequence.
ACCESSION   DAOQQN010000366 DAOQQN010000000
VERSION     DAOQQN010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16711_length_3035_cov_4.554698"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1749
                     /locus_tag="PLG24_11630"
     CDS             67..1749
                     /locus_tag="PLG24_11630"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25589.1"
                     /translation="MKTGGSDAALSATGGFADSGRMTIVDFQWPSMAAVGVAWQATSA
                     VLLAADVKRIGWSDVMQSFRMRYDSAGIGGSVSFALPQNWKDQTVTNLGKFTQPQMYD
                     IIAKHPNPREIYLKQLLSRGDINKEEAQRLENVYWSNLQARLDDVKQKPLPYTYQEPE
                     LAWKALRKTFDPKDFLVSPKTGIKKATLEKILKHLQSLPEGFTPLSKVNRLLKSKDEL
                     LSSGSMDWALAELSAYGSILLEGKDVRMSGQDVRRGTFSHRHATFRDAETFDTYNRLT
                     GLSEDQGRFMIYNSLLSEFAVLGFEYGYSLANPNSLVLWEAQFGDFANGAQTIMDQFI
                     TSGETKWQRMSGLVLLLPHGYEGQGPEHSSARLERFLSMCADYNVTVANLTTPANFFH
                     VLRRQLARPFRKPLIVMSPKSLLRHPECVSSINDFVTGNGFREVYDESELTDIKSDKF
                     TKIICCTGKIYYDLLAYRRSNKIKDAAIVRVEQLYPYPKNQIDEILGKYKKARIRWVQ
                     EEPANMGALTYIRTLMPEGTEFIARKASASTATGFKKTHDKQQAELMVKAFE"
     gene            2318..>3035
                     /locus_tag="PLG24_11635"
     CDS             2318..>3035
                     /locus_tag="PLG24_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HMZ25590.1"
                     /translation="MSNNPKNIRNIVLLGHSGSGKTTLVENMLFEAKKINRIGSVDDN
                     STTSDYIEIEKERHSSVYSSLMHVNWKDSKINIIDTPGSDDLVGESISSMKVADTAVM
                     LINAKYGVEVGTELIWEYIEKFHLPSVFAVNHLDNSQADFERALAQARERFGSKVVEV
                     QYPLDAGEGFSSIVDALRMVMYKFPSNGGKPSKEKIPDAEMERAKSMHNTLVELAAEN
                     EEGLMERYFEEGTLSEEDLAK"
BASE COUNT          888 a          686 c          713 g          748 t
ORIGIN      
        1 gtctggcgcg ccgcgcccgg cgtcacgctc ggcgcgtcgt tccagggcaa gacctcgctc
       61 ggcgacatga agaccggcgg cagcgatgcc gcgctgagcg ccaccggcgg cttcgccgac
      121 agcggccgca tgaccatcgt cgacttccag tggccgtcga tggcggcggt cggcgtcgcc
      181 tggcaggcca cgagtgccgt gctgctggcc gccgacgtga agcgcatcgg ctggtccgac
      241 gtcatgcaga gcttccggat gcgctacgac agcgccggca tcggtggctc ggtcagcttc
      301 gcgctgccgc agaactggaa ggaccagacg gtcacgaacc tcggtaaatt tacccagcct
      361 cagatgtacg acatcatcgc aaagcatcct aatccaagag aaatctatct gaagcagttg
      421 ttgagtcgtg gtgatatcaa taaggaagaa gctcagaggc ttgagaatgt atattggtcc
      481 aacctgcagg ccagactaga cgatgtaaag caaaaaccgc ttccctatac atatcaggag
      541 cctgaactgg cctggaaagc actgagaaag acctttgatc caaaggattt ccttgtatct
      601 ccaaagacag gaataaaaaa agcaacctta gaaaagatat tgaagcatct gcagagtctt
      661 cccgagggct ttacccctct gagtaaggtc aacagactct taaaaagcaa agacgaactg
      721 ctctcttccg gctcaatgga ctgggcgctg gctgagttat cggcctatgg ttcgatcttg
      781 cttgaaggga aggatgtcag gatgagtggc caggatgtac ggagggggac cttctctcat
      841 cgtcacgcaa ccttccgtga tgctgagact tttgatacct acaacaggtt gacaggcctg
      901 tctgaagatc agggtagatt catgatatac aactccctac tatcagaatt tgctgtactt
      961 ggctttgaat acggatattc actggccaat ccgaacagcc tggtcctgtg ggaagcacaa
     1021 tttggtgact ttgccaatgg cgctcagaca atcatggacc aattcatcac ttcaggtgaa
     1081 acaaaatggc aaaggatgag cggcctcgta ctccttctgc cacatggata tgaaggccag
     1141 ggaccggagc atagtagcgc aagactcgaa agatttctca gtatgtgtgc ggattacaat
     1201 gttaccgtag ccaatctgac cacccctgct aacttcttcc acgttttaag acgtcagttg
     1261 gccagaccat tccgcaaacc attgattgtg atgtctccaa aatcattgct cagacatccc
     1321 gagtgcgtat catccatcaa tgattttgtt accggaaacg gcttcaggga agtatatgat
     1381 gaaagcgaac tgactgatat caaatccgat aagtttacca aaatcatctg ctgtacagga
     1441 aaaatctatt atgatttgct tgcctacaga agaagcaata agatcaagga tgctgccatc
     1501 gtgcgtgtag aacagctcta tccatatccg aaaaaccaga tcgatgagat attgggcaaa
     1561 tataaaaaag cccggataag atgggttcag gaagaacctg ccaatatggg tgccctcacc
     1621 tatatcagga ccttgatgcc ggaaggaacg gaatttattg ccagaaaagc gagcgcatct
     1681 acagcaacag gatttaagaa gacccatgac aagcaacaag cggagttgat ggtcaaggct
     1741 tttgaataaa aagatgataa taaaattcag agcggagcag tgcagagatt aattcctgcc
     1801 tgctccgctt ttttattatc ccaaagagac ccaacggatt ttgttatcag gccgggatat
     1861 gattcctgac attacaaaag acctattcgt atcacttctt ctattccttg gcacaataaa
     1921 aagtccttcc acttaggctg cttgtcagtg gttcctatcc tttgctatta taagtggcga
     1981 accttcaaaa accaaccgaa tatttcaatg cccaaacaca ccctgcgaga aatatcagcc
     2041 ggcaattaat ttgccttcgc aattgaatat tttacaataa aatttttcaa tacgaagtaa
     2101 aatataagcc tctccaaatt tcattccata caatgcctct ataattggct tttattccaa
     2161 acaattaact ttaaaattat tttttctcaa aattttcacc aatttaaaat tcaaatgatt
     2221 acttttagct aaaattggcc aggtgcaatt ttgcattact acaaaaagaa tatgcgcacc
     2281 ggcttttact tgttttactt aaatataata tctgtctatg tcaaacaatc ctaaaaacat
     2341 tagaaacatt gtcctgcttg gacactcggg tagcgggaag actaccttgg ttgagaatat
     2401 gcttttcgaa gcgaagaaaa tcaacaggat aggttcagtc gatgacaact cgacgacttc
     2461 cgattatatc gaaatagaaa aggaaaggca tagttcggta tattcttccc tgatgcatgt
     2521 aaactggaaa gacagtaaaa tcaacatcat cgacacaccc ggatcggatg atttggtcgg
     2581 cgagagtatt tcctcaatga aggtagctga tactgctgtc atgttgatta atgccaaata
     2641 tggggtcgaa gtgggaaccg agctgatctg ggagtatatt gaaaaattcc acctgccttc
     2701 agttttcgca gtcaaccatc tggacaattc tcaggctgac tttgagagag ctctggctca
     2761 ggccagggag aggtttggaa gcaaggttgt tgaagtacaa tacccactgg atgcaggtga
     2821 gggattttcc agtattgtag acgcattgcg tatggtgatg tataagtttc cgtccaatgg
     2881 aggcaaaccc tccaaggaaa aaatcccgga tgcggaaatg gaacgtgcca aatcgatgca
     2941 taataccttg gtggaactgg cagcagaaaa tgaggaaggc ttaatggagc gatattttga
     3001 ggaaggtacc ctgagtgaag aggatctggc gaaag
//
LOCUS       DAOQQN010000367         3027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_16780_length_3027_cov_5.524899, whole genome shotgun sequence.
ACCESSION   DAOQQN010000367 DAOQQN010000000
VERSION     DAOQQN010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16780_length_3027_cov_5.524899"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PLG24_11640"
     CDS             <1..403
                     /locus_tag="PLG24_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HMZ25591.1"
                     /translation="KSTLDEARESDFLLHVVDLSHPGMEDQLITVQKTLHDLGAQDIP
                     TLIVGNKIDAYKKNLFDDFLSNAEKKEILAEQLEHFQRNFPQPWIFISAYTGENLDAL
                     KSLLADKVKEIYETKFPHQFQKEYNQNSFKQ"
     gene            414..2636
                     /locus_tag="PLG24_11645"
     CDS             414..2636
                     /locus_tag="PLG24_11645"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HMZ25592.1"
                     /translation="MLNVNQTWKKWAGMLFLALLSISVSTAQVKMTKKKSKETKAVAP
                     QNVPMSQINDYSGYYPYVEYGKFDNGKMWTFSYPPVQYFKETYGFTPSEEWLENMRMS
                     ALRFSTYCTASFVSEDGLVMTNHHCGRQSVTEVTKDGEDLPKDGFYAATLEEERKVPG
                     LFVDQLIAMKDVTSDILMAMSNGKDDDEKNKLKNEKINELVKENETATGIKVQVHTFY
                     NGSIYQMYTYKRYSDVRLVMAPETDLGYFGGDPDNFTYPRYSLDYTFFRVYDEDGKPL
                     KTKYHYTWSESGAKENEPLFVVGNPGTTQRLSTVSMLEYYRDYTMPHTLDLLNMLGST
                     YKEYIDSHPESKLELTDTYFGYMNSLKAYNGMHGGLKNPDLMGKRASFEKAFKTAVLE
                     KPELKAKYGDIWAKINASRNQMKQYFGKQIMFSPSGSTSKLFALGARAIDIATEMKKP
                     ENERTQGNSDEQLKAKIEKLKLSKSYYPQIEEKAIEFQLTKISKWSGNKMRMIDGTNN
                     PVMIPAQAKAIVANSVVANHEKLMALFNEGPDAVLNSNDPVLAYCKMSKDSLAYYKTI
                     IDPIAAEENKNIQLLGQAFYEVYGTSTPPDANFTLRIADGVLKTYDYNGTTAPEFTTF
                     YGLFDRYYSYGKKEPWNLPQRWLDAEKYMNKSTKYNYVTTHDIIGGNSGSAMVNSKGE
                     VVGLIFDGNIESLPGRYIFDMKSNNRCVAVHSDGIIETLRSVYKAGRIADELKNAKIK
                     "
     gene            2734..>3027
                     /locus_tag="PLG24_11650"
     CDS             2734..>3027
                     /locus_tag="PLG24_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ25593.1"
                     /translation="MKVCGFTIIRNAVKYDYPVVEAITSILPACDKFIVLIGNSDDDT
                     LDLIQSIHSEKIEIHHSVWDDSLREGGRVLALETNKALDLIPSEYDWAFYIQSD"
BASE COUNT         1003 a          608 c          621 g          795 t
ORIGIN      
        1 taaaagcaca ttggatgagg caagggaaag tgattttctg ctgcatgttg ttgatctttc
       61 tcatccgggc atggaagacc agctgattac cgtacaaaag accctccatg accttggcgc
      121 ccaagatatt cccaccctga ttgtgggcaa taagattgat gcgtataaaa aaaatctttt
      181 tgatgacttc ctttccaatg cggagaaaaa agaaatcctg gctgaacagc tggaacactt
      241 ccaaagaaat tttccgcaac catggatttt catttcggct tataccgggg aaaatttgga
      301 tgccctcaaa tcactgcttg cggataaggt taaggaaata tatgaaacca aatttcctca
      361 tcaattccaa aaggaatata accagaattc atttaaacaa taaattatag actatgctga
      421 atgtaaatca aacatggaaa aaatgggccg gaatgctttt cttggctttg ctatccatct
      481 ccgtctctac cgctcaggta aaaatgacga agaaaaaaag caaggagaca aaagcagttg
      541 ctccgcaaaa tgtaccaatg tcacaaatca atgattattc cggatattat ccttatgtgg
      601 aatacggtaa gtttgacaac ggtaagatgt ggaccttttc ttatcctcct gtacaatatt
      661 tcaaagaaac ctatggcttc accccttctg aagaatggct cgagaatatg aggatgtcag
      721 cgctcaggtt ttctacatat tgtactgctt ccttcgtctc tgaagatggg ctggtcatga
      781 ctaatcacca ttgtggtcgt caaagtgtca ctgaagtaac caaggatggc gaggatctcc
      841 ctaaagatgg tttctatgca gcaacattgg aagaagagag aaaggttccg ggtctttttg
      901 tagatcaatt aatcgctatg aaagatgtca cttctgatat ccttatggca atgtcaaacg
      961 gaaaagacga cgatgagaaa aataaactga agaatgaaaa aatcaacgaa cttgtaaagg
     1021 aaaatgaaac agctaccggc attaaagttc aggtccacac tttctacaat ggaagtatct
     1081 atcaaatgta cacttataaa agatattctg atgtgagact ggtcatggcc cccgaaaccg
     1141 atctgggata ctttggcggt gaccctgata attttactta cccacgttat agccttgact
     1201 acaccttctt cagagtatat gatgaagatg ggaaaccact gaaaaccaaa taccactata
     1261 cctggtccga atcaggagca aaagaaaatg aacctttgtt tgtagtaggc aaccctggta
     1321 ccacccagcg cctcagtacg gtttccatgc tggaatacta cagggattat acgatgccac
     1381 acaccctcga ccttctcaat atgctgggct ctacctataa agagtatatt gactctcacc
     1441 ctgaaagcaa actcgaactt acggatactt attttggcta tatgaatagt ttgaaggcat
     1501 ataacggaat gcatggcggt ctcaagaatc cagatttgat gggcaaacgt gcttcttttg
     1561 aaaaagcttt caaaacagca gttttagaaa aaccggagct taaggcaaaa tatggtgata
     1621 tctgggctaa aatcaatgca tcaagaaatc agatgaaaca atattttggt aagcagatta
     1681 tgttctcacc atcgggttca acctctaaat tatttgctct gggtgcaaga gcgattgata
     1741 tcgccacaga gatgaaaaag cctgagaatg agcgcacaca gggcaatagc gatgaacaat
     1801 taaaagctaa aattgaaaaa ttgaaactaa gcaagagtta ttatcctcaa atcgaagaaa
     1861 aagcaataga gtttcaattg accaaaatca gcaaatggtc cggcaataaa atgcgtatga
     1921 ttgatggaac caacaatccg gtaatgattc cggcgcaggc taaagctatt gtcgcaaatt
     1981 ccgttgtagc aaaccacgaa aaattaatgg cgcttttcaa tgaaggtccc gatgccgttc
     2041 taaattccaa cgatcccgta cttgcttatt gtaaaatgtc taaagacagc cttgcttact
     2101 ataagacaat cattgatcct attgcagctg aggaaaacaa gaatatccag ctattgggcc
     2161 aggcattcta tgaagtatat gggaccagca caccaccgga tgccaacttc acactcagaa
     2221 ttgctgatgg cgtgttgaaa acatatgatt ataatggcac aactgcgcca gaatttacta
     2281 ctttctacgg cctttttgac agatattatt cctatggtaa aaaggaacca tggaatctgc
     2341 cacaaagatg gctcgatgct gaaaagtata tgaacaagag caccaaatac aactatgtta
     2401 ccacccatga catcattgga ggtaacagtg gcagtgccat ggtcaacagc aagggagagg
     2461 tagtaggatt gattttcgac ggaaacatcg aaagtctccc tggcaggtac atcttcgata
     2521 tgaagagcaa caaccgttgt gtcgcagttc attctgatgg cattatagag actcttcgct
     2581 cagtctataa ggcaggcaga attgcagatg aattgaaaaa tgcaaaaatt aaataaggca
     2641 taaaaagcaa gaaaaagcaa gaatggcctg gctttcaaca aagttcaggc cattttattt
     2701 aatatattca atccttttta ctaaatttca tcaatgaaag tctgtggatt taccatcatc
     2761 agaaatgccg tcaaatacga ttatccggtc gtggaagcta tcacaagcat acttcccgct
     2821 tgcgataagt tcattgttct tattggcaat agcgacgatg atacattaga cctgatccaa
     2881 tccatccatt cggaaaagat tgagatccat cactcggttt gggatgattc attgagagaa
     2941 ggtggccggg tcctcgcatt ggaaaccaac aaggcattgg atctcattcc gtcggaatat
     3001 gattgggcat tttatatcca gagtgac
//
LOCUS       DAOQQN010000368         3011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_16922_length_3011_cov_5.320027, whole genome shotgun sequence.
ACCESSION   DAOQQN010000368 DAOQQN010000000
VERSION     DAOQQN010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16922_length_3011_cov_5.320027"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PLG24_11655"
     CDS             complement(<1..519)
                     /locus_tag="PLG24_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25594.1"
                     /translation="MINTSRTRVLDFVLRNFGSIASLCKWGMNAGVYLTISGLFASRF
                     LIACGMIVMGCASLLNMTTLHKQDIRIRLHLVKPVAIAVSLLVLGVVTSFLGSEQLGF
                     AMVRLRLYVAFLLIPLILAFAPPIDRNHIRNYLLYALSSAAISSIFIQLNYFQHYETI
                     SKELSTGKPIPTP"
     gene            complement(562..2181)
                     /locus_tag="PLG24_11660"
     CDS             complement(562..2181)
                     /locus_tag="PLG24_11660"
                     /inference="COORDINATES: protein motif:HMM:NF024683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 associated domain-containing protein"
                     /protein_id="HMZ25595.1"
                     /translation="MMVYYRKKLVILLFMATVVISSFGFKPPLKGRADLPNVLIIEIK
                     GMTFEMMERAYTPNIKFLIKNGFLGSMKYKNPEEPSTGISMNFRRKLGTMAMCMDSMK
                     VCQTSLNTVGAFNESFKGPFSKIRKNYRSNESMVKDFINNSNGTNVRFAMLSFRAFTQ
                     KQGFVDEGQIRELSTIDSLIGVVFKSYQEKVKWEQTTVFIVSDGSAAHEYRESELYKN
                     TPIVIKGPGIPIVYNVENVVSTDDVLGLVNKIMEVPCKDNFIPLPVNFLEDIGKDEEV
                     SGVKTYFVSRPDIHVHPIPSKNSLKIEVFDDNESSVMYYTIDGSDPRTRGFEYKSPIT
                     IREAGTYTIKAAANKNGVWSPVTQEKTSLRFNITSIELDPEPDAKYFYDGTDELIDGD
                     TAGLSYSEEKWLGFQGTDVDMLFNFGSKREIRSVDLSALHDYYSWIFLPVRVTFYAGN
                     DRNHLVEIGTISHAVDRNDVTIRKLHYSVIMHDALLKSLGGSIVKKKKGKRSLYVKYL
                     RIKVEAQKVAPEWFSLPGAQVWFFTDEVRIE"
     gene            complement(2184..>3011)
                     /locus_tag="PLG24_11665"
     CDS             complement(2184..>3011)
                     /locus_tag="PLG24_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HMZ25596.1"
                     /translation="QTDGVFVEQLIRPTGLVDPPIEVRPSINQIDDLMEEVAVRIERN
                     ERVLVTTLTKRMAEELAKYFQNLNIKTKYIHSEVDTLERVEILRDLRIGEFDVLVGVN
                     LLREGLDLPEVSLVAIIDADKEGFLRNTRSLTQTAGRAARNVNGKVIFYADKITDSMQ
                     KTIEETNRRRAKQLAYNEKHNITPKTLAKTKEEILSRSSILDIRGKKQEAYYQEAEHP
                     DIAADPVVAMMSQDQLMALITETENKMKKAAKDLDFLSAAQFRDELFALKKKLKFAS"
BASE COUNT          848 a          706 c          573 g          884 t
ORIGIN      
        1 aggtgtcggt attggtttac cagttgaaag ttccttgctg attgtttcat aatgctgaaa
       61 gtagttcaac tgaataaaaa tactggaaat agcagcactt gacagagcat acaacagata
      121 atttctgata tgattcctat cgataggcgg tgcaaaggcc aggataagtg gaatcaacag
      181 gaaagccaca tacaacctca accgcaccat tgcaaaacca agctgctcac tccctaaaaa
      241 ggaggtaacc actcccagca caagcagaga tacggcaatt gccaccggct ttaccaaatg
      301 taagcgaatc cggatgtctt gtttgtgtaa tgtagtcatg tttaacaagg atgcacatcc
      361 catcacaatc attccacagg caatcagaaa tctactggca aacaagccgg aaatggttag
      421 gtatacaccg gcattcatgc cccacttgca taatgacgca atacttccaa aattgcggag
      481 gacaaaatcc agtacccttg ttcttgaagt atttatcata ttttgccgga aactaaagct
      541 tatgcctgta agaaataaag tttattcaat cctgacttcg tccgtgaaga accaaacctg
      601 agcgcccggc aaagaaaacc attcaggtgc aactttctgg gcttccacct tgatccgtaa
      661 gtatttaacg tacaagcttc ttttgccttt cttcttcttc acgatactcc ctcccagtga
      721 tttcaggagt gcgtcatgca tgattaccga gtaatggagt ttcctgatag ttacatcgtt
      781 cctgtctaca gcatgagaaa tagtcccgat ctctaccaga tgatttctgt cgttacctgc
      841 ataaaatgta actctgacag gcaggaaaat ccaagaataa tagtcatgca aggctgacaa
      901 atccacagac ctgatctcac gcttcgaacc aaaattgaag agcatatcga catccgtacc
      961 ttgaaaaccc aaccattttt cctcggaata actgagacct gcagtatcgc catctatcaa
     1021 ctcatcggtc ccatcataaa aatattttgc atcaggttcc gggtctagtt ctatcgaggt
     1081 aatattgaac ctcaatgaag tcttttcctg cgttacaggt gaccacacac catttttgtt
     1141 tgctgcggcc ttgatggtat aggtaccagc ctctctaatg gtaatcggtg atttgtattc
     1201 aaatcctctg gtgcgtggat cgcttccatc gattgtataa tacatcaccg atgactcatt
     1261 gtcgtcaaaa acctcgattt tgagagaatt ttttgaggga ataggatgca catgaatatc
     1321 aggccggctt acaaagtagg tcttcacccc ggagacctct tcatctttgc caatatcttc
     1381 caggaaattg accggaagtg gaatgaaatt atctttacaa ggtacctcca taatcttgtt
     1441 gactagacca agaacatcat cggtagatac cacattctcc acattataca caattggaat
     1501 tccgggtccc ttgattacga ttggagtgtt tttatataac tcactttcac gatactcatg
     1561 ggctgcagat ccgtcagaga caatgaagac agtggtttgt tcccatttga ctttctcttg
     1621 gtagctctta aaaactacgc ctataaggct atcaatggtt gaaagttccc tgatctgccc
     1681 ctcgtccaca aatccttgtt tttgcgtaaa tgccctgaag gaaagcatgg caaacctgac
     1741 attagtacca ttgctgttat tgataaaatc cttcaccatc gattcattgg accggtaatt
     1801 cttcctgatt ttactgaaag gacctttgaa agattcattg aaagcaccga ctgtgttgag
     1861 agaagtttga caaaccttca tggaatccat acacattgcc atagtgccta acttacgcct
     1921 gaaattcatg gaaataccgg tagaaggttc ctccggattc ttgtacttca tcgaaccaag
     1981 gaaaccattc ttgatgagga atttgatatt tggagtataa gcacgctcca tcatttcaaa
     2041 ggtcatgccc ttaatctcaa taatcaacac attcggcagg tcggcccgtc ctttcaatgg
     2101 tggtttaaat ccaaaagagg agataaccac agttgccatg aagagcaata tcacaagctt
     2161 ttttctataa taaaccatca tttctagctt gcgaatttta atttcttttt caaggcaaat
     2221 aattcatccc ggaactgtgc agcggaaagg aaatcaaggt ctttggcagc ttttttcatt
     2281 ttattttccg tctcagtaat caatgccatc aattgatcct gtgacatcat agctacaacg
     2341 ggatctgcag caatatcagg gtgttcagcc tcctggtaat atgcttcctg ttttttgccg
     2401 cggatatcca gaatcgaact tcgggatagg atttcctctt tggtcttggc caaagtctta
     2461 ggggttatat tgtgtttctc attatatgcc aactgttttg cccgtcgccg gttggtttct
     2521 tcaattgttt tctgcatgct atccgttatc ttgtcagcat agaaaataac cttaccattt
     2581 acatttctgg ccgcccttcc ggctgtctgt gtcagagacc gcgtatttct caggaagcct
     2641 tccttatcag catcaatgat cgcaaccaat gacacctcag gcagatccaa accctctcgc
     2701 agcaagttta ccccaaccag tacgtcaaat tctccgatac gaaggtctcg taatatttct
     2761 actctttcga gggtatccac ttctgagtgg atgtacttgg tcttgatatt aagattttga
     2821 aagtatttag ctaattcctc agccattcgt ttggtgagcg ttgtaaccaa aaccctctca
     2881 ttgcgttcta ttctcacagc cacttcttcc atcagatcat caatctgatt gatggaaggg
     2941 cgaacttcaa tcggaggatc aaccaagccc gtaggcctga tcaactgttc cacaaagaca
     3001 ccgtcagtct g
//
LOCUS       DAOQQN010000369         2964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_17360_length_2964_cov_6.059127, whole genome shotgun sequence.
ACCESSION   DAOQQN010000369 DAOQQN010000000
VERSION     DAOQQN010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17360_length_2964_cov_6.059127"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /gene="ftcD"
                     /locus_tag="PLG24_11670"
     CDS             complement(<1..743)
                     /gene="ftcD"
                     /locus_tag="PLG24_11670"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HMZ25597.1"
                     /translation="MSQILIECVPNFSEGRDQSVLDAISEAVRSVDGVSLLDVDPGKA
                     TNRTVFTFVGHPDAVVEAAFEAIAVAGRLIDMSKHNGAHPRMGATDVCPLIPISGISI
                     EELVPYAHKLGKRVAEELGIPVYLYEAAATSPQRKSLGDIRSGEYEGLAVKIKDPVWA
                     PDYGKAEFNAYSGATVIGVRDFLVAYNINLNSSSVKKANAIAFDVREQGRILKKQGET
                     VIDKNGEPVRQPGKLKSVKGIGWYIEEYGV"
     gene            complement(2106..>2964)
                     /locus_tag="PLG24_11675"
     CDS             complement(2106..>2964)
                     /locus_tag="PLG24_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMZ25598.1"
                     /translation="LPDYIQVWPAHGAGSACGKALGAVHSTTVGYEKIRNWAFRFGED
                     ETGFVRALLEDQPEPPRYFAMMKHLNKIKRPLLVEVPKYPKLKKEQFLTAYNHGLKII
                     DTRSKEDFARGFLPGSIGIQGNNSFSTWAGWFLDHQEQFVLIADEERIDDLTRKLMRI
                     GLDNVYGYYSDIEDLGLELQKVRSVSYEMMLDIIDGEDFQVIDVRGYTEFEAGHIEGA
                     ENLFVGTIEQHLDKIDRSRKVVVYCQGGDRSAIAQSILSRHGFTSIFNYPGGMNEWNE
                     KTAKVPVYL"
BASE COUNT          877 a          646 c          571 g          870 t
ORIGIN      
        1 acgccatatt cttcaatata ccaaccaatg ccctttacac tcttcagttt gcctggttga
       61 cgcaccggtt caccgttctt atcgattacg gtttcacctt gtttcttgag gatacgaccc
      121 tgttcacgca catcgaatgc aatagcattg gccttcttga ctgatgaaga gttaaggttg
      181 atgttgtagg cgaccagaaa atcccttacc ccgatcacag tcgccccgga ataagcattg
      241 aattcagcct tcccataatc cggtgcccac acgggatctt taatttttac agccaagcct
      301 tcatattcac cacttcggat atcacccagg ctcttacgct gtggtgatgt tgcagctgct
      361 tcataaagat acaccggaat tccgagttct tctgcaacgc gttttcccaa cttatgtgca
      421 taaggcacca attcttcaat agaaataccg gaaattggaa tcagcgggca aacatctgtg
      481 gctcccatac gaggatgcgc cccgttgtgc ttgctcatgt ctatcaaccg tcccgctacg
      541 gcgatcgctt caaatgctgc ttcaacgact gcatccggat gacctacaaa ggtaaaaacc
      601 gttctattgg ttgccttccc cggatcgaca tccagcaagg atacaccgtc aacactacgt
      661 accgcctctg agatggcgtc taaaactgat tggtcgcgac cctcactgaa gttagggacg
      721 cattctatca atatctggct catgattaaa ttatttattc agaagctaca aatataggat
      781 gtttttaggt tggctgagaa aagaaaaagc aggtatggtc atgaactttt aaaattgatt
      841 atgaaaaaac atcctgtaaa taattctcct atgtatattt tatgaaactg ctaataatct
      901 acagtaatga aatattgcaa tgcttcgtca aggtagaata gtcaattaaa tataacacaa
      961 tgtaattcca actttcaata taaagatacc aacagaggaa atcaagaaag ccaaatgttt
     1021 tatgttgaaa gaatgtaaaa aatgcccggt ttgaaacaag ggttaaaaca tttgaagtgt
     1081 tgagatcgaa agtgatattg agcgtagaaa tagttcgcaa aaaaatcagt cccctaaaaa
     1141 cactggcaaa taatcagttt acggtttcct ttcggtgttg aatcctatga ttccggcggt
     1201 aaaaaattat ttcaaaaatc gtgaaacata ttaagaatta tttcaaaaaa gtaaaaaggg
     1261 aatagtccta gatattctca ttgtaatcca aagtaagatc tttgtcgcca tctatttttt
     1321 gggattagta tgctaagagg attttggtcg tattgctccg aataaccaga agattatttt
     1381 gatattgcat tacaaaattt catttgcttt ttaaccttaa tcaacaggcc gcttaaacgg
     1441 ttcttctatc attttgatcc gaagctttag taaatatcat acaggcccgt tggcttttat
     1501 taatatggtg ctttgattta atcattatca acattactgt aatgggatag caacctattt
     1561 tccgggcata gcataaacag tgcactgatt aatagtcagc cgcctcaaaa aatgtgaaat
     1621 tctgagcgat caagcgggac tcaaagtgta ggcaatcaaa tctgacctaa ggccctgttg
     1681 gtcaagacag gttaatatgc aatcatggat cgacaacaag aatgaatagg gtccatttca
     1741 tccggaaata ggcaccaaat atttacgaac ttgatacaaa ttgattatat tcatggaaca
     1801 atggagcatt cataaattat accaagatga tttttataat agtccataaa tttttggtat
     1861 acttgtccgg atagatatgc ttccagagct ttgctctgta ccgggagtga aacaatccgg
     1921 ggttccggga taacgaatgt accttcagtt ttactcttat ttcatgaaat tcttccgcaa
     1981 aaaaaagagc cccgcgatat tgatagcggg actccatcat ttaaggcttt ctgattggaa
     2041 aaagatgtcg tttttcaaaa agccacattg acttaaaaaa aagcttgatt gttaattatt
     2101 ttattttata agtataccgg gaccttggcg gttttttcat tccattcatt catacctccc
     2161 ggataattaa atatactggt gaagccatgt cttgatagaa tggattgcgc gatggccgaa
     2221 cgatctcccc cctgacaata cacaacaacc tttcggctgc ggtcaatctt gtccagatgt
     2281 tgttcaatag tgcccacgaa cagattttca gcaccttcaa tatgtccggc ttcaaattca
     2341 gtataaccac ggacatcgat aacctgaaag tcctcgccgt caatgatatc caacatcatc
     2401 tcatacgata ctgaccttac tttctgcaat tccaggccga gatcttcaat gtcggaatag
     2461 tagccatata cgttgtccag gccaatgcgc atcagtttgc gcgtgaggtc gtctatgcgc
     2521 tcttcatccg caatcaaaac aaattgctct tgatgatcga ggaaccaacc tgcccaggtc
     2581 gaaaacgaat tattgccctg gatacctata cttcccggca ggaagcctcg cgcaaagtcc
     2641 tctttgctgc gggtatcgat gatcttaagg ccgtgattgt atgcggtcag aaactgttct
     2701 ttttttagtt taggatattt cggcacttct acaagcaatg gcctttttat tttgttgagg
     2761 tgtttcatca tagcgaagta tcgaggtggt tcaggctgat cctccagcaa ggccctgaca
     2821 aaaccggtct cgtcttctcc aaagcggaat gcccagttgc gtattttttc ataacccact
     2881 gttgtactat ggactgcgcc caaagccttg ccacaagccg aacctgcacc atgagccggc
     2941 catacctgga tgtaatcagg caat
//
LOCUS       DAOQQN010000370         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_17490_length_2950_cov_4.574439, whole genome shotgun sequence.
ACCESSION   DAOQQN010000370 DAOQQN010000000
VERSION     DAOQQN010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17490_length_2950_cov_4.574439"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLG24_11680"
     CDS             <1..349
                     /locus_tag="PLG24_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25599.1"
                     /translation="TNKGRALATGNTIYIYDGLSITKDQPTDRPVRHSLKVSPNPAKD
                     RIRLDIFLPNPTSLRLDLLDEQGYLIRQLFRGGVPAGNISKEYSIGNLSPGTYVIWMR
                     TNEGHLSGKLIVY"
     gene            440..1006
                     /gene="frr"
                     /locus_tag="PLG24_11685"
     CDS             440..1006
                     /gene="frr"
                     /locus_tag="PLG24_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HMZ25600.1"
                     /translation="MNLEEANSILDKAKKMMDNAIEHLNHELSKVRTGKASPSLVAEI
                     KVEYYGSATPLSQVANVNISDAKTIVIQPWEKSMLRTIENALFEANLGITPQNDGEVI
                     RLTIPPLTEERRRDLVKQAKHLAEEAKVSIRNVRRDGMESIKKLVKDGFPEDLGKKKE
                     DELQKLTDHHTEQSGKLAEAKEKDIMAI"
     gene            1044..2495
                     /locus_tag="PLG24_11690"
     CDS             1044..2495
                     /locus_tag="PLG24_11690"
                     /inference="COORDINATES: protein motif:HMM:NF039502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HMZ25601.1"
                     /translation="MSDKLNTSLLKGISEQPLENLAGLDDAAFDTISDIDKFLINDTL
                     LTYAIKGEEFPYELTAAGEEKFPVGPRCLIDLYHQMGYNSFASAGSLIIRRHLQLKCL
                     LQPPLIIPILEHAHDWSDYIFFLVKIIRERGRWLCEARTEWHWWKDALNPGIMEQKDN
                     VNRYLYFRLLRFFRYEEANSLLVNLWTNERKSTKQLFVDLLRENLSGGDVETLRYLWE
                     NESGKIRHEIRKLIFFASKNGEFFRLKKRLFSWEIKGTKAGEWMADLPIVAKFYDIDE
                     PFPAEWSAYVNFADKRKKKSGNDAFAAMPEKASERQRAFGDVLYATLVDGLFESLGRW
                     VSRIWSDQPAYRLGSDWIGYFNHLEEKKKNIVLQHIIHHGDDEATVRFSAQLMSRCLI
                     EMDKDSTSALLRLIKKVLQQPVCRLDDRWATEVKAYAPFVIHESYLGDWVDIGNLITR
                     KIVSAKTVNPSAETLKLIIFRRKIDTCFHESAK"
     gene            2479..>2950
                     /locus_tag="PLG24_11695"
     CDS             2479..>2950
                     /locus_tag="PLG24_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ25602.1"
                     /translation="MNQQNEIRPSAEVRYGEELKQLASHDKYEKPEGWKLSPKAVIHY
                     LVGGRLDNDVVIEPKYLGNRKLLELCIATLLTDRGLLLMGLPGTAKSWLSEHLAAAIS
                     GISTSMIQGTVGMDENQLKYHWNYAELLQHGPSERALVKSPVLRAMEDGYITRIE"
BASE COUNT          864 a          601 c          717 g          768 t
ORIGIN      
        1 aactaataaa gggagagctc ttgctacagg caatacgata tatatctacg atggcctcag
       61 catcacaaag gaccagccga cagatcggcc tgtcaggcat tcactcaagg tatcccccaa
      121 tccggctaag gatcgtatcc ggctcgacat ctttctcccc aatcctactt ctctgagact
      181 tgatctgttg gatgagcagg gttatctcat acgtcagttg ttccgtggcg gggtgccggc
      241 gggcaatatt tccaaggaat attccatagg gaatttatct ccgggaacat acgttatctg
      301 gatgcgtacc aatgaaggcc atttgtccgg taagcttatt gtctactgat ggcaaggggc
      361 aagatatgaa tcattttaag cggtaatttt cttttttcag ttttaaaata tatttttacg
      421 tccgaaaaat gtttaaatta tgaatttgga agaggcaaat agcattcttg acaaggcaaa
      481 aaaaatgatg gataatgcca tcgaacacct taatcatgaa ttgtcaaagg taaggaccgg
      541 gaaggcttcc ccatctttag ttgctgagat taaggtagaa tattacggca gtgcaactcc
      601 tttgagccag gttgccaatg tcaatatttc agacgccaag actattgtta tccagccctg
      661 ggagaaatct atgttgcgta caattgaaaa tgccttgttt gaagctaatc tgggcatcac
      721 accgcaaaat gacggtgaag tcatccgcct gactattcca ccccttacag aagaacgccg
      781 gcgtgacctg gtgaagcaag ccaaacatct tgcggaggaa gcaaaagtaa gtatccgcaa
      841 tgtgcgaaga gatgggatgg aaagtatcaa gaagctggtc aaggatggtt ttccggagga
      901 tctgggcaag aagaaggaag atgaattgca gaagttgaca gatcaccata ccgaacaatc
      961 cggcaagctg gcagaagcaa aagaaaagga tatcatggcg atttaatgcc gatttccttc
     1021 agtcaccttc cattagaatt gtcatgtccg acaagttaaa tacatccttg ctgaaaggta
     1081 tttctgaaca gccgctggag aatcttgcag gcctggacga tgctgccttc gacacaatat
     1141 ccgatataga taagtttctg atcaatgata ctttgctaac ctatgccatt aaaggggaag
     1201 aattcccgta tgaattgaca gctgccggag aggaaaagtt tccggtcggt ccacgctgcc
     1261 tgattgatct gtatcatcaa atgggatata atagctttgc aagtgccgga tccctcatca
     1321 tccgccggca tctgcaattg aaatgcctgc ttcaaccgcc cttgatcatt ccgattctgg
     1381 aacatgccca tgactggtcc gattacatct tttttttggt aaaaatcata agagaacgtg
     1441 gtcgatggtt atgtgaggcc aggactgaat ggcattggtg gaaagatgct ctcaatccgg
     1501 gcatcatgga acaaaaggac aatgtcaacc gatatctgta tttccgattg cttcgatttt
     1561 tcaggtacga agaggcaaac agccttctgg taaatttgtg gaccaacgag cgtaaatcga
     1621 caaaacagct ttttgttgat ttattgaggg agaatttgtc aggaggagat gtagagactc
     1681 tcaggtactt gtgggaaaac gaaagtggaa aaatccgcca tgagatccgt aaattgatat
     1741 tttttgcctc caaaaatggg gaatttttcc ggcttaaaaa aaggctcttt tcatgggaga
     1801 taaaagggac aaaagccggt gaatggatgg cagatttgcc gatagtagct aaattttatg
     1861 atatcgatga accatttcct gctgaatggt cggcctatgt aaactttgca gacaaaagaa
     1921 agaagaaatc cggaaatgat gcatttgccg ctatgcctga aaaagcatct gagcgtcagc
     1981 gagccttcgg tgatgtgttg tatgccacgt tggttgatgg tttgtttgaa agccttggac
     2041 gatgggttag caggatttgg tcagatcagc ctgcttatcg gttgggatca gactggatag
     2101 gttatttcaa tcatctggaa gagaaaaaaa agaatattgt gctccaacac atcatccacc
     2161 atggtgatga tgaggctaca gtacgttttt ctgcgcaatt gatgtctcgt tgtctgattg
     2221 aaatggataa ggatagcaca tccgccttgt tacgacttat aaagaaggtt ctgcaacagc
     2281 ccgtatgtcg tctggatgat aggtgggcca cggaagtgaa agcctatgcc ccatttgtaa
     2341 tccatgagag ttatctgggc gactgggtag atataggcaa cttgattaca aggaagattg
     2401 taagtgccaa aacggtgaat ccatcagcag aaacattaaa attgattatt tttaggagaa
     2461 aaatagatac ctgcttccat gaatcagcaa aatgaaatca ggccgagtgc cgaagttcgc
     2521 tatggcgaag aactaaagca actcgcttcc catgataaat acgagaaacc cgaaggctgg
     2581 aagctttcgc ctaaggctgt catccactac ctcgtcggag gccgactgga taatgatgta
     2641 gtcatcgagc ctaaatatct cggcaataga aaattactgg aattgtgcat agctacatta
     2701 ctgaccgaca ggggcctgct cctcatgggt ttgcccggca ccgccaagtc ctggctttcc
     2761 gagcacctgg cagctgctat ttcggggatt agtacttcga tgatacaagg tacggtgggt
     2821 atggacgaga atcaactgaa atatcattgg aattatgccg aactacttca gcatggaccc
     2881 tcggagcgtg cattggtcaa aagccctgtc ctgagagcca tggaagacgg ctacatcacc
     2941 cggattgaag
//
LOCUS       DAOQQN010000371         2932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_17665_length_2932_cov_5.519639, whole genome shotgun sequence.
ACCESSION   DAOQQN010000371 DAOQQN010000000
VERSION     DAOQQN010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17665_length_2932_cov_5.519639"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            451..693
                     /locus_tag="PLG24_11700"
     CDS             451..693
                     /locus_tag="PLG24_11700"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HMZ25603.1"
                     /translation="MSKDAGKTILALFAGLAAGVALGVLLAPEKGEDTRHKISDATNK
                     ITNDLRDKVLEGINKLRAKKDQYAQATSDLADEILS"
     gene            820..1194
                     /locus_tag="PLG24_11705"
     CDS             820..1194
                     /locus_tag="PLG24_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25604.1"
                     /translation="MTNVNVKSVDNNKKSYDTDFDLMELAFDVLVPFILDNFVFKGTK
                     GRLVKLVGTVAAQQLLKMLAKSQVVDDILDYIEELLLPEERKPAKPFPSLVDAVSQTA
                     PKKYWNKSKPEDYYDPEREMFS"
     gene            1343..2278
                     /locus_tag="PLG24_11710"
     CDS             1343..2278
                     /locus_tag="PLG24_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25605.1"
                     /translation="MEVTRSQAISMARTEFLLLKDNLSRKSWKESHLYILETNGMMVE
                     FPLNPGVNKLNNSDFGNFMSALDVQDVLVVSMCDHEGVTYVASFSILADQPDYHPPFY
                     VSPYSRIHRFPYQLVTSSEGKSQILKLDTTLAENKKVMDAYLKMPRVSIQHYPKFETV
                     SNYLSANDTIIQLGKRNFEIHPINKPVDDLLLITTEPKEIVDAADMYLDWNKMIVKPD
                     GPAFSLAFLKNIQSHSISFFANDKKYEIQSMRFTYLDGKKLNKGYYWKNTMTYPIEPI
                     RDLNNPFSILIDRVIIKDARLGYVYIPQIFLFSFQ"
     gene            2357..>2932
                     /locus_tag="PLG24_11715"
     CDS             2357..>2932
                     /locus_tag="PLG24_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HMZ25606.1"
                     /translation="MKRHKYVFKFGGASIRDVDGIRNVAKILEQYAADDLAVVISASG
                     KTTNHLEEVMEAYFNNTGELDSKLNLVKNHHKALMDGLFQPNDPVYKDVEDIYHEIDW
                     IYDDRNSTDYDFLYDQLVSLGELLSTRIVAAYLNSIGVQTHWADARKLVITDDTYRES
                     QINWEETMAQIRNSIQPLLSQGKIVLTQGFIG"
BASE COUNT          932 a          489 c          573 g          938 t
ORIGIN      
        1 taatatccgg ggatgaaaag ttgtatccct cttgaatatg ttttataaaa ctttcctggt
       61 taagtgacat ggtctggaat ttgaaaacaa atatgaggta aagttagaaa tatttatcct
      121 ccaaagattc aataaatttt atatacattt aaagcataat attttaacaa tgaaaataca
      181 aggctaattc aaggtggtct cacatatcat attttgtaac agattgacat acattggtat
      241 gtgtccactt taatttgaat attttggaaa aatagcaatt tctttggcat tatcaaattg
      301 ttagctaaaa attagccgta atgggaactt ttagtgtaaa atatttatta acttgatagt
      361 tggtttaatc gaatattcga aacaatattt tgttgtgaag ggcgtttaaa ccatgtgttt
      421 ttattttaat aacaacaaac ttatattatc atgagcaaag atgcaggaaa aacaatttta
      481 gcactgtttg ccggattggc agcaggtgtc gctttaggtg tacttttagc acctgaaaaa
      541 ggtgaggata cacgccataa gatatccgat gcaacgaata aaattacaaa cgatctacgt
      601 gataaggttc tcgagggtat caataaactg agagctaaaa aggaccaata tgcgcaggcc
      661 acttctgatt tggccgacga aatattgtct tgatatttgc aagattaatt atgtatgaag
      721 aataccgaaa gtatgcaaca ctttcggtat tttatttgtt acagccctat atgtgaatca
      781 ataacacatt atcttatatc ccatgttcaa aatattttta tgactaatgt gaatgtaaaa
      841 tctgtagata acaataaaaa atcgtatgat accgattttg atctaatgga gttggcattt
      901 gatgttttag ttccgttcat actggacaat ttcgtattca aaggaactaa aggccgtttg
      961 gttaaacttg ttggcactgt tgcagctcaa caattattaa agatgctggc taaatcacaa
     1021 gtagtggatg atattctgga ttatattgag gagttgttgt tgccggaaga acgaaaaccg
     1081 gccaagcctt ttccgagctt agttgatgct gttagtcaga cagctcccaa aaaatactgg
     1141 aataaatcaa agccggaaga ctattatgat cctgaacggg aaatgttttc ataaaaacga
     1201 agcatgaccg atgtataaat tttgccttgt ccctttattt ttaatatttt cttttcatgc
     1261 cttttccgaa aatattattc ttaattgggg agtttttaat gtacgcctga atccggtctc
     1321 ggtaaacact tatgttggtt atatggaggt tacacgatct caggcaatca gtatggccag
     1381 aacagagttt ttattactga aagataactt atcccggaag tcttggaaag aaagtcacct
     1441 gtatatcctg gaaacgaatg gaatgatggt ggagtttccg ctgaatccgg gtgtcaataa
     1501 actcaataat agtgattttg gtaattttat gtcagcattg gatgtacaag atgttcttgt
     1561 agtatcaatg tgtgatcatg aaggggtcac ttatgtggca agtttttcaa tccttgcaga
     1621 ccagcccgat tatcatccac ctttttatgt gtctccatac agcaggatcc atcgttttcc
     1681 ttatcaatta gtcacaagtt ctgaagggaa aagtcaaatt ttgaaactgg atactacttt
     1741 ggctgaaaac aaaaaagtaa tggacgccta tcttaaaatg cccagggttt caatccagca
     1801 ttacccaaag ttcgagaccg tctccaatta tctttcggca aatgatacta ttatccagct
     1861 cggaaagcgt aacttcgaaa ttcatccgat taacaagccc gtggatgatt tacttttgat
     1921 aacaacagag cccaaagaga ttgttgatgc agcagatatg tatctggatt ggaataaaat
     1981 gattgtgaag cccgatggtc cggcattctc tctggcattc ctgaagaata ttcaaagtca
     2041 tagcattagt ttttttgcaa atgataaaaa atacgagatt cagtctatgc gattcaccta
     2101 cctcgacggg aagaaattga ataaaggata ttattggaaa aataccatga cttatcctat
     2161 tgagccaatc cgtgatttaa ataatccctt ctcaatcctc atagacaggg tcatcattaa
     2221 ggatgccagg ttgggctatg tctatatccc gcaaattttc ttatttagtt ttcaataaat
     2281 tttcatcttc ttttgaaaat tcatttcttt tgtgcattgt ttcaaaacat tgaaatttaa
     2341 atctctggtt gattagatga aaagacacaa atacgttttt aagttcggtg gggcatctat
     2401 ccgggatgta gatggcatcc gcaatgttgc aaaaattctt gagcaatatg cagccgatga
     2461 tctcgctgtt gtgatttccg cttccggtaa gacaaccaat catcttgaag aggtgatgga
     2521 agcttatttt aataatacgg gagagcttga ttcgaaactg aaccttgtga aaaatcacca
     2581 caaggcatta atggacggct tgtttcagcc aaatgatccg gtctataaag atgtagagga
     2641 tatctatcat gagattgact ggatctatga tgatcgaaat tcaaccgatt atgacttcct
     2701 gtatgatcag ttggtcagcc tcggtgaatt gctttcaaca cgtattgttg ctgcctatct
     2761 gaatagcatt ggagtccaaa ctcactgggc tgatgctcgt aagctagtca taactgatga
     2821 tacatatagg gaatcgcaaa taaattggga agaaacgatg gcgcaaatca gaaattcgat
     2881 tcaaccattg ctttctcagg gcaagattgt attgacacaa ggttttatcg gg
//
LOCUS       DAOQQN010000372         2927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_17726_length_2927_cov_5.997214, whole genome shotgun sequence.
ACCESSION   DAOQQN010000372 DAOQQN010000000
VERSION     DAOQQN010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17726_length_2927_cov_5.997214"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..376
                     /locus_tag="PLG24_11720"
     CDS             56..376
                     /locus_tag="PLG24_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25607.1"
                     /translation="MDISANLARIIHRLQHESTLDEDWERFSHYFNEVNQGFILRLKE
                     KYPNLTPNDHRLAAYLRMNLSTKDIAALSNISIRGVEGSRYRLRKKMNLENDDNLHEI
                     MNSI"
     gene            complement(463..807)
                     /locus_tag="PLG24_11725"
     CDS             complement(463..807)
                     /locus_tag="PLG24_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HMZ25608.1"
                     /translation="MPYDNPSLTWDDFMKPDIRTGTILFAEAFPEARKPAYKITIDFG
                     EIGIKRTSAQVTGLYATDDLIGKQVVAVVNFPPKQIANFISECLLLGAVDGNYVTLLT
                     LDLPVRNGLRIG"
     gene            1060..1971
                     /locus_tag="PLG24_11730"
     CDS             1060..1971
                     /locus_tag="PLG24_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25609.1"
                     /translation="MRWKTLILIVPASRMLFVFCSLFMPSLCFGLHSDIKPSIGEPFS
                     TMRDTVYPGTDLAILKMDPLKKEGFPGEGIAINMIIMNYGFSESASTKIDFWVSEEDE
                     PDLDTNNINSTKLLAPLKMDTKVDFSHLVVIPADYTGRYVYITAVIRPNPKNGSDPTP
                     GNNTMFTAVRIGSADSYKGGIYSYDGAMKEITMVNPIRKLKRGKPYPFQVKFSITGVR
                     APKAGQYIDILLSADPVLDDTDATIASFPAIPGVNGVVKDIKKDLVFGKQFRKGKAYI
                     FAVIRPGKWKDPNMDNNYSKPLKIRLK"
     gene            1971..2741
                     /gene="surE"
                     /locus_tag="PLG24_11735"
     CDS             1971..2741
                     /gene="surE"
                     /locus_tag="PLG24_11735"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HMZ25610.1"
                     /translation="MKKPLILVTNDDGMVAPGIAALVEVARDFGQVFVVAPDSPQSGQ
                     GHAITVENPLRLNQVDVFEDIVAYECNGTPADCVKLAKHLLHKNQHIDLCLSGINHGS
                     NASVNIIYSGTMSAAMEAAIEGIDSIGFSLLDYSFDADFTTAKQVVRTILEKVTRHGF
                     DADCRLLNVNIPKVAPDELKGIKVCRQADGYWMEEFVVGTDPRNQEYYWLTGKFTNRD
                     KGEDTDLYALQNGYASIVPSHYDLTHYKSIEKYKVLEE"
     gene            2743..>2927
                     /locus_tag="PLG24_11740"
     CDS             2743..>2927
                     /locus_tag="PLG24_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25611.1"
                     /translation="MKANQPFFTKDSLILGVVIGTVVPIVLYALILMLKDALVSAGIV
                     PELFGEFRSSQRTIGIIA"
BASE COUNT          814 a          666 c          639 g          808 t
ORIGIN      
        1 ggttgatttt gaataatatt ctgtttccgg cggccaaaaa gaaaaaacaa aaaatgtgga
       61 catttctgcc aatctggcga ggatcatcca cagactccag cacgaaagca ctctggatga
      121 agattgggag cgtttttcac attatttcaa tgaggtcaat caaggtttca ttctcaggct
      181 aaaagaaaaa tatcccaatc tgacacccaa tgatcacaga ttagcggcat accttcgtat
      241 gaacctttcc actaaggata ttgccgctct ctccaacata tcgatccgtg gtgtggaagg
      301 aagtcggtat cgccttagga agaagatgaa tctggagaat gacgacaacc tgcatgaaat
      361 aatgaattca atatagaaca acgagctatt ttctccacag atatggttca acaagatgtt
      421 ttatttaggg ttgtaatatc cctttgccgc taaattcttt tgtcatccta ttctcaagcc
      481 atttctcacg ggcagatcaa gagtaagcag tgtaacataa ttgccgtcta ctgcccccag
      541 taacagacat tcactgatga aattggcaat ttgtttgggc ggaaaattga ctaccgcgac
      601 gacttgcttt ccgatcaggt catcggtggc atacaagccg gtcacttgtg ccgaagtcct
      661 cttgatgcct atttccccga agtctatggt aatcttatat gccggttttc tcgcctccgg
      721 aaacgcttct gcaaataaaa tggttccggt gcggatatcc ggcttcatga aatcatccca
      781 tgtgagggaa ggattgtcat agggcatggt taggttgttt aatggacaat attcttccaa
      841 aaataataca attcttccgc tcgaatttga tgcattatta aaacaaatcc ttattcaact
      901 tgtcatttat tgagaataaa tccttgtatt ttattaatat tacaaattga ttcgggaata
      961 ttgatgacat tcgatttatt taaccatttc attatttatc tgtggaaagg cattcctcct
     1021 caatatagag gcgccgcggc acccacattt gttgggttta tgcgttggaa aacgctcatt
     1081 ctgattgttc cggcgagcag gatgctattt gtcttttgtt ctctgtttat gccttccctg
     1141 tgctttggcc tgcattccga tatcaaaccc tccatcggcg agcctttttc taccatgagg
     1201 gacacggtat atccgggtac tgaccttgct atcctgaaaa tggacccttt aaagaaagag
     1261 ggtttcccgg gtgaaggcat agccatcaac atgattatca tgaattatgg cttctcagaa
     1321 tcggcatcta caaagattga tttctgggtt agcgaagaag atgaaccgga tctggatacc
     1381 aacaatatca attcgactaa attgcttgct ccgctcaaga tggataccaa ggtcgatttt
     1441 tctcatctgg ttgtcatccc ggctgattat acggggagat atgtatatat taccgcggta
     1501 atcaggccca atcccaagaa tggttccgat cccactcccg gcaacaacac gatgtttacg
     1561 gctgtcagga ttggaagtgc cgatagttac aagggcggca tctacagcta tgacggtgcc
     1621 atgaaggaaa tcaccatggt taatcccatc aggaaattaa aacgtggtaa accctatcct
     1681 tttcaggtca aattttctat aacgggtgtg agggcaccaa aagccggtca gtatatagac
     1741 atcctccttt cagccgatcc ggtcctggat gatacggatg ccacaatagc atcattccct
     1801 gcaattcccg gtgtaaacgg agtagtaaag gacattaaga aggatttagt cttcggcaaa
     1861 cagttcagaa aagggaaagc ttatatcttt gcagtcattc gtccggggaa atggaaagac
     1921 cccaatatgg acaataatta ttccaaaccg ctaaaaatca ggttaaaata atgaaaaaac
     1981 cattgatatt agttaccaat gacgatggca tggtcgctcc cggtatcgct gccctcgtcg
     2041 aggtggctcg tgatttcggc caggtatttg tcgtggctcc cgacagtcca caatcaggcc
     2101 agggacacgc catcactgtc gaaaaccccc taaggctcaa tcaggtggat gtgtttgaag
     2161 atattgtcgc ttatgaatgt aatggcacac cggccgactg tgtgaagctt gccaaacatc
     2221 ttcttcacaa aaatcagcac atcgaccttt gcctttccgg catcaaccac ggctccaacg
     2281 catccgtcaa tattatttac tccggaacta tgagtgcagc catggaggcg gctatcgaag
     2341 gaatcgacag cattggattt tcactgttgg attacagttt cgatgcagac ttcaccacag
     2401 ccaagcaagt cgtgcgaacc attcttgaaa aggttaccag acatggcttc gatgcagact
     2461 gccgcctgct taatgtcaat attcccaagg tggctcccga tgaacttaaa gggataaagg
     2521 tatgtcgtca ggccgatggc tattggatgg aagaatttgt cgtcggtacc gatccgcgca
     2581 accaggaata ttattggctg acgggaaagt ttaccaaccg tgacaaaggt gaagatactg
     2641 acctttacgc cctgcaaaac ggatatgctt ccattgtgcc atcccattac gacctgactc
     2701 attataagtc tatcgaaaaa tacaaagtcc tggaagaata atatgaaagc caaccaaccc
     2761 ttttttacta aagactcttt gattctgggc gttgtcatag ggaccgtggt ccccattgtg
     2821 ctgtatgccc tcatattgat gcttaaggat gccctggtct ctgcgggaat cgtgcccgaa
     2881 ctgttcggcg agttccgctc ttcccagcgg accatcggga taatagc
//
LOCUS       DAOQQN010000373         2924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_17760_length_2924_cov_6.170791, whole genome shotgun sequence.
ACCESSION   DAOQQN010000373 DAOQQN010000000
VERSION     DAOQQN010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17760_length_2924_cov_6.170791"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLG24_11745"
     CDS             complement(<1..420)
                     /locus_tag="PLG24_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25612.1"
                     /translation="MIGLLSATLILSVIHALIPNHWLPLVAVANAEKWSPRELYLVGF
                     ASAFAHVLGTIVLGILLGLVGIRLSETFESYAHIVAPVVLIVLGLIYFSINLPHSHGT
                     PSGVVVETYRKSKVRWILVFVGLMFLSPCLEVESIFLT"
     gene            839..1999
                     /locus_tag="PLG24_11750"
     CDS             839..1999
                     /locus_tag="PLG24_11750"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HMZ25613.1"
                     /translation="MKFGMVIDTFSTETITVKQGETLINILSKYTIETKDAYNAVEAG
                     RKSFNPRIIHPGLEMTVLKSKKNGQPLYIAFQPNVYKYVVFDLLNHENVKVIDRPVEQ
                     SIEVVSGVMEGSLWETIKKYNMDTDLASQMEDAMECAMDLSRSGKGDMIKLVYEKNII
                     EGEEVDAGKLLAAYYKGRLGEKYVFYFDNGKYKGYFDQDGRPMKKSYLASPLKYAKIT
                     SKFSMSRLHPILNKVVPHFGTDYAAPTGTPILAVSDGVIEAQGYGGGNGNYVKIKHDR
                     TYSTQYLHMSRFPKGQRKGQRVRQGQVIGYVGSTGLATGPHVCFRFWKNGRQVNPKSC
                     KLPAPNPIPGSSLTEFKALRNDMLSKLAAIGKPLPGETLVKKSEDELFLIEP"
     gene            complement(2012..>2924)
                     /locus_tag="PLG24_11755"
     CDS             complement(2012..>2924)
                     /locus_tag="PLG24_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ25614.1"
                     /translation="SAEVLLDASGLEKSYNGVNVFRDVDFRITRGERVAFVGQNGMGK
                     STLAKILIGKEKADKGTVVIGPNTFLGYYAQNQTEVLQGNLTLLETMENESPPERRTQ
                     LRNILGSLMFSGEDVDKKVSVLSGGERGRLALALLLLHPINLLVLDEPTNHLDLKAKE
                     VLKKAIMDYDGTMIVVSHDRDFLSGLTEKTIEFRDNTIKEYLGDVNYFLDKRQMETFR
                     EVEAVQKQTANSQPKNSARVDNSKKQEIEKTEKRINELEAKIKSLHDKMMTDDFYNSP
                     DYQKTTTQYANLQKELEIEMTRWEEML"
BASE COUNT          927 a          611 c          559 g          827 t
ORIGIN      
        1 agttagaaat atggattcta cttcaaggca aggggaaaga aacatcagcc cgacaaaaac
       61 caatatccat ctcactttgc ttttccgata ggtctcaact acaacccctg aaggcgtacc
      121 atgagaatgc ggaaggttaa tcgaaaaata aatcaatcca aggacaatca atacaacggg
      181 tgcaacaata tgagcatagg attcaaacgt ttctgacaaa cggattccaa ccagccccaa
      241 taaaataccg aggacaatgg tgccaagcac atgagcaaaa gcagaggcga atccaacaag
      301 gtataattct ctgggactcc acttttcagc attggcaact gcaaccaacg gaagccaatg
      361 attaggtatc agtgcatgaa ttacactcag aattaatgtt gcagaaagta gtccaatcat
      421 aatcaggcaa aaatacatat gttctcaaca actgattaac attttttaac acctcaaagt
      481 aaaatttaaa ataaataaat acaataacgt gaatattaaa ctgttatagt aattgattta
      541 aaattattca aaacaatatt ttataatttt tatttaatcc ccaataataa attacgtaat
      601 aagtacctga atttaaattg ttttcctatt tttacggctt gaaggatact ttattacatc
      661 attatattaa aagccggtga atactaataa tttgataaag catacaggat tagctttcac
      721 gtttatattg tcatcgctga ttctatcaaa acttctgcca aataatttag acccatctgc
      781 caaatacaat aaaaatatta tttctacctt aagtagtttc aacatcaaat cgcctgaaat
      841 gaaattcggt atggtgattg acactttcag tacggagact atcaccgtta agcaaggaga
      901 aacccttatc aatattcttt caaaatacac gattgaaact aaagatgctt ataatgccgt
      961 tgaagccggc aggaaatcat ttaacccacg aatcattcac cccggacttg agatgacggt
     1021 tttgaagagc aagaaaaacg gacagcctct gtatattgct ttccaaccca acgtatataa
     1081 atatgttgta ttcgatctcc ttaaccatga aaatgtcaaa gtgatcgaca gaccggtaga
     1141 gcaaagtatt gaagttgtat caggtgtcat ggaaggttcc ttatgggaaa ccatcaagaa
     1201 atacaatatg gatacggatc tggccagtca gatggaagat gcgatggaat gtgccatgga
     1261 cctcagccgt tcaggtaaag gtgatatgat caaacttgtt tatgaaaaaa atatcatcga
     1321 aggagaggaa gtagatgccg gcaaactact cgctgcatac tacaaaggca gattgggcga
     1381 aaaatacgtg ttttactttg ataacggtaa atataaagga tatttcgatc aggatggaag
     1441 acctatgaag aaatcatatc tggcatctcc actgaaatat gctaaaataa cctcaaagtt
     1501 ctccatgtcc agacttcatc ccattctcaa caaagtggtc cctcattttg gaacagacta
     1561 tgcagctccg accggaactc ccattctcgc cgtatctgat ggcgtgatcg aagctcaggg
     1621 ctacggtggc ggtaatggta attatgtaaa aataaagcac gatcgtactt attcaacaca
     1681 gtacctgcat atgtcacgct tccctaaggg acagagaaaa ggtcaaaggg taagacaagg
     1741 acaggtaatt gggtatgttg gttcaaccgg tctggctacc ggacctcatg tgtgtttcag
     1801 gttctggaag aatggaagac aggttaatcc aaaaagctgc aaactacctg ctcctaatcc
     1861 gattccgggc tcaagtctga cagaatttaa ggctctacga aatgacatgc tgagtaaatt
     1921 agcagccatc ggaaaaccac ttccgggaga aacattggtc aagaaaagtg aagatgaatt
     1981 attcctgatt gaaccttaat cagggacaat atcatagcat ttcttcccaa cgcgtcattt
     2041 ctatttccag ctctttttgt aggttggcgt attgcgtggt cgtcttttga taatcaggtg
     2101 aattgtagaa atcgtcagtc atcattttat catgaagcga tttgattttt gcctcaagtt
     2161 cattaatgcg cttttcggtt ttctcaatct cctgcttttt actattgtca acacgggcag
     2221 aattctttgg ttgtgaatta gcagtttgct tttgcactgc ttccacttcg cggaatgttt
     2281 ccatctgccg tttgtctaag aaatagttga catcccccag atattcttta atcgtattat
     2341 ccctgaattc tatggtcttt tcggtcaacc cggaaagaaa atcccggtcg tgggagacta
     2401 caatcatcgt gccatcataa tccataatgg cttttttaag cacttcctta gccttcaggt
     2461 caaggtgatt ggtaggttcg tcgagcacaa gcaaattgat ggggtgtaaa aggagtaagg
     2521 ccaatgccaa cctgccacgc tctcctcccg acaaaacgct cacctttttg tccacatctt
     2581 caccggaaaa catgagggaa cctaaaatat tccgaagttg agttcgtctc tccggtggcg
     2641 attcattttc catagtttca agcaaggtta ggttgccttg caggacttca gtttgattct
     2701 gcgcgtaata tccgaggaag gtgttcggcc cgattaccac ggttccctta tctgcctttt
     2761 cttttccaat taatattttg gccaaggtag atttccccat tccattctgt ccaacgaatg
     2821 ctacccgctc acctcttgta atacggaagt ccacatcccg aaatacattg acaccattgt
     2881 atgacttttc caatccggaa gcatccagca aaacttcagc agaa
//
LOCUS       DAOQQN010000374         2901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_17987_length_2901_cov_4.664090, whole genome shotgun sequence.
ACCESSION   DAOQQN010000374 DAOQQN010000000
VERSION     DAOQQN010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17987_length_2901_cov_4.664090"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="PLG24_11760"
     CDS             <1..1344
                     /locus_tag="PLG24_11760"
                     /inference="COORDINATES: protein motif:HMM:NF040143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HMZ25615.1"
                     /translation="DISLAHTLESVNLAQTLANLRSTDIPGIDTIIEACSAVYGLDQK
                     FKTDELLKEGLIGDTLGEVPVNITNQALIVDFERKIKDCKLKKYTQDTKSHDLTLDLR
                     NPVQLEASRLIWQLRLLGITWGKEAKGRTSAKGSFNEHWSLKWKSEFLILLFHQCLYG
                     SSIDQAAAIKTKDELNRKDNVLFYLDILTPVINSQIADLTEFLLKQIENTLFKQEEAE
                     SWMQLIPQLCGYFKYGSVRSLDLSQVKNLIEILVTRLTINFENILISIPEQDLDSTLD
                     TIIRLHHAILQTGEESLTLLWMGHIERLLYTFEIPAIFRGWITGVCLEKKIIDLDEAL
                     GLMTDEMSDRDNLEPSVAWLLGIMSSQVFTAFAVPSIVKIIDLWLADIDYERFREILP
                     GLRKAFSKNSTKLKASFRMHSGKEKPALDTWTREKIIDEDLTKIFSGYIKSFIPT"
     gene            1555..2040
                     /locus_tag="PLG24_11765"
     CDS             1555..2040
                     /locus_tag="PLG24_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25616.1"
                     /translation="MSVSIEKQCQLLDRQLYPAVDEAIDAALMNCSDQDSDPELQEHL
                     FTVLRIRNEFESLSNFERKLVFNTLIRAGKNNPAKNVGPTANIIELIRLIRYKDHKIE
                     GYIQSLEPVSRGKYPCLQKLYDLLSRLFTIEKTKLYDLVDQIIPLNSPVVFDNGSFNN
                     N"
     gene            2042..>2901
                     /locus_tag="PLG24_11770"
     CDS             2042..>2901
                     /locus_tag="PLG24_11770"
                     /inference="COORDINATES: protein motif:HMM:NF023578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HMZ25617.1"
                     /translation="MPKASTTIEKQVSCSHCGTECTGDIRAADAVFCCQGCKIVFGLL
                     NEKGLCDYYEYNEHPGLTIRPIENNSKFDYLNNDEITDKLILFKNNDQSHVRFYLPQM
                     HCSSCLYLLEHLNKLNDGILNVRVDFVKREAFIVFDHNLLALSDLAGLLTSIGYEPHI
                     SLSGDDTEILKKADRKKIYKVGIAGFCFANIMMMSIPSYLAGGVEIERPIALFLRYGM
                     LILSIPVVFYSGAEFFISAWKSARERYLNIDAPIALAIAITFIRSLYEIFLGSGEGYL
                     DSLSGIIFFM"
BASE COUNT          920 a          597 c          583 g          801 t
ORIGIN      
        1 gacataagct tggcacatac ccttgaatca gtcaatctcg cccagacttt ggccaatttg
       61 cgaagtacag atattccggg catcgatacc atcattgaag cttgctcggc agtgtatggt
      121 ttggatcaaa aattcaagac cgacgaacta ctcaaggaag ggctgattgg agatacactg
      181 ggtgaagtac ctgttaatat cacaaatcag gccttaatag tggatttcga acgtaaaatc
      241 aaggactgta agttaaaaaa atacacccag gacactaaaa gtcatgacct tacgctagac
      301 cttcggaatc cggtacagct ggaagcctcc cgcctcatct ggcaactcag actgcttgga
      361 ataacctggg gcaaagaagc caagggacga acctctgcaa agggaagttt taatgaacac
      421 tggagtctga aatggaagtc tgaattcctc atcctgctct tccatcaatg cctgtatgga
      481 tccagcatcg atcaggcagc tgccatcaaa accaaagatg agctaaacag gaaggacaac
      541 gtattatttt acctggatat ccttacgcct gtcatcaact cccagatcgc cgacctaacg
      601 gaatttttac tcaaacaaat agaaaacaca ctattcaaac aagaagaggc tgagtcatgg
      661 atgcaactca tccctcaatt gtgtggctac ttcaaatatg gttcagttcg ctctctggac
      721 cttagtcagg tgaaaaatct gatagaaatt ttagtcacca gactgaccat caactttgaa
      781 aatatcctga taagtattcc ggaacaagat ttggacagta ccctggacac cattatacga
      841 ctccatcatg ccattcttca aaccggagag gaaagcctga ctctcctatg gatgggacat
      901 atagaaaggc tactttatac ctttgagata ccggcaatct tcaggggctg gatcacaggt
      961 gtctgtctgg aaaagaagat aatcgacctg gacgaagcac ttggtctgat gacagacgaa
     1021 atgagtgatc gggataatct cgaaccgagt gtcgcctggt tactgggcat catgagcagt
     1081 caggttttta cagcctttgc ggtaccttcc attgtgaaaa tcattgattt gtggcttgca
     1141 gatatagatt atgagagatt cagagagatc ctgccgggac ttcgaaaagc tttttcaaaa
     1201 aactcgacca agctaaaggc cagcttcagg atgcattccg gcaaggagaa acctgcattg
     1261 gacacatgga caagagaaaa aatcatagat gaagacttga caaaaatatt ttcaggctat
     1321 atcaaatcat tcatacctac ataaaatatt acatttaatt gtttttatta atttcagttt
     1381 tttaaatatt gcttaaatcc agggccaatc tattgaatat tatttatatc ttagccctat
     1441 atataaatac aatataaaat atgacttttg tcatgtttta tcctttctta agtcattgtt
     1501 tacttaaaag gcgcgaagca tctttgtaca ttcatttgac tgaaaatcgt tgcaatgtcg
     1561 gtatctatag aaaaacagtg ccaattactt gaccgtcaat tatatccggc tgtggatgaa
     1621 gcaattgatg cagcattgat gaattgttcg gatcaggatt ccgatcctga attacaggaa
     1681 catttattta ctgtcctgcg aatcagaaat gaattcgaaa gtctatccaa cttcgaaaga
     1741 aaactggttt tcaataccct aatcagggca ggtaaaaata atccggcaaa aaatgttggt
     1801 cctaccgcaa atatcattga attgatccgg ctaatcaggt acaaggatca taaaattgaa
     1861 ggatatatcc aatcactaga accggtatca agaggtaagt atccgtgttt gcaaaaacta
     1921 tatgatttat taagccggct ttttaccatt gaaaaaacga agctatacga ccttgtagat
     1981 caaatcattc ctctgaattc tccggttgtt ttcgataacg gttcatttaa caacaactga
     2041 catgccaaaa gcaagtacaa caatagagaa acaagtcagc tgttctcact gtggaactga
     2101 atgcaccgga gatataaggg cagccgatgc tgttttctgt tgtcagggct gtaaaatagt
     2161 gtttggactg ctcaacgaaa aaggtttgtg tgactattat gaatacaatg agcaccccgg
     2221 tctaaccatc cgccctatag aaaacaattc aaaattcgat tacctaaaca atgatgaaat
     2281 aacagataag ttaatcctat ttaagaataa tgatcaaagt cacgtcaggt tttatctgcc
     2341 ccagatgcac tgcagtagtt gtctctatct tttggagcat cttaacaaac tgaatgatgg
     2401 catcctcaat gtgcgggtgg actttgttaa gagagaagct ttcattgtat ttgaccataa
     2461 cctcttagca ttatctgacc tggcaggatt actgacatct atcggttatg aacctcatat
     2521 cagcttgtca ggtgacgata cagaaatcct gaagaaggca gaccgtaaaa aaatttacaa
     2581 agttggtata gcgggattct gctttgccaa catcatgatg atgagcattc catcctatct
     2641 ggcaggaggt gtagaaatcg agcggcccat agcacttttc ctccgatatg gaatgcttat
     2701 actttccatc ccggtcgtat tctactcagg ggcagaattt tttatatcag cctggaaatc
     2761 agccagggaa agatatctga atatcgatgc acctatagca ctggcaatag ccataacctt
     2821 tatcagaagc ctgtacgaga tattcctggg ctcgggcgaa ggttatctag acagcctatc
     2881 aggcatcatt ttcttcatgt t
//
LOCUS       DAOQQN010000375         2852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_18490_length_2852_cov_3.852699, whole genome shotgun sequence.
ACCESSION   DAOQQN010000375 DAOQQN010000000
VERSION     DAOQQN010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18490_length_2852_cov_3.852699"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..568
                     /gene="rpsD"
                     /locus_tag="PLG24_11775"
     CDS             11..568
                     /gene="rpsD"
                     /locus_tag="PLG24_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HMZ25618.1"
                     /translation="MYGPDKAFDKKKYAPGQHGASRKRRQKSDYALQLMEKQKAKYTY
                     GVLERQFRNTFTKAARKGGVTGTVLLQFLESRLDNVVYRMGIASTRREARQLVSHRHV
                     VVNGVVTNIPSFHVRPGDVVEVKGKSRGMDIILSNVKSKHDHRKYAWIEFNPERMEGK
                     FLAYPEREQIPENINEQLIVELYSK"
     gene            618..1616
                     /locus_tag="PLG24_11780"
     CDS             618..1616
                     /locus_tag="PLG24_11780"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HMZ25619.1"
                     /translation="MSILNFQKPDKIILQKSTDFDGVFEFKPLEPGFGQTIGNSLRRV
                     LLNSLEGFAISAIRIGGVDHEFSTIPGVVEDVIEIILNLKQVRLKLQMKDEDIQNEKI
                     YLTLRGKEQFLSGDIEQFTNVFKVMNPDLLICQMEPSVTLEIELTITKGRGYVAADEQ
                     PERESVIGVIPVDSIYTPVKNVAYQVSNTRVGQRTDYEKLTIEVKTDGTIHPEDAVKE
                     ASRILIQHLMLITDENISFDDAAKGEEDVVDEHILHMRKLLKTSLEDLDLSVRAYNCL
                     KAAKINSLGEMVQYETHELLKFRNFGKKSLVEIEELLSTKGLHFGMDLSKYKLEEE"
     gene            1685..2323
                     /gene="rplQ"
                     /locus_tag="PLG24_11785"
     CDS             1685..2323
                     /gene="rplQ"
                     /locus_tag="PLG24_11785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HMZ25620.1"
                     /translation="MRHGNKVNALGRTKSHRDAVMKNMASSLIEHKRINTTLAKAKAL
                     RGYLEPLITKSKNNNTHSRRVVFSYLQNKSAVDTLFSDIREKIGDRPGGYLRIIKLGG
                     RSHDNAEMAMIEFVDYNNIYNPNESLDGKAKSKTRRSRKKSSGVKQAESAAPTEELVE
                     ITENPVEEAPVAVDVVETAQTEEEVVTAETTEAPVEPADETPAPDAEENKEV"
     gene            2436..>2852
                     /locus_tag="PLG24_11790"
     CDS             2436..>2852
                     /locus_tag="PLG24_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HMZ25621.1"
                     /translation="MINLLSDTVTKPDRPMLEAMMAAEVGDDVFGEDPTVNALEERMA
                     GTFGMEAALFCPSGTMSNQIAIKVYTRPLDELICDHYSHVFLYEMGGYAFNSGIAVNP
                     LAGRFGKLTAGQIEQAIKPAEDWLPRTKLVVLENSTN"
BASE COUNT          874 a          537 c          680 g          761 t
ORIGIN      
        1 tggtgaggct atttacgggc cggataaggc ctttgataag aaaaaatatg ctcccggcca
       61 gcatggtgct tccagaaaga gaaggcaaaa gtctgattat gcacttcaat tgatggaaaa
      121 acaaaaggca aaatatacct atggtgtatt ggagcgtcag tttagaaata cattcaccaa
      181 agctgccaga aaaggcggtg tgaccggtac cgtgttacta caattccttg aatccagact
      241 ggacaatgtt gtttacagga tgggaatcgc cagtacacgt cgggaagctc gtcagttggt
      301 ctcacataga catgttgtag ttaatggcgt tgtaacaaat attccatcct tccatgtcag
      361 acccggagat gttgtcgagg taaaaggtaa atccagaggt atggatatca tcctttccaa
      421 tgtcaaatcc aagcatgacc accgtaaata tgcctggatt gaattcaatc ctgaacgtat
      481 ggagggaaag ttcctggctt atcctgaaag agagcaaatt cctgaaaaca tcaatgaaca
      541 gttgatcgtc gaattgtatt ccaagtaatt ttgtattctt ttatcaagtc ttttcttcat
      601 ctatagattt tttcaaaatg agtatattaa attttcaaaa acctgataaa attatacttc
      661 aaaaatccac tgacttcgat ggtgtttttg aatttaaacc gttggaaccg ggcttcggtc
      721 agacaatcgg taactcattg agaagagtac tcctcaactc tctggaaggt ttcgctattt
      781 cagcaatcag aattggcggt gttgaccacg aattttcaac cattcccgga gttgtggaag
      841 atgtcattga gatcatcctg aatcttaagc aggtccgttt gaagctgcaa atgaaggatg
      901 aagatattca aaacgaaaaa atttacctca ctctccgcgg taaggaacaa ttcctttccg
      961 gtgacattga acagtttacc aatgtgttca aagtgatgaa cccggatctg cttatttgtc
     1021 agatggaacc cagtgttaca ttggagattg aattgaccat cacaaaaggc cggggatatg
     1081 tcgccgcgga cgaacaacca gagcgtgaaa gtgtcatagg tgtaattcct gtggattcta
     1141 tttatactcc ggttaagaat gtcgcttacc aggtttccaa tacaagggta ggacaacgta
     1201 cagactatga gaagttgacc atcgaggtga agacagatgg gacaatacat ccggaggatg
     1261 cagtgaagga ggcttcaagg attttgatcc agcatctgat gctcatcact gatgaaaata
     1321 tttcatttga tgatgctgct aaaggtgagg aagatgttgt cgatgaacat atcctgcaca
     1381 tgcgtaagtt gttgaagact tctctggagg atctggatct gtcggtaaga gcttacaact
     1441 gtctgaaagc tgccaaaatc aattccctgg gtgaaatggt acaatatgaa actcatgaat
     1501 tactgaagtt ccgcaatttt ggtaagaagt ctttggtcga gatcgaagaa ttactttcta
     1561 caaaaggact gcactttggt atggaccttt ccaaatacaa gctggaagaa gaataattta
     1621 atctataaaa ttgcaaatta ccggtgccta ttgcccggtg ttgcgaagct aaacatttga
     1681 aaaaatgaga cacggtaaca aagtcaatgc ccttgggcgt actaaatcac atcgggatgc
     1741 agtaatgaaa aacatggcat cttccctgat cgagcataag aggattaaca ctacattggc
     1801 caaggcaaag gctttgagag gttacctcga accgcttatc actaaatcta agaataataa
     1861 tacacactcc cgcagggtag tgttttcgta ccttcagaat aaaagtgctg tcgatacact
     1921 tttctctgat atcagggaga aaattggcga cagaccgggt ggttatctca gaattatcaa
     1981 actaggaggt agaagtcacg ataatgccga aatggcgatg attgaattcg ttgattataa
     2041 caacatttat aatccgaatg aatctcttga tggaaaggcg aagagtaaaa caagaagaag
     2101 ccgtaagaag tcttccggtg tcaaacaagc ggaatcggct gctccgaccg aggagttggt
     2161 agaaattact gaaaatccgg ttgaggaagc tccggtagcg gttgatgttg ttgaaacagc
     2221 gcaaacagaa gaggaagttg ttacagcaga aactacggaa gcgcctgttg agccagcgga
     2281 cgaaactccc gctccggatg ctgaagagaa taaggaagtg tgattaagat aacatattga
     2341 ttgaaaagcc cggatattcc gggctttttt tttgttgccc gttgagagtt gaaaacagtt
     2401 ttgtaatttc ggttttataa atgggaaata gacaaatgat caatttactt agtgataccg
     2461 taacgaagcc ggacaggcca atgctcgaag cgatgatggc ggctgaagtc ggtgatgatg
     2521 tctttggtga agacccaacg gtcaatgcac tggaggagag gatggcagga acttttggca
     2581 tggaagctgc tttgttttgc ccgtccggga ctatgtccaa tcagatagct attaaggtgt
     2641 atacacgccc cttggacgaa ttgatttgcg atcactattc tcatgttttt ctctacgaaa
     2701 tgggcggtta cgctttcaac agtggaattg cggtcaaccc ccttgccgga cgattcggaa
     2761 agttgactgc cgggcagatc gaacaggcaa tcaagccggc tgaggattgg cttccaagga
     2821 ctaaattggt cgtactggag aattctacga at
//
LOCUS       DAOQQN010000376         2846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_18549_length_2846_cov_5.084558, whole genome shotgun sequence.
ACCESSION   DAOQQN010000376 DAOQQN010000000
VERSION     DAOQQN010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18549_length_2846_cov_5.084558"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /gene="meaB"
                     /locus_tag="PLG24_11795"
     CDS             complement(<1..955)
                     /gene="meaB"
                     /locus_tag="PLG24_11795"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HMZ25622.1"
                     /translation="MFDPEIQEIFSKIKERNKVFLARAITWAESSNETSRRKILELLD
                     HLGDFRSNAIRIGVTGPPGVGKSTLIESLGLELIQKGYSVAVLAVDPSSSESHGSILG
                     DKTRMTKLSSHPSAYVRPSPSGLELGGVNQASRESILICEAAGYDFIFVETVGVGQSE
                     IEVKEMTDIFLLLLNPGGGDELQGIKKGIMEAADIIAITKYDSHLTKAGEEAFSHVKA
                     SLQVMKNYRLELPDVLNVSSVTSHNTERLLKIIESKTAAQKQTGEWDVKRKNQERVWF
                     RRGLLRRLNELIENDPTTLTILDNYEKKTNNATRPVPIDIEK"
     gene            complement(1129..>2846)
                     /locus_tag="PLG24_11800"
     CDS             complement(1129..>2846)
                     /locus_tag="PLG24_11800"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ25623.1"
                     /translation="YIAPFKDTLICYGDKLSLGDFSKAKLYEFIPPDDIDFSDFNHPF
                     IIAKKDTRHTVKITSTDQKCQFEHEFIIKVNEGRFELKTPDYEELCKGDSTRIAWSFI
                     PKNSKVTWTPNDGSITTLNDSSMTVKPLKTTIYTGTFTVGPCTYTKKVEVRVDSLPDL
                     PLDHQPVRPYYCRGEIVSLFSPQYDKTLYPDMKFDWGNALGAIEPIDKQNLAISTQDT
                     FLYIRKTTNHACVRFDSLQIDVKIPKIQFSLLDTLVCENEPVKVNVETDMTDLEWKPE
                     EGVTCSNDCKTAIIKTANTTTYTLTGKADGCPGAASMTIRVKKPLIGLTVTDTTICPN
                     EPVTVSVTTDATNISWSPGEKTSCNNCATTTLTTPTEQNFVVGGIQDGCPAWGGCIIR
                     IRPPYQLEISVSPPDNVAIGNPVTASVVNPIPGASYQWKINGRDLGVHGSSYEIVLES
                     KNDRIEALLVPGGNNYCFGSGEIFVAGVTPYIYVPNSFTPNGDSKNDVFRAVVPDGVT
                     VLEMTIVNRWGQRVFTETNTNNGWDGRFNGKEAPSDTYVFIVKYQLLQGGVIDSRRGE
                     VTLYR"
BASE COUNT          761 a          606 c          568 g          911 t
ORIGIN      
        1 gtttttcaat gtcgatggga actggtcggg ttgcattatt tgttttcttc tcataattgt
       61 caagaatggt taacgtagtg ggatcatttt ctatcaattc atttaatctg cgtagcaatc
      121 ctcttctgaa ccaaactcgt tcctggtttt ttcgtttgac atcccattcg cctgtttgtt
      181 tttgagcagc cgttttgctt tcgataattt taagcaatct ttcggtgttg tgacttgtga
      241 ccgaggaaac attgaggaca tccggtaact caagtcggta gtttttcata acttgcaacg
      301 atgccttaac atgactgaaa gcctcttcac ccgctttggt caggtgagaa tcatatttgg
      361 tgattgctat aatatcagca gcttccataa tgcctttttt tattccctga agttcatcac
      421 ctccacccgg attgagcaat agcaggaaga tgtcagtcat ttcctttacc tcaatttcgg
      481 actgccctac acctacggtt tcaacaaata taaaatcata acctgctgct tcacatatca
      541 atatactttc ccgacttgcc tgattgacac ctcccagttc gagtccggat gggctgggcc
      601 tgacataagc agatggatgt gaagaaagct tagtcatccg cgttttatcc ccaagtatac
      661 tcccatggct ttcgctgctg gatgggtcga cggcaagaac tgctacagaa tacccttttt
      721 gtataagttc aagacccaat gattctatca gggtactttt tcctacacct ggaggccccg
      781 tcactccgat cctgattgcg tttgatctga aatctcctaa atgatcgagg agttcaagta
      841 tcttccggcg ggaagtctca ttactggatt ctgcccaggt aatagccctg gcaagaaaga
      901 ctttatttct ttcttttatc ttgctgaaaa tttcctgtat ttccgggtca aacaaggtta
      961 taccgtttag tttttataag agatataaaa aataataaaa tataatagtg ttaaaatcaa
     1021 tagaatagct aaataatctt accgatataa tattggacaa gaggaataat cgacatttta
     1081 atcagtagtt attccgcctt atatgaaatt acataatcag ccccaatatc aacgatacaa
     1141 tgttacttcg ccacgccttg aatcaatgac gcctccctgc aacaactggt atttgactat
     1201 aaatacgtat gtatcacttg gtgcttcttt tccgttgaat ctaccatccc atccattgtt
     1261 tgtattggtt tcagtgaata ctctctgacc ccaacgattg acgattgtca tctcaaggac
     1321 agtgactcca tcgggaacga cagctctaaa tacatcattc tttgagtcac cattaggcgt
     1381 aaaagagttt ggaacataga tgtaaggagt tactcctgca acgaaaatct cacctgaacc
     1441 aaaacaatag ttattaccac cagggacaag taaagcttct atacggtcat ttttcgattc
     1501 caggacaatt tcataggaac ttccatgaac gccaagatct ctgccattga ttttccattg
     1561 ataggaagca cctggaattg gattgacgac gcttgccgtg acgggattac caatagctac
     1621 attgtcaggc ggagatacag atatctccaa ttggtatgga ggtctgatcc tgatgataca
     1681 acctccccag gcagggcatc catcctgaat tcctcctaca acaaagttct gttcggtagg
     1741 agtggtgagt gttgttgtag cacaattgtt gcaactggtc ttttctccgg gtgaccagga
     1801 gatattagtg gcatccgtgg tcacacttac agtgacaggt tcattcgggc agattgtcgt
     1861 atcagtaact gtaagaccaa tcaatggttt cttgaccctg attgtcatag atgcagcacc
     1921 cgggcaaccg tccgctttac ctgtcagagt gtaagtagtt gtattggctg ttttgatgat
     1981 agctgtcttg caatcattgc tacaggttac gccctcttcc ggcttccact cgagatcggt
     2041 catgtcggtt tcaacattta ccttaacagg ctcgttttca cacaccaatg tgtctaaaag
     2101 gctgaattgg attttaggaa tcttcacatc tatttgcaac gaatcaaaac gaacacaagc
     2161 atggttggtt gttttgcgga tgtataagaa cgtatcttga gtgcttatag ccaggttttg
     2221 cttatctatc ggttcaatcg ctcctaatgc attgccccag tcaaacttca tatccggata
     2281 aagtgttttg tcgtattgcg gtgaaaataa tgacactatt tctcctctgc aatagtatgg
     2341 cctgacaggt tgatgatcca atggtaaatc agggaggctg tccactctga cttctacttt
     2401 cttagtatag gtacatggac caacggtaaa ggtcccggta tagatcgttg tcttcagtgg
     2461 tttgactgtc atggagctgt cattgagggt tgtgatacta ccgtcatttg gagtccaggt
     2521 aaccttactg tttttgggaa tgaatgacca tgcaattctt gtactatcac ctttgcataa
     2581 ttcttcatag tcaggagtct ttaactcaaa tcttccttca ttgaccttaa taataaattc
     2641 gtgttcgaat tggcactttt gatcggtgga ggttattttg actgtatgtc tggtatcttt
     2701 ttttgcaata atgaatggat gattaaaatc cgaaaaatca atatcgtccg gcggaataaa
     2761 ttcgtataat tttgccttgc taaagtcccc aagagataat ttatcaccat aacagatcag
     2821 agtgtcctta aaaggtgcaa tatatt
//
LOCUS       DAOQQN010000377         2840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_18611_length_2840_cov_5.671454, whole genome shotgun sequence.
ACCESSION   DAOQQN010000377 DAOQQN010000000
VERSION     DAOQQN010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18611_length_2840_cov_5.671454"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PLG24_11805"
     CDS             <1..463
                     /locus_tag="PLG24_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25624.1"
                     /translation="YCTGGIRCEKASAYMKHKGYKNVFQLDGGIIEYTRQVKDQGLEN
                     KFIGTNFVFDERLGERISDHIIAHCHQCGAPCDTHTNCANDFCHLLFIQCEACRQKYE
                     GCCSPLCKDYLHKPQEERLAAGKAGIFNGSKFGKGRYNPKQIGKNELLKEK"
     gene            555..1007
                     /locus_tag="PLG24_11810"
     CDS             555..1007
                     /locus_tag="PLG24_11810"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HMZ25625.1"
                     /translation="MEYKLASFTNRFAALIIDGILIHVAAKFLTFPFGFGFNPWNVFN
                     GNFLDFAEYSGYVGVIALVYYVFMESSSRSATIGKQVMQIKVLDENGDNLTLSKSIIR
                     NFVKVLCGNIFMLGFFFALFTKNKQALHDILGTTLVVEDRPVAADSPS"
     gene            1472..>2840
                     /locus_tag="PLG24_11815"
     CDS             1472..>2840
                     /locus_tag="PLG24_11815"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25626.1"
                     /translation="MQLTIRNFYLSSIPAGILIRLLGPVLCLFFSVFISIGQPVSTDR
                     AREVSEYTDTLGRTDTTHNDLLADLFDNDTVIVTYMRYGDLFTLLPFEDSLLSLSLRQ
                     MDVARSQNVEYFTLGNIGSPAYSPLVRPVTRGAFDMGNHGYDLYRITFDNFRFYRPAT
                     PYSHVRYGTTTGIRDDNIFTGKLSRNFAKKLTVNVDYSRFNQVGEFLRDRMRNTTFGV
                     GFEQSHWNGRYKTHLIYIYNQFLRDENGGVAEYLTDITGISGKRLSMPVNISNGGSRY
                     REWGVLLNNDLTLLRSRDSLSASSGKGLHIGYEVSYHRTAHTFYDTDVRTKEDSTYYR
                     LFNTDTRGIRNSYIHQVFLNRAYISAEGKTPTTTGVTSDYLRGGIALERNFLTYLPTD
                     SAFTLLKLEGAGHWNLAKVAGVSAKGYYLLSSLTPVFDVEGTLSGKLIKWLHARAWIR
                     LSNLPP"
BASE COUNT          824 a          649 c          607 g          760 t
ORIGIN      
        1 gtattgtacc ggtggaattc gctgtgaaaa agcaagcgct tatatgaaac acaagggata
       61 taaaaatgta ttccagcttg atggcggtat cattgaatat acacgacaag tcaaagacca
      121 gggactcgaa aataaattca tcggaaccaa ttttgttttt gacgaacggc ttggagaaag
      181 aatcagcgac cacatcatcg cccactgcca tcaatgtggc gcaccatgtg atacccatac
      241 caactgtgcc aatgacttct gccacctgct ttttatccaa tgtgaagcat gccggcaaaa
      301 atatgaaggc tgttgctccc ctctctgcaa agattatctg cataaaccac aggaagaaag
      361 gctggctgcc ggaaaagcag gaatcttcaa tgggtcaaaa ttcggaaaag gccgttacaa
      421 ccccaaacaa atcggaaaaa atgaactgtt aaaagaaaaa taaacgtttt tggtcgatac
      481 ttctgtttat tggaaactat tatgtattgc aaagtagtta aaagagtata tttttacctt
      541 aaaaaaacga aaaaatggaa tacaaattgg catcttttac aaatcgattt gctgctttga
      601 ttattgacgg aatactgatc cacgtagccg caaaatttct gacattccca tttgggtttg
      661 gattcaatcc ctggaatgta ttcaatggca acttcctgga ttttgcagaa tattcaggct
      721 atgtgggagt tattgcctta gtatattatg tgtttatgga atcttcctct cgcagtgcca
      781 ccattggcaa acaggtgatg caaatcaaag tccttgatga gaacggagac aacctgacct
      841 tgtcaaaatc aattatcaga aacttcgtca aggtattatg tggcaatatt tttatgctgg
      901 gatttttctt tgcgttattc accaaaaaca aacaggcgct gcatgatatt cttggcacaa
      961 cattggtggt ggaagacaga ccagttgcgg ctgattctcc atcctaaggc gacacgtatc
     1021 taagaagaac agagatgggc tcattggcac ttcaccacca atgggctcat tcctgtaacc
     1081 catctctatt gcgctccgat gatttcaaac aaagggtcaa ggctattctg acctcattct
     1141 ggagactgaa attatctata atatgggtgt gttgtattga aataataacc ggaattataa
     1201 aacatcttga gtgaagggaa tggttacaaa atcatgtaac tgtagttcct accaatcctt
     1261 ccgctaacta atttttgtgt ttgatccggt ttgttatcct gtccacaatc tttttatcat
     1321 ccgggtaagc gacatctaat acaggcgaag aggacatgat tccaacagaa actatatccg
     1381 ttaaaaccaa atgatcacta atcttggttt attttttata aattataatt gcaaagccat
     1441 aagtttgcac cttgtaatga tgccggaccc tatgcagctt accattcgca atttttattt
     1501 aagctcgatc ccggccggga tcttgatccg gttgttgggc ccggtgctct gtctcttttt
     1561 cagtgttttc atatcaatcg gccaacctgt ctccaccgac agagcaagag aggtttctga
     1621 atatacagat actctcggga gaacggatac cacacacaat gacttattgg ccgacctctt
     1681 cgacaacgac actgtcatcg ttacttatat gagatatggc gacttattca ctctgctacc
     1741 cttcgaagac agtttgctct cactatcttt acgacagatg gatgtggcaa gaagccagaa
     1801 tgtggaatat tttaccctgg gcaatatcgg ctcgccggca tacagcccct tagtccgacc
     1861 ggtgacaagg ggagccttcg atatggggaa tcacggctat gatctctaca gaatcacttt
     1921 cgacaacttc cgtttctaca ggcctgcgac gccgtactcc catgtgcgat atgggacaac
     1981 aaccggaatc cgtgacgata atatctttac cggcaaactg agccgcaatt ttgccaagaa
     2041 acttaccgtc aatgtagact attcccggtt caaccaggta ggagaatttc ttcgtgaccg
     2101 catgcgaaac actaccttcg gagtaggatt tgagcaaagc cattggaacg gccggtacaa
     2161 gacacatctg atatacattt acaaccaatt tctaagagat gaaaacggtg gtgtggcgga
     2221 gtatctcacc gatatcacag gcatatccgg caagagactc agcatgcctg tcaatatcag
     2281 caatggcggt tcacgatacc gggaatgggg cgtcctgctc aacaatgacc ttaccttgct
     2341 tagaagcagg gatagcctgt ctgcatcttc cggcaaaggg ctgcacatcg ggtacgaagt
     2401 gagttaccat cgcaccgccc atactttcta cgatacagac gtccggacaa aagaggattc
     2461 aacctactac cggctattca ataccgacac acgaggcatc agaaacagtt atatccatca
     2521 ggtcttcctc aaccgagcct atatttctgc ggaaggaaag acaccgacta ccacaggcgt
     2581 aacatcggat tatctgcgcg gcggaatagc ccttgagcgt aattttctaa cctatcttcc
     2641 tacagactcg gctttcacgt tactcaagct ggaaggagcc ggacactgga acctggccaa
     2701 ggtagccgga gtcagcgcca aaggttacta cttgcttagt tcgctgactc ctgttttcga
     2761 tgtcgagggt acgctttcag gcaagttgat caaatggttg catgcaaggg catggatcag
     2821 actcagcaat ctgccgccat
//
LOCUS       DAOQQN010000378         2823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_18776_length_2823_cov_5.205564, whole genome shotgun sequence.
ACCESSION   DAOQQN010000378 DAOQQN010000000
VERSION     DAOQQN010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18776_length_2823_cov_5.205564"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..>2823)
                     /locus_tag="PLG24_11820"
     CDS             complement(4..>2823)
                     /locus_tag="PLG24_11820"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ25627.1"
                     /translation="PSFAPCNTNSCHTDTPDLPDDNSNYMDYSSCSPVHFTAGQKTRI
                     LSGLELGRPSLLTSIGCSPVLSLDVGVRMSIDQKCKTKFCPIIELVNFGSTTITSGTF
                     EIKLTPGSTFTINWTGNLSANNSISFPADCIDLAPGTYSATVHALTVNGSADENSAND
                     YFTMSTVQVQNVIDVDFDYYNAFQLRYYFRNNSKSFESCKWEFGDGTTSTETSPFHDF
                     VNQGQYTITLTCYNSCGDSTKITKTIEIQACSGQLRGRVQSLNDYIEPDEDICEIVQN
                     GILYLRPELADIGYYWTFFSICDTICTGEGYTIKIRLKNDNASGGINAYDTGLEVIGE
                     GENAGIFIMGLPWAIDFTRVWVGNNYLTGLPQLIMKLGDWKTIEMELKNNVLSYKYEG
                     SNFFQMEYPGKVCNIHNINLTFKGSGSVDWIQVYDVNGNLVYEENFDDCNNLTKSIPC
                     PKRPINAEVLVTDECRQPKVEIKNLNGSDDIITTQVFPGVPVDANGRYELVNPGSYKF
                     LLTRTCPLADSSFVLEVVPPMKDSLIRFEQPLCNGSYGFINVSGVGGRPPYEFNINGK
                     QSPTGVFHDLAPGNYEVIITDQNGCSVKNNYTIQDPATPLSFAVDSFRYDLTCYNPTS
                     YIQLGVSPATLNVYFTLDDSLQTNHGYFENMGAGMHKISIHDESRCYNDTLYFEVRDL
                     RKTTHTDLTYDICPGDSVEVAGNFYSQVGTYNDTLVAYTGCDSILAISIHEYVKANAG
                     HSLGIPGYCNNVEQVIKLDELIAGEDKDGFWKVIQGDNNTFDSDNSTFNSFKASPGSY
                     IFQYKVNGAKDCVSDSVMIELDVFDGPKSILFSNDSLFNCRDIVALSAIPDIQSSDIA
                     SVQWYLGDKQIEGANGLQTIISDPRSGIYRVRLVDKNGCELEARQHIRIEYSLPYFAP
                     NVFSPNFDGINDVFNPSCALFNKLC"
BASE COUNT          794 a          622 c          575 g          832 t
ORIGIN      
        1 tttttaacat aatttgttga ataatgcgca acttgggtta aacacgtcat tgatgccgtc
       61 aaaattcggt gagaagacat tcggtgcgaa gtatggaagg gaatattcta ttctaatatg
      121 ttgcctggcc tccagttcac atccgttttt atcaaccagc ctgacccgat aaatacctga
      181 tcggggatca cttattattg tttgaagccc attcgctcct tctatttgct tatctcctaa
      241 gtaccattgg acagacgcaa tatccgaaga ctggatatcc ggaatagctg acagtgcgac
      301 tatatcccga caattgaaaa gggaatcatt tgaaaatagg atgctctttg gcccatcaaa
      361 tacatccagc tcaatcatga cactgtcact tacacagtcc ttcgccccat tgaccttata
      421 ctggaaaata taactccccg gagatgcttt aaatgaatta aaagtgctgt tgtctgagtc
      481 aaaagtattg ttgtctccct gtatcacctt ccaaaatcca tccttatcct ctcctgcaat
      541 taattcatcc agtttgatta cttgttcaac attattgcaa tatcccggta ttcccaaaga
      601 atgtccggca ttcgccttta catattcatg aatactgatc gccagaatac tgtcacatcc
      661 ggtatatgct accagggtgt cattataggt gccaacctga ctatagaagt taccggcaac
      721 ctcaacagag tcacccgggc atatatcgta ggtcagatct gtgtgtgtgg tctttctgag
      781 atctctgacc tcaaagtaaa gagtatcgtt ataacatcga gactcgtcat ggattgagat
      841 tttatgcata cctgcaccca tgttttcaaa atacccgtgg ttggtctgca ggctatcatc
      901 cagggtaaaa taaacattca gtgttgcagg agaaacgcca agctgtatgt atgaagtcgg
      961 attataacaa gtcaaatcat acctgaagct atccacagca aaggagagag gtgttgccgg
     1021 atcctgtatg gtataattgt ttttgacaga acatccgttt tgatccgtta ttataacttc
     1081 ataatttccg ggagccagat catgaaagac accggttgga ctttgcttac cattgatgtt
     1141 gaattcatag ggtggccttc caccgactcc tgaaacattg atgaagccat aagatccgtt
     1201 acacaagggt tgctcaaaac gaatcagcga atccttcatg ggtggcacta cctccaggac
     1261 aaatgaggaa tctgccagcg gacatgttct cgtaagtaaa aatttatagg agcccggatt
     1321 aaccagttca taccggccat tggcgtcgac aggtacacca gggaacactt gggttgttat
     1381 tatgtcatct gacccattca ggtttttgat ttcaactttt ggctgccggc attcatccgt
     1441 tacaaggact tcggcattga tgggcctctt gggacatgga atagactttg tcagattatt
     1501 gcaatcatca aaattctctt catacaccag atttccgttt acatcatata cctgtatcca
     1561 atccacggaa ccggaccctt tgaaggtcaa attgatgtta tgtatattac acaccttccc
     1621 cggatattcc atctggaaga aattgcttcc ttcgtattta taactcagca cgttattttt
     1681 caactccatc tcaatcgttt tccaatcacc tagtttcata atcaattgag gtaaacctgt
     1741 caggtaatta ttgcctaccc agactctggt aaaatcaatc gcccagggta gacccatgat
     1801 aaaaatccct gcattttcac cttcgccaat gacttccagc ccagtatcat atgcattgat
     1861 tccaccggaa gcattatcgt ttttaagtcg gatctttatg gtatagcctt cccctgtaca
     1921 gatggtatca cagatagaaa aaaaggtcca ataataacct atgtcggcaa gttcaggtct
     1981 aagatacaag ataccattct gtactatttc acaaatatct tcatccggtt caatgtaatc
     2041 gttaaggctt tgtactcttc ctcgtaactg acctgagcaa gcttgaattt caatggtctt
     2101 ggtaattttt gttgaatcgc cacaagagtt gtagcaagta agggtgattg tgtattgacc
     2161 ctgatttaca aaatcatgga agggactcgt ctccgtactt gtagttccgt caccgaactc
     2221 ccatttgcac gactcaaaag acttagaatt atttcggaaa taatatctga gctggaaggc
     2281 attatagtag tcaaagtcaa catcaatgac attctgaacc tggaccgttg acatggtaaa
     2341 atagtcattg gcgctgtttt catcggctga tccattaacg gtaagagcat gtacagtggc
     2401 actgtaagtt cccggagcaa gatctataca atctgcaggg aatgagatgc tattatttgc
     2461 tgagagattg cccgtccaat tgatggtgaa tgtgctgccc ggtgtcaatt tgatctcaaa
     2521 ggttccggaa gtgatcgtgg tcgatccaaa atttaccaac tcgatgatag gacaaaattt
     2581 ggttttgcat ttttggtcaa tgctcattct gacaccaacg tctaagctaa gtaccgggct
     2641 gcaaccaatg gaagtcaaca ggctgggcct gcccagttcc aaccctgata ggattctggt
     2701 cttctgaccc gctgtaaaat gtaccggtga acaggacgaa taatccatat agttggaatt
     2761 gtcatcggga aggtcaggtg tatctgtatg gcaggagttg gtattacagg gtgcaaacga
     2821 ggg
//
LOCUS       DAOQQN010000379         2807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_18937_length_2807_cov_5.798328, whole genome shotgun sequence.
ACCESSION   DAOQQN010000379 DAOQQN010000000
VERSION     DAOQQN010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18937_length_2807_cov_5.798328"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1493
                     /locus_tag="PLG24_11825"
     CDS             <1..1493
                     /locus_tag="PLG24_11825"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMZ25628.1"
                     /translation="AVKNASLAQSPLILLGGAAATILKGRGSLQDIDQLSIMKTVCKW
                     SASISRVKDIVPTLEKAFQIAQEGVPGPVFVECPIDVLYGEELVRDWYGAKSNQEPAK
                     NFREKALRWYVNFHANRLFRGKDSVDFDKPVVAPIFPIHGTMDVSKAAEMLRKAKKPL
                     LIIGSGAMMQAARAGDLAQAVERLGIPVYLSGMARGLLGRDNKLQMRHKRKEAIKGSD
                     LIILAGVPNDFRLDYGNHIGGRRFISVNRSKTDLFKNKRPTLPILGDPMDFLIALSDY
                     FQAECPEWFGVLQSLNDNREQSIQEQAAAPVTTGMNPLALFRQLENQLDDDTILVADG
                     GDFVATASYILKPRTPLSWLDPGVYGTLGVGAGFALGAQLVFPEKQVWIIFGDGSAGY
                     SLMEYDTYKRHNLPIVSIIGNDACWSQIARDQVEFLKSDCAMNLAYSDYHIIPRAFEG
                     DGIRVDSLDKWQDAIQVAKDSRRKNIPFILNAIIGKTDFRKGSISV"
     gene            1512..2069
                     /locus_tag="PLG24_11830"
     CDS             1512..2069
                     /locus_tag="PLG24_11830"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HMZ25629.1"
                     /translation="MNKINSVFPTISETMLMVIDVQERLMPVIHDSHRVTHNINTLIR
                     GAAILGVDTLITEQYPKGLGNTIEEIDRSSNPPIFEKDCFSCLLQPNVSDFLFTKQKK
                     HLVLCGVESHICVLKTALDAESRGYTVHLVADAVSSRTPENKDLAIGRMRQSGIYIVS
                     VEMILFMLMERAGTDQFKAISKLIK"
     gene            2076..>2807
                     /locus_tag="PLG24_11835"
     CDS             2076..>2807
                     /locus_tag="PLG24_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HMZ25630.1"
                     /translation="MEIKRLFDFLTHQLERNPDLHFLSVKKTLFDDNKQWKHFSYRDL
                     QDNADKVSRLLLDAGIEKGDKIAIISENRPEWNFVDMGSQQIGVVNVPMYPTISEEDY
                     EFIFRDSGIKFAFVGNDTIYQKVLPLMAKLDGLKGIVTFDKLNGTRWLWDELEQIENP
                     GRQYIDAIKSTIVELDIATIIYTSGTTGNPKGVMLTHQNIVSNLTGVAKVLPFRDGEK
                     SLSFLPLCHSFERTVFYAYLMMNIHI"
BASE COUNT          788 a          574 c          677 g          768 t
ORIGIN      
        1 ctgctgtcaa gaatgcttca cttgcccagt caccgctcat tttactgggc ggtgctgcag
       61 caacgattct aaaggggcga ggcagccttc aggatataga tcaactatcc atcatgaaaa
      121 cggtgtgcaa atggtcagcc agtatttcca gggttaaaga tattgtgccg accctcgaaa
      181 aagcttttca aatagcacag gaaggcgttc ccggtcctgt tttcgtagag tgcccgattg
      241 atgtattgta cggtgaagaa ctggttcggg attggtatgg tgcaaagagt aaccaggagc
      301 cggctaaaaa ttttagggaa aaagccttgc gttggtacgt caacttccat gccaacaggc
      361 tttttagagg aaaagattcg gtcgactttg acaagccggt tgttgctccc atttttccta
      421 ttcatggtac aatggatgtg agtaaggcgg ctgagatgtt gagaaaagca aaaaaaccac
      481 tccttatcat tggctcagga gcgatgatgc aggctgccag ggcaggtgat ctggcacagg
      541 ctgttgaacg tctgggtatc ccggtctatc tgagcggtat ggcccgaggc ttgcttggca
      601 gagacaataa attgcaaatg cggcataaga ggaaggaagc cattaagggc tccgacctga
      661 ttattcttgc cggtgtcccg aatgatttca gactggacta tggaaatcac atcggaggac
      721 gcagattcat ctctgtcaac cgaagcaaga ccgacttgtt taagaataaa cggccgacat
      781 tgcctattct tggtgatcca atggactttt tgattgccct gtctgattat ttccaggcag
      841 agtgtcctga atggtttgga gtgttacaat cgctcaacga taatcgggag caatccattc
      901 aggagcaggc tgccgcaccc gtcactaccg gaatgaatcc attggccttg tttcgtcaac
      961 ttgaaaacca attggatgat gataccattc ttgtcgctga tggaggtgac tttgttgcta
     1021 cggcatccta tatacttaaa cctcgcacac cgttaagctg gctggatccg ggtgtctatg
     1081 gtaccttagg cgtgggagcc ggttttgcct tgggcgccca gttggttttt cccgaaaagc
     1141 aggtgtggat catctttgga gatggaagtg cgggatacag cttgatggaa tatgatactt
     1201 acaaaagaca taatttgccg attgtctcga ttattggcaa cgatgcctgc tggagccaga
     1261 tagccaggga tcaggtggaa ttcctcaaga gtgactgcgc tatgaatctg gcatatagcg
     1321 attatcacat catcccccgt gcatttgaag gcgatggtat tcgggtcgat tcattggaca
     1381 aatggcagga cgctattcag gtagcgaagg atagtaggcg aaaaaacata ccatttattt
     1441 taaatgcaat catcggaaaa acagactttc gtaagggctc aatcagcgtg tgatttttta
     1501 aaaattaaat aatgaacaag atcaattcag tattcccaac tatcagtgaa accatgttga
     1561 tggtgattga tgttcaggag cgactaatgc ctgtcattca cgattctcat cgtgtcaccc
     1621 acaatattaa caccctgatt agaggggcag caattctcgg tgtagatacc ttgatcacgg
     1681 aacaataccc gaaaggcctc ggcaatacca tagaagaaat tgatagatcg tcgaacccgc
     1741 ccatcttcga aaaagattgc tttagttgtt tgttgcagcc caatgtaagc gattttttgt
     1801 tcacaaagca gaaaaagcat ctcgtccttt gtggtgtcga aagccatata tgcgtgctaa
     1861 aaaccgctct tgatgcggaa tcccggggat ataccgtgca tttggttgcc gatgctgttt
     1921 cttcgcgtac accggaaaat aaggatctgg caataggacg gatgcgccag tcaggcatat
     1981 acatcgtttc cgttgaaatg atccttttta tgcttatgga aagagcaggt actgatcaat
     2041 tcaaagctat tagcaaactc atcaaataat aaatcatgga gataaaacga ttatttgact
     2101 ttcttactca tcaacttgaa aggaatcctg atttacattt tctttctgta aagaagacac
     2161 tctttgatga taataagcaa tggaaacatt tttcctatag agatttgcag gacaatgcag
     2221 ataaggttag ccggttgttg ctcgatgcag gaattgagaa gggcgataag attgccatca
     2281 tcagtgaaaa ccgaccggaa tggaattttg tggatatggg atctcaacag ataggtgtcg
     2341 tcaatgtgcc tatgtaccct acaatttccg aagaagatta cgagtttatc ttcagggatt
     2401 ctggcataaa atttgcattt gttggaaatg acaccattta tcagaaggta ttacctttaa
     2461 tggccaagtt ggatgggttg aagggcattg tgacttttga taaattaaat ggcacccgat
     2521 ggctttggga tgaattggag caaatagaga atcccggtag gcaatatatt gacgccatca
     2581 aatcaactat agtcgagctg gacatagcaa ctatcatata cacctcaggc accaccggca
     2641 atcccaaggg agttatgctc acgcatcaaa atatcgtaag caaccttact ggcgttgcca
     2701 aggtattgcc attcagggac ggtgaaaaat ctctcagctt cctgcccttg tgtcacagtt
     2761 ttgagcgtac ggtattttac gcttacctga tgatgaatat ccatatt
//
LOCUS       DAOQQN010000380         2804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_18971_length_2804_cov_5.066933, whole genome shotgun sequence.
ACCESSION   DAOQQN010000380 DAOQQN010000000
VERSION     DAOQQN010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18971_length_2804_cov_5.066933"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1307)
                     /locus_tag="PLG24_11840"
     CDS             complement(39..1307)
                     /locus_tag="PLG24_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HMZ25631.1"
                     /translation="MPPLAERMRPKVPGDYIGQRQIMGNDAPFRKLLEQKKIHSCIFW
                     GPPGVGKTSLALLIAEFTDRRIFALSAVNAGVKDVRETIDKASRVDLFHSGQAPILFI
                     DEIHRFNKSQQDALLEAVEKGTVILIGATTENPSFEVNQALLSRCQVYVLEELSADDL
                     KTMVDRAISRDSILSQQEIEVAEYEALIFFSGGDARKLYNILEILASMHVVGKLTINN
                     QLVEEAVKVKHIAYDKGGEFHYDLISAFIKSIRGSHPDAALYYMARMLLGGEDPIFIC
                     RRMIILAAEDIGLANPNALLIAQACMDAVHKIGMPECRIVMSECALYLASSPKSNSAY
                     LAINKAFDLVQKTGNLAVPLHLRNAPTGLMKELGYGKDYQYGHDFENNFVAQEYLPKE
                     IMSLKIYKPGRNPSEDRILSFLKERWKDKY"
     gene            complement(1313..>2804)
                     /locus_tag="PLG24_11845"
     CDS             complement(1313..>2804)
                     /locus_tag="PLG24_11845"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMZ25632.1"
                     /translation="DTVAIYYSASKDAVALQWLSPEQTYDKKYPFLYTQGQPNLTRSW
                     IPVQDSPGIRFTYDATLRVPAGLMALMSAVNPQAPVKDGVYYFHMDEPIPAYLLVMAV
                     GNLAFHAFDNRSGVYAEPGQLKAAAFEFSETPEMIRTAESLYGPYAWGRYDILVLPPA
                     FPFGGMENPRLTFATPTVIAGDRSLANLIAHELAHSWSGNLVTNATWNDFWLNEGFTT
                     YFERRIVEKVKNKDYADMNSIIGRNDLDIELADFGLDSQQSRLKQNLNGENPDDAMTD
                     IPYEKGYLFLVLLERTFGREKMDDFLNGYFEHFKFKTITTEEFVKYLYSKFDAVQLDK
                     IHTNDWIYKPGIPDNAPVFESKMFEAVEKEEPALIDGASRLAYKDWDFNQWLYFLRRI
                     KGKTGLNELNRLNESYHFLESQNTEILAIWLENNIVLKNTAINGKVNEFLGRVGRRKF
                     LEPLYKALLQSGQKDVALEIYRQHRSGYHSVSQHSLDKMLDYKPTK"
BASE COUNT          815 a          631 c          546 g          812 t
ORIGIN      
        1 cgtacaaaag cacgatccca gatattccag gaaatgattt aatatttatc tttccatcgt
       61 tcttttaaaa aagaaaggat tctatcttca gaaggatttc tacccggttt ataaatcttt
      121 aaactcataa tctctttagg aagatattcc tgagctacga aattattttc aaaatcatgg
      181 ccatactggt agtcttttcc atagccaagt tccttcatca atcctgtagg cgcattccgc
      241 aaatggagtg ggacagcaag attacccgtt ttttgtacca gatcaaaagc cttgttgatt
      301 gccaggtagg ccgaattgct tttaggtgag gaagccaggt aaagagcgca ttcggacatc
      361 acaatcctgc attccggcat accaatctta tggacggcat ccatgcatgc ctgggctatc
      421 agtagagcat taggattggc caggcctata tcctccgcag caaggatgat cattcggcgg
      481 caaataaaga tcgggtcttc tccacctaaa agcatcctcg ccatataata taaggctgcg
      541 tcaggatgag aaccacggat cgacttgata aatgctgaaa tcagatcata atgaaattca
      601 ccgcctttgt catatgcaat atgcttaact ttaaccgctt cttcaaccaa ttgattgttg
      661 attgtgagct ttcccacaac atgcatcgaa gctagtattt ccagtatatt atataatttt
      721 cttgcatcgc ctcctgaaaa gaaaatcaat gcctcatatt ctgcaacttc aatctcctgt
      781 tgagacaata tggaatcccg tgaaatagcc cggtcaacca ttgttttcaa gtcatcggca
      841 gagagttcct ccaatacata tacctgacaa cgtgacaata atgcctgatt gacttcaaag
      901 gatggattct cggtagtggc gccaataagg ataactgttc ctttttcaac agcctccaat
      961 agagcatcct gctgtgattt attaaaacgg tgtatctcgt cgataaataa tatcggtgcc
     1021 tgaccggagt gaaaaagatc gacccttgat gccttgtcga tggtttcccg cacgtccttg
     1081 acgccggcat tgacagcact taaagcaaaa attcgtcgat cggtaaattc agcaatcaaa
     1141 agtgcgagag aggtcttgcc cacaccgggt ggaccccaga aaatacaaga atgaattttt
     1201 ttctgctcca ggagtttccg gaatggagca tcatttccca tgatttgcct ctgaccgata
     1261 taatctcccg gcaccttggg tctcatcctt tcagctaatg gtggcataca atttacttgg
     1321 tcggtttgta atcaagcatt ttgtcgagtg agtgttgaga tactgaatga tatcctgaac
     1381 ggtgttgcct atagatttca agggccacat ctttttgccc cgattgtaac aaggctttat
     1441 acagcggttc cagaaatttg cgtcgtccca ctctaccaag gaattcattt actttcccat
     1501 taatcgctgt attcttcaac acgatattat tctccaacca aatagcaaga atttcagtat
     1561 tttgggattc aagaaagtga taactttcat tcaaccgatt caactcattc aaaccagtct
     1621 ttcctttaat ccttctgaga aagtacaacc attggttgaa gtcccaatcc ttatatgcca
     1681 gtctggaggc accatcgata agcgccggct cttccttttc aacagcttca aacattttgc
     1741 tctcaaacac aggtgcatta tccggtattc cgggcttgta tatccaatca ttggtatgta
     1801 ttttatccaa ttgaacagca tcaaatttgg agtaaaggta ttttacaaac tcttcggtag
     1861 taattgtctt gaacttgaaa tgttcaaaat atccattcaa gaaatcgtcc atcttttctc
     1921 taccaaaggt tctctcaagt aaaacaagga acaaatatcc tttttcataa ggaatatcgg
     1981 tcatggcatc atcgggattt tcaccattca ggttttgctt aagtcggctt tgttgactat
     2041 ccaatccaaa atctgccaat tcaatatcaa gatcattgcg acctataatt gaattcatat
     2101 cggcataatc cttattttta accttttcaa caatacgtct ttcaaaataa gtcgtaaacc
     2161 cttcattaag ccaaaaatca ttccaggtgg catttgtcac aaggttacca gaccaggagt
     2221 gagccagctc atgagcgatt aggttggcta atgaacgatc tcctgcgatg actgtaggag
     2281 tagcaaaggt caacctcggg ttctccatac ctccgaaagg aaaggcaggt ggtagaacca
     2341 ggatatcata ccgtccccag gcataaggcc catagagtga ttccgctgtt ctaatcattt
     2401 caggggtctc agaaaattca aaagccgcag ctttcaattg cccgggttct gcatacacgc
     2461 cacttctatt atcgaaggca tgaaatgcaa ggttacctac agccataacc aaaagatacg
     2521 cagggattgg ttcgtccata tggaagtaat atacgccatc cttcaccgga gcttgaggat
     2581 tgactgcaga catcaatgcc atcaaacctg caggtacacg gagtgttgca tcataggtaa
     2641 accggatacc cggagaatcc tgaacaggaa tccatgatct ggtaagattg ggttggcctt
     2701 gggtataaag aaaaggatat tttttgtcat aagtttgttc cggagatagc cactgtaggg
     2761 caacggcatc cttacttgca gaataataaa ttgcgacggt atct
//
LOCUS       DAOQQN010000381         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_19653_length_2745_cov_5.914498, whole genome shotgun sequence.
ACCESSION   DAOQQN010000381 DAOQQN010000000
VERSION     DAOQQN010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19653_length_2745_cov_5.914498"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="PLG24_11850"
     CDS             complement(<1..1093)
                     /locus_tag="PLG24_11850"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ25633.1"
                     /translation="MIANLPETELKRVVIIGAGFGGLKLARKMIGSGYQVVLIDRENY
                     HQFQPLLYQVATAGLEPSSIAFPLRKVFQKETNFHIRIDEVRFINLDEKFILLKNIGR
                     VNFDKLILAYGADTNFFGNENISKYSIPMKSVPEALALRNRILSDYERALSITDYDLR
                     QELMDIVIVGGGPTGVEIAGALADMRQFILPKDYNELDKQEVDIYLVDSGNKLLAAFD
                     EKSSLHAERDLESLGIHILKENRVIDYDGLNVAMSDGKIIRSRKLIWAAGITVKKIDG
                     LPETIYSRSGRINVDGYNQVIGLEDVYAIGDCAIMVTDDKPNGDPQVAQVAMQQAVTL
                     AKNLKAMIKGTTLKTFHYHDKGIMATIGRK"
     gene            1320..2357
                     /gene="holA"
                     /locus_tag="PLG24_11855"
     CDS             1320..2357
                     /gene="holA"
                     /locus_tag="PLG24_11855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HMZ25634.1"
                     /translation="MAEYSFEDIEKQINNRKLHPVYILQGEETYFTDLLTRKFQDELL
                     EEDEKAFNQEVYFGKETTVSTIIDSLSQFPMGSDYKLVLIRNAQELKDIDKLTSYVND
                     PFESSVLVLSFTGKSMDGRKKLPSIVKSKYGFFEAKTIYENQLPAWIKSEVKTKGKEI
                     SDQNATLIADYLGKDLQKISNELDKTVIAIGDAKEINQEHIENYIGINREFNVFELIN
                     SIFNKNPYKARLIVEYLTDNLNKQPLTLILNSLFNSFLKLYLLKVNNPKSDMDITAII
                     GTRSISAIKEYRTAMANFSLPDLDYAMYLFKIHDQLSKGIDSGNADHGELLKELVFKL
                     LHVKEVQVCPK"
BASE COUNT          890 a          570 c          464 g          821 t
ORIGIN      
        1 ttttccgtcc gatagttgcc atgatacctt tgtcatgata atggaatgtt tttaaagttg
       61 tccctttgat catagcttta agatttttgg ccagggttac tgcctgttgc atggcaacct
      121 gagctacctg cggatcccca ttgggcttat catctgtcac cataatagca cagtcaccaa
      181 tagcgtagac atcttccaaa ccaattacct ggttatatcc atccacattg atcctgccac
      241 tacggctata aatagtttcc ggcaatccat cgattttctt tactgtaatg cctgctgccc
      301 atattaattt tctggaacga attatctttc catcagacat ggcaacattc aatccatcat
      361 aatcgatcac acgattttcc ttaagtatgt gaattcccag gctttcaaga tccctctctg
      421 catgaaggct ggatttttca tcaaaagcgg ccagcaattt atttcctgaa tcaacgaggt
      481 aaatatccac ttcctgctta tccaattcat tgtaatcctt tggaaggata aattgacgca
      541 tatcggccaa tgcgcctgca atctccacgc cggtcgggcc gccaccgact attacaatat
      601 ccatcaattc ctgcctgagg tcatagtcgg tgatggaaag tgctctctcg tagtcactca
      661 ggattctatt acgcaaagca agtgcctcag gtactgattt catcggtatt gaatatttac
      721 taatattctc atttccaaag aaattagtat ctgcaccata ggcaagaatc agtttgtcaa
      781 aattaaccct accgatattt ttcagtaaaa tgaatttctc atccagattt ataaatctaa
      841 cttcatctat tctgatatgg aagttcgttt ctttctgaaa gactttccgc aatggaaaag
      901 caatgctact gggctccaga ccagcagtag ccacctggta caacaggggt tgaaactgat
      961 ggtaattctc cctgtctatc agcacaacct gataaccact tccgatcatc ttcctagcga
     1021 gctttaagcc accaaatccg gcgccgataa tcactactct ctttagttcg gtttctggaa
     1081 gatttgcaat catgcctaaa atttattttt aaaatgtcaa agctaaaaaa agattggtgt
     1141 atgagactct caagtattga aaccacctaa actattactt taagttaaaa tatctttatt
     1201 gttgatttta ttatatttaa ataatattgt atatctaaca ataaatatag ttaaaagaaa
     1261 aataacaaaa ccttcattcg cgacaatatc attaccttcg tccaataatt gcgaattgga
     1321 tggctgaata ttcctttgaa gatattgaaa agcagattaa caaccggaaa ctgcaccctg
     1381 tgtatatcct tcagggtgaa gaaacctatt ttactgattt gcttacgcgc aaattccagg
     1441 atgaattatt ggaggaagac gagaaagcat tcaatcaaga ggtatatttc ggaaaagaaa
     1501 ctacggtttc aaccatcatt gacagtctca gtcaatttcc aatgggatct gactacaagc
     1561 tggttctcat ccgtaatgcc caggaactta aagatattga caaattaaca tcctatgtaa
     1621 acgatccttt tgaatcgtct gtcctggtac tttcattcac agggaagtca atggatggaa
     1681 gaaaaaaatt accttcaatc gtcaaatcaa aatacggatt ctttgaagca aaaacaattt
     1741 atgaaaatca actaccagca tggataaaat cggaagtaaa aaccaagggc aaggaaatat
     1801 cggaccaaaa cgccacatta atcgctgact atcttggtaa ggatctccag aaaatatcga
     1861 atgagttgga taagaccgtc atcgctatcg gtgatgccaa agaaattaac caggaacaca
     1921 ttgagaacta tatcggcata aatcgtgaat tcaatgtctt cgagcttatc aattccattt
     1981 tcaataaaaa cccatacaaa gccagactta tagtggaata tctcactgac aacctgaaca
     2041 agcaaccatt gacattgatc cttaattcac ttttcaacag cttcctgaaa ctctatttat
     2101 taaaagtaaa caatcccaag tcagatatgg acattactgc aataatagga accagaagta
     2161 tatctgccat aaaggaatac cggacagcaa tggcgaattt cagccttcca gatcttgatt
     2221 atgctatgta tctgtttaag attcatgacc aactttctaa aggaatagat tcggggaatg
     2281 ctgatcatgg agaacttctt aaggagctgg tttttaaatt attacatgtc aaggaggtac
     2341 aagtatgtcc aaaataatgg ctgatttcct tggccggctt actcttgtta tctgaagtat
     2401 agttaaatta aacgataacg cgatttttgt agattttgtc atcaacccga gaaaaaattc
     2461 taagaaagga caaaatttta agtaggtaga agattaatag tctcaaatta agcctctcca
     2521 atagtttctg acaaacaaga aatattttga aacccaaatt aacatattct ttccgggagg
     2581 caatatgaaa agctcagttc tgcaagaagt ctcgattatt aatcatctac ctacttcggc
     2641 acactttagt ggtctcaccc aatgttgttc gtttggcaaa aattcaagta agtagatgat
     2701 taatagcccg aatctacgtc acaacgctac ttcccaattc gtatg
//
LOCUS       DAOQQN010000382         2706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_20138_length_2706_cov_5.820822, whole genome shotgun sequence.
ACCESSION   DAOQQN010000382 DAOQQN010000000
VERSION     DAOQQN010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20138_length_2706_cov_5.820822"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLG24_11860"
     CDS             complement(<1..409)
                     /locus_tag="PLG24_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25635.1"
                     /translation="MQRPDHRLSFKPFFIRGMVVVFLSFASWCNYALYGQQRITHSVD
                     FSSYQSKSVEGLNTHGILLFPQSEITIFNKVALVSTSLPIAKGKRAKIIDFKPYFNTQ
                     LPLDLPYREGLRNVEPILETQKGRVENRIEITFL"
     gene            818..1819
                     /locus_tag="PLG24_11865"
     CDS             818..1819
                     /locus_tag="PLG24_11865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMZ25636.1"
                     /translation="MRLNILILGFILFSFVANSQDPVFSQFYAHPLELNPALAGNSGG
                     TRFGINYRNQFSGLPSNYTTYAISADQYFPGIRSGVGISVMADEAGQGIYRTYNGELS
                     YTYGVEFRNGTKVKMGLQAGFISVTLDYDKFIFMDQIDPIHGPISPGGIPYPTAEAPP
                     DFRNRTLFDMGAGGLLSNDKFYVGIGLKHLNRPDINFYAAENNTKKGLSMRISAMAGF
                     RIPIGDIRYGHRSEMAIIPNLLIVKQANLGQINAGALLEIKALQFGLYYRNGFLNPDA
                     IILSVGYNSGQFKVAYSYDATISKLGINSGGAHELSLSYVLFQDDGGKYHDCTKIFR"
     gene            2037..>2706
                     /locus_tag="PLG24_11870"
     CDS             2037..>2706
                     /locus_tag="PLG24_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HMZ25637.1"
                     /translation="MNQAFKIFILFGCVLFLFASCKKKEERSSTTGWVYNDQKWGGFT
                     KSNAIDQPTGPNLVLVEGGTFTMGMTQEDVTYEWNSVPRRVTVSSFYMDETEVANIDY
                     REYVFWTKRVFGDNYPQVWRNTLPDTMCWREELAFNEPYVETYFRFPSYDDYPVVGVS
                     WIQANEYCKWRSDRVNEMMLIEKGILNPNPEQKDSDNFNTQAYLYGQYEGSVKKNLKD
                     VSTGG"
BASE COUNT          870 a          496 c          570 g          770 t
ORIGIN      
        1 gtaaaaaagt aatttcaata cgattttcaa ccctgccttt ttgtgtttca aggataggtt
       61 caacgttgcg gaggccttcc cggtagggca agtcaagagg caattgggtg ttgaaatatg
      121 gcttgaagtc gataattttg gctcgcttcc cttttgcaat tggtaacgaa gtggatacta
      181 atgccacctt gttgaaaatg gttatttcac tctgaggaaa tagcaggatt ccatgtgtat
      241 tcaatccctc aactgacttg gactgatagg atgagaaatc aacggaatgt gtaatcctct
      301 gttgaccata aagtgcataa ttgcaccaag aggcaaagga taagaacaca accaccattc
      361 ctcgtataaa aaaaggcttg aaagaaaggc gatgatccgg acgctgcata tgaaggtcgt
      421 tgtatttcaa caaaattaat aaaatggtag taggatatat ttaaaataaa atcagaatac
      481 ttaaaacgag tatggaatca aattattatc aaatcatcaa caaagctatt aatttaacac
      541 atgctcatta tccgtaatga attaaaattt gatgttatcc aaaaactata ccaaattttg
      601 tttttaaatt aaaaaaataa ataatatatt acaagttgcc cggaaatcct ggttagtttg
      661 ggaggaatac ttttataagc aagtaagtaa actgattaat tcgcctttgg gaattaatat
      721 tttttcaaat gcacaagctg taatccatct ctgcctctaa aaagaaaatc aagtaaatat
      781 aaaaaacgtt acatttgaca tttcaaacgg gtaagtaatg agattgaata ttctgatact
      841 gggattcata ctgttttcct ttgtggctaa tagccaggat ccggtttttt cccaatttta
      901 tgcccacccc ctcgagttga atcctgcact ggcaggtaac agcggaggta ccagatttgg
      961 gatcaattac cggaatcaat tctccggact tccttccaac tacaccacct atgccataag
     1021 tgcagatcaa tattttccgg gtattcgcag tggtgtgggt atttcagtca tggcggatga
     1081 ggcaggccag ggaatttaca gaacatataa tggcgagctg tcctatacgt atggcgtgga
     1141 gttcagaaac ggaactaaag taaagatggg acttcaggcc ggatttataa gcgtcacact
     1201 tgattatgac aagtttattt tcatggatca aatagatcca attcacgggc cgataagtcc
     1261 cggtggtatc ccttacccga cagcagaagc acctcctgac ttccgcaaca ggacgctttt
     1321 cgatatggga gccggaggtt tactgagcaa tgacaaattc tatgtaggta ttggcctgaa
     1381 acaccttaat agaccggata tcaattttta tgctgcagag aacaatacca aaaaaggact
     1441 atctatgagg atttctgcca tggccggatt caggattccg ataggtgaca taagatatgg
     1501 ccatcgatcg gaaatggcaa ttataccgaa ccttctcatc gtcaagcaag caaatcttgg
     1561 acagataaat gccggtgccc tgttggaaat caaagcatta caattcggat tatattatag
     1621 aaacggcttt ctgaatccgg atgccatcat ccttagtgtc ggttataatt caggacaatt
     1681 caaagtggca tatagctatg atgccaccat cagcaaactg ggcatcaatt ccggaggagc
     1741 ccatgagttg agcctcagtt acgtcttgtt tcaagatgac ggaggtaaat atcacgactg
     1801 cacgaagatt tttagataaa tatgcatttt tacaaaacat aatatcataa aaaggtaaaa
     1861 aatacgttta aaaaaagtgt aggtaaaatt ttgtacagaa ttaattagtt gtatctttga
     1921 ccttccaaaa ttcaaacaag tagatttaac agagtttttg gattcatatt gagtttactt
     1981 acgctaaatc aataagttgc aaaatttctg gaatttttta aaactgaaaa attataatga
     2041 atcaggcatt caaaattttt attctttttg gctgtgttct cttccttttt gcatcatgta
     2101 agaagaaaga agagcgctca agcacgaccg gctgggtata taatgaccag aaatggggtg
     2161 gctttaccaa atccaatgcc atcgatcagc caacagggcc aaacctggtt ttggttgaag
     2221 gtggtacttt tacgatgggt atgacccagg aagacgtgac ctacgaatgg aacagcgtgc
     2281 cccgcagggt gaccgtgtct tcattttata tggatgagac cgaagtggcc aacattgatt
     2341 acagagaata tgttttctgg accaagagag tttttggcga caattatcca caggtttgga
     2401 gaaatacact tccggacacc atgtgctggc gagaagaatt ggcattcaac gagccttatg
     2461 tggaaactta tttcaggttt ccctcctatg atgattatcc ggtagtgggc gtttcctgga
     2521 ttcaggctaa tgaatactgt aaatggagat ctgacagagt caatgaaatg atgctcatcg
     2581 agaaaggcat tctcaatccc aatcctgagc agaaggacag cgacaacttt aatacacagg
     2641 cttatcttta cgggcagtat gaaggcagtg taaagaaaaa ccttaaagat gtttcgaccg
     2701 gaggtg
//
LOCUS       DAOQQN010000383         2701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_20206_length_2701_cov_6.092593, whole genome shotgun sequence.
ACCESSION   DAOQQN010000383 DAOQQN010000000
VERSION     DAOQQN010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20206_length_2701_cov_6.092593"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PLG24_11875"
     CDS             complement(<1..600)
                     /locus_tag="PLG24_11875"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HMZ25638.1"
                     /translation="MRIISGLFLFAACFLSGYVLNAQIKTPAASPGSTVKANVGLGEA
                     TVEYSRPSKKGRIIFGDLVPFGKIWRTGANGATKITFSEKATVGSSELAKGTYAIYTI
                     PNPNEWTVIFYKDLTKGGGLTDANIKSEDVAAKIVVQTAMINPGVETFTIDIADVTNK
                     GASIAIMWDKTMVKVPFTYNTDAAVMANIKEKMAGPSGND"
     gene            complement(773..1456)
                     /gene="rpiA"
                     /locus_tag="PLG24_11880"
     CDS             complement(773..1456)
                     /gene="rpiA"
                     /locus_tag="PLG24_11880"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HMZ25639.1"
                     /translation="MLTQDQIKEKIGIHAADYIKPGMKVGLGTGSTVFYLLKELGRRK
                     AQGLHFTAVCTSVQTQRVLIEEGIEFLTLDEVDRLQIAIDGADEIDPAGNMIKGGGGA
                     LLREKIVEYNADELIIIIDEKKRVNNLGAFPLPVEVVTFGWKQVRQKLANDYGIIVLR
                     REHDSKPFITDQGNYILDCHFSLITDPQQLNTDIQLIPGVVETGLFVGMADKAITGYA
                     DGSIEFIEF"
     gene            1771..>2701
                     /locus_tag="PLG24_11885"
     CDS             1771..>2701
                     /locus_tag="PLG24_11885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HMZ25640.1"
                     /translation="MISISNLYIQYGDRILFDSISTTINIGEKIGLTGRNGAGKSTLL
                     KILAGEITQFDGVVDRPRNETVAYLHQDIEISGEHSVIEEAMKAFEELNEIERQIQIA
                     EHDLTIRDDYESDSYAKLIDNLTHLTEQFEYRGGNQKEANAEKILKGLGFKSHEFSKK
                     VKELSGGWQMRVELAKMLLTKPDYLLLDEPTNHLDIESIIWLESFLSDYKGAVVMISH
                     DKIFLDKITRRTIEIELGRIYDYQANYSDYLLQRVERRALMMAAYENQQKLIAQKEKT
                     IARFMAKATKTSMAQSMQKQLDKIERIELEDEDT"
BASE COUNT          806 a          521 c          569 g          805 t
ORIGIN      
        1 gtcattaccg gaaggaccgg ccatcttctc cttaatgttg gccatgactg ctgcatctgt
       61 gttgtaggta aacggaactt tcaccatagt cttatcccac atgatggcga tactggcacc
      121 tttgttggta acatcggcaa tatcaattgt aaatgtctct acaccggggt ttatcatggc
      181 tgtttgcacc acgattttag cggccacatc ctctgatttg atgttggcat ctgtaagtcc
      241 acctcctttt gtaagatctt tatagaagat tacagtccat tcattcggat tgggaatcgt
      301 gtagatagcg tatgttcctt ttgccaattc actggagcca actgttgcct tttcagaaaa
      361 ggtgattttt gtcgcaccat tggcaccggt acgccatatt ttaccgaatg gtacaaggtc
      421 gccaaaaata atcctgcctt tcttggaagg tcttgaatat tctacggttg cttcacccaa
      481 acctacatta gctttaacgg tagagcccgg agaagctgca ggggtcttga tttgtgcatt
      541 tagaacatag cctgaaagaa agcaggctgc aaaaagaaaa agtcctgaaa ttattctcat
      601 tattatatgg ttttgttgat aaaaaacaat tcaaaggttt attcgaaggt taacacagat
      661 tgaatgaaaa ggttcaatcg tttgagagaa aagtatagaa cttattgata aagaaaatat
      721 tagggtcaaa taagtccaaa gataaataaa tggagttcct cctccgatcc cgttaaaatt
      781 caatgaattc aatggatcca tctgcatatc ctgtaattgc tttgtctgcc atacccacaa
      841 aaagacccgt ttcgacaact ccaggtatca attggatgtc ggtattgagt tgttgtggat
      901 cagtaatcaa gctgaaatga caatccagga tataattccc ctggtcagtg atgaagggct
      961 tgctatcatg ctcacgtcta agtacaataa taccatagtc gtttgctaat ttttgacgaa
     1021 cttgcttcca gccaaaagtg accacttcga ccggtagtgg aaatgcaccg aggttgttaa
     1081 ctcgtttttt ttcatcaatg attatgatca gttcatcagc attgtattct acgattttct
     1141 cccgaagtaa tgcgcctcca cctcccttga tcatattgcc ggcaggatca atttcatcag
     1201 caccatcaat tgcaatttgt aatctatcca cttcatccag agtgaggaat tcgattcctt
     1261 cttcaatgag tacacgctgc gtttgtacgg aagtgcatac ggcagtgaaa tggaggcctt
     1321 gcgcctttct tcgccccagt tccttcaata ggtaaaaaac agtcgagcct gtacccaatc
     1381 cgaccttcat acccggtttg atataatcag cggcgtgtat tcctattttt tccttgattt
     1441 gatcttgtgt aagcatggtg taaatgtaag ggtaattttg gtaaagcaga atgatttaag
     1501 ggttagaata ttgacgaaaa taggttggta aatgaaaggc tgcaaaaaga agtatatttt
     1561 taagttttgc tccggagtgt ttattttaaa atttttttcc atttccaaca gggctgcata
     1621 ccatttgtat agtgtagtgt atttggtctg attctgaaca aattatatca tcgtcggaca
     1681 tacgtttgtc aatttgtagt tatgcattga tgattaggaa agttttgctt tactttgcag
     1741 ccttaattta gtgaacattc gatttctata atgatttcaa tcagcaatct ttatattcaa
     1801 tacggtgaca gaatcctttt tgattctata tcgactacga tcaatatcgg agagaaaata
     1861 ggccttacag gaagaaatgg tgcaggcaaa tcaacgctgc tcaaaatatt ggcgggtgaa
     1921 attacccagt tcgacggggt ggtagaccgt ccccgtaacg agactgttgc gtatctgcac
     1981 caggatattg agatcagcgg cgaacactct gtaatagaag aagctatgaa ggcctttgag
     2041 gagttgaatg agattgaacg acagatacaa attgccgagc acgatctgac tatacgcgac
     2101 gattatgagt cagactccta tgctaaactc atcgataatc taacacatct tacagaacaa
     2161 tttgaataca ggggaggaaa tcaaaaggaa gctaatgcag aaaaaatatt gaaagggctt
     2221 ggttttaaaa gtcatgaatt ttccaagaaa gtcaaagaat tgtccggtgg atggcagatg
     2281 agggtcgagc tggccaagat gttgcttact aagccggatt atctgttact ggacgaacct
     2341 accaaccacc tggatattga atcaattatt tggctcgaat ctttcctgtc tgactataag
     2401 ggtgccgtag tgatgatctc acacgataag atctttttgg ataaaattac caggcgtacc
     2461 attgaaattg aattgggtcg aatctatgat tatcaggcca attattctga ttatctgtta
     2521 caaagggtgg agcgcagggc tctcatgatg gctgcctatg aaaatcagca aaagctgatt
     2581 gcccagaaag aaaagaccat tgcccggttt atggccaaag ccactaaaac ttccatggca
     2641 caatccatgc aaaaacaact ggataaaata gaacggatag aactggaaga tgaagataca
     2701 t
//
LOCUS       DAOQQN010000384         2681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_20466_length_2681_cov_4.903275, whole genome shotgun sequence.
ACCESSION   DAOQQN010000384 DAOQQN010000000
VERSION     DAOQQN010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20466_length_2681_cov_4.903275"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PLG24_11890"
     CDS             complement(<1..535)
                     /locus_tag="PLG24_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HMZ25641.1"
                     /translation="MKNITVFCGANSGANPEFTLIARKVGSLLAGRGIGVVYGGAKIG
                     IMGAVADGALARGGRVTGILPHFLSAREIAHDGLTELVVVDSMHERKLKMYAMSDGFL
                     VLPGGFGTMDELFETLTWAQLGLHQKPIGILNTGHYYDELIALVERMAAEMILKDIYR
                     SMMIVGDEIEELLSKMEN"
     gene            1295..2497
                     /locus_tag="PLG24_11895"
     CDS             1295..2497
                     /locus_tag="PLG24_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HMZ25642.1"
                     /translation="MSNPVYFEEHLPKTEMLEVKKKHANLSIGIIREQMEMENRVALV
                     PASVAYLVGNNHKIVVETGAGMRSNFSDHEYSEAGAEIVQSTKEAFKCDVILKVAPPT
                     HEELDNFRPGQILISPLQIPLINSDYINKLRQKRVIALAMEYMMDDDGSFPLVRIMSE
                     LAGISAILTAANLLTTTSGGTGVLLGGISGVPPAKVVILGGGVVAEYAVRTALGLGAS
                     VCVFDNNIYKLMRIQARVGQRLFTSSINPTYLGQALSDADVAIGAVHSKAGRTQLIVS
                     EEMVARMKPGSVIIDVSIDQGGCFETSEVTSLDRPTFEKHGVIHYCVPNIASNVPRTA
                     SVAISNMITPILAKAGSAGSIENVLFSHINLRNGVYTYKGCLTNKYLSDRFAIKYTDL
                     DLLMTSTF"
BASE COUNT          764 a          623 c          608 g          686 t
ORIGIN      
        1 aattttccat tttgcttaaa agttcctcga tttcatcacc gacaatcatc atcgagcgat
       61 agatatcctt gagaatcatt tcagctgcca tgcgttcgac cagcgcgatg agctcatcat
      121 agtagtggcc ggtattcagg atgccgattg gtttttgatg cagacccaac tgtgcccagg
      181 taagcgtctc gaacaattcg tccatggtgc caaagccgcc cggcaggacc aggaagccgt
      241 cagacatggc gtacatcttc aacttccttt cgtgcatcga gtcaacgacg accaattcgg
      301 taagaccatc atgtgcaatc tcacgagcac tgagaaaatg aggaaggatg ccggtgactc
      361 tgcctccccg ggcaagggca ccatcggcta cagcacccat gatacctatt ttggcgcccc
      421 catacactac gcctatgccc ctcccggcaa gcagggagcc gacctttcgg gctatcaggg
      481 tgaattcagg gttggcaccg ctgttggcac cacagaaaac ggttatgttt ttcaaatgca
      541 tattttgaaa gttattaagg gtattgaatc cttcggtcta tctatgtcat tggcagtcct
      601 caacatccga caaggccttg tgtgttatat cttttgtgta aatgaatatt cagaaggcaa
      661 tgaataaaac ccgattcaaa aataacaatc atcggtaaac agagataaat taattttcag
      721 atttaactga atgtcctaaa aggaaggtaa taccatcttc aagcattcag aatagaagga
      781 taacaaagcc aaaagagtgt gcagccgcgc cgatctgacg atggtctgac taagcccact
      841 tttattacat atcggaggcc gaaacaaccc cgggtaaaaa gaagtatttc cggcaaattg
      901 aatatcaaca gggagttttc tgaaaacact gttctatttg gttttcatcc gaaagcggcc
      961 caatcagtat catactaaaa gtacgatttc aattttattt ttaatcaaat ataaatcatc
     1021 ctgtcaccat tgaactatcc gtaaattcaa ggaatcatag aaaagggaag tttacatcct
     1081 ctcatctgtt ctgctgtgcc gattgaagga tgcggcctgc gaaagttcgt gcatttgaca
     1141 ctttaaccta tttctaccac aaggttgttt taagaaagct tttcaggaat gcttttgtgt
     1201 ttacatttca ttcattcgca atatgtaatt gacataaatt tgtatcttta accagccaaa
     1261 ggctaatatt ggaaacaaca attaatgagc aacagtgtca aatcctgttt attttgaaga
     1321 acatcttcct aagacagaaa tgctggaagt aaagaaaaag catgccaatc tgtcgatagg
     1381 aatcatccgg gaacagatgg aaatggaaaa tcgtgtcgct ctggttccgg catcggtggc
     1441 ttatttggtt ggtaataacc ataagatcgt cgttgaaaca ggtgcgggca tgcgttccaa
     1501 tttctccgat catgaatatt cggaggcagg tgcagagatt gtacaaagta caaaagaggc
     1561 attcaaatgc gatgtcatcc taaaagtcgc accgccaacc catgaagagt tggacaactt
     1621 tcgtccgggt cagattctga tatcaccatt gcaaataccg cttatcaaca gtgattacat
     1681 caacaaactg cgtcaaaagc gggtcattgc cctggccatg gaatatatga tggatgatga
     1741 cggttcgttc ccacttgtac ggatcatgag tgagctagcc ggcatcagcg ccatcctgac
     1801 cgctgccaat ctcctcacca cgacatccgg agggacaggc gtccttttgg ggggcatttc
     1861 cggtgtgcct cctgccaagg tagttatcct tggtggcggt gtcgtagcgg agtatgcggt
     1921 gcgcacggca ttgggactag gcgcttcggt ctgtgtattt gacaacaata tctacaaatt
     1981 gatgcgcata caagcccggg tagggcagcg tcttttcacc tcttccatca accccaccta
     2041 tcttggccag gccctttccg atgccgatgt ggctattggg gcagtacaca gcaaggcagg
     2101 tcggacacaa ttgatcgtgt cagaagagat ggtcgcccga atgaaaccgg gcagtgtgat
     2161 cattgatgtt agtattgatc agggcggctg ctttgaaaca tccgaagtga cgtcactcga
     2221 tcgtccgact tttgaaaaac atggcgttat acactattgt gtacccaaca tcgcctccaa
     2281 cgtaccccgc actgcatccg tagccatcag caatatgatc acgccgatcc ttgccaaggc
     2341 cggaagtgcg ggaagcatcg agaatgtact gtttagccat atcaatttgc gcaatggcgt
     2401 ctatacctat aagggctgtc tcaccaacaa atatctttcc gaccgatttg ccatcaagta
     2461 cacggacctg gacctgttga tgacctcgac cttctgatga ccaacgaatc ttgaggggga
     2521 cgaaaaggag ttttttggaa aaacagaagt aggtaggaaa ataatagacg gcagtcccgc
     2581 cacaacaaga cttgagggaa accgaattaa atcaactgca caaatacgcc tgaaatgatg
     2641 tcccgctttg aggaaaaggc tctaaaatca cgtgtacagc g
//
LOCUS       DAOQQN010000385         2643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_20935_length_2643_cov_4.907651, whole genome shotgun sequence.
ACCESSION   DAOQQN010000385 DAOQQN010000000
VERSION     DAOQQN010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20935_length_2643_cov_4.907651"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PLG24_11900"
     CDS             <1..352
                     /locus_tag="PLG24_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25643.1"
                     /translation="AGVALLLSLNPKDSILNLVGNAWAGFGSAFGPLIVLSLLSRKTT
                     WQGALAGMLTGGITVLLWVYLDHPYKDWYEMIPGFLSSLVCNLIVSQLTYKKNPVIEA
                     EFEQVEKMINDNQR"
     gene            490..822
                     /locus_tag="PLG24_11905"
     CDS             490..822
                     /locus_tag="PLG24_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25644.1"
                     /translation="MNWDQSEKKSREASSNQINESSEAASAFSRPAVPFQLKASEEFI
                     HENREKESTGSEYFPLNSSENGPKSFPAPPIQLKSEVVQRKADDEEGEAGMSHSSSGN
                     ENLPEEEL"
     gene            complement(1013..1615)
                     /locus_tag="PLG24_11910"
     CDS             complement(1013..1615)
                     /locus_tag="PLG24_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25645.1"
                     /translation="MDHVLIIERYLDTLGGRLLIGKMRSHYLQCTLSTESTQQIWREF
                     YNYQNNVFNISVELNNRSLMKIEGNRKYGVKRQGKVEHSLSQEELSSLHMSIQLVPEL
                     EFLNPGFGTQGIEVKESKNDYRIKLFLAPFGYEFCFDKISCLKKEVIIENINQEDSKL
                     VLKYSDWRVVDGLYYPFFQEMSGKHLLSYRSVDQLVLKWA"
     gene            1659..2618
                     /locus_tag="PLG24_11915"
     CDS             1659..2618
                     /locus_tag="PLG24_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25646.1"
                     /translation="MPEITYSSIEHLIQDVTTTGHQVQCSFYHLNGELISSNASIPHN
                     NSTMNRVTRVVKNQMINRARATASQSIFSLLGGGILGRAGSTIIRASVPANSSFSFEP
                     SQDDINDAVVKAFEKISTRYRYDENSGNWTPTDSPNSRSGKPTPKPNSPTKEVKNRPE
                     PHKEPGLNRLEKEVLARVLVEICDADGNLSGEEAEFLNKILPKELGSVEKIRNMDYIS
                     PLELKALSENAKKQIVKCAFILSYSDLQPHETETGIIQEYATQMGLSSQVLRQMEKEA
                     QKELVMTMIDENTTRAEVYEIGRQINLSDEESEMILIHKKESM"
BASE COUNT          865 a          541 c          501 g          736 t
ORIGIN      
        1 agccggagta gccctgttac tttccttaaa ccccaaggac agcatcctca acctggtagg
       61 aaatgcttgg gccggcttcg gttcagcctt cggacctctg attgtattgt ccttgttgag
      121 cagaaagacg acctggcaag gagcgttggc cggcatgtta acaggaggca taactgtttt
      181 actctgggta tatctcgacc atccttacaa ggattggtat gaaatgattc cgggtttcct
      241 gtcttcatta gtctgtaatc tcatcgtttc acaactgacg tacaagaaga atccggtaat
      301 tgaggcggag tttgagcagg tagagaaaat gattaatgac aaccaacgct agaaacaata
      361 ttatactgcc gtattcgggt gtatttattt atataattgt cttactttaa taaaatactc
      421 agaccaatta aaagaaagta aagcattgtt ttattaattt tacatgcaca ttaaataaaa
      481 tctccatcta tgaactggga tcaatctgag aaaaaatctc gggaggcttc ctccaatcaa
      541 atcaatgaat catcggaggc tgccagtgct ttcagtcggc cagctgttcc ctttcaatta
      601 aaagcctccg aagaatttat ccatgaaaat cgagagaagg aatctacagg ttctgaatat
      661 tttccattga attcatccga aaatggacca aaatcatttc ccgccccacc cattcagtta
      721 aagagtgaag tggtacaacg aaaggctgat gatgaagaag gcgaggcggg catgagccat
      781 agttcatccg gaaatgaaaa tttgcctgag gaagaattat aattatcgtt gcacaaaagg
      841 tactaataag tgagccttta ttcgcttggt aatttatact gattaatatc ggatcaatat
      901 aattctggat tcctcttcta tcgtgcaata cattgacatt cccctaattc tcccatgata
      961 acatttcata caaatgtgac aacttataag gtccaataga atccatgagc aactatgccc
     1021 atttcaaaac cagctgatca acggatcggt aggataaaag gtgtttgccg gacatttcct
     1081 ggaaaaaagg atagtacagg ccgtctacca ctctccagtc agaatatttt aagaccaatt
     1141 tcgaatcttc ctgattgata ttttcaataa ttacttcttt tttcaagcaa gaaatcttat
     1201 caaagcaaaa ttcataccca aaaggagcaa gaaacaactt tatccgataa tcgttcttac
     1261 tttctttaac ctctattccc tgggtcccga atccgggatt gagaaattca agttcgggga
     1321 caagctggat ggacatatgg agtgaactca attcttcctg cgacagagaa tgctcaacct
     1381 tcccctgcct tttaacgcca tattttctat tgccttctat tttcattaaa gaacggttat
     1441 ttagttccac tgaaatattg aagacattat tttgataatt gtaaaattct cgccatattt
     1501 gttgggtact ttcagtgctg agcgtacact ggagataatg acttcgcatt ttaccaatca
     1561 atagtcgccc gccgagagta tcaaggtatc tttcgattat caaaacatgg tccataagat
     1621 tctattcaca tttatttaca aaattaacga ttattaaaat gcctgaaata acttattcat
     1681 ccatcgaaca cttgatacag gatgtgacaa caacaggaca tcaagtgcaa tgttcatttt
     1741 accacctgaa tggtgaactg atcagcagta atgcttcaat tcctcacaac aactctacga
     1801 tgaatcgcgt cacgcgtgtc gtaaagaatc aaatgattaa cagggctaga gctactgcat
     1861 cacaatctat tttcagtctt ttaggaggtg gaattttagg cagggcagga tccacgatta
     1921 tcagagcttc ggttccggcc aacagttctt tttcttttga accaagccag gatgatataa
     1981 atgacgctgt agtaaaagcc tttgagaaaa tttcaaccag gtatagatat gatgaaaatt
     2041 ccggcaattg gactccaact gattctccaa acagtagatc cggaaaaccc actccaaagc
     2101 ccaacagccc gaccaaagaa gtgaagaatc gcccggagcc acacaaagaa cccggattaa
     2161 acagacttga aaaggaagtt cttgccagag ttttggtcga gatatgcgat gctgacggta
     2221 atctttcagg tgaagaagct gaatttctta acaagattct tcctaaagaa ttgggaagtg
     2281 tcgaaaaaat cagaaatatg gattacatat caccattgga actaaaagct ttatctgaaa
     2341 atgccaagaa gcaaatagtc aaatgcgcct ttatactaag ctattcagac ttacaacctc
     2401 atgaaacaga aacaggaatc attcaggaat atgctacaca aatggggctt tcttcacaag
     2461 tgttacgcca aatggaaaaa gaagcacaaa aggagcttgt catgactatg attgacgaga
     2521 ataccacaag ggcagaagta tatgaaatcg gccgccaaat taatctatct gacgaagaat
     2581 ccgaaatgat actgattcac aagaaagaat ccatgtaaaa tgtactacct gaagcttccc
     2641 tat
//
LOCUS       DAOQQN010000386         2632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_21099_length_2632_cov_4.065192, whole genome shotgun sequence.
ACCESSION   DAOQQN010000386 DAOQQN010000000
VERSION     DAOQQN010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21099_length_2632_cov_4.065192"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PLG24_11920"
     CDS             <1..978
                     /locus_tag="PLG24_11920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HMZ25647.1"
                     /translation="VSVIADAIIKDEYRGDANAALDACVKTARNRKYEGLAYYMDLGF
                     IPEDKSSSSVSKVLEYAYDDWCIMKMAEKLGRLDIQQEFGKRSGNFRNVFDPTTGFMR
                     PKMSDGKFKKEFDPMDTHGQGFIEGNAWNYSLYVPHDVPGLIQLLGNKGRFEQYLDQL
                     FGKDLPDKYFEKTEDITREGIIGNYVHGNEPSHHVAYLYNWTDSPWKTQQKVRMILKN
                     QYRNGPDGLSGNDDCGQMSAWYLFSSIGFYPVCPGSDQYMLGSPLVESATIRLENGNI
                     FSVKTINQAPDNVYVKSITWKGFPWKERYITHEMIKNGGELVFEMSAKP"
     gene            1138..2562
                     /locus_tag="PLG24_11925"
     CDS             1138..2562
                     /locus_tag="PLG24_11925"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ25648.1"
                     /translation="MYRPVFFLFFVIFWQTNIYSQETWTLERCIQEAIANNIQIQQTD
                     LAVRRDEIGVTTARAGRFPSLNASTSFYESFGRQIDPTTNSFNSQNFGNQSYNLNSGI
                     TLFNFNYINNTIRQNVENLKASKADKSQMERDIALNVAQAYLVILVAKENLEVSRKSL
                     EQTNEQLTMTDKLIAAGSKNRNARLELLAQQANNEAQIITAENDVTIGFLNLKQLMNI
                     APDLSMDIAPPAIGLPMENPDEIDYQALISYSIKSQPKFEALEYKLRSALLGEKIARA
                     QGLPSIQAQASAGTSYSTLSQRILSTTLERQQIPGVLLNDEPVSLSFLNPTVTTGKNP
                     YFNQLNQNLGFGLGVGLSIPIYNRNQVKASVQNARLNTENVRLQESQAKQQLLSDILR
                     ALTDAQAARRNLDAANKNYDALNGAYNDTQKRFEIGAANTFELVSAKNNLDNAHRNTI
                     IRKYDYIFKMKVLDYYAGRQIILE"
BASE COUNT          821 a          556 c          605 g          650 t
ORIGIN      
        1 gtatccgtca tcgcagatgc catcatcaag gatgaatatc ggggagatgc caatgcagcg
       61 ctggatgcct gtgttaagac cgcaagaaac agaaaatatg aaggcctggc ttattatatg
      121 gatctgggct ttattcccga agataagtcg tcatcatctg tgtcaaaagt actggagtat
      181 gcctatgacg actggtgcat catgaagatg gcagaaaagc tcggcaggtt ggatatccaa
      241 caggaattcg gcaagaggtc cggtaatttc agaaatgtat ttgatccaac aacaggcttc
      301 atgagaccaa agatgtcaga cggtaagttc aagaaagagt ttgacccgat ggacactcat
      361 ggtcagggct tcatagaagg gaatgcctgg aattatagcc tttatgtacc tcacgatgtg
      421 ccgggactaa tccaattgtt aggtaataag ggacgttttg agcaatacct cgatcaactt
      481 tttggaaagg acctgcctga caagtatttc gagaagaccg aagacataac ccgtgaaggc
      541 atcatcggca actatgtaca cggaaatgaa ccgtcccacc acgtagccta cctttacaac
      601 tggactgata gcccatggaa gacccagcag aaagtaagga tgatccttaa aaatcaatac
      661 agaaacggac ccgacggctt gagtggcaat gatgactgtg gccagatgag tgcctggtat
      721 ttattctctt ctatagggtt ttatccggtg tgccccggtt cggatcagta tatgctgggc
      781 agtccattgg tggaaagcgc cacaattcgg ttagaaaacg gaaatatttt cagcgtaaaa
      841 accatcaacc aggccccgga caatgtatat gtcaaaagta tcacctggaa gggcttcccc
      901 tggaaagaga gatacattac ccacgaaatg ataaagaatg gaggggagct ggtatttgag
      961 atgagcgcca agccatgatg gttactttag gaaaagtatt gggacaaaaa tagccaaacg
     1021 gggctaaatt tatttcgtaa tataaaactg gcatttcacg acatattaaa ttgatttggt
     1081 agattttttt ttgataaaca gctcttgtaa cttatcattg ttgaaaaatt ttaggttatg
     1141 tacaggcctg tattcttcct gttcttcgta atattttggc aaacaaatat ttatagtcag
     1201 gagacatgga cattggagcg ttgcatccag gaggcaatcg ctaataacat ccagattcag
     1261 caaaccgacc ttgctgtcag acgtgatgag ataggtgtca ccactgcccg ggcaggccgg
     1321 ttccccagcc tgaatgcaag tacgtccttt tatgagagtt ttggccgaca gattgacccc
     1381 accaccaata gtttcaatag tcagaatttt ggcaaccaat cctataatct gaattccggt
     1441 ataacgttgt ttaatttcaa ttatatcaac aataccatcc gccagaatgt ggaaaatcta
     1501 aaggcttcta aagcggacaa gagtcagatg gagcgtgata tagcccttaa tgtagcacag
     1561 gcttaccttg ttattttggt agcgaaggag aatcttgaag tgtcacgaaa gagcctcgaa
     1621 cagaccaatg aacagttgac gatgacggac aagcttatag ctgcgggatc aaagaatcgc
     1681 aatgcccgac tggaattact tgcccaacag gcaaataatg aggcgcagat tatcactgcc
     1741 gaaaatgatg tgaccattgg ttttctcaat ttgaaacaac tgatgaatat tgctcccgat
     1801 ctcagtatgg atattgctcc tccagctatc ggcctcccga tggaaaaccc ggacgaaatc
     1861 gactatcaag cattaatctc atattcaatc aagtcacaac caaaattcga agccctggaa
     1921 tataagcttc gcagtgcctt gttgggagaa aaaatcgcca gggcacaagg gcttccttcc
     1981 attcaggccc aagcatctgc agggacaagc tattcgacat taagccaacg catcctgagc
     2041 actacccttg aaagacaaca gatacctggt gtcctgctca atgatgagcc ggtcagcctg
     2101 tcatttttga atccgacggt gacaacaggg aagaatccct atttcaatca gttgaatcaa
     2161 aaccttggtt ttggtcttgg tgtcggccta agcataccga tctacaaccg caatcaggta
     2221 aaagcttctg tgcaaaacgc cagactaaat accgaaaacg tcaggctcca ggagagtcag
     2281 gctaaacaac agctgctttc ggatatatta agagcattga cagatgctca ggccgccaga
     2341 aggaatctgg acgctgccaa taaaaactat gatgcgctga atggcgctta taacgacaca
     2401 caaaagcgat ttgagattgg cgctgccaac accttcgagt tggtatcggc caaaaacaac
     2461 ctcgataatg cacatcgaaa taccatcatc aggaagtacg attacatatt caagatgaag
     2521 gtattggatt attatgcagg gcgacaaatt atcctagaat agaatttatc aatgaaaaaa
     2581 agatatggct aagaaaagca agcgtttttg gtggttgata ggccttggag gc
//
LOCUS       DAOQQN010000387         2602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_21481_length_2602_cov_4.160581, whole genome shotgun sequence.
ACCESSION   DAOQQN010000387 DAOQQN010000000
VERSION     DAOQQN010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21481_length_2602_cov_4.160581"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="PLG24_11930"
     CDS             complement(<1..1501)
                     /locus_tag="PLG24_11930"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HMZ25649.1"
                     /translation="MKFLRFLTSLLIIIFCHFNQLFAQVYIEPGVQRELKEKGFSSCV
                     LAFQPLILKDKIPANRSKTEKGNTAYNLLKNHLKDNILEACTILDKSKIKYRINYLSS
                     TINAYHCPEAIVIKLAGLTNVKAVLYNYSFDGIKGDFQENSGGNRDSYTWGIIKTEAN
                     VIQELGYRGQGAVVGGNDTGFDWTHPAITTRYRGYNNGGVDHNYNWHDAIHEVSPLSN
                     PNQNNPCGLSLTAPCDDHSHGTHTVGTMVGVKEDNEVIGMAPDAKWIGTRNMERGNGS
                     LQSYLEALDWFLAPTDTNDLNPDPAKSPHVINNSWYCSEEEGCNVDNWNLMQVSIANL
                     KAAGIVVVASAGNSGPRCESIAFVPGMLDEVFSVGATNQVDTIAGFSSRGLVTADGSN
                     RLKPDIAAPGVGVLSSVLNHQYSYSSGTSMAGPHVAGLVALMVGANPDLAGQVDTIQE
                     IIKRTADPLTLDQTCNGLSGLDIPNPIYGHGRINAFKAVTAAINHGTNKT"
     gene            1564..2223
                     /locus_tag="PLG24_11935"
     CDS             1564..2223
                     /locus_tag="PLG24_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25650.1"
                     /translation="MKYLPEAIEFLYQQDAGWAVLVELTSIPEFGVPEPPYIELMSSI
                     VSQQLSTKVAKVIWNRFVDLFPRGFPEPSLVIELSDLQLREIGFSNAKANYVKNIAGH
                     FLAAPYTRDSLEAMDDLELLKEFSSIKGVGEWTVQMLQIFNLHRPDILPVKDLGVRQA
                     YSNFYGLNLTERELILHMNEKAEKWSPYRSAASLLLWNWKNHQFPGLEEIQKLNKNSK
                     N"
     gene            2226..>2602
                     /locus_tag="PLG24_11940"
     CDS             2226..>2602
                     /locus_tag="PLG24_11940"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HMZ25651.1"
                     /translation="MNLRHPHNIFGEEKRIERVIGRYDEGVKGPLVICIGGIHGNEKA
                     GLKAIDLMLKMLEVEHITNTNFEFKGRFLGVSGHLAAIRQNQRYIEKDLNRLWLKDKI
                     EWLSQQNRNTLSAEDLEMFDLES"
BASE COUNT          743 a          498 c          568 g          793 t
ORIGIN      
        1 ctgtcttgtt tgtaccatga ttgattgcag ctgtgactgc tttgaatgca ttgattcttc
       61 catgaccata tatcggatta ggaatatcca gaccggaaag tccgttgcaa gtttgatcga
      121 gggtaagtgg atcagcggtc cttttaatta tttcctgaat ggtatctacc tgacctgcca
      181 gatccggatt cgcacctacc attaaggcga caaggcctgc tacatgtgga cctgccatgc
      241 tggttcccga agaatagcta tattgatgat tcaggacaga ggataataca cctactccgg
      301 gtgcagcgat atctggtttg agtctgtttg aaccatctgc agttactaaa cccctactac
      361 taaagcctgc tatggtatcg acttgattgg ttgcaccgac tgaaaacact tcatccagca
      421 ttcccgggac aaatgcgata gattcacaac gtggtcctga attgcctgct gatgccacta
      481 ccactatgcc ggcagctttt agattggcaa tggagacttg catgagattc cagttgtcta
      541 cattacaacc ttcttcttca gaacaatacc agctgttgtt gatgacatgt ggtgatttgg
      601 caggatccgg attgagatcg tttgtatctg ttggagccaa aaaccaatcc aaagcttcaa
      661 gataagactg gaggctaccg ttgccgcgtt ccatatttct ggtgccaatc catttagcat
      721 ccggagccat tccgataact tcattatctt cctttacacc caccatagtg cctacggtat
      781 gtgtgccatg actgtggtcg tcacagggag cggtaagact caatccgcaa gggttgtttt
      841 gattgggatt actcaaagga ctaacttcat gaatagcatc atgccagttg taattatgat
      901 caacacctcc attgttataa cccctgtatc tggtggttat cgctggatgt gtccagtcaa
      961 atcctgtatc attacccccg acgaccgcgc cctggccccg gtaacccagt tcttgtataa
     1021 catttgcttc tgtcttaata atcccccatg tataagagtc tctgttgccg ccagaatttt
     1081 cctgaaaatc tccttttatt ccatcaaatg aatagttata aagtacagcc ttgacatttg
     1141 tcaatcctgc aagcttgata acaatagctt caggacaatg ataggcatta atggtggaag
     1201 atagatagtt gatacggtac tttattttgc ttttatccag tattgtacag gcttccagga
     1261 tattatcctt aagatgattt tttaaaagat tgtaggccgt atttcctttt tccgtttttg
     1321 aacgattggc cggtatctta tcctttaaaa tcaatggctg gaatgccagg acacatgaag
     1381 agaagccttt ttccttcaat tctctttgta cacccggctc gatatacact tgagcaaaca
     1441 gttgattaaa gtgacaaaag ataataatga gcaaagaggt aaggaaacgt aaaaatttca
     1501 ttgacttgat tgattagttt tcaaagatag ctacatttta aaataaatat atagctttga
     1561 caaatgaaat atttacccga ggctattgaa tttctttatc agcaagatgc cggatgggct
     1621 gttttggttg aattgacctc cattcctgaa tttggtgtgc ctgaaccacc atacattgaa
     1681 ttgatgtcgt ccattgtttc acaacagttg agtaccaaag tggcgaaagt aatatggaat
     1741 cgttttgttg acctttttcc ccggggattt cctgagcctt cccttgttat agagttgagt
     1801 gatctgcaat tgcgtgagat tggattttca aatgcaaagg caaattatgt aaaaaatata
     1861 gccgggcact tccttgctgc accgtataca agggattctt tggaggctat ggacgatctg
     1921 gaattgttga aggagttttc ttccatcaag ggtgtaggcg aatggacagt tcagatgttg
     1981 cagatattta acctacatcg tccggatatc ttgcctgtca aggatttagg agtaaggcaa
     2041 gcttatagta atttttatgg attgaatctt acggagcgtg aattgatatt gcatatgaat
     2101 gaaaaagcgg aaaaatggag tccatataga agtgctgcct cattattatt gtggaattgg
     2161 aagaatcacc aatttcccgg attggaagaa atacagaagc tcaataaaaa ttctaaaaat
     2221 tgaacatgaa tttaagacat cctcataaca tctttggtga agaaaaaagg atagagcggg
     2281 tgataggacg ctatgacgag ggtgtaaaag gtccactggt aatctgtatc gggggcattc
     2341 atggaaatga aaaggctggt ttaaaagcca ttgatttgat gctgaagatg cttgaagtcg
     2401 aacatatcac taatactaat tttgaattta agggtcgatt tcttggtgtg tccggacatc
     2461 ttgccgcgat cagacagaat caaaggtaca ttgaaaagga tttgaacaga ttatggttga
     2521 aggataagat tgaatggtta agtcagcaaa atagaaatac tttgtcggcg gaagatctgg
     2581 aaatgtttga ccttgaatca tt
//
LOCUS       DAOQQN010000388         2424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_24177_length_2424_cov_5.555509, whole genome shotgun sequence.
ACCESSION   DAOQQN010000388 DAOQQN010000000
VERSION     DAOQQN010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24177_length_2424_cov_5.555509"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLG24_11945"
     CDS             complement(<1..287)
                     /locus_tag="PLG24_11945"
                     /inference="COORDINATES: protein motif:HMM:NF014584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HMZ25652.1"
                     /translation="MRSIMMVGIGSAPGSLSRYGIVLMGKIFQWQLPGIPTFIANIIG
                     CFLFGILITLALKENIIIEEIKLFLSVGFCGGLTTFSTFTAENFTYLNQKI"
     gene            complement(346..1209)
                     /locus_tag="PLG24_11950"
     CDS             complement(346..1209)
                     /locus_tag="PLG24_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ25653.1"
                     /translation="MGRLHSLNYRKSSCIYLVRQYSILKVIRTTNRTFHVLLYSIGFL
                     FFSYKSNGQSEQKMNVYFISGLGADQRVFQKLTIDPEFNPVPIEWVKPGRKESMPSYA
                     RRLSTQIDTTKPFQIVGLSFGGMIAAEIADQIHPSQIILISSKSTSEPIGKFYRGLIH
                     LGLLSPFASLLLRSANRFTYKAFGAKTTEEKTMLRSILKDTDPKFMKWAIKTISSWSH
                     PKKSPIAYQINGSADRMIKANMVQYDYLVLGGEHLMIYSMASQISKILNERLKYGLHQ
                     SMSKSISNELK"
     gene            complement(1377..2117)
                     /locus_tag="PLG24_11955"
     CDS             complement(1377..2117)
                     /locus_tag="PLG24_11955"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HMZ25654.1"
                     /translation="MNFNKLILLTFLTLCNIHSVISQNDFFSKAKKAAKEITSGNTTS
                     LSNDEVIAGLREALTIGAQNSAGLASKVDGFYKNPAIFIPWPSEARNMRDKLIALGMS
                     KQIEEFERSLNRAAEEASKSAIPIFTDAIKKMSLNDGFNILKGADTAATHYLRVNTTD
                     GLTEAFSPVVANAIDKVQVTSYWTPLVNAYNKIPFVKKQDPDLKMYVTHKAIDGLMKL
                     LADEETKIRTNTSARVTDLLKKVFGNNN"
     gene            complement(2219..>2424)
                     /locus_tag="PLG24_11960"
     CDS             complement(2219..>2424)
                     /locus_tag="PLG24_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25655.1"
                     /translation="REERYTPMKNTEKSMPDKQSNQPSHRQPAPQRMQRDAQPGKPMN
                     SKMERQRSRSSDSRQGIPSSRRG"
BASE COUNT          641 a          444 c          490 g          849 t
ORIGIN      
        1 attatttttt ggtttaggta tgtaaagttt tctgcggtaa atgtcgagaa ggtggtcaga
       61 cctccacaga atccaaccga aaggaagagc tttatttctt caattattat attctccttt
      121 agtgccagcg taatcaggat gccgaataga aaacaaccaa ttatatttgc tataaaggtg
      181 ggtattccag gcagttgcca ttggaatatc tttcccatta gtacgattcc atatcttgaa
      241 agacttccgg gtgcactgcc tattccaacc atcattattg atctcatgga gtaatattaa
      301 gaaaaaattc atattgccaa gtcaaaatta aattagcgga taattttatt tcaattcatt
      361 agatattgac ttggacattg attgatgcaa tccatatttc agtctctcat tgagaatttt
      421 agatatttgg cttgccattg aataaatcat caggtgttct ccaccaagga cgagataatc
      481 atattggacc atgttggctt tgatcatcct gtcggcactt ccattaatct ggtatgcgat
      541 aggtgatttt ttaggatggc tccaagaaga aatcgtttta attgcccatt tcatgaactt
      601 aggatccgta tctttcagaa tagagcgcag catggtcttt tcctctgttg tttttgctcc
      661 aaaggcctta tatgtgaatc tattggctga tctcagaagt aaagaggcaa atgggctgag
      721 caggcccagg tggataagtc cgcgatagaa ttttcctatg ggctcactgg tacttttgct
      781 agatataagg atgatttgac tgggatgaat ttggtcggca atttcagctg ctatcattcc
      841 tccaaaggaa aggcctacga tctggaatgg cttggttgtg tcaatctgag tacttaacct
      901 tcgcgcatat gaaggcatcg attccttgcg tccaggcttg acccattcaa ttgggaccgg
      961 attaaactca gggtcaatag tcagtttttg gaacaccctt tgatcggcgc caagaccact
     1021 gatgaaatag acattcattt tttgttcaga ttgtccattg gatttgtagg aaaagaacaa
     1081 gaaaccaata gaataaagca aaacatgaaa tgtccgattg gttgttctta taaccttcag
     1141 tatggagtat tgccggacaa gataaataca gcttgatttt ctgtaattta aactatgtaa
     1201 tctgcccatc atatgtgagt tttcacaata ttacgcatac aatggaaaaa attatcaaag
     1261 ttttgagttt cttccaaata tttgttgatg aagattaatt tttcaaaaat atttaattcg
     1321 tcctttattt ttcttccaga gttgtgtgga taagatattt cgatggattg ttccgcttaa
     1381 ttattatttc caaagacttt tttgagcaag tcggttaccc gggcggatgt attagtcctg
     1441 atcttggttt cttcgtcggc cagaagcttc attagaccgt cgatagcctt gtgtgttaca
     1501 tacattttca aatcaggatc ctgctttttt acaaacggta ttttattgta ggcattgacc
     1561 aatggagtcc aataggatgt tacctgtact ttgtcgattg cgttggcgac gactggacta
     1621 aaggcttcag taaggccatc agtcgtattt acccttaagt agtgggtggc agctgtatca
     1681 gctcctttca gaatattgaa tccatcattg agactcattt ttttaattgc atccgtaaat
     1741 attgggatcg cgcttttgga tgcttcctca gcagctctgt taagtgatct ctcaaattcc
     1801 tctatttgtt tgctcatacc taaggctatt agtttatctc tcatatttct tgcctcggaa
     1861 ggccagggga taaatatggc aggattttta taaaagccat cgactttaga ggctaatccg
     1921 gcagaatttt gagcccctat tgtcagtgct tctctaagac ctgcaattac ctcgtcatta
     1981 gacagggaag ttgtgttacc ggacgtaatt tccttggcag ctttttttgc cttactgaaa
     2041 aaatcatttt gtgaaattac tgaatggata ttgcacaggg tgagaaatgt taatagtatt
     2101 agcttattaa aattcattct ggtatatttt gctatctaag atcaataaat gatgggaacg
     2161 gtctcattat taaaaccgtt cccttgtatt caataattat ttaaaccttt tgtttcattt
     2221 agccacgcct ggatgacggt attccttgtc tcgagtcaga ggaccttgat cgttgtcgtt
     2281 ccattttgct gttcattggt tttcccggtt gtgcatctct ttgcattctt tgaggtgcag
     2341 gttgcctgtg tgagggttga ttagattgct tgtccggcat acttttttct gtatttttca
     2401 ttggggtata cctttcttcc cttt
//
LOCUS       DAOQQN010000389         2388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_24781_length_2388_cov_3.762538, whole genome shotgun sequence.
ACCESSION   DAOQQN010000389 DAOQQN010000000
VERSION     DAOQQN010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24781_length_2388_cov_3.762538"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PLG24_11965"
     CDS             <1..505
                     /locus_tag="PLG24_11965"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ25656.1"
                     /translation="ATPNPVQFGGTLQLLATGGTNYLWTGPNGFYSTQPDPVIHRVNK
                     NHAGVYAVIISNAGGCELTLFVNVQVVNPKNGGDNQIIVTTTKIVEGKIYPNPANSDV
                     TLQRHTDAPVVYSIIDMQGRVIVRNATSEQGKVSIEELVPGVYNVMWSQPGIVGDAFK
                     GSFIKIR"
     gene            complement(1148..2032)
                     /gene="ygiD"
                     /locus_tag="PLG24_11970"
     CDS             complement(1148..2032)
                     /gene="ygiD"
                     /locus_tag="PLG24_11970"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HMZ25657.1"
                     /translation="MDRKDFLRMMGILTGASVMQPLSILSRAADLHGVTDRMPVIFFG
                     HGSPMNAIEKNEFSETWKDLGRKIEKPTAILCISAHWETAGTYVTAMDQPRTIHDFGG
                     FPKELYAIKYPAPGNPTLAEKIKSGIQSTNIGLDMSWGLDHGCWSVIKHLYPEADVPV
                     LQMSIDYTKGPEYHFALAKELASLRRKGVMIVGSGNIVHNLSMVAWDKMDISGYAYDW
                     ATEANNKMKSSILSGDYKPLLEYNKQGQAFRLAIPTPEHFIPLLYILALKESDENPFL
                     FNDVAMMGSLTMTSVLIK"
BASE COUNT          675 a          475 c          494 g          744 t
ORIGIN      
        1 agctacacca aatccggtac aatttggggg aactctgcaa ttgcttgcta caggtggcac
       61 aaactatctg tggacaggac cgaatggatt ttactctaca caaccggatc cggttatcca
      121 ccgtgtcaat aaaaatcatg ccggcgtcta tgcagtcatc atcagtaatg ctggtggatg
      181 tgaactaact ttgtttgtca atgtccaggt tgtaaatcca aagaatggcg gagacaatca
      241 aataattgtg acgacaacta agatagtaga gggcaagata tatccgaatc ctgccaacag
      301 tgatgttaca ttgcagcgtc atactgatgc acctgtagta tattccatca ttgacatgca
      361 aggtagggtc atagtgagaa atgcaacttc agaacaaggt aaggtatcta ttgaagaact
      421 tgttccgggt gtttacaacg tgatgtggtc acaaccgggt atagtcggtg atgcctttaa
      481 gggtagtttt atcaagatca gatagaaccc acattcaata atgattttga tagaggcggc
      541 attttgccgc ctctattctt ttggcctctt gcggtcagga aaataccgtg ttttcacggt
      601 gcttttatcg ttttttaccc gatcaatgat atacttaaag gtccgatatt tgtgttgtta
      661 cgacaagcag gatgactaag gatttcagga tttgactctt cttttccata tttaaccaca
      721 ggacgatgtt ccttttgttc gaggggccca ttatgagttg agccaaattg atgattacta
      781 agaattgcca aaattattga atactcgctt gcctggatat cttcggataa tgattcggat
      841 tcggcaatag tttaattcac cgaaatttca gagtaaggag atttagtata ggtggaaact
      901 tgaggtggaa agtctttaac aaccatccat ttttttggac aactaatctt tcaaaaattt
      961 gtaacaatgt taaaggcgtg tcgttaagcg gattctattt gataagtatt ttaaaaccat
     1021 gtttaatatt agacaatgtc tcaaaacaaa ttaaagacac cttactctct ccaacaatta
     1081 tattagcttt tccgctcgtg gcctgacctc cctggatggc cacttttttt tattattctt
     1141 cgatcattta cttgatcagg actgatgtca tggtcagtga acccatcatt gcaacatcat
     1201 tgaacaggaa aggattttca tcgctttctt tcaaagcaag aatatacaat aaagggataa
     1261 agtgttccgg ggtgggaatg gcaagtcgga aagcttgacc ttgtttgtta tactccaata
     1321 aaggcttgta gtcacctgaa aggatgctgg atttcatttt attgtttgcc tctgttgccc
     1381 aatcataagc atacccggaa atatccattt tatcccatgc aaccatgctc agattgtgta
     1441 cgatatttcc acttcctacg atcattacgc cttttctgcg taaggatgcc agttcttttg
     1501 caagggcaaa atgatactca ggccctttgg tatagtcgat actcatctgt aagactggta
     1561 catctgcctc ggggtaaaga tgttttatga ctgaccaaca accatgatcc aaaccccagg
     1621 acatatccag cccaatattt gtggactgga ttcctgactt aatcttttct gctaaagtgg
     1681 gatttccggg tgccggatac ttaatagcat acaactcttt ggggaaaccg ccaaaatcat
     1741 gaatggtcct gggttgatcc attgctgtta cataggttcc ggctgtctcc cagtgtgctg
     1801 aaatgcataa tatcgctgtc ggtttttcaa tcttccttcc taggtctttc caggtttcag
     1861 aaaattcgtt cttctcaatg gcattcattg gacttccatg gccaaagaag ataacaggca
     1921 tacggtcagt gacaccatgc aggtcggcag cccgggatag tatgctcaga ggctgcatga
     1981 cgctcgcacc ggtcaatatt cccatcattc gtaaaaaatc ttttctgtcc aactaataat
     2041 tgaattaaat gtagccatta aacaatagag agtgagattg gttgacagaa tccggccaaa
     2101 tagcctgtga ttccgcctat tgattttatt gatcttttag atttacctta agataaccgc
     2161 tatttgggaa taccttttca ggcaagttca gacatggaga caatacaaac cctggtgtgt
     2221 tgatttagaa aaatattaag tactggttag cttgttacgc catgctttca ctgggtcgat
     2281 atcagtggtt aagaccttta tgtagaggta agtacaagta cagtaattcc atattttgaa
     2341 attttgtaaa tcccacagtc taagcttatc gtattgcggc ctgtaatg
//
LOCUS       DAOQQN010000390         2366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_25171_length_2366_cov_6.115534, whole genome shotgun sequence.
ACCESSION   DAOQQN010000390 DAOQQN010000000
VERSION     DAOQQN010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25171_length_2366_cov_6.115534"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1709
                     /locus_tag="PLG24_11975"
     CDS             <1..1709
                     /locus_tag="PLG24_11975"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HMZ25658.1"
                     /translation="TIQAVHDSSYFHPPMPDLIEREKPDKANGSNNWALAGSKTMTGR
                     PILCNDPHLKLRLPSIWYEIQITTPEYSCYGVSIPGAPGILIGFNEYVAFGSTNAMRD
                     VMDYYEITFRDKSKSQYLYNGRWESTSSRIETYVIRDAKTQADTVFYTHFGPVVYDEN
                     FRGLKNDHLRSNAGKFYAVRWKAHDPSNDIVSFLLLDRAKNYDDYRKAIARFSCPGQN
                     LIFASASGDIAITQQGVFPAKWRYQGEFIMEGKDSLYEWQGMIPQDQVLTMKNPERGF
                     VSSANQLPADPKEYPYFLGGDYVYERGYRINQILRKLQKATVQDMIDLQNDNFNIRAH
                     QIRGLLIETLKKRNFTNEADSYFHILERWNLYNDSASEGATVYSLLMDSLEQRIWGDE
                     LKKSQIGSMPEHSTLIQNLLRNINFACVDNVLTPQVETFDEQLSAAFLSIVPVLDELK
                     KVGRLTWGKYKQTCVPHLMDIKQNMSSLSRLDLNVGGGDGIVNATKKSHGPSWKMIVS
                     LTSPVEAWGVYPGGQSGHPGNPYYDTGVGPWSEGKYFKLWFMKPFEKGSDKIIQTLVF
                     KP"
     gene            1759..2124
                     /locus_tag="PLG24_11980"
     CDS             1759..2124
                     /locus_tag="PLG24_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25659.1"
                     /translation="MNIIKYLIFIIGTLVVSALLTYIGPWWLIFVGPLLFAFFFKISY
                     GKSFLVGAFSIMLFWAIMLLIRSDVFVGSITEKIALLLPLRGSRVALSLVTLVVGGVI
                     GGLAGLNGRSWRRSFGLTQ"
BASE COUNT          670 a          422 c          567 g          707 t
ORIGIN      
        1 taactattca ggcggttcat gattcgtcat acttccatcc gccaatgcct gatttaatag
       61 aaagagaaaa gccggataag gctaatggaa gcaacaattg ggcacttgcc ggaagcaaga
      121 ctatgaccgg cagaccaatt ctttgcaatg acccgcacct taagcttcgc ttgccttcca
      181 tttggtatga aattcagata acaacacccg agtattcatg ctatggggta tccatacccg
      241 gtgcgccagg tatattgata gggttcaatg agtatgtagc tttcggttct accaatgcca
      301 tgcgagatgt gatggattat tatgaaataa cattcaggga caaatcaaag agtcaatatc
      361 tctataatgg ccgttgggag tccacttcga gcaggataga aacctatgtt ataagggatg
      421 caaagactca ggcagatact gtcttttata ctcattttgg ccccgttgtt tatgatgaaa
      481 atttccgtgg attgaagaac gatcatttgc gttccaatgc tggcaaattt tatgccgtgc
      541 gctggaaagc acatgatcca agcaatgata tagtatcatt tcttttgttg gacagggcga
      601 agaattatga tgattaccgg aaagccattg ccagattttc ttgtcccggg caaaacctga
      661 tttttgcttc ggcttccggc gatattgcca ttacccagca aggtgttttt cctgcgaagt
      721 ggaggtatca aggtgaattc atcatggaag gaaaggattc actgtatgaa tggcagggaa
      781 tgatacctca ggatcaagta ttgaccatga aaaatccgga acgaggattc gtaagttctg
      841 ccaatcaatt acctgcagat ccaaaggaat acccctattt tctgggtggt gactatgtat
      901 atgagagagg ataccggatt aatcagatac tgcgcaaact tcaaaaggcg actgttcagg
      961 atatgattga cttacagaat gataacttca acatcagggc gcaccaaatc aggggtcttt
     1021 tgattgaaac tctgaaaaaa aggaacttta ccaatgaggc cgacagctat ttccacattc
     1081 tggaaagatg gaatctgtac aatgatagtg ccagtgaagg agcgactgtc tattctcttc
     1141 tcatggacag tttggagcaa cgaatatggg gagacgaatt gaagaagtct cagataggaa
     1201 gcatgcctga gcatagtaca ttgattcaaa atctgttgcg aaacattaat ttcgcatgtg
     1261 tagacaatgt tttgactcct caagttgaaa catttgatga gcaactgtcc gctgcatttt
     1321 tatccatagt tccagttttg gacgaattga aaaaagtcgg tcggctgact tggggtaaat
     1381 acaagcaaac ctgtgtacct cacctgatgg atatcaaaca gaatatgtct tcattgagca
     1441 gacttgacct aaatgttgga ggcggagacg gaattgtcaa tgcaacaaag aagagtcatg
     1501 gtccaagttg gaagatgatt gtctcgctta cctcccctgt tgaggcgtgg ggcgtgtacc
     1561 ccggcgggca atccggtcat ccgggcaatc cttattacga taccggggta ggaccttggt
     1621 ctgaagggaa atactttaaa ctttggttca tgaaaccctt tgagaagggt agtgataaaa
     1681 tcattcagac attagtattc aaaccatgag agtattcaaa ccactatatc tatcagtaca
     1741 aattacaatt agacagaaat gaatattatt aaatatctga ttttcattat tggcacattg
     1801 gttgtttccg ctttgttaac ctatattggt ccttggtggt tgatttttgt agggccttta
     1861 ctttttgcat tcttttttaa gatatcatat ggcaagtcat ttttagtagg agcattttca
     1921 ataatgcttt tttgggctat catgttgtta attagatctg atgtttttgt cggatcgatt
     1981 actgaaaaga ttgccttgtt attgccgttg cgtggttcac gagtagcatt aagtttggtt
     2041 actttagtgg tcggaggtgt gataggtggg ttggccgggt taaatggcag atcttggcgt
     2101 agaagtttcg gtttgacgca gtaaatgata atcatccggt gaaggctaac aggaagagag
     2161 gtaagggaga tggggtgtag gtgtgggtta ggaaagatga agtgagtaga agattaatag
     2221 ccaccatgct tgacggtatt gcctttcggg gcggctttgc caaaattcag gtaatttata
     2281 caggtgttta attttgcatt tgcttccaaa cccttgtgta tggaggtttt tgaattatta
     2341 ctttcctacc tacttcgatt tggggg
//
LOCUS       DAOQQN010000391         2333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_25717_length_2333_cov_4.660667, whole genome shotgun sequence.
ACCESSION   DAOQQN010000391 DAOQQN010000000
VERSION     DAOQQN010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25717_length_2333_cov_4.660667"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="PLG24_11985"
     CDS             complement(<1..1045)
                     /locus_tag="PLG24_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HMZ25660.1"
                     /translation="MNKKFSPEVKKIITQSRDEALRLGNNYIGTEHLLLGLLADRESK
                     AVKVLASMDVDFGELCKLLEESAVEKGHASGGITAGNVPLDKHAEKVLKVTFLEAKLF
                     KTDEISPEHLLLSILKHKDNIASRILKKYDIDYESFRTELDYLGNPDSVEDWPELGNM
                     SAGSGDDYSMEDEGISRYQKKGSSKSKTPVLDNFGRDITRLAEEGKLDPIIGREVEIE
                     RVSQILSRRKKNNPILIGEPGVGKTAIIEGLALRILKKKVSRTLFNKRIVMLDLAALV
                     AGTKYRGQFEERIKAIMNELEKTRDVILFIDEIHTIVGAGGATGSLDASNIFKPALAR
                     GELLPERGGDGAAD"
     gene            1441..1824
                     /locus_tag="PLG24_11990"
     CDS             1441..1824
                     /locus_tag="PLG24_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ25661.1"
                     /translation="MKYSIDKQEHYAIMTLEEENLNSLLAPDLKSEFVIFKNEGITNL
                     VINLSNVKYIDSSGLSSILTGNRLWKENGLFILTEINHVAVKKLIEISRLDSILTIIP
                     TLEESLDYVMMDEIEKELNSSTEEE"
     gene            1927..>2333
                     /locus_tag="PLG24_11995"
     CDS             1927..>2333
                     /locus_tag="PLG24_11995"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ25662.1"
                     /translation="MDCGEGTIFRLNDLKLSKMKIEAIFISHLHGDHVYGLPGLVTSL
                     ILMGRKDSLTIIGPIGIKKMMEQVLESSWTRLNYPLEFIETNPEKQEIVFYTPAYTVE
                     TIPLKHKIPCNGYLFRQQQKPKNIREEIVLAYQL"
BASE COUNT          740 a          513 c          381 g          699 t
ORIGIN      
        1 cgtccgccgc gccatcgccg ccccgctcgg gcagtagctc tccccgagca agcgccggtt
       61 tgaaaatatt ggatgcatcc agcgaaccgg ttgcaccacc agctcccaca atcgtatgaa
      121 tctcatcgat aaataaaatg acatctctgg tcttttccaa ttcattcatg attgctttaa
      181 tacgctcttc aaactggccg cggtatttcg ttccagcaac caaagcagca agatccaaca
      241 taacaatcct cttgttgaac aaagttctcg agaccttttt cttaaggatt cttaaggcaa
      301 ggccttctat gattgctgtt ttacctacac cgggctcacc gatcaggatt ggattgtttt
      361 tcttgcggcg actcaaaatc tgagaaacac gttcgatttc tacttcccga ccaataatgg
      421 gatccaactt accctcttcc gcaagtctgg ttatatcgcg accaaaatta tccaatacag
      481 gtgttttgga tttggatgaa cctttcttct gatatctgga gataccttca tcctccatag
      541 agtaatcatc accacttcct gcggacatat tgcctaactc aggccaatct tctaccgaat
      601 ccggatttcc taaataatcc agttctgtcc tgaaactttc gtaatcgata tcatactttt
      661 ttaggattct ggaagcaata ttatccttgt gcttgagaat actcaacaaa agatgctcag
      721 gactaatctc atccgtcttg aacagcttgg cttcaaggaa ggttacctta agcactttct
      781 ctgcatgctt atccaatgga acattgcctg cagtaattcc tcctgaggca tgaccttttt
      841 caacagcaga ttcttcaagc aacttgcaca actctccaaa atcaacatcc atggatgcaa
      901 gcaccttaac cgctttgctt tcacgatcgg ccagcaagcc aagcaataag tgttcggtac
      961 caatataatt attacccaac cttaaagctt catccctact ttgggtgatg attttcttaa
     1021 cttcaggtga aaatttctta ttcattatta ttactcctac ttattgtttt tatgacaata
     1081 gtcaaaaatt tattactaat atacgctaaa agtagtcaaa tagtttaatt cgatacaatt
     1141 atagcgattt atcactttat aaaaaaataa aatatgatat tttatttcat ctccaaagga
     1201 taatgaaaaa atcattccat aataatttga cgctaataaa ctaaaaaagg cacaaatgta
     1261 gaccaacctt attcagacct ttccttcaat actctttaat tcagctcaaa taggcctcag
     1321 gtaatatgca gaaaacttca ttttactttc aaataaaata aaatcgtata tttgtcagtt
     1381 ttttaattta acaaacttta acttcctgct gacaaaatta cataaatagc attatatcgc
     1441 atgaaatatt caattgataa gcaagagcat tatgccataa tgacactgga ggaagaaaat
     1501 ctcaattccc ttcttgctcc cgatttgaaa tccgaattcg tcatttttaa aaatgaaggc
     1561 attacaaatc ttgtcatcaa cctatccaat gtaaaatata ttgacagtag tggattaagc
     1621 tccattctca ccgggaacag gctatggaaa gaaaatgggc tttttattct tacggaaata
     1681 aatcatgttg cggtaaaaaa gttaatcgaa atatcaaggc tagactctat cctcacaatc
     1741 ataccaaccc tggaggaatc tttggattat gtcatgatgg acgaaattga aaaagaactg
     1801 aattcgagta cggaggagga ataattgagt ttcaacgtaa ctatattagg ctccaattcg
     1861 gcattgcctg cccatggccg ccacccttca tcacaattat tgaccataga ccatcatgaa
     1921 atccttatgg actgtggaga gggtacaata tttcgattga atgatttgaa attgtcaaaa
     1981 atgaagattg aagctatctt catcagccat ctccacggcg accatgttta tggacttccg
     2041 ggacttgtta ctagcctgat actcatggga aggaaagatt ctttgactat aattgggccg
     2101 attggtataa aaaaaatgat ggaacaggta cttgagtctt cgtggacccg actcaattat
     2161 ccccttgaat ttatagaaac caatcccgaa aaacaggaaa ttgtattcta cacaccggca
     2221 tataccgttg agactattcc cctgaaacat aagatccctt gcaatggata tttgtttcga
     2281 caacaacaaa agccaaaaaa tatcagagaa gaaattgttt tggcctacca act
//
LOCUS       DAOQQN010000392         2279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_26764_length_2279_cov_6.547662, whole genome shotgun sequence.
ACCESSION   DAOQQN010000392 DAOQQN010000000
VERSION     DAOQQN010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26764_length_2279_cov_6.547662"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PLG24_12000"
     CDS             <1..820
                     /locus_tag="PLG24_12000"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HMZ25663.1"
                     /translation="LAIVAISFTSCKDDDNKLPPIDGYNNADEVASTNLVAYWGLNGD
                     GKEAKSSTSPSKSDKSSFVTGKKGQALDLAGGYLLYPEIASLNSLKSFTVSLWANGLK
                     NNGSHASILFSLGRANEWAGSINIMAETGWRKAGDDTLTIKGLIVSNAANGASWQDSR
                     NDIAKGGDQVFTKGNQWSQYVISYDATTEKFKVFANGVKVSNPDWETRTGLGELKVFT
                     PSIPIIGAWWTNTGGTPDSWQVPFTGQIDEIRIFNKALSDAELSALYKLESAGR"
     gene            1517..>2279
                     /locus_tag="PLG24_12005"
     CDS             1517..>2279
                     /locus_tag="PLG24_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HMZ25664.1"
                     /translation="MKRVVITGIGAITPIGNNIEQYNQGLKNGVSGAGPITRFDAANF
                     KTRFACEVKDFNPEDFIDKKEVRRMDLFTQFAMVCSDEALADSKLLEYTELDYDRIGV
                     IWGSGIGGLRSLEAEIETFARGNGIPRFNPFMIPKMISDIAPGYISIKHGFRGINYGT
                     VSACASATHAMMNAFDYIRLGRADVIITGGSEAAITATGMGGFSATKALSERNDDFLT
                     ASRPFDLERDGFVLGEGGGCLIFEDYEFAKKRGAKI"
BASE COUNT          643 a          433 c          533 g          670 t
ORIGIN      
        1 attggcaatt gtagcaatca gtttcacttc ttgtaaagac gatgacaaca aattaccccc
       61 gattgacggt tacaacaatg ccgatgaagt agccagcacc aatctggttg cctactgggg
      121 tttgaatgga gatggtaagg aagccaaatc ttccaccagt ccttcaaaat cagataagtc
      181 atcatttgtt actggtaaga aaggtcaggc actggatttg gcaggtggat atctcctgta
      241 ccctgaaatt gcctccctaa acagcctcaa aagcttcaca gtttcccttt gggcaaatgg
      301 cctgaagaat aatggaagcc atgcaagcat cttgttcagc cttggcagag ccaatgagtg
      361 ggcaggatcc atcaatatta tggcggagac aggttggcgt aaggcaggtg acgacacatt
      421 gacgatcaaa ggcctcatag tttctaatgc tgcgaatggg gcaagttggc aggatagcag
      481 aaatgatata gcaaaaggtg gcgatcaggt gtttaccaaa ggtaatcagt ggtcacagta
      541 tgttatctcc tatgatgcaa caactgagaa attcaaagtt tttgccaatg gagtgaaggt
      601 aagcaatcct gattgggaaa caaggaccgg tttaggtgaa ttgaaagtct tcacgccatc
      661 tattccaatc atcggagctt ggtggaccaa cactggcggc acaccggatt cttggcaggt
      721 accgtttacc ggccaaatcg atgaaatccg tattttcaat aaggcgcttt cagatgccga
      781 gttgagtgca ctgtacaaac tggaatctgc aggaagataa aatcttatca ttcagaaaat
      841 taaggcacct tttacgggtg ccttttttta ttttcgaaac tttttggaca ttccacaata
      901 ccttgagggt tttatcggtt gatgatggat cccatgacaa ttcctacgaa taaatggatg
      961 gtgtggtttt ggattttttt catttgttta atttatttca ctacaatggt atttgtgtgt
     1021 tgtaatttcc aaccggactt tcatttatac aatacagcaa cacattattt agaagaatat
     1081 tcaaggtatt ttgttgaatc ggccaaattg aataaatttc atatggtctt tgatgttgag
     1141 acaagctctc cggagctaaa ggcagtataa tcctatgatg cctctccgcc gttatttagt
     1201 tttttagctc cggtattgga gagtgcaccc tcaatctctt actatttggt ctgattttgc
     1261 cactgaacaa tcaaaggatt gttgtggagg ggcgacttga ttgaaataag atatttttat
     1321 tcgattgact cttattataa tttgattctt ttacctcatt tgccgtccga ttatagattc
     1381 tatttcaaaa tgttatagac acaaggcata cagtaatagc tactttgcaa atatgttatt
     1441 atatttgcct cgcagaagtt cgcttttcga aatgaatagt catgaggctc tctgcaccag
     1501 acaaagctat aaacttatga agagagtcgt aattacaggc ataggtgcca ttactcccat
     1561 cggaaataac atcgagcaat acaatcaagg tctgaagaat ggtgtaagcg gtgccggccc
     1621 tattacccgg tttgacgctg ccaatttcaa gacccgtttt gcctgtgaag tgaaggattt
     1681 taatccggag gatttcattg acaagaagga ggttcgtaga atggatttat ttactcaatt
     1741 tgcaatggta tgttctgatg aagcattggc tgacagtaag ttattggaat atacagagct
     1801 tgactacgac aggattggcg taatttgggg atccggaata ggaggattgc gttctttgga
     1861 agcggaaatc gagacatttg cacgaggaaa tggtatccct cgttttaatc catttatgat
     1921 acccaagatg atctccgata ttgcacccgg gtatatcagc atcaagcacg gtttcagagg
     1981 cattaactac gggacggtat ctgcctgcgc ttcggccacg catgccatga tgaacgcctt
     2041 tgattatatc cgactgggca gggccgatgt catcattacc ggaggttctg aagcggcaat
     2101 aactgcaaca ggtatgggcg gttttagtgc aacgaaggct ctgagtgaac gtaacgatga
     2161 cttccttacc gcatccagac ctttcgacct ggagcgggat ggctttgtat tgggcgaagg
     2221 tggaggttgt ctgatttttg aagattatga atttgccaag aaaagagggg ctaaaattt
//
LOCUS       DAOQQN010000393         2230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_27742_length_2230_cov_5.179310, whole genome shotgun sequence.
ACCESSION   DAOQQN010000393 DAOQQN010000000
VERSION     DAOQQN010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27742_length_2230_cov_5.179310"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="PLG24_12010"
     CDS             complement(<1..1526)
                     /locus_tag="PLG24_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HMZ25665.1"
                     /translation="MSQVNNLADTLKERNRGHQIFQRIAAWPGRIFSTELNPMYQLGG
                     LAVLMFVIACISGIYIFIFYNINPHQAYESVEAISNNYFNGWMRNIHRYSSDLLVVFI
                     LLHLIHTLFTSKFKRLISWISGVISFLVVITIGVTGFILIWDQKAKLTGYLTAKLFSG
                     LPIFDPSIMGAFLLNDLSVVGGFFKVALFGHIALSLITVIIIWIHVLRISKPKIFPPG
                     KLILYASIALGIIAILFPVKSDPPAQMTNLPAETTFDWFYYFGYYLMKVFSVGQNWAI
                     MIGSGLLLSILPFMLRRKDRSPVHIDLDKCDACNLCSYDCPYGAIDMLIYNGERKAIL
                     SPDKCVGCTICIGSCKEHAITHPNFPVFDFSPDPKQDLTVFSCSFFPEVTFPSELNTT
                     QYKVPCLGSVMVKDVQKIMDGGTTKVAMLGCEDCYYRYGKNWAVERLLKKRPPAYSRR
                     LDTSGIRLFTINQFSPDRLLEFCREQPDENTGEAKIMDYGRARPILSTLMLAAFFALF
                     "
     gene            complement(1501..>2230)
                     /locus_tag="PLG24_12015"
     CDS             complement(1501..>2230)
                     /locus_tag="PLG24_12015"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HMZ25666.1"
                     /translation="LILSGFRMPEEERHLGRKIPDITIMKADGSRSNLYSLLRGKPLV
                     LTPIYTKCPSICGLISNGTKEAVTALGTLGRDFNMVSFSFDSTDTPDNLSYYQQRWKM
                     DGETWQTVTSDGKSIRSLLASIGYEYDRDTVLKQFNHPALLVVLTPSGRISRFIYGVN
                     PTKRDIDLAVMAAMAEQTRPGLFKGFYLRCFGYDPLTRTYKIDWRFIISTSAGIIIIF
                     FVTSIFLKTFIFSKPEHVSSEQSG"
BASE COUNT          684 a          503 c          467 g          576 t
ORIGIN      
        1 ataaacaagg caaagaaggc tgcaagcatc agtgttgaga gtatcggtct ggccctccca
       61 taatccataa ttttggcttc cccggtattc tcatccggct gttctctgca gaattccaat
      121 aatctgtcgg ggctaaactg atttatagta aataatctta tcccggaagt atccaacctt
      181 ctggagtatg ccggaggtct ttttttcaat aatcgctcca ccgcccagtt tttaccgtat
      241 cggtaatagc agtcttcaca accaagcatg gctaccttgg tagtgccacc atccatgatc
      301 ttttgcacat ccttgaccat gacgcttcca agacaaggca ccttatattg agtagtgttg
      361 agttctgaag gaaatgtaac ttcagggaag aagctacaag agaacactgt caaatcctgc
      421 tttggatccg gtgaaaaatc aaagacagga aagttggggt gtgtaatggc gtgttcctta
      481 caagagccta tacagattgt acagcctaca catttatcag gagaaagaat cgctttcctt
      541 tcgccattat aaatcaacat atcgatcgcc ccatacggac aatcatagga acatagatta
      601 cacgcatcgc atttgtcaag gtcgatatgc accggcgacc tgtccttcct tctgagcata
      661 aatggcaata tactcagcag caggcccgaa cctatcatga tggcccaatt ctgacctaca
      721 ctgaatacct tcatcaggta atacccaaag taatagaacc agtcaaaagt agtctcagca
      781 ggcaaattgg tcatctgggc cggtgggtcg ctctttacag gaaacaggat tgcaatgatg
      841 cccaatgcaa tggaagcata caaaatcaat ttcccgggtg gaaaaatttt aggcttcgaa
      901 atcctcagaa catgaatcca tataatgatg accgtaatta aagaaagagc aatatgtcca
      961 aatagagcta ccttgaagaa acctccgact acagaaagat cattgagcaa gaaggcaccc
     1021 atgatggaag ggtcaaagat gggaaggccc gaaaaaagct ttgctgtcag ataacctgtc
     1081 aatttagcct tttgatccca tattaaaata aagcccgtta caccgatagt aatcactacc
     1141 aaaaatgata tcacaccact tatccatgaa ataagccttt tgaactttga agtaaacaaa
     1201 gtatggatca ggtgtaataa tataaataca accaacaaat cggatgagta tctgtggata
     1261 ttacgcatcc agccattaaa ataattattg gaaatcgcct ccacggactc ataagcctgg
     1321 tgcggattaa tattataaaa aataaatata taaatgcccg atatacaagc tattacaaac
     1381 ataagcactg ctaatccgcc cagctgatac atgggattca gttcggtgga aaatatcctc
     1441 cccggccagg cagctatcct ttggaagatc tgatggcctc tattccgctc ttttaaagta
     1501 tcagccagat tgttcacttg agacatgttc aggttttgag aaaataaagg tttttaaaaa
     1561 gattgatgta acgaaaaaaa tgatgataat tccggcggat gtagaaatga taaagcgcca
     1621 gtcaatctta taagtgcgag tcaatggatc gtatccaaaa catcggagat agaatccttt
     1681 gaacaggccc ggcctggttt gttccgccat agcagccatg acagcaagat caatgtcgcg
     1741 tttagtgggg tttactccat agataaatcg ggagattctg ccggatggtg tcagaaccac
     1801 caacaaagcc gggtggttga attgtttcag gactgtatcc ctatcatact catatccgat
     1861 ggatgcgagg aggcttcgga tcgacttccc gtcggacgtg accgtctgcc aggtctcacc
     1921 gtccattttc caccgttgtt gataatagct cagattgtcc ggagtatctg tactgtcaaa
     1981 ggaaaagctg accatattga agtcccgccc cagcgtaccc aaagctgtca cagcctcttt
     2041 cgtaccattg ctgatcagac cgcaaatcga agggcacttg gtatagattg gggtaagcac
     2101 taatggcttc cctcttagca gcgagtaaag attggaccta ctgccatcag ctttcatgat
     2161 ggtgatatcg ggaattttcc tgccaagatg tctctcctcc tcaggcattc tgaatccgga
     2221 aagaatcagc
//
LOCUS       DAOQQN010000394         2204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_28300_length_2204_cov_5.681712, whole genome shotgun sequence.
ACCESSION   DAOQQN010000394 DAOQQN010000000
VERSION     DAOQQN010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28300_length_2204_cov_5.681712"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PLG24_12020"
     CDS             <1..773
                     /locus_tag="PLG24_12020"
                     /inference="COORDINATES: protein motif:HMM:NF018648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HMZ25667.1"
                     /translation="MQSLLAGLGFAVAMFVGNKMGVRNIFFYAILGICGVWLSFMNSG
                     VHPTIAAVIAAFTIPADVILPEKEYSGKIEKLLKQFNSQDLGDKTKALSQSQLHILDN
                     VKDQTNAAIAPLQRLEHALHPFVNLFVLPIFAFANAGVSLNVEMSGLFSGGVIPGVFL
                     GLLVGKVVGVVGTSYLMKAANLAVIPRGVNFRGMIGLGLLAGIGFTMSLFVTTLAFTN
                     PEHTDQAKIGIFTASITAGIAGYWLLKKVFTEPPASES"
     gene            complement(745..>2204)
                     /locus_tag="PLG24_12025"
     CDS             complement(745..>2204)
                     /locus_tag="PLG24_12025"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HMZ25668.1"
                     /translation="VGLTVLDTFLFLLLQNYGVRKMEAFIITLVAIIGVAFVIEIYLA
                     KPDIGEMAAGFIPSIPNSAALYIAIGIIGATVMPHNLYLHSALVQTRRFGKDEKSLRN
                     AIKFNFIDSAVALNLAFFVNAAILIVAAATFFKNGLHEVAEIQDAYVFLEPVLGSKLA
                     PILFAVALIAAGQSSTITGTLAGQIVMEGYLNIRIQPWLRRLITRLIAIVPAMLVIMI
                     YGEKETGPLLILSQVILSLQLGFAIIPLIHFVSDRNKMGVFTIGIKLKLAAWVIAIII
                     VSLNIRLVLNIFDEYYSSSTRMKDIILFLGIPLFVAAMTLLGYITIKPLLTKNKTTRI
                     TTPHGTIAPLNIQAAQQFKHIAIALDFSSIDSIAVSRALNEGGKAAKYLLIHIVESAG
                     AYVSGGDSHDRESSADKANLIAYAGHLTELGYQVEYCLEYGNPKKKIPLIVNEAGADL
                     LIMGAHGHQWHHDLLFGTTVDAVRHRIKIPMLVVR"
BASE COUNT          590 a          466 c          517 g          631 t
ORIGIN      
        1 ctatgcaaag cctactcgcc gggttgggtt tcgctgtagc catgttcgtc ggaaataaga
       61 tgggcgtccg gaatatcttc ttttatgcta ttttaggtat ttgtggtgtc tggctttcct
      121 ttatgaactc cggcgtacat cctaccattg ctgccgttat cgcggcattt accattcctg
      181 cagatgtgat cttacctgag aaagagtatt caggaaaaat tgaaaagtta ttgaagcagt
      241 tcaacagtca agacttaggt gataaaacaa aggcattgtc tcaaagtcag ttacatattc
      301 tcgacaatgt aaaggatcaa accaacgcag ctatcgcgcc tttacaaagg ctggaacacg
      361 cattacatcc atttgttaac ctgtttgttc tcccaatctt tgcctttgcc aatgcaggtg
      421 tcagtttgaa tgtcgaaatg tccggtttgt tttcaggagg agtgataccg ggagtatttt
      481 tgggcttact tgttggcaag gtggtcggtg ttgtggggac cagctatctg atgaaggcag
      541 cgaatcttgc tgttattccc aggggtgtta atttcagggg catgattgga cttgggttgc
      601 tggcagggat aggttttacc atgtctttat ttgtgacaac attggcattt acgaatcctg
      661 aacataccga tcaggcaaaa atagggattt tcacggcttc tattactgca ggtattgccg
      721 gttattggct attgaaaaaa gtatttaccg aaccaccagc atcggaatct tgatacggtg
      781 acgcactgca tcaacagtgg tgccgaagag aaggtcgtga tgccattgat gaccatgggc
      841 acccatgatt agtaagtctg cacctgcctc attgacgata agcggaattt tctttttggg
      901 atttccatat tccaggcaat actctacctg atagcccaac tctgttaaat gccctgcata
      961 agcgattaga ttggccttat cggctgatga ttctctgtca tgggaatccc cacctgatac
     1021 ataggcaccg gcggattcta caatatggat gagaagatat ttggccgcct tccctccttc
     1081 attcaatgcc ctgcttacag caatggaatc tatagatgaa aagtccaatg caattgcaat
     1141 gtgcttgaat tgttgtgctg cctggatatt cagtggagct attgtgccgt gtggagtggt
     1201 gatcctggtt gtcttgtttt tagtcaacaa tggtttgatg gtgatatatc ccaataatgt
     1261 cattgcagcg acaaataaag gaatccctaa aaagagtatg atgtccttca tccgggtcga
     1321 acttgaatag tattcatcga aaatattcaa gacaagcctg atattcaagc ttacaataat
     1381 tatcgctatc acccaggcgg ccaatttcag tttgatccct atggtaaata cgcccatttt
     1441 atttcgatcc gaaacgaagt ggatgagggg tataatggca aaacccagtt gaagagacag
     1501 aatgacctga ctcaggatga gcagagggcc ggtctccttt tctccatata tcataatgac
     1561 gagcattgcc ggcactatgg caatgagtct ggtaatcagc cgtcttagcc atggctggat
     1621 tctgatattg agatagcctt ccattacaat ctgacctgcc agggttccgg taatcgtgga
     1681 gctttggccg gcagcaatca aggccacagc aaatagaata ggagcgagct tactgcccag
     1741 caccggttca aggaatacat atgcatcctg tatttccgct acttcgtgca agccattttt
     1801 gaaaaatgtc gcagctgcaa cgatgagaat ggctgcgttg acaaaaaaag cgaggtttaa
     1861 ggccacggca gagtcgataa aattgaattt gatggcattg cgtaggcttt tttcatcctt
     1921 tccgaatctc cttgtctgca ccagggcgga atgaagatat aggttgtgcg gcatgacagt
     1981 cgctccaatg atgccaatgg caatatataa ggctgcactg ttgggaatgg atgggatgaa
     2041 gccggcggcc atttctccaa tatccggttt tgcaagatag atctctatca caaatgcaac
     2101 tccaatgatg gctaccagcg taataataaa ggcttccatt ttcctgactc cataattttg
     2161 taatagcaaa aacaaaaatg tatccagcac ggtgagcccc acac
//
LOCUS       DAOQQN010000395         2152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_29520_length_2152_cov_7.085837, whole genome shotgun sequence.
ACCESSION   DAOQQN010000395 DAOQQN010000000
VERSION     DAOQQN010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29520_length_2152_cov_7.085837"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..1387)
                     /locus_tag="PLG24_12030"
     CDS             complement(293..1387)
                     /locus_tag="PLG24_12030"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HMZ25669.1"
                     /translation="MYDIILSFITAFLLTYFAIPSIINIAKVKKLVDLPDERRSHDEA
                     TPSLGGIGIFGGLLFSVIMWTPFNVFGDLQYVLCSLVIIFLIGAKDDILPMSPNKKIL
                     GEIFAAVILVFKANVKITSLYGIFNIYDLHPVASALFSIFSILVIINAFNLIDGINGL
                     SGSIGTLIAVTFGTWFFLVQRIELSLVAFALAGSLIAFLKYNYTPAKIFMGDTGALIV
                     GLVCAVLSIKFIEFHKEAPESIYAFHAAPAVAIGIMIFPLYDTLRVFVLRVANKKSPF
                     YPDRKHIHHMLIDLGLTHMQATLILVTINALFIVFSFIFQDIGSFNLIMILLGMALTG
                     SIILKQLLNYKQKELRTSKSKSDKTLAGIK"
     gene            complement(1803..1991)
                     /locus_tag="PLG24_12035"
     CDS             complement(1803..1991)
                     /locus_tag="PLG24_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25670.1"
                     /translation="MKKRKCDIERLMHATALLQAKVDDYSPERISVSANSFGRLCSSH
                     SNGSPSKNNGLPEGGKSV"
BASE COUNT          658 a          428 c          445 g          621 t
ORIGIN      
        1 gggatttttt gtttggccat caatcatgtt gtatctgttt ccatcaaacc gatgtcattc
       61 cctctttgag tttccagagt ctttgagcgc gaagaaatgt tgttcgttat cggtaagtaa
      121 agagtgaaaa gagaatagtg cgggcacata taagtccgga gtgacaaagg attcacaggt
      181 aaaagtgcta aaaattcatt attaaagaca aatatattgg ttgatcaact acaagttgtg
      241 atgtggttgc ctttcacaaa aaaagattct gaaggtggct gtatcacatt tcttatttaa
      301 ttccggccag cgtcttatcg gattttgatt tacttgtccg taactccttt tgtttgtaat
      361 ttaataattg cttcaggata atacttcccg taagcgccat ccccaaaagg atcatgatca
      421 ggttgaagga gcctatatcc tgaaaaataa atgaaaatac tatgaacaag gcgttgatgg
      481 tcaccagaat aagggttgcc tgcatatggg tcaggcccag gtcaatgagc atatggtgta
      541 tatgcttccg gtccggataa aagggacttt tcttattagc tacacgcagg acaaatactc
      601 tcagggtatc gtacaatgga aatatcatga tgcctatggc aactgcaggt gcagcatgga
      661 atgcgtaaat cgattccggc gcctccttgt gaaattcgat gaatttaatg gataggacag
      721 cacaaaccag ccccacaatc aatgccccag tgtctcccat aaagattttt gctggtgtat
      781 aattgtactt taggaaagcg attagagaac cggctaatgc aaaagctacc agactgagct
      841 ctatacgctg gacaaggaag aaccatgtgc cgaaagttac ggcaatcaga gtccctatac
      901 ttccactcaa cccattgatg ccgtcgataa ggttaaaggc attgatgatt accagaatgg
      961 agaatatcga aaacaatgcg gaagctacag gatgcagatc atagatattg aatattccat
     1021 ataaggaagt tatttttaca ttggccttaa ataccagaat cacagccgca aatatctcac
     1081 ccaggatctt tttatttggt gacatcggaa ggatatcatc tttggctcca ataagaaaaa
     1141 taatcaccag cgaacaaagg acatactgaa ggtctccgaa tacattgaaa ggtgtccaca
     1201 taatgaccga aaacaacaaa ccaccgaaaa tgccaattcc tccaagggaa ggcgttgcct
     1261 cgtcatggga gcggcgctca tccggtaagt ccacaagttt tttgaccttc gctatattga
     1321 tgatagaagg tattgcaaag taggtcagca aaaatgccgt aataaaagat aatattatgt
     1381 cgtacatagg ttcggatttt acaaatttgc atttcttctt tttgcagaat gacgctaaat
     1441 tgtattccga gggcaaattt aatacaatta ccgacaggat gagcctgttt tcttatattt
     1501 ggaataattc tatgcttaaa attgtgttga taatgatcac tgattggcaa tcgacttctt
     1561 atttcttatt acccttatgc ttatcctgta aatgtgttat ctaactaata acgaagcggt
     1621 gtttgaagat gaccatcaga cgtgttcagt aatggcaatg aaagacaaat ctcccgggat
     1681 gtaagcacct ataatagctt gcagtgtatt cacctcacct ctatcaaaac gcagtaaaat
     1741 ccggtaaaat tttgccgaaa taatgatttg cacggatggg gggggtaaat ataggaagcg
     1801 tgttaaactg acttccctcc ttcaggcagg ccattatttt ttgatggact tccattggag
     1861 tgtgaagaac agagacgccc aaaactgttt gcagacacgg agatcctttc tgggctatag
     1921 tcatcaactt tggcctgcag taaggctgtg gcatgcatca atctttcaat atcacacttt
     1981 ctctttttca cgacgcgtta cttgcataca taagatttta tttattatct ttcaccttgc
     2041 aaagttccag ttgatcatga ccgactatca aaatccgaat cttgtcgtcc atcccggtaa
     2101 agtgcgctcc ggaaaaataa cctggcgcag tccgtccaat attgcgctga tc
//
LOCUS       DAOQQN010000396         2127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_30114_length_2127_cov_5.753861, whole genome shotgun sequence.
ACCESSION   DAOQQN010000396 DAOQQN010000000
VERSION     DAOQQN010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30114_length_2127_cov_5.753861"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLG24_12040"
     CDS             complement(<1..422)
                     /locus_tag="PLG24_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25671.1"
                     /translation="MESVSARVRRLFLQIILLFICYALCRALFMFVNRQELSIHDIGS
                     FAEVMLGGMRFDLSAITASNVLYIILLMLPLPFFASKVYRRLLAIIFMLTNGVCLLGN
                     LVDIAYFPFVHKRSQADALRFVTGEKGGDLFRLLPTFAA"
     gene            complement(537..1115)
                     /gene="infC"
                     /locus_tag="PLG24_12045"
     CDS             complement(537..1115)
                     /gene="infC"
                     /locus_tag="PLG24_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HMZ25672.1"
                     /translation="MAKKERQDKGAEKAQFRINEQIRIPEVRLIGEDFEVISDVAGHN
                     VEAGIYSTPLLLDWARQAGLDLVEISPNATPPVVKIIDFQKFLYNKKKKERELKAKTV
                     KTVIKEIRFGPNTDDHDFDFKVKHARNFLGEGAKVRAYVQFRGRSIVFQDRGELLLLK
                     FMKELEDVGAAEALPKLEGKKMGVVISPKKKK"
     gene            1538..>2127
                     /locus_tag="PLG24_12050"
     CDS             1538..>2127
                     /locus_tag="PLG24_12050"
                     /inference="COORDINATES: protein motif:HMM:NF025029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HMZ25673.1"
                     /translation="MPDPSVTDITTPAPPEEKKKDDTEILFETCGLNGIVSLDAFKEA
                     MVGYTRFQPMNSILTIVDFSLPSNEKRFFVIDLANKKLLLSSLVAHGQKSGELMATKF
                     SNKPNSHQSSLGFYRVGAKIFSPKHGDALSLDGLDKGLNDNARAREIIIHGADYVSDD
                     FIKKFGRLGRSFGCPALPRDIMAEVLPIIRDGSILF"
BASE COUNT          629 a          457 c          418 g          623 t
ORIGIN      
        1 gcagcaaagg ttggtaaaag acgaaacagg tcacctccct tttctccggt gacgaatcta
       61 agggcatctg cctgtgaccg cttgtgtaca aatggaaaat aagcgatatc gaccaaattg
      121 ccgagcagac acacaccatt ggtcaacatg aaaattatag caagtaacct tcgataaacc
      181 ttactggcga agaagggcaa cggcagcatc agcaggatga tatacagaac attagatgcg
      241 gtgatcgcag acaaatcaaa tctcattcca cccaacatca cttccgcaaa ggaacctata
      301 tcatggatac taagttcttg cctgttgaca aacatgaata atgcacggca taatgcatag
      361 caaataaaca gaagaataat ctgcaagaaa agtcgtctta ctctggctga aactgattcc
      421 atggagtaaa aattatccgg acgatgatct tccaaaattc agactcaaac cggcgcagtc
      481 gtcgaaaagt gattgagacc cgggcgatag tgtctgtcca tcagccgtca atacagttac
      541 tttttcttct tcggggaaat aacgacaccc atcttcttgc cttccaattt cggcagtgct
      601 tcggcggcac cgacatcctc cagctctttc ataaatttga gcaggagcaa ctctccccta
      661 tcctgaaaca cgatgcttct tcccctaaac tgaacataag ccctcacttt tgcaccttcg
      721 cccagaaaat tacgagcgtg ttttaccttg aaatcaaagt catggtcatc ggtattcggt
      781 ccgaatcgga tctccttgat aacagttttt actgtctttg ccttgagctc tctttccttc
      841 ttctttttat tgtaaagaaa cttctggaag tcaataattt tcacaacagg gggagtagca
      901 ttgggggata tttccaccaa atccaaaccg gcctgtctgg cccagtctaa aagcaatggc
      961 gtggaataaa tcccggcttc tacattatga cctgcgacat cacttatcac ttcaaaatct
     1021 tctccgataa gccttacctc aggaattctg atttgttcat tgattcggaa ttgagctttt
     1081 tcggctcctt tatcctgtct ttcttttttt gccaaattaa aaattaagat ttaaaataat
     1141 tatctgtatc gttttgtttg tggttgtaac agagctgatt aacgtaaaac agcggtattc
     1201 agttcctttc cggcacaaat gtaatttatt tttatttatc aggtatttat aggtatggga
     1261 aaagtgcata taccgttgat atattttaat tccggggaaa gaattgtggc gaagccaagc
     1321 ctgagcttga taaagagtat aaggggtgta tcaggaaggc caaatgagct aaaaaggagg
     1381 atgtagtttt taaatctgaa tttttttaac aaaccatttc aaaaaaatga caatctttgc
     1441 gtcaactttt tcatttttaa agaattgtaa aaggcattca agaaactaat ctatgtattt
     1501 attcattcgg ctcctatctc tttttttaat tgcaacaatg cccgaccctt ctgtaacaga
     1561 tatcacaaca cccgcccctc ctgaagaaaa gaaaaaagat gatacggaga ttctttttga
     1621 aacctgcgga ttgaatggta tagtttcact ggatgcattt aaggaagcga tggttggcta
     1681 tacgaggttt cagccgatga attcaattct caccatcgta gatttttccc tcccctctaa
     1741 tgaaaaaagg ttttttgtca tcgatcttgc caataaaaaa ttattgttgt catccctggt
     1801 cgctcatggc caaaaatccg gtgaattgat ggccactaaa ttttccaaca aacccaattc
     1861 tcatcaaagt agccttggat tctaccgggt aggagcaaaa atattcagtc ccaagcatgg
     1921 agacgcttta agcctggatg ggctggataa gggtcttaat gacaacgccc gggctaggga
     1981 aattataatc cacggggcag attatgtcag tgatgacttt ataaaaaaat ttggcaggct
     2041 gggtcgcagt tttggctgtc cggcattgcc cagagatatt atggccgagg tacttcccat
     2101 tatccgcgat ggttcgatcc tttttat
//
LOCUS       DAOQQN010000397         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_30566_length_2110_cov_6.841363, whole genome shotgun sequence.
ACCESSION   DAOQQN010000397 DAOQQN010000000
VERSION     DAOQQN010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30566_length_2110_cov_6.841363"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLG24_12055"
     CDS             complement(<1..399)
                     /locus_tag="PLG24_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25674.1"
                     /translation="MENTELIGIWKTQNEKLEKTLTINEILLKELINNKARTSLRSLI
                     KLKTAGIIAFVLYLLLLGNALAFAIAHYSSAWNYFIVSVAVIALVNVKGFADYIKHLV
                     WANNINYNGSVMQIQQQLSKLQLSIIDHARM"
     gene            complement(401..913)
                     /locus_tag="PLG24_12060"
     CDS             complement(401..913)
                     /locus_tag="PLG24_12060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMZ25675.1"
                     /translation="MNRSEQSELFLSVVHAHKGIIYKVVNSYCKNPEDREDLVQEIII
                     QLWKSFGQYDDSFKHSTWMYRIALNVAISFYRKESSRKRISNPMTEGIINFPEQDDFN
                     DTETEVGMLRSMIAQLKDLDKALILLYLDEKNHKEISRIMGISETNVATKINRIKNHL
                     KQKFAQLKKN"
     gene            complement(900..1097)
                     /locus_tag="PLG24_12065"
     CDS             complement(900..1097)
                     /locus_tag="PLG24_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25676.1"
                     /translation="MKKGVKANLAFAIIAISLFATGCGITVNRNNYYYYPYQSENKSI
                     MKTTYTDYLVKSQAYKCNEQI"
     gene            complement(1437..>2110)
                     /gene="aat"
                     /locus_tag="PLG24_12070"
     CDS             complement(1437..>2110)
                     /gene="aat"
                     /locus_tag="PLG24_12070"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HMZ25677.1"
                     /translation="GLLGLSDELRYEDLKTAYRFGIFPVNNPSEPILWWSPDPRWILL
                     SGQVFVSKSMRKILRDRPWRATCDTDFELTMHRCAEGKGRKDRHPSWITGEYIDVYKM
                     FFLDGRAHSFEVWDHSGVMIGGLYGVLTGSVFVGESMFSDVSNTSKYAFILACNFMHE
                     LGIEVIDCQMHSEHLESLGAIPVHRDWFLNYLKWNGFEVSGLMGSWEERFRVFMESTY
                     GNNAG"
BASE COUNT          577 a          452 c          402 g          679 t
ORIGIN      
        1 cattctggca tgatcaatta tcgataattg tagtttggac aattgctgtt gtatctgcat
       61 gacacttcca ttgtagttga tattgttggc ccatacaaga tgtttaatat agtctgcaaa
      121 gcccttgaca ttgaccaagg caattacagc aacggaaaca atgaaataat tccacgccga
      181 agaataatgg gcgattgcaa aggccagtgc attccctaaa agcaacagat agagtacaaa
      241 ggcaatgatg cctgctgttt tcagtttaat caatgatctt aaagaagtcc tggccttatt
      301 gttgatgagt tctttcaaca aaatctcatt gatggtaagc gtcttttcta atttttcgtt
      361 ttgggttttc caaatgccga ttagttctgt attttccatt ttaattcttt ttaagttgag
      421 caaatttttg ttttaaatga tttttgatgc ggttgatctt tgtggcgaca ttcgtttccg
      481 atattcccat aatacgggat atctctttat ggtttttttc atccagatag agtaggatca
      541 atgctttgtc aaggtctttg agctgagcaa tcatgctcct caacatacct acttccgttt
      601 ctgtatcgtt aaaatcatcc tgctccggaa agttgatgat tccttccgtc atcggattgg
      661 atattctctt cctgctactc tctttccgat aaaaagagat agcgacattc agggcaattc
      721 ggtacatcca ggtggaatgt ttaaaactgt catcatattg accaaatgac ttccatagtt
      781 ggataatgat ctcctgaacc aggtcttcac gatcttccgg atttttacaa tacgagttga
      841 cgaccttgta gataatacct ttatgggcat gaacaacaga aagaaagagt tcggattgct
      901 cagatctgtt cattacactt gtatgcttgt gatttgacaa gatagtcagt ataggttgtt
      961 ttcatgattg acttgttctc gctttggtaa ggataataat agtaattatt tctgtttact
     1021 gtgattccac atcctgtagc aaataacgat attgcgatta ttgcaaatgc caagttggct
     1081 tttacacctt tcttcatttt attcattgat tagctgttca aatttttatt tcctgattat
     1141 tcgcatcagc atcgtaaata tcacatttcg ggaatttttt tttggggcat tataattgac
     1201 cggggtagat attttgtaac tttttcgttc taatccaggc aattattacc aatgcaatcc
     1261 ccatcccaca taccttctaa atggtagtga tggttgaata aatagtcaaa aaaaagtaat
     1321 cagtactgtt tttagctgac agctattcta tccggatgta ggaagatgga tggtcatagc
     1381 gatcttggac caatcattgt ttttaccggg atagataatc ttcatgggtt aagaattcat
     1441 cctgcattat tcccgtacgt actctccatg aacacgcgga atctttcttc ccagctaccc
     1501 atcaggcccg acacttcgaa accattccat ttcagatagt taagaaacca atcccggtgt
     1561 acaggaatgg cacccaggct ctccagatgc tcggagtgca tctggcagtc aatcacctcg
     1621 atgcccaact catgcataaa gttacaagcc aggataaagg cgtatttgct ggtattggac
     1681 acatcggaaa acatgctttc gccgacaaag acacttcccg tcaatacacc atacaagccg
     1741 cctatcataa cccctgaatg atcccagact tcgaagctat gtgctctccc gtccagaaaa
     1801 aacattttgt atacgtcaat atattcaccg gtaatccagg aaggatgcct gtctttacgt
     1861 cctttccctt ctgcgcacct atgcattgtg agttcgaagt ccgtgtcgca ggtggcccgc
     1921 catggcctgt cccgaagaat tttcctcatg gacttactta cgaaaacctg tcccgaaagt
     1981 aagatccatc gcgggtcagg gctccaccac agtataggtt cgctgggatt gttgaccggg
     2041 aaaataccaa atcgataggc agttttcagg tcttcgtatc tcaactcgtc tgacaatccc
     2101 aataaaccat
//
LOCUS       DAOQQN010000398         2082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_31309_length_2082_cov_4.624568, whole genome shotgun sequence.
ACCESSION   DAOQQN010000398 DAOQQN010000000
VERSION     DAOQQN010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31309_length_2082_cov_4.624568"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="PLG24_12075"
     CDS             <1..701
                     /locus_tag="PLG24_12075"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ25678.1"
                     /translation="AKEKAAAIMASNMQLSKEDALGMISALRLTNHGDNINYFGLNNE
                     YKGITGEALYSKMGDKYHSLNLAPANRPNWRALSNSYAVSNSTLQPTGDNAGEGNARF
                     SPATASAKTAPAVATKTVSITFPTASYTLDENAKSLIDLQFADVAKGFGNSRVRIEGN
                     TDNVGDKLKNVELSRKRAEAVASYLENTYNMDKNRFVIIGNGMDKPVVGCESNASPEC
                     RAKNRRTEFQLIGE"
     gene            790..1860
                     /locus_tag="PLG24_12080"
     CDS             790..1860
                     /locus_tag="PLG24_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMZ25679.1"
                     /translation="MNLFELRGALPPKVKLILEIAGILLLLFVWFLLTLGTEPIVPAG
                     ILPSPSKVLNAIGLVFIENELVVNLCRSLGLNICGYLEAIIISVVFGFLVGLIPLFNG
                     LFAKPIDAFRFVPLTGVIGLFIVWFGLGVPMKVHFLAFGILIYMLPVIVTRINDVEDV
                     YLKTAYTLGATKWQIVRTVYIPHVMSKFSDDIRVLTAISWTYIIIIESIGNEGGLGAL
                     MWRTGIRQGNIAKLFAMLFIIIIIGFVQDKLFAMLDRRLFPFKYLKESVKASGKLTED
                     NKLRLILSIIKPYFVLLVAGIIIILLANEFIGFLGRKGILSYAFGETYPIIAVLMSVI
                     SLWIVFDFVNFRKVPKKSLEKA"
     gene            1862..>2082
                     /locus_tag="PLG24_12085"
     CDS             1862..>2082
                     /locus_tag="PLG24_12085"
                     /inference="COORDINATES: protein motif:HMM:NF015057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25680.1"
                     /translation="MTVDLLKEMEKDLLVKIEKILNHPVITKIHNGELTKEQLRLFAI
                     QYSIYCGYFPRFLCACAANIPDDNTRMAI"
BASE COUNT          585 a          417 c          454 g          626 t
ORIGIN      
        1 cagcaaagga aaaggcagca gccatcatgg cttccaacat gcaattgagc aaggaggatg
       61 ccttgggtat gatcagtgcg ctgagactaa ccaaccatgg agacaatatc aattattttg
      121 gcctcaacaa tgagtataag ggcatcacag gcgaagcact ctattccaaa atgggtgata
      181 aatatcattc actgaatctc gcccctgcaa accgacctaa ttggagagca ttgtcaaaca
      241 gctacgctgt gtctaactct actcttcagc caaccggtga caatgccgga gaaggaaatg
      301 ccagattttc tcccgcaaca gcttccgcca aaaccgctcc tgcagtggct accaaaactg
      361 taagcatcac ctttcctacg gccagttata cccttgacga aaatgccaaa tctttgattg
      421 acctgcaatt tgcagatgtg gcaaaaggtt tcggtaactc aagagtcagg atagaaggaa
      481 atacggacaa tgtcggtgat aaactgaaaa atgtagaatt gagccgtaaa cgtgcagagg
      541 ctgttgccag ctatcttgaa aatacctata atatggataa aaaccgcttt gtcatcatag
      601 gtaacgggat ggacaagcct gttgtaggtt gtgagagtaa tgcctctcct gaatgccgtg
      661 caaagaacag acgcactgag tttcaactaa tcggagagta aaagatctat agtcaaaagg
      721 caggagttga tcctcctgcc ttttttcttg cattcaatcc cttattgtgg tcaataaaat
      781 acacttttga tgaatctttt tgaattgcga ggcgctctcc cccccaaagt aaagttgatc
      841 ctggaaatcg ccggtattct actgttgctg tttgtgtggt ttttgctgac tttgggtacg
      901 gaaccaattg tacctgccgg tatcctccct tctccttcaa aggtattaaa cgcgataggc
      961 ctggtgttta tcgagaatga attggtcgtc aacttatgca ggtcattggg attaaatatt
     1021 tgcggatatc tggaagcaat catcatatct gtagtctttg gatttctggt aggccttatt
     1081 cctctgttca acggactttt tgccaaacct attgatgctt ttcgtttcgt ccctttgaca
     1141 ggtgttatag gactttttat tgtttggttc gggttaggcg tacccatgaa agtacatttc
     1201 ctggcatttg gtatcctgat atacatgctg cctgtcatcg taaccagaat caatgacgtc
     1261 gaagatgttt atttgaaaac agcctataca ctcggggcaa ccaaatggca aattgtacgt
     1321 acagtctata ttccacacgt aatgtcgaaa ttttcagatg atatcagagt gttgacagcc
     1381 atttcctgga catatatcat catcattgaa agtattggca acgagggtgg gcttggcgcc
     1441 ttgatgtggc gtaccggaat cagacaaggc aatatagcca aattgtttgc catgctcttc
     1501 attattatca tcataggttt tgttcaggac aaattatttg ctatgttgga ccgacgactt
     1561 ttccctttca aatatctgaa agagtcagtt aaggcctcgg gaaagctcac ggaagataac
     1621 aaactgcgat taatactgtc gatcatcaag ccttattttg tgcttttggt ggcaggaatt
     1681 attattatct tactggctaa tgaattcatc ggctttctgg gtagaaaagg tatattgtca
     1741 tatgcattcg gtgagactta cccgataatt gccgtattga tgtctgttat ttccctttgg
     1801 attgtctttg attttgtaaa ttttaggaag gttcccaaaa agagcctgga aaaagcttga
     1861 tatgactgtt gacttactta aagagatgga gaaggatctt ttggttaaaa ttgaaaagat
     1921 tctgaatcat cctgtgatta cgaagatcca taatggcgaa ttgactaaag agcagcttcg
     1981 gttgtttgcc attcaataca gtatatattg cggatacttc ccaagattcc tgtgtgcttg
     2041 tgctgcaaat atacctgatg ataatactcg gatggctatc at
//
LOCUS       DAOQQN010000399         1900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_36731_length_1900_cov_6.643902, whole genome shotgun sequence.
ACCESSION   DAOQQN010000399 DAOQQN010000000
VERSION     DAOQQN010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36731_length_1900_cov_6.643902"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..623)
                     /locus_tag="PLG24_12090"
     CDS             complement(33..623)
                     /locus_tag="PLG24_12090"
                     /inference="COORDINATES: protein motif:HMM:NF037630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25681.1"
                     /translation="MHLHNRVNGRELKARMRQSDEKRITISFYKYHKIENPQFLRDHL
                     YLEWAKLGVLGRIYLANEGINGQISVPEESHEAFKAAFEQLDFMRGIRLNYAVDDDGK
                     SFFKLKILVRKKIVADGLNDSTFDVTNSGIHLSAKEMNELLEQSNTVVVDMRNHYESE
                     VGHFKNAILPDVTTFRESLPIISEMLAEHKDKNLVM"
     gene            complement(1072..>1900)
                     /locus_tag="PLG24_12095"
     CDS             complement(1072..>1900)
                     /locus_tag="PLG24_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ25682.1"
                     /translation="VDANGDLVHVGLGYRDPAGFIELGKEANDPARQYASLKKQYEAG
                     KRDPKLVATYMQLLAEMQDPAGDAVAAEYFKSQSDLTTPENVKLLASFAMKPTSPNHH
                     LVLENKEVLARQLGSDFLNELAYYYFIPNFRKGASMEDAVTQVTTIYPEKADILTFYT
                     KQYYTRSKKDMKGYEEALFNYLTPEHLDQFSSSELNSYAWYVYESTEGADKAKAAIPW
                     AEASVAKKSEYANNDTLAWLYFKAGDVERAKEIARKAIELGKAAGEDTSSTEELLQK"
BASE COUNT          470 a          424 c          405 g          601 t
ORIGIN      
        1 attgatccat taccctaata tttttcttgt atttacatga caaggttttt gtctttgtgc
       61 tcagccagca tttcacttat gataggcagt gattcccgga aggtggtcac atcgggcagg
      121 atggcatttt tgaagtgtcc gacttcgctt tcataatgat tgcgcatatc gaccaccact
      181 gtatttgatt gctcgaggag ttcgttcatt tcttttgcgc tgaggtggat gcccgagttt
      241 gttacatcga aggttgaatc attaagtcca tctgcaacaa ttttctttct tacaagtatc
      301 ttcaacttga aaaaggattt gccgtcatca tctacggcat aattcaatct gataccacgc
      361 ataaagtcaa gttgttcgaa agccgctttg aatgcctcat gactttcttc cggaacagat
      421 atctgtccat tgatgccttc attagccaaa tagattctcc ctaagacgcc cagtttggcc
      481 cattccagat acagatggtc ccgcaaaaat tgtgggtttt caattttgtg gtatttataa
      541 aatgatatag taatcctttt ttcatcggat tgtctcatcc tggcctttag ttcacggcca
      601 ttaaccctgt tatgtaaatg catttctctg ttcgtttgat taaatattaa cgcttaagca
      661 gcgattgggt tgattttcag ggcaaaaata caaaatgtgt ggaaatagtg attctaccgg
      721 atggttattc ctgagatttt ggcctatatc tctgatctgg cctatgcgtc attacatact
      781 tatggatttg tatctctcag gctcaaatgt agtactgaat ttcccaatgt ctaattgcaa
      841 taccgggcga attacttttt cgggaattga attcacctaa ctcatgaatc cccatgaatt
      901 taatatttac accggagata taatgctggc cggaacaatc cgggtgacag tatcatccat
      961 gcacaatcct tctaaagaca aatgactcaa tgaggcacat gttgaagggt tgtagtcgcc
     1021 tttcaatttc aggaacttcc taaaacatcc cggcagcatt catcattact tttatttctg
     1081 caacaattcc tcggtagaag aggtatcctc accggcagcc ttgcccagtt cgattgcctt
     1141 cctggctatt tcctttgccc tttctacatc accggccttg aaatataacc aggcaagggt
     1201 gtcgttattg gcatattcgc tttttttggc gacggaggct tccgcccatg gaatagctgc
     1261 cttggccttg tcagctcctt cggtactttc atatacatac caggcatagc tgttgagctc
     1321 ggaggatgaa aactgatcga gatgttccgg ggtgagatag ttaaacagag cttcttcata
     1381 acctttcata tcctttttgc tgcgtgtata atactgcttt gtgtaaaagg tcaggatatc
     1441 tgccttttca ggataaatgg tagtgacctg agttacagca tcctccatgc ttgcaccttt
     1501 cctgaagtta gggataaaat agtaataagc cagctcattc aggaagtccg atcccagttg
     1561 cctggccagc acctctttat tttccagcac cagatggtgg ttgggcgatg taggcttcat
     1621 agcaaaggaa gcgaggagtt ttacattttc cggggtagtc aggtcggact gtgacttaaa
     1681 atactcggca gcaacagcat ctccggccgg atcctgcatc tctgccagca attgcatata
     1741 agtggcaacc aatttgggat ctcttttgcc ggcttcatat tgtttcttta aggaagcgta
     1801 ttgccgggcg ggatcgttgg cttctttgcc cagttcaatg aagcctgcgg ggtcgcgata
     1861 gccaaggcca acgtgaacca ggtcaccatt ggcatcgaca
//
LOCUS       DAOQQN010000400         1621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0417
            NODE_48953_length_1621_cov_7.661558, whole genome shotgun sequence.
ACCESSION   DAOQQN010000400 DAOQQN010000000
VERSION     DAOQQN010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810049
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:55:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,398
            Genes (coding)                    :: 2,397
            CDSs (with protein)               :: 2,397
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48953_length_1621_cov_7.661558"
                     /isolate="AMR_MDS_0417"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PLG24_12100"
     CDS             complement(<1..804)
                     /locus_tag="PLG24_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HMZ25683.1"
                     /translation="MAEITNLKNKIARIKILRQLFFTPGLTSSEISNAIDKSLPVTTQ
                     LLTELIQEGRIEETGLAGSTGGRRPQTYSLADGSFQILSVAMDQLVTRIALVDEKARV
                     VGTRQLFELNLYKNPDSLSELIECIRLFINSENVDTSRILGIGIGMPGFVDVKKGINN
                     SFLPSAEGSVIEIIEKAIGLPVWIDNDSSLIALAESKWGAAKDKKHVMVLNIGWGIGL
                     GMLLNGKLYRGQEGFAGEFSHMSLFENDKLCNCGKYGCLETEASLLVTLE"
     gene            1248..>1621
                     /locus_tag="PLG24_12105"
     CDS             1248..>1621
                     /locus_tag="PLG24_12105"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HMZ25684.1"
                     /translation="MFFFLIYLIPGNTQEIISIIPKPVKLERKAGSFILDESTGYRQA
                     ADSGINQAVRLLTSECTRRSGINLKNTTKASKFISLAIVPGIIPEREGYTLSITPSAI
                     TLKAGDESGLIHGVQTILQLLPA"
BASE COUNT          493 a          353 c          284 g          491 t
ORIGIN      
        1 ttcgagcgtc accagcagag acgcctcagt ctccagacat ccgtactttc cacagttgca
       61 caacttatca ttttcaaaca atgacatatg gctgaactct ccggcgaatc cttcctgacc
      121 tctgtacagc ttaccgttaa gtagcattcc aagaccgata ccccagccga tgttgagtac
      181 catgacatgt ttcttatctt ttgcagcacc ccatttggac tcagcaagtg caatcaggct
      241 ggaatcgttg tcaatccaca ccggaagacc tattgccttc tctatgatct caatgacaga
      301 tccttcagcc gatggcagaa atgaattgtt gatccccttc ttgacatcta caaaaccggg
      361 cattccaatt ccaataccaa gaatcctgct tgtatcaaca ttctcagaat tgatgaaaag
      421 tcgtatacat tcaatcaact cactcaatga atccggattt ttatatagat tcaattcaaa
      481 aagttgcctc gtccctacaa cacgggcttt ttcgtccacc aacgcaatcc tggtgactaa
      541 ttgatccatg gccacactga gaatctgaaa actcccgtct gccagcgaat atgtctgtgg
      601 ccttctacca ccggtcgaac cggccaaacc ggtctcctct attcttcctt cctggattaa
      661 ttcggtaagc agttgagtgg taacgggcag acttttgtca atggcattgc tgatctctga
      721 actagtcaaa cccggagtaa aaaataattg acggaggatt ttaatccgcg caattttgtt
      781 tttcaggttt gtaatttcag ccattttatt taccaaaaga ttaacaaaaa tataaaatag
      841 ttacattact tttgtaaaaa tttataaaac ttattaaata tttttgataa ctttgtgtaa
      901 taaaatgatt gtcagatatt tgcgtggtaa taaaatattt aattattttt atttttttct
      961 tttgtaaaaa gaattggata cgcaaagatg ttttaaaaac agtccattta tcaacttttt
     1021 ataacgatga tggatttttt tacaaatatt tgtttgttag ataaaattcg gaattggcct
     1081 gaattttccc agataaaatg ggcaaataga accggaatca tgaaagcaat ccgataaatg
     1141 accgaaaata tttaaggaaa aaaccagaaa atgatattaa atttataaaa aggaaaaaga
     1201 ccattcagcc ctgcctcacc ttttcttgtc atacaatttt tccggtattg tttttttttc
     1261 tgatctattt gatccccggc aacactcagg aaattatttc catcatcccg aaacctgtga
     1321 aactggaacg caaggcggga agttttatcc ttgatgaatc gacaggatac aggcaagctg
     1381 cagattcagg aatcaatcag gctgtcaggt tacttacctc agagtgcaca cgccggtccg
     1441 gcatcaattt aaaaaatact acaaaagctt caaaattcat ttcgctggct attgttcccg
     1501 gtatcattcc ggaacgggag ggatatacct tatctataac gccgtcagcg attactttga
     1561 aagcaggtga cgaatcgggg ttgatccacg gtgtccaaac catccttcag ttattgccgg
     1621 c
//
